LOCUS       DASUDV010000001         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_972014,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000001 DASUDV010000000
VERSION     DASUDV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972014"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..807
                     /locus_tag="VFV30_00005"
     CDS             52..807
                     /locus_tag="VFV30_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX4846496.1"
                     /translation="MNNNRGNNRRRGRGNRQQGGGGQQLNRIDSRARGNAPQLLEKYR
                     KLAHDAHLNGDRVQAEYYLQFADHYFRVIADGRQRQEEQRMRRDDRWEGGAEEGEDNG
                     EFSVEGDFPSFDQPTYSSRPQREDRPRREDRPEREERAPRGEEPEAGDDGEGEGESSA
                     NPYEPPENPFVRDNRPRAGLKPRRDDRRPRREERDAEDAPAGLDPASLPPAISAGRDD
                     SDSAGADAKPRKPRARRKPAADSGEGEALESVS"
     gene            863..2392
                     /gene="lnt"
                     /locus_tag="VFV30_00010"
     CDS             863..2392
                     /gene="lnt"
                     /locus_tag="VFV30_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX4846497.1"
                     /translation="MLLGALAATGFEPMRLWPLALIALAVLIELVARAHSLRQALLIG
                     WLFGVAHFTLGHNWIATAFQHQAEMPAWLGWIAVLGLSLYLAVWPALAAGLAWRFLRT
                     NYATLVTGFAGAWIVTEWCRGWVFTGFPWNPLAAMALGSFERPGLASLAPWMGTYALS
                     GLVVLLSGAWLIAAKRARIDWRGAALVMVPLALFAWFPGASRPTTSEPGPAFTLIQPL
                     TTQAMLHDPAQFEATFQRSMALSQSRQPDATRLVFWPESGLPDFLEAGYPQAWYDATT
                     YGADPALARQRIGRMIGANSILLTGNDRLEIKDGQVVGARAGITAIDASGKVRAMYAK
                     SHLVPYGEYVPLKWLLEPLGLSRLVPGDIEFWPGPGPRTLDLGQWGRPGMLLCYEVIF
                     SGEVVEPGNRPQVLFNPSNDGWYGAWGPPQHLAQARLRAIEEGLPLLRSTTTGISAVV
                     DAHGKVLQSLPLGGAGRLDGVLPAALSPTLFSQLGNKLALGWALLLLALSMVASRRRK
                     G"
BASE COUNT          379 a          805 c          811 g          412 t
ORIGIN      
        1 gtcgcggatt agaaccccgg gcctggctgc cggattaggg aattgacacc cttgaataac
       61 aatcgtggga ataaccgccg gcgcggccgg ggcaaccgtc agcagggcgg tggcggccag
      121 cagctcaatc ggatcgacag ccgggcgcgt ggcaatgctc cccagctgct cgaaaagtat
      181 cgcaagctgg cccacgatgc gcacctcaac ggcgatcggg tccaggccga atactatctg
      241 cagttcgccg accactattt ccgggtgatc gccgacggcc gccagcgcca ggaagaacag
      301 cgcatgcgcc gcgacgatcg ctgggaaggc ggcgccgaag agggtgagga caacggggaa
      361 ttctcggtcg aaggcgattt ccccagcttc gatcaaccca cctattcttc gcgcccgcag
      421 cgcgaagatc gcccgcgccg cgaagaccgc cccgagcgcg aggaacgcgc tccgcgtggc
      481 gaggaacccg aagccgggga cgatggtgaa ggcgaaggcg aaagctcggc caatccctac
      541 gagccgccgg aaaacccttt cgtgcgcgac aatcgcccgc gcgccgggct caagccgcgc
      601 cgcgacgatc gccgcccgcg ccgcgaggaa cgcgatgccg aagatgcgcc cgccgggctc
      661 gatccggcgt cgttgccgcc cgcgatttcg gccggccgcg atgatagcga cagcgccggg
      721 gccgacgcaa agccgcgcaa gccgcgcgcc cgccgcaagc ccgcagcgga ttccggcgaa
      781 ggcgaagcgc tcgaatcggt gagctgacgc gcgcttgcct cgggcgctta gcctggccct
      841 cgatcacccc cgcaagctgg cgttgctgct aggcgcgctg gccgcaacgg gatttgagcc
      901 catgcggctc tggccgctgg cactgatcgc gctggcagtg ctgatcgaac ttgtcgcgcg
      961 ggcccacagc ctgcgccagg cgctgctgat cggctggctg ttcggcgtcg cgcatttcac
     1021 gctcggccac aactggattg ccaccgcctt tcagcatcag gccgaaatgc cggcctggct
     1081 gggctggatc gcggtacttg gcctgtcgct ctatctggcc gtctggccgg cgctcgcggc
     1141 aggcctggcc tggcgtttct tgcggaccaa ctatgccacg ctggtaaccg gttttgccgg
     1201 tgcctggatc gttaccgaat ggtgccgggg ctgggtcttc accggctttc cctggaaccc
     1261 actggccgca atggcgctgg gatcgttcga acggccgggc cttgcctcgc tcgcgccgtg
     1321 gatggggacc tatgcccttt caggacttgt cgtgctgctt tccggagcct ggctgatcgc
     1381 ggcgaagcgc gcccggatcg actggcgggg cgccgcgctg gtgatggtgc cgctggccct
     1441 gttcgcctgg ttcccgggtg ccagccgccc gacaaccagt gaaccgggcc cggccttcac
     1501 gctgatccag ccactcacca cccaggccat gttgcacgat ccggcgcagt tcgaagccac
     1561 cttccagcgc tcgatggcac tcagccagtc ccgccagccg gatgcgacgc ggctggtttt
     1621 ctggcccgaa tccggcctgc ccgactttct cgaggcaggc tatccgcagg cttggtacga
     1681 tgccacgacc tatggcgcgg accctgccct cgcccgccag cggatcggcc ggatgatcgg
     1741 cgcgaacagc atcctgctga ccggcaacga ccgacttgag atcaaggacg gacaggtggt
     1801 gggtgcgcgg gccgggatca cggcgatcga tgcgtcgggc aaggtgcggg cgatgtatgc
     1861 caaatctcac ctggtgccct acggcgaata tgttccgctc aagtggctgc tcgagccgct
     1921 gggactgtcc cgtctggtgc ccggtgacat cgaattctgg cccggccctg gtccgcgcac
     1981 gcttgatctt gggcagtggg gccgaccggg catgttgctg tgctacgagg tgatcttctc
     2041 gggcgaggtg gtcgaaccgg gcaatcggcc gcaggtgctg ttcaatccgt cgaacgatgg
     2101 ctggtacggt gcctggggcc cgccccagca cctggcccag gcccggctgc gcgcgatcga
     2161 ggaaggcctt ccgctgctgc gttcgaccac gaccgggatc agcgcagtgg tcgatgccca
     2221 tggcaaggtg ctgcaatcgt tgcctttggg aggggccgga cggcttgacg gcgtgcttcc
     2281 cgcagcgctt tccccaacct tgttttcgca gctgggcaat aaacttgcgc ttggctgggc
     2341 actgctcctt cttgcgttga gcatggttgc atcgcggcgg cgcaagggtt agagcgcagc
     2401 atcacat
//
LOCUS       DASUDV010000002         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1369706,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000002 DASUDV010000000
VERSION     DASUDV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369706"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFV30_00015"
     CDS             complement(<1..221)
                     /locus_tag="VFV30_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX4846498.1"
                     /translation="MIRRVLAIFALVFALPLAAQDAVPPAPYANVQLADPAQEAKAHA
                     LMLTLRCLKCQSQSIADSDAPMAGDMRSLV"
     gene            complement(218..766)
                     /locus_tag="VFV30_00020"
     CDS             complement(218..766)
                     /locus_tag="VFV30_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX4846499.1"
                     /translation="MSETKPTARWTLWLPLALFLGFIALVMIGLYRPADREVASTMIG
                     KPLPEFALRAAVVDRPGLARADLADGKPRLLNIFASWCVPCAVEAPQLATLKDEGIEI
                     AGVAIRDRPEDVANFLARHGNPFTRIGADDRSIVQLGIGSSGVPETFVIDGKGTIRYQ
                     HIGEIRPEHVALLREKLKEAGQ"
     gene            complement(763..>2467)
                     /locus_tag="VFV30_00025"
     CDS             complement(763..>2467)
                     /locus_tag="VFV30_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX4846500.1"
                     /translation="FIYRFSGAWGNHEGSMLLWVTVLSAAGALLALVERRLPERSLRA
                     TLGAQALLALGFFGFLLLASNPFTRLDPAPLNGQGLNPLLQDPGLAFHPPPLYIGYVG
                     LSVAFSFAIGALVTREVGPAFARAMRPWVLGAWIFLTLGITAGSYWAYYELGWGGWWF
                     WDPVENASLMPWLAATALLHSVSVLAARNALRAWTVMLGVVAFSMSMVGTFLVRSGIL
                     TSVHAFAVDPERGSFILGLLALNIGGALVLFGLRASSVTEGERFTATSREGALVFNNV
                     MLSAILGIVLVGTLYPLLAEALGAKVSVGPPYFNPVSAIFAVPMLLVLIVGPLLRWRR
                     DSLSRIRLQIAIPAVLLVGTLVWAASQKVGLLPALGLALSAALAVASLLPLRGRKLRQ
                     VKLPLWGMVTAHFGIAVALLGMASDAAFTTERLVAVQAGQVVDVGPWQVTLQRIEPVA
                     GPNWTALRGDLAVSYKGGAVSELHPAARTFWAPPNETNEAALKTRWNGQLYAVLGQAT
                     GDGRWQLRLWWKPFVPMIWYGGILIALGGLMALFGRLASDLKRLVARDKIAYRRMRQG
                     R"
BASE COUNT          473 a          864 c          803 g          327 t
ORIGIN      
        1 acgaggctgc gcatatcgcc ggccatcggc gcgtcggaat cggcgatcga ctggctctgg
       61 cacttgaggc agcgcagggt cagcatcagc gcatgggcct tggcctcctg agccggatcg
      121 gccaactgga cattggcata gggcgctggc ggaacggcat cttgcgccgc tagcggcagc
      181 gcgaagacca gcgcgaagat cgccagcacg cgacggatca ttgccccgcc tccttcagct
      241 tttcgcgcag cagggcgaca tgttcgggcc ggatctcgcc aatgtgctgg taacggatcg
      301 ttcccttgcc atcgatcacg aaagtttccg gcacgcccga tgagccaatc ccaagctgga
      361 caatcgagcg atcgtccgcg ccaatccggg tgaacggatt gccgtgccgc gcaaggaaat
      421 tggcgacgtc ttccggccgg tcgcggatcg ccacgccggc aatctcgatc ccttcgtcct
      481 tgagcgtggc gagctgcggc gcttctaccg cgcagggcac gcaccatgag gcaaagatgt
      541 tgagcaggcg cggcttgcca tcggcgagat ccgcccgggc caaccccggc cggtccacca
      601 cggcggcgcg cagggcgaat tcgggcagcg gcttgccgat catggtgctc gccacttcgc
      661 gatcggccgg acgatagagc ccgatcatca ccagcgcgat gaagcccagg aacagcgcca
      721 gcggcagcca cagcgtccag cgcgcggtcg gcttggtctc gctcatcggc cctgcctcat
      781 ccggcgatag gcaatcttgt cgcgcgccac cagccgctta agatcgctgg ccaggcgccc
      841 gaacagcgcc atcaggccgc ccagtgcgat caggatgccg ccgtaccaga tcatcggcac
      901 aaacggcttc caccacagcc gcagctgcca gcgcccgtca ccggttgcct ggcccagcac
      961 ggcatagagc tggccgttcc agcgggtctt gagcgcagcc tcgttggttt cgttcggcgg
     1021 cgcccagaag gtccgcgccg ccggatgcag ttcgcttacc gcgccgccct tgtagctaac
     1081 cgccagatcg ccgcgcagcg cggtccagtt ggggccagcg accggttcga tccgctggag
     1141 ggttacctgc caggggccaa catcgaccac ctgccccgcc tgcactgcca ccagccgctc
     1201 ggtggtaaag gccgcatcgc tcgccatgcc aagcagcgcc acggcaatcc cgaaatgggc
     1261 tgtaaccatg ccccacagcg gcagcttgac ttgccgcagc ttgcgtccgc gcagtggcag
     1321 caggctggca acagcaagtg ccgctgacag cgccagtccc agcgcgggca gcagccccac
     1381 cttctggctc gccgcccaga ccagcgtacc gaccagcagg acggctggaa tggcaatctg
     1441 aagccggatc cggctcaagc tgtcccgccg ccagcgcagc agcggcccga cgatcaggac
     1501 cagcagcatc ggcacggcaa aaatcgcgct gaccgggttg aaatagggcg ggccgaccga
     1561 aaccttggca cccagcgctt cggccagcag cggatagagc gtgccgacca gcacgatgcc
     1621 caggatcgcg ctgagcatga cattgttgaa caccagcgcg ccttcgcggc tggtcgcggt
     1681 gaagcgttcg ccctcggtca cgctcgaggc gcgcaggccg aacagcacca gcgcgccgcc
     1741 gatgttgagc gccagcagac ccaggatgaa gctgccgcgc tccggatcga ccgcgaaggc
     1801 gtggaccgag gtgaggatgc ccgagcggac caggaaggtc ccgaccatcg acatcgaaaa
     1861 ggccaccacg cccagcatca ccgtccaggc gcgcagcgca ttgcgcgcgg cgaggaccga
     1921 aaccgaatgg agcaaggccg ttgccgccag ccatggcatc aggctggcgt tctcgaccgg
     1981 atcccagaac caccagccgc cccagcccag ttcgtaatag gcccagtagg aaccggcggt
     2041 gatccccagc gtcaggaaaa tccacgcgcc cagcacccag ggccgcatcg cgcgggcgaa
     2101 agccgggccg acctcccgcg tgaccagcgc gccgatcgcg aagctgaaag ccaccgacag
     2161 cccgacatag ccgatgtaca ggggcggcgg gtgaaaggcg aggcccgggt cctgcagcag
     2221 cgggttcagc ccctgcccgt tcagcggcgc ggggtcgagc cgggtgaatg gattggaagc
     2281 gagcagcagg aagccgaaga agccgagcgc cagcagcgcc tgggcgccga gcgtggcgcg
     2341 cagagagcgc tccggcagcc gccgctcgac cagggcgagc agtgcgcccg cggccgagag
     2401 aacggtgacc catagcagca tcgagccttc atgattcccc cacgcgcccg aaaagcggta
     2461 gatgaag
//
LOCUS       DASUDV010000003         6729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2164963,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000003 DASUDV010000000
VERSION     DASUDV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164963"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFV30_00030"
     CDS             complement(<1..562)
                     /locus_tag="VFV30_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4846501.1"
                     /translation="MKIAVPIHSFEPGGVERVALNLCAAWQAEGTDVTVVLGRDEGAM
                     KATAPALAYHLLPEPVPTAAFETLWMIRVLRRYLARTPADVIWASGNTYAIVAVAMKL
                     LLGRRCPPVAIKLSNDLYRKDMIAPYRAAYHWWLRLQGRMLDHFVGMAQPMRAEIAEL
                     MRVPDTKITIIEDPSLAESQFAALSAL"
     gene            complement(559..1296)
                     /locus_tag="VFV30_00035"
     CDS             complement(559..1296)
                     /locus_tag="VFV30_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4846502.1"
                     /translation="MSKLLLASIHDVGPRFEREVDQLAEQLARILGGPKFAMLVVPDH
                     WGEAPLAGNTAFQRRLRDWADQGVEMFVHGWFHKDLAEHSGAAAFKAKHMTAGEGEFL
                     GLSREEAGRRMAEGRKLVEDAIGRPAAGFIAPAWLYGEGALAALADNGFALAEDHMKV
                     WQPGSEKVLAKGPVITWASRTVPRQLSSRFFAGLARTFLHPQRIMRIAVHPGDTTVPA
                     LIDSIEATYSAFAARRPAGRYADLLPA"
     gene            complement(1453..2442)
                     /locus_tag="VFV30_00040"
     CDS             complement(1453..2442)
                     /locus_tag="VFV30_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX4846503.1"
                     /translation="MTQDAVRMPPEWHPQAWLWVGFPHDAEEWPGFLGEAQVQIAAFA
                     SAVADSGQEVRLIVRDEATRDRAARLCSSAVKLEVRRYGDIWLRDTGPLVVERGGRAV
                     GQRFGFNGWGGKYLMDGDQEIGAELAESAGLPVATADWVLEGGALDCDGTGLVATTEQ
                     CLLNPNRNPQLSRADLEARLHRDLGFDRVLWLGDGLINDHTDGHVDNLARFVAPNVLA
                     LPRATGADDPNAAIYADAKARAEAFGVTVREVPSPGRVETDGRIEPASYMNFAVTSNL
                     VVVPTYGTAHDADGVAAIAELFPDRDVVGILATAVLAGGGSFHCASQQMPRLP"
     gene            complement(2447..4141)
                     /locus_tag="VFV30_00045"
     CDS             complement(2447..4141)
                     /locus_tag="VFV30_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX4846504.1"
                     /translation="MKFTRTTLVSVVALGSAVALAAGVSARSPKGGALPVPKVAAPVI
                     QSDTLNTITRELSSDAYEGRGPATPGEEKTVAYIIQRYKDAGLQPGNNGSWTQDVPTV
                     EIAAQNVTPMTVNAGGKAMAFEFGKDWVAGSYRAVEKTAIADAPMVFVGYGINAPELG
                     WNDYAGVDMKGKIAVILVNDPDYEAKDEKGLFKGKRMTYYGRWTYKFEEAARQGAAGA
                     LIVHDTYPAAYGWNVVNSSWTGPQLYIQTPDKGMSNTQANGWIQKDVAAQILKASGQD
                     LDALSKAAKQKGFKPVPLEAKGSLGFDNTIRTAMSRNVVGILPGKKRPDEYVLYTAHW
                     DHLGRCTPDETGDDICNGAIDNATGVAALVAIAEANRKAGPADRSQVFLAVTLEESGL
                     LGSKYYAENPIYPLAKTVGGVNMDALSPGSVARDVQVTGGDKSELTRYLRATMAAMKL
                     YETPEDHSERGYYYRSDHFSFAKQGVPMFYLGRGSDWVHGGKAAAEAMAEEYTAKHYH
                     RPSDEWSAVWDWSGMVQDVTLYYRLGRSLAMSTEWPNWYKTDEFRLIRDKSRAGGK"
     gene            4266..>6729
                     /locus_tag="VFV30_00050"
     CDS             4266..>6729
                     /locus_tag="VFV30_00050"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX4846505.1"
                     /translation="MSADWTHAIARARAHAPFLAQGLDRLPALEALLAAGQGEAALAW
                     ARAAGEGAPDTGSALRREKLALATALGVGDLAGAFPLGRVVAELSGFADRALDTAIAA
                     AIRARVPDAEPAGFIALALGKHGAEELNYSSDIDPILLYDPATLPRRERDEPGEAAQR
                     VARTLMQLMSHVDHEGYVFRVDLRLRPASEVSPLAIPIGAALTHYESSALAWERAAFI
                     RARACAGDIPAGEAFLAAIRPFVWRRSLDFGAIAEIGRLTQRIRANHTGPVRPGPGFN
                     LKQGRGGIREVEFFAQTHQLIHGGRNPALRLRGTRASLDALAEAGLIAAEDARVLGDS
                     YDRLRTLEHRLQMVADQQTHSLPSDPAALDNVARLEGLPDGAALVAELEAITEAVGTR
                     YDGLIAQYAEEGVAAPRATGGAALTEKLAGMGFADPEGLSARIEGWRSGKLRALRSDA
                     AREAFDAMLAPLLAAFAKAPEPERALLRWEQLLTNLPSAVNLFRLLEARPALAELLAR
                     ILGLAPPLADALARRADLLDPLIDASAFDLPGSTEDLVARLSAGEADDDYERLLDRVR
                     RRVGELRFKLGVQLIEGSRDPAEIGQALCRVAEAALTVLTRAATAEFERVHGKVPGGE
                     LVIMGYGRLGGGALTHASDLDLVFLFTGDHGAESDGARPLGATLYFNRLAQRVIAALS
                     VPTAEGALYEVDTRLRPSGAQGPIAVSLDAFARYQRETAWTWEHMALARARVLHGPEA
                     GRAELDAIIRAVLELDRDPAKLRADVLEMRAAMAAHKPAKGPLDVKLARGGLVDIEFL
                     VHYLQLRDHRALVPSIGAAVAAL"
BASE COUNT          973 a         2351 c         2349 g         1056 t
ORIGIN      
        1 gcagggcaga cagggcggcg aactggcttt cggccagcga ggggtcctcg ataatcgtga
       61 tcttcgtgtc cggcacccgc atcagctcgg caatctcggc ccgcatcggc tgggccatgc
      121 cgacgaagtg atcgagcatc cgcccctgca ggcgcagcca ccagtgatag gcggcgcggt
      181 agggggcgat catgtccttg cggtaaagat cgttcgacag cttgatcgcc accggcgggc
      241 agcgccgccc cagcagcagc ttcatcgcca cggcaacgat cgcataggtg ttgcccgagg
      301 cccagatcac gtcggccggg gtgcgggcca gatagcggcg caggacccgg atcatccaca
      361 gcgtctcgaa cgccgcggtc gggaccggtt cgggcagcag gtgataggcc agcgccggcg
      421 cggtggcctt catcgccccc tcgtcgcgcc ccagcaccac ggtgacgtcc gtcccctcgg
      481 cctgccaggc ggcgcagaga ttgagcgcga cccgctcgac cccgccgggt tcgaacgagt
      541 ggatcgggac ggcgatcttc atgccggcag cagatccgcg tagcgcccgg ccggacggcg
      601 cgcggcgaag gcggaatagg tcgcctcgat gctgtcgatc agggccggaa cggtggtatc
      661 gccgggatgc accgcgatcc gcatgatccg ctgcgggtgc aggaacgtcc gcgcgaggcc
      721 ggcaaaaaag cgcgaggaca gctggcgcgg aaccgtgcgg ctggcccagg tgatcaccgg
      781 gcctttggcg agcaccttct cgctgcccgg ctgccacacc ttcatgtgat cctcggccag
      841 cgcaaagccg ttgtcggcca gcgccgccag ggcgccttct ccatagagcc aggccggggc
      901 gatgaagccc gcggcggggc ggccgatcgc gtcctccacc agcttgcgcc cctcggccat
      961 gcggcggccg gcctcttcgc gcgacaggcc caggaactcg ccttcgccgg cggtcatgtg
     1021 cttggccttg aaggccgccg cgccggaatg ctcggccaga tccttgtgga accagccgtg
     1081 gacgaacatt tccacgccct gatcggccca gtcccgcagg cggcgctgga aggcggtgtt
     1141 gccggccagc ggcgcttcgc cccagtgatc ggggaccacc agcatggcga atttcggacc
     1201 gcccaggatc cgcgccagct gctcggccag ctggtcaacc tcgcgctcaa agcgcgggcc
     1261 gacatcgtgg atcgaggcga gcaggagctt ggacatggac cggctcctat cgcatccgca
     1321 gtccaaaggg aatcgcctta ccccaccccg ttagtcctga gcttgtcgaa ggactgtcct
     1381 tcttggcgag gtcgcagaga aagaagacgg cccttcgaca agctcagggc gaacggtttg
     1441 gggtgggtga ggtcagggca accgcggcat ctgctggctg gcgcagtgaa agctgcctcc
     1501 gccggccagg acggccgtgg ccagaatgcc caccacgtcg cggtccggga acagctccgc
     1561 gatggcggca acgccatcgg catcgtgggc cgtaccatag gtcggcacca ccaccaggtt
     1621 cgaagtgacg gcgaaattca tgtagctcgc cggctcgatc cgcccgtccg tttccacccg
     1681 cccgggggag ggcacctcgc gcacggtcac gccaaaggct tcggcccggg ccttggcatc
     1741 ggcatagatc gcggcgttgg gatcgtccgc cccggtggcg cgcggcaagg ccagcacgtt
     1801 cggcgcgaca aaccgcgcaa ggttgtcgac atggccgtcg gtgtgatcgt tgatgaggcc
     1861 atcgcccagc cacagcaccc ggtcaaagcc gagatcgcgg tggaggcgcg cttcgaggtc
     1921 cgcgcggctc agctgcgggt tgcggttggg gttcagcagg cactgctcgg tcgtggcgac
     1981 gaggcccgtc ccgtcgcaat cgagcgcccc gccttccagc acccagtccg ccgtggcgac
     2041 cggcaggccg gcgctttcgg ccagctctgc gccgatctcc tgatcgccgt ccatcaggta
     2101 cttgccgccc cagccgttga agccaaagcg ctgaccgacg gcgcggccgc cgcgctccac
     2161 caccagcggg ccggtgtcgc gcagccagat gtcgccatag cggcgcactt ccagcttcac
     2221 ggcggaagag cacagccggg ccgcgcggtc ccgcgtggcc tcgtcgcgca cgatcagccg
     2281 cacttcctgg ccgctgtcgg cgacagcgct ggcaaaggcc gcgatctgga cctgggcctc
     2341 acccagaaag cccggccact cctccgcgtc gtggggaaag ccgacccaca gccaggcctg
     2401 cggatgccac tccggcggca tccgcaccgc gtcctgcgtc atcgccttac ttcccgcctg
     2461 cgcggctctt gtcgcggatc aagcggaact cgtcggtctt gtaccagttc ggccattcgg
     2521 tgctcatcgc caggctgcga ccgagccggt aatagagcgt gacgtcctgg accatgccgg
     2581 accagtccca cacggcgctc cattcgtcgc tggggcggtg atagtgcttg gcggtgtatt
     2641 cctcggccat cgcctcggcc gcggccttgc cgccgtggac ccagtcggac ccgcggccca
     2701 gatagaacat cggcacgccc tgcttggcga agctgaagtg gtccgaacgg tagtaatacc
     2761 cgcgctcgga atggtcttcc ggggtttcgt aaagcttcat cgccgccatg gtcgcgcgca
     2821 gatagcgggt cagttccgac ttgtccccgc cggtcacctg cacgtcgcgg gccacgctgc
     2881 cgggcgacag cgcgtccatg ttgaccccgc caacggtctt ggccagcgga tagatcgggt
     2941 tttcggcata gtacttggag ccgagcaggc ccgattcctc cagcgtcacg gccaggaaca
     3001 cctggctgcg gtcggccggg ccggccttgc ggttggcctc ggcaatggcg accagcgcgg
     3061 ccaccccggt ggcgttgtcg atcgcgccgt tgcagatgtc atcgccggtc tcgtccgggg
     3121 tgcagcggcc caggtgatcc cagtgcgcgg tgtagagcac gtattcatcc ggccgcttct
     3181 tgccgggcag gatgcccacc acgttgcggc tcatcgcggt gcggatggtg ttgtcgaaac
     3241 cgaggctgcc cttggcctcc agcggcaccg gcttgaagcc cttctgcttg gccgccttgc
     3301 tcagcgcgtc gagatcctgg cccgaggcct tgaggatctg cgctgccacg tccttctgga
     3361 tccagccgtt ggcctgggtg ttgctcatcc ctttgtccgg ggtctggata tagagctgcg
     3421 ggccggtcca gctggaattg accacgttcc agccataggc ggccggatag gtatcgtgga
     3481 cgatcagcgc gcccgccgcg ccctgccggg cggcttcctc gaacttgtag gtccagcggc
     3541 cgtaataggt catccgcttg cccttgaaga ggcccttctc gtccttggcc tcgtaatccg
     3601 gatcgttgac caggatgacc gcgatcttgc ccttcatgtc gacgccggca tagtcgttcc
     3661 agcccagttc cggggcgttg atgccatagc cgacgaagac catcggcgcg tcggcgatcg
     3721 cggttttctc gaccgcgcgg taggaacccg cgacccagtc cttgccgaat tcgaaggcca
     3781 tcgccttgcc gccggcgttg accgtcatcg gggtcacgtt ctgcgcggcg atctccacgg
     3841 tcgggacgtc ctgggtccag ctgccgttgt tgcccggctg cagcccggca tccttgtagc
     3901 gctggatgat gtaggcgacg gtcttttcct cgcccggggt ggccggaccg cggccttcat
     3961 aggcatcgga cgacagctcg cgggtgatgg tgttgagcgt gtcggactgg atcaccggcg
     4021 cggcgacctt gggcaccggc agcgcgccgc ccttgggcga gcgggccgaa accccggcgg
     4081 ccagcgccac ggccgagccg agcgcgacga cggaaacgag cgtggtgcgg gtaaacttca
     4141 tgggggcgat accgtctttc gctgaaggag gagtgcggct gtctggcaaa gcgccttgcc
     4201 ccatgcaagg gccgatccga cgaacgactt gatcaaccca gcgcaaagcg gcatcgccgg
     4261 tgccgatgag tgccgactgg acccacgcaa tcgcccgcgc ccgcgcccat gcgccgttcc
     4321 tggcccaggg gctggaccgg ctccccgcgc tggaagcctt gctggccgcg ggacagggcg
     4381 aagcggcgct ggcctgggcc cgcgcggcgg gtgagggcgc gcccgacacc ggatcggcgc
     4441 tgcggcgcga aaagctcgcc ctggcgaccg cgctgggggt gggggatctg gccggcgcct
     4501 ttccgctggg gcgggtcgtg gcggaactgt cggggtttgc cgaccgggcg ctcgacaccg
     4561 cgattgccgc cgcgatccgc gcgcgggtgc ccgatgccga gccggccggg ttcatcgccc
     4621 tggcgctggg caagcacggc gctgaggagc tgaactactc gtccgacatc gacccgatcc
     4681 tgctctacga tccggcaacc ctgccccggc gcgagcggga cgagccgggc gaggcggcgc
     4741 agcgggttgc ccgcacgctg atgcagttga tgagccacgt cgaccacgaa ggctatgtct
     4801 tccgggtcga cctgcggctg cgcccggcca gcgaggtcag cccgctggcg attcccatcg
     4861 gcgcggcgct aacccattac gaaagctccg cgctcgcctg ggaacgcgcc gccttcatcc
     4921 gcgcccgggc ctgcgccggg gatattccgg cgggcgaagc ctttctggcc gcgatccggc
     4981 ccttcgtctg gcggcgcagc ctggatttcg gggcgattgc cgaaatcggg cggctgaccc
     5041 agcggatccg cgccaaccac accggccctg tccgccccgg ccccggcttc aacctgaagc
     5101 aggggcgcgg cgggatccgc gaggtggagt tcttcgccca gacccaccag ctgatccacg
     5161 gcgggcgcaa cccggcgctg cggctgcgcg gcacccgggc cagcctggat gccctggccg
     5221 aggccgggct gatcgcggcc gaggacgcgc gggtgctggg cgacagctat gaccggctgc
     5281 gcacgctgga gcaccggctg cagatggtgg ccgaccagca gacccattcg ctacccagcg
     5341 atccggccgc gctcgacaat gtcgcccggc tggagggcct gcccgatggc gcggcgctgg
     5401 tcgcggaact ggaggcgatc accgaagcgg tcggcacccg ctatgacggg ctgatcgccc
     5461 aatatgccga agagggcgtg gccgcccccc gcgcgaccgg cggcgcggcg ctgaccgaaa
     5521 agctggccgg catgggcttc gccgatcccg aagggctttc ggcgcggatc gagggctggc
     5581 gctcgggcaa gctgcgcgcc ctgcgcagcg atgccgcgcg tgaggctttc gatgccatgc
     5641 tggcgccgct gctcgccgct ttcgccaagg cgccggagcc ggagcgggcg ctgctgcgct
     5701 gggagcagtt gctcaccaac ctgcccagcg cggtcaacct gttccgcctg ctggaagccc
     5761 gcccggcgct ggccgagctg ctggcccgga tcctcggcct cgccccgccg ctggccgatg
     5821 ccctggcgcg gcgggccgac ctgctcgacc cgctgatcga tgcttcggcc ttcgatctgc
     5881 ccggatcgac cgaggacctg gtcgcgcgcc tgtcggcggg ggaggccgac gacgattacg
     5941 agcgcctgct cgaccgggtc cgccgccggg tcggcgaact gcgcttcaag ctgggggtcc
     6001 agctgatcga gggcagccgc gatccggccg agatcggcca ggcgctgtgc cgggtcgccg
     6061 aagccgcgct gaccgtcctg acccgcgccg ccacggccga gttcgagcgg gtgcatggca
     6121 aggtgccggg cggcgagctg gtgatcatgg gctacggccg gctcggcggc ggggcgctga
     6181 cccatgcctc ggacctcgac ctcgtgttcc tgttcaccgg cgatcacggc gccgaaagcg
     6241 acggggcgcg gccgctgggc gcaacgctct atttcaaccg gctggcccag cgggtgatcg
     6301 cggcgctctc ggtcccgacg gcggagggcg cgctttatga agtcgacacc cggcttcgtc
     6361 cgtccggcgc gcaggggccg attgcggtca gcctcgatgc ctttgcccgc taccagcgcg
     6421 aaaccgcctg gacctgggaa cacatggcgc tggcccgggc gcgggtgctg cacgggccgg
     6481 aggcgggccg ggccgaactg gacgcgatca tccgcgcagt gctggaactg gaccgcgatc
     6541 ccgccaagct gcgcgccgac gtgctggaga tgcgcgccgc catggctgcg cacaagcccg
     6601 ccaagggccc gctcgacgtc aagctggcgc ggggcgggtt ggtcgatatc gaattcctgg
     6661 ttcactacct gcagctgcgc gatcaccgcg cgctggttcc gtcgatcggg gccgccgttg
     6721 ccgccctgg
//
LOCUS       DASUDV010000004         7558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_817443,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000004 DASUDV010000000
VERSION     DASUDV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817443"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1511)
                     /locus_tag="VFV30_00055"
     CDS             complement(12..1511)
                     /locus_tag="VFV30_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX4846506.1"
                     /translation="MQDPHSISQIVIVGGGTAGWMTAAALANAIRTGCAITLIESDEI
                     GTVGVGEATIPPIRTFNEALGITENEFVAATQGSFKLGIQFVDWGKRDHRYFHPFGTY
                     GRNFDFLPLHQYWLAAQARGEAPNLDDLCMAWAAAKRGKFAQPSPEQRNVLSTFDYAY
                     HFDAGLYARFLRNYAEARGVVRIEGKVGDVALDGESGHVRHVTLQDGRQIEGQLFVDC
                     SGFRGLLIEGALQTGYQDWRHWLPCDSAMAVPCERAGEFTPYTRSTARQAGWQWRIPL
                     QHRTGNGHVYCSQYISDDEAAAVLMANLDGKALADPRKLSFVTGRRNLFWNKNVVAVG
                     LSGGFMEPLESTSIHLIQAAIAKLIALFPDRHFDPLVIDEYNRLAIAEFERIRDFIIL
                     HYKLTSRTDSELWRYCAAMEVPETVTWKIEHFRRNGRLVQRDADLFGPPSWLAVHVGQ
                     GNMPQGTDPLLPLRGVDHADYLRKLEAALARAAEGMPGHADYIARNCAA"
     gene            complement(1521..2900)
                     /locus_tag="VFV30_00060"
     CDS             complement(1521..2900)
                     /locus_tag="VFV30_00060"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX4846507.1"
                     /translation="MDSADPQTLRSRALALCAEQRLDEAERDLAAAVARFPGDPGLAF
                     VHAQVRYERGYPAADLFARAEALAPDHPEIVRNYALALLSEGRRDAARARLEARLARH
                     PDWLDGHKALATLNWTGGDQTGFSDHLAAACRAQPDNAALWLAWFRNLAQARQWQAAS
                     AVLDEAERHLGPRPDLAVSRLFVAVESAAPDAARHIAATAAIRGDVASLCRIRHALRQ
                     GDARTAEGEALPLTAGPSAALYWPYLSLCWRLLGDPRAEWLDRPADLIRSFDAGIDPA
                     ELAALADLLRGLHTASQPYIEQSVRGGTQTDRSVLLRHEPELQRLKARLLAVIRDYSE
                     GLPPNDPAHPLLSAPRDGRLLIAGSWSVRLHRLGHNVPHTHAMGWLSTAFYVALPDPV
                     AMGPAPAGHIAFGAPPPELGLDLAPYRTLAPQAGHLAVFPSTLWHSTVPFDDGERLVV
                     AFDIRRPTV"
     gene            complement(3011..5491)
                     /locus_tag="VFV30_00065"
     CDS             complement(3011..5491)
                     /locus_tag="VFV30_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4846508.1"
                     /translation="MALVAAPAFAQDAPADEAEATGLDEIVVTASGRDKTKLDSSVSS
                     TSVDASLIEGMNPSSEAEVFRMIPGIQVPGTSGPGGNSNIAVRGLPVATGGSPFVQIQ
                     EDGLPTVLFGDIQFGNNDYWTRFDASVERVESVRGGGATTYASQAPGAVINYISNTGA
                     KEGGFVRVSKGVNYDETKIDFRYGTSLAEDLRFHIGGSFKTGRGPLHADYNVSDSIQV
                     KGNITKDLPDGRGYFRLLFKVADTKEPNYTGAPALATLSGGKFSNVQPFPGFDGRNSS
                     NYSIYNQSFLILGRSGQLERVGMDGIRTKATSLGSQFHYEFGENITVDNNMRWTKMSG
                     SFTSPFQNTSRASGIVGSNLTINGTPYATVASIRYANGPRAGQVFTGTYVDNNTNVHT
                     NIRDMGSLANDLTLAGKFDAGSMKLTVRGGFFYMNQKIAMDWHTNKSLREVSGNNPAQ
                     LDLYDGAGNKLTQDGISGYNNNWGNCCARDYDLSYTNTAPYAALELDSDDFNLDGSVR
                     FENVKASGTGTAGGSEFFVNSNGVLIPAITSNGTRETLNYSRSYTSWTAGALWKVNGN
                     TSVFVRASRGGRFNGDRQTFGGKIASDGSLCTGAMAAAGTMGCVADGVTPSVDFVKQY
                     ELGLKNRGEMAGGRYTLELTLLKGSFKQSTFELSATRCPGGSGGCVIDARYKSYGLEF
                     YGTYRSGGFSLVGNATWTKAERSGVDGSGNPTPYARAQYMPDLIYTVQASYDFSDIVT
                     LGVSATGQSGIVDDGGRGYPGGAVIGLDFSIRPIPNLELGINGYNIFNRYDLRGSGGP
                     ADLSVSPGVIGTGPALGRTWTASIKYSF"
     gene            5817..6839
                     /locus_tag="VFV30_00070"
     CDS             5817..6839
                     /locus_tag="VFV30_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4846509.1"
                     /translation="MSEDTPPPTRIRNIAELARLAGVSAGTVSRALADMPLVNKETRR
                     RIQALAREHGFRPNQMARRLRVQETKVIGVVVPLGHEQRQHLSDPFFMAILGHLADAL
                     TENGYDIMLSRVIPDAEDWLERIVSSGMLDGVLLIGQSNQFDAIERVAQWYRPMVVWG
                     SNRPGQAHCTVGVDNRKGGRLAGECLIASGRKRLAFMGEKIAPELSERYLGLCDAAAA
                     AGLPPPLQLDTHMASDVMADEIAGHIARIAAEVDGIAAASDLIAMHTVRALADRGIAV
                     PGQIAVTGFDDLPLAEQTIPRLTTISQNVPAGAKAMVKALFDRIAGIDAASLEMAPEL
                     VRRDTA"
     gene            complement(6836..>7558)
                     /locus_tag="VFV30_00075"
     CDS             complement(6836..>7558)
                     /locus_tag="VFV30_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846510.1"
                     /translation="ELPVEGETGTRWLFKVDVLRGAPGSGALYRTGHFDGTRFVSDAN
                     EDWRVADWGNDFYAAIAWHEPRDAAGRPAWIGWIGNHAYQGQLPRQGWRGAMSTPRRL
                     SLVRTGEELVLRQEVEPALVPQVPLADASAAQALFAARLIQPGGADFALAIRDGEGRA
                     VQVTRQGARLVLERTDPQSPFLDGSWQAEADPADPVEIWIDGGSLEVLAGRGCAALTV
                     QHRLSGAEYRLERAEPAAASAA"
BASE COUNT         1190 a         2469 c         2678 g         1221 t
ORIGIN      
        1 aagctcctcg cctaggccgc gcagttgcgg gcgatgtagt ccgcatggcc gggcattccc
       61 tccgcggcgc gggccagcgc cgcttcaagt tttctgagat agtccgcgtg atcgaccccg
      121 cgaagtggca gcagcggatc ggtgccttgg ggcatgttgc cctggccgac gtggaccgcc
      181 agccagctgg gcggcccgaa caggtcggcg tcgcgctgca ccagccggcc attgcggcgg
      241 aaatgctcga tcttccaggt gaccgtttcc ggcacctcca tcgccgcgca atagcgccac
      301 agctcgctgt cggtgcggct ggtcagcttg tagtgcagga tgatgaaatc gcggatccgc
      361 tcaaactcgg cgatggccag gcggttgtat tcgtcgatca ccagcgggtc gaagtgccgg
      421 tcggggaaca gcgcgatcag cttggcgatg gcggcctgga tcaggtggat cgaggtcgat
      481 tccagcggct ccatgaaccc gcccgacagg ccgacggcga ccacgttctt gttccagaac
      541 aggttgcgcc ggccggtcac gaacgaaagc ttgcgcggat cggccagggc cttgccatcg
      601 aggttggcca tcagcaccgc cgccgcctcg tcgtcggaaa tgtactggct gcaatagacg
      661 tggccgttgc cggtgcggtg ctgcagcggg atgcgccact gccacccggc ctgccgcgcg
      721 gtggagcggg tgtagggggt gaattcgccg gcccgctcgc agggcacggc catggcgctg
      781 tcgcagggca gccagtggcg ccagtcctga tagccggtct gcagtgcccc ttcgatcagc
      841 agcccgcgaa agccggagca gtcgacgaac agctggcctt cgatctgccg cccgtcctgc
      901 agggtgacat ggcggacatg gccgctttcc ccgtccagcg cgacatcgcc aaccttgccc
      961 tcgatccgca ccacgccgcg cgcttcggcg taattgcgca ggaaccgggc atagagcccg
     1021 gcatcgaagt ggtaggcata gtcgaaggtg gagaggacat tgcgctgttc gggcgagggc
     1081 tgggcaaact tgccgcgctt ggccgcggcc caggccatgc acaggtcatc caggttgggc
     1141 gcttcgccgc gggcctgggc ggccagccag tactggtgca gcggcaggaa atcgaagttg
     1201 cggccatagg tgccgaaggg gtggaaatag cggtgatccc gcttgcccca gtcgacgaac
     1261 tggatcccca gcttgaacga gccctgggtc gcggcgacga attcgttctc ggtgatcccc
     1321 agcgcctcgt tgaaggtgcg gatcggcggg atcgtggctt cgcccacgcc cacggtgccg
     1381 atttcgtcgc tctcgataag ggtgatcgcg cagccggtgc ggatcgcgtt ggccagggcg
     1441 gcggcggtca tccagccggc ggttccgccg ccgacaatca cgatctggct gatcgaatgg
     1501 gggtcttgca tcacggtgtc ctaaacagtg ggtctgcgga tgtcgaaggc aacgaccagg
     1561 cgctcgccgt cgtcaaaggg gacggtcgaa tgccacaagg tcgacgggaa gacggcaagg
     1621 tgcccggcct gcggggccag ggtgcggtag ggggccaggt ccagccccag ttcgggcggc
     1681 ggcgcgccga aggcgatgtg cccggccggc gcggggccca tggcgaccgg atcgggcaag
     1741 gcgacgtaga acgcggtgga cagccagccc atcgcgtggg tgtgcggcac gttgtgcccc
     1801 agccggtgca gccggaccga ccagctgccc gcgatcagca agcgcccatc gcgcggggcg
     1861 gacagcagcg ggtgggccgg atcgttgggc ggcaggcctt cggaataatc gcggatcacg
     1921 gccagcagcc gggccttcag gcgctgcagt tcgggttcgt ggcgcagcag gaccgaacgg
     1981 tcggtctggg tgccgccgcg caccgactgt tcgatatagg gctggctggc ggtgtggaga
     2041 ccgcgcagca ggtcggccag cgcggccagt tcggccgggt cgatcccggc atcgaagctc
     2101 cggatcaggt ccgcgggccg gtccagccac tcggcccggg gatcccccag caggcgccag
     2161 cacagcgaga ggtagggcca gtaaagcgcc gccgaaggcc cggcggtcag cggcagggcc
     2221 tcgccctcgg ccgtgcgggc gtcgccctgg cgcagcgcgt ggcgaatccg gcacaggctg
     2281 gccacgtcgc cgcggatcgc cgcggttgcg gcaatgtgcc gcgcggcgtc gggcgcggcg
     2341 ctttccaccg cgacgaacag gcgcgagacg gcgagatccg gccgcgggcc gaggtgccgt
     2401 tcggcttcgt ccagcacggc gctggccgcc tgccactgcc gggcctgggc gaggttgcgg
     2461 aaccaggcga gccacagcgc cgcgttgtcc ggctgggcgc ggcaggccgc ggcgaggtga
     2521 tcggaaaagc cggtctggtc gccgccggtc cagttcagcg tcgccagcgc cttgtggccg
     2581 tccagccaat cgggatggcg cgccagccgc gcttccaacc gggcgcgggc cgcatcgcgg
     2641 cggccttcgg acagcagcgc cagggcgtag ttgcggacga tttcgggatg gtccggggcc
     2701 agggcctcgg cccgggcgaa caggtctgcc gcgggatagc cccgctcata gcggacctgg
     2761 gcatggacga aggccagacc cggatcgccg gggaaccggg ccacggcggc cgccagatcg
     2821 cgctcagcct cgtccagccg ctgctcggcg cagagcgcga gggcgcggga gcgcagggtc
     2881 tggggatcgg cggaatccat cggtctgtct tgcggcgggg cagggggcct gtcgagaggc
     2941 tgtgaacgga aaggggaggc gaccccgaaa gaccgcctcc ccccgtttcc gttcgcagcc
     3001 gggctgcgga tcagaagctg tacttgatcg aggccgtcca ggtccggccg agggccgggc
     3061 ccgtcccgat cacgcccggg ctgaccgaga gatcggccgg cccgccgctt ccgcgcaggt
     3121 cgtaacggtt gaagatgttg taaccgttga tccccagttc caggttcggg atcgggcgga
     3181 tcgagaagtc gagcccgatc accgcgccgc ccggatatcc ccggccgccg tcgtcgacga
     3241 tccccgactg gccggtggcc gaaacgccca gcgtcacgat gtcgctgaag tcatagctgg
     3301 cctgaacggt gtagatcagg tccggcatgt actgcgcacg ggcgtaaggc gtcggattgc
     3361 cgctgccgtc cacgcccgaa cgttcggcct tggtccaggt cgcgttgccg accaggctga
     3421 agccgccgct ccggtaggtg ccatagaact caagcccgta ggacttgtag cgcgcgtcga
     3481 tcacgcagcc gcccgagccg ccggggcagc gggtggccga cagttcgaag gtcgactgct
     3541 tgaagctgcc cttcagcagg gtcagttcca gggtgtagcg cccgccggcc atttcgccgc
     3601 ggttcttcag gcccagttcg tactgcttga cgaagtcgac cgagggggtg acgccgtcgg
     3661 cgacgcagcc catggtcccg gcggccgcca tggccccggt gcagagcgaa ccgtcgctgg
     3721 cgatcttgcc gccaaaggtc tggcggtcac cgttgaagcg gccgccgcgc gaagcccgca
     3781 cgaagacgct ggtgttgccg ttgaccttcc acagcgcacc ggcggtccac gaggtgtagc
     3841 tgcgcgagta gttcagcgtt tcgcgggtgc cgttgctggt gatcgcgggg atcaggacgc
     3901 cgttggagtt gacgaagaac tcgctgccgc cggccgtgcc ggtgcccgag gccttgacgt
     3961 tttcgaaccg gaccgaaccg tcgaggttga aatcgtcgct gtccagttcc agcgcggcat
     4021 agggcgccgt gttggtgtag gacaggtcat agtcacgggc gcagcagttg ccccagttgt
     4081 tgttgtaacc ggaaatgccg tcctgggtca gcttgttccc ggcaccatca tagaggtcca
     4141 gctgggccgg gttgttgccg ctcacttcgc gcagcgactt gttggtgtgc cagtccatcg
     4201 cgatcttctg gttcatgtag aagaagccgc cgcgcacggt cagcttcatc gaaccggcgt
     4261 cgaacttgcc cgccagagtc aggtcgttgg ccaggctgcc catgtcgcga atgttggtgt
     4321 ggacgttggt gttgttgtca acataggtgc cggtgaacac ctgcccggcg cgcggaccgt
     4381 tggcgtaacg gatcgaggca accgtggcat agggcgtccc gttgatcgtc aggttggagc
     4441 cgacgatgcc ggacgcgcgg ctggtgttct ggaacggcga ggtgaagctg ccgctcatct
     4501 tggtccagcg catgttgttg tcgacggtga tgttctcgcc gaactcatag tggaactggc
     4561 tgcccagcga ggtcgccttg gtgcggattc cgtccatgcc gacgcgctcc agctggcccg
     4621 agcggcccag gatcaggaac gactggttgt agatcgaata gttcgagctg ttgcggccat
     4681 cgaagcccgg gaagggctgg acattgctga acttgccgcc gctcagcgtg gcgagggccg
     4741 gggcgccggt gtagttcggt tccttggtgt cggcgacctt gaacagcagg cggaaatagc
     4801 cgcggccatc gggcaggtcc ttggtgatgt tgcccttcac ctggatcgaa tccgacacgt
     4861 tgtaatcggc gtgaagcggg ccgcggccgg tcttgaacga gccgccgatg tggaaccgca
     4921 ggtcttcggc cagggacgtg ccatagcgga agtcgatctt ggtttcgtcg tagttcacgc
     4981 ccttcgaaac gcggacgaag ccgccttcct tggcgccggt gttgctgatg tagttgatga
     5041 ccgcacccgg agcctgcgag gcataggtgg tggccccgcc gccgcggacc gattcgacgc
     5101 gctcgacgct ggcgtcaaag cgggtccagt agtcgttgtt gccgaactgg atgtcaccga
     5161 acagcacggt cggcaggcca tcttcctgga tctggacgaa gggcgaaccg ccggtggcaa
     5221 ccggcaggcc gcgcaccgcg atgttggagt tgccgcccgg gcccgaggtg ccgggcacct
     5281 ggatccccgg gatcatgcgg aacacttcgg cttccgacga ggggttcatg ccttcgatca
     5341 ggctggcatc gacgctggtc gaggagaccg agctgtcgag cttggtcttg tcgcggcccg
     5401 aagccgtgac gacgatttca tcgagaccgg tcgcttcggc ttcatcggcc ggcgcgtcct
     5461 gcgcaaaggc cggggccgca accagcgcca tggccgccac agcagccaca gacgcacaac
     5521 tccgcaatac ggatttcttc atgtcctctc ccttccaaca ggatgcgtgc ttgcatgacg
     5581 caagtcgagc ctgtcactgg catatgcaac aatcgattgc aatgggattc ctcaatcgat
     5641 tgcagaatac gtttgcatgt gtccggcagg ccacaatgcg ggccaccgcg ccgaggggaa
     5701 tatgaccggg atcaatgcgc gccgagcgct cggcggggca caggcattga cagccgctcc
     5761 gctcgcggcc catggaccat gcaaccgatt gccccggggg agcctttcgc aaaccgatgt
     5821 ccgaagacac gccgccaccg acccggattc gcaacattgc cgaactggcc cgcctggccg
     5881 gggtttcggc cggcacggtc tcgcgcgcgc tggcggacat gccgctggtc aacaaggaaa
     5941 cccgccgccg gatccaggcc ctggcgcgcg agcatgggtt ccgccccaac cagatggcgc
     6001 ggcggctgcg ggtgcaggaa accaaggtga tcggcgtggt cgtgccgctg gggcatgaac
     6061 agcgccagca cctgtccgat ccgttcttca tggcgatcct gggccacctg gccgacgccc
     6121 tgaccgagaa tggctatgac atcatgctca gccgcgtgat ccccgatgcc gaggactggc
     6181 tggaacggat cgtctcgtcg ggcatgctcg acggggtcct gctgatcggc cagtcgaacc
     6241 agttcgacgc gatcgagcgg gttgcgcagt ggtatcggcc gatggtcgtc tggggcagca
     6301 accgccccgg ccaggcccat tgcacggtcg gggtcgacaa ccgcaagggc ggccgcctgg
     6361 ccggagaatg cctgatcgcc tcgggccgca aacggctggc attcatgggc gagaagatcg
     6421 ccccggaact gtccgagcgc tatctgggcc tctgcgatgc cgcggctgcg gccgggctgc
     6481 cgccgccgct gcagctcgac acccacatgg cgagcgacgt gatggccgac gagattgccg
     6541 gccacatcgc ccggatcgcc gcggaagtgg acgggatcgc ggccgcatcg gacctgatcg
     6601 cgatgcacac cgtccgcgcg ctggctgacc gcgggatcgc ggtgccgggc cagatcgcgg
     6661 tgaccgggtt tgacgatctg ccgctggccg aacagaccat cccgcgcctg accacgatca
     6721 gccagaacgt ccccgcaggg gccaaggcca tggtcaaggc gctgtttgac cggatcgccg
     6781 ggatcgacgc cgcttcgctg gaaatggcac cggaactggt ccggcgcgac acggcctagg
     6841 ccgcgctggc ggcggccggc tcggcccgct ccaggcggta ctccgctccg ctcagccggt
     6901 gctgcacggt cagcgccgcg cagccgcgcc cggccagcac ttccaggctg ccgccgtcaa
     6961 tccagatttc gaccggatcg gcgggatcgg cttcggcctg ccagctgcca tcgaggaacg
     7021 gcgattgcgg gtcggtccgc tccaggacca gccgcgcgcc ttgccgggtg acctggaccg
     7081 cgcggccttc gccgtcccgg atcgccagcg caaagtcggc gccgcccggt tggataagcc
     7141 gcgcggcgaa cagggcctgt gccgcgctgg catccgccag cgggacttgc ggcaccagcg
     7201 cgggctccac ctcctgccgc aggaccagtt cctccccggt acggaccagc gagaggcggc
     7261 gcggcgtgct catcgccccg cgccagccct ggcggggcag ctggccctga taggcgtggt
     7321 tgccgatcca gccgatccag gccggccggc cggcggcatc gcgcggttcg tgccaggcga
     7381 tcgcggcata gaaatcgttg ccccagtcgg ccacgcgcca gtcctcgttc gcatcgctga
     7441 caaagcgggt gccatcgaaa tggccggtcc ggtacagcgc ccccgatccc ggcgcgccgc
     7501 gcagcacgtc gaccttgaac agccagcggg tgcccgtttc gccttcgacc ggcagttc
//
LOCUS       DASUDV010000005         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2076635,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000005 DASUDV010000000
VERSION     DASUDV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076635"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFV30_00080"
     CDS             complement(<1..373)
                     /locus_tag="VFV30_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate-protein ligase B"
                     /protein_id="HEX4846511.1"
                     /translation="MDTSLPDTIEWRRSAEQVPYRLALDEMTLRNAAIAEGSAAELVW
                     LLEHPPVYTAGTSAAVGELLDPRFEVVEAGRGGRYTYHGPGQRVGYVLLDLKRRARDV
                     RGFVHALEGWVIDTLGDFGVES"
     gene            440..1255
                     /locus_tag="VFV30_00085"
     CDS             440..1255
                     /locus_tag="VFV30_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846512.1"
                     /translation="MRFDSSRAWSEASQAVSANREVLLALAGVFLVLPTFALGVFAPQ
                     PEAPAGASPEQLLALLGEFYSAHWPKFVAAGLLSVVGTMAMLALFTDARRPTVGEAIK
                     LGAIGAISVIAAQILMGMAIGALIVIPAAVAGAIGVPILPILVLIAACAVSVWLAIRL
                     SMLSPAVMVEGIRNPVAALQRSWQLTRGNAGRLLVFFALLVIVFVVVMLLLSAALGAV
                     LALALPQDGAAIGGALMGSILQAIFNVYMIGVVAASHRQLAGPTASVAASPFE"
     gene            complement(1271..1879)
                     /locus_tag="VFV30_00090"
     CDS             complement(1271..1879)
                     /locus_tag="VFV30_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4846513.1"
                     /translation="MRPVEQLSIVRKVIRALGPWRVFLQGFIEHPVMVGSIIPSSRFT
                     IRRMLAPVRWDECRLFVEYGPGVGTFCRPVLERLREDGRLIAIDTNPLYIDYLNQAIS
                     DSRFTAVLGSAADVETIVRAHGHDKADYVLSGLPFSTLPDGVGPAIAAATHRVLRPGG
                     AFLVYQFSARARDYMARHFARIDAGFEWLNVLPCKLFWGWKD"
     gene            1994..>2268
                     /locus_tag="VFV30_00095"
     CDS             1994..>2268
                     /locus_tag="VFV30_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4846514.1"
                     /translation="MSRLATPQLEEPAKTTASLPRGRVALLFAVMLVTAAGNTAMQSV
                     MPSIGTALGVADVWISLAYTWSALLWMTCAPMWARRSDRRGRKAMMA"
BASE COUNT          406 a          720 c          733 g          409 t
ORIGIN      
        1 agctttcgac accgaaatcg cccagcgtat cgatcaccca gccttccagg gcatggacga
       61 acccgcgcac atcgcgggcg cgccgcttga ggtcgagcag gacgtagcca acccgctggc
      121 ccggaccatg ataggtataa cgcccgccgc gcccggcctc gaccacttcg aagcgcggat
      181 cgagcagttc gccgacagcc gcgctggtgc cggcggtgta gaccggggga tgctccagca
      241 gccagaccaa ctctgccgca ctgccttcgg cgatcgcggc attgcgcaag gtcatctcgt
      301 caagcgcaag ccgatagggc acctgttcgg ccgaacggcg ccattcgatc gtatctggca
      361 ggctggtatc catcaatgaa ttgcgtggcg ccaaatgctg tggttatcaa ggggcaagaa
      421 gcaaccaagg ggaagcatga tgcggtttga cagcagcagg gcctggagtg aggcgtcgca
      481 ggccgtatcg gccaaccgcg aagttctgct ggcgctggcc ggggtgttcc tggtcctgcc
      541 aacctttgcc ctgggcgtct tcgcgccgca gcccgaagca cccgcaggtg ccagtcccga
      601 acaactgctg gccctgctgg gcgaattcta ttcggcccac tggccaaagt ttgtggcggc
      661 tggactgctc agtgtggtcg gcaccatggc catgcttgct ctgtttaccg atgcccgccg
      721 cccgaccgtg ggcgaagcca tcaagctggg cgcgatcggc gcgatcagcg tgatcgccgc
      781 ccagatcctg atgggcatgg cgatcggcgc gctgatcgtc attccggcgg cggtggccgg
      841 cgcaatcggg gtgccgatcc tgccgatcct ggtgctgatc gcagcctgcg cggtatcggt
      901 ctggctggcg atccgccttt cgatgctgag cccggcggtg atggtcgaag ggatccgcaa
      961 cccggttgcc gccttgcagc gttcgtggca gttgacccgg ggaaatgccg ggcggctgct
     1021 ggttttcttc gcattgctgg tgattgtctt cgtcgttgtg atgctgctgc tttctgccgc
     1081 gctcggcgcg gttctggcgc tggccttgcc gcaggacggc gcggcaatcg gcggggcgct
     1141 gatgggcagc atcctccagg caatcttcaa cgtctacatg atcggcgttg ttgccgcctc
     1201 ccaccgccaa ctcgccggac ctaccgccag cgtcgcggca tcgccgtttg aataggccgg
     1261 tgatgcgggt ttagtccttc cagccccaga acagcttgca cggcaacacg ttcagccatt
     1321 cgaaaccggc atcgatccgg gcgaagtgcc gggccatgta atcgcgcgcc cgggcgctga
     1381 actggtagac cagaaaggcc ccgcccggcc gcaggacccg gtgcgttgcc gcagcaatcg
     1441 ccggaccgac accatccggc aaggtcgaaa acggcaggcc tgacagcaca tagtcggcct
     1501 tgtcgtggcc atgggcgcgc acgattgttt caacatcggc ggctgagcca agcaccgcag
     1561 tgaaccgcga gtccgaaatc gcctggttga ggtaatcaat atagagcggg ttggtatcga
     1621 tcgcgatcag tctgccatcc tcgcgaagcc gctcaagcac cgggcggcag aacgtgccga
     1681 cccctgggcc atattccacg aatagcctgc attcgtccca tctgaccggg gccagcatcc
     1741 gccggatcgt gaaacgcgag ctgggaatga tcgacccaac catcactgga tgctcgatga
     1801 acccctgaag aaacaccctc cacgggccga gcgcccggat cactttccgg acgatcgaga
     1861 gctgttcgac gggacgcata aaatcaggtc tgctcatttt gaaaattagt cgcgctgctg
     1921 aaaggaggag gcgcccatca caaatctgcc gcgccattgc aagcggcgaa cgagacgact
     1981 agcgagattg gcaatgtccc gcctcgccac cccgcagttg gaagaaccgg ccaagacaac
     2041 cgcgagcctg ccgcgcggcc gcgtggcgct gctgttcgcg gtgatgctgg ttacggcggc
     2101 gggcaatacc gcgatgcaat cggtcatgcc ttcgatcggc accgcgctgg gggtggccga
     2161 tgtctggatc agtctggcct atacctggtc ggcgctgttg tggatgacct gcgcgccgat
     2221 gtgggcgcgg cgcagcgatc ggcgcggccg caaggcgatg atggcgat
//
LOCUS       DASUDV010000006         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_441882,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000006 DASUDV010000000
VERSION     DASUDV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441882"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFV30_00100"
     CDS             complement(<1..1080)
                     /locus_tag="VFV30_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX4846515.1"
                     /translation="MAHTVRKAGIVGGGVIGAGWAARFVLNGIDVAIFDPDPEIERKT
                     HEVIANARRALTRLFGQALPAEGTVTIAATLADAVRDADFIQESLPEREELKQKVLAE
                     VDAAMPAHAIIGSSTSGLLPTKLQSGMARPDRLVVGHPFNPVYLLPLVEVCGGEQTSA
                     ETIAAAAAVYERIGMKVLHVRKEIDGFIADRLLEAVWREALWLINDGVATASEIDDAI
                     RYGAGMRWSFMGTFLVYRLAGGEAGMRHFLHQFGPSMEWPWTKLIGPKLTDELVDTIA
                     RQSDEQAGNVDLRTYERLRDDCLVDLTHALERNDFAAGAVAKAHRERLGAAPGADGSA
                     DENGRLRTIATPVQPEWIDYNDHLTE"
     gene            complement(1092..1982)
                     /locus_tag="VFV30_00105"
     CDS             complement(1092..1982)
                     /locus_tag="VFV30_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX4846516.1"
                     /translation="MNFEPFITCAVTGSGNTQGIHPDLPITPEQIANAAIEAARAGAA
                     IAHIHVRDPETGKGCRDVALYREVKERIRASDVDVIINFTAGMGGDIVFGPGETPLPL
                     DPAGTDMVGPLERLLHVQELLPEICTLDCGTMNFAEADYVMTNTPGMLRTMAAEVQKL
                     GVRPELEVFDTGHLVFVKELIRDGLIDDPVMIQLCLGIPYGAPDDPLTTMAMVHQMPA
                     GAVFSGFSISRNQLPFVAMAALAGGNVRVGLEDNLYLAKGQFASNGQLVERAVQVLTG
                     MGARILGPAEVRAKLKLTKR"
     gene            2087..3034
                     /locus_tag="VFV30_00110"
     CDS             2087..3034
                     /locus_tag="VFV30_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4846517.1"
                     /translation="MQTMAFLLVPGFSAMAFFAALEPLRIANRLAARPLYRWSIHAPA
                     GDHALASNGMQLRTDGPLPDRPEALIVCAGFDPLLAAGPGLLGQVRRLWRAGCQVGAI
                     DTGAFVLAEAGILGRETITLHWEAAEEFRRRYPRIPVSTELYERHARLFTCAGGTAAM
                     DMMLDTIAHSHGIALASAVSQQLIHDAIRPPSAPQRATRAEQAGAAGAVVQRLIAAME
                     RRLGEGAALEAILADEGISRRQAERLFRARLGTSPGHFLRDLRLRHAIGLMRDARMPV
                     HEAALAAGFSSQPVFSRACRQHFGQSPRELVSGPGLVEA"
     gene            complement(3008..>3682)
                     /locus_tag="VFV30_00115"
     CDS             complement(3008..>3682)
                     /locus_tag="VFV30_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX4846518.1"
                     /translation="EALADRAGKLAYMAALDRPHIRVAGSLPPLFGSYRPDLFDAAAA
                     PARIAPLIAGLAPHVDLWLAETLSAIEEAQVVANALADRDKPLWLSFTLEDEAADAAT
                     APRLRSGEPVALAARFAQECGAEALLFNCSQPEVMEAALRAAQAEAPGLTLGVYANAF
                     PPQPKDASANETLHDLRADLDPEGYLGWVRRWVECGARIVGGCCGIGPDHIAAIAASG
                     LDQPRP"
BASE COUNT          532 a         1276 c         1293 g          581 t
ORIGIN      
        1 ttcggtcagg tgatcgttgt agtcgatcca ctccggctgg accggggtgg cgatggtccg
       61 caggcggccg ttttcgtccg cgctgccatc ggcgcccggc gctgcgccca gccgctcgcg
      121 gtgggccttg gcgacggcgc ctgcggcgaa atcgttgcgt tccagcgcgt gggtcaggtc
      181 gaccaggcaa tcgtcgcgca gccgttcata ggtgcgcagg tcgacattac cggcctgctc
      241 gtcggactgg cgggcgatgg tgtcgaccag ctcgtcggtc agcttggggc cgatcagctt
      301 ggtccagggc cattccatcg aggggccgaa ctggtggagg aagtggcgca tcccggcttc
      361 gccgccggcc agacggtaga ccaggaaggt gcccatgaaa gaccagcgca tcccggcgcc
      421 atagcggatc gcgtcgtcga tctcgctggc cgtggcgacc ccgtcgttga tcagccacag
      481 cgcctcgcgc cagaccgctt ccagcagccg gtcggcaatg aagccgtcga tctccttgcg
      541 gacgtgcagc actttcatgc cgatccgctc gtaaaccgcc gcggccgccg cgatcgtttc
      601 cgcgctggtc tgttccccgc cgcagacctc gaccagcggc agcaggtaga ccgggttgaa
      661 cggatggccg accaccagcc ggtccggccg ggccatgccg gattgcagct tggtgggcag
      721 caggccggaa gtggacgagc cgatgatcgc gtgggccggc atggccgcat cgacctcggc
      781 cagcaccttc tgcttcagct cttcgcgctc gggcaggctt tcctggatga agtcggcatc
      841 gcgcacggca tcggccagag tggcggcgat ggtcaccgtg ccttcggcgg gcagggcctg
      901 gccgaacagc cgggtcagcg cccggcgggc gttggcgatc acctcgtggg tcttgcgctc
      961 gatctcggga tcgggatcga agatcgccac gtcgatgccg ttgaggacga agcgcgcggc
     1021 ccagccggcc ccgatcaccc cgccgccgac aatcccggcc ttgcgaaccg tgtgcgccat
     1081 gcccgtcctc ctcagcgctt ggtgagcttg agcttggcgc gcacctcggc cgggccgagg
     1141 atccgcgcgc ccatgccggt cagcacttgc accgcgcgct caaccagctg gccgttggag
     1201 gcaaactggc cctttgcgag atagaggttg tcctccagcc cgacgcggac gttgccgccg
     1261 gccagcgccg ccatggccac gaagggcaac tggttgcggc tgatcgagaa cccgctgaac
     1321 accgcgcccg cgggcatctg gtggaccatg gccatcgtgg tcagcggatc gtccggtgcg
     1381 ccatagggaa tgcccaggca cagctggatc atcaccggat cgtcgatcag cccatcgcgg
     1441 atcagctcct tcacgaagac cagatggccc gtgtcgaaca cttccagctc cgggcgcacg
     1501 cccagcttct gcacttcggc cgccatggtc cgcagcatgc cgggggtgtt ggtcatcacg
     1561 taatcggctt cggcaaagtt catcgtgccg cagtcgagcg tgcagatctc gggcagcagt
     1621 tcctgcacgt ggagcagccg ctccagcggg ccgaccatgt cggtcccggc gggatcgagg
     1681 ggcagggggg tctcgcccgg gccgaacacg atgtcgccgc ccatcccggc ggtgaagttg
     1741 atgatcacgt cgacatcgct ggcgcggatc cgctccttca cttcgcggta aagcgccacg
     1801 tcgcggcagc ccttgccggt ctcgggatcg cgcacgtgga tgtgggcaat cgccgccccg
     1861 gcccgcgccg cctcgatcgc ggcgttggcg atctgctcgg gggtgatcgg caggtccggg
     1921 tgaatgccct gggtgttgcc cgatccggta acggcgcacg tgatgaacgg ttcaaagttc
     1981 atggttctct cccttgccgc cagcttaggg cgcggcgccg ccttgcaaat cgccgtttgc
     2041 ggcaaagcat tgcaaaatgc gacaagggcg cgcagggtac cgtgcgatgc agaccatggc
     2101 ctttctgctg gttccgggct tttcggcgat ggcgttcttc gccgcgctgg aaccgctgcg
     2161 gatcgccaac cggctggcgg cgcgtccgct ctaccgctgg tcgatccacg cgcccgcggg
     2221 cgaccacgcg ctggcctcga acgggatgca gctgcgcacc gacggcccct tgccggaccg
     2281 gcccgaggcg ctgatcgtct gcgccgggtt cgatcccctg ctggcggccg ggccgggact
     2341 gctgggccag gtccggcggc tgtggcgcgc cgggtgccag gtcggcgcga tcgataccgg
     2401 cgccttcgtg ctggccgaag ccgggatcct ggggcgcgag acgatcacgc tgcactggga
     2461 agcggccgag gaattccgcc gccgctatcc tcgcatcccg gtttcgaccg agctctacga
     2521 acgccacgcc cggctgttca cctgcgcggg cgggaccgcg gcgatggaca tgatgctcga
     2581 tacgatcgcc cacagccacg ggatcgcctt ggcaagcgcg gtttcgcagc agctgatcca
     2641 cgatgcgatc cgcccgccca gcgccccgca gcgcgccacc cgggcggagc aggccggcgc
     2701 ggccggggcg gtggtccagc ggctcatcgc ggcgatggag cgccgcctgg gcgagggcgc
     2761 cgcgctggag gcgatcctgg cggatgaagg gatcagccgc cgccaggccg agcgcttgtt
     2821 ccgcgcccgg ctgggcacca gccccgggca tttcctgcgc gaccttcgcc tgcgccacgc
     2881 gatcggcctg atgcgggatg cgcggatgcc ggtgcacgaa gcggcgttgg cagcggggtt
     2941 ctcgtcgcag ccggtgttct cgcgcgcgtg ccggcagcac ttcgggcaaa gcccgcgcga
     3001 gctggtctca gggccggggc tggtcgaggc ctgacgcggc gatggccgca atgtggtccg
     3061 ggccgatccc gcagcatcca cccacgattc gggccccaca ctcgacccag cggcgcaccc
     3121 agccgaggta gccctcggga tcgagatcgg cgcgcagatc gtgcagcgtc tcgttggccg
     3181 aggcatcctt gggctgcggg ggaaaggcgt tggcatagac gcccagggtc aggccagggg
     3241 cctctgcttg cgcggcccgc agcgccgctt ccatcacttc gggctggctg cagttgaaca
     3301 gcagcgcctc tgcgccgcac tcctgcgcaa agcgggcagc cagcgccacc ggctcgcccg
     3361 agcgcagacg cggcgcggtg gcagcgtccg cagcctcgtc ctccagcgtg aaggacagcc
     3421 acagcggctt gtcccggtcg gccagagcat tagccaccac ttgcgcttcc tcgatcgcgg
     3481 agagggtttc ggcgagccac agatcgacat gcggggccag cccggcaatc agcggggcaa
     3541 tccgcgccgg cgcagcggcg gcatcgaaca ggtccggccg gtaggagccg aacagcggcg
     3601 gcaggctgcc cgccacgcga atatgcggcc ggtcgagcgc ggccatatag gccagcttac
     3661 ccgcccggtc ggccagggct tc
//
LOCUS       DASUDV010000007         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_331465,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000007 DASUDV010000000
VERSION     DASUDV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331465"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2140)
                     /locus_tag="VFV30_00120"
     CDS             complement(212..2140)
                     /locus_tag="VFV30_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX4846519.1"
                     /translation="MIQSLLIANRGEIACRIIRTARSLGIRTVAVYSDADAKALHVRQ
                     ADEAVHIGPSPARESYLVGDKIIAAAKATGAEAIHPGYGFLSENAEFAQAVIDAGLVW
                     VGPKPASIEAMGLKDAAKKLMLEAGVPCTPGYLGENQDPAFLASQAAEIGYPVLIKAV
                     AGGGGKGMRLVEQAEDFADALASCQREASASFGNAHVLIEKYILSPRHIEVQVFGDSH
                     GNVVHLFERDCSLQRRHQKVIEEAPAPGMDAATREAVCAAAVRAAKAVNYEGAGTIEF
                     IADGSEGLRADRIWFMEMNTRLQVEHPVTEEITGVDLVEWQLRVASGEPIPLRQDELS
                     VNGWAMEARLYAEDPAKGFLPSIGRLEHLRIHLGLPEYDMPDRRRVRIETGVEQGDTI
                     SPFYDPMIAKIVVHACSRAKAIDLLATACNDTQVWPVKTNANFLVRLCEDGGFSGGAM
                     TTGLIAERGESLVAPEPVAAPEIFEAARWLGSAFPAAPIAPAFRLNREAAQTVTVQID
                     GQAVPVEYSPFMAGLMTQLGGLDAINGHSPLEGDGGSGIVFSRGRALTVSLGVRGTGH
                     HSAHDGDILSPMPGKIIAVEVSEGQAVTKGQKLLTLEAMKMEHTLTAPFDGTVAELSA
                     TPGAQVQVEALLARIEAA"
BASE COUNT          391 a          873 c          813 g          405 t
ORIGIN      
        1 agcattccgc cggggtgcgg gtggcgatcc gggtttcggc cagcaccgtg ccatcggccc
       61 gcgccagggc gaggacgaac ttggtcccgc ccgcttcgat cgcgccgatc aggcggctat
      121 cctgtggggt atcctgcatg gcccaagtgc tcgcacggcg cgccagcccc ggcaatcctt
      181 tgcatacgaa ttttgcgcgc ggacggcgcg gtcaggccgc ttcgatccgc gccagcagcg
      241 cctcaacctg cacctgcgcg ccgggggtgg cggaaagctc cgccaccgtg ccgtcgaacg
      301 gcgcggtcag ggtgtgctcc atcttcatgg cttcgagggt gaggagcttc tgccccttgg
      361 tcaccgcctg cccctcgctc acttccaccg cgatgatctt gcccggcatg ggcgagagga
      421 tatcgccgtc gtgggcggag tggtggccgg tgccacgcac accgagcgag accgtgagcg
      481 cccgcccgcg cgagaacacg atcccgctgc cgccgtcgcc ctccagcggg gaatggccgt
      541 tgatcgcatc cagaccgccc agctgtgtca tcagcccggc catgaagggg gagtattcca
      601 ccgggaccgc ctgaccatcg atttgcaccg tcaccgtctg cgccgcttcg cggtttaggc
      661 ggaacgccgg ggcaatcgga gcggccggaa aggcggagcc aagccagcgg gcggcctcaa
      721 aaatctccgg tgcggccacc ggctcggggg cgaccaggct ttcgccgcgc tcggcgatca
      781 ggccggtggt catcgctccg ccgctgaaac cgccgtcctc gcacagccgg accaggaaat
      841 tggcattggt cttgaccggc cagacctggg tgtcgttgca cgccgtcgcc agcagatcga
      901 tagccttcgc gcggctgcag gcgtggacca cgatcttggc gatcatcgga tcgtagaacg
      961 ggctgatcgt atcaccctgc tccaccccgg tctcgatccg gacccgccgg cggtcgggca
     1021 tatcgtattc gggcaggccg aggtgaatgc gcagatgctc cagccggcca atactcggca
     1081 agaacccctt cgcgggatct tccgcataga gccgcgcttc catcgcccag ccgttgacgc
     1141 tcagctcgtc ctgccgcagc gggatcggct cgccgctggc cacccgcagt tgccattcga
     1201 ccaggtcgac gccggtgatt tcctcggtca ccgggtgttc cacctgcagg cgggtgttca
     1261 tttccatgaa ccagatccgg tcggcgcgca ggccctcgct gccgtcggcg atgaattcga
     1321 tcgtgccggc gccttcgtag ttcaccgcct tggccgcgcg gacggcggcg gcgcagacgg
     1381 cctcgcgggt cgccgcgtcc atccccggcg cgggggcttc ctcgatcacc ttctggtgcc
     1441 ggcgctggag cgagcagtcg cgctcgaaca ggtggacgac attgccgtgg ctgtcgccga
     1501 acacctgcac ttcgatgtgg cggggggaga ggatgtactt ctcgatcagc acgtgcgcat
     1561 tgccaaagct ggcgctggcc tcgcgctggc agctggcaag ggcgtcggca aagtcctcgg
     1621 cctgctcaac cagccgcatc cccttgccgc cgccgccggc cacggccttg atcagcaccg
     1681 gatagccgat ttcggccgcc tggctggcca ggaaggccgg gtcctggttc tcgcccagat
     1741 agcccggcgt gcagggcacc ccggcctcca gcatcagctt cttggccgcg tccttcagcc
     1801 ccatcgcctc gatggaggcg ggcttgggcc cgacccagac cagacccgca tcgatcacgg
     1861 cctgcgcgaa ctccgcgttt tccgacagga acccatagcc cggatggatc gcctccgcgc
     1921 cggtggcctt ggcggcggca atgatcttgt cccccaccag atagctctcg cgcgcgggcg
     1981 aagggccgat gtgcaccgcc tcgtccgcct ggcgcacatg cagcgccttg gcatcggcat
     2041 cggaatagac cgccacggtg cgaatgccga ggctgcgcgc cgtgcggatg atccggcaag
     2101 cgatttcgcc gcggttggcg atgaggaggg attggatcat aagtctatca ccaggctgtc
     2161 gatcagcagg gccgccgcaa gcaagccaag cacagaggta ttgaaccagt acagaaacgg
     2221 gttccgatcg cgtgtgaacg gcacggtggg gctggcgctc ataactgcgc ccgaacgcag
     2281 cgcccggacg gcccagatcg ccaggatcgt cccaaccaga atggcaagag ggttcaggct
     2341 tccactcaca tccggaacac cccaaaggcc ggccggtccg ggatcggagc ctccagcgcc
     2401 gccgccaggg ccagccccag cacatcgcgg gtctgggccg ggtcgatcac cccgtcgtcc
     2461 cacagccgcg ccgtcgcgta at
//
LOCUS       DASUDV010000008        10288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2098711,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000008 DASUDV010000000
VERSION     DASUDV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098711"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="tgt"
                     /locus_tag="VFV30_00125"
     CDS             <1..1126
                     /gene="tgt"
                     /locus_tag="VFV30_00125"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX4846520.1"
                     /translation="APRFSFSVSATDGKARTGTIRMRRGEIRTPAFMPVGTAATVKAM
                     KPESVRATGADILLGNTYHLMLRPGAERVARLGGLHKFMNWDRPILTDSGGYQVMSLS
                     ELRKITEEGVTFKSHLDGSKHLITPERSMEIQRLLGSDIVMAFDECPRADAPLDVIAA
                     SMRMSMRWAARSRAGFDSGEEHAERSALFGIQQGALNEDLRRESAEALQDIGFDGYAI
                     GGLAVGEGQEAMFATLDFAPGMLPVDKPRYLMGVGKPDDLVGAVERGVDMFDCVLPSR
                     SGRNGQGFTWNGPVNLRNAKHAEDPAPLDERCPGDCCTKYSRAYLHHLHKSGEILGAM
                     LLTEHNLTFYQQLMAAMREAISAGRFAQFAVQFRRDYLRT"
     gene            complement(1123..1290)
                     /locus_tag="VFV30_00130"
     CDS             complement(1123..1290)
                     /locus_tag="VFV30_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846521.1"
                     /translation="MKLFRFVTPHRVGKWYPDLNLARQQAAAIGAGFLERRTGMFVAY
                     RETRLETRRRD"
     gene            1506..3371
                     /locus_tag="VFV30_00135"
     CDS             1506..3371
                     /locus_tag="VFV30_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX4846522.1"
                     /translation="MFTINSVTVRLGGRTILDGASASIPPGGRIGLIGRNGAGKSTLM
                     KVLVGQLDPDEGSVELPKRTRLGYIAQEAPSGETTPFEAVLAADTERAALLEEAEHCV
                     DPNRLGDLHERLLAIDAYSAPSRAAIILTGLGFDEAMQAQPLDSFSGGWKMRVALAAL
                     LFSAPDVLLLDEPSNHLDLESTLWLESFLKSYPGTLLVISHERDLLNNVVDSILHLQG
                     GKLALYPGNYDSFEAQRAERAAQLAAAKASQDAQRARLQDYVARNSARASTAKQAQSR
                     AKMLAKMQPIAALMEDPSLSFDFPSPKEMRPPLITLDLAAVGYNDKPILQRLNLRIDP
                     DDRIALLGRNGNGKTTLARLLAAQLAPLDGVMRASGQMTVGYFTQYQVEELAGGDTPL
                     EHMTRAMPGAAPGAVRAQLGRFGFSGNRATTKVGSLSGGERARLALALITRDAPNLLI
                     LDEPTNHLDVDAREALVQALNEFDGAVILISHDRHMVELTADRLVLVDNGTASDYAGS
                     MEDYIDFVLGRNQPKADARPKPEKKDRKAAAEAREQARLARKDVSEAEAAIAKLQKQI
                     SAIDQAMFAPDSAAADLRKLTMGELSRRRGLVEKDLAVAEQQWLEASERLEQLAA"
     gene            complement(3379..4050)
                     /locus_tag="VFV30_00140"
     CDS             complement(3379..4050)
                     /locus_tag="VFV30_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX4846523.1"
                     /translation="MEYDEVVLGRRSIRGYLDKPVPRELIEEVLTLAMRAPSSMNTQP
                     WHFHVITGEPLDRIRAGNTERNLAGVPHSREFRTGHAFEGVHRERQIAVAKQLFAAQG
                     IARDDKDARLDWVLRGFRQFDAPVCVIVTYDKELADSDDTAFDCGAVTTALVNAAWSR
                     GLGCVINSQGIMQSPVVREHAGIPDDQVIMKAVALGWPDETFPANAVVSERKSVDEAA
                     RFVGF"
     gene            4301..6283
                     /locus_tag="VFV30_00145"
     CDS             4301..6283
                     /locus_tag="VFV30_00145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4846524.1"
                     /translation="MAALLSTSGLAAAAPPSAESLALANKFGALESVQQMSLSPDGTK
                     IAFVTPLGGTSAVLKVADLSSGSAVPKSILLQRNEGETLRGCRWATDTRLVCRVSTII
                     NWGGYLLPFTRLFAVSSDGSDFKMLTGGVKTSSLGVVTFGGSVIDWDLPGKPGRVLVM
                     RQYVPDENARVISKLAEEGIGVEEIDVTTLERTNIERPTDETVEFISDGHGTVRIMGR
                     QPRNPAGYTKGTRFYYYRKQGSREWEDLSKIIYDQDAVGRGFDPYAIEYQRNAVLGFD
                     DNNGFRALYRIALDGSLTKELVLSRSDVDIDSVIQIGRDSRVVGASYATERRTAEYFD
                     PELKKLSAALSRALPGRPVVDFVDASINEQKLLVFASSDVDPGTFYRYDKATKRLEPI
                     LPIREQLAGMAMSEMKPVMIPAADGTQVPGYLTLPVGSTGKNLPAIVMPHGGPGSRDE
                     WGFDWLAQYFAARGYAVLQPNFRGSAGYGAAWYQKNGFQSWRTAIGDVNDAGRWLVSQ
                     GIAAPDKLGILGWSYGGYAALQSGVLDPGLFKAVVAIAPVTDLGRLKADAADYSNAPL
                     VNRFVGNGPHIREGSPAQNAEAFTVPVLMFHGSKDLNVSIGQSRLMATRLRGARKEVN
                     LVEFPGLDHQLGDGAARARMLSESDAFLRQAFRMAP"
     gene            complement(6347..6961)
                     /gene="rpsD"
                     /locus_tag="VFV30_00150"
     CDS             complement(6347..6961)
                     /gene="rpsD"
                     /locus_tag="VFV30_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX4846525.1"
                     /translation="MSKRQSSKYKIDRRMGENIWGRPKSSVNRRSYGPGQHGQRRKGK
                     LSDFGIQLRAKQKLKGYYGDVTEKQFKRTYQEASRMKGDTGQNLIGLLEQRLDMVVYR
                     CKFAPTIFSARQIVSHGHIYVNGVKCNIASRRVRPGDVVSLGKKAKEMALIAEAQGLP
                     EREIPDYVAPDGTDKATFVRVPTLDEVPYPVKMEPNLVVEFYSR"
     gene            complement(7122..7388)
                     /locus_tag="VFV30_00155"
     CDS             complement(7122..7388)
                     /locus_tag="VFV30_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846526.1"
                     /translation="MRLLLIAGAAALAMPTLAVAEEAPKTEEAAKPAKVRKLCRETQP
                     RTGSHRPGKRVCRTAEEWKAFDQAEAGYDGQTTTRSTGVTESKD"
     gene            complement(7443..8180)
                     /locus_tag="VFV30_00160"
     CDS             complement(7443..8180)
                     /locus_tag="VFV30_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX4846527.1"
                     /translation="MPASPEPARPVIVLVRPQLGENIGKAARAMLNFGLTELRLVAPR
                     DGWPNPSAGPAAAGADVVLEKAEVFETLAEAVADCAHVYATTVRKRGVTKPVLTPEEA
                     ARAVHTEPGRSAYVFGPERSGLETDDVALARAIVTVPINPEFGSLNLAQAVILLAYEW
                     SKNANLAQPTVEEILPPAPQEELEGMITQLEDLLQPTGYFRPESRAAATRRTLRNMLT
                     KPGWNHLEVRTMRGVLSALSKGRRELP"
     gene            complement(8164..8625)
                     /gene="nrdR"
                     /locus_tag="VFV30_00165"
     CDS             complement(8164..8625)
                     /gene="nrdR"
                     /locus_tag="VFV30_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX4846528.1"
                     /translation="MRCPFCAHDDSQVKDSRPTEDNTAIRRRRQCDSCGARFTTFERV
                     QLREITVVKSDGTRESFDRSKIDQSVALACRKRGIAQERMDQLVSGVQRQIETMGESE
                     VPAKAIGEMVMEGLRGLDTVAYIRFASVYRDFTEAKDFEDFASTVRDAGKS"
     gene            complement(8626..8826)
                     /locus_tag="VFV30_00170"
     CDS             complement(8626..8826)
                     /locus_tag="VFV30_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846529.1"
                     /translation="MSDNFVDDAKAEISGMAKDGLRHPSTKPVLTGAAVGAIAGAVLP
                     LVAWPTGLILGATFMFYKRVRP"
     gene            complement(8830..10140)
                     /gene="glyA"
                     /locus_tag="VFV30_00175"
     CDS             complement(8830..10140)
                     /gene="glyA"
                     /locus_tag="VFV30_00175"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX4846530.1"
                     /translation="MTTTLSAAPVRSAGFFTAHLAEADPEVHAAIQSELKRQRHNIEL
                     IASENITSLAVLEATGSVFTNKYAEGYPGKRYYGGCEYADVVENLAIERAKALFGCQF
                     ANVQPNSGSQMNQAVFLALLQPGDSFMGLDLNCGGHLTHGSPVNMSGKWFKPIPYGVR
                     REDELIDMDEVARIARENKPKLIICGGTAYSRHWDFKRFREIADEVGAWLLADISHFS
                     GLVAGGAHPSPFPHAHVVTTTTHKSLRGPRSGVILTNDEDLAKKLNSAVFPGLQGGPL
                     VHVIAAKAVAFGDALKPEFKAYAAQIVANAKALAASVTDQGLRVVSGGTDNHLMLVDL
                     SAKDVTGKAAEKGLDRAWLTCNKNGVPYDTRSPFVTSGIRLGTPAGTTRGFGEEEFRL
                     VGQLIAEVVDGMARNGDEGDGQVEQRVRERVEELCTRFPIYPGL"
     gene            complement(10155..>10288)
                     /locus_tag="VFV30_00180"
     CDS             complement(10155..>10288)
                     /locus_tag="VFV30_00180"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX4846531.1"
                     /translation="VIAMGARLTGVDMAKACLDAFLSTDFGGGRHAGRVEKLSNPSC"
BASE COUNT         1687 a         3325 c         3526 g         1750 t
ORIGIN      
        1 cgcgccgcgt ttcagcttct ccgtttccgc caccgatggc aaggcccgca ccggcacgat
       61 ccggatgcgc cggggcgaga tccgcacgcc ggccttcatg ccggtcggca cggcggcgac
      121 ggtcaaggcg atgaagccgg agagcgtgcg ggcgaccgga gccgacatcc tgctgggcaa
      181 cacctatcac ctgatgctgc gtccgggggc cgagcgggtc gcgcggctgg gcgggctcca
      241 caagttcatg aactgggacc gcccgatcct gaccgacagc ggcgggtacc aggtgatgag
      301 cctgtccgag ctgcgcaaga tcaccgagga gggggtgacc ttcaaaagcc acctcgacgg
      361 cagcaagcac ctgatcacgc ccgaacgctc gatggaaatc cagcggctgc tgggcagcga
      421 tattgtcatg gcctttgacg aatgcccgcg ggccgatgcc ccgcttgacg tgatcgccgc
      481 ctcgatgcgg atgagcatgc gctgggcggc gcgcagccgc gccgggttcg acagcggcga
      541 ggagcatgcc gaacgctccg ccctgttcgg catccagcag ggcgctctca atgaggacct
      601 gcgccgcgag agcgccgagg cgctgcagga catcggcttt gacggctatg ccatcggcgg
      661 gctggcggtg ggcgaggggc aggaggcgat gttcgccacg ctcgatttcg cgcccggcat
      721 gctcccggtg gacaagccgc gctacctgat gggggtgggc aagcccgacg atctggtcgg
      781 cgcggttgaa cgcggggtcg atatgttcga ttgcgtcctg cccagccgct cgggccgcaa
      841 cgggcagggc ttcacctgga acggcccggt caacctgcgc aacgccaaac atgccgagga
      901 tccggccccg ctggacgaac gctgccccgg cgactgctgc accaagtaca gccgggccta
      961 tctccaccac ctgcacaaga gcggtgagat cctgggggcc atgctgctga ccgagcacaa
     1021 cctgaccttt taccagcagc ttatggccgc catgcgcgaa gcgataagcg ctggacggtt
     1081 tgcgcagttt gctgtgcaat tccggcggga ttacctccgg acctagtcgc gccgccgggt
     1141 ctccagccgg gtctcgcggt atgccacgaa cattccggta cgccgttcca ggaagccggc
     1201 tccgatcgcg gccgcttgct gccgcgcaag attcagatcc gggtaccatt tgcccacgcg
     1261 gtgcggggtc acgaagcgga acagtttcat gtccggtgca aaggccattc gtgcctccgg
     1321 gtttcgagct ggcgaagacg aatccgccga ctgaaacagg ctggcaccag aaggtgggga
     1381 tagtgctgcg aaacgcggct aaggtgcggc ttgggccgac caggtagatc tgcggagtgg
     1441 gatggtcatg atcctccaac gcgccgtttg gccttgcctc gcaaccctgc taggggcgcg
     1501 gcgcaatgtt tacgatcaac tcagtcaccg tgcggcttgg cggacggacc atcctcgatg
     1561 gcgcttcggc ctcgatcccg cccgggggcc ggatcggcct gatcgggcgc aacggcgccg
     1621 gcaagtcgac gctgatgaag gtgctggtcg gccagctcga tccggacgag ggttcggtcg
     1681 aactgcccaa gcgcacgcgc ctgggctata tcgcgcagga ggccccgagc ggtgagacca
     1741 ccccgttcga ggccgtgctc gccgccgaca ccgagcgcgc cgccttgctg gaagaggcgg
     1801 agcactgcgt cgatccgaac cggctgggcg atctccacga acggctgctg gcgatcgatg
     1861 cctacagcgc cccttcgcgc gccgcgatca tcctcaccgg gctgggcttt gacgaggcaa
     1921 tgcaggccca gccgctcgac agcttttcgg gcgggtggaa gatgcgcgtg gcgctggccg
     1981 cgctgctgtt ctccgcgccg gacgtgctgc tgctcgacga accgtcgaac cacctcgacc
     2041 tggaatcgac gctgtggctg gaaagcttcc tgaagagcta tcccgggacc ctgctggtga
     2101 tcagccacga acgcgacctg ctcaacaacg tggtcgattc gatcctgcac ctgcagggcg
     2161 gcaagctggc gctctatccc ggcaactacg attcgttcga agcccagcgg gccgagcggg
     2221 ccgcgcagct ggccgcggcc aaggcctcgc aggacgcgca gcgcgcgcgg ctgcaggact
     2281 atgtcgcgcg caattcggcc cgcgcctcca ccgccaagca ggcccagtcg cgcgccaaga
     2341 tgctggccaa gatgcagccg attgccgcgc tgatggagga tcccagcctc agcttcgatt
     2401 ttcccagccc caaggaaatg cgcccgccgc tgatcacgct tgatctggcg gccgtggggt
     2461 acaacgacaa gccgatcctg cagcggctca acctgcggat cgatccggat gaccggatcg
     2521 ccctgctggg ccgcaacggc aacggcaaga ccacgctggc gcggctgctg gcggcgcagc
     2581 tggcgccgct ggacggggtg atgcgggcct cgggccagat gacggtcggc tatttcaccc
     2641 agtaccaggt ggaggaactg gccggcgggg atacgccgct ggagcacatg acccgggcga
     2701 tgcccggcgc cgcgccgggg gcggtgcggg cacagctggg ccggttcggc ttttccggga
     2761 accgcgcgac caccaaggtc gggtcgctat ccggcgggga gcgggcgcgg ctggcgctgg
     2821 cgctgatcac ccgcgatgcg cccaaccttc tgatcctcga cgagccgacc aaccacctcg
     2881 acgtcgatgc gcgtgaggcg ctggtccagg cgctcaacga attcgacggc gcggtgatcc
     2941 tgatcagcca cgaccgccac atggtggagc tgaccgccga ccggctggtg ctggtcgata
     3001 acggcacggc cagcgactat gccggcagca tggaggacta catcgacttc gtgctggggc
     3061 ggaaccagcc gaaggccgat gcccggccca agcccgagaa gaaggaccgc aaggccgccg
     3121 ccgaagcgcg cgaacaggcg cgactggccc gcaaggatgt cagcgaggcc gaggccgcca
     3181 tcgcgaagct gcaaaagcag atctccgcga tcgaccaggc gatgttcgcg cccgacagcg
     3241 cggcagcgga cctccgcaag ctgacgatgg gcgaactctc ccgccgccgc ggattggtgg
     3301 aaaaggatct ggccgtggcc gagcagcaat ggctggaggc gagcgagcgg ctggagcagc
     3361 tcgccgcctg aggccggctc agaacccgac gaaccgcgcc gcctcgtcca cgctcttgcg
     3421 ttcggagacg accgcgttgg ccgggaaggt ctcgtccggc cagcccagcg cgaccgcctt
     3481 catgatcacc tgatcgtccg gaatcccggc gtgctcgcgc accaccgggc tctgcatgat
     3541 cccctggctg ttgatcacgc agcccagtcc gcgcgaccag gcggcattga cgagggccgt
     3601 ggtcaccgcg ccgcaatcga aggcggtgtc gtcgctgtcg gccagctcct tgtcataggt
     3661 cacgatcacg caaaccgggg catcgaactg gcggaagccc cgcagcaccc agtcaagccg
     3721 ggcgtccttg tcgtcgcggg cgatgccctg tgcggcaaaa agctgcttgg ccacggcgat
     3781 ctgccgctcg cggtggacac cttcaaaggc atggccggtg cggaattcgc ggctgtgcgg
     3841 caccccggcc aggttgcgtt cggtgttgcc cgcgcggatc cggtccagcg gttcgccagt
     3901 gatcacgtgg aaatgccagg gctgggtgtt catcgagctg ggcgcacgca tcgccagggt
     3961 cagcacctcc tcgatcagtt cgcgcgggac cggcttgtcc agatacccgc ggatcgagcg
     4021 gcggccgagg acgacttcat cgtattccat gctgttcccc ctgatgggcg accttgcggt
     4081 cttggctctc tctttaatca ttcggttaaa ccgcgcaatg gggcaccggg cgcaatccgg
     4141 gccggggccg cccggccaac gctgcggcgg aatccggggc aatttgtgca aggaaatagc
     4201 tttgaagagc cgggttgaac gaattggaat tgttgccatt attgcggcgt tcggatcggc
     4261 tctcgaagtg gagaagaaaa tgccccgact tgcgctcgta atggcggcac ttttgtcaac
     4321 cagcggactt gccgccgcag cgccgcccag cgcggaaagt cttgccctgg cgaacaagtt
     4381 cggtgcgctg gaatcggtcc agcagatgag tctttcgccc gatggcacca agatcgcttt
     4441 cgtcaccccg ctgggcggaa ccagtgcggt gctgaaggtt gcggacctga gcagcggcag
     4501 cgcggttccc aagtcgatcc tgcttcagcg caacgagggg gagacgctgc gcggttgccg
     4561 ttgggccact gacacgcggc tggtttgccg ggtcagcacc atcatcaatt ggggcggcta
     4621 cctgctgccc tttacccggc tgttcgccgt gtcgagcgac gggtcggatt tcaagatgct
     4681 gaccggcggc gtgaagacca gttcgcttgg cgtggttaca ttcggtggca gcgtgatcga
     4741 ctgggacttg ccgggcaagc cgggccgggt gctggtgatg cggcaatatg tgccggatga
     4801 aaacgcgcgg gtgatcagca agctggcgga agaggggatc ggggtcgaag agatcgacgt
     4861 caccacgctg gagcgcacca atatcgagcg cccgaccgac gaaacggtcg agttcatcag
     4921 cgatggccat ggcacggtcc ggatcatggg ccgccagccg cgcaatccgg ccgggtacac
     4981 caagggcacc cgtttctact actaccgcaa gcagggctcc cgcgagtggg aggatctctc
     5041 gaagatcatt tacgatcagg atgcggtggg gcgggggttc gatccctatg cgatcgagta
     5101 ccagcgcaat gcggtgctcg ggttcgatga caacaacggg ttccgggcgc tctaccggat
     5161 cgcgctggac ggttccctga ccaaggagtt ggtcctctcg cgctccgatg tcgatatcga
     5221 ttcggtgatc cagatcggcc gcgacagccg cgttgtcggg gccagctatg cgaccgaacg
     5281 gcgcacggcc gaatatttcg atcccgagct caagaagctc agcgccgcgc tcagccgggc
     5341 cttgccgggg cggcctgtcg tcgatttcgt cgatgccagc atcaacgaac agaagctgct
     5401 ggtattcgcg tccagcgatg tcgatccggg caccttctac cgctacgaca aggcgaccaa
     5461 gcggcttgag ccgatcctgc cgatccgcga gcaactcgcc ggcatggcga tgagcgagat
     5521 gaagccggtg atgatcccgg cggctgacgg cacccaggtg cccggctatc tgaccctgcc
     5581 ggtgggcagc acgggcaaga acctgccggc gatcgtcatg ccgcacggtg gacccggctc
     5641 gcgcgacgaa tgggggtttg actggctggc gcagtacttc gcggcgcgcg gctatgcggt
     5701 ccttcagccc aatttccgcg gatcggccgg ctatggggcg gcctggtacc agaagaacgg
     5761 ctttcaatcc tggcgcaccg cgatcggtga tgtgaacgat gcgggtcgct ggctggtcag
     5821 ccaagggatt gccgcaccgg acaagctggg gatcctgggc tggtcctatg gcggttatgc
     5881 cgcgctccag tcaggggtgc tcgatcccgg gctgttcaag gcggtggtcg cgatcgctcc
     5941 ggtcaccgac ctcgggcggc tgaaggccga tgccgcggac tattccaacg ccccgctggt
     6001 caaccgcttc gtcggcaacg gtccgcacat ccgcgaagga tcgccggccc agaacgccga
     6061 ggccttcacc gtcccggtcc tgatgttcca cggcagcaag gacctcaacg tctcgatcgg
     6121 gcaatcgcgc ctgatggcga cgcggctcag gggcgcgcgc aaggaggtca acctcgttga
     6181 gtttcccggg ctggaccacc agcttggcga cggtgcggcc cgagcccgga tgctgtcgga
     6241 atccgatgcg ttcctgcgcc aggccttccg gatggcgccg tgatcggttg aatcgaaaag
     6301 ggcggccccg caggaccgcc ctttcgtttg gcgttcgccg accggcttag cgcgagtaga
     6361 actcgaccac caggttcggt tccatcttca ccggataggg cacttcatcc agcgtaggca
     6421 cgcggacgaa ggtcgccttg tcggtgccgt cgggcgcgac atagtcgggg atttcacgct
     6481 cgggcaggcc ctgcgcttcg gcgatcaggg ccatttcctt ggccttcttg cccaggctga
     6541 ccacgtcgcc cggacgcacg cggcggctgg cgatgttgca cttcacgccg ttcacataga
     6601 tgtggccgtg cgaaacgatc tggcgggccg aaaagatggt cggcgcgaac ttgcagcggt
     6661 aaaccaccat gtcgaggcgc tgttcgagca ggccgatcag gttctgaccg gtgtcgccct
     6721 tcatgcggct cgcttcctga taggtgcgct tgaactgctt ttcggtgacg tcgccgtagt
     6781 agcccttgag cttctgcttg gcgcggagct ggatcccgaa gtccgagagc ttgcccttgc
     6841 ggcgctggcc gtgctgaccg gggccgtagc tgcggcggtt gaccgagctc ttgggacggc
     6901 cccagatgtt ttcgcccatg cggcgatcga ttttgtactt cgacgactgg cgcttcgaca
     6961 tggcgcaata agtccttcaa cttgcaatgc ggagcgctgt gccccgcttc tggtccggaa
     7021 ccgcaccgcc cgcgcgaagc ggacgcgagc agggcttcac cggagtgccc gctcaattgc
     7081 gaaggcgcgc ccttagcggc agggcgcaca aagatcaagc atcagtcttt ggattcggtg
     7141 acgccggtcg aacgggtggt ggtctgtccg tcatagccag cctcggcctg gtcgaacgcc
     7201 ttccactcct cggccgtgcg gcacacgcgc ttgccggggc ggtggctgcc ggtgcgcggc
     7261 tgggtttcgc ggcacagctt gcggactttg gcgggcttgg ccgcttcttc ggtcttgggg
     7321 gcttcttcgg ccacggccag ggtgggcatg gccagcgcgg cggcaccggc gatcaggaga
     7381 aggcgcatgg aaatccttgg aatgagggtt gaattgggca ggcgactagc agtcccgacc
     7441 ggtcaaggca attcgcgacg gcccttggac agggccgaca ggaccccgcg catggtccgc
     7501 acttccaggt ggttccagcc cggcttggtc agcatgttgc gcaaggtccg ccgggttgcc
     7561 gccgcgcggc tttccgggcg gaaatagccg gtgggctgaa gcaggtcctc gagctgcgtg
     7621 atcatgccct ccagttcctc ctgcggggcc gggggcagaa tttcctcgac cgtgggctgg
     7681 gccaggttgg cgttcttgga ccattcgtag gccagcagga tcaccgcctg ggccagattg
     7741 agcgagccaa actccgggtt gatcggcacg gtgacgatcg cccgggccag ggccacgtcg
     7801 tcggtctcca gccccgagcg ctccggccca aacacatagg ccgaacggcc cggctcggtg
     7861 tggaccgcgc gggcggcctc ttccggggtc agcaccggct tggtcacccc tcgcttgcgc
     7921 acggtggtgg catagacgtg ggcgcaatcg gccacggcct cggccagggt ctcaaagact
     7981 tcggcctttt ccagcaccac gtccgcaccc gccgcggccg ggccggcgct gggattgggc
     8041 cagccgtcgc ggggagcgac cagccgcagt tcggtcagcc cgaaattgag catggcccgg
     8101 gcggccttgc cgatattctc gcccagttgc gggcggacca ggacgataac cgggcgggcc
     8161 ggttcaggac ttgccggcat cgcgcaccgt gctggcaaaa tcctcgaagt ccttggcttc
     8221 ggtgaagtcg cggtagaccg aggcaaagcg gatgtaggcg acggtatcga gcccgcgcag
     8281 gccctccatc accatttcgc cgatcgcctt ggccggaacc tcgctttcgc ccatcgtctc
     8341 gatctggcgc tgcacgcccg agaccagctg gtccatccgc tcctgcgcaa tcccgcgctt
     8401 gcggcaggcg agggcgacgg actggtcgat cttggagcgg tcaaagctct cgcgggtgcc
     8461 atccgatttc accacggtaa tctcgcgcag ctgcacccgt tcgaacgtgg tgaagcgcgc
     8521 gccgcagctg tcgcactggc ggcggcggcg gatcgcggtg ttgtcctcgg tggggcgcga
     8581 atcctttacc tggctgtcat cgtgggcgca gaacggacag cgcacttagg gcctgacgcg
     8641 cttgtagaac atgaaggtcg cgcccaggat cagcccggtc ggccaggcga ccagcggcaa
     8701 gaccgcgccc gcgatggccc ccacggcggc accggtcagc accggcttgg tcgaggggtg
     8761 gcgcaggccg tccttcgcca tgccgctgat ttccgccttg gcgtcgtcca cgaagttgtc
     8821 gctcatggtc tagagtcccg gatagatcgg aaagcgggtg cagagctctt caacccgttc
     8881 gcgcacgcgc tgttccactt ggccgtcgcc ttcatcgccg ttgcgggcca tgccgtcaac
     8941 gacctcggcg atcagctggc cgaccaggcg aaactcctcc tcgccaaagc cgcgggtggt
     9001 gccggcgggg gtgccgagcc ggatgcccga ggtgacgaag ggcgagcgcg tgtcataggg
     9061 cacgccgttc ttgttgcagg tcagccaggc gcgatcgagg cccttttccg cggccttgcc
     9121 ggtcacgtcc ttggccgaga ggtccaccag catcaggtgg ttgtcggtcc cgcccgaaac
     9181 gacccgcagg ccctggtcgg tgacgctggc ggccagcgcc ttggcgttgg cgacgatctg
     9241 ggcggcatag gccttgaatt ccggcttcag cgcatcgcca aaggcgaccg ccttggccgc
     9301 gatcacgtgg accagcggcc cgccctgcag gcccgggaac accgccgagt tgagcttctt
     9361 ggccagatcc tcgtcgttgg tcaggatcac gccggagcgg ggcccgcgca ggctcttgtg
     9421 ggtggtggtg gtgaccacgt gggcgtgggg gaagggcgag gggtgcgcac cgccggccac
     9481 caggccggag aagtgcgaga tatcggccag cagccaggcg cccacttcgt cggcgatttc
     9541 gcggaaccgc ttgaaatccc agtggcgcga ataggcggtg ccgccgcaga tgatcagctt
     9601 gggcttgttc tcccgcgcga tgcgggcaac ctcgtccatg tcgatcagct cgtcctcgcg
     9661 gcgcacgccg taggggatcg gcttgaacca cttgccgctc atgttcacgg ggctgccgtg
     9721 ggtcaggtgc ccgccgcagt tgaggtccag ccccatgaag ctgtcgccgg gctgcagcag
     9781 cgccaggaac acggcctggt tcatctggct gcccgaattg ggctggacgt tggcgaactg
     9841 gcagccgaac agcgccttgg cccgctcgat cgccaggttc tcgaccacgt ccgcgtattc
     9901 gcagccgcca tagtaacgct tccccggata gccttcggcg tacttgttgg tgaagacgct
     9961 gccggtcgct tccagcacgg ccaggctggt gatgttctcg ctcgcgatca gctcgatgtt
    10021 gtggcgctga cgcttcagct cgctctggat cgcggcatgg acctcgggat cggcctcggc
    10081 caggtgcgcg gtgaaaaagc cggccgaacg caccggggcg gcggaaaggg tggtggtcac
    10141 ggggagaggg ctcctcagca gctgggattg gagagctttt cgacccggcc ggcatggcgg
    10201 ccaccgccga aatcggtgga aaggaacgcg tcgagacagg ccttggccat gtccaccccg
    10261 gtcagccggg cgcccatggc gatcacgt
//
LOCUS       DASUDV010000009         9578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1480339,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000009 DASUDV010000000
VERSION     DASUDV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480339"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFV30_00185"
     CDS             <1..495
                     /locus_tag="VFV30_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX4846532.1"
                     /translation="TGETVLIEDGILKGYMQDRLNARLMGVAPTGNGRRESYAHAPMP
                     RMTNTFMKAGNDDPAELLSRIKNGIFAKSFGGGQVDIVSGKFVFSCTEAYKVENGKLG
                     APIKGATLIGDGPSVLTRVKGIGNDMELDEGVGICGKGGQSVPAGVGQPTLLVEGLTV
                     GGTA"
     gene            561..974
                     /locus_tag="VFV30_00190"
     CDS             561..974
                     /locus_tag="VFV30_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846533.1"
                     /translation="MRTFAIALCAASAALLAGPAIEAKPKLTPQQELEKMLEGREPGK
                     PTSCISSFDTREMRVLDKTAIVYGWGNTIWVNRPKNAEHLDDDEIMVIRTNGSQLCSL
                     DIVHTVDRTGGFTTGFISLGEFVPYRKTPKVSAAD"
     gene            1144..2394
                     /locus_tag="VFV30_00195"
     CDS             1144..2394
                     /locus_tag="VFV30_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4846534.1"
                     /translation="MASTATVSRTESPIPPIDVSLPELYAEDRWQEPFRKLRAEAPIQ
                     WVPDSAFGPYWSVTTYKPIVHIEALPKIFSSDWEKGGIAISGSPELLEKYNMREPMFI
                     AMDPPKHTGQRRTVAPSFGPSEVAAMKAECAARTGEVLDSLPIGEEFDWVQRVSIELT
                     TGMLAKLFGFPWEQRHKLTEWSDFGGDVELIKNPETIELRLAKMQEMGMAFAGLWQER
                     MANPGKDLISVMLQSDAMREMEPEEFIGNLILLIVGGNDTTRNSMSAYAYALSKYPEE
                     RAKLEANPALIPNAVQELIRWQTPLSHMRRTVTEDTEIEGVQMKAGDKVVMWYLSANR
                     DESVFEDGEAIRVDRENARRHLSFGYGIHRCVGARVAELQLVTLLEEMAKRRLRAEVL
                     GEPVRVPACFVHGYKSLRVALSRY"
     gene            2394..3047
                     /locus_tag="VFV30_00200"
     CDS             2394..3047
                     /locus_tag="VFV30_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4846535.1"
                     /translation="MSTRAKETRERIIETARVLFNEQGYGAVSTAALAAACGIAEGNL
                     WYHFKTRRALLDALSARFAEAVEARLALVPGADPVASYVRLLEAIMAEFRDFRFLYRD
                     QRSYGEHSDVIAARAPEWIERTYDLVETHLSALVSAGLLDWPAERLRDLAINSTIILR
                     YGLDHYRELGEPTGSGTGAVRRTLQRHLTLFEHRLDPAAAVRLHTAIEAMGAEALAA"
     gene            3113..3607
                     /locus_tag="VFV30_00205"
     CDS             3113..3607
                     /locus_tag="VFV30_00205"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX4846536.1"
                     /translation="MTKPILVLTTGGTIDKNYFDALSEYQIVDSGIPALLQEARVALP
                     HRVVEVMRKDSLELTDADRAAIAAAARAAPEDHIVITHGTDTMTETAKILAREVPGKT
                     ICLTGALSPARFAETDAHFNLGMAFAAAQVAAPGVYIAMSGEVFDGLKVKKDRAAGKF
                     VAVG"
     gene            complement(3625..4839)
                     /locus_tag="VFV30_00210"
     CDS             complement(3625..4839)
                     /locus_tag="VFV30_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX4846537.1"
                     /translation="MIWEPTRAAGLARLQAFLPAAGRAYAASRNVDRGPADRSNVSAL
                     SPWLRRRLITEDEVVAAVLSRHSAAAAEKFVQEVAWRTYWKGWLELRSALLESFNAER
                     IALKDRLERDRDLALRFRRAAEGESGIACFDAWVAELRERGWLHNHARMWFASIWIFT
                     LGLPWQLGADFFYKHLLDADPASNTLSWRWVAGLHTTGKHYLARAENIERNTLGRFAP
                     YGQLNEAAQPLIEADPVPSVTPLAPAGRVGAGPFALLLTEEDLHPESWAPPGEIAAIA
                     MLPSAQVHAPGSPGALFSEGALADAAARAEAHFGRPCARVPAADLPAWLAGKGLTQLV
                     TSQAPTGLIAWQLRDAAAALSAQGAHLIPLRRPWDEALWPLATAGFFKLKTKLPAVIA
                     RLGLDPAAPRLL"
     gene            complement(4856..6415)
                     /gene="guaA"
                     /locus_tag="VFV30_00215"
     CDS             complement(4856..6415)
                     /gene="guaA"
                     /locus_tag="VFV30_00215"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX4846538.1"
                     /translation="MSIQPSDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSSAAAA
                     FQRMKPKGIILSGGPASVLDANGPRIPQEILDSGLPIMAICYGQQALMHQLGGEVLQG
                     DSGEFGRAFIEISDSCVLFDGLWAEGETHQVWMSHGDKVTALAPGFRPVASSPGAPYA
                     VIANDEKRIYAMQFHPEVVHTPDGAKLYRNFVRHVCGLAGDWTMAEFRKTKIEEIRAQ
                     VGKGRVICGLSGGVDSAVAAVLIHEAIGEQLTCVFVDHGLMRLGEAEQVVSLFRGHYN
                     IPLVHKDVSALFLNGLAGVIDPEAKRKFIGKTFIDVFEEEARQVGGADFLAQGTLYPD
                     VIESVSFTGGPSVTIKSHHNVGGLPERMNMSLVEPLRELFKDEVRELGRELGLPEVFV
                     GRHPFPGPGLAIRIPGEVTRERCDILRKADAIYLEEIRNAGLYDAIWQAFAVLLPVKT
                     VGVMGDGRTYDSVCALRAVTSTDGMTADIYPFDAAFLSRVATRIINEVKGINRVVYDY
                     TSKPPGTIEWE"
     gene            6536..7321
                     /locus_tag="VFV30_00220"
     CDS             6536..7321
                     /locus_tag="VFV30_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX4846539.1"
                     /translation="MADAPQTPYPRFPVARDLGRADLTRALAAGARDFAAEPLYGLFF
                     GAIYVLGGLLCWYALVTKGAAAWLIPLAAGFPLIAPFTAVGLYEVSRRREAGLELSWG
                     GVLGALRGRGDDQLLLLGGLIFVGFTFWIILAHGIFAIFAAELGPRGESLALLQTGTG
                     LAMLAVGSAVGALVAFAFYAVTVMSLPMLVDRDVDLLTAIITSLRAVQANRAVLIGWA
                     AFIAALLFAAMVPAFLGLLAALPVLGHATWHLYRRTVSETAVP"
     gene            complement(7318..8865)
                     /locus_tag="VFV30_00225"
     CDS             complement(7318..8865)
                     /locus_tag="VFV30_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX4846540.1"
                     /translation="MAANPAARLEAEFGSFPGLIAAWGEWRGDAPALYDGTTTLSWRE
                     VADRVERIAARLQADGLERGQAVAILGTTTVNYALVYLAAIRAGGCAAPLTTSASPDQ
                     LAGMAADSGAIHLFIDRAKLNELGDFKLPIDRQIVLNEELDSFMAPAGTKAAPFDPAE
                     KDPFNIIYSSGTTGIPKGIVHSHGMRWRQMTFRAALNYGEDSRTICSTPLYSNTTMAV
                     FLPTMYSGGCASLMGKFDVQKWLERASSEHCTHTMLVPVQYRRLMQYEHFDDYDLSAM
                     KMKYCTSAPFPADLKAEVLKRMPGGLIEIYGMTEGGVACIFPVHEHPDKLHTVGKPAP
                     GHFLKVLDEEGNEVPPGTPGELVGISPTMMLGYKNQPEKTREGYWINPEDGSVWQRMG
                     DVGRVDEDGFVELVGRTKDVIISGGFNIFPIDLEAELAKDDRVVEAAVVGVPSEKWGE
                     TPVGFVVLKPGVGEDALEDIRSVANSRLGKTQRVAELYAIGEMPRSHIGKLLKTDLRE
                     LVPAKPR"
     gene            complement(9433..>9578)
                     /locus_tag="VFV30_00230"
     CDS             complement(9433..>9578)
                     /locus_tag="VFV30_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX4846541.1"
                     /translation="DQYRARWNLPADYPMVAPNYAEKRRDLAKKIGLGRKPGQKRGRK
                     PKA"
BASE COUNT         1540 a         3188 c         3249 g         1601 t
ORIGIN      
        1 accggcgaga ccgtgctgat cgaggacggg atcctgaaag gctatatgca ggaccggctc
       61 aacgcccggc tgatgggcgt cgcccccacc ggcaacggcc gccgcgaaag ctatgcccac
      121 gcgccgatgc cgcggatgac caacaccttc atgaaggccg ggaacgacga tccggccgag
      181 ctgctgagcc ggatcaagaa cgggatcttc gccaagagct ttggcggcgg gcaggtcgat
      241 attgtgtcgg gcaagttcgt gttctcctgc accgaggcct acaaggtcga gaacggcaag
      301 ctcggcgcgc cgatcaaggg cgcgaccctg atcggcgacg ggcccagcgt gctgacccgg
      361 gtcaagggca tcggcaacga catggaactg gacgaaggcg tcggaatctg cggcaagggc
      421 gggcaatcgg ttcccgccgg ggtcggccag ccgaccctgc tggtcgaagg cctgaccgtg
      481 ggcggaaccg cctgaccgct taagccttgg ttcaggatgg ctgggccagc ttgggccgcg
      541 agggaaagag gagcaagacc atgcgcacgt ttgccattgc cctgtgtgcc gcttcggccg
      601 cgctgctggc cggtccggcg atcgaggcca agcccaagct gaccccgcag caggaactgg
      661 aaaagatgct ggaagggcgt gagcccggca agccgaccag ctgcatctcc tcgttcgata
      721 cccgtgagat gcgggtgctc gacaagaccg cgatcgtcta tggctggggc aacaccatct
      781 gggtcaaccg gcccaagaat gccgagcatc tggacgacga cgagatcatg gtgatccgca
      841 ccaacggcag ccagctgtgc agcctcgaca ttgtccacac ggtcgatcgc accggcggct
      901 tcaccaccgg cttcatcagc ctgggtgaat tcgtgcccta ccgcaagacg cccaaggtct
      961 ccgcggccga ctgatcccgc ctggaccccc ccctgcacca acccttgcag cgccacgccg
     1021 tccggagcga tccgggcggc gtcggtgcgt ctggggccgc gcatccgggc gatggggctt
     1081 gcgcttcggc caaattaggg tattcaccct aaaaagccga atccgagctt gagggagaat
     1141 gccatggcca gcaccgccac cgtttcgcgc accgaatcgc cgatcccgcc gatcgatgtg
     1201 tcgctgcccg aactctatgc cgaagaccgc tggcaggagc cgttccgcaa gctgcgggcc
     1261 gaggcgccga tccagtgggt gccggacagc gcctttggac cctactggtc ggtcaccact
     1321 tacaagccga tcgtccatat cgaggcgctg cccaagatct tctcgtccga ctgggaaaag
     1381 ggcggcatcg ccatttccgg cagcccggaa ctgctggaaa agtacaacat gcgcgagccg
     1441 atgttcatcg cgatggaccc gcccaagcac accggccagc gccgcaccgt ggcccccagc
     1501 tttggcccca gcgaagtggc cgcgatgaag gccgaatgcg cggcgcggac cggcgaagtg
     1561 ctcgattcgc tgccgatcgg ggaagagttc gactgggttc agcgggtctc gatcgagctg
     1621 accacgggga tgctggccaa gctgttcggc tttccctggg aacagcgcca caagctgacc
     1681 gaatggtccg acttcggcgg cgatgtggag ctgatcaaga accctgaaac gatcgaactg
     1741 cggctcgcca agatgcagga aatgggcatg gcgtttgccg ggctgtggca ggaacggatg
     1801 gccaatcccg gcaaggacct gatctcggtc atgctccagt ccgacgcgat gcgcgaaatg
     1861 gagcccgagg agttcatcgg caacctgatc ctgctgatcg tcggcgggaa cgacaccacc
     1921 cgcaattcga tgtcggccta tgcctatgcg ctcagcaagt accccgaaga acgggccaag
     1981 ctggaagcca atccggcgct gatccccaac gcggtgcagg aactgatccg ctggcagacc
     2041 ccgctttcgc acatgcgccg caccgtgacc gaggataccg agatcgaagg cgtgcagatg
     2101 aaggccggcg acaaggtggt gatgtggtac ctttccgcca accgcgacga aagcgtgttc
     2161 gaggacggcg aggcgatccg ggtcgaccgc gagaacgccc gtcggcacct gtccttcggc
     2221 tatggcatcc accgctgcgt cggggcccgg gtggccgaac tgcagctggt caccctgctg
     2281 gaggaaatgg ccaagcgccg cctgcgcgcc gaagtgctgg gcgaaccggt ccgcgtgccg
     2341 gcctgcttcg tgcacggtta caagtcgctg cgggttgcgc tatcgcgcta ctgatgtcga
     2401 cacgcgccaa ggaaacccgg gagcggatta tcgagaccgc gcgggtcctg ttcaacgaac
     2461 agggctatgg cgcggtgtcg accgcggcgc tggcggcggc ctgcgggatc gccgaaggca
     2521 acctgtggta ccatttcaag acccgccgtg cgctgctcga tgcgctcagc gcgcgctttg
     2581 ccgaggcggt ggaagcgcgc ctggcgctgg ttcccggggc cgatccggtg gccagctatg
     2641 tccgcctgct cgaagccata atggcggaat tccgcgactt tcgcttcctc taccgcgacc
     2701 agcgcagcta tggtgagcac agcgatgtga tcgccgcgcg cgcgccggaa tggatcgagc
     2761 ggacctatga cctggtcgag actcacctct ccgcgctggt ctctgccggc ttgctcgact
     2821 ggccggccga gcggctgcgc gacctggcga tcaacagcac gatcatcctg cgctacggcc
     2881 tcgatcacta ccgcgaactg ggcgagccga ccggatcggg cactggcgcg gtgcggcgca
     2941 ccttgcagcg gcatctgacc ctgttcgaac accgcctcga tcccgccgcc gcggtgcgtc
     3001 tccacaccgc gatcgaggcg atgggggccg aggcgctggc ggcgtaagag gcgtccttca
     3061 gcagggccgg tgttgcttcc cctttagggg aacaggttct atggcgcggg gcatgaccaa
     3121 gcccattctc gtcctcacca ccggcggcac gatcgacaag aactacttcg acgcgctgag
     3181 cgagtaccag atcgtcgatt ccggcattcc ggcgctgctt caggaggcgc gggtggcgct
     3241 gccgcaccgc gtggtcgaag tgatgcgcaa ggatagcctg gagctgaccg acgccgaccg
     3301 cgccgcgatt gccgcggccg cgcgcgcggc gcccgaggac cacatcgtga tcacccacgg
     3361 caccgacacc atgaccgaaa cggccaagat cctggcccgc gaagtgcccg gcaagacgat
     3421 ctgcctgacc ggcgcgctca gccctgctcg cttcgccgag accgacgcgc acttcaacct
     3481 cggcatggcc ttcgccgccg cgcaggtcgc cgcgcccggc gtctacatcg cgatgagcgg
     3541 cgaggtgttc gacggcctga aagtgaagaa ggaccgcgcg gcggggaagt tcgtggcggt
     3601 tggctagaac agggtcccgc gttcctacag cagccgcggc gctgccggat ccagccccag
     3661 ccgggcgatc accgccggaa gtttcgtctt cagcttgaag aaaccggccg tggcgagcgg
     3721 ccagagcgct tcgtcccagg ggcggcgaag cgggatcagg tgggcaccct gcgcggacag
     3781 ggccgccgcg gcatcgcgca gctgccaggc gatcaggccg gtcggcgcct ggctggtgac
     3841 cagctgggtc aggcccttgc cggcaagcca ggcgggcagg tctgccgctg gcactctggc
     3901 gcaaggccgg ccgaaatgcg cctctgcccg cgcggccgca tcggccaggg caccctccga
     3961 aaacagcgcg ccggggctgc cgggggcgtg gacctgggcg ctgggcagca tcgcaattgc
     4021 cgcgatctcg cccggcgggg cccagctttc ggggtgaagg tcttcctcgg tcagcagcag
     4081 ggcgaagggc ccagcgccca cccgcccggc cggagcgagc ggggttacgg acggcaccgg
     4141 atccgcctcg atcagcggct gggccgcttc gttgagctgg ccataggggg cgaaccggcc
     4201 cagcgtgttg cgctcgatat tctcggcccg ggccagatag tgtttccccg tagtgtgcag
     4261 ccccgcgacc cagcgccacg acagcgtgtt ggacgccgga tcggcatcga gcaggtgctt
     4321 gtaaaagaaa tccgcgccca gctgccacgg caggcccagc gtaaagatcc agatgctggc
     4381 gaaccacatc cgcgcgtggt tgtgcagcca gccgcgttcg cgcagctcgg ccacccaggc
     4441 atcgaagcag gcgatcccgc tttcgccctc ggccgcgcgg cgaaagcgca gggccagatc
     4501 acggtcccgc tccagccggt ccttcagcgc gatccgttcg gcgttgaagc tttccagcag
     4561 ggccgagcgc agctccagcc agcccttcca ataggtccgc caggccactt cctgcacgaa
     4621 cttctccgcc gccgcggcgc tgtggcggga caggacggcg gccaccacct cgtcctcggt
     4681 gatcaggcgg cggcgcagcc atggcgacag ggcggagacg ttcgagcggt cggccggccc
     4741 gcgatcgaca ttgcggctgg cggcataggc gcgcccggca gcgggcagaa aggcctgcag
     4801 ccgcgccagc ccggccgcgc gggtcggctc ccagatcatg ccgcgcgctg acggatcatt
     4861 cccactcgat cgtgcccggc ggcttcgacg tgtagtcgta gaccacgcgg ttgatgccct
     4921 tgacctcgtt gatgatccgg gtcgcaacgc ggctgaggaa ggcggcgtcg aagggataga
     4981 tgtccgcggt catgccgtcg gtgctggtca cggcgcgcag cgcgcagacg ctgtcatagg
     5041 tgcggccatc gcccatcacg ccgaccgtct tgaccggcag cagcacggcg aaggcctgcc
     5101 agatcgcgtc gtaaagcccg gcgttgcgga tttcttcgag gtaaatcgcg tcggctttcc
     5161 gcaggatatc gcagcgctcg cgcgtcactt cgccggggat gcggatcgcc aggcccggtc
     5221 cggggaaggg gtggcggcca acgaagacct cgggcaggcc gagttcgcgg cccagttcgc
     5281 ggacctcgtc cttgaacagt tcgcgcaagg gttcgacgag gctcatgttc atgcgttcgg
     5341 gcaggccgcc gacgttgtgg tggctcttga tcgtcacgct cggcccgccg gtgaagctga
     5401 cgctctcgat cacgtccgga tagagcgtgc cctgggcgag gaagtccgcc ccgccgactt
     5461 ggcgggcctc ttcctcgaac acgtcgatga aggtcttgcc gatgaacttg cgcttggcct
     5521 cggggtcgat caccccggcc aggccgttca ggaacagcgc gctgacgtcc ttgtggacca
     5581 gcgggatgtt gtagtgcccg cggaacagcg aaacgacctg ctcggcctcg cccagccgca
     5641 tcaggccgtg gtcgacgaag acgcaggtca gctgctcgcc gatcgcctcg tggatcagca
     5701 cggcggccac cgcgctgtcg actccgccgg aaagcccgca gatcacccgg cccttgccga
     5761 cctgggcgcg gatttcctcg atcttggtct tgcggaattc ggccatggtc cagtccccgg
     5821 ccaggccgca gacgtggcgg acgaagttgc ggtacagctt cgcgccgtcc ggggtgtgga
     5881 ccacttcggg gtggaactgc atcgcgtaga tgcgcttctc gtcgttggcg atcaccgcat
     5941 agggcgcgcc gggcgagctg gcgaccgggc ggaagccggg ggccagcgcg gtcaccttgt
     6001 cgccgtggct catccacacc tggtgggtct cgccctcggc ccacagcccg tcgaacagca
     6061 cgcagctgtc cgaaatctcg ataaaggcgc ggccaaactc gccgctgtca ccctgcagca
     6121 cttcgccgcc cagctggtgc atcagcgcct gctggccata gcagatcgcc atgatcggca
     6181 ggccgctgtc gaggatctcc tgcgggatcc gcgggccgtt cgcatcgagc accgaggccg
     6241 gcccgcccga gaggatgatc cccttgggct tcatccgctg gaacgcggcg gcggcgctgc
     6301 tgaacggggc gatctcggaa tagacccccg cttcgcgcac gcggcgggca atcagctggg
     6361 taacctggct gccgaaatcg acgatgagga tggaatcgga aggctggatg ctcatggctg
     6421 gccgataagg gccgtgcggg ccggtggcaa gggcaggcgc tgcgctgcca acacttgacg
     6481 cgggtcaagg accgcggcgc catggctggc aaggtgccgg gcgggagaga gaccgatggc
     6541 cgatgcaccg cagaccccct atccgcgctt tccggtggcc cgtgacctcg gccgggccga
     6601 tctgacccgg gcgctggccg cgggcgcgcg cgattttgcc gccgagccgc tctatggcct
     6661 gttcttcggc gcgatctatg tgctgggcgg tttgctgtgc tggtacgcgc tggtcaccaa
     6721 gggggcggcc gcctggctga tcccgctcgc cgccggattt ccgctgatcg cgccgttcac
     6781 cgcggtcggg ctgtacgagg tcagccgccg gcgcgaggcc gggctggagc tcagctgggg
     6841 cggcgtgctc ggtgcgctgc gcgggcgggg ggacgatcag ctgctgttgc tcggcgggct
     6901 gatcttcgtc ggcttcacct tctggatcat cctcgcccac gggatcttcg cgatcttcgc
     6961 ggccgagctg ggcccgcgcg gggaatcgct ggcgctgctg cagaccggca cggggcttgc
     7021 catgctggcg gtcggcagcg cggtcggcgc gctggtcgct ttcgcgttct acgcggtgac
     7081 ggtgatgagc ctgccgatgc tggtcgatcg cgatgtcgat ctgctgaccg cgatcatcac
     7141 cagcctgcgg gcggtccagg ccaaccgggc ggtcctgatc ggctgggcgg cgttcattgc
     7201 cgcgctgctg ttcgccgcga tggtgccggc cttcctgggg ctgctggcgg cgctgccggt
     7261 gctgggccac gccacctggc acctctaccg ccgcacggtc agcgaaaccg ccgtgcccta
     7321 gcgcggcttg gcgggcacca gttcgcgcaa gtccgtcttg agcagcttgc cgatgtggct
     7381 gcgcggcatt tccccgatcg catagagctc ggccacgcgc tgggtcttgc cgaggcggct
     7441 gttggcgacg ctgcggatgt cttcgagcgc gtcttcgccc acaccgggtt tcaggacgac
     7501 aaagccaacc ggggtttcgc cccacttctc gctgggcacg ccgaccacgg cggcttcaac
     7561 cacgcggtcg tccttggcca gttcggcttc caggtcgatc gggaagatgt tgaacccgcc
     7621 cgagatgatc acgtccttgg tccgcccgac cagttcgaca aagccgtctt cgtcgacccg
     7681 gccgacatcg cccatccgct gccagaccga tccgtcctcg gggttgatcc aatagccctc
     7741 gcgggtcttt tccggctggt tcttgtagcc cagcatcatc gtgggcgaga tgccgaccag
     7801 ttcgcccggc gtgcccggcg gcacttcgtt gccctcctcg tccagcactt tcaggaagtg
     7861 cccgggggcg ggtttgccca cggtgtggag cttgtccggg tgttcgtgga ccgggaagat
     7921 gcaggccacg ccgccttcgg tcatgccgta gatctcgatc aggccgccgg gcatccgctt
     7981 cagcacttcg gccttgagat cggcagggaa gggggcgctg gtgcagtact tcatcttcat
     8041 cgccgacagg tcgtagtcgt cgaagtgctc gtactgcatc agccgtcggt actggaccgg
     8101 gaccagcatg gtgtgggtgc agtgttcgct gctggcgcgc tccagccatt tctggacgtc
     8161 gaacttgccc atcaggctgg cgcagccgcc ggaatacatc gtcggcagga acaccgccat
     8221 ggtggtgttg gaatagagcg gggtcgaaca gatcgtccgg ctgtcctcgc cgtaattgag
     8281 cgcggcgcgg aaggtcatct gccgccagcg catcccgtgg ctgtggacga tgcccttggg
     8341 aatgccggtg gtgccgctgg aatagatgat gttgaagggg tccttctcgg ccggatcgaa
     8401 cggcgcggcc ttggtcccgg ccggggccat gaagctgtcc agctcctcgt tcagcacgat
     8461 ctgccggtcg atcggcagtt tgaaatcgcc cagctcgttg agcttcgccc ggtcgatgaa
     8521 caggtggatc gcgcccgaat ccgcggccat gccggccagc tgatcggggc tggcgctggt
     8581 ggtcagcggc gcggcgcagc ccccggcgcg gatcgcggcg agatagacca gcgcatagtt
     8641 caccgtggtc gtccccagga tcgccacagc ctgcccgcgc tccagcccgt cggcctgcag
     8701 ccgcgcggca atccgctcga cccggtcggc cacttcgcgc caggacaggg tggtggtgcc
     8761 gtcatagagc gccggggcgt cgccgcgcca ctcgccccag gccgcgatca gcccgggaaa
     8821 ggaaccgaat tcggcttcaa gccgggcggc ggggttggcg gccatgggtg tcctctcgtc
     8881 catgtggaat catttccggt tgctttgcgc aacccaccgg gcgctgtcca gcccgcaacc
     8941 gatccttacc gccgtcctaa agttccggtt gcaaataatg caacgaccct ttcggccttt
     9001 gcactggtaa tctctaccag aatgcttatg ttgcaaatgc gaactgagtt agccaatgca
     9061 aacaggagaa accccatgcg cagcatcgct tccgtcgtgc tgccgatcat cgccgcggct
     9121 tcgttcagcg gtctgatgtt ttccgccacg cttgcctaag cgcagcggat cggcacacct
     9181 cccaacagca aagggccgac ccgcctaggc aggtcggccc tttgttttga gtcttggtcg
     9241 ggtgcgctga tcgcctccgc gatcagcttg gcctggccgg cccgctcccc cggcccaacc
     9301 acccgttaca tggtacccgt ggggtggttg gggcgccccg acaaaacgtc ccccggacgt
     9361 tttgcgaatt cgtgacacgg gagcgggccg gtgatgcaag ggcaggccgg agggcctgcc
     9421 cgcaccagac aattaagcct tcggcttgcg gccgcgcttc tggccgggct tgcggcccag
     9481 gccgatcttc ttggccaggt cgcggcgctt ttcggcgtag ttgggggcaa ccatcggata
     9541 gtcggcgggc aggttccagc gcgcgcggta ctgatccg
//
LOCUS       DASUDV010000010         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1215296,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000010 DASUDV010000000
VERSION     DASUDV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215296"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFV30_00235"
     CDS             complement(<1..830)
                     /locus_tag="VFV30_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4846542.1"
                     /translation="MGYEPQADFGTPESRSETLVTLRIDGRDVTVPQGTSVMRAAAEC
                     GGQIPKLCATDNVKAFGSCRMCLVEVDGMRGTPASCTTPVAEGMVVHTQTPRLEKLRR
                     GVMELYISDHPLDCLTCSANNDCELQDTAAQVGLRDVRYGYSGANHLALPTDTSNPYF
                     DFDPSKCIACSRCVRACDEVQGTLALTVEGRGFGSKISAGQAGDDFLSSECVSCGACV
                     QACPTATLQEKAVKEIGKPERAVVTTCAYCGVGCTFRAEMRGEQLVRMVPWKDGKANR
                     G"
     gene            complement(830..2344)
                     /locus_tag="VFV30_00240"
     CDS             complement(830..2344)
                     /locus_tag="VFV30_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX4846543.1"
                     /translation="MTVVRISDDAQALACGADSVAAAFAAAGCTVERVSSWGMHWLEP
                     LVEIDGNGFGPAVPGDVAAILDGSSAKAIGPIADHPFIARQQRLTFARAGKTRPLDLD
                     DYAASGGWSGLRRARAMDPADVVAEVLESGLRGRGGAGFPAGIKWKTVLEAAGPTKYI
                     VCNADEGDSATFADRMLMEGDPFQLLEGMAIAGHAVGAGEGFIYVRSEYPHAIAKLNA
                     AIARAAGELAPFRIEVRVGAGAYVCGEETSLLNSLEGKRGEVRAKPPLPALEGLFGCP
                     TVVNNVLTLAAVPHILAEGGAAVYARLGIDRSKGTMPIQLAGNIRHGGLFETAFGITL
                     RELVEDIGGGTASGRPVKAAQVGGPLGAYIPPSKFDLPFDYEAYAGADALIGHGGISV
                     FDDTADMGAMARFAFEFCAAESCGKCTPCRLGSTRGVELIDRIRAATPAEADPVAALP
                     QMHNARKAARSREDELQLLEDLCETMQFGSLCALGGFTPYPVRSALKHWPEDFN"
     gene            complement(2341..2763)
                     /locus_tag="VFV30_00245"
     CDS             complement(2341..2763)
                     /locus_tag="VFV30_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX4846544.1"
                     /translation="MSGETLDSIIAEHRGRKGALLPMLHAIQHAFGCIDAKAEAAVAQ
                     ALNLSRAEVHGVVSFYHDFTPAPDPRPVVELCRAEACKARGVEALVPAAEAAAGERVN
                     LKTVYCLGLCATGPNARTGDTLHSRLDEAALVNLVRSA"
     gene            complement(3104..3991)
                     /locus_tag="VFV30_00250"
     CDS             complement(3104..3991)
                     /locus_tag="VFV30_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4846545.1"
                     /translation="MQLQYLRYFVALARHRHFAEAANACGVSQPTLSAGLAALEQELG
                     KRLIDRDRRFIGLTEHGEAILPWAEQALGAVRGLSQAAAATTAGPAGEFRLAAIPSAL
                     PLAGRLGEALLSAHPGLTMAVQTGTWREIERDLQANECDAGLTYLDHDPPANALSVPL
                     HAEQYLFAVRAGGAFDGQPSVTWQDVADQPLCLLHQGMQYRRILDREFAGRGLSVNPR
                     AIADSHVTLLALVQTGAFSTIVPDVYARLMEGLDWCRFLPIEPEAGTRRIGLVVVNRD
                     PLGTMARLGLIAAQQLQAP"
BASE COUNT          670 a         1422 c         1372 g          650 t
ORIGIN      
        1 ccgcggttgg ccttgccgtc cttccacggc accatccgga ccagctgttc cccgcgcatt
       61 tccgcgcgga aggtgcagcc caccccgcaa taggcgcagg tggtgacgac cgcgcgctcc
      121 ggcttgccga tctccttgac cgccttttcc tgcaaggtcg cggtcgggca ggcctgcacg
      181 caggcgccgc agctgacgca ttcgctggag aggaaatcgt cgccggcctg gcccgcgctg
      241 atcttgctgc cgaacccgcg tccttcgaca gtcagggcca gagtgccctg cacttcgtcg
      301 caggcccgca cgcagcgcga gcaggcgatg cacttcgacg gatcgaaatc gaaatagggg
      361 ttcgaggtgt cggtcggcag ggccagatgg ttggccccgg agtagccgta acggacatcg
      421 cgcagaccga cctgagcggc ggtatcctgc agttcgcaat cgttgttggc cgaacaggtc
      481 aaacagtcca gcgggtgatc ggagatgtag agctccatca ccccgcggcg cagcttttcc
      541 aggcgcgggg tctgggtgtg gaccaccatg ccttcggcca ccggcgtggt gcagctggcg
      601 ggcgtgccgc gcatcccgtc aacctcgacc aggcacatcc ggcacgaacc gaacgccttg
      661 acgttgtcgg tcgcgcaaag cttggggatc tgaccgccgc attcggccgc tgcgcgcatc
      721 acgctggtgc cttgcggcac ggtcacatcg cgcccgtcga tccgcagggt gaccagcgtt
      781 tcggagcggc tttccggggt gccgaaatcg gcctggggtt cgtagcccat cagttgaaat
      841 cctccggcca gtgtttgagc gccgaacgca ccggataggg cgtgaagccc cccagcgcgc
      901 aaagcgaacc gaattgcatg gtctcgcaca ggtcttcgag cagctggagc tcgtcctcgc
      961 ggctgcgcgc ggccttgcgg gcgttgtgca tctgcggcag cgctgccacc ggatcggcct
     1021 cggccggcgt ggcggcccgg atccggtcga tcagctccac cccgcgggtc gagccgaggc
     1081 ggcagggggt gcacttgccg cagctttccg cggcgcagaa ttcaaaggca aagcgcgcca
     1141 tggcgcccat gtcggccgtg tcgtcaaaca ccgaaatgcc gccgtgcccg atcagcgcat
     1201 cggcgccggc ataggcttcg taatcgaacg gcaggtcgaa cttgctgggc ggaatgtagg
     1261 cccccagcgg cccgccgacc tgcgccgcct tgaccgggcg gcccgaagcc gtcccgccac
     1321 caatatcctc gaccagttcg cgcagggtga tgccaaatgc ggtctcgaac agcccgccgt
     1381 ggcggatgtt gccggccagc tggatcggca tggtgccctt ggaccggtcg atcccgagcc
     1441 gcgcgtaaac cgccgccccg ccctcggcca ggatgtgcgg cacggcggcc agggtcagca
     1501 cgttgttgac cacggtgggg cagccgaaca gcccttccag cgccggcagc ggcggcttgg
     1561 cgcgcacttc gccgcgcttg ccttccagcg agttgagcag cgaggtttcc tcaccgcaga
     1621 cataggcccc ggcgccaacg cggacctcga tccggaacgg cgcgagctcg cccgcggccc
     1681 gggcgatcgc cgcgttcagc ttggcaatgg cgtgggggta ttcgctgcgg acatagatga
     1741 agccctcgcc cgcgccaaca gcgtggccgg caatcgccat gccttcgagc agctggaacg
     1801 gatcgccttc catcagcatc cggtcggcaa aggtcgcgct gtcgccctcg tcggcgttgc
     1861 agacgatata cttggtcggc ccggccgctt cgagcacggt cttccacttg atgcccgccg
     1921 ggaagcccgc cccgccccgg ccgcgcaggc cggattcgag cacttcggcc accacatcgg
     1981 ccgggtccat ggcgcgggcg cggcgcaggc cgctccaccc gccgctggcc gcgtaatcgt
     2041 ccagatcgag cgggcgggtc ttgcccgcac gggcaaaggt cagccgctgc tggcgggcaa
     2101 tgaagggatg gtcggcaatc gggccgatcg ccttggcgga cgagccgtcc agaatggccg
     2161 cgacatcgcc gggcaccgcc gggccaaagc cgttgccgtc aatctccacc agcggttcca
     2221 gccagtgcat cccccacgag gagacgcgct caaccgtgca accggctgcg gcaaaggcgg
     2281 ccgccacgct gtccgcgccg caggccaggg cctgagcgtc gtcgctgatc cgcaccaccg
     2341 tcatgccgat ctcaccaggt tgaccagcgc cgcttcatca agccgcgaat ggagagtgtc
     2401 gcccgtgcgg gcgttgggcc cggtggcgca gaggcccagg caatagacgg tcttgaggtt
     2461 gacccgctcg cccgccgcgg cctctgccgc cgggaccagc gcttcgaccc ctcgggcctt
     2521 gcaggcctcg gcgcgacaca gctccaccac cgggcgcgga tcgggcgcgg gggtgaaatc
     2581 gtgatagaag ctgaccaccc cgtgcacttc cgcgcgcgag aggttcaggg cctgggcaac
     2641 ggcggcctcg gccttggcgt cgatgcagcc gaacgcgtgt tggatcgcgt gcagcatggg
     2701 cagcaaggca cccttgcggc cgcggtgctc cgcgataatg ctatccaaag tttccccact
     2761 catcgccttc ccctgaacgg gcgcaagggc ctggtcaaat cgaacgatgc gaagattaat
     2821 agatctgatc tattagggtc cggacccatt accagcaggt ccgaggcggc aaaatggcga
     2881 acatatcggg ccgaaaggca cgctgcaggg gctggttgcc cctgcaaggg cgttttggtc
     2941 cgagatggaa gccatttggc cgcccctgcg gggtttgata aatttgcctc attgctgcgt
     3001 tgggaagtct tgaaatatcg acatattcct tcgacttccc gccttgcact gaggcaaatt
     3061 ccttcaaacc tcggacctgc tggtaatggg cccggaccct taatcagggc gcttgcagct
     3121 gctgcgcggc gatcaggccc agccgggcca tggtccccag cgggtcgcga ttgaccacca
     3181 ccaggccgat ccggcgggtg ccggcctcgg gctcgatcgg caggaaccgg caccagtcca
     3241 gcccttccat cagccgggca tagacatcgg gaacgatggt cgaaaatgcg ccggtctgga
     3301 ccagcgccag cagggtcacg tggctgtcgg caatcgcccg cgggttgacc gaaaggcccc
     3361 gcccggcgaa ctcgcggtcc aggatccggc ggtactgcat cccctggtgc agcaggcaca
     3421 gcggctggtc ggctacgtcc tgccaggtca cgctgggctg gccgtcgaag gcgcccccgg
     3481 cccgcaccgc aaacaggtat tgttcggcgt ggagcggcac ggacagcgcg ttggcgggcg
     3541 gatcgtggtc caagtaggtc agcccggcat cgcattcgtt ggcctggaga tcgcgctcga
     3601 tctcgcgcca ggtgccggtc tgcacggcca tggtcagacc cggatgggcc gagagcagcg
     3661 cctcgcccag ccgccccgcc agcggcaggg ccgaagggat cgcggccagc cggaactcgc
     3721 ccgccgggcc cgcggtggtg gccgccgcgg cctggctcag cccgcgcacc gcgcccagcg
     3781 cctgctcggc ccagggcagg atcgcctcgc catgctcggt caggccaatg aagcgccggt
     3841 cgcggtcgat cagccgcttg cccagctcct gctccagcgc ggccagcccg gccgagaggg
     3901 tcggctggct caccccgcag gcgttggccg cctcggcaaa atggcggtga cgggcgagcg
     3961 cgacgaaata gcgcaggtac tggagctgca tgaaacctcc ggcctaagag gttccgtcta
     4021 tcacatgaca ggttaagagc ctatcgccga aacccggctc agcattccac cacgttgacg
     4081 gcaagcccgc ccagcgaggt ttccttgtac ttgt
//
LOCUS       DASUDV010000011         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1391996,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000011 DASUDV010000000
VERSION     DASUDV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391996"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFV30_00255"
     CDS             complement(<1..1023)
                     /locus_tag="VFV30_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4846546.1"
                     /translation="MAEPRTRGSWKRRTSLTVRILAVNIIALGLMAGSLFYLDSYRKQ
                     VLSERFRLAAAEAEIAAEGLRAADPARRPGLLAEIGKDQELRLRHYGPDGRLLADSFA
                     QGPSFVLADPQGQDLLIKAARVIDRAMNWVLGSDPIPAYRDPPRDVAAAWPELGEARA
                     GRTSVIRLRAAPDETPMINAAVPVGNDGSTLLATRNAPDITQAVRDARGTLAIVLAVT
                     LLISIQLSLFLARTIVQPLRALVRAAVRVRLGRDRAVEVPRLPERGDEIGLLARALSD
                     MTGALRKRIDAVETFAADVAHEIKNPLASLRSALESLEKVKDEQLRAELTAIATHDVR
                     RIDRLVT"
     gene            complement(1016..1762)
                     /locus_tag="VFV30_00260"
     CDS             complement(1016..1762)
                     /locus_tag="VFV30_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4846547.1"
                     /translation="MAENQPSPPPSAQQVIALVDDDRNILTTVSIGLQAEGFATRVYS
                     DGATALKALLDNPPDLVVCDIKMPRMDGLELLQKLRAKSALPLIFLTSKDEEPDEALG
                     LAMGADDYIAKPFSQRLLVARIRAILRRAELVRGEAEAQPDPDAGSETFVRGRLEMDP
                     TRHRVSWAGQPVSLTVTEFLILEALALRPGVIKSRNQLMDAAYSDDVFVDDRTIDSHI
                     KRLRRKFRSVDREFAAIETLYGAGYSFADG"
BASE COUNT          297 a          682 c          712 g          319 t
ORIGIN      
        1 ggtgaccagc cggtcgatcc gccgcacgtc gtgcgtggcg atcgcggtca gttcggcgcg
       61 gagctgctcg tccttgacct tttccagcga ttcgagcgcg ctgcgcagcg aggcgagcgg
      121 gttcttgatc tcgtgcgcga catcggcggc gaaggtttcc accgcgtcga tccgcttgcg
      181 cagggcgccg gtcatgtccg agagggcgcg ggcgagcagg ccgatctcgt cgccgcgttc
      241 gggcaggcgc ggaacctcca ccgcgcggtc gcgccccagg cggacccgca ccgccgcccg
      301 caccagcgcc cgcagcggct ggacgatggt ccgcgccagg aacagcgaga gctggatcga
      361 gatcagcagg gtcaccgcca ggacgatcgc cagggtgccg cgcgcgtcgc gcaccgcctg
      421 ggtgatatcg ggcgcgttgc gggtggcaag cagggtgctg ccgtcgttgc ccaccggcac
      481 cgcggcattg atcatcgggg tttcgtctgg cgcggcgcgc aggcggatca cgctggtgcg
      541 gcccgcgcgc gcctcgccca gttccggcca ggccgccgcc acatcgcgcg gcggatcgcg
      601 gtaggccggg atcggatcgg agccgagcac ccagttcatc gcccggtcga tcacccgcgc
      661 ggccttgatc agcaggtcct gcccctgcgg atcggccagg acgaagctgg gcccctgggc
      721 aaaactgtct gccagcaggc ggccatcggg cccgtagtgg cgcaggcgca gctcctggtc
      781 cttgccgatc tctgccagca ggccggggcg ccgcgcggga tcggcggcgc gcaggccttc
      841 ggcggcgatc tccgcttcgg ccgcggccag gcgaaagcgt tcggacaaga cctgcttgcg
      901 gtaggaatcg aggtagaaca ggctgcccgc catcagcccc agggcgatga tgttcaccgc
      961 caggatccgc acggtcaggc tggtccggcg tttccagctg ccccgggtgc gcggctcagc
     1021 catcggcaaa ggaatagccg gcgccgtaca gcgtctcgat cgcggcaaac tcgcggtcga
     1081 cgctgcggaa cttgcggcgc aggcgcttga tgtggctgtc gatcgtgcgg tcatcgacga
     1141 agacatcgtc gctgtaggcc gcgtccatca gctggttgcg gctcttgatc acgccgggcc
     1201 gcagggccag cgcctccagg atcaggaatt cggtgacagt cagcgacacc ggctggccgg
     1261 cccagctgac gcggtggcgg gtcgggtcca tttccagccg cccgcgcacg aaggtttcgc
     1321 tccccgcgtc cgggtcgggc tgggcctcgg cctcgccccg caccagttcg gcccggcgca
     1381 ggatagcccg gatccgcgcg accagcagcc gctggctgaa cggcttggcg atgtaatcgt
     1441 ccgcgcccat cgccagaccc agcgcctcgt ccggttcctc gtccttgctg gtcaggaaga
     1501 tcagcggcag ggcgcttttg gcgcgcagct tctgcagcag ctccagcccg tccatccgcg
     1561 gcatcttgat gtcgcagacc accaggtccg gcggattgtc gagcagggcc ttcagcgcgg
     1621 tggccccgtc cgaatagacc cgggtggcaa agccttcggc ctgcaacccg atcgacaccg
     1681 ttgtcaggat gttgcggtcg tcatcgacca gcgcaatcac ctgctgcgcg gaaggaggag
     1741 gggagggctg gttttcggcc atttcagggg tggaactggg gctcaccggc agcagggcag
     1801 gggccgcagg gttagccgct atcggcggtg cgcgcaacgt ggacttgggc cgctgaaagg
     1861 acttcttcac cgctgcttat gcaaacaata atcgggccat ttgacggaat caccgggcag
     1921 ggctatgcgc gcctcgcgac cgctttgcat tcgtatgcgg aagacggccc ggccgggccc
     1981 gaagccgctt cgcgcttcca acggagatga
//
LOCUS       DASUDV010000012         4492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1502501,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000012 DASUDV010000000
VERSION     DASUDV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502501"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2639)
                     /locus_tag="VFV30_00265"
     CDS             complement(<1..2639)
                     /locus_tag="VFV30_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4846548.1"
                     /translation="MPDLSKILAAKAPLTLSSLARGAQPLVLADLARAAKTRAVFIAP
                     DEAAMQAISDSAAFFAPELDVIAFPAWDCLPYDRASPALSVSARRLSALHRLQTKVSG
                     PQLLVTTINAVLQRVATPFRIRENVRQLKPGMEIGRDSLIALLQRQGYSRTDTVADAG
                     EYAVRGSIFDIYPSGLDKGLRLDFFGDELETLRLFDPNTQRSSGVAESHLLLPASEAM
                     LDEDSVKRFRGRYREMFGANATGDPLYQAVSDGRRLAGMEHWLPLFEERLTTLFDHLG
                     AEDTVVIDSAALGAAEERLGDIADYFAARSEAGGKAAGSYRPLAPAALYLTREEFDER
                     LKGHPVHKAEIFAQPDSAGSVDFGFTGGRDFAPERARGDNVYEAAAQYLTGQAKRGKK
                     ALIAAYSEGSRARIAAILGEAARPAPVLAETWQEALGLAAKGAPVALVLPLEAGFANA
                     ELDLVTEQDILGDRLVRRKKRRKGADAFLAELAALTPGDLVVHMEHGIGRYIGLQSIP
                     VGQSPHDCVALEYAGGDKLYVPVENIDVLSRYGSDAEFVALDRLGGEGWQKRKARLKE
                     RIRQIAHELLKTAALRALRTAPVLVPDDASFGQFVDRFPWTETEDQEAAIDDVLKDLS
                     EGKPMDRLVCGDVGFGKTEVALRAAFVAAMAGQQVAVIAPTTLLARQHFTGFTERFAG
                     FPLKVGRLSRLVGDKEAKATKQGLADGTVDVVIGTHALLSKSLQFKRLGLVIVDEEQR
                     FGVNHKEALKQLRNNVHVLTLTATPIPRTLQMAMSGLRELSTIQTAPVDRLAVRTYVM
                     EWDDMVMREALLREHHRGGQSFIVVPRIADMAEVEDWLHKTVPEVRYISAHGQMAAGE
                     VEERMSAFYEKKYEVLLSTTIVE"
     gene            complement(2756..3022)
                     /locus_tag="VFV30_00270"
     CDS             complement(2756..3022)
                     /locus_tag="VFV30_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX4846549.1"
                     /translation="MVHEDRLRRCKFRAWHRGTREADYMIGCFFDRYGAEWGEAEIAW
                     FEKLLDEEDVDIMGWALGTLSVPDEYAGPLMDAFRKLDFVDIPR"
     gene            complement(3062..3466)
                     /locus_tag="VFV30_00275"
     CDS             complement(3062..3466)
                     /locus_tag="VFV30_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX4846550.1"
                     /translation="MLLPHGTVIALIDGHNFTLYRNAGDEAAPELSALPEPRLDAHNH
                     SGAGHRSSPGNHADSQVNEDAHAGAAVAWLNAQVLDHGIADLVIIAPPRTLGELRRHY
                     HKQTEQVLRKELGKDLIGKQPDAILAALREKH"
     gene            3586..>4492
                     /locus_tag="VFV30_00280"
     CDS             3586..>4492
                     /locus_tag="VFV30_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX4846551.1"
                     /translation="MRPDLLNPLFVAADSLKGVGTGLARPLERLGLTRVRDFAYHLPD
                     RFVTRRAVADLDEASVGENVVVALTPVEYRSSAGRGPFRVLATDAPGNICAITYFGRN
                     TGWAKKALPLGEKRWIAGRLDQYGQMLQIVHPDHVAEESGAALGQLVEPVYPLSEGLT
                     QGRLAALIHQALERVPDLPEWIEPALADRMHWPRWRDALELAHRGEHAAARDRLAYDE
                     LLANSLALLLVKNANRARIGTPLVGDGSLRGKLSLPFGLTGAQSRSVREIEEDMAQAS
                     PMLRLLQGDVGSGKTVVALQAMLLAV"
BASE COUNT          654 a         1504 c         1587 g          747 t
ORIGIN      
        1 ctttcgacga tcgtggtcga cagcagcact tcgtacttct tctcgtagaa ggcgctcatc
       61 cgctcttcca cttcgcccgc cgccatctgg ccgtgggcgg aaatgtagcg gacctcgggc
      121 acggtcttgt gcagccagtc ctcgacctcg gccatgtcgg cgatgcgggg gacgacgatg
      181 aagctctgcc cgccgcggtg gtgttcgcgc agcagggctt cgcgcatcac catgtcgtcc
      241 cattccatca cgtaggtccg caccgccagg cggtcgaccg gggcggtctg gatggtggac
      301 agctcgcgca gcccgctcat cgccatctgc agcgtgcgcg ggatcggcgt ggcggtgagg
      361 gtgaggacgt ggacgttgtt gcgcagctgc ttcagcgctt ccttgtggtt gacgccaaag
      421 cgctgctctt cgtcaacgat tacaaggcca aggcgcttga actgcaggct ctttgacagg
      481 agcgcatggg tgccgatcac cacatcgacc gtgccgtcgg ccaggccctg cttggtcgcc
      541 ttggcctcct tgtcgccaac caggcgggac agccgcccga ctttcagcgg gaagccggcg
      601 aagcgctcgg taaagccggt gaagtgctgg cgcgcgagca gggtggtggg ggcgatcacc
      661 gccacctgct gacctgccat cgccgcgacg aaggcggcgc gcagcgcgac ctcggtcttg
      721 ccaaagccca cgtcgccgca gaccagccgg tccatcggct tgccttcgga gaggtccttc
      781 agaacgtcgt cgatcgccgc ttcctggtct tcggtttcgg tccaggggaa gcggtcgaca
      841 aactggccaa agctggcatc gtccggcacc agcaccgggg ccgtgcgcag cgcgcgcagg
      901 gccgcggtct tgagcagctc gtgcgcgatc tggcggatcc gctctttcag gcgggctttc
      961 cgcttttgcc agccttcgcc gcccagccgg tccagcgcga cgaactccgc gtccgagccg
     1021 tagcgcgaga ggacgtcgat gttctcgacc ggaacgtaga gcttgtcgcc gccggcgtat
     1081 tccagcgcca cgcaatcgtg ggggctttgc ccgaccggga tcgattggag gccgatgtag
     1141 cggccgatcc cgtgctccat gtggaccacc aggtcgcccg gggtcagcgc ggccagctcg
     1201 gccaggaagg cgtcggcgcc cttgcggcgc ttcttgcggc ggaccagccg gtcccccagg
     1261 atgtcctgct cggtcaccag gtcgagctcg gcgttggcaa agccggcttc gagcggcagg
     1321 accagcgcca ccggcgcgcc cttggccgca aggcccaggg cttcctgcca ggtctcggcc
     1381 aggaccgggg cggggcgtgc ggcctcgccc aggatcgcgg cgatgcgggc gcggctgcct
     1441 tcggaatagg ccgcgatcag ggccttcttg ccgcgcttgg cctggccggt caggtattgg
     1501 gcggcggctt cgtagacgtt gtcgccgcgg gcccgctccg gcgcgaaatc gcgcccgccg
     1561 gtgaagccga aatcgaccga tccggcgctg tccggctggg caaagatctc cgccttgtgg
     1621 accggatggc ctttcaggcg ctcgtcgaat tcctcgcggg tcaggtagag ggcggccggg
     1681 gccagcgggc ggtacgatcc cgcggccttg ccgccggcct cgctgcgcgc ggcgaagtaa
     1741 tcggcaatgt cgcccagccg ctcttccgcc gcgcccaggg ccgcgctgtc gatcaccacg
     1801 gtgtcttccg cacccagatg gtcgaacagg gtggtcagcc gctcctcgaa cagcggcagc
     1861 cagtgctcca tcccggccag ccgccgcccg tcgctgaccg cctggtagag cggatcgccg
     1921 gtggcattgg cgccgaacat ctcgcggtag cggccgcgga accgcttgac gctgtcctcg
     1981 tccagcatgg cctcgctggc gggcagaagc aggtggcttt ccgccacgcc gctggagcgc
     2041 tgggtgttgg gatcgaacag ccgcagcgtt tccagctcgt ccccgaagaa gtcgagccgc
     2101 agccccttgt cgaggccgga gggatagatg tcgaagatcg agccgcgcac cgcgtattcg
     2161 ccggcgtcgg ccaccgtatc ggtgcggctg tagccctggc gctggagcag ggcgatcagg
     2221 ctgtcccggc cgatttccat ccccggcttc agctgccgca cgttctcgcg gatccggaag
     2281 ggggtggcga cccgctgcaa gacggcgttg atcgtggtga ccagcagctg cggcccgctg
     2341 accttggtct gcagccggtg cagcgccgac agccgccggg cgctgaccga cagggccggg
     2401 ctggcccggt catagggcag gcaatcccac gccggaaagg cgatgacgtc gagctccggc
     2461 gcgaagaagg cggcgctgtc ggaaatcgcc tgcatcgccg cttcgtccgg ggcgatgaac
     2521 acggcgcggg tctttgcggc cctggccaga tccgccagca ccagcggctg tgcgccccga
     2581 gccagcgagg agagcgtcag cggagccttg gcggccagga ttttcgacag gtcgggcatg
     2641 gttcacacaa cagcgattgc cccgcgggcc attttcgcgg ccagcggggt ggggattcgg
     2701 accgccgcct tagcggcccg tttccgaatt gtcgaacaac cccaaagggt atggttcagc
     2761 gggggatgtc cacaaagtcc agcttgcgga acgcgtccat cagcgggccg gcgtattcat
     2821 ccggcaccga aagagtgccc agcgcccagc ccatgatgtc gacgtcttcc tcgtccagca
     2881 gcttttcgaa ccaggcgatc tccgcttcgc cccattcggc gccatagcgg tcaaagaagc
     2941 agccgatcat gtaatcggct tcgcgcgtgc cgcggtgcca ggcgcggaac ttgcagcggc
     3001 ggaggcggtc ttcgtgaacc atgcccccgc cgctaggctg cggcggcgcc aaaggcaacg
     3061 ctcaatgctt ctcgcgcagt gcggcaagga tcgcgtcggg ctgcttgccg atcaggtcct
     3121 tgcccagttc cttgcgcagc acctgctcgg tctgcttgtg gtaatgccgg cgcagctcgc
     3181 ccagggtgcg gggcggggca atgatcacca ggtcggcgat tccgtggtcc agcacctgcg
     3241 cgttgagcca ggccaccgcc gcgcccgcgt gggcgtcttc gttcacttgg gaatcggcat
     3301 ggttgcccgg gctggagcgg tggccggcac cggaatggtt gtgcgcatcc agccggggtt
     3361 cgggcagcgc gctcagctcc ggggcggcct cgtccccggc gttgcgatag agcgtgaagt
     3421 tgtggccatc gatcagcgcg atgacggttc catggggcag cagcatccgc ggtctccttt
     3481 gtgttgcctg ttggggcaag cacagcgcgg aacggccggc ccgtccatgc gctgagtcaa
     3541 agtccggccc tttcgccgcg ccgccgcttg gggcatgagg gggcgatgcg gcccgacctg
     3601 ctcaatcccc tgttcgtggc ggccgacagc ctgaaaggcg tcggcacggg ccttgcgcgc
     3661 ccgctcgagc ggttggggct gacccgggtg cgggactttg cctatcactt gcccgatcgc
     3721 ttcgtgaccc gccgcgccgt ggccgatctg gacgaggcca gcgttggcga aaacgtggtg
     3781 gtggcgctga ccccggtgga ataccgcagc tcggccgggc gggggccgtt ccgggtgctt
     3841 gccaccgacg cgcccggcaa catctgcgcg atcacctatt tcgggcggaa caccggctgg
     3901 gcgaaaaagg cgctcccctt gggcgagaag cgttggatcg ccgggcggct cgaccagtac
     3961 gggcagatgc tgcagatcgt ccacccggac cacgtggccg aggaaagcgg cgccgcgctg
     4021 ggtcagctgg ttgagccggt ctatccgctg tccgaagggc tcacccaggg acggctggcc
     4081 gcgctgatcc accaagcgct ggaacgggtg ccggacctgc cggaatggat tgagccggcg
     4141 ctggccgacc ggatgcactg gccgcgctgg cgcgacgcgc tggaactggc ccaccgcggc
     4201 gaacacgccg ccgcgcggga ccggctggcc tatgacgagc tgctggccaa cagcctggcg
     4261 ctgctgctgg tcaagaacgc caaccgcgcc cggatcggca cgccgctggt gggcgacgga
     4321 tcgctgcgcg gcaagctcag cctgccgttt ggcctcaccg gcgcgcaaag ccgttcggtg
     4381 cgcgagatcg aggaagacat ggcccaggcc agcccgatgc tgcggctgct gcagggcgat
     4441 gtcggttcgg gcaagacggt ggtggccttg caggcgatgc tgctggcggt gg
//
LOCUS       DASUDV010000013         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1900167,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000013 DASUDV010000000
VERSION     DASUDV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900167"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFV30_00285"
     CDS             complement(<1..706)
                     /locus_tag="VFV30_00285"
                     /inference="COORDINATES: protein motif:HMM:NF023240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3333 domain-containing protein"
                     /protein_id="HEX4846552.1"
                     /translation="MNSPAPEVAQRTPTDWNAPEREARLKARYASERRFRRLGFSAVW
                     TSALFLAVLLWSMTASSIGGFKRAELAVPLDFAALGFNFDPARLASDDAQQALSAARL
                     DDAVALAAVRAFGDDGAALISESGWTTARDALIADPALLSRKAVLWIPASDDLAAAGK
                     GEGTPELRARYAALEAKGQVADRFDPGFMTRSDATDPGQVGIWGALKGSILTMLVTLA
                     LAFPIGVLAALYLEEYA"
     gene            complement(699..2090)
                     /gene="pstC"
                     /locus_tag="VFV30_00290"
     CDS             complement(699..2090)
                     /gene="pstC"
                     /locus_tag="VFV30_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX4846553.1"
                     /translation="MVTAPVLFLLVFGLGLIAWLAGRARAWTFRKSAAGKGAVHSLPA
                     YHGWYAALWAAVPAFVFAAVWSSIAPNLAMQQVLAGPAAAALPVMDMERGAILAEALS
                     VATGAQYGAFNPIAQQLAGPIKQALAHYGTIGAVLTAALGLIGGTFGLLRLRPDFTAR
                     TRVERVVMGFLLIASLVAILTTLGIVASLVFEASRFFQIVSPADFLFGTHWSPDPTAA
                     PGADTGETYGAIPLFWGTLFIGAIIAMVVAIPLGLMSAIYLTQYASPRVRRWMKPLLE
                     ILAGIPTVVYGYFAALTVAPLVRDFASAIGIANPSTESALAAGLVMGVMIIPFVSSMA
                     DDSIAAVPQAMRDGSLAMGATTSETIKKVLVPAALPGIVGGVMLAVSRAIGETMIVVM
                     AAGAAANLTANPFESMTTVTYQIVQMLTGEQSFDHPATLSAFALGLVLFLVTLLLNII
                     ALRVVKRYREAYE"
BASE COUNT          417 a          735 c          737 g          315 t
ORIGIN      
        1 gcgcgtattc ttccagatag agcgcggcga gcacgccgat cgggaaggcc agcgccagcg
       61 tcaccagcat ggtcaggatc gagcccttga gcgcgcccca gatccccacc tgccccggat
      121 cagtggcatc cgaacgggtc atgaagccgg gatcgaaacg gtcggcaacc tggcccttcg
      181 cctcgagcgc ggcatagcgc gcgcgcagct cgggagtgcc ctcacccttg ccagcggcgg
      241 ccagatcatc ggatgccggg atccacagca ccgccttgcg cgacagcagc gcaggatcgg
      301 cgatcagcgc gtcgcgcgcc gtggtccacc cgctttcgga aatcagcgcg gcgccatcat
      361 cgccaaaggc gcggaccgcg gccaaggcaa cggcatcgtc gagccgggct gcggagagcg
      421 cctgctgggc atcgtccgat gccagccggg ccggatcgaa attgaagcca agcgcggcga
      481 aatcgagcgg aacggccagt tcggcgcgct tgaacccgcc gatcgaactg gcggtcatcg
      541 accacagcag caccgccagg aacagcgccg aggtccacac cgcggaaaag ccaagccggc
      601 gaaaacggcg ttcggacgca tagcgcgcct tcagccgggc ttcgcgttca ggcgcgttcc
      661 agtcggtcgg ggtgcgctgg gccacttcgg gcgcgggact attcataggc ttctcggtaa
      721 cgcttgacca cgcgcagcgc gatgatgttg agcagcaggg taaccaggaa cagcaccagc
      781 cccagggcaa aggccgaaag cgtggccgga tggtcgaagc tctgttcgcc ggtcagcatc
      841 tggacgatct ggtaagtcac cgtcgtcatg ctttcgaacg ggttcgcggt aaggttggcg
      901 gcggcgcctg cggccatgac cacgatcatg gtttcgccga tcgcgcggct gaccgccagc
      961 atcaccccgc cgacgatccc gggtagagcg gccggaacca gcaccttctt gatcgtctcc
     1021 gaagtggtcg cccccatcgc caggctgcca tcgcgcatcg cctggggcac tgccgcgatc
     1081 gaatcgtcgg ccatcgagga gacgaacggg atgatcatca cccccatcac cagccctgcg
     1141 gccagcgcgc tttcggtgga gggattggcg atgccgatcg cgcttgcgaa atcgcgcacc
     1201 agcggcgcca cggtgagcgc ggcgaaatag ccgtagacca cggtcgggat cccggcgagg
     1261 atttcgagca gcggcttcat ccagcgccgc acccggggcg aggcatattg ggtgaggtag
     1321 atcgcgctca tcaggccgag cgggatcgcc acgaccatgg cgatgatcgc cccgatgaac
     1381 agcgttcccc agaacagcgg gatcgcgcca taggtttcgc cggtatcggc ccctggcgcc
     1441 gcggtcggat cgggcgacca gtgggttccg aacaggaaat cggccggcga tacgatctgg
     1501 aagaaccggc tggcttcgaa caccagcgag gcgacgatgc ccagcgtggt caggattgcg
     1561 acgagcgagg cgatcagcag gaatcccatg acgacgcgtt caacccgcgt gcgcgcggtg
     1621 aaatcggggc gcaaccgcaa aagcccgaac gtgccgccga tcaggcccaa tgccgcggtc
     1681 agcaccgcgc cgatcgtgcc atagtgcgcc agtgcctgct tgatcggccc ggcaagttgc
     1741 tgggcgatcg ggttgaaggc gccatattgc gcgccagtgg ccacgctcag cgcttcggcc
     1801 aggatcgcgc cgcgttccat gtccatcacc ggcagcgcgg cggcggcggg cccggccaga
     1861 acctgctgca tcgcgaggtt gggcgcgatc gacgaccaca ccgcggcaaa gacgaaggcc
     1921 ggcaccgcgg cccacagcgc cgcataccag ccatggtagg ccggcagcga atgcaccgct
     1981 cccttgcccg cggcggactt gcggaaagtc caggcgcggg cgcgtccagc cagccaggca
     2041 atcaggccca ggccgaaaac cagcaggaaa aggacgggag cggtaaccat tcttgcggcc
     2101 tggccttact tcaactggct cggatcgagc agggtcattt ccgcggcaac cttggcattg
     2161 gcattgcgca cgtcttcggg tgcgatcacc atgccctggc gctt
//
LOCUS       DASUDV010000014         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1988556,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000014 DASUDV010000000
VERSION     DASUDV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988556"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFV30_00295"
     CDS             <1..525
                     /locus_tag="VFV30_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4846554.1"
                     /translation="AGLILSAPMLGFTAGPLPDAVMHQAAKLMKALGDPRRPAWKWSE
                     KPGQVPADRIHLLTHDAERYADELYWREARPELVMEPGSWGWVERAYASMRLLASPGL
                     LEGVTVPVLILATDNDKLVGYGAIARAAARLRDCELVHFGKEAHHEILREGDAVRGRA
                     LAACDTFLARFAAA"
     gene            532..1617
                     /locus_tag="VFV30_00300"
     CDS             532..1617
                     /locus_tag="VFV30_00300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4846555.1"
                     /translation="MVERFDFAVVGAGIAGASLAAGLAGHGRVVLLEAEDRPGHHATG
                     RSAAFWTESYGGPGVLPLTAASGPFLERHGLLTRRGALTLARAEEADGLDAFANEFAA
                     LGVRIERLDRAALLRRLPGLRPDWCLGVLEPDCCDIDVAALHQLWLTEARRGGVELWC
                     RARLDRAEPEAGGWRIALEDGRELSAGVVINAAGAWADAVAARCGAQPLGLQPLRRTM
                     AQLDCGDRVPADLPLTLGFDGSFYFKPEAGKLWLSPHDEIPSPPCDAAPEELDVAMAI
                     DRFEKVVDWPVQRLEHKWAGLRTFAPDRLPVYGFDRTAQGFFWFAGQGGFGIQTAPAA
                     ADLALGLLLGRAPGAVDPAAYDPGRFR"
     gene            1671..1853
                     /locus_tag="VFV30_00305"
     CDS             1671..1853
                     /locus_tag="VFV30_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HEX4846556.1"
                     /translation="MAHRFEIRKNKAGEFVAYFCYNSETIFWTEGYSSKASAKNAIES
                     IKKNGPGADTVDTTAD"
     gene            complement(1997..2431)
                     /gene="rnhA"
                     /locus_tag="VFV30_00310"
     CDS             complement(1997..2431)
                     /gene="rnhA"
                     /locus_tag="VFV30_00310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX4846557.1"
                     /translation="MKTVAIFTDGACKGNPGPGGWGAILRMGHHEKELSGGDPATTNN
                     RMELTAVIRALEALKEPCLVHLHTDSRYVIDGITGWIYGWQKNGWKTAAKKPVLNADL
                     WQDLLAATKRHRIEWIWVKGHDGHPENERADKLASDAALAAA"
     gene            complement(2428..3411)
                     /gene="thrB"
                     /locus_tag="VFV30_00315"
     CDS             complement(2428..3411)
                     /gene="thrB"
                     /locus_tag="VFV30_00315"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX4846558.1"
                     /translation="MAVYTHLGAEDLAALIAHFDVGTLTSAKGIAEGVSNSNWLVETT
                     GRDGAGAKFILTMYEFRIEVEDLPFFLSLLDHLAARGCPVPRTIHDRDGALFRMIDGK
                     AAALIEFLPGVSVSHPTPGQARAVGRALAQLHLASADFPAARANAMDLAESRRLAEAC
                     GHAGMVAIDPALADLVARELPLLERAWPADLPRSVIHADLFPDNVLMLGDTVTGLIDF
                     YFACNDVTAYDMAVTHAAWCFDGEGRNFSPAISAALLEGYEAVRPLSAEERAALPVLT
                     RAAALRFTMSRAFDWLNTPADALVVRKSPMAFARRLEFYADPANAALFDRP"
     gene            complement(3411..4373)
                     /gene="ispH"
                     /locus_tag="VFV30_00320"
                     /gene_synonym="lytB"
     CDS             complement(3411..4373)
                     /gene="ispH"
                     /locus_tag="VFV30_00320"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX4846559.1"
                     /translation="MNAPFNPAEKPPLRLLLAAPRGFCAGVDRAIDIVELALQRYGAP
                     VYVRHEIVHNRFVVDSLKAKGAVFVEELDEVPSGVPVVFSAHGVPKVVPAEADQRGLD
                     WLDATCPLVSKVHRQAERQIEAGRHIVFIGHAGHPEVIGTMGQVPEGAITLVETVEDV
                     EALSFTDETPLAFLTQTTLSVDDTAEVIGALRARFPHIVGPKAEDICYATSNRQAAVK
                     AIAPECQLVLVIGAPNSSNSLRLVEVAQRCGARGMLVQRGAEIEPQWLDGVEVLGLTA
                     GASAPEELVNEVIARIAEWREVTVEQVVTAEEKMVFKLPRQLVD"
BASE COUNT          648 a         1484 c         1573 g          684 t
ORIGIN      
        1 gccgggctga tcctgtccgc gccgatgctg ggctttaccg ccgggccgct gcccgatgcg
       61 gtgatgcacc aggcggccaa gctgatgaag gcgctgggcg atccccgccg cccggcctgg
      121 aagtggagcg agaagcccgg gcaagtcccg gccgaccgga tccacctttt gacccacgat
      181 gccgagcggt acgccgacga gctctactgg cgcgaggcgc ggcccgaact ggtgatggag
      241 ccgggatcgt ggggctgggt cgagcgggcc tatgcctcga tgcggctgct cgcctcgccg
      301 gggctgctgg agggcgtgac cgtgccggtg ctgatcctgg cgaccgacaa cgacaagctg
      361 gtcggctatg gcgcgatcgc ccgcgcggcg gcacggctgc gcgactgcga gctggtccac
      421 ttcggcaagg aagcccacca cgagatcctg cgcgaagggg acgcggtgcg cggccgggcg
      481 ctggccgcct gcgacacctt tctggcgcgc ttcgccgcgg cctgaccggt catggtggag
      541 cggttcgatt ttgcggttgt cggggccggg atcgccggag cctcgctggc ggccgggctg
      601 gcgggccacg ggcgggtggt gctgctggag gcggaggacc gccccggcca ccacgccacc
      661 ggccgttccg ccgcgttctg gaccgaaagc tatggcggcc ccggggtcct gccgctcacg
      721 gccgcctcgg gtccgttcct ggagcgccac gggctgctga cccggcgcgg cgcgctgacc
      781 ctggcccggg ccgaggaggc cgatgggctg gacgcctttg cgaacgagtt cgccgccctg
      841 ggggtgcgga tcgaacggct ggaccgggcc gcgctgctgc ggcggctgcc gggcctgcgc
      901 ccggactggt gcctgggcgt gctggaaccc gattgctgtg acatcgatgt cgccgcgctg
      961 caccagctgt ggctgaccga ggcccggcgc ggcggggtgg agctgtggtg ccgcgcccgg
     1021 ctggaccggg ccgaaccgga agccggcggc tggcggatcg cgctggagga cgggcgggag
     1081 ctttccgccg gggtggtgat caacgccgcc ggggcctggg ccgacgcggt tgccgcgcgc
     1141 tgcggagcgc agccgctggg cctccagccg ctgcgccgga ccatggccca gctcgattgc
     1201 ggggaccggg ttccggccga tctgccgctg accctgggtt ttgacggctc gttctatttc
     1261 aagcccgagg ccggcaagct gtggctgagc ccgcacgacg aaatccccag cccgccctgc
     1321 gatgcggcgc ccgaggaact ggacgtggcc atggcgatcg accggttcga aaaggtggtc
     1381 gattggccgg tgcagcggct ggagcacaag tgggccggcc tgcgcacttt cgcgccggac
     1441 cggctgccgg tctatggctt cgatcggacc gcgcaggggt tcttctggtt tgccgggcag
     1501 ggcggcttcg ggatccagac cgcgccggcg gcagccgacc tggcgctggg gctgctgctg
     1561 ggccgcgcgc ccggcgcggt cgatccggcg gcctatgatc cgggccgatt ccgctagcga
     1621 atcgctgcaa tcagagtaac ttgggcggca cccagcaacg gaggttcccc atggctcacc
     1681 gcttcgaaat ccgcaaaaac aaggccggcg agttcgtcgc ctacttctgc tacaacagcg
     1741 aaacgatctt ctggaccgaa ggctattcca gcaaggccag cgccaagaac gcgattgaat
     1801 cgatcaagaa gaacggcccc ggcgccgata cggtcgatac cacggccgac tgatcgatcc
     1861 tccccgaaac ggggaggggg accatgcgca gcatggtgga ggggcacagg cgggctcgcc
     1921 ggacactcaa ggtcaggtgc agcggcccgt gcccctccac cagcttcgct ggtccccctc
     1981 ccctaagagg gaggaattac gccgcggcca gcgcggcgtc gctggccagc ttgtcggccc
     2041 gctcgttttc cgggtggccg tcgtggcctt tcacccagat ccattcgatc cggtgccgct
     2101 tggtcgcggc cagcaggtcc tgccacagat cggcgttgag gaccggcttc ttcgcggcgg
     2161 ttttccaacc gttcttctgc cagccgtaaa tccagccggt gatcccgtcg atcacatagc
     2221 ggctgtcggt gtggaggtgg accaggcacg gctccttcag cgcttccagc gcgcggatca
     2281 ccgcggtcag ctccatccgg ttgttggtgg tggcgggatc gccgccggac agttcctttt
     2341 cgtggtggcc catgcgcagg atcgcgcccc agccgccggg gccggggttg cccttgcagg
     2401 ctccgtcggt gaaaatcgcg accgtcttca cggccggtca aacagcgcgg cattggccgg
     2461 atcggcatag aattccagcc gccgggcaaa ggccatcggg ctcttgcgca ccaccagcgc
     2521 gtcggccggc gtgttgagcc agtcaaaggc gcggctcatc gtgaagcgca gcgcggcggc
     2581 gcgggtcagc accggcaggg cggcacgctc ctcggccgag agcgggcgga ctgcttcgta
     2641 gccttccagc agcgcggcgg agatcgccgg gctgaaattg cggccttcgc catcaaagca
     2701 ccaggccgcg tgagtcaccg ccatgtcgta ggcggtgaca tcgttgcagg cgaagtagaa
     2761 gtcgatcagg ccggtgaccg tgtcccccag catcagcacg ttgtccggga acaggtcggc
     2821 gtggatgacc gagcggggca gatcggccgg ccaggcccgt tccagcagcg gcagttcgcg
     2881 cgcgaccaga tcggccaggg ccggatcgat cgcgaccatt ccggcgtgcc cgcaggcctc
     2941 ggccagccgg cggctttcgg ccaggtccat cgcgttggcg cgggcggctg ggaaatcggc
     3001 gctggccagg tgcagctggg ccaacgcccg gcccaccgcg cgggcctggc ccggggtggg
     3061 atggctgacc gagacgcccg gcaggaattc gatcagcgcc gccgccttgc catcgatcat
     3121 ccggaacagc gcgccgtccc ggtcgtggat cgtgcgcgga accgggcagc ccctggccgc
     3181 caggtgatcg agcagcgaga ggaagaacgg caggtcctcc acctcgatgc ggaattcgta
     3241 catcgtcagg atgaacttcg cgccggcgcc gtcccgtccg gtggtttcga ccagccagtt
     3301 ggaattggac acgccttcgg caatgccctt ggcgctggtc agcgtgccga catcgaaatg
     3361 ggcaatcagc gcggccaggt cctcggcccc aaggtgggtg tagaccgcca tcagtcgacg
     3421 agctggcggg gcagcttgaa gaccatcttc tcctcggcgg tgacgacctg ttcgaccgtc
     3481 acctcgcgcc attcggcaat ccgggcgatc acttcgttga ccagctcttc gggggccgat
     3541 gccccggcgg tcaggcccag cacctccacc ccgtcaagcc actgcggctc gatctccgcg
     3601 ccccgctgga ccagcatccc gcgcgcgccg cagcgctggg ccacttcgac caggcgcagc
     3661 gagttggagc tgttgggcgc accgatcacc agcaccagct ggcattcggg cgcaatcgcc
     3721 ttcaccgcgg cctggcggtt cgacgtggca tagcagatgt cctcggcctt gggcccgacg
     3781 atatggggaa agcgcgcgcg cagcgcgccg atcacttcgg ccgtgtcgtc gacggaaagc
     3841 gtggtctggg tcaggaaggc cagcggggtt tcgtccgtaa aggacagcgc ttcgacatcc
     3901 tccaccgttt cgaccagcgt gatcgcgccc tcgggcacct ggcccatggt gccgatcact
     3961 tcgggatggc cggcatggcc gatgaacacg atgtggcgcc cggcctcgat ctggcgttcg
     4021 gcctggcggt ggaccttgga gaccagcggg caggtggcat cgagccagtc cagaccgcgc
     4081 tggtcggcct cggccgggac caccttgggc acgccgtggg cgctgaacac caccggcacg
     4141 ccggacggca cctcgtccag ctcttccacg aaaaccgcgc ccttggcctt caggctgtcg
     4201 accacgaagc ggttgtggac gatctcgtgc cggacataga ccggggcacc atagcgctgc
     4261 agcgccagtt cgacgatgtc gatcgcccgg tccaccccgg cacagaaacc gcgcggggcg
     4321 gccagcagca gacggagcgg gggcttttcg gcgggattaa agggcgcgtt catgttggcc
     4381 ccactagcg
//
LOCUS       DASUDV010000015         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_110934,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000015 DASUDV010000000
VERSION     DASUDV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110934"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFV30_00325"
     CDS             complement(<1..470)
                     /locus_tag="VFV30_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX4846560.1"
                     /translation="MASSDTTESEGDGQSGHYIDSHGHHHGGLIALAVGAIGVVFGDI
                     GTSPLYAFRETFASHHGVPGIQVDPVHIHGVLSLVFWSMMIVVTFKYVLTIMKADNKG
                     EGGSLALLALINRKSESAKWTGPFVLLGVFATALFYGDSMITPAMSVLSATEGL"
     gene            complement(550..1191)
                     /locus_tag="VFV30_00330"
     CDS             complement(550..1191)
                     /locus_tag="VFV30_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4846561.1"
                     /translation="MTWVMVIGLALVAFGLLTWAFKAPRQGREAIAAALLVGMAGFAS
                     QAATELPGAPKEPAQQAEKSGAALIEARRQLAGQTATPNAPNRWMVIADALSRNGQFG
                     DAAGIVLGAVEQNPQDADAWLSLANNLVAHAEGNLTPAAQYAYARALQANPDHPGPPF
                     FLGLALAGSGKLAEGRAQWAALLERTPPDAPWRADLVERLKRLDAFIAMQGQR"
     gene            complement(1188..1601)
                     /locus_tag="VFV30_00335"
     CDS             complement(1188..1601)
                     /locus_tag="VFV30_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX4846562.1"
                     /translation="MSRPLILALALLLAGPALADSKMPAAPYANVQLEDPAAEARAKA
                     LMETLRCLQCQGQSISDSDAQIAGDMRSQVRLRIKAGEDPEAIRAWLVERYGDYVSFA
                     PRVTSATWPLFVAPLALLALALLLVRRRFFGGNKP"
     gene            complement(1598..2155)
                     /locus_tag="VFV30_00340"
     CDS             complement(1598..2155)
                     /locus_tag="VFV30_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX4846563.1"
                     /translation="MSDPQTPPRTSSWALWLPALFFAGFVAIVAYNLRNPAERVVRSA
                     LVGKPMPEFTLQPALEGQPGLTRADLANGKVHLVNIFASWCIPCAVEAPQLAALKAQG
                     VEVVGIAIRDRPEDLKAFLARHGNPFGRIAADNTSAIQFALGSSGVPESFVVDGKGVI
                     RYQHIGEIRPEHVPMVLDQVREAGE"
     gene            complement(2152..4095)
                     /locus_tag="VFV30_00345"
     CDS             complement(2152..4095)
                     /locus_tag="VFV30_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX4846564.1"
                     /translation="MIANFGLAALWFAAALAALQLLAGGLALTRSGAGLAGLVRPAAV
                     VQGILALLAFLALIVAFLQTDLSVQLVVKNSHSAKPLLYKFAGTWGNHEGSMLLWVMV
                     MGLAGGFLALVERRLPEPTMLATLAAQAFVSLGFYAFLLFASNPFARSNPVPAEGMGL
                     NPLLQDPGLAFHPPTLYIGYVGLSVAFSFAVGALLTRQVGPAFARAMRPWVLGAWIFL
                     TIGITAGSYWAYYELGWGGWWFWDPVENASLMPWLAATALLHSASVLASRDALRAWTV
                     MLGVVAFSMSMVGTFLVRSGILTSVHAFAVDPTRGAFILVLLGIYIGGALNLFAIRAS
                     TINEGKQFTVTSREGALVFNNVMLSSILGIVLIGTLYPLLAETLGAQVSVGPPYFNPM
                     SAVFTVPMLIVLAVGPILRWRRDRMERVRPVLILPAVVTLVSGAMCAVAGLALLPALG
                     IALAMGLAFATLLPLRGRRLRTVTLPLWGMVIAHFGVAVALFGMASDAAFTKEKLVAV
                     APGQTVQVGPWQVTLEAIEPVAGPNWTALNAALGARYDGGKRIELHPQARSFWSPPQE
                     TSEAALATRWNGQLYAVLGERAEDGRWQLRLWWKPFVTLIWFGGLLIALGGALALVGR
                     VLSDVRRGVAQEKIAYRRMRQGR"
     gene            complement(4092..4550)
                     /gene="ccmE"
                     /locus_tag="VFV30_00350"
     CDS             complement(4092..4550)
                     /gene="ccmE"
                     /locus_tag="VFV30_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX4846565.1"
                     /translation="MNGIVKAKHQRLVLVVIALAAVIGAGVLAAWSLRSQASFFYLPA
                     QMAADPPAPDRAVRLGGMVEAGSIKTEADGVTIRFVVGDGKARVPVTFRGILPDLFKE
                     NSGVVAEGKRRADGTFVADNLLAKHDEKYVPREMKDMTEAQARQTVEETR"
     gene            complement(4547..4702)
                     /locus_tag="VFV30_00355"
     CDS             complement(4547..4702)
                     /locus_tag="VFV30_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846566.1"
                     /translation="MYEGLNHWGFVIASYVLTVGGTGALIAASLMAMRRAEARRDAAR
                     DNRKQDR"
     gene            complement(4695..5426)
                     /gene="ccmC"
                     /locus_tag="VFV30_00360"
     CDS             complement(4695..5426)
                     /gene="ccmC"
                     /locus_tag="VFV30_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX4846567.1"
                     /translation="MHGFANPARFLRLARLLTGPLLIAGLLLTGASLLWGLFGAPAER
                     LQGDSVRILFVHVPAAWLGMGGWTAIAIASLTELVWRHPLAGIAGRAIAVPGAFFALL
                     CLVTGSLWGRPAWGTWWVWDGRLTSMLVLFFLYCAYIALADAANRDGESGSRLPAIFG
                     LVGAINIPIIHFSVLWWNSLHQPPSISVGKSEMAAPFLYGLLAATIGFSLLFGAVVLM
                     RMRAILADAQAEARLRRRAMEAADV"
BASE COUNT          983 a         1959 c         1823 g          739 t
ORIGIN      
        1 tgcaggcctt ccgtggccga caggaccgac atcgccgggg tgatcatcga atcgccatag
       61 aacagcgcgg tggcgaagac cccgagcagc acgaacggcc cggtccactt ggcgctttcg
      121 gacttgcggt tgatcagcgc cagcagcgcc aggctgccgc cctcgccctt gttgtcggcc
      181 ttcatgatgg tcaggacgta tttgaacgtc accacgatca tcatcgacca gaagaccagg
      241 ctcagcaccc cgtggatgtg caccggatcg acctggatcc ccggcacgcc gtggtgcgag
      301 gcaaaggttt cgcggaacgc gtaaagcggg ctggtgccga tatcgccgaa caccacgccg
      361 atcgcgccga ccgccagcgc gatcagcccg ccatggtgat gcccgtggct gtcgatgtaa
      421 tggccggact ggccatctcc ctccgactcg gtggtgtcag aactcgccat gcaacccgca
      481 acccccttga gtcggcgcaa gaaaccgaaa ttagcacccg ccgatagccc ggtccagcaa
      541 tgccggggcc tagcgctgcc cctgcatagc tatgaaagcg tcaagccgct tcagccgttc
      601 aaccaggtcg gcccgccacg gtgcatcggg cggggtccgc tccagcaggg cggcccactg
      661 cgcccgcccc tcggccagct tgccgctgcc ggccagcgcc aggccgagga agaacggcgg
      721 ccccggatga tcgggattgg cctgcaaggc gcgagcatag gcatactgcg cggccggcgt
      781 caggttgccc tcggcgtgcg cgaccaggtt gttggccagc gacagccagg cgtcggcatc
      841 ctggggattc tgctccaccg cgcccagcac gatgccggcg gcatcgccga actggccgtt
      901 gcgcgacagg gcatcggcga tcaccatcca gcggttcggc gcgttgggcg tggcggtttg
      961 accggccagc tgccgccgcg cctcgatcag cgccgcgccg ctcttttcgg cctgctgggc
     1021 cggctccttg ggcgcgccgg gcagctcggt cgcagcctgc gaggcaaagc cggccatgcc
     1081 caccagcagc gcggcggcaa tcgcctcacg cccctggcgc ggggccttga acgcccaggt
     1141 cagcaacccg aacgcgacca gcgccaggcc gatcaccatc acccaggtca tggcttgttt
     1201 cctccgaaaa agcggcggcg caccagcagc agggccaggg caagcaaggc cagcggggcc
     1261 acgaacagcg gccaggtggc gctggtcacc cgcggcgcga agctgacata gtcgccatag
     1321 cgctccacca gccaggcgcg gatcgcttcg ggatcctcgc cggccttgat ccgcagccgc
     1381 acctggctgc gcatgtcacc ggcgatctgg gcatcggaat cggaaatcga ctggccctgg
     1441 cattgcaggc agcgcagcgt ttccatcagc gccttggccc gcgcctcggc cgccggatcc
     1501 tccagctgga cattggcata gggcgccgcc ggcatcttgg aatccgccag cgccggaccg
     1561 gccagcagca gcgccagggc caggatcagc ggccggctca ttccccggcc tcgcggacct
     1621 gatccagcac catcggcaca tgctcgggcc ggatttcgcc gatgtgctga tagcggatca
     1681 cgcccttgcc atcgacgacg aagctttccg gcacgcccga cgagcccagc gcgaactgga
     1741 tcgcgctggt gttgtcggcg gcgatccggc caaacgggtt gccgtgccgg gcgaggaagg
     1801 ccttgaggtc ctcgggccgg tcgcggatcg cgatgccgac cacttcgacc ccttgcgcct
     1861 tcagcgcggc gagttgcggc gcttcgaccg cgcagggaat gcaccagctg gcgaagatgt
     1921 tgaccaggtg gactttgcca ttggccagat cggcgcgggt caggcccggc tggccttcca
     1981 gcgccggctg cagggtgaat tcgggcatcg gcttgccgac cagcgccgag cgcaccaccc
     2041 gctcggccgg attgcgcagg ttgtaggcga cgatggcgac gaacccggca aagaacaggg
     2101 ccggcagcca cagcgcccag ctggacgtgc gcggaggggt ttgcggatcg ctcatcggcc
     2161 ctgcctcatc cgccggtagg cgatcttttc ctgcgccacg ccgcggcgca cgtcgcttag
     2221 cacgcggccg accagggcca gcgccccgcc cagcgcgatc agcaggccgc cgaaccagat
     2281 cagggtcacg aacggtttcc accacagccg cagctgccag cgcccgtcct cggcccgttc
     2341 gcccagcacg gcataaagct gcccgttcca gcgggtggcc agcgcggcct cgctggtttc
     2401 ctgcggcggc gaccagaagc tgcgggcctg cgggtgcagc tcgatccgct tgcccccgtc
     2461 atagcgggcg cccagcgcgg cgttgagcgc ggtccagttg ggcccggcga ccggttcgat
     2521 cgcttccagc gtcacttgcc acgggccgac ctgcacggtc tgccccgggg ccacggcgac
     2581 cagcttttcc ttggtgaagg cggcgtcaga ggccatgccg aacagcgcca cggccacgcc
     2641 gaagtgcgcg atcaccatgc cccacagcgg cagggttacc gtccgcaggc ggcgcccgcg
     2701 cagcggcagc agcgtggcaa aggccaggcc catggcgagc gcaatgccca acgccggcag
     2761 cagcgccaga ccggccaccg cgcacatcgc gcccgagacc agcgtcacca cagccggcag
     2821 gattagaacc gggcggaccc gctccatccg gtcgcgccgc cagcgcagga tcgggcccac
     2881 cgccagcacg atcagcatcg gcacggtgaa cacggcgctc atcgggttga aatagggcgg
     2941 ccccaccgaa acctgcgcgc ccagcgtttc ggccagcagc ggatagagcg tgccgatcag
     3001 gacgatgcca aggatgctgg acagcatcac gttgttgaac accagcgcac cttcgcggct
     3061 ggtgacggtg aactgcttgc cttcgttgat cgtcgaggcg cggatcgcga acaggttgag
     3121 cgcgccgccg atgtagatcc ccagcagcac caggatgaag gccccgcggg tgggatcgac
     3181 cgcgaaggcg tggaccgacg taagaatgcc ggagcggacc aggaaagtgc cgaccatcga
     3241 catcgaaaag gcaaccacgc ccagcatcac cgtccaggcc cgcaaggcat cgcgcgaggc
     3301 caggaccgaa gccgaatgga gcagcgcggt tgccgccagc cacggcatca ggctcgcgtt
     3361 ctccaccgga tcccagaacc accagccgcc ccagcccagc tcgtagtagg cccagtagga
     3421 tccggcggtg atcccgatcg tcaggaatat ccacgccccc agcacccagg gccgcatcgc
     3481 ccgggcaaag gccgggccga cctgccgggt cagcagcgcg ccgaccgcaa agctgaaggc
     3541 gaccgacagc ccgacatagc cgatgtacag cgtgggcggg tggaaggcga ggcccgggtc
     3601 ctgcagcagc gggttgagcc ccatgccttc ggccggaacc ggattgctgc gcgcaaacgg
     3661 gttcgaggcg aacagcagga atgcgtagaa gccgaggctg acaaaggcct gcgcggccag
     3721 ggtggccagc atggtcggtt cgggcaggcg ccgctcaacc agggcgagga agccgccggc
     3781 caggcccatc accatcaccc acagcagcat cgagccttcg tggttgcccc aggttccggc
     3841 aaacttgtaa agcagcggct tggccgaatg ggagttcttc accaccagct gcaccgacag
     3901 gtcggtctgc aggaaggcca cgatcagggc caggaaggcc agcagcgcca agatcccctg
     3961 aacgaccgcg gccggacgga ccagcccggc cagccctgcg ccgcttcggg tcagggccag
     4021 gccgccggcg agcagttgca gcgccgccag cgccgcggcg aaccacaggg cggccaggcc
     4081 gaaattggcg atcaccgggt ttcctccacg gtctgccggg cctgggcctc ggtcatgtcc
     4141 ttcatttcgc gcggcacgta tttctcgtcg tgcttggcca gcaggttgtc ggccacgaag
     4201 gtcccgtcgg cgcggcgctt gccttcggcg accacgccgg agttttcctt gaacagatcg
     4261 ggcaggatgc cgcggaaggt caccggaacc cgggccttgc catcgccaac cacgaagcgg
     4321 atcgtgaccc cgtccgcctc ggtcttgatc gatccagcct ctaccatgcc gcccagccgc
     4381 acggcgcggt ccggcgcggg cggatcggcc gccatctggg ccggaaggta gaagaacgag
     4441 gcctggctgc gcagcgacca ggcggccagc accccggccc cgatcaccgc ggccagtgcg
     4501 atcaccacca gcaccaggcg ctggtgcttg gccttgacga tcccgttcat cgatcctgct
     4561 ttcggttgtc gcgcgccgcg tcacgccggg cttcggcgcg gcgcatggcc atcaggctgg
     4621 cggcgataag ggcccctgtt ccccccacgg tcaggacgta agacgcgatc acgaagcccc
     4681 agtggttcag gccttcatac atcggcggcc tccatcgcgc ggcggcgcag gcgggcttcg
     4741 gcctgggcat cggccaggat cgcgcgcatc cgcatcagca ccacggcgcc gaaaagcagc
     4801 gaaaatccga tcgttgcggc cagcagccca tagaggaaag gggcggccat ctcgctcttg
     4861 ccgacgctga tcgacggcgg ctggtgcagg ctgttccacc acagcaccga gaaatggatg
     4921 atcgggatgt tgatcgcgcc gaccagcccg aagatcgcgg gcaggcgcga tccgctctcg
     4981 ccatcgcggt tggcggcatc ggccagggcg atataggcgc agtagaggaa gaacagcacc
     5041 agcatgctgg tcaggcgccc gtcccagacc caccaggtgc cccaggccgg acggccccac
     5101 agcgaaccgg tgaccaggca cagcagcgca aagaaggccc ccggcacggc aatcgcccgg
     5161 ccggcaatcc cggccagcgg atggcgccag accagctctg tcaggctggc aatcgcaatc
     5221 gcggtccacc cgcccatgcc cagccaggcg gcgggcacgt ggacgaacag gatgcggacc
     5281 gaatccccct gcaaccgttc cgccggagcg ccaaacaggc cccacagcag cgacgccccc
     5341 gtaaggagca ggcccgcgat caacaaggga ccggtcaaca ggcgcgcaag gcgcagaaac
     5401 cgggcaggat tggcaaagcc gtgcataacc gctttcgtcg attcgaggcc cgttgcggag
     5461 ccgcgcccct ctgccacgcg gcatccctat cggcaagcgg caag
//
LOCUS       DASUDV010000016         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_574914,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000016 DASUDV010000000
VERSION     DASUDV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574914"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1954)
                     /locus_tag="VFV30_00365"
     CDS             complement(83..1954)
                     /locus_tag="VFV30_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4846568.1"
                     /translation="MSEAYQAAWRESIEDREAFWTRAAGAIDWITPPSAAYAEGRGWF
                     PGGTLNTAHNCIDRHVAAGRGAAIALAYDSPVTDTVRRYTYAELQDEVGRVAAMLARL
                     GVAKGDRVIVYMPMIPETVFAMLACARLGAIHSVVFGGFAPLELAKRIDDATPKVLLT
                     ASCGIEGARTIAYKPMVDEALALASHPVEQVVVFQRERLPAELIPERDRDWMELRAEA
                     AADPVPPCAELAADDPLYILYTSGTTGVPKGVVRENGGHAVALAWSMANIYGIGPGDP
                     FWAASDVGWVVGHSYIVYGPLIAGATSVLFEGKPVGTPDPGTFWRVMSRHGVKSFFTA
                     PTAIRAVRKEDPDAVLLKEIGTGDCRVIFLAGERADPDTIAWLEEQSGLPVIDHWWQT
                     ELGWPGVASCFALNDLRRKRGSAGFPVPGYEFAILGEDGQPLPQGQSGAVAIKEPLAP
                     GAFRSLWNNPAGFAKNFATFPGWYETGDAGHFDEEGFLHIMGRTDDIINVAGHRLSTG
                     QMEQIVAQQDGVAECAVIGADDPIKGMIPIAFVIPRAGFEGDGSMAARVLGAVRAELG
                     AVAALKTAYVVPALPKTRSGKILRNLLRKIVNGEEFQTPPTIEDPAVPALIAAAVGR"
     gene            complement(2122..>2302)
                     /locus_tag="VFV30_00370"
     CDS             complement(2122..>2302)
                     /locus_tag="VFV30_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4846569.1"
                     /translation="PYHIGNGWFGGFLPTTAFAMVAATGDIYYGLWYPIVIAGLTVVA
                     GLLFLPETFKRNIDD"
BASE COUNT          350 a          795 c          806 g          351 t
ORIGIN      
        1 gtgcgcgccc gctatgacgc caacggcaag ggcaagggcc cgcgccacgt gatctggttc
       61 gcgcgggcct gagcgggcct ggctagcgcc ccaccgccgc cgcgatcagc gccggaaccg
      121 cgggatcctc gatcgtcggc ggggtctgga attcctcgcc gttgacgatc ttgcgcaaga
      181 ggttgcgcag gatcttgccc gaccgggtct tgggcagggc cgggacgaca taggcggtct
      241 tcagcgcggc gaccgcgccc agctccgccc ggaccgcgcc gagcacccgc gccgccatgc
      301 tgccgtcgcc ctcgaacccc gcgcgcggga tcacgaaggc gatcgggatc atgcccttga
      361 tcggatcgtc ggcgccgatc accgcgcatt cggcgacgcc gtcctgctgg gcgacgatct
      421 gctccatctg cccggtcgag aggcggtgcc cggcgacgtt gatgatgtca tccgtgcggc
      481 ccatgatgtg gaggaaaccc tcctcgtcga agtgcccggc atcgccggtt tcgtaccagc
      541 cggggaaggt ggcgaagttc ttggcaaagc cggccgggtt gttccacagg ctgcggaaag
      601 cgccgggggc cagcggctct ttgatcgcca cggcgccgct ttgcccctgg ggcaggggct
      661 ggccatcctc gcccaggatc gcgaattcat agcccggcac ggggaagccc gcgctgccgc
      721 gcttgcggcg caggtcgttc agggcaaagc agctggccac gccgggccag cccagctcgg
      781 tctgccacca gtggtcgatc accggcaggc cgctctgctc ttccagccag gcgatcgtat
      841 cgggatcggc gcgctccccg gccaggaaga tcacccggca gtccccggtg ccgatctcct
      901 tcagcagcac cgcgtccgga tcctccttgc gcaccgcgcg gatcgcggtg ggggcggtga
      961 agaagctttt gacgccgtgg cggctcatca cccgccagaa ggtgccagga tcgggcgtgc
     1021 cgaccggctt gccttcgaac agcacagagg tcgccccggc gatcagcggg ccgtagacga
     1081 tgtagctgtg gcccacgacc cagcccacgt cgctcgccgc ccagaacgga tcgcccgggc
     1141 cgatgccata gatgttggcc atcgaccagg cgagcgcgac ggcgtggccg ccgttttcgc
     1201 ggaccacgcc cttgggcacg ccggtcgtgc cggaggtgta gaggatgtag agcggatcgt
     1261 ctgcggcgag ctccgcgcag ggcggcacgg gatcggcggc ggcctcggcg cgcagctcca
     1321 tccagtcgcg gtcgcgctcc gggatcagct cggcgggcag gcgctcgcgc tggaacacca
     1381 cgacctgctc gaccggatgg ctggccagcg ccagcgcctc gtccaccatt ggcttgtagg
     1441 caatggtccg cgcgccttcg atcccgcagc tggcggtcag cagcaccttg ggcgtggcat
     1501 cgtcgatccg cttggccagt tccagcgggg cgaagccgcc gaacaccacc gaatggatcg
     1561 cccccagccg cgcgcaggcc agcatggcaa agacggtttc ggggatcatc ggcatgtaga
     1621 cgatcacccg gtcgcccttg gccacgccca ggcgggcgag catggcggcc acccggccaa
     1681 cctcgtcctg cagctcggca taggtgtagc ggcggaccgt gtccgtcacc gggctgtcat
     1741 aggcgagcgc gatagccgcg ccgcggccgg cggccacgtg gcggtcgatg cagttgtggg
     1801 cggtgttgag cgtgccgccc ggaaaccagc cgcgcccttc ggcataggcg gccgatggcg
     1861 gtgtgatcca gtcgatcgcg ccggccgcgc gggtccagaa ggcttcgcgg tcttcgatgc
     1921 tttcgcgcca ggcagcctga taggcttcgc tcatgtcacc cgtccccttt catcatcatc
     1981 ctatcgccgc ggacgggccg gggcgagagc caatcggcag tcaaaacaaa gccggagcga
     2041 gggccgatgc agcatcgccg tcgctccggt tcaaggtttg ggacgcgccg ccagggtgtg
     2101 cggcggcgcg tcccggggag atcaatcgtc gatgttgcgc ttgaaggttt ccggcaggaa
     2161 tagcagcccc gccaccaccg tcaggccggc gatcacgatc gggtaccaca ggccgtagta
     2221 gatatcgccg gtggccgcga ccatcgcgaa ggccgtggtc ggaaggaacc cgccgaacca
     2281 gccgttgccg atgtgatagg gc
//
LOCUS       DASUDV010000017         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1811973,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000017 DASUDV010000000
VERSION     DASUDV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811973"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2505
                     /locus_tag="VFV30_00375"
     CDS             <1..2505
                     /locus_tag="VFV30_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX4846570.1"
                     /translation="ADTNASTGFERTTYMLDLPKNDPELLDVALMLMRETVSELTFSP
                     GAVERERGVVQAEMRDRNTFALRNVKDQLDFAFPGSKVANRLPIGTPESLAGATPEGL
                     KAFWQREYVPEHTTLYVIGDFDPDMVEQKIKDRFGSWKGKAPAEPKPKAGPIAIETQG
                     GEDVYVDPALSERVSVSRKGAWVEEPDSLAQRRENLLRQIAYGIINRRLLRRSREENA
                     PFRNASFGTGNIFKEGRSTELNIDTVDGKWRRGMIEAGLEYRKALTYGFTKEEVDEQL
                     AIIRNAQANAAEGEATRSNAQLLGAAMALVNDERIPDTPRNSLNRLDAFIPEITPDAV
                     VGALRRDMVPLEEPQIRFQGRTPPADGEKGLREAWKQAMTAPIRPNERKAAGNFGYTD
                     FGPPGRVVSDVREPRLGIRTIRFANGVRLNIKRTDLRKDQVLVQVSVDGGDLLATADK
                     PLATRMMSVLSLGGLGKHSKDELDTMLAGKTVSSNYSATDETFVAIASTTPRDLELQL
                     QVLTAQIVDPGYRKEGEVLFRQTINNLFASLRATPAAALSADLGDILSGGDPRFSLGK
                     VEDYRTLTFDQLRGTVSDRFKNGAIEIGIVGDVDEDAAIALVAKTFGALPQREAEFRP
                     YADRRDRPFTKERKRHVLRHTGQKDQAIVTYIWPTRDGEDAAADAELELLERVMRIEM
                     TDTLREKLGKAYSPGASSNTSRTWRNYGTFSVNASVAVAEVPAARTAIADTVAGLRNA
                     PVSQDELTRARAPMIENMDNMLKSNAGWMTLVDRAQSEPEQIDRFLKTKELLNAITPA
                     QLQAAARRYLTEGGAVEVNVLPEGVPDPVAGPNSAQ"
     gene            2578..3078
                     /locus_tag="VFV30_00380"
     CDS             2578..3078
                     /locus_tag="VFV30_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846571.1"
                     /translation="MRKLIHAAILAGLSLAASSATADPRPSPRLPDLFLRFTVMTGSD
                     DLRGGRDNVMVTPIIRGNPTREWMLNQRAMRWADRSTHSTTIPLPPNTRPRDVEMVVL
                     RTTFRGGIDGDNWNMTSVTIEMTDASRSAYVKIGEGGPKRFTGDDRKLRIPVQLPGLS
                     DTLKTN"
     gene            complement(3179..5530)
                     /locus_tag="VFV30_00385"
     CDS             complement(3179..5530)
                     /locus_tag="VFV30_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4846572.1"
                     /translation="MTRLANRLTVSAAALAVALSASPALAAAEDLAAADAPAADAAAD
                     GSDTGLTEIVVTATKRETNLQRTPISISVMGAEEISKRQVQSLGDLGDGSVPSLRVAT
                     FESRQSALTVGIRGIVPDDANQPAREQGVGIYLDGVYLGRQHGLNASLYDVERIEVLK
                     GPQGTLFGLNTEGGAVSMVPKQPTGVFGGRISGGVGNYGSYNTQVHVNLPEVANVSIK
                     LDGVIQHQDATTRNPMPGQAGWNQYHRYGGRIAARWKPVDGLIVDLAFDKSRDENTPF
                     YSQLVTITPTALANIPRNTAGQPLIQYGFGRMDVADIQVPQEPSVGKAQGFTASVRYE
                     LTSDIELRSITAWRTVSDDQWDNSGGAHRTPVWGPNVNFSRYSLATLDQRQFSQELQA
                     VGSIGDSVDFVAGLYYFNERANDSARTPNTNRWNATLTGYTVNDPLTFQTAAVSRASI
                     AFSKSYAAYGQVTWAITEPLRVTVGGRYTRDEKNGRLFTVNGAATNFTFNQENNRFDP
                     LAIIAWQVNPDINVYAKYATGYRAGGASSRSLTYLSFGPEEVKSYELGFKSELFDRKV
                     RFNVAGYIMDRNNTQVDFNFFIPQTNGTVRNTLETVNAEGITKIRGIEADLTVRPVEG
                     LSMSLSYAYTYTKVPPARNTVQEALNASLIPPVTTPVFQTVYILYTPPHALSGAIDYD
                     MPIGSNGTMLRFHLDANFSDRMHSFANEPTLTDKSFLVNARLSLADIPMGGDNQTLTV
                     ALWARNLLNEEHIYRRSAANTTLGDYANYNAPRTYGVDVTLKF"
     gene            5771..6469
                     /locus_tag="VFV30_00390"
     CDS             5771..6469
                     /locus_tag="VFV30_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4846573.1"
                     /translation="MPTLTGTKTLLVTSPDPALARSVAAARPDLRFVPLDARIDPALP
                     IDGPAWCFVDWLLPDTSGLEVVRRLREARGTREAHITMVLDDHDSEERRRALKAGADD
                     YMTGPLSAAALLARMAQYETAGSAAGPARSRLVNGDLAVDLAAHQLRYRGTPIALRPN
                     EFRLLVHFMEHPDQVFSRQALIERLGKDEEAIDERTVDVWVGRLRRALAAHGAPDPLR
                     TVRALGYVMDSSAP"
     gene            complement(6458..>6879)
                     /locus_tag="VFV30_00395"
     CDS             complement(6458..>6879)
                     /locus_tag="VFV30_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX4846574.1"
                     /translation="SALSGKLPEPFRSESAGGGGVKMHHKFIVIDFDKPTARVYTGSF
                     NFSYAADQLNGENLLLFRDRRIATAYMVEAVRIFDSYRFRVKQAETKKKAAGDAAQPK
                     RMSLRKPPKTGEEAWWLRFWTDPNKVRDRELFALSGG"
BASE COUNT         1164 a         2271 c         2346 g         1098 t
ORIGIN      
        1 gcggacacca acgcctcgac cgggtttgag cggacgacct acatgctcga cctgcccaag
       61 aacgatcccg aactgctcga cgtcgcgctg atgctgatgc gcgagacggt gagcgagctg
      121 accttcagcc ccggcgcggt ggagcgcgag cgcggggtcg tccaggccga aatgcgcgac
      181 cgcaacacct ttgccctgcg caacgtcaag gaccagctgg actttgcctt cccaggatcg
      241 aaggtggcca accgcctgcc gatcggtacg ccggaatcgc tggccggggc gacgccggaa
      301 gggctgaagg ccttctggca gcgcgagtac gtgcccgaac acaccacgct ttacgtgatc
      361 ggcgatttcg atcccgacat ggtcgagcaa aagatcaagg accgctttgg cagctggaag
      421 ggcaaggccc cggccgagcc caagcccaag gccggcccga tcgcgatcga gacccagggc
      481 ggcgaggacg tctatgtcga tccggcgctg tccgaacgcg tctccgtctc gcgcaagggc
      541 gcctgggttg aagagcccga cagcctggcc cagcggcgcg agaacctgct gcggcagatc
      601 gcctatggca tcatcaaccg ccgcctgctg cgccgctcgc gcgaggagaa cgccccgttc
      661 cgcaacgcca gcttcggcac cggcaacatc ttcaaggaag gccgttcgac cgagctcaac
      721 atcgatacgg tcgacggcaa gtggcgccgc gggatgatcg aggcgggcct cgaataccgc
      781 aaggcgctga cctatggctt caccaaggaa gaggtcgacg agcagctggc gatcatccgc
      841 aacgcccagg ccaacgccgc cgagggcgag gcgacccgca gcaacgcgca gctgctgggc
      901 gcggccatgg cgctggtcaa cgacgagcgg attccggaca ccccgcgcaa ttcgctgaac
      961 cggctggacg cgttcattcc cgaaatcacc cccgatgccg tggtcggcgc gctgcgccgc
     1021 gacatggtgc cgctggagga accgcagatc cgcttccagg gccgcacgcc cccggccgat
     1081 ggcgaaaagg gcctgcgcga ggcctggaag caggcgatga ccgcgccgat ccgcccgaac
     1141 gagcgcaaag cggccggcaa ctttggttat accgacttcg gcccgccggg acgcgtggtc
     1201 agcgacgtgc gcgaaccgcg cttggggatc cgcacgatcc gctttgccaa cggggtgcgg
     1261 ctgaacatca agcgcaccga cctgaggaag gaccaggtgc tggtccaggt ttcggtcgac
     1321 ggcggggacc tcttggccac ggccgacaag ccgctggcga cgcggatgat gtcggtcctg
     1381 tcgctggggg gcctgggcaa gcacagcaag gacgagctgg acacgatgct ggccggcaag
     1441 accgtgtcgt ccaactattc cgccaccgac gaaacctttg tcgcgattgc ctccaccact
     1501 ccgcgcgatc tggaactgca gctgcaggtg ctgaccgcgc agatcgtcga tccgggctac
     1561 cgcaaggaag gcgaggtgct gttccggcag accatcaaca acctgttcgc cagcctgcgc
     1621 gccaccccgg ccgcggcgct ttcggccgat ctgggcgata tcctgtccgg cggcgatccg
     1681 cgcttcagcc tgggcaaggt ggaggattac cgcacgctga cctttgacca gctgcgcggc
     1741 acggtcagcg accggttcaa gaacggcgcg atcgagatcg gcatcgtcgg cgatgtggac
     1801 gaggacgcgg cgatcgcgct ggtggccaag acctttggcg ccctgcccca gcgcgaggcc
     1861 gaattccgcc cctatgccga ccgccgcgac cggcccttca ccaaggagcg caagcgccac
     1921 gtgctgcgcc acaccgggca gaaggaccag gcgatcgtca cttacatctg gccgacccgc
     1981 gacggcgagg atgccgcggc ggatgcggag ctggagctgc tcgaacgggt catgcggatt
     2041 gaaatgaccg acaccctccg cgaaaagctg ggcaaggcct attccccggg ggcgagcagc
     2101 aacacctcgc ggacctggcg caattacggc accttcagcg tcaacgcctc ggtcgcggtg
     2161 gccgaagtgc cggccgcccg cacggcgatt gccgatacgg tggcaggcct gcgcaacgcc
     2221 cccgtcagcc aggacgaact gacccgcgcc cgcgcgccga tgatcgagaa catggacaac
     2281 atgctgaaaa gcaacgcggg ctggatgacc ctggtcgatc gggcgcaaag cgagccggag
     2341 cagattgacc gctttctgaa gaccaaggag ctgctgaacg cgatcacccc ggcccagctg
     2401 caggccgccg cgcggcgcta tctgaccgag ggcggcgcgg tggaagtcaa cgtcctgccg
     2461 gaaggggtgc cggatccggt ggccgggccg aacagcgcgc aataggcccc ggattgaccc
     2521 ttgccgcagg gtcaggcatc ttcccgcgcg ttgcttatgc aagggagggg ccctgacatg
     2581 cgcaaactga tccacgccgc aatcctggcc gggctgagcc tggccgccag ttccgccacg
     2641 gccgatccgc ggccttcgcc gcgcctgccg gacctgttcc tgcgcttcac cgtaatgacc
     2701 ggcagcgacg atctgcgcgg cggccgcgac aacgtgatgg tcacgccgat catccgcggc
     2761 aacccgaccc gcgaatggat gctgaaccag cgggcgatgc gctgggccga ccgctcgacc
     2821 cattcgacca cgatcccgct gccgcccaac acccggccgc gcgatgtcga gatggtcgtg
     2881 ctgcgcacca ccttccgggg cggcatcgac ggtgacaact ggaacatgac cagcgtcacc
     2941 atcgagatga ccgacgccag ccgcagcgcc tacgtcaaga tcggcgaagg cggacccaag
     3001 cggttcaccg gggatgaccg caagctgcgc atcccggtgc agctgcccgg cctctccgac
     3061 acgctgaaga caaactaacg cgcccgcgcc gatcccggcc ccggccgtcc tgcacaaaaa
     3121 gcgggggcga agtgccaggc actccgcccc cgagcaggga ggaacggatc ggacgggatc
     3181 agaacttcag cgtgacatcc acgccgtacg tgcgcggggc gttgtagttc gcatagtcgc
     3241 ccaacgtggt gttggccgcg ctgcggcggt aaatgtgttc ttcgttgagc aggttgcggg
     3301 cccacagcgc gacggtcagc gtctggttgt cgccgcccat cgggatgtcc gccaggctga
     3361 gccgcgcgtt caccaggaag ctcttgtcgg tcagcgtcgg ctcgttggcg aagctgtgca
     3421 tccggtccga gaagttggca tcgaggtgga agcgcagcat cgtgccgttg ctgccgatcg
     3481 gcatgtcgta atcgatcgcg cccgacagcg cgtgcggcgg ggtgtagagg atgtagacag
     3541 tctggaacac cggcgtcgtc accggcggga tgagcgaggc gttcagcgct tcctgcaccg
     3601 tgttccgcgc gggcgggacc ttggtgtagg tgtaggcata ggacaggctc atcgacaggc
     3661 cttcaaccgg gcgcacggtc aggtcggcct cgatcccgcg gatcttggtg ataccttccg
     3721 cgttcaccgt ttccagcgtg ttgcggaccg tgccgttggt ctgcgggatg aagaagttga
     3781 agtccacctg ggtgttgttg cggtccatga tgtaaccggc cacgttgaag cggaccttgc
     3841 ggtcgaacag ttcggacttg aagcccagtt cgtaggactt cacttcctcc ggcccgaacg
     3901 acaggtaggt cagcgagcgc gagctggccc cgccggcgcg gtagccggtg gcgtacttgg
     3961 catagacgtt gatgtccgga ttgacctgcc aggcgatgat cgccagcgga tcgaagcggt
     4021 tgttttcctg gttgaaggtg aagttggtgg ccgcgccgtt gacggtgaac aggcgcccgt
     4081 tcttctcgtc gcgggtatag cggccgccca cggtgacccg cagcggttcg gtgatcgccc
     4141 aggtgacctg gccataggcg gcatagctct tgctgaacgc gatcgacgcg cgcgaaacgg
     4201 ccgcggtctg gaaggtcagc ggatcgttga cggtgtagcc ggtcagcgtg gcgttccagc
     4261 ggttggtgtt gggcgtgcgc gcggagtcgt tcgcccgctc gttgaagtaa tagagcccgg
     4321 cgacgaaatc gacgctgtcc ccgatcgagc cgaccgcctg cagttcctgg ctgaactggc
     4381 gctggtcgag cgtggccagc gaatagcggc tgaagttgac attcgggccc cacaccgggg
     4441 tgcggtgcgc accgcccgaa ttgtcccact gatcatcgct gaccgtgcgc caggcggtga
     4501 tcgagcgcag ctcgatatcg ctggtcagtt catagcggac actggcggta aagccctgcg
     4561 ccttgccgac gctgggctct tgcggcacct ggatatcggc cacgtccatc cggccaaagc
     4621 cgtactggat cagcggctga cccgcggtgt tgcgggggat gttggccagc gcggtcgggg
     4681 tgatggtgac cagctggcta tagaacgggg tgttctcgtc gcggctcttg tcaaaggcca
     4741 ggtcgacgat caggccgtcc accggcttcc agcgcgcggc gatgcggccg ccatagcggt
     4801 ggtactggtt ccacccagcc tgccccggca tcgggttgcg ggtggtggcg tcctggtgct
     4861 ggatcacgcc gtccagcttg atcgagacgt tggcgacttc cggcaggttg acgtggacct
     4921 gggtgttgta ggagccgtag ttgcccaccc cgccgctgat ccggccgccg aagacgccgg
     4981 tcggctgctt cggcaccatc gaaaccgcgc cgccttcggt gttgaggccg aacagggtgc
     5041 cctgcggccc cttcaggact tcgatccgct ccacgtcata gaggctggcg ttgaggccgt
     5101 gctggcggcc cagatacacg ccgtcgaggt agatcccgac gccctgttcg cgcgcgggct
     5161 ggttggcgtc atcgggcacg atcccgcgga tgccgaccgt cagggccgac tggcgcgatt
     5221 caaaggtggc gacgcgcagc gaagggaccg aaccgtcccc cagatcgccc aggctctgca
     5281 cctggcgctt ggagatttcc tcggcgccca tcaccgaaat cgagatcggg gtgcgctgaa
     5341 gattggtttc gcgcttggtg gcggtgacga cgatttcggt caggccggtg tcagagccat
     5401 cggcagcggc atcggcggcg ggagcatcgg cggcggcgag gtcctcggcg gcggccagcg
     5461 cgggcgatgc ggaaagcgcc acggcaagcg cggcggcgga aacggtaagc ctgttggcaa
     5521 ggcgggtcat ggtctggtcc ctcattgggt tgtctgtcgg cctgcgggga tgcggtgcga
     5581 ctcgccttgt gcagagtcga tcagccccgt tgccgaggcc ccgctaggcc cgcccgattg
     5641 cagcaaggcg actctttgcg gacaggcgaa tggcaggcgt gcgacagcgg tttgacagac
     5701 gcttttccgg gctccgccgt cacaaaaccg tcacgtgaaa gcggcattgt tgtcgcccga
     5761 gtggagcgcc atgccgacct tgaccggaac caagaccctg cttgtcacca gccccgatcc
     5821 ggcccttgcc cggagcgtgg cggccgcgcg tccggatctg cggttcgtgc cgctggacgc
     5881 ccggatcgat ccggccctgc caatcgacgg gccggcctgg tgctttgtcg actggctgct
     5941 gccggacaca tccggactgg aagtcgtccg ccgcctgcgc gaggcgcgcg gcacccgcga
     6001 agcccacatc accatggtgc tggacgatca cgacagcgaa gagcgccgcc gcgcgctgaa
     6061 ggccggggcc gacgattaca tgaccgggcc gctcagcgcc gccgcgctgc tggcccggat
     6121 ggcgcaatac gaaaccgccg gttcggccgc agggccggcc cgctcacgcc tggtcaacgg
     6181 cgatctggcg gtggacctgg ccgcccacca gctgcgctat cgcggcaccc cgatcgcgct
     6241 gcgccccaac gagttccgcc tgctggtcca cttcatggaa caccccgacc aggtgttcag
     6301 ccgccaggcc ctgatcgaac gtctgggcaa ggacgaagag gcgatcgacg agcggacggt
     6361 cgatgtctgg gtcggccggc tgcgccgcgc cctcgccgcc cacggcgcgc ccgatccgct
     6421 ccgcacggtc cgcgcgctgg gctatgtgat ggacagctca gccccctgac agcgcgaaca
     6481 gctcgcggtc gcggaccttg ttcggatcgg tccagaaccg cagccaccag gcctcctcgc
     6541 ccgtcttggg cggcttgcgc agcgacatgc gcttgggctg ggcggcatcg ccggcggcct
     6601 tcttcttggt ttccgcctgc ttcacccgaa agcgatagga atcgaagatc cgcacggctt
     6661 cgaccatata ggccgtggcg atccggcggt cgcggaacag cagcaggttc tcgccgttga
     6721 gctggtcggc cgcgtaggag aagttgaacg agccggtgta gacccgcgcg gtcggcttgt
     6781 cgaaatcgat cacgatgaac ttgtggtgca tcttcacgcc cccgcccccg gcgctttcgc
     6841 tgcggaacgg ttcgggcagc ttgccgctga gcgcgctgg
//
LOCUS       DASUDV010000018         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1436567,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000018 DASUDV010000000
VERSION     DASUDV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436567"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFV30_00400"
     CDS             <1..966
                     /locus_tag="VFV30_00400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4846575.1"
                     /translation="EVRAPNGQVLASWSYDHTMVRSWLEHQANALIWPHPSVARIERG
                     GALLAEVRLYGNSGGILSYGLSGLLISFACVGLTVIATRILARRLQRDVIDPLDHVAQ
                     VAHAVRRERAFERRVPSSGIAEIDRFGQDFNALLAELQGWHAGLTSENAELARKATHD
                     PLTGLGNRVLFEQTLGQAMGEAARVGASFAVLYLDIDNFKQINDAHGHESGDAALIAV
                     GERLRSALRQIDHAFRLGGDEFAALLLPLQDRTHVDGVVERIRQVMELPFRLPSGETV
                     VSNLSIGVAVYPDDGSSQQDLLRRADSEMYKQKKQAQLSGPSEGN"
     gene            967..1452
                     /locus_tag="VFV30_00405"
     CDS             967..1452
                     /locus_tag="VFV30_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4846576.1"
                     /translation="MRQILVLCLAVALAACQTAPKKLGFSKQQVETLSAEGFKPVEDN
                     YELGLLDRVLFAVDQSDLSPEATGVIDRLAKVLLGVGIHGAGIDGHTDSTGSTEYNKS
                     LSDRRAASVKRQMAASGMAEAELRARGLGETMPVAGNDTDDGRAQNRRVVIIVTPLDA
                     N"
     gene            1491..>3857
                     /locus_tag="VFV30_00410"
     CDS             1491..>3857
                     /locus_tag="VFV30_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX4846577.1"
                     /translation="MSTHWQFWIDRGGTFTDVVARAPDGRVLTAKLLSEDPNRPEDAA
                     VAAIRQLSGVGQGTLPPAEVRMGTTVATNALLERKGEPCVLAITRGFEDALQIGSQER
                     PELFVRQIVLDQPPCARVIGIDERIGADGSVLCPLNEDAARLAFAQAVADGLKAIAIV
                     LVHGWCYPAHEQRLAELAREAGFAQVSVSHQVSPTIKLIPRGDTTLVDGYLSPVLHRY
                     IDAFAAQLGGHIAPLFMQSSGGLVDGARFCGKDAILSGPAGGIVGMARTAENSGEARI
                     IGFDMGGTSTDVSHYAGAFERDGETRVAGTRITTPMLRIHTVAAGGGSICRFDAGRLV
                     VGPQSAGAVPGPACYRRGGPLTVTDCNVMLGKLIPEQFPQVFGPTGDQPIDRAAVADR
                     FAEVAAAIEAETGKAMAPQAIAEGFVQIAVANMANAIRKISVARGHDVTTHALVCFGG
                     AGGQHACLVADALAIDTVLIHPLAGVLSAYGMGLASEAVVREASLGLPLAPGVEARVE
                     ELRARLAGEACDQLGTQPSRIEARLFLRRHGSESLIGLDPAPVAELEAGFVAAHEAEF
                     GFAGSGALVIDRVRVEAVAERADAGNLAWPLPDVSDPPLVEVECWMDGQTCPVPVYQR
                     AGLAAGFNARGPLIVCDPLSTTVVEPGWAVGLDRDGTLRLTRYAPRLQANASAEEADP
                     VRLEIFNGLFMAIAEEMGSALARTASSVNIRERLDFSCAIFDEAGRLVANAPHMPVHL
                     GSMGESVARVIAARGNGSDGRGVRRGDAYVLNAPYAGGTHLPDITVIMP"
BASE COUNT          653 a         1185 c         1317 g          702 t
ORIGIN      
        1 gaggtgcggg cgccgaatgg acaggtgctg gccagctgga gctatgacca caccatggtg
       61 cgctcctggc ttgagcatca ggccaatgca ctgatctggc cccatccatc agtggctcgc
      121 atcgagcgcg gtggagcgct gctcgccgaa gtgcgcctgt acggaaattc cggtgggatc
      181 cttagctacg ggctgtccgg cctgctgatc tctttcgcct gcgtcgggct tacggtcatc
      241 gccacgcgaa ttctggcgcg gcgtctgcag cgcgatgtga tcgacccgct cgatcatgtt
      301 gcccaagtgg cgcatgcggt gcggcgcgaa cgcgctttcg agcgccgcgt cccttcgtcg
      361 gggatcgcgg aaatcgatcg cttcggccag gacttcaatg cgctgctggc cgagttgcag
      421 ggctggcatg ccggcctgac ttcggaaaat gccgagcttg cgcgcaaggc aacccacgac
      481 ccgctgaccg gccttggcaa cagggttctg ttcgaacaga ccctgggcca ggcaatgggc
      541 gaggcggccc gggtcggtgc ctcgtttgcg gtgctctatc tcgacatcga caacttcaag
      601 cagatcaacg atgcgcacgg gcacgaatcc ggggatgcgg cgctgatcgc ggtgggtgaa
      661 cgactgcgca gcgccttgcg ccagatcgac catgcctttc ggctgggcgg agacgaattc
      721 gccgcgctgc tactgccctt gcaggatcgc acccatgtcg atggggtggt cgaacggatc
      781 aggcaggtga tggaactgcc gttccgcctg cccagcggag aaacggtggt ttcaaatctc
      841 agtatcggcg tggctgttta tcccgacgat ggaagttccc agcaagacct gttgcgtcgg
      901 gccgattccg agatgtacaa gcagaagaag caagctcagc taagcgggcc gtcagaggga
      961 aattaaatgc gacaaatcct ggtactttgc ctggccgtgg cactcgccgc gtgccagacc
     1021 gcgccaaaga aacttggctt ttccaagcag caggtcgaga cgcttagtgc cgaaggcttc
     1081 aagccggttg aagacaatta cgaactgggc ctgctcgatc gcgtgctttt cgcagtcgat
     1141 caatccgatc tttcgcccga agcaaccggg gtgatcgatc ggctggcgaa ggtccttctg
     1201 ggcgttggca ttcatggcgc cgggatcgat ggccatacgg attcgaccgg cagcaccgag
     1261 tataacaaaa gcctgtccga tcggcgcgcg gcatcggtca agcggcagat ggcggcaagc
     1321 ggcatggccg aagcagaact gcgtgcccgt gggctgggtg aaaccatgcc ggtcgccggc
     1381 aatgacaccg acgatggtcg ggcgcagaac cgccgggtag tgatcatcgt taccccgctc
     1441 gacgccaact gaggtattga aggaaacgat ccagagccca taagttggca atgagcacgc
     1501 attggcaatt ctggatcgac cgcggtggaa cctttaccga tgtggtcgcc cgcgcgcccg
     1561 atggccgggt gctgacagcc aaactgctct ccgaggatcc taaccggccc gaagacgcag
     1621 ctgtcgccgc gatccgccag ctttcggggg tggggcaggg caccttgccg cctgccgagg
     1681 tgcggatggg caccacggtt gccaccaatg cgctgcttga acgcaagggc gaaccctgtg
     1741 tgctggcgat tacccgcggc ttcgaagacg cgctgcaaat cggcagccag gaacgcccag
     1801 aactgttcgt ccggcagatt gtgctcgatc agccgccctg cgcacgggtg atcgggattg
     1861 acgagcggat cggcgccgat ggatcggtgc tgtgtccgct gaacgaggat gcggcgcgcc
     1921 tcgccttcgc ccaggccgtt gccgatgggt tgaaggcgat cgcgatcgtg ctggttcatg
     1981 gttggtgtta ccctgctcac gaacaacgcc tggcggagct ggcgcgcgag gccggattcg
     2041 cgcaggtttc ggtgagccat caggtatcgc ccacgatcaa gctgatcccg cgcggcgata
     2101 ccacgctggt cgatgggtat ctctcccctg ttctgcaccg ctatatcgat gccttcgctg
     2161 cccagctggg cggacacatc gcgcccctgt tcatgcaatc gagcggcggg ctggttgatg
     2221 gcgcgcgctt ttgcggcaag gacgccatct tgtcgggccc ggccggtggg atcgtcggca
     2281 tggcacgaac tgcggagaat tctggcgaag cgcggatcat cggctttgac atgggcggca
     2341 cgtctaccga cgtcagccat tacgccggtg ccttcgaacg cgatggcgaa acccgtgttg
     2401 ccggcacccg gatcacaacg ccgatgctgc ggatccacac agttgcagcg ggcggcggtt
     2461 cgatctgccg cttcgatgcc ggccggctgg tggttggccc gcagagcgcg ggcgcggtgc
     2521 ccggtcccgc ctgctatcgc cgcggcgggc cgctgaccgt taccgattgc aacgtgatgc
     2581 tgggcaagct gatccccgaa cagttcccgc aagtgttcgg gcccacgggc gatcagccga
     2641 tcgaccgcgc cgccgtggcc gaccggtttg ccgaagtggc cgcggcaatc gaggcggaaa
     2701 ccggcaaggc aatggccccg caggccatcg ccgaagggtt cgtgcaaatc gcggttgcca
     2761 acatggccaa tgccatccgc aagatcagcg tggcacgcgg ccacgatgtc accacccatg
     2821 cgttggtctg ctttggcgga gcgggcgggc agcacgcctg cctagtggcc gatgccctgg
     2881 cgatcgacac ggtcctgatc catccgctgg ccggcgtgct atcggcctat ggcatgggtc
     2941 tggcgagcga ggcggtggtg cgcgaggcca gcttgggtct gccgctggca ccgggcgtcg
     3001 aagcacgggt cgaagagctg cgggcccggc tggccggcga agcctgcgac cagcttggaa
     3061 cccagcccag ccggatcgag gcacgcctgt tcctgcgccg ccacgggagc gaaagcctga
     3121 tcgggcttga tccggcgcct gtcgccgagc ttgaggcagg gtttgttgcc gcgcacgagg
     3181 ccgaattcgg ctttgctgga agtggcgccc tggtgatcga ccgggtgcgg gtggaagcgg
     3241 tggccgaacg ggctgatgcc ggtaatctgg cctggccctt gccggatgtc tctgacccgc
     3301 cgctggtcga agtcgaatgc tggatggacg ggcagacttg cccggttccg gtctatcagc
     3361 gcgcagggct ggctgccggc ttcaacgccc gggggccgct gatcgtgtgc gaccccctgt
     3421 cgaccacggt ggttgagccc ggctgggcgg ttgggctcga tcgcgatggc acgctcaggc
     3481 tcacccgcta tgctcctcgc cttcaggcaa acgccagcgc agaagaagcc gatccggtgc
     3541 ggctggaaat cttcaacggg ctgttcatgg cgattgccga ggaaatgggt tcggcgctgg
     3601 cgcgcaccgc cagttcggtc aatatccgcg agcggctcga tttctcctgc gcgatcttcg
     3661 atgaagccgg gcggctggtg gccaatgccc cgcatatgcc ggttcacctg ggctcgatgg
     3721 gcgaaagcgt ggcccgggtg atcgcggcac gcggtaacgg gtcggatggc cgcggggtgc
     3781 ggcgcggcga tgcctatgtg ctcaacgcgc cctatgccgg gggcacgcat cttcctgata
     3841 tcacggtcat catgccg
//
LOCUS       DASUDV010000019         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_398198,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000019 DASUDV010000000
VERSION     DASUDV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398198"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFV30_00415"
     CDS             <1..352
                     /locus_tag="VFV30_00415"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664036.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX4846578.1"
                     /translation="AYFECLHETKLIVDLLYEGGIANMRYSISNTAEYGDITTGPRII
                     TAETKAEMKRVLADIQSGRFVKNFVLDNRAGQPELKAARKAAAAHPIEQTGARLRAMM
                     PWIGANKLVDQDKN"
     gene            503..1870
                     /locus_tag="VFV30_00420"
     CDS             503..1870
                     /locus_tag="VFV30_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX4846579.1"
                     /translation="MSNSRLFGDSLNKREALRLISYGSLLLCDLASIQAGFAIASHWR
                     GAYWLQLYGYPVSVLFCIIYSLLALSSGSVTRDAFGSRLRSAMLGERTMLMASALCIA
                     ILFFQPHGLTLSRLSLLTAIVLSALFIGVGRILFLTLFLQNRDEFWTSCVMLVDGAPP
                     PPGTSATVFDVGAEGIDPERSDPAIVARLGTLLADYDKVVVICADSERANAWSLMLKA
                     FDIQAEVVMPEGVPIGAVGVGRMGDRPTLIVNRGQLSLASRIKKRLVDILFSAIALLL
                     LAPLLVVVAIAIKLDSPGPVLFRQSRVGYGNRPFRIYKFRSLRHEATDSNGNKSVSVG
                     DNRVTRVGAFIRKTSIDELPQLFNVLLGDMSLVGPRPHALGSRAGEKLFWEIDPNYWQ
                     RHALKPGITGLAQIRGFRGETQAQEDLTQRLNSDIEYLEGWTLWRDFRIMIGTVRVLM
                     HHKAF"
     gene            1900..>2786
                     /locus_tag="VFV30_00425"
     CDS             1900..>2786
                     /locus_tag="VFV30_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX4846580.1"
                     /translation="MSNVRLIIGFAMAIALLGVLALAWQQGLFGDPARTPGGEAARQR
                     ELAVLPGKPGAEPVAQPSLDPLTALNQNAAVPFVKGPITPARAFKFTGSAADRLQATE
                     CLALTAMAEAGGSDPGQRAVIQVVLNRVRHPAFPKTICGVVFQGSERATGCQFTYTCD
                     GSLARRYPDLAWQAARKRAREALDGYVYKPVGLATHYHTNWVYPYWSPSLDKVAQVDT
                     HLFFRWRGFWGTPAAVQSRYGGGEPSFAALVQQVQVSDPLASEAAVDAASEAADVAKI
                     GSLAAGKAAGELVIPHPDGG"
BASE COUNT          465 a          910 c          889 g          522 t
ORIGIN      
        1 ggcctatttc gagtgcctcc acgaaaccaa gctgatcgtc gacctgctct atgaaggcgg
       61 catcgccaac atgcgctatt cgatcagcaa caccgccgaa tacggcgaca tcaccaccgg
      121 cccgcggatc attaccgccg aaaccaaggc ggaaatgaag cgcgtgctgg ccgacatcca
      181 gtcgggccgc ttcgtgaaga acttcgtgct cgataaccgc gccggccagc cggaactgaa
      241 ggccgcgcgc aaggctgccg ccgcgcaccc gatcgagcag accggtgccc gcctgcgcgc
      301 catgatgccc tggatcggtg ccaataagct ggtcgatcag gacaagaact gatcgtttac
      361 ccgcatctgc taacaacggc gatgcccgct gcgtgatagt gcgcggcggg cattgccagt
      421 tttgagcccg ggagcctata tcaacggctc ctgcagcatg gtgagggaac aggaacttgc
      481 attcgcccgc gagcaggccg cgatgagcaa cagccgtctg tttggtgact cgctgaacaa
      541 gcgcgaggcg ctccggctga tcagctatgg gtcgctgctg ctgtgcgatc tggcctcgat
      601 ccaggccggt ttcgcgatcg cttcgcactg gcgcggggcc tattggctcc agctctacgg
      661 ttatcccgtc tcggtgctgt tctgcatcat ctattccctg ctcgcgctgt cgagtggcag
      721 cgtgacccgc gatgccttcg gatcccgact gcgttcggcc atgttgggtg aacggaccat
      781 gctgatggcc agcgcgctgt gcatcgccat cctgtttttc cagccgcacg gcctgacgct
      841 gtcgcggctg tcgctgttga ccgccatagt tctgtcggcg ctgttcatcg gtgtaggccg
      901 gatcctgttc ctgacgctgt tcctgcagaa ccgcgatgaa ttctggacca gctgcgtcat
      961 gctggtcgat ggcgcgccgc cgccgccggg caccagtgcg accgtgtttg acgtgggcgc
     1021 cgaagggatc gacccggaac gaagcgatcc cgcaatcgtc gcccgactcg ggaccttgct
     1081 ggcggactac gacaaggtcg ttgtgatctg cgcggacagc gaacgggcca atgcctggag
     1141 tctgatgcta aaggcgttcg atatccaggc cgaagtggta atgccggaag gggtgccgat
     1201 tggcgcggtt ggcgtcggcc gcatgggcga tcgtccgacg ctgatcgtta accgcggcca
     1261 gctctcgctt gccagccgga tcaagaagcg gctagtcgat attcttttct cggcaattgc
     1321 cctcttgcta ctggcgccgc tgctggtggt ggtggcgatt gcgatcaagc tcgacagccc
     1381 ggggcccgtc ctgttccgcc agtcgcgggt cggctatggc aaccgcccgt tccggatcta
     1441 caagttccgc tcgctgcgcc acgaggcgac cgacagcaat ggcaacaaat cagtctcggt
     1501 cggagacaac cgggtaaccc gcgtcggagc cttcatccgc aagacctcga tcgacgagct
     1561 gccgcagctg ttcaacgtcc tgctgggtga catgagcctg gttggccccc gcccccatgc
     1621 gctaggctca cgcgccggcg aaaagctgtt ctgggaaatc gatcccaatt actggcaacg
     1681 ccatgcgctg aagcccggga tcaccggcct ggctcagatc cgcggtttcc ggggtgagac
     1741 ccaggcgcag gaagacctga cccagcggct caattccgac atcgagtacc tggaaggttg
     1801 gaccctgtgg cgcgatttcc gcatcatgat cggcacagtg cgggtgctga tgcaccacaa
     1861 ggcgttctga ccatgcccgc cgctgcggcc cgcgtgccga tgtccaatgt gcgtctgatc
     1921 atcggttttg ccatggctat cgcgctgctt ggcgtgctgg ccctggcctg gcagcagggc
     1981 ctgtttggcg atcccgcccg caccccgggt ggtgaggcgg cgcgacagcg tgagctggcg
     2041 gtcttgccgg gcaagccggg ggccgaaccg gtggcgcagc cgtcgctcga tccgttgacc
     2101 gcgcttaacc agaatgccgc cgtgccattt gtcaaaggcc cgatcacccc cgcccgcgct
     2161 ttcaagttca cgggctcggc ggctgaccgg ctccaggcca ccgaatgtct cgcgcttacc
     2221 gccatggccg aagctggcgg cagtgatccc ggccagcgtg cggtaatcca ggtcgtactc
     2281 aaccgggtgc gccatccggc cttcccgaag acgatctgcg gcgtggtgtt ccagggatcc
     2341 gagcgcgcaa ccggctgcca gttcacctac acctgcgacg gctcgctcgc ccggcgctat
     2401 ccggacttgg cctggcaagc tgcccgcaag cgcgcgcgcg aggcgctgga tggctacgtc
     2461 tacaagccgg ttggcctggc cacgcactat cacaccaact gggtctaccc ctactggagt
     2521 cccagcctcg acaaggttgc ccaggtcgat acgcacctgt tcttccgctg gcgcgggttc
     2581 tggggaacgc cggcagcggt ccaatcgcgc tatggcggcg gcgagcccag ctttgccgca
     2641 cttgttcagc aagtccaggt cagcgatccg ctggccagcg aagccgccgt ggatgcggcg
     2701 agcgaggcgg ctgatgtggc caagatcggc agtctggctg cgggcaaggc tgcgggagag
     2761 ctggtcattc cccaccccga cggcgg
//
LOCUS       DASUDV010000020         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1016805,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000020 DASUDV010000000
VERSION     DASUDV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016805"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VFV30_00430"
     CDS             <1..1887
                     /locus_tag="VFV30_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4846581.1"
                     /translation="ESYAPFAAPASRLIPAIRSAEARRPPLRERAGKNNIDIPSIQAN
                     TVARVGGQAYLLTATLVQPDFGKVLPKGPQAPITITALPLDAAFLKSFGERYLLDGLR
                     LADQILPASGERTVQLRGANGRAVASLAWTPRQPGTMILEDFRVEALLALLGLFLVGL
                     TVVRRGAAIVTELIASEGQARHLAYHDALTKLPNRALLFDRLPALLADPARNGRALAV
                     ICVDLDRFKDVNDTLGHHAGDLLIAAVAGRLGEVAEDTALIARLGGDEFVLLCEDVDR
                     ERAETLSRRILAAVLRPLETEHGRIEVGCSIGIALIDQPGLEPSEVLRWADLALYRSK
                     ERGRGQFQFFEPDMDAELRDRRALEMDLRRALASDGLKLVYHPQVDRQGRVRAVEGLL
                     RWQDPRRGAIPPSRFVPLAEECGLILPLGEYVLRRIFAETAAWQCVRIAVNISAVQLR
                     APGFAAQVMRLAAQAGIDPARYEIELTETALLGDEPATAANVEALKRMGFSIALDDFG
                     TGYSSLSVLQRFSVDRIKIDRSFVSDLGGTAEAEALVDAMIKLAKALGVSVVAEGVEN
                     AVQFNRLILCGCREFQGHLFGLPQGRAEIEAAYGLASPAADAAEDPAEETGTAEADAV
                     KRRA"
     gene            complement(1871..2200)
                     /gene="grxD"
                     /locus_tag="VFV30_00435"
     CDS             complement(1871..2200)
                     /gene="grxD"
                     /locus_tag="VFV30_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX4846582.1"
                     /translation="MSDTNARIAEIVSGNDVVLFMKGTPLFPQCGFSSRAVAILEHLG
                     VTYEGVDVLQDMEIRQGIKAFSDWPTIPQLYVKGEFVGGSDIMMEMYEAGELQQMLAD
                     AGLTPAA"
     gene            complement(2223..2450)
                     /locus_tag="VFV30_00440"
     CDS             complement(2223..2450)
                     /locus_tag="VFV30_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX4846583.1"
                     /translation="MPGAEIIARIKAGIPDAEVTLTDLAGDDNHWAAHVVSAAFIGKP
                     RVAQHKLVYAAIGEDMGGALHALQLTTAVPK"
     gene            complement(2461..2784)
                     /locus_tag="VFV30_00445"
     CDS             complement(2461..2784)
                     /locus_tag="VFV30_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX4846584.1"
                     /translation="MTDFNDRERAEEAKFAHDEEMQFRIQARRNRLLGGWAAEKMGLS
                     AVEAEAYAKAVVQTEFEEAGDEDVIRKLLGDLVSAGLDVSESDVRAAMDAKAVEARRQ
                     LMGEV"
     gene            complement(2810..3301)
                     /locus_tag="VFV30_00450"
     CDS             complement(2810..3301)
                     /locus_tag="VFV30_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4846585.1"
                     /translation="MIAARIVIAGALLATGWAVYAKPAPAPKPTAPSAQDLVQARQAG
                     MVMAATTMNLLRGASNNGTPLKNLAFSAGGLAKWAAAAPALFADSTRGVPSRALPAVW
                     TDKAGFAAKSKAFADSTRALADAAKAEDKAAFDAALASTAASCKGCHDSYQAPPGPAP
                     KAG"
     gene            complement(3331..>3987)
                     /locus_tag="VFV30_00455"
     CDS             complement(3331..>3987)
                     /locus_tag="VFV30_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4846586.1"
                     /translation="ETGASLLMTYGTNMHGLLDRGRLKAGETLLVLGAAGGIGLSAVE
                     LGKAYGARVVAAVSSEEKAEAAREAGADEVVIYPRAPFDKDQSKALAEMFKAACGPNG
                     ADVIYDVVGGDYSEPALRSIAWEGRFCVVGFPAGIAKLPLNLTLLKSCDVCGVFWGAW
                     VAREPAANAAHIAKLFDLLKDGKIKPKISATFPLARGGEAITMLAERKAIGKVVVTMD
                     "
BASE COUNT          580 a         1349 c         1412 g          646 t
ORIGIN      
        1 gagagctatg cccccttcgc ggccccggcc tcgcggctga tcccggcgat ccgcagcgcc
       61 gaggcccgcc gcccgccgct gcgcgaacgg gccggcaaga acaacatcga catcccctcg
      121 atccaggcca acacggtcgc ccgggttggc gggcaggcct atctgctgac cgcgaccctg
      181 gtccagccgg acttcggcaa ggtcctgccc aaggggccgc aagcgccgat cacgatcacc
      241 gcgctgccgc tcgacgcggc cttcctgaaa tcctttggcg aacgctacct gctcgacggg
      301 ctgcggctgg cggaccagat cctgccggct tcgggcgagc ggaccgtgca gctgcgcggg
      361 gcgaacgggc gggcggtagc ctcgctggcc tggaccccgc ggcaaccggg aacgatgatc
      421 ctggaggact tccgggtaga ggccctgctg gccctgctcg ggctgttcct ggtcggcttg
      481 acggtggtac ggcgcggcgc ggcgatcgtg accgagctga tcgccagcga aggccaggcc
      541 cggcacctgg cctatcacga tgccctgacc aagctgccca accgcgccct gctgtttgac
      601 cggctgccgg cgctgttggc cgatccggcc cgaaacgggc gggccctggc ggtgatctgc
      661 gtcgatctgg accggttcaa ggatgtcaac gacacgctgg gccatcacgc cggcgatctg
      721 ctgatcgcgg ccgtggccgg gcggctgggc gaagtggccg aggacaccgc cctgatcgcg
      781 cgcctgggcg gcgatgaatt cgtgctgctg tgcgaggatg tcgaccgcga gcgcgccgaa
      841 accctgtccc gccggatcct ggcggcggtg ctgcggccgc tggaaaccga acacggccgg
      901 atcgaggtgg gctgctcgat cggcatcgcg ctgatcgacc agccggggct ggagccgtcc
      961 gaagtgctgc gctgggccga tctggcgctt taccggtcca aggagcgcgg ccgcggccag
     1021 ttccagttct tcgaacccga tatggacgcc gagctgcgcg accggcgggc cctggaaatg
     1081 gacctgcggc gggcgctggc cagcgacggc ctgaagctgg tctatcaccc gcaggtggac
     1141 cggcaggggc gggtccgggc ggtggaaggc ctgctgcgct ggcaggatcc ccggcgcggc
     1201 gcgatcccgc cgtcgcggtt cgtgccgctg gcggaggagt gcggcctgat cctgccgctg
     1261 ggcgaatacg tgctgcgccg gatctttgcc gagaccgcgg cctggcagtg cgtgcggatt
     1321 gcggtcaaca tctccgcggt ccagctgcgc gcgccgggct ttgccgccca ggtgatgcgc
     1381 ctggcggccc aggccgggat cgatcccgcg cgctatgaaa tcgaactgac cgaaaccgcc
     1441 ctgctgggcg acgaaccggc caccgccgcc aatgtcgagg cgctgaaacg gatgggcttc
     1501 tcaatcgccc tcgatgattt tggcaccggc tattccagcc ttagcgtgct gcagcgcttc
     1561 tcggtcgacc ggatcaagat cgaccggtcg ttcgtcagcg acctgggcgg cacggccgag
     1621 gccgaggcgc tggtcgatgc gatgatcaag ctggccaagg cgctgggcgt cagcgtggtg
     1681 gccgaagggg tggagaacgc ggtccagttc aaccgcctga tcctgtgcgg ctgccgcgaa
     1741 tttcagggcc acctgttcgg cctgccgcag ggccgggccg agatcgaggc ggcttacggt
     1801 ctggccagcc cggccgcgga tgcggccgag gacccggcgg aggaaacggg gacggccgaa
     1861 gccgacgcgg tcaagcggcg ggcgtaagcc cggcgtcggc cagcatctgc tgcagttcgc
     1921 cggcctcgta catttccatc atgatgtcgc tgccgccgac gaattcaccc ttcacgtaga
     1981 gctgggggat ggtcggccag tccgaaaagg ccttgatgcc ctgccggatt tccatgtcct
     2041 gcagcacgtc gaccccttca taggtcaccc caaggtgttc gaggatcgcg acggcgcggc
     2101 tggaaaagcc gcactgcggg aacagcggcg tgcccttcat gaacagcacg acgtcgttgc
     2161 cggagacgat ttcggcgatg cgggcattgg tatcggacat ggtgatttgg ctcctcgccg
     2221 ccctacttgg gaacggcggt ggtcagttgc aaggcgtgga gggcaccgcc catgtcctcg
     2281 ccgatcgcgg catagaccag cttgtgctgg gcaacgcgcg gcttgccgat gaaggcggcg
     2341 gagacgacgt gggcggccca gtggttgtca tcgccggcca ggtcggtcag cgtcacttcg
     2401 gcatcgggga taccggcctt gatccgcgcg atgatctcgg ccccgggcat cggcacggga
     2461 tcagacctcg cccatcagct ggcggcgggc ttcgacggcc ttggcatcca ttgccgcgcg
     2521 gacatcggat tcgctgacat caagcccggc cgaaaccaga tcgcccagca gcttgcggat
     2581 cacgtcctcg tcgccggctt cctcgaactc ggtctggacg acggccttgg cataggcttc
     2641 ggcctcgacc gccgagagcc ccatcttttc ggccgcccag ccgccgagca ggcggttgcg
     2701 gcgggcctgg atccggaact gcatctcttc gtcgtgggcg aacttggctt cttcggcgcg
     2761 ctcgcgatcg ttgaaatcgg tcatgtctgc tcctgctggg ttgccgcgcc tagccggcct
     2821 tgggggccgg cccgggcggc gcctgatacg aatcgtggca gcccttgcac gatgccgcgg
     2881 tgctggcaag ggcggcgtcg aaggcggcct tgtcttcggc cttggcggcg tccgcaaggg
     2941 cgcgggtcga gtcggcaaag gccttggact tggcggcaaa accggccttg tcggtccaca
     3001 ccgccggaag cgcgcggctg ggcacgccgc gggtgctgtc ggcaaacagg gccggggcgg
     3061 cggcggccca cttggccagg ccgccggccg agaaggccag gttcttcagc ggcgtaccgt
     3121 tgttgctggc gccgcgcagc aggttcatcg tcgtggccgc catgaccatc ccggcctggc
     3181 gggcctggac cagatcctgg gcggacgggg ccgtgggctt gggggccggg gcgggcttgg
     3241 cgtaaacggc ccagccggtg gcgagcagcg cccccgcgat gacaatccgt gctgcgatca
     3301 tggctgttct cccgtgcggc gcagcgggcc tcagtccatg gtcaccacga ccttgccgat
     3361 ggccttgcgc tccgccagca tggtaatcgc ttcgccgccg cgtgccagcg ggaaagtggc
     3421 cgagatcttc ggcttgatct ttccatcctt cagcaggtcg aacagcttcg cgatatgggc
     3481 cgcgttggcg gccggctcgc gcgccaccca ggcgccccag aacacgccgc aaacgtcgca
     3541 gcttttcagc agggtcaggt tgagcggcag cttggcgatc ccggccggga agccgaccac
     3601 gcagaaccgg ccttcccacg cgatcgagcg tagcgccggt tcggaatagt cgccgccaac
     3661 cacgtcatag atcacgtccg cgccgttcgg gccgcaggcg gccttgaaca tttcggccag
     3721 ggccttggac tggtccttgt cgaacggggc gcggggatag atcaccacct cgtcggcacc
     3781 ggcctcgcgc gcggcctcgg ccttttcctc gctggaaacg gcggcgacga cgcgggcgcc
     3841 ataggccttg cccagttcca ccgccgacag gccgatcccg ccggccgcgc ccagcaccag
     3901 cagggtttcg cccgccttca gccgcccgcg atcgagcagg ccgtgcatgt tggtgccata
     3961 ggtcatcagc agcgaggcgc cggtttc
//
LOCUS       DASUDV010000021         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1480717,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000021 DASUDV010000000
VERSION     DASUDV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480717"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /gene="pgi"
                     /locus_tag="VFV30_00460"
     CDS             <1..1413
                     /gene="pgi"
                     /locus_tag="VFV30_00460"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4846587.1"
                     /translation="TRLDLPGGAIRFDWSKTHLDADHGAAFEALAEATGFKAKRGAMF
                     AGEVINVTEGRAVEHTAQRGVGRDTSCEEAFALHLRMKSLVEAIHAGALGEVKHLIHI
                     GIGGSALGPALALDALTRDGAKVDVHVVSNIDGCALEAAFKACDPATTLIAVASKTFT
                     TTETMTNAASALAWLGEGGVDDPYGRVVALTASPDKAVEWGVDETRVLPFKESVGGRY
                     SLWSSIGFPVALALGWDDFAEFLDGAAAMDRHFLEADGLCNLPLRAAFADLYYARLLG
                     CQTRACFAYDERLRLLPSYLQQLEMESNGKRVKADGTPVDGATAPITWGGVGTDAQHA
                     VFQLLHQGTHVVPVDFIAAIIPGDSLDPAHHRNLLANCFAQGAALMAGKDAKDGARAY
                     PGDRPSATILCEDLNPATLGALIAFHEHRTFANAVLMEINAFDQFGVELGKEIAKSIL
                     GGGVQFDASTEALLAEAGIK"
     gene            1480..>2066
                     /locus_tag="VFV30_00465"
     CDS             1480..>2066
                     /locus_tag="VFV30_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4846588.1"
                     /translation="MTDFDYDLFVIGAGSGGVRASRIAASHSARVAVAEEFRIGGTCV
                     IRGCVPKKLLVYASHFAHSLHDAPTYGWTLGETSFDWARLRDFVASDVDRLERAYTST
                     LDSNKVEHFHERATVAGPNTVRLASGREITAKTILIAVGAWPVMPEIDGAEHCITSNE
                     VFHLPELPKRIVIQGAGYIALEFAGVFNALGSQVTV"
BASE COUNT          331 a          684 c          725 g          326 t
ORIGIN      
        1 acccggctgg acctgcccgg cggggcgatc cggttcgact ggtcgaagac ccacctcgac
       61 gccgaccacg gcgcggcctt tgaggcgctg gccgaagcga ccgggttcaa ggcaaagcgc
      121 ggggcgatgt tcgcgggcga ggtcatcaac gtgaccgagg gccgcgcggt ggagcacacc
      181 gcccagcgcg gggtggggcg cgacaccagc tgcgaggaag ccttcgcgct gcacctgcgg
      241 atgaaaagcc tggtcgaggc gatccacgcc ggcgcgctgg gcgaggtgaa gcacctgatc
      301 cacatcggca tcggcggctc ggcgctgggc ccggcgctgg cgctcgacgc gctgactcgg
      361 gacggggcga aggtcgacgt ccatgtcgtc tccaacatcg atggctgcgc gctggaagcg
      421 gccttcaagg cctgcgatcc cgccaccacg ctgatcgcgg tcgcatcgaa gaccttcacc
      481 accaccgaaa ccatgaccaa cgccgcgtcg gccctggcct ggctgggcga gggcggggtg
      541 gacgatccct atggccgggt cgtcgcgctg accgccagcc cggacaaggc ggtggaatgg
      601 ggcgtcgatg aaacccgggt gctgccgttc aaggaatcgg tcggcgggcg ctattcgctg
      661 tggtcgtcga tcggctttcc ggtggccctg gcgctgggct gggacgattt cgccgaattc
      721 ctcgacggtg cggcggcgat ggaccggcat ttcctggagg ccgatggcct ttgcaacctg
      781 ccgctgcgcg cggcctttgc cgatctctat tacgctcgcc tgctgggctg ccagacccgg
      841 gcctgctttg cctatgacga gcggctgcgg ctgctcccca gctatcttca gcagctggag
      901 atggagagca acggcaagcg tgtgaaggct gatggcacgc cggtggacgg ggccaccgcg
      961 ccgatcacct ggggcggggt ggggaccgac gcgcagcacg cggtgttcca gctgctccac
     1021 cagggtaccc atgtggtccc ggtcgatttc atcgccgcga tcatcccggg ggacagcctc
     1081 gatccggcgc accaccgcaa tctcctcgcc aactgctttg cccaaggcgc cgcgctgatg
     1141 gcgggcaagg atgcgaagga cggcgcccgc gcctatccgg gcgaccggcc gagcgcgacg
     1201 atcctgtgcg aggacctgaa ccccgcgacg ctgggcgcgc tgatcgcgtt ccacgaacac
     1261 cgcacctttg cgaatgccgt gctgatggag atcaacgcct tcgaccagtt cggggtggag
     1321 ctgggcaagg agatcgccaa gtcgatcctg ggcggcgggg tgcagttcga cgccagcacc
     1381 gaagcgctgc tggccgaggc ggggatcaag tagcgggcct atcgccccgg cccacaaaac
     1441 cctgtggcaa ttccggcctg tccgccccat atcccgggaa tgacagactt cgactatgac
     1501 ctgttcgtga tcggcgccgg cagcggcggc gtgcgggcca gccggattgc cgccagccac
     1561 agcgcgcgcg tggccgtggc cgaggaattc cggatcggcg ggacctgcgt gatccgcggc
     1621 tgcgtgccca agaagctgct ggtctatgcc agccactttg cccactcgct ccacgacgcg
     1681 ccgacttatg gctggaccct gggcgagaca tcgttcgact gggcgcgcct gcgcgatttc
     1741 gttgccagcg atgtcgaccg gctggagcgg gcctatacct ccacgctgga cagcaacaag
     1801 gtcgagcatt tccacgaacg tgccactgtc gccgggccga acacggtgcg gctggcgagc
     1861 gggcgtgaga ttaccgccaa gaccatcctg atcgcggtcg gggcctggcc ggtcatgccc
     1921 gaaatcgacg gggcggagca ctgcatcacc tccaacgagg tgttccatct gcccgaactg
     1981 ccgaagcgga tcgtgatcca gggcgcgggc tatatcgcgc tggagttcgc cggcgtgttc
     2041 aacgcgctgg gcagccaggt gaccgt
//
LOCUS       DASUDV010000022         4778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_773818,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000022 DASUDV010000000
VERSION     DASUDV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773818"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1524)
                     /locus_tag="VFV30_00470"
     CDS             complement(13..1524)
                     /locus_tag="VFV30_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEX4846589.1"
                     /translation="MSGRLILILGDQLSSTLASLEAGDRARDRVLMAEVMAEASYVPH
                     HKRKIAFVFAAMRHFAAELRAAGWQVDYVTLDDPANTGTLAGEVQRARTRHALTGVVV
                     TEPGEWRLLEAMRGWPETELLADDRFLADRHGFADWAEGRKGLRMEHFYRLMRRKTGL
                     LMEGDQPAEGRWNFDAENRKPAAADLLMPRPPRFEPDAITRDVLDLVAARFPDNFGSL
                     DGFAMAVTRADAEAAFAHFLKHGLPQFGAYQDAMLAGQPFLYHAFISPQLNAGLLDPL
                     ALCRAVEAEWRAGRVPINSAEGFIRQIIGWREFVRGIYWWRMPGYDRVNALEARRPLP
                     SFYWSANSPMACLRACVEQTRDHAYAHHIQRLMVTGNFALLAGIDPHELHEWYLAVYA
                     DAYEWVELPNTVGMSQFADGGRLASKPYAASGAYIDRMSDYCRTCAYDVKAKAGPKAC
                     PFNLLYWDFIARHRERLARNPRMAQMVRTWDRFAPEKQEQLGRDAARFLDSLA"
     gene            1647..2090
                     /locus_tag="VFV30_00475"
     CDS             1647..2090
                     /locus_tag="VFV30_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4846590.1"
                     /translation="MLSQKTRYTIRALQHLADTVGQGPIRLDTIAEAQNIPRKFLTVI
                     LSEMVRAGLVISHRGRDGGYELGLAPVDIRYGDIIRLTRGSLALVPCASRNAYEHCQN
                     CLPEADCRLRALMLTLRDEMAAMLDRVTLADPIKTEPPFEDAEAG"
     gene            2127..3791
                     /gene="recN"
                     /locus_tag="VFV30_00480"
     CDS             2127..3791
                     /gene="recN"
                     /locus_tag="VFV30_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX4846591.1"
                     /translation="MLTRLAIRNVVLIEALDLDFAGGLGVLTGETGAGKSILLDALGL
                     ALGARADSGLVRAGEDQASVTASFEFDKLPMPIRAILAEAEVDIEPGEPLLIKRRVKA
                     DGGSKAFVNDQPVGAALLRELSGHLVELHGQHDDRGLVNPRGHRMLLDRFARADVAGV
                     EAAWQAWRAAQDALDAARAGIVKAAEDRDLLLAHLSELTALAPEVGEEEQLAEARATM
                     QKGEKLTDDLEALRKLWAGSDSALASLRSAARRLDRIAEEHPLLAEALGALDRAVIEA
                     SEAEEKLERAAEALAHDPAALDRIETRLFELRAAARKHQCQVDDLPHRMREMRAALDG
                     IEGGEAELAALEAAATAAAADYRARAEALSALRQEAAARLDSAVAGELAPLKLDAARF
                     RTAVTPQGEDRWGPAGLDAVEFLISTNPGADFAPLAKIASGGELSRFILALKVALAEE
                     GGAATVIFDEIDRGVGGAVASAIGERLARLAAGGQLLAVTHSPQVAARGQQHYVIAKS
                     SEGTVTRTSVHLLDAAGRQEEIARMLSGAEVTPEARAQADRLLEGV"
     gene            3791..4558
                     /locus_tag="VFV30_00485"
     CDS             3791..4558
                     /locus_tag="VFV30_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX4846592.1"
                     /translation="MKLKDLLLRAVKPSIHSKFDQAILQIPEFDDESEQPEALLDHAE
                     LETLSGLALIIEYENSKKEKTQRIVTCKQLAVQGEAEYLKAYCHHRKAVRTFRVDRIV
                     DVFDPKSGESLSPVQAFFAQFSPDKVTRSGLSWGLSVRKRADLIAMLNALVFIARCDR
                     DFHPTERLVLETALSSFWLRMEVAGDPDFDDILGYATRLSPDGETFWLAMQRFSEDQT
                     LSGIFRQQVRELIEADGVIREEEAYWALEIDEFLSCR"
BASE COUNT          802 a         1554 c         1628 g          794 t
ORIGIN      
        1 gaccgttgcc gctcaggcaa gggagtccag aaagcgggcg gcgtcgcgcc ctagctgctc
       61 ctgcttttcc ggcgcgaagc ggtcccaggt gcggaccatc tgcgccatgc gcggattgcg
      121 cgccagccgc tcgcggtggc gggcgatgaa gtcccagtac agcaggttga acgggcaggc
      181 tttcggcccg gccttggcct tcacgtcata agcgcaggtc cggcagtagt cgctcatccg
      241 gtcgatatag gccccgctgg cggcataggg cttactggcc agccggccgc cgtcggcaaa
      301 ctggctcatc cccacggtgt tgggcagttc gacccattcg taggcatcgg catagaccgc
      361 cagataccat tcgtgcagtt catgcggatc gatcccggcc agcagcgcga aattgccggt
      421 caccatcagc cgctggatat ggtgggcata ggcgtggtcg cgggtctgct ccacgcaggc
      481 ccgcaggcag gccatcggcg agttcgcgct ccagtagaac gacggcaacg gacggcgtgc
      541 ttccagcgcg ttgacccggt catagcccgg catccgccac cagtagatcc cgcgcacgaa
      601 ttcacgccag ccgatgattt ggcggatgaa cccttcggcg ctgttgatcg gcacccggcc
      661 ggcgcgccac tccgcctcca ccgcccggca cagcgcgagc ggatcaagca gcccggcgtt
      721 gagctggggt gagatgaagg cgtgataaag gaacggctgc cctgccagca tggcatcctg
      781 ataggccccg aactgcggca ggccgtgctt caggaaatgg gcaaaggccg cttcggcatc
      841 ggcgcgggtg acggccatgg caaagccatc cagcgagccg aagttgtccg ggaaacgcgc
      901 cgccaccaga tcgagcacgt cgcgggtgat cgcatcgggt tcgaaccgcg gcgggcgcgg
      961 catcagcagg tcggcggcgg cgggcttgcg gttttcggca tcgaagttcc agcgcccttc
     1021 ggcgggctgg tcgccctcca tcaacagccc ggtcttgcgc cgcatcaacc ggtagaaatg
     1081 ctccatccgc aggcccttgc ggccctcggc ccagtcggcg aagccgtggc ggtcggccag
     1141 gaagcggtca tcggccagca gctcggtttc tggccagccg cgcatcgcct ccagcagccg
     1201 ccattcgccc ggctcggtta cgaccacgcc ggtaagggca tggcgcgtcc gcgcccgctg
     1261 cacctctccg gccagggtgc cggtgttggc cggatcatcc agcgtgacgt agtcgacctg
     1321 ccagcccgca gcccgcagct ccgccgcaaa gtggcgcatc gcggcgaaga cgaaggcgat
     1381 cttgcgcttg tggtgcggga catagctggc ctcggccatc acttcggcca tcagcacccg
     1441 gtcccgggcc cgatcgcccg cctcgagcga agccagggtg gaagacagct ggtctcccag
     1501 gatcaggatc aggcgtccgg acatggccgc gccctaggcg gcggagcggg gcctgccagc
     1561 ctgtccgctg gggcagggcg cgggggcgcc gtctttctac tccccaagtt gacattatgc
     1621 cgcggggcgt gttctaacca tcgcccatgc tttcgcagaa aacccgctat acgatccgcg
     1681 ccttgcaaca tctggccgac acggtcgggc aggggccgat ccggctggat accattgccg
     1741 aggcgcagaa catcccgcgc aagttcctga ccgtgatcct ctcggaaatg gtccgcgccg
     1801 gcctcgtgat ctcgcaccgc gggcgcgacg gggggtacga gctggggctg gccccggtcg
     1861 acatccgcta tggcgacatc atccggctga cccggggcag cctggcgctg gtgccctgcg
     1921 ccagccggaa cgcctacgaa cactgccaga actgcctgcc agaggccgac tgccgcctgc
     1981 gcgcgctgat gctgaccctg cgggacgaaa tggccgcgat gctggaccgg gtgaccctgg
     2041 ccgatccgat caagaccgaa ccgccgttcg aggatgccga agcggggtga cttcggcttt
     2101 cggtgccgtt agaacaggcg gcgaacatgc tgactagact ggccatccgc aacgtggtgc
     2161 tgatcgaagc gctggacctc gatttcgcgg gtgggctggg cgtgctgacc ggggaaaccg
     2221 gggccggcaa gtcgatcctg ctcgatgccc tgggcctggc gctgggcgcg cgggcggaca
     2281 gcgggctggt ccgcgccgga gaggatcagg ccagcgtcac tgccagcttc gaattcgaca
     2341 agctgcccat gccgatccgt gcgatcctgg ccgaggccga ggtggatatc gagccgggcg
     2401 agccgctgct gatcaagcgt cgggtcaagg ccgatggcgg atcaaaggcc ttcgtcaacg
     2461 atcagccggt tggcgccgcc ctgctgcgcg aactcagcgg ccatctggtg gaactgcacg
     2521 gccagcacga cgatcgcggg ctggtcaatc cgcgcgggca ccggatgctg ctcgatcgct
     2581 tcgcgcgggc cgatgtggcc ggcgtggaag ccgcctggca ggcctggcgc gcggcgcagg
     2641 acgcgctcga cgcggcgcgg gccgggatcg tcaaggccgc cgaagaccgc gatctgctgc
     2701 tggcccacct gtccgaactg accgcgctgg cgcccgaagt gggcgaggag gaacagctcg
     2761 ccgaggcccg cgccacgatg caaaagggcg aaaagctgac cgacgatctg gaggcgctgc
     2821 gcaagctgtg ggccggatcg gattcggcgc tggcgagcct gcgcagcgcg gcgcggcggt
     2881 tggaccggat tgccgaggag catcccctgc tggccgaagc cctgggcgcg ctggaccgcg
     2941 cggtgatcga ggcgagcgag gccgaagaaa agctggagcg cgcggccgag gccctggccc
     3001 acgatcccgc cgcgctggac cggatcgaga cccgcctgtt cgaactgcgc gccgcggccc
     3061 gcaagcacca gtgccaggtt gacgatctgc cgcaccggat gcgcgaaatg cgcgccgcgc
     3121 tcgacgggat cgagggcggc gaggcggaac tggccgcgct ggaagcggca gccacggccg
     3181 ccgcggccga ttaccgcgcc cgggccgagg cgctctccgc cctgcggcag gaagcggcgg
     3241 cgcggctcga ttccgcggta gcgggcgaac tcgctccgct gaagctcgat gccgcgcggt
     3301 tccgcaccgc tgtcactccg cagggggagg accgctgggg cccggccggg ctcgacgcgg
     3361 tcgagttcct gatcagcacc aaccccggcg ccgatttcgc ccccctggcc aagatcgcca
     3421 gcggcggcga gctttcgcgc ttcatcctcg cgctcaaggt cgcgctggcc gaagagggcg
     3481 gggcggcgac agtgatcttc gacgaaatcg accgcggggt cggcggcgcc gtcgccagcg
     3541 cgatcggaga gcggctcgcc cggctggccg ctggcggcca actgctcgcc gtcacccaca
     3601 gtccccaggt cgccgcgcgc gggcagcagc actatgtcat cgccaagtcg agcgagggga
     3661 cggtcacccg cacctcggtc catctgctcg atgccgccgg ccgccaggaa gaaatcgccc
     3721 ggatgctgtc cggcgccgaa gtgacccccg aagcccgcgc ccaggccgac cggttgctgg
     3781 agggggtgtg atgaaactca aagacttact cttgagggct gttaagccat cgattcactc
     3841 gaagttcgat caggctattc ttcagatccc tgagtttgac gacgagtctg agcaacctga
     3901 ggccttgctt gaccatgctg agcttgagac cctgtctggt ttggctttga tcattgaata
     3961 tgaaaactcc aagaaggaga agacccagcg gatcgtgacg tgcaagcagc tggcggttca
     4021 aggtgaagcc gagtatctta aagcctattg ccaccaccgc aaagcggtcc gaacatttcg
     4081 agtcgaccga attgttgatg tctttgaccc aaagtcgggt gaatcgctca gtccagttca
     4141 agcattcttt gctcaatttt cgcccgacaa ggtcacacgc tctggcctct cttggggcct
     4201 atcggttcgc aagcgagctg acctgatcgc aatgttgaac gccctcgtgt tcattgcgcg
     4261 atgtgatcgt gactttcacc ctactgaacg tttggtcctt gaaaccgcac tctcgagctt
     4321 ttggttgcgg atggaggtgg cgggtgatcc tgatttcgac gacatacttg gctacgcaac
     4381 taggctgtca ccagatgggg agactttttg gctcgcaatg cagcggtttt ctgaagacca
     4441 aacgctgtct ggaatttttc gtcagcaggt acgcgaattg attgaagcgg acggagtcat
     4501 tcgcgaagaa gaggcctact gggcgcttga gatcgacgag tttttgagtt gccgctgatg
     4561 gagctcgtta gtcgtctcga taccgatccg gaattgatcg ctgcgatcaa acgtggctga
     4621 ccgctttgcc gctctcaacc gcccggtctg gcacatgctg accgggccgc aggccgcgct
     4681 tgcggttggc ggtcctgatg cggtgcgggt ggatccgcgc tatggcccct ttgcggcggc
     4741 gcgcgatgcc ggcgcggagg cgcaggcggc gcttgccg
//
LOCUS       DASUDV010000023         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1569139,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000023 DASUDV010000000
VERSION     DASUDV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569139"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..682
                     /locus_tag="VFV30_00490"
     CDS             41..682
                     /locus_tag="VFV30_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX4846593.1"
                     /translation="MLRKLYDWTMAKAVHPHAEWWLAAFSFVEASFFPIPPHPLLGIM
                     CLAEPKKAIRFALIATFASVAGGMLGYAIGWGLYDTLGTQILSLLGLTESFPKAACYI
                     NEYGFWIFVAKGATPIPFKLLTITAGFIGMDLLRFIAGSIISRAISFLIVGVLFRLFG
                     APIKAFIDKYLGLATASFIALVVGGFLAVTMLTGGSQEAGHKCDAVQSVADLK"
     gene            679..1320
                     /locus_tag="VFV30_00495"
     CDS             679..1320
                     /locus_tag="VFV30_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX4846594.1"
                     /translation="MSAAIRLYHCADSRSFRPLWALEELGLPYELQLMPFPPRASVEG
                     YRELNPLGTVPACFIDGELMTESAAIGHVLATRFGPSDLAVAPHEPDYPAYMNFLFMG
                     EATLTFPQTIVLRYTRLEPPERRVAQAAEDYAQWFGSRLRNAEGLLKGEFACAGRFTM
                     ADISLGYALLLAERIGLAEQVTPGMKAYFERLSARPGFQRAKAAQAAGAGLPF"
     gene            complement(1322..>2046)
                     /locus_tag="VFV30_00500"
     CDS             complement(1322..>2046)
                     /locus_tag="VFV30_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846595.1"
                     /translation="REYGAEPLGRAIAESRRAVEIAPDYGFAQAIHALTIAGAYLSLS
                     PHDPEQERAIQPHIDRAFALDPDHAGVLAAIAAASAYIGRPAEGLPRALRAIRLRPGH
                     GLAHYAAGVSSLLLSAEDDALGHFERFLQVEPESHLHYITHAWRGICRSRLHEFPAAR
                     EAFADSFALFPGNFIARLVLTCIDCIEGHRDQAADHLAAARALEPIATLQLYQARIGR
                     FFAGSALRGDALAALGSLWPEG"
BASE COUNT          332 a          676 c          686 g          352 t
ORIGIN      
        1 aagcagtgcg ccactcgaag gtcgcgacaa aggacgccaa gtgcttcgca agctttacga
       61 ctggaccatg gccaaggcgg tccaccccca tgccgaatgg tggctggccg ccttctcgtt
      121 tgtcgaggcc agcttctttc cgatcccgcc gcaccctttg ctcgggatca tgtgcctggc
      181 cgaaccgaag aaggcgatcc gctttgcgct catcgcaacc ttcgcctcgg tcgccggggg
      241 catgctgggc tatgcgatcg gctgggggct ctatgacacg ctggggacgc agatcctcag
      301 cctcctgggg ctgaccgaaa gcttccccaa ggcggcctgc tatatcaacg aatacgggtt
      361 ctggatcttc gtggccaagg gcgcgacccc gatcccgttc aagctgctga cgatcaccgc
      421 cggcttcatc ggcatggacc tgctgcgctt catcgccggc tcgatcatca gccgggcgat
      481 cagcttcctg atcgtgggcg tgctgttccg cctgttcggc gcgccgatca aggccttcat
      541 cgacaagtac ctgggcctgg caaccgccag cttcatcgcc ctggtggtgg gcgggttcct
      601 cgcggtgacc atgctgaccg gcggttcgca agaagccggg cacaagtgcg atgcggtgca
      661 aagcgtggcg gacctcaagt gagcgcggcg atccggctct atcactgcgc ggattcgcgc
      721 tcgttccgcc cgctctgggc gctggaggag ctggggctgc cctatgagct tcagctgatg
      781 ccctttccgc cgcgcgccag cgtggagggc taccgcgagc tcaatccgct aggcacggtt
      841 ccggcctgct tcatcgatgg cgaactgatg accgaaagcg cggcgatcgg ccatgtcctt
      901 gccacccgct tcggcccctc ggacctcgcg gtagcgccgc acgagcccga ttacccggcc
      961 tacatgaact tcctcttcat gggcgaggcg acgctgacct tcccgcaaac catcgtgctg
     1021 cgctacaccc ggctcgagcc gcccgaacgg cgcgtggccc aggcggcaga ggactatgcc
     1081 caatggtttg gatcgcggct gcgcaacgcc gaagggctgc tgaagggcga atttgcctgc
     1141 gccgggcgct tcaccatggc cgatatctcg ctgggctatg ccctgctgct ggccgagcgg
     1201 attgggctcg ccgaacaggt taccccggga atgaaggctt atttcgaacg gctcagcgca
     1261 aggcccggat tccagcgggc caaggcggcg caggcggccg gtgccggcct gccgttctga
     1321 atcaaccctc cggccacagg ctgccgagcg cggccagggc atcgccgcgc agtgccgatc
     1381 cggcaaagaa ccggccgatc cgcgcctggt agagctgcaa ggtggcaatc ggttcaagcg
     1441 cccgggccgc cgccaggtgg tctgccgcct gatcgcggtg cccctctatg caatcgatgc
     1501 aggtcaacac caggcgggca atgaagttgc ccggaaacag ggcaaagcta tcggcaaagg
     1561 cctcgcgcgc cgcggggaac tcgtgcagcc gcgaacggca gatcccgcgc caggcatggg
     1621 tgatgtagtg caggtgcgat tccggctcca cctgcaggaa ccgttcgaaa tgtcccaggg
     1681 catcgtcttc ggcgcttagc agcaggctgc tgacacccgc ggcataatgg gccaggccat
     1741 ggccgggacg cagcctgatc gcgcgcagcg cgcggggcag gccttctgcc gggcggccga
     1801 tataggcgct tgccgcggcg attgccgcca gcaccccggc gtgatcggga tcgagcgcaa
     1861 aggcccggtc gatgtgcggc tggatcgcgc gttcctgttc gggatcgtgc gggctgagtg
     1921 aaagataggc gccggcgatg gtcagggcat ggatggcctg ggcaaagccg tagtcgggcg
     1981 cgatctccac cgcgcggcgg gattcggcga tcgcccggcc gagcggctcg gcgccgtatt
     2041 cgcggt
//
LOCUS       DASUDV010000024         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2187790,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000024 DASUDV010000000
VERSION     DASUDV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187790"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFV30_00505"
     CDS             complement(<1..679)
                     /locus_tag="VFV30_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4846596.1"
                     /translation="MPAFRTALVLSLALAACAPAAPPRALTAADYAAVLADPVRPAAD
                     RARDEARKPGELLAFAEIAPGETVGDFVMGGGYFTRVLSAAVGPKGRVEAFQPAEFIA
                     YRAAYGEEQRSTVTALSNSVAIAGPFAAPAFTRPLDTIVTIQNFHDLYLKPFPAGTGA
                     KASAALFAALKPGGTLVVVDHSAVAGSGTRDADTLHRIEKAAVIEALTAAGFKLEAEA
                     PLYVRPED"
     gene            complement(744..1748)
                     /gene="bla"
                     /locus_tag="VFV30_00510"
     CDS             complement(744..1748)
                     /gene="bla"
                     /locus_tag="VFV30_00510"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX4846597.1"
                     /translation="MISAIALPAGAAQAENFQAEFDKVFGKELRAPREVQPTVRPFIA
                     PARPVTTYGGSLEATVASLADAAQGRIGVAAMDLGTGRTVSVLGDQPFPMASTSKIAI
                     AATYLDLVDQGKLSLSDRFPLMIPQRSARFSTPVAPVRPGTMMSAQGLIELSITRSDN
                     PATDALLAAVGGPAAVNRWMRKAGLSGMRLDRDIATLVRDDGEFNPATTVDLRDSATP
                     LAMVQLLAGLHQGRWLSASSRSVLLGAMERCVTGKRRLPAMLPQDARVAHKTGTLNNT
                     SSDVGIINTPDGRAIAIAVYVTGQGGKPNRDYRIAAISRAVYDGYLTESSSLRRTASR
                     "
     gene            complement(1690..2100)
                     /locus_tag="VFV30_00515"
     CDS             complement(1690..2100)
                     /locus_tag="VFV30_00515"
                     /inference="COORDINATES: protein motif:HMM:NF037786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846598.1"
                     /translation="MVSGPPAPGKAFNPAMVVELLRRLAAAGIAFIKTEQLDLFDGQP
                     GFSLGQDQQPERISQGVPACKNYTGTPHFLASGRQLVARRVDGRVALDNLKPCLGYRC
                     AGEEHPLPSSSGRTGHDQRDRVARRRGASGKFPG"
     gene            complement(2131..>2325)
                     /locus_tag="VFV30_00520"
     CDS             complement(2131..>2325)
                     /locus_tag="VFV30_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846599.1"
                     /translation="DRIIGAKQASFFIGRNSLALGGSSKLPRWQRILFVILHRNAADP
                     TDYFEIPPNRMIELGTQYTI"
BASE COUNT          383 a          728 c          835 g          379 t
ORIGIN      
        1 gatcttcggg ccggacatag agcggggctt ccgcttccag cttgaacccg gcggcggtaa
       61 gcgcctcgat cacggcggcc ttctcgatcc ggtggagcgt atcggcatcg cgcgtgcccg
      121 atccggcaac cgcagagtga tcgaccacca ccagcgtgcc gccgggcttg agcgcggcaa
      181 acagcgcggc gctggccttg gccccggtcc cggccgggaa gggcttgagg tagagatcgt
      241 ggaagttctg gatcgtcacg atcgtatcga gcgggcgggt aaaggccggg gccgcgaagg
      301 gcccggcgat cgccacgcta ttggacagcg cggtaacggt gctacgctgc tcctcgccat
      361 aggcggcgcg ataggcgatg aattcggccg gctggaacgc ctcgacgcgc cccttcgggc
      421 cgaccgcggc ggagaggacg cgggtgaaat agccgccgcc catcacgaaa tcgccgacag
      481 tctcgcccgg cgcgatctcg gcaaaggcaa gcagttcgcc cggtttgcgg gcttcgtcgc
      541 gggcgcggtc ggcagcgggg cggaccggat cggcgagcac ggcggcataa tcggccgcgg
      601 tcagcgcgcg tggcggcgcg gccggggcgc aggcggcgag ggccagggac agaaccaggg
      661 cagtgcggaa ggcgggcatg gcgagtctcc tctcagggag tgccatggct aggcccgttg
      721 gggcgtaagc gccagagcct cgctcagcgc gaggcggtgc ggcgcaggct ggagctttcg
      781 gtcaggtagc cgtcatagac cgcgcggctg attgcggcga tccgatagtc gcggttgggc
      841 ttgccgccct ggccggtgac gtagacggca atcgcaatcg cgcggccgtc gggcgtgttg
      901 atgatcccga cgtcgctcga ggtgttgttc agcgtgccgg tcttgtgggc aacgcgtgcg
      961 tcctgcggca gcatggccgg caggcggcgc ttgccggtga cgcagcgttc catcgcgccg
     1021 agcagcacgc tgcggctcga agcgctgagc cagcggccct ggtgcaggcc ggcgagcagc
     1081 tgaaccatcg ccagcggcgt ggcgctgtcg cgcaggtcaa cagtggtcgc cggattgaac
     1141 tcgccatcgt cgcgcaccag cgtcgcgatg tcgcggtcaa gccgcatgcc cgaaagaccg
     1201 gccttgcgca tccagcgatt gaccgccgcc ggaccgccaa cggcagccag cagggcatcg
     1261 gtggcgggat tgtcgctgcg ggtgatcgag agttcgatca gcccctgcgc gctcatcatc
     1321 gtgcccgggc gaaccggcgc caccggggtc gagaagcgcg ccgaacgctg cgggatcatc
     1381 agtgggaagc gatcgctgag cgacagcttg ccctgatcga ccaggtccag ataggtcgcg
     1441 gcaatcgcaa tcttgctggt gctggccatc gggaacggct ggtcacccag tacggaaacg
     1501 gtgcggccgg tgcccaggtc catcgctgcc acaccgatcc ggccttgtgc agcatcggcc
     1561 aggctggcga cggttgcttc aaggctgccg ccataggtcg tgacggggcg ggctggggcg
     1621 atgaaggggc ggacagtcgg ctgaacctcg cgcggggcgc gcagttcctt gccaaagacc
     1681 ttgtcgaatt cagcctggaa attttccgct tgcgccgcgc ctgcgggcaa cgcgatcgcg
     1741 ctgatcatgg ccagtccggc cagaagacga gggaagcggg tgttcttcac cggcgcagcg
     1801 atagcccaaa catggtttca gattgtctaa cgcgactcgc ccgtccaccc gccgcgcgac
     1861 gagttgacgg cctgatgcga ggaaatgagg ggtacctgtg taattcttgc aggcaggtac
     1921 cccctgtgag attctttcag gttgctggtc ctggcccagc gagaagcccg gttggccatc
     1981 gaacaggtcg agctgctcgg tcttgatgaa cgcgatcccg gctgcggcca gccgccgcag
     2041 cagctcgacc accatcgccg gattgaaggc cttgccgggt gctggcggtc cgctgaccac
     2101 cggcacccag tcccctgcca ctagctggca tcagatggtg tattgggtgc ccagctcgat
     2161 catccggttg ggtggaatct cgaaatagtc agtcggatcc gccgcattgc gatggaggat
     2221 gacaaacagg atccgttgcc aacgcggtag ttttgagctg ccgcccagcg ccagactgtt
     2281 gcggccgatg aagaagctgg cctgcttcgc gccgatgatc cggtc
//
LOCUS       DASUDV010000025         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1348428,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000025 DASUDV010000000
VERSION     DASUDV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348428"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFV30_00525"
     CDS             complement(<1..1077)
                     /locus_tag="VFV30_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX4846600.1"
                     /translation="MQRNLKSVDWDNLPHGGDLFGELQLTDTAPPPAKRPPLFPGLAL
                     CGVAAAAAAWLSDHYGFPIILLGLLVGLALNFVSQDERTHAGLDFASRTCLRWGIVVL
                     GLQVTFAQIGAVGVAPFAALGVVLATTLLAGLAGARLAGQSRYAGILAGGATAICGAS
                     AALALYGVIGKERLSQAQFALTLVGVSLASALAMTVYPMIATQLNLTDAQAGFLIGAS
                     IHDVAQAIGGGYAVSDAAGAQATIVKLARVALLAPVVALTSLWIGAAGEAKDQPVWRR
                     LALPWFILAFLGMVTLNSLITIPADLAKNGLVLSKGLLLLAVTATAMRSRMDLLLEVG
                     WRAMIPVATATVVSFVCALGFAWAL"
     gene            complement(1186..>2542)
                     /locus_tag="VFV30_00530"
     CDS             complement(1186..>2542)
                     /locus_tag="VFV30_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4846601.1"
                     /translation="GGGTVWDSITYDPVTDLVYFGTANAEPWNPAYRNTEGAGDSLYT
                     ASIVAVKPDTGEYVWHFQETPEDRWDFDSNQQITVADLEIGGQKRHVIMHAPKNGFFY
                     VLDAKTGEFISGKPFVDGINWAKGLDPKTGKPDVNPEAQYERTGKPFLGFPGAVGAHS
                     WSPMSFSPKTGLVYIPTNNTPQVYAHDPDWKPGTTGFQLGIDATPGNVPADKAIREAT
                     KKAMTGALTAFDPVSGTVKWRVPLETPTNGGTLATAGNLVFQGTALGEFRAYAADTGK
                     QLWAFPTQSGILAAPMTYMIDGEQYVAVMVGWGGVWDVSAGKGVTTRITPNVSRLMVF
                     KLGAKGQLPPLAASAERLLDPPPLSGTPEQLAMGEKLYTNSCSVCHGATAVAGALNPD
                     LRHSVSLGNPKLWQDIVHDGLLKQNGMVGWKGQFNPDQIEAIRLYVIKRANEDKALGD
                     H"
BASE COUNT          401 a          868 c          873 g          400 t
ORIGIN      
        1 cagcgcccag gcaaagccca gcgcgcagac gaagctgacc acggtggccg tcgccaccgg
       61 gatcatcgcc cgccagccca cttccagcag caggtccatc cggctgcgca tcgccgtggc
      121 ggtcaccgcc agcagcagca gccccttgga gaggactagc ccgttcttgg cgagatccgc
      181 cgggatcgta atgaggctgt tgagcgtcac catgcccagg aaggccagga tgaaccaggg
      241 cagggccaga cgccgccaga ccggctggtc cttggcttcg ccggcggcgc cgatccacag
      301 gctggtcagc gccacgaccg gggccagcag cgccacccgg gccagcttga cgatggtcgc
      361 ctgggccccc gccgcgtccg acacggcata gccgccaccg atcgcctggg cgacatcgtg
      421 gatcgaggcg ccgatcagga acccggcctg ggcatcggtc aggttgagct gggtggcgat
      481 catcggatag actgtcatgg cgagcgcgct ggccagcgag acacccacca gggtcagcgc
      541 gaactgcgcc tggctgagcc gctccttgcc gatcacgcca tagagcgcca gcgccgcgct
      601 ggccccgcag atcgcggttg ccccgccggc caggatcccg gcatagcgcg attgcccggc
      661 gagccgcgcc ccggccagcc cggccagcag cgtggtggcc aggaccaccc ccagcgcggc
      721 aaagggcgcc acgcccaccg cgccgatctg cgcgaaagtg acctgcaggc ccaggacgac
      781 gatcccccag cgcaggcagg tgcgcgaggc gaaatcgagc ccggcgtggg tgcgttcgtc
      841 ctgcgagacg aagttcagcg ccaggccgac cagcaggccc agcaggatga tcggaaagcc
      901 atagtggtcc gaaagccagg ccgccgccgc ggccgcaacc ccgcacagcg ccaggccggg
      961 aaacagcggc gggcgcttgg ccgggggcgg cgcggtgtcc gtaagctgca gctcgccgaa
     1021 caggtcgccg ccgtggggca ggttgtccca atcgactgat ttcagattgc gctgcatcgt
     1081 tccccgaccc ggaccccgtt cctgtcctgc ggtgtcatgc ctgaaatccg ccgggaacgg
     1141 aagcccggtc ccggcggaat atcatgccct atgccaaggg ccgggctagt gatcgcccag
     1201 cgccttgtcc tcgttggcgc gcttgatcac gtagagccgg atcgcctcga tctggtccgg
     1261 gttgaactgg cccttccagc cgaccatccc gttctgcttg agcaggccgt catggacgat
     1321 gtcctgccac agcttgggat tgccgagcga gaccgagtgg cgcagatcgg ggttcagcgc
     1381 gccggccacc gccgtggcgc cgtggcagac cgagcagctg ttggtgtaga gcttttcgcc
     1441 catggccagc tgttccggcg tgcccgaaag cggcggcgga tcgagcaggc gctccgcgct
     1501 ggcggccagc gggggcagct gccccttggc gcctagcttg aacaccatca gccggctgac
     1561 gttgggggtg atccgggtgg tcacgccctt gccggccgac acgtcccaca cgccgcccca
     1621 gccaaccatc acggcgacat actgctcgcc gtcgatcatg taggtcatcg gcgcggcgag
     1681 gatcccgctc tgggtcggga aggcccacag ctgcttgccg gtatcggcgg cataggcgcg
     1741 gaattcgccc agcgcggtgc cctggaaaac caggttcccg gccgtggcca gggtcccgcc
     1801 gttggtcggc gtttccagcg gcacgcgcca cttgacagtg ccggaaaccg gatcgaacgc
     1861 cgtcagcgcg ccggtcatcg ccttcttggt ggcttcgcgg atcgccttgt cggccgggac
     1921 gttgcccggg gtggcgtcga tccccagctg gaacccggtg gtgcccggct tccagtccgg
     1981 atcgtgggca tagacctgcg gcgtgttgtt ggtcgggata tagaccagcc cggtcttggg
     2041 gctgaagctc atcggcgacc agctgtgcgc gccgaccgcg ccggggaagc ccaggaacgg
     2101 cttgccggtg cgctcgtact gcgcctcggg gttcacatcg ggcttgccgg tcttgggatc
     2161 gaggcccttg gcccagttga tcccgtcgac gaagggcttg ccggaaatga actcgccggt
     2221 cttggcgtcg agcacataga agaagccgtt cttcggcgcg tgcatgatca cgtggcgctt
     2281 ctgcccgccg atttcgaggt cggcgacggt gatctgctgg ttggaatcga agtcccagcg
     2341 gtcttccggg gtttcctgga agtgccagac atattcgccg gtgtccggct tcaccgccac
     2401 gatcgaggcg gtgtagagac tgtcgcccgc gccttcggtg ttgcgatagg ccgggttcca
     2461 cggctcggca ttggccgtgc cgaagtaaac cagatcggtg acgggatcgt aggtgatcga
     2521 atcccacacc gtgccgccgc cg
//
LOCUS       DASUDV010000026         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2187872,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000026 DASUDV010000000
VERSION     DASUDV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187872"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFV30_00535"
     CDS             <1..270
                     /locus_tag="VFV30_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX4846602.1"
                     /translation="VGKQEIFERLLYPMVSEGALILEEGIAQRASDIDLVWLNGYGWP
                     AWTGGIMFWADHEGLAKIVAGLKRHNLPVAPLLEKLASTGGSFNA"
     gene            267..899
                     /locus_tag="VFV30_00540"
     CDS             267..899
                     /locus_tag="VFV30_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX4846603.1"
                     /translation="MSTATALFAFTGTAALLTITPGLDTALVLRTAAVEGGRSALLAA
                     LGIAAGCLAWGLAAGVGLGALLAASQLAYEALRWVGAGYLLLLGVRLIRAPRNALDLA
                     SATNQADTAAPNWLVRGFLTNLLNPKVGLFYVSFLPQFIPAGSSVLGWSIMLASIHNV
                     LGLIWFAALIAATRPLTAALQRPAVIAWLDRITGAIFVAFAARLALASRG"
     gene            1033..>2025
                     /locus_tag="VFV30_00545"
     CDS             1033..>2025
                     /locus_tag="VFV30_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846604.1"
                     /translation="MGREVKSKILDGINDLVIMAPIREGFIDAYEPVTYATRLRIICE
                     ALNDIRAAAREYEKDVPFADASERILSLLDFKAGIIDNDLLQLQPGMGLSARRYFYLI
                     ATFDGVWEPYMRLIWNPLGSLLDLLFANCDGYVFACDNSFEDYINWVRSAQVDSAVFF
                     TTTGVTVADQLYLRNLEKLHRESEPAAGDLAITRMAVRNPGDVAGEWREASLGNTFAA
                     HRLALEALGVFYRLADYYPPLPAEVLDHIGDVYRADGYLLQRAMHRILDGWRHESLQP
                     LLADGGILARFRPLVEWFASGPIHPDFASQQRPVAEPVLDHSQIQRGILSEPKLP"
BASE COUNT          336 a          636 c          643 g          410 t
ORIGIN      
        1 gtcggcaagc aggaaatctt cgaacgcctg ctctatccga tggtctcgga aggcgccctg
       61 atcctcgagg aagggattgc ccagcgcgcc agcgatatcg atctggtctg gctcaacggc
      121 tatggctggc cggcctggac cggcgggatc atgttctggg ccgatcacga agggctcgcc
      181 aagatcgtcg ccggactgaa gcgccataac ctgccggtgg cgccgctgct tgaaaagctg
      241 gccagtactg gcggcagctt caacgcttga gcacggccac cgccctcttt gccttcaccg
      301 gcactgcagc attgcttacc atcacgccgg ggctcgatac cgcactggtg ctgcgcaccg
      361 ccgcggtgga aggggggcgc agtgccttgc ttgcggcgct ggggattgcc gcaggctgtt
      421 tggcctgggg gcttgcggcg ggagtcgggc ttggcgcgct gctcgctgca tcgcagctgg
      481 cctatgaggc gctgcgctgg gtgggcgcgg ggtatctgtt actgctaggg gtgcggttga
      541 tcagggcgcc gcgcaacgcg ctcgatctgg catcggccac caaccaggct gatacggctg
      601 cgcccaactg gctggtccgc ggcttcctga ccaacctgct caaccccaag gtcgggcttt
      661 tctatgtctc gttcctgccg caattcattc ccgccggaag cagcgtgctg ggctggtcga
      721 tcatgctcgc ctcgatccac aacgtgcttg gcctgatctg gttcgcggcg ctgatcgcgg
      781 cgacccggcc attgaccgca gccttgcagc gccccgccgt gatcgcatgg cttgaccgga
      841 tcaccggcgc gattttcgtc gccttcgcag ccaggctcgc actcgcctcg cgcggctgaa
      901 ctgctcccgc agcctgtggg gtttcgcaca gaaattactg gcgcgattgc cgacaaccgc
      961 ctgtcatgcc agcattcgca gcgggacggg caagtgttgg cgcgagctct cgacaaatga
     1021 ggaggggcaa ggatgggccg cgaagtcaaa agcaagatcc tcgatggcat caacgatctg
     1081 gtgatcatgg cgccgatcag ggaaggcttc atcgatgcct atgaaccggt cacctatgcc
     1141 acgcggctgc ggatcatttg cgaagctctg aacgatatcc gcgccgccgc ccgcgaatat
     1201 gaaaaggacg tcccctttgc cgatgcgtcc gaacgcatcc tttcgctgct cgatttcaag
     1261 gccgggatca tcgacaacga tctgttgcag ttgcagcccg gcatggggct gagcgcgcgg
     1321 cgctatttct acctgatcgc cacgttcgat ggggtgtggg aaccctacat gcggctgatc
     1381 tggaatccgc tgggatcact gctcgatctg ctgtttgcca attgtgatgg ctatgtcttt
     1441 gcctgcgaca acagtttcga agactatatc aactgggtcc gcagcgcgca ggtggacagt
     1501 gcggtgttct ttaccaccac cggggtcacg gttgcggacc agctttacct gcgcaacctt
     1561 gaaaaattgc accgcgaaag cgaaccggca gcgggcgatc ttgccatcac ccggatggcc
     1621 gtgcgcaatc cgggcgatgt cgccggggaa tggcgcgagg cttcgctggg caataccttt
     1681 gccgctcacc gcttggcgct agaggcgctg ggggtgttct accggcttgc cgattactac
     1741 ccgcctcttc ccgccgaagt tcttgaccat atcggcgatg tttaccgggc cgacggctat
     1801 ttgctccagc gggcgatgca ccgcattctc gatggctggc ggcacgagag cctgcaaccg
     1861 ttactcgccg acggcggcat tctcgcgcgg ttccgcccgc tcgtggagtg gtttgcgagc
     1921 gggccgatcc accccgattt tgcctcgcag caacggccgg tcgcagaacc cgtgctggac
     1981 cattcgcaga tccagcgtgg catcctgagc gagccgaagc tgccg
//
LOCUS       DASUDV010000027         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_508960,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000027 DASUDV010000000
VERSION     DASUDV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508960"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFV30_00550"
     CDS             complement(<1..795)
                     /locus_tag="VFV30_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX4846605.1"
                     /translation="MSDAAQPPMPRGGDLTQGPLARTLLVFALPQLIGNVLQSLNGSI
                     NAVWVGQLLGDQALAATANANIIMFLLFALVFGFGMAATVRIGQAWGAKDVVAARRVL
                     GAALGLTGAITLVTSIAGFVYAPVLLDWLATPGAARQQAQIYLQVVFIANPISTISMM
                     IAMGLRGAGDAATPLRFMILTTLLDIVLNPLLILGWGPFPALGIAGSAWATVIATSIG
                     FAAMLAWIYAKDLPLRLRGAELRWLRPAAAEARYLLAKGLPMGAQMV"
     gene            complement(792..1523)
                     /locus_tag="VFV30_00555"
     CDS             complement(792..1523)
                     /locus_tag="VFV30_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX4846606.1"
                     /translation="MLTLATTTPGEPEIFASLQGEGPSLGRPVAFVRLSRCNLACTWC
                     DTAYTWRFEGDNRPHRDGLAFERSANQVVLDEAEVAARIRALGQDRLVITGGEPLLQG
                     AALARMVALLDGISVEIETNGTAAPHPALDALVAQYNVSPKLAHSGNPAELALLPERL
                     AAWAADPRAFFKFVIAAPEDLAEVLALIETHGMDRARVFVMPEGTDSATLRTRERWLA
                     PLALEHGLRLSDRLHIHLYGDTRGT"
     gene            complement(1906..>2557)
                     /locus_tag="VFV30_00560"
     CDS             complement(1906..>2557)
                     /locus_tag="VFV30_00560"
                     /inference="COORDINATES: protein motif:HMM:NF021532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2262 domain-containing protein"
                     /protein_id="HEX4846607.1"
                     /translation="DGGAIEAAQLRLILGNLDRETAQAFAQTWAIGALVALRVDAAPD
                     PDAPTPQLFAVSFDGAVSDPELADCTVPAPSYPPFEDSRFGTFTRAGEYASYQASAVW
                     IGRTVTLELDATGPALPQAAAHAAALFDQAQLWQAELDDCLVRDLHSPWNETWKGDRP
                     ALARQDWLSRITLTRISIDPTGAFLAYFSDGDLFWGHAIEVSGSRDAGASEARLLG"
BASE COUNT          415 a          904 c          893 g          345 t
ORIGIN      
        1 caccatctgc gcccccatcg gcaggccctt ggcgaggaga tagcgcgcct ccgcggccgc
       61 cggccgcagc cagcgcagct cggccccgcg cagccgcagc ggcaggtcct tggcatagat
      121 ccaggccagc atcgcggcaa agccgatgct ggtggcgatc accgtcgccc aggccgatcc
      181 agcgatgccg agcgccggaa acgggcccca gcccaggatc agcagcgggt tgaggacaat
      241 gtccagcagc gtggtcagga tcatgaagcg cagcggcgtg gccgcatcgc cggccccgcg
      301 caggcccatc gcgatcatca tgctgatggt cgagatcgga ttggcgatga acaccacctg
      361 cagatagatc tgcgcctgct gccgcgccgc gccgggtgtt gccagccagt ccagcagcac
      421 cggggcatag acgaacccgg cgatggaggt gaccagcgtg atcgccccgg tcaggcccag
      481 cgccgccccc agcacccgcc gcgctgcgac cacgtccttg gcgccccagg cctggccgat
      541 ccgcaccgtc gcggccatgc caaagccgaa caccagcgcg aacagcagga acatgatgat
      601 gttggcattg gccgtggcgg ccagcgcctg atcgcccagc aattgcccca cccagaccgc
      661 gttgatcgat ccgttgaggc tttgcaggac gttgccgatc agttgcggca aggcaaagac
      721 cagcagcgtc cgcgccagcg gcccctgggt caggtcaccg ccgcgcggca tcgggggctg
      781 ggcggcatcg ctcacgtgcc gcgggtgtcc ccgtaaaggt ggatgtggag ccggtcgctc
      841 agccgcaggc cgtgctccag cgccagcggg gcgagccagc gctcgcgcgt ccgcagggtc
      901 gcgctgtcgg tgccttcggg catgacgaac acgcgcgccc ggtccatgcc gtgggtttcg
      961 atcagggcca gaacctcggc caggtcctcc ggcgcggcga tcacgaattt gaagaacgcg
     1021 cgcggatcgg cggcccaggc ggccagccgt tcgggcagca gcgccagttc ggccggattg
     1081 cccgaatggg ccagcttggg gctgacgttg tactgcgcca ccagggcgtc gagcgcgggg
     1141 tgcggcgccg ccgtgccgtt ggtctcgatt tccacgctga tcccgtccag cagcgccacc
     1201 atccgcgcca gcgccgcgcc ctgcagcagc ggctcgcccc cggtgatcac cagccggtcc
     1261 tgccccagcg cccggatccg cgcggcgacc tcggcctcgt ccagcaccac ctggttggcg
     1321 ctgcgctcaa aggccagccc gtcgcggtgc ggacggttgt cgccctcgaa ccgccaggtg
     1381 taggccgtgt cgcaccaggt gcaggccagg ttgcagcgcg acagccggac aaaggccacc
     1441 gggcggccca gcgaaggccc ttcgccctgc agcgacgcga agatttccgg ttcgcccggc
     1501 gtggtggtgg caagggtcag catgcggcag cgtcacgctc aggttgacac agttgacact
     1561 gcgtccgctt gttccaacag cccgcaactc gcggaaaacc gaccgaaagg ctgccacaca
     1621 ggccggccgc gtccgcgtgg gcggaaaaaa gcggcgcgag gtaggcaggg gcggagagca
     1681 tggcgcagcg tccttggcag atttgccggg agtaggacag gcccttcctc cccggaacgg
     1741 ggagggggac cagcgaagct ggtggagggg cacgtgcggg tgatcgctgc ctctccggcc
     1801 aggtcaggac cactgccgcg tgcccctcca ccatcctgcg gatggtcccc ctcccccaag
     1861 ggggaggaat gctaccccag ccgcgccagg gccgcggcca gccgctcagc ccagcaggcg
     1921 ggcctcggat gcaccggcat cgcgcgatcc gctgacttcg atcgcgtgac cccagaacag
     1981 atcgccgtcg ctgaaatagg cgaggaacgc gccggtgggg tcgatgctga tccgggtcag
     2041 ggttatccgg ctgagccaat cttgccgggc cagcgcgggc cggtcgccct tccaggtctc
     2101 gttccacggc gaatggagat cgcggaccag gcagtcgtcc agctcggcct gccacaactg
     2161 ggcctgatcg aacagcgcgg cagcgtgggc ggcagcctgc ggcagggccg gcccggtcgc
     2221 atccagttcc agcgtgacgg tccggccgat ccagacggcg gacgcctggt aagatgcgta
     2281 ttcgccggcc cgcgtgaaag tgccgaaccg gctgtcctcg aagggcggat aggacggcgc
     2341 gggcacggtg cagtccgcca gttccggatc gctcaccgcc ccatcgaacg aaacggcgaa
     2401 aagctggggc gtaggtgcgt ccgggtccgg ggcggcgtcc acccgcagcg cgacgagcgc
     2461 gccgatggcc caggtctgcg caaaggcctg cgcggtttcc cggtcaagat tgcccaggat
     2521 cagccgcagc tgcgcggctt cgatcgcgcc cccgtca
//
LOCUS       DASUDV010000028        11517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_906603,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000028 DASUDV010000000
VERSION     DASUDV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906603"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFV30_00565"
     CDS             complement(<1..625)
                     /locus_tag="VFV30_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX4846608.1"
                     /translation="MSEIEEGPVSLSEAPTKRLNMIEAINEALHIMMERDPDVVVFGE
                     DVGYFGGVFRATAGLQKKYGKNRVFDTPINECGIIGAAVGMGAYGLRPVPEIQFADYI
                     YPGLDQLVSEAARLRYRSAGEFIAPITVRSPFGGGIFGGQTHSQSPEALFTHVAGLKT
                     VIPSTPHDAKGLLIAAIEDNDPVIFFEPKRIYNGPFSGYYDKPVEPWA"
     gene            complement(622..1923)
                     /locus_tag="VFV30_00570"
     CDS             complement(622..1923)
                     /locus_tag="VFV30_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEX4846609.1"
                     /translation="MAQGPESAGTAGGNLPPLNLHVPEPPFRPGDPVDYSFLDIPPAG
                     AFARPDEACPTEETHVLCHGLIRVLDEDHQAVGAWDPKLDPETLRRMLRTMALTRAFD
                     DRMYRGQRQGKTSFYMKCTGEEATSVASAFALSDDDMVFPSYRQQGILIARGYPIVEM
                     VNQIYSNRADKLKGRQLPIMYSSREHNFFTISGNLATQMPQAAGWAMASAIKGDTRIA
                     STWIGEGSSAEGDAHAAFIFATVFNAPVVFNVVNNQWAISSFSGFAGGERATFAARAI
                     GYGIAGLRVDGNDALAVYAAARWAANRARAGKGPTLIEHFSYRAEGHSTSDDPSAYRS
                     AHEREEWPLGDPIMRLKRHLIALGEWSVEQHEAMDLELAEQVKAATKEAEKNGVLGHG
                     LHHPFHTMFEDVYEELPWHLEEQADQAMRERRIKWPEWKEP"
     gene            2165..3334
                     /locus_tag="VFV30_00575"
     CDS             2165..3334
                     /locus_tag="VFV30_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX4846610.1"
                     /translation="MRWKTCLLALPLVLAAPVLSAQDEPQDASPETAQDDPRVLDPEN
                     DPEVFAAVSQNFTRSVNAPCGYGAARCPDPMTVESEDTVLADNLLPPDAKPIKPKPPK
                     PEKQRFTRVMQDGKWVKVPIPKKNKTRVGNGFIAPPGSAPWMAQIQRPLYVKAATARA
                     LDWEDRQFCGGALIAPGWIVTAAHCLTEYKSNIRAAGYRVRLGTNDIKTAQQAVSYRI
                     VSIHEHPDYDAGNYSNDIALVRFAADEQTNRDARAWIQAIAVDGDSPGTSRIEGREAY
                     FYGWGLTGNGRPSSTLLFGKIRLEPDSRCPKSMIALCGRGIGAKGSTQCHGDSGGPLV
                     LFNGRTPMLVGVVSHNIGKQACGNNVKQGVFTRIASYRKWIEGFTGPLRRPASRR"
     gene            3331..4392
                     /locus_tag="VFV30_00580"
     CDS             3331..4392
                     /locus_tag="VFV30_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX4846611.1"
                     /translation="MSGPGARLTFAALLAVLGTGTLPAQDTPSQPPLCDDQGPCADPA
                     ALDSASLPMTTVAASPDDYVPKPEPAPSPENPTGQSYAVGGGFVLTDHTAPWMAQIRR
                     PERMTGITQRRLTWSERHQCSGAVIKPGWVLTAAHCLTDTLDLRGNTANIRQLNYRVW
                     LGVKDIGKDRSGTSYRIVGIYHPQSYRAGSYSSDIALVQYAVDPETDLSRGVRASAIA
                     VDRLPPDKRRFGNDVVHFYGWGKTERDAVSGPLLFGKAVVAPDDQCKWPSIAICGLQI
                     AKIGIPGRQSTQCHGDSGGPLVWFSKRRVPLLIGVVSHNVGKATCGTQRTAGVFTRAS
                     AFVGWIEGVAGPLPPAPPL"
     gene            complement(4445..4657)
                     /locus_tag="VFV30_00585"
     CDS             complement(4445..4657)
                     /locus_tag="VFV30_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846612.1"
                     /translation="MRKLTTGLLVAASALALAGCEKKEEAPAATTEASTEASAAASDD
                     AGAATATDAAATATETNDPNGNPIKP"
     gene            complement(4690..6642)
                     /locus_tag="VFV30_00590"
     CDS             complement(4690..6642)
                     /locus_tag="VFV30_00590"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX4846613.1"
                     /translation="MNSVTDADGAAGSRPSVFLSYARADQKRARQVIGVLEQAGLRVW
                     WDGLLEGGETFLPTTEAALESADAVVVLWTRTSVDSHWVRDEATRGRERGCLVPVSLD
                     GSHAPLGFRQFQAINLQKWRGKADAPEIQAVIRAVLGFAGQPHPAPAARTAKGPTRRG
                     LLIGAGTAAAALAGGFALWRGLAPGAAQAAENTIAVIPFTNLGATENYFSAGLSEELR
                     ATLARNRMLRIAAPTSSAEFRGDKGDDVLTIAKRLGVAHVLRGSVRQEAGQIRVSVEL
                     LDGHDAGVDWSVQLDRQASDVLALQTEIAVAVSKALASAISALAVAEGRSGDLALLGG
                     TGNAKAFDAFLRGKALADQSKDEATDRAALERFDAAVALDGKYARALAARAKILAVIA
                     NETGQSAEIPGLYDRSVQSAEAAIRAAPELAEAHTALGYTLYNGRLDPKAARAPYDRS
                     RELGAGDADVLRTFALYTAYTGRPAEAAEAIGTALELDPLNHGAFRAAGYIAYARRDF
                     ADTVVQMRKALELSPKLSAVNSAIGTALYLQGQLADADAAFAAEPLELFRLQGQAMVR
                     QRTGDKAGAQQALTLLSSRYGRNSNYQQAQVLAQWGDRAGALDRLEKAAAARDAGLLL
                     AGADALLDPVRSDPRFAELLSRLGLS"
     gene            complement(6707..7663)
                     /gene="thyA"
                     /locus_tag="VFV30_00595"
     CDS             complement(6707..7663)
                     /gene="thyA"
                     /locus_tag="VFV30_00595"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX4846614.1"
                     /translation="MASPAIPLDSARDNHWEWQYLDLMRRIWTQGDERIDRTGVGTRA
                     VFGAQIRFDLAGGAMPLITTKRIYWKTSAREFLWFLTGNTNIRPLVEQGVKIWNEWPH
                     ANYVKATGDAISVEAFAERIAADAAFAAQWGDLGPVYGKQWVDWPTYQPAGDGLFRPG
                     PGINQVAQVVESLRGNPGSRRHIVEGWNVAELDRMALPPCHKTYQFQVAGGKLSCLLY
                     QRSCDVALGLPFNLFGAALFTRMLAQQCDLEPGELVWMGGDTHLYLNHADLVEAQLAR
                     TPAGGGNMVILRRPASIFDYRIEDFEVTGYAPQGHIAAPVAV"
     gene            7763..7839
                     /locus_tag="VFV30_00600"
     tRNA            7763..7839
                     /locus_tag="VFV30_00600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7797..7799,aa:Pro,seq:cgg)
     gene            complement(7862..9004)
                     /locus_tag="VFV30_00605"
     CDS             complement(7862..9004)
                     /locus_tag="VFV30_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4846615.1"
                     /translation="MKRSLLALTILLTGATDGLIHKPVWTPSTIATLRAWVSAAPDEG
                     LPRPDSAALDEAIAGGSTVMIDDAATKLALKLARMRLLGTASAAERTGWRIADTDRAI
                     DVEAQLQAALAAGRLDEFFAGQRPQHPEYTALRDAFRAETNPARRRTLARNLERWRWM
                     PVSLGADYLLVNAAAFDLGRWRGGQRVGHWKVIVGKTSSPTPVFAATVTGVNLNPWWN
                     VPSNIVRESVGGLIRRSPATARARGYVWGGGSVRQKPGPNNALGQMKLVMPNPYSVYL
                     HDTPSRDLFSRDVRAFSHGCVRVGDALGLVESLLEGVKTRPQIDALVASGASVTVPLG
                     RPVPVYIAYFTAGTRPDGTVAFYDDVYGRDRRLTALVAAGPSECSG"
     gene            9086..9694
                     /locus_tag="VFV30_00610"
     CDS             9086..9694
                     /locus_tag="VFV30_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEX4846616.1"
                     /translation="MKFNRRHFLGAMVAGTAGLVSSRSYAATLCGETPALLPQAMAAL
                     DAHAHRIAHRDVIGIVNFAAHSRLPRFQLVDVAGGQVLRTLLVAHGSGSDPAASGWTE
                     RFSNRPGSNASSKGAFVTGPTYVGKHGRSRKLIGLEPRNEAAERRAIVIHGADYVSQN
                     RALSEGRIGRSQGCFAVSRAEIGDLLALLGEGRLLYAWKDEG"
     gene            complement(9691..>11517)
                     /gene="recG"
                     /locus_tag="VFV30_00615"
     CDS             complement(9691..>11517)
                     /gene="recG"
                     /locus_tag="VFV30_00615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX4846617.1"
                     /translation="GRGPFRVLATDAPGNICAITYFGRNTGWAKKALPLGEKRWIAGR
                     LDQYGQMLQIVHPDHVAEESAAALGQLVEPVYPLSEGLTQGRLAALIHQALERVPDLP
                     EWIEPALADRMHWPRWRDSLELAHRGEHAAARDRLAYDELLANSLALLLVKNANRARV
                     GTPLVGDGALRGKLSLPFGLTGAQSRSVREIEEDMAQASPMLRLLQGDVGSGKTVVAL
                     QAMLLAVEAGAQAALLAPTEILARQHHETLTRMAAGTGVTIALLTGRDKGRARESILM
                     GLLDGSIQMVVGTHAIFQDAVAYKNLALVVIDEQHRFGVGQRLMLTQKGKRTPHCLAM
                     TATPIPRTLTLAQYGEMDVSRLDEMPPGRQPIDTRVVALERIGDVVGAIGRHLESGQQ
                     AYWVCPMVREQESEDLAAAEARYAELKARFGDSVVLVHGQLRPEAKDAAMERFASGAA
                     RLLVATTVIEVGVDVPNATLMVIEQAERFGLAQLHQLRGRVGRGEGKSTCLLLRGPTL
                     SETARERLALMRETQDGFRLAEEDLKLRGGGELLGTRQSGDTPFRVASLEQIQRLLPI
                     AHDDARLLIDRDGGLTGDRGEAARVLLYLFERDWGVQLLRGG"
BASE COUNT         1762 a         4113 c         3796 g         1846 t
ORIGIN      
        1 tcgcccaggg ctccaccggc ttgtcatagt aaccgctgaa cgggccgttg tagatccgct
       61 tcggctcgaa gaagatcacc ggatcgttgt cctcgatcgc ggcgatcagc aggcccttgg
      121 cgtcgtgggg cgtcgacggg atcacggttt tcagcccggc gacgtgggtg aacagcgcct
      181 cggggctctg gctgtgggtc tgcccgccaa agatcccgcc accaaagggc gagcgcacgg
      241 tgatcggggc gatgaattcc ccggccgagc ggtagcgcag ccgcgctgcc tcgctgacca
      301 gctgatcgag gcccggatag atatagtcgg caaactggat ttccggcacc ggccgcaggc
      361 cataggcccc catccccacc gccgcgccga tgatcccgca ttcgttgatc ggggtgtcga
      421 acacgcggtt cttgccgtac ttcttctgga ggcccgccgt ggcgcggaac accccgccga
      481 aatagccgac gtcttcaccg aacacgacca catccggatc gcgctccatc atgatgtgca
      541 gcgcctcgtt gatcgcctcg atcatgttga ggcgcttggt cggcgcttcg ctcaggctca
      601 ccgggccttc ctcgatctcg ctcatggctc tttccattcc ggccacttga tccggcgttc
      661 gcgcatggcc tgatcggcct gctcttcaag atgccagggc agctcttcgt agacgtcttc
      721 gaacatggtg tggaacggat ggtgcaggcc gtggcccagc acgccgttct tctccgcttc
      781 cttggtcgcg gccttgacct gctcggccag ctccaggtcc atcgcttcgt gctgttccac
      841 cgaccattcg cccagcgcga tcaggtggcg cttcagccgc atgatcggat cgcccagcgg
      901 ccactcctcg cgctcgtgcg cggagcggta ggcgctgggg tcgtccgaag tggaatgccc
      961 ttcggcgcgg tagctgaagt gctcgatcag ggtcgggccc ttgcccgccc gcgcccggtt
     1021 ggcggcccag cgcgccgcgg catagaccgc cagggcatcg ttgccgtcca cgcgcaggcc
     1081 ggcaatgcca tagccgatcg cgcgcgcggc aaaggtcgcc cgctcgcccc cggcgaaacc
     1141 ggaaaagctg gagatggccc actggttgtt gaccacgttg aacacaaccg gggcgttgaa
     1201 cacggtcgca aagatgaagg ccgcgtgggc atcgccttcc gcgctggagc cttcgccgat
     1261 ccaggtgctg gcgatccggg tgtcaccctt gatcgcgctg gccatggccc agccggcagc
     1321 ctgcggcatc tgcgtcgcca ggttgccgct gatcgtgaag aagttgtgtt cccggctgga
     1381 atacatgatc ggcagctgcc ggcccttcag cttgtcggcc cggttcgaat agatctggtt
     1441 gaccatctcc acgatcggat agccgcgcgc gatcaggatg ccctgctggc gatagctggg
     1501 gaacaccatg tcgtcgtccg acagggcaaa ggccgaagcg acgctggtcg cctcttcgcc
     1561 ggtgcacttc atgtaaaagc tggtcttgcc ctggcgctgg ccgcggtaca tccggtcgtc
     1621 aaaggcgcgg gtcagcgcca tggtccgcag catccggcgc agggtttcgg gatcaagctt
     1681 gggatcccag gccccgaccg cctggtgatc ctcgtccagc acccggatca gcccgtggca
     1741 cagcacatga gtctcttcgg tcgggcaggc ctcgtccggc cgcgcgaagg cgcccgccgg
     1801 gggaatatcg aggaacgaat agtcaaccgg atcgccgggg cgaaacggcg gttcggggac
     1861 gtgcaggttc agcggcggca ggttgccgcc tgcggttccg gcactctcgg gcccctgggc
     1921 catgatgcgc tctcccgctt cgaaagcgcc gcgcggcgcg tggaagcaaa ttttaggttc
     1981 gcgacataat atttcaaact ggttgcagcg tcaaccaaat ggcccgcgcg ggccgcggca
     2041 gaccttagcc gctttgcccg cgatggccag acccgttgcg cagcccctat ccggccttgc
     2101 cgtgttgcca tttgccgcca gacctgacac cttgccggca gggtctgcga aggagcgcgt
     2161 tgctgtgcgt tggaagacct gtctgcttgc cctgccgctg gtcctggccg cccccgtcct
     2221 gtccgcgcag gatgagccgc aagacgcctc tcccgaaacc gcgcaggacg atccgcgcgt
     2281 gcttgatcct gagaacgatc ccgaggtttt cgccgcagtc agccagaact tcacccgttc
     2341 ggtcaacgcg ccgtgcggct atggcgcggc gcggtgcccc gatccgatga cggtcgaaag
     2401 cgaagacacg gtcctggccg acaacctgct cccgcccgat gccaagccga tcaaacccaa
     2461 gccgccaaaa cccgaaaagc agcgcttcac gcgcgttatg caggatggca aatgggtgaa
     2521 agtgccgatc ccgaagaaga acaagacccg cgtcggcaac ggcttcatcg ccccgccggg
     2581 cagcgccccg tggatggcgc agatccagcg ccccctctac gtcaaggccg ccacggcccg
     2641 cgcgctggat tgggaagacc gccagttctg cggcggcgcg ctgatcgccc cgggctggat
     2701 cgtcaccgcc gcccattgcc tgaccgaata caagtccaac attcgcgccg ccgggtaccg
     2761 ggtgcggctg ggcaccaacg acatcaagac cgcgcagcag gcggtcagtt accggatcgt
     2821 ctcgatccat gagcacccgg attacgatgc cggcaactac agcaacgaca tcgctctggt
     2881 ccgcttcgcc gccgacgagc agaccaaccg cgatgcccgg gcctggatcc aggccatcgc
     2941 ggtggacggc gattcgccgg ggaccagccg gatcgaaggg cgcgaggcct atttctatgg
     3001 ctggggcctg accggcaacg gacgtccgtc gtccacgctg ctgttcggca agatccggct
     3061 ggagccggac agccgctgcc cgaagagcat gatcgcgctg tgcggccgcg ggatcggcgc
     3121 caagggttca acccagtgcc acggtgacag cggcggcccg ctggtgctgt tcaacggccg
     3181 caccccgatg ctggtcggtg tggtcagcca caacatcggc aagcaggcct gcggcaacaa
     3241 cgtgaaacag ggggtgttca cccgcatcgc cagctaccgc aaatggatcg agggcttcac
     3301 cggcccgctg cgccgtccgg ccagccggcg gtgagcggcc ccggggcccg gctgaccttc
     3361 gccgcgctgc tggccgtgct gggcacgggc accctgcccg cgcaggacac cccgtcccaa
     3421 ccgccgctct gcgatgacca aggtccctgc gccgatccgg ccgcactgga cagcgcctcg
     3481 ctgccgatga ccacggtcgc ggcctcgccg gacgactatg tgcccaagcc cgaacccgct
     3541 ccgtctccgg agaatcccac cggccaatcc tatgccgttg gcggcggctt tgtcctgacc
     3601 gaccataccg ccccgtggat ggcgcagatc cgccggccgg aacggatgac cgggatcacc
     3661 cagcgccgcc tgacctggtc cgaacgccac cagtgcagcg gcgcggtgat caagcccggc
     3721 tgggtcctga ccgccgccca ttgcctgacc gacacgctcg acctgcgcgg caacaccgcc
     3781 aatatccgcc agctcaatta ccgggtctgg ctgggcgtca aggacatcgg caaggaccgc
     3841 agcgggacca gctaccggat tgtcgggata taccatccgc agagctaccg ggccggaagc
     3901 tattccagcg atatcgccct ggtccagtat gcggtcgatc ccgagaccga cctcagccgg
     3961 ggtgtccgcg ccagcgcgat cgcggtggac cgcctgccgc cggacaagcg gcgcttcggc
     4021 aacgatgtcg tgcatttcta tggctgggga aagaccgagc gcgacgccgt ctccggcccg
     4081 ctgctcttcg gcaaggctgt ggttgcgccc gatgatcagt gcaaatggcc cagcatcgcg
     4141 atctgcgggt tgcagattgc caagatcggc atccccggcc ggcaatcgac ccagtgccac
     4201 ggcgacagcg gcgggccgct ggtctggttc agcaagcggc gcgtgcccct gctgatcgga
     4261 gtggtcagcc acaatgtcgg gaaagcgacc tgcgggaccc agcgcaccgc cggggtcttt
     4321 acccgggcat cggcctttgt cggctggatc gagggcgtgg caggcccgct gcccccggcg
     4381 cccccgcttt agcagcaccg gcgatccggc ccggcctgcc gcgcagcccg ggccggcaaa
     4441 cgcattacgg cttgatcggg ttgccgttgg gatcgttggt ttcggtcgcg gtggcggcgg
     4501 catcggtcgc cgtggcggcg ccggcatcgt cggaggccgc agccgaggct tcggtgctgg
     4561 cttcagtggt ggcggccgga gcctcttcct tcttctcgca cccggcgaga gccagggccg
     4621 aggccgcgac gagcaggccg gtggtcagtt tgcgcatggg ttgtcctcct gttgctagag
     4681 accaggcaac tatgacaggc ccaatcggga aagcaattcc gcaaatcgcg ggtcgctgcg
     4741 caccggatcg agcagcgcat cggcccccgc cagcagcaag ccggcatcgc gcgcagcggc
     4801 ggccttttcc agccggtcaa gcgcgcccgc gcggtcgccc cactgggcca gaacctgggc
     4861 ctgctggtag ttggaattcc ggccatagcg gctggagagc agggtgagcg cctgctgcgc
     4921 cccggccttg tccccggtcc gttggcgcac catcgcctgg ccctgaagcc ggaacagctc
     4981 aagcggctcg gcggcgaagg ctgcgtcggc atcggccagc tggccctgca ggtaaagcgc
     5041 ggtgccgatg gccgagttca cggcgctgag cttggggctg agctccagcg ccttgcgcat
     5101 ctgcacaacc gtgtcggcaa aatcgcgccg ggcataggca atgtaccccg ccgcgcggaa
     5161 agcgccgtgg ttcagcggat ccagctccag cgccgttccg atcgcctcgg ccgcttcggc
     5221 cggccggccg gtgtaggcgg tgtagagcgc gaaagtccgc agcacgtccg cgtcccctgc
     5281 ccccagttcg cgcgagcggt catagggcgc gcgcgcagcc ttgggatcga gccgcccgtt
     5341 gtagagggta tagcccagcg cggtgtgggc ctcggccagc tccggcgcgg cgcggatcgc
     5401 ggcttcggcg gattggaccg aacggtcgta gagcccgggg atctccgcgc tctggccggt
     5461 ttcgttggcg atcaccgcca ggatcttggc ccgcgcggcc agcgcccggg cgtacttgcc
     5521 atcgagggcc acggccgcgt cgaaccgctc cagcgcggcc cggtcggtcg cttcgtcctt
     5581 gctctggtcg gccagggcct tgccgcgcag gaaggcatca aacgccttgg cgttgccggt
     5641 gccgcccagc agcgccagat cgcccgagcg gccctcggcc acggccaggg cgctgatcgc
     5701 gctggcgagg gccttgctga cggcaaccgc gatctcggtc tgcaaggcca ggacatcgct
     5761 ggcctggcgg tcgagctgga cgctccagtc gacccccgcg tcgtgcccgt cgagcagttc
     5821 gaccgagacc cggatctgcc ccgcctcctg ccgcacgctg ccgcgcagga catgggccac
     5881 gcccagccgc ttggcgatgg tcaggacatc gtcgcccttg tcgccgcgaa actccgcgga
     5941 cgaggtcggc gccgcgatcc gcagcatccg gttgcgggcc aaagtggcgc gcagctcttc
     6001 cgacaggccg gccgagaaat agttctcggt cgcgccaagg ttggtaaagg ggatgacggc
     6061 aatggtgttc tcggccgcct gggccgcgcc gggtgccagc ccacgccaaa gcgcaaagcc
     6121 cccggccaga gcggccgccg cggtccccgc cccgatcagc agtccgcgcc gcgtcggccc
     6181 cttggcggtc cgcgctgccg gcgccgggtg cggctggccg gcgaagccca gcaccgcgcg
     6241 gatgaccgcc tggatctccg gcgcatcggc cttgccccgc cacttctgca gattgatcgc
     6301 ctggaactgc cggaaaccca gcggggcgtg gctgccatcg agcgacaccg gcaccaggca
     6361 gccgcgctcg cgcccgcggg tcgcctcgtc gcgcacccag tgcgaatcca cagaggtccg
     6421 ggtccacagc accaccacgg catcggcgga ttcgagcgcg gcctcggtcg tcggcaggaa
     6481 ggtctcgccg ccttccagca gcccgtccca ccagaccctg aggccggcct gttcgagaac
     6541 accgatcacc tgccgcgcgc gcttctggtc ggcccgggca tagctcagga atacgctggg
     6601 ccggctgcct gccgccccgt ctgcgtcggt gaccgaattc atcgcgctgc tgccgttccc
     6661 ttcgcgctac gcgaccccaa tggcgcgaag cttaggccga tccggcctag accgcaacgg
     6721 gcgcggcgat gtggccctgg ggggcgtagc cggtgacttc gaaatcctcg atccggtagt
     6781 cgaaaatgct ggcggggcgg cgcagaatca ccatgtttcc gccgcccgcg ggggtgcggg
     6841 ccagctgggc ttcgaccaga tcggcgtggt tgaggtacag gtgcgtatcg ccccccatcc
     6901 acaccagctc gcccggctcc aaatcgcact gctgggccag catccgggtg aacagcgccg
     6961 cgccgaacag gttgaacggc agccccagcg ccacgtcgca gctgcgttgg tagagcaggc
     7021 agctcagctt tccgccggcg acctggaact gataggtctt gtggcatggc ggcagggcca
     7081 tccggtccag ctcggccacg ttccagcctt cgacgatgtg gcggcggctg ccgggattgc
     7141 cgcgcaggct ttcgaccacc tgggcgacct ggttgatccc cggccccgga cggaacagcc
     7201 cgtcgccggc cggctgatag gtcggccaat cgacccactg cttgccgtaa accgggccga
     7261 gatcacccca ctgcgccgca aaggcggcgt cggccgcgat ccgttcggcg aaagcctcca
     7321 ccgaaatcgc atccccggtg gctttcacat agttggcgtg gggccactcg ttccagatct
     7381 tcaccccctg ctccaccagc gggcggatgt tggtgttgcc ggtcaggaac cagaggaatt
     7441 cgcgggccga ggttttccag tagatccgct tggtggtgat cagcggcatc gccccgcccg
     7501 ccaggtcaaa gcgaatctgc gcgccaaata ccgcgcgcgt gcccactccg gtgcggtcga
     7561 tccgctcatc cccctgggtc cagatgcgcc gcatcaggtc cagatactgc cattcccagt
     7621 gattatcgcg ggcggaatcg aggggaatcg cgggagaagc catgaaatga ccctagcggc
     7681 gagaatgccc gcttgccacc cgtggaatcg ctcactatag gcgcgctcct gcctcgggca
     7741 tcttcggatg tcctggatcg gtcggggaat agctcagcct ggtagagcac tgtcttcggg
     7801 aggcaggggc cggaggttcg aatcctcttt ccccgaccaa tttccttctt cgcgtccggc
     7861 ttcagccgga gcattcgctg gggccggcgg ccaccagcgc cgtcaggcgc cggtcgcgcc
     7921 catagacatc gtcatagaac gctacagtgc cgtcgggccg ggtcccggcg gtgaagtagg
     7981 cgatgtagac cggcaccggc cggcccagcg ggaccgtgac cgaagccccg ctggcgacca
     8041 gcgcgtcgat ctgcggccgg gtcttcaccc cttccagcag gctttccacc agccccagcg
     8101 catcgcccac ccgcacgcag ccgtggctga acgcgcggac atcgcggctg aacaggtccc
     8161 ggctcggggt gtcgtgcaga tagacgctgt aggggttggg catgaccagc ttcatctgcc
     8221 ccagcgcgtt gttcggcccc ggcttctgcc gcaccgatcc cccgccccag acatagccgc
     8281 gcgcccgcgc cgtcgccggg ctgcggcgga tcagcccgcc cacgctttcg cgcacgatgt
     8341 tggacggcac gttccaccag gggttgagat tgacccccgt gaccgtcgcc gcgaagaccg
     8401 gcgtgggcga cgaggtcttg ccaacaatca ctttccagtg gccgacccgc tgcccgccgc
     8461 gccagcggcc caggtcaaac gccgccgcgt tgaccagcag atagtccgct cccagcgaca
     8521 ccggcatcca gcgccagcgc tccagattgc gggccagggt ccggcggcgg gccggattgg
     8581 tctcggcccg aaaggcatcg cgcagcgcgg tgtattcggg atgctgcggc cgctggccgg
     8641 caaagaattc gtccagccgc ccggcggcca gcgcggcttg cagctgggcc tcgacgtcaa
     8701 tcgcgcgatc cgtatcggca atccgccagc ccgtccgctc ggccgccgat gccgtgccaa
     8761 gcagccgcat ccgcgccagc ttgagcgcca gcttggtcgc ggcgtcgtcg atcatcacgg
     8821 tgctgccccc ggcgatcgcc tcgtccagcg cggcgctgtc cggccgcgga aggccctcgt
     8881 ccggcgcggc ggagacccag gcgcgcaagg tggcgatcgt gctgggcgtc cacacgggtt
     8941 tgtggatcag accgtcggtt gccccggtca gcaggatcgt caacgcaagc agggaacgct
     9001 tcatccgcat aattccccct tcggcttgca atcggggaca cgatccccag attgccgcca
     9061 aacgaacttc gggaactgca tacccatgaa attcaaccgg cgccacttcc tcggcgccat
     9121 ggttgccggc accgccgggc tggtctcttc gcgcagctat gctgcgacgc tgtgcggcga
     9181 gaccccggcc ctgttgcccc aagccatggc ggcgcttgat gcccatgccc accggatcgc
     9241 gcaccgcgac gtgatcggca tcgtcaactt tgcggcccat tcgcggctgc cgcgctttca
     9301 gctggtcgac gtggccggcg ggcaggtgct gcgcaccctg ctggtcgcgc acggcagcgg
     9361 atcggacccg gccgccagcg gctggaccga gcggttttcc aaccgccccg gttcaaacgc
     9421 ctcgtccaag ggcgccttcg tgaccgggcc gacctatgtc ggcaagcacg gccgctcgcg
     9481 caagctgatc gggctggaac cccgcaacga agcggccgaa cggcgcgcga tcgtgatcca
     9541 cggggccgac tacgtcagcc agaaccgcgc cctgtccgaa ggccggatcg gccgcagcca
     9601 gggctgcttt gccgtctcgc gcgcggaaat cggcgatctg ctggccctgc tcggcgaagg
     9661 ccgcctgctc tacgcctgga aggacgaggg ctagcccccg cgcagcagct gaacgcccca
     9721 gtcgcgttcg aacaggtaga gcagcacccg cgctgcttcg ccgcggtcgc cggtcaggcc
     9781 gccatcgcgg tcgatcagca ggcgcgcgtc atcgtgggcg atcggcagca agcgctggat
     9841 ctgctcaagg ctggcaaccc ggaacggcgt gtcgcccgac tggcgggtac cgagcagttc
     9901 gcccccgccg cgcagtttca ggtcctcctc ggccagccgg aacccgtcct gcgtttcgcg
     9961 catcagcgcc agccgctcgc gcgcggtttc ggacagcgtg ggcccgcgca gcagcaggca
    10021 ggtgctcttg ccctcgcccc gccctacccg gccgcgcagc tggtgaagct gggccaggcc
    10081 gaaccgttcg gcctgctcga tcaccatcag cgtcgcgttg ggcacgtcga cgccgacctc
    10141 gatcaccgtg gtcgccacca gcagccgcgc cgcgccgctg gcaaagcgtt ccatcgcggc
    10201 gtccttcgcc tcgggccgca gctggccgtg gaccagcacg acgctgtccc cgaaccgggc
    10261 cttgagctcc gcatagcggg cctcggccgc ggccaggtcc tcgctttcct gctcgcgcac
    10321 catcgggcag acccaatagg cctgttgtcc gctttccaga tgccgcccga tcgcgccgac
    10381 cacgtcaccg atgcgttcca gcgccacgac gcgcgtgtcg atcggctggc ggccgggcgg
    10441 catttcatcc agccggctga cgtccatctc gccatactgg gcgagcgtca gggtgcgcgg
    10501 gatcggggtg gcggtcatgg ccaggcaatg cggggtgcgc ttgccctttt gcgtgagcat
    10561 cagccgctgg ccgaccccga agcggtgctg ctcgtcgatc accaccagcg cgaggttctt
    10621 gtaggcgacc gcatcctgaa agatcgcgtg ggtgccgacc accatctgga tcgaaccgtc
    10681 cagcagcccc atcaggatcg attcgcgcgc ccggcccttg tcgcgcccgg tcagcagcgc
    10741 gatcgttacc ccggttccgg ccgccatccg ggttagtgtt tcgtggtgct ggcgcgccag
    10801 gatttcggtc ggggcgagca gcgcggcctg cgcacccgct tccaccgcca gcagcatcgc
    10861 ctgcaaggcc accaccgtct tgcccgaacc gacatcgccc tgcagcagcc gcagcatcgg
    10921 gctggcctgg gccatgtcct cctcaatctc ccgcaccgaa cggctttgcg ccccggtgag
    10981 gccaaacggc aggctcagct tgccgcgcag cgcgccgtcg cccaccagcg gcgtgccgac
    11041 ccgggcgcgg ttggcgttct tgaccaacag cagcgccagg ctgttggcga gcagctcgtc
    11101 ataggccagc cggtcccgcg cggcggcgtg ttcgccgcgg tgggccagtt ccagcgagtc
    11161 gcgccagcgc ggccagtgca tccggtcggc cagcgccggc tcaatccatt ccggcaggtc
    11221 cggcacccgt tccagcgcct ggtggatcag cgcggccagc cgtccctggg tgagcccttc
    11281 ggacagcgga tagaccggct caaccagctg gccgagcgcg gcggcgcttt cctcggccac
    11341 gtggtccgga tggacgatct gcagcatttg cccgtactga tcgagccgcc cggcgatcca
    11401 gcgcttctcg cccaggggca gcgccttttt tgcccagccg gtgttccgcc cgaaataggt
    11461 gatcgcgcag atgttaccgg gcgcgtcggt ggccagcacc cggaacggcc cccgccc
//
LOCUS       DASUDV010000029         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_89301,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000029 DASUDV010000000
VERSION     DASUDV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89301"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..976
                     /gene="argB"
                     /locus_tag="VFV30_00620"
     CDS             80..976
                     /gene="argB"
                     /locus_tag="VFV30_00620"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX4846618.1"
                     /translation="MPHNHDPAMLTKAETLVEALPYLQRYAGKTFVVKYGGHAMGDPE
                     LARDFAEDVVLLKAVGINPVVVHGGGPQIGAMLKKLGVESTFVDGLRVTDKATAEVAE
                     MVLSGAINKEIVRWIAGAGGKAIGISGKDGGLVTASKVQRTSKDPGSNIEQVVDLGFV
                     GEPKAVDTSVIKTISDAGMIPVIAPIAVGEDGETYNINADTMAGAIAAAVGAARLFLL
                     TDVAGVLDKAGQLLTDLTPADIAALQTDGTISGGMIPKLETCVHAVEAGCEAAVVLDG
                     RVPHAMLLEIFTVRGAGTLIRA"
     gene            1040..1333
                     /locus_tag="VFV30_00625"
     CDS             1040..1333
                     /locus_tag="VFV30_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX4846619.1"
                     /translation="MLYALIDVIGYLVQIIIMLVILQFVLGLLMAFNVVNPHNEVFGA
                     LWRTCNQLLDPLLRPIQKIMPDTRPIDLSPMVLIIGLSILQRVLHQAAMSGLQ"
     gene            1330..2226
                     /gene="folD"
                     /locus_tag="VFV30_00630"
     CDS             1330..2226
                     /gene="folD"
                     /locus_tag="VFV30_00630"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX4846620.1"
                     /translation="MSAAVIDGKAFAATLRGRIAEYAATFEQSAGRKAGLAVVLVGED
                     PASRVYVASKGKATLECGMASFEHKLPADASEEQLLGTIAALNADPAVDGILVQLPLP
                     AHLDEQKVISTISPDKDVDGFHVTNAGRLAVGQPGFVPCTPLGCLMLLKDRLGSLSGL
                     DAVVIGRSNIVGKPMAQLLLAESCTVTIAHSRTKDLAEVVRRADIVVAAVGRPEMVKA
                     DWIKPGATVIDVGINRLPPADGESKGRLVGDVDYAGAAAVAGAITPVPGGVGPMTIAV
                     LLRNALVAAHRNAGVALADGAL"
BASE COUNT          398 a          797 c          822 g          383 t
ORIGIN      
        1 cggatcgggc gccggctgga ttcggctccg gcctaaaaag gcttcaaatc cgcgggaagg
       61 gggctaaagc gccagcatca tgccgcacaa ccacgatccc gcgatgctga ccaaggccga
      121 aacgctggtc gaggcgctgc cttatctcca gcgctatgcc ggcaagacct ttgtggtgaa
      181 gtacggcggc cacgccatgg gcgatcccga actggcgcgc gactttgccg aggacgtggt
      241 cctgctgaag gcggtcggga tcaacccggt ggtggttcac ggcggcgggc cgcagatcgg
      301 cgccatgctg aagaagctgg gggtcgaaag caccttcgtc gatggcctgc gggtaaccga
      361 caaggccacg gccgaagtgg ccgagatggt cctgtcgggc gcgatcaaca aggaaatcgt
      421 ccgctggatc gccggtgccg gcggcaaggc gatcgggatt tcgggcaagg acggcgggct
      481 ggtcacggcc agcaaggtcc agcgcacttc caaggatccc ggcagcaaca tcgaacaggt
      541 tgtcgacctc ggcttcgtgg gtgagcccaa ggcggtcgat acctcggtga tcaagaccat
      601 ttccgatgcc gggatgatcc cggtgatcgc cccgatcgcg gtgggcgagg acggggaaac
      661 ctacaacatc aacgccgata ccatggccgg ggcgatcgcc gccgccgtgg gcgcggcgcg
      721 gctgttcctg ctgaccgacg tggccggcgt gctcgacaag gcgggccagc tgctgaccga
      781 tctgaccccg gcagacatcg ccgcgctgca gaccgacggc acgatcagcg gcgggatgat
      841 ccccaagctg gaaacctgcg tccacgcggt cgaggccggc tgcgaagcgg cggtcgtgct
      901 ggatggccgg gtgccgcacg ccatgctgct ggaaatcttt accgtgcgcg gggccggcac
      961 cctgatccgg gcctgacccg ttgctccgcc cggccgcttt ccgctatcaa cccccgatca
     1021 agaacagaag gatcgccccg tgctctacgc gctgatcgat gtgataggtt acctggtcca
     1081 gatcatcatc atgctggtga tcctccagtt cgtgctgggc ctgctgatgg ccttcaacgt
     1141 ggtcaatccc cacaacgagg tgttcggggc gctgtggcgc acctgcaacc agctgctcga
     1201 tccgctgctc cggccgatcc agaagatcat gcccgatacc cggccgatcg acctgtcgcc
     1261 gatggtcctg atcatcggcc tgtcgatcct ccagcgcgtg ctgcaccagg ccgccatgtc
     1321 ggggctgcaa tgagcgccgc ggttatcgac ggcaaggctt ttgccgccac cctgcgcggg
     1381 cggatcgccg aatatgccgc cacgttcgag cagagcgccg ggcgcaaggc gggcctggcc
     1441 gtggtgctgg tgggcgaaga tccggccagc cgggtctacg tcgcctccaa gggcaaggcc
     1501 acgctggaat gcggcatggc cagcttcgaa cacaagctgc cggccgatgc cagcgaagag
     1561 cagctgctgg gcacgatcgc cgcgctcaac gccgatccgg cggtcgacgg gatcctggtc
     1621 cagctgccgc tgccggctca ccttgatgag cagaaagtga tttccacgat cagcccggac
     1681 aaggatgtcg acgggttcca cgtcaccaac gccggccggc tcgccgtggg ccagccgggc
     1741 ttcgtgccct gcacgccgct cggctgcctg atgctgctga aggaccggct tggttcgctg
     1801 tcgggcctcg atgcggtggt gatcgggcgc tccaacattg tcggcaagcc gatggcccag
     1861 ctgctgctgg cggaaagctg caccgtgacg atcgcccaca gccggaccaa ggatctggcc
     1921 gaggtcgtcc gccgcgccga tatcgtggtg gccgcggtcg gccggccgga aatggtcaag
     1981 gcggactgga tcaagcccgg cgcgaccgtg atcgacgtcg gcatcaaccg cctgcctccc
     2041 gccgatggcg agagcaaagg ccggctggtt ggcgatgtcg actatgccgg cgcagccgca
     2101 gtggccggcg cgatcacccc ggttccgggc ggggtcgggc cgatgaccat cgccgtgctg
     2161 ctgcgcaacg cgctggtcgc cgcgcaccgc aacgccggtg tggccctggc cgacggggcg
     2221 ctgtgacggt tcgactcgcc acgcttcccg cccggcgcta aacccggcga catgaaacgc
     2281 atgattctct gggggcttgc ggtctcgctt gccaccgcgc ccgccctgtc gcgcgatcgc
     2341 cgggatggcg atggccacgg caatgccaat cccagcgccg ttgtcgcggc cgaagtggcc
//
LOCUS       DASUDV010000030         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1989155,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000030 DASUDV010000000
VERSION     DASUDV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989155"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1133)
                     /locus_tag="VFV30_00635"
     CDS             complement(186..1133)
                     /locus_tag="VFV30_00635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX4846621.1"
                     /translation="MDGAAPHDSAEGAPAPAISLVLPVHNGARYLEAALDSILTQTFT
                     DFELIAVDDCSSDSTPAILARYAAADRRVRVLTNAENLRLPASLNRGFAAARGAWYSW
                     TSDDNLLRPAMLQRLWDAAQAQPGCAVIHSDYTVIDADGAPLRQVRVGRADQLLLGNG
                     IGCSFLYRAEVHHALGGFDEHLFGLEDYDFWIRAARQFRFHPLHEDLYLYRRHDGSLT
                     DRRAKQISRMANKVLRPEMDALPRGRARAEAYVSLCCRDFRILRPGLLGRAALDDPAV
                     LWRQRRAIAFWIRHSLRQRLFQLGLAAAAPAAAAVVIAE"
     gene            complement(1183..1803)
                     /locus_tag="VFV30_00640"
     CDS             complement(1183..1803)
                     /locus_tag="VFV30_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX4846622.1"
                     /translation="MTTLYRLAADAAGIAARFGARAADGDPWGGGTLAPGQFAPVLTA
                     GREFVAGPGRAGQPLRMIPRLWGVPPPPNAGDPGRGVLTVRNPDSPFWIGNLRNSEFR
                     CLVPATALMVWGRVDPVTGKRRQLWLAPTDQPLFALAGVWKDSEVPSFALLTCEANAV
                     WREAGQQAMPVILPGDPAAWGTWLRGGWDRAAALLAPYPSSRIAPV"
     gene            complement(2100..>2450)
                     /locus_tag="VFV30_00645"
     CDS             complement(2100..>2450)
                     /locus_tag="VFV30_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846623.1"
                     /translation="FQEWFGANGELITILVLISPFIIFIPLVILGIIGTRESEDVDKL
                     SLFALIAYIPAIILTYVCFNCAKYIELEIKNVFKFLAVFVLILFGSAYASKYIDPQME
                     DLLVQLPRPPMVDQ"
BASE COUNT          481 a          784 c          789 g          396 t
ORIGIN      
        1 gctcaaggtg atgggcgggg cgatgatggt cgaggcgctg atgatcctgg gctttgtcga
       61 catctacacc cagttctggc tgcgcagcct gacccacatg caccgcggct gggcgctgag
      121 caccatggcg ctgggcctgg cgctgaccgt ggtcggcagc ctgcggctgg cctgaaggct
      181 tcgccctatt cggcgatgac aaccgccgca gcggccggtg ccgccgccgc cagacccagc
      241 tggaacagcc gttgccgcag gctgtggcgg atccagaagg cgatcgcgcg gcgctggcgc
      301 cacaggacgg ccggatcgtc cagcgccgcc cgtcccagca ggccgggccg caggatccgg
      361 aaatcgcggc agcacagcga aacataggcc tcggcccgcg cccgtccgcg cggcagcgcg
      421 tccatttccg ggcgcagcac cttattggcc atccggctga tctgcttggc gcggcggtcg
      481 gtcaggctgc cgtcatggcg gcggtagagg tagagatcct cgtgcagcgg gtgaaagcgg
      541 aactgccgcg cggcgcggat ccagaaatca tagtcttcca gcccgaacag gtgttcgtcg
      601 aacccgccca gcgcgtggtg gacttcggcc cggtagagga agctgcaacc gatcccgttg
      661 cccagcagca actgatcggc ccggcccacc cgcacctggc gcagcggcgc gccgtcggcg
      721 tcgatcacgg tgtagtccga atggatcacc gcgcagccgg gctgggcctg cgcggcgtcc
      781 cacaggcgct gcagcatagc cgggcgcagc aggttgtcgt ccgaggtcca gctgtaccag
      841 gccccgcgcg cggcggcgaa gccccggttg aggctggccg gcaagcgcag gttctccgcg
      901 ttggtcagca cccgcacgcg ccgatcggca gcggcatagc gggccaggat cgccggagtg
      961 gaatcgctgg agcaatcgtc caccgcgatc agttcgaaat cggtgaaggt ctgcgtcagg
     1021 atcgaatcga gcgccgcctc gagatagcgc gcaccgttgt gaaccggcag gacaagcgag
     1081 atcgcgggcg caggcgcgcc ttctgcggaa tcgtgcggtg cggctccgtc catggccccg
     1141 gccatagcgg caaatgttga acagttggtt cccgccgcgg ggtcagaccg gggcgatccg
     1201 gctggaggga tagggcgcca gcagcgccgc cgcccggtcc cagccgccgc gcagccaggt
     1261 gccccaggcg gccggatcgc cgggcaggat caccggcatg gcctgctgcc cggcctcgcg
     1321 ccagaccgcg tttgcctcgc aggtgagcag cgcgaagctg ggcacttcgc tgtccttcca
     1381 gacgccggcc agggcaaaca gcggctggtc ggtgggggcc agccacagct gccgccgctt
     1441 gccggtgacg ggatcgaccc ggccccagac catcagcgcc gtggccggaa ccaggcagcg
     1501 aaactcgctg ttgcgcaggt tgccgatcca gaacgggctg tccggatttc gcacggtcag
     1561 caccccgcgc ccgggatcgc cggcattggg cggcggcggc acgccccaca gccgcgggat
     1621 catccgcagg ggctgcccag cgcggcccgg cccggccacg aactcgcgcc ccgcggtcag
     1681 caccggggcg aactggcccg gcgccagggt gccgccgccc cacggatcgc catcggccgc
     1741 acgcgcgccg aaacgcgcgg caatcccggc ggcatcggcg gcaaggcggt aaagcgtggt
     1801 catggctccg gcttcgctgt cgggcgggcc cgcgtcaagc gccgctccca ccaccgacga
     1861 tccgagtatc gaagtgctac atctgtccat ggccctctgc tagcggcacg gctaggcgtg
     1921 gccgaactag ccggccatct attgtaaatg cctttgcaag cctcaactgg gagcgagtga
     1981 acggctgcgc cccaaggtca agccaatcaa tcgatcagct taggcccaaa caaacgagat
     2041 gacgcatgtt aataaagagg tagtttcaga gggacatgac gaatctgcat tttaaagatt
     2101 cattgatcaa ccataggagg cctaggtaat tggactaata aatcttccat ttgaggatca
     2161 atatattttg aagcgtaagc actcccaaat aatattagaa cgaaaacagc gagaaatttg
     2221 aatacattct ttatttcaag ctcgatgtat ttcgcgcaat taaagcatac atatgtgaga
     2281 atgatggcgg gaatgtatgc tatcaaagca aataatgaca atttatccac atcttcgctc
     2341 tcgcgagtgc caataattcc caatataacc agaggtatga atattataaa tggagatatc
     2401 aaaaccaaga ttgtaataag ttcgccattt gccccaaacc attcctgaaa
//
LOCUS       DASUDV010000031         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1414865,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000031 DASUDV010000000
VERSION     DASUDV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414865"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /gene="murJ"
                     /locus_tag="VFV30_00650"
     CDS             complement(<1..1389)
                     /gene="murJ"
                     /locus_tag="VFV30_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX4846624.1"
                     /translation="MSLVKSVGTIGGLTAISRVFGFARDMLLARMLGAGLAADAFQLA
                     FTLPNTFRRLFAEGAFSVAFVPMYSRALHSEGGAEEANRFANDVLAVFVWVLLGFSAL
                     CMVAMSGIVWLLAREYASVPGKFELSVLLSRVTFPYLFFISLTAMLSGLLNARSRFGP
                     GAFAPVLLNVFLISAILIGDKLRGSSGDDTIVAGALAIGVAVSGLAQFLYLWWETRRA
                     GVRLKITAPRLTPEVKRLGALILPATFGAGIYQISQFVDTFFATSLPQGSLTLLKLAD
                     RLNQMPLGIVGIALGTAILPMLSRHIHTGNAAEAQRLQSNAFEIATLLTLPAAVALAI
                     CAPAFCTAFFVGGKFTAADGAIMAQIVVALVAGLPAYVIVKILNPGFFAREDTRTPVW
                     TALVSLAFNIAVNLYVVQRYGIVGLAGATAASASLNCLLLYGFLHARGWFHFTWKLAG
                     RIARQLIATAAMG"
     gene            complement(1427..1954)
                     /gene="secB"
                     /locus_tag="VFV30_00655"
     CDS             complement(1427..1954)
                     /gene="secB"
                     /locus_tag="VFV30_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041550025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX4846625.1"
                     /translation="MSDEGNIITDLGMTSPLPNGADTAPAVGIISQYVKDLSVENPRA
                     PDSFQWQDPPQIDVQFNIGARQINEEVAEIELKIGLESKCQAGHQFIIELSYCGLVGM
                     RNMDEASMHAFTYAEAPRILFPFARRVVADAVRDAGFPPLMLEPIDFNGIYLQQLAAQ
                     QAQGEAAGEAPAGEG"
BASE COUNT          408 a          665 c          732 g          338 t
ORIGIN      
        1 tcccatcgcc gccgtcgcga tcagctggcg ggcgatccgg ccagccagct tccaggtgaa
       61 gtggaaccag ccgcgggcat ggaggaagcc gtagagcagc aggcagttga ggctggccga
      121 agccgcggtt gccccggcca gtccgacgat accatagcgc tgcacgacat agagattgac
      181 cgcgatgttg aaggccagcg agaccagcgc ggtccacacc ggggtgcggg tatcctcgcg
      241 cgcgaaaaat ccggggttga ggatcttgac gatcacatag gcgggaagac cggcaaccag
      301 cgcgactaca atctgcgcca tgatcgcccc gtcggcggcg gtgaatttgc cgccgacgaa
      361 gaacgcggtg cagaacgccg gggcgcagat cgccagtgcc accgcggcgg gcagggtcag
      421 cagcgtggca atctcgaagg cgttggattg caggcgctgg gcttcggcgg cgtttccggt
      481 gtggatgtgg cgcgagagca tcggcaggat tgccgtgccc agcgcgatcc cgacgatccc
      541 cagcggcatc tggttgagcc ggtcggcgag cttgagcagg gtcagcgaac cctgcggcag
      601 cgaggtcgcg aagaaggtgt cgacgaactg gctgatctgg tagattcccg cgccgaaagt
      661 ggcgggcagg atcagcgcgc ccagccgttt cacctcgggc gtcagtcgcg gcgctgttat
      721 cttgaggcgg accccggcgc ggcgggtttc ccaccacagg taaaggaact gcgccaggcc
      781 ggaaaccgca accccgatcg ccagcgcgcc ggccacgatc gtatcatcgc cgctgctgcc
      841 gcgcagcttg tcgccgatca ggatcgcact gatcaggaag acgttgagca gcacgggcgc
      901 gaaggccccg ggtccgaagc gcgagcgggc gttgagcagc cccgaaagca tcgcggtcag
      961 gctgatgaag aaaaggtagg ggaaggtcac ccggctgagc agcaccgaaa gctcgaactt
     1021 gccgggaacc gatgcatatt cgcgggccag cagccagacg atccccgaca tcgccaccat
     1081 gcacagcgcg ctgaaaccaa gcagcaccca tacgaacacc gccagcacat cgttggcaaa
     1141 gcggttggct tcctccgcgc cgccttcgct gtgcagggcg cggctgtaca tcggcacgaa
     1201 ggccacgctg aaggcgcctt cggcgaacag ccggcgaaag gtgttgggca gggtgaaggc
     1261 cagctggaac gcgtcggccg cgaggccggc gcccagcatc cgggccagca gcatgtcgcg
     1321 ggcaaaaccg aagacacggc tgatcgcggt cagcccgccg atcgtgccga cgcttttgac
     1381 caggctcatg ccggcccgcc ccgcctgtgc ccccccgggc gcggcatcag ccctcgccgg
     1441 ccggagcttc gccggcggct tcgccttggg cctgctgggc ggccagctgc tgcaggtaga
     1501 tgccgttgaa atcgatcggc tcgagcatca gcggcgggaa accggcatcg cgcaccgcat
     1561 cggcaaccac gcggcgggcg aaggggaaca ggatgcgcgg cgcttcggca taggtgaagg
     1621 cgtgcatcga ggcttcgtcc atgttgcgca tgccaaccag gccgcaatag gacagctcga
     1681 tgatgaactg gtgaccggcc tggcatttgg attcgaggcc gatcttcagt tcgatctcgg
     1741 ccacttcctc gttgatctgg cgcgcaccga tgttgaactg cacgtcgatc tgcggcggat
     1801 cctgccactg gaagctgtcg ggcgcacgcg gattttcaac cgagaggtcc ttcacgtatt
     1861 gcgaaatgat gcccaccgcc ggcgcggtat cggcgccgtt gggcaggggg ctggtcatgc
     1921 caagatcggt gatgatgttg ccttcgtcgg acattgtcgt gctggctttc gcttggaaat
     1981 ggtaagaatt gggggcgcgc ctagcaccgg cgcggggcag cggcaatctt gtaacgcttc
     2041 gatcacaaaa aatgcacagg tcgggccgtc cacacgttgt tcatttgtca atgctaccta
     2101 tttggcgcta gagtgacgca aagccgcccc ttgcggcaag gac
//
LOCUS       DASUDV010000032         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_243979,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000032 DASUDV010000000
VERSION     DASUDV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243979"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1138)
                     /locus_tag="VFV30_00660"
     CDS             complement(128..1138)
                     /locus_tag="VFV30_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX4846626.1"
                     /translation="MIRTRLTERLGLDLPIMLAPMALAGGGELAAACAGAGAFGLVGG
                     GYGEREWISHEWAIATNCLANSAAAQRIGCGFITWKLDEDASALDWLLDQPLAPKAVF
                     LSFGDPRPYAARIAASGARLICQVQRLDQVPQAVEAGAAIIVAQGGAAGGHGAAAATA
                     RSTLVFVPEVADWLAAQAPDVLLLAAGGIADGRGLAASLMLGADGVLMGTRLWATREG
                     LAAQAAKEHAIEQDGDATARSAIFDVLRRKNWPIEYDFRALRNALHREWEGREDQLRA
                     DPQAARATFDAAAAAGDFTGANVTVGEAIGLIGDVPGAEDILRRIGRQAESVLHRAAD
                     WL"
     gene            complement(1135..1977)
                     /locus_tag="VFV30_00665"
     CDS             complement(1135..1977)
                     /locus_tag="VFV30_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX4846627.1"
                     /translation="MVRQRRFTQVDVFTQVPYKGNPVAVVLDGEGLSDAEMQAFANWT
                     NLSETTFVVPVTIPGADYHVRIFTPKAELPFAGHPTLGTAHAVIAAGLAEPKEGRLVQ
                     QCKIGLVELTIGESGPSFRLPRYALESLSDPEASAWLGEGVRLVGTPHAVNVGPTWLI
                     AELADVAALENLAHDPARLTRYYERTGMTGATIFAIDGERVVVRSFAPGDGIPEDPVC
                     GSGNAAVAAYRLAMGQIGDGSSYLASQGRQVGRDGWIAARIDGADVHIGGGCVTCIEG
                     TVAL"
BASE COUNT          361 a          739 c          651 g          347 t
ORIGIN      
        1 caaggttcag ggcggttttg ccgaagtcgg cgaaaacggc ctgaccgtgc tggccgaaca
       61 cgtcgaaggc tgaagcggta gcgcaatcga acaagcgaag ggcggtcctt ggggccgccc
      121 ttttcgttta gagccagtcg gcggcgcggt gcagcaccga ttccgcttgc cggccgatcc
      181 gccgcaggat gtcctcagcc cccggtacat cgccgatcag gccgatcgct tcgccaacgg
      241 tcacgttggc tccggtgaaa tcacccgcgg cagcggcagc gtcaaaggta gcgcgcgcag
      301 cctgcggatc ggcgcggagc tgatcctcgc gcccttccca ctcgcggtgc agcgcattgc
      361 gcagcgcgcg aaaatcgtat tcgatcggcc agttcttgcg tctgagcacg tcgaagatcg
      421 cactgcgggc ggtggcatcg ccatcctgct cgatagcgtg ctccttcgcg gcctgggcgg
      481 ccaacccttc gcgggtagcc cacagccggg tgcccatcaa cacgccatcg gcgccaagca
      541 tcagtgatgc cgccagcccc cgcccatcgg caattccgcc ggccgccagc agcagcacat
      601 caggcgcctg ggcggcgagc caatcggcaa cttcgggcac gaaaaccagc gtcgagcgcg
      661 cggttgccgc agccgcgccg tgcccgccgg ccgctccgcc ctgggcaacg atgatcgctg
      721 ccccggcctc gaccgcctgg ggcacctgat cgaggcgctg gacctggcag atcaaccgcg
      781 cgccgctggc cgcgatccgg gccgcatagg ggcgcggatc gccaaacgag aggaacaccg
      841 ccttgggagc caagggctgg tcgagcagcc aatcgagcgc cgaggcatcc tcatccagct
      901 tccaggtgat gaagccgcat ccgatccgct gcgccgctgc gctgttggcc aggcagttgg
      961 tcgcgatggc ccattcgtgg ctgatccatt cgcgttcgcc atagccgcca ccgaccaggc
     1021 caaaggcccc tgccccggcg caggccgcgg ccagttcgcc cccgccagcc agcgccatcg
     1081 gcgccagcat gatcggaagg tcgagaccaa gccgttcggt cagccttgtg cggatcacag
     1141 cgccaccgtg ccctcgatgc aggtcacgca gccgccgccg atgtgcacgt ccgcgccatc
     1201 gatccgcgca gcgatccagc catcgcggcc cacctgccgc ccctggctgg caagatagct
     1261 gctgccgtcg ccaatctgtc ccatcgccag gcgataggcg gccactgccg cgttgccgct
     1321 gccgcagacc ggatcttctg gaatcccatc gccgggagcg aaactgcgca ccaccacccg
     1381 ttccccgtcg atcgcgaaga tcgttgcccc ggtcatgccg gtccgctcgt aatagcgggt
     1441 caaccgggcc gggtcgtgcg ccaggttttc cagcgcggcc acatcggcca gttcggcaat
     1501 cagccaggtt ggcccgacat tgaccgcgtg gggcgtgccc accagcctta ctccttcccc
     1561 cagccatgcg ctggcttcag ggtcggacag gctttcgagc gcatagcgcg gcaggcggaa
     1621 cgagggcccg ctttcaccga tcgtcagttc gaccagcccg atcttgcatt gctgcaccag
     1681 ccggccctct ttcggttcgg ccagcccggc ggcaattacc gcatgggccg tgcccagcgt
     1741 cgggtggccg gcaaaaggca gttcggcctt aggagtgaag atccgcacgt gatagtccgc
     1801 gcccgggatg gttaccggca ccacgaaggt ggtttcgctg aggtttgtcc agttggcgaa
     1861 ggcctgcatt tcggcatcgc tcagcccttc gccatcgagc acgacggcaa ccggattgcc
     1921 cttgtagggc acctgcgtga acacatcgac ctgggtgaag cgccgctgac ggaccatcga
     1981 atcaaactcc tgcttgcatc gcctcgatgt atatcgtaaa attgcgatat tcaaatatta
     2041 cgttcgatcg aaggattgga acagcttgct cgaccgtcag ctcaaggcca ttgccaac
//
LOCUS       DASUDV010000033         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2188176,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000033 DASUDV010000000
VERSION     DASUDV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188176"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFV30_00670"
     CDS             <1..181
                     /locus_tag="VFV30_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX4846628.1"
                     /translation="VSAKIMGSGNVSVKGQAKCTVSAMGSGTLSCESGPATVVNDDDK
                     AAPAAPEAPEAPKVP"
     gene            complement(186..1127)
                     /locus_tag="VFV30_00675"
     CDS             complement(186..1127)
                     /locus_tag="VFV30_00675"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX4846629.1"
                     /translation="MSDKVKIAPADPAGIAAAAAILRDGGLVAVPTETVYGLAARADS
                     ADAVAAIYRAKGRPDFNPLIVHVPDRAGAETFAVLDDRARRLAAAFWPGALTLVVPLR
                     EGARIASAVTAGLSTIALRCPAHPVMQALLKAAGLPLAAPSANRSGAISPTRAGHVAA
                     SLGGRVDLILDGGSCAAGLESTIVALREDGSWQLLRPGPIAPERIAEVLGAAPAPIAS
                     QRIEAPGQLASHYAPGKPMRLSVMEARPDEFHIGFGPVPGHSSLSASADLAEAGARLY
                     DCLHQAAGSAYPKIAVAPVPDSGIGAAINDRLRRAAA"
     gene            1181..2890
                     /locus_tag="VFV30_00680"
     CDS             1181..2890
                     /locus_tag="VFV30_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4846630.1"
                     /translation="MDYAAPTADQVLAIRVNAGIEELASHPRFAAASPDMVEAIVEGV
                     GQFAAGEFAPLNRKGDLEGARLANGTVSLPEGFEAAYRAFVEQGWNSISGPESHGGQG
                     LPFTLATNVIENLGSANMAFTLLPLLTVGAIEALHHHGSAAQQALYLPRLISGEWSGT
                     MNLTEPQAGSDVGALRTTATPRGDGTWLVKGTKIFITWGEHELARNIVHLVLARTPGA
                     PAGSRGISLFVVPKYLVGPDGSLGSRNDLRCVSLEHKLGIMASPTCVMSFGDNDECIG
                     ELVGHENEGLKAMFTMMNSARINVGNQGVQIAERALQQAKAYARDRVQSARAGSADKH
                     PVAIVEHPDVRRMLLRMRALTEGARALLYYTAGQVDRGALGVEGAQSRADCLVPLIKA
                     WGTDVGCEVASLGVQIHGGMGFIEETGAAQHYRDARIAPIYEGTNGIQAADLVTRKLG
                     YEGGAVLIALFDEIVRDCADAPELAALAGECGAIARWMAGEASLDDKLAGSVPFCTMS
                     AVAVAGWQLQRQARDGASGVAKQVVARYFAEHIAPEARGLKAAATAGAGLLYALDAET
                     LSA"
     gene            2887..3702
                     /locus_tag="VFV30_00685"
     CDS             2887..3702
                     /locus_tag="VFV30_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4846631.1"
                     /translation="MSDELARIAAALERLSPGEAPKPDWLSAPAYVWDGTARAVEKIL
                     APQLALLKGIDAQKEAVRANVERLARGAAAHDMLLWGARGMGKSALLRSAVLAAQDHA
                     PGALALIQIAPDALASLPALFGALRGVERRFLVFIDDLGFEDGDSTGPRALRSWLEGG
                     VEARPANVRIAVTSNRRAIVARHLSEQDDPINPRDAVDDRLALADRFGLSIGFNNCSQ
                     DDYLAIISGYCSELGLTFEEGEALEWSKRRGARSGRVAWQFVTELAGRAGKPL"
     gene            complement(3712..>3898)
                     /locus_tag="VFV30_00690"
     CDS             complement(3712..>3898)
                     /locus_tag="VFV30_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX4846632.1"
                     /translation="LLVSDDTAGIIWRVIAPDAKPAPPVRPVITGHMPPQRTLNSDPE
                     ARFRAKIKADDETIRQQ"
BASE COUNT          649 a         1296 c         1366 g          587 t
ORIGIN      
        1 cgtttcggcc aagatcatgg gttcgggcaa cgtttcggtg aagggccagg ccaagtgcac
       61 cgtaagcgcg atgggttcgg gcacgctgag ctgcgaatcg ggaccggcaa ccgtggtcaa
      121 tgacgacgac aaggccgctc ccgcagcgcc agaagctccc gaggcgccca aggtaccctg
      181 attgatcagg cagcagcgcg gcgcaggcgg tcgttgatcg ccgcgccgat gccgctgtcg
      241 ggaacgggcg cgaccgcgat cttcgggtag gccgaaccgg ccgcctgatg caggcagtcg
      301 tagagccggg ccccggcctc ggctaggtcg gcgcttgccg aaaggctgct gtgccccgga
      361 accgggccaa agccgatgtg aaactcgtcg ggcctggctt ccatcacact cagccgcatc
      421 ggctttcccg gtgcatagtg gcttgccagc tggcccggcg cctcgatccg ctgcgaggcg
      481 atcggcgccg gcgccgcgcc cagcacctcg gcgatccgtt ctggcgcgat cgggcccggg
      541 cgcagcagct gccacgaacc atcctcgcgc aaggcaacga tcgtgctttc cagacccgcc
      601 gcgcaggatc cgccatcaag aatgagatcg acacgcccgc ccagcgaggc ggcgacgtgc
      661 ccggcccggg tcgggctgat cgcaccgctg cggttggccg aaggcgccgc gagcggcagt
      721 cccgctgcct tgagaagtgc ctgcataacc ggatgggccg ggcagcgcag ggcgatggtc
      781 gataggccgg cagtcaccgc cgaagcgatt cgcgcgccct cgcgcagcgg cacgaccagg
      841 gtcagcgcgc cgggccagaa cgccgcggcc agccggcggg cgcgatcgtc gagcacggcg
      901 aaggtttcgg caccagcccg atcgggcaca tggacgatca gcggattgaa atcggggcgc
      961 cccttggccc ggtagattgc cgctacggca tcggcactgt cagcccttgc ggccaggcca
     1021 tagaccgtct cggtcggcac cgcgaccagc ccgccatccc gcaggatcgc ggcggcggca
     1081 gcaattccgg ccggatcggc cggagctatc ttgaccttgt cggacatgcc cctcccgcta
     1141 taggcacaag ggctcgaagc caaggaaaag cgagatgccg atggactatg ccgcaccaac
     1201 cgccgaccag gttctggcga tccgggtcaa cgccgggatc gaagaactgg ccagccatcc
     1261 ccgctttgcc gccgccagcc cggatatggt cgaagcaatc gtcgaggggg tcggccagtt
     1321 tgccgccggc gaattcgcgc cgctaaaccg caagggcgat ctcgaaggcg ccaggctcgc
     1381 caacggcact gtcagcctgc ccgaagggtt cgaagcagct taccgggcat ttgtcgaaca
     1441 gggctggaac tcgatttccg gacccgaatc gcacggcggg caggggctgc cctttaccct
     1501 tgccaccaac gtgatcgaga acctgggcag cgccaacatg gctttcaccc tgctgccgct
     1561 gctgaccgtc ggcgcgatcg aggcgctcca tcaccacggc agcgcggccc agcaggcgct
     1621 ctacctgccc cggctgatca gcggcgaatg gtcgggaacg atgaacctga ccgagcccca
     1681 ggccggttcg gatgtgggcg cgctgcgcac caccgcaacg ccgcgcggtg atggcacctg
     1741 gctggtcaag gggaccaaga tcttcatcac ctggggtgaa cacgaactcg cccgcaacat
     1801 cgtccacctg gtcctggcgc gcaccccggg tgcgccggcc ggcagccgcg ggatcagcct
     1861 gttcgtggta cccaaatacc ttgtcgggcc ggacggatcg ctcggcagcc gcaacgatct
     1921 gcgctgcgtc agcctggaac acaagctggg gatcatggcc agcccgacct gcgtcatgag
     1981 ctttggcgac aacgacgaat gcatcggcga actggtgggg cacgagaacg aaggcctcaa
     2041 ggccatgttc acgatgatga actcggcccg gatcaatgtt ggcaaccagg gcgtgcagat
     2101 cgccgagcgc gcgctgcagc aggccaaggc ctatgcccgc gaccgggtcc agtcggcccg
     2161 cgccggatcg gccgacaagc acccggtggc gatcgtcgag cacccggatg tgcggcgcat
     2221 gctgctcagg atgcgggcgt tgaccgaagg cgcgcgcgcg ctgctctact ataccgcggg
     2281 gcaagtcgat cgcggcgcat tgggggtcga gggtgcccag tcccgcgccg attgcctggt
     2341 gccgctgatc aaggcctggg gcaccgatgt cggctgcgag gtcgccagcc ttggcgtgca
     2401 gatccacggc gggatgggct tcatcgagga aaccggcgcg gcccagcact atcgcgatgc
     2461 ccggatcgcc ccgatctatg aagggaccaa cgggatccag gccgccgatc tggtaacccg
     2521 caagctgggt tatgaaggcg gcgcggtgct gatcgcgctg ttcgacgaga tcgtgcgcga
     2581 ttgcgccgat gcacccgagc tggccgcgct ggcgggcgaa tgcggcgcca ttgcgcgctg
     2641 gatggcgggc gaggccagcc ttgacgacaa gctggccgga tcggtcccgt tctgcacgat
     2701 gagcgcggtt gccgtggccg gctggcagct gcaacgccag gcccgcgatg gggcgagcgg
     2761 cgtggccaag caggttgtcg cgcgctattt tgccgagcac atcgctcccg aagcgcgcgg
     2821 gctcaaggcc gcggcaacgg ccggcgcggg gctgctctat gcgctcgatg ccgaaacgct
     2881 gagcgcatga gcgacgagct ggcccggatt gccgccgcgc tcgaacggct gagcccggga
     2941 gaggcgccaa agcccgactg gctgagtgca cccgcctatg tctgggatgg cacggcgcgc
     3001 gcggtcgaga agatcctcgc cccgcagctt gccctgctca agggcatcga tgcacagaag
     3061 gaagcggtgc gggccaatgt cgagcgactc gcccgcggtg cggcggcgca tgacatgctg
     3121 ctgtggggcg cgcgcggcat gggcaagtca gcgctgctgc gctcggcggt gctggcagca
     3181 caggatcatg cgcccggcgc actggcgctg atccagattg cgcccgatgc gctggccagc
     3241 ctgccggcgc tgttcggggc gctgcgcggg gtggagcggc gattcctggt ctttatcgac
     3301 gatctaggct tcgaggatgg tgacagcacc ggcccgcgcg cgctgcgttc ctggctggaa
     3361 ggcggggtcg aggcacgccc ggccaatgtc cgcatcgccg tgacgagcaa tcgccgcgcg
     3421 atcgttgccc gccatctgtc cgagcaggac gatccgatca acccgcgcga tgcagtggac
     3481 gaccggttgg cgctggccga ccgtttcggc ctgtcgatcg ggttcaacaa ttgcagccag
     3541 gacgattacc tggcgatcat ttccggctat tgcagcgaac tgggtctgac cttcgaagag
     3601 ggagaggcgc tcgaatggtc caagcggcgc ggcgcccgtt cgggccgcgt cgcctggcaa
     3661 ttcgttaccg agcttgccgg cagggcgggc aagccgctct agggtcagga cctattgctg
     3721 acggatcgtc tcgtcgtcag ccttgatctt ggcacggaac cgggcttcgg gatcgctgtt
     3781 aagcgtgcgc tgcggcggca tgtgcccggt gatcaccggg cgcaccggcg gcgccggctt
     3841 ggcatcgggc gcgatcaccc gccagatgat cccggcggta tcgtcgctca ccagcagc
//
LOCUS       DASUDV010000034         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_354556,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000034 DASUDV010000000
VERSION     DASUDV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354556"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VFV30_00695"
     CDS             complement(<1..106)
                     /locus_tag="VFV30_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX4846633.1"
                     /translation="MLGRTANGVFWMARYLERAENTARLLDAGSRMALT"
     gene            complement(111..1538)
                     /locus_tag="VFV30_00700"
     CDS             complement(111..1538)
                     /locus_tag="VFV30_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX4846634.1"
                     /translation="MGQSTAAFDEMLKADGGVRAAYADYREWFDAQDPALLRRKDGEA
                     ENFFRRTGITFNVYGDDAGEERLIPFDMVPRIVTPSEWRKLSRGIEQRVRALNAFLHD
                     IYHRQEIVRAGRLPQRLIAENAAFLPQMVGFVPPGGVYTHIVGVDLVRTGPDEFMVLE
                     DNTRTPSGVSYMLENRETMMAMFPELFTKVKVAPVQDYPRRLARSLAACAPTAAGDRP
                     TVAVLTPGIYNSAYFEHAFLADQMGAELVEGSDLRVVDGRVQMRTTRGYQPIDVLYRR
                     VDDEFLDPLSFNPDSVLGVPGIMDVYRSGGITIANAPGTGIADDKAIYSFMPEIVEFY
                     TGEKPLLSNVPTWRCSEKDSLAYVLDNLAELVVKEVHGSGGYGMLIGPTSSKKEIAAF
                     AQKLRAKPANYIAQPTLSLSTVPIFTKAGLAPRHVDLRPFVLVSPQGIDITPGGLTRV
                     ALKKGSLVVNSSQGGGTKDTWVLED"
     gene            complement(1611..2534)
                     /locus_tag="VFV30_00705"
     CDS             complement(1611..2534)
                     /locus_tag="VFV30_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846635.1"
                     /translation="MGKPDRIDRLAQALVLIAALIALGNGFKMLIDPFGWYDLVGTVK
                     ATGPANAHFIRDIGLAFLLSAALLGYGGSNLALRWGAALAGAGWLLAHGLLHIWEVLA
                     GICAASIFWQEAPGTLGPPLLALAGIGLMLARQRIAPFPLPGWLFVRTMDQIAPGETE
                     YVHRVAALPGGALEKLQHFMPASSHRHAAPADLFAMARIGATRAEDCGPCTLIAARWA
                     LHDGVPRELVQAALDGALPEGDQADAYAFGRSVALCQPEAADLGAVLAARHGAAVRDE
                     LALTVATIRVYPALKRGLGIAQSCSVVPLKL"
BASE COUNT          475 a          921 c          883 g          438 t
ORIGIN      
        1 gggtcagcgc catgcgcgag cccgcatcga gcaggcgcgc agtgttctcc gcccgctcaa
       61 ggtagcgggc catccagaac accccgttgg cggttctgcc cagcacgctg ctagtcctcc
      121 aagacccagg tgtccttggt cccgccgcct tgcgaggagt tgacgaccag gctgcccttc
      181 ttgagcgcga cgcgggtcag gccgccgggc gtgatatcga tcccttgcgg cgaaaccagc
      241 acgaacggcc gcaggtcgac atggcgcggc gccagcccgg ccttggtgaa gatcggcacg
      301 gtcgacagcg aaagcgtcgg ctgcgcgata tagttagccg gcttggcccg cagcttctgg
      361 gcaaacgcgg cgatttcctt tttcgaggaa gtcggcccga tcagcatccc gtagccgccc
      421 gacccgtgaa cttccttcac caccagttcg gcaaggttat cgagcacgta ggccaggctg
      481 tccttctcgc tgcagcgcca ggtgggcacg ttcgaaagca gcggcttttc gccggtatag
      541 aactcgacga tttcgggcat gaacgaatag attgccttgt cgtcggcaat cccggtgccg
      601 ggcgcattgg cgatggtgat cccgccagag cggtagacat ccatgatgcc cggcacgccc
      661 agaacgctgt cgggattgaa cgagagcgga tcgaggaact cgtcatcgac ccggcgatag
      721 agcacgtcga tcggctgata gccgcgcgtg gtgcgcatct gcacccgccc atcgacaacg
      781 cgcagatcgc tgccctcgac cagctcggcg cccatctggt cggcaaggaa ggcgtgttcg
      841 aaataggccg aattgtagat ccccggcgtc agcacggcga cagtcggccg gtctcccgct
      901 gccgttggtg cacaggcggc caggctgcgg gcgaggcgac gcggatagtc ttgcacaggc
      961 gccaccttca ccttggtgaa gagctcgggg aacatcgcca tcatcgtttc gcggttctcg
     1021 agcatgtaag atacgcccga tggcgtgcgg gtgttgtcct cgagcaccat gaactcgtct
     1081 ggcccggtgc ggaccaggtc gaccccgacg atatgggtat agaccccgcc cggcggcacg
     1141 aagccgacca tctgcggcag gaaggcggcg ttctcggcaa tcagccgctg cggcagccgc
     1201 ccggcgcgga cgatctcctg gcggtggtag atgtcgtgca ggaaggcatt gagcgcccgc
     1261 acccgctgct cgatcccgcg gctcagcttg cgccactcgc tgggggtaac gatccgcggc
     1321 accatgtcga aggggatcag ccgctcttcc ccggcatcgt cgccatagac gttgaaagtg
     1381 atgccggtgc ggcggaagaa gttctccgcc tcgccatcct tgcggcgcag cagggccgga
     1441 tcctgggcat cgaaccattc gcggtaatcg gcataggcgg cgcgcacgcc cccatcggcc
     1501 ttgagcattt catcaaacgc tgcggtgctt tgccccatgg gctgcaatga ctgcccgatg
     1561 cgcagacgga gtcaagcgcg aatgctgcga gtgcgaaacg ggctgccccg ctacagcttt
     1621 aatggcacca ccgagcagct ctgcgcgata cccagcccgc gcttcagcgc cggatagacc
     1681 cggatcgtgg cgacggtcag cgccagctcg tcgcgcacag ccgccccgtg gcgcgcggcc
     1741 aagaccgcgc ccaggtccgc tgcctctggc tggcacagtg cgacggagcg gccaaaggca
     1801 taggcatctg cctggtcgcc ttcaggcagc gcgccatcga gcgccgcctg aaccagttca
     1861 cgcggcacgc cgtcatgcag cgcccagcgc gcggcgatca gcgtgcaggg cccgcaatcc
     1921 tcggcccggg tcgcgccgat ccgggccatc gcgaacagat cggccggcgc agcgtggcgg
     1981 tggctggagg cgggcatgaa atgctggagc ttttccagcg ccccgcccgg cagcgcggcc
     2041 actcggtgga catattcggt ctcacccggg gcgatctggt ccatggtccg cacgaacagc
     2101 caccccggca gcgggaacgg cgcgatccgc tggcgggcca gcatcagacc gatcccggcc
     2161 agcgccagca gcggcgggcc gagcgtgccg ggcgcctcct gccagaagat gctcgcagcg
     2221 cagatcccgg ccagcacttc ccagatgtgc agcaggccat gcgctagcag ccagcctgct
     2281 ccagccagcg ccgcacccca gcgcagcgcc aggttgctgc cgccatagcc cagcagcgcc
     2341 gcgctcagca ggaaggccaa gccgatatcg cggatgaagt gcgcattggc tggcccggtc
     2401 gccttgaccg tgccgaccag atcataccag ccgaacggat cgatcagcat cttgaaccca
     2461 ttgcccagcg cgatcagcgc tgcgatcagg accagggctt gcgccaggcg atcgatccgg
     2521 tcaggcttgc ccatggtcat gcttcccttt gcgactcaat tcggatattt catatccata
     2581 tttaatggag ccggcaagcc atgcggatga accagggggt cgaatgggcg atacacgcct
     2641 gcgcgctgct tgccccgctg tggccggaca agggcctgag cctggccgcc ttggccgagt
     2701 ttcacggcgt gcccggc
//
LOCUS       DASUDV010000035         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2121848,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000035 DASUDV010000000
VERSION     DASUDV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121848"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFV30_00710"
     CDS             complement(<1..947)
                     /locus_tag="VFV30_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4846636.1"
                     /translation="MSFRNISAWCIRNPVVPIVLFLGLMLAGMITFSRMKVQNDPDIE
                     FPLVVVSISQPGAAPTEIENQITQKIEASLQSLQGVQSISSTAREGGSETQIEFEIGT
                     DVIEALNDVKNAVDQTRGSLPDGILEPQVFKVEISNEEIGFFSVMADDMTIEQLSWFV
                     DDTVTKNLLSVPGLAQVDRGGGVTREIRVILDPGKMQANGVSAGQINQVLRQLNLNAA
                     GGRSEIGGSRQSVRVIGNAATAFELSQTDIPLGGGRTIKLADVAKVSDSFSEITSLGK
                     TNGKSAVTFSIARARGESDVAVYDAAVARLEKIESEVPGV"
     gene            complement(973..2157)
                     /locus_tag="VFV30_00715"
     CDS             complement(973..2157)
                     /locus_tag="VFV30_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4846637.1"
                     /translation="MNYESSIDANRGPADNPLLGTDDEDRSSRRRLIYIALAVLLTMI
                     AVAYYMNRTAGGDAKAGDKKDQVPVVTVVVPGKTTVEGIISANGTLAARRELPVGVPG
                     EGGQVVAVLVEPGQWVRAGQVLAVIDRSVQSQQQSSQSAQIEVARANARLAQANLERA
                     LKLVDKGFVSKADIDRLTATRDSAEAQVRVASAQLGVLNAQVRRLNIVAPAAGLVLER
                     RVEPGQVVSGGSGVLFRLAKGGEMELRALLSEADLASLSPGVAADVTPVGSPRAFTGQ
                     VWQIAPVIDPVSRQGVARIALAYAPELRPGGFASVAIKSGTMVAPVLPESAVLSDSQG
                     TFVYVVRADNKVERRAIKQGRVTAGGIVIAEGLSGNERIVLRAGGFLQPGETIKPVSP
                     KS"
     gene            complement(2441..2740)
                     /locus_tag="VFV30_00720"
     CDS             complement(2441..2740)
                     /locus_tag="VFV30_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX4846638.1"
                     /translation="MIENQKIRPAMVIGPLGEPLTLESLPKPSTTRWVVRRKAEVVAA
                     VNGGLLTIDEVCERYGLSLEEFASWQRAVDRSGMQGLRVTRIQHYRDLYERQLKY"
     gene            complement(2740..3045)
                     /locus_tag="VFV30_00725"
     CDS             complement(2740..3045)
                     /locus_tag="VFV30_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846639.1"
                     /translation="MELGNDLLEALRQRVSPQSGAPEWAGLHARLSAAHAAARAFAQG
                     SGLVPARAGDFTLWQAHAGSNFNAERPVNPADLADGKSAEGIEHDTAGGAIAGGRGQ"
     gene            3200..4354
                     /gene="mnmA"
                     /locus_tag="VFV30_00730"
     CDS             3200..4354
                     /gene="mnmA"
                     /locus_tag="VFV30_00730"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX4846640.1"
                     /translation="MPTLALPDPASLFQLDAPLAGKRIVVAMSGGVDSSVVAALAARS
                     GAETIGVTLQLYDSGPASGRKGACCAGDDIRDARSVADRLGIAHYVIDHASQFREEVV
                     ERFADDYLAGRTPIPCIRCNMGPKFTDLLAMARDLGADCLATGHYVRRVEGAAGAELH
                     RALDPARDQSYFLFATTEEQLDYLRFPLGGLPKSQVRAIAEAEGLRVAAKPDSQDICF
                     VPDGDYARIVRGLRPEGGEPGEIVDAASGAVLGRHKGVIHYTVGQRRGLEIGGQAEPL
                     YVTGIDAVTRQVLVGPRRMLAVSAAHLVETNRIGPLDDLPLVAKVRSLAKPVPVVLDG
                     PLGDGAPTTIRFINPEYGVAPGQAAVLYAGERVVGGGWIETTEPAVSQSL"
     gene            complement(4349..5149)
                     /locus_tag="VFV30_00735"
     CDS             complement(4349..5149)
                     /locus_tag="VFV30_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX4846641.1"
                     /translation="MSVLLARHYDERPWGVDELPAPFVTPQGKRIGEVWFIPPAALDQ
                     LLVKYIFTSAALSVQVHPSNAQARAQGLSSRGKEECWLVIGAEAGARLGIGFNRALDQ
                     EAMRAAALDGSIEQLLTWHEVEAGDFFYIPAGTVHAIGPGVRLIEVQQNSDITYRLYD
                     YGRPRELHLDAGIAVARGEPYPPALRQRLSERGEIVLVDGPHFRLARLDGPASADLAA
                     RFAGPVLAIPIDCAARVGDLAVQPGQCALAPSLAELAADGLCLIAQPL"
     gene            complement(5146..>5438)
                     /locus_tag="VFV30_00740"
     CDS             complement(5146..>5438)
                     /locus_tag="VFV30_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX4846642.1"
                     /translation="MENTARAAMVPAAMGWSDIGNWDALYAAREHDRAGNCVRGSAEL
                     VECRNVMVESDGPRVSVIGLEDVVIVVDGNEVLVTTRAGAQAVGKLDGAANQ"
BASE COUNT          857 a         1897 c         1769 g          915 t
ORIGIN      
        1 acccccggca cttccgattc gatcttttcc agccgggcga ccgccgcgtc atagaccgcg
       61 acgtcggatt cgccgcgggc gcgggcgatg ctgaaggtca ccgccgattt gccgttggtc
      121 ttgccgagcg aggtgatctc gctgaagctg tcgctgacct tggccacgtc ggccagcttg
      181 atcgtgcgcc cgccgcccag cggaatgtcg gtctgcgaga gctcaaaggc cgtcgccgcg
      241 ttgccgatca cccgaaccga ctggcgcgac ccgccgattt ccgaacggcc gccggcggcg
      301 ttgaggttga gctggcgcag gacctggttg atctgtccgg ccgacacgcc gttggcctgc
      361 atcttgcccg gatcgaggat cacgcggatc tcgcgggtca ccccgccgcc gcgatcgacc
      421 tgggccaggc ccggcacgct cagcaggttc ttggtcacgg tatcgtcgac gaaccacgac
      481 agctgctcga tcgtcatgtc gtccgccatg accgagaaga agccgatttc ctcgttggag
      541 atctcgacct tgaagacctg cggctcgagg atgccgtccg gcaggctgcc acgggtctgg
      601 tcgaccgcgt tcttgacgtc gttcagcgcc tcgatgacat cggtgccgat ctcgaattcg
      661 atctgggttt cgctgccgcc ttcgcgcgcg gtcgagctga tgctttgcac cccctgcagc
      721 gattgcagcg aggcttcgat cttctgggta atctggttct cgatctcggt cggggcggcg
      781 cccggctggc tgatcgagac caccaccagc ggaaattcga tatcgggatc gttctgcacc
      841 ttcatgcgcg agaaggtgat catcccggcc agcatcaggc cgaggaacag cacgatcggc
      901 acaaccgggt tgcggatgca ccaggcagat atgttgcgga agctcatgat cggcccttgc
      961 gcgcttgctg catcacgact tgggcgaaac gggtttgatc gtctcgcccg gctggaggaa
     1021 cccgccggcg cgcagcacga tccgctcgtt tccggacagg ccttcggcaa tgacaatgcc
     1081 gccggcggtc acccggccct gcttgatcgc ccggcgctcc accttgttgt ccgcacggac
     1141 cacgtagacg aaagtgccct ggctgtcgga caggaccgcc gattccggca gaaccggagc
     1201 gaccatcgtg cccgacttga tcgccaccga ggcgaagccg cccggacgca gttcgggagc
     1261 ataggccagc gcgatccggg ccacgccctg gcggctgacc ggatcgatca ccggagcaat
     1321 ctgccagacc tggccggtga aggccctggg cgagccgacc ggcgtcacgt cggccgcaac
     1381 cccggggcta agcgaggcca gatcggcttc gctgagcagg gcccgcagct ccatctcgcc
     1441 gcccttggcg agccggaaca gcacgcccga gccgccactg acgacctggc cgggctcaac
     1501 ccggcgttcc agcaccaggc cggcggccgg ggccacgatg ttgaggcgcc gcacctgggc
     1561 gttgagcacg cccagctggg cgctggcgac gcggacctgg gcttcggccg aatcgcgagt
     1621 cgcggtcagc cggtcgatat cggccttgga cacgaacccc ttgtcgacca gcttgagcgc
     1681 gcgctccaga ttggcctgcg ccagccgggc gttggcccgg gcaacctcga tctgcgcgga
     1741 ctggctggac tgctgctgcg actggaccga acggtcgatc accgccagca cctggccggc
     1801 gcggacccac tggccgggtt cgaccagcac ggcgacaact tgcccgcctt cacccggcac
     1861 gccgaccggc agctcgcggc gcgcggccag ggtgccgttg gcgctgatga tgccttcgac
     1921 cgtggtcttg cccggcacca ccacggtgac caccggaacc tggtccttct tgtccccggc
     1981 cttggcgtcg ccgccggcgg tgcggttcat gtaataggcg accgcgatca tcgtgagcag
     2041 caccgcgagc gcgatgtaga tcagccgccg gcgcgagctg cggtcttcgt cgtccgtgcc
     2101 cagcaacgga ttgtcggccg ggccgcggtt tgcgtcgatg ctcgattcgt aattcatggc
     2161 cgcttcgctt tcgcccctgt tccggaccca agcctcccgg tcggagaggc gggtcggtgc
     2221 agactgtgtt actatgctaa atcacctgcc gcaagccctg ttgccgggcg gcggcgggaa
     2281 gaccgcgtca ctctgcgcca taggccgctg cgcccggcag ggcaatgcgg ggctcggcaa
     2341 accgccgagc cggctcggcc aacggcgttg gcccatgcaa gaacggccgg agcgagggtg
     2401 ctcgctccgg ccgtccgggt tcatcaatcc gctgggcgga tcagtatttc agctggcgct
     2461 cgtagagatc gcggtagtgc tggatccgcg tcacccgcag gccctgcatc cccgaccggt
     2521 ccaccgcgcg ttgccacgag gcgaactctt ccagcgacag gccatagcgc tcgcagacct
     2581 cgtcgatggt cagcaggccg ccgttcaccg ccgcaaccac ttcggccttg cggcgcacca
     2641 cccagcgggt ggtcgaaggc ttgggcagcg actcaagcgt caagggctcg ccaagcgggc
     2701 cgatcaccat cgcgggtcga attttctggt tttcgatcat cattgtcctc gcccaccggc
     2761 aatcgcacca ccggcggtgt cgtgttcaat gccctcggca cttttgccat ctgctaaatc
     2821 agcagggtta acgggccgtt cggcattgaa attggatccg gcgtgcgcct gccagagcgt
     2881 gaaatcaccc gctcgggccg ggaccaggcc cgaaccctgc gcaaaagcgc gcgccgccgc
     2941 gtgggccgca ctgagtcgcg cgtgcagccc cgcccattcg ggcgcgccag actgcgggga
     3001 aacccgctgc ctcagcgctt caagaaggtc atttcccaat tccatgggag ccgctctaat
     3061 gcggtaaggt caagatcggg taaaacccgg ctttaccccc tgttgagaat gcttaccgaa
     3121 ttgcaccgcc cgcctcaggg ccccggcgca gggtgcgctc tttgccttgc gcggcgctcc
     3181 ggtgtaaggc gcgcggacca tgccgaccct tgccctgccc gatcccgcct cgctgtttca
     3241 gctcgatgcc ccgctggccg gcaagcggat cgttgtggcc atgtccggcg gagtcgacag
     3301 ctcggtggtg gccgcgctgg ccgcgcgcag cggggccgaa acgattggcg tgaccctgca
     3361 gctttacgat tccggtcccg ccagcgggcg caagggcgcc tgctgcgccg gcgacgacat
     3421 ccgcgatgcc cgcagcgtgg ccgaccggct gggcatcgcc cactatgtca tcgaccacgc
     3481 cagccagttc cgcgaagagg tggttgagcg ttttgcggac gattacctgg ccgggcgcac
     3541 cccgattccc tgcatccgct gcaacatggg ccccaagttc accgatctgc ttgccatggc
     3601 ccgcgatctg ggcgccgatt gtctggccac cgggcactat gtgcgccggg ttgaaggtgc
     3661 agccggggcc gagctgcacc gcgcgctcga tccggcgcgc gaccagtcct atttcctgtt
     3721 cgccacgacc gaggagcagc tcgactacct gcgctttccc cttggcggcc tgcccaagag
     3781 ccaggtccgc gcgatcgccg aggccgaggg cctgcgcgtt gccgccaagc ccgacagcca
     3841 ggacatctgc ttcgttcctg acggcgacta tgcccggatc gtgcgcggcc tgcgccccga
     3901 gggcggcgag ccgggagaga tcgtcgatgc cgccagcggc gcggtgctgg gccggcacaa
     3961 gggcgtgatc cactataccg tgggccagcg ccgcggcctc gagatcggcg gccaggccga
     4021 accgctctat gttaccggga tcgatgcggt gacccgccag gtgctggtcg gtccgcgccg
     4081 gatgctggca gtcagcgccg cgcatctggt cgaaaccaac cggatcggcc cgctggacga
     4141 tctgccgctg gtggccaagg tccgttcgtt ggccaagcct gtcccggtcg tgctcgacgg
     4201 ccctttgggc gacggcgcac caaccacgat ccgcttcatc aatcccgaat acggcgttgc
     4261 ccccggccag gccgcggtgc tctatgccgg cgagcgggtg gttggcggcg gctggatcga
     4321 gacgaccgaa ccggcagttt cgcaaagcct atagcggctg ggcgatcagg cacagtccgt
     4381 ctgcggcgag ttcggccagc gagggagcca gcgcgcattg accaggctga accgctagat
     4441 cgccgaccct tgccgcgcaa tcgatcggga tggccagaac cggcccggca aagcgcgcgg
     4501 ccagatcagc cgatgccggg ccatcgagcc gtgccagccg gaagtgcggc ccatcgacca
     4561 gcacgatctc gccccgttcg gaaagccttt ggcgcagcgc gggcggatag ggttcgcccc
     4621 gggccacagc aataccggca tcgagatgca gttcgcgcgg gcgaccgtag tcgtaaagcc
     4681 gataggttat gtcgctgttc tgctggacct cgatcagccg cacccccggc ccgatcgcgt
     4741 gaaccgttcc ggccgggatg tagaaaaagt ccccggcctc gacctcatgc caggtaagca
     4801 gctgttcgat cgagccatcg agcgcggcag cgcgcatcgc ttcttgatcg agcgcccggt
     4861 tgaagccgat gcccagcctg gccccggcct cggcaccgat gaccagccag cattcttcct
     4921 tcccgcgtga gctgagtccc tgcgcgcgtg cctgggcatt gctgggatgg acctgcaccg
     4981 aaagcgcggc agaggtgaag atgtatttga ccagcagctg atcgagcgcg gcgggcggaa
     5041 tgaaccacac ctcgccaatc cgcttgccct ggggcgtgac gaacggcgcg ggcagttcat
     5101 ccacccccca ggggcgctcg tcataatggc gggccagcag tacgctcact ggttcgccgc
     5161 gccatccagc ttgccgaccg cctgggcgcc cgcacgggtg gtaaccagca cctcgttgcc
     5221 atcgaccacg atcaccacgt cctcaagccc gatcaccgaa acgcgcggtc catcgctttc
     5281 gaccatgaca tttcggcact cgaccagttc ggcgctcccg cgcacgcagt tgccggccct
     5341 gtcgtgttcg cgcgccgcat agagcgcatc ccagttgccg atgtccgacc agcccatcgc
     5401 agccgggacc atcgcggcgc gggcggtgtt ttccatca
//
LOCUS       DASUDV010000036         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1481364,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000036 DASUDV010000000
VERSION     DASUDV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481364"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFV30_00745"
     CDS             <1..468
                     /locus_tag="VFV30_00745"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX4846643.1"
                     /translation="LGTASQLKVMTNYLCTVHLVALAEALTTCKAAGLDMNTTYEAIR
                     ISSGNSFVHETESQVILNGSRDINFTMDLVSKDIGLFDEIAQEHGVPVELSPLIVRLF
                     KEARAQYGDREFSPNIIRRFEEPLGIKVLGTGFPDQMVDDEPEEPGYEVVPRR"
     gene            465..1967
                     /locus_tag="VFV30_00750"
     CDS             465..1967
                     /locus_tag="VFV30_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4846644.1"
                     /translation="MSSHPASHAEWQALAAGMAFETGLLIDGQSVAGSGTPIAAVNPA
                     TGDALAELQCGTAADIDRAAASGLAAWRDRRWRGMTPRARMDVFRRWADLVEAHAAEL
                     ALLETLSMGKPIGDALGVDLPETVVTIRYFGEAIDKVAGKVTTSPDNATQLIMREPLG
                     LVGAISAWNYPLLMATWKVAPALAAGNCVLLKPSERAGLSCIRLAQLFLEAGGPPGVF
                     NVVNGLGQDAGAALARHPDVAKISFTGSTATGKRLMVMAGESNMKRVSLETGGKSPQI
                     FMPDLADLDTAVDRAIIGIFDNAGQVCNAGSRLLVHRSIHDAFMERFVARTAELYRPG
                     DPLDPGTSLGPLVDHAHQQAVLGWIDRGRGEGASVALGGTADPARPAGAFVEPTLFTG
                     VSPGMAVAREEIFGPVACVLPFETEEEATQIANDSPYGLAASVWTRDLTCAHRMIKAI
                     EAGVVWVNCFGDGDMTQPFGGYKQSGNTRDKSLECLDGYMQSKAAWIALD"
     gene            1990..>3269
                     /locus_tag="VFV30_00755"
     CDS             1990..>3269
                     /locus_tag="VFV30_00755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4846645.1"
                     /translation="MKRLAFALSLLALAPLAAAQAQQAPQPPLSAAESDPVKLGWMQG
                     SPPPPDKRIAFADGSFLVFPRTRWSFAHYRHLFPTAAVSRGTGPVAPLPLALRPDLAR
                     VTFIPTGKSAPMNWVDAFDAGYGDAIVVLHRGRIVFEKYNGVMNPAQPHIGFSVTKSL
                     YGTLTELLIAEGRIDETKTVGHYLPELASSGFGDATVRQVLDMTTSLDYNEDAPDADQ
                     KMYQFSLAAGTYPRPAGYTGPTNTFDLLKTVKKVAPHGERFDYQSVDTEVLGFVIARV
                     MGKPAEKVLEERIWSRLGAEHDSNIVLDEAGMSRTTGGLSATARDLARFGEMMRRGGW
                     FNGRQIVPAAVVAKIRQGGSKKDFANAIWDYDTRRGWSYKSQWWHTHNRNGAYMAIGL
                     FGQAIYIDPAAEMVVVRFMSGPVASTVGMDYLTLP"
BASE COUNT          551 a         1089 c         1119 g          510 t
ORIGIN      
        1 ctgggcacgg cctcgcagct caaggtgatg accaactacc tctgcacggt ccacctcgtc
       61 gcgctggccg aagcgctgac cacctgcaag gccgccgggc tggacatgaa caccacctac
      121 gaggcgatcc ggatctcgtc gggcaacagc tttgtccacg aaaccgaaag ccaggtgatc
      181 ctgaacggca gccgcgacat caacttcacg atggacctgg tcagcaagga catcggcctg
      241 ttcgacgaga tcgcgcagga acacggcgtg ccggtggaac tctcgccgct gatcgtgcgg
      301 ctgttcaagg aagcccgcgc ccagtacggc gaccgcgagt tttcgcccaa catcatccgc
      361 cgctttgaag agccgctggg catcaaggtg ctgggcaccg gcttccccga tcagatggtc
      421 gacgacgagc cggaagagcc gggatacgaa gtggttccgc gccggtgagc agccaccccg
      481 ccagccacgc cgaatggcag gctctggccg ccggcatggc ctttgaaacg ggcctgctga
      541 tcgatgggca gtccgtggcg ggcagcggca ctccgatcgc ggcggtcaat cccgcgaccg
      601 gggatgctct ggccgagctg cagtgcggga ccgccgccga tattgaccgg gcggcggcat
      661 cgggactggc ggcctggcgc gaccggcgct ggcgcgggat gaccccgcgg gcacggatgg
      721 acgtgttccg ccgctgggcc gatctggtgg aggcccatgc cgccgaactg gcgctgctcg
      781 agacgctgtc gatgggcaaa ccgatcggcg acgcgctggg cgtggacctg cccgaaacgg
      841 tggtgacgat ccgctatttc ggcgaggcga tcgacaaggt ggcgggcaag gtcaccacca
      901 gcccggacaa tgccacccag ctgataatgc gcgaaccgct ggggctggtc ggggcgattt
      961 cggcgtggaa ctatccgctg ctgatggcga cctggaaagt cgcaccggcg ctggccgcgg
     1021 gcaactgcgt cctgctcaag ccgtcggagc gcgcgggcct gtcgtgcatc cggctggccc
     1081 agctgttcct ggaagccggc ggccctccgg gcgtgttcaa cgtggtcaac gggctggggc
     1141 aggatgccgg cgccgcgctg gcccggcacc ccgatgtcgc aaagatcagc ttcaccggct
     1201 ccaccgccac cggcaagcgg ctgatggtga tggccggcga aagcaacatg aagcgggtct
     1261 cgctggaaac cggcggcaag agcccgcaga tcttcatgcc ggatctggcc gatctggata
     1321 cggcggtgga ccgcgcgatc atcggcattt tcgacaacgc cgggcaggtc tgcaacgccg
     1381 ggtcgcggct gctggtgcac cgttcgatcc acgatgcatt catggagcgc ttcgtggccc
     1441 gcacggcgga actgtaccgg ccgggcgatc cgctcgatcc gggcaccagc ctggggccgc
     1501 tggtcgacca tgcccaccag caggcggtgc tgggctggat tgaccgcggg cggggcgagg
     1561 gcgcgtccgt ggcgctgggc ggaacggccg acccggcccg cccggcaggg gcctttgttg
     1621 agccgaccct gttcaccggg gtgagccccg gcatggccgt ggcgcgcgag gagatctttg
     1681 gcccggtggc ctgcgtcctg ccgttcgaga ccgaggagga ggcgacccaa atcgccaacg
     1741 attcccccta tggcctcgcc gccagcgtct ggacccgcga tctgacctgc gcccaccgca
     1801 tgatcaaggc gatcgaggcc ggggtggtct gggtcaactg ctttggcgat ggcgacatga
     1861 cccagccgtt cggcggttac aagcaatcgg gcaacacccg cgacaaatcg ctggaatgcc
     1921 tcgatggcta catgcaatcc aaggccgcct ggatcgcgct ggactgatcc gaaccaaggg
     1981 agtgagcgca tgaagcgact ggcctttgcc ctgagcctgc tggcgctcgc tccgctggcg
     2041 gcggcccagg cccagcaagc cccgcagccc ccgctcagcg cggcggaatc cgatccggtc
     2101 aagctgggct ggatgcaggg ctccccgccg ccgccggaca agcggatcgc ctttgccgac
     2161 ggcagcttcc tcgtcttccc caggacccgc tggtccttcg cccattaccg ccacctgttc
     2221 ccgaccgcgg cggtctcgcg cgggaccggt ccggtcgcgc cgttgccgct ggcgctgcgc
     2281 cccgacctgg cccgggtgac cttcattccg accggcaaga gcgcgccgat gaactgggtc
     2341 gatgccttcg atgccggcta cggcgatgcg atcgtcgtgc tgcaccgcgg gcggatcgtg
     2401 tttgaaaagt acaacggcgt gatgaacccg gcgcagccgc acatcggctt ttcggtcacc
     2461 aagtcgctct acggaaccct gaccgaactt ctgatcgccg aggggcggat cgacgagacg
     2521 aagacggtcg gccactacct tccggaactc gccagcagcg ggtttggcga tgccacggtg
     2581 cgccaggtgc tcgatatgac caccagcctc gattacaacg aggacgcgcc cgacgccgat
     2641 cagaagatgt accagttctc gctggccgcc gggacctatc cgcgcccggc cggctacacc
     2701 gggccgacca acacctttga cctcttgaag acggtcaaga aggtcgcccc ccacggagag
     2761 cggttcgatt accagtcggt cgataccgag gtgctgggct ttgtcatcgc ccgggtgatg
     2821 ggcaagccgg ccgagaaggt gctggaagag cggatctggt cccggctggg ggccgagcac
     2881 gattccaata tcgtgctcga cgaagccggc atgtcgcgca ccaccggcgg gcttagcgcc
     2941 actgcgcgcg atctggcccg gttcggcgag atgatgcgcc gcggcggctg gttcaacggc
     3001 cgccagatcg tgccggcggc ggtcgtcgcc aagatccgcc agggcggtag caagaaggac
     3061 ttcgccaacg cgatctggga ttacgacacc cgccgcggct ggagctacaa gagccagtgg
     3121 tggcacaccc acaaccgcaa cggcgcctat atggcgatcg gcctgttcgg gcaggcgatc
     3181 tatatcgatc cggcggcgga aatggtcgtg gtgcggttca tgtccggccc ggtcgcatcg
     3241 accgtgggga tggactatct gacgctgcc
//
LOCUS       DASUDV010000037         5131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_774505,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000037 DASUDV010000000
VERSION     DASUDV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774505"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFV30_00760"
     CDS             <1..222
                     /locus_tag="VFV30_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4846646.1"
                     /translation="FAGPYQGFGLIVIIEHGGGWTSLVTGLAALDTRVGRSVLSGSPL
                     GVAGPGRPVLTLELRSGGTPVNPLDYLRR"
     gene            305..1639
                     /locus_tag="VFV30_00765"
     CDS             305..1639
                     /locus_tag="VFV30_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4846647.1"
                     /translation="MKLSSLLRATALVSAVALIPATTAGLAAVDGRSSAQFERLATVY
                     QLVKLRYVEKVDDKKLEEGAIDGMLASLDPHSSYLSGASMQRLNTMIDGNYSGLGLSV
                     VMDDGAVKVVAPMKGSPAEAAGVKAGDYITHLDGKLIYGGELDDAVAQMRGEAGTKIR
                     LTIFRSGRNEPFDVTVTRGVIKLEPVTWELKDGVGVISVNEFGADVGTDVAKAMDDLK
                     KQSGGKMFGIVLDLRQNPGGSLEEAVALSDIFLTKGDIVSQRGRNPREDEYFKAESMI
                     PGDLAKGLPVIVLIDAGSASASEIVAGALQDQRRAVIMGERSFGKGSVQSLYFLDSSR
                     TSAVKITTARYYTPLGRSVQEGGILPDITVPQLSDPDARRRAEFSIRESDLRRHLVNE
                     VGMDDKKLEADRQQDPRFKMTAEELKAKGIKDFQLYYAIQTLRRTTPPARKR"
     gene            1642..2118
                     /locus_tag="VFV30_00770"
     CDS             1642..2118
                     /locus_tag="VFV30_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX4846648.1"
                     /translation="MTAAAPSPGLLARGVALGVPLALLGGAYVSQYVFGLPPCEMCWW
                     QRYALFAALPFALIAWFRPSARPATALALLAILAGGLIGGFHAGVEYGWWEGLTACTT
                     TAAADGGNALDAILDAPIIRCDVAPWDLFGISLAGWNFLISVPLALIGLANLRKGA"
     gene            2123..2638
                     /locus_tag="VFV30_00775"
     CDS             2123..2638
                     /locus_tag="VFV30_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX4846649.1"
                     /translation="MTTTASARMLRVDQAGEYGATRIYAGQLAVLGDRTPISAEIRHM
                     AEQEAVHRAKFDALIARRGVRPTALQPFWDVAGFALGAATALIGPKAAMACTAAVETE
                     IDRHYTQQLEDLGEDDPELSAMIAEFREDERAHRDAALAAGAEQAPAYPLLSGAIRLG
                     CRVAIRLAERI"
     gene            2760..3320
                     /locus_tag="VFV30_00780"
     CDS             2760..3320
                     /locus_tag="VFV30_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846650.1"
                     /translation="MRPLFAALPLAVAALAAAPAAAQEAPAEPKMNMVIVYGNDKCPE
                     SNDDTITVCARKAESERYRIPEPLRTSSAPSNEAWNNRVLAYETVGRSGIASCSPAGA
                     GGWTGCTDKLLDQAYGEKRTDPGIRFGQLIAEARAKRLETIDEDAAETQSRVEEVEKA
                     MEAKARAEEEAKAKGQPQPPALPEPK"
     gene            complement(3340..4143)
                     /gene="fabI"
                     /locus_tag="VFV30_00785"
     CDS             complement(3340..4143)
                     /gene="fabI"
                     /locus_tag="VFV30_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX4846651.1"
                     /translation="MTGLMQGKRGLIMGLANDKSLAWGIAQKLHEQGAELAFSYQGEA
                     LEKRVRPLAASLGSDFLIDCDVSDMAALDTAFATLAARWPTIDFVVHAIGFSDKNELR
                     GRYYDTSLDNFLMTMNISAYSLVAVTRRAKDMMPNGGSILTLSYYGAEKVVPHYNVMG
                     VAKAALETSVKYLANDLGPENIRVNAISAGPIRTLAASGIGDFRYILKWNELNSPLRR
                     NVTIEDVGGSALYLLSDLSSGVTGETHHVDAGYHLIGMKQEDAPDIALS"
     gene            complement(4140..5024)
                     /locus_tag="VFV30_00790"
     CDS             complement(4140..5024)
                     /locus_tag="VFV30_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX4846652.1"
                     /translation="MIARVWTGAYHDGFIHAGNLAYMAILALFPFFITVGAVFSLVGE
                     QAQRAASVHTVLKALPPVVASALEPVAMDVIVARSGWLLWIGGLVGLWTVGSLIETIR
                     DILRRAYGTRETHAYWRYRLISTGVIFGAVIALLFALLAQVAIGTAQEVIQAWFPRAA
                     GWIDTLGTSRLVPALVIYLALYLLFFTLTPTAYRKRRYPKWPGALLVTAWWAAVSAAL
                     PAVLHRFFTYDLTYGSLAGVMISLFFFWLVGLGMVVGAELNAALAESPEEQDLIGRWR
                     ERDRQEAAAGTATTGTNE"
BASE COUNT          847 a         1752 c         1727 g          805 t
ORIGIN      
        1 ttcgcggggc cctatcaggg gttcgggctg atcgtgatca tcgagcacgg cggcggctgg
       61 acctcgctgg tcaccggcct tgccgcgctc gacacgcggg tcggccgctc ggtcctgtcc
      121 ggatcgccgc tcggcgtggc cgggccgggc cgtccggtcc tgacgctcga gctgcgcagc
      181 ggcggcaccc cggtcaaccc gctcgactac ttgaggcgct gagcggacgg cgcacgcatc
      241 atctggcatt caggcgcgcg gcaggatatg ctgcaacccg acatcacaag aggcacctgg
      301 ccccatgaag ctttcttccc tgctccgcgc gacggccctg gtttccgcgg tcgcgctcat
      361 tcccgccacc acggccggcc tggccgcggt cgacgggcgc tcttcggccc agttcgaacg
      421 gctcgccacg gtctaccagc tggtcaagct gcgctatgtc gaaaaggtcg acgacaagaa
      481 gctggaagaa ggcgcgatcg acggcatgct ggcctcgctc gatccgcatt cgtcctatct
      541 ttcgggcgcg tcgatgcagc ggctcaacac catgatcgac ggcaactatt cgggccttgg
      601 cctgtcggtc gtgatggatg acggcgcggt caaggtggtc gccccgatga agggcagccc
      661 ggccgaagcc gccggggtca aggccgggga ctatatcacc cacctcgacg gcaagctgat
      721 ctacggcggc gagctggacg acgcggttgc ccagatgcgc ggcgaggccg gaaccaagat
      781 ccgcctgacg atcttccgct cgggccgcaa cgaacccttc gacgtcaccg tgactcgcgg
      841 cgtgatcaag ttggagccgg tgacctggga gctgaaggac ggcgtcgggg tcatttcggt
      901 caacgaattc ggcgccgatg tcggcaccga cgtggccaag gcgatggacg acctcaagaa
      961 gcagtccggc ggcaagatgt tcggcatcgt gctcgacctg cgccagaacc cgggcggctc
     1021 gctggaagag gcggtggccc tgtcggacat cttcctgacc aagggcgaca tcgtctcgca
     1081 gcgcggccgc aacccgcgcg aggacgaata cttcaaggcc gaatcgatga tcccgggcga
     1141 tctcgccaag ggtctgccgg tgatcgtgct gatcgacgcg ggctccgcct cggcgtccga
     1201 aatcgtcgcc ggggcgttgc aggatcagcg ccgcgccgtg atcatgggcg aacgcagctt
     1261 tggcaagggt tcggtccagt cgctttattt cctcgattcg tcgcggacca gcgcggtcaa
     1321 gatcaccacg gcgcgctatt acaccccgct tggccgctcg gtgcaggaag gcgggatcct
     1381 gcccgacatc accgtgccgc agctgtctga tccggacgcc cgccgccggg ccgaattctc
     1441 gatccgcgaa agcgatctgc gccgccacct cgtcaacgaa gtgggcatgg acgacaagaa
     1501 gctggaggcc gaccgccagc aggatccgcg cttcaagatg accgcggagg aactgaaggc
     1561 caaggggatc aaggacttcc agctctacta cgcgatccag accctgcgcc gtaccacgcc
     1621 gccggcccgg aagcgctgag catgaccgct gccgcgcctt cgcccgggct gctggcccgg
     1681 ggtgtcgctc tgggcgtgcc gctggcgctg ctcggcggag cctatgtgag ccagtacgtg
     1741 ttcggcctgc cgccgtgcga gatgtgctgg tggcagcgtt acgccctgtt cgccgcgctg
     1801 ccctttgccc tgatcgcctg gtttcgcccc tccgcgcggc cggccaccgc cctggccctg
     1861 ctggccatcc tggccggcgg gctgatcggg ggctttcacg ccggggtcga atacggctgg
     1921 tgggaagggc tgaccgcttg caccaccacg gccgcggcgg acgggggcaa cgcgctcgac
     1981 gcgatcctcg atgccccgat catccgctgc gatgtcgccc cgtgggatct gtttggcatt
     2041 tcgctggcgg gctggaactt cctgatctcg gttccgctcg ccctgatcgg cctcgccaac
     2101 ctgcgcaagg gagcctgaca ccatgaccac gaccgcttcg gcccggatgc tgcgggtgga
     2161 ccaggccggc gaatacggcg cgacccggat ctatgccggc cagctggccg tgctggggga
     2221 tcggaccccg atctcggccg agatccgcca catggccgaa caggaagcgg tgcaccgcgc
     2281 caagttcgac gcgctgatcg cccggcgcgg cgtgcgtccc acggcgctgc agccgttctg
     2341 ggacgtggcc ggctttgccc tgggcgcggc caccgcgctg atcgggccca aggcggcgat
     2401 ggcgtgcacc gccgcggtcg agaccgagat tgaccggcat tacacccagc agctcgagga
     2461 tctgggcgag gacgatccgg agctgtcggc aatgattgcc gaattccgcg aagacgagcg
     2521 cgcccaccgc gatgcggccc tggcggccgg tgcggaacag gccccggcct atccgctgct
     2581 gtcgggcgcg atccggctgg gctgccgggt ggcgatccgg ctggccgagc ggatctgagc
     2641 gccgcgcccc gcattttgat gcagggcgtt ttgacccacg acaaggcgcg gcccgcttca
     2701 tctgttattc agcgccaagg ctgcctataa gggtggtcga cgttttcagg agtcttgcca
     2761 tgcgcccgct gtttgccgcc ctgccgcttg ccgtggccgc gcttgccgcc gcgcccgccg
     2821 ccgcccagga ggccccggcc gagcccaaga tgaacatggt gatcgtctac gggaacgaca
     2881 aatgtcccga atcgaacgac gacacgatca ccgtctgcgc gcggaaggcc gaaagcgagc
     2941 gctaccgcat tcccgaaccg ctgcggacca gttcggcccc gtcgaacgag gcatggaaca
     3001 accgggtgct ggcctatgaa acggtcggcc ggtcgggcat tgccagctgc tcgcccgccg
     3061 gggccggggg ctggaccggc tgcaccgaca agctgctcga ccaggcctat ggcgaaaagc
     3121 gcaccgatcc gggcatccgc tttggccagc tgatcgccga agcccgggcc aagcggctcg
     3181 agacgatcga cgaggacgct gccgagaccc agtcgcgggt cgaggaggtg gaaaaggcga
     3241 tggaagccaa ggcccgcgcc gaggaagagg ccaaggccaa gggccagccc cagccgccgg
     3301 ccctgccgga acccaagtaa gggcccgcgc gctcccgcct taagagagcg cgatgtccgg
     3361 ggcgtcttcc tgcttcatcc cgatcaggtg atagccggca tcgacgtggt gcgtctcgcc
     3421 ggtgaccccc gatgacaggt ccgacagcag gtacagcgcc gaaccgccga catcctcgat
     3481 cgtcacgttg cggcgcagcg gcgagttgag ctcgttccac ttcaggatgt agcggaaatc
     3541 gccgatcccg ctggcggcca gggtccggat cggaccggcc gagattgcgt tgacgcggat
     3601 gttttccggc cccaggtcgt tggcgaggta tttcacgctg gtttccagcg ctgccttggc
     3661 cacgcccatc acgttgtagt ggggcacgac cttttccgcg ccgtaatagc tcagggtcag
     3721 gatcgaaccg ccattcggca tcatgtcctt ggcccgccgg gtcaccgcga ccagcgaata
     3781 ggccgagatg ttcatggtca tcaggaagtt gtcgaggctg gtgtcgtaat accgcccgcg
     3841 cagttcgttc ttgtcggaaa agccgatcgc gtggaccacg aaatcgatcg tcggccagcg
     3901 cgccgcaagg gtggcaaagg cggtgtccag cgcggccatg tcggacacgt cgcaatcgat
     3961 caggaaatcg ctgcccaggc tggcggccag cggccggacc cgcttttcca gcgcttcgcc
     4021 ctggtaggaa aaggccagct cggcgccctg ttcgtgcagc ttctgggcaa tcccccaggc
     4081 cagcgacttg tcgttcgcca ggcccatgat cagcccgcgt tttccttgca tcaatccggt
     4141 cattcgttcg tccccgtcgt cgccgtgccg gcggctgcct cttgccgatc acgctcccgc
     4201 cagcggccga tcaggtcctg ctcttcggga ctttcggcca gggcggcgtt caattcggca
     4261 cccaccacca ttcctaggcc aaccagccag aaaaagaaaa gcgagatcat cacgcccgcc
     4321 aggctgccgt aggtcagatc gtaggtgaag aagcggtgca gcaccgccgg aagcgcggcg
     4381 ctgaccgcgg cccaccaggc cgtgaccagc agcgcgccgg gccacttggg atagcgccgc
     4441 ttgcggtagg cggtgggggt cagcgtgaag aacagcaggt agagcgcgag gtagatcacc
     4501 agcgccggca ccagccgcga ggtgcccagc gtgtcgatcc agccggccgc gcgcgggaac
     4561 caggcctgga tcacctcttg cgccgtgccg atcgccactt gcgccagcag cgcgaacagc
     4621 agcgcgatca ccgcgccaaa gatcaccccg gtcgaaatca gccggtagcg ccaataggca
     4681 tgggtctcgc gcgtgccata ggcgcggcgc aggatgtcgc ggatcgtctc gatcaggctg
     4741 cccaccgtcc acagcccgac cagcccgccg atccacagca gccagccgct gcgggccacg
     4801 atcacgtcca tcgcaaccgg ctccagcgcc gacgcgacga ccgggggcag ggctttcagg
     4861 acggtgtgga ccgaggctgc gcgctgggcc tgctcgccga ccaggctgaa caccgcgccg
     4921 accgtgatga agaacggaaa cagcgccagg atcgccatgt aggccaggtt gccggcgtgg
     4981 atgaagccgt cgtgataggc cccggtccag acccgggcga tcacccggac cgggcgcgat
     5041 tgccagaacc ggccgtgcgg gccgacatgc gcgtcatagg tgtcgcgcag ccgccgggcc
     5101 cgctcgcgcc gggcctcggg cgagaggtcc g
//
LOCUS       DASUDV010000038         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1658078,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000038 DASUDV010000000
VERSION     DASUDV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658078"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFV30_00795"
     CDS             complement(<1..634)
                     /locus_tag="VFV30_00795"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HEX4846653.1"
                     /translation="MASRPNAPLPTLRDLLARANLRLILFSVLLATLSLVLSGGLVIR
                     NYAQRNLELVARTVAYTVEPAVLFDDREAIREGIVSVAAGRGVERVEVHDARGRLLAD
                     WQSPETGPLAAISRVANTVIWPDPAVDQVHRGDQHIAEVRIYGNSEGILRYALVGAII
                     AAVCLALTLLATRILARRLQSEVVLPLEHVANVAHAVREERAFDKRVPSAG"
     gene            complement(637..1179)
                     /locus_tag="VFV30_00800"
     CDS             complement(637..1179)
                     /locus_tag="VFV30_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX4846654.1"
                     /translation="MKSTVTLLALPLLVAAAPPILAMADVPVGASVAGQSRYAGAVAR
                     TVRAIIEYTRWPQRSDPVVLCVAGPAQHASQLGSIRLSDGRRVERRNVAPNAAALGGC
                     HVLYIGQVPLAQQRQLTSAVRGRAVLTIAEADPGTASEAMFALTFKPAALSFRLNIDA
                     VSRSGLKVDPRVLRLAQGGL"
     gene            1305..>3752
                     /gene="putA"
                     /locus_tag="VFV30_00805"
     CDS             1305..>3752
                     /gene="putA"
                     /locus_tag="VFV30_00805"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX4846655.1"
                     /translation="MLGQIDRSALRAAYRMEEEACLAERIRQAAPASAVHGEASRIAA
                     RLVEGARQRKASGLDAFLQTYGLGTDEGIALMCLAEALLRVPDAATADALIHDKLGGI
                     DWSDHVGESSSTFVNAATFSLMLTGQVLSQKQAGGFGASLKRAVSRLGEPVIRTATLQ
                     AMKILGGQFVFGRTIDEALQRAAPERKQGLTHSFDMLGEAAMTFDDAERYRRSYEGAL
                     ERIAQEAGDGVVRSPGISVKLSALFPRYDFLHAEAARAVLVPMLKELALKAAAADIHF
                     TIDAEEAERLELSMDIIEELVADPDLFATGWQGFGLALQAYSKRAVPLVEWVTALGRK
                     HHRRIMVRLVKGAYWDSEVKLSQVGGHGDYPVFTRKVATDVSYLACAAKLLAAPDAIY
                     PAFATHNAYTVGAIKALAGTTPFEFQRLHGMGEDVYDELAKLEAEAGQPRTPVRIYAP
                     VGGHKDLLAYLVRRLLENGANSSFVNRMADAEVPVADLVGDPVAELAALSPRRNPAIP
                     LPRDIFPGRVNSAGVDLADPLVRGALGESLRALDPIEWAFPPASGSARPVRSPYDSQR
                     LVGTVADATPADVDAAIAAAEAAQPAWDALGGEGRAALLDAAADLYEARTEEFLSLCQ
                     REAGKTLVDAVLELREAVDFLRYYAAEARRLFTPEGEALPGPTGESNRLTLHGRGAFV
                     AISPWNFPLAIFTGLVAGPLAAGNTVIAKPAEQTPLIAAYAVQLLREAGIPDGAVQLV
                     LGDGKVGALLTADPRIAGVAFTGSTETARAINRSLAGRDGPLPILVAETGGQNAMIVD
                     SSALPEQVTRDVVASAFQ"
BASE COUNT          592 a         1319 c         1292 g          549 t
ORIGIN      
        1 tcccggccga gggcacgcgc ttgtcaaagg cgcgctcctc gcgcaccgca tgggccacgt
       61 tggcaacatg ctccagcggc agcaccactt cgctttgcag acgccgggcc aggatccggg
      121 tcgccagcag ggtcaaggcc aagcagaccg cggcgataat cgcgccgacc agggcatagc
      181 gcaggatccc ttccgaattg ccgtagatcc gcacctcggc aatgtgctgg tcgccgcggt
      241 gcacctggtc caccgccgga tcgggccaaa tcacggtgtt ggcaacccgg ctgatggcgg
      301 cgagcggccc ggtttccggg ctctgccagt cagccagcag ccgccccctg gcatcgtgga
      361 cttcgacccg ctccaccccg cgcccagccg ccaccgagac gatcccttcc cgaatcgcct
      421 cacggtcatc gaacaggact gccggctcca ccgtataggc cacggtccgg gcgaccagtt
      481 ccaggttgcg ctgggcatag ttccggatca ccagcccgcc gctcaacacc aggctgagcg
      541 tggcgagcag cacgctgaac aggatcaggc gcaggttggc gcgggcgagc aggtcgcgca
      601 gggtgggcag cggggcgttc ggccgggacg ccatggtcac agcccccctt gcgccaggcg
      661 caggacccgc ggatcgacct tcagacccga acgcgacacc gcgtcgatgt tgagccgaaa
      721 gctcagcgcg gcgggcttga aggtcagcgc aaacatcgcc tcgctggcgg tgcccggatc
      781 ggcttcggca atggtcagca ccgcccggcc gcggaccgcg gaggtcagct ggcgctgctg
      841 ggccagcggc acctggccga tgtagagcac gtggcaccca cccagcgccg ccgcgttggg
      901 ggcgacgttg cggcgttcaa cccggcggcc gtcgctcagc cggatcgatc ccagctgcga
      961 cgcgtgctgc gccggaccgg ccacgcacag caccaccgga tcgctgcgct gcggccagcg
     1021 ggtgtactcg atgatcgccc gaaccgtgcg ggccaccgct ccggcatagc ggctttgccc
     1081 ggctacgctg gcgcccaccg gcacgtccgc catcgccagg atcggcgggg cggcggcaac
     1141 cagcaggggc agggccaaaa gggttacggt cgatttcacc cgtcagcctg tagcgattta
     1201 agggtaaacg aaaaatagcg gaatttcgcg gatcaggcta agtttttcta caggttgccc
     1261 cgccggattg gtttccggtg caaccaatgc tagacgcctg tcccatgctt ggacagattg
     1321 accgttccgc cctgcgtgcc gcctaccgca tggaagagga ggcctgcctg gccgaacgaa
     1381 tcaggcaagc cgccccggcc agcgccgtcc acggcgaagc cagccggatc gcggcgcggc
     1441 tggtcgaggg tgcgcggcag cgcaaggcca gcgggctcga cgccttcctg cagacctatg
     1501 gcctgggcac cgacgagggg atcgcgctga tgtgcctggc cgaggcgctg ctgcgggtgc
     1561 ccgatgcggc caccgccgac gcgctgatcc acgacaagct gggcgggatc gactggtccg
     1621 accatgtcgg ggaaagcagc agcaccttcg tcaacgcggc caccttctcg ctgatgctga
     1681 ccggccaggt gctcagccag aagcaggccg gcggctttgg cgcctcgctc aagcgcgcgg
     1741 tcagccggct gggcgaaccg gtgatccgca ctgccacgct tcaggcgatg aagatcctgg
     1801 gcggccagtt cgtcttcggc cggaccatcg acgaagcgct gcagcgcgcc gcgcccgagc
     1861 gcaagcaggg cctgacccac agcttcgaca tgctgggcga agcggcgatg acctttgacg
     1921 acgccgaacg ctatcgccgt tcctacgagg gcgcgctgga gcggatcgcc caggaagccg
     1981 gggacggcgt ggtccgctcg ccgggcattt cggtcaagct ctccgccctg tttccgcgct
     2041 acgatttcct ccacgccgag gccgcccgcg ccgtgctggt gccgatgctg aaggagctgg
     2101 cgctgaaagc cgcggcggcc gacatccatt tcacgatcga cgccgaagag gccgagcggc
     2161 tggaactgtc gatggacatc atcgaggaac tggtcgccga tccggacctg ttcgccaccg
     2221 gctggcaggg ctttggcctg gccctgcagg cctattccaa gcgcgccgtg ccgctggtcg
     2281 aatgggtcac ggcgctgggg cgcaaacacc accgccggat catggtccgg ctggtcaagg
     2341 gcgcctactg ggacagcgag gtgaagctca gccaggtcgg cgggcacggc gactatccgg
     2401 tcttcacccg caaggtcgcg accgacgttt cgtaccttgc ctgcgcggcc aagctgctgg
     2461 ccgcgcccga tgcgatctat cccgcctttg ccacccacaa cgcctatacc gtgggtgcga
     2521 tcaaggcgct ggcggggacc actccgttcg aattccagcg cctccacggc atgggcgaag
     2581 acgtttacga cgagctggcg aaactggagg ccgaggccgg gcagccccgc accccggtgc
     2641 ggatctatgc ccccgtcggc gggcacaagg acctgcttgc ctatctggtc cgccgcctgc
     2701 tggaaaacgg cgcgaacagc agcttcgtca accgcatggc cgatgccgaa gtgccggtgg
     2761 cagacctggt gggcgatccg gtggcggagc tggcggcgct gtccccgcgc cgcaatccgg
     2821 cgatcccgct gccgcgcgat atcttcccgg ggcgggtcaa cagcgcgggc gtcgatctgg
     2881 ccgatccgct ggtgcgcggc gcgctgggcg aaagcctgcg cgcgctggac ccgatcgaat
     2941 gggcctttcc tccggccagc ggatcggcgc gcccggttcg ttccccctat gacagccagc
     3001 ggctggtcgg cacggtggcc gatgccacgc cggctgacgt cgatgccgcg atcgctgccg
     3061 ccgaggcggc ccagccggcc tgggatgcgc tgggcggcga aggccgggcc gcgctgctcg
     3121 acgccgcggc cgacctttac gaggcgcgga ccgaggaatt cctctcgctg tgccagcgcg
     3181 aggccgggaa gaccctggtc gatgccgtgc tggagctgcg cgaggcagtc gatttcctgc
     3241 gctattacgc cgccgaagcg cgccgcctgt tcacgcccga gggggaagcg ctgcccgggc
     3301 ccacgggcga aagcaaccgc ctgaccctcc acgggcgcgg ggccttcgtg gcgatcagcc
     3361 cgtggaattt cccgctggcg atcttcaccg gcctggtcgc cggaccgctg gcggcgggca
     3421 acaccgtgat cgccaagccg gccgagcaga ccccgctgat cgccgcctat gcggtgcagc
     3481 tgctgcgcga ggccggcatt ccggacggcg cggtgcagct ggtgctgggc gacggcaagg
     3541 tcggcgcgct gctgaccgcc gatccgcgaa ttgccggggt ggcctttacc ggatcgaccg
     3601 aaaccgcccg cgcgatcaac cgcagcctgg ccggccgcga cgggccactg ccgatcctgg
     3661 tggccgaaac cggcgggcag aacgcgatga tcgtcgacag ttcggccctg cccgaacagg
     3721 tcacccgcga tgtcgtcgcc agcgctttcc ag
//
LOCUS       DASUDV010000039         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1768634,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000039 DASUDV010000000
VERSION     DASUDV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768634"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1170)
                     /gene="dnaN"
                     /locus_tag="VFV30_00810"
     CDS             complement(52..1170)
                     /gene="dnaN"
                     /locus_tag="VFV30_00810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX4846656.1"
                     /translation="MKATIERATLLKCLSHVQSVVERRNTIPILSNVLIEAAGDGTVR
                     VMATDLDLQVVESLGAVSVDSPGAITVSAHLLFDIARKLPDGSQVSLETADNRMAVKA
                     GRSRFSLPTLPRDDFPVIVEGDLPTSFELPAATLAQLIDRTRFAISTEETRYYLNGIF
                     IHVTEDELRAAATDGHRLARYTIARPDGAEGMPDVIVPRKCVAELRKLLEEALDASVL
                     IDLSASKIRFTLGGENGVVLTSKLIDGTFPDYSRVIPTGNDKLLKLDPKSFWEGVDRV
                     ATIATEKTRAVKMALENDKVTLSVTSPDNGTAAEEVPASYANTGFEIGFNANYLKDIL
                     NQIEGDTVELHLADAGAPTLIRQDEKSPALYVLMPMRV"
     gene            complement(1321..1872)
                     /locus_tag="VFV30_00815"
     CDS             complement(1321..1872)
                     /locus_tag="VFV30_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846657.1"
                     /translation="MRKLLMLAASAALLGALPAQAKLSEAQAECPALKAPEGLGRLLL
                     EDIGNFQKTRKKNEELGKRIAELAEQCAKETGIAPAEKAQYTLYTSARLGRDEAARQL
                     TAMGIQPAIIDRELDMGPGKRNRPGNELTREEFTKLYGALVASGADSAVLADQGGRMM
                     GYYVQLNAMTFKFIEQLGAPAGN"
     gene            complement(1902..2438)
                     /locus_tag="VFV30_00820"
     CDS             complement(1902..2438)
                     /locus_tag="VFV30_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846658.1"
                     /translation="MRYLAVFGASLALLSAQPALAGISKAAFDCPAANAPAGLAASLA
                     DVIIKGDENSPEVPKVFEALRTLTDTCIAKTGVKPDQAEAYAGYTFDVLLHRELGKRL
                     TAKGVPVEAIDEGLGFGPGRPNLPVGEIQDSHVEKISAAMRVRGYDLAKAPDEVHRLI
                     GMYIGALSGLAEDRAKLN"
     gene            complement(2479..3231)
                     /gene="fabG"
                     /locus_tag="VFV30_00825"
     CDS             complement(2479..3231)
                     /gene="fabG"
                     /locus_tag="VFV30_00825"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX4846659.1"
                     /translation="MFDLTGMTALVTGAAGGIGSAISHALASQGARLALSGTNPAKLR
                     AFREELNDTYGHDHIEITCDLSNTEQVEHLIPATVDTLGKIDILVNNAGITRDNLAMR
                     MKDEEWDAVIRVNLEAAFRLMRAACKPMMKARHGRIITITSVVGATGNPGQVNYAAAK
                     AGLVGMSKSLGQEVASRGITVNCVAPGFIRTAMTDVLPDAQKDALNARIPMGRMGEGS
                     DIGAAVAYLASKEAGYITGQTLHVNGGMAMIS"
     gene            complement(3285..>5258)
                     /locus_tag="VFV30_00830"
     CDS             complement(3285..>5258)
                     /locus_tag="VFV30_00830"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX4846660.1"
                     /translation="LKEGSMARFWMGVVLVAALIGFVPAEPALAKREAMPSVGSDAQL
                     KRLIRRTVMVEDGLMAEAGGPPPVPAPPAPPPMAAPAPMVVSGRAAPPPAPGAEGITN
                     VQTQGIDEGGIVKQRGRNLIVLRRGRLFSIDTEAGGLKLIDTIDAFPPFNGRGGAWYD
                     EMLVSGDWVIVIGYNYARGGTEINRFKLDRLGRFTLVDTYHLRSDDYYSAENYASRLV
                     GNRLVVYAPLPINMRDPLGSLPGMRRWSAENGAKGGFTRIALARDIHVPDLLLNLKRA
                     NLTTYHAVTTCNLTAPVMDCKAQVVLGSYSRTFYVGPDAVYVWTGNLTLRSWRDQSPP
                     DPRGLLYRMPLDGSAPQAAVVWGGPVNQFGMRSDADGNFQALVRSGYGGDAMWEPVRG
                     GRGGLALLSLKSGAFGRGRGKPQRADYTPLPGGEVPTLENRFVGGYLVYAVHGSIGYR
                     DAQGWKNKTFDPMAYALRLDTRKLTPVAPGGAVTRIDQMGGDAILIGTAPGGALRFQA
                     LGLDGPGDPAIIAKYDLPAAREGESRSQAFFFRPQNADGSDGILGLPVALDKPQGMRS
                     ALPYRSGIFYLNRRQRGFVPLGTLSTNPVASANDNCKASCVDWYGNARPIFLGSRVFA
                     LLGYELVEGRAGETAIEEVQRLDFNPAQAQQER"
BASE COUNT          806 a         1758 c         1769 g          925 t
ORIGIN      
        1 agcggcaaag gccagcgcgg ccagagcgaa gggaagaatg gctttcatgg atcagaccct
       61 catcggcatg agcacgtaca gtgcaggact tttctcatcc tggcggatca gggtcggcgc
      121 gccggcgtcg gcgaggtgga gctccaccgt atcgccctcg atctggttca ggatgtcttt
      181 gaggtagttg gcgttgaagc cgatttcgaa gccggtgttg gcatagctgg ccgggacttc
      241 ttcggcggcg gtgccgttgt cgggcgaagt gaccgacagg gtgaccttgt cgttctccag
      301 cgccatcttg accgcgcggg tcttttcggt ggcgatggtg gccacgcggt ccaccccttc
      361 ccagaagctc ttgggatcga gcttcaagag cttgtcgttc ccggtcggga tgacgcggct
      421 gtaatcgggg aaggtgccgt cgatcagctt gctggtcagc accacgccgt tttccccgcc
      481 cagggtgaag cggatcttgc tggccgacag atcgatcagc acgctggcat cgagcgcctc
      541 ttcgagcagc ttgcgcagtt cggccacgca tttgcgcggc acgatcacgt cgggcatccc
      601 ctcggccccg tccgggcggg cgatggtgta acgggccagc cggtggccat cggtggccgc
      661 ggcgcgcagc tcgtcctcgg tcacgtggat gaagatcccg ttgaggtagt agcgggtttc
      721 ctcggtgctg atcgcaaagc gggtccggtc gatcagctgg gccagggtgg cggccggcag
      781 ctcaaagctg gtcggcaggt cgccttccac gatcaccggg aaatcgtcac gcggcagggt
      841 cggcaggctg aagcggctgc gcccggcctt caccgccatg cggttgtcgg cggtttccag
      901 gctgacctgg ctgccatcgg gcagcttgcg ggcgatatcg aacagcaggt gcgccgaaac
      961 ggtgatcgcg ccggggctgt cgaccgagac cgcgccgagg ctttcgacca cctggaggtc
     1021 aaggtcggtc gccatgaccc ggacggtgcc gtcgccggcc gcctcgatca gcacgttcga
     1081 caggatcggg atcgtgttgc ggcgttcaac gacggactgg acgtgcgaca ggcacttcag
     1141 cagcgtcgcg cgttcgattg tggccttcat ggtaccttcg tatccccctg gggcgcgcaa
     1201 aggggaatcg ccccgcgcgc cttggtttgc ggtcaccatt ccttaccgca ggggcctgtg
     1261 gcggcaaggc tcggcccggc gaacggtcct gaaactgggg ataaatcccc tcaggacccc
     1321 tcagttcccg gcaggagcgc ccagctgctc gatgaacttg aaggtcatcg cgttgagctg
     1381 cacgtaatag cccatcatgc gcccgccctg gtccgccaga acagcggagt cggcgcccga
     1441 ggcaaccagc gccccgtaca gcttggtgaa ttcctcccgg gtcagctcgt tccccggccg
     1501 gttgcgcttt ccggggccca tatccagctc gcggtcgatg atcgcgggct ggatgcccat
     1561 cgcggtcaat tgccgtgccg cctcgtcgcg cccgagccgg gctgaggtgt agagtgtgta
     1621 ttgcgccttc tcggccgggg cgatcccggt ttccttggcg cattgctcgg ccagctcggc
     1681 aatgcgtttg ccaagctcct cgtttttctt ccgggttttc tggaagttgc caatgtcttc
     1741 cagaagcagc cggccgagcc cttcgggcgc tttcagcgcc gggcattcgg cctgggcctc
     1801 gctcagtttg gcctgggccg ggagggcccc caacagggcg gcactggccg ccagcatcag
     1861 caatttgcgc atgggttccc tcccccgttt cggatcgcgg ctcagttcag cttggcgcgg
     1921 tcttcggcca ggccgctcag cgcgccgatg tacatgccga tcaggcggtg aacctcgtcc
     1981 ggggccttgg ccaggtcata gccgcgcacc cgcatcgccg cggaaatctt ctcgacatgc
     2041 gaatcctgga tttcgcccac cggcaggttc ggccggccgg ggccaaagcc caacccctcg
     2101 tcgatcgcct cgaccggaac gcccttggcg gtcagccgct tgccgagttc ccggtgcagc
     2161 aggacgtcga aggtatagcc ggcataggcc tcggcctgat cgggcttcac gccggtcttg
     2221 gcgatgcagg tgtcggtcag cgtccgcagc gcctcgaaca ccttgggcac ttcgggcgaa
     2281 ttctcgtccc ccttgatgat cacgtcggcc aggctcgccg caagtccggc cggcgcgttc
     2341 gcggccgggc agtcaaaggc cgccttggaa atgccggcga gcgccggctg ggccgaaagc
     2401 agggcaagcg aagccccgaa aaccgccaga taccgcatta cgggaactcc cctgttggcc
     2461 ggccgtggat tgggccggtc agctgatcat ggccatgcca ccattaacgt gcaatgtctg
     2521 gccggtaata tatccggcct ccttgctggc cagataggcc accgccgcgc cgatatcgct
     2581 gccctcgccc atccggccca tcgggatccg cgcgttgagc gcgtccttct gggcatcggg
     2641 caggacgtcg gtcatcgcgg tgcggatgaa gcccggcgcc acgcagttga ccgtgatgcc
     2701 gcggctggcc acttcctgac ccagcgactt ggacatgccg accaggcccg ccttggccgc
     2761 ggcatagttg acctgcccgg gattgccggt ggcgccgacc acgctggtga tggtgatgat
     2821 ccggccgtgg cgggccttca tcatcggctt gcaggccgcg cgcatcaggc gaaaggcggc
     2881 ttccagattg acgcggatca ccgcgtccca ttcctcgtcc ttcatccgca tcgccagatt
     2941 gtcgcgggtg atcccggcgt tgttcaccag gatgtcgatc ttgcccaggg tatcgaccgt
     3001 ggcggggatc agatgctcaa cctgctccgt gttggacagg tcgcaggtga tctcgatatg
     3061 gtcgtgcccg taggtgtcgt tcagctcttc gcggaaagcg cgcagcttgg ccgggttggt
     3121 gccggacagc gccagccggg cgccctggct ggccagcgcg tggctgatcg ccgagccgat
     3181 gcccccggca gcgccggtca cgagtgcggt cattccggta aggtcgaaca tggcaggcct
     3241 ctttgttgca atcgtcctgc cactcgcgcc ttacgcggcg atagtcaacg ctcctgctgg
     3301 gcctgggcgg gattgaaatc gagccgctgg acctcctcga tcgcggtctc tccggcgcgc
     3361 ccttcgacca attcgtagcc cagcagcgcg aagacccggc tgcccaggaa gatcggccgg
     3421 gcgttgccgt accagtccac gcaggaagcc ttgcagttgt cattggccga ggcgaccgga
     3481 ttggtgctga gcgtgccgag cggcacgaag ccgcgctggc ggcggttgag gtagaagatg
     3541 cccgagcggt agggcagcgc gctgcgcatc ccctgcggct tgtccagcgc caccggcagg
     3601 cccaggatcc cgtcgctgcc atcggcgttc tgcgggcgga agaagaaggc ctggctgcgg
     3661 ctttcccctt cgcgggcggc gggaagatcg tatttggcga tgatcgccgg atcgccaggt
     3721 ccgtccagcc ccagtgcctg gaaccgcagc gcgccgccgg gggcggtgcc gatcaggatc
     3781 gcatcgccgc ccatctggtc gatccgggta accgccccgc ccggcgccac cggggtgagc
     3841 ttgcgagtgt cgagccgcag ggcataggcc atgggatcga acgtcttgtt cttccagccc
     3901 tgggcatcac ggtatccgat cgatccgtgg accgcataga ccagatagcc accgacaaag
     3961 cggttctcca gggtcggcac ttcgccgccc ggcagcgggg tgtaatcggc acgctgcggc
     4021 ttgccgcgcc cgcggccgaa ggcaccggat ttgagcgaca gcagcgccag accgccccgg
     4081 ccgccgcgca ccggctccca catcgcgtcg ccgccatagc cgctgcggac cagcgcctgg
     4141 aaattgccgt ccgcgtccga tcgcatcccg aactggttga ccggcccgcc ccagaccacc
     4201 gcggcctggg gggcgctgcc gtcgagcggc atccggtaaa gcagcccgcg cggatcgggc
     4261 gggctctggt cccgccacga gcgcagggtc agattgccgg tccagacata gaccgcgtcg
     4321 ggcccgacat agaaggtccg gctgtaggag ccgagcacga cctgcgcctt gcagtccatc
     4381 accggggcgg tgaggttgca ggtggtgacc gcgtgatagg tggtcaggtt ggcccgcttc
     4441 aggttcagca gcagatcggg cacgtggatg tcgcgggcca gggcaatgcg ggtgaaaccg
     4501 cccttcgcgc cattttcggc cgaccagcgc cgcatccccg gcaggctgcc cagcggatcg
     4561 cgcatgttga tcggcagcgg cgcgtaaacc accagccggt tgcccaccag ccgcgaggca
     4621 tagttctcgg ccgagtaata gtcgtccgaa cgcaggtgat aggtatcgac cagggtgaag
     4681 cggcccagcc ggtccagctt gaagcggttg atctccgtcc cgccgcgcgc atagttgtag
     4741 ccgatcacga tcacccagtc gcccgagacc agcatctcgt cataccaggc cccgccccgc
     4801 ccgttgaacg gcggaaaggc atcgatcgtg tcgatcagct tcaggccgcc ggcttcggta
     4861 tcgatgctga acagccgccc gcggcgcagc acgatcaggt tgcggccgcg ctgcttgacg
     4921 atcccgccct cgtcgatccc ctgggtctgg acattggtga tgccctccgc gccgggagca
     4981 ggcggcggcg cggcgcggcc ggagacgacc atcggtgcgg gggcggccat cggtggcggg
     5041 gccggtgggg ccggaacggg aggcggaccg cccgcttcgg ccatcagccc gtcctcgacc
     5101 attacggtcc ggcggatcag ccgcttcagc tgggcatcgc tgccgaccga gggcatcgcc
     5161 tcgcgcttgg ccagggccgg ctccgcggga acaaacccga tcagcgccgc gaccaacacc
     5221 acacccatcc agaaccgcgc catggacccc tccttcag
//
LOCUS       DASUDV010000040         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_45497,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000040 DASUDV010000000
VERSION     DASUDV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45497"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..>2406
                     /gene="ppdK"
                     /locus_tag="VFV30_00835"
     CDS             293..>2406
                     /gene="ppdK"
                     /locus_tag="VFV30_00835"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX4846661.1"
                     /translation="MNKQVFTFGGTVRDTDPRSRDKTIVGGKGANLAEMAGIGLPVPP
                     GFTITTDECVRYLAEGGDFSDSVKAEVAAALGHIETAVGKKFGDPADPLLVSVRSGAR
                     VSMPGMMDTVLNLGLNDITVAGLAASSGDARFAWDSYRRFIQMYSDVVLGIDHHLFED
                     ALEIAKEDNGFYADVEMEAEHWQALVAEYLDIVQDTLGQSFPQDPTEQLWGAIRAVFD
                     SWDSDRAKVYRRLNDIPGDWGTAVNVQAMVFGNMGDTSATGVAFTRDPATGEKAYYGE
                     WLVNAQGEDVVAGIRTPQYLTKASRERAGAKPLSMEEAMPAAYAELAAVFELLEKHYR
                     DMQDIEFTVERGKLWMLQTRSGKRTAKAALKMAVDMAGEGLIDEKTAICRVDPMALDQ
                     LLHPTLDPDAPRDVLTTGLPASPGAASGAIVLDADTAEKRAAMGDAVILVRVETSPED
                     IHGMHAAKGILTARGGMTSHAAVVARGMGRPCVSGASAVSIDMAGRTLRIGTRELKEG
                     DRITLDGTTGEVMFGHVPTIEPELVGDFGVLMSWADKHRRMKVRTNAETPDDCRTARQ
                     FGAEGVGLCRTEHMFFDHSRISAVRQMILAEDEAGRRAALAKLLPEQRADFVSIFEVM
                     AGLPVTIRLLDPPLHEFLPHADAEFAELSDMIGIGADTLKRRAAELHEFNPMLGHRGC
                     RLGITFPEIYEMQARAIFEAAC"
BASE COUNT          425 a          771 c          831 g          379 t
ORIGIN      
        1 ccgatcgatg ccttcttcga gcaggtcact gtcaatgatg ccgataatga gaaacgccga
       61 taccgcctcg cgctgctggc gcggctgcgc gacgcggtgc atggcgtggc cgacttctcg
      121 cggatcgagg gctagggcag caggcggagc gcggtttttt gtgccccggg ctgcgcaggc
      181 ccggaccaaa cgggcgaaaa ccggcggaaa tccgcgctgc ggtggggtgt tggaacaagc
      241 ggacacagtg tcaactgtgt caacctaacg gttcaggaaa gtggcagggc ggatgaacaa
      301 gcaggtcttc accttcggcg gcaccgtccg cgacaccgat ccgcgcagcc gcgacaagac
      361 gattgtcggc ggcaagggcg ccaacctggc cgaaatggcc gggatcggac tgccggtgcc
      421 cccgggcttc accatcacca ccgatgaatg cgtgcgctat ctggccgaag gcggcgactt
      481 ctcggacagc gtgaaggccg aagttgccgc cgcgctgggc cacattgaaa cggccgtggg
      541 caagaagttc ggtgatccgg ctgatccgct gctggtctcg gtccgctctg gcgcgcgggt
      601 ttcgatgccg gggatgatgg acacggtgct caacctgggc ctgaacgaca tcaccgtggc
      661 agggctggcg gcctcgtccg gcgatgcccg gttcgcctgg gattcctacc gccgctttat
      721 ccagatgtat tccgacgtgg tgcttggcat cgatcaccac ctgttcgagg acgcgctgga
      781 gatcgccaag gaagacaacg gcttctacgc cgatgtagag atggaggctg aacactggca
      841 ggcgctggtg gccgagtatc tcgacattgt tcaggatacg ttgggccagt cgttcccgca
      901 ggacccgacc gagcagctgt ggggcgcgat ccgcgcggtg tttgacagct gggattcgga
      961 ccgggccaag gtctaccgcc gattgaacga cattccgggg gactggggca cggcggtcaa
     1021 cgtccaggcc atggtcttcg gcaacatggg cgataccagc gccaccggcg tggcctttac
     1081 ccgcgatccg gcgaccggcg agaaggccta ttacggcgaa tggctggtca acgcccaggg
     1141 cgaggacgtg gtcgccggga tccgcacccc gcagtacctg accaaggcca gtcgcgaacg
     1201 cgccggggcc aagccgctct ccatggaaga ggcgatgccc gcggcctatg ccgagcttgc
     1261 cgccgtgttc gagctgctgg aaaagcacta ccgcgacatg caggacatcg aattcacggt
     1321 ggagcgcggc aagctgtgga tgctccagac ccgcagcggc aagcgcaccg ccaaggccgc
     1381 gctgaagatg gcggtcgaca tggccggcga aggcctgatc gacgagaaga ccgcgatttg
     1441 ccgtgtcgat cccatggcgc tggaccagtt gctccacccg acgctcgatc ccgatgcccc
     1501 gcgcgacgtg ctgaccaccg gcctgccggc cagcccgggg gcggcctccg gcgcgatcgt
     1561 gcttgatgcc gataccgccg aaaagcgcgc ggcgatgggc gatgcggtga tcctggtgcg
     1621 ggtggaaacc agcccggaag acattcacgg catgcacgcc gccaagggca tcctgaccgc
     1681 gcgcggcggg atgaccagcc acgccgccgt ggtggcacgc ggcatgggcc ggccctgcgt
     1741 ttcgggcgcc tcggcggttt cgatcgacat ggccgggcgg accctgcgga tcggcacgcg
     1801 ggaactgaag gaaggcgacc ggatcacgct ggacgggacc accggcgaag tgatgttcgg
     1861 ccacgtcccg acgatcgagc ccgagctggt cggcgatttc ggcgtgctga tgagctgggc
     1921 cgacaagcac cgccggatga aggtgcggac caacgccgaa acgcccgacg attgccgcac
     1981 cgcgcgccag ttcggcgcgg aaggcgtggg cctgtgccgg accgagcaca tgttctttga
     2041 ccattcgcgg atttcggccg tgcggcagat gatcctggcc gaggacgagg ccggccgccg
     2101 cgccgccctg gccaagctgc tccccgagca gcgcgccgat tttgtcagca ttttcgaagt
     2161 gatggcgggc ctgccggtaa cgatccgctt gctcgatccg ccgctccacg aattcctgcc
     2221 ccacgccgac gccgaatttg ccgagctgtc cgacatgatc gggatcggcg ccgacacgct
     2281 gaagcgccgc gcggcggagc tccacgaatt caacccgatg ctgggccatc gtggctgccg
     2341 cctggggatc accttcccgg aaatctacga gatgcaggcc cgcgcgatct tcgaggcggc
     2401 ctgcga
//
LOCUS       DASUDV010000041         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_332772,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000041 DASUDV010000000
VERSION     DASUDV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332772"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFV30_00840"
     CDS             <1..990
                     /locus_tag="VFV30_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4846662.1"
                     /translation="WWAVMLFVFVAGLELDLGEAWKWRRETGLTAGLALGVPLLFGAG
                     AALLLLEFSDPAGWIGAKGQTWQFVAGVGMACAVTALPILILFMQKLEILRTPLGQRI
                     LRYASLDDILIWAVLALVLLDWERVGRQAAYLAGFAVAVWLVRKLIARVPERDRWYIA
                     LVWLAANGYLADWSGLHYMVGAFLAGAALDARWFGTEAIDRFRDTVLLAFMPVFFLST
                     GLRTSWDMGGIAAFAAAALLLTAMVTGKLAGVALAGRLLGWAKGEAWIIGWLLQTKAL
                     IMIIFVNVLFDKAIITSGAFTALLLAAVGSTMLTIPLVSPALKKLDELKAKGG"
     gene            complement(1140..2348)
                     /locus_tag="VFV30_00845"
     CDS             complement(1140..2348)
                     /locus_tag="VFV30_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4846663.1"
                     /translation="MKRGAGQNRDVTRHWRPATRAVRGGTWRSEQGETSEALFLTSGY
                     AYDDAGTAAARFAGEAEGMTYSRLQNPTVAMLEERIALMEGAEACRAQASGMAAMTAA
                     LLCQLSAGDHLVAARAAFGSCRWLVDSLCPRFGIEATVIDSRDNAAWEKAIRPNTKVF
                     FFETPANPTMDIVDLEYVCGLAKARGITTVVDNAFATPALQRPIEWGADVVAYSATKL
                     MDGQGRVLAGAVSGSADFINNVLLPFQRNTGPNLSPFNAWVVLKGLETLDLRIRRQSE
                     NALKVGQFLEGRVPRINHPGLASHPQHALAMKQMDAAGSIFSFEVDGGREQAHALLDA
                     LQLIDISNNIGDSRSLMCHNASTTHYSVSAEVRAEMGVGEGMLRLNVGLEDPDDVIED
                     LDQALKAAGL"
BASE COUNT          328 a          810 c          826 g          409 t
ORIGIN      
        1 tggtgggcgg tgatgctgtt cgtcttcgtc gccgggctgg aactggacct gggcgaggcg
       61 tggaaatggc gccgcgaaac cgggctgacc gcggggctgg cgctgggcgt gccgctgctg
      121 ttcggggccg gcgcggcgct gctgctgctg gagttcagcg atcccgccgg ctggatcggg
      181 gccaaggggc agacctggca attcgtcgcc ggggtcggca tggcctgcgc ggtcacggcc
      241 ctgccgatcc tgatcctgtt catgcagaag ctcgagatcc tgcgcacccc gctgggccag
      301 cggatcctgc gctatgccag ccttgacgac atcctgatct gggcggtgct ggcgctggtc
      361 ctgctcgatt gggaacgggt cggccggcaa gcggcttatc tcgccgggtt tgccgtggcg
      421 gtctggctgg tgcgcaagct gatcgcccgc gtgccggagc gggaccgctg gtacatcgcg
      481 ctcgtctggc tggcggccaa cggctacctc gccgactggt cgggcctgca ctacatggtc
      541 ggcgccttcc tcgccggggc ggcgctcgac gcgcgctggt tcgggaccga ggcgatcgac
      601 cggttccgcg acaccgtgct gctggccttc atgccggtgt tcttcctctc caccggcctg
      661 cgcaccagct gggacatggg cgggatcgcc gccttcgccg ccgcggcgct gctgctgacg
      721 gcaatggtta ccggcaagct ggccggcgtg gcgctggccg gccgcctgct cggctgggcc
      781 aagggcgagg cctggatcat cggctggctg ctgcagacca aggccctgat catgatcatc
      841 ttcgtcaacg tgctgttcga caaggcgatc atcaccagcg gcgccttcac cgcgctgctg
      901 ctcgccgccg tgggcagcac gatgctgacg atcccgctgg tcagcccggc gctgaagaaa
      961 ctggacgagc tgaaggcgaa gggcggatag atcgatcctc cctgtgagcg aagctcatgg
     1021 ggaggtggca gcccgcaggg ctgacggagg ggcatgggcg cagcggccgc ggccccaaca
     1081 ccacgcctgc ggcgcggtcc ccctccccat cggctgcgcc gacagggagg atccggcggt
     1141 taaagcccgg ccgccttcag cgcctggtcc agatcctcga tcacgtcgtc cggatcctcc
     1201 agcccgacgt tgagccgcag catgccctcg cccacgccca tttcggcgcg cacttcggcg
     1261 ctgaccgaat agtgggtggt gctggcgttg tggcacatca ggctgcgcga atccccgatg
     1321 ttgttggaga tgtcgatcag ctgcagcgca tcgagcagcg cgtgagcctg ctcgcgccca
     1381 ccgtccactt cgaacgagaa gatcgatccg gccgcgtcca tctgcttcat cgccagggcg
     1441 tgctgcgggt ggctggccag ccccggatgg ttgatccgcg gcacccggcc ttcgaggaac
     1501 tgcccgacct tgagcgcgtt ttcgctctgc cggcgaatcc gcaggtccag cgtttccagg
     1561 cccttcagca ccacccatgc gttgaacggc gagaggttgg gcccggtgtt gcgctggaac
     1621 ggcagcagca cgttgttgat gaaatcggcg ctgcccgaaa ccgccccggc caggacccgg
     1681 ccctgcccgt ccatcagctt ggttgccgaa taggccacca catcggcgcc ccattcgatc
     1741 ggccgctgca gcgccggcgt ggcaaaggcg ttgtcgacca cggtggtgat cccgcgcgcc
     1801 ttggccaggc cgcagacata ttccagatcg acgatgtcca tcgtcgggtt ggccggggtt
     1861 tcgaagaaga agaccttggt gttgggccgt atcgcctttt cccaagcggc gttgtcgcgg
     1921 ctgtcgatca ccgtcgcctc gatcccgaag cgcgggcaca ggctgtcgac cagccagcgg
     1981 caggagccga acgcggcgcg cgcggcgacg aggtggtcgc ctgccgaaag ctggcaaagc
     2041 agcgccgcgg tcatcgccgc catcccgctg gcctgagcgc ggcaggcctc ggccccttcc
     2101 atcagcgcga tccgctcttc gagcatcgcc acggtcgggt tctgaagccg cgagtaggtc
     2161 atcccctcgg cctcgccggc gaagcgcgcg gcggcggtgc cggcgtcgtc gtaagcataa
     2221 cccgaagtca gaaacagcgc ttcggacgtc tcgccctgct cggagcgcca ggtgccgccg
     2281 cgcaccgcgc gggtcgcggg ccgccagtgg cgggtcacgt cgcggttttg gccggcaccc
     2341 cttttcatgg ctcgtcgcgc gtcatcggca tcg
//
LOCUS       DASUDV010000042         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_465233,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000042 DASUDV010000000
VERSION     DASUDV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465233"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFV30_00850"
     CDS             complement(<1..737)
                     /locus_tag="VFV30_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX4846664.1"
                     /translation="MSLPQVLAAARPIEGGFAATVPTDWMQGRTAYGGFSAALALAAA
                     QRMADDLPPLRSATVNFVGPLAGEVEVRAKVLRRGRNASWISAEVANEAGVGLTATFV
                     FMGPVEASSLHLHDVPMPAYTVPLDQAVPLPERAGPSFAPNFDRRFAAPRSEEKVPEI
                     LWWERVKDTAGLDPMLALILLADALPPGVMPLTGPAPISSMTWLINLLTPLPETDDGW
                     YLLQAKGNYAEKGCSSQDMAIWNTRGEA"
     gene            784..1551
                     /locus_tag="VFV30_00855"
     CDS             784..1551
                     /locus_tag="VFV30_00855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4846665.1"
                     /translation="MTKFIDLSIPITTKVVSDPPVMLPQIQYTDHAETWAQIALFFPG
                     LTKEDLPDGEGWAVETLTLSTHNGTHMDAPWHYHSTTREGAERAATIDEAPLDRFFRP
                     GVKLDFSHLPHGHVVTAAEVEAELARIGHDLQPLDIVLIQSGAVYGTENFTDQGVGLG
                     AEATLWLTERGVEVVGTDAWSWDAPFSHTAKRWAENRDPAIIWEGHKAGRIRPYWQIE
                     KLMNLAALPPHGFMLSCFPVKIEGASAGWIRAVALVD"
     gene            1575..2363
                     /locus_tag="VFV30_00860"
     CDS             1575..2363
                     /locus_tag="VFV30_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4846666.1"
                     /translation="MAEQDPFRIERHGAVTSVSINRPERRNALTAEMLVALRGIAESF
                     RTDSATRAVIFRSDCADFSVGADITGMGGEPPAVAELRRTAENGALMLRAIREIHQPT
                     LCLVRGVATGGGACIPSACDFRFGLPDARVGYGEVKLGINLMWHALPLAIQAVGLARA
                     KRMVMTGDLFDAATMADWGFFDEVLSPEDAEDHVMAQAQRYAALPPVAVQMIKRSANA
                     WAGALDQAVMHADADQWLLATRTDDFREGVAAFREKRAPQFRGD"
     gene            complement(2368..>3312)
                     /locus_tag="VFV30_00865"
     CDS             complement(2368..>3312)
                     /locus_tag="VFV30_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX4846667.1"
                     /translation="LVRYQPPVAGWEDDGEANGRRGLDGIRVDDGVYEGGEVSMFYDP
                     MIAKLITWGKTRDEAADKQIEALDKFEIEGLGHNIDFVSAIMQHPRFRSGELTTGFIA
                     EEYPDGFTGAATSDETKRHLAAIAGFVATARADRARQVDQQLDGELEPPYDWSVTIGG
                     QSFAVSLDEEVMVDGEPVDIGVSYTPGDRLIEAHVEGLTYGVKLEPTRTGLKMTTRGA
                     IHSAQILPARVAHLTKHMIEKVPPDLSKFLICPMPGLLVALHVGEGDKVEAGQPLAVV
                     EAMKMENILRAEKSATVKKVNAKAGDSLAVDAVILELE"
BASE COUNT          517 a         1144 c         1124 g          527 t
ORIGIN      
        1 gcctcgcccc gggtgttcca gatcgccatg tcctggctgg agcagccctt ctcggcatag
       61 ttgcccttgg cctgcagcag gtaccagccg tcgtcggttt cgggcagcgg ggtgaggagg
      121 ttgatcagcc aggtcatcga actgatcggc gccgggccgg tcagcggcat gacgccgggc
      181 ggcagggcat cggccagcag gatcagggcg agcatcggat cgaggcctgc agtgtccttg
      241 acccgttccc accacaggat ctccggcacc ttctcctcgc tgcgcggagc ggcaaagcgg
      301 cggtcgaaat tgggggcaaa gctggggccg gcccgttcgg gcagcggcac ggcctggtcc
      361 agcggcacgg tgtaggccgg catcggcaca tcgtgcaggt gcaggctgct cgcctcgacc
      421 gggcccatga agacgaaggt ggcggtgagg ccgactccgg cctcgttcgc cacttcggcg
      481 ctgatccagc tggcgttgcg cccgcgccgc agcaccttgg cgcggacctc cacctcgccc
      541 gccagcggcc cgacgaagtt gaccgtggcg gaacgcagcg gcggcagatc atccgccatg
      601 cgctgcgccg cggccagggc cagcgcggcg gaaaagccgc cataggcggt gcgcccctgc
      661 atccagtcgg tgggcacggt ggcggcaaat ccgccttcga tcggccgcgc ggcggccagg
      721 acttggggca agctcatgcc agtttccatt gacgtaaacg gaaagcgggt caatccttcg
      781 gccatgacaa agttcatcga cctatcgatc ccgatcacca ccaaggtggt gtccgatccg
      841 cccgtcatgc tgccgcagat ccagtacacc gatcacgccg aaacctgggc ccagatcgcg
      901 ctgttcttcc ccggcctgac caaggaggat ctgcccgatg gcgaaggctg ggcggtggag
      961 acgctgacac tctccaccca caacggcacc cacatggacg cgccgtggca ttaccattcg
     1021 accacccgcg aaggggccga gcgcgccgcg acgatcgacg aggccccgct ggaccggttc
     1081 ttccgccccg gcgtcaagct ggatttcagc cacttgcccc acggccacgt ggtgactgcg
     1141 gccgaggtgg aggcggagct ggcacggatc gggcatgatc tgcaaccgct cgacattgtc
     1201 ctgatccagt ccggcgcggt ctatggcacc gagaacttca ccgatcaggg cgtgggtctg
     1261 ggcgccgagg caacgctgtg gctgaccgag cgcggtgtcg aagtggtcgg caccgatgcc
     1321 tggagctggg acgcgccgtt cagccacact gccaagcgct gggccgaaaa ccgcgatccg
     1381 gcgatcatct gggaaggcca caaggccggc cggatccgcc cctattggca gatcgaaaag
     1441 ctgatgaacc tggccgccct gcccccgcac ggcttcatgc tcagctgctt tccggtgaag
     1501 atcgaagggg ccagcgcggg ctggatccgc gcggtggcgc tggtcgacta ggccgcgcgg
     1561 caaggagcat gaccatggcc gagcaggacc ccttccggat cgagcggcac ggcgcggtca
     1621 ccagcgtttc gatcaaccgg cccgaacgcc gcaacgcgct gaccgcggag atgctggtgg
     1681 cgctgcgcgg gatcgccgaa tccttccgca ctgattccgc cacccgcgca gtgatcttcc
     1741 gcagcgattg cgccgacttc agcgtggggg ccgacatcac gggcatgggc ggtgagcccc
     1801 ctgccgtggc ggagctgcgc cgcacggccg agaacggcgc gctgatgctc cgcgcgatcc
     1861 gcgagatcca ccagccgacc ctgtgcctgg tccgcggcgt ggcgaccggc ggcggcgcct
     1921 gcattccgtc cgcctgcgat ttccggttcg gcctgcccga tgcccgggtc ggctatggcg
     1981 aggtcaagct gggcatcaac ctgatgtggc acgctctgcc gctggcgatc caggcggtcg
     2041 gcctggcgcg cgccaagcgc atggtcatga ccggggacct gttcgacgcg gcgaccatgg
     2101 cggactgggg cttcttcgac gaggtgctga gcccggagga cgcggaagac cacgtgatgg
     2161 cccaggccca gcgctatgcc gcgctgcccc cggtcgcggt gcagatgatc aagcgcagcg
     2221 ccaacgcctg ggccggcgcg ctggaccagg cggtgatgca cgccgatgcg gaccagtggc
     2281 tgctcgccac ccggaccgac gacttccgcg agggcgtggc cgccttccgg gaaaagcgcg
     2341 cgccgcagtt ccgcggcgac tgaaccccta ctccagctcc aggatcaccg cgtccacagc
     2401 caggctgtcg ccagccttgg cgttgacctt cttcaccgtg gcgctctttt cggcgcggag
     2461 gatgttttcc atcttcatcg cctcgaccac ggcgagcggc tggccggcct cgaccttgtc
     2521 gccctcgccc acgtgcagcg ccaccagcag gcccggcatc gggcagatca ggaacttgga
     2581 cagatccggc gggacctttt cgatcatgtg cttggtcagg tgggcaacac gcgccggcag
     2641 gatctgcgcg gaatggatcg cgccgcgggt ggtcatcttc aggccggtgc gggtcggctc
     2701 cagcttcacg ccataggtca gcccctcgac atgggcctcg atcagccggt cgccgggggt
     2761 atagctgacc ccgatgtcga ccggttcgcc atcgaccatc acctcttcgt ccaggctgac
     2821 cgcaaaggac tgcccgccga tcgtgacgga ccagtcatag ggcggctcca gctcgccatc
     2881 gagctgctgg tcgacctgcc gggcgcggtc ggcgcgggcg gtggcaacaa agccggcaat
     2941 ggccgccagg tgccgcttgg tctcgtccga ggtggccgcg ccggtgaagc cgtcggggta
     3001 ctcctcggcg atgaacccgg tggtcagctc gcccgagcgg aagcgcgggt gctgcatgat
     3061 cgcgctgacg aaatcgatgt tgtggcccag cccctcgatc tcgaacttgt cgagcgcctc
     3121 gatctgctta tcggccgcct cgtcgcgggt cttgccccag gtgatcagct tggcgatcat
     3181 cgggtcatag aacatcgaga cttcgccgcc ttcgtagacg ccgtcgtcca cccggatgcc
     3241 gtccaggccg cggcggccgt tggcttcgcc gtcgtcctcc cagcccgcca ccggcggctg
     3301 atagcgcacc ag
//
LOCUS       DASUDV010000043         7939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1481538,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000043 DASUDV010000000
VERSION     DASUDV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481538"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFV30_00870"
     CDS             <1..141
                     /locus_tag="VFV30_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX4846668.1"
                     /translation="TVRDFRELVAQKGYRIEGQWFLSGDRETGPAAASWRAEHAVFLL
                     SR"
     gene            complement(287..1060)
                     /gene="fdhD"
                     /locus_tag="VFV30_00875"
     CDS             complement(287..1060)
                     /gene="fdhD"
                     /locus_tag="VFV30_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX4846669.1"
                     /translation="MTSPSAFGADGTRRAVTRVWVPEVPVALEFNGLSYAVMMATPQD
                     LEDFATGFALNEGLAAAPDEVTDLAVAEVAQGWIVRAQLTGLGIDKLTERVRTRVAES
                     SCGLCGIDNLAEVARPLPPVAPHAGIAPAAIFAALARLRAHQPLGRETGAAHAAAWVD
                     TDGIIGLVREDVGRHNALDKLVGAMARAGRPLSPGFVLSTARCSYEIVEKAVRAGATT
                     LVTISLPTSMAAARAAAAGLSLWVLARDDEVTLVNDASS"
     gene            complement(1057..1437)
                     /locus_tag="VFV30_00880"
     CDS             complement(1057..1437)
                     /locus_tag="VFV30_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4846670.1"
                     /translation="MYSHNMVGSNDLAKAKTFYDATFKAIGGKEAIVDDKGRLIYMHG
                     GGLFIVTAPIDGQPATPGNGCTIGFAMENPAQADAWHAAGVAAGGTAIEDPPGVRAGG
                     FGNLYLAYLRDPDGNKLCALHRMG"
     gene            1580..1984
                     /locus_tag="VFV30_00885"
     CDS             1580..1984
                     /locus_tag="VFV30_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846671.1"
                     /translation="MNRKLAAALAAIALIAPVALSAKAPAADRPDLDAQARCAALFAL
                     VARDQNFKVPGSERYPSMAEGGREFFIETGLRLLNERKMPEAELKPYFVALIDGIEKE
                     HAAAPDPAARRASEMDGCLKMRERVLSSKRAN"
     gene            2109..2900
                     /locus_tag="VFV30_00890"
     CDS             2109..2900
                     /locus_tag="VFV30_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4846672.1"
                     /translation="MAVIAVFSMKGGVGKTTLAVELARRAAMTSQRSTLLWDLDPQGG
                     AGFLLGQGEVQRHRAAALFQREGKPRQLVEPTGLPNLSLLPADPSLRNLPVQLARLGM
                     RKRLATMTAMLRGEYERIILDCPAGLGEVSDQVLTAADLLVVPLPASPLSSRALDMLR
                     KELVQNHHRHPPILPVLSMFDTRRPLHREVREGLAANWPVIPLSTHMEQLAVRHEPLE
                     SYAPKSEAARALRRMWAGIEAKLAETSQAGRKLHGPFSAAQALPA"
     gene            2948..3373
                     /locus_tag="VFV30_00895"
     CDS             2948..3373
                     /locus_tag="VFV30_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846673.1"
                     /translation="MTVSVRVPLAALLAASVAFTAAPAAARPDGDMARELRDPRTQQA
                     MGDLLGALFGAVLNMKAEPLARAMDAAGDREGARRIPRGATIGDLAGPEARALPREIR
                     RRAPALVGALGELAGALEEMAPEFEKIGRDFERSVENRD"
     gene            3412..4305
                     /locus_tag="VFV30_00900"
     CDS             3412..4305
                     /locus_tag="VFV30_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846674.1"
                     /translation="MARSQRHVIVDIGSNTVRLVVFAGNRRAPEELYNDKITASLGRG
                     VVARGKLDARSMAEALSGLKRFAALIRLVAPQSLDVVATAAVRDAANGAEFLDKVRAL
                     GLPARLLSGEEEAQASAWGVIASHPGARGLVADLGGGSLELARVGDDTVFECASFRLG
                     VLPVAAIRAGGRGRLRGTLKEALSAHSWLDQMRGQRLYLVGGAWRALDKARVQLHGGT
                     PRSAFPALEARTLKESVRAAGVRRLAAMPGISSTRAAQLEHAAALLWALVAETGPTDI
                     EVSGAGLREGLLFRGRDEFST"
     gene            4371..5042
                     /locus_tag="VFV30_00905"
     CDS             4371..5042
                     /locus_tag="VFV30_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4846675.1"
                     /translation="MWQLYQFPLCPFSRKVRLLMSEKGLGYELWRENPWEGRDEFLAL
                     NPAGRTPVLHESEKGITLVDSRAICEYFEETVDKNPMINGTAANRAEIRRLVALFDEN
                     FYGDVTAPLLHERMKKRLVYRQSPDSRVLREAMKLAHEHLYYIDHLIDHRPWLAGASM
                     SLADLAAAAQISVADYLGGLDWSGHEQAASWYAVFKSRPSFRPLLAERMEVIQPPSHY
                     ADVNA"
     gene            5079..5444
                     /locus_tag="VFV30_00910"
     CDS             5079..5444
                     /locus_tag="VFV30_00910"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX4846676.1"
                     /translation="MAPLLAFALIVAVGLTALIVWRGRHYGRLAREGVKITATVERKF
                     RTGAGGPGSRGRRIAFSYTGPDGQAYRRAATVTLSKWQELEEGYPIEIYVLPDRPGIS
                     GPVWLVDAAREALRKAGRL"
     gene            complement(5441..6085)
                     /locus_tag="VFV30_00915"
     CDS             complement(5441..6085)
                     /locus_tag="VFV30_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846677.1"
                     /translation="MRRYDLAIHGSGLLPGLLAIHLLGCDPGQALLLAFTDAEPCGDK
                     LEPVLEASLSAPARALVDPFIVREWPRFLVTRQGETTEHPGTVLLLDPVQVWLELRGL
                     LPDAAMPAACTDLQWAAPVLSWDDHRVEAGAYVDLSVFASDGCSEIVGGTGGTGLPLP
                     VMADFDVGDEPWDAFQHLPLGDDRIYIRKRRCRGPVEDALIAGFGRALSDLMGR"
     gene            6578..7189
                     /locus_tag="VFV30_00920"
     CDS             6578..7189
                     /locus_tag="VFV30_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846678.1"
                     /translation="MAASAMACAQPALAQVSQEQYAYYRDPALPAYPYPGAASATLSV
                     PVTASVGGVCGFRTGNAPNATLTYPNIDTTAWSGQVPFIAECTAPWRIAVSSQNGALR
                     NTATVPTGYQNRAPYTVSLNVASDAGTVTAACPVAQIDQALGSSTCNFKGTASTSNGL
                     LIGRSFGLSGSYIQVAAPAYAGPDVLVSGNYTDTLVVTISPAS"
     gene            7279..7860
                     /locus_tag="VFV30_00925"
     CDS             7279..7860
                     /locus_tag="VFV30_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846679.1"
                     /translation="MKKIALLAITAAAAIATPAMAQSTTGTIIINGTVGNKCTVLTGT
                     GTAATWGGTVNLGELSQADGTLRSSSSLSTDFAAAGSGVLQARIVCTSANPSITVNAD
                     PLVNTAPVTAGQGYTNTVHFQADVAVTKVSGSSTFSNDSNAATTGPTAVGDRLAATGN
                     NVSVSTTNWRTVGTDTQLVSGNYQGQIVVTIAP"
BASE COUNT         1266 a         2713 c         2737 g         1223 t
ORIGIN      
        1 accgtgcgcg atttccgcga gctggtggcg cagaagggct accggatcga agggcagtgg
       61 ttcctctcgg gcgaccgcga aaccggcccg gcggcggcca gctggcgcgc cgagcacgcg
      121 gtgtttttgc tttcgcgata ggatcctccc tgtccgcgca gcggatgggg agggggaccg
      181 ccaccgcagg cgggggtggt ggggccttcc ggcaccagcg ccatggcccc tccgtcagcg
      241 cttcgcgctg ccgcctcccc attcggcttc gccaaacagg tagggtctag ctggaggcgt
      301 cgttcaccag cgtcacttcg tcgtcccgcg ccagcaccca caggctgagc ccggccgccg
      361 ctgcccgcgc ggccgccatg gaggttggca gcgagatggt gaccagcgtg gtcgccccgg
      421 cacggacggc cttttcgacg atttcatagc tgcagcgcgc ggttgagagg acaaagccgg
      481 ggctgagcgg ccggccggcc cgggccatgg ccccgaccag cttgtcgagc gcgttgtgcc
      541 ggccgacatc ctcgcgcacc aggccgatga tcccgtccgt gtccacccag gccgcggcat
      601 gggccgcgcc ggtttcgcgg cccagcggct ggtgcgcgcg cagcctcgcc agcgcggcaa
      661 agatcgcggc gggggcaatt ccggcatggg gggcgaccgg gggcaggggg cgggccactt
      721 cggccaggtt gtcgatcccg cacaggccgc agctcgattc cgccacgcgg gtgcggaccc
      781 gttcggtcag cttgtcgatc cccaggcccg tcagctgcgc gcggacgatc cagccctggg
      841 cgacttcggc gacagcaagg tcggtcactt catccggagc ggcggccagc ccttcgttca
      901 gggcaaagcc ggtcgcaaag tcttcaaggt cctgcggcgt ggccatcatc accgcatagg
      961 acaggccgtt gaactccagc gccaccggca cctccggcac ccagacgcgc gttaccgcac
     1021 ggcgggtgcc gtcggcgccg aaggcgctgg ggctggtcat cccatccggt gcagggcgca
     1081 gagcttgttg ccgtccggat cgcgcaggta ggccagatag aggttgccga acccgcccgc
     1141 gcggacgccc ggcggatctt cgatcgcggt gccaccggcg gccacgccag cggcgtgcca
     1201 ggcatcggcc tgggccggat tttccatggc gaagccgatc gtgcagccgt tgcccggggt
     1261 ggccggctgg ccatcgatcg gggcggtgac gatgaacagc ccgccgccgt gcatgtagat
     1321 caggcggccc ttgtcatcga cgatcgcctc tttcccgcca atcgccttga acgtggcgtc
     1381 gtagaaggtc ttcgctttgg ccagatcgtt cgatccgacc atgttgtggc tgtacatgcc
     1441 gcctctcctt ttcagttgcg attcgctacg tgtatcgctt cgaggcgcgg ggtcaatcgc
     1501 gcgccgccgc tggatcgcag aaccgggcgg gcggcgcaat gaaagttgcg gttcagcttg
     1561 gacacgctag gcaggcagaa tgaaccgcaa gcttgcggcc gcgctggcgg ccatcgccct
     1621 gatcgccccc gtggcgcttt ccgccaaggc cccggccgcc gaccggccgg acctggatgc
     1681 ccaggcccgc tgcgccgcgc tgtttgcgct ggtggcgcgg gatcagaact tcaaggtgcc
     1741 cgggtcggag cgctatccct cgatggccga aggcgggcgc gagttcttta ttgagaccgg
     1801 cctgcgcctg ctgaacgaac gcaagatgcc cgaggccgag ctgaagccct atttcgtcgc
     1861 gctgatcgac gggatcgaaa aggaacacgc ggccgcaccc gatccggcgg cccggagggc
     1921 gtcggagatg gacggatgtc tgaaaatgcg ggagcgcgtt ctttcgtcga agcgcgcgaa
     1981 ttagggccaa aaccctaaac gcttcggccc gggactgtcc tgcttcgggc ataagctaaa
     2041 gtgcagattg ccaatcggcg caaatccggc attctccgcg ctgaaaagca aggggattcg
     2101 gtgtcgcaat ggcggtcata gccgtcttca gcatgaaagg gggcgtcggc aaaaccaccc
     2161 tggcggtgga gctggcgcgg cgcgcggcga tgacgtcgca gcgcagcacc ctgctgtggg
     2221 acctcgatcc gcaaggcggc gccggctttc tgcttggcca gggcgaggtg cagcgccacc
     2281 gcgcggcggc cctgttccag cgcgaaggca agccgcgcca gctggtcgag ccgaccggcc
     2341 tgcccaacct ctcgctgctg ccggccgatc cctcgctgcg caacctgccg gtccagctgg
     2401 cccggctggg catgcgcaag cggctggcca cgatgaccgc gatgctgcgg ggcgagtatg
     2461 agcggatcat cctcgattgc ccggccgggc tgggggaagt gtccgaccag gtcctgaccg
     2521 cggccgatct gctggtggtg ccgctgccgg cctcgccgct ctcgtcgcgg gcgctggaca
     2581 tgctgcgcaa ggagctggtc cagaaccacc accgccatcc tccgatcctg ccggtcctgt
     2641 cgatgttcga cacccgccgc ccgctgcacc gcgaagtgcg cgaagggctg gccgcgaact
     2701 ggccggtgat cccgctgtcc acccacatgg agcagctggc cgtgcggcac gagccgctgg
     2761 aaagctatgc gcccaagagc gaggcggccc gggcgctgcg gcggatgtgg gccgggatcg
     2821 aggcgaagct ggccgagacc agccaggctg gacgcaagct gcacggtccg ttcagcgcgg
     2881 cgcaagcgct tccggcctag aggctccaac cgcgcccgct cttcgggcgc gccaggggtt
     2941 cgctgccatg actgtttccg tccgtgttcc gcttgcggcg ctgcttgccg cgtccgttgc
     3001 tttcaccgcc gcgccggccg ccgcccgccc cgatggcgac atggcgcgcg agctgcgcga
     3061 tccgcgcacc caacaggcga tgggcgatct gctcggcgcg ctgttcggtg cggtgctcaa
     3121 catgaaggcc gagccgctgg cccgggcgat ggacgccgcc ggggaccgcg aaggggcccg
     3181 ccgcatcccg cgcggggcga cgatcggcga tctggccgga cccgaggccc gcgccctgcc
     3241 gcgcgaaatc cgccgccgcg ccccggcgct ggtcggcgcg ctcggcgaac tggccggcgc
     3301 gctggaagaa atggcgcccg aattcgagaa gatcggccgc gatttcgaac ggagcgtgga
     3361 aaaccgcgac taggcctagc gccggtcctg cccgctccct accaagcggg catggcccgg
     3421 tcgcaacgtc acgtcatcgt cgatatcggt tcgaacacgg tccgcctggt cgtgttcgcc
     3481 ggcaaccggc gcgcgcccga agagctctat aacgacaaga tcaccgccag cctcggccgc
     3541 ggggtggtgg cgcgcggcaa gctcgacgcg cgcagcatgg ccgaggcgct gtccggcctg
     3601 aagcggttcg ccgcgctgat ccgcctggtt gccccgcaaa gcctggacgt ggtcgccacc
     3661 gccgcggtgc gcgatgccgc caacggcgcc gaattcctcg acaaggtgcg cgcgctgggc
     3721 ctgccggcgc ggctgctttc gggcgaggag gaggcccagg cctcggcctg gggcgtgatt
     3781 gccagccatc cgggtgcgcg ggggctggtg gccgatctgg gcgggggcag cctggaactg
     3841 gcccgggttg gcgatgacac ggtgttcgaa tgcgcctcgt tccggctcgg cgtcctgccg
     3901 gtggcggcga tccgcgccgg cgggcgcggg cggctgcgcg ggacgctgaa ggaggcgctg
     3961 tccgcccaca gctggctgga ccagatgcgc ggccagcggc tctacctggt tggcggggcc
     4021 tggcgcgcgc tcgacaaggc ccgggtccag ctccacggcg gaaccccgcg cagcgccttt
     4081 ccggcgctgg aggcgcggac cctgaaggaa agcgtccgcg ccgccggcgt gcggcggctg
     4141 gccgccatgc ccgggatcag cagcacccgt gccgcgcagc tggaacacgc cgcggcgctg
     4201 ctgtgggcgc tggtggccga aaccgggccg accgatatcg aagtgtcggg cgcggggctg
     4261 cgcgaaggcc tgctgttccg cggccgggac gagttttcca cctgaaatcg cgtctcccgt
     4321 gccgccgcgt gggttgaagc gcgaatcgcg ctccggcatg ggggactgtc atgtggcagt
     4381 tgtaccagtt ccccctgtgc ccgttcagcc gcaaggtccg tctcctgatg agcgagaagg
     4441 gccttggcta tgagctgtgg cgcgaaaacc cgtgggaagg gcgcgacgag tttctggcgc
     4501 tcaacccggc cggccgcacg ccggttctcc acgaatccga gaaaggcatc accctggtcg
     4561 atagccgggc gatctgcgaa tatttcgagg aaacggtcga caagaacccg atgatcaacg
     4621 gcaccgcggc caaccgggcc gagatccgcc gcctggtcgc gctgttcgac gagaacttct
     4681 atggcgatgt caccgcgccg ctgctgcacg agcggatgaa gaagcggctg gtctatcgcc
     4741 agtcgccgga ttcgcgggtc ctgcgcgagg cgatgaagct ggcgcacgag catctctatt
     4801 acatcgatca tctgatcgat caccgcccgt ggctggccgg ggcctcgatg agcctggccg
     4861 atctggccgc cgcggcgcag atttcggtgg ccgattacct tggcgggctg gactggtcgg
     4921 gccacgaaca ggcggccagc tggtacgccg tgttcaagag ccggccgagc ttccgcccgc
     4981 tgctggcgga acggatggaa gtgatccagc cgcccagcca ctacgccgac gtcaacgcct
     5041 gattgccagg ccctgcggct gggctaaact gcgcggcgat ggccccgctg cttgcctttg
     5101 ccctgattgt cgccgtgggc ctcaccgccc tgatcgtctg gcgcggccgc cactatggcc
     5161 ggctggcgcg ggaaggggtg aagatcaccg ccacggttga gcgcaagttc cgcaccggcg
     5221 ccggcgggcc gggttcgcgc gggcggcgga tcgccttttc ctacaccggc ccggacgggc
     5281 aggcctatcg ccgcgcggcg actgtcaccc tgtcgaaatg gcaggaactg gaagagggct
     5341 atccgatcga gatctatgtc ctgcccgacc ggccggggat cagcggcccg gtctggctgg
     5401 tcgacgccgc gcgcgaggcc ttgcgcaagg ccggccggct ctagcgcccc atcagatcgc
     5461 tcagcgcgcg gccgaacccg gcgatcagcg cgtcctccac cggaccgcgg cagcggcgtt
     5521 tgcggatgta gatccggtca tcgcccagcg gcaggtgctg gaacgcgtcc cacggttcat
     5581 cgccgacatc gaaatcggcc atgaccggca gcggcaggcc ggtcccgccg gttccgccga
     5641 caatctcgct gcagccgtcg ctggcaaaga ccgacaggtc gacataggcg cccgcctcga
     5701 ccctgtggtc atcccagctc agcaccgggg cggcccattg caggtcggtg caggccgccg
     5761 gcatggccgc gtcgggcagc aggcccctca gctccagcca gacctggacc gggtcgagca
     5821 gcaggacggt gccgggatgt tcggtggttt cgccctgccg ggtgaccagg aaccgcggcc
     5881 attcgcgtac gatgaaggga tcaaccaggg cccgggccgg ggcggacagg cttgcctcca
     5941 gcaccggctc cagcttgtcg ccgcagggct ccgcatcggt gaaggccagc agcagcgcct
     6001 ggccgggatc gcaaccgagc agatggatcg ccagcaggcc cggaagcagg ccggaaccgt
     6061 ggatcgcaag gtcgtagcgg cgcattggca acaccgttcc acagcccgga tcagagggca
     6121 agggcattgc aagaaaacgg gaaaccgccg ggggcccttg gcgcgactcg agtcgcgggc
     6181 cgagtcgcgt gatcgaaatg ccacagaggc cccgttaagc tttggcgtta accggattta
     6241 tcaaggttgc gacaagttgg acgaatgcga ctcggccaaa aaaacaggct ttgaatcagc
     6301 ctgttataga gagctaaatg gcggaaatcc ttgtcaatga tggttaacac catgggcgaa
     6361 aaagaatcca cttggcaagc accccccaga tggcgttaac ctgacagggc aacaagtgag
     6421 cacctggacg agggtccggg gaaagctcac gaccattggg ccgcatcgat aattttctga
     6481 ctccccgtcc ggaatccttc cggccgtgtg agtagacgcg tggagtgtgg cgatgttgcc
     6541 tgttgggaaa tgtgcggaac ggatggcgtg ggggctgatg gcagcttcgg ccatggcctg
     6601 tgcgcagccg gctctggccc aggtttcgca ggaacagtac gcctattacc gcgatcccgc
     6661 gctgccggcc tatccctatc cgggtgcggc ttcggccacc ctttcggttc ccgtgacggc
     6721 ttcggtcggc ggggtttgcg ggttccgcac cggcaatgcg ccgaacgcga ccctgaccta
     6781 tccgaacatc gacaccaccg cctggagcgg ccaggtgccc ttcatcgccg agtgcaccgc
     6841 cccgtggcgg atcgcggtct cgtcacagaa cggcgcgctg cgcaacaccg caacggtgcc
     6901 gaccggctat cagaaccgcg cgccctacac cgtgtcgctc aacgtcgcct cggacgccgg
     6961 gacggttacc gccgcctgtc cggtggcgca gatcgaccag gcgctgggca gctcgacctg
     7021 caatttcaag ggcaccgcca gcaccagcaa cggcctgctg atcgggcgct cgttcgggct
     7081 tagcggcagc tacatccagg ttgccgcgcc ggcctatgcc gggccggacg tgctggtttc
     7141 gggcaactac accgacactc tggtcgtgac gatttcaccc gcctcttgag aagcgggaat
     7201 acgggtaccc cgggcggatg gtccaaacgg ggaaatgcaa gaaccgccgg gaaaccggca
     7261 caatgcgagg acgaagacat gaagaagatt gctctgcttg cgatcactgc cgcagcggcg
     7321 attgcgaccc cggcgatggc ccagtccacc accggcacca tcatcatcaa cggcaccgtt
     7381 ggcaacaagt gcaccgtgct gaccggcacc ggcaccgcgg ccacctgggg cggcaccgtg
     7441 aacctcggtg aactgtcgca ggccgacggc accctgcgct cgagctcgag cctgtcgacc
     7501 gactttgccg ctgccggcag cggcgtgctg caggcccgca tcgtctgcac cagcgccaac
     7561 ccgtcgatca ccgtcaacgc cgatccgctg gtcaacaccg ctccggtgac cgccggccag
     7621 ggttacacca acaccgtcca cttccaggcc gatgtcgcgg tgaccaaggt ttcgggttcg
     7681 agcaccttca gcaacgactc gaacgccgcc accaccggcc cgaccgctgt gggtgaccgc
     7741 ctggccgcca ccggcaacaa cgtctcggtg agcaccacca actggcgcac cgtcggcacc
     7801 gacacccagc tggtctcggg caactaccag ggccagatcg tcgtcacgat cgccccgtaa
     7861 gggacgaccg cgcgaaagga ctgaatgatg cgtaagcttc tcatccttgc cgttcccgcc
     7921 gctctggccg ccagccccg
//
LOCUS       DASUDV010000044         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_796750,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000044 DASUDV010000000
VERSION     DASUDV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796750"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFV30_00930"
     CDS             complement(<1..446)
                     /locus_tag="VFV30_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846680.1"
                     /translation="MILWAAVAGALIGWGLAGGGVAGLVFGAIFGAGMGAWLRSVLKA
                     EVAREVHLHLAGLNLSPAAPEEAEAPLEAWPEPATAPPAPEPVAEPARRPEPADALAQ
                     AWPETPPAPAVTAPAATYRDEPNLAEQLIQRTVEWFTGGNTIIRVGL"
     gene            complement(522..1301)
                     /locus_tag="VFV30_00935"
     CDS             complement(522..1301)
                     /locus_tag="VFV30_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4846681.1"
                     /translation="MPRLQGKLCIVTGAARGIGLAISAAFQREGASVILTDRDAENGA
                     ATAERLGCRFLTLDVAREDDWAALAAAVPQADVLVNNAGITGFEDSPGPHDPEHASLA
                     DWRVVHAVNLDGTFLGCRYAIGAMKAKGAGSIINISSRSGLVGIPGAAAYASSKAGSR
                     NHTKSVALYCAQNGWAIRCNSIHPAAILTPMWEPMLGDGPDRAARMAALVADTPLKRF
                     GEADEVAALAVLLASDEAAYITGAELNIDGGLLAGSAATPG"
     gene            complement(1464..2333)
                     /locus_tag="VFV30_00940"
     CDS             complement(1464..2333)
                     /locus_tag="VFV30_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846682.1"
                     /translation="MQAGPAPIEADPPTPLSQVPAKPLVFGAIGLVGLLLAAWLFYEL
                     PKQGAADSAPAATEAAFDPNAPRPQEWFDNYKDSFVSAEVERLVTGPAQKRNFPTARG
                     SQVVGTLAAGTLVSGRFVQGGDGDTKWLKTGDGAYVWDGNLAAPESITSLGMNRFQIE
                     VPFSRVMDSFKSEGRYSSSDPMWETSACEIYTSNDGLVYAMVIDGQVTRFETDNPQLA
                     TVNGIRVGSTEAELIRAYGRGKLKKEANPYAGVDYFYWVGNKRGIKFNVDGGKVTMIT
                     AGNESIRFVEGCL"
     gene            complement(2488..>3814)
                     /locus_tag="VFV30_00945"
     CDS             complement(2488..>3814)
                     /locus_tag="VFV30_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4846683.1"
                     /translation="RTLVVNDSETAIATLGTRQGMQLNAIILDQWVPGDEACALIAEL
                     RSRRPALPILMLTTSASPLLAVEAMRAGATDYLIKPVAPDRLMLALRMATTREAPRDE
                     LQPLTEKMPATPDFESMVGAAPTFRAALAVAAKAARGHGAVLIEGESGTGKETVIRAM
                     QAASPRAKAPFRLLNIGGIPSNSVESALFGHEKGAFPGAFDRHIGAIQHADGGTLALD
                     EVDRLPLSVQQRLLESLQRGDVRPIGARHSFRVDVRLIAASNLPLREMTEAGHFLPEL
                     LDLLAPTTVTLPPLRARVGDIPALARHFLARIGDQPGLRPLGITDGALSLLAAYDWPG
                     NVRQLQAVLFRAAVFCDGDALTAEDFPQLSDLLGTDGANQVPLHEGVGVMLYTPDGNL
                     RPLEEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLSELGIDNAA"
BASE COUNT          562 a         1277 c         1358 g          617 t
ORIGIN      
        1 aggccgaccc ggatgatcgt gttgccgccg gtgaaccact ccaccgtgcg ctggatcagc
       61 tgttcggcca ggttgggttc gtcgcggtaa gtggccgccg gtgccgtgac ggcgggggcg
      121 ggcggggttt ccggccaggc ctgggccagc gcgtcggccg gttccgggcg gcgggccggc
      181 tcggccaccg gttcgggtgc gggcggggcg gttgccggtt ccggccacgc ctcaagcggg
      241 gcctcggcct cttccggcgc ggcgggggaa agattgaggc cggcgaggtg caagtgcact
      301 tcccgcgcca cttcggcctt cagcacgctg cgcagccagg cgcccatccc ggcgccgaag
      361 attgcaccaa atacgaggcc ggcgacacca ccgccggcca agccccagcc aatcagcgcc
      421 ccggccactg ctgcccacag gatcatgccc gcctcgcttt cctgccttca atcccgggca
      481 atctggcaca attgcccggg attgccagca aggaggctca attatccggg ggttgcggcc
      541 gatcccgcca gcaggccgcc gtcgatattg agctcggccc cggtgatgta ggcggcctcg
      601 tccgaggcca gcagcacggc cagggcggca acctcgtcgg cctcgccaaa gcgcttcagc
      661 ggcgtgtcgg cgaccagcgc ggccatccgc gcggcgcggt ctgggccatc ccccagcatc
      721 ggctcccaca tcggggtcag gattgcggcg gggtggatcg aattgcagcg gatcgcccag
      781 ccgttctgcg cgcagtaaag cgcgaccgac ttggtgtggt tgcgtgaccc cgccttggaa
      841 ctggcatagg ccgccgcacc gggaatgccg acaaggccgg aacgggacga gatgttgatg
      901 atcgatcccg cgcccttcgc cttcattgcc ccgatcgcat agcggcagcc caggaaggtg
      961 ccgtcgaggt tgaccgcgtg caccacccgc cagtcggcca ggctggcgtg ctccggatcg
     1021 tgcgggccgg ggctgtcctc gaacccggtg atcccggcgt tgttgacgag gacatcggcc
     1081 tggggaacgg ccgcggcgag ggccgcccag tcgtcctcgc gggccacatc gagcgtcaag
     1141 aagcggcagc cgagccgctc tgcggtggca gcgccgttct ccgcgtcacg gtcggtcagg
     1201 atgacgctgg cgccctcgcg ctgaaaggcg gcgctgatcg ccagcccgat tccgcgggcg
     1261 gcgccggtga caatgcacag ttttccctga agtcggggca tggagaattc cttggaaata
     1321 caattggtcc gagcggcccg tcaggggccg cggtgggatt tgcgatccgc cggggcggtg
     1381 tcactggacc aattgaggaa ctccttcgct gcggggcgaa ttgtgccgcg cccggccggc
     1441 aatggcaagc cgctatgccg ggatcacagg cagccttcga cgaagcggat gctctcgttc
     1501 ccggcggtga tcatcgtcac cttgccgccg tcgacgttga acttgatccc gcgcttgttg
     1561 ccgacccagt agaagtaatc gaccccggca taggggttcg cttccttctt cagcttgccc
     1621 cggccatagg cgcggatcag ctcggcctcg gtcgagccga cgcggatgcc gttgacggtg
     1681 gcgagctgcg ggttgtcggt ttcgaaccgg gtgacctggc cgtcgatcac catcgcgtag
     1741 accagcccgt cgttcgaggt gtagatttcg caggcgctgg tttcccacat cggatcgctg
     1801 gagctgtagc gcccctcgct cttgaacgaa tccatcacgc ggctgaacgg cacttcgatc
     1861 tggaaccggt tcatccccag cgaggtgatc gattccggcg cggccaggtt gccgtcccag
     1921 acataggcgc cgtccccggt cttgagccac ttggtgtcgc cgtccccgcc ctggacgaac
     1981 cggccggaaa ccagcgttcc cgcggccagc gtgccgacca cctgcgatcc gcgcgcggtc
     2041 gggaaattgc gcttctgtgc gggtccggtg accagccgct ccacttcggc gctgacgaag
     2101 ctgtccttgt aattgtcgaa ccattcctgc ggacgcggag cgttgggatc gaaggccgcc
     2161 tcggtagcgg ccggcgcgct gtcggccgcg ccctgcttgg gcagttcata gaacagccag
     2221 gcggccagca gcagcccgac gagcccgatt gcgccaaaca ccagcggctt ggccggcacc
     2281 tgcgacagcg gggtgggcgg atcggcctcg atcggcgccg gcccggcttg caacgccggg
     2341 ctgccgcagc gcgggcaaaa ggcctgaccg gatttgagtg cgtttccgca gttgaggcac
     2401 gccgctgaca tcgttgatcc accccctggg attctgtttc ccaaggaatg cgatacggcg
     2461 cgcggtttgt cgaggtgggt cgcagcctca ggccgcgttg tcgatgccca gttcggacag
     2521 cttgcggtac agcgtcgagc ggccgatgcc gaggcggcgg gcgacttcgg tcatccggcc
     2581 gcggtaatga ccgatcgcca ggcggatcac gtcggcctcg atctcttcca gcgggcgcag
     2641 gttgccgtcg ggggtgtaga gcatcacgcc cacgccctcg tgaagcggga cctggttggc
     2701 gccgtccgtg ccgagcaggt cggacagctg cggaaagtct tcagccgtga gggcgtcgcc
     2761 atcgcagaac accgcggcgc ggaacagcac ggcctgcaac tggcgcacgt tgccgggcca
     2821 gtcataggcg gccagcagcg acagcgcgcc gtcggtaatg cccagcgggc gcaggcccgg
     2881 ctgatcgccg atccgggcca ggaagtggcg ggccagggcc ggaatatcgc ccacccgcgc
     2941 gcgcagcggc ggcagggtca ccgtggtcgg agccagcagg tcgagcagtt cgggcaggaa
     3001 atgcccggcc tcggtcattt cgcgcagcgg cagattgctg gcggcgatca gccgcacgtc
     3061 aacccggaag gaatggcgcg caccgatcgg gcggacgtcg ccgcgctgca ggctttccag
     3121 caggcgctgc tggacggaca gcggcagacg gtcgacctcg tcgagcgcca gggtgccgcc
     3181 gtcggcgtgc tggattgcgc cgatgtgccg gtcaaaggcc cccgggaagg cgcccttttc
     3241 gtggccgaac agcgcgcttt ccaccgaatt ggacgggatc ccgccgatgt tgagcagccg
     3301 gaacggcgcc ttggcccgcg ggctggcggc ctgcatggcg cggatcacgg tttccttgcc
     3361 ggtgccgctt tccccctcaa tcaggaccgc accgtggccg cgcgccgcct tggcggcaac
     3421 ggccagagcc gcgcggaagg tgggcgccgc gccgaccatg gattcgaaat ccggggttgc
     3481 cggcatcttt tcggtcagcg gctgcagttc gtcgcgcggg gcctcgcggg tggtggccat
     3541 gcgcagcgcg agcatgagcc ggtcgggcgc gaccggcttg atcaggtaat cggtcgcccc
     3601 ggcgcgcatc gcctcgaccg ccagcagcgg gctggcgctg gtggtcagca tcaggatcgg
     3661 cagggccggg cgccggctgc gcagttcggc gatcagggcg caggcctcgt cacccggaac
     3721 ccactggtcc aggatgatgg cgttgagctg catgccctgg cgggtgccca gcgtggcgat
     3781 ggcggtttcg ctgtcgttga cgaccagcgt gcgc
//
LOCUS       DASUDV010000045         3906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_796777,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000045 DASUDV010000000
VERSION     DASUDV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796777"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /gene="nusA"
                     /locus_tag="VFV30_00950"
     CDS             <1..1360
                     /gene="nusA"
                     /locus_tag="VFV30_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX4846684.1"
                     /translation="LDPDAKLGDFIVDPLPPVDLGRIDAQSAKQVIFQKVRDAERERQ
                     YEEFKDRAGELVTGVIKSVEFGHVIVNLGRAEGVIRRDQQIPREVARVGERVRALILK
                     VERQNRGPQIFLSRAHPDFMKALFAQEVPEIYDGIITIQAAARDPGSRAKIGVISRDS
                     SIDPVGACVGMKGSRVQAVVQEMQGEKIDIIPWSEDTATFVVNALQPATVSRVVIDEE
                     ESRIEVVVPDDQLSLAIGRRGQNVRLASQLTGSQIDIMTEAESSEKRQKEFAERSKMF
                     EEELDVDETLSQLLVAEGFSELEEVAYIELAELATIEGFDEELAEELQSRAQEALDRR
                     EAAYREQRVALGVEDALAEMPHLTEQMLVTLGKAGIKTLDDLADLATDELIAKKRTEQ
                     RRREGSAPPRRSERAEDKGGVLGEYGLSEEQGNEIIMAARAHWFEDEPATEEAANADS
                     SQ"
     gene            1341..2075
                     /locus_tag="VFV30_00955"
     CDS             1341..2075
                     /locus_tag="VFV30_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HEX4846685.1"
                     /translation="MRIPHNEPLGSGTSKQPASGDPERRCILTGDHGSRDDLLRLAIS
                     PDGLVLPDALARAPGRGAWIGVSRADLETALAKGKLKGALARAFKGAELSIPDDLPQR
                     AEAALRRAFTDRLGLEMKSGKLLLGSDRIAENARMGNVAWLAHAADASDDGSRKLDQA
                     WRVGRETEGSGETGLTLPLDRAALSVALGRENVVHLALTDPRSAERVDAPLRRLLRFL
                     GQGHTNEQGRGDSAADPAGPSPVTTN"
     gene            2098..>3906
                     /gene="infB"
                     /locus_tag="VFV30_00960"
     CDS             2098..>3906
                     /gene="infB"
                     /locus_tag="VFV30_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX4846686.1"
                     /translation="MSDSDNKPTLGRKPLGLKKSVEAGQVQQTFSHGRTNKVVVEVKQ
                     RKLIRKPGEAPVAAPPPPPPAPAPAPVAAAPKPAPKPAAPAGETPQERVARLQREAEE
                     ARLAMLEEQTRREQEERLRAQEEEKRRAEQNRKAEADAAAAASAPAAEPAAEPDAAPD
                     AEPEAEAPRGEAPAPRRFTPVAAPKRPEAPAKKIVHPRDKTGGDQRRHAGKLTVSRAL
                     NEDEGARARSLAALKRAREKERRAHFSGQSQPREKQVRDVVVPEAITVQELANRMAEK
                     GADLVKSLFKMGMMVTVNQTIDADTAELLVEEFGHNVQRVSESDVDIDTAEDVDAPET
                     LKARPPVVTIMGHVDHGKTSLLDALRGTDVVRGEAGGITQHIGAYQIKTKGGDLVTFL
                     DTPGHEAFTEMRQRGANVTDIVILVVAGDDGLMPQTIEAINHTKAAGVPMIVAITKSD
                     KPEFNPQKIRERLLEHEVIVEAMSGDVQDVEVSAKTGAGLDNLIEKILLQSELMELKA
                     NPDRPAEATVIEAKLDKGKGPLATVLITRGTLKTGDILVVGTHSGRVRAMLDDKGRQI
                     KVAPPALPVEVLGISGVPMAGDTLTVVESEARAREVA"
BASE COUNT          757 a         1315 c         1342 g          492 t
ORIGIN      
        1 gctcgatccc gatgccaagc tgggcgactt catcgtcgat ccgctgccgc cggtcgacct
       61 gggccgcatc gacgcccagt cggccaagca ggtgatcttc cagaaggtcc gcgatgccga
      121 gcgcgagcgc cagtacgagg agttcaagga ccgcgccggc gaactggtga cgggcgtgat
      181 caagtcggtc gaattcggcc acgtgatcgt caacctgggc cgcgccgaag gcgtgatccg
      241 ccgcgatcag cagatcccgc gcgaagtggc ccgcgtcggc gagcgggtcc gcgcgctgat
      301 tctgaaggtg gagcgccaga accgcggtcc gcagatcttc ctgtcccgcg cccaccccga
      361 tttcatgaag gccctgttcg cgcaggaagt gcctgaaatc tatgacggca tcatcaccat
      421 ccaggccgcc gcccgcgatc cgggcagccg cgccaagatc ggcgtgatca gccgcgacag
      481 cagcatcgac ccggtcggcg cctgcgtcgg catgaagggc agccgcgttc aggccgtcgt
      541 gcaggaaatg cagggtgaaa agatcgacat cattccctgg agcgaagaca ccgcgacttt
      601 cgtggtcaac gcgctgcagc cggccaccgt cagccgcgtg gtgatcgacg aggaagaaag
      661 ccgcatcgaa gtggtggttc ccgacgacca gctgagcctg gcgatcggcc gccgcggcca
      721 aaacgtccgt ctggccagcc agctgaccgg cagccagatc gacatcatga ccgaggcgga
      781 atcgagcgag aagcgccaga aggaattcgc cgagcgttcg aagatgttcg aggaagagct
      841 cgacgtcgat gaaaccctct cgcagctgct ggtggccgaa ggcttcagcg agctggagga
      901 agtggcctat atcgaactgg ccgaactggc gacgatcgaa ggctttgacg aagagctggc
      961 cgaagagctg cagagccgcg cccaggaagc gctggaccgc cgcgaagccg cctaccgcga
     1021 acagcgcgtg gcgctgggcg tggaagacgc cctggccgaa atgccgcacc tgaccgagca
     1081 gatgctggtc accctgggca aggccgggat caagacgctg gacgacctgg cggacctcgc
     1141 caccgacgag ctgatcgcca agaagcgcac cgaacagcgc cgccgcgaag gcagcgctcc
     1201 gccgcgccgc agcgaacgcg ccgaagacaa gggcggcgtg ctgggcgaat acggcctgtc
     1261 ggaagagcag ggcaacgaaa tcatcatggc cgcgcgcgcg cactggttcg aagacgagcc
     1321 agccacagag gaggccgcaa atgcggattc ctcacaatga gccgctaggt tccggcacct
     1381 cgaagcagcc ggcctcgggc gatcccgagc gccgctgcat cctgaccggg gaccacgggt
     1441 cccgggatga tctgctgcgc ctggcgatct cgcccgatgg cctggtcctg cccgatgctc
     1501 tggcgcgtgc gccggggcgg ggggcctgga tcggggtgtc gcgcgccgat ctggaaacgg
     1561 cgctggccaa gggcaagctc aagggcgccc tggcccgggc cttcaagggc gcggagctgt
     1621 ccattcccga tgacctgccg cagcgggccg aggccgcctt gcggcgcgcc ttcaccgatc
     1681 ggctggggct ggaaatgaaa agcggcaagt tgctgctggg ctctgaccgg atcgccgaga
     1741 acgcgcggat gggcaatgtc gcctggctgg cccatgccgc cgatgccagc gacgatggct
     1801 cgcgcaagct ggaccaggcc tggcgcgtgg gccgcgaaac cgaaggctcc ggggaaaccg
     1861 gattaacctt gcctctggac cgggcggcgc tctctgtggc attgggccgc gagaatgtcg
     1921 tccacctggc gctgaccgac ccccggtccg cggaaagggt agatgctccg ctccggcgcc
     1981 tgctgcgctt tctggggcag ggccacacga acgaacaggg caggggcgat tccgcggccg
     2041 atccggccgg gccatcgcct gtgacgacga attgagattt gaagggctga aagacgtatg
     2101 agcgatagcg ataacaaacc gactctgggc cgcaagccgc tgggcctgaa gaagtcggtc
     2161 gaagcgggcc aggtccagca gaccttcagc cacggccgca ccaacaaggt ggtggtcgag
     2221 gtgaagcagc gcaaactcat ccgcaagccg ggtgaggcgc cggtcgccgc tccgccgccg
     2281 ccgccgccgg ctccggcccc cgctccggtt gccgcagcgc ccaagcccgc gcccaagccg
     2341 gccgcccctg ccggggaaac cccgcaggaa cgcgtcgccc gcctgcagcg cgaggccgag
     2401 gaagcgcgcc tggcgatgct ggaagagcag acccgccgcg aacaggaaga gcgtctgcgc
     2461 gcccaggaag aggaaaagcg ccgggccgag cagaaccgca aggccgaagc cgatgccgcc
     2521 gctgccgcct ccgcgcccgc ggctgagccc gcggccgaac cggacgccgc tccggatgcc
     2581 gaacccgaag ccgaagcgcc gcgcggcgaa gcccccgcgc cgcgccgctt taccccggtg
     2641 gccgcgccca agcgccccga ggccccggcc aagaagatcg tccacccgcg cgacaagacc
     2701 ggcggcgatc agcgccgcca cgccggcaag ctgaccgtca gccgcgcgct gaacgaggac
     2761 gagggcgccc gcgcccgcag cctcgccgcg ctgaagcgcg cccgcgaaaa ggaacgccgc
     2821 gcccacttct ctggccagag ccagccgcgt gagaagcagg tccgtgacgt ggtcgtgccc
     2881 gaagcgatca ccgtgcagga actggccaac cgcatggccg aaaagggcgc ggatctggtc
     2941 aagtcgctgt tcaagatggg catgatggtc accgtcaacc agaccatcga tgccgacacc
     3001 gccgagctgc tggtcgagga attcggccac aacgtgcagc gcgtctcgga aagcgatgtc
     3061 gatatcgaca ccgccgagga cgtcgatgcg cccgaaacgc tgaaggcccg gccgccggtc
     3121 gtcaccatca tgggccacgt cgaccacggc aagaccagcc tgctcgacgc gctgcgcggc
     3181 accgatgtgg tgcgcggcga agccggcggg atcacccagc atatcggtgc ctaccagatc
     3241 aagaccaagg gcggcgatct ggtgaccttc ctggataccc cgggccacga agccttcacc
     3301 gaaatgcgcc agcgcggcgc caacgtgacc gacatcgtga tcctggtggt ggccggcgac
     3361 gacgggctga tgccgcagac gatcgaagcg atcaaccaca ccaaggcggc cggcgtgccg
     3421 atgatcgtgg cgatcaccaa gagcgacaag ccggaattca acccgcagaa gatccgcgag
     3481 cgcctgctgg agcacgaagt gatcgtcgag gcgatgagcg gtgacgtcca ggacgtcgaa
     3541 gtttcggcca agaccggcgc gggcctcgac aacctgatcg agaagatcct gctccagtcg
     3601 gagcttatgg agctgaaggc caacccggac cgccccgccg aagcgaccgt gatcgaagcc
     3661 aagctcgaca agggcaaggg gccgctggcc actgtgctga tcacccgcgg cacgctcaag
     3721 accggggata tcctggtggt tggcacccat tcgggccgag tgcgtgccat gcttgatgac
     3781 aagggccgcc agatcaaggt cgctccgccg gcgctgccgg tcgaagtgct gggcatcagc
     3841 ggcgtgccga tggctggcga taccctgacc gtggtggaaa gcgaggcacg cgcccgcgaa
     3901 gtggcc
//
LOCUS       DASUDV010000046         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_45620,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000046 DASUDV010000000
VERSION     DASUDV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45620"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFV30_00965"
     CDS             <1..1010
                     /locus_tag="VFV30_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4846687.1"
                     /translation="FREADVARARDQQLAGIAQANASPSALANRSLGPILFGPAHPYG
                     QPSDGLGDARSVGSLTPAALRTAQAEWLRPDNLTITVVGDVSMEALLPMLEASFGTWQ
                     APATPRGTKSLDAAIPAPRPRIVLIDRPGSPQSVIVAGRVLPLTGRDQGLEPLELANE
                     VLGNNFLSRLNLDLREEKGWSYGVRSVVRSPRGPRSLVVIAPVQSDKTGPAITAIRGQ
                     MAGFPAKKGVDGTEFNRATDGNIRGLPIRYETNGQVLGALVSNQALGRPDNYQATLPS
                     RYRAIDRQAIDAAAKQYLQPDGLVFVVVGDRKTVEPQLKDLGLPVEIATPVDSAPANG
                     E"
     gene            1063..1362
                     /locus_tag="VFV30_00970"
     CDS             1063..1362
                     /locus_tag="VFV30_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846688.1"
                     /translation="MSVAGTYECVTKTPMGDQKSDVTIVVDGDTFTGTNAGAMGSMEM
                     ENGKVDGNKLTWTMKMTVPMPMTLEGEATIDGDTLTGGVKAGAFGTFPITGTRKA"
     gene            complement(1441..>2077)
                     /gene="gatB"
                     /locus_tag="VFV30_00975"
     CDS             complement(1441..>2077)
                     /gene="gatB"
                     /locus_tag="VFV30_00975"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238999.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX4846689.1"
                     /translation="FPDPDLLPLELDDAFIEECKASLPELPDAKRHRYETALGLSAYN
                     AAALTADVEVARQFEALLTSVAKAGGKGEADLSKRVANWLLSEAPGVLNAAGVTSDHP
                     KNTVEANTAIVVATESGLISGSKAKEIFEAYLTGDVDLAAEIEANKQTSDTGAIEAAV
                     DAVLAANADKVAEYKGGKEALFGFFVGQTMKAMQGKGNPQLVNQVLKTKLG"
BASE COUNT          336 a          715 c          693 g          333 t
ORIGIN      
        1 ccttccgcga ggccgatgtg gcccgcgccc gggatcagca gctggccggg attgcccagg
       61 ccaatgccag cccctcggcg ctggccaacc gctcgctcgg gccgatcctt ttcggcccgg
      121 cccaccctta cggccagccc agcgacgggc tgggcgatgc ccgctcggtc ggcagcctca
      181 ccccggcggc gctgcgcacg gcccaggccg aatggctgcg gcctgacaac ctgaccatca
      241 ccgtggtcgg cgacgtttcg atggaggcgc tgctgccgat gctggaggcc tcgttcggca
      301 cctggcaggc cccggcgacg ccgcgcggga ccaagtcgct tgacgccgcg atccccgcgc
      361 cgcgcccgcg gatcgtcctg atcgaccggc ccggttcgcc ccagtcggtg atcgtcgccg
      421 ggcgggtcct gccgctgacc gggcgcgacc aggggctgga gccgctggaa ctggccaacg
      481 aagtgctggg caacaacttc ctcagccgcc tcaaccttga tctgcgcgag gagaagggct
      541 ggtcctatgg cgtgcgcagc gtggtgcgca gccccagggg gccgcgcagc ctggtggtga
      601 tcgccccggt ccagtccgac aagaccggcc ccgcaatcac cgcgatccgc ggccagatgg
      661 cgggcttccc ggccaagaag ggcgtggacg ggaccgagtt caaccgcgcc accgatggca
      721 acatccgcgg cctgccgatc cggtacgaaa ccaacggcca ggtgctgggc gcgctggtgt
      781 ccaaccaggc gctgggccgg ccggacaact atcaggccac cctgcccagc cgctaccgcg
      841 cgatcgaccg ccaggcgatc gacgccgcgg ccaagcagta cctccagccc gatggcctgg
      901 tcttcgtggt ggtgggcgat cgcaagacgg tggagcccca gctgaaggac ctgggcctgc
      961 cggtggagat cgcaaccccc gttgactctg cgcccgcaaa cggcgagtga ttgcgctact
     1021 tacccccctg taagttcgac acacagtttt aggagagaac acatgtctgt tgccggcacc
     1081 tacgaatgcg tgaccaagac cccgatgggc gaccagaaga gcgacgtgac catcgtcgtt
     1141 gatggcgaca ccttcaccgg caccaacgcc ggcgcgatgg gttcgatgga aatggaaaac
     1201 ggcaaggtcg atggcaacaa gctgacctgg acgatgaaga tgaccgtccc gatgccgatg
     1261 accctggaag gcgaagccac catcgatggc gacaccctga ccggcggcgt caaggctggc
     1321 gcgttcggca ccttcccgat caccggcacg cgcaaggcct gagccctcgc gcaccgcgaa
     1381 acacgaaggg cgccggaggg aaacctccgg cgcccttttc gtttgcccag tcggagcggg
     1441 tcagcccagc ttggtcttca gcacctggtt gaccagctgc gggttgccct tgccctgcat
     1501 ggccttcatc gtctggccga cgaagaagcc gaacagggct tccttgccgc ccttgtattc
     1561 ggccaccttg tcggcgttcg cggccagcac ggcgtcgacc gcggcttcga tcgcgccggt
     1621 gtcgctggtc tgcttgttgg cctcgatctc ggcggccaga tcgacatcgc cggtcaggta
     1681 ggcttcgaag atttccttgg ccttggagcc ggaaatcagc ccgctttcgg tggcgacgac
     1741 aatcgcggtg ttggcctcga ccgtgttctt cgggtggtcg ctggtcaccc cggcggcgtt
     1801 gagcacgccg ggcgcttcgg acagcagcca gttggcgacc cgcttcgaca ggtcggcctc
     1861 gcccttgccg ccagccttgg cgaccgaggt cagcagcgct tcgaactggc gcgccacttc
     1921 gacatcggcg gtcagcgcgg cggcgttgta ggccgaaaga cccagcgcgg tttcatagcg
     1981 gtggcgcttg gcatcgggca gttcgggcaa gctggccttg cattcttcga tgaacgcatc
     2041 gtcgagttcc agcggcagca ggtcaggatc ggggaag
//
LOCUS       DASUDV010000047         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_443337,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000047 DASUDV010000000
VERSION     DASUDV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443337"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..743)
                     /locus_tag="VFV30_00980"
     CDS             complement(282..743)
                     /locus_tag="VFV30_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846690.1"
                     /translation="MTSAARLVLCSAALLTLGAAPSPGKGTPYSLVIGDLSRQKDAPG
                     SPCEPDEDLCMDVMLETRLWNVEVLAGNETNSRLRVWHRAHTPYRWGARMQLAMIVGP
                     SSKGIRRGINLGTPTNGRVCVDESWFLPNPEGMRIPRGHSVNEDGDVCFTV"
     gene            complement(826..>2083)
                     /locus_tag="VFV30_00985"
     CDS             complement(826..>2083)
                     /locus_tag="VFV30_00985"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX4846691.1"
                     /translation="DEGKGKIVDWLASRADAVVRFQGGHNAGHTLVVGENVYKLSLLP
                     SGIVTGTLSVIGNGVVLDPWHFRDEVAKLKGQGVDVNPQNLQVAENAPLILPFHRDLD
                     ALREDASGAGKIGTTRRGIGPAYEDKVGRRSIRVCDLAHLDDLGPQLDRICAHHDALR
                     AGFGQPPIDRQRLLDDLWDIAAFVLPFAKPVWRTLGECKKAGKRILFEGAQGVLLDVD
                     HGTYPFVTSSNTVSGTAASGSGLGPSAAGFVLGIVKAYTTRVGSGPFPTELEDETGQR
                     LGERGHEFGTVTGRKRRCGWFDAVLTRQSCAISGVTGIALTKLDVLDDFETVKICVGY
                     KLRGADMDYFPAHAADQAAVEPVYEEMEGWKGQTTAGARSWADLPAQAIKYIQRVQEL
                     IETPVALVSTSPEREDTILVRDPFVD"
BASE COUNT          345 a          715 c          675 g          348 t
ORIGIN      
        1 atcgactccg tagagcgtca tccgccgcgc ggccttttcg accaggatcc ggtcgacttc
       61 gggataggag accggcgggg cgggcgccgc cgaaccagac agcagcaaag cggcgatggc
      121 agttgttgcg cgaaacagca tcggcccgtt cctgagacag gattgtgcgg acaaatgtgc
      181 cagaacgcgg cccgggcgca aagtcggcat tctgtcagcg ggttgtgccg atccagtgca
      241 ggcccgtagg cctcaatccg ccacgccatt ccgcgttcgg atcagaccgt gaagcagacg
      301 tccccgtctt cgttgacgct gtgcccgcgc gggatccgca tgccttccgg gtttggaagg
      361 aaccaggact cgtcgacaca gacccggccg ttggtcggcg tgcccaggtt gatgccacgg
      421 cgtatgcctt tggacgacgg tccgacgatc atcgccagct gcatgcgggc gccccaccgg
      481 tagggtgtat gcgcgcggtg ccaaaccctc agccgcgagt tggtctcgtt gccggccaac
      541 acttccacgt tccagagccg ggtttccagc atgacatcca tgcacaggtc ttcgtccggc
      601 tcgcagggac tgccgggcgc gtccttctgc cgggaaaggt caccaatcac cagcgagtaa
      661 ggcgtccctt tgccgggcga aggcgcggcc cccagcgtca gcaaggccgc tgagcacaga
      721 accagccgcg cagcgcttgt catgatccgg ccccttccca tcggtgtgat caccgcaaag
      781 gggtatggcg aaccgggccg gtgcgcaagg gtactgtccg acccctcaat ccacgaacgg
      841 atcgcgcacc aggatcgtgt cctcgcgttc cgggctggtc gaaaccagcg cgaccggcgt
      901 ttcgatcagt tcctggaccc gctggatgta cttgatcgcc tgggccggca gatcggccca
      961 gctgcgcgcg ccggcggtgg tctggccctt ccagccttcc atttcctcgt agaccggctc
     1021 aaccgcggcc tgatcggcgg cgtgagccgg gaaataatcc atgtcggccc cgcgcagctt
     1081 gtaacccacg cagatcttca ccgtctcgaa atcgtcaagc acgtcgagct tggtcagcgc
     1141 aatcccggtc acgccgctga tcgcgcagct ttgccgggtc agcaccgcat cgaaccagcc
     1201 gcagcggcgc ttgcggccgg tgacggtgcc gaattcgtgg ccgcgctcgc ccaagcgctg
     1261 gccggtctcg tcctccagct cggtcgggaa cgggcccgat ccgacgcggg tggtataggc
     1321 cttgacgatc cccagcacga aaccggcggc gctggggccc aggcccgaac cgctggccgc
     1381 ggtgccgctg accgtgttgg agctggtgac gaagggatag gtgccgtgat cgacatcgag
     1441 cagcacgccc tgcgcgcctt cgaacaggat ccgcttgccg gccttcttgc attcccccag
     1501 cgtgcgccac accggcttgg caaagggcag gacgaaagcg gcgatatccc acaggtcatc
     1561 cagcagccgc tggcggtcga tcggcggctg gccgaacccg gcgcgcagcg catcgtggtg
     1621 ggcgcagatc cggtccagct gcgggccgag atcgtccagg tgcgccagat cgcaaacccg
     1681 gatcgagcgg cggccgacct tgtcctcata ggccgggcca atcccgcggc gggtggtgcc
     1741 gatcttgcct gccccgctgg cgtcttcgcg cagggcatcc agatcgcggt ggaacggcag
     1801 gatcagcggg gcgttttcag cgacctgcag gttctggggg ttcacatcaa ccccctgccc
     1861 cttgagcttc gccacctcat cccggaagtg ccagggatcc agcaccacgc cgttgccgat
     1921 caccgaaagc gtgccggtga caatgcccga gggcagcagg ctgagcttgt agacgttctc
     1981 gcccaccacc agcgtgtggc cggcgttgtg gccgccctgg aaccgcacca cggcatcggc
     2041 ccggctggcc agccagtcga cgatcttgcc cttgccctca tcg
//
LOCUS       DASUDV010000048         3696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_863111,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000048 DASUDV010000000
VERSION     DASUDV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863111"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFV30_00990"
     CDS             complement(<1..573)
                     /locus_tag="VFV30_00990"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413079.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX4846692.1"
                     /translation="MSFTADRLLLFGATGDLAQRMLLPSLCALHADKLVAEDLEIVGT
                     ARQPLDDAGFRNFAREALEKFMPADRRGGMATFLNRLTYQPLDATDMAGYAALAAKVG
                     QPKQGLAIFLSTAPSLFEPTIAGLTAGGLAGETVRIGLEKPLGTSLESSREINDAVAR
                     AFPEDRTFRIDHYLGKETVQNLLALRFANIL"
     gene            766..2187
                     /locus_tag="VFV30_00995"
     CDS             766..2187
                     /locus_tag="VFV30_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4846693.1"
                     /translation="MRRTKIAVGIALACALAVPQLAARPALLKMAPMPQAEAQSLDLA
                     KQLIALRSVRGPGNETGKALGVVKSALVQGGWQDSDVEIVPVDDTAYMIATWPGSDPK
                     LKPLVISGHMDVVEADPKDWTRDPFTPIIEDGILYGRGASDMKFDAAVATSSLIELRR
                     QGYKPKRTIILQFSGDEETTMKTSKIIAQRLKHAELVINIDGGGGTLDEATGKPLFWT
                     WQGAEKTYVDYQLEVTNPGGHSSAPRPVNAISQMSQALDRIGAYHFKPELSPLTKAYF
                     EKAAQFETDPKLAAAMRAFAANPADEAALAVLRANPAYVGKIGTTCVATMVHGGHAQN
                     ALPQRVTANVNCRIFPGHTREEIRSELARVVGSDAVKITDVTGEDSTESPASPMRPDF
                     IAAVERAIGRTYPGVAIIPSQSSGATDSMWYRALGVPSYAASPTFSKDSEDFAHGLNE
                     RVRLSNIRPGLVYYLSLFTDLSK"
     gene            complement(2168..2773)
                     /locus_tag="VFV30_01000"
     CDS             complement(2168..2773)
                     /locus_tag="VFV30_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX4846694.1"
                     /translation="MAPQPAFRRAEPDERRASLVEACARVLAREGAAGASVRAIAVEA
                     GVSPGLIGHYFGGVDALVAATYAHVEAQVAAALDRAVDAAGPDPRARLDAYVSASLLP
                     PIADSELLSTWIAFWSLVRARPEIAALHDEQYGRFRAGLEALLTANGTPPGDLRRTAI
                     AVTALVDGLWLELCLSPGTFAPGEAAAIAVEGLAALISTGR"
     gene            2827..>3696
                     /locus_tag="VFV30_01005"
     CDS             2827..>3696
                     /locus_tag="VFV30_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4846695.1"
                     /translation="MTKLDHAFAAIAGDDVDPDADFSLPGWIYHDREFFDLELERIFR
                     PSWQIVCHVSEVAGPGDYRTLDYLGESVIVIRGDDGQIRAFTNVCRHRAMRLVEGPSG
                     CTKKLICPYHAWTFETDGRLTGVPMKSEYPALKLEENGLVPVALEIWHGFVFVCLQPE
                     GFPTPGEMMAPFEDEVAQYRFEDMLPMGDVRHRPRAVNWKNVGDNYSDNLHIPVAHDG
                     LTRLVGKTYRIEAHAWADRLTGDVVKNEKQNFWETFYRAHMPRVDHLPEEAHGRWLYY
                     KLWPNMAFDIYADQ"
BASE COUNT          632 a         1240 c         1255 g          569 t
ORIGIN      
        1 caggatgttg gcaaagcgca gcgcgaggag gttctggacc gtttccttgc ccagatagtg
       61 gtcgatccgg aaggtccggt cctcggggaa ggcgcgggcc acggcgtcgt tgatctcgcg
      121 gctcgattcc aggctggtgc caagcggctt ttccagcccg atccgcaccg tttcgccggc
      181 caggccgccg gcggtcagcc cggcaatggt cggctcgaac aggctcggcg cggtcgacag
      241 gaagattgcc aggccctgct tgggctggcc gaccttggcc gccagcgcgg catagccggc
      301 catgtcggtg gcatcgagcg gctgataggt taggcggttg aggaaggtcg ccatgccgcc
      361 gcgccggtcg gccggcatga atttctccag cgcctcgcgc gcgaagttgc gaaagcccgc
      421 gtcgtcgagc ggctggcgtg cggtgccgac gatttccagg tcctccgcca ccagcttgtc
      481 ggcgtgaagg gcgcacagcg aaggcagcag catccgctgg gcaaggtcgc cggtggcgcc
      541 gaacagcagc aggcggtcag cagtaaagct catggccgat cctctcgccc cgccggaact
      601 ggcgcgggac ccggccgggt tagcagcgca gcggcgggct tgccagtgcg gcagggggcc
      661 ttgggaggca tcgcaaaggc tgaatacgtc tgcgatttgg cctgacgcgg cggcttccca
      721 cgatccgggt caggctctaa gcctgcgggc aaaggagatt cgccgatgcg ccgtaccaag
      781 atcgccgtgg ggattgccct ggcttgtgcc ctcgccgtgc cccagctggc ggcccgtccg
      841 gccctgctga agatggcacc gatgccccag gccgaggcgc agtcgctgga cctggccaag
      901 cagctgatcg cgctgcgctc ggtccgcggc cccggcaacg aaaccggcaa ggcgctgggc
      961 gtggtcaagt ccgcgctggt ccagggcggc tggcaggaca gcgatgtgga gatcgtgccg
     1021 gttgacgaca ccgcctatat gatagccacc tggccgggca gcgatccgaa gctcaagccg
     1081 ctggtcattt ccggccacat ggacgtggtc gaggccgatc ccaaggactg gacccgcgat
     1141 cccttcaccc cgatcatcga ggacgggatc ctctatggcc gcggggcgag cgacatgaag
     1201 ttcgacgcgg ccgtcgccac ctccagcctg atcgagctgc gccgccaggg ctacaagccc
     1261 aagcgcacga tcatcctgca attctcgggc gacgaggaaa cgacgatgaa gacgtcgaag
     1321 atcatcgccc agcggctgaa gcacgccgaa ctggtgatca acatcgatgg cggcggcggg
     1381 accctcgacg aggcgaccgg caagccgctg ttctggacct ggcagggcgc ggaaaagacc
     1441 tatgtcgatt accagctgga agtgaccaac ccgggcgggc acagctcggc gccgcgcccg
     1501 gtcaacgcga tcagccagat gtcgcaggcg ctggaccgga tcggggccta tcacttcaag
     1561 ccggaactga gcccgctgac caaggcctat ttcgaaaagg ccgcgcagtt tgaaaccgat
     1621 cccaagttgg ccgcggcgat gcgcgccttt gcggccaatc cggcggacga agccgcgctg
     1681 gccgtgctgc gcgccaatcc ggcctatgtc ggcaagatcg gcaccacttg cgtcgcgacc
     1741 atggtccacg gcgggcacgc ccagaacgcg ctgccgcagc gggttaccgc caacgtcaat
     1801 tgccggatct tccccggtca cacccgcgag gagatccgca gcgagctggc ccgcgtggtc
     1861 ggcagcgacg cggtcaagat caccgacgtg accggcgagg attcgaccga aagtccggcc
     1921 tcgccgatgc ggcccgattt catcgcggcg gtggaacgcg cgatcgggcg gacctatccg
     1981 ggcgtggcga tcatccccag ccaatcctcg ggcgcgaccg attcgatgtg gtaccgggcg
     2041 ctgggcgtgc ccagctatgc cgccagcccg accttcagca aggattcgga agacttcgcc
     2101 cacggcctga acgagcgggt gcggctgtcg aacatccgcc ccggtctggt ctattacctg
     2161 agcctgttca ccgacctgtc gaaataaggg cggccaggcc ctccacggca atcgcggccg
     2221 cttcgcccgg ggcaaaggtc ccgggcgaaa ggcacagctc cagccacagc ccgtcgacca
     2281 gcgcggtcac cgcaatcgcg gtgcggcgca ggtcgcccgg cggggtcccg tttgcggtca
     2341 gcagcgcttc cagcccggcg cggaagcggc cgtactgctc gtcgtgcagc gcggcgattt
     2401 cggggcgggc gcggaccagg ctccagaagg cgatccaggt ggacagcagc tcgctgtcgg
     2461 cgatcggcgg gagcaagctg gcgctgacat aggcgtccag ccgcgcgcgc ggatcgggtc
     2521 cggcggcgtc caccgcgcgg tccagcgcgg cggccacctg cgcttcgaca tgggcatagg
     2581 ttgccgcgac cagcgcatcg acgccgccga aatagtgccc gatcagcccc ggcgagaccc
     2641 cggcctccac cgcgatcgcc cgcacgctgg ccccggccgc gccttcgcgg gcgaggacgc
     2701 gggcacaggc ctcgaccagg ctggcgcggc gctcgtcggg ctcggctctg cggaatgcgg
     2761 gttgcggtgc catcgcgctt gttatacgat cgttcaacaa ttcgatcaag gattcgctgg
     2821 ggacctatga ccaagctcga tcacgccttt gccgcgatcg cgggggacga tgtcgatccc
     2881 gatgccgatt tcagcctgcc gggctggatc tatcacgacc gcgaattctt cgatctggag
     2941 ctggagcgga tcttccgccc ttcctggcag atcgtctgcc acgtcagcga agtggccggc
     3001 cccggcgatt accgcacgct cgactatctg ggcgagagtg tcatcgtcat tcgcggcgat
     3061 gacggccaga tccgcgcctt caccaacgtc tgccgccacc gcgcgatgcg tctggtagag
     3121 ggtccttcgg gttgcacgaa gaagctgatc tgcccctatc acgcctggac cttcgaaacc
     3181 gacgggcggc tgaccggggt gccgatgaag tccgaatatc cggcgctcaa gctggaagag
     3241 aacggcctcg tccccgtcgc gctggagatc tggcacggct tcgtcttcgt ctgcctgcag
     3301 ccggagggtt tcccgacgcc cggcgaaatg atggcgccgt tcgaagacga ggtcgcgcag
     3361 taccggttcg aggacatgct gccgatgggc gacgtccgcc accgcccgcg cgccgtcaac
     3421 tggaagaacg tgggcgacaa ctattcggac aacctgcaca tcccggtggc gcacgacggg
     3481 ctgacccggc tggtcggcaa gacctaccgg atcgaggcgc acgcctgggc cgaccggctg
     3541 accggcgacg tggtgaagaa cgagaagcag aacttctggg agaccttcta ccgcgcccac
     3601 atgccgcggg tcgatcacct gcccgaagag gcccacggcc gctggctcta ttacaagctg
     3661 tggccgaaca tggccttcga tatctacgcc gaccag
//
LOCUS       DASUDV010000049         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2011890,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000049 DASUDV010000000
VERSION     DASUDV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011890"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFV30_01010"
     CDS             <1..566
                     /locus_tag="VFV30_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX4846696.1"
                     /translation="PDGRHRSAVVWTFDEKDAKGVIALPDAVFLGEMAQRMEGIMGRI
                     ELIAPRMSYPLNFHHAPRIVAQRLALVGDAAHGMHPIAGQGLNLGLRDVGVLVEVLSE
                     GMRLGLDPGDAQVLARYERWRSVDSVTVMATTDALVRLFGIPGRLPSAARRLGMGVVQ
                     RSGWLKRLFMDEARGMSGDLPALLKAA"
     gene            complement(573..1484)
                     /locus_tag="VFV30_01015"
     CDS             complement(573..1484)
                     /locus_tag="VFV30_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846697.1"
                     /translation="MTYDSYSSTQGARRGGLSLRGALILILLAFAAGAALLGWLVRDG
                     RITLSASDAAPEPTPVASSGFAKLPAPSPSVAASQAAATAGFETRVAALEARLARLDL
                     EAAAAEGNAARAEALMVAFAARRVVERGAELGFLAEQVKLRFGDAQPGAVATVLEAAK
                     QPVTLDRLAGDLDNLGPVLAQTAKDEDGWSRFKRELSGLFVIRRDDTPSARPQDRFER
                     ARLLLRTGQIDAAVTEVERLPGAAAAGPWIGQARRYVEVQRALEQLETAALLEPERLR
                     GSGGEALRQPSPASAAPAPKDAASASF"
     gene            complement(1507..2196)
                     /locus_tag="VFV30_01020"
     CDS             complement(1507..2196)
                     /locus_tag="VFV30_01020"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX4846698.1"
                     /translation="MVPLVVIRPEPGCSNTVAAARAAGIACHGFPLFEVAARSWEGLA
                     PQGFDALLIGSANALRHGGPGLAALRALPVLAVGETTAAEARAAGFSVAQTGAGGLQS
                     LLDNLPTRCTRLLRLGGEERVPLNPPKGVSIEERVVYATRSLPFPPDLAALLASPAII
                     ALHSADAARHLAAQCVSHAIRRARLRLVALSPRIAQAAGEGWGEVAAAANPDDKALLA
                     LARQMCQDPWP"
     gene            complement(2200..>3007)
                     /gene="hemC"
                     /locus_tag="VFV30_01025"
     CDS             complement(2200..>3007)
                     /gene="hemC"
                     /locus_tag="VFV30_01025"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX4846699.1"
                     /translation="VTASGDRIQDRPLAEIGGKALWTKELDAWLLAGEIDLAVHSAKD
                     VETLRPADIAIAAVLPREDVRDVLIGAPSLAALPPGARIGTSAPRRAAQLLHLRPDCE
                     VVSFRGNVATRLAKLAGGEADATLLAKAGLNRLGQGETGTALDPAEWLPAPGQGAIAI
                     ECRAGDDATRALLAAIDHPTSHRALLAERALLAALGGNCHSPIAVLTSDEGDDLVMRA
                     ALFSPDGAHKIEQELRFAITDLDGPARLARQLLAEAPEAVRCHFSGQAGD"
BASE COUNT          469 a         1015 c         1066 g          457 t
ORIGIN      
        1 tgcctgacgg ccgccatcgc agcgcggtgg tctggacctt cgatgagaag gacgcgaagg
       61 gcgtgatcgc gctgcccgat gcggtgttcc tgggcgaaat ggcgcagcgc atggaaggga
      121 tcatggggcg gatcgaactg atcgcgccgc gcatgtccta tccgctcaat ttccaccatg
      181 ccccgcggat cgtcgcgcag cggctggcac tggtcggcga tgccgcacac ggcatgcacc
      241 cgatcgccgg gcaggggctt aaccttggcc tgcgcgatgt cggcgtgctg gtcgaggtgc
      301 tgagcgaagg gatgcggctg ggcctcgatc cgggcgatgc ccaggtcctg gcccgctacg
      361 aacgctggcg cagtgtcgat agcgtaaccg tgatggccac caccgatgcg ttggtccgtc
      421 tattcggcat tcccggacgc ctgcccagcg cggcgcggcg gctgggcatg ggtgtggtgc
      481 agcgttcggg ctggctcaag cggctgttca tggacgaggc acgcgggatg agcggcgatc
      541 tgcccgcgct gctgaaggcg gcctagaaac ccttagaagg aggcgctggc cgcgtccttg
      601 ggagctggcg cggcggaagc cgggctgggc tggcgcaagg cctcaccgcc tgatccgcgc
      661 agccgttccg gttcgagcag ggctgcggtt tcgagctgtt cgagcgcgcg ctgcacttcg
      721 acatagcggc gtgcctgacc gatccatgga cccgccgcag cagcaccggg caaacgctcg
      781 acctcggtta ccgccgcatc aatctggccg gtgcgcagca gcagccgggc ccgttcgaag
      841 cggtcctgcg ggcgggccga tggcgtatca tcgcggcgga tcacgaacag gccggaaagc
      901 tcgcgcttga accggctcca gccgtcctcg tccttcgcgg tctgggccag aaccgggccc
      961 agattgtcga gatcgccggc caggcgatcc agcgtaaccg gctgctttgc cgcttccagc
     1021 accgtggcca cggcaccagg ctgggcatcg ccgaaccgca gtttgacctg ttcggcaagg
     1081 aatcccagtt cggcgccgcg ttcgaccacc cggcgcgcgg caaaggccac catcagcgct
     1141 tcggcgcgcg cggcattgcc ttcggcggca gcggcttcaa gatcgaggcg ggcaaggcga
     1201 gcttcaagcg cggcaacgcg ggtttcgaaa ccggcggtgg cggcggcctg gctggctgca
     1261 accgatggac taggcgcggg cagcttggca aagccggaac tggcgaccgg cgtgggttcg
     1321 ggtgcggcat cgctggcgga aagcgtgatc cgcccgtccc gcaccagcca gccaagcagt
     1381 gcggcgccgg ccgcgaatgc cagcaggatc aggatcagcg cgccgcgcaa ggaaagtcca
     1441 ccgcgccggg cgccctgggt acttgaatag ctgtcgtagg tcatgctctt gcagcgattt
     1501 cccggttcaa ggccaggggt cttggcacat ctggcgggcc aaggccagca atgccttgtc
     1561 atcggggttg gccgcggcgg caacttcgcc ccaaccttcg cccgcggcct gggcgatgcg
     1621 cggcgaaagc gcgaccagcc gcaaccgggc ccggcggatc gcgtggctca cgcattgagc
     1681 ggcaaggtgc cgggcggcgt cggcgctgtg cagcgcgatg atcgccgggc tggccagcag
     1741 cgcggcgagg tctggcggga aaggcagcga acgcgtggca tagaccacgc gctcctcgat
     1801 gctcaccccc ttgggcgggt tcagcggcac ccgttcctcg ccgcccagcc gcagcaaccg
     1861 ggtgcagcgg gtgggcagat tgtcgagcag gctctgcaat ccgcccgcgc ccgtttgcgc
     1921 caccgaaaat ccggcggcgc gcgcctccgc cgcagtggtt tcgcccacgg ccagaactgg
     1981 cagggcgcgc aaggcggcca atcccggccc gccatggcgc agggcgttgg cgctgccgat
     2041 cagcagggcg tcaaagcctt ggggagcaag gccttcccag cttctggccg caacctcgaa
     2101 cagtggaaag ccgtggcatg cgatccccgc cgcgcgcgcc gccgcaaccg tgttgctgca
     2161 gccgggttcg ggacggatca cgaccagcgg caccatcgat cagtcccccg cctgtcctga
     2221 gaaatggcag cgaaccgcct cgggtgcctc ggccaggagc tgccgggcga gccgcgccgg
     2281 tccatcaagg tccgttatag caaagcgcaa ttcctgctcg atcttgtgtg cgccatcggg
     2341 gctgaacagc gccgcgcgca tcacaagatc gtcgccctca tcgctggtca gcaccgcaat
     2401 cgggctgtga caattgcccc ccagtgcggc cagcagcgcg cgttcggcca gcagcgcgcg
     2461 gtggctggtg ggatggtcga tcgcggcaag gagggcgcgg gttgcatcat caccggcgcg
     2521 gcattcgatc gcgattgcgc cttgcccggg ggcgggcagc cattcggcag gatcgagcgc
     2581 ggttccggtc tcaccctggc ccagccggtt cagtccggcc ttggccagca aggtggcatc
     2641 ggcctcgccg ccagccagct tggccagccg cgtcgccaca ttgccgcgga aactcacaac
     2701 ctcgcaatcg gggcgcaggt gcagaagctg tgcggcgcgg cgcggcgcgc tggtgccgat
     2761 ccgcgcgccg ggcggcaggg ctgcaagcga aggtgcgccg atcagcacat cgcgcacatc
     2821 ctcacgcggc aagacggcgg caatcgcgat gtcggcaggg cgcagcgttt cgacatcctt
     2881 ggccgaatgg accgccagat cgatttcgcc cgccagcaac caggcgtcga gttccttggt
     2941 ccacagcgcc ttgccgccga tttcggccag cggacggtcc tggatcctgt cgccgctggc
     3001 ggttacc
//
LOCUS       DASUDV010000050         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1797,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000050 DASUDV010000000
VERSION     DASUDV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /gene="fliD"
                     /locus_tag="VFV30_01030"
     CDS             <1..643
                     /gene="fliD"
                     /locus_tag="VFV30_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX4846700.1"
                     /translation="SLTLTGTNPGAPATIRFSDPAAAASTFMNDLVGALNELAGQLKA
                     QTDPLSGDLARDSGARALRRALSQLAGSTVMPGASPGEPKTLADLGLATNRDGTFRLD
                     TARLTATLKASPDGVAAMFTTGINGVFATFDRLSRRLTAASDPGTLAGSLSRLTAQKR
                     DLGDEAAKLAEAQDALRARLSRQFAGVDSRVAASKSTLSFLQNQIDAWNAQKG"
     gene            645..1046
                     /locus_tag="VFV30_01035"
     CDS             645..1046
                     /locus_tag="VFV30_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HEX4846701.1"
                     /translation="MQILRSPQDVYRKVDLDARIAGADPQQLVLLCYEQLGTALGSAV
                     HAAQAGDNARKSAALTRALSAITALQMGLDPAAPVSAALGTFLDAARQAVLGSVIAFD
                     AGALERLRGDVGELAAAFRKAAGTRAMVRNG"
     gene            1193..1804
                     /locus_tag="VFV30_01040"
     CDS             1193..1804
                     /locus_tag="VFV30_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4846702.1"
                     /translation="MQKRYSLALVDGNSRRRAAITHALAGSGIHVEPFEDLGEITFRW
                     PRTGAILVADQGPVVDDLIGHMSLHGEWLPVIAFSEDPGTERIVRAVLAGALDYLEWP
                     CDPAKIVTAVTLAESASASIGSLRLREARARSRVQKLTRREREVLEGVADGLSNRLIG
                     EKLRISPRTVEIHRANMLNKMGASHTSEAIRIAIEASLVGQAA"
     gene            complement(1973..>2430)
                     /locus_tag="VFV30_01045"
     CDS             complement(1973..>2430)
                     /locus_tag="VFV30_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4846703.1"
                     /translation="EIARMGLMVSAQPNYIRVLGDAYGRQGLGPGRAEQINRLGSLER
                     KGVPLGLHSDFNMAPIDPFYLAWIAANRVTIEGQVKAPAERLSLDKALRAITIEAAQV
                     IGMDALVGSLSPGKRADLAVLDRDPYAGGAAGLRDVRVESVVFEGRPAG"
BASE COUNT          382 a          896 c          807 g          345 t
ORIGIN      
        1 tagcctgacc ctgaccggca ccaatcccgg cgcgccggcc acgatccgct tcagcgatcc
       61 tgcggcggcg gccagcacct tcatgaacga cctggtcggc gcgctcaacg agctggccgg
      121 ccagctcaag gcccagaccg atccgctgag cggcgacctg gcacgcgaca gcggtgcgcg
      181 ggcgctgcgc cgggcgctca gccagctggc cggcagcacg gtgatgcccg gcgcttcccc
      241 gggcgagccc aagaccctgg ccgaccttgg ccttgccacc aaccgcgacg gcaccttccg
      301 gctcgatacc gcgcggctga ccgccacgct gaaagcctcg cccgatggcg tcgcggcgat
      361 gttcaccacc gggatcaacg gcgtcttcgc caccttcgac cggctcagcc gccgcctgac
      421 cgccgccagc gatcccggca cgctggccgg atcgctgtcc cggctgaccg cgcaaaagcg
      481 cgatctgggc gacgaggcgg ccaagctggc cgaggcgcag gatgcgctgc gggcgcggct
      541 gtcacggcag ttcgccgggg tcgacagccg ggtcgccgcg tcgaaatcga cgctgtcgtt
      601 cctgcagaac cagatcgacg cgtggaacgc gcagaagggc tgacatgcaa atcctccgct
      661 ccccccagga cgtctaccgc aaggtcgatc tcgacgcccg gatcgccggg gccgatccgc
      721 agcaactggt cctgctgtgc tacgaacagc tcggcaccgc gctcggctcg gcggttcatg
      781 ccgcccaggc cggcgacaac gcccgcaaga gcgccgcgct gacccgcgcg ctctccgcga
      841 tcaccgcgct gcagatgggg ctggatcccg ccgcgccggt ctccgccgcg cttggcacct
      901 tcctcgatgc ggcgcgccag gcggtgctgg gcagcgtgat agcgttcgat gccggcgcgc
      961 tggagcggct gcgcggcgac gtgggcgagc tggccgcggc cttccggaag gccgccggga
     1021 cgcgggctat ggtccggaat ggttgaattg ccggcccgga tttttgcgcg cccggcgcgg
     1081 cagacctacg ggtttgtgcg gatttcatcg ctcgaatccg gagccacctt gatgcggccg
     1141 gcaccgcact tcggcctaat cccctaggcg ctaaccacta gggggcattc cgatgcagaa
     1201 gcgctacagt ctggctttgg tcgatggcaa cagccgccgc cgcgccgcga ttacccacgc
     1261 cctggcgggc agcggcatcc acgttgaacc gtttgaggat ctgggcgaaa tcaccttccg
     1321 ctggccgcgc accggggcga tcctggtcgc cgatcagggc ccggtggtgg acgacctgat
     1381 tggccacatg tcgctgcacg gtgaatggct gccggtcatc gccttcagcg aggatccggg
     1441 gaccgaacgg atcgtccgcg cggtgctggc cggcgcgctc gattatctgg aatggccctg
     1501 cgatccggcc aagatcgtca ccgcggtgac cctggccgaa agcgcctcgg ccagcatcgg
     1561 cagcctgcgc ctgcgcgaag cccgcgcccg cagccgggtg cagaagctga cccgccgcga
     1621 gcgcgaggtg ctggaagggg tggccgatgg tctgtccaac cggctgattg gcgaaaagct
     1681 ccggatcagc ccgcgcacgg tggagatcca ccgcgccaac atgctcaaca agatgggggc
     1741 cagccacacc agcgaagcga tccggatcgc gatcgaagcc tcgctggtcg gccaggccgc
     1801 ctaggcgttc tacctagccc ggggcaaacg ccgggataac cccggcatgg ctatcagggg
     1861 gttgcaccgg atggtgccaa gtccctggaa ggaagcagga aaccccgctc cacccctgcc
     1921 cccgctgccc cggccaaccg ttccccaatg cggccggggc agcaccccgc cttcacccgg
     1981 cagggcgccc ttcgaacacc acgctttcca cccgcacatc gcgcagcccg gccgcgccgc
     2041 cggcataggg atcgcggtcc agcacggcga ggtcggcccg cttgcccggc gacagcgagc
     2101 cgaccagcgc gtccatgccg atcacctgcg ccgcctcgat cgtgatcgcc cgcagcgcct
     2161 tgtcgaggct gagccgttcg gccggggcct tgacctgccc ttcgatggta acccggttgg
     2221 ccgcgatcca ggccagatag aacggatcga tcggcgccat gttgaaatcg ctgtgcagcc
     2281 ccagcggcac gcccttgcgc tccagcgagc cgagccggtt gatctgctcg gcccggcccg
     2341 ggccaagccc ctgccggcca taagcatcgc ccagcacccg gatgtagttg ggctgggccg
     2401 aaaccatcag gcccatgcga gcaatctccc
//
LOCUS       DASUDV010000051         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1746826,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000051 DASUDV010000000
VERSION     DASUDV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746826"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1809
                     /locus_tag="VFV30_01050"
     CDS             49..1809
                     /locus_tag="VFV30_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4846704.1"
                     /translation="MKSAFFRTVVAAALLGASMPALAAPADDLGQVIAEHWQWVLKES
                     PILASSVGDKRYDDKLPDISLAAADRAASQAGEFIKRLDAIPDAGLDGAARANKGILR
                     SLLKDQVDSNRFGQRTMLFSTYSSPHQYLAGLAENLAFDSGADYRNYLKRLEQIGPLN
                     QQTIAITREALAKGYVQPCLAMGGFENTVLGAVAGKAEQTRFFAPFNAARPRDLSESE
                     WSTLKSRAVEVIGTLIKPEFEKFAAFYVKDYKPRCRTDVGASSLPQGKEWYALQARQH
                     TTTSLTPEQIHQIGLDEVTRIRKRMDEVAAKAGFASRAAMIEALRTDPKYYARTPEEL
                     MAAASRQAKIIDGLMPRYFGLLPRLPYGVKEIPAETAETTTTAYYGPGSPQAGLAGNY
                     FVNTSKLPQRPLYELPALTVHEAVPGHHHQIALQQELNLPEFRRNIAFFTAFTEGWGL
                     YTEYLGEEMGIYDTPEKMMGRLSYEMWRACRLVVDTGMHAKGWTKAQAVQFMQDNTAL
                     SAANIEAEVNRYISWPGQALGYKIGEIKIRELRARAESELGAKFDLRRFHDAVLGQGS
                     VPLDVLEAQIADWIAAEKQR"
     gene            complement(1810..>2069)
                     /locus_tag="VFV30_01055"
     CDS             complement(1810..>2069)
                     /locus_tag="VFV30_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX4846705.1"
                     /translation="AIAARARTRRPAERADGKAVSPDERRALDQLGLSLDATRHDLRA
                     RYSALVRRYHPDRNGGDRSFEAKLGEVIAAYQLLRKAAIFA"
BASE COUNT          400 a          691 c          635 g          343 t
ORIGIN      
        1 ccggctccgt gccaaccacg cgcaccagcc gttcgattgg gggacaggat gaagtctgcg
       61 tttttccgca ccgttgttgc cgccgcgctg cttggcgcga gcatgccggc cctggccgcg
      121 cccgccgatg atctgggcca ggtaatcgcc gaacattggc aatgggtgct caaggaaagc
      181 ccgatcctcg cctcgagcgt gggcgacaag cgctatgacg acaagctgcc cgacatcagc
      241 cttgccgcgg ccgatcgcgc cgcctcgcag gcgggtgagt tcatcaagcg gctagatgcc
      301 attcccgatg ccgggcttga tggcgcggcg cgggccaaca agggcatcct gcgctcgctg
      361 ctgaaggatc aggtcgattc gaaccggttc ggccagcgca ccatgctgtt ttcgacctat
      421 tcgagcccgc atcagtatct ggctggcctc gccgaaaacc tggccttcga tagcggcgcg
      481 gattaccgca actatctcaa gcggctggaa cagattggcc cgctcaacca gcagaccatt
      541 gccatcaccc gcgaagccct ggccaagggc tatgtccagc cgtgcctggc gatgggcggg
      601 ttcgagaaca cggtgctggg cgcggtcgca ggcaaggccg agcagacccg ctttttcgcg
      661 cctttcaacg ccgcgcgtcc acgcgacctg agcgaaagcg aatggagcac gctcaagtcc
      721 cgcgcggtcg aggtaatcgg caccctgatc aaaccggaat tcgaaaagtt cgcggccttt
      781 tacgtcaagg attacaaacc caggtgccgc accgatgttg gtgcatcttc cctgccccag
      841 ggcaaggaat ggtatgccct gcaggcccgc cagcacacca ccaccagcct cacccccgaa
      901 cagatccacc agatcggcct ggatgaggtg acgcggatcc gcaagcggat ggacgaagtg
      961 gccgccaagg ccggcttcgc cagccgcgcc gccatgatcg aggcgctgcg gaccgatccc
     1021 aaatattacg ccaggacacc cgaggaactg atggctgcgg catcgcgcca ggccaagatc
     1081 atcgatggcc tcatgccgcg ctactttggc ctgctgccgc ggcttcccta tggggtgaag
     1141 gaaatcccgg cggaaaccgc cgagaccacg acgacggcct attacggtcc gggatctccc
     1201 caggccgggc ttgccggcaa ctacttcgtc aacacctcga aactgccgca gcgcccgctt
     1261 tacgagcttc ccgcgctgac cgttcacgaa gccgtgcccg gccatcacca ccagatcgca
     1321 ttgcagcagg aactgaacct gccggaattc cgccgcaaca tcgccttctt caccgccttc
     1381 accgaaggct gggggctcta taccgaatac cttggcgaag agatgggcat ctacgatacg
     1441 cccgaaaaga tgatgggccg cctgtcatac gaaatgtggc gggcgtgccg cctggtggtc
     1501 gataccggaa tgcacgccaa gggctggacc aaggctcagg cggtgcaatt catgcaggac
     1561 aacaccgcgc tttccgccgc gaatatcgag gccgaagtca accgctacat ctcctggccg
     1621 ggccaggcgc tgggctacaa gatcggcgag atcaagatcc gcgaactgcg cgccagggca
     1681 gaatcggaac tgggcgcgaa gttcgatctg cgccgcttcc acgatgcggt tctgggccag
     1741 ggatcggtcc cgctcgacgt gctcgaagcc cagattgctg attggattgc cgccgaaaag
     1801 cagcgttgat caggcgaaaa tcgcggcctt gcgcagcagt tgataagccg cgatcacctc
     1861 gcccagcttg gcctcgaacg agcggtcgcc gccgttgcga tcgggatgat agcgcctgac
     1921 cagcgccgaa tagcgcgcgc gcagatcgtg ccgggtggca tcgagcgaaa gccctagctg
     1981 atcgagtgcc ctgcgctcgt ccgggctgac cgccttgcca tcggcgcgct cggccgggcg
     2041 cctggtccgc gcccgcgccg cgatcgctt
//
LOCUS       DASUDV010000052         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_465592,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000052 DASUDV010000000
VERSION     DASUDV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465592"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..581
                     /gene="ybeY"
                     /locus_tag="VFV30_01060"
     CDS             84..581
                     /gene="ybeY"
                     /locus_tag="VFV30_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX4846706.1"
                     /translation="MDLETAIEADWPSPPDWEDLTARAAAALAGVAPELANPRLSVSV
                     LYADDAEVHALNREWRGKDKPTNVLSFPMLERADLLALAPEGPPEMLGDIALALETCA
                     REAADKGLSLEHHATHLLIHGLLHLAGHDHEISAQDARAMEQLEIKALALLGIADPYG
                     DHDEQ"
     gene            600..1499
                     /locus_tag="VFV30_01065"
     CDS             600..1499
                     /locus_tag="VFV30_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX4846707.1"
                     /translation="MPEPHSGEADSRSLIGRLLRRLKSLGEPDQSLRAQLEEAIDEHE
                     EDGGNSSGDGDLSPLERQMLRNLLHFSEHDADDVAIPRGEIIAIPRSASWQDVIDAFA
                     EHGHSRLPVYGETLDEVVGMMLIKDVFTYLAQGKAPKDWTQLMRQPLFVPQARNALDV
                     LNDMRASRTHLAVVVDEYSGTDGIITIEDLVEEITGEIEDEHDDAPTELLVQLEGGLW
                     EADARAELEDVGERVDARLGEIEEDVDTLGGLAFVLAGQVPPVGAVLSHDSGWTLEVV
                     AGDEKRVTRLRLHPPLSAEAEGD"
     gene            1503..2387
                     /locus_tag="VFV30_01070"
     CDS             1503..2387
                     /locus_tag="VFV30_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4846708.1"
                     /translation="MAIRRLPPLRAIEAFVRIVRLGSARAAADELGLSPSALSRRVAA
                     LEEFIGKRLFTRQHQAMKLTDEGHAFYNAVGPKFDELAAAVEGQVENSKVLRLHLGVL
                     PLFGSQRLFPRLPELRKAHPRLHIDIDTSPHLDHRVGDTLDAAIVLSKQPDPGFHAVR
                     LDHNRVYAFTSKALAEEIGTSPDQDTLSRQTFLIHTELPDSFVAWKRAIGMADLDPAA
                     VDHFDSGQLILEAAANGLGIAIMHDDHFHRSHDSRLAKLFEVEVESPYSYWFICRPHA
                     LEVRAVRIFHDWLVKAGL"
     gene            2384..3031
                     /locus_tag="VFV30_01075"
     CDS             2384..3031
                     /locus_tag="VFV30_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846709.1"
                     /translation="MKAFGALAALLLLGAAAPATKGPSLSCTGSLSPSEAAICADPEL
                     AAWDRAVAKIYRASVKTSDITPSDQRQWLSRRDECQADRACLLREYRDWPGFGVTAAG
                     VGTVFERTDTGRRDPASLTILPIHGDWYYFSLIALHQQTDDPGSTNDGIYSGVIRLER
                     GIGTYDEGPEEYSCRFRLEREGSGWGISSFGEDTKCGGLNVFVDGSYRPVRRARR"
     gene            3070..3732
                     /locus_tag="VFV30_01080"
     CDS             3070..3732
                     /locus_tag="VFV30_01080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4846710.1"
                     /translation="MKRRFVLAALPAMAIALTGATKPRKTLAKAPPAVPLEDIVRVEI
                     VTELGTIVADLFHKQAPITVKNFVRYADSGRYNGMSFYRSMKLDWGTPPNGLIQAGIR
                     DGAKLLPPIAHEPTNVTGIKHKRGTLSMARLAPGTARSDFSILMSDLEGFDAVPDSPD
                     PEKRAGYCAFGQVVSGMDVALKIFDAPRSPTLGEGFMKGQMLSPPVKVLRVRRVPRPA
                     EG"
BASE COUNT          666 a         1318 c         1264 g          597 t
ORIGIN      
        1 acgatcagga tttctatctt gatgatgcaa gactggtggc ggttgactat gcgaagccga
       61 tctgccggta agacaccaat ctgatggacc tcgaaaccgc aatcgaagcc gactggccct
      121 ccccgcccga ctgggaggac ctgaccgcgc gcgccgcggc ggcgctggcc ggggttgcgc
      181 cggaactggc caatccgcgg ctgtcggtct cggtcctcta tgccgacgat gccgaggtcc
      241 acgcgctcaa ccgcgaatgg cgcggcaagg acaagccgac caatgtcctg tcctttccga
      301 tgctggagcg cgcggacctg ctggcacttg ccccggaggg cccgccggaa atgctgggcg
      361 acattgccct ggcgctggaa acctgcgcgc gcgaagcggc cgacaagggc ctcagcctgg
      421 agcatcacgc aacgcacctg ctgatccacg gcctgctcca cctggccggg cacgatcacg
      481 agatttcggc gcaggatgcc cgcgccatgg aacagctgga aataaaggcc cttgcccttt
      541 tgggcattgc cgacccatat ggcgaccacg acgagcaata gggactgcga caaggggcta
      601 tgcccgaacc acattctgga gaagcggaca gtagaagcct gatcgggcga ctgctgcgcc
      661 gcttgaaaag cctgggcgag ccggaccaat cgctgcgcgc gcagctggaa gaggcgatcg
      721 acgagcacga ggaagacggc ggcaacagct ccggcgatgg cgacctttcc ccgcttgagc
      781 ggcaaatgct gcgcaacctg ctccatttca gcgaacacga tgccgatgac gtcgccatcc
      841 cgcgcggcga aatcatcgcg atcccgcgca gcgccagctg gcaggacgtg atcgatgcct
      901 tcgccgaaca cggccattcg cgcctgccgg tctatggcga gacgctggac gaagtggtcg
      961 gcatgatgct gatcaaggac gttttcacct atctggccca gggcaaggcg cccaaggact
     1021 ggacccagct gatgcgccag ccgctgttcg tcccccaggc ccgcaacgcg ctcgacgtgc
     1081 tgaacgacat gcgcgccagc cgcacccacc tggccgtggt ggttgatgaa tactccggca
     1141 ccgacgggat catcacgatc gaggacctgg tcgaggaaat caccggcgag atcgaggacg
     1201 agcacgacga cgcgccgacc gagctgctgg tccagctcga gggcggcctg tgggaagccg
     1261 atgcccgcgc cgaactggaa gacgtgggtg agcgggtcga cgcgcgcctt ggcgagatcg
     1321 aggaagacgt cgacaccctg ggcgggctgg cctttgtcct ggccgggcag gtcccgccgg
     1381 tcggcgccgt gctgtcccac gacagcggct ggacgctgga agtggtcgcc ggcgacgaga
     1441 agcgggtcac ccggctgcgg ctgcacccgc cgctttcggc cgaagccgag ggggactaga
     1501 ccatggccat tcgccggctc cctcccctgc gcgcgatcga ggcgtttgtg cggatcgtgc
     1561 ggctgggcag cgcccgggcc gcggcggacg agctgggcct ttcgcccagc gcgctctcgc
     1621 gccgggtggc cgcgctggaa gagttcatcg gcaagcggct gttcacgcgc cagcaccagg
     1681 cgatgaagct caccgacgaa ggccacgcgt tctacaacgc ggtcggcccc aagttcgacg
     1741 agctggccgc cgcggtcgaa ggccaggtgg aaaacagcaa ggtgctgcgc ctgcacctcg
     1801 gcgtcctgcc gctgttcggc agccagcggc tgttcccgcg cctgcccgaa ctgcgcaagg
     1861 cccacccgcg cctgcacatc gatatcgaca ccagcccgca cctcgatcac cgggtcggcg
     1921 atacgctgga tgccgcgatc gtcctgtcga agcagcccga tccgggcttt cacgcggtcc
     1981 ggctggacca caaccgggtc tatgccttca cctccaaggc cctggccgag gaaatcggca
     2041 ccagccccga tcaggacacg ctttcgcgcc agaccttcct gatccacacc gaactgccgg
     2101 acagcttcgt cgcctggaag cgcgcgatcg gcatggccga tctcgatccg gccgcggtgg
     2161 accacttcga ttccggccag ctgatcctcg aggccgccgc caatggtctg ggcatcgcga
     2221 tcatgcacga cgaccacttc caccgcagcc acgacagccg cctggccaag ctgttcgagg
     2281 tcgaggtcga aagcccctat tcctactggt tcatctgccg cccccacgcg ctggaagtcc
     2341 gcgcggtgcg gatcttccac gactggttgg tgaaggccgg gctgtgaagg cgtttggcgc
     2401 actcgccgcc ctgctgctgc tgggcgcagc ggcgccagcc acgaagggcc cgtccctttc
     2461 ctgcaccgga tcactctctc ccagcgaagc cgcgatctgc gccgatccgg aactggccgc
     2521 ctgggaccgg gcggtggcca agatctaccg ggcgagcgtc aagacctcgg acatcactcc
     2581 gtccgaccag cgccagtggc tgtcgcgccg cgacgaatgc caggccgacc gggcctgcct
     2641 gctgcgcgaa taccgcgact ggccgggctt cggcgtgacc gccgccgggg tcgggacggt
     2701 gttcgaacgc accgacaccg gtcggcgcga tccggcctcg ctgacaatcc tgccgatcca
     2761 cggcgactgg tattactttt ccctgattgc cctgcaccag cagaccgacg atcccggcag
     2821 caccaacgac gggatctatt ccggcgtcat ccggctggag cgcggaatcg gcacctacga
     2881 cgagggcccc gaggaatact cctgccggtt ccggctggag cgcgaagggt ccggctgggg
     2941 catttccagc tttggcgagg acaccaagtg cggcgggctc aacgtctttg tcgacggcag
     3001 ctaccgcccg gtccggcgcg cgcggcgtta aggctttccg ttggccgcca atgttgcatc
     3061 atggcggcca tgaaacgccg attcgtcctt gccgccctgc ccgccatggc catcgccctg
     3121 accggcgcaa ccaagccccg caagaccctg gccaaggctc cgcccgcggt gccgctggag
     3181 gacattgtcc gggtggagat cgtgaccgaa ctgggcacca tcgtcgccga cctgttccac
     3241 aagcaggcgc cgatcacggt caagaacttc gtccgctatg ccgacagcgg gcgctacaac
     3301 gggatgagct tttaccgctc gatgaagctc gattggggca cgccgcccaa cgggctgatc
     3361 caggccggga tccgcgacgg ggccaaactg ctgccgccga tcgcgcacga gccgaccaat
     3421 gtcaccggga tcaagcacaa gcgcggcacg ctttcgatgg cgcggctggc gccgggcacg
     3481 gcgcggtcgg acttttcgat cctgatgagc gatctggaag gctttgacgc cgtgccggat
     3541 tcgcccgatc cggaaaagcg cgcgggctat tgcgcgttcg gccaggtcgt ctcgggcatg
     3601 gacgtcgcgc tgaagatctt cgacgcgccg cgctcgccca cgctgggcga agggttcatg
     3661 aaaggccaga tgctgagccc gccggtcaag gtgctgcggg tgcggcgcgt tccccgcccg
     3721 gcagagggct agaaccgcgt ttcaagccgc gcacggttgg cggatgatgg aagggtttgg
     3781 gtggggatcc ccgttgcggc agggaccctg caatccatca tccgccgcac cgtatccggc
     3841 ccgcg
//
LOCUS       DASUDV010000053         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1813215,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000053 DASUDV010000000
VERSION     DASUDV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813215"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFV30_01085"
     CDS             <1..454
                     /locus_tag="VFV30_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4846711.1"
                     /translation="QVAINHKYMFPLYACAVELDIPICLNVGIPGPRIPMETQKVEHL
                     DEVCWFFPDLKIVMRHGAEPWQDLAVKLMLKWPNLYYSTSAFAPKHYPKAIIDYANTR
                     GAEKIIYAGYFPMGLSLERIMSDMENVPLKDEVWPKFLRDNAARVFKL"
     gene            495..1601
                     /locus_tag="VFV30_01090"
     CDS             495..1601
                     /locus_tag="VFV30_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX4846712.1"
                     /translation="MTEAATDARTAPVAVWQVLEKMTKEEREAWRVAPIANRPSAEAR
                     NLDIGFPFGWYPFMLARDLAVGEVKPVRYFARDLAVWRGEDGQVRMIDAYCKHLGAHL
                     GHGGKVAGNLLECPFHAWRYDGQEGVVKEIPYAKAIPPQVKRKCTRTWHIREANQWLW
                     VWYHPEDEAPLYEVVHLPEASDPDWTPYDIVEWKVFGSLQNMAENGVDFAHFKYIHGT
                     AELPSSELSWGEWDRCGIVRAKMGTPKGMVDGAITSNSVGPGQAWVRFTGICETLLVA
                     CIIPVELDELQVIYCYTQPREQAEGPMAGLARAIIKDVNKQLDQDKVVWDRMRYEPNP
                     IICDGDGPIPHFRKFYKRFYAGGDGVPSSQRQAG"
     gene            1605..3122
                     /locus_tag="VFV30_01095"
     CDS             1605..3122
                     /locus_tag="VFV30_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4846713.1"
                     /translation="MPTRRDVMAGGAAAVALGAAGGAQAMKAGGMDELLSRHDAVGLA
                     RLVRQRKVAPSELLEAAIARAEALNPRFNFMAQKHYDLGRAAIAKGLPKGPFTGVPLL
                     LKDLHTHIAGELSEGGSRLYKGYRAPVTSELVRRYQAAGFVIFGKTTTPEYGLTGTTE
                     NKLTGDTRNPWNPAHSSGGSSGGAAAAVAAGVIPAAHASDGGGSIRIPASCCGLFGMK
                     PSRGRVPMGPLRTEGWGGLSTNHAITRSVRDSAAMLDASHGIEPGSRYGAPSPAESFL
                     AQVGKHPGKLRIALMTRPISGSPVDSECSDAARAAAKLCESLGHHVEEARLELDAALL
                     GWASFVSIGPAVAADIADRAKVLGIVPGPDVVEPVVLAFAEFGKTFTAMDAARASGVL
                     QDAAIKVARFMERFDVILTPTLAAPPIALGRINLTPDCGFQEWGQRAAVYSPFCQLAN
                     LTGQPAMSVPLAQAKSGLPIGVMFTGRYGDEALLYRLAGQLEKALPWAGKRPQIA"
     gene            3147..3440
                     /locus_tag="VFV30_01100"
     CDS             3147..3440
                     /locus_tag="VFV30_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846714.1"
                     /translation="MLETIAPFVGLLGLIGLAGLAGIKNPVAKDRAGAAVRLLGILGL
                     AGFAGIWIDGAGAMGAAGALGLWNHQVPKLARWGRLGWVFPAGIPYLVGHFLG"
     gene            complement(3437..4867)
                     /locus_tag="VFV30_01105"
     CDS             complement(3437..4867)
                     /locus_tag="VFV30_01105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4846715.1"
                     /translation="MRIGRRTLLAGTAAVAAGAAGAWWWGGEPPVPKADRAARTEGQL
                     FERGLIGIERSGWTRPEGVAAPWPVLAADLAADVLVVGAGLAGSSLALHLAEAGLKVA
                     VIEARQPGWGASGRNAGHVLPTLRDAAVFERFPDGGKAFLDAFRKHRNLPYDLAARHG
                     AAQDAVRSGYLNVAENAEAAAEFRAKSAWMERQGLLRIEETGGEELVRQTGSRLWTHA
                     ISFPDGGQVNPYRLTNAMTAAAARLGALVFGESPAIRIDQAGSRWTVRTPRGSVTAER
                     VVFCTNAYPTDVVPEFATAFYPLTAYALTTRTLPDEAKALIMPGRQTLSQLPLDINPL
                     VRDRHDRLILSSIPTVSSPEDAVWHFRNQLAWLHHAWPETKAMKIELEAYWTGRVAMR
                     DREFPGVFQPRPGIYGLMYFNAWGNLMAPLMGKLLARGLAADRTDALPFPIEKPLAVS
                     NQGKMDRIIRHLLIPAARTAQNLGVL"
BASE COUNT          852 a         1683 c         1760 g          812 t
ORIGIN      
        1 gcaggtggcg atcaaccaca agtacatgtt cccgctctac gcctgcgcgg tggaactgga
       61 cattccgatc tgcctcaacg tcggcattcc cggcccgcgc atcccgatgg aaacgcaaaa
      121 ggtcgagcat cttgacgagg tctgctggtt cttcccggac ctcaagatcg tgatgcgcca
      181 cggggccgag ccgtggcagg acctggccgt caagctgatg ctcaaatggc cgaacctcta
      241 ttattcgacc agcgccttcg cccccaagca ctacccaaag gcgatcatcg attacgccaa
      301 cacccgcggc gcggagaaga tcatctacgc cggctatttc ccgatgggcc tcagcctgga
      361 gcggatcatg tccgacatgg agaacgtgcc cctgaaggac gaggtctggc ccaagttcct
      421 gcgcgacaac gccgcgcgcg tcttcaagct ctgaccgaca cgacaaccag aaccggatca
      481 ccggggagaa caccatgacc gaagccgcca ccgatgcacg caccgcgcct gtcgcggtct
      541 ggcaggtcct tgaaaagatg accaaggaag agcgcgaggc ctggcgcgtc gcgccgatcg
      601 ccaaccgccc cagcgcagag gcgcgcaacc tcgacatcgg gttcccgttc ggctggtatc
      661 cgttcatgct ggcccgcgat ctggccgtgg gcgaggtcaa gccggtccgc tatttcgcgc
      721 gcgacctggc cgtgtggcgc ggcgaggacg ggcaggtccg gatgatcgac gcctattgca
      781 agcacctggg cgcgcacctg ggtcacggcg gcaaggtggc tggcaacctg ctggaatgcc
      841 cgttccacgc ctggcgctat gacggtcagg aaggcgtggt gaaggagatc ccctatgcca
      901 aggcgatccc cccgcaggtg aagcgcaagt gcacccgcac ctggcacatc cgcgaggcga
      961 accagtggct gtgggtgtgg tatcacccgg aagacgaagc tccgctttac gaagtggtcc
     1021 acctgcccga ggcgagcgat ccggactgga cgccctatga catcgtcgaa tggaaggtgt
     1081 tcggctcgct ccagaacatg gccgaaaacg gcgtggactt tgcccacttc aagtacatcc
     1141 acggcacggc cgagctgcca agttccgaac tgtcgtgggg cgagtgggac cgctgcggga
     1201 tcgtccgcgc caagatgggc acgcccaagg gcatggtcga tggcgcaatc accagcaact
     1261 cggtgggtcc gggccaggcc tgggtccgct tcaccggcat ctgcgagacg ctgctggtag
     1321 cctgcatcat cccggtcgag ctggacgagc tgcaggtgat ctattgctac acccagccgc
     1381 gcgagcaggc cgaagggccg atggcggggc tggcccgggc gatcatcaag gacgtcaaca
     1441 agcagctcga tcaggacaag gtggtgtggg accggatgcg gtatgaaccc aacccgatca
     1501 tctgcgacgg cgacgggccg atcccgcatt tccgcaagtt ctacaagcgc ttctacgccg
     1561 gcggtgacgg cgtgccgtca tcccagcggc aggcaggctg agccgtgccg acccggcgcg
     1621 acgtgatggc gggcggggcg gccgcagtcg cgctgggcgc agcaggagga gcacaggcga
     1681 tgaaggccgg cggaatggat gagctgctgt cgcgccacga cgcggtcggg ctggcccggc
     1741 tggtccgcca gcgcaaggtc gcgcccagcg aactgctcga ggcggccatc gcccgggccg
     1801 aggcgctcaa cccgcggttc aatttcatgg cgcagaagca ctatgacctg ggccgcgccg
     1861 ccattgccaa gggcctgccc aaggggccgt tcacgggcgt cccgctgctg ctcaaggacc
     1921 tgcacaccca tatcgcgggt gaactgagcg aaggcggcag ccgcctctac aagggctacc
     1981 gcgcgccggt gacgtccgaa ctggtgcggc ggtatcaggc cgcggggttt gtcatcttcg
     2041 gcaagaccac cacgcccgaa tacggcctga ccggcacgac cgagaacaag ctgaccggcg
     2101 atacccgcaa cccgtggaac ccggcgcaca gttcgggcgg atcctcgggc ggggcggcgg
     2161 cagcggtggc ggccggggtg atcccggcgg cccacgccag cgacgggggc ggctcgatcc
     2221 gcattccggc ctcgtgctgc gggctgttcg ggatgaagcc gagccgcggg cgggtgccga
     2281 tggggccgct gcgcaccgag ggctggggcg ggctttccac caaccacgcg atcacccgca
     2341 gcgtgcggga ttctgccgcc atgctggatg ccagccacgg gattgagccc ggctcgcgct
     2401 atggcgcgcc gagcccggcc gagagcttcc tggcccaggt cggcaagcac cccggcaagc
     2461 tgcggatcgc gctgatgacc cgcccgatct ccggctctcc ggtcgacagc gaatgcagcg
     2521 atgcggcacg ggcagcggct aaactctgcg agagccttgg ccaccatgtc gaggaagccc
     2581 ggctggagct ggatgcggcg ctgctcggct gggccagctt cgtttcgatc ggcccggcgg
     2641 tggcggcgga cattgccgac cgggccaagg tccttggcat cgtgccgggg ccggatgtgg
     2701 tcgaaccggt ggtgctggcc ttcgccgaat tcggcaagac cttcaccgcg atggatgcgg
     2761 cccgggccag cggcgtgctg caggacgccg cgatcaaggt cgcccggttc atggaacgct
     2821 tcgacgtgat cctgaccccg acgctggccg ccccgccgat cgcgctgggg cggatcaacc
     2881 tgacgccgga ctgcggtttt caggaatggg gccagcgcgc ggcggtctac tcgccgttct
     2941 gccagctggc caacctgacc gggcagccgg ccatgtcggt gccgctcgcc caggcgaagt
     3001 ctggcctgcc gatcggggtg atgttcaccg gccgctatgg cgacgaggcg ctgctttacc
     3061 gtcttgccgg acaacttgag aaagccctgc cctgggccgg aaaacggccg cagattgcct
     3121 gacggggatc gtggagggga atgcacatgc ttgagacaat tgcgccattt gtcggccttc
     3181 tgggtctgat cggcctcgcg ggcctggcgg ggatcaagaa ccccgtcgcc aaggaccgcg
     3241 ccggagcggc ggtgcggctg ctgggcatcc ttggccttgc cggcttcgcc ggcatctgga
     3301 tcgacggggc gggcgcgatg ggcgcggccg gggcgctcgg cctgtggaac caccaggtac
     3361 cgaagctggc ccgctggggc cggctgggat gggtcttccc ggccgggatc ccgtacctcg
     3421 tggggcactt tctcggttaa agtacgccca ggttctgcgc ggtgcgcgcc gccgggatca
     3481 gcaggtggcg gatgatccgg tccatcttgc cctggttcga tacggccagc ggcttctcga
     3541 tcgggaaggg cagggcgtcg gtgcggtcgg ctgccaggcc cctggccagg agcttgccca
     3601 tcagcggcgc catcaggttg ccccaggcgt tgaagtacat caggccgtag atgcccgggc
     3661 gtggctggaa cacgccggga aattcccggt cgcgcattgc cacgcgcccg gtccagtagg
     3721 cttccagctc gatcttcatg gctttcgttt ccggccaggc atggtgcagc caggccagtt
     3781 ggttgcggaa atgccagacg gcgtcttcgg gtgacgacac ggtcgggatc gatgacagga
     3841 taagccggtc atgccggtcg cggaccagcg gattgatgtc gagcggcagc tggctgagcg
     3901 tctggcgacc gggcatgatc aatgccttcg cctcgtcagg caacgtccgg gtggtcaggg
     3961 cataggcggt cagcggatag aaggcggtgg cgaattccgg caccacatcg gtcggatagg
     4021 cattggtgca gaacaccacc cgttcggcgg tgacgctgcc gcgcggggtg cgcaccgtcc
     4081 agcgcgaacc ggcttggtcg atccggatcg ccgggctctc gccaaacacc agcgcgccca
     4141 gccgcgctgc cgcggcggtc atggcgttgg tcagccgata gggattgacc tggccgccat
     4201 cggggaagga aatcgcgtgg gtccacaacc tgctgccggt ctgccggacc agctcctcgc
     4261 cgccggtctc ctcgatccgc agcaggccct gccgctccat ccaggccgat ttggcgcgga
     4321 attcggccgc ggcctcggca ttctcggcga cgttgagata gcccgaacgg accgcatcct
     4381 gcgccgcgcc atgccgcgcg gcaagatcat agggcaggtt gcggtgtttg cggaaagcat
     4441 cgaggaaggc tttgccgcca tctgggaagc gttcgaacac ggccgcatcg cgcagggttg
     4501 gcagcacgtg cccggcattg cgccccgaag cgccccagcc cggctggcgg gcctcgatca
     4561 cggcgacctt caggccagct tcggccaggt gcagcgccag cgacgatccg gccagccccg
     4621 cgcccaccac cagcacgtcg gcggcaagat cggcggccag caccggccag ggcgctgcca
     4681 cgccttcggg ccgggtccag ccgctccgtt cgatcccgat caggccgcgt tcgaacagct
     4741 ggccctcggt ccgtgcggcg cggtcggcct tcggcacggg cggttcaccg ccccaccacc
     4801 aggccccggc ggcacctgcg gcaacggcgg cagtgccggc cagcagggtt cgtctaccaa
     4861 tccgcattgt acccgttcct tccatccccg gcccatccgc ccccttgcgc cgggcggcct
     4921 gggtcgacat cctctcgatc cgacccttga ctggtcctaa atacgcaaaa agtaaattga
     4981 actttggttt tgacagcagt ttgcgtatga aatagccatt attgaaacgc aaagtcatgg
     5041 agagtgtcat gcgtggtttg aagggcaagg tcggtgtcgt tgccgggggc gggcgcggaa
     5101 tcggtgc
//
LOCUS       DASUDV010000054         8798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2166749,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000054 DASUDV010000000
VERSION     DASUDV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166749"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFV30_01110"
     CDS             <1..397
                     /locus_tag="VFV30_01110"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065909.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX4846716.1"
                     /translation="SVAKAKGLTPVATVVAVAAHAQEPKDFTTAPVGAINKVLAKAGW
                     SLADVDLFEVNEAFACVAMFAMHDLGIPHEKINVHGGATALGHPIGASGARIVTTLIA
                     ALQRHGKTKGVASLCIGGGEATAVAIQLV"
     gene            540..806
                     /locus_tag="VFV30_01115"
     CDS             540..806
                     /locus_tag="VFV30_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846717.1"
                     /translation="MNTKLIAPALIAGALIAGLALPAAAQARVTRAELRHDVQQVKEE
                     RRDVREEVRERDRAGVREEKRELNRAEGELRRDTRRFVRQRTRH"
     gene            complement(835..1344)
                     /locus_tag="VFV30_01120"
     CDS             complement(835..1344)
                     /locus_tag="VFV30_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX4846718.1"
                     /translation="MKRFAFPRYQRLMPFPRRVLAVFALLFLAVPALGQDSVKTPYWA
                     SIRASVLNMRVGPGEDYKILWVYRRPTLPLKVLRIKEGWRYVQDPDGAQGWVLHSLLT
                     RQRTGYIQGKQPADMRASGDAGAKLLWRLAPGVIGFLGDCEQGWCAFTVGPRKGFVPQ
                     DRIWGAGEP"
     gene            1386..2405
                     /locus_tag="VFV30_01125"
     CDS             1386..2405
                     /locus_tag="VFV30_01125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX4846719.1"
                     /translation="MPIVTDSPLTRRVEGKPRVHVTRHLLPSVEARMGELFDVVLNVE
                     DRPLTRDELIAAMRESDVLVPTVTDRIDAAMLEAAGDRLGLIANFGAGIEHIDLSAAR
                     ARKIMVTNTPGVFTDDTADLTMMLILSVPRRLGEGSRLIRDGKWTGWAPTAMLGRCVG
                     GKRLGIVGMGRIGQAVAHRARAFGMDVVYHNRHRLPPSVETMFGVRYEPDLEALFAEA
                     DVLTLHCPAGSSTHHMVNAARLATMEPTAYLINTARGDLIDEEALIAALQNGTIAGAG
                     LDVFAREPQVDPRLIALPNVITLPHLGSATIEGRAHAGEKVIANIRFWTDGHRPPDQV
                     LEGWA"
     gene            2524..3843
                     /locus_tag="VFV30_01130"
     CDS             2524..3843
                     /locus_tag="VFV30_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX4846720.1"
                     /translation="MRFFPALSLAALAALVPAAAHAQAEVAEVTGDALNTGDTAWVLV
                     SAALVLLMTMPGLTLFYGGLVRAKNFLSVLVQVGAVAAAASVLWIMVGYTLAFSTGST
                     GWIGGFDMAMLNGTIDVLHGETSISVRTNALFQMTFAAITPALMVGAWVDRARFGWVV
                     AFCALWGLVVYVPVAHWVWGGGWLATSIGTKDFAGGIVVHTTAGVSALVAAALVGKRQ
                     GFPKQLMLPHSPSLTMAGAALLWVGWFGFNGGSALAADDSATSAIIATHLGASMAALT
                     WLLIERIKVGKATSVGFATGAIAGLATVTPAAGFISPGAAIVIGVVAALVCFPMIQIV
                     KKKLEIDDSLDVFAVHGVGGITGSLLLAVFLEPALGGIGYDEGTTLISQFAAQAVGVG
                     AVAIWSALATALLALLVGLVFPMRVGEDDEREGLDLASHGERAWEMD"
     gene            3853..4347
                     /locus_tag="VFV30_01135"
     CDS             3853..4347
                     /locus_tag="VFV30_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX4846721.1"
                     /translation="MEGGRPAWLSTLLSAIRGTFRPGGRALFSELAIYWLVAILLKMA
                     LAIPLAVVLSEQVELRRQIDDALTLVFVLPLPALLARRLHDQNRSALYLLLAAPGIAL
                     EAARKAVSLSQGIEARVRFDAATWPLDVLATVAGIALLLLMFFPGTHGPNRFGPDPRG
                     TAPV"
     gene            complement(4344..5750)
                     /locus_tag="VFV30_01140"
     CDS             complement(4344..5750)
                     /locus_tag="VFV30_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX4846722.1"
                     /translation="MSFKGLKPILYGGREVWPLVEGGKGVAVSNHASSGAWAAAGGIG
                     TVSAVNADFYDENGNICDQPYFGATRKDRHDELVAYGIKGGVEQVRRAYEISGGVGAI
                     NINVLWEMGGAQQVLEGVLEKTRGMVTGVTCGAGMPYKLSEIAARFNVNYLPIISSAR
                     AFRALWKRAYHKVADLMGAVVYEDPWLAGGHNGLSNAEDPRVPQDPYPRVKALRDTMR
                     AEGIADSVPIVMAGGVWYLRDWENWIDNPELGQIVFQYGTRPLLTQESPIPQDWKDHL
                     RTLEPGDILLHRFSPTGFYSSAVRNPFLRNLEARSARQIPYSKVEAGEHTARLDVGVG
                     NKNFWVAPEDLARARAWVAQGFTEGLRTPDDTIVFVDPAERSRIQTDQKECMGCLSHC
                     GFSSWKDHDDYTTGYLADPRSFCIQKTLKAIAHGKDVHNNLMFAGHAAHNFRTDPFYS
                     NNFTPTVKQLVDRILTGD"
     gene            complement(5747..6454)
                     /locus_tag="VFV30_01145"
     CDS             complement(5747..6454)
                     /locus_tag="VFV30_01145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4846723.1"
                     /translation="MAATLAGCNRPAGDTDRPEGAREFPRPYRPVSAITGNEFSNEDQ
                     RDDLGEARVVMDLAQIGAGTTVADIGAGEGYYTIRLAERVGPKGRVLAQDIDAKVIQR
                     LGQRVERERLDNVSIKLGDADDPKLPHRSFDRVFMVHMYHEVTEPYAFLWRLRPALKP
                     GGQVVVVDVDRPTDQHGIPPALLFCEFAAVGFRLVEFVRKPELNGYYASFEAVGEPPK
                     PQDIKPCNTANGKTGTQ"
     gene            complement(6481..7608)
                     /gene="prfB"
                     /locus_tag="VFV30_01150"
     CDS             complement(6481..7608)
                     /gene="prfB"
                     /locus_tag="VFV30_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX4846724.1"
                     /translation="MRAEGQAHIDRIEAALALVRKFLDWDRALRRLDELNARVEDPKL
                     WDNPKEAEAVMKERRRLEAAVGTVNDISAKMADAIEFVELGEMEDDADTVDEGIAALA
                     ALADRADADKVQALLGGEADSNDTFIEIHAGAGGTESQDWAEMLLRMYTRWAEKRGFK
                     VELIEHQSGEQAGIKSATILCKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSV
                     WVYPVIDDSFQIDINPADLKIDTYRASGAGGQHVNTTDSAVRITHQPSGIVVASQIDR
                     SQHKNREIAMGMLKARLYEEEMRKREEAASAEHAAKTDIGWGHQIRSYVLQPYQMVKD
                     LRTGVTSPTPSDVLDGALDPFMAAALAQRVTGEKVDVEDVD"
     gene            complement(7694..8239)
                     /locus_tag="VFV30_01155"
     CDS             complement(7694..8239)
                     /locus_tag="VFV30_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4846725.1"
                     /translation="MKRTLFAALAAASVFALPASAPADLPKGARAPQIVAQGALAGKV
                     FTFNLEQALKKGPVVLYFYPKAFTQGCTFEARAFAEASAEFRAAGATVVGMSADDLPS
                     LQRFSTEACRDKFAVAIASPQTIAAYDVVLKKADGSATGLSTRTSYVIGKDRRIKFVY
                     TNMDYREHVKQTLQAVKALRR"
     gene            complement(8281..>8798)
                     /locus_tag="VFV30_01160"
     CDS             complement(8281..>8798)
                     /locus_tag="VFV30_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX4846726.1"
                     /translation="GLPLFGKTGTTTGPTNVWFVGGNQEIVGGVYLGYDQPASLGGYA
                     QGGTIAAPIFKQMVQATKPRWSRRPFTAPKGIHWVRVDRISGKRVLDGMPGSDPKSSI
                     IWEAFKAESEARTGPSTEALAAQRDALLKSLRRGATRKDQPAGAEGDPGLATEPKAAP
                     TIAADPGVAGQ"
BASE COUNT         1351 a         2991 c         2944 g         1512 t
ORIGIN      
        1 cagcgtggcc aaggccaagg gcctgacccc ggtcgccacg gtcgtcgcgg tcgcggctca
       61 cgcgcaggag ccgaaggact tcaccaccgc cccggtcggc gcgatcaaca aggtgctcgc
      121 caaggccggc tggagcctgg ccgatgtcga cctgttcgag gtcaacgagg ccttcgcctg
      181 cgtcgccatg ttcgcgatgc acgacctggg catcccgcac gagaagatca acgtccacgg
      241 cggggcgacc gcgctgggcc atccgatcgg ggcctcgggc gcgcggatcg tcaccaccct
      301 gatcgcggcg ctgcagcgcc acggcaagac caagggcgtc gccagcctgt gcatcggcgg
      361 cggcgaagcc actgccgtgg cgatccaact ggtctgatcc cacagcgaca attcgcgaca
      421 ttccggcagg gaaatcttac atttggtttc cctgccgttc agccggccgc gccgaatttc
      481 gggctcgcgg ccgggcaatc ccgcctgggc cgccatccgc aacccaacgg gagaagccca
      541 tgaacaccaa gctgattgcc cccgccctga tcgccggcgc cctgatcgcc ggcctggccc
      601 tgccggccgc cgcccaggcc cgcgtcaccc gcgccgagct gcgccatgac gtccagcagg
      661 tgaaggaaga gcgccgcgac gtccgcgagg aagtgcgcga gcgcgaccgc gccggcgtgc
      721 gcgaggaaaa gcgcgaactg aaccgggccg aaggcgaact gcgccgcgac acgcgccggt
      781 tcgtccggca gcgcacccgc cactaaagct ggattggacg caggggcggc ccgctcaggg
      841 ctcccctgcc ccccagatcc ggtcctgcgg gacaaagccc ttgcgcgggc cgacggtgaa
      901 ggcgcaccag ccctgctcgc aatcccccag gaagccgatc acgccgggag ccaggcgcca
      961 cagcagcttg gccccggcat cgccggaggc gcgcatgtcg gcgggctgct tgccctggat
     1021 atagccggtg cgctggcggg tcagcaggct gtgcaggacc cagccctgcg ccccatcggg
     1081 atcctggaca tagcgccagc cttccttgat ccgcagcacc ttcagcggca gcgtcggccg
     1141 ccggtatacc cacaggatct tgtagtcctc tcccggaccg acccgcatgt tgaggaccga
     1201 cgcccggatc gaggcccaat agggagtctt caccgaatcc tgccccaggg cgggaaccgc
     1261 cagaaacagc agggcaaaca cggcgaggac acggcgggga aagggcatga gtcgctgata
     1321 gcgcgggaag gcgaaccgct tcaagccgct tgaccgccat gcccgccccg gcctagcgca
     1381 agggcatgcc gatcgtaacc gacagtccgc ttacccgccg cgtcgaaggc aagccccggg
     1441 tgcacgtaac ccgccacctg ctgccttcgg ttgaagcccg catgggcgaa ctgttcgatg
     1501 tcgtcctcaa cgtcgaggat cgccccctga cgcgcgatga gctgatcgcc gcgatgcggg
     1561 aaagcgacgt gctggttccg accgtgaccg accggatcga cgccgccatg ctggaggccg
     1621 ccggtgaccg gctgggcctg atcgccaatt tcggcgccgg gatcgaacat atcgatcttt
     1681 ctgccgcccg ggcccgcaag atcatggtca ccaacacgcc gggcgttttc acggacgata
     1741 ccgccgacct gacgatgatg ctgatcctgt cggtcccgcg gcggcttggc gaaggcagcc
     1801 gcctgatccg cgacggcaag tggaccggct gggcccccac cgcgatgctg ggccgctgcg
     1861 tcggcggcaa gcggctgggg atcgtcggca tgggccggat cggccaggcc gtggcccacc
     1921 gcgcccgggc ctttggcatg gacgtggtct atcacaaccg ccaccgcctg ccgcccagtg
     1981 tcgagaccat gttcggggtg cgctacgaac cggatcttga ggcgctgttt gccgaagccg
     2041 acgtgctgac cctgcactgc ccggcgggca gcagcacgca ccacatggtc aatgccgcgc
     2101 ggctcgccac gatggagccg accgcctatc tgatcaacac cgcgcgcggc gatctgatcg
     2161 acgaggaggc gctgatcgcg gccctgcaga acggcacgat cgccggggcg ggcctggatg
     2221 tctttgcccg cgagccgcag gtcgatccgc ggctgattgc gctccccaac gtgatcaccc
     2281 tgccccatct gggcagcgcc acgatcgagg gacgggccca cgccggggaa aaggtgatcg
     2341 ccaacatccg gttctggacc gacgggcacc gcccgcccga tcaggttctg gaaggctggg
     2401 cataagcgcc ccttcgcgca acaaggcttc acgctgcgcc ttgccgaata tgcaatcgtt
     2461 gctttaagcc gggcctgaca ggaatcacgc gcgcgcccaa gccgcgcccg aggggagcta
     2521 ccgatgcgat ttttcccggc cttgagcctt gccgcgctgg cggcgcttgt tccggctgcg
     2581 gcccatgccc aggccgaggt ggccgaagtg acgggcgatg ctctgaacac cggcgatacg
     2641 gcctgggtgc tggtttcggc ggcgctggtg ctgctgatga ccatgcctgg cctgacgctg
     2701 ttctacggcg ggctggtgcg ggccaagaac ttcctgtcgg tgctggtcca ggtcggcgcg
     2761 gtcgcggcgg cggcttcggt gctgtggatc atggtcggct ataccctcgc cttcagcacc
     2821 ggctcgaccg ggtggatcgg cgggttcgac atggcgatgc tgaacggcac gatcgatgtg
     2881 ctgcacggcg aaaccagcat ctccgtgcgc accaacgcgc tgttccagat gacgttcgca
     2941 gcgatcactc cggccctgat ggtcggcgcc tgggtcgatc gcgcccggtt cggctgggtc
     3001 gtggccttct gcgccctgtg ggggctggtg gtctatgtgc ccgtcgccca ctgggtctgg
     3061 ggcggcggct ggctcgccac ctcgatcgga accaaggatt ttgccggcgg catcgtggtt
     3121 cacaccacgg ccggtgtctc ggcgctggtt gcggccgcgc tggtcggcaa gcggcagggc
     3181 tttcccaagc agctcatgct cccgcacagc ccctcgctga ccatggccgg ggccgcgctg
     3241 ctgtgggtgg gctggttcgg gttcaacggc ggttcggccc tggcggccga tgacagcgca
     3301 accagcgcga tcatcgccac gcacctgggc gccagcatgg cggcgctgac ctggctgctg
     3361 atcgaacgga tcaaagtggg caaggccacc agcgtgggct ttgccaccgg ggcgatcgcc
     3421 ggcctggcca cggttacccc ggccgccggt ttcatctcgc cgggggccgc catcgtcatc
     3481 ggcgtggttg ccgcgctggt ctgcttcccg atgatccaga tcgtgaagaa gaagttggaa
     3541 atcgacgatt cgctcgatgt gttcgcggtc cacggcgtgg gcgggatcac cggctcgctc
     3601 ctgctggcgg tgttcctgga gccggccctt ggcggcatcg gctatgacga gggcacgacc
     3661 ctgatcagcc agtttgcggc ccaggccgtg ggcgtcggcg cggtcgcgat ctggtccgcg
     3721 ttggccacgg ccctgctggc cctgctggtc ggcctggtct tcccgatgcg ggtgggcgag
     3781 gacgacgagc gcgagggcct cgacctcgcc agccacggcg agcgcgcctg ggaaatggac
     3841 tgagccgcgg cagtggaagg gggccgtcca gcctggctgt ccaccctcct ctccgcgatc
     3901 cggggcacct tccgcccggg cgggcgcgcc ctgttctcgg aactggcgat ctattggctg
     3961 gtggccatcc tgctcaagat ggcgctggcc attccgctgg cggttgtctt gtcggaacag
     4021 gtcgagctgc gccgccagat cgatgacgcc ctgaccctgg tgttcgtgct gccgctcccg
     4081 gcgttgctgg cccggcggct gcacgaccag aaccgttcgg ccctctacct gctgctggcg
     4141 gctccgggca tagcgctgga ggccgcccgc aaggccgtat ccctcagcca ggggatcgag
     4201 gcccgcgtgc gcttcgacgc cgcaacctgg ccgcttgacg tccttgcgac cgtggccggc
     4261 atcgcgctgc tgctgctgat gttctttccc ggcacccacg ggccgaaccg cttcggtccg
     4321 gacccgcggg gaaccgctcc ggtttaatcc ccggtcagga tccggtcgac cagctgcttc
     4381 accgtggggg tgaagttgtt ggaatagaac gggtcggtcc ggaagttgtg cgcggcgtgg
     4441 ccagcgaaca tcagattgtt gtgcacgtcc ttgccgtggg caatcgcctt cagcgtcttc
     4501 tggatgcaga agctgcgcgg atcggcgagg tagccggtgg tgtaatcgtc atggtccttc
     4561 cacgacgaga acccgcagtg cgacaggcag cccatgcatt ccttctggtc cgtctggatc
     4621 cggctgcgtt cggccggatc gacgaagacg atcgtgtcgt ccggcgtgcg caggccctcg
     4681 gtaaagccct gcgcgaccca ggcgcgggcc cgggcgaggt cttcgggcgc aacccagaag
     4741 ttcttgttgc cgacgcccac gtcaagccgg gcggtgtgct cgccggcttc gaccttggaa
     4801 taggggatct gccgcgcgct gcgggcttcg agattgcgca ggaaggggtt gcgcacggcc
     4861 gagctgtaaa agccggtcgg cgagaaacgg tgcagcagga tgtcgcccgg ctccagcgtg
     4921 cgcaggtggt ccttccagtc ctgcgggatc gggctttcct gagtcagcag cggacgggtg
     4981 ccgtactgga acacgatctg gcccagttcc ggattgtcga tccagttttc ccagtcgcgc
     5041 aggtaccaga ccccgccggc catcacgatc ggcaccgaat ccgcgatccc ttcggcgcgc
     5101 atcgtgtcgc gcagggcctt gacccgggga tagggatcct gcggcacgcg cggatcctcg
     5161 gcattggaca ggccgttgtg cccgccggcc agccacgggt cttcatagac caccgcgccc
     5221 atcaggtcgg cgaccttgtg ataggcccgc ttccacagcg cgcgaaaggc gcgggccgag
     5281 ctgatgatcg gcagatagtt gacgttgaac cgggccgcga tctcggacag cttgtagggc
     5341 atgccggcgc cgcaggtgac cccggtcacc atgccgcgcg tcttttccag cacgccttcc
     5401 agcacttgct gggccccgcc catttcccac agcacgttga tgttgatcgc gcccacgccg
     5461 ccggaaatct cataggcccg gcggacctgc tccaccccgc ctttgatccc gtaggcgacc
     5521 agttcgtcgt gccggtcctt gcgggtggcg ccgaaatagg gctgatcgca gatgttgccg
     5581 ttttcatcat agaagtcggc gttgacggcg ctgaccgtgc cgattccccc ggcagccgcc
     5641 caggcgccgg agctcgcgtg gttcgagacg gcaaccccct tgcctccctc gaccagcggc
     5701 caaacctcac ggccaccata aagaatcggc ttcaggccct tgaaactcat tgcgtccccg
     5761 tcttcccatt cgccgtgttg cacggcttga tatcttgtgg cttgggcggt tccccgaccg
     5821 cttcgaacga tgcgtaatag ccgttcaatt ccggcttacg gacaaattcg accaggcgaa
     5881 agcccacggc cgcaaattcg cagaacaaca gcgccggcgg gatgccgtgc tggtcggtcg
     5941 ggcggtcgac atcgaccacc acaacctgcc cgccgggctt cagcgcgggg cgcagccgcc
     6001 acaggaaagc ataaggctcg gtcacttcgt ggtacatgtg gaccatgaag acccggtcaa
     6061 agctgcgatg cggcagcttg ggatcgtcgg catcgcccag cttgatcgaa acgttgtcaa
     6121 gccgctcgcg ctccacccgc tggcccagcc gctggatcac cttggcgtcg atatcctgcg
     6181 ccagaacgcg gcccttgggg ccaacccgtt cggccaggcg gatggtgtaa tagccctcgc
     6241 ccgcgccgat atcggccacg gtggtgccgg cgccgatctg ggccaggtcc atcaccaccc
     6301 gggcctcgcc caggtcgtcg cgctggtcct cgttcgaaaa ctcgttgccg gtgatcgccg
     6361 agaccggccg gtagggccgc ggaaactcgc gcgcgccttc gggccggtcg gtatcacccg
     6421 ccggacggtt gcagcccgcc agtgttgcgg ccaccaggac cagggcgagc gcgccgcgga
     6481 tcaatcgacg tcctccacgt ccaccttttc tccggtgacg cgttgggcca gagccgcggc
     6541 catgaaggga tcgagcgcgc cgtccagcac gtcggacggg gtcgggctgg tcacgccggt
     6601 gcgcaggtcc ttcaccatct ggtacggctg cagcacatag gaccggatct ggtggcccca
     6661 gccgatatcg gtcttggcgg cgtgctcggc actggcggct tcctcgcgct tgcgcatttc
     6721 ctcttcatag aggcgcgcct tgagcatgcc catcgcgatt tcgcggttct tgtgctggct
     6781 gcggtcgatc tggctggcca ccacgatgcc ggacggctgg tgggtgatgc gcaccgcgga
     6841 atcggtggtg ttgacgtgct gcccgcccgc gcccgaagcg cggtaggtat caatcttgag
     6901 atcggccggg ttgatatcga tctggaagct gtcgtcgatc accggataga cccagaccga
     6961 gctgaagctg gtgtggcggc gggccgaact gtcatagggc gaaatgcgga ccaggcggtg
     7021 gacgccgctt tcggtcttgg catagccata ggcgttctcg cccttgcaca ggatcgtggc
     7081 cgacttgatt ccggcctgct cgcccgactg gtgctcgatc agttccacct tgaagccgcg
     7141 cttttcggcc cagcgggtgt acatccgcag cagcatttcg gcccagtcct ggctttccgt
     7201 gccgccggcc ccggcgtgaa tttcgatgaa ggtgtcgttg ctgtcggcct ctccgcccag
     7261 caaggcctga accttgtcgg catcggcccg gtcggccagc gcggcgagcg cggcgatgcc
     7321 ctcgtccacg gtgtccgcgt cgtcttccat ctcgcccagt tcaacgaact cgatcgcgtc
     7381 ggccatcttg gcggaaatgt cgttgacggt gccaaccgcg gcttccagcc ggcggcgctc
     7441 cttcatgacg gcttccgctt ccttggggtt gtcccaaagc ttcgggtcct cgacccgggc
     7501 gttcagctcg tcgaggcgcc gcaaggcgcg atcccagtcg agaaacttgc ggaccagcgc
     7561 cagcgcggct tcgatccggt caatatgagc ctgcccttcg gcacgcatgg cacggtaact
     7621 ccggtaatgc gaacaggcgc cttctggcgc gtcggccccg cttagcgaaa ggcctctgat
     7681 tgtaaagcgc gacctatcgg cgcagcgcct tcaccgcctg cagcgtctgc ttcacgtgct
     7741 cgcggtaatc catgttcgta tagacgaact tgatccgccg gtccttgccg atgacatagg
     7801 acgtgcgggt cgacaggccg gtggcactgc catccgcctt tttcagcacc acgtcatagg
     7861 cggcgatggt ctggggcgag gcgatggcga cggcgaactt gtcgcggcag gcctcggtcg
     7921 aaaagcgctg gagcgagggc agatcatcgg ccgacatgcc caccaccgtc gccccggcgg
     7981 cgcggaattc ggcgctggcc tcggcaaagg cgcgggcttc gaaggtgcag ccctgggtga
     8041 acgccttggg atagaaatag agcaccaccg gccccttctt cagcgcctgc tccaggttga
     8101 aggtaaagac ctttccggcc aacgcgccct gggcgacgat ctgcggggcc cgcgcgccct
     8161 tgggaagatc ggccggggcc gaagcgggaa gagcaaagac ggaggcagcg gccagcgcgg
     8221 caaagagggt gcgtttcatg cgccaatgat gcggcaagcg gcgcgcgctg tccagcgtgg
     8281 ctattgcccg gccaccccgg gatcagccgc aatggtcggt gcggccttgg gttcggtcgc
     8341 caggcccgga tcgccttcgg ccccggcggg ctgatccttg cgcgtggcgc cgcgccgcag
     8401 cgatttcagc agggcatcgc gctgcgccgc cagagcttcg gtgctgggcc cggtgcgggc
     8461 ttcggattcg gccttgaagg cttcccagat gatcgacgat ttgggatcgc tgcccggcat
     8521 tccgtccagc acccgcttgc cgctgatccg gtccacccgg acccagtgaa tccccttggg
     8581 cgcggtaaag ggccggcggc tccagcgcgg tttggtggcc tggaccatct gcttgaagat
     8641 cggcgcggca atcgtcccgc cctgggcata accgcccagc gacgcgggct gatcgtagcc
     8701 gagatagacc ccgccgacga tttcctggtt gccgccgaca aaccacacat tggtcgggcc
     8761 cgtggtggtt cccgtcttgc cgaacagcgg caggccca
//
LOCUS       DASUDV010000055         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1327229,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000055 DASUDV010000000
VERSION     DASUDV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327229"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..670
                     /locus_tag="VFV30_01165"
     CDS             89..670
                     /locus_tag="VFV30_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX4846727.1"
                     /translation="MAVGGLGFVMVHALPKFNLTFEEYTDFYWDRRYWLYVHLCAGLF
                     ALLSGPLQFVARLRQRHPGVHRWSGRFYLLGTVTAAVTAVWLAWTSPVPQPLYAIGLY
                     LVSLLWLATGWQGWRLIRRRKLDTHREWMIRNYAVTFFFVMFFGIFDGLTAAGWPEEQ
                     WAVAVTLAVWLGILMPLVTAEWVIRSLRQVPNS"
     gene            684..878
                     /locus_tag="VFV30_01170"
     CDS             684..878
                     /locus_tag="VFV30_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846728.1"
                     /translation="MHGAGDPLVPVEAGHDTAAHIPGARLHEIPGMGHDLPLELVDTM
                     ADLIADHAQVASHRVNASTA"
     gene            1099..1602
                     /locus_tag="VFV30_01175"
     CDS             1099..1602
                     /locus_tag="VFV30_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4846729.1"
                     /translation="MKFLIPAAFAACLLVPQSSLADQVDPDVLVRAPTQTQAWMDELS
                     SQLTHGLRYPASPTGQYASGIVAIGFIPGNGNSPAELAVLRSSGSKALDRAALRAVGR
                     LEGLSPLPQGVLEVSVIRANIIFAESKGQLDRYATQLRHDEAARIAASGPEQTQIALT
                     VSNRTPG"
     gene            complement(1700..1960)
                     /locus_tag="VFV30_01180"
     CDS             complement(1700..1960)
                     /locus_tag="VFV30_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX4846730.1"
                     /translation="MLNIIGALISGLFVGALARWFYPGPVEMGWWMTAFLGMGGALVV
                     GVLTSLTSRQGIGEGFNRAGCLASVLGAMALIWIGRHYGWSF"
     gene            2041..3321
                     /locus_tag="VFV30_01185"
     CDS             2041..3321
                     /locus_tag="VFV30_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4846731.1"
                     /translation="MRIMVLGAGGFIGRAIVAELVGHGHQVVTVQRPGRSADALPPGA
                     EALALDLARATSPADWHEAVRGVDMVVNAAGILRGSDMEAVHVAMPVALHQAASEAEV
                     RRVVLISAISARPEVPTDYAQTKLRGEEVLRQSGLGWTILRPSLVYGEGSYGGTSLLR
                     GLAALPWRVPLPGQGQFAFSPIHVRDLARAVREVCEDERHAGQMLDPCGPETLNLRDL
                     LGRYRRWLGFGEARFVPVPMPVMRALGRVGDWLGDGPVSTNSLVQLVAGNAGDGDGFA
                     RTIGFVPRSLGEALRESPAQVQDRWHARLFFLAPAIRWTLAMLWLVSALLGLAWGADQ
                     TRAVVAGLGLPASLADPLRWAGSLLDLVIAAAVLVDRRGRIATGLQFLAVVGYSLVIG
                     LAIPALWLDPLGPLLKNLPILVLILVHGAIAEQR"
BASE COUNT          517 a         1087 c         1099 g          629 t
ORIGIN      
        1 ggaggaagcc gtgtccggac ctacgaccga tctcgcaacc acaagggttc gcgcaacgca
       61 ccgagccatt gccgcgctgt gccttgtgat ggcggtgggc ggcctcggtt tcgtcatggt
      121 tcacgccctg cccaagttta acctgacctt cgaagaatat accgacttct actgggatcg
      181 gcggtattgg ctttatgtcc atctgtgcgc gggcctgttc gcgctgctgt ccggtccgct
      241 gcaattcgtc gcccggctgc gccagcggca tcccggggtg catcgctgga gcggtcgctt
      301 ctatctgctc ggcacggtga ccgcggcggt gacggccgtg tggctggcct ggacaagtcc
      361 cgtcccccaa ccgctatacg ccatcgggct ctacctggtt tcgctgctgt ggcttgcgac
      421 cggctggcaa ggctggcgcc tgatccgccg ccgaaaactt gatacccacc gcgagtggat
      481 gatccgcaac tatgcggtca ccttcttttt cgtgatgttt ttcggcattt tcgacgggct
      541 gacggctgcg ggctggcccg aagagcaatg ggccgttgcg gtgaccttgg ccgtgtggct
      601 gggaatcctg atgccgctgg ttacggcgga atgggtaatc cgttcgcttc gccaggtgcc
      661 gaattcctag cgtcagccag atgatgcacg gcgccggcga tccgctggtt ccggtagagg
      721 ccgggcacga taccgccgcc cacatccccg gcgcgcggct gcacgagatt cccggcatgg
      781 gccacgacct gccgctggaa ctggttgaca ccatggccga cctgatcgcg gaccatgcgc
      841 aagttgcgtc gcacagagtc aatgcaagca ccgcctgaat caaaagttcc gcctcattcc
      901 ggttcgcgcc gcccagtttt tcggactgcc tcaattgcct tgccagcacc cacatcgagg
      961 gcctatggca gcttccgggg agacgcgtgg tccgcagaac gcccgccttc tctttgacct
     1021 tagcacatgc gccgttgggt cgccgctgta agtgcggtgc taggcttgat gcatacccgt
     1081 caaaggaagg actaagtcat gaaattcttg atcccggcgg cattcgccgc ctgcctgctc
     1141 gtgcctcaat catctctggc cgatcaggtt gatccggacg tcctggtgcg cgcaccaacg
     1201 caaacgcagg cgtggatgga tgaactgtca agtcagctta cgcatgggct gcgctatccg
     1261 gcgagcccca ccggccaata cgcgtcaggg atcgtcgcga tcggcttcat ccctggaaac
     1321 ggcaattccc cagcggagct tgcagtgctg cgcagttcgg gaagcaaggc gctcgaccgt
     1381 gcggccttgc gcgcggtcgg ccggttggag ggtctgagtc cgctacctca gggcgtcttg
     1441 gaagtaagcg tcattcgggc caacataatc tttgccgaaa gcaagggcca gcttgatcgc
     1501 tatgccacgc aattgcggca cgacgaagcg gcgcggatcg ccgccagcgg acccgaacaa
     1561 acgcagattg ccctgactgt cagcaatcgc actcccggct gagcttgctc agcggtagtg
     1621 caaggtcttc gcggtcactc ggttcagcgg acgcttgacc gcgaagactg gcttcttgcc
     1681 gaaaattcgc atggaacgat cagaagctcc acccatagtg gcgtccgatc cagatcagcg
     1741 ccatggcgcc cagcaccgag gccaggcagc cggcgcggtt gaaaccttcg ccgatcccct
     1801 ggcgcgaggt caggctggtc agcaccccca ccaccaaggc gccgcccatg cctaggaacg
     1861 cggtcatcca ccagcccatc tcgaccgggc cgggatagaa ccagcgggcc agcgcgccga
     1921 cgaacagccc gctgatcagc gcgccgatga tgttgagcat ggggcatttc tccactgcat
     1981 tggaacaggt gtcgctgcgc ctgattgcca gcacagcccc cgatctggca agcaggcttg
     2041 atgcggatca tggtgcttgg cgcgggcggc tttatcgggc gggcaatcgt tgccgaactg
     2101 gtcggccatg gccaccaggt ggtgacggtg cagcgccccg gccggtcggc cgatgccctg
     2161 ccgccgggcg ccgaagcact ggcgctcgat ctggcgcggg ccacctcgcc cgctgattgg
     2221 cacgaggccg tgcgcggggt cgacatggtg gtcaatgccg ccgggatcct gcgcggctcg
     2281 gatatggagg cggtgcacgt ggcgatgcca gttgcgctgc accaagccgc gagcgaggcc
     2341 gaggtcaggc gcgtggtgct gatttccgcg atcagcgccc gccccgaagt gccgactgac
     2401 tatgcgcaaa caaagctccg cggcgaagag gtgctgcgcc agtccggcct tggctggacg
     2461 atcctgcgcc cctcgctggt ctatggcgaa ggctcctatg gcgggacctc gctgctgcgc
     2521 gggctcgccg cgctgccctg gcgggtgccg ctgcccggac agggccaatt cgctttctcg
     2581 ccgatccatg tccgcgatct cgcccgcgcg gtgcgcgaag tctgcgagga cgagcgccat
     2641 gccggccaga tgctcgaccc ttgcggcccc gaaacgctga acctgcgcga tctgctgggc
     2701 cgctatcggc gctggctggg gtttggcgag gcccgcttcg tgccggtccc gatgcccgtg
     2761 atgcgcgcgc tgggccgggt tggcgactgg ctgggcgatg gcccggtttc aaccaacagc
     2821 ctggtgcaac tggtggccgg caatgcaggc gacggcgacg ggttcgcccg gaccatcggc
     2881 tttgtcccgc gcagtcttgg cgaagcactt cgcgaaagcc cggcgcaggt ccaggaccgc
     2941 tggcacgccc ggctgttctt cctcgccccg gcgatccgct ggacgctggc catgctgtgg
     3001 ctggtatcgg cgctgctggg gctggcctgg ggcgcggatc agacccgggc ggttgtcgca
     3061 ggcctgggac tgccggcgag ccttgccgat ccgctgcgct gggctggctc tctgcttgat
     3121 ctggtgatcg cggcggcggt gctggtcgat cggcgcggcc ggatcgcaac agggctgcaa
     3181 tttctggcag tcgtcggcta cagcctggtg atcggccttg cgatcccggc gctgtggctc
     3241 gatccgcttg gccccttgct caagaacctg ccgatcctgg tattgatcct ggttcacggc
     3301 gcaatcgcgg agcagcgctg atggagccct tc
//
LOCUS       DASUDV010000056         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_24019,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000056 DASUDV010000000
VERSION     DASUDV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24019"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFV30_01190"
     CDS             <1..1176
                     /locus_tag="VFV30_01190"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEX4846732.1"
                     /translation="RTEVESVVNKVEISIRAYAERTSQRKLDNAMQGTAALRHKPHGV
                     MAVLGPYNFPAHLPNGHIVPALIAGNAVVFKPSEKTPATAELLVQCFHRAGISAAVVQ
                     LLIGGPAEGQALVAHEGIDGVLFTGSAHTGIAINRKLATRPDKIVALEMGGNNPMVVW
                     DTPKITDAAALVVQSAFTSAGQRCTAARRLIVKTRMYEPLIAEVKRLADRLIVGAPFD
                     EPAPYMGPVIDNQAADGLTESFLYLLSHGGKAIKHLIRPRDDLPFLSPAIIDVSAVKE
                     KPDVELFGPLLQVSQASDLDEAIAEANNTRFGLSASLIGGTPQEYNRFWANVRAGVIN
                     WNRPTNGASSAAPFGGVGLSGNHRPSAYYAADYCAYPVASTEMEQPRAMIGVGLREG"
     gene            complement(1177..1878)
                     /locus_tag="VFV30_01195"
     CDS             complement(1177..1878)
                     /locus_tag="VFV30_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846733.1"
                     /translation="MDRFLPLLALGLLTACGDAAAPETTPLRDPAIMGALSEPLLSDP
                     DLVGASRNATMLSGGGLAEGGVPLFGPDDKEAARARDEAARLLGGSIAPAPRATGGAE
                     VSAVAGAVTAGGVAATLPFAAKCAASLDYSFAWAARLPAAVPVYPRAHAQEAAGSDAA
                     GCKLRVVNFRTPVAVNDVIDFYHASAARAGLVPQVSKAGKDKVVSGAKDSLSFAVHAR
                     KLADGMTEVDLVTQG"
     gene            complement(1918..2322)
                     /locus_tag="VFV30_01200"
     CDS             complement(1918..2322)
                     /locus_tag="VFV30_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX4846734.1"
                     /translation="MADPDYYRGHDNGWHHGERKRDRWEGRYGDGYRDDYRGDYRDNY
                     RGDYRNSGYYGEPVYRNTRTWRGDDGRYYCRRSDGTTGLIVGGVAGALIGREVAGRRG
                     DRTVGAILGAAGGALLGRAIDKNASNDGYRCR"
BASE COUNT          404 a          800 c          759 g          363 t
ORIGIN      
        1 cgcaccgaag ttgaaagcgt ggtcaacaag gtcgagattt cgatccgcgc ctatgccgag
       61 cggacgagcc agcgcaagct ggacaacgcc atgcagggca ccgccgcgct gcgccacaag
      121 ccgcacggcg tgatggccgt cctgggcccc tacaacttcc cagcccacct gcccaatggc
      181 cacatcgtgc cggcgctgat cgcgggcaac gccgtggtct tcaaaccgag cgagaagacc
      241 ccggcaacgg ccgaattgct ggtgcagtgc ttccaccgtg ctggtatctc ggcagcagta
      301 gtccagctgc tgatcggtgg tcccgccgaa ggccaggcgc tggtcgcgca tgagggcatc
      361 gacggggtgt tgttcaccgg ttcggcccac accgggatcg cgatcaaccg caagctggca
      421 acccgtccgg acaagatcgt cgcgctggaa atgggcggca acaacccgat ggtggtctgg
      481 gacacgccaa agatcaccga tgccgctgcg ctggtggtgc aatcggcctt cacctcggcc
      541 gggcagcgct gcaccgccgc gcgccgactg atcgtcaaga cgagaatgta cgaaccgctg
      601 atcgccgaag tgaagcggct ggccgaccgg ctcatcgtcg gtgcgccctt cgatgaaccg
      661 gcaccctata tgggcccggt gatcgacaac caggccgccg atggcctgac agagagcttc
      721 ctttatctgc tgagccacgg cggcaaggcg atcaagcacc tgatccgccc gcgcgatgac
      781 ctgccgttcc tcagccctgc gatcatcgat gtcagcgcgg tcaaggaaaa gcccgatgtc
      841 gaactgttcg gcccgctgct ccaggtcagc caggcaagcg acctcgacga ggcgattgcc
      901 gaggccaaca acacgcgctt tggcctctcg gcctcgctga tcggcgggac gccccaggaa
      961 tacaaccgct tctgggccaa tgtccgcgcc ggggtgatca actggaaccg gccgaccaac
     1021 ggcgccagct cggccgcgcc gtttggcggg gtcggcctgt ccggcaatca ccgcccaagc
     1081 gcttactacg cggcagacta ttgcgcctat ccggtcgcct cgaccgagat ggagcagccg
     1141 cgtgcgatga tcggggtggg cctgcgcgag ggctgatcag ccctgggtga ctaggtccac
     1201 ttcggtcatg ccatcggcca gcttgcgcgc gtgaaccgcg aaggacaggc tgtccttcgc
     1261 gccgctaacg accttgtcct tgccggcttt tgatacctgc ggcaccagcc cggcccgtgc
     1321 ggcgctggca tggtagaaat cgatcacgtc gttcaccgcc acgggcgtgc ggaaattgac
     1381 cacgcgcagc ttgcacccgg ccgcgtcgct gccggcggct tcctgcgcat gggcgcgcgg
     1441 atagaccggc acggcggcag gcagccgcgc ggcccaagcg aaggaatagt cgagcgaagc
     1501 cgcacatttc gccgcaaagg gcaatgttgc cgcaacgccg ccagccgtga ccgcccctgc
     1561 gacggctgaa acctcggcac cgcctgtggc gcgcggtgcg ggggcaatgg agccgccgag
     1621 cagacgggcg gcttcatcgc gagcgcgggc cgcttccttg tcgtccgggc cgaacagcgg
     1681 caccccgcct tcggccagtc cgccaccaga tagcatcgtg gcgttgcggc tggccccgac
     1741 cagatcggga tcgctgagca gcggttcgga cagcgcaccc atgatcgcgg gatcgcgcaa
     1801 gggggtcgtt tccggcgctg cggcatcgcc gcaggcggtc agtaggccta aagccagcag
     1861 gggcaggaaa cggtccattg cgcgggcagc gatacccgcg cgatggttaa ggtcacttta
     1921 gcggcagcgg tagccgtcgt tgctggcgtt cttgtcgatc gcccggccca gcagcgcgcc
     1981 gccagctgcg cccaggatcg caccaaccgt ccggtcaccc cggcggccag ccacttcgcg
     2041 gccgatcagc gccccagcga caccacccac gatcaggccg gtcgtgccat cgctgcggcg
     2101 gcagtaatag cggccatcat ccccgcgcca ggtgcgggtg ttgcggtaga ccggctcgcc
     2161 ataatagccg ctgttgcggt aatcgccgcg atagttgtcc cggtaatcgc cgcgataatc
     2221 atcacggtag ccatcgccat agcggccttc ccagcggtcg cgcttgcgct caccgtggtg
     2281 ccagccgttg tcgtggcctc gataatagtc agggtcggcc agcgcc
//
LOCUS       DASUDV010000057         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_554149,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000057 DASUDV010000000
VERSION     DASUDV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554149"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2290
                     /locus_tag="VFV30_01205"
     CDS             <1..>2290
                     /locus_tag="VFV30_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4846735.1"
                     /translation="FKYHRPRGILSAGVEEPNALVTVGEGGRTEPNTRASDVFVYEGM
                     VATSQNAWPSLKFDIGAVIGLFSRALPAGFYYKTFFGPAKLWLGYEWFIRRAAGLGKA
                     PTLPDPDRFAQRGAFCDVLVIGGGPAGLAEALKAAEAGEKVILVEQDAVLAPSLRRDP
                     QELTPAWAEASAARIRELGGTVLTRTSAAGYWEHDLVTLTQLLVEPGQVPEQGPAQRL
                     WHVRAGRVVLASGALERPLAFASNDLPGVMLSQAVRTYARRFGVVPGQRVVVATNNDD
                     AYATAHALAELGAEVVAVLDSRPASAARDLAGNLPLFTDARPIAAKGGSGGVRALTAQ
                     IGETQQTFAADLVAMSGGFTPTLHLFMQAGSTLGWDDAAQAFIPGTSRQNITAVGAAA
                     QAAPIHAAEPLGDPKKSFIDFQNDVTLGDVDLAWAEGYRSVEHVKRYTTLGMATDQGK
                     IGNMAALARLARNKGVAVPEAGITTFRPPFTPVTMGLLAGRTHRDAGAHLRRIALYDL
                     HLARNPIWQPLGYWKRPRAYPRGEENLAEATRREARTVRSLVGMTDVSTLAKFEVTGP
                     DAASFLEKICATSVAKLALGRGRYTFMLREDGLVCDDGTVWRLEENRYLLTSSTGGAD
                     RMATVLSYARQVLFPEMRVSVVNVQEHYAGIAVAGPQASAVVAALTGAEAPRHMGLDR
                     ATIAGTPVLVLAASYSGERAFEIYVEGTSAEAVWTACEDEVLGRDGCLYGLEAMEVLR
                     IEKGHVVVGGEVDMRRTPQELGL"
BASE COUNT          369 a          798 c          772 g          351 t
ORIGIN      
        1 ttcaagtacc accgcccgcg cgggatccta tcggccgggg tggaagagcc caacgcgctt
       61 gtcaccgtgg gcgaaggcgg acggaccgaa cccaacaccc gcgccagcga tgtcttcgtc
      121 tacgaaggga tggtcgccac cagccagaac gcctggccca gcctgaagtt cgacatcggc
      181 gcggtgatcg gcctgttttc cagggcgctg ccggcgggct tctattacaa gaccttcttc
      241 ggcccggcca agctgtggct gggctacgaa tggttcatcc gccgcgccgc cggccttggc
      301 aaggccccca ccctgcccga tcccgatcgc ttcgcccagc gcggtgcgtt ctgcgatgtg
      361 ctggtgatcg gcggcggtcc ggcgggcctt gccgaagcgc tcaaggccgc cgaggctggc
      421 gagaaggtga tcctggtcga acaggatgcc gtactcgccc cctcgctccg ccgcgatccg
      481 caggaactca cccccgcctg ggccgaggca agcgccgcgc ggatccgcga gctgggcggc
      541 acggtgctga cccgcaccag cgccgccggt tactgggaac acgatctggt gacgctgacc
      601 cagctgctgg tcgaacccgg acaggttccc gagcaaggcc cggcccagcg cctgtggcac
      661 gtgcgcgcgg gccgcgtggt gctggcaagc ggcgcgctgg agcgtccgct ggcctttgcc
      721 agcaacgatc ttcccggcgt gatgctgagc caggcggtgc gcacctatgc ccgccgtttc
      781 ggtgtggtgc cgggccagcg cgtggtggtc gccaccaaca acgacgatgc ctatgccacc
      841 gcccatgccc ttgccgaact gggagcagaa gtggttgcgg tgctcgatag tcgcccggct
      901 tcggcggcac gcgatctggc cggcaacctg ccgctgttca ccgatgcccg gccaatcgcg
      961 gccaagggcg gcagcggcgg cgtacgcgcg ctgaccgctc agattggcga aacgcagcag
     1021 accttcgccg ccgatctggt ggcaatgtcg ggcgggttca cccccacgct gcacctgttc
     1081 atgcaggccg gatccacgct gggctgggac gatgcggcac aggccttcat ccccggcacc
     1141 tcgcggcaga acatcaccgc cgtaggcgcg gctgcacagg ccgccccgat ccacgccgcc
     1201 gaaccgctcg gcgatcccaa gaaaagcttc atcgacttcc agaacgacgt gacgcttggc
     1261 gatgtcgatc tggcctgggc cgaaggctat cgctcggtcg aacacgtcaa gcgctatacc
     1321 acgctgggca tggccaccga tcagggcaag atcggcaaca tggcagcgct ggcccgcctg
     1381 gcgcgcaaca agggcgtggc cgttcccgaa gccgggatca ccaccttccg cccgccgttc
     1441 accccggtga cgatgggcct gctggcgggg cgcacccacc gcgatgccgg ggcgcacctg
     1501 cggcggatcg cgctttacga tctgcacctg gccaggaacc cgatctggca accgcttggc
     1561 tactggaagc gcccgcgcgc ctatccgcgg ggcgaggaga acctggccga agccacccgc
     1621 cgcgaggcgc gcaccgtgcg cagcctggtg gggatgaccg acgtatcaac cctggccaag
     1681 ttcgaggtga ccggccccga tgccgccagc ttccttgaaa agatctgcgc caccagcgtt
     1741 gccaagcttg ccctggggcg cggccgctac accttcatgc tgcgcgagga cggactggtc
     1801 tgcgacgatg gcaccgtctg gcggctcgag gagaaccgct acctcctgac ctcgtcgacc
     1861 ggcggggccg accggatggc aaccgtgctg tcctatgccc gccaggtgct gttccctgaa
     1921 atgcgggtct cggtggtcaa cgtgcaggaa cattatgccg ggatcgccgt ggctggcccc
     1981 caggccagcg cagtggtggc cgccctgacc ggggccgagg caccgcgcca catggggctc
     2041 gaccgcgcca ctatcgccgg aaccccggtg ctggtgctgg ccgccagcta cagcggcgaa
     2101 cgcgcctttg aaatctatgt cgaaggcacc agcgccgaag ccgtgtggac cgcctgcgaa
     2161 gacgaagtgc tgggccgcga cggctgcctc tatgggcttg aggcgatgga agtgctgcgg
     2221 atcgaaaagg gccacgtcgt ggttggcggc gaagttgata tgcgccgtac cccgcaagag
     2281 cttgggcttg
//
LOCUS       DASUDV010000058         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2122594,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000058 DASUDV010000000
VERSION     DASUDV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122594"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFV30_01210"
     CDS             <1..606
                     /locus_tag="VFV30_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX4846736.1"
                     /translation="GTTKTVVAIFLSPMIGFAIAMVMVLITSWLFKGFQPRRAEGVFK
                     GLHLVSSAAYSISHGGNDAQKTMGIITVLLYSTGQLKGEFHVPEWVVISCYAAIAAGT
                     LSGGWKIIKTMGTKLTKLNHHTGFCASTAGSCVVFGASAMGIPISTTHAITGAVIGTG
                     AARKASAVRWGVAGRVVTAWFITIPASAAVGALFYTLTKIF"
     gene            complement(590..1564)
                     /locus_tag="VFV30_01215"
     CDS             complement(590..1564)
                     /locus_tag="VFV30_01215"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX4846737.1"
                     /translation="MSTPNWFLVDGWARGNPTPVKGMLHGGTLGALRTWSNVLQQAII
                     HLGTAWALWLAWQHGVTWVDMSALVVFYIFTGLGASLGLHRLFSHQSFRVPNWLYFTL
                     AVAASMCMQGSPARWATDHQRHHANSDKAGDPHSPIVDPWGRPMPYWRGLWHAHWGWM
                     FNGVVTDKAVYGRVTWGNPILRFVSNTHYLWIALGLAAAWGWGWWLGGTAEAALTAML
                     WGGPVRGFVMMHGVLSATSLAHRVGWQDYATGDHSRNNRLVVLLTFGDGWHNNHHKFP
                     RSARHGLLPGQIDISAEIIELLERIGLISGVARVPPAMIAREALRKSS"
     gene            complement(1561..>2271)
                     /locus_tag="VFV30_01220"
     CDS             complement(1561..>2271)
                     /locus_tag="VFV30_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846738.1"
                     /translation="MMEDPYIARCVTKPRGYAGDAELIDLIYDRRPPESVSARGLQIF
                     RHGIEFQASEGVRQRRDQAEQFVGEAVRAGKRILCLACGHFREADPLIGQELGAITLV
                     DQDPLSLDVVRGKFGSTVTCHEANVFKYLRGAIARGEKFDLVYTLGLTDYLDDRAMGL
                     LHRMAKAVLAPGGEFILANFMPDHLAIGWMDAIMEWQLIYREPEDLAAFAKAEGLRAE
                     TWRDATGSIAWSRMVLPA"
BASE COUNT          374 a          788 c          754 g          355 t
ORIGIN      
        1 ggcacgacca agaccgtggt ggcgatcttc ctctcgccga tgatcggctt tgccatcgcc
       61 atggtcatgg tcctgatcac ctcgtggctg ttcaaggggt tccagccgcg ccgcgccgaa
      121 ggggtgttca aggggctgca cctggtcagc tcggccgcct attcgatcag ccacggcggc
      181 aacgacgcgc agaagacgat ggggatcatc accgtgctgc tctattccac cgggcagctg
      241 aagggcgaat tccacgtccc cgaatgggtg gtgatttcct gctatgcggc gatcgcggcg
      301 ggcacgcttt cgggcgggtg gaagatcatc aagacgatgg gcaccaagct gaccaagctc
      361 aaccaccaca ccggtttctg cgccagcacg gcgggcagct gcgtggtgtt cggggccagc
      421 gcgatgggca ttccgatttc caccacccat gccatcaccg gcgcggtgat cggcaccggc
      481 gccgcgcgca aggccagcgc ggtgcgctgg ggcgtggccg gccgggtggt gaccgcctgg
      541 ttcatcacca tcccggccag cgccgcggtg ggggcgctgt tctacaccct cacgaagatt
      601 ttctgagcgc ctcgcgcgcg atcattgccg gggggacgcg ggccacgccg ctgatcaggc
      661 cgatccgctc cagcagctcg atgatctcgg cgctgatgtc gatctgtccg ggcagcaggc
      721 cgtggcgggc agagcgcggg aacttgtggt ggttgttgtg ccagccgtcg ccgaaggtca
      781 gcagcacgac cagccggttg ttgcggctgt gatcgccggt ggcatagtcc tgccacccga
      841 cccggtgcgc cagcgacgtg gccgaaagca ccccgtgcat catcacgaag ccgcggaccg
      901 gcccgcccca cagcatcgcg gtcagcgccg cttccgccgt gccgcccagc caccagcccc
      961 agccccaggc cgcggccagc cccagcgcga tccaaaggta atgggtgttg ctgacaaagc
     1021 gcaggatcgg gttgccccag gtcacccggc catagaccgc cttgtcggtg accacgccgt
     1081 tgaacatcca gccccagtgg gcgtgccaca gcccgcgcca atagggcatc ggccggcccc
     1141 acggatcgac gatcggcgaa tgcggatcgc cggccttgtc ggaattggcg tggtggcgct
     1201 ggtggtcggt cgcccagcgc gcgggcgagc cctgcatgca catcgaggcg gccaccgcga
     1261 gcgtgaaata gagccagttc ggcacccgga agctctggtg cgagaacagc cggtgcaggc
     1321 ccagcgaggc gccgaggccc gtgaagatgt agaacaccac cagcgcgctc atgtcgaccc
     1381 aggtcacccc gtgctgccag gccagccaca gcgcccaggc ggtgccgagg tggatgatcg
     1441 cctgctgcag cacgttggac caggtcctga gcgctcccag ggtgccgccg tggagcatgc
     1501 ccttcaccgg ggtcggattg ccgcgcgccc agccatcgac cagaaaccag ttcggcgtgc
     1561 tcatgccggc agcaccatcc gcgaccaggc gatcgatccg gtcgcatcgc gccaggtctc
     1621 ggcccgcagc ccttcggcct tggcaaaggc ggccagatct tccggttcgc gatagatcag
     1681 ctgccattcc atgatcgcgt ccatccagcc gatggccaga tgatcgggca tgaaattggc
     1741 aagaatgaat tcgccgcccg gcgccagcac ggccttggcc atgcggtgca gcaggcccat
     1801 cgcccggtcg tccagatagt cggtcagccc cagggtatag accaggtcga acttctcgcc
     1861 ccgggcgatc gcgccacgca ggtacttgaa cacgttggcc tcgtggcagg tcaccgtgct
     1921 gccgaacttg ccgcgcacca cgtccagcga gagcggatcc tgatcgacca gcgtgatcgc
     1981 gcccagctcc tgcccgatca gcggatcggc ctcgcggaag tggccgcagg ccaggcacag
     2041 gatccgcttg ccggcgcgca ccgcctcgcc cacgaactgc tcggcctggt cgcggcgctg
     2101 gcgcacgccc tcgctggcct ggaactcgat cccgtggcgg aagatctgca ggccccgcgc
     2161 gctgacgctc tccggcgggc ggcggtcata gatcaggtcg atcagctcgg catccccggc
     2221 atagccgcgc ggcttggtga cgcagcgggc gatgtaggga tcctccatca t
//
LOCUS       DASUDV010000059         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_443726,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000059 DASUDV010000000
VERSION     DASUDV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443726"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..883
                     /gene="accD"
                     /locus_tag="VFV30_01225"
     CDS             35..883
                     /gene="accD"
                     /locus_tag="VFV30_01225"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX4846739.1"
                     /translation="MSWLNRVRKALPFKDKRDTPDNLWTSCPSCKEMLFTKEYEENQS
                     VCPRCEHHGRIGADARFAQLLDEGFTILDTPKVKEDPLKFKDQKKYPDRLKAARAANP
                     HPDALTNALGKIGGHKAVLGVQDFAFMGGSMGMAVGAAFVAGVDRAIKEKCAYVICTA
                     AGGARMQEGILSLMQMPKATVAISRLKRAGLPYIVVLTDPTTGGVTASYAMLGDIHIA
                     EPGCLIGFAGQRVIQDTIREKLPEGFQRAEYLHEHGMVDMVVHRRDLKATLATVLDYL
                     GAKAAA"
     gene            1006..2313
                     /locus_tag="VFV30_01230"
     CDS             1006..2313
                     /locus_tag="VFV30_01230"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX4846740.1"
                     /translation="MRDFAISDNPRVQAQLDRLGRLSLPQGRLGLEVIHALLARLGDP
                     HKALPPVFHVAGTNGKGSTCAYLRAMLEAEGLTVHAATKPHLVRYNERIRVAGELVSD
                     DLLADLLQEVLDRGEDLSPSFFEVTTAATFLAFHRIPADACVIEVGLGGRFDATNVLD
                     SQAACGIATLGIDHKEFLLVPEEGVPQEPLCRIAFEKAGIARPGVPLVTQAYAPDVTK
                     TVIDHAMTVGAKTAIRGLDWDAAIGAEIAYRDRLGELTLPLPALAGTHQADNAALAVA
                     MLRHQSQVAVSPEAMADGIRAARWPARLQLLGDGPLTALVPGRKVWLDGGHNADAGLA
                     IGRHFAGSPPLHLITGMLANKDPAAIVAPLKGKLLSLSVVPAPGHEAHRPEDIAVHAD
                     RPVRPFGDVGAALRALPPEGDVLIAGSLYLAGEVLRLNREFPD"
     gene            complement(2314..3657)
                     /locus_tag="VFV30_01235"
     CDS             complement(2314..3657)
                     /locus_tag="VFV30_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4846741.1"
                     /translation="MNESSQSVASERRSLWDSVRPYFEKESLAAFFVGVSSGFPYAMI
                     GATLTTRLAQDGISKKTVTAFTLAFLVYNLKFLWAWVVDGVKLPIIGRLGQRVSWMLV
                     AGLFVMAAVINLALVDPKADIAWTATSAILVGIAGATYDIVIDAYRIETLKPYQLGVG
                     SGMSQYGWRIGSTAAGALALVVAARYDWSVAYMACALLALPAMLTALIVGEPARHRDP
                     VERKGLMAGLHAIAGPFVEFFKRSGAWLVLLFILLHKIGDTLGQLVLRLLLNDMGYTN
                     DEIAIWDVGVGFWAFLIGIFVGGVLYARIGLKKSVLLALILMGVSNASYAVLASVGHT
                     NIGLGLTQGFENFASGIGGVVVVAYFSALCDLRFTAAQYALISAAASVVGRVLTGTSA
                     GAMVESMGYVNYYLMTTVIALPGILLFWWMSRIGLIDAAMGTAGGGKDGNPETPH"
BASE COUNT          644 a         1259 c         1205 g          558 t
ORIGIN      
        1 ttgccgatgc cgtccattcc gctagggaaa tcgcatgagc tggctcaacc gtgttcgcaa
       61 ggcgctgccc ttcaaggaca agcgcgacac ccccgacaac ctttggacaa gctgcccttc
      121 gtgcaaggaa atgctgttca cgaaggagta cgaggaaaac cagtcggtct gcccgcgctg
      181 cgagcatcac ggccggatcg gcgcagacgc gcgctttgcc cagttgctgg acgaaggctt
      241 caccatcctg gacaccccca aggtcaagga agacccgctg aagttcaagg accagaagaa
      301 gtatccggac cggctgaagg ccgcccgcgc ggccaatccg caccctgatg cgctgaccaa
      361 cgcgctgggc aagatcggcg gccacaaggc ggtgctgggc gtgcaggact ttgccttcat
      421 gggcggatcg atgggcatgg ccgtgggcgc ggccttcgtc gccggggtgg accgggcgat
      481 caaggaaaag tgcgcctatg tgatctgcac cgccgccggc ggcgcgcgga tgcaggaagg
      541 catcctcagc ctgatgcaga tgcccaaggc cacggtggcg atcagccgcc tgaagcgcgc
      601 gggcctgccc tatatcgtcg tgctgaccga tccgaccacc gggggcgtga ccgccagcta
      661 tgccatgctg ggcgacattc acattgccga gccgggctgc ctgatcggct ttgccggcca
      721 gcgggtgatc caggacacga tccgcgaaaa gctgcccgaa gggttccagc gcgcggagta
      781 tctgcacgag cacggcatgg tcgacatggt ggtgcaccgc cgcgatctga aggccacact
      841 ggccaccgtg ctggattatc tgggcgcaaa ggcggcggcg tgacggcccc tccgcccctt
      901 cggggcacct ccccatcgca agtcgatggg gagagttacg ccgggtctcc ccatcgactt
      961 gcgatgggga ggtggcagtt ccgcaggaac tgacggaggg gcaaattgcg cgacttcgcg
     1021 atctctgaca atccgcgcgt ccaggcgcag ctggaccggc ttggccggct cagcctcccg
     1081 caggggcggc tggggctgga ggtgatccac gcgctgctgg cgcgcctcgg cgatccgcac
     1141 aaggcgctgc ccccggtctt tcacgtcgcc ggaaccaacg gcaagggcag cacctgtgcc
     1201 tatctgcgcg ccatgcttga ggccgagggg ctaaccgttc acgccgcgac caagccgcac
     1261 ctggtccgct ataacgagcg gatccgggtg gccggagagc tcgtttcgga cgatctgctg
     1321 gccgacctgc tgcaagaggt gctggaccgg ggcgaggacc tctcccccag ctttttcgaa
     1381 gtgaccacgg cggccacgtt cctggctttc caccggatac ccgccgatgc ctgcgtgatc
     1441 gaggttggcc tgggcggccg gttcgatgcc accaacgtgc tggacagcca ggccgcctgc
     1501 gggatcgcca ccctggggat cgaccacaag gaattcctgc tggttccgga agagggcgtt
     1561 ccgcaagagc cgctgtgccg gatcgccttc gagaaggcgg gcattgcccg gcccggcgtg
     1621 cccttggtga cccaggccta tgcccccgac gtgaccaaga ccgtgatcga ccacgcgatg
     1681 accgtcggcg cgaagacagc gatccgcggg ctggactggg acgccgcgat cggtgcggag
     1741 atcgcctacc gcgaccggct gggcgagctg acgctgcccc tccccgcgct ggccggcacg
     1801 caccaggccg acaacgccgc gctggccgtg gcaatgctgc gccaccagag ccaggtcgcg
     1861 gtctctcctg aagccatggc cgatgggatc cgcgccgcgc gctggcccgc gcggctgcag
     1921 ctgctgggtg atggcccgct gaccgcgctg gtgccgggcc gcaaggtctg gctggacggc
     1981 gggcacaacg ccgatgccgg gctggcgatc ggacgccatt tcgctggcag cccgccgctc
     2041 cacctgatta ccgggatgct ggccaacaag gatccggccg ccatcgtcgc gccgctgaag
     2101 ggcaagctgc tcagcctgtc ggtcgtgccg gcacccgggc acgaagccca caggcctgag
     2161 gacatcgccg tccacgccga ccggccggtc cgccccttcg gcgatgtcgg cgccgcactc
     2221 cgcgccctgc cccccgaagg cgacgtgctg atcgccggct cgctttacct cgcgggcgag
     2281 gtgctgcggc tcaaccggga gtttccggac tgatcagtgc ggggtttcgg ggttcccgtc
     2341 cttgccgccg ccggccgtcc ccatggccgc atcgatcagg ccgatccggc tcatccacca
     2401 gaacagcagg atgcccggca gggcgatgac cgtggtcatc aggtaatagt tcacatagcc
     2461 catgctttcg accatggccc cggccgacgt cccggtcagg acccggccga ccacgctcgc
     2521 cgcggcggag atcagggcgt attgcgcggc ggtgaagcgc aggtcgcaca gcgccgagaa
     2581 gtaggcgacc acgaccacgc ctccgatccc gctggcgaag ttctcgaacc cctgggtcag
     2641 gccaaggccg atgttggtgt ggccgacgct ggccagcacg gcgtagctcg cgttcgaaac
     2701 gcccatcagg atgagcgcca gcagcaccga tttcttgagc ccgatccggg cataaagcac
     2761 cccgcccacg aagatcccga tcaggaacgc ccagaaccca accccgacat cccagatcgc
     2821 gatctcgtcg ttggtgtagc ccatgtcgtt gagcagcagg cgcagcacca gctggcccag
     2881 cgtgtcgccg atcttgtgga gcaggatgaa cagcagcacg agccaggccc cgctgcgctt
     2941 gaagaattcg acgaaaggcc cggcgatcgc gtggagcccg gccatcagcc ccttgcgttc
     3001 caccgggtcg cggtggcggg cgggttcgcc cacgatcagc gcggtcagca tcgcgggcag
     3061 cgccagcagc gcgcaggcca tataggccac gctccagtca taccgcgcag ccaccactag
     3121 cgcgagcgcg cccgcggcgg tcgatccgat ccgccagccg tattgcgaca tgccggaccc
     3181 gaccccgagt tggtaaggct tcagcgtctc gatccggtag gcgtcgataa cgatgtcata
     3241 ggtcgccccg gcaatcccga ccaggatggc cgatgtcgcg gtccaggcaa tgtccgcctt
     3301 gggatcgacc agggccaggt tgatcacggc cgccatcacg aacaggccgg ccaccagcat
     3361 ccacgaaacc cgctgcccaa gccgcccgat gatcggcagc ttcaccccgt cgaccaccca
     3421 ggcccacagg aacttgagat tgtagaccag gaaggccagc gtgaaggcgg tgacggtttt
     3481 cttgctgatc ccgtcctgcg ccagccgcgt ggtcagcgtc gcgccgatca tcgcataggg
     3541 aaagcccgac gaaacgccca cgaagaacgc ggccagcgat tccttctcga aataggggcg
     3601 gaccgaatcc cacaagctgc ggcgttcaga cgctacggat tgactggatt cgttcacggt
     3661 gcttgc
//
LOCUS       DASUDV010000060         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_885717,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000060 DASUDV010000000
VERSION     DASUDV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885717"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VFV30_01240"
     CDS             <1..99
                     /locus_tag="VFV30_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4846742.1"
                     /translation="AAARELGISERTLRYRLAAFREAGLAVAGGRR"
     gene            96..464
                     /gene="fliE"
                     /locus_tag="VFV30_01245"
     CDS             96..464
                     /gene="fliE"
                     /locus_tag="VFV30_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX4846743.1"
                     /translation="MNGPGAIGSAGSIQQIMAMRQQILDRSQLLQQVHTAKAQDGSPV
                     QPQGPAGDFTNALQSALDSVTATQRNAESLTTAYERGEVTDIAKVMLARQEAGIAFEA
                     TLQVRNRLLSAYQDIMRMGV"
     gene            468..2075
                     /gene="fliF"
                     /locus_tag="VFV30_01250"
     CDS             468..2075
                     /gene="fliF"
                     /locus_tag="VFV30_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX4846744.1"
                     /translation="MAELITTNDAAALAGAPASPLARMRSMAGDGAMRRALPWFLGAS
                     ALGGIALTWALLAPAPQRVLYSELSDAERAGVVASLDKAGIAYAIDNQTGTLSVGEDD
                     LYRARALVAQDGGLAVPDAATSLDSLPLGASRTLEGERLRAVREHELTLTIQEIDGVE
                     SVRVHLAEAAKSVFVRDNAPPSASVMVRMARGRQLGDSQVAAVVNLVAGSVPGMSPDA
                     VRVVDQHGRLLSAKGSAENDRLELQGRMEDKLRAQIDQLLTPMLGANMFTSEIQIELD
                     LDQVTSARESYDKDGVVRSESQAQSQSTGGAQAGGVPGVLSNTPPPASAVRVGAPQPG
                     MVPGAPAGAGSGESSATRTYELGREVSVANAAPGRVKRLSVAVAISQAALKKAKPEEI
                     EQIKALVTAAVGADPQRGDQVQVLVRPFEPVSDEAPPFYEAPWFAMVVRSAVALIGVL
                     LVLLLAVKPLIGVLRRRGENAANGDSAPGAEDFPAQAIETGAAGTDPAMLGHQVSLAQ
                     RIVEEKPEDALVALRQMLAQSRPETAA"
     gene            2072..>2236
                     /locus_tag="VFV30_01255"
     CDS             2072..>2236
                     /locus_tag="VFV30_01255"
                     /inference="COORDINATES: protein motif:HMM:NF026192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX4846745.1"
                     /translation="MSEELQQVAGLGDAERAAVMIMLLEEEQAAAILGELGPEELQLL
                     GEKMCALGDIG"
BASE COUNT          383 a          724 c          791 g          338 t
ORIGIN      
        1 gccgccgcgc gcgagcttgg catatcggag cgcaccctgc gctatcgcct cgccgccttc
       61 cgcgaggccg gcctcgccgt tgccggaggc cggcgatgaa cggcccgggc gcgatcggca
      121 gtgccggttc gatccagcag atcatggcga tgcgccagca gatcctcgat cgttcgcaac
      181 tgctgcaaca ggttcatacg gccaaggcgc aagacggttc cccggtccag ccccagggcc
      241 cggcgggcga tttcaccaat gcgctgcaat ccgcgctcga cagcgtcacc gcaacccagc
      301 gcaacgccga aagcctgacc accgcctacg aacgcggcga ggtgaccgat atcgccaagg
      361 tcatgctggc gcgccaggaa gcaggcattg cctttgaagc aacgctgcaa gtcaggaacc
      421 ggctgctctc cgcctaccag gacatcatgc ggatgggagt gtgatgaatg gctgagctga
      481 tcaccaccaa cgatgccgcc gcgctggctg gcgcacctgc cagcccgctg gcgcgaatgc
      541 ggagcatggc cggcgatggc gcgatgcgcc gggccctccc ctggtttctt ggcgcgagcg
      601 cgctgggcgg cattgcgctg acctgggcgc tgcttgcccc cgccccgcag cgcgtgctct
      661 attccgagct cagcgatgcc gaacgcgccg gagtggttgc ctcgctcgac aaggcgggga
      721 tcgcctatgc gatcgacaac cagaccggca ccctgagcgt gggtgaagac gatctctatc
      781 gcgcccgggc gctggttgcc caggatggcg gcctggcggt tcccgacgcg gcaacttcgc
      841 tcgattcgct gccgctgggt gccagccgga cgcttgaggg cgagcggctt cgcgcggtgc
      901 gcgaacacga actgacactg acgatccagg agatcgatgg ggtcgaaagc gtgcgcgttc
      961 acctcgccga ggcggccaag tcggtcttcg tgcgcgacaa tgccccgcct tccgcctcgg
     1021 tcatggtccg catggcccgt ggccggcagc tcggcgacag ccaggttgcc gcagtggtca
     1081 acctggttgc cggatcggtc ccggggatga gcccggatgc ggtgcgtgtg gtcgatcagc
     1141 acgggcgcct gctttcggcc aagggcagcg ccgaaaacga ccggttggaa ctgcaaggcc
     1201 ggatggaaga caagctgcgc gcgcagatcg accagttgct gaccccgatg ctgggcgcga
     1261 acatgttcac cagcgagatc cagatcgaac tcgatctcga tcaggttacc tcggcccgcg
     1321 aaagctacga caaggacggc gtggtccgaa gcgaaagcca ggcccagtcg cagagcacag
     1381 gcggagcgca ggccggcggg gtgcccgggg tgctttcgaa tactccgcca cccgccagcg
     1441 cggtgcgcgt cggcgccccg cagcccggca tggtgcccgg cgctccggcc ggtgcgggaa
     1501 gcggcgaatc gagcgcaacg cgcacctacg agcttggccg cgaagtttcg gtcgccaatg
     1561 ccgcgccggg ccgggtcaag cggctctcgg tggcggtcgc gatcagccag gcggcgctca
     1621 aaaaggccaa gcccgaagag atcgagcaga tcaaggctct ggtcaccgcg gccgttggcg
     1681 ccgatccgca gcgcggcgat caggttcagg tcctggtccg tccgttcgaa ccggtgagcg
     1741 acgaggcccc gccattctat gaggcaccct ggtttgccat ggtggtgcgc agcgcggtgg
     1801 cgctgatcgg cgtgctgctg gttctgctgc tggcggtaaa gccgctgatc ggcgtgctgc
     1861 gacggcgcgg cgagaatgcg gcgaacggtg atagcgcacc cggtgccgag gatttcccgg
     1921 cacaggctat cgaaacgggt gcggcgggaa cggatccagc catgctgggc caccaggtca
     1981 gcttggcgca acggatcgtc gaagagaagc ctgaagacgc gctggtggcg ctgcgccaga
     2041 tgcttgccca gtccaggccg gagaccgcgg catgagcgag gaactgcagc aagtcgccgg
     2101 ccttggcgat gccgagcgcg cggcggtgat gatcatgttg ctggaggaag agcaggcagc
     2161 ggcaatcctt ggcgaactgg ggcccgaaga gctccagctg ctgggcgaaa agatgtgcgc
     2221 gctgggtgat attggc
//
LOCUS       DASUDV010000061         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_664680,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000061 DASUDV010000000
VERSION     DASUDV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664680"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFV30_01260"
     CDS             <1..260
                     /locus_tag="VFV30_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX4846746.1"
                     /translation="LAEPGWSILPSAPREAFTGGAHGSDPAVPDMAALFIGHGPSFAP
                     GIRLVPFDNVAITPLLRHLLGLPPAAHLDGSTAPFTRVLRP"
     gene            275..724
                     /locus_tag="VFV30_01265"
     CDS             275..724
                     /locus_tag="VFV30_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4846747.1"
                     /translation="MLYFEDLEVGATASFGRYEVTREEVIDFARKYDPQPFHLSDEAA
                     AQTHFGRISASGWHTCAMVMRMLVDNLTERKQAGLGSPGQDELRWHRPVYPGDVLRVE
                     TELLEKKRSRSRPEMGSLRSAIRVYNQDDQLVMSMIGIGLIRVRDAA"
     gene            721..1146
                     /locus_tag="VFV30_01270"
     CDS             721..1146
                     /locus_tag="VFV30_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846748.1"
                     /translation="MNEADLTPEERAEQAATQANRDAMRAEIRTHEAEYSSLPIVIVC
                     AIPLLVFGSLLLSMLGLESKWWYLALVGIPALIGYFYPLILIDRLKTPLRLEGGKLAT
                     RWQRRKWLIGLAAALTLWLFRFEIYATLETLWNNWQSGW"
     gene            complement(1192..1521)
                     /locus_tag="VFV30_01275"
     CDS             complement(1192..1521)
                     /locus_tag="VFV30_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846749.1"
                     /translation="MRSEPLVYYRQRWPDAEVPADAPVWLHFEVSPSADAVLRTVEVF
                     GNGSITRNSLAIEQRNGDHCPSLIDCSLAEGFEGMALEPIVAAAFEALWEQGVDAPFW
                     FPNDPPR"
     gene            complement(1522..3315)
                     /gene="mutL"
                     /locus_tag="VFV30_01280"
     CDS             complement(1522..3315)
                     /gene="mutL"
                     /locus_tag="VFV30_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX4846750.1"
                     /translation="MPTIRRLPEHLVNRIAAGEVVERPASALKELVENAIDAGARSIA
                     VRLSEGGLGGIEVTDDGCGMSPDEMALALERHATSKLPDEHIEQVVTLGFRGEALPSI
                     ASVARLTLESRPRSAAEGWRRVIDHGAVVEEGPAALPPGTRIRMEDLFGKVPARRKFL
                     RSARSEYAACLDVVRRLAMARPDIGFVLEHDGRRVLAVQPGEELANRVAQIVARELAE
                     DGVAIDLERGAARLTGVAGLPTFNRGVADHQYLFVNGRPVKDRLLVGAVRGAYADMLA
                     RDRHAVLALFLDVPPEDVDVNVHPAKTEVRFRDPAFVRGFIVSGLRHALDSEGRRSAQ
                     APAAPAMAAWQAEPIARAPEPAAALGSLFARDHAAPVVRDAAPAWRPPGEAGLALPAA
                     RAEEAVLAPSVEHPLGVARGQVAQTYIVAEAEDGLVIVDQHAAHERLVLERLKAAGAQ
                     GTVAASQALLIPEVVELDEPDCDRVESAAPALAEFGLALERFGPTAILVRAIPAVLGK
                     GNVAALVRDIADDLAQNGAALLLGERLDHVLATMACHGSVRAGRVLSVAEMNALLREM
                     EVTPRSGQCNHGRPTWVKLAHGDIEKLFGRK"
     gene            3501..>4348
                     /locus_tag="VFV30_01285"
     CDS             3501..>4348
                     /locus_tag="VFV30_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4846751.1"
                     /translation="MASFLSRFLKFGSQNMAIDLGTANTLVYVQDRGIVLNEPSVVAI
                     ETINGVKRVKAVGDDAKMMMGKTPDSIEAIRPLRDGVIADIEIAEEMIKHFIRKVHGK
                     KSMFRYPEIVICVPSGSTSVERRAIRDAASNAGASAVYLILEPMAAAIGADMPVTEPV
                     GSMVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAIVSYVRRHHNLLIGESTAER
                     IKKDYGCATVPADGVGETIHLKGRDLVNGVPKEITITQANIAEALSEPIGAIVEGVRI
                     ALENTAP"
BASE COUNT          714 a         1514 c         1410 g          710 t
ORIGIN      
        1 gcctggccga gccgggctgg tcgatcctgc ccagcgcccc gcgcgaggcc tttactggcg
       61 gggctcacgg ctctgatccg gccgtgcccg acatggccgc gctgttcatc ggccacggcc
      121 cttccttcgc ccccggcatc cggctcgtgc cgtttgacaa cgtggcgatc accccgctcc
      181 tgcgccatct tcttggcctt cctccggcgg cccatctcga tggttccacc gcccctttca
      241 cgcgagtgct ccgcccatga ctgccagcga cgacctgctt tatttcgagg acctggaagt
      301 gggcgcgacc gcatcgttcg ggcgctatga agtgacccgc gaggaagtga tcgactttgc
      361 ccgcaagtac gatccgcagc ccttccacct ctccgacgag gccgccgcgc agacccattt
      421 cgggcggatt tcggccagcg gctggcacac ctgcgcgatg gtcatgcgga tgctggtcga
      481 caacctgacc gagcgcaagc aggccgggct cggctcgccc gggcaggacg agctgcgctg
      541 gcaccgcccg gtctatcccg gcgacgtgct gcgggtggaa accgaactgc tcgagaagaa
      601 gcgcagccga tcgcgcccgg aaatgggcag cctgcgctcg gcgatccggg tctacaacca
      661 ggacgaccaa ttggtgatga gcatgatcgg catcggcctg atccgggtcc gcgacgccgc
      721 gtgaacgagg ccgacctcac cccggaagag cgggccgaac aggccgccac ccaggccaac
      781 cgcgacgcga tgcgggccga aatccgcacc cacgaggcgg aatacagctc gctgccaatc
      841 gtgatcgtct gcgcgattcc gctgctggtc ttcggctcgc tgctgctctc aatgctcggg
      901 ctggaatcga agtggtggta tctggccctg gtcggcattc cggcgctgat cggctatttc
      961 taccctctga ttctgatcga ccgactgaag accccgctgc gactggaggg cggcaagctg
     1021 gccacacgct ggcagcgccg caaatggctg atcggcctgg cggcggcact gacgctgtgg
     1081 ctgttccgct ttgagatcta cgccacgctc gagacgctat ggaacaattg gcaatccgga
     1141 tggtgaggct gcgggatccc aatcgataac tgccgctttg ggcggttcac tttagcgagg
     1201 tgggtcgttg ggaaaccaga acggagcgtc aacgccctgc tcccacagcg cttcaaatgc
     1261 cgcggcaacg atcggctcca aagccattcc ctcaaatcct tctgctaatg agcagtcgat
     1321 caatgaagga caatggtctc cgttccgctg ctcgatcgct aagctgttgc gggtgatgct
     1381 gccattgcca aaaacttcaa ccgttctgag aaccgcgtca gcgctggggc tcacttcgaa
     1441 atgcagccaa accggggcgt cggctgggac ctccgcatcg ggccaacgtt ggcgatagta
     1501 gaccaaaggt tcggagcgca cttacttcct cccgaacagc ttctcgatat cgccgtgggc
     1561 cagtttcacc caggtcggac ggccgtggtt gcactggccg gagcgcgggg tgacttccat
     1621 ttcgcgcagc agggcgttca tttccgcgac cgagagcacc cgcccggcgc ggaccgagcc
     1681 gtggcaggcc atggtcgcca gcacatggtc gagccgttcg ccgagcagca gcgcggcgcc
     1741 gttctgggcc aggtcgtcgg caatgtcgcg gaccagcgcc gccacgttgc ccttgcccag
     1801 cacggccggg atcgcacgga ccaggatcgc ggtcggacca aaccgttcga gcgcgaggcc
     1861 gaattccgcc agcgccgggg cggcactctc caccctgtcg cagtcgggct cgtccagctc
     1921 gaccacttca gggatcagca gcgcctggct ggcggcgacc gtgccctgag ccccggcggc
     1981 cttcagccgt tcgagcacca ggcgttcgtg cgctgcgtgc tggtccacga tcaccagccc
     2041 gtcctcggcc tcggcgacga tataggtctg cgccacctgg ccgcgcgcca cgccgagcgg
     2101 gtgctcgacc gagggagcca ggacggcctc ttccgctcgc gcggccggca gggccaggcc
     2161 ggcctcgcca ggcgggcgcc aggccggggc agcatcgcgg acgacgggcg cggcgtggtc
     2221 tcgggcgaac agcgaaccca gggcggcggc gggttcgggc gcgcgggcga tcggctcggc
     2281 ttgccaagcg gccatggcgg gcgcggccgg agcctgcgcg ctgcgccgcc cctcgctgtc
     2341 gagcgcgtgg cgcaggccgg agacgatgaa accgcggacg aaggccggat cgcggaagcg
     2401 cacttcggtc ttggccgggt ggacgttgac gtcgacatcc tcgggcggaa cgtcgaggaa
     2461 cagggccagc accgcgtggc ggtcgcgcgc cagcatgtcg gcgtaggccc cccgcaccgc
     2521 gccgaccagc agccggtcct tcaccgggcg gccgttgacg aacaggtact ggtgatcggc
     2581 caccccgcgg ttgaatgtcg gcaggccggc caccccggtc agccgggccg ctccgcgttc
     2641 cagatcgatc gccaccccgt cctcggccag ttcgcgcgcg acgatctggg ccacccggtt
     2701 cgccagttcc tcgcccggct gcaccgccag cacccgccgc ccgtcgtgtt ccagcacaaa
     2761 gccgatgtcc ggccgggcca tggccaggcg ccgcaccacg tccaggcagg cggcgtattc
     2821 gctgcgggcc gagcgcagga acttgcgccg ggccggaacc ttgccgaaca gatcctccat
     2881 ccggatccgg gtgccgggcg gcagcgcggc cggcccttcc tccaccacgg ccccgtgatc
     2941 gatcacccgc cgccaccctt cggctgcgct gcgcgggcgg ctctccagcg tcagccgggc
     3001 gacgctggcg atcgagggca gcgcctcgcc gcggaagccc agggtgacga cctgttcgat
     3061 atgctcgtcc ggcaatttcg aggtggcgtg ccgttccagc gccagggcca tctcgtccgg
     3121 gctcatcccg cagccgtcgt cggtcacctc gatcccgcca aggccgcctt cgctcagccg
     3181 gaccgcgatg ctccgcgccc cggcgtcgat ggcgttttcg accaattcct tcagcgccga
     3241 agccggccgt tcgaccacct cgcccgcggc gatgcggttg accaggtgtt cgggaaggcg
     3301 gcggattgtc ggcatcgatt cctgcctagc cgccatgccc cggaaattcg agacggaatg
     3361 ccccgcaaat ccggtcaatc gacacaattt tttggccttt cctcgtgccc cctgctaggg
     3421 ggtccacttc cacgccaacg caacggcctt tcgctgccgc gcgagtcgcg gtatgtggct
     3481 gaaaacttac ggatttttca atggcttcgt tcctgtctcg attcttgaaa ttcggctccc
     3541 agaacatggc gatcgacctc ggtacggcca acaccctggt ctatgtccag gatcgcggga
     3601 tcgtgctgaa cgaaccctcg gtcgtggcga tcgaaacgat caacggcgtg aagcgggtca
     3661 aggccgttgg cgacgatgcc aagatgatga tgggcaagac cccggacagc atcgaagcga
     3721 tccgcccgct gcgtgacggc gtgatcgccg acatcgaaat cgccgaagaa atgatcaagc
     3781 acttcatccg caaggtccac ggcaagaagt cgatgttccg ctatccggaa atcgtgatct
     3841 gcgtcccctc gggatcgacc tcggtggaac gccgcgcgat ccgcgatgcg gccagcaacg
     3901 ccggtgccag cgcggtctac ctgatcctgg agccgatggc cgccgcgatc ggcgccgaca
     3961 tgccggtgac cgaaccggtc ggctcgatgg tggtcgacat cggcggcggc acgaccgaag
     4021 tcgcggtgct ctcgctgcgc ggtctggcct acaccacttc ggtccgcgtc ggcggggaca
     4081 agatggacga ggcgatcgtt tcctacgtgc gccgccacca caacctgctg atcggtgaat
     4141 ccaccgctga gcggatcaag aaggattacg gctgcgccac cgtgccggcc gacggcgtgg
     4201 gcgaaacgat ccacctcaag ggccgcgacc tggtcaacgg cgtgcccaag gaaatcacca
     4261 tcacccaggc gaacatcgcc gaagcgctgt ccgaaccgat cggcgcgatt gttgaaggcg
     4321 tgcgcatcgc gctggaaaac accgcgcc
//
LOCUS       DASUDV010000062         4634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1658748,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000062 DASUDV010000000
VERSION     DASUDV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658748"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFV30_01290"
     CDS             complement(<1..854)
                     /locus_tag="VFV30_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4846752.1"
                     /translation="MRIEPTLRALADPTRLRIMRLLSTMELAVGELALVLGQSQPRVS
                     RHIRILCDAGLAERRKEGSWVFLRSAIGEHRAPPLGAAAARLLATAEEGDAQFAARCA
                     EDRRHLAAIRAARASAAQAYFARHATEWDELRGLHSPDGPVEAALLAALAPDGLGALL
                     DVGTGTGRIAELLSPRAARVTGLDKSPEMLRLARARLQDLPADRLDLVQGDFTALPFA
                     DAAFDTVVFHQVLHYAQEPEHALAEAARVCREGGTIAIVDFAAHDREELRSQHAHARL
                     GFSDEQMLA"
     gene            1020..1241
                     /locus_tag="VFV30_01295"
     CDS             1020..1241
                     /locus_tag="VFV30_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846753.1"
                     /translation="MKKLIAAAFASTAALALAACGSSESAKEEAQADNVEMPAEEGIP
                     EGVGTPVADASAGADAAAASTPASEAPKP"
     gene            1333..1542
                     /locus_tag="VFV30_01300"
     CDS             1333..1542
                     /locus_tag="VFV30_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846754.1"
                     /translation="MRKILLPALVLLLAACGDDNRTAGDVTKSEADALDDAAEMVEQQ
                     RLPAEAAQPPAPSAAPSGESAKPGG"
     gene            1611..2519
                     /locus_tag="VFV30_01305"
     CDS             1611..2519
                     /locus_tag="VFV30_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4846755.1"
                     /translation="MLEGKVVAVTGAGRGVGREIALLCAAHGAAVVVNDPGVGGGGEG
                     GDAGPAQQTVDDIVAAGGKAHANLASVADPAGATSIVEDAVQRFGRIDAVVNNAGILR
                     DAIWHKMSFEDWRAVIDVHLHGAFLVSKAATPYFRGQQSGSFIHFTSTSGLIGNIGQA
                     NYSAAKLGIVGLSQSIALDMARAGVRSNCIAPFAWSRMTASIPAETPEQKERVERLKT
                     MSADKIAPLVVYLASDASQGVTNQIFSVRKNEIVLYNKPRPVRSMTKVEGWTPEAIAD
                     ELIPAFKPSMARGDEVSAHVFPYDPI"
     gene            2530..3708
                     /locus_tag="VFV30_01310"
     CDS             2530..3708
                     /locus_tag="VFV30_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4846756.1"
                     /translation="MSNPGMDPEVFEQFIDQLRRYVRERLIPAEEEVIAADAIPADIL
                     QEMREMGLFGITIPEEFGGAGMNISQYIETVKQLSYASPAYRSTISINIGMTGSAIKN
                     FGTPEQKAEWLPRIASGEIACFGLTEPGSGSDSAAMQTSAVRDGNGYKLNGTKRYITN
                     SPHAKIGLIMARTSKEALPKNAHVSCFIVDMTTPGITIGKPDKKMGQSGAHIADVIME
                     DVHIPGSALVGGEEGKGFRIAMQSLDNGRLSVAGMSVGMGRRALDTAIRYATERKAFG
                     EPIANFQLIQAMLADSEAELYAAECMIADACARADRGENIVRKAAAAKLFASETCGRV
                     VDRCVQIYGGAGYLAEYPAERFFRDARILRIYEGTSQIMQLQIAKHLLREFASEGAVW
                     "
     gene            3715..>4634
                     /locus_tag="VFV30_01315"
     CDS             3715..>4634
                     /locus_tag="VFV30_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4846757.1"
                     /translation="MYDILSGLKIVEVSSFVASPTAGLYCAQFGAEVVRIDHKAGGLD
                     YDRYMMTREGRSLSWENLNRAKKSVALDLQSAEGRELAAELVRATGQMITNLPVNSFL
                     AHDALAEGRPDLVSVRIMGWHDGRQAMDFTVNAASGYPLMCGPEDWDPATAPPVNQIL
                     PAWDFITGAYCAFALLAGLRHRDATGQGIEMRVPLGDVAIGTMANAGAMAEMLYRGAD
                     RERIGNAIWGAFGRDFLSRDGKRFMVAALTPKQWKATVEAFGLSAEVAALEAELGVSF
                     LASDHFRFVHRHRLFDLFQAAADRFDYADLA"
BASE COUNT          790 a         1559 c         1589 g          696 t
ORIGIN      
        1 gccagcatct gctcgtcgga aaagcccagg cgggcgtggg cgtgctggct gcgcagctcc
       61 tcgcggtcgt gcgcggcaaa atcgacgatc gcgatcgtgc ccccttcgcg gcagacccgc
      121 gcggcttcgg caagggcgtg ctcgggctcc tgcgcgtagt gcagcacctg gtggaacacc
      181 acggtgtcga acgccgcatc ggcgaagggc agggcggtga aatcgccctg gaccagatcc
      241 agccggtcgg ccggcaggtc ctgcagccgg gcgcgggcca ggcgcagcat ctcggggctc
      301 ttgtcgagcc cggtcacccg cgcggcgcgg gggctgagca gttcggcgat ccgcccggtg
      361 ccggtgccga cgtcgagcag cgcgcccagc ccgtccggcg ccagggcggc gagcagcgcg
      421 gcttcgaccg gcccgtcggg cgaatggagg ccgcgcagct cgtcccattc ggtggcatgg
      481 cgggcaaaat aggcctgggc ggcgctggcg cgggcggcac ggatcgcggc caggtggcgg
      541 cggtcttcgg cgcagcgggc ggcgaactgg gcgtcgccct cctcggcagt ggccagcagg
      601 cgggccgcgg cggcgccgag cggcggggcg cggtgttcgc cgatcgcgct gcgcaggaac
      661 acccaggagc cttccttgcg ccgttcggcc aggcccgcat cgcacaggat ccggatatgg
      721 cgcgagacgc gcggctggct ttgccccagc accagcgcca attcgcccac ggccagctcc
      781 atcgtgctga gcaggcgcat gatccgcagc cgcgtcgggt cggccagggc gcgaagggtg
      841 ggttcgatcc gcatcggcac atcatataaa gaaatcttta tatccgatca accgctcacc
      901 gtggaaatgt ttcggagggc ggcttttggc ggttggcagg gccgcagcgc agggttacac
      961 aggcggcaat ccggtgcaat tgcgccgttt gacctccttt tgcgacgaga gggtacccga
     1021 tgaagaaact gattgccgcc gcctttgctt ccaccgctgc cctggccctg gcggcctgcg
     1081 gttcgtcgga aagcgccaag gaagaggcgc aggccgacaa cgtggaaatg ccggccgagg
     1141 aaggcattcc cgaaggcgtg ggcacgccgg tggccgatgc ttcggccggt gccgatgccg
     1201 ccgcggccag caccccggcc agcgaagcgc ccaagcccta agcgccgctt gcgacaggat
     1261 cgggcgggga gcacggcagc gggccgcgct ccccgccctt tttctttgcg ccgatccggc
     1321 tatggtcggt ccatgcgcaa gattctgttg cccgccctgg tcctgctgct cgccgcctgc
     1381 ggggacgaca accgcaccgc cggcgacgtc accaaaagcg aagccgacgc gctcgacgat
     1441 gcggccgaaa tggtcgagca gcagcggctt ccggccgagg ccgcccagcc gccggcaccg
     1501 tccgccgcgc cctctggcga aagcgccaaa cccggcggtt gattttgccg cgcggcttcg
     1561 taagggtctt aagcgatcga ttaaaccgga ggattcgcga agccatgggc atgcttgaag
     1621 gcaaggtggt tgccgtaacc ggtgcgggac gcggcgtggg gcgcgagatt gccctgctct
     1681 gcgcagcgca cggagcggcc gtggtggtca acgatccggg cgtgggcggc ggcggcgagg
     1741 gcggcgatgc cggcccggcg cagcagaccg tggacgacat cgttgccgca ggcggcaagg
     1801 cccacgcgaa cctcgccagc gtggccgatc cggccggagc gaccagcatt gtcgaagacg
     1861 cggtccagcg ctttggccgg atcgatgcgg tggtcaacaa cgccgggatc ctgcgggacg
     1921 cgatctggca caagatgagc ttcgaggact ggcgcgcggt gatcgacgtg caccttcacg
     1981 gcgccttcct ggtgtccaag gccgccactc cctatttccg cgggcagcag agcggcagct
     2041 tcatccactt cacctctacc tcgggcctga tcggcaatat cggccaggcg aattactcgg
     2101 cggccaagct cggcatcgtc ggcctgtcgc agtcgatcgc gctcgacatg gcccgcgcgg
     2161 gcgtgcgctc caactgcatc gcgcctttcg cctggagccg gatgaccgcc tcgatcccgg
     2221 ccgaaacgcc cgagcagaag gagcgggtcg aacggctcaa gacgatgagc gccgacaaga
     2281 tcgccccgct ggtggtctat ctcgcctccg atgccagcca gggggtgacc aaccagatct
     2341 tctcggtccg caagaacgag atcgtgctct acaacaagcc gcgtccggtc cgttcgatga
     2401 ccaaggtcga gggctggacg cccgaggcca ttgccgacga gctgatcccc gccttcaagc
     2461 ccagcatggc ccgcggcgac gaggtttcgg cccacgtctt cccctatgac ccgatctgag
     2521 gaccccgcga tgagcaaccc cggcatggac cccgaagtct tcgaacagtt catcgaccag
     2581 ctgcgccgct atgtgcgcga gcggctgatc ccggccgagg aagaggtgat cgcggccgat
     2641 gcgattcccg ccgacatcct gcaggaaatg cgggaaatgg gccttttcgg gatcaccatt
     2701 cccgaagagt tcggcggcgc cgggatgaac atcagccagt acatcgagac ggtgaagcag
     2761 ctgagctacg cctcgcccgc ctaccgctcg accatatcga tcaacatcgg gatgacgggc
     2821 tcagcgatca agaacttcgg cacgcccgaa cagaaggccg aatggctgcc gcggatcgcc
     2881 agcggcgaga ttgcctgctt cggcctgacc gagccgggct cgggatcgga cagcgcggcg
     2941 atgcagacct cggccgtccg cgatggcaac ggctacaagc tcaacggtac caagcgctac
     3001 atcaccaaca gcccgcacgc gaagatcggg ctgatcatgg cccgcaccag caaggaagcg
     3061 ctgcccaaga acgcgcacgt gtcctgcttc atcgtcgaca tgaccacgcc ggggatcacc
     3121 atcggcaaac ccgacaagaa gatgggccag tccggcgcgc acattgccga cgtgatcatg
     3181 gaggacgttc acattccggg ttcggcgctg gtcggcggcg aggagggcaa gggctttcgc
     3241 atcgcgatgc agagcctcga caacggccgc ctctcggtcg ccggcatgag cgtcggcatg
     3301 ggccgccgcg cgctcgacac cgctatccgc tatgcgaccg agcgcaaggc ctttggcgaa
     3361 ccgatcgcca acttccagct gatccaggcc atgctggccg acagcgaggc cgaactctac
     3421 gccgccgaat gcatgatcgc ggatgcctgc gcccgcgccg accggggcga gaacatcgtc
     3481 cgcaaggccg ccgcggcgaa gctgttcgct tcggaaacct gcggccgggt ggtcgatcgc
     3541 tgcgtccaga tctatggcgg ggcaggctat ctggccgaat acccggccga acgcttcttc
     3601 cgcgatgccc ggatcctgcg gatctacgaa gggaccagcc agatcatgca gctgcagatc
     3661 gccaagcacc tgctgcgcga attcgccagc gaaggcgcgg tctggtaagg cgcgatgtac
     3721 gacatcctct caggcctgaa gatcgtcgag gtttcctcgt tcgtcgcttc gcccaccgcc
     3781 gggctctatt gcgcgcagtt cggggccgaa gtggtgcgga tcgaccacaa ggccggcggg
     3841 ctcgattacg accgctacat gatgacgcgc gaaggccgct cgctgtcgtg ggagaacctc
     3901 aaccgcgcca agaaatcggt cgcgcttgac ctgcagagtg cggaagggcg cgaactggcg
     3961 gccgagctgg tgcgtgccac cgggcagatg atcaccaacc tgccggtgaa cagcttcctg
     4021 gcgcacgacg ccctcgccga agggcggccc gacctcgtct cggtgcggat catgggctgg
     4081 cacgacgggc gccaggcgat ggacttcacg gtcaacgcgg ccagcggcta tccgctgatg
     4141 tgcgggccgg aagactggga cccggccacc gcgccgccgg tcaaccagat cctgccggcc
     4201 tgggacttca tcaccggggc ctattgtgcc ttcgcgctgc tggccggcct gcgccaccgc
     4261 gatgcgaccg ggcaggggat cgaaatgcgc gtgccgctgg gcgatgtcgc gatcgggaca
     4321 atggccaacg ccggggccat ggccgaaatg ctgtaccgcg gcgcggaccg cgagcggatc
     4381 ggcaacgcga tctggggcgc cttcggccgc gatttcctga gccgcgacgg caagcgcttc
     4441 atggttgccg cgctgacgcc gaaacagtgg aaggcgacag tcgaagcctt cggcctcagc
     4501 gccgaagtgg ccgcgctgga ggcggaactg ggggtcagtt tcctcgccag cgaccacttc
     4561 cgcttcgtcc accgccaccg cctgttcgac ctgttccagg cggctgcgga ccggttcgac
     4621 tatgccgatc tggc
//
LOCUS       DASUDV010000063         8431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1901784,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000063 DASUDV010000000
VERSION     DASUDV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901784"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFV30_01320"
     CDS             complement(<1..790)
                     /locus_tag="VFV30_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX4846758.1"
                     /translation="MIRFLALLLALLAPLTLARAEPGGPDRVVILIAFDGFRPDYLDR
                     GVTPNLSRLARQGITGPMRPAFPTKTFPNHWTLVTGLVPDRNGIVGNRFEDPARPGEL
                     FTMASDDPFWWNQAEPIWVAAERAGIRTATMFWPGSNVAWGGTKPEGYGPPTGGQRPA
                     DWQMFNQEVSNTQRVNAVLDWLRRPPAIRPRLVTLYFDTVDTAGHRFGPDDPRTTAVI
                     AEADRHMGDLLAGLEELKITADLVIVADHGMAAVSSERVIALDTL"
     gene            complement(860..1960)
                     /gene="ychF"
                     /locus_tag="VFV30_01325"
     CDS             complement(860..1960)
                     /gene="ychF"
                     /locus_tag="VFV30_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX4846759.1"
                     /translation="MGFRCGIVGLPNVGKSTLFNALTETQAAQAANYPFCTIEPNVGN
                     VGVPDPRLDTLAGIAGSAKIIPTQLGFVDIAGLVRGASKGEGLGNQFLGNIREVDAIV
                     HVLRCFENDDIQHVDNRVDPVSDADTVETELMLSDLESLEKRVPAAQKKAAQGDKEAK
                     LLASVLQPALDLLRDGKPARLARPEGEDELRVFNQAQLLTAKPVLYVCNVEEESAANG
                     NAFSEAVFAKAKAEGANAVIVSAAIESELVGMDADERMVFLEEMGLHETGLARVIRSG
                     YDLLHLITFFTVGPKEARAWTVHRGAKAPEAAGEIHSDMQRGFIRAETIAYDDYVTLG
                     GETAAKEAGKLRQEGKEYVVQDGDVLHFKFNV"
     gene            complement(2020..2994)
                     /locus_tag="VFV30_01330"
     CDS             complement(2020..2994)
                     /locus_tag="VFV30_01330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4846760.1"
                     /translation="MPSIDRRTALALGAAATAGAALPASAADKPLAGKSVLITGSSSG
                     FGQLAAMLFARAGAKVFATMRNLPRPAADELRAVAKADKTDITVLELDVLSDASVAKA
                     VAEAEKLAGGGIDILINNAGIGITGPVEVQDLEATKLAFDTNVFGPQRLIRAVLPGMR
                     AKKSGLVFNVSSQLGRVVVPGGGHYSATKFALEALSEQMAYELVPFGIEVCVIQPGGY
                     PTRVWINRNGYTKALKERSNPATLAAYPALTARMGTEDGSGRTADPADVPRAMLEIAG
                     MPAGTRPLRRAVHPGRKPQEAINTVSRETQLAWLGETPFGPWIKAVHD"
     gene            complement(3051..3569)
                     /locus_tag="VFV30_01335"
     CDS             complement(3051..3569)
                     /locus_tag="VFV30_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4846761.1"
                     /translation="MQRVLVIGSPGAGKSTLAHAIAQRTGLPLHHLDRMHWLPGWIER
                     DRAEALERVKAVLVEERWIIDGNYGSTLPVRIPRADTVVWLDYPTHLCLWRVLKRWWQ
                     YRGTARPDMTEGCPENLNAEFLLYVLMFRHNWSARNGAAMAAFSGTLLRFTHPRQTQA
                     WLDRLGTAEGAA"
     gene            3666..5183
                     /locus_tag="VFV30_01340"
     CDS             3666..5183
                     /locus_tag="VFV30_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4846762.1"
                     /translation="MKKLLILPALLALSACADSPEDAFAKAKAGFAARDYVAARVHLA
                     AYLSAHPDNKEALLMQARTLLALGDGDGAQAALAKLTGGKPPAGELAELSAEAALLRG
                     VPDVALDLLGASKGPEAERLRALAALQKNDPGGAQGHFEAALSAGGNARVFADYARFH
                     LIGGAVAEAEAMAAKAEKADPDGIDTLLVLGQLSVRRGDLAKALGYYERASKLYPASL
                     AALIGQAAVLGDLGRHDEMKAVIDRAAGFAPKHPQVVFLKARAAAGQKDWKAVRETVQ
                     PLEAGLAPADPLRVLYGEALLRLGQAELAIAQLQPVLRVAPGNRDAALLLAEALLARG
                     DARQALAVLKPFADSPAARTEELALAAKSAKSAGDPSAAGYEKRAAMPPVQAAGRDLA
                     DADAAMKAGNWAGAAQAYDRILKTTDGRHPLVLNNMAYAQLMLGNLAAARDYADRALK
                     EAPNHPSVLDTAGWVRFKSGKDLQEARRLLRRAAELAPGNPAIRAHLAEAERAGG"
     gene            complement(5194..5910)
                     /locus_tag="VFV30_01345"
     CDS             complement(5194..5910)
                     /locus_tag="VFV30_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cistern family PEP-CTERM protein"
                     /protein_id="HEX4846763.1"
                     /translation="MLARTFMPLTALAALALSAAPAQADVITLGSGSVGQSYTFNFDG
                     FSGSTTIQGLTSLATFTLTAITGNSYTFNYSLTNTTSSPLTSRLSGFGFNTNPDITSA
                     SSTGAFSYTTVGGSYPNGIGAIDVCFKDAKTGSCSGGGSGGLTTGTTGTGSFTLNFSQ
                     PVTSLSLSDFYVRYQSITGAGNITSASGSGTLTGTSGGISTSTSSGGTAVPEPGMLGL
                     FGAALVGLAAMRRRRVPAKA"
     gene            complement(6062..7138)
                     /locus_tag="VFV30_01350"
     CDS             complement(6062..7138)
                     /locus_tag="VFV30_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX4846764.1"
                     /translation="MSSSPIHGRGAQSAAVPQRFGLNAREADGDWLDARDGIDGPGPK
                     LRTSVIEEKPKTILSFNQSPDVPFDRSVNAYRGCEHGCVYCYARPTHAYQDLSPGLDF
                     ETKLFAKPNAAALLRQTLAKPSYRPAPIAMGTNTDPYQPIEGRYRITRQILELCLETG
                     HPVTITTKSARVVRDLDLLTELSQQGLTAVSISVTSLDPKLSGLLEPRASAPAKRLEA
                     LEKLAAAGVPAHVSVAPVIPAITDAFIEGILTEAAARGVQTASWIMLRLPHEVAPLFR
                     EWLEVHFPDRAAKVMGIVQAVRGGRDNDPGFFTRMKPQGPWADLFRHRFRLAARRSGL
                     NREQPRLDCSRFRHPEPDGQYRLF"
     gene            complement(7191..7814)
                     /locus_tag="VFV30_01355"
     CDS             complement(7191..7814)
                     /locus_tag="VFV30_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846765.1"
                     /translation="MDRRLAVLLATLGLTLGACQTLKDPAALAVKAEAARAECALTPA
                     DLAANRTLTWQQFDQQTASPTSWRSLVERKCYNAAAAAYQDYLISGPIPPGERWQTTA
                     RFHLGQSLAHAGRNEEAARIISTARRETEVGGLRWNLYVQGTVAFLTRDRAGLDAAFT
                     ALRAEPGTSNAINAGVLAGLRQCWDKPYIEASAPACVSASGYVMPKD"
     gene            complement(7817..8344)
                     /gene="moaB"
                     /locus_tag="VFV30_01360"
     CDS             complement(7817..8344)
                     /gene="moaB"
                     /locus_tag="VFV30_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX4846766.1"
                     /translation="MAVDPARPFKPIRIAVLTVSDTRDLETDTSGALLAERIQAAGHR
                     LAARAVVKDTVPAITDKLNSWIDDGLADAIITTGGTGLTGRDVTPEALDRIKDKDIPG
                     FGELFRWISYQSIGTSTVQSRACAVLARGVYIFALPGSNGAVKDGWDGILAEQLDSRN
                     RPCNFVELMPRLREV"
BASE COUNT         1279 a         2768 c         3036 g         1348 t
ORIGIN      
        1 ccagggtgtc gagcgcgatc acccgctcgc tcgataccgc ggccatgccg tgatcggcca
       61 cgatcaccag atcggcggtg atcttcagct cctccagccc ggccagcaga tcgcccatgt
      121 ggcggtcggc ctcggcaatc accgccgtcg tgcgcggatc gtcggggcca aagcggtggc
      181 cggcggtgtc gaccgtgtcg aaatagagcg tgacgaggcg cgggcggatc gcgggcgggc
      241 ggcgcagcca gtccagcacg gcgttgaccc gctgggtgtt gctgacctcc tggttgaaca
      301 tctgccagtc ggccgggcgc tggccgccgg tgggcgggcc atagccttcg ggcttggtgc
      361 cgccccaggc cacgttggag ccgggccaga acatcgttgc ggtgcggatc ccggcgcgtt
      421 cggcggccac ccagatcggt tcggcctggt tccaccagaa cggatcgtca ctggccatgg
      481 tgaacagctc gcccggccgc gccgggtctt cgaagcgatt gccgacgatc ccgttgcgat
      541 cgggcaccag cccggtcacc agcgtccagt ggttgggaaa ggtcttggtc ggaaaggcag
      601 ggcgcatcgg cccggtgatg ccctgccggg ccagccgcga caggttgggg gtgaccccgc
      661 ggtctagata gtccgggcgg aacccgtcga acgcgatcag gatcaccacc cggtccggcc
      721 cgcccggctc ggcgcgggcc agggtcagcg gggccagcag ggcaagcagc agggccagga
      781 agcggatcat ggcgcaagct tagccgcgat ccccgcccat gcaatcaccc gttcgcgccg
      841 ggcgggactt gcgccgcaat cagacgttga acttgaagtg cagcacgtcg ccgtcctgca
      901 ccacgtattc cttgccttcc tggcgcagct tgccggcttc cttggccgcg gtttcgccgc
      961 ccagcgtgac atagtcgtca taggcgatcg tttcggcccg gatgaagccg cgctgcatgt
     1021 cggagtggat ctcgcccgcg gcttccgggg ccttggcgcc gcggtgcacg gtccaggcgc
     1081 gcgcttcctt ggggccgacg gtgaagaagg tgatcaggtg gaggaggtca tagcccgagc
     1141 ggatgacgcg ggcgaggccg gtttcgtgaa ggcccatttc ctcaaggaag accatccgct
     1201 cgtcggcgtc catgccgacc agctcgcttt cgatcgcggc ggagacgatc acggcgttgg
     1261 cgccttcggc cttggccttg gcaaagaccg cttcggaaaa ggcattgccg ttcgccgcgc
     1321 tctcttcctc gacgttgcag acatagagca ctggcttggc ggtgagcagc tgggcctggt
     1381 tgaagacccg cagctcgtcc tcgccctcgg gccgggcgag gcgcgcgggc ttaccgtcgc
     1441 gcagcagatc gagcgcgggt tgcagcaccg aggcgagcag cttggcctcc ttgtcgccct
     1501 gggcggcctt cttctgggcg gcggggaccc gcttttcgag gctttcgagg tcggacagca
     1561 tcagctcggt ctcaaccgtg tcggcgtccg aaaccggatc gacgcggttg tcgacgtgct
     1621 ggatgtcgtc gttctcaaag cagcgcagca cgtggacgat cgcgtccact tcgcggatgt
     1681 tgccgagaaa ctggttgccc aggccttcgc ccttcgacgc gccgcgcacc aggccggcga
     1741 tgtcgacaaa gcccagctgg gtcgggatga tcttggcgct gccggcaatc ccggccagcg
     1801 tgtccagccg cggatcgggc acgccgacgt tgccgacgtt gggttcgatc gtgcagaacg
     1861 gatagttcgc cgcctgcgcc gcctgcgtct cggtcagcgc gttgaacagg gtggacttgc
     1921 cgacattcgg caggccaacg ataccgcaac gaaaacccat cactcactcc gcttcaggct
     1981 aggcgcgggc ccatagcggg ggcggggggt tttggcgagt cagtcgtgca ccgccttgat
     2041 ccacgggccg aacggcgttt cgcccagcca ggccagctgg gtctcgcggc tgacggtgtt
     2101 gatcgcctcc tgcggcttgc gtccggggtg gacggcgcgg cgcagcgggc gggttccggc
     2161 gggcatgccg gcaatctcca gcatggcccg cggcacgtcg gcgggatcgg cggtgcggcc
     2221 agagccgtcc tcggtcccca tccgcgcggt cagcgccgga taggcggcca gagtggcggg
     2281 attgctccgc tccttcagcg ccttggtata gccgttgcgg ttgatccaga cccgggtcgg
     2341 atagccgccg ggctggatca cgcagacctc gatcccgaag ggcaccagct cataggccat
     2401 ctgctcgctc agggcttcca gcgcgaactt ggtcgccgaa tagtgcccgc cgcccggcac
     2461 cacgacccgg cccagctgcg aggacacgtt gaacaccagc ccgctcttct tcgcgcgcat
     2521 ccccggcagg accgcgcgga tcagccgctg cgggccgaac acgttggtat cgaaggccag
     2581 cttggtcgct tccaggtcct gcacctcgac cgggccggtg atgccgatcc cggcgttgtt
     2641 gatcaggatg tcgatcccgc cgccggccag cttttccgcc tcggccacgg ccttcgccac
     2701 cgaggcgtcg gacaggacgt ccagctccag cacggtgatg tcggtcttgt cggccttggc
     2761 caccgcgcgc agttcgtcgg cggcggggcg gggcaggttg cgcatggtcg cgaagacctt
     2821 ggccccggcg cgggcgaaca gcatcgccgc cagctggcca aagccggagg acgatcccgt
     2881 gatcagcacg ctcttcccgg ccagcggctt gtcggcggcg gaggcgggta gggcggcccc
     2941 ggcggtggcg gcggccccca gggccagcgc ggtgcggcgg tcgatcgaag gcatcgtggt
     3001 ctcccgggtt tgtctgtctg cggccaagct agggtgcgtg cccgcggggt tcaagccgcc
     3061 ccttcggccg tgcccagccg gtccagccag gcctgcgtct ggcgcgggtg ggtgaagcgc
     3121 agcagggtgc ccgaaaaggc cgccatcgcc gcaccgttgc gggccgacca gttgtgccgg
     3181 aacatcagga catagagcag gaattcggcg ttaaggtttt ccgggcagcc ctcggtcatg
     3241 tcgggccggg cggtgccgcg gtattgccac cagcgcttca gcacgcgcca caggcacagg
     3301 tgagtggggt aatccagcca gaccaccgta tcggcgcggg ggatgcggac cggcagggtc
     3361 gatccgtaat tgccgtcgat gatccagcgc tcttcgacca gaaccgcttt cacccgctcc
     3421 agcgcctcgg cgcggtcgcg ctcaatccag ccgggcagcc agtgcatccg gtcgaggtgg
     3481 tgcagcggca gcccggtgcg ctgcgcgatg gcatgggcca gggtggactt gcccgctccg
     3541 ggagagccga tgacgaggac gcgctgcatg ggcgcgctct agccaagccg gagcctggcg
     3601 aacagggcca ctcgatgaac gccttttcac cgaaatttca ggctttgctg ctatttccgc
     3661 ggaccatgaa gaagctgctg atcctgcccg ctctgctcgc cctttcggcc tgcgccgaca
     3721 gccccgaaga cgcctttgcc aaggccaagg ccggctttgc cgcgcgggac tatgtagcgg
     3781 cgcgggtcca tctcgcggcc tatctctcgg cccatccgga caacaaggaa gccctgctga
     3841 tgcaggcccg caccctgctg gcgctgggcg acggcgacgg ggcccaggct gcgctggcca
     3901 agctgaccgg gggcaagccg ccggccgggg aactggccga actgtccgcc gaagccgcat
     3961 tgctgcgcgg tgtgcccgat gtggcgctgg atctgctggg ggcgagcaag ggcccggagg
     4021 ccgaacgcct gcgcgcgctg gccgcgctgc agaagaacga tccgggcggg gcccagggcc
     4081 acttcgaagc cgccctttct gctggcggca acgcgcgggt ctttgccgat tacgcccgct
     4141 tccacctgat cggcggcgcc gtggccgagg ccgaggccat ggcggcgaag gccgaaaagg
     4201 ccgatccgga cgggatcgat acgctgctgg tgctgggcca gctttcggtc cggcgcggcg
     4261 atctggccaa ggcgctgggt tactacgaac gcgcgtccaa gctctatccc gccagccttg
     4321 ccgcgctgat cggccaggcg gcggtgctgg gcgatctggg ccggcacgac gaaatgaagg
     4381 ccgtgattga ccgcgccgcc ggctttgcgc cgaagcaccc gcaggtcgtg ttcctgaagg
     4441 cccgcgccgc ggccgggcag aaggactgga aagccgtgcg cgaaacggtc cagccgctgg
     4501 aggccgggtt ggccccggcc gatccgctgc gggtgcttta tggcgaggcg ctgctgcggc
     4561 ttggccaggc ggaactggcg attgcccagc ttcagccggt gttgcgggtt gctcccggca
     4621 accgcgatgc ggccctgctg ctggccgagg cgctgctggc ccggggcgat gcccgccagg
     4681 cgctggcggt gctgaagcct tttgccgaca gcccggctgc ccgcaccgaa gaactggcgc
     4741 tggccgccaa gtccgccaag agcgcgggcg atccctctgc cgcgggctat gagaagcgcg
     4801 ccgcgatgcc cccggtccag gcggccgggc gcgatctggc cgatgccgat gcggcgatga
     4861 aggccgggaa ctgggccggc gcggcccagg cctatgaccg gatcctcaag accaccgacg
     4921 ggcgccaccc gctggtgctc aacaacatgg cttatgcgca gctgatgctg ggcaacctgg
     4981 ccgccgcgcg cgactatgcc gaccgcgcgc tgaaggaagc gccgaaccat ccctccgtgc
     5041 ttgataccgc cgggtgggtg cggttcaagt ccggcaagga cctgcaagaa gcccggcgcc
     5101 tgctgcgccg tgcggcggag ctggcgccgg gcaatccggc gatccgtgcg cacctggcag
     5161 aggccgagcg cgccggagga taattcctta ggctcaggcc ttggcgggga cccggcggcg
     5221 gcgcatcgcg gccagaccga ccagcgccgc accgaacagg cccagcatgc ccggttccgg
     5281 aacggccgtg ccgcccgacg aggtcgaggt gctgatcccg cccgacgtgc cggtgagcgt
     5341 gcccgagccg ctggccgagg tgatgttgcc ggccccggtg atcgactggt agcggacgta
     5401 gaagtcgctc agcgacagcg aggtcaccgg ctgcgagaag ttgagggtga agctgccggt
     5461 gccggtcgtc ccggtggtca ggccgccgct gccgccgccc gagcaggagc cggtcttggc
     5521 atccttgaag cagacgtcga tcgcgccgat cccgttggga taggagccgc cgaccgtggt
     5581 gtagctgaag gctccggtcg agctggcgct ggtgatgtcc gggttggtgt tgaagccaaa
     5641 gcccgacagc cgcgaggtca gcgggctgct ggtcgtgttg gtcaggctgt agttgaaagt
     5701 gtagctgttg ccggtgatcg ccgtcagcgt gaaggtcgcc agcgaagtca gcccctgaat
     5761 ggtggtgctg cccgagaaac cgtcaaagtt gaaggtgtag gactggccca ccgatccgct
     5821 gcccagcgtg atcacgtcgg cctgggcggg agcagcggag agtgccagcg cggccagggc
     5881 ggtgagcggc atgaaagtgc gagcaagcat cggggaaacc ttcttcagac agcgttgtta
     5941 acgcgtcacg gagcaatagc cgtgccaatc ctgattttcc gcgaaggagc atggttaacg
     6001 ccggcccaac aggcgcgtct ttgtaaagta aaccgacgct ttccggggct tttctgccgg
     6061 atcaaaacag ccggtactgg ccatccggtt cgggatggcg aaagcggctg caatcgagcc
     6121 ggggctgctc ccggttgagg ccgctgcggc gggcggcgag ccggaaccgg tggcggaaca
     6181 ggtcggccca ggggccttgc ggcttcatcc gggtgaagaa cccgggatcg ttgtcacgcc
     6241 cgccgcgcac cgcctggacg atccccatca ccttggcggc gcggtctgga aaatggacct
     6301 cgagccattc gcggaacagc ggcgcaacct cgtgcggcag gcgcagcatg atccagctgg
     6361 cggtctgcac cccgcgcgcg gcagcctcgg tcaggatgcc ctcgataaag gcatcggtga
     6421 tggcgggaat gaccggggcg accgagacgt gcgccggcac gcccgccgcg gccagctttt
     6481 ccagcgcctc cagccgcttg gccggcgcac tggcccgggg ctcgagcagg ccggagagct
     6541 tgggatcgag gctggtgacc gagatcgaca ccgcggtcag gccctgctgg gagagctccg
     6601 tcaacagatc cagatcgcgc accacccgtg cgctcttggt ggtgatcgtc acggggtggc
     6661 ctgtttcgag gcacagctcc agaatctggc gggtgatccg atagcggccc tcgatcggct
     6721 gatagggatc ggtgttggtg cccattgcaa tcggcgcggg cctgtaggac ggcttggcca
     6781 gcgtctggcg cagcagggcc gcggcgttcg gcttggcgaa gagcttggtc tcgaaatcca
     6841 gcccggggga caggtcctga taggcatggg tggggcgggc atagcaatag acgcagccgt
     6901 gctcgcagcc gcgataggcg ttgaccgacc ggtcgaacgg cacgtcgggt gactggttga
     6961 aggacaggat ggtcttcggc ttttcctcga tcaccgaggt gcgcagtttc ggccccggac
     7021 cgtcgatccc gtcgcgcgca tccagccaat ccccatccgc ctcgcgcgcg ttcagcccga
     7081 aacgctgcgg caccgccgcc gactgcgcgc cgcggccgtg gatgggggat gaggacatcc
     7141 ccagatgtga acataggaag aacaaaaagg caagacggct ttcgccgggc ctagtccttg
     7201 ggcatcacat agccgctggc actgacgcag gcgggtgcgg acgcttcgat gtaaggcttg
     7261 tcccagcatt ggcgcaggcc ggcgagcaca ccggcgttga tggcgttgga cgttcccggt
     7321 tctgcgcgca gcgcggtaaa ggcggcgtca aggccggcgc ggtcgcgcgt caggaaggcc
     7381 accgtgccct gaacgtagag gttccagcgc aggccgccca cttcggtctc gcggcgggcc
     7441 gtggagatga tccgggcggc ctcttcgttg cgtccggcat gggccagcga ttggcccagg
     7501 tggaaccggg cggtcgtctg ccagcgctcg ccgggcggta tcgggccgga gatcaggtaa
     7561 tcctgatagg ccgcggcggc cgcgttgtag cacttgcgct ccaccagcga gcgccagctg
     7621 gtcgggctgg cggtctgctg atcgaattgc tgccaggtga gcgtgcggtt cgcggcgaga
     7681 tcggccgggg tcagagcgca ttcggcgcgg gcggcctcgg ccttgacagc cagcgcggcg
     7741 ggatccttga gcgtctggca agcgccgagc gtcaagccca gggttgccag caagaccgca
     7801 aggcgccggt ccatctctag acctcccgca gccgcggcat cagttcgacg aaattgcagg
     7861 ggcggttgcg gctgtcgagc tgttcggcca ggatcccgtc ccagccgtcc ttcaccgcgc
     7921 cgttcgatcc gggcagggcg aagatgtaaa ccccgcgggc cagcaccgcg caggcgcggc
     7981 tctggacggt cgaggtgccg atcgactggt agctgatcca gcggaacaac tcgccgaagc
     8041 cggggatgtc cttgtccttg atccggtcga gcgcttcggg ggtgacgtcg cggccggtca
     8101 ggccggtgcc gccggtggtg atgatcgcgt cggccaggcc gtcgtcgatc cacgaattga
     8161 gcttgtcggt gatggccgga accgtgtcct tcaccaccgc gcgggcggcg agccggtgcc
     8221 cggcggcctg gatccgctcg gccagcagcg cgcccgaggt gtcggtctcc agatcgcggg
     8281 tgtcggacac cgtcagcacg gcgatccgga tcggtttgaa cgggcgcgcc ggatcaacgg
     8341 ccactttgca gggtcccgtc gggccgcatc cgcacggcga ccgcgccggt caggcccggg
     8401 aaagtcggcc acatgccctg ggccagcgcc a
//
LOCUS       DASUDV010000064         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1703123,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000064 DASUDV010000000
VERSION     DASUDV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703123"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..959)
                     /locus_tag="VFV30_01365"
                     /pseudo
     CDS             complement(1..959)
                     /locus_tag="VFV30_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683653.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            1386..2213
                     /locus_tag="VFV30_01370"
     CDS             1386..2213
                     /locus_tag="VFV30_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4846767.1"
                     /translation="MSVINTNISAIKAANASNSANKMLGTAMERLSTGKRIGSAKDDA
                     AGLAIATSMTAQVRGMNQAIRNSNDGIALAQTAEGALSEVTNMLQRVRELAVQSASGT
                     YQDATDRAYMQSEVDELTAQIDQVITNSKFNGVNLFDGTTATVTIQTGADGADTVDLT
                     MADLTSLAASGGAAGSYDVSSATAANTLLGTIDTELDSINSARAGLGAGQNRLESVVN
                     NLTSTSTNLADARSRIEDADYSAETTAMAKAQILSQASTAMIAQANQSQQNVLSLLR"
     gene            2304..>2921
                     /locus_tag="VFV30_01375"
     CDS             2304..>2921
                     /locus_tag="VFV30_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4846768.1"
                     /translation="MLSSLTPQPADALLALIKLYAADPRTDKIDLGVGVYRTGQGDTP
                     VFGAIKAAEQKLVDTQESKSYLGPEGDMGFVHALMPYVFGAQDPTMGGRIEGMQTPGG
                     TGGVRLAVALAKRSGAPKIWMGTPSWPNHAQIFADIGLELKTFTHATADGVVDMDALR
                     AAIAAADEGDAILLHGCCHNPSGIDYSEAQWDEIAGLVAEAKLLPV"
BASE COUNT          529 a          970 c          981 g          441 t
ORIGIN      
        1 gatgccaagc tcgcgtgcag ccgcgaggcg attgccgcca accgccgcaa gcacttccat
       61 gctcgcttca gcctcggact gctggaccac ggcggagagc cggcgcggct ggtcctggac
      121 cagggccggg gcagaggcga ggcgggcggg ctgatcgaac aggatctgcg ccggaccgat
      181 ttcctcgccg ccatcggcca gcaaaagtgc acgtcggatc acgttttcca gctcgcggac
      241 attgccgggc caggcatgga gcttgagctt ggccagcgcc gcctcgccca gccagggcag
      301 tgcgaggccc tgcggggtgt ggcgcagcac catggcgaag gccagcgggg cgatatcctc
      361 gggccgttcc cgcaaggggc gtagcgcgag ggggaagacg ttgaggcggt agaacaggtc
      421 ttcgcggaac cggccggccg cgacctcact cggcagatcg cggttggcag cagcgatgat
      481 ccgaacatcg accttgatcg gccgggtcgc gccgatcggc acaacctcac cctcctgcag
      541 cgcccgcagc agcttggcct ggagcgccag tggcatctcg gcaatctcgt ccagcagcag
      601 cgtgccgcca tcggccgcgc ggaagaagcc ttcgctggcc tgggtcgcgc cgtgaaggcg
      661 cccttctggt ggccgaacag cagggcttcg agcatggctt cgggcatggc ggcacagttg
      721 acggccacga aggggccatc ccgacgcggg ctgtgatcgt ggatgaagcg gctcagcact
      781 tccttgccgg tgccggtcgg cccggcgatc agcacgggga tctcgctggc cgcaaggcgt
      841 tcggccagcg ccaggaccga aaggctttcg ggatcggccg caatcggtgc gccaggggca
      901 gtcagcgcgg cgagggcggc ggccagcgag gcctcgcccg aggggtcgcg ataggtcagg
      961 tggaaccgcc cgcgcccgtc aagccggcgg agctgcggct gggccgcccg ctccagcgtc
     1021 agggccgggg ccgggccgac tggcgccgga tcctgggcgg tgcgcaggat gcgggcgggg
     1081 ccatggccgc ttgcgcccag caaggcttca agccgctggg ccagggcaac atgctgtcgc
     1141 ttcaccgtat cgctaaaggc caaggccgtc atttgcggta acccccgagt taaccattcc
     1201 ttcgcgagca ttcatgcgcg attccaagga aacgggtcgc aaacggcggc atcaggttgg
     1261 ttacctaggt aggccggggc gcaacgaggg gtgcttaatt tccggcccga cccgccgctc
     1321 taggggctgg cagccgcacc ggatggtccg ctggcggttt gcgagactac ttgggagtac
     1381 cgacgatgag tgtcatcaat accaacatca gcgcgatcaa ggctgccaac gcctcgaact
     1441 cggccaacaa gatgcttggc accgccatgg agcggctttc gaccggcaag cgcatcggca
     1501 gcgccaagga cgacgcggct ggcctcgcca tcgccaccag catgaccgcc caggtccgcg
     1561 gcatgaacca ggcaatccgc aattcgaacg acggcattgc cctggcgcag accgccgaag
     1621 gtgcgctgag cgaagtcacc aacatgttgc agcgcgttcg cgaactggct gtccagtcgg
     1681 cctcgggcac ctatcaggat gccaccgacc gcgcctatat gcagtcggaa gttgacgagc
     1741 tgaccgcgca gatcgatcag gtcatcacca acagcaagtt caacggcgtc aacctgttcg
     1801 atggcaccac tgccacggtc acgatccaga ccggtgccga cggcgccgat acggttgacc
     1861 tgaccatggc cgatctcacc tcgctggcag ccagcggcgg tgcggcgggt tcgtacgacg
     1921 tgtcgagcgc gacggcggcc aacaccctgc tgggcacgat cgacaccgaa ctcgattcga
     1981 tcaactcggc ccgcgctggc ctcggtgccg gccagaaccg tctggaatcg gtggtcaaca
     2041 acctcaccag cacctcgacc aacctggccg atgcccgcag ccggatcgag gatgccgact
     2101 attcggccga aacgaccgcg atggccaagg cccagatcct ctcgcaggct tcgaccgcga
     2161 tgatcgccca ggccaaccag agccagcaga acgttctttc gctgctgcgc taagccttaa
     2221 ggcaacaggg ggaggggccg tgccatgcgc gcggcccctt tcgcttgccc cgcgcaaaag
     2281 tgggggttaa gggccgccgc atcatgctca gctccctgac tccccagccc gccgatgcgc
     2341 tgcttgcgct gatcaagctc tatgccgccg atccgcgcac cgacaagatc gaccttggcg
     2401 tcggcgtcta ccgcaccggt cagggcgaca ccccggtatt cggcgcgatc aaggccgcgg
     2461 aacaaaagct cgtcgatacc caggaatcga agagctacct cgggcctgaa ggcgacatgg
     2521 gttttgtcca tgcgctgatg ccttatgtct ttggcgcgca ggacccgaca atgggcggcc
     2581 ggatcgaagg catgcagaca ccaggcggca ccggcggcgt gcgccttgcg gtggcactgg
     2641 ccaagcgttc gggcgcaccg aagatctgga tgggcacgcc aagctggccc aaccacgcgc
     2701 agatctttgc cgacatcggg ctggagctga agaccttcac ccacgcgacc gctgatggtg
     2761 tggttgatat ggacgcgctg cgcgccgcga tcgctgcggc cgatgaaggc gatgcgatcc
     2821 tgctgcacgg ctgttgccac aaccccagcg gcatcgatta cagcgaagcg cagtgggacg
     2881 agatcgccgg gctggtcgct gaagccaagc tgctgcccgt g
//
LOCUS       DASUDV010000065         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1548480,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000065 DASUDV010000000
VERSION     DASUDV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548480"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2063)
                     /locus_tag="VFV30_01380"
     CDS             complement(<1..2063)
                     /locus_tag="VFV30_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX4846769.1"
                     /translation="MTRRASAHSDSRIKAVLGPTNTGKTHLAIERLCAHSSGAIGFPL
                     RLLAREVYDKVCKIKGVDRVALITGEERIEPRHARWLLCTAEAMPVGADLSFVGIDEA
                     QLSADRERGHIFTDRLLHARGRDETMILGSATIEPLVRALVPQAEIVTRPRFSTLSHV
                     GAKKLSRVPPRSAIVAFSAEQVYAMAEMLRRFRGGAAVVMGALSPETRNRQVELYQAG
                     EVDYLVATDAIGMGLNLDIEHVAFAGLSKFDGQRQRRLTPAEMAQIAGRAGRHQKDGS
                     FGTLAGPGGREAEFSDEEVYAIEEHRFAPLTRLFWREAEPDCTSLHSLIADLERAPER
                     PELAPAPEAIDLAVIKRLAEDPEIAGSLRGEAAVRRFREVCSLPDFRQHGAETHSRFV
                     ARLWQDLQRGQLGQDFVAKAISDLDNPAGDIDTLQGRIASVRSWAYIAQRPDWVLARE
                     EMAARARAAEARLSDALHARLTERFVNRRTAVLMKKLGGDASLLPVRLVDDEVLVDGE
                     HIGVLAGFRLRIDPQARLADRKLLLAAAERHLPALLGERAARLGEALATASPDLSLDG
                     ARICWEGEVVAQLAKGRGLLSPRIELDAALGALPALTRQSLQAGLDQWLGHALAALAP
                     LTALEAASRQEQAGPELRALLIRLVEAGGTLERTGSGLERLSETQRGQLRRLGVRIGA
                     LDLFMP"
     gene            2225..>3694
                     /locus_tag="VFV30_01385"
     CDS             2225..>3694
                     /locus_tag="VFV30_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4846770.1"
                     /translation="MTKRGEEAGWAAGTAMARAMAIPAGAIALAPTRAERLMRLREVL
                     AQRFAHIEISYDLAEDIGSAPWFRGLGLMLGLGLFASLFWHDSTTIAAPPSMVIDQDQ
                     RDEFRSQMIMPLALGADSGRRMGATSALVALAAAPERPTLQMVATLGQGDSLSRMLVR
                     AGVDSGDALTAAGLVAMVMPKGEVAPGTQADLTLGKIDPATGTRPLERMAMRARFDLD
                     LALTRTPSGQLTIQPHAIAVDTTPLRIRGTVGTSIYRSARAAGAPIEAIQQYLQVLDQ
                     HLSLDTQIANGDTFDLIVSYKRSARGEAQVGELLFAGLERDGKPQAQLLRWGKDGQFF
                     EASGMGAQRTGLIMPVVGRITSNFGARRHPVLGYTRMHAGVDFGAAHGSPIYAVGDAR
                     VIYSGWHGGHGNYVKLDHGGGYATAYAHMSRIAVSSGSQVRTGQIIGYVGSTGLSTGP
                     HLHYELYRNGQAVNPLSVRFTVSSQVDPAELKAYKARMAA"
BASE COUNT          562 a         1256 c         1268 g          608 t
ORIGIN      
        1 ggcatgaaca gatcgagcgc gccaatcctg acgccaagcc gtcgcaactg accgcgctgg
       61 gtttcgctca gccgctccag cccggacccg gtgcgctcaa gtgtgccacc ggcttcgacc
      121 aggcggatca gcagtgcgcg aagttccgga ccggcctgtt cctgccgcga ggcagcctcg
      181 agcgcggtga gtggggcgag ggccgccagt gcatgcccca accattgatc gaggccggcc
      241 tgcagcgact ggcgggtgag tgcgggcaag gcgcccagcg cggcatccag ctcgatccgg
      301 gggctgagca ggccgcgtcc cttggcgagc tgggccacca cttcgccttc ccagcagatc
      361 cgcgcgccat cgagggataa gtccggtgat gccgtggcca gcgcttcgcc cagccgggcg
      421 gcgcgttcgc ccagcaatgc tggcaagtgc cgttccgccg ccgccagcag cagcttgcgg
      481 tcggccagcc gcgcctgggg atcgatccgc agccgaaagc ctgccagcac gccaatgtgt
      541 tcgccatcga ccagcacttc gtcatcgacc agtctgaccg gcagcaggct ggcatcgccg
      601 cccagcttct tcatcagcac cgcggtgcgg cgattgacga agcgttcggt cagccgcgcg
      661 tgcagggcat cggagaggcg cgcttcggcg gcgcgggccc gcgctgccat ttcctcgcgc
      721 gccagcaccc agtcggggcg ctgggcgata taggcccagc ttcgcaccga ggcgatccgt
      781 ccttgcagcg tgtcgatatc gcctgccgga ttatcgagat cggagatcgc ctttgccacg
      841 aaatcctggc ccagctggcc gcgctgcagg tcctgccaca gccgggccac gaagcgcgaa
      901 tgggtttcgg cgccgtgttg gcgaaaatcg ggtagcgagc agacttcgcg gaaccggcgc
      961 accgccgctt cgccgcgcag gctcccggca atttcgggat cttcggccag gcgcttgatc
     1021 accgccagat cgatcgcttc gggggcgggg gccagttcgg gccgttcggg cgcgcgttcg
     1081 aggtcggcga tcaggctgtg caggctggtg caatcgggct cggcctcgcg ccagaacagc
     1141 cgcgtcagcg gagcgaagcg gtgctcctcg atcgcgtaga cttcctcgtc gctgaactcg
     1201 gcctcgcgcc cgcccgggcc ggccagtgtg ccgaagctgc cgtccttctg gtggcgcccg
     1261 gcgcgcccgg cgatctgggc catttcggcc ggggtcaggc ggcgctggcg ttggccgtcg
     1321 aacttcgaca ggccggcgaa ggcaacatgt tcgatatcga gattgagtcc catgccgatc
     1381 gcgtcggtcg ccaccagata gtcgacctcg cccgcctggt agagctcgac ctggcggttg
     1441 cgggtttcgg gcgagagcgc gcccatcact accgccgcgc cgccgcggaa ccggcgcagc
     1501 atttcggcca tggcatagac ctgctcggcc gagaaggcga cgattgcgct gcgcggtggc
     1561 acccggctga gcttcttcgc cccgacatgg ctgagcgttg aaaaacgcgg tcgggtgacg
     1621 atttcggcct gcggcacgag cgccctgacc agcggctcga tcgtcgccga gcccaggatc
     1681 atcgtctcgt cgcggccgcg tgcgtgcagc agccggtcgg taaatatgtg gccgcgttcg
     1741 cgatcggcgc tcaactgggc ctcgtcgatc cccacgaacg aaagatcggc gcccaccggc
     1801 atcgcctcgg cggtgcacag cagccagcgc gcgtgcctgg gctcgatccg ttcctcgccg
     1861 gtgatcagtg caacccggtc cacacccttg atcttgcaga ccttgtcgta gacttcgcgg
     1921 gccagcagtc gcagtggaaa gccgatcgcg cccgatgaat gggcacaaag ccgctcgatc
     1981 gcgagatggg tcttgccggt gttggtgggg cctagcaccg ccttgatgcg actatccgaa
     2041 tgagcgcttg cacggcgggt catgacatcg gccaatgtgg cgtctcgccg ccgcgatgca
     2101 aggcggcctg cgccttcgtc cccagccagg cgcgtgcccg gcgattttcg cgcccgtctt
     2161 aggcgaagat taaccacctt tcgggcaagg atttgggcag gctgcagctg ccggggacgt
     2221 gtttgtgaca aagcggggcg aagaggccgg ttgggccgcg ggaacggcca tggcgcgggc
     2281 catggcgatc ccggcgggtg cgatcgcgct tgcccccacc cgggccgagc gcctgatgcg
     2341 gttgcgcgaa gttctggcgc agcgtttcgc ccatatcgaa ataagctatg atctggccga
     2401 ggacattggc agcgcgccct ggttccgcgg gcttgggctg atgctgggcc tgggtctgtt
     2461 cgcgagcctg ttctggcacg attcgaccac gatcgccgcg ccgcccagca tggtcatcga
     2521 ccaggaccag cgcgacgagt ttcgcagcca gatgatcatg ccgctggcgc tgggtgccga
     2581 tagcgggcgg cggatggggg caaccagcgc gctggttgcg ctggctgccg cgccagagcg
     2641 cccgacgctg caaatggtgg caacgctggg ccagggcgac agcctgagcc ggatgctggt
     2701 tcgagccggg gtcgacagcg gcgatgccct gaccgccgcc ggactggtgg cgatggtcat
     2761 gcccaagggc gaggttgccc ccgggacgca ggccgacctg accctgggca agatcgatcc
     2821 ggccaccggc acccgcccgc tcgaacgcat ggcgatgcgc gcccgcttcg atctcgatct
     2881 cgcgcttacc cgcacccctt ccgggcagct gacaatccag ccccatgcca tcgcggtcga
     2941 caccaccccg ctgcgcattc gcggcaccgt tggcacctcg atctatcgct cggcgcgggc
     3001 cgccggggcg ccgatcgagg caatccagca atacctccag gtgcttgacc agcacctcag
     3061 cctcgatacc cagatcgcca acggcgacac ctttgacctg attgtttcct acaagcgctc
     3121 ggcgcgcggc gaggcgcagg tgggcgagct gctgttcgcc gggctcgagc gcgatggcaa
     3181 gccgcaggcg cagctgctgc gctggggcaa ggacgggcag tttttcgaag cctccggcat
     3241 gggcgcgcag cgtaccggcc tgatcatgcc ggtggttggc cggatcacct cgaacttcgg
     3301 ggcgcggcgc catccggtgc taggctatac ccggatgcac gctggggtcg atttcggcgc
     3361 cgcgcacgga tcgccgatct atgcggtcgg cgatgcccgg gtgatctatt cgggctggca
     3421 cggcgggcat ggcaactacg tcaagctcga ccatggcggc ggctatgcca cggcctatgc
     3481 gcacatgagc cggatcgccg tttcttcggg ctcgcaggtc cgcaccgggc agatcatcgg
     3541 ctatgtcggc tccaccgggc tgtcgaccgg gccgcacctg cactacgagc tttaccgcaa
     3601 tggacaggcg gtcaatccgc tgtcggtgcg cttcaccgtc tccagccagg tcgatccggc
     3661 cgagctcaag gcctacaagg cgcggatggc ggcc
//
LOCUS       DASUDV010000066         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_90489,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000066 DASUDV010000000
VERSION     DASUDV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90489"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1228)
                     /gene="argC"
                     /locus_tag="VFV30_01390"
     CDS             complement(305..1228)
                     /gene="argC"
                     /locus_tag="VFV30_01390"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX4846771.1"
                     /translation="MTHTVFIDGAAGTTGLEIVERLAPRSEFELVILDEQQRKSAEAR
                     RAAYHAADFAVLCLPDEAAIEAVALAEGSKVRIIDASSAHRVAPGWTYGFPELVGRER
                     VAEASRVSNPGCYPTGFLALLAPLIRHGLLPSDWPYTCHAVSGYSGGGKALIARFEDD
                     RDIAFRDYGLALGHKHVPEMREYAGLAHDPLFAPAVIPAHRGMVVEVPLHIGLMERAA
                     PTDGLRDCLVDFYAGSPVVTVHQETPDELLLRQAMAPSDRLELWVFGSRDGRQARLVA
                     RLDNLGKGASGAAVQSLNLMAGLDETAGLSL"
     gene            complement(1366..1707)
                     /locus_tag="VFV30_01395"
     CDS             complement(1366..1707)
                     /locus_tag="VFV30_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX4846772.1"
                     /translation="MTATAIPTGQLALSGPAEKLDAAHWPVRGDLAHIRLAGRCFVPH
                     YAVPMEREVVPCGAKLFALNSKDSDVRDTLGGGTLFNVLDVTGGWAWGQVGEDGFVGY
                     LPVEALQERRG"
     gene            complement(1769..>2101)
                     /locus_tag="VFV30_01400"
     CDS             complement(1769..>2101)
                     /locus_tag="VFV30_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX4846773.1"
                     /translation="PALFTRRDETADELLKAGEAADDRCWRLPLFDGYREYLKSDIAD
                     LNNSGSNGFAGASVAGLFLDRFVPEGTDWAHFDTFAWRPAAKPGRPKGGDALGLRAAW
                     GLLKGRYG"
BASE COUNT          350 a          711 c          702 g          338 t
ORIGIN      
        1 agcttgaacg gcttgatgat cgcttcgatc tttttcacgt gctcattccc ctgcgcagca
       61 agccggatcc gctttttcgc ctcggcgaag gcgcggcccg gagtttgtct ttgccactgt
      121 cccaatcctt atcaagaatc gtgccagagg cctgcacaag gccctaggcc atggtcaggg
      181 aatggaaaag ggcggaatcg caagatttga atcggtgatg gtcaccttgc gaaacaatcg
      241 caatgccgag gcgtggtcag gcttgcctga tttttcggca agcgctgcct gtttttcggg
      301 caggctagag cgaaagcccc gccgtttcgt ccagcccggc catcaggttg aggctctgca
      361 ccgccgcgcc gctggcgccc ttgcccaggt tgtcgagccg cgcgacgagg cgcgcctggc
      421 gtccgtcgcg gctgccgaag acccacagct ccagccggtc ggacggggcc atggcctggc
      481 gcagcagcag ctcgtccggg gtttcctggt ggacggtgac caccgggctg ccggcataga
      541 aatcgaccag gcaatcgcgc aggccatcgg tcggcgcggc gcgctccatc aggccaatgt
      601 gcagcggcac ttccaccacc atgccgcggt gcgccgggat taccgccggg gcgaacagcg
      661 gatcgtgggc cagcccggcg tattcccgca tctccggcac gtgcttgtgg cccagcgcca
      721 ggccgtaatc gcggaaggca atgtcgcggt cgtcctcgaa ccgggcgatc agcgccttgc
      781 cgccgcccga atagccggac accgcatggc aggtgtaggg ccagtccgac ggcagcaggc
      841 cgtggcggat cagcggggcg aggagggcga ggaacccggt ggggtagcag cccggattgg
      901 agacccggct ggcctcggcg acccgctcgc gcccgaccag ttcgggaaag ccataggtcc
      961 agcccggcgc cacgcggtgc gcgctggagg cgtcgatgat ccgcaccttg ctgccttcgg
     1021 ccagggctac ggcctcgatc gcggcttcgt ccggcaggca cagcacggcg aaatcggcgg
     1081 cgtgataggc cgcgcggcgg gcctcggccg acttgcgctg ctgctcgtcc aggatcacca
     1141 gctcgaactc gctgcgcggg gccaggcgct cgacaatctc gaggccggtg gttccggcag
     1201 cgccatcaat gaaaacagtg tgggtcatct gaaaggcctt agaccaatcc tgcccgtccg
     1261 tcctgagctt gtcgaaggac tgtccttagc cttgcggtca ggggaggtgg gcagggaaga
     1321 aaaagcaacc cttcgacaag ctcagggcga acggaattcg gaggcttacc ctcgtcgctc
     1381 ctgcagcgct tcgaccggca gatagccgac aaacccgtcc tcgccgacct ggccccaggc
     1441 ccagccgccg gtcacgtcca gcacgttgaa caaggtgccg ccgcccagcg tgtcgcgcac
     1501 atcgctgtcc ttgctgttca gcgcgaacag cttcgcgccg cagggcacca cctcgcgctc
     1561 catcggcacg gcataatgcg gcacgaagca gcggcccgcc aggcggatgt gcgccaggtc
     1621 cccgcgcacc ggccaatgcg ccgcatccag cttttcagcc ggaccggaaa gggccagttg
     1681 cccggtcggg atcgcggtgg cggtcaaggt ttcggttccc ccaaactgga agagcgggcg
     1741 attagcgccg ctttcccctt ctagcaagct atccataccg ccccttcagc aggccccagg
     1801 ccgcgcgcag gcccagcgcg tcgccgccct tgggccggcc cggcttggcc gcggggcgcc
     1861 aggcgaaggt atcgaagtgg gcccagtcgg tcccttcggg cacgaaccgg tcgaggaaca
     1921 ggcccgcgac cgaggctccg gcaaagccgt tggagccgga attgttgaga tcggcgatgt
     1981 cgctcttgag gtactcgcgg tagccgtcga acagcggcag gcgccagcag cggtcatcgg
     2041 ccgcctcgcc ggccttcagc agttcatcgg ccgtctcgtc gcgccgggtg aacagcgccg
     2101 g
//
LOCUS       DASUDV010000067         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2078834,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000067 DASUDV010000000
VERSION     DASUDV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078834"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VFV30_01405"
     CDS             complement(<1..988)
                     /locus_tag="VFV30_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX4846774.1"
                     /translation="MTLATSRRGFTAGLAALAVLPSIAEARRSGRYDAIVRGGTVFDG
                     LGGAPFEADVGIAGGRIAAIGKLRQARGALEIDARGQAVAPGFINMLSWANESLIEDG
                     RSQSDIRQGVTLEVMGEGWTMGPLTEEMKRREMRQQDDIRFDIGWTSLAQYLGFLEAK
                     GVSCNVASYVGATTLRMHEVGYDDVRASPDQLRRMQDLVRAEMRGGALGVGSSLIYAP
                     GNFANTDELVALTQAAAEFGGGYISHMRSEAERYFESIDELIEIGRRARARVQMYHIK
                     PAGKDNWDKADAGLARMDAARRAGIDVSANLYTYTAGSTGLYATMPLWVQQGG"
     gene            1151..>2253
                     /locus_tag="VFV30_01410"
     CDS             1151..>2253
                     /locus_tag="VFV30_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4846775.1"
                     /translation="MKTLRSVLLASALLLSPIAAAPALAETAAAPAAANASEHDKLFA
                     LFAASDEARLKRNPLSALFRGDMRYADRLGDFLTPAYNEAERKAASDELAALHAIDRA
                     KLSPTDRIAYDVFEYGRKQTLRGLTPEILALTEVRPINHFFGFHTFYPTLASGKTGIP
                     FKTLTDYQNNLKRHEDYIRLIDRSIAKFREGQKSGVVETKLTIRNVIEQLDTQLKQGV
                     EQSPYWEPVKAMPATFTPAQKKKLTAQYLVTTKRLFAANKRLRDYLANEYLASARDSV
                     GLSQMKGGAALYARQIENTTTLPLTADYLHELGLSEVTRIKGEMTKIMAEVGFKGTMA
                     EFFEFIRTDPQFKRKSREEITQRYYDIGKMVDA"
BASE COUNT          424 a          775 c          681 g          373 t
ORIGIN      
        1 gcccgccctg ctgcacccac agcggcatgg tggcgtaaag accggtcgat ccggcggtgt
       61 aggtatagag gttggccgaa acgtcgatac ccgcccggcg cgccgcatcc attctggcca
      121 ggcccgcatc ggccttgtcc cagttgtcct tgcccgcagg cttgatgtgg tacatctgca
      181 cccgcgcccg ggcgcggcga ccgatctcga tcagttcgtc aatcgattcg aaatagcgtt
      241 ccgcctcgct tcgcatgtgg ctgatatagc cgccgccgaa ttcggccgca gcctgggtca
      301 gcgccaccag ttcatcggta ttggcgaaat tgcccggcgc atagatcagc gacgagccga
      361 cccccagcgc cccgccgcgc atttccgcgc ggaccagatc ctgcatccgg cgcagctgat
      421 cggggctggc acggacgtcg tcatagccaa cctcgtgcat ccgcagcgtg gttgccccga
      481 cataggaggc aacgttgcac gatacgccct tcgcctcgag aaagccgagg tactgggcca
      541 gcgaggtcca gccgatgtcg aagcggatat cgtcctgctg gcgcatttcg cggcgcttca
      601 tctcctcggt cagcgggccc atggtccagc cttcgcccat gacctcgagc gttaccccct
      661 ggcggatgtc gctttgcgag cggccatcct cgatcaggct ttcattggcc caggacagca
      721 tgttgatgaa gccgggggcc acagcctgcc cgcgggcatc gatctccagc gcgccgcgcg
      781 cctggcgcag cttgccgatc gcggcgatcc tgcccccggc gatccccaca tcggcttcga
      841 acggcgcgcc gcccagcccg tcgaacaccg ttcccccgcg cacgatcgca tcgtagcggc
      901 ccgatcgccg ggcctcggcg atggacggca aaacggcaag ggcggcaagg ccggcggtaa
      961 agccgcgcct ggaggtggca agggtcatgg ccgcaagtct tgatccgaac ggcgtggctt
     1021 gcaagggcga acttgcagcg cctggcattg gtggaagccg gcgcgcggtt gatcgaagcg
     1081 gcctgccgcc tcgcctggcg agagcctagg ggcggcgcag ttccacaggt tgtgctttga
     1141 aggatcgccc atgaaaacgc ttcgctccgt cctcctcgcc tcggcgctgt tgctcagccc
     1201 gattgccgcg gccccggccc tggccgaaac tgctgccgcc cctgccgcgg ccaatgccag
     1261 cgagcatgac aagctgttcg cgctgttcgc cgcttcggac gaggcacggc tcaagcgcaa
     1321 cccgctttcc gcgctgtttc gcggcgacat gcgctatgcc gaccggctgg gcgatttcct
     1381 gaccccggcc tacaacgaag ccgaacgcaa ggcggcgagc gatgaactgg ccgcgcttca
     1441 tgcgatcgac cgggccaagc tctcgccgac cgaccggatc gcctacgatg tgttcgaata
     1501 tggccgcaag cagaccctgc gggggctgac cccggaaatc cttgcgctga ccgaagtgcg
     1561 tccgatcaac cacttcttcg ggttccacac cttctacccg accttggcca gcggcaagac
     1621 cggaatcccg ttcaagacgc tgaccgatta ccagaacaac ctcaagcgcc acgaggacta
     1681 catccggctg atcgaccgtt cgatcgccaa gttccgcgaa ggtcagaagt cgggcgtggt
     1741 cgaaaccaaa ctgacgatca ggaacgtgat cgaacagctc gatacccagc tcaagcaggg
     1801 tgtcgaacag tcgccctatt gggaaccggt caaggcgatg cccgccacct tcaccccggc
     1861 gcagaagaag aagctgaccg cgcaatacct ggtcaccacc aaaaggctgt tcgccgccaa
     1921 caagcggctg cgcgattacc tcgccaacga gtatctcgcc tcggcgcgcg acagcgtcgg
     1981 cctttcgcag atgaagggcg gggcggcgct ctatgcccgg cagatcgaga acaccaccac
     2041 cctgccgctg accgccgatt acctgcacga gctgggcctg tcggaagtga cccggatcaa
     2101 gggtgaaatg accaagatca tggccgaggt cggcttcaag ggcaccatgg ccgaattctt
     2161 cgaattcatc cgcaccgatc cgcagttcaa gcgcaagagc cgcgaggaaa tcacccagcg
     2221 ctattacgac atcggcaaaa tggtcgacgc caa
//
LOCUS       DASUDV010000068        12293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_355733,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000068 DASUDV010000000
VERSION     DASUDV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355733"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFV30_01415"
     CDS             complement(<1..765)
                     /locus_tag="VFV30_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4846776.1"
                     /translation="MEPVQKPLYALGLRLLATMVLATLIMVVKYTADSGVAFPEILFW
                     RQVPSAVLILAWLAMRGQVHRLKTKRLPEHGRRAMLGMTGMFFTFGAPVLLPLAVATT
                     LGFTTPVFAVILSALLLKERVGPVRWLAVALGFAGVVIIAQPGHTAIPALGAFVGLGA
                     GFMVALISIQVRDLGRTEEPLSIVFWFAALSAPVLALGLPFYAAAHTPWQWFLLLGGG
                     VLGLIGQLLLTASLRYGQVASVIVMDYSALIWATLYG"
     gene            788..1015
                     /locus_tag="VFV30_01420"
     CDS             788..1015
                     /locus_tag="VFV30_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846777.1"
                     /translation="MDWQATIADWLDTAWVRSSLLAFALACLAVVAWRGDRRRMTRSD
                     ADAVGWMPWRDIAFWSAFAAFLVAVFGVYFV"
     gene            complement(1026..2555)
                     /locus_tag="VFV30_01425"
     CDS             complement(1026..2555)
                     /locus_tag="VFV30_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX4846778.1"
                     /translation="MSAFGRRNGVGGMAPGARPQFGVARPMKGGIDAAKSPGAPGGDQ
                     FPPLPGEEAVPLPPLDGGPATKADAMTRLQDRINGVSEGNYQAEGFEASVHKIKEQVL
                     PRLLERVDPEAAASLSKDELSEEFRPIIMEVLAELKVTLNRREQFALEKVLIDELLGF
                     GPLEELLNDPDVSDIMVNGPMQTYIEKKGKLQIAPIQFRDEQHLFQIAQRIVNQVGRR
                     VDQTTPLADARLKDGSRVNVIVPPLSLRGTAISIRKFSEKPITIDMLRDFGSMSDKMA
                     TALKIAGACRMNIVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLP
                     LETRPPNLEGQGAITIGDLVKNALRMRPDRIILGEIRGAECFDLLAAMNTGHDGSMCT
                     LHANSPRECLGRMENMILMGDIKIPKEAISRQIAESVDLIVQVKRLRDGSRRTTEITE
                     VIGMEGDVIVTQSLFKFEYLDESEDGKIIGEFRASGLRPYTLEKARQFGFDQAYLEAC
                     L"
     gene            complement(2655..3743)
                     /locus_tag="VFV30_01430"
     CDS             complement(2655..3743)
                     /locus_tag="VFV30_01430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4846779.1"
                     /translation="MDTPAPDILFITRKWAPAVGGMETWSHRLSDSLSERAAVEVVAL
                     PGRPGGLPPTVPALLAFPLIALRRYLARDAAPDVLHIGDMALWPLGLLACLRRGSPAI
                     VLTAHGTDVSYARRPGWRGRAYRAYQRLGARLLRRAHVTANSPATAAAAAEAGWRQVT
                     VIPLATDLTGHVPDQPPQPHILFAGRLIPLKGCGWFIRSVLPLLPDGVTLKVAGTLLD
                     PAEAAALDHPRVEYLGVLDQAALSAEYPRAACVVVPNIVPSNRTFEGFGLVAVEAAAA
                     GGVTLAANCGGIPAAVIDGTTGILVESGDAAAWAEAIGAVLAWPLPRREAFLAGSLAA
                     VESAFRWPRVAEATLAVYEQTRNDPSGL"
     gene            3834..4526
                     /locus_tag="VFV30_01435"
     CDS             3834..4526
                     /locus_tag="VFV30_01435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4846780.1"
                     /translation="MIKSLVKRTPVVRDIARAVLKLLVDRRQKDFVSGDYWENRYKAG
                     GNSGAGSYNRLAEHKAEVLNGFVADHGVQTVIEFGSGDGAQLTRAHYPDYTGVDISHT
                     ALDLTRKLFAGDPTKRFLHPSELPDPFVADLTLSLDVVYHLIEDSVFEAYMAQLFDAS
                     RRYVIVYSSNTDRKSDAVHVRHRRFTDWVEANRPEFALTGVVRNPYPEDQDTDNTSFA
                     DFYFFEKPAAAG"
     gene            complement(4493..5446)
                     /locus_tag="VFV30_01440"
     CDS             complement(4493..5446)
                     /locus_tag="VFV30_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX4846781.1"
                     /translation="MTAPSSVPAPQGWLKRHQRVLLRAAALVFFLASLAYVGLFAQRN
                     WAAVRTLPPLDGSAAALAAALYLLAHPFTASAWILGLRGLGHDLPYQTGLRISLAAQI
                     GKYLPGNVAHYFARAGMAAGAGIALSGSGLATVLEIVSALLAAALVAAAAMLLDPAPA
                     AVLHQAFAAQPALPLLLAAGATIGTAVIVRLTALPLRVLAVMTLCMTANFLLVGLSFH
                     AVVAALAGPAPSVAAMLGVYTVAWTVGYLVPGAPAGLGVREAVLVAWLSAIIGVGPAL
                     ACTVLHRLITAAVDTAVGLAGYAWHRAARSDQPAAAGFSKK"
     gene            complement(5449..7125)
                     /locus_tag="VFV30_01445"
     CDS             complement(5449..7125)
                     /locus_tag="VFV30_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846782.1"
                     /translation="MIGIRESRIRLPQLSPDAARHERRFVLAAFLAILLLQLEQVFNR
                     PVNWDEFYHLSQVHAQAQGRLSEVLQVLHVRLLGWLALLPLGEIDQVRIARLAMFGCE
                     LFTLLAIHGLARRLVQERAARLAPLLYLGAGFVFQHGMAFRADPLVTALLMGALWWLA
                     AGALRGRDCAGIAVLLALAGLATVKAVLFAPAFAAIAWWRLTQQADPKAFALRLAATV
                     LLSGALLAVLVLITQQFVPPAPAVSAAATVQASQSMLLAEGLLPRWQFLLMAAATAPM
                     LVLAGAAMVPGLWRTAVPGTLRIVCIGLILPLASVLFYRNAFPYFYAFILAPVVVGLV
                     PGIAWLARSGGVRAMAAGSLLGCVLLSAITPRAVLGEQGAVLREVHRIFPQPVAYFDF
                     AGMVAAFPKANVFMSSWGQTRYRSGEFASYADTVQQRDVPLLLANHDVLARNQSPGGP
                     AWELLPHDAVLLRTSYVQHWGPIWLAGRRFASGAPEQDFTLPAGGTYTLSGTAALIDG
                     QRIEPGSTVALARGRHRFAPLGPGETVLRWGKDLPRPSGPAPAGVPFRGF"
     gene            complement(7122..8117)
                     /locus_tag="VFV30_01450"
     CDS             complement(7122..8117)
                     /locus_tag="VFV30_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4846783.1"
                     /translation="MTKLIIQIPCFNEAESLPETLVALPRRLPGIDTVEILVIDDGSH
                     DGTAEVARRFGVHHVVRHRRNRGLAAAFQTGIDAALAAGADIIVNTDADGQYAGEDIA
                     KLVAPIVAQEADLVIGDRGVGENAHFGPFKRKLQRLGSAVVRSLSRTEVTDAVSGFRA
                     LSRAAAQRITITTEFSYTTDMLIQAGRKRFKIVSVSVRTNATERPSRLFKSIPQFIAR
                     QLMTMARAYATYNPLRTFALIGGTIALVGTVPIVRFLWFWANGAGNGHVQSLVLGGSL
                     LVLGVLTGLMGLLAELIGANRKLLEATLSHVRKLEDRLAQLEPGTPTETDRKRRA"
     gene            8248..9147
                     /locus_tag="VFV30_01455"
     CDS             8248..9147
                     /locus_tag="VFV30_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4846784.1"
                     /translation="MRQNERAGLLYALAGFATLSFGDAVIKTMAGMWSPVAIAALRFS
                     LAAVGLAALLGWREGRAGFQLAQPWAQFGRGAALAVSSTVFFTSLFVLPLATATAIGF
                     TTPMFTAILAAVLLGEPLRRATLIASAVAFAGVLVVLRPNLAEAGLWAVLPIFSAITF
                     SLLIIGNRHVAGTASALAMQFWVATFAAPVLLLAAPLFAQTGVERFALGWPDWTVVAR
                     CAFVAVSATCAHWLIYLGTTRAGAAVVAPATYIQLLVATFLGWLWFDTHPDAMTLLGA
                     GIIIGSGLYLWREGQVREPPESE"
     gene            9144..9425
                     /locus_tag="VFV30_01460"
     CDS             9144..9425
                     /locus_tag="VFV30_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX4846785.1"
                     /translation="MSGEGPDPCWLCRRPLGRRTEWHHPVPKGKGGRAVVPLHPICHR
                     TIHAHFTNAELARHGGAREVLLGSPAMVRFLDWIADKPPDFHARTARPR"
     gene            9492..9965
                     /locus_tag="VFV30_01465"
     CDS             9492..9965
                     /locus_tag="VFV30_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX4846786.1"
                     /translation="MKKMLAAALLLVAAPVLAQSVDKPELPGWMAGTWMMEDGANWSD
                     EVWTDPRGGIMLGVSRSGFGPELQFWEVAQIRVKPDGRISLFAQPQGKAASEFPMVLI
                     SEESIEFANPAHDYPQRIRYWRQGKLLMAEISLMDGSRAQRWNFRPVVPPRDEVE"
     gene            9962..10804
                     /locus_tag="VFV30_01470"
     CDS             9962..10804
                     /locus_tag="VFV30_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX4846787.1"
                     /translation="MMRSRPFKQVDVFTAVPYKGNPVAVVLDGEGLSDAEMQAFANWT
                     NLSETTFVLPPSDPAADYRVRIFTPKAELPFAGHPTLGTAHAVLEAGLASARDGRLVQ
                     ECAVGLVDLTVGEQGLSFKLPRYALEELTDPEALAWLGEGVSVKGPAQAVNVGPTWLV
                     AELAEVAALENLQHDPARLTRFYEATGMTGATVFAVDGDRVVVRSFAPGDGIPEDPVC
                     GSGNGAVAAYRLLAGQVGDGDSYVASQGRQVGRDGYISIRFEGRDIHVGGQAVTCVEG
                     AVSV"
     gene            10833..11156
                     /locus_tag="VFV30_01475"
     CDS             10833..11156
                     /locus_tag="VFV30_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX4846788.1"
                     /translation="MNAWIYLGFAIALEIAGTFLLKFSNGFEKWHWGAASIACYSACF
                     WVLAPAMKVLPLGVVYAIWSGVGIVAAALIGVFAFDERLGAIQYVFIAMVLIGAVGLN
                     LSTAS"
     gene            complement(11214..11471)
                     /locus_tag="VFV30_01480"
     CDS             complement(11214..11471)
                     /locus_tag="VFV30_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX4846789.1"
                     /translation="MPLHFELVTPARLVRSDDVYMVVVPGTEGEFGVLEGHAPFMSTV
                     RDGTLKIYKTDGGQPEEIQVQGGFAEVGENGLTVLAEHVEG"
     gene            complement(11479..11991)
                     /locus_tag="VFV30_01485"
     CDS             complement(11479..11991)
                     /locus_tag="VFV30_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846790.1"
                     /translation="MRIGAAVAVAVAALAAAAPAAAQDRKAELQQLAFETCPKVLDGS
                     LSLDNPADLATLGFASTEPRETPAGKLPRAAKGSGTDKVVLSSGPKTCSLWFGGPDNA
                     SLAGGMIERALALKWSGSTTPRRLGDGTMVFVFRDKVAKRSVVVILADAGGELDFAPA
                     TTVIVMNEKD"
     gene            complement(11991..>12293)
                     /locus_tag="VFV30_01490"
     CDS             complement(11991..>12293)
                     /locus_tag="VFV30_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX4846791.1"
                     /translation="LQKYKSLQDIIAILGMDELSEEDKLTVARARKIQRFLSQPFHVA
                     EVFTNIPGKFVQVEDTVRSFKAVVDGEYDHLPEAAFYMVGGIDEAVEKAKKLAEEA"
BASE COUNT         2015 a         4068 c         4253 g         1957 t
ORIGIN      
        1 gccgtagagc gtcgcccaga tcagcgcgga atagtccatc acgatcacgc tggcgacctg
       61 gccatagcgc agcgaggcgg tgagcagcag ctgcccgatc agacccagca cgccgccgcc
      121 cagcagcagg aaccactgcc agggcgtgtg agctgcggca tagaacggca ggcccagcgc
      181 cagcaccggc gcggaaagcg cggcaaacca gaacacgatc gacagcggct cctcggtccg
      241 gcccagatcg cgcacctgga tgctgatcag cgccaccatg aagccggcgc ccaggccgac
      301 aaaggccccg agggcgggaa tcgcggtgtg gccgggctgg gcgatgatca ccacgccggc
      361 aaagcccagc gcgacggcca gccaacgcac cgggccgacc cgttccttca gcagcagggc
      421 cgagaggatc accgcgaaga ccggggtggt aaagcccagc gtggtggcca ccgccaacgg
      481 cagcagcacc ggtgcgccaa aggtgaagaa catgcccgtc atgcccagca tggcccgccg
      541 gccgtgttcg ggcaggcgct tggttttcag gcggtgaacc tgcccgcgca tggcgagcca
      601 ggccaggatc agcacggccg agggcacttg ccgccagaac aggatctccg gaaaggcgac
      661 gccgctgtcc gcggtgtatt tcaccaccat gatcagggtg gcgagcacca tggtcgccag
      721 cagccgcagg cccagcgcgt aaaggggctt ctggacaggt tccaccgctt cgctctaacg
      781 gcagggaatg gactggcaag caacgatcgc cgattggctg gacaccgcat gggtccgctc
      841 cagcctgctc gcctttgcgc tggcgtgcct ggcggtggtc gcctggcggg gcgatcggcg
      901 ccggatgacc cgcagcgatg ccgatgccgt gggctggatg ccctggcgcg acattgcctt
      961 ctggtcggcc ttcgcggcct tcctggtggc ggtgttcggc gtctacttcg tctagccggc
     1021 aggccttaga ggcaggcttc cagataggcc tgatcgaagc cgaactggcg ggccttctcc
     1081 agcgtgtacg ggcgcaggcc cgacgcgcgg aattcaccga tgatcttccc gtcctcgctc
     1141 tcgtcgagat actcgaactt gaacagcgac tgggtgacga tcacgtcgcc ttccatcccg
     1201 atcacctcgg tgatctcggt ggtgcggcgc gaaccgtcgc gcaggcgctt cacctgcacg
     1261 atcaggtcga ccgattcggc gatctggcgc gaaatggctt ccttcgggat cttgatgtcg
     1321 cccatcagga tcatgttttc catacggccc aggcactcgc gcgggctgtt ggcgtgaagc
     1381 gtacacatcg aaccgtcgtg gccggtgttc atggcggcga gaaggtcgaa acactccgcg
     1441 ccgcggattt cgcccaggat gatccggtcc ggacgcatac gcagggcgtt cttgacgagg
     1501 tcaccgatgg tgatcgcgcc ctggccttcg aggttcggcg ggcgggtttc cagcggcagc
     1561 cagtgcggct gctgcaggcg aagttcggcg gcgtcttcga tggtcagcac gcgctcgccc
     1621 gggtcgatca tcttcgacag ggcgttgagc atggttgtct tacccgaacc ggtaccgccc
     1681 gagatgacga tgttcatccg gcaggcgccc gcgatcttca gcgcggtggc catcttgtcc
     1741 gacatcgagc cgaaatcgcg cagcatgtcg atcgtgatcg gcttctcgga gaacttacga
     1801 atcgagatcg cggtgccgcg caggctgagc ggcggcacga tcacgttgac gcggctgccg
     1861 tccttgaggc gggcgtcggc cagcggcgtg gtctggtcaa cgcggcggcc gacctggttc
     1921 acgatgcgct gggcgatctg gaacaggtgc tgttcgtcgc ggaactggat cggcgcgatc
     1981 tgcagcttgc ccttcttttc gatgtaggtc tgcatcggcc cgttgaccat gatatcggac
     2041 acgtccgggt cgttcagcag ctcttccagc ggaccgaagc ccagcagttc gtcgatcagg
     2101 accttttcca gcgcgaactg ctcgcgccgg ttcagcgtga ccttcagttc ggccagcact
     2161 tccatgatga tcggacggaa ttcttcggac agctcgtcct tcgacaggct ggcggcggct
     2221 tcggggtcga cgcgctccag caggcgggga aggacctgtt ccttgatctt gtgaaccgag
     2281 gcttcaaagc cttcggcctg gtaattgcct tcggacacgc cgttgatccg gtcttgcaac
     2341 cgggtcatcg cgtcggcctt ggtggcgggg cctccgtcca gcggcggcag cggaacagct
     2401 tcctcgcccg gcaggggcgg gaactgatcg ccgcccggcg cacccggcga cttggcggca
     2461 tcgatcccgc ccttcatcgg acgggcgaca ccgaattgcg gcctggcccc gggggccatg
     2521 cccccaacac cgttgcgtcg gccaaatgcg ctcatcgtgc ctctatcccc cggattctgc
     2581 agtcacattg cccgccgcat ggataccgac gggctaatcg aaatggtgaa taacccggaa
     2641 accttgaata ttttctaaag cccggaggga tcgttgcggg tttgctcata tacggcgagc
     2701 gtggcttcgg ccacgcgggg ccagcggaac gcgctctcca ccgccgccag cgagccggca
     2761 aggaaggcct cgcgccgggg caggggccag gccaggaccg cgccgatcgc ctcggcccag
     2821 gccgccgcat ccccgctttc caccaggatg ccggtggtgc cgtcaatcac cgcggcggga
     2881 atccccccgc aattggccgc cagcgtgacc ccgccggccg cggccgcttc caccgcgacc
     2941 agcccgaagc cttcgaaagt gcggttggaa ggaacgatgt tgggcaccac cacacaggcc
     3001 gcgcgcgggt attccgcgct gagggccgcc tgatccagca cgccaaggta ttcgacccgg
     3061 gggtggtcca gcgcggcggc ttcggcggga tcgagcaatg tgccggcgac tttcagggtt
     3121 acgccgtcgg gcagcagcgg caggaccgaa cggatgaacc agccgcaccc tttcagcggg
     3181 atcagccgcc cggcgaacag gatgtgcggc tgtggcggct ggtccggcac atggccggtc
     3241 agatcggtcg ccagcgggat caccgtcacc tggcgccagc ccgcctcggc cgcggcggcg
     3301 gcggtggcag gggaattggc ggtcacatgg gcgcggcgca gcagccgggc tcccagccgc
     3361 tgataggccc ggtaagcccg cccgcgccaa ccgggacggc gggcatagga cacatcggtg
     3421 ccgtgggcgg tcagcacgat cgccggactt ccgcggcgca ggcaggcgag caggcccagc
     3481 ggccacagcg ccatgtcgcc gatatgcagc acgtcgggcg cggcatcccg ggccagatag
     3541 cggcgcagcg caatgagggg aaaggccagc agcgccggga ctgtgggggg caggccccct
     3601 ggccggccgg gcagggccac aacctcaacc gcggcccgct ccgacaggct gtcgctcagc
     3661 cggtgcgacc aggtttccat gccgcccacg gccggagccc acttgcgggt gatgaacagg
     3721 atgtcgggcg ctggcgtgtc caaaatgcgg gtgcttccgt ttcggttgtg cgcggcatcc
     3781 tgcccggtgc ctcgtcgctt gtcttcaatg ctatttcacc ctagtcccgc gcaatgataa
     3841 aaagcctggt aaagcgcacc ccggttgtcc gcgatatcgc gcgcgcggtg ctcaagcttc
     3901 tggtcgatcg gcgccagaag gatttcgttt cgggcgacta ttgggagaac cgctacaagg
     3961 cgggcggcaa ctcgggcgcg ggatcctaca accgtctggc cgagcacaag gcggaggtcc
     4021 tgaacggctt tgtcgcggac cacggcgtgc agaccgtgat cgaattcgga tcgggcgacg
     4081 gcgcccagct gacccgggcg cattatcccg actacaccgg ggtggatatc tcgcacaccg
     4141 cgctggacct gacccgcaag ctgttcgctg gcgatccgac caagcgcttc ctccatccca
     4201 gcgagttgcc cgatcccttc gtggcggacc tgacgctgtc gctcgacgta gtctatcacc
     4261 tgatcgaaga cagcgtgttc gaagcctata tggcgcagct gttcgatgcc tcgcggcgct
     4321 atgtgattgt ctattccagc aacaccgacc gcaaatccga tgcggtccat gtccgccacc
     4381 ggcgctttac cgactgggtg gaggccaacc ggccggaatt cgcgctgacc ggggtggtgc
     4441 gcaatcccta tcccgaggat caggataccg acaacacctc gttcgcggac ttctatttct
     4501 tcgaaaagcc cgctgctgcg ggctgatcgc tgcgggccgc gcggtgccag gcgtaaccgg
     4561 ccaggccaac cgccgtatcg accgctgcgg tgatcagccg gtgcagcacc gtgcaggcca
     4621 gggccgggcc aaccccgatg atcgcgctga gccaggcgac cagcaccgct tcgcgcacgc
     4681 caagcccggc cggggcgccc ggcaccagat agccgaccgt ccaggccacg gtgtagaccc
     4741 ccagcatggc cgcgaccgat ggcgcgggtc ccgcgagcgc ggcgacaacc gcgtggaagg
     4801 acaggccgac cagcaggaag ttggccgtca tgcacagcgt catcaccgcc agaacccgca
     4861 gtggcagggc ggtgagccgc acaatcacgg cggtgccgat cgtggcgccg gctgccagca
     4921 gcagcggcag ggccggctgg gcggcaaagg cctgatgcag gaccgcggcc ggggccggat
     4981 cgagcagcat ggccgcggcg gcgaccagcg cggcggcgag cagggcggac acgatttcca
     5041 gcacggtggc aaggccgctg ccgctgagcg caattcccgc cccggcggcc atgccggccc
     5101 gcgcgaaata gtgcgcgacg ttgccgggca ggtacttgcc gatctgggcg gccagcgaaa
     5161 tcctgagccc ggtctggtac ggcagatcgt ggccaaggcc ccgcagcccg aggatccagg
     5221 ccgaagcggt gaagggatgg gccagcagat agagcgcggc ggccagcgcg gcggcagagc
     5281 cgtccagcgg gggcagggtg cggacggcgg cccagttgcg ctgggcgaac aggccgacat
     5341 aggccaggct cgccaggaaa aagaccagcg ccgccgcccg cagcaggacg cgctgatgcc
     5401 gcttgagcca gccttgcgga gccgggacag aggaaggggc ggtcacgctc agaacccccg
     5461 gaacggcacg ccagcggggg cggggccgga cgggcggggc aggtccttgc cccagcgcag
     5521 cacggtttca ccgggcccca gcggagcaaa gcggtggcgg ccgcgcgcca gcgcgacggt
     5581 gctgccaggc tcgatccgct gcccgtcgat cagggcggcc gtgccggaca gggtgtaggt
     5641 cccgccagca ggaagggtga agtcctgttc gggcgcaccg gaagcaaaac gccggccggc
     5701 aagccagatc gggccccagt gctggacata gctggtccgc agcaggacgg cgtcgtgggg
     5761 cagcagctcc caggccgggc ctcccggcga ctggttgcgg gccagcacat cgtgattggc
     5821 cagcagcaag ggcacgtcgc gttgctgcac cgtatcggca tagctggcga attcgccgct
     5881 ccggtaccgg gtctggcccc acgagctcat gaagacattg gctttgggga aagcggcgac
     5941 catgccggcg aaatcgaaat aggccaccgg ctggggaaag atgcggtgca cctcgcgcag
     6001 cacggcgccc tgttcgccca gcacggcgcg cggggtgata gccgagagca ggacgcagcc
     6061 cagcaggctg cccgctgcca tcgcgcgaac cccgccgctc cgggccagcc aggcaatccc
     6121 ggggaccagc ccgaccacga ccggagccag gatgaaggcg tagaaatagg gaaaggcgtt
     6181 gcgatagaag aggacgctcg ccagcggcag gatcaggcca atgcagacga tccgcagcgt
     6241 tccgggcacg gccgtgcgcc acaggcccgg caccatcgcg gcaccggcca gcaccagcat
     6301 cggcgcggtc gcggcggcca tcagcaggaa ctgccagcgc ggcagcagcc cttcggccag
     6361 cagcatcgac tgcgaggcct gaaccgtcgc cgcggcgctg accgccgggg cgggcgggac
     6421 gaactgctgg gtgatgagta cgagtacggc cagcagcgct ccggacagca ggacggtggc
     6481 ggccaggcgc agggcaaagg ccttgggatc ggcctgctgg gtcagccgcc accaggcgat
     6541 cgcggcgaag gccggggcga acagcacggc cttgaccgtg gccagcccgg ccagcgccag
     6601 cagcacggcg atcccggcgc aatcgcgtcc gcgcagcgcc cctgccgcca accaccacag
     6661 cgcgcccatc agcagcgcgg tgaccagcgg atcggcgcga aaggccatgc cgtgctgaaa
     6721 gacaaagccc gcgcccaggt aaagcagcgg ggccagccgc gcggcgcgtt cctgcaccag
     6781 ccgccgggcc aggccgtgga tggcgagcaa ggtgaacagc tcacagccga acatcgccag
     6841 ccgggcaatc cggacctggt cgatctcgcc cagcggcagc agcgccagcc agccgagcag
     6901 gcggacgtgg agcacttgca gcacttcgct cagccggccc tgggcctggg cgtgaacctg
     6961 gctgaggtgg tagaattcgt cccagttgac cgggcggttg aagacctgtt ccagctgcag
     7021 cagcagaatg gccaggaagg cggccagcac gaagcggcgc tcgtgccggg ccgcatcggg
     7081 ggaaagctgc ggcaggcgga tccgggattc ccggatcccg atcatgcgcg gcgctttctg
     7141 tccgtctcgg tcggggtgcc cggttccagc tgcgccagcc ggtcttccag cttgcggaca
     7201 tggctgagcg tggcctccag cagcttgcgg ttggcgccga tcagttcggc gagcaggccc
     7261 atcagtccgg tcagcacccc caggaccagc agcgagccgc ccagcaccag tgactggaca
     7321 tggccgttgc cggcgccgtt cgcccagaac cacaggaacc ggacgatcgg cacggtgccg
     7381 accagcgcga tcgtgccgcc gatcagcgca aaggtgcgca gcgggttata ggtcgcatag
     7441 gcccgggcca tggtcatcag ctggcgggcg atgaactgcg ggatcgactt gaacagccgg
     7501 ctcggccgct cggtggcgtt ggtccgcacc gacacgctga cgatcttgaa ccgcttgcgc
     7561 ccggcctgaa tcagcatgtc ggtggtgtag gaaaattcgg tggtgatcgt gatccgctgg
     7621 gcggcggcac gcgacagcgc gcggaaaccg ctcacggcat cggtcacttc ggtgcgcgac
     7681 aggctgcgca ccacggcgct gcccagccgc tgcagcttgc gcttgaacgg gccgaaatgg
     7741 gcgttttcgc ccactccgcg gtcgccgatg accagatcgg cctcttgcgc gacgatcggg
     7801 gcgaccagct tggcaatgtc ctcgccggcg tattggccgt cggcgtcggt gttgacgatg
     7861 atgtcggccc cggcggccag cgcggcatcg atgccggtct ggaacgcggc ggccaggccc
     7921 cggttgcggc ggtggcggac cacgtgatgg acgccgaacc ggcgcgcgac ctcggccgtg
     7981 ccgtcgtggc tgccgtcatc gatgacaagg atttcgaccg tgtcgatccc gggcaggcgc
     8041 cggggcaggg cgacgagcgt ctccggcagg ctctctgcct cgttgaagca cgggatctgg
     8101 atgatcagct ttgtcacatg ggccccggtt gcgcgtgcgt ggttaggggt tgttaagcat
     8161 gactgtttaa cagcgggtaa accttgccgc ccccttggcg cgcgccccct cgctgcctag
     8221 aggcgtgagc cgggaggact tttcaccgtg cgccagaacg aacgggccgg actgctctat
     8281 gcgctggcgg gctttgccac gctgtcgttc ggcgatgcgg tgatcaagac catggccggg
     8341 atgtggtcgc cggtagccat tgctgcactg cgtttcagcc tggcggcagt gggcctcgcc
     8401 gcgctgctgg gctggcgcga agggcgcgcc gggttccagc tggcccagcc ctgggcgcag
     8461 ttcgggcggg gcgcggcgct ggcggtgtcc tccaccgtgt tcttcacctc gctgttcgtg
     8521 ctgcccttgg cgacagccac cgcgatcggc ttcaccaccc cgatgttcac cgcgatcctc
     8581 gccgcggtcc tgctgggcga gccgctgcgc cgggcgactc ttatcgcctc ggcggtggcc
     8641 tttgccgggg tgctggtcgt gctccgcccg aacctggccg aagccgggtt gtgggcggtg
     8701 ctgccgatct tctccgcgat cactttcagc ctgctgatca tcggtaaccg ccatgtcgcc
     8761 ggcaccgcat cggcgctggc catgcagttc tgggtcgcca cctttgccgc cccggtcctg
     8821 ctgctggccg cgccgctgtt cgcccagacc ggggtggagc ggttcgcgct gggctggccg
     8881 gactggaccg tggtggcgcg ctgcgcgttt gtcgcggttt cggccacctg cgcgcactgg
     8941 ctgatctacc tcggcacgac ccgcgccggc gcggcggtgg tggccccggc cacctacatc
     9001 cagctgctgg tggcgacctt cctgggctgg ctgtggttcg atacccaccc cgatgcgatg
     9061 accctgctgg gcgcaggtat catcatcggc tcgggccttt atctgtggcg cgaagggcaa
     9121 gtgcgcgagc cgccggagag cgaatgagcg gggaggggcc ggacccgtgc tggctgtgcc
     9181 gccgcccgct gggcaggcgg accgaatggc accacccggt gcccaagggc aagggcggcc
     9241 gggcggtggt gccgctgcac ccgatctgcc accgcacgat ccacgcccat ttcaccaacg
     9301 ccgaactggc ccgccacggc ggcgcgcgcg aggttctgct ggggagcccg gccatggttc
     9361 ggttcctgga ttggatcgcg gacaagccgc ccgatttcca tgcccgcacg gcccgtccgc
     9421 gctaggcttg gccgcgcggc gccaagccgc taaacccgcc gttcatttcg ccccgggcag
     9481 aggttccccc gatgaagaag atgctcgctg cagccctgct gcttgttgcc gcgccggtgc
     9541 tggcgcagtc ggtcgacaag cccgaattgc cgggctggat ggccggcacc tggatgatgg
     9601 aagacggggc caactggtcg gacgaggtgt ggaccgatcc gcgcggcggg atcatgctgg
     9661 gcgtctcgcg ctcgggcttc gggccggaac tgcaattctg ggaagtggcg cagatccggg
     9721 tcaagccgga cggccggatc agcctgttcg cccagccgca aggcaaggcg gccagcgaat
     9781 tcccgatggt gctgatcagc gaggaatcga tcgaattcgc caatccggcg cacgattacc
     9841 cgcagcggat ccgctattgg cggcagggca agctgctgat ggcggaaatc tcgctgatgg
     9901 acggcagccg cgcccagcgc tggaatttcc ggccggtggt gccgccaagg gatgaggtgg
     9961 aatgatgcgc agccgtccgt tcaagcaggt cgatgtcttc accgccgtgc cctacaaggg
    10021 caacccggtg gccgtggtgc tcgacgggga agggctctcc gatgcggaga tgcaggcctt
    10081 cgccaactgg accaaccttt ccgagacgac cttcgtcctg ccgccgagcg atcccgcagc
    10141 ggattaccgg gtgcggatat tcacgcccaa ggccgaactg ccgtttgccg gtcacccgac
    10201 gctgggcacc gcccacgccg tgctggaagc cgggctggca agcgccaggg acggccggct
    10261 ggtgcaggaa tgcgcggtcg gtctggtcga tctgaccgtg ggtgagcagg gcctgtcgtt
    10321 caagctgccg cgctatgcgc tggaagagct gaccgatccc gaggcgctgg cctggctggg
    10381 cgagggcgtt tccgtcaagg ggccggccca ggcggtcaat gtcgggccga cctggctggt
    10441 cgccgaactg gccgaggtgg cggcgctgga gaacctgcag cacgaccccg cccggctgac
    10501 gcgcttttac gaagccaccg gcatgaccgg cgcgaccgtc ttcgcggtcg atggggaccg
    10561 cgtcgtggtc cgctccttcg cccccggcga tggcatcccg gaagacccgg tttgcggcag
    10621 cggcaacggc gcggtggctg cctaccggct gctggccggg caggtcgggg atggggacag
    10681 ctatgtcgcc agccagggcc gccaggtcgg gcgggacggg tatatctcga tccgcttcga
    10741 agggcgcgac atccatgtcg gcggacaggc ggtgacctgt gtagagggcg ccgtgagcgt
    10801 ctaggccggc cgttacgaaa ggaaagcggg cgatgaatgc ctggatctat cttggctttg
    10861 ccattgccct tgagattgcc ggaaccttcc tgctcaagtt ctccaacggg tttgagaagt
    10921 ggcactgggg cgccgcgtcg atcgcctgct actcggcgtg tttctgggtg cttgccccgg
    10981 caatgaaggt gctgccgctg ggcgttgtct atgcgatctg gtccggcgtg gggattgtcg
    11041 ctgcggcgct gatcggtgtc ttcgccttcg atgagcggct gggcgcgatc cagtacgtct
    11101 tcatcgccat ggtcctgatc ggcgcggttg gcctgaacct ctccaccgcc agctgaatcg
    11161 aaaagggcgg cccaagcgga ccgccctttt gcattcggta tcggccgaaa gcctcagcct
    11221 tcgacgtgtt ccgccagaac ggtcaggccg ttttcgccga cttcggcaaa gccgccctgg
    11281 acctggattt cctccggctg gccgccatcg gtcttgtaga tcttcagggt gccgtcgcgg
    11341 acggtcgaca tgaaaggcgc gtggccttcc agcacgccga actcgccctc ggtgccgggg
    11401 acgacgacca tgtagacgtc gtccgaccga accagacgcg caggcgtgac gagttcaaag
    11461 tgaagaggca tgagctaact agtccttttc attcatcacg atgaccgtgg tcgcgggggc
    11521 gaaatcaagt tcaccacctg catcggccag gatgacaacc accgatcgct ttgcgacctt
    11581 gtcgcgaaat acaaacacca tcgttccgtc gccaagtcgc cgcggggtcg tgcttcccga
    11641 ccacttcagc gccagcgccc gttcgatcat ccctccggcc agtgacgcat tgtctggtcc
    11701 accgaaccac aagctgcagg tcttcggacc tgaggacagc acaaccttgt ccgtcccgct
    11761 tccctttgca gcacgcggca gcttgcctgc cggagtttcg cgcggctcgg ttgaagcgaa
    11821 gcccaacgtg gccagatcag ccgggttgtc gagcgaaagg ctgccatcaa gaaccttggg
    11881 gcaggtttca aacgccagct gctgcaactc ggccttgcga tcctgggcag cagcgggggc
    11941 agcagcggca agtgccgcca ccgcaaccgc cacggctgcc ccaatgcgca ttaggcttcc
    12001 tcggccagct tcttggcctt ttcgaccgct tcgtcgatgc cgccgaccat gtagaaagca
    12061 gcttcgggca ggtggtcgta ctcgccgtcc acaaccgcct tgaacgaacg cacggtgtct
    12121 tcgacctgga cgaacttgcc cgggatgttg gtgaagactt cggcgacgtg gaacggctgc
    12181 gacaggaagc gctggatctt gcgggcgcgg gcgacggtga gcttatcttc ttcgctcagt
    12241 tcgtccatgc cgaggatcgc gatgatgtcc tgcagcgact tgtacttctg cag
//
LOCUS       DASUDV010000069         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_289427,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000069 DASUDV010000000
VERSION     DASUDV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289427"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFV30_01495"
     CDS             <1..873
                     /locus_tag="VFV30_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX4846792.1"
                     /translation="IAIIIASSQLKDFFGFSGAALPAEFIPKLAALWAVRGTFNATAL
                     LIGIAALILIVLLRRLFPRFPGLIVAMTVVSAAVALGHLPVDTIASRYGALPGHLPMP
                     AVPAVSLERLGDLLPSALVIAFLAAIESLLSAMVADRMIEGSHRPNAEVIAQGWANIA
                     SALFGGLPATGAIARTATNIRAGATTPVAGLVHAIVILLVLLVAAPLAGFLAMPALAA
                     LLLTTAWNMSEPHKWRSYWAAPAGDRLLLLITLVLTVLSDLTVAIGVGVALGLALRLR
                     DRQLEADRWRTPER"
     gene            complement(870..1154)
                     /locus_tag="VFV30_01500"
     CDS             complement(870..1154)
                     /locus_tag="VFV30_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX4846793.1"
                     /translation="MARPRADLPDPQAILAQVDPEGRLALRVTPGARTESIAIEGGQV
                     LIKVRARPEDGKANAAVLDLLAKALDLAPSRLTLLRGATGRDKLVRIDRS"
     gene            complement(1135..1707)
                     /locus_tag="VFV30_01505"
     CDS             complement(1135..1707)
                     /locus_tag="VFV30_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4846794.1"
                     /translation="MTTPRPDTTILGIHGKSPRIHDSAFIAPGCRIIGDVEIGPDASI
                     WYNCVIRGDASRVVIGARTNVQDGSVIHCDAAMPGAPDGYPTLIGDDVLIGHMAMIHG
                     TVIEDRGFVGFAAQTMNGCVIEADGMLAAGALLAPGKRIPARQLWSGRPATFMRELTD
                     AALAGLQMGVKHYVENARAHQALYDGSAQG"
     gene            complement(1707..>3136)
                     /locus_tag="VFV30_01510"
     CDS             complement(1707..>3136)
                     /locus_tag="VFV30_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846795.1"
                     /translation="FAEGRRPARIIAALTWPVLLGAGGMAMVSQGSKGLVPVMLSIAL
                     QFFVCVALHARLYDLRPATGQLTRFYLVMSAGGALGGLFTALIAPLVFDWAWEHPLLV
                     LAAALLLPRAALPDWRAMQGLEPAMQRLLVAAVAISLGVLLALLFAITTQYEETVWRW
                     IYAGAIALLGLALVPWRAIGPILLAVVMYVQGGFDTIADSRNGVRTRSYFGIYTVRDY
                     PQHKLRTLAHGTTLHGEQSTDPALRRQIRSYYGPGSGAGVVFGHAPELFGTSPAIGVV
                     GLGTGTLACFNKPGEDWTFFEIDPAVLELSRKGTFTFLRDCAPQARIALGDARLELIR
                     QPPGSLDLLAIDAFSSDAIPLHLMTDEAFAIYLKALSRNGVLLVHISNRYIDLEPVIS
                     ALARRRGLHAAIRDDAPEGDLTKIYTGSTWIALSRDAGQLERIAKLAPQLEWQPLATP
                     APRVWSDDHASILPFIRWENFLGTY"
BASE COUNT          522 a         1061 c         1032 g          521 t
ORIGIN      
        1 atcgcgatca tcatcgcctc gagccagctc aaggactttt tcggcttttc cggcgccgcc
       61 ctgccggccg aattcatccc caagctggcg gcgctgtggg cggttcgcgg cacgttcaat
      121 gccaccgccc tcttgatcgg gatcgcggcg ctgatcctga tcgtgctgct tcggcgcctg
      181 ttcccccgct ttccaggatt gatcgtggca atgaccgtgg tttccgcggc ggtcgcgctg
      241 gggcacttgc cggtcgatac gatcgcatcg cgctatggcg ccctgcccgg tcacctgccc
      301 atgcctgcag tgcccgcggt gtcgctcgag cggctcgggg acctgctgcc ctcggcgctg
      361 gtaatcgcgt tcctggccgc gatcgaatcg ctgctttcgg ccatggttgc cgatcgcatg
      421 atcgaaggca gccaccgccc caatgccgaa gtgatcgccc agggttgggc caatattgcc
      481 tcggcgctgt tcggcgggct tcccgccacc ggcgcgattg cacgcaccgc caccaacatc
      541 cgggccgggg ctaccacccc ggttgccggg ctggtccatg ccatcgtcat cctgctggtc
      601 ctgctggtcg ctgcgccgct ggcggggttt ctggccatgc ctgcgctcgc cgcgctactc
      661 ctgacaacgg cgtggaacat gagcgagccg cacaagtggc gatcctattg ggcggcaccg
      721 gcaggcgacc ggttgctact gctgatcact ctggtgttga cggtgctgag cgacctgacc
      781 gtggcgatcg gcgtcggggt ggcgcttggc ctggcgctgc gtctgcgcga tcgccagctt
      841 gaagcggatc gctggcgtac gcccgagcgc taggatcgat cgatccgcac cagcttgtcg
      901 cgcccggtcg cgccgcgcag cagggtgagg cgcgagggcg ccagatccag cgctttggcc
      961 agcagatcga gcaccgcagc attggccttg ccatcttcgg gcctggcccg gaccttgatc
     1021 agcacctggc caccctcgat tgcaatgctc tcggtccggg caccgggcgt taccctcagc
     1081 gccagccgtc cttcaggatc gacctgcgca aggattgcct gcggatcggg cagatcagcc
     1141 ctgggccgag ccatcgtaga gcgcctggtg agccctggcg ttctcgacat agtgcttcac
     1201 gcccatttgc aatccggcca gcgccgcatc ggtcagttcg cgcatgaagg tggcggggcg
     1261 gccgctccac agctggcgcg cggggatccg cttgcccggt gcgagcagag cgccggcggc
     1321 gagcatcccg tcggcctcga tcacgcagcc gttcatggtc tgcgcggcga agccgacaaa
     1381 cccgcggtcc tcgatcaccg tgccatggat catcgccatg tgaccgatca gcacatcgtc
     1441 gccgataagg gtgggatagc catcgggggc gccaggcata gccgcatcgc aatggatcac
     1501 gctgccatcc tggacattgg tgcgcgcgcc gatcaccacc cggctggcat cgccgcggat
     1561 cacgcaattg taccagatgc tggcgtcagg tccgatctcg acatcaccga tgatccggca
     1621 ccccggagca atgaaggcgc tgtcatggat cctgggcgac ttgccatgga tgccaaggat
     1681 cgtggtgtcc ggtcggggtg tcgtcatcag taggttccca gaaagttttc ccagcggatg
     1741 aagggaagga tcgaggcgtg gtcgtcgctc cacacccgcg gcgcgggtgt ggcaagcggt
     1801 tgccattcaa gctgcggggc aagtttggcg atccgttcca attgcccggc atcgcggctc
     1861 agggcaatcc aggtcgagcc ggtgtagatc ttggtcagat cgccttcggg cgcatcgtcg
     1921 cgaatcgccg catgcagccc gcgccgcctt gccagcgcgg aaatgaccgg ttcgagatcg
     1981 atatagcgat tggagatgtg caccagcagc acaccattgc gcgacagcgc cttgaggtag
     2041 atcgcgaagg cctcgtcggt catcaggtgc aacggaatcg cgtccgagga aaaggcatcg
     2101 atcgcgagca gatcgagact gccgggcggc tgccggatca gttcaagccg ggcatcgccc
     2161 agcgcgatgc gggcctgggg cgcacaatca cgcaggaagg taaaggtgcc cttgcgcgac
     2221 agttcgagca cggcgggatc gatctcgaag aaggtccagt cttcaccggg cttgttgaag
     2281 caggcgagcg tgcccgtgcc aagcccgacc accccgatcg ccgggctggt cccgaacagc
     2341 tcaggggcat gtccgaacac cacgcccgcc cccgagcccg ggccatagta ggagcggatc
     2401 tgccggcgca gcgccggatc ggtcgactgt tcaccgtgca gcgtggtgcc gtgtgccagg
     2461 gtgcgaagct tgtgctgcgg atagtcgcgc accgtgtaga tcccgaaata gctgcgcgtg
     2521 cgcaccccgt tccggctgtc ggcaatggtg tcgaacccgc cctggacata catcaccacg
     2581 gccaggagga tcgggccgat cgcccgccac ggcaccagcg ccagccccag aagggcgatt
     2641 gccccggcat agatccagcg ccagacagtt tcctcgtact gggtggtgat ggcgaacagc
     2701 agcgccagca gcaccccaag gctgatcgcc acggccgcca cgagcaggcg ctgcatcgcc
     2761 ggttccagcc cctgcatcgc ccgccagtcc ggcagtgccg cgcgcggaag cagcagcgcc
     2821 gcggccagca ccagcagcgg gtgttcccag gcccagtcga acaccagcgg cgcgatcagc
     2881 gcagtgaaca gcccgcccag cgcgccgccc gccgacatca ccaggtagaa gcgcgtcagc
     2941 tgcccggtgg cggggcgcag gtcatagagc cgggcatgca gcgcgacgca gacgaagaac
     3001 tgcaaggcga tcgaaagcat gaccggaacc agccccttcg atccttgcga aaccatcgcc
     3061 atcccgccgg cccccagcag caccggccag gtcagcgcgg cgatgatccg tgcggggcgg
     3121 cgcccttcgg caaagg
//
LOCUS       DASUDV010000070         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1173243,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000070 DASUDV010000000
VERSION     DASUDV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173243"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..869)
                     /locus_tag="VFV30_01515"
     CDS             complement(153..869)
                     /locus_tag="VFV30_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846796.1"
                     /translation="MWAIVLLAACQKDQDGSAATAPQSAVPPTQPVASAAAKAPDLGA
                     CPKPKLLADLSQEREKPLILPDAAAGLFVADRYRMAVSSLSGATICIDTYGIEGISNA
                     KVSPDKRFISFDWGGFEAFGHSIIDRSGRGQEVDTGTAPLTTADGRRMAAVDLSESGF
                     GSLNAFAVWDIDPTGIKEIARFSEGLPDLGQWTATGWSGDTCVNLEFDGWDEQAGKSR
                     RQPWRAAAGKGWRPEPGSCP"
     gene            complement(893..>2111)
                     /locus_tag="VFV30_01520"
     CDS             complement(893..>2111)
                     /locus_tag="VFV30_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX4846797.1"
                     /translation="QEHLTGPEGAIGRMVAAGKLSSMILWGPPGTGKTSTARLLADAV
                     GMRFLSVSAVFSGVADLKKAFAEAEVAAKAGQRTLLFVDEIHRFNRAQQDGFLPFVER
                     GTVTLVGATTENPSFALNAALLSRAQVLILHRLDAAALDTLLARGEELEGPLPLTADA
                     RAALVASADGDGRFLLNQAETLYNAKLTEPLDPAGLGKFLQRRVAVYDKDRDGHYNLI
                     SALHKALRGSDPQAALYYMARMLTAGEEPLYVLRRLVRFASEDIGLADPQALTQCLAA
                     KDAYQFLGSPEGELAIVQACLYLATAPKSNAAYAAQKAAWQSARETGSLAPPANILNA
                     PTKLMKDIGYGKDYAYDHEAEDGFSGANYWPDGMEPQAYYQPVERGFEREVLKRLDWW
                     EKKRREIRGESGQ"
BASE COUNT          326 a          726 c          704 g          355 t
ORIGIN      
        1 cagccaggcg aggacctccg ggcgggacca gcggtggccg ggccggacca gcgccggcgc
       61 cgcctcgccc gcgcgcagca gcgccacggc aatcccccgg tgccgctcgc ccgccgcacc
      121 cgaccagtcg atcgcggcga agtggcggaa cgctaggggc acgacccggg ctcgggcctc
      181 caacccttgc cggctgcggc gcgccagggt tggcggcggc tcttgccagc ttgctcgtcc
      241 cacccgtcaa attccagatt cacgcaggtg tcgcccgacc agccggtagc cgtccattga
      301 cccagatcgg gcaacccctc gctgaagcgg gcaatctcct tgattccggt tgggtcaatg
      361 tcccagacgg caaaggcatt gagcgagccg aaccccgact cgctcagatc gacagccgcc
      421 atgcgccgcc catcggccgt ggtcagaggt gccgtgccgg tatccacttc ctggccgcga
      481 cccgatcggt cgataatgct gtggccaaag gcctcaaagc cgccccagtc aaagctgata
      541 aatcgcttgt cgggcgagac cttcgcattg gagatcccct cgattccgta ggtgtcgatg
      601 cagatcgttg cgccggacag gctcgaaacc gccatacgat agcggtcagc cacgaacagg
      661 cctgccgctg catcgggcag gatcagcggc ttctcgcgct cctgcgacaa atccgccagg
      721 agctttggtt ttggacaggc accgagatcg ggagccttgg cggcggcact ggcaaccggt
      781 tgggttggcg gaacagcgct ttgcggggca gttgcggctg atccgtcctg gtccttctga
      841 cacgctgcca aaaggacgat ggcccacaca gttccaatgc ctctccggtg cttcattgtc
      901 cgctttcgcc ccggatctcg cgccgcttct tctcccacca gtccagccgc ttcagcactt
      961 cgcgttcgaa cccgcgttcg accggctggt aataggcttg cggctccatc ccgtccggcc
     1021 agtaattcgc gccggaaaag ccgtcctcgg cctcgtggtc gtaggcgtag tccttgccgt
     1081 aaccgatgtc cttcatcagc ttggtcggcg cgttgaggat gttggcgggc ggggccaggc
     1141 tgccggtttc cctcgcgctc tgccaggcgg ccttctgcgc ggcataggcg gcgttcgatt
     1201 tgggcgcggt ggcgaggtag aggcaggcct ggacgatcgc cagttcgccc tccggagagc
     1261 cgaggaactg gtaggcgtcc ttggcggcga ggcactgggt cagcgcctgc ggatcggcca
     1321 ggccgatgtc ttcagaggca aagcggacca gccggcgcag cacgtagagc ggctcttccc
     1381 cggcggtcag catccgcgcc atgtaataga gcgcggcctg cggatcggac ccgcgcagcg
     1441 ccttgtggag cgcggaaatc aggttgtagt ggccgtcgcg gtccttgtca tagaccgcca
     1501 cccggcgctg caggaacttg ccgaggcccg cgggatcgag cggctcggtc agcttggcgt
     1561 tgtagagcgt ttcggcctgg ttgagcagga accgcccgtc accatcggcg ctggcgacca
     1621 gcgccgcacg ggcatcggcg gtcagcggca gcgggccttc cagctcttcg ccgcgggcca
     1681 gcagggtatc gagcgcggcg gcatccagcc ggtgcaggat cagcacctgc gcgcggctga
     1741 gcagcgccgc gttgagcgcg aagctggggt tctcggtcgt cgcgccgacc agggtcaccg
     1801 tgccgcgttc cacaaagggc aggaacccgt cctgctgagc gcggttgaag cggtggatct
     1861 cgtccacgaa cagcagggtg cgctggccgg ccttggccgc cacttcggcc tcggcaaagg
     1921 ccttcttgag gtcggccacg cccgaaaaga ccgcgctgac cgacaggaac cgcattccca
     1981 ccgcatcggc cagcagccgc gccgtgctgg tcttgccggt gccgggcggg ccccacagga
     2041 tcatgctgga cagcttgccc gccgccacca tccgcccgat cgcgccttcg ggaccggtca
     2101 ggtgttcctg c
//
LOCUS       DASUDV010000071         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_466206,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000071 DASUDV010000000
VERSION     DASUDV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466206"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFV30_01525"
     CDS             <1..575
                     /locus_tag="VFV30_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX4846798.1"
                     /translation="ADHRAARLEEDALIMLRRVLSAVLLAWAIGFVWFAVALPQPAPA
                     GKSDGVVVLTGGKGRIDRGLVALRRGWSERMLVSGVGAEVKPGEFAAQYKVNAALMAC
                     CVELGYEAVDTRSNAREAADWIAQNKVRSVRLVTSDWHMRRAAMELRAAAPAGLTIDE
                     DAVPSQPSLRTLFLEYHKLLARLLSRAVWA"
     gene            577..1260
                     /locus_tag="VFV30_01530"
     CDS             577..1260
                     /locus_tag="VFV30_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4846799.1"
                     /translation="MHVLRSLAFYFAFYVGGAFLIAFAGLMMFAPRPAFRWAVDTWSG
                     YHRLCARLFLGIRVRVEGAMPTGPVLIAIKHESFFEAIDLPHLLDYPGVFAKAELMSI
                     PVWGLAGRRYGLVEVHREQGAKALRQMLAAARALSDAGRPLAIFPEGTRVPHGEQREL
                     KSGFAGVYKLLGLPVVAVAVNSGPLYHRWWKCSGTLTYRFSEPIPPGLPRDEAEARVI
                     AAINALNHE"
     gene            1257..2513
                     /locus_tag="VFV30_01535"
     CDS             1257..2513
                     /locus_tag="VFV30_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX4846800.1"
                     /translation="MSANKPKRSIFSVLGPGLVTGAADDDPSGIGNYSQVGAQFGYGL
                     AWTMAFGFPLLASIQAICARIGATTGKGIAQNLRQHYSPWLLRVVVVMLLTANVINLG
                     ADLGAMGAVLALVVPGPVLAYTAAFGLLCVVLEVFLSYGQYARILKWTTLSLFAYVGV
                     VFVADVPWRIALMNIAVPSFTFDRDHAMALVAIFGTTISPYLFFWQAGQEVEEQHRRH
                     IKPLHVSPRRAGAELKRIRTDTLVGMGFSHLVALFIIVATAATLNANGVTDIASAAQA
                     AQALRPIAGDFAFALFAIGIIGTGLLAVPILAGSAAYALSETFGWVEGLDRKPREAKA
                     FYGAIAAATLGGIALNFIQIDPMRALYWAAVVNGLLAPPLMVVTMLIARNRKIMGNLA
                     ISSKLELGGWLSTAVMWLIAGVFLFS"
     gene            complement(2510..3421)
                     /locus_tag="VFV30_01540"
     CDS             complement(2510..3421)
                     /locus_tag="VFV30_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEX4846801.1"
                     /translation="MSFDSVAIIGLGLLGGSVGLAVREHLPQVRTTGYDADPATRARA
                     AERGLVADVCDSIGDAVRDADLVILCVPVGAMASAAAEIAPHLKPGVLISDVGSSKQS
                     VARALGDALPGVAVIPAHPVAGTERSGPDAGFASLFQKRWCILTPPADADPALLAGLS
                     AFWEALGAKVEIMDAQHHDLVLAVTSHLPHLIAYTIVGTASDLEGVTESEVIKYSAGG
                     FRDFTRIAASDPTMWRDVFLSNKDAVLDMLQRFTEDLTALQRAIRVGDGAQLFDHFTR
                     TRTIRRSIIEEGQDDARPDFGRSDHAD"
     gene            complement(3418..>3565)
                     /locus_tag="VFV30_01545"
     CDS             complement(3418..>3565)
                     /locus_tag="VFV30_01545"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682922.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX4846802.1"
                     /translation="LKGIAEAGYAVRWLPGQGLPNALRITIGLPHEMDAIARALRDMA
                     ETGQ"
BASE COUNT          574 a         1095 c         1205 g          691 t
ORIGIN      
        1 gcgcggatca ccgtgctgcg cgccttgaag aagatgctct gatcatgttg cggcgggtct
       61 tgtctgcggt gttgctggcc tgggcgatcg gctttgtctg gtttgccgtg gcgctgccgc
      121 agccggcacc ggcgggcaag agcgacgggg tggtggtcct taccggcggc aagggccgga
      181 tcgatcgcgg cctggtggca ttgcgccggg gctggtccga acggatgctg gtgtcagggg
      241 tcggggccga ggtgaagccc ggggaattcg ctgcacaata caaggtcaat gccgcgctga
      301 tggcctgctg cgtggagctt ggctacgaag cggtcgatac ccgttccaat gcccgcgagg
      361 cggccgactg gattgcccag aacaaggtcc gctcggtcag actggtcact agcgattggc
      421 acatgcgccg ggcagcgatg gaactgcgcg ccgctgctcc ggcagggctg acgatcgacg
      481 aggatgccgt gccgagccag ccttcgctgc gcaccctgtt ccttgaatat cacaaactgc
      541 tggcccggtt gctgagccgg gcggtatggg cctgacatgc acgttctgcg cagccttgcc
      601 ttctattttg ccttctacgt cggcggtgcc ttcctgattg cttttgccgg actgatgatg
      661 tttgcgccgc gcccggcatt tcgttgggcg gtcgatacct ggtctggcta tcaccggctt
      721 tgcgcgcgcc ttttcctggg catccgggtg cgggtcgaag gagccatgcc caccgggccg
      781 gtgctgatcg cgatcaagca cgaaagcttt tttgaggcga tcgatctgcc gcacctgctt
      841 gactatccgg gggtcttcgc caaggccgaa ttgatgagca tcccggtctg ggggctggcc
      901 gggcggcgtt acgggctggt cgaggtacac cgcgaacagg gggccaaggc cttgcggcag
      961 atgctggcag ccgcacgcgc cttgagcgac gcgggacgtc cgttggcgat tttccccgaa
     1021 ggaacccggg taccccatgg cgaacagcgt gaactcaagt cgggcttcgc cggagtctac
     1081 aagttgcttg gtctcccggt cgtggcggtc gcggtcaatt cagggccgct ctaccatcgc
     1141 tggtggaagt gcagcggtac tctcacctac cggttttccg aaccgatccc gcccggcctg
     1201 ccgcgcgacg aggcggaagc cagggtgatc gccgcaatca acgcgttgaa ccacgagtga
     1261 gcgcgaacaa accaaagcgg tcgattttct cggtactggg accggggctt gtcaccggcg
     1321 cggcagatga cgatccgtcg gggatcggca attacagcca ggtcggggcg cagttcggtt
     1381 atggcctggc ctggaccatg gccttcggat ttcccttgct ggcctctatc caggcaatct
     1441 gcgcgcggat cggcgcgacc accggcaagg ggatcgccca gaacctgcgg cagcattact
     1501 cgccgtggct gctgcgcgtc gtcgtggtca tgctgcttac cgcgaacgtg atcaacctgg
     1561 gcgccgatct tggggcaatg ggcgcggttc tggcgctggt cgtgcctggg cccgtgctgg
     1621 cctataccgc cgcgttcggg ctgctttgcg tggtgctcga ggtattcctg agctacggac
     1681 aatacgcccg gatcctcaag tggaccacgc tttcgctgtt cgcctatgtc ggggttgtgt
     1741 tcgttgccga tgtgccctgg cgcatcgccc tgatgaacat cgcggtgccc agcttcacct
     1801 tcgatcggga ccacgccatg gcgctggtcg caatctttgg caccacgatc agcccgtacc
     1861 tgttcttctg gcaggcgggc caggaggtgg aagaacagca tcgccgccat atcaagccgt
     1921 tgcacgtatc gccgcgccgt gccggggcag aactgaaacg gatccgtacc gataccttgg
     1981 tggggatggg cttttcgcac ctggttgcgc tgttcatcat cgttgccact gcggccacgc
     2041 tgaacgccaa cggggtgacg gacatcgcca gcgcggccca ggcagcccaa gcactgcggc
     2101 cgatcgccgg cgattttgcc ttcgcgctgt tcgcaatcgg gatcatcggc accgggctgc
     2161 tcgcagtgcc gatcctggcc ggcagcgcgg cctatgcctt gagcgaaacc ttcggctggg
     2221 tcgaaggtct tgaccgcaag ccacgcgagg caaaggcttt ctatggtgca atcgccgcag
     2281 caacgctggg cgggatcgcg ctgaacttca tccagatcga tccgatgcgc gcgctttact
     2341 gggccgcagt ggtcaatggc ctgcttgctc cgccgctgat ggtggtgacc atgctgatcg
     2401 ccaggaaccg gaaaatcatg gggaatctgg caatctcaag taaacttgag cttggtggct
     2461 ggctgtctac cgcagtgatg tggctgatcg ccggagtgtt tcttttcagc tagtccgcgt
     2521 ggtcgctgcg gccgaagtca ggccgcgcat catcctggcc ttcctcgatg atcgaacggc
     2581 ggatcgtgcg ggtgcgggtg aagtgatcga acagctgcgc cccgtcgccc acccggatcg
     2641 cgcgttgcag cgcggtcagg tcttcggtga agcgctgcag catgtcgagc acggcatcct
     2701 tgttggaaag aaatacgtcg cgccacatgg tcgggtcgct ggcggcaatg cgggtgaaat
     2761 cccggaaacc accggcggaa tacttgatga cttcgctttc ggtaacgcct tcgagatccg
     2821 aagccgtgcc gacgatggtg taggcaatga ggtgcggcag gtggctggtg acagccagca
     2881 ccagatcgtg gtgctgggca tccatgattt cgaccttggc gcccagcgcc tcccagaatg
     2941 cgctgaggcc agccagcagc gccgggtcag catcggccgg cggggtgagg atgcaccagc
     3001 gcttctggaa caggctggcg aaaccggcgt cggggcccga gcgttcggtc ccggccaccg
     3061 ggtgggccgg gatcaccgcc acgccgggca gggcatcgcc cagcgccctg gcaacgcttt
     3121 gcttggatga gccgacatcg ctgatcagga cgccaggttt gaggtgcggg gcaatttcgg
     3181 cagcggcgct ggccatggcg ccaactggca cgcacaagat caccagatcg gcgtcgcgca
     3241 ccgcatcgcc gatactgtcg cacacatccg ccacaagccc gcgttcggcg gcgcgggcgc
     3301 gggtggcggg atcggcatcg tagccggtgg tcctgacctg cggcagatgt tcgcgcacgg
     3361 cgaggccgac cgagccgccc aagaggccca ggccgatgat tgcgacgcta tcgaaactca
     3421 ttgtccggtc tccgccatgt cgcgcagggc cctggcgatc gcgtccattt cgtggggcag
     3481 gccaatcgtg atgcgcaagg cattgggcag tccctggcca ggcagccagc gcaccgcata
     3541 gccggcttca gcaatcccct tcagc
//
LOCUS       DASUDV010000072         6301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2167286,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000072 DASUDV010000000
VERSION     DASUDV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167286"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFV30_01550"
     CDS             complement(<1..777)
                     /locus_tag="VFV30_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX4846803.1"
                     /translation="MASAASPARSKPAAPGPRALAPDEDVPHLPVDNPLFLTIGIMLA
                     SLIQVLDTTIANVAIPHMQSSLGSTPDEISWVLTSYIVAVAVFMPITGWLADRIGSRR
                     LFIWSVSGFIVSSMLCGMAQNVTEMVIFRALQGATGAFISPLAQAAMIDTNKPSRQPQ
                     MMALWGIGIMIGPILGPLLGGWLTENWNWRWVFYVNVPLGAISLAILLAALPSREVMR
                     RRFDLAGFAMIGVMLVSVQLLLDRGNHVDWFESAESWVYAF"
     gene            complement(793..1863)
                     /locus_tag="VFV30_01555"
     CDS             complement(793..1863)
                     /locus_tag="VFV30_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX4846804.1"
                     /translation="MAEADPRLSEAAAEATAPAPKPKRNWGRLALMLALPLILLVTGY
                     TYWRSLQGQVSTDNAYVHLDKISVSAQVGGQIVDVMVKENQQVKAGDLLFRIDPEPFQ
                     LQIAQADAAIATAQASQIAAANASALSGADISAADQNIAFARSNLARQEALWKKGFTT
                     KADYEAAQHQVTVAEEQRRLAIAKRDEAAAKLATGRQIPGVYPQVASAQAQRRVAELS
                     LRRTEVRAPVSGQISQAGRLQPGQQIVSGVPVLTLVADGTAYIEANFKETDLAEMRVG
                     QPAEIRFDAYPGLVLKGHVASIGAGTGSEFSVLPAQNATGNWVKVTQRVPVRIVLDEA
                     SPRPLISGLSTKVTVFTDGRKR"
     gene            complement(1856..2302)
                     /locus_tag="VFV30_01560"
     CDS             complement(1856..2302)
                     /locus_tag="VFV30_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX4846805.1"
                     /translation="MELSLAYLLSDSARLLRRAFDARVRELGMTSPQARLLLILNVTE
                     GENQGYYAERLEVEPISLTRMIDRMEESGLIERRRDPADRRAWRLFLTARSRDVIDQV
                     RAGLAGLEDEMLAGLGAAERAALGTMLEAIRTNFTDARAALEVANG"
     gene            2433..3410
                     /locus_tag="VFV30_01565"
     CDS             2433..3410
                     /locus_tag="VFV30_01565"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX4846806.1"
                     /translation="MAIKTRITELLGIEHPIVQGGMQWVGVAEMASAVSNAGGLGILT
                     GLTQPSAAALAAEIERCKGMTDKPFGVNMTVFPTINPPDYKAYTQAIIDSGVKIVETA
                     GTPAVRELWEMMKPHGIKILHKCTAVRHALSAERAGVDVISIDGFECAGHPGEDDIPG
                     LILIPAAADKVKIPLLASGGFGDGRGLAAALALGADGINMGTRFTATVEAPIHQAFKD
                     AMVANDERSTDLIFRTFRNTARVAKNDISQQVVAAEREGKEFAAVADLVKGVRGREGL
                     QTGDTNHGIWSAGMIQGLIHDIPTCEVLVSRIVADAENIIRQRLAGMLA"
     gene            3425..4681
                     /locus_tag="VFV30_01570"
     CDS             3425..4681
                     /locus_tag="VFV30_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX4846807.1"
                     /translation="MAEAYIIDAVRTPRGVGKPGKGALSHLHPQHLAATVLKAIRDRN
                     NIDTSTVDDIIWSTSSQNGKQGGDLGRMAALAAGYDISASGTTLDRFCGGGITSVNLA
                     AATVMSGMEDCVIAGGTEMMSFTATYGAELANAGIRPLGMGSGNAALDAIHPQSHQGV
                     CGDAIAAREGIDRQALDALALVSQERADRAIREGRFDKSLITVYNEDGTVALDREEFP
                     RPETTMEGLSALKASFDGMANFDLGGGVTFKKQIQRVYPELDWQGVHHAGNSSGVVDG
                     AAALLITSKEYADKHGLKPRGRIVAYANQGDCPTLMLNAPVPAAKKVLEKAGLKKDDI
                     DHWEINEAFAVVAEKFIRDLDLDRTKVNPNGGAMALGHPIGATGSILIGTALDELERT
                     GGRYGLITMCAAGGMAPALIIERIDG"
     gene            4766..6151
                     /locus_tag="VFV30_01575"
     CDS             4766..6151
                     /locus_tag="VFV30_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4846808.1"
                     /translation="MTDLLAAIAEVVGPQGLLTGEDVSGRAADFMGLTRVQAKAIVRP
                     ASTAELSAVMKLCHAAGQVVVPAGGNTGLVQGNVCAPDDIQVSLERMRGIEEVDPVGR
                     TMTVGAGCPVQAAQEAAQEHGLLFPVDWGARGSATIGGGISTNAGGNSVVRYGMMRDS
                     VLGLEAVLADGTVISAMNHLIKNNAAYDLKHLFIGSEGTLGIVTRAVLKLQPAPLSTC
                     TALVALDSFDAVQTLFVEAGKRLGPGLTAFEVMWANFYEPIAIATGRHTAPLPGGHGF
                     YVIVELSGTDPDGDPPRFEALLADLIEREVVADAVLAASQTQAAQIWAIRDDIEALFG
                     YAMPSVTFDVSLPIRTMDGYVTALEASARAALGETMKLVVFGHMGDGNLHILLGAQPF
                     DAAMKHRIEELVFTPLQALGGSISAEHGIGLEKRQWLHLSRTPEELALMKTLKAALDP
                     LGLLNPGKVLP"
     gene            6157..>6301
                     /locus_tag="VFV30_01580"
     CDS             6157..>6301
                     /locus_tag="VFV30_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4846809.1"
                     /translation="MTYEVADHIATITLNRPERMNTISSAMLDELTRTLVRANEDEAV
                     RVVV"
BASE COUNT         1080 a         2094 c         2155 g          972 t
ORIGIN      
        1 gaaggcatag acccagcttt cggcggattc gaaccagtca acatggttac cgcggtccag
       61 cagcagctgg accgaaacca gcatcacccc gatcatggca aagccggcca gatcgaaccg
      121 ccgccgcatc acttcgcgcg aaggcagggc ggccagcagg atggccagcg aaatcgcgcc
      181 gagcggcacg ttgacataga acacccagcg ccagttccag ttctcggtca gccagccgcc
      241 cagcagcggg cccaggatcg ggccgatcat gatcccgatc ccccacagtg ccatcatttg
      301 cggctgacgg ctgggcttgt tggtatcgat catcgcggcc tgggccagcg gggaaatgaa
      361 cgccccggtc gccccctgca gcgcgcggaa gatgaccatt tcggtgacgt tctgggccat
      421 gccgcagagc attgacgaga cgatgaagcc ggacaccgac cagatgaaca ggcggcgcga
      481 tccgatccgg tcggccagcc agccggtgat cggcatgaac acggcgacgg cgacgatgta
      541 gctggtcagg acccagctga tctcgtccgg ggtcgaaccc aggctggatt gcatgtgcgg
      601 gatcgcgaca ttggcgatcg tggtgtccag cacctggatc agcgaggcca gcatgatccc
      661 gatggtcagg aacagcgggt tgtcgaccgg caggtgcggg acgtcctcgt ccggcgccag
      721 cgcgcgcggg cccggggcgg ccggtttgct gcgggcaggg ctggcggcgc tggccatcgt
      781 gccgctccgg ccttagcgct tgcggccgtc ggtgaagact gtgaccttgg tggacaggcc
      841 cgaaatcagc gggcgcgggc tcgcctcgtc cagcacgatc cgcaccggca cgcgctgggt
      901 caccttcacc cagttgccgg tcgcgttctg ggccggaagg accgaaaact cgctgccggt
      961 gccggccccg atcgaggcga cgtggccttt caggaccagg ccggggtagg cgtcgaaccg
     1021 gatctcggcc ggctggccaa cgcgcatctc ggccaggtcc gtttccttga aattggcctc
     1081 gatataggcg gtgccatcgg ccaccagggt aagcaccggc acgcccgaga cgatctgctg
     1141 gcccggctgg agccgcccgg cctggctgat ctgcccggat accggcgcgc gcacttcggt
     1201 gcggcgcagg ctcagctccg cgacccggcg ctgggcctgg gccgaggcga cctgcggata
     1261 gaccccgggg atctgccggc cggtggccag cttggcggcg gcctcgtcgc gcttggcgat
     1321 cgccaggcgg cgctgctctt cggccaccgt cacctggtgc tgggcggctt cgtaatcggc
     1381 cttggtggtg aagcccttct tccacagcgc ttcctgccgg gcgaggttgg agcgggcaaa
     1441 ggcgatgttc tggtccgccg cggagatgtc cgcgccggat agcgcgctgg cgttggcggc
     1501 ggcgatctgg ctggcctggg ccgtggcaat cgcggcgtcg gcctgggcga tctgcagctg
     1561 gaacggctcg ggatcgatcc ggaacagcag gtcgccggcc ttcacctgct ggttttcctt
     1621 gaccatcacg tccacgatct gcccgccaac ctgcgcggag accgaaatct tgtcgaggtg
     1681 gacataggcg ttgtcggtgc tgacctggcc ctgcaggctg cgccaatagg tgtagccggt
     1741 gaccagcaag atcagcggca gcgccagcat cagcgccagc cggccccagt tgcgcttggg
     1801 cttcggcgca ggcgcggtcg cttcagcggc ggcttcggac agacggggat cggcttcagc
     1861 cattggccac ctccagcgcg gcgcgggcat cggtgaaatt ggtccggatc gcttccagca
     1921 tggtgcccag cgcggcccgt tcggcggcgc ccaggcccgc cagcatctcg tcttccagcc
     1981 cggccaggcc agcgcggacc tggtcgatca cgtcgcggct gcgggcggtc aggaacaggc
     2041 gccaggcgcg gcggtcggcg ggatcgcggc ggcgttcgat caggccggat tcctccatcc
     2101 ggtcaatcat ccgggtcagc gagatcggct ccacctccag ccgctcggca tagtaaccct
     2161 ggttctcccc ctcggtcacg ttgaggatca gcagcaggcg ggcctgcggg ctggtcattc
     2221 ccagttcgcg cacccgcgca tcgaaggccc ggcgcagcag gcgcgcgctg tcggacagca
     2281 gataggcaag actcagctcc atgctgcaca tataatagac gtgcttatga taagcaaggc
     2341 ttgccagatc gcgccaagtc tgctcaaggc gattgcgaat tttaactgat cggttaaacc
     2401 gactcgcaaa gaggcatgag aggagtcccc cgatggcgat caagacccgc atcaccgaac
     2461 tgctcgggat cgagcatccg atcgtccagg gcgggatgca gtgggtcggc gtggcggaga
     2521 tggccagcgc cgtttccaac gccggcggcc ttggcatcct gaccggcctg acccagccca
     2581 gcgctgctgc cctcgcggcc gagatcgagc gctgcaaggg catgaccgac aagccgttcg
     2641 gcgtgaacat gaccgtgttc cccacgatca acccgccgga ttacaaggcc tatacccagg
     2701 cgatcatcga cagcggggtc aagatcgtcg aaaccgccgg cacgcccgcc gtgcgcgagc
     2761 tgtgggagat gatgaagccc cacgggatca agatcctgca caagtgcacc gccgtgcgcc
     2821 acgccctttc ggccgagcgc gccggggtcg acgtgatcag catcgacggg ttcgaatgcg
     2881 ccggccaccc cggtgaggac gacattccgg gcctgatcct gatccccgcc gcggccgaca
     2941 aggtgaagat cccgctgctg gccagcggcg gctttggcga cgggcgcggc cttgccgccg
     3001 cgctggcgct gggggccgac gggatcaaca tgggcacgcg ctttaccgcc acggtcgagg
     3061 cgccgatcca ccaggccttc aaggacgcga tggtggcgaa cgacgaacgc tcgaccgatc
     3121 tgattttccg caccttccgc aacaccgcgc gggtcgcgaa gaacgacatc agccagcagg
     3181 ttgtggcggc ggagcgtgaa ggcaaggaat tcgccgccgt ggccgatctg gtcaagggtg
     3241 tgcgcggccg cgagggcctg cagaccggcg ataccaacca cgggatctgg tcggccggga
     3301 tgatccaggg cctgatccac gatatcccga cctgcgaagt tctggtcagc cggatcgtgg
     3361 ccgacgcgga aaacatcatt cgccagcgcc ttgccggcat gctggcctga gaaggagaga
     3421 accaatggcc gaagcctata tcatcgacgc cgtccgcacc ccgcgcgggg tgggcaagcc
     3481 cggcaagggc gcgctttcgc acctccaccc gcagcacctg gccgccacgg tgctgaaggc
     3541 gatccgcgat cgcaacaaca tcgacaccag cacggtggac gacatcatct ggtcgacctc
     3601 cagccagaac ggcaagcagg gcggcgacct gggccgcatg gccgcgctgg ccgccggtta
     3661 tgacatcagc gcctcgggca ccacgctcga ccggttctgc ggcggcggga tcacctcggt
     3721 caacctggcg gcggccacgg tcatgtcggg catggaagac tgcgtcatcg ccggcgggac
     3781 cgagatgatg agcttcactg ccacctacgg cgcggaactg gccaatgccg ggatccgtcc
     3841 gctgggcatg ggttcgggca acgccgcgct cgatgcgatc catccgcaga gccaccaggg
     3901 cgtgtgcggc gatgcgatcg cggcgcgcga agggatcgac cgccaggcgc tcgacgcgct
     3961 ggcgctggtc agccaggaac gcgccgaccg cgcgatccgc gaaggccgtt tcgacaagtc
     4021 gctgatcacc gtctacaacg aagacggcac cgtggcgctg gaccgtgaag agttcccgcg
     4081 tccggaaacg accatggaag gcctctcggc gctcaaggcc agcttcgatg gcatggccaa
     4141 cttcgatctg ggcggcggcg tcaccttcaa gaagcagatc cagcgagtct atccggagct
     4201 ggactggcag ggcgtgcacc acgccggcaa cagctcgggc gtggtcgatg gcgcggccgc
     4261 cctgctgatc accagcaagg aatacgccga caagcacggc ctcaagccgc gcggccggat
     4321 cgtcgcctat gccaaccagg gcgattgccc gaccctgatg ctcaacgccc cggtcccggc
     4381 ggcgaagaag gtgctggaga aggccggcct caagaaggac gacatcgacc actgggaaat
     4441 caacgaggct ttcgcggtcg tcgcggaaaa gttcatccgc gatctcgatc tcgatcgcac
     4501 caaggtgaac cccaacggcg gcgcgatggc gctgggccat ccgatcggcg cgaccggttc
     4561 gatcctgatc ggcaccgcgc tggacgagct ggagcggacc ggcggccgtt atggcctgat
     4621 caccatgtgc gccgccggcg gcatggctcc ggccctgatc attgagcgca tcgacggctg
     4681 attctgcaac tcgtccgccg tttttgcgct tctcgccccg gccgtagctg atacggtcgg
     4741 ggcgaattgc ttcaagggga tgggaatgac cgacctgctc gctgcgattg ccgaagtggt
     4801 cgggccgcag ggccttttga ccggcgagga cgtgtccggc cgcgccgccg atttcatggg
     4861 gctgacccgg gtccaggcga aggcgatcgt ccgcccggcc agcaccgccg aactcagcgc
     4921 cgtgatgaag ctgtgccatg ccgccgggca ggtcgtggtg ccggccggcg gcaacaccgg
     4981 actggttcag ggcaatgtct gcgcgccgga tgacatccag gtctcgctgg aacggatgcg
     5041 cgggatcgag gaggtcgatc cggtcggccg gaccatgacc gtgggggccg gctgcccggt
     5101 ccaggccgcg caggaagccg cgcaggagca tggcctgctg ttcccggtcg actggggcgc
     5161 gcgcggcagc gccaccatcg gcgggggcat ttccaccaac gccggcggga actccgtggt
     5221 ccggtacggc atgatgcggg actccgtcct ggggctggaa gccgtgctgg ccgacggcac
     5281 ggtgatcagc gcgatgaacc acctgatcaa gaacaacgcc gcctatgacc tcaaacacct
     5341 gttcatcggc agcgagggca cgctggggat cgtcacccgc gcggtgctga agctgcagcc
     5401 cgccccgctg tccacctgca ccgcgctggt cgcgctggac agtttcgatg cggtgcagac
     5461 cctgtttgtc gaggccggca agcggctcgg gccgggcctc accgccttcg aagtgatgtg
     5521 ggccaacttc tacgagccga tcgcgattgc caccgggcgc cacaccgcgc cgctgcccgg
     5581 cgggcatggc ttctacgtga tcgtggagct gagcgggacc gatccggatg gcgacccgcc
     5641 ccggttcgag gcgctgctgg ccgacctgat cgagcgcgaa gtggtggccg atgcggtgct
     5701 ggccgccagc cagacccagg ccgcgcagat ctgggcgatc cgcgacgata tcgaggcgct
     5761 gttcggctat gccatgccca gcgtgacctt tgatgtcagc ctgccgatcc ggaccatgga
     5821 cggctatgtc accgcgctgg aggcctcggc ccgggccgcg ctgggcgaga cgatgaagct
     5881 ggttgtcttc ggccatatgg gcgatggcaa cctccacatc ctgctcggcg cgcagccgtt
     5941 cgatgcggcg atgaagcacc ggatcgagga gctggtcttc accccgctgc aggcgctggg
     6001 cggctcgatc agcgccgaac acggcatcgg tctcgaaaag cgccagtggc tgcatctcag
     6061 ccggacgccg gaggaactgg cgctgatgaa aaccctcaag gccgcgctgg atccgctggg
     6121 cctgctcaat cccggaaagg tgctcccatg accgacatga cctatgaagt ggccgatcac
     6181 atcgccacga tcacgctcaa ccggccggag cggatgaaca ccatctcctc ggccatgctg
     6241 gacgagctga cccgcaccct ggtccgcgcc aacgaagacg aggcggtgcg cgtggtggtg
     6301 c
//
LOCUS       DASUDV010000073         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_908075,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000073 DASUDV010000000
VERSION     DASUDV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908075"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFV30_01585"
     CDS             <1..856
                     /locus_tag="VFV30_01585"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX4846810.1"
                     /translation="FEAADGVAQVNLIGTELGRINLELARATPGSANQSALLDRRDAM
                     LEQLSSFGDVTTTFAPDQTVEVRLGGNGGPLLVSGVTVSSLAMTTAADGTISFTLGAA
                     PLALAGGSLAGKAQALVAVRDTALDLDALADSLIAAANGAQGAGVALDGSAGQPMFSG
                     SGAAGIALALTAGSQIATAPTGAGAGSRDPAGLNALINALDTADLAGQANGLLFAASS
                     RAAGSRLTAEALDTIAAGARIALDSQAGVNLDDEAVNLVRFQQAFQASSKAIQVASDV
                     IDTLLALN"
     gene            871..1785
                     /locus_tag="VFV30_01590"
     CDS             871..1785
                     /locus_tag="VFV30_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4846811.1"
                     /translation="MTAISTSAFYQRATRSLLSLRGEAERLQAAIGSGQRLTRSSDDP
                     LAAAQLRSLGRTDSFAAIDTTLGERVMADLTLADGALSSFAAYATRIKELAVQAANGV
                     LAPVQRQAIATELDQLRGELIGLANTRDAAGHALFGGETSGAAYALDAAGNPVYAGTA
                     SAGEVPLGDGQSVTRGVTGPEFLNFTAGGGPTDLFAVTKALADALRGAAPDPAQAARD
                     ALGSIDAGLDAITTTQTVLGTRMNWIELAAERRTAMAELRSEDQAELGGTDMAEAVLK
                     LQQTMTVLEASQASFARLSSLSLFAILR"
     gene            1819..>2353
                     /locus_tag="VFV30_01595"
     CDS             1819..>2353
                     /locus_tag="VFV30_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HEX4846812.1"
                     /translation="MFVAIGIVVLLLMVFGGFAITGGALGPVMHALPHEMLIIGGAAV
                     GAMITGNNAHGLKAIGKAFGTVFKGPRHTKQDHIDVIVLTTKLMKMLRAEGPVALESH
                     VTDPASSAIFAEFPRILANKELTGLICDTLTLIVVSSGTLEVHAVEEVMDHAMKTHFH
                     ELHEPQHALQGLADALPA"
BASE COUNT          391 a          811 c          792 g          359 t
ORIGIN      
        1 cttcgaggcc gccgacgggg ttgcccaggt caacctgatc ggcaccgaac tgggccggat
       61 caacctggaa ctggcgcgcg ccacgccggg cagcgccaac cagagcgcgc tgctcgaccg
      121 gcgcgatgcc atgctcgaac aactgtcctc cttcggcgat gtcaccacca cctttgcgcc
      181 tgaccagacg gtcgaggtgc gcctgggcgg caacggtgga ccattgctgg tcagcggagt
      241 cacggtgtcg agcctggcca tgacaaccgc cgccgatggc acgatcagct tcacactggg
      301 cgccgcgccg cttgcccttg cgggcggatc gctggctggc aaggcgcagg cgctggtcgc
      361 ggtacgcgat accgcgctcg atctcgatgc cctggccgac agcctgatcg ccgccgccaa
      421 cggcgcgcaa ggcgcgggcg tggcgcttga tggcagcgcg ggccagccga tgttttcggg
      481 atccggcgcg gcgggcatcg cgctggcgct caccgccggc agccagatcg ccaccgcgcc
      541 gaccggcgcc ggtgcgggca gccgcgatcc ggccgggctg aacgcgctga tcaacgcgct
      601 cgatacggcc gacctggccg gccaggccaa tggcctgctg tttgccgcat cgagccgggc
      661 ggcgggcagc cggcttaccg ccgaggcgct cgataccatc gctgcgggcg cgcggatcgc
      721 gctcgatagc caggccgggg tcaatctgga tgacgaggcg gtcaacctgg tgcgcttcca
      781 gcaggccttc caggccagca gcaaggcgat ccaggtcgcc tccgacgtga tcgacaccct
      841 gctggcgctg aactgaagaa aggaccggcc atgaccgcga tcagcaccag cgccttctac
      901 cagcgcgcca cccgcagcct gctttcgctg cgcggcgagg ccgagcggct gcaggccgcg
      961 atcggcagcg gccagcggct gacccgctcg tccgatgatc cgcttgccgc cgcgcagctg
     1021 cgcagccttg gccggaccga cagtttcgcc gcgatcgaca ccacgcttgg cgaacgggtc
     1081 atggccgacc tgaccctggc cgatggcgcg ctgtcaagct ttgccgccta tgccacgcgg
     1141 atcaaggaac tggcggtcca ggctgccaac ggcgtcttgg caccggtcca gcgccaggcg
     1201 atcgcaaccg agcttgatca gctgcgcggc gaactgattg gcctggccaa cacccgcgat
     1261 gccgcgggcc atgcgctgtt cggcggagag accagcggcg cggcctatgc cctcgatgcc
     1321 gcgggcaatc cggtctatgc cggaacggcc agcgccggcg aggtaccgct gggcgacggg
     1381 caaagcgtga cgcgcggggt aaccggccct gaattcctca atttcaccgc tggcggcggc
     1441 ccgaccgatc tgttcgcggt gaccaaggcg ctggccgatg cgctgcgcgg ggcggcgccc
     1501 gatccggccc aggcggcgcg cgatgcgctg ggttcgatcg atgccgggct ggatgcgatc
     1561 accaccacgc agacggtgct gggcacgcgg atgaactgga tcgaactcgc tgccgaacgc
     1621 cgcaccgcca tggccgaact gcgcagcgag gaccaggccg agctgggcgg aaccgacatg
     1681 gccgaagccg tcctcaagct gcagcagacc atgaccgtgc tcgaagccag ccaggccagc
     1741 ttcgcccggc tttcatcgct ttcgctgttc gcaatcctgc gctgaacccg cgcccaaatc
     1801 ccaggggtct gaaccaccat gttcgttgca atcggtatcg ttgtcctgct gctgatggtg
     1861 ttcggcggct ttgccatcac cggcggtgcg ctgggtccgg tgatgcatgc cctgccgcac
     1921 gaaatgctga tcatcggcgg ggcggcggtt ggggccatga tcaccggcaa caatgcccat
     1981 ggcctgaaag ccatcggcaa ggccttcggc acggtcttca aggggccgcg ccacaccaag
     2041 caggatcaca tcgatgtgat cgtactgacc accaagctga tgaagatgct gcgcgccgaa
     2101 ggtccggtgg cgctggaaag ccatgtcacc gatccggcca gttcggcgat cttcgcggag
     2161 tttccgcgca tcctggccaa caaggaactg accgggctga tctgcgacac gttgaccctg
     2221 atcgtggttt catcgggcac gcttgaagtg cacgcggtcg aggaagtgat ggaccacgcg
     2281 atgaagaccc atttccacga actgcacgaa ccccagcacg cgctgcaggg gctggccgat
     2341 gccttgcctg cac
//
LOCUS       DASUDV010000074         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_665032,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000074 DASUDV010000000
VERSION     DASUDV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665032"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1229
                     /locus_tag="VFV30_01600"
     CDS             324..1229
                     /locus_tag="VFV30_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4846813.1"
                     /translation="MATVEQGPGKAMQRPQRPRELQDPLNHYLYHPLAWQFARLLAPT
                     PITPNMVSVLGAGFVVAAAFAYTQLTWPVSAALGMILHMTWHVVDGADGDLARMTGRS
                     SPIGEMVDGLCDYLSHAVLYIALALLLAGGAGVFSGWAAWVLVIAAGISHAVQANHVE
                     VQRRQYQYWVYGTPWLRNSHGKEDSATSKSWAGALVSAYIGVASGMTPEALKVDAAVQ
                     AAGGDPTRLGAIADAARAEAPPLLRLCKILGPNPRAIVLGISMLAGSPLWYMLYQTIV
                     LNLLLVHSVRAHNAAARRIAAKIGA"
     gene            1232..2449
                     /locus_tag="VFV30_01605"
     CDS             1232..2449
                     /locus_tag="VFV30_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4846814.1"
                     /translation="MLHEALLEDARALEGEIVALRRAIHAEPELGLHTPKTRDKVRAA
                     LANLPLEWVEGPSTTGLVATLKGGAGEGRCVLLRGDMDALPMPEETGLDYASTIPGTM
                     HACGHDTHTAMLAGAVRLLCARRDRLKGEIRFMFQPGEEGFHGAKFMLEDGLLGGEGT
                     GRALPEAAFALHVMPNARHGQVAGRAGALMASADQFDITVTGRGGHAAMPYSALDPVP
                     IACEIVMALQTLVTRQFDANDPVICTVAKLEAGTAHNIIADFAKLQGTLRALSPRHRG
                     KLHASLEQLAKGIAAAHGAEASVVITPGFPVSVCDPRAVDLGEAVSRDLFGEGAFRRM
                     DQPMMGAEDFSYVLEQVPGAMFFLGVAHEGADWHSCPAIHSPRMTVDESALPKGAALL
                     AGCAERFLDHGWA"
BASE COUNT          486 a          971 c          974 g          482 t
ORIGIN      
        1 tcaggccctg cgaggaattg ttctggcggt aataggcgac gccctggacg agcgagcttg
       61 cgcagttacc gacgccgatc atggcgacct tgataggctt cattctgttt ctgtcccttt
      121 tcgagcggca cgacccgggc cgcgaaaggc ccggtattgg aaaaatcggt cgccaaaagc
      181 aagccaattg gcccgcgtca agccgaatgc gcccgaaagc ccccggatgc gacatttctg
      241 ttcgcatcgg caaggcggct gttgcgctgt gcgcgttggg ctttatcccc tcgaatcgca
      301 aggaatcact agggagtaac acagtggcaa cggtggaaca ggggccgggc aaggcgatgc
      361 agcggccaca acgcccccgc gaactgcagg acccgctgaa tcactatctt taccacccgc
      421 tggcctggca gttcgcccgt ttgctcgctc cgactccgat cacgcccaac atggtctcgg
      481 ttctgggggc cggattcgtg gtggcggcgg cctttgccta tacccagctg acctggccgg
      541 tctcggccgc gctggggatg atcctgcaca tgacctggca cgtggtcgat ggggccgatg
      601 gcgatctggc ccggatgacc ggccggtcca gcccgatcgg cgaaatggtt gacgggctgt
      661 gcgactatct cagccacgcg gtgctctata tcgccctggc gctgctgctg gccggcggcg
      721 ctggggtctt ttcgggctgg gccgcctggg tcctggtgat cgctgccggg atcagccacg
      781 cggtccaggc caaccatgtc gaggtgcagc gccgccagta ccagtactgg gtctatggca
      841 ccccctggct gcgcaacagc cacggcaagg aggattcggc cacctccaag agctgggccg
      901 gggcgctggt ttcggcctat atcggggtgg ccagcgggat gacccccgaa gcgctcaagg
      961 tggacgccgc ggtccaggcc gccgggggcg atccgaccag gctcggcgcg attgccgatg
     1021 cggcccgggc cgaagcgccg ccgctgctgc ggctgtgcaa gatcctgggc cccaacccgc
     1081 gggcaatcgt gctgggcatt tcgatgctgg ccggcagccc cttgtggtac atgctttacc
     1141 agaccatcgt tctcaatctg ctgctggtcc attcggtgcg ggcccacaac gccgccgccc
     1201 gccggatcgc cgccaagatc ggagcctgac aatgctgcac gaagccctgc tggaagacgc
     1261 ccgcgccctg gaaggcgaga tcgtggccct gcgccgcgcg atccacgccg aaccggaact
     1321 gggcctccac acgcccaaga cccgcgacaa ggtccgcgcc gcgctggcca acctgccgct
     1381 cgaatgggtc gaggggccca gcaccaccgg gttggtcgcc acgctgaagg gcggggcggg
     1441 cgaaggccgc tgcgtgctgc tgcgcggcga catggacgcc ctgccgatgc cggaagagac
     1501 cgggctcgac tatgcctcga ccattccggg cacgatgcac gcctgcgggc acgacaccca
     1561 caccgccatg ctggccgggg cggtgcgcct gctctgcgcc cggcgtgacc ggctgaaggg
     1621 cgaaatccgc ttcatgttcc agcccggcga ggaaggcttc cacggcgcga aattcatgct
     1681 ggaagacggc ctgctgggcg gcgaaggcac cgggcgggcc ctgcccgagg cggcctttgc
     1741 gctgcacgtc atgcccaacg cccggcacgg gcaggtcgcg ggacgggccg gggcgctgat
     1801 ggcctcggcc gaccagttcg acatcaccgt gacggggcgc ggcggccatg ccgcgatgcc
     1861 ctacagcgcg ctcgatccgg tgccgatcgc ctgcgagatc gtgatggcgc tgcagacgct
     1921 ggtcacccgc cagttcgatg ccaacgaccc ggtgatctgc accgtcgcca agctcgaggc
     1981 cggcaccgcg cacaatatca tcgccgattt cgccaagctg cagggcaccc tgcgcgcgct
     2041 ttcgccgcgg caccgcggca agctccatgc gtcgcttgaa cagctggcca aaggcatcgc
     2101 cgccgcccac ggggccgagg ccagcgtggt gatcaccccg ggctttccgg tctcggtctg
     2161 cgatccgcgc gcggtcgacc tgggcgaagc ggtcagccgc gacctgttcg gcgaaggcgc
     2221 cttccggcgg atggaccagc cgatgatggg ggcggaggat ttctcctatg tgctggaaca
     2281 ggtgccgggc gcgatgttct tcctgggcgt cgcccacgaa ggcgcggact ggcacagctg
     2341 cccggcgatc cacagcccgc gcatgacggt cgacgaaagc gccctgccaa agggcgcggc
     2401 cctgctggcc ggctgcgccg agcggttctt ggatcacggc tgggcgtaag actggagcac
     2461 ccgacaggat tgggcacgcg ctgcgcgcgc acccgtctgc gcttcgctcc gactccgaag
     2521 ggtccgaacc gtcctgatac gccaaagaca aagggcggcc ccttggaccg ccctttgtct
     2581 ttggcgcacc cgacaggatt cgaacctgtg acctctgcct tcggagtgca gagtttccag
     2641 acaaagcggg acaagctgat acgctgaaat ctctgacata aagccatttc ctatctctgc
     2701 ttgtttccga taatccccgg atgtattcca tacgcattcc gtacggaatg gaatgaggga
     2761 attcgcagtg gcattggacc tgagcaaagt tggcacccgg gggcggttga aagtgcaacg
     2821 cgagccgcac tggcaaaggc tgcgcgccgg atgcttcctt ggcttcaggc cctccaagcg
     2881 tggagggaaa gggacatgga tcgcccgcgt cta
//
LOCUS       DASUDV010000075         6858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_886071,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000075 DASUDV010000000
VERSION     DASUDV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886071"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFV30_01610"
     CDS             <1..319
                     /locus_tag="VFV30_01610"
                     /inference="COORDINATES: protein motif:HMM:NF021584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEX4846815.1"
                     /translation="LAVVDDPPEGVTDITFKYPLEGWEHIWVGNVRRYGNTLHGQLIN
                     VPEAEGHEIGERVVVPLSDVSDWGYRGADGVMRGNFTTRVLLKKVPPADAKALRDWLG
                     WTE"
     gene            complement(329..730)
                     /locus_tag="VFV30_01615"
     CDS             complement(329..730)
                     /locus_tag="VFV30_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846816.1"
                     /translation="MPALRAGLAYWGLIFALGFALGTVRTLWLAPAVGPLAAVGIELP
                     IMLGASLWLARRLIRRHGITASAEALVMSGTAFALLLISEAALARLLGGQSLADWAAD
                     LASPPGALGLAGQIAFALLPLVLVRRAPRGG"
     gene            complement(736..1173)
                     /locus_tag="VFV30_01620"
     CDS             complement(736..1173)
                     /locus_tag="VFV30_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846817.1"
                     /translation="MSETDLSGPPPSMTPNPNPGTPPPGPNPPSGSPPGGFDLNYPTI
                     VSLLYLASFILGVTALVGVVLAFVWKGESGRAPWEASHYQYLINTFWIGLIGFFVGFL
                     TLLLVVGAFILLGTMALVVVRCVLSLIRAQKHEPMPNPGTWLA"
     gene            complement(1248..2132)
                     /locus_tag="VFV30_01625"
     CDS             complement(1248..2132)
                     /locus_tag="VFV30_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX4846818.1"
                     /translation="MLFVSYATHDLERVRGLVSAIETRGIPCWYAARDIRPSEIFAKA
                     IADAVRASSGCVVVVSKASLASDGVLRELELTSSAGKPFFPILIEPCSLSAGFDYYLS
                     LAQWIDLSAEGEAGLDRLVAREAAAPPPGPVYPRGKRLFTPLRLGLGLASVGIAALLA
                     AASLRQPADPVAAMTPAEMRLEALDAIRRLDVDRMRRLIRAGWLANSQIDAAGSNALH
                     FLAETCEWNPDADPQKMVQFGRMLLDNGTSLIQVNVYDDTPLDIARAERFCGPDHALT
                     KFYTTMCTGKTGLSKACT"
     gene            complement(2269..2862)
                     /locus_tag="VFV30_01630"
     CDS             complement(2269..2862)
                     /locus_tag="VFV30_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846819.1"
                     /translation="MKPASFLFAFVLTGCATTPAASPQDAFWQELSRHCGKAFAGRLV
                     SNEAPDADMRGAAMVIHVRECSDRRIAVPFHIQRADGSWDRSRTWLITRTPAGLRLKH
                     DHRHEDGKPDSMTLYGGDTASAGTAEAQDFPVDAESVALFKAEGRSVSITNVWRVEVG
                     AGRFAYQLTRQPPNARLFRVEFDLTRPVPAPPAPWGH"
     gene            complement(2904..3311)
                     /locus_tag="VFV30_01635"
     CDS             complement(2904..3311)
                     /locus_tag="VFV30_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846820.1"
                     /translation="MRADIWQTGDVFVYGGLAFAVLCLALLIRHDWVRLTRPSRRVLA
                     EVIGHHSRFQGGAMMYSAIYGFYDETGAHEVTDAVTQAEPEPDEGETVELVYPAGRPD
                     LARPPRPLMWLGVYATFAFILGMTIAKLMGWLD"
     gene            complement(3301..4602)
                     /gene="wecC"
                     /locus_tag="VFV30_01640"
     CDS             complement(3301..4602)
                     /gene="wecC"
                     /locus_tag="VFV30_01640"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HEX4846821.1"
                     /translation="MKSDVKPDVCVVGLGYIGLPTAAVVARAGCKVHGVDVTQSVVDT
                     INRGEIHIEEVDLDGLVQGVVTRGLLKASTEMAPADVFIIAVPTPFDENHAPDISYVL
                     AAGEAVAKVLKAGDCVILESTSPVGTTEALRDLIAARRPDLKCPGLTGETPDIAISYC
                     PERVLPGRILEELTNNDRSIGGITPRCARKALSFYKLFVRGECVVTDSRSAEMTKLVE
                     NAYRDVNIAFANELSIVADKMGLDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWF
                     IVHGAPEETPLIRTARGVNDGKIHHVIARAEALIEAHPAARVACLGLAFKANIDDFRE
                     SPARAVAARLARKFGSRIKVVEPYASELPREFDGTAAELIDVDTALETCDVLIVLVDH
                     DAFKAVPLAERMTKAVYDTRGIWLDQPKALTEAAQGLRLAS"
     gene            complement(4586..5719)
                     /gene="wecB"
                     /locus_tag="VFV30_01645"
     CDS             complement(4586..5719)
                     /gene="wecB"
                     /locus_tag="VFV30_01645"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX4846822.1"
                     /translation="MTEPAKILVVFGTRPEAIKLFPVVAALREDPRFTPVVCVSAQHR
                     QMLDQVLEIAGLVPDHDLDLMKPDQTLDGLTAALLTGLGRVMDEVKPARVIVQGDTAT
                     AMTGALAAYYRQIPVDHVEAGLRSGNIYHPWPEEVNRKIIGTIASLHFAPTDVSAKAL
                     TDEQVDPARVHVTGNTVIDALHWVSARIAANPAMAAGLDELKARFADKRIIGVTSHRR
                     ENFGGGMEAIAEAIRQIAARPDVAVIFPVHLNPNVRKVMGAALDGLDNVALIEPLDYP
                     HFAHLLSLAEIMLTDSGGVQEEAPALGKPVLVMRETTERPEGVAAGTAKLVGTETAVI
                     VTELFNLLDDKAAYEAMARAHNPFGDGNSARRIVELIGNEIGR"
     gene            5802..6800
                     /locus_tag="VFV30_01650"
     CDS             5802..6800
                     /locus_tag="VFV30_01650"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX4846823.1"
                     /translation="MRVLVTGVAGFIGSTLALRLLERGDSVVGIDCINDYYQVSLKED
                     RLKNVTAAAGNRFAFHKVDFTDMAALDGALQGTEFDRIVHLGAQAGVRYSLENPQAYV
                     SANLAGHVNLLEIARHRQVEHMVYASSSSVYGGNTNMPFSVEDRVDHPVSLYAATKRA
                     DELMSETYAHQFRLPLTGLRFFTVYGPWGRPDMMMWIFTKAILAGQPIPVFNNGEMYR
                     DFTYIDDIVAGVIKCLDSPPADDGAEKAGGSVKPHALYNIGNNKSEHLMRVVELLEES
                     CGKKAEIDFQPIQPGDVPATYANIDAIQRDLGYAPTTSIDVGVPNFVRWYRDYHGV"
BASE COUNT         1176 a         2277 c         2332 g         1073 t
ORIGIN      
        1 cctggccgtg gtggacgatc cgcccgaagg cgtgaccgac atcaccttca agtacccgct
       61 ggaagggtgg gagcacatct gggtcggcaa cgtccggcgc tatggcaaca cgctccacgg
      121 gcagctgatc aacgtgcccg aggcggaagg gcatgagatc ggcgagcggg tggtcgtgcc
      181 gctgagcgat gtcagcgact ggggttatcg cggcgcggac ggggtgatgc gcgggaactt
      241 taccacccgc gtgctgctga agaaggtgcc cccggccgac gccaaggcgc tgcgcgactg
      301 gctgggctgg accgagtaag ccccgccctc agcccccgcg tggggcgcgc cggaccagaa
      361 caagcggcag cagcgcgaat gcgatctgcc cggcgaggcc cagcgccccg ggcggggaag
      421 cgaggtctgc cgcccagtcc gccaggctct gcccgcccag cagccgggcc agcgccgcct
      481 cgctgatcag cagcagggca aaggccgtgc cgctcatcac cagagcctct gccgatgccg
      541 tgatgccgtg gcggcggatc aggcggcggg ccagccacag actggcgccg agcatgatcg
      601 gcagctcgat tcccaccgcc gccagcgggc cgaccgcggg ggccagccac agggtcctta
      661 ccgtgcccag ggcaaagccc agcgcgaaga tcaggcccca ataggccagg cccgcgcgca
      721 gggccggcac ggcgatcagg ccagccaggt tccggggttg ggcatcggct cgtgcttttg
      781 cgcgcggatc agcgagagga cgcagcgcac cacgaccagc gccatcgtgc cgagcaggat
      841 gaaggcgccg accaccagaa gcagggtcag gaagcccacg aagaagccga tcaggccgat
      901 ccagaaggtg ttgatcaggt actggtagtg cgacgcctcc cacggcgcgc gcccgctttc
      961 gcccttccac acgaaggcca gcaccacccc gaccagcgcg gtcaccccga ggatgaacga
     1021 ggcaaggtag agcagcgaga cgatcgtcgg gtagttgaga tcgaacccgc ccgggggcga
     1081 gccggagggc ggattgggcc cgggcggcgg ggtgccgggg ttggggttcg gggtcatcga
     1141 cggcggcgga ccgctgagat cggtttcgga catggcagtt ccccccaagg cggttctcgt
     1201 tgcgggatca tgcccgccat gaccgcaagg tcaatcgcaa atccgcatca ggtgcaggcc
     1261 ttggacagcc cggtcttgcc ggtgcacatg gtggtgtaga acttggtcaa cgcatggtcc
     1321 ggcccgcaga accgctctgc ccgggcaatg tcgagcgggg tatcatcata gacgttgacc
     1381 tggatcagcg aggtgccgtt atcgagcagc atgcgcccga actgtaccat tttctgcgga
     1441 tcggcgtccg gattccattc acaggtctcg gccagaaagt gcagggcgtt gctgccggcc
     1501 gcgtcgatct ggctgttggc gagccacccg gcccggatca gccgccgcat gcggtcgaca
     1561 tcaagccgcc ggatcgcatc gagagcttca agccgcattt cggcgggtgt catcgccgcc
     1621 accggatcag caggctgacg caggctggcg gcggccagca gtgcggcgat gccgacactg
     1681 gccaggccca ggccgagccg cagcggagtg aagaggcgct tgccgcgcgg atagaccggt
     1741 ccgggcggcg gcgcggcggc ctcccgcgcc accagccggt caagcccggc ctcgccctcg
     1801 gccgaaaggt cgatccactg ggcgagcgag aggtaatagt cgaacccggc gctgagcgag
     1861 cagggctcaa tcaggatcgg gaagaacggc ttgcccgcgc ttgaggtcag ttccagttcg
     1921 cgcagcaccc catcggacgc cagcgaggcc ttgctgacca ccaccacgca gccggagctg
     1981 gcgcggaccg cgtcggcaat cgccttggcg aagatttccg atggccggat atcgcgcgcg
     2041 gcataccagc acggaatgcc ccgggtctcg attgccgaaa cgaggcccct gacccgttcc
     2101 agatcgtgtg tggcgtaact gacgaacagc atggcttggc gccgagacta tccgcgccgc
     2161 gcccggccga caagaagctt agctggcgag cttcagccgg tcgcgttcgg tctgcacggc
     2221 ccgtggctcc gcatcatgcc cgcatggcgg cgaaggtcaa tgccgggctt agtgccccca
     2281 gggcgcgggc ggcgcgggca ccgggcgggt cagatcgaac tccacccgga acagccgggc
     2341 gttgggcggc tggcgggtca gctgataggc aaagcggccc gcgccgacct ccacccgcca
     2401 gacattggtg atcgaaacgc tgcgcccctc ggccttgaac agggcaacgc tttccgcatc
     2461 gaccgggaaa tcctgcgctt cggccgtgcc ggcgctggcg gtatcgccgc cgtagagcgt
     2521 catcgagtcg ggcttgccgt cctcgtgccg gtgatcgtgc ttcagccgca agccagccgg
     2581 ggtgcgggtg atcagccagg tccggctgcg gtcccagctg ccgtcggcgc gctggatgtg
     2641 gaacggcacg gcgatccgcc ggtccgagca ttcgcgcacg tgtatcacca tggccgcgcc
     2701 gcgcatgtcg gcatcggggg cctcgttcga gaccagccgc ccggcaaagg ccttgccgca
     2761 atggcgcgac agctcctgcc agaaggcgtc ctgcgggctg gcggccggcg tagtggcgca
     2821 gccggtcagg acaaaagcga acaggaagga ggcaggcttc atcgccgcag cgtggcaggc
     2881 cgatcggccc ggggcaaggc tgctcagtcc agccaaccca tcagcttggc aatggtcatg
     2941 cccaggatga atgcgaaggt cgcatagacc cccaaccaca tcagcgggcg cggcggacgc
     3001 gccagatcgg ggcggcctgc gggatagacc agctcgaccg tctccccttc gtccggctcc
     3061 ggctcggcct gggtcacggc gtcggtgact tcgtgcgcgc cggtttcatc gtaaaagccg
     3121 tagatcgccg aatacatcat ggcgccgccc tgaaagcggg aatggtggcc gataacctca
     3181 gccagcaccc gccgcgaagg acgggtcagc cgcacccagt catgccggat cagcaaagcg
     3241 agacagagca cagcaaaggc cagcccgccg taaacaaaga cgtccccggt ctgccagata
     3301 tcagctcgca agccgcaatc cttgggctgc ttcggtcagc gccttgggct gatcgagcca
     3361 gatgccgcgg gtgtcataga ccgccttggt catgcgttcg gccagcggca cggccttgaa
     3421 ggcatcgtga tcgaccagca cgatcagcac gtcgcaggtc tccagcgccg tatcgacgtc
     3481 gatcagttcg gccgccgttc cgtcgaactc gcgcggcagc tcgctggcgt agggttcgac
     3541 caccttgatc cgactgccga acttgcgggc cagccgcgcc gcgaccgcgc gggccgggct
     3601 ttcgcggaaa tcgtcgatgt tggccttgaa ggccaggccc aggcaggcga cgcgggccgc
     3661 cgggtgagcc tcgatcaggg cttcagcgcg ggcgatcacg tggtggatct tgccgtcgtt
     3721 gacgccgcgg gcggtgcgga tcagcggggt ctcctcgggc gcgccgtgga cgatgaacca
     3781 ggggtccacc gcgatgcagt gcccgcccac gcccgggccc ggctgcagga tgttgacgcg
     3841 cgggtggcgg ttggccagcc ggatcacttc ccacacatcg aggcccatct tgtcggccac
     3901 gatgctgagc tcgttggcga aggcgatgtt gacgtcgcgg taggcgttct cgaccagctt
     3961 ggtcatttcc gcactgcggc tgtcggtgac cacgcattcg ccgcgcacga acagcttgta
     4021 gaagctgagc gccttgcggg cgcagcgcgg ggtgatcccg ccgatcgagc ggtcgttgtt
     4081 ggtcagttcc tccagaatgc ggccgggcag gacgcgttcc gggcaatagg aaatggcgat
     4141 gtccggcgtc tcgccggtca gaccggggca cttcaggtcc ggacggcgcg cggcgatcag
     4201 atcgcgcagc gcctcggtcg tgccgaccgg gctggtcgat tccaggatca cgcagtcgcc
     4261 ggccttcagc accttggcga ccgcttcgcc ggcggcgagg acataggaaa tgtcgggcgc
     4321 gtggttttca tcgaacgggg tcggcacggc gatgatgaag acgtcggccg gggccatctc
     4381 ggtcgaggcc ttgagcagcc cgcgcgtcac cacgccctgg accagcccgt cgagatcgac
     4441 ttcctcgatg tggatttcgc cgcggttgat cgtgtcgacc acgctttgcg tcacgtcgac
     4501 gccgtggacc ttgcagcccg cccgcgcgac cacggccgcg gtgggcagcc cgatgtaacc
     4561 aagcccgacg acacaaacgt ccggtttaac gtccgatttc attcccgatc agctccacga
     4621 tccggcgcgc ggagttcccg tccccgaagg gattgtgggc ccgggccata gcttcgtagg
     4681 ccgccttatc gtcgagaagg ttgaacaatt cggtaacgat aaccgcagtt tcggtgccga
     4741 ccagcttggc cgttccggcc gcgacgcctt cgggccgctc ggtcgtctcg cgcatcacca
     4801 gcaccggctt gcccagcgcg ggcgcttctt cctgcacgcc gccgctgtcg gtcagcatga
     4861 tctcggccag cgagagcagg tgggcgaagt gcggatagtc cagcggttcg atcagcgcga
     4921 cattgtccaa cccgtcgagc gccgcgccca tcaccttgcg gacgttgggg ttgaggtgga
     4981 ccggaaagat cacggccaca tcgggccgcg cggcgatctg gcggatcgcc tcggcaatcg
     5041 cttccatccc gccgccgaag ttttcgcggc ggtggctggt gacgccgatg atccgcttgt
     5101 cggcgaaccg cgccttcagc tcgtcgaggc cggcggccat ggccggattg gccgcgatcc
     5161 gggccgagac ccagtgcagc gcgtcgatca cggtgttgcc ggtcacgtgg acccgggccg
     5221 gatcgacctg ctcgtcggtc agcgccttgg ccgagacatc ggtcggcgcg aaatgcaggc
     5281 tggcgatggt gccgatgatc ttgcggttca cctcctccgg ccaggggtga tagatgttgc
     5341 cgctgcgcag gccggcctcg acatggtcga ccgggatctg gcggtaatag gcggcgagcg
     5401 cgccggtcat ggccgtggcg gtatcgccct ggacgatcac ccgggccggc ttcacctcgt
     5461 ccatcacccg gccgagaccc gtcagcagcg cggcggtcag gccgtccagc gtctggtctg
     5521 gcttcatcag gtccagatcg tgatcgggca ccagcccggc gatttccagc acctggtcca
     5581 gcatctggcg gtgctgggcc gaaacgcaga ccacgggggt gaagcggggg tcctcgcgca
     5641 gcgcggcgac gacggggaac agcttgatcg cttcggggcg ggttccgaag acgacgagga
     5701 tcttggcggg ctcggtcatg gcaaaaaccc cctaccagcg atgggttaac catgaaataa
     5761 ggccgcgcgg ctaggaccgc tccaaactat cggggaattt catgcgcgtt ctggttaccg
     5821 gcgttgccgg cttcatcggc tccaccctgg ccctgcgcct gctggaacgg ggcgacagcg
     5881 tggtcgggat cgactgcatc aacgactatt atcaggtctc gctgaaggaa gaccggctga
     5941 agaacgtcac cgccgcggcc ggcaaccgct ttgccttcca caaggtcgat ttcaccgaca
     6001 tggccgcgct ggacggcgcg ctgcagggca ccgaatttga ccggatcgtc cacctcggtg
     6061 cccaagccgg ggtgcgctac tccctcgaaa acccgcaggc ctatgtcagc gccaacctgg
     6121 ccgggcacgt caacctgctg gaaatcgccc gccaccgcca ggtggagcac atggtctatg
     6181 ccagctccag ctcggtctat ggcggcaaca ccaacatgcc cttctcggtc gaggaccggg
     6241 tcgaccatcc ggtctcgctc tacgccgcaa ccaagcgcgc ggacgagctg atgagcgaaa
     6301 cctatgccca ccagttccgc ctgccgctga ccggcctgcg cttcttcacc gtctatggtc
     6361 cctggggccg gcccgacatg atgatgtgga tcttcaccaa ggcgatcctg gccggccagc
     6421 cgatcccggt gttcaacaac ggcgagatgt accgcgactt cacctatatc gacgatatcg
     6481 ttgccggggt gatcaagtgc ctcgacagcc cgcccgccga tgacggcgcg gaaaaggccg
     6541 gcggcagcgt caagccgcac gcgctttaca acatcggcaa caacaagtcg gaacacctga
     6601 tgcgggtggt ggaactgctg gaggaaagct gcggcaagaa ggccgagatc gatttccagc
     6661 cgatccagcc gggcgatgtt cccgccacct atgccaacat cgacgcgatc cagcgcgacc
     6721 tgggctatgc gcccaccacc agcatcgatg tgggtgtccc caactttgtg cgctggtacc
     6781 gggactatca cggggtctga ccttgaagcc gaagccgccg cgcggcatgc tggccggatg
     6841 atcaggacat tcgcaatc
//
LOCUS       DASUDV010000076         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1813849,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000076 DASUDV010000000
VERSION     DASUDV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813849"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VFV30_01655"
     CDS             complement(<1..1121)
                     /locus_tag="VFV30_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX4846824.1"
                     /translation="MTFELSRRRFAAGMLGGAGLAFVPFAPALAAPADAPLPAGEDGK
                     LMALFREMAKREDALDPLGLVYRGGKANVTAFRQIFTDAPLRARRGLVNWCLNSLRRI
                     DRAKLSPERQISYDVFLRDKRETQMWLQPDMLALIVVRPFNHFGGIHMEFPSLFAVGG
                     VLGYADEDNYRRNLELLRAFPQVLDNAIARFRQGLDSGVVETALTTRNMVGQIDSLLA
                     QPLDESPFTAGLRDFPASVPPPRRDGLRQAYLTVLNREVLPAYRKLRAFLNDEYLPAA
                     RQQVGIGAMKGGPGLYRRLIESQTTVRLEPEDVHQLGLAEVARIQRDMDGVRQKLGFA
                     GPLRAFFDEIRTNPRYHPKTKEEFAKAFADAAKAVDAQVP"
     gene            complement(1121..1798)
                     /locus_tag="VFV30_01660"
     CDS             complement(1121..1798)
                     /locus_tag="VFV30_01660"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX4846825.1"
                     /translation="MSPFSEAPAPDKQALIKVGEHVRQRLDAEPRAVRIDTDKADIWA
                     VGDMFDAQECRQLIAMIDRTAVPSQVLTHGEIEVWRTSSSGDVDPFDPFIQRIEERID
                     ALLDLPHEWGETMQGQRYDVGQEFKYHLDSFWTKADYWQDEAKRGGQRTITAMAYLNN
                     VEEGGDTLFTTLGLSIKPQRGALLIWNNNLPTGLPNEFTMHAGTPVVKGVKYVLTKWY
                     RSRRWGY"
     gene            1930..>2351
                     /locus_tag="VFV30_01665"
     CDS             1930..>2351
                     /locus_tag="VFV30_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX4846826.1"
                     /translation="MDTAASSAARYSRGAIALHWIIALLIVLNFVVAWLAEDAPKEDA
                     AVMMGNHKAIGITILALTVARIVWRLTHKAPPLLESLKAWEAALAKVTHGLFYLLMIA
                     IPVAGWGLASAYGKGKPVSMFGLFDVPALPVGHDKATVG"
BASE COUNT          373 a          782 c          787 g          409 t
ORIGIN      
        1 ggcacttgcg cgtcgaccgc cttggccgca tcggcaaagg ccttggcgaa ttcctctttg
       61 gtcttcgggt gataacgcgg attggtgcga atctcgtcga agaaggcccg caaggggccc
      121 gcaaagccga gcttctggcg cacgccgtcc atgtcgcgct ggatccgggc gacttcggcc
      181 aggcccagct ggtggacgtc ctccggctcc agccggacag tggtctggct ttcgatcagc
      241 cggcggtaga gcccgggccc gcccttcatt gccccgatcc cgacctgctg gcgcgcagcc
      301 ggcagatact cgtcgttcag gaaggcgcgc agcttgcggt aggcgggcag gacctcgcgg
      361 ttcagcacgg tcagataggc ctggcgcagg ccgtcgcgcc ggggcggcgg cacgctggcg
      421 ggaaagtccc gcaggccggc ggtgaagggg gattcgtcca gcggctgggc cagcaggctg
      481 tcgatctggc caaccatgtt gcgggtggtc agcgcggttt ccaccacgcc gctgtccagc
      541 ccctggcgga accgggcaat cgcgttgtcg agcacttgcg gaaaggcgcg cagcagctcc
      601 aggttgcggc ggtaattgtc ctcgtcggca tagcccagca cgccgccaac cgcgaacagc
      661 gaggggaatt ccatgtggat cccgccgaag tggttgaacg ggcggaccac gatcagcgcc
      721 agcatatcgg gctgcagcca catctgcgtc tcgcgcttgt cgcgcaggaa cacgtcatag
      781 gaaatctgcc gctcggggct cagcttggcg cggtcgatcc ggcgcaggct gttcaggcac
      841 cagttgacca ggccgcgccg cgcgcgcagc ggggcatcgg tgaagatctg gcgaaaggcc
      901 gtcacgttgg ccttgccgcc gcggtagacc aggcccagcg gatcgagcgc gtcctcgcgc
      961 tttgccatct cgcggaacag cgccatcagc ttgccgtcct cgcccgcggg caggggggca
     1021 tcggcagggg ccgccagcgc cggcgcgaag ggcacaaagg ccagcccggc gccgcccagc
     1081 atcccggcgg caaagcgccg ccggctcagt tcaaatgtca tcaatacccc catcggcgcg
     1141 accggtacca tttggtgagg acgtatttca cgcccttcac caccggggtc ccggcgtgca
     1201 tcgtaaactc gttcggcagc ccagtgggca ggttgttgtt ccagatcagc agcgccccgc
     1261 gctgcggctt tatcgacagg cccagcgtgg tgaacagggt gtccccgcct tcctcgacat
     1321 tgttgagata ggccatggcc gtgatcgtcc gctgtccgcc gcgcttggcc tcgtcctgcc
     1381 agtagtccgc cttggtccag aaactgtcga ggtggtactt gaactcctgg cccacgtcat
     1441 agcgctggcc ctgcatcgtc tcgccccatt cgtgcggcag gtcgagcagg gcgtcgatcc
     1501 gctcctcgat ccgctggatg aacggatcga acggatcgac atcgcccgag ctgctggtcc
     1561 gccagacctc gatctcgccg tgggtgagga cctgcgaggg caccgcagtg cgatcgatca
     1621 tcgcgatcag ctggcggcat tcctgggcat cgaacatgtc gcccacggcc cagatgtcgg
     1681 ccttgtcggt atcgatccgc acggcgcgcg gttcggcgtc aagccgctgg cggacatgct
     1741 cgccaacctt gatcagggcc tgcttgtcgg gcgccggggc ttcgctgaac ggactcatca
     1801 acggttgcct aaccgatcgc ggcgaaaggg caaatgggca atcgcatggc gttgatcgat
     1861 caatcccgcc ggttcgccgc cgcgatcaac ccacttgccc gaacggcgca aatcggccag
     1921 cttggcgcga tggataccgc agcctcttcc gccgcccgct attcgcgcgg cgccatcgcc
     1981 ctgcactgga tcatcgccct gctgatcgtg ctcaatttcg tcgttgcctg gttggccgaa
     2041 gatgcgccca aggaggacgc ggcggtcatg atgggcaacc acaaggcgat cgggatcacc
     2101 atcctggcgc tgaccgtggc ccggatcgtc tggcgcctga cccacaaggc cccgccgctg
     2161 ctggaatcgc tgaaggcctg ggaagcggcg ctggccaagg tgacccatgg attgttctac
     2221 ctgctgatga tcgcgatccc ggtggcgggc tgggggctgg cctcggccta tggcaagggc
     2281 aagccggttt cgatgttcgg cctgttcgat gtgccggccc tgccggtcgg ccacgacaag
     2341 gcgacggtcg g
//
LOCUS       DASUDV010000077         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_753570,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000077 DASUDV010000000
VERSION     DASUDV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753570"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFV30_01670"
     CDS             complement(<1..306)
                     /locus_tag="VFV30_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4846827.1"
                     /translation="MNDAVKAAWMGLQGLEQHSLSALFADPGRLDLLSSRLDLPGGAI
                     RFDWSKTHLDAAHLAAFEALAEAVGFRRQRAAMFAGEPINVTEGRAVEHTALRGVGRE
                     "
     gene            complement(662..1000)
                     /locus_tag="VFV30_01675"
     CDS             complement(662..1000)
                     /locus_tag="VFV30_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX4846828.1"
                     /translation="MKFIIAIIKPFKLDEVREALSALGVSGMTVTEVKGFGRQKGQTE
                     ISRGAEYTTTMLPKLKVELAVSDDLAPQVIETIQHQASTDSIGDGKIFVLDLLAATRI
                     RTGETGENAL"
     gene            complement(1155..1877)
                     /locus_tag="VFV30_01680"
     CDS             complement(1155..1877)
                     /locus_tag="VFV30_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4846829.1"
                     /translation="MIAAHPVELTFATYNIRKAVGLDRRRDPERILSILNEIDADVVA
                     LQEVDRRIGRRASVLPLDHIEDYTPYQPVPLNHRPDSLGWHGNALLVRRGIKLAEASI
                     VPLPVLEPRGAIRADLLVEGRRIRVVGMHLDLSGLRRRHQLRAILAHLERCDHHCPTV
                     AMGDCNEWGMRRGALSEFAEPWRVLAPGFSFPTRRPVARLDRIAISREWQVEQAAVHH
                     SPLAARGSDHLPVWARLKLPKK"
     gene            complement(1874..>2019)
                     /locus_tag="VFV30_01685"
     CDS             complement(1874..>2019)
                     /locus_tag="VFV30_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4846830.1"
                     /translation="QLERARETLVKYHAQADPAKWEAKVAGRTRSIDGLPTRPTDRYI
                     AGE"
BASE COUNT          323 a          668 c          670 g          358 t
ORIGIN      
        1 ttcgcgcccc accccgcgca gcgcggtgtg ttcgaccgcg cgtccttcgg tgacgttgat
       61 cggctcgccc gcaaacatcg cagcccgctg gcggcgaaag cccaccgcct cggccagcgc
      121 ctcgaacgct gccaggtgcg cggcgtcgag atgggtcttc gaccagtcga agcggatcgc
      181 cccgcccggc agatcgagcc tgctcgacag caggtcgagc cgccccggat cggcgaacag
      241 cgcgctcagg ctatgctgtt caagcccctg taggcccatc caggcggctt ttacggcgtc
      301 gttcatggcc atccttccgt tgtttggtcg aaaggcgcta tgcctgtaga gccggcccgg
      361 gccaagccgg gaaaacgtat tcaggcggcg tgccgagagc cagatgacgt taggttgcat
      421 cgccgggatt gcgtcaggaa gcttctcggc aaggagcgtt gcgcaggccg ggctggttgc
      481 ccggaccagc aacgcgacgc agtccgaggg gcttcctgac gcaaccgcta gcggcgggcc
      541 gattttggcc catcgcagcg tcaatcgtcg gtcactatgc aatggcatgg ctccctcctc
      601 tttcctcgcg ctgagccaaa atcggctccg tcacgacgat gcagcctgac gtcatcaggg
      661 tctaaagcgc attctcgccg gtttcgccgg tccggatccg ggtcgcggcc agcagatcga
      721 gcacgaagat cttgccgtcc ccgatcgaat ccgtgctggc ctggtgctgg atggtttcga
      781 tcacctgcgg cgcgagatcg tcgctgaccg ccagttccac cttgagcttg ggcagcatgg
      841 tggtggtgta ttcggcgccc cgggagatct cggtttgccc cttctgccgc ccgaagccct
      901 tgacctcggt aacggtcatg cccgacacgc ccagcgccga aagcgcctcg cgcacctcgt
      961 caagcttgaa cggcttgata atggcgatga tgaatttcat gccccggttg cctcgatttt
     1021 cagccggcgg aaatgccggc ccaggcagtt cagcaagggc tatgccaatt tgacaaaacg
     1081 cttcgttttc aatcggcgag gtgcaagggg gcggcacggc cgcaagcgtg ggcgcctcaa
     1141 ttcggcgcag ctgcctattt tttgggcagc ttgagcctgg cccagaccgg gagatgatcc
     1201 gagccccggg cggccagcgg cgaatggtga acggctgcct gctcgacctg ccattcgcgg
     1261 ctgatcgcga tccggtcgag ccgggcgacc gggcggcgcg tggggaagct gaagcccggg
     1321 gcgagcacgc gccagggctc ggcaaattcg ctcagcgcgc cgcgccgcat gccccattcg
     1381 ttgcaatcgc ccatcgccac ggtcgggcaa tggtggtcgc accgttcgag gtgggcgagg
     1441 atcgcgcgca gctggtggcg ccgccgcagg cccgacagat cgaggtgcat gccgacaacc
     1501 cggatgcgcc gcccctctac cagcagatcg gcccggattg cgccgcgcgg ctcgagcacc
     1561 gggagcggca cgatcgaggc ttcggccagt ttgatcccgc gccgcaccag cagggcattg
     1621 ccgtgccagc ccagcgaatc cgggcgatgg ttgagcggca ccggctggta gggggtatag
     1681 tcctcgatgt gatcgagcgg cagcacgctg gcgcgccggc cgatccgccg atcgacctcc
     1741 tgcagcgcca ccacgtcggc atcgatctcg ttcaggatcg agagaatccg ttcgggatcg
     1801 cggcgccggt caagcccgac agccttgcgg atattgtagg tggcgaaggt cagttccacg
     1861 ggatgcgccg cgatcattcc ccggcgatgt agcggtcggt cggccgcgtc ggcagaccat
     1921 cgatgctgcg ggtccgcccg gcaacctttg cttcccattt cgccggatcg gcctgggcat
     1981 ggtacttgac cagcgtttcg cgggcgcgct ctagctgca
//
LOCUS       DASUDV010000078         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_24705,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000078 DASUDV010000000
VERSION     DASUDV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24705"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFV30_01690"
     CDS             complement(<1..727)
                     /locus_tag="VFV30_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4846831.1"
                     /translation="MRAARLLSAAAALALALNGAGPGLAKDKKKEESAAAKAAREFNK
                     DPYPSTYRPYANAPTLIVGATVFDGAGKRYDGASVELRDGKIAAIHQGKVEAPAGTTV
                     IDATGKFVTPGIIDAHSHLGDYPSPGVDAHSDGNEATEPTTPNVWAEHSVWPQDPGFS
                     RAMANGGVTALMVLPGSANLMGGRGVVLKNVPSRTVQGMKFPAAPYSLKMACGENPKR
                     VYGGKGRMPSTRMGNFAVNRATWL"
     gene            complement(727..>2035)
                     /locus_tag="VFV30_01695"
     CDS             complement(727..>2035)
                     /locus_tag="VFV30_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX4846832.1"
                     /translation="AADGSEARKVAGDRFKADGERSPFNVFLLLVGLAMVTVGNGFFK
                     PNISTMVGSLYKQGDPRRDSGFTIFYMGINLGSLLSQFLCPLLAVGMGSWQGIGWWAG
                     FLLASLGMMCSWALIQFDGGRLGGIGERPADAPANKDLLIYAGALAAVPVVWLLSSNL
                     MTYVAPAEGTGLIGYFMGLPIMGKLMFGTFLFAVPAILYWAWRHGNKVEFQMMLAAMV
                     LITFNTVFWTLFEQAGSSLTLFAERNTNLSVFGLFEINAAQTQIFNALFIVLLAPVFS
                     ALWIKLAKRGLEPSIPVKFGVALMGVGGGFLFLVFGAQFADGAFQVGLWWLAGLYLIH
                     SMAELLISPVGLSMITKLSIARIVGLMMGVWFLSIAVAQYVAGVVAQFASVETVGGQV
                     TNLKVSLDTYVGVFTTIGWVSIIIGGVLLVLALPLKKLMHGVK"
BASE COUNT          386 a          680 c          651 g          318 t
ORIGIN      
        1 tcagccaggt agcgcggttg accgcgaaat tgcccatccg ggtcgatggc atccggccct
       61 tgccgccata gacgcgcttg gggttttccc cgcaggccat cttgaggctg tagggcgcgg
      121 ccggaaactt cataccctgg accgtgcggc tgggcacgtt cttgagcact accccgcgcc
      181 cgcccatcag gttggccgat cccggcagca ccatcaaggc ggtcaccccg ccgttggcca
      241 tcgcgcgcga aaagcccggg tcctgcggcc agaccgaatg ctcggcccag acgttgggcg
      301 tggtcggttc ggtggcttcg ttaccgtcgg agtgggcatc gacgccgggc gatgggtaat
      361 cgcccaggtg gctgtgcgca tcgatgatcc ccggggtcac gaacttgccg gttgcatcaa
      421 tcaccgtagt gccggcggga gcctcgacct tgccctggtg gatcgcggcg atcttgccat
      481 cgcgcagttc aacactggcg ccgtcatagc gcttgcccgc cccgtcgaac accgtggccc
      541 cgacgatcag cgtcggggca ttggcatagg gccggtaggt cgaaggatag ggatccttgt
      601 tgaactcccg cgccgccttg gccgcggcac tttcttcctt cttcttgtcc ttggccaggc
      661 ccggcccggc cccgttgagg gcaagggcca gggccgcggc ggcgctcagc agccgggcag
      721 cgcgcatcac ttcactccgt gcatcagttt cttgagcggc agcgcgagca ccagcagcac
      781 gccgccgatg atgatcgaga cccagccgat cgtggtgaac acgccgacat aggtgtcgag
      841 gctgaccttg aggttggtga cctggccgcc gaccgtttcg accgaggcga actgggccac
      901 gaccccggcg acatactgcg ccaccgcgat cgacaggaac cagacgccca tcatcagccc
      961 gacgatcctg gcgatcgaca gcttggtgat catcgataga cccaccggcg agatcagcag
     1021 ttcggccatc gaatggatca ggtagagccc ggccagccac cacagcccga cctggaaggc
     1081 gccatcggca aactgtgcgc cgaacaccag gaacaggaag ccgccgccca cgcccatcag
     1141 cgccaccccg aacttcaccg ggatcgacgg ttcaagcccg cgcttggcca gcttgatcca
     1201 cagcgcgctg aataccgggg ccagcagcac gatgaacagc gcgttgaaga tctgggtctg
     1261 cgcggcgttg atctcgaaca gaccgaagac cgaaaggttg gtgttgcgtt cggcgaacag
     1321 cgtgagcgag gagccggcct gctcgaacag ggtccagaac accgtgttga aggtgatcag
     1381 caccatcgcc gccagcatca tctggaactc caccttgttg ccgtggcgcc aggcccagta
     1441 caggatcgcc gggaccgcaa acaggaaggt gccgaacatc agcttgccca tgatgggcag
     1501 gcccatgaag tagccgatca ggcccgttcc ttcggccggc gcgacatagg tcatcaggtt
     1561 ggaggacagc agccagacta ccggcaccgc cgccagcgcg ccggcgtaga tcagcagatc
     1621 cttgttggcg ggcgcgtcgg ccggacgttc gccgatgccg ccaagacggc cgccgtcgaa
     1681 ctggatcagc gcccacgagc acatcatgcc gagcgaggcg agcaggaagc cggcccacca
     1741 gccgatgccc tgccacgagc ccatgcccac cgcgagcagc gggcagagga actgcgacag
     1801 cagcgaacca aggttgatcc ccatgtagaa gatcgtgaac ccggaatcgc ggcgggggtc
     1861 gccctgcttg taaagcgagc cgaccatggt cgagatgttg ggcttgaaga atccgttgcc
     1921 gaccgtgacc atcgccagac cgaccagaag caggaagacg ttgaacggcg agcgttcgcc
     1981 atcggccttg aaccggtcgc ccgcgacctt gcgcgcttcg ctgccatcgg cggcc
//
LOCUS       DASUDV010000079         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_731490,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000079 DASUDV010000000
VERSION     DASUDV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731490"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFV30_01700"
     CDS             complement(<1..233)
                     /locus_tag="VFV30_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4846833.1"
                     /translation="MRQIALWLCAVLASLTLAVSGTAALAELPPRPDGPVLDAAGIIP
                     DADEAALDAKLRAYTKDTGRAIIVATVPSLDDQP"
     gene            complement(263..943)
                     /locus_tag="VFV30_01705"
     CDS             complement(263..943)
                     /locus_tag="VFV30_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4846834.1"
                     /translation="MTAAIVRRFGRLALVAAAAAALAGCGINSVPTAEENAKAKWADV
                     ESAYQRRADLIPALVETTKGAAASETQILTNVTNARAAATSINVRTEDLSDPATFEKF
                     QNAQNQLTQALGQLRTVVENYPQLQSQARFADLMVQLEGTENRINVARTRYNEAVQEY
                     NTTIRTFPDAIGAKLIHGAKPMVAFKADPGASVAPKVDFGTAQAPAAAAPANDNAAAP
                     ASPAAAAQ"
     gene            1123..1530
                     /gene="mscL"
                     /locus_tag="VFV30_01710"
     CDS             1123..1530
                     /gene="mscL"
                     /locus_tag="VFV30_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX4846835.1"
                     /translation="MLSEFKAFIARGNVLDLAVGVIIGAAFGKIVSSLTDDVIMPVIG
                     FITGGIDFSNKYTVLAGEAPAGATLAAAKAAGATVIAWGSFVTAVINFLILAFVIFLI
                     VRQANKLMPPPPAATGPSEVDLLTEIRDALKKG"
     gene            1574..1922
                     /gene="ssrA"
                     /locus_tag="VFV30_01715"
     tmRNA           1574..1922
                     /gene="ssrA"
                     /locus_tag="VFV30_01715"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT          353 a          642 c          722 g          407 t
ORIGIN      
        1 ggctggtcat cgagcgaggg gactgtggcg acgatgatcg cccgcccggt gtccttggtg
       61 taggcgcgca acttggcatc cagcgccgct tcatcggcat cgggaatgat cccggctgca
      121 tcaagcaccg gcccatccgg acgaggtggc agttctgcca gggcggccgt gccggacacc
      181 gccagggtca gcgatgccag cacggcgcaa agccacaggg cgatctggcg catggcgccg
      241 ggctccgtct ggaggtgccg gcttactgcg ccgccgcagc gggcgaagcc ggagccgccg
      301 cgttatcatt ggccggggca gccgcagccg gcgcctgggc ggtgccgaaa tcgaccttgg
      361 gtgcgaccga ggcgcccgga tcggccttga acgccaccat cggcttggcg ccgtggatca
      421 gcttggcacc gatcgcgtcg gggaaggtgc ggatcgtggt gttatactcc tgcaccgcct
      481 cgttgtagcg ggtgcgggcc acgttgatcc ggttttccgt cccttccagc tgaaccatca
      541 gatcggcgaa acgcgcctgg ctctgcagct gcggatagtt ttccaccacg gtgcgcagct
      601 ggcccagtgc ctgggtcagc tggttctgcg cgttctggaa cttctcgaag gtggcggggt
      661 ccgaaaggtc ctcggtccgc acgttgatcg aagtcgcggc agcgcgcgcg ttggtgacat
      721 tggtcaggat ctgggtttca gaggcggcgg cgcccttggt ggtctccacc agcgcgggga
      781 tcaggtcggc acggcgctga taggcgcttt cgacatcggc ccacttggcc ttggcgtttt
      841 cctcggcggt cggcaccgaa ttgatcccgc agccggccag cgcggcagca gcagcggcga
      901 ccagggcaag gcggccgaaa cgacggacaa tggcagcggt catgcaaggc atccctattc
      961 caaggcggcc cacccggccg cgactgtgca tggatgtagc gcggggggcg gcgggttcaa
     1021 gcggtgctcg caaaaatcgt tgcagtgcaa ttgattgttg ttgccgccgg gctttcgtgg
     1081 caggttttcc gcactcgtga aaatgagtga aggggacgca aaatgcttag tgaattcaaa
     1141 gcctttatcg cgcgcggcaa cgtgctggac ctggcggtcg gtgtgatcat cggcgccgct
     1201 ttcggcaaga tcgtttcgtc gctgaccgat gacgtgatca tgccggtgat cggcttcatc
     1261 accggcggga tcgatttctc gaacaaatac accgttctgg cgggggaagc gcctgccggc
     1321 gcgacgctgg ctgccgccaa ggccgccggg gccaccgtga ttgcctgggg cagctttgtc
     1381 actgccgtga tcaacttcct gatcctggcc ttcgtgatct ttctgatcgt gcgccaggcc
     1441 aacaagctga tgccgccgcc gcccgctgca accgggccga gcgaggtgga cttgctgacc
     1501 gagatccggg acgcgctcaa gaagggctga tttgcgatca gctgcgacgc gaaagggcgg
     1561 gggccgggct cccgcccttt tcatttgggc agggcttccc tatatgggtt cttgccgggt
     1621 tcgcccggct atggcgataa actgcggcgt gcaataggcg cagcggaccc gggggcggta
     1681 cccggcggct ccaccaccaa tcctgttgtc gcaggggtga tgacggggcc gaactaggat
     1741 cgacgtgtgt tgaaaagcgc tgttttcgcc cgggctgagt aacccgttca aggctcaaaa
     1801 ctcacaagtg ccaacgataa cgaagcactt gctcttgctg cgtaattcta gggcctagcg
     1861 gcctgaagtt acactaccaa gaacggggtc cggggcgtac cttgcaacag aaacgcccca
     1921 cttccttcat gagggtgcgg tcggtccatc cgggccgacc ttgcattacc agcccgcgcc
     1981 cccgggccag ccactcgatt aggataccct tggggcggtt gggccggggg cgcgggctgg
     2041 ttatgcaaag ctatcgacgg atgtcgatag cgcaccaggc taggattctt cccctaggcg
     2101 cttttcgtgc ttcaggcaat cgag
//
LOCUS       DASUDV010000080         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_157248,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000080 DASUDV010000000
VERSION     DASUDV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157248"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFV30_01720"
     CDS             complement(<1..306)
                     /locus_tag="VFV30_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX4846836.1"
                     /translation="MLSSIRRTLREIKTSTSGNATLLVALGMPVLIGGSGLAVDTAQW
                     YMWKREIQYAADQAALAGAWARSSEDSRSNYVTRARQEFGANISATTGINSTPNVSLA
                     "
     gene            461..1117
                     /locus_tag="VFV30_01725"
     CDS             461..1117
                     /locus_tag="VFV30_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846837.1"
                     /translation="MNPQALLDELPIERRLAMAYAPAAARPLTLGLFALDTRLAGIVR
                     QASEPLLGQIRLAWWREQLAADPASTPRGEPLLALLADWGARRAALSSLVDGWEALLG
                     GDMLLEEGLERFVHARGAAAALLAQALEQDGVSAEAEQAGRAWAAAELAVRLSDPAER
                     AMALRMAGAADWGRIKLPRALRPLAVLHGLARRKKGQGALLSGPVDMLVAARLGLTGF
                     "
     gene            1150..1626
                     /locus_tag="VFV30_01730"
     CDS             1150..1626
                     /locus_tag="VFV30_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846838.1"
                     /translation="MNRMVLGAVSALLLAAAGLFWWQGQATTDSSAPPAPPIPAEAVA
                     AGPELDEIPTEDGDGLQGPALPGVSEATREEKRFNRLDRNRDNLISRNEALQPRVAAF
                     RKLDTDGNNLLSFEEWAVTTTNRFKKADGNGDRQLTRQEFATTKPKQQAKKPDCRC"
     gene            complement(1681..2445)
                     /locus_tag="VFV30_01735"
     CDS             complement(1681..2445)
                     /locus_tag="VFV30_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX4846839.1"
                     /translation="MRNLVYTAAVAACCLATPAFAEDGDWSGAYIGISAGYTAAKSDS
                     TAVIGGSWTSESVALQNEVTSRLAQDQSFDDVNFGGQIGYNYQTGGAVMGIEFDMSAL
                     SGSEVLVRGPIATTAFPALSYTYTNRIDPKHMFSIKGKLGAAMGNTLLYVEGGWAWTK
                     AELGADLSSNGGYSKSGRLSKTMDGFIVGGGIEQRFGGNVSVRLSYHYTDQGSESYAT
                     AYNAGSSFAPPGNNYTEVMTQDLRLHLVRVGLNFHF"
     gene            complement(2560..>2895)
                     /locus_tag="VFV30_01740"
     CDS             complement(2560..>2895)
                     /locus_tag="VFV30_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4846840.1"
                     /translation="LRLKRAPHIRFAGQITGCEGYVESAAVGLIAGLMTAAELGGRPW
                     QSPPRTTALGALLSHITGDADAETYQPMNINFGLFPPPDAEVKKKERKQAYTARAKRD
                     LADWLPAAG"
BASE COUNT          464 a          920 c         1003 g          508 t
ORIGIN      
        1 ggcgagcgag acgtttggcg tggagttgat gccggtggtt gccgagatgt tggcaccgaa
       61 ctcctgccgg gcccgcgtga catagttcga gcgcgagtcc tcgctgctgc gggcccaggc
      121 gccggccagg gcggcctggt cggcggcgta ctggatttcg cgtttccaca tgtaccactg
      181 ggcggtgtcg accgcgaggc cggagccccc gatcagaacg ggcatgccaa gcgccacgag
      241 cagcgtggcg tttccgctgg tgctggtctt gatctcgcgc agagttcggc gaatcgagct
      301 gagcatgatg cccccaaaaa tgaatccaag tgatctgcgc ttagaccctt gtggtgactg
      361 tactttcaaa cagcagggtt aacagcgggt taggatgcgc cggaaatccg cttctttccc
      421 gaagacttgc gcgcggcggg cggaacgcta gggcggggcg atgaacccgc aagcccttct
      481 ggatgaactg ccgatcgagc ggcgcctggc aatggcctat gccccggctg cggcccgccc
      541 gttgaccctg gggctgttcg cgctcgacac ccggctcgcc ggcattgtcc gccaggcgag
      601 cgagccgctg ctggggcaga tccggctcgc ctggtggcgc gagcagctgg ccgccgatcc
      661 cgccagcacc ccgcgcggcg agccgctgtt ggcgctgctg gcggactggg gtgcccggcg
      721 cgccgccttg tccagcctgg tcgatggctg ggaagcgctg ctgggcgggg acatgctgct
      781 ggaagagggg ctggagcgct ttgtccacgc ccgcggcgcg gcggcggccc tgcttgcgca
      841 agcgcttgaa caggatgggg tttccgccga agccgagcag gccggacggg cctgggccgc
      901 ggccgaactg gccgtccggc tgagcgatcc ggccgaacgg gccatggccc tgcggatggc
      961 gggtgcggcc gattggggcc ggatcaaatt gccgcgcgcg ctgcgcccgc ttgcagtctt
     1021 gcacgggctg gcacggcgca agaaagggca gggggcgttg ctgtccgggc cggtcgatat
     1081 gctggttgcg gcaagacttg gcttgaccgg tttctaggct attgtcggcc tctatttgga
     1141 ggctcgatca tgaatcggat ggtgctgggt gccgtttctg cgctgttgct tgccgcggcg
     1201 gggttgttct ggtggcaggg gcaggcaacc accgattctt cggcaccgcc cgcaccgccg
     1261 atcccggccg aggcagtggc cgccggtccg gaactggacg agatcccgac cgaggacggc
     1321 gacggcctgc aaggcccggc gctgcccggc gtgagcgagg ccacgcgtga ggaaaagcgc
     1381 ttcaaccggc tcgaccgcaa ccgtgacaac ctgatcagcc ggaacgaggc gctgcagccc
     1441 cgcgtcgcgg cgttccgcaa gctcgacacc gacggcaaca acctgttgag tttcgaggag
     1501 tgggccgtca ccaccaccaa ccgcttcaag aaggccgatg gcaacggcga ccggcagctg
     1561 acccgccagg aattcgccac gaccaagccc aagcagcagg ccaagaagcc ggactgccgg
     1621 tgctgaaacg cccgcccggc ggttgccgga cgggcgctgt gggcacggct gtgcgccgga
     1681 tcagaagtgg aagttcagac cgacccggac cagatgcaga cgcaggtcct gagtcatgac
     1741 ttcggtgtaa ttgttacccg gcggggcaaa ggagctgccc gcgttgtaag cggtggcata
     1801 gctttcgctg ccctggtcgg tgtagtgata gctcagccgg accgagacgt tgccaccaaa
     1861 gcgctgctcg atcccgccgc cgacaatgaa cccgtccatc gtcttcgaca gccgtccgga
     1921 cttgctgtag ccgccgttcg acgagagatc ggcccccagt tcggccttgg tccaggccca
     1981 gccgccttcg acgtagagca gcgtgttgcc catcgccgcg cccagcttgc ccttgatcga
     2041 gaacatgtgc ttgggatcga tgcggttggt gtaggtgtag ctcagcgccg gaaaggccgt
     2101 cgtcgcgatg ggtccgcgga ccagcacttc gctgccgctg agcgcgctca tgtcgaactc
     2161 gatgcccatc acggcgccgc cggtctgata gttgtagccg atctggccgc cgaagttcac
     2221 gtcatcgaag gactggtcct gggcaagccg gctggtgact tcgttctgca gagcaaccga
     2281 ttcggacgtc cagcttccgc cgatcacggc ggtgctgtcg gacttggcgg cggtgtagcc
     2341 ggcgctgatc ccgatgtagg cgccgctcca atcgccgtct tcggcgaagg ctggcgtggc
     2401 aaggcagcag gccgcaacgg cggccgtgta gacgaggttt cgcattttcc cctccctagt
     2461 ggtcaaacat aagcgacccg gtgagtgtca aatacgcctg ccaaacggcg gaaaattgta
     2521 ggaactcgcc tgaaaactgg ttgcaatcga ttctattttt catcccgccg ccggcagcca
     2581 atcggcgagg tcgcgcttgg cccgggcggt gtaggcctgc ttgcgctcct tcttcttcac
     2641 ttcggcgtcg ggcggtggaa acagcccgaa attgatattc atcggctgat aggtctcggc
     2701 gtcggcatcg ccggtgatgt gcgacagcag cgcccccagc gccgtcgtgc gcggcggcga
     2761 ttgccagggc cgtccgccca gttcggcggc tgtcatcaga cccgcgatca ggcccaccgc
     2821 ggcgctttcg acatagccct cgcagccggt gatctggccg gcaaagcgga tgtgcggggc
     2881 ccgcttcagc cgcag
//
LOCUS       DASUDV010000081         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_996676,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000081 DASUDV010000000
VERSION     DASUDV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996676"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VFV30_01745"
     CDS             complement(<1..1299)
                     /locus_tag="VFV30_01745"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX4846841.1"
                     /translation="MTHSYDADLAMFIAGSWRLGEGRDTFAVVDPARGEAIAEVPLAS
                     PADLEEALDAADKGFALWRATPVEQRAAVLKKAAALIRERAEAIAQLLTLEQGKPIAE
                     ARGEVMSSAQLFDWCAEEASRIYGRVLVRPTGQRGLVIKQPIGPVAALSPWNFPIYLM
                     AKKLAPALAVGCSVIAKPPEETPGCTGALMRCLADAGVPAGVAQLVHGVPDTVSRTLI
                     GSPVIRKVSFTGSIPVGKHLMKLAADGLKRVTMELGGHAPVLVFEDCDLEKTLDMVVP
                     QKFRNAGQVCVSPTRFYVQESIYQRFIDGFAARTAGVKTGHGLDADTRMGPLANVRRP
                     QAIAALVEDAASKGARVMAGGKRGNEGFFFQPTLLADVPDNADIMNNEPFGPVAVAAP
                     FSDLADALGKANRLPYGLAAFAFTESLRTANLLGDGIESGM"
     gene            complement(1365..1802)
                     /locus_tag="VFV30_01750"
     CDS             complement(1365..1802)
                     /locus_tag="VFV30_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4846842.1"
                     /translation="MSSGDLLDRIEGAGAFDDGKDPEALRTIGEVAKALGIRQHVLRY
                     WEEQFPMLKPLKRSGGRRYYRPEDVVLVETIDRLVHREGYTLKGAKKALQGGAAKAAA
                     AVAAPSAPAPAAVQPSTPLPNAFAYRARLIALRTTLVNALHEA"
     gene            complement(1799..2086)
                     /locus_tag="VFV30_01755"
     CDS             complement(1799..2086)
                     /locus_tag="VFV30_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX4846843.1"
                     /translation="MSTLTRADLAEAINRKLGLSRSESLTMVEAILDHMSNALESGEN
                     VKISGFGTFLLRDKGQRVGRNPKTGIEVPITPRRVLTFRASQMLKERIVRG"
BASE COUNT          350 a          694 c          667 g          375 t
ORIGIN      
        1 catcccgctt tcgattccgt cgcccagcag gttcgcggta cgcaggcttt cggtgaaagc
       61 aaaggccgcc agaccataag gcaggcggtt ggccttgccc agcgcatcgg ccagatcgct
      121 gaacggggcg gccacggcca ccgggccgaa gggctcattg ttcatgatgt cggcattgtc
      181 gggaacatcg gccagcagcg tcggctggaa gaagaagccc tcattgcccc gcttgccccc
      241 ggccatgacc ctggcgccct tgctcgccgc atcctcgacc agcgcagcga tcgcctgcgg
      301 acggcggaca ttggcaagcg gccccatcct ggtatcggca tccagcccgt gccctgtctt
      361 gaccccggca gtacgcgcgg caaagccatc gatgaagcgc tggtagatcg attcctggac
      421 gtagaaccgg gtcgggctga cgcagacctg cccggcgttg cggaactttt gcggcaccac
      481 catgtcgagc gtcttttcca gatcgcaatc ctcgaacacc agcaccgggg cgtgtccgcc
      541 cagttccatg gtcacccgct tgaggccgtc agcagcaagc ttcatcaagt gcttgcccac
      601 cggaatcgag ccggtgaagc tgaccttgcg gatcaccggc gagccgatca gcgtgcggct
      661 gacggtatcg ggcacgccgt gaaccagctg cgccacgccc gcgggaactc cggcatcggc
      721 gagacagcgc atcagcgcgc cggtgcagcc cggggtctcc tcgggtggct tggcgatcac
      781 cgaacagccc acggccagcg ccggggccaa cttcttggcc atcaggtaga tcgggaagtt
      841 ccaagggctg agtgcggcaa ccgggccgat cggctgcttg ataaccagtc cgcgctggcc
      901 ggttgggcga accagcacgc ggccataaat gcggctggct tcctcggcac accagtcgaa
      961 cagctgggca ctgctcatca cttcgccgcg cgcctcggca atcggcttgc cctgttcgag
     1021 ggtcagcaac tgggcgatgg cttcggcgcg ttcgcggatc agagcggccg ccttcttgag
     1081 caccgctgcg cgttgttcaa ccggggttgc ccgccacagc gcaaagccct tgtcggcggc
     1141 atcgagcgct tcctcaagat cggccgggct ggccagcggc acttcggcga tcgcttcgcc
     1201 gcgcgccgga tcgactaccg cgaaggtatc gcgcccttcg cccagccgcc agctgccggc
     1261 gatgaacatg gccagatcgg catcgtaact gtgagtcata tagtttcccg ctcttgtaac
     1321 gcagcgtctc tagcgagcgg gcggctattt ggcgagaggg ggcgtcaggc ctcgtggagc
     1381 gcgttgacca gtgttgtgcg cagcgcaatc agccgtgcac gataggcaaa ggcattgggc
     1441 aacggggtcg acggctgcac agccgccggg gccggcgccg atggtgctgc caccgccgcc
     1501 gcagccttgg ccgcgccgcc ctgcagggcc ttcttggcac ccttgagggt atagccttcg
     1561 cgatggacga gccgatcgat cgtttcgacc agcacgacat cctctggccg gtagtaacgc
     1621 ctgccgccgc tacgcttgag cggcttgagc atcgggaatt gttcttccca atagcgcagg
     1681 acgtgctggc ggatacccag cgccttggcg acttcgccga tcgttcgcag agcctccgga
     1741 tccttgccat catcgaaggc gccggcgccc tcgatccggt caagaagatc cccgctgctc
     1801 atccgcgaac gatccgctct ttcagcatct ggctggcgcg gaaagtgagc acccggcgcg
     1861 gggtgatcgg cacttcgatg ccggtcttgg gattgcggcc cacgcgctgg cccttgtcac
     1921 gcagcagaaa ggttccgaaa ccggaaatct tcacgttttc gccgctctcc agcgcgttgc
     1981 tcatgtgatc gaggatcgcc tcgaccatgg tgagggattc cgagcgggac aggcccagct
     2041 tgcggttgat ggcttctgcc agatcggctc gtgtaagcgt gctcat
//
LOCUS       DASUDV010000082         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1548958,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000082 DASUDV010000000
VERSION     DASUDV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548958"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFV30_01760"
     CDS             complement(<1..700)
                     /locus_tag="VFV30_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX4846844.1"
                     /translation="MTALPNPASGNPLALARWLWSVAALVVVMIVVGGVTRLTESGLS
                     ITEWKPVTGAIPPLSAEQWDAAFEAYKRIPQYTEVNGPAGMTLDEFKYIFFWEWFHRL
                     LGRVIGLAYGLPLAWFWLRRAIPAGYHARLLGLLALGGLQGFVGWWMVKSGLTQDVKV
                     SHLRLATHLSLALTTLALLAWTALDLRALAQGKAAARLSRFGLLTLAVLAVQLFYGAL
                     VAGLRAGPVAGAGWF"
     gene            823..1194
                     /locus_tag="VFV30_01765"
     CDS             823..1194
                     /locus_tag="VFV30_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEX4846845.1"
                     /translation="MRDAMILIRNRLDRMGVILSGLCALHCIAGLLLVAGLGLGGELL
                     FAPEIHRIGLALAIVVGAITLVLGVVRHGDPRPLQLGAAGLGLMIVALVMGHNSTEAV
                     LTIAGVALLGWAHLRNLRLSN"
     gene            1212..2204
                     /gene="thiS"
                     /locus_tag="VFV30_01770"
     CDS             1212..2204
                     /gene="thiS"
                     /locus_tag="VFV30_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX4846846.1"
                     /translation="MTAQLSITLNGEPRRAAPGSIADLVRSLELDPAKVAVERNGEIV
                     PRSTLDAVALGEGDVLEIVHFVGGGQGASAGDDSWTVAGRTFRSRLIVGTGKYKDFEQ
                     NAAAVEASGAEIVTVAVRRVNVSDPKAPMLTDYIDPKKITYLPNTAGCFTAEDAIRTL
                     RLAREAGGWDLVKLEVLGEARTLYPNMVETIRACEVLAKEGFLPMVYCTDDPIAAKQL
                     EEAGAVAVMPLGAPIGSGLGIQNRVTIRLIVEGASVPVLVDAGVGTASDAAVAMELGC
                     DGVLMNTAIAEAKDPLRMARAMKLAVEAGRHAYLSGRMATRKYADPSSPLAGLI"
     gene            2274..2801
                     /locus_tag="VFV30_01775"
     CDS             2274..2801
                     /locus_tag="VFV30_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846847.1"
                     /translation="MANAGTASLTIAELREFASFTPCEQRYIKRSLDVGLGRQDAFKL
                     WARDAAETASIRSQYVVYQDLKSLRGAVPAESGFDALEEFIGKLVRIAAFDLAQERLT
                     CFSAFRFLYERLLGPDVRPFLPSAFCAAAALPQIRPERRKHLLQSISEAAATAPGWSA
                     RAPSFYPEWVEKEAA"
BASE COUNT          497 a         1013 c         1013 g          466 t
ORIGIN      
        1 agaaccagcc tgcccccgca accggcccgg cccgcagccc ggcaaccagc gcgccataga
       61 acagctgcac cgccagcacg gccagggtca gcagcccgaa gcgcgaaagc cgcgcggcgg
      121 ccttgccttg cgccagggcg cgcaggtcca gcgcggtcca ggccagcaac gccagcgtcg
      181 tcagcgccag gctgaggtga gtcgccagcc gcaggtggct gaccttgacg tcctgggtca
      241 ggccggactt gaccatccac cagccgacaa agccctgcag cccgcccagc gccagcaggc
      301 cgagcagccg ggcatggtag ccggccggaa ttgcccgccg cagccagaac caggccagcg
      361 gcagaccata ggccaggccg atgacccgcc caagcaggcg gtggaaccat tcccagaaga
      421 agatgtactt gaactcgtcc agcgtcatcc cggccgggcc gttgacctcg gtgtactggg
      481 ggatccgctt gtaggcctcg aaggccgcgt cccactgttc ggcgctgagc ggcgggatcg
      541 cgccggtcac cggtttccat tcggtgatcg acagcccgga ttcggtcagg cgggtcaccc
      601 cgccgacaac gatcatcaca accaccagcg cggcaacgga ccagagccag cgggcgaggg
      661 ccagcggatt gccgctggcg ggattcggaa gcgcggtcat ggccgcgccg tgccgcgcgc
      721 ggcggcaaag cgcaatatcc tttaagggaa tgtcgccggg caggggcctg tcgccgcgcg
      781 gggcttgatt gatgttacat agtcacataa aggggcccgg caatgcgcga tgccatgatc
      841 ctgattcgca accggttgga ccggatgggc gtgatcctgt ccgggctctg cgcgctgcac
      901 tgcattgccg gtctgctgct ggttgcgggc ctcggcctgg gcggcgaact gctgttcgcg
      961 ccggagattc accgcatcgg cctggccctg gcgatcgtgg tcggcgcgat cacgctggtc
     1021 ctcggcgtgg tgcgccacgg cgatccccgc ccgctgcaac tgggcgcggc cggcctgggg
     1081 ctgatgatcg tcgcgctggt gatgggccac aactcgaccg aagccgtgct gaccatcgcc
     1141 ggggtggcgc tgctcggctg ggcgcacctt cgcaacttgc ggctcagcaa ctgaatgcta
     1201 ttggccgggg catgactgcc cagctttcca tcacgctcaa cggggaaccg cgccgcgccg
     1261 cgcccggttc gatcgccgat ctcgtccgca gcctcgaact cgatccggcc aaggtcgcgg
     1321 ttgaacgcaa cggcgagatt gtcccgcgct cgaccctgga cgcggtggcc cttggcgagg
     1381 gcgacgtgct ggagatcgtc cacttcgtcg gcggcgggca gggcgcttcg gccggggacg
     1441 acagctggac cgtggccggc cggaccttcc gctcgcggct gatcgtcggc accggcaagt
     1501 acaaggattt cgaacagaac gccgccgcgg ttgaagcctc cggcgcggaa atcgtcacgg
     1561 tcgcggtgcg ccgggtcaac gtctcggacc ccaaggcgcc gatgctgacc gactatatcg
     1621 atccgaagaa gattacctac ctgcccaaca ccgccggctg cttcaccgcc gaagacgcga
     1681 tccgcaccct gcggctggcg cgcgaggcgg gcggctggga tctggtcaag ctggaagtgc
     1741 tgggcgaggc gcgcacgctt tatcccaaca tggtcgaaac gatccgcgcc tgcgaagtcc
     1801 tggccaagga gggcttcctg ccgatggtct attgcaccga cgatccgatc gcggccaagc
     1861 agctggaaga ggccggggcc gtggcggtca tgccgctcgg cgcgccgatc ggctcgggcc
     1921 ttggcatcca gaaccgggtg acgatccgcc tgatcgtcga gggcgcttcg gtgccggtgc
     1981 tggtcgacgc cggggttggc accgccagtg atgcggccgt ggcgatggaa ctgggctgcg
     2041 acggggtgct gatgaacacc gcgattgccg aggcgaagga tccgctgcgg atggcccggg
     2101 cgatgaagct ggcggtcgag gccgggcggc acgcctacct ctctggccgg atggcgaccc
     2161 gcaagtacgc ggatccctcc agcccgctgg caggccttat ttaggcacca tggttaacgg
     2221 cttcataacc atattctggt gatctctccc ccgaaccaac tgggggcaag accatggcca
     2281 atgccggaac cgcatcgctg accatcgccg aactgcgcga gtttgccagc ttcacgccct
     2341 gcgagcagcg ctacatcaag cgcagcctcg atgtcgggct gggccggcag gacgccttca
     2401 agctgtgggc ccgcgacgcg gccgagaccg cctcgatccg cagccagtac gtggtctacc
     2461 aggacctcaa gagcctgcgc ggcgcggtgc cggcggaatc gggctttgac gcgctggagg
     2521 aattcatcgg caaactggtc cggatcgccg ccttcgacct ggcccaggaa cgcctgacct
     2581 gcttctcggc cttccggttt ctgtacgagc gcctgctcgg tccggatgtc cgtccgttcc
     2641 tgcccagcgc cttctgcgcg gcggcggcgc tgccgcaaat ccgccccgaa cggcgcaagc
     2701 atctgctcca gtcgatcagc gaagcggcgg ccacggcccc cggctggtcg gcgcgcgcac
     2761 ccagcttcta tcccgaatgg gtggaaaaag aggcggcctg aatcgctttt tctgcgcgcg
     2821 gcgcaatttt cggaatcgac aatcgccccg gaagcgggca aaccccgcgc tgctcgccca
     2881 tggcgggcaa gggttaacac aaggggattc ccaatgaaga agattgctct gttcgccgcc
     2941 atcgcggctg gctcgctcgc cgtcgccgct tgcggtgaaa agaaggaag
//
LOCUS       DASUDV010000083         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_400371,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000083 DASUDV010000000
VERSION     DASUDV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400371"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFV30_01780"
     CDS             <1..560
                     /locus_tag="VFV30_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4846848.1"
                     /translation="TDTTAQVGVRATLPIFQGGLPAAQKRQAQAREGALLEQEIATER
                     AVIANVRAAYAQYQAANEIIEANKAAVAAAELSLEGVRAENTVGNRTILDILNAEQEL
                     LSAQVQLATAERNGYVAGFNLLAAMGKAEARDLALDGGALYDPQANYDRVKGSMFDWA
                     TDPAPKAKATRTVDTKPQDGTVTAK"
     gene            597..1040
                     /locus_tag="VFV30_01785"
     CDS             597..1040
                     /locus_tag="VFV30_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX4846849.1"
                     /translation="MRQASEPSVEEILDSIKKVIARDNRADAAVVRKEREERGVKAAE
                     AEAEDDVFELDAAAELVDDEAAEDAPLLPESTASSMRESIAALAMLAEPGVAPQIVRS
                     GETSLEGLTRELLRPALAEWLDKNLPPLVERMVAAEIARIVGKKG"
     gene            1077..1421
                     /locus_tag="VFV30_01790"
     CDS             1077..1421
                     /locus_tag="VFV30_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX4846850.1"
                     /translation="MAERTLARLGGFDTRWQIFLCADPEEPKCCDRAKGHEAWLFLKK
                     RLGELGLGGHTGVLRNKVGCLRVCALGPVAVVWPGPTWYHSCTPEVLERIIQEHLIGG
                     VPVAEYRLNPPA"
     gene            complement(1422..1694)
                     /locus_tag="VFV30_01795"
     CDS             complement(1422..1694)
                     /locus_tag="VFV30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846851.1"
                     /translation="MTRSPLENPEIAAIAWSRYKRLLKRWSLVTLAVCLVLIAYLAWE
                     FGLPSIHLYIASVLGIAGVIMLTVALMALLFLSSGTGHDESIDERE"
     gene            1804..2232
                     /locus_tag="VFV30_01800"
     CDS             1804..2232
                     /locus_tag="VFV30_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4846852.1"
                     /translation="MTIARLIEGRSGPIISIDASASVREAVALLAEKRIGALPVMQGG
                     AIAGIFSERDVVYGLAQNGPAMLDGPVGAVMTAPVISVDPQSDVLDALGLMTRRRIRH
                     LPVVEGGAMVGFISIGDLVKYRIERIESEAQAMRSYIQSA"
     gene            2272..>2539
                     /locus_tag="VFV30_01805"
     CDS             2272..>2539
                     /locus_tag="VFV30_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX4846853.1"
                     /translation="MDAPTMTLSPAAAKRVAFIAEKQGKPAALRLAVEGGGCSGFQYR
                     FGLAESIEEDDLVVETDGVKLVVDPISLDLVAGSVVDFVDSLGGS"
BASE COUNT          456 a          821 c          860 g          402 t
ORIGIN      
        1 agaccgatac gaccgcgcag gtcggcgtgc gcgccaccct gccgatcttc cagggcggct
       61 tgccggccgc gcagaagcgc caggcccagg cccgcgaggg cgcactgctg gagcaggaga
      121 ttgccaccga gcgcgcagtc atcgccaatg ttcgcgccgc ctatgcccag taccaggccg
      181 cgaacgagat catcgaggcc aacaaggccg ccgtggccgc cgccgagctg agccttgaag
      241 gcgtgcgggc ggaaaacacc gtcggcaacc ggacgattct cgacatcctc aatgccgagc
      301 aggaactact cagcgcccag gtccagctcg ccacggcaga gcgcaatggt tacgttgcgg
      361 ggttcaacct gctggcggcc atgggcaagg ccgaagcccg cgatctggcg ctcgatggcg
      421 gggcgctgta cgatccccag gccaattatg accgggtcaa gggcagcatg ttcgactggg
      481 cgaccgaccc ggccccgaag gccaaggcca cccggaccgt tgacaccaag ccccaggacg
      541 ggacagtaac cgccaagtga ttctgtcccc ctgcgggaca attgggggtt gggaccatgc
      601 gccaggcaag tgaaccgtcg gtcgaagaga tcctcgattc gatcaagaag gtgatcgcgc
      661 gcgacaatcg cgccgatgct gccgtcgtcc gcaaggaacg cgaagagcgc ggggtcaagg
      721 ctgccgaggc tgaagccgag gacgatgtct tcgaactcga tgccgctgcc gaactggtcg
      781 acgacgaggc ggccgaggat gccccgctgc tgcccgaaag caccgccagc tcgatgcgcg
      841 aatccatcgc cgcgctcgcc atgctggccg aacccggcgt ggcgccgcag attgtccgct
      901 cgggcgaaac ctcgctcgaa gggctgaccc gcgaactgtt gcgcccggcg ctggccgaat
      961 ggctcgacaa gaacctgccg ccgctggttg agcggatggt tgcggccgag atcgcccgga
     1021 tcgtcggcaa gaagggctga gccctgcccg atttaccgga tcctgccgaa cgcgaactgg
     1081 ccgaacgcac gctcgcccgg ctgggcgggt ttgacacgcg ctggcagatc ttcctctgcg
     1141 ccgatcccga agaacccaag tgctgcgatc gcgccaaggg gcacgaggcc tggttgttcc
     1201 tcaagaagcg gctcggcgaa ctggggctgg gtggccatac cggggtgctg cgcaacaagg
     1261 tcggctgcct cagggtctgt gcgctcgggc ccgtggcggt cgtctggccc gggccgacct
     1321 ggtatcactc gtgcacgccg gaggtgcttg agcgaattat ccaggagcac ctgattggcg
     1381 gcgtgccggt tgcggaatac cgcctcaacc cgccggcctg actactcgcg ctcgtcaatc
     1441 gactcgtcgt ggccggtgcc gctggacagg aacagcagcg ccatcagcgc cacggtcagc
     1501 atgatcacac ccgcgatccc cagcacgctg gcgatgtaga ggtgaatcga aggcaggccg
     1561 aattcccagg cgagataggc aatcagcacc aggcagacgg ccagcgtcac caggctccag
     1621 cgcttgagca gccgcttgta gcgcgaccag gcaatcgcgg cgatttcagg attttcgagc
     1681 ggggaacggg tcatcgccgc cgcctttgcg cctccaccac cgcccgcgca agtggacgtt
     1741 agtcccggac tcgccaaccc gcgcgcttcg tgtcatcttc ccgcctatag aggggagtat
     1801 gccatgacta ttgcccgcct gatcgagggg cgcagcggcc ccattatctc catagatgcc
     1861 agcgccagcg tgcgcgaagc cgtggccctg ctggctgaaa agcggatcgg tgcgctgccg
     1921 gtgatgcagg gcggcgcaat cgccgggatc ttttccgagc gcgacgtggt ctatggcctg
     1981 gcacagaatg gccccgccat gctcgatggg ccggtaggtg cggtgatgac cgcgccggtg
     2041 atctcggtcg atccgcagtc cgacgtgctc gatgcgctgg gcctgatgac ccgacgccgt
     2101 attcgccacc tgccggtggt agaaggcggg gcgatggtcg gcttcatttc gatcggcgac
     2161 ctggtgaaat accggattga gcggatcgaa tccgaggccc aggcgatgcg cagctatatc
     2221 cagagcgcct gaatccttgc gggtcgggcc ggcgcggcct acatcgggga catggacgcc
     2281 ccgacgatga ccctcagccc cgcagcggcc aagcgcgtgg cgtttattgc cgaaaagcag
     2341 ggcaagccgg ctgcgctgcg cctcgccgtg gaaggtggcg gctgctcggg ctttcaatac
     2401 cggttcggtc tggccgagag catcgaagaa gatgacctgg tggtcgaaac cgatggcgtg
     2461 aagctggtgg ttgacccgat cagcctcgat ctggtcgcgg gaagcgtggt cgatttcgtc
     2521 gattcgctcg gcggctccg
//
LOCUS       DASUDV010000084         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1284059,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000084 DASUDV010000000
VERSION     DASUDV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284059"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFV30_01810"
     CDS             <1..705
                     /locus_tag="VFV30_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4846854.1"
                     /translation="SVAVIGAGDFIGAAIVRKFAGEGFAVHAGRRNGDKLAPLLGGPV
                     TGRSLDAREPDDVAAFLAEAEAIAPLALVVFNVGANVQFPLLETTDRVFRKVWEMACF
                     AGFVSVREAARIMAPRGAGKIFVTGATAGLRGNPGYAAFAAAKAGLRAMAQSAAREWW
                     PKGLHVAHLVIDAGVDTEWVRQRVTERIGAEALAAQPDLLMPPEAVAEAYWALYNQPK
                     AAWTFEQEIRPYGETW"
     gene            705..1304
                     /locus_tag="VFV30_01815"
     CDS             705..1304
                     /locus_tag="VFV30_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX4846855.1"
                     /translation="MTEIEFLYDFGGPNSWFVHRAIPAIEAQGKVRFRYSPVLLGGLF
                     KATGNKPPLMTYGHVAAKVAYDRLEMDRFMARHAITGFRFNPHFPVNTLLAMRGAVAA
                     ERLGCGPAYREAMYTALWQDGLKLDDPEVWAGAVTAAGLDAAALGALVQDDAVKAELT
                     ANTERAVARGAFGVPTFFLGDEMWFGKDRLRDVVEAAGG"
     gene            1385..2173
                     /locus_tag="VFV30_01820"
     CDS             1385..2173
                     /locus_tag="VFV30_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4846856.1"
                     /translation="MSDYPLPYNSTDLSGRVALVTGASSGLGARFAEVLASQGAAVVL
                     AARRLDRLESLAEEIRARGGKALAVQMDATDAESLIAAVAKGEEAFGTIDILVNNAGM
                     PDAQRAHKMSLELIDQVLGVNLRGPWILACEVARRLIAAGKPGRIVNISSVAHFRYDG
                     GGAALYAVTKTAIARMTEALSVEWAHYNINVNAIAPGMFVSEMTDGMFARMGGDPAPH
                     LPRKRVCVPEQLDSTLLYLVAPASECVTGATIRVDDGQSARTRI"
     gene            2186..>2724
                     /locus_tag="VFV30_01825"
     CDS             2186..>2724
                     /locus_tag="VFV30_01825"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4846857.1"
                     /translation="MLVTVERDGPVAVVTLNRPEAMNALSRGLRAQLAQVMVELDAEE
                     RIRAVVLTGAGTRAFTAGLDLKELGSGAASLGAATDQEATSNPVKAIETCRKPVIGAI
                     NGVAITGGFELALACDILIASENARFADTHARVGIMPGWGLSQKLSRMIGISRAKELS
                     LTGNFIDAQTACAWGLVNRV"
BASE COUNT          435 a          884 c          962 g          443 t
ORIGIN      
        1 tcggtcgcgg tgatcggcgc gggcgatttc atcggcgcgg cgatcgtccg caaattcgcg
       61 ggtgagggct ttgccgtcca cgccggccgc cgcaacggag acaagctcgc cccgctgctg
      121 ggcgggccgg tcaccggccg cagcctcgac gcgcgcgagc cggacgatgt cgcggccttc
      181 ctggccgagg ccgaggccat tgccccgctc gcgctggtgg tgttcaacgt cggcgccaac
      241 gtgcagttcc cgctgctgga aacgaccgac cgggtgttcc gcaaggtctg ggaaatggcc
      301 tgcttcgccg gcttcgtctc cgtgcgcgag gcggcgcgga tcatggcgcc gcgcggggcc
      361 ggcaagatct ttgtcaccgg ggcgaccgcc gggctgcgcg gcaatccggg ctatgccgcc
      421 tttgccgcgg ccaaggcggg gctgcgcgcc atggcccagt cggccgcgcg cgagtggtgg
      481 ccgaaggggc ttcacgtcgc ccacctggtg atcgatgccg gggtcgacac cgaatgggtc
      541 cgccagcggg tgaccgagcg gatcggggcc gaggcgctgg ccgcccagcc cgacctcttg
      601 atgccgcccg aagccgtggc cgaggcctat tgggcgctat acaaccagcc gaaggcggcc
      661 tggacgtttg agcaggaaat ccggccctat ggggagacct ggtgatgacg gagatcgagt
      721 tcctctacga tttcggcggg cccaattcgt ggttcgtcca ccgcgcgatc ccggcgatcg
      781 aggcccaggg caaggtccgc ttccgctatt ccccggtgct gctgggcggg ctgttcaagg
      841 ccaccggcaa caagccgccg ctgatgacct atggccacgt tgcggccaag gtggcctatg
      901 accggctgga aatggaccgg ttcatggccc gccacgccat caccggcttc cggttcaacc
      961 cccacttccc ggtcaacacc ctgctcgcca tgcgcggcgc ggtcgcggcc gagcggctgg
     1021 gctgtggtcc ggcctatcgc gaggcgatgt acaccgcgct gtggcaggac gggctgaagc
     1081 tggacgatcc cgaggtctgg gccggggcgg tgaccgccgc aggactggat gccgcggcgc
     1141 tgggtgcgct ggtccaggac gatgcggtga aggccgaact gactgccaac accgaacgcg
     1201 ccgtcgcgcg cggcgccttt ggcgtgccga ccttcttcct gggtgacgag atgtggttcg
     1261 gcaaggatcg gttgcgcgac gtggtggagg cggcgggcgg ctgacgcttg ccaagccgcc
     1321 cggctccatg catcgtgcga tttaatcgcg cgattgaaat ggcgcgccga gcggagatcc
     1381 cgagatgagc gactatcccc tgccctacaa ttcgaccgac ctttccggcc gggttgcgct
     1441 ggttaccggc gcctcctcgg gcctgggcgc acgctttgcc gaagtgcttg ccagccaggg
     1501 cgcagcagtc gtgctggccg cgcggcggct tgaccggctc gagtcgctgg ccgaggaaat
     1561 ccgcgcccgc ggcggcaagg cgcttgcggt tcagatggac gcaaccgatg ccgaaagcct
     1621 gatcgcggcc gtagccaagg gcgaagaggc cttcggtaca atcgacatcc tggtcaacaa
     1681 cgccgggatg cccgatgccc agcgtgccca caagatgagc ctggaactga tcgaccaggt
     1741 gctgggggta aacctgcggg ggccctggat cctggcctgt gaagtcgccc gccggctgat
     1801 cgcggcgggc aagcccgggc ggatcgtcaa tatcagttcg gtcgcgcact ttcggtatga
     1861 tggcggcggc gcggcgctct atgccgtaac caagacggcg attgcccgga tgaccgaggc
     1921 actaagcgtc gaatgggcgc actacaacat caacgtcaac gcgatcgcgc cggggatgtt
     1981 cgtttcggaa atgaccgacg gaatgttcgc gcgaatgggg ggcgatcccg caccgcacct
     2041 gccgcgcaag cgcgtctgcg ttcccgagca actcgattcg accctgctgt acctggttgc
     2101 tccagccagc gaatgcgtca ctggcgcgac gatccgcgtt gacgacgggc aaagcgcgcg
     2161 cacgcggatc tagaaggaaa cgatcatgct ggtcacagtc gagcgcgatg gcccggtagc
     2221 agtggttacg ctcaatcgcc ccgaggcgat gaatgcactg tcgcgcgggc tgcgcgcaca
     2281 gctcgcacag gtcatggtcg agctggacgc cgaagagcgg atccgcgccg tggtgctgac
     2341 cggagcggga acccgcgcct ttaccgccgg gcttgatctc aaggagctgg gctcgggcgc
     2401 ggccagcctg ggcgcggcga ccgatcagga ggcaaccagc aatccggtca aggcgatcga
     2461 aacttgccgc aagccggtga tcggcgcgat caacggcgtt gcgattactg gcgggttcga
     2521 gctggcgctg gcctgcgaca tcctgatcgc cagcgagaat gcacgctttg ccgataccca
     2581 tgcccgggtc gggatcatgc cgggctgggg cctttcgcag aagctcagcc ggatgatcgg
     2641 tatctcgcgg gccaaggaac tgtcgctcac cggcaatttc atcgatgccc agaccgcctg
     2701 tgcctggggc ctggtcaacc gcgt
//
LOCUS       DASUDV010000085         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1372280,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000085 DASUDV010000000
VERSION     DASUDV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372280"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFV30_01830"
     CDS             complement(<1..653)
                     /locus_tag="VFV30_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEX4846858.1"
                     /translation="MRSMAGDGALRRVLPWFLGASALGGVALTWALLAPAPQRVLYSE
                     LSDAERAGVVASLDKAGIAYAIDNQTGTLSVGEDDLYRARALVAQDGGLAVPDAANSL
                     DSLPLGASRTLEGERLRAVREHELTLTIQEIDGIESVRVHLAEAAKSVFVRDNAPPSA
                     SVMVRMARGRQLGDSQVAAVVNLVAGSVPGMSPDAVRVVDQHGRLLSAKGSAENDRL"
     gene            complement(726..1094)
                     /gene="fliE"
                     /locus_tag="VFV30_01835"
     CDS             complement(726..1094)
                     /gene="fliE"
                     /locus_tag="VFV30_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX4846859.1"
                     /translation="MNGPGAIGSAGSIQQIMAMRQQILDRSQLLQQVHTAKAGDNSPV
                     QPQGPAGDFTNALQSALDSVTATQRNAESLTTAYERGEVTDIAKVMLARQEAGIAFEA
                     TLQVRNRLLSAYQDIMRMGV"
     gene            complement(1091..2341)
                     /locus_tag="VFV30_01840"
     CDS             complement(1091..2341)
                     /locus_tag="VFV30_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4846860.1"
                     /translation="MTGLSLGENAAKQHAGLVDRAAVIMATLAADGPAHIRTRGDALP
                     PGPVPCVSLERAEASFVRRESGRHHLTLGYRDGSDEAPLAALLAALAQPGMPVAADPE
                     SLSVLALAERLAASEIPVLVGGPTGTGKEVLSRFIHARSPRAAGPFVAVNCAAMPESM
                     LEALLFGHRKGAFTGASEANEGFFRAADGGTLLLDEIAEMPLPLQAKLLRALQEGEVV
                     PIGSTQPIKVDVRVIACANRDLPVEVAEGRFRADLFYRLNVFPLALRPLRERPQDVAP
                     LAFAMVLRHAAPQRPIPWIGDDALALLKAHAWPGNVRELENVMRRAMLLADRDPQIDA
                     RHIRFDTGVRLASDDGVPSAPEDSRLSSIVQHSEARAIMATLDACGGNRLAAARELGI
                     SERTLRYRLAAFREAGLAVAGGRR"
BASE COUNT          392 a          822 c          815 g          378 t
ORIGIN      
        1 tccagccggt cgttttcggc gctgcccttg gccgaaagca gccgaccgtg ctgatcgaca
       61 acccgcaccg catccgggct catccccggg accgatccgg caaccaggtt gaccactgcg
      121 gcaacctggc tgtcgccgag ctgccggcca cgggccatgc ggaccataac cgaggcagaa
      181 ggcggggcat tgtcgcgcac gaagaccgat ttggccgcct cggccagatg cacgcgcaca
      241 ctctcgatcc cgtcgatttc ctggatcgtc agggtcagct cgtgttcgcg cactgcgcgc
      301 agccgctcac cctccagcgt ccggctggca cccagtggca gggaatcgag cgagtttgca
      361 gcatcgggaa ccgcaaggcc gccatcctgg gcaaccagcg cccgggcgcg atagagatcg
      421 tcttcgccca cgctcagggt gccggtctgg ttgtcgatcg cataggcgat gcccgccttg
      481 tcgagcgagg caaccacgcc ggcgcgttcg gcatcgctga gttcggaata gagcacccgc
      541 tgcggggcag gggcaagcag cgcccaggtc agcgcgacgc cgcccagcgc gctcgcgcca
      601 agaaaccagg gcaggacccg gcgcaaggcg ccatccccgg ccatggatcg catccgttcg
      661 agcggattgg ccggcgcccc agccagcgcg gcgggatcgt tggtggtgat cagctcagcc
      721 attcatcata ctcccatccg catgatgtcc tggtaggcag agagcagccg gttccgaacc
      781 tgcagcgtcg cttcgaaggc aatcccggct tcctggcgcg ccagcatgac cttggcgatg
      841 tcggtcactt cgccgcgttc gtaggcagtg gtcaggcttt cggcgttgcg ctgggtcgca
      901 gtgacgctgt cgagcgcgga ttgcagcgca ttggtgaaat cgcccgccgg gccctggggc
      961 tggaccggag aattgtcccc ggccttggcg gtatgaacct gctgaagcag ctgcgagcga
     1021 tcgaggatct gctggcgcat cgccatgatc tgctggatcg atccggcact gccgatcgcg
     1081 cccggcccgt tcatcgccgg cctccggcca cggccagccc ggcctcgcgg aaggcggcaa
     1141 ggcgatagcg cagcgtgcgc tcggaaatgc ccagttcgcg cgcggcggcc aggcggttgc
     1201 cgccgcaggc atcaagcgtt gccatgatcg cgcgggcttc ggaatgctgg acgatgctcg
     1261 acaatctgct gtcctccggc gcgctcggca ccccatcgtc gctcgccagc ctgacgccag
     1321 tatcgaagcg gatatgccgg gcatcgatct gcgggtcccg gtcggcgagc agcattgcgc
     1381 ggcgcatcac gttctccagc tcgcgtacgt tgccgggcca ggcatgggcc ttgagcagcg
     1441 ccagtgcgtc gtcgccgatc caggggatgg gccgctgcgg cgcggcgtgg cgcagcacca
     1501 tggcaaaggc cagcggggcc acatcctggg gtcgttcgcg caagggacgc agcgccagcg
     1561 ggaacacgtt gagccgatag aacaggtcgg cccggaacct cccttcggca acttcgaccg
     1621 gcaggtcacg gttggcgcag gcgatcaccc ggacatcgac cttgatcggc tgggtcgaac
     1681 cgatcggcac cacttcgcct tcctgaaggg cgcgcagcag tttggcctgg agcggcagcg
     1741 gcatctcggc gatctcgtcc agcagcaggg ttccgccatc ggcggcgcgg aaaaaccctt
     1801 cattggcctc gctcgccccg gtgaaggcgc ccttgcgatg gccgaacagc aaggcttcga
     1861 gcatgctctc gggcatcgcc gcgcagttga ccgcgacgaa ggggccagcc gcgcgcgggc
     1921 tgcgggcatg gatgaagcgg ctcagcactt ccttgccggt gccggtcggg ccgcccacca
     1981 ggaccggaat ttcgcttgcc gccagccgtt cggccagggc cagcaccgag aggctttcgg
     2041 gatcggcggc aaccggcatg cccggctggg ccagcgcagc gagcagtgcg gccagcggcg
     2101 cctcgtcact accatcgcga taacccagcg tcagatgatg gcgcccgctc tcacggcgaa
     2161 caaagctggc ttcggcccgt tcaagcgaga cgcaagggac ggggccgggg ggcagagcat
     2221 cgcccctggt gcggatgtgc gcgggaccat cggcggctag cgtcgccatg atcaccgccg
     2281 cacggtcgac caagccggca tgctgctttg ccgcgttttc gccaaggctc aggcctgtca
     2341 tcgagatacc ccccggttaa ccatcgtcac gaggggtctt gtgcgcgctg aacgtcaaac
     2401 gctccgg
//
LOCUS       DASUDV010000086         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_422604,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000086 DASUDV010000000
VERSION     DASUDV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422604"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1021
                     /locus_tag="VFV30_01845"
     CDS             47..1021
                     /locus_tag="VFV30_01845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX4846861.1"
                     /translation="MQRGIDFLGSEHAILCGAMSWVSERNLVAAMSNAGGFGVIACGA
                     MTPDLLDAEIAATKALTGRPFGVNLITMHPLLFDLIAVCAKHKVSHVVLAGGIPPKGS
                     VEAIKAFGAKVLVFAPTLALARRLLRSGADALVIEGSEAGGHIGPVATSVLAQEFLPE
                     LAEEFVVFVAGGIGRGDMIAAYLEMGASGVQLGTRFACASESIAHPDFKAAFFRANAR
                     DAVASVQVDPRLPVIPVRALKNKGTEEFTAKQIEVAKLLDAGEVDMEQAQLEIERFWA
                     GALRRAVIDGDVETGSVMAGQSVGMVTKEEPVADIIAQLMRESEAALA"
     gene            1071..1538
                     /locus_tag="VFV30_01850"
     CDS             1071..1538
                     /locus_tag="VFV30_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4846862.1"
                     /translation="MDTPEDLIPLDPAYAKMLRVRACLHGAILLALALVGEVFAPTPT
                     GLIAVPALALAAWLIGVVPQRRYARWGYAFGADRLRVVSGYLFYNDTVVPLGRIQHID
                     LDQGPLMRRWDLAELTVHTAGSHGASVSLPGLRRADAEAMREAIREHIRKALD"
     gene            1541..3034
                     /locus_tag="VFV30_01855"
     CDS             1541..3034
                     /locus_tag="VFV30_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4846863.1"
                     /translation="MDEKDAAPPAAERRRLSASGLLVNTVKELPGLFVPILAGLYGTR
                     NSDIGALPVIAGALVLGLGWHALVWLRHFYTVSDDDIRIEKGLINRTTRSIPFERIVD
                     VTIERPALARVMGLAVVRFDTGGGESDEAALRYVTQAEAERLRQTVRSHRTGAPSAEH
                     DLAAVEPPSTVLFAMDPRRIVTLGLYSFSLVIFGVLIGLSAKFDFLMPDWPLWLELLE
                     ARGEEASHLSLAQQGLGLIAALAALIAIGIGTGIVRVALREWGFVLERTPKGLRRRRG
                     LLTHTDMTVSLPRVQLAEIGTGAIRARRGWHWLTLASLAGAGGEDGGGVVAPLARLEE
                     IWPILREAGLTPPADGTDFTRPVFAPVRDWIVLRSLALGALGLAAHGLGLPYAWLVAV
                     PMAWLALRDWLRWRQKSGAVDADQLYLKAGWWTRLLVIARQINVQSVSVSSSPLERRH
                     GLARVDFGIPNGAMHLGPMPLAEALAIRDSVLAIAAPVDFSRLNRPH"
     gene            complement(3174..>3435)
                     /locus_tag="VFV30_01860"
     CDS             complement(3174..>3435)
                     /locus_tag="VFV30_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX4846864.1"
                     /translation="GFVANPAWGGQGWIDYTAPVAKAGEFDLYGQVMTQIWAVGTTVL
                     WTGVVSTVLFLGLKYTIGLRPTVEAEREGLDINEHGERAYNY"
BASE COUNT          496 a         1188 c         1199 g          552 t
ORIGIN      
        1 tacgacatcc gaggcctttc ccgtgtatga aaaaacccac gcgctgatgc agcgcggtat
       61 cgacttcctg gggagcgagc acgccatcct gtgcggagcc atgagctggg tgtccgaacg
      121 caacctggtg gcggccatgt ccaacgccgg cggctttggc gtgatcgcct gcggggcgat
      181 gaccccggac ctgctggatg ccgagatcgc cgcgaccaag gcgctgaccg ggagaccctt
      241 cggcgtcaac ctgatcacca tgcacccgct gctgttcgat ctgatcgcgg tctgcgccaa
      301 gcacaaggtc agccacgtgg tgctggccgg cgggatcccg cccaagggca gtgtcgaagc
      361 gatcaaggcc tttggcgcca aggtgctggt ctttgccccc accctggccc tggccaggcg
      421 cctgctgcgc agcggcgccg atgccctggt gatcgagggc agcgaagccg gcggccatat
      481 cggcccggtg gcaaccagcg tgctggcgca ggaattcctg cccgaactgg ccgaggaatt
      541 cgtggtcttc gtggccggcg ggatcggccg gggcgacatg atcgccgcct acctggaaat
      601 gggcgcctcg ggcgtgcagc tcggcacccg ctttgcctgt gccagcgaaa gcatcgccca
      661 ccccgatttc aaggcggcct tcttccgcgc caacgcccgc gacgcggtcg cctcggtcca
      721 ggtcgatccg cgcctgccgg tgatcccggt ccgcgcgctg aagaacaagg gcaccgagga
      781 attcaccgcc aagcagatcg aagtcgccaa gctgctcgac gcgggcgaag tcgacatgga
      841 acaggcccag cttgagatcg aacggttctg ggccggcgcg ctgcgccgcg cggtgatcga
      901 cggcgatgtg gagaccggat cggtgatggc cggccagtcg gtcggcatgg tcaccaagga
      961 agagccggtg gccgatatca tcgcccagct gatgcgcgag agcgaagcgg cgctggcctg
     1021 acgccgcgcc cgccttgcgc ggcgcggccg gtccggacat aacccggcgc gtggacacgc
     1081 ccgaagacct gatcccgctg gatcccgcct atgccaagat gctgcgggtg cgcgcctgtc
     1141 tgcacggggc gatcctgctt gcgctggcgc tggtgggtga ggtctttgcg cccaccccga
     1201 ccgggctgat cgcggtgccc gcgctcgcgc tggccgcctg gctgatcggc gtcgtcccgc
     1261 agcgccgcta tgcccgctgg ggctatgcct ttggcgcgga ccggctgcgg gtggtgagcg
     1321 gctatctgtt ctacaacgac acggtcgtgc cgctcggccg gatccagcac atcgatctgg
     1381 atcagggccc gctgatgcgg cgctgggatc tggcggagct gaccgtccac accgccggca
     1441 gccacggcgc ctcggtcagc ctgccggggc tgcgccgcgc cgatgccgag gccatgcgcg
     1501 aggcaatccg cgaacacatc cgcaaggcgc tggactaggc gtggacgaaa aggacgccgc
     1561 gccgcccgcc gccgagcgcc gccgcctgag tgccagcggc ctgctggtca acacggtcaa
     1621 ggaactgccc ggcctgttcg tgccgatcct ggccgggctc tacggcaccc gcaattccga
     1681 tatcggcgcc ctccccgtga ttgccggcgc gctggtgctg ggcctcggct ggcatgcgct
     1741 ggtctggctg cgccacttct acacggtcag cgacgatgac attcgcatcg agaagggttt
     1801 gatcaaccgc accacccgct cgatcccgtt cgaacggatc gtcgatgtca ccatcgaacg
     1861 gcccgcgctg gcccgggtga tgggcctcgc cgtggtgcgc tttgacaccg gcggcggcga
     1921 aagcgacgag gccgcgctgc gctatgtcac gcaggccgag gccgaacgcc tgcgccagac
     1981 ggtgcggtcg caccgcaccg gcgccccctc cgccgagcac gatctggccg ccgtagagcc
     2041 gccctccacc gtcctcttcg cgatggaccc gcgccggatc gtcacgctgg gcctctattc
     2101 gttcagcctg gtgatctttg gcgtgctgat cggcctgtcc gccaagttcg actttctgat
     2161 gccggactgg ccgctctggc tggaattgct cgaggcccgc ggcgaagagg ccagccacct
     2221 ctccctcgcg cagcagggcc tggggctgat cgcggcgctg gcggcgctga tcgcgatcgg
     2281 gatcggcacg gggatcgtcc gggtggcgct gcgcgaatgg ggtttcgtgc tggaacggac
     2341 gcccaagggc ctgcggcgcc ggcgcggcct gttgacccac accgacatga ccgtctcgtt
     2401 gccgcgggtc cagttggccg aaatcggtac cggcgcgatc cgggcgcggc gcggctggca
     2461 ctggctgacc ctggcctcgc tggccggtgc cggcggtgag gatggcggcg gggtggtcgc
     2521 gccgctggcc cggctggagg agatctggcc gatcctgcgc gaagccggac tgaccccgcc
     2581 ggcggacggc acggacttca cccgccccgt ctttgccccc gtgcgcgact ggatcgtgct
     2641 gcgcagcctg gcgctgggcg cactgggtct ggcggcccat ggtctcggcc tgccctatgc
     2701 ctggctggtt gccgtgccga tggcctggct ggcgctgcgc gattggctgc gctggcggca
     2761 gaaatcgggc gcggtcgatg ccgatcagct ttacctcaag gccgggtggt ggacgcggct
     2821 gctggtcatc gcccggcaga tcaacgtcca gtccgtctcg gtcagcagca gcccgctgga
     2881 gcggcgccac ggcctcgccc gggtggactt cggcatcccc aatggtgcga tgcacctcgg
     2941 ccccatgcca ctggccgaag ccctcgcgat ccgcgacagc gtgttggcga ttgccgcccc
     3001 ggtcgatttc tcgcgcctca accgcccgca ctgagcgggt ggccttccgc ccctggcgga
     3061 caaaaaaggg gccggagctt gcgctccggc ccgttcagtt tgcgttcgca ggaggaacgc
     3121 tgggaggcgg atccgggagg aggagaggag gaaccccgga cccgccaagc tcgtcagtaa
     3181 ttgtaagcgc gttcgccgtg ctcgttgatg tcgagccctt cgcgctcggc ctcaaccgtc
     3241 ggccgcagcc cgatggtgta cttcaggccc aggaacagca cggtggagac aacgccggtc
     3301 cacaggaccg tggtgccaac cgcccagatc tgggtcatca cctggccata gaggtcgaac
     3361 tcgccagcct tggccaccgg agcggtgtag tcgatccagc cctggccgcc ccaggcgggg
     3421 ttggcgacaa agccg
//
LOCUS       DASUDV010000087         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2123330,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000087 DASUDV010000000
VERSION     DASUDV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123330"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFV30_01865"
     CDS             <1..585
                     /locus_tag="VFV30_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846865.1"
                     /translation="IVRTPANPAEERDHALNTLLSNQLVLGQYAAFGTDVKLPLADPK
                     SAASMFTRGKVSDGYPCAPLAVTVAALARNPQDIPGRLCLGDFFRINGLDYLRGEFDP
                     APRPDELGGAAKGFAGKRNFRSEFYTSIIADPRAGANDKAYALYRAVMCYAPSAVNAC
                     GGASVPKSQRKAWFDRLKREYPQSAWAKKLRYYW"
     gene            582..1286
                     /locus_tag="VFV30_01870"
     CDS             582..1286
                     /locus_tag="VFV30_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HEX4846866.1"
                     /translation="MRRLALALALALAACAPGGSSTVRAEDHDAFFLWAGVRPQPVLK
                     EARELYLLAGEVRRDGRYLPLRAVPRAGQAELWLVVRAQSLDWAPGTLERVLADLERW
                     QAAGNRMAGLQLDFDSSTKGLGGYALFLADLRSRLDPQFKLSITGLMDWGANADPAAL
                     AGLKGVVDEIVVQSYQGRETIPGYEHYLARLARVPVPHKVALVQGGEWREPAALNEDP
                     LFRGYVVFLVNPDPGR"
     gene            complement(1291..>2269)
                     /locus_tag="VFV30_01875"
     CDS             complement(1291..>2269)
                     /locus_tag="VFV30_01875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4846867.1"
                     /translation="KLEVDADMLAIAWRYLTGSDSQVVRAIDRRLQLREPVTAEWLAE
                     ELGPQQNGDDAALLAQLMERLENSIDEFGRTSRDAQTATREYNSALEVHVSELEQVTK
                     AGDVISELATIAKVMMRRSRELEKQMLRSEAQSRTLRRRLDEARHTADHDHLTGLPNR
                     RAFETLFEEQYRQARAAMEPLCVAFCDIDHFKAINDGHGHEAGDRVLKLVAECLAHLS
                     NDRCHVARHGGEEFVLLLRGDTQAEAVAKLDLLREQLAARRLVNRATDEPFGQVTFSA
                     GVADVFACGDPRTALRAADGALYRAKNSGRNRVEPAVDADCRPPHAAAA"
BASE COUNT          316 a          771 c          805 g          377 t
ORIGIN      
        1 atcgtccgca ccccggccaa tccggcggag gagcgggacc acgcgctgaa caccttgctc
       61 tccaaccagc tggtgctggg ccagtacgcc gccttcggca ccgacgtgaa gctgccgctg
      121 gccgatccca agagcgccgc cagcatgttc acccgcggca aggtttcgga tggctacccc
      181 tgcgccccgc tggcggtgac ggtcgcggcg ctggcccgca atccgcagga catcccgggg
      241 cggctgtgcc tgggcgattt cttccggatc aacgggctcg actatctgcg cggcgaattc
      301 gatcccgccc cgcggccgga cgagctgggc ggggcggcca agggctttgc cggcaagcgc
      361 aatttccgca gcgagttcta cacttcgatc attgccgatc ctcgcgccgg ggccaacgac
      421 aaggcctatg ccctctaccg cgcggtgatg tgctatgccc ctagcgccgt gaacgcgtgt
      481 ggcggggcca gcgtgcccaa gagccagcgc aaggcctggt ttgaccggct gaagcgcgaa
      541 tacccgcaaa gcgcctgggc taagaagctg cgctactact ggtgaggcgc ctcgcgctgg
      601 cacttgccct ggcgctggcc gcgtgcgcgc cgggcggatc gtccaccgtg cgggcggagg
      661 atcacgacgc gttcttcctg tgggccgggg tgcgcccgca gccggtgctg aaggaggcgc
      721 gcgagcttta cttgctggcc ggggaagtgc gccgcgacgg acgttatctg ccgctgcgcg
      781 ccgtgccccg cgccggtcag gccgagctgt ggctggtggt gcgggcgcaa agcctggact
      841 gggcccccgg cacgctggag cgggtcctgg ccgatctgga gcgctggcag gccgcgggca
      901 accggatggc cgggctgcag ctcgatttcg attcctcgac caaggggctg ggcggctatg
      961 ccctgtttct ggccgatctg cgcagccggc tggatccgca gttcaagctc tccatcaccg
     1021 gcctgatgga ctggggcgcc aatgccgatc cggcggcgct ggcgggcctc aagggcgtgg
     1081 tcgatgagat cgtggtgcaa agctatcagg gccgcgagac gatccccgga tacgaacact
     1141 atctggccag gctggcccgg gtgccggtgc cgcacaaggt ggcgctggtg cagggcggcg
     1201 aatggcggga gccggccgcg ctgaacgagg atccgctgtt ccgcggctat gtggtgtttc
     1261 tggtcaatcc cgacccgggc cgctagtggc tcaggccgcg gccgcgtggg gcgggcggca
     1321 atccgcatcg accgccggtt ccacccggtt gcggcccgaa ttcttggccc ggtaaagcgc
     1381 gccatcggcg gcgcgcagcg cggtgcgcgg atcgccgcag gcaaagacat cggccacccc
     1441 ggcggagaag gtcacctggc cgaacggttc gtcggtggcc cggttgacca gccgccgcgc
     1501 cgccaactgc tcgcgcaaca ggtccagctt ggcgacggcc tcggcctggg tgtcgccgcg
     1561 cagcagcagg acaaactcct cgcccccgtg ccgcgcgaca tggcagcggt cgttggagag
     1621 gtgggccagg cattcggcca ccagcttcag cacccggtcc ccggcctcgt ggccgtggcc
     1681 gtcgttgatc gccttgaaat ggtcgatgtc gcaaaaggcc acgcacagcg gctccatcgc
     1741 tgcgcgggcc tggcggtact gctcctcgaa cagggtttcg aaggcgcggc ggttgggcag
     1801 gccggtcagg tgatcgtgat cggcggtgtg gcgggcctcg tccagccggc ggcgcagggt
     1861 gcggctttgc gcctcgctgc gcagcatctg cttttccagc tcgcgcgagc ggcgcatcat
     1921 caccttggca atggtcgcca gctccgaaat cacgtcgccg gccttggtca cctgctccag
     1981 ctcgctgacg tggacttcca gcgcgctgtt gtattcgcgg gtcgcggtct gcgcatcgcg
     2041 gctggtccgg ccgaattcgt cgatcgagtt ttccagccgt tccatcagct gggccagcag
     2101 cgcggcatcg tcgccgttct gctggggccc cagttcctcg gccagccatt cggcggtcac
     2161 cggttcgcgc agctgcagcc ggcggtcaat cgcgcggacc acctggctgt ccgaaccagt
     2221 cagatagcgc caggcgatgg ccagcatgtc ggcgtcgacc tccagcttg
//
LOCUS       DASUDV010000088         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2013012,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000088 DASUDV010000000
VERSION     DASUDV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013012"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..197
                     /locus_tag="VFV30_01880"
     CDS             9..197
                     /locus_tag="VFV30_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846868.1"
                     /translation="MLINRIERFLRETGMPWTKFGRLATHDPRFVQDLRNGRTPRCET
                     EERVEHFMNTYRSQAHAH"
     gene            187..558
                     /locus_tag="VFV30_01885"
     CDS             187..558
                     /locus_tag="VFV30_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846869.1"
                     /translation="MHIDRRATAAMLCAAHRADRRTPWAALLKALLELAGNQAELISH
                     AERAWASVTFSGTRHHVRLRFVGAHSISAGERFIDALPDHEFSIPRQLVADAAVLSVT
                     NTVLPEPCLEVEVQLLLLEDA"
     gene            complement(568..1506)
                     /locus_tag="VFV30_01890"
     CDS             complement(568..1506)
                     /locus_tag="VFV30_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4846870.1"
                     /translation="MTIAVLNALLRPMMEGQLPQWLEPRWYETKEEALALAPEAEIGL
                     FDFYAREDVCAAIEQAVKLKWLNSLFAGVEKFPLALMQQRGVLLTNGAGINAVPIAEW
                     VVMGMLAIARGYREIVRAQERREWLRKAPGERLLMGSRALILGYGAIGQEVESRLAAF
                     GIEVSKVRRSPGAGVLGPDQWRARLGEFDWVIVSVPATAETEAMIGAAELAAMKRDAV
                     LVNVARGSVIDQQALTAALAEGRIGGALLDVTSPEPLPSEDALWGIERAHVTMHLSGH
                     SLQWGFQVAARRFVENLGRYQRGEALQPLVDYVRGY"
     gene            complement(1503..1961)
                     /locus_tag="VFV30_01895"
     CDS             complement(1503..1961)
                     /locus_tag="VFV30_01895"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX4846871.1"
                     /translation="MKLWRLTRAPFVALDGKGAEMHGARYAPPGHPVVPLASEAGLAV
                     LVALRYQPADLQGIPDDFVLGWTEADAEPIRVPDPDEDTIRTFMGEWLEAKASLLAAI
                     RSKVLPEADVIYMNPRHPDAARVPALTTRPFSFAECLHRPPMLDAFRGSS"
BASE COUNT          368 a          742 c          618 g          340 t
ORIGIN      
        1 ccttcctcat gctgatcaac cggatcgaac gcttcctgcg cgaaaccgga atgccgtgga
       61 ccaagttcgg gcggctcgcc acgcacgatc cgcgcttcgt ccaggacctg cgcaatggcc
      121 gcaccccgcg ttgcgaaaca gaagaacggg tggaacattt catgaacact tatcggagcc
      181 aggcccatgc acattgatcg ccgcgcgact gccgccatgc tttgcgccgc ccaccgcgcc
      241 gaccgccgca ccccctgggc cgcgctgctc aaggccttgc ttgagcttgc cggcaaccag
      301 gccgaactga tcagccatgc cgaacgggcc tgggccagcg tgacattttc gggcacccgc
      361 caccatgttc ggctgcgctt tgtcggggct cattcgataa gcgcgggcga acgcttcatc
      421 gatgccctgc ccgatcacga attctcgatt ccccggcaac tggtggccga tgcagcagtg
      481 ctgagcgtga ccaacacagt gctgcccgaa ccctgcctgg aggtggaagt ccagctgctg
      541 ctgctggaag atgcctaggg tcaggcccta gtaaccgcgc acgtaatcga ccagcggctg
      601 cagcgcttcg ccgcgctggt agcggcccag gttttcgaca aaccggcgcg ctgcaacctg
      661 gaaaccccat tgcagcgaat gacccgaaag gtgcatcgtc acatgcgcgc gttcgatccc
      721 ccacaaggca tcttcgcttg gcagcggctc gggactggtc acgtcgagca gggcgccgcc
      781 gatccggcct tcggccaggg ccgcggtgag cgcctgctga tcgatcaccg atccgcgcgc
      841 cacgttgacc agcaccgcat cgcgcttcat cgccgccagt tcggccgcgc cgatcatggc
      901 ttcggtttcg gccgtggcgg gaaccgagac gatcacccaa tcgaactcgc ccagccgcgc
      961 gcgccattga tcgggaccca gcacccctgc ccccggactg cggcgaacct tgctcacctc
     1021 gatcccgaag gcggcaagcc ggctttcgac ctcctggcca atcgctccgt aacccaggat
     1081 cagcgccctg ctgcccatca gcagacgctc accaggggcc ttgcgcagcc attcgcggcg
     1141 ctcctgcgcg cgcacgatct cgcggtagcc gcgggcgatc gccagcatgc ccatcaccac
     1201 ccattcggca atcggcacgg cgttgatccc cgcgccgttg gtcaacagca ccccgcgctg
     1261 ctgcatcaac gccagcggga acttttcaac cccggcgaac agcgaattga gccacttgag
     1321 cttgaccgcc tgctcaatcg cggcgcagac atcctcgcgc gcatagaaat cgaacaagcc
     1381 gatttcggcc tcgggcgcca gggccagcgc ctcttccttg gtttcgtacc agcgcggctc
     1441 aagccattgc ggaagctgcc cttccatcat ggggcgcaac aacgcgttga gaacggctat
     1501 ggtcatgacg aacccctgaa ggcatcgagc atcggcgggc ggtgcaggca ctcggcaaaa
     1561 ctgaacgggc gggtggtaag cgcgggcacg cgcgccgcat cgggatggcg cgggttcatg
     1621 tagatcacgt cggcttcggg cagcaccttc gatcggatcg cggcgagcag cgaggccttt
     1681 gcttcaagcc attcgcccat gaaggtgcgg atcgtatcct cgtcgggatc gggcacccgt
     1741 atcggctcgg catcggcctc ggtccagccg agcacgaagt catcgggaat gccctgcagg
     1801 tcggccggct ggtaacgcag cgccaccagc accgcgagcc cggcctcgct cgccagcggc
     1861 acaaccggat gcccgggcgg cgcatagcgc gcgccatgca tttcggcccc cttcccatcc
     1921 agcgccacaa acggcgcgcg ggtcaggcgc cagagtttca cgcttccagc ttccagtccc
     1981 agccgagcgg atcgccgtcc atcacctcca cgccttttgc ggcaaggtca tcgcgcagtg
     2041 catcggactt ggcaaagtcc ttcgcggc
//
LOCUS       DASUDV010000089         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_930612,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000089 DASUDV010000000
VERSION     DASUDV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930612"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFV30_01900"
     CDS             complement(<1..272)
                     /locus_tag="VFV30_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846872.1"
                     /translation="MKETCPECGLPLNAEGVCVNSACLASRVGAAPAGGGASAWTDPS
                     PRAGTGPAADSGGSTREDFDAAIKKLRDEVPKLKQAGVETEIITFCG"
     gene            complement(269..1828)
                     /locus_tag="VFV30_01905"
     CDS             complement(269..1828)
                     /locus_tag="VFV30_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846873.1"
                     /translation="MPTFALPTFLFGPNQGARNSTILERSGGSDDVPEAERRVLGDLP
                     LALAGWMGAREEDEIYGCLPLSRATTGGRLLFRAAYLRDVGGEPYGLAVGVYLPRELL
                     HELDGHAERLVDLLPWPDRDTRFVAAPLTLDTASLPALADGVPVGLEWDDRAVMVEDG
                     TPPLAVARTLLAGLRRPPQHRRVRGWVTTSRFPSLGGIDARSSFQLVVSAGSPPREDY
                     PTAIWRGEFRGPVSAPPPAWAAWQKLHALVADDRELAEASNWAANLAGLAPAAVAEGL
                     LARALSWPNTGPRLFLAMAHRFGLGGALADPAARIEETVFTVDAEQRGALISALVRMP
                     DPQRTTLGLWSLLARAPALAWETDPDTFALLLTELDWLGWMGRNGHTRFGTIEPEHRY
                     RAIEHGLAVLLADPRPDNPVYAPLKGLVLSLIGDNNSLVQSNPRNVAIARQVSAASEA
                     GLPLIDQRNWNFFENWARYLPKESLAPVRKQLYRVPPGLPADRDQLVLLTDLIAAVNR
                     AGRPSQQGFGR"
     gene            complement(1838..2449)
                     /locus_tag="VFV30_01910"
     CDS             complement(1838..2449)
                     /locus_tag="VFV30_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846874.1"
                     /translation="MDLDESEVARLRQEILAFLIHEMKAASANGELREAGAELAAPIA
                     ALVEEAVQRAIDRRPATRDGVLTEADLQTIARMVGTMPPPPRAADPSPAAVPDNGGHA
                     MRDLSQDIERARQADAPADSDGSTKAYAYGGIGLALGTALGVVIATLMGGDGPSGSAG
                     EPVERVAPTSTGVPTGIVPSGDGAVPPAAAPSSPAPSAPAKGP"
     gene            2626..3318
                     /locus_tag="VFV30_01915"
                     /pseudo
     CDS             2626..3318
                     /locus_tag="VFV30_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
BASE COUNT          507 a         1186 c         1147 g          478 t
ORIGIN      
        1 ccgcagaagg tgatgatctc ggtctccacc ccggcctgct tgagcttggg cacttcgtcg
       61 cgcagcttct tgatcgccgc gtcgaaatcc tcgcgggtgc tgccgccgct gtcggccgcc
      121 gggcccgttc cggctctggg cgaagggtcg gtccaggcgg aagcgccgcc gcccgcaggc
      181 gccgcgccaa cccggctggc caggcacgcg ctgttcacgc agaccccttc cgcgttcagc
      241 ggcaggccgc attccggaca ggtttccttc atcgcccaaa cccctgttgt gatggccggc
      301 ccgcccggtt caccgcggca atcagatcgg tcaggaggac cagctgatcg cgatcggccg
      361 gcagccccgg cggcacccgg taaagctgct tgcgcaccgg cgcgaggctc tccttgggaa
      421 ggtagcgagc ccagttttcg aagaagttcc agttgcgctg gtcgatcagc ggcagcccgg
      481 cctcggacgc ggcggagact tgccgggcaa tcgccacgtt gcgcggattg ctctggacca
      541 ggctgttgtt gtcgccaatc agcgacagga ccagcccctt gagcggggca tagaccgggt
      601 tgtccggccg gggatcggcc agcagcacgg ccagcccgtg ctcgatcgcg cggtagcggt
      661 gctccggctc gatcgtgccg aaccgggtgt gcccgttgcg gcccatccag cccagccagt
      721 caagctcggt cagcagcagc gcgaaagtgt ccggatcggt ttcccaggcc agggccggcg
      781 cgcgcgccag cagcgaccac agccccagcg tggtccgctg cggatcgggc atccgcacca
      841 gcgcagagat cagcgcgccg cgctgttcgg catcgacggt gaacaccgtc tcctcgatcc
      901 gggccgccgg atcggccagc gccccgccca gcccgaaccg gtgggccatg gccaggaaca
      961 gccgcggccc ggtgttcggc caggacagcg cccgggcgag cagcccttcg gccacggcgg
     1021 ccggggccag gcccgccaga ttggccgccc agttgctggc ctcggccagt tcgcggtcgt
     1081 cggccaccag cgcgtgaagc ttctgccagg ccgcccacgc cgggggcggc gcgctgaccg
     1141 ggccgcgaaa ttcgccgcgc cagatcgcgg tgggatagtc ctcgcgcggg ggcgatccgg
     1201 ccgacacgac cagttgaaag ctggagcgtg cgtcgatccc gcccaggctg gggaagcggc
     1261 tggtggtgac ccagccgcgc acccggcggt gctgcggcgg gcggcgcagc ccggccagca
     1321 gggtccgcgc cacggccagc ggcggcgtgc cgtcttccac catcaccgcg cggtcatccc
     1381 attccagccc caccggcacg ccatcggcca gcgccgggag gctggccgtg tcgagggtca
     1441 gcggggccgc gacaaagcgg gtgtcgcggt ccggccaggg cagcagatcg accagccgtt
     1501 cggcatggcc gtccagttcg tgcagcagtt cgcgcggcag gtagacaccc accgccaggc
     1561 catagggctc tccgccgaca tcgcgcagat aggcggcccg gaacagcagc cgcccaccgg
     1621 tggtggcgcg cgacagcggc aggcagccgt aaatctcgtc ctcttcgcgc gcgcccatcc
     1681 acccggccag ggcgagcggc agatcgccca gcacgcggcg ctcggcctcg ggcacgtcgt
     1741 cgctcccgcc cgaccgctcg aggatcgtgc tgttgcgcgc gccctggttg ggcccgaaca
     1801 ggaaagtggg aagcgcgaaa gtcggcatgg tgggcggtta cggccccttg gccggcgcgg
     1861 agggagccgg gcttgacggc gcggcggccg gcgggacggc gccatcgccg ctcggcacga
     1921 tcccggtcgg cacgccggtg ctggtcgggg cgacccgttc caccggctcg cctgccgatc
     1981 ccgacgggcc gtctccgccc atcagggtgg cgatcaccac gcccagcgcc gtgcccagcg
     2041 ccagcccgat cccgccatag gcataggcct tggtggaacc gtcgctgtcc gccggcgcgt
     2101 cggcctggcg ggcgcgttcg atatcctggc tcagatcgcg catcgcgtgg ccgccattgt
     2161 ccggcacggc tgcgggggac ggatcggccg cgcgcggcgg cggcggcatc gtgccgacca
     2221 tcctggcaat cgtctggaga tcggcctcgg tcagcacgcc gtcgcgggtt gccgggcggc
     2281 gatcgatcgc gcgctgcacc gcctcttcca ccagggcggc aatcggcgcg gccagctcgg
     2341 ccccggcctc gcgcagttct ccgttcgcgg aggcggcctt catttcgtgg atcagaaatg
     2401 caagaatttc ctgccgcaac cgggcaactt cggactcatc cagatccatt caggcgcact
     2461 cctcgatccc ggagaatcgt ttgcagtctt gacgtcagtg ccatgctagt atgcatcgca
     2521 agccgtgcaa cggatgaatc gcaagcctcg tccgcggccc tgtcacagca tgagactgcc
     2581 cccgcgcagc aaccaggaga gcccatgtcc gaccatcttc ccggcgccca ggaccatatc
     2641 gccgccgcgc tcaagctttg cgccaagatc gacgacattc cggccgacac cccgctgctg
     2701 ccgatcgcca aggtcggcgt gatcggcgcg ggcacgatgg gcggcgggat cacgatgaat
     2761 ttcctctccg ccggcattcc ggtgacgatc gtggagcagg aacaggccgc gctggaccgc
     2821 ggcgtgggcg tgatggccaa gaactacgaa gccagcgtca agcgcgggcg gattgaggcg
     2881 gccgacgccc aggccgcgat ggcccggctg acccccagcc tcagcttaga cgatctggcc
     2941 gattgcgacc tggtgatcga ggcggtctat gaatcgatgg aggtcaagaa ggacgtcttc
     3001 ggccggctcg acaagatcgt gaagccgggc gcgatcctgg ccagtaacac cagctacctc
     3061 gacatcgacg agatcgcgac cgcgaccacc cggcccgaag cggtgctggg gatgcacttc
     3121 ttctcgcccg ccaacatcat gaaactgctg gaagtggtgc gcggggccaa gaccggggcc
     3181 agccagcttg ccacggtgat gcagctggcc ggcacgatcc gcaaggtgcc ggtcgtctcg
     3241 ggcgtgtgcc acggcttcat cggcaaccgc atgctgtcca agcgccagga acaggccaac
     3301 gcgctcttga tggaaggc
//
LOCUS       DASUDV010000090         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1549125,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000090 DASUDV010000000
VERSION     DASUDV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549125"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFV30_01920"
     CDS             complement(<1..1077)
                     /locus_tag="VFV30_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4846875.1"
                     /translation="MSEAGASVAGQATPARQGGVWGLVLFMLLLHIVNQIDRQLVAAF
                     AADIMRDLELSRSQFALIAGLAFSGVYAFAALFAGVLADRLGRVRVLSGGLGIWSLFT
                     ALAGLAQGFWTMLAARPFVAAGEATLVPTASNIILARVGDRNKAAALGLFFAGIPLGI
                     GGGFLIAGWLGPLIGWRGTFLAMGALGIFALAVLVRLKDDPPDLSHRPTLGGNLAALM
                     AVFKAKPRALWASLAIIFAHFHTATSPFVQLWLTAEKGFDGRSAGQLYGLMFVVFGLL
                     GTFGAGLLGDWAKRRFGLDRAVTMFVLLLLLAPLLVLYRLAPGGTGLFLLGMAGSILF
                     MTVIYGPIFAIIEELLPARLKATST"
     gene            complement(1078..1902)
                     /locus_tag="VFV30_01925"
     CDS             complement(1078..1902)
                     /locus_tag="VFV30_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4846876.1"
                     /translation="MNWVSGRVCVVTGGARGLGRAIAAALGAAGGRLALCDVLADGLE
                     QTAAELRAAGCEVFAAPCDVSDSAAVDAFFAQVEQALGPVEVLVNNAALVPGGPEDEV
                     RRGRHYAYLTTPEPRTSLGFTSAMADAEWLKYWGVNVHGVFFCTRAALRQMEPRRYGR
                     IVNIASIAGLSALSAHSPHYSATKGAVIAFTRAVGAEVAGANIFVNAIAPGGVATPMF
                     DQYLASLDAEARGRLFQMIPAGRLGTMDEYAGLVLYLAGDGHYLAGQVISPNGGMV"
     gene            complement(1899..2957)
                     /locus_tag="VFV30_01930"
     CDS             complement(1899..2957)
                     /locus_tag="VFV30_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX4846877.1"
                     /translation="MHFLDDSLLPENQEKLVIQVAPYGPQWLPGDFPEDIPVSMADQV
                     QKAVDCYNAGATVLHLHCREPDGSGSKRLSLFNEMIDRIRTACPDMLIQVGGSISFAP
                     EGEGGEAKWLSDDTRHMLAELTPRPDQVTIAINTNQMDISALMSADDIAGTSLTNPAL
                     FETYRNMHYESGPAFVEEHLRRLVANGIQPHFMLGDLSQLETVERLIRKGHYNGPLVL
                     NYVAIGSSAAGTHPADLIEFARRVPDGAVLTIESVMRNVAPFNAIAIALGLHVRVGIE
                     DNLWSRKGERMTSVQQVEQMVRIANELHRDVATGPEAKAIYQIGTFYQSTDETLRRLG
                     YPPNRQPGQRGVPRWEYA"
BASE COUNT          526 a         1069 c         1039 g          461 t
ORIGIN      
        1 ggtcgacgtg gccttgagcc gggccggcag cagctcctcg atgatcgcga agatcgggcc
       61 gtagatcacc gtcatgaaca ggatcgaccc ggccatgccc agcaggaaca ggccggttcc
      121 gcccggggca agccggtaga gcaccagcag cggtgccagc agcagcaaga ggacgaacat
      181 cgtcaccgcc cggtccaggc caaagcggcg cttggcccaa tcgcccagca ggccggcccc
      241 gaaggttccg agcaggccaa agacgacgaa catcaggcca tagagctgtc cggccgagcg
      301 tccgtcgaag cctttctccg ccgtcagcca gagctgcacg aaggggctgg tcgccgtgtg
      361 aaaatgggcg aaaatgatcg ccagcgaggc ccacagggcg cggggcttgg ccttgaacac
      421 cgccatcagc gcggcaaggt tgccgcccag cgtcggccgg tgcgagagat cgggcggatc
      481 gtccttcagc cgcaccagca cggccagcgc gaagatcccg agggcgccca tggccaggaa
      541 cgtgccgcgc cagccgatca gcggcccaag ccacccggca atcaggaacc cgccgccgat
      601 ccccagcgga atcccggcaa agaacaggcc gagcgcggcg gccttgttgc ggtcacccac
      661 ccgcgccagg atgatgttgc tggcggtcgg caccagggtc gcttcccccg ccgccacgaa
      721 cggccgggcg gccagcatgg tccagaaccc ctgggccagc ccggccagcg cggtgaacag
      781 gctccagatg ccgagcccgc ccgacagcac cctgacccgc cccagccggt cggccagcac
      841 gcccgcgaac agcgcggcaa aggcatagac cccgctgaag gccagcccgg cgatcagcgc
      901 gaactggctg cggctgagct ccagatcgcg catgatgtcg gcggcaaagg cggcgacgag
      961 ctgccggtcg atctggttga cgatgtgcag cagcagcatg aacaggacca ggccccagac
     1021 cccgccctgc cgtgccggtg tggcctgacc cgcgacactc gcgcccgctt cgctcattca
     1081 gaccatcccc ccgttcgggc tgatcacctg gccggccagg taatgcccgt ctcccgccag
     1141 gtagagcaca agccccgcgt attcgtccat cgtcccgagc cggccagccg ggatcatctg
     1201 aaaaagccgc ccgcgcgcct cggcgtcgag gctggcgaga tactggtcga acatcggcgt
     1261 cgccaccccg cccggggcaa tcgcgttgac gaagatattg gccccggcca cctccgcacc
     1321 gaccgcgcgg gtaaaggcga tgactgctcc cttggtggcc gaataatgcg ggctgtgcgc
     1381 gctgagcgcc gaaaggccgg cgatcgaggc gatgttgacg atccggccat agcggcgcgg
     1441 ctccatctgg cgcagcgcgg cgcgggtgca gaagaacacc ccgtggacgt tgacgcccca
     1501 gtatttcagc cactccgcat ccgccatcgc gctggtgaaa cccagcgagg tgcgcggttc
     1561 cggcgtggtc aggtaggcat agtggcggcc gcggcggacc tcgtcctccg gcccgccggg
     1621 gaccagcgcg gcgttgttga ccagcacctc gaccggaccg agcgcctgct ccacctgggc
     1681 aaagaaggca tcgacggcgg cgctgtcgct gacatcgcag ggtgcggcaa acacctcgca
     1741 ccccgcggcg cgcagctcgg cggcggtctg ctccagcccg tccgccagca catcgcacaa
     1801 ggcgaggcga ccgccggccg cgcccagcgc agcggcgatc gcccgcccga gtccgcgcgc
     1861 accccccgtg acgacgcaga ctctcccact aacccagttc atgcatactc ccaccgcggc
     1921 acgccgcgct gaccgggctg gcggttgggc ggatagccca gccggcgcag cgtctcgtcg
     1981 gtcgactgat agaaggtgcc gatctggtag atcgccttgg cttccggccc ggtcgcgaca
     2041 tcgcggtgca gctcgttcgc gatccgcacc atctgttcga cctgctggac gctggtcatc
     2101 cgctcgccct tgcggctcca caggttgtcc tcgatcccga cgcggacgtg gaggccaagg
     2161 gcaatggcga tggcgttgaa cggggccacg ttgcgcatca ccgattcgat cgtcagcacg
     2221 gcgccatcgg gcacgcggcg ggcaaattcg atcaggtcag ccggatgggt cccggcggcg
     2281 ctgctgccga tggcgacata gttcagcacc agcgggccgt tgtagtgccc cttgcggatc
     2341 agccgctcga ccgtttccag ctggctgaga tcgcccagca tgaagtgcgg ctggatgccg
     2401 ttggcgacga ggcgccgcag gtgttcctcc acaaaggccg ggccgctttc gtaatgcatg
     2461 ttgcggtagg tttcgaacag cgccgggttg gtcaggctgg tcccggcgat gtcgtccgcg
     2521 ctcatcaggg cggagatgtc catctggttg gtgttgatcg cgatggtcac ctggtccggc
     2581 ctgggggtca gttcggccag catgtggcgg gtgtcgtcgg acagccactt ggcctcgccg
     2641 ccttcgcctt ccggtgcaaa gctgatcgag ccgccgacct ggatcagcat gtccgggcag
     2701 gcggtgcgga tgcggtcgat catctcgttg aacagcgaca gccgcttgga ccccgaaccg
     2761 tcgggctcgc ggcaatggag gtgcagcacg gtggccccgg cgttgtagca atcgaccgcc
     2821 ttctggacct ggtcggccat ggagacggga atgtcttcgg ggaaatcgcc cggcagccac
     2881 tgcgggccat agggggcgac ctggatcacc agcttttcct ggttttcggg cagcaggctg
     2941 tcatcgagga agtgcatggt tcagtcctcc ttgggctgcg gaacgggcat tccgcgggct
     3001 ttcatttcat cgaccagatg cggggtgccg agcgcgaccg agtggatccc cagcacggca
     3061 accagttgca gcacggccat gatctcttcg ggcgt
//
LOCUS       DASUDV010000091         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_820190,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000091 DASUDV010000000
VERSION     DASUDV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820190"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFV30_01935"
     CDS             complement(<1..342)
                     /locus_tag="VFV30_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEX4846878.1"
                     /translation="MIRRIVLIAGALVAGCQAEKATEPVGPDGFAYSASVEPQGSASL
                     QKVELPAAALIAFKRTDLGDVRIFDGRGKVLPIARLDAAGDDAQQTREVPLYPIAGTA
                     AAPGNPAVSIEL"
     gene            complement(339..3179)
                     /locus_tag="VFV30_01940"
     CDS             complement(339..3179)
                     /locus_tag="VFV30_01940"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX4846879.1"
                     /translation="MVWWAIAAGALIGMLTSYSLVGGAIVGGILGLGFGSWLQNILRR
                     EIDIAIERALGRADRATIPVENWDGTVTEVVVADEAPEPAPPEPIAAPVAERPEPALA
                     KPRPEPVWREPAPAQPSEEPIERAPTYAQTPSAIDDLSVRVREWFLGGNTIVRVGLVI
                     LFVGLVFLARLVAAAGLYPIEARLATVGLAGAALLAVGLWKRTARPDFALHMQGGGVA
                     VMYLTVFASARIYEVLPPGAAFAFMIAIATLGAVLAVTQNSLVMALASFLGGYGVPVL
                     LGGRAETPLGLFTYITVLNLAVLGIAWKKSWRSLNLLGFFATFAIAMLWGAGSYEDRH
                     FLICEVFLAITVAIYLATAVLYAHNTPGRLGNFADSTLLFGTAITGFGLQAAMVHDRP
                     YASAWSALAFGAAYLGVTAWTMRRKDPAMRLLNESMLAIGVGFVTMAVPLALDARWTS
                     AAWALEGAGAFWVGSRQARWMPRAFGMALQALAAVILLVNLGPTVSTTPLLNHGFFGP
                     LLIALPVLFTAWLLRRELPHSGSLPAQGWAPVEQGLRHAWFLGGFGFAALALLSEVTR
                     ELPTAAVQGWNEPAFETWQQAQLAMLALLGLMVLADWAGRRFAWRAALWPGRFSLSVI
                     VLAYLVALTTGRSVLYLPDLIAWTLAIGLHLWLLHRQDRAPQGIGRRWNAAMHTFGVL
                     LATAMLADGLFQLIDRADLWDSSWAGVVFLLASSGGVALLTRWAGPASTVDGEGWGWP
                     LAAHHRAYWWRAGAVLALLTFAGAASAAWFAEGVSDPLPYLPLLNPVDLSVGLALLAL
                     AWWRQMLGRARTQPMLAAALTGHGGLALGAGLGFVAVNGIWLRTAHHFMAVPWDYSTL
                     SHPTVLTGFSILWTLIAMGLMLFAHRRAMRLPWLAGASLLGVVVAKLLFVDMSQVEGF
                     ARIFAFIGVGLLMLLIGYFVPLPPRKGEEAKA"
BASE COUNT          631 a         1107 c         1037 g          430 t
ORIGIN      
        1 cagctcgatc gaaaccgcag ggtttccggg tgctgcagcg gtgccggcga tcgggtagag
       61 aggaacttcg cgggtttgct gcgcatcgtc gccagcagcg tcgagccgcg cgatcggcag
      121 cactttgccg cgcccgtcga aaatccgcac gtcgcccaga tcggttcgtt taaacgcgat
      181 caaggcggcg gcgggtaatt cgaccttctg caacgaagcg ctgccttgcg gttccacgct
      241 ggcggaatag gcaaaaccat cgggtccaac aggttcggtc gccttttcgg cctggcagcc
      301 agccaccagt gcgccggcga tcaggactat gcggcggatc atgcctttgc ctcctcgccc
      361 ttgcgcgggg gcaggggcac gaaataaccg atcagcagca tcagcaggcc caccccgatg
      421 aaggcgaaga tccgggcaaa gccttcgacc tggctcatgt cgacgaagag cagcttagcc
      481 accacaacgc ccagcaggct ggccccggcc agccagggca ggcgcatcgc ccggcggtgc
      541 gcgaacagca tcagccccat ggcgatcagc gtccacagga tcgaaaagcc ggtcagcacg
      601 gtggggtgcg agagggtcga atagtcccac ggcacggcca tgaagtgatg cgcggtgcgc
      661 agccagatcc cgtttaccgc aacaaagcca agccccgcgc ccagcgccag gccgccatgg
      721 ccggtcagcg ccgcggccag catcggctgc gtccgtgccc ggcccagcat ctgtcgccac
      781 caggccagcg ccagcagcgc aaggccaact gaaaggtcaa ccgggttgag caggggcaga
      841 tagggcaggg gatcgctcac cccttcggca aaccaggctg cgctggcggc gccggcaaag
      901 gtcagcagcg cgagaaccgc gccagcccgc caccaatagg cgcggtgatg tgcagcgagc
      961 ggccagcccc agccttcgcc atcgaccgtc gaggcagggc ctgcccagcg ggtgagcagc
     1021 gccacgccgc cgcttgatgc cagcaggaat accacgccgg cccacgagct gtcccacagg
     1081 tctgcccggt cgatcagctg gaacaggcca tcggccagca tggcggttgc cagcagcacg
     1141 ccgaaggtat gcatcgcggc gttccagcgg cggccgattc cttgcggcgc gcggtcctgg
     1201 cggtggagca accacaggtg cagcccgatc gccagcgtcc aggcgatcag atcgggcagg
     1261 taaagcacgc tccggccagt ggtgagcgcg acaagatagg ccagcacgat caccgacaga
     1321 ctgaaccgcc cgggccagag cgcggcgcgc caggcgaagc gccgcccggc ccagtcggcc
     1381 agaaccatca ggccaagcag cgcaagcatt gccaactgcg cctgctgcca ggtttcgaat
     1441 gccggctcgt tccagccttg caccgccgca gtcggcaatt cgcgcgtcac ttcgctcagt
     1501 agcgccagcg cagcaaagcc gaacccgccc aggaaccagg catggcgcag gccctgttcc
     1561 accggggccc agccctgagc cgggagagaa ccgctgtgcg gcaattcgcg gcgcagcagc
     1621 caggcggtga acagcacggg cagggcgatc agcaagggtc cgaagaagcc atggttgagc
     1681 aacggtgtgg tcgaaacggt cgggccaaga ttgaccagca ggattacggc ggccagcgcc
     1741 tgcaacgcca tgccgaaggc gcgcggcatc cagcgcgcct gccgcgatcc gacccagaac
     1801 gcgcccgcgc cttcgagcgc ccaggccgcg ctggtccatc tggcatcaag cgctagtggc
     1861 accgccatgg tgacgaagcc gacgccgatc gccagcatcg attcgttgag caggcgcatc
     1921 gccggatcct tgcgccgcat ggtccaggcg gtgacgccaa gataggccgc gccgaacgcc
     1981 agcgccgacc aggccgaagc atagggccga tcgtggacca tcgccgcctg caagccaaag
     2041 ccggtgatcg cggtgccgaa cagcagcgtt gaatccgcga aattgcccag ccttcccggg
     2101 gtgttgtggg catagagcac ggccgtggcg aggtagatcg caaccgtgat cgccagaaac
     2161 acctcgcaga tcaggaaatg tcggtcttca taggaacccg ccccccacag catggcgatc
     2221 gcgaaggtcg cgaagaagcc cagcaggttg aggctgcgcc agcttttttt ccaggcgatc
     2281 cccagaacgg ctagattgag cacggtgatg taggtgaaca ggccaagcgg cgtttcggcc
     2341 ctgccgccca gcagcaccgg cacgccgtag ccgcccagga aagaggccag cgccatgacc
     2401 agcgagttct gggtaaccgc cagcactgct cccagcgtgg caatcgcgat catgaaggca
     2461 aaagccgcgc ccggcggcag cacctcgtag atccgggccg aggcgaagac cgtgagatac
     2521 atgaccgcca cgccgccgcc ctgcatgtgc aacgcaaaat cggggcgggc ggtgcgcttc
     2581 caaaggccca cggcgagcag cgccgcaccg gccagcccga ccgtcgccag tcgagcctcg
     2641 atcggataga gcccggcggc ggctaccagc cgggcaagga acaccagccc gacgaacagg
     2701 atcaccagcc cgactcggac gatagtgttg ccgcccagga accattcgcg cacccggacg
     2761 gagagatcgt cgatcgccga gggagtctgg gcgtaagtcg gcgcccgctc gatcggctct
     2821 tcactcggct gtgccggtgc cggctcacgc cagacaggtt cagggcgcgg tttggccagc
     2881 gccggttccg gccgttccgc gacgggcgct gctatcggct cgggtggggc cggttcaggt
     2941 gcttcatcgg ccactacaac ctcggttacc gtgccgtccc aattttcaac cggtatcgtc
     3001 gcccggtcgg cccgacccaa tgcccgctcg atcgcgatgt cgatttcgcg gcgcagaatg
     3061 ttctgcaacc acgaaccgaa gcccagtccc agaatgccgc cgacgatcgc tccgccgacc
     3121 aggctgtacg aagtcagcat accgatcagc gcacccgccg cgattgccca ccagaccatc
     3181 ggtcactcct ccagcggttc tgtcc
//
LOCUS       DASUDV010000092         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1262037,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000092 DASUDV010000000
VERSION     DASUDV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262037"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFV30_01945"
     CDS             complement(<1..473)
                     /locus_tag="VFV30_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4846880.1"
                     /translation="MTAHDPSLTKNAGLLGWLGFGKKHEADQSPGEPAEAPVDRRENL
                     RRRQLDEVCSFLAYHQLEVSALTLSIAHNYLTGNDPNLVRLIDRQMQARQPVTLDWLD
                     EVLNRNERGEELAQLTQLMERLESSIEDFAKTSKEAQSATSEYHSALEEHVNELE"
     gene            complement(504..1463)
                     /locus_tag="VFV30_01950"
     CDS             complement(504..1463)
                     /locus_tag="VFV30_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX4846881.1"
                     /translation="MLRRALRLAAATFGLIAAAPAAHAWSEETHQTTGAIAWADLAQR
                     HPDSLARLLTIAAAHQDYRLFAPFAQGLGEPARERALFEWLARWPDDIRGGPEDFPKW
                     HYELRVGSGRTWAWPWTNGTASDGFAYNYAMLANGCAAPADRAKAIGWLLHIVGDIQQ
                     PLHGGHQMTEVYNLTDRAGSIAFVRRTAGGEPTDLHQYWDKMLELGAITPPGGGTDWA
                     NALLRLWPRQSLPEVSRSGDPGVLFSSYLDESATLAGMVAYQGTFLAASPDPALAPVV
                     SPAENAIAGQLAMRRVATSGYRIADILALALAEADRNQATCES"
     gene            complement(1474..2118)
                     /locus_tag="VFV30_01955"
     CDS             complement(1474..2118)
                     /locus_tag="VFV30_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX4846882.1"
                     /translation="MSWTDERIEKLTKMWEGGATASQIADELGGVSRNAVIGKAHRLG
                     LKARPSPVKPNEKEGAPPAAAPAPKAPRPEPAPRAAAPRPAAPAPAPRAAAPAPSGEP
                     MPRIVSVGPGGFLRQGPGDQQAPIPPAPPRRLVPAKPSPEVADKTSLLDLNDRICRWP
                     MGHPGEPDFHFCGEKVNPGFPYCVEHCGRAFQAQLPRGHRRPPPPLPFGGPRVR"
BASE COUNT          315 a          709 c          798 g          384 t
ORIGIN      
        1 tgttccagtt cgttgacatg ctcttccagc gcagaatggt attcgctggt cgcgctctgg
       61 gcttccttgc tggtcttggc gaagtcttcg atcgagcttt caagccgttc catcagctgg
      121 gtcagctggg ccagttcctc gccgcgttcg ttgcgattga gcacctcgtc gagccagtcg
      181 agcgtcaccg gttggcgggc ctgcatctgc cggtcgatca ggcggaccag gttaggatcg
      241 ttgccggtca gatagttgtg ggcaatcgac agggtcagcg cgcttacctc gagctgatga
      301 taggcgagga acgagcaaac ctcgtcaagc tggcgtcggc gcaggttctc gcggcggtca
      361 accggcgctt cagccggttc gccaggggat tgatcggcct cgtgcttctt gccgaagccc
      421 agccagccca gcaggccggc attctttgtc agagatggat cgtgcgcagt catgccaggt
      481 tgaacggcag gtcgcgggcg aatttaactc tcgcaggtcg cctggttgcg atcggcctcg
      541 gccagggcaa gggctaggat atcggcgatg cgatagccgc tggtggcaac ccggcgcatc
      601 gccagctggc cggcgatggc attttcggcc gggctaacca ccggcgccag cgcgggatcg
      661 ggcgaggcgg caaggaaggt gccctgatag gccaccattc cggccagggt cgcgctttca
      721 tcgaggtagc tggagaacag cacgcccggg tcaccgctcc ggctcacttc cggcaggctc
      781 tgccgtggcc atagccgcag cagcgcgttc gcccagtcgg tgccgccacc gggcggggtg
      841 atcgcgccca gttccagcat cttgtcccag tactggtgca gatcggttgg ttcgccgcca
      901 gcggttcgcc gcacgaaggc gatcgatccg gcccggtcgg tgaggttgta gacctcggtc
      961 atctggtggc cgccgtgcag cggttgctgg atatcgccga cgatgtggag cagccagccg
     1021 atcgccttgg cccggtcggc cggggcggcg cagccattgg ccagcatggc atagttgtag
     1081 gcaaagccgt cgctggcggt gccgttggtc cagggccagg cccaggtccg ccccgaaccc
     1141 acgcgcagtt catagtgcca cttgggaaag tcttcgggac cgccgcgaat gtcatcgggc
     1201 cagcgcgcca gccattcgaa cagcgcccgc tcccgcgctg gttcgccaag gccctgggcg
     1261 aagggtgcaa acaggcgata gtcctggtgc gccgccgcga tcgtgagcag cctggccagg
     1321 ctatcggggt ggcgctgggc cagatcggcc caggcgatcg ccccggtggt ctggtgggtt
     1381 tcttcggacc aggcgtgtgc ggccggcgct gccgcgatca acccgaaggt tgcagcggca
     1441 aggcgcagcg cccggcgcag catcccggcg ggctcagcga acccgcgggc cgccgaacgg
     1501 cagcggcggc ggcggacgcc ggtggccgcg cggcagctgg gcctggaagg cgcggccgca
     1561 atgctcgacg caatagggga agccggggtt caccttttcg ccgcagaaat ggaagtccgg
     1621 ctcaccgggg tgacccatag gccagcggca gatccggtcg ttaagatcga gcaggctggt
     1681 cttgtcggcc acttccgggc tgggcttggc gggcacgagg cggcgcggcg gcgcgggcgg
     1741 gatcggcgcc tgctgatcgc cagggccctg gcgcaagaag ccgccggggc caaccgagac
     1801 gatccgcggc atcggttcac ccgatggggc aggtgcggca gcgcgcggcg ccggtgccgg
     1861 cgcggccggg cgcggcgcag cggcgcgcgg ggcgggttcg gggcgcggtg ccttgggggc
     1921 aggggccgcg gccggcgggg cgccttcctt ttcgttcggc ttgaccgggc tgggccgcgc
     1981 cttcagccca aggcggtgcg ccttgccgat tacggcattg cggctgaccc cgcccagttc
     2041 gtcggcgatc tggctggcgg tggcgccgcc ttcccacatc ttggtcagct tttcgatccg
     2101 ttcgtcggtc cagctcatca atcaacctct ttgcacggcg ctcttggctc ttgcgcttgc
     2161 cacggcgagc gccagccgat actcggcaag ccatgtccga tcaaat
//
LOCUS       DASUDV010000093         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1527310,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000093 DASUDV010000000
VERSION     DASUDV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527310"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFV30_01960"
     CDS             <1..331
                     /locus_tag="VFV30_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX4846883.1"
                     /translation="ESGTLAKSASGALDSVPWVRVVNLARALDELAEAGYWRIGLDGE
                     GQSTLAEVLPTGPVALVLGAEGEGMRHNIVQHCDSIARLPITRAIESLNVSNAAAIAL
                     YAVATRA"
     gene            346..1230
                     /locus_tag="VFV30_01965"
     CDS             346..1230
                     /locus_tag="VFV30_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846884.1"
                     /translation="MNRPTAFRRFAAAGLAAAFSLLLSACLITPGKFDAALDLRKDGR
                     FTYSYKGEIFVLGLSELMKAANAAEAADAKFEPVPCYTDESMEKERPCSKDELAKQEA
                     DWRDERMDAANRQNKDLEMAKALLGGLDPTDPRAAEELAVRLRRQYGWTSVVYKGNGV
                     YVVDFRISGTLTHDFAFPTMEKMPQIAPFFIINRRNDGTVRVDTPGLSGEPNGMGTGF
                     GNLGQMLAAEKASRGGDKSLPGIPQIDGRFVLTTDGLILANNTDEGPKADPAGKRLEW
                     VITPREAAGPMALIELAK"
     gene            complement(1242..1916)
                     /locus_tag="VFV30_01970"
     CDS             complement(1242..1916)
                     /locus_tag="VFV30_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4846885.1"
                     /translation="MSSRIMQPKALAALGLAVALAAPALAQKETYPPAPLPADTYPPP
                     PTAVLSRPPVPTGNPGVWVQQSDYPPAALREEREGNTGFRLYVDATGRPTACTVTSSS
                     GSPDLDETACEMMMLRARFKPARDSKGLPTAGTFASRVRWVLPRDPEAYAPGERVLTF
                     YIQPDGLVTNCQETLNGEIVVPGQSGIRSPCIGGLRMTPPVDAAGKPVRKKVVIRQTL
                     TITDAE"
     gene            complement(1916..2641)
                     /locus_tag="VFV30_01975"
     CDS             complement(1916..2641)
                     /locus_tag="VFV30_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4846886.1"
                     /translation="MTIAGGLMVTLLAGAANAQDGSRSAPLPPPTETPRYVQPPAPAP
                     PLSQARGPVPANNPGEWIQTDDYPIRALREEREGTTAFRLTVGVDGLVTACTITGSSG
                     SPDLDSAACLNATRRARFKPAVGSDGRPAIGTYATRVNWVIPEDVEPGPQELAIPKHP
                     MPGQSVITYTIGIDGRAVDCQLVSGPDPADFLLWAMPCDYNQVFPVYTNAAGKAVPRK
                     VRLVLSVTLPTATAPAPKRKKRR"
     gene            2748..3974
                     /gene="argJ"
                     /locus_tag="VFV30_01980"
     CDS             2748..3974
                     /gene="argJ"
                     /locus_tag="VFV30_01980"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX4846887.1"
                     /translation="MAHAVSPLARPFPMLPPIGGVTLRVARAGYKDWGRCDLTYAELA
                     EGTAVAGVFTRNVCCSSEVELGRANVAQGRARALVVNAGNSNAFTGYRGREAVEQIMG
                     QVAAHLDCPPEQVFVSSTGVIGVPLPKDKARAGVAAALEAAPCGWEEAANTIGTTDTF
                     AKGAMAQAVVGGKTVTLAAIIKGSGMIAPDMATMLGYIFTDCAIAPGFLQQLLSEANQ
                     RTFSCITVDSDTSTSDTVLAFATGAAGNPELTCEDCPGADAFAAALEDVCRQLAHLVV
                     RDGEGAQKFIAVTVSGAVSDESARKVGLAIANSPLVKTAIAGEDANWGRVVMAVGKAG
                     EPADRDRLSIGFGGTWAAKDGQPLADYDEAPVAAHLKGQDISIEVDLGLGEGRATVWT
                     CDLTHGYISINADYRS"
     gene            4007..>4566
                     /locus_tag="VFV30_01985"
     CDS             4007..>4566
                     /locus_tag="VFV30_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4846888.1"
                     /translation="MQDAAARAILPRYQSLAAHEIDAKAADDVVTIADKEAELILAEG
                     LARLLPEAAIVGEEAAHADPSLLDRLGDALCWIIDPVDGTNNFAAGKPPFGIMVALSQ
                     AGETVAGWILDPLSGRLCHAHLGQGAFIGAERIAARGTGQAPPIAAISLVFMDPAKRE
                     AMKAHIAPHYSLVDIPRCAAEQYPRLV"
BASE COUNT          743 a         1586 c         1560 g          677 t
ORIGIN      
        1 cgaatcgggc accctggcca aaagcgcctc gggcgcgctg gactcggtgc cgtgggtccg
       61 cgtggtcaac ctggcccgcg cgctcgatga actggccgag gccgggtact ggcggatcgg
      121 gctcgacggc gaaggccagt cgaccctggc cgaggtgctg ccgaccggcc cggtcgcact
      181 ggtgctgggc gccgagggcg aaggcatgcg ccacaacatc gtccagcact gcgattcgat
      241 cgcccgcctg ccgatcaccc gcgcgatcga aagcctcaac gtatccaatg ccgccgcgat
      301 cgcgctctac gccgtcgcca cgcgggcctg aagggggaaa ccagcatgaa ccgtccaacc
      361 gcattccgcc gctttgccgc tgccggtctg gccgcggcct tcagtctgct gctctcggcc
      421 tgcctgatca ccccgggcaa gttcgatgcc gcgctcgatc tgcgcaagga cgggcgcttc
      481 acctattcct acaaaggtga gatcttcgtg ctgggcctgt cggagctgat gaaggccgcc
      541 aacgccgcgg aagcggccga tgcgaagttc gagcctgtgc cctgctacac cgacgagagc
      601 atggagaagg aacgcccctg ctccaaggac gaactggcca agcaggaagc ggactggcgg
      661 gatgagcgca tggatgcggc caaccggcag aacaaggacc tggaaatggc caaggcgctg
      721 ctcggcgggc tcgatcccac cgatccccgc gcggccgagg aactggccgt gcgcctgcgc
      781 cgccagtacg gctggaccag cgtggtctac aagggcaacg gcgtctacgt ggtcgacttc
      841 cggatcagcg gcaccctgac tcacgacttc gcgttcccga ccatggaaaa gatgccccag
      901 atcgccccgt tcttcatcat caaccgccgc aatgacggca cggtgcgggt cgacaccccg
      961 ggcctgtccg gcgaaccgaa cggcatgggc accggctttg gcaacctcgg ccagatgctg
     1021 gccgccgaaa aggccagccg cggcggcgac aagagcctgc ccggcattcc ccagatcgac
     1081 ggccgtttcg tgctgaccac cgacggcctg atcctggcca acaacaccga cgaaggcccc
     1141 aaggccgatc cggccggcaa gcggctggaa tgggtcatca ccccgcgtga agccgccggg
     1201 ccgatggcgc tgatcgaact ggcgaagtaa ccgggggggc gctattcggc gtcggttatc
     1261 gtcagggtct ggcggatcac cactttcttg cggaccggct tgccggccgc atcgactggc
     1321 ggcgtcatgc gcaggccccc gatgcaggga ctgcggatgc ccgactggcc gggtacgaca
     1381 atctccccgt tcagggtctc ctggcagttc gtcaccaggc catcgggttg gatatagaag
     1441 gtcagcaccc gctcgcccgg cgcgtaggcc tccggatcac gcggcagcac ccaccggacc
     1501 cggctggcga aggtgcccgc ggtgggcagg cccttgctgt cccgcgccgg cttgaaccgc
     1561 gcgcgcagca tcatcatttc gcaggccgtt tcgtccaaat cgggcgagcc gctggagctt
     1621 gtaacggtgc aagccgtcgg ccggccggtt gcatcaacgt acagccggaa accagtgttg
     1681 ccttcgcgct cctcgcgcaa ggccgctggc ggatagtcgc tctgctgcac ccacacgccc
     1741 gggttccccg tgggcacagg cggccgggac agcaccgccg tgggcggcgg agggtaagtg
     1801 tcggcaggca agggcgcagg cggataggtt tccttctgcg cgagcgccgg tgcggcgaga
     1861 gccacggcga gccccaaagc ggcaagtgcc tttggttgca tgatccggct cgacatcagc
     1921 ggcgcttctt gcgcttgggt gccggcgcgg tggcggtcgg cagggttacg ctcagcacca
     1981 gccggacctt gcgcggcacc gccttgccgg ccgcgttggt gtaaaccggg aagacctggt
     2041 tgtaatcgca cggcatggcc cagagcagga aatcggccgg atcgggcccc gaaacgagct
     2101 ggcaatcaac ggcgcgcccg tcgatcccga tggtgtaggt gatcacgctt tgccccggca
     2161 tcgggtgctt ggggatcgcc agttcctgcg gtcccggctc aacatcctcg ggaattaccc
     2221 agttcacccg ggtcgcgtaa gtgccgatgg ccggccttcc atcgctgccg accgctggct
     2281 tgaaccgggc gcgccgagtc gcattcaggc aggctgccga gtcgagatcg ggcgagcccg
     2341 acgagccggt gatggtgcaa gcagtcacca ggccgtccac gcccacggtc agccgaaacg
     2401 cggtggtccc ttcccgctcc tcgcgcaagg cgcggatggg gtaatcgtca gtctggatcc
     2461 attcgccggg gttgttggcc ggaaccgggc cgcgggcctg actgagcggc ggcgcagggg
     2521 ccggcggctg tacatagcgc ggggtctccg tcggcggcgg aagcggcgcg gaccttgaac
     2581 cgtcctgcgc attcgccgcg ccggccagca gcgtgaccat cagaccgccc gcgattgtca
     2641 ttgcccgcat ccgatttcct ccctcaaaca agcccttgca gcctatcagg tgcagccgcg
     2701 cccgcaaccc acttgccccg cgcccgcccg aatcctaagg ccggggcatg gcccatgcag
     2761 tctcaccgct cgcccgcccc ttccccatgt tgccgccgat cggcggggtg acgctgcgcg
     2821 tggcgcgcgc cgggtacaag gattgggggc gctgcgacct gacctatgcc gaactggccg
     2881 agggcacggc cgtggccggg gtcttcaccc gcaacgtctg ctgctccagc gaggtggaac
     2941 tgggccgcgc caacgtggcg caaggccgcg cccgggcgct ggtggtcaac gccggcaatt
     3001 ccaacgcctt caccggctat cgcgggcgtg aggcggtgga gcagatcatg ggccaggtcg
     3061 cggcgcacct cgactgcccg ccagagcagg tgttcgtttc ctccaccggg gtgatcggcg
     3121 tgccgctgcc caaggacaag gcccgggccg gcgtcgcggc cgcgctggag gcagcgccct
     3181 gcggctggga agaggccgcc aacaccatcg gcaccaccga tacctttgcc aagggcgcga
     3241 tggcgcaggc cgtagtcggc ggcaagaccg tcaccctggc cgcgatcatc aagggcagcg
     3301 ggatgatcgc gcccgacatg gcgaccatgc tgggctacat cttcaccgat tgcgcgattg
     3361 cccccggctt tctccagcaa ctgctgagcg aggccaacca gcgcaccttc agctgcatca
     3421 cggtggacag cgacacctcc accagcgaca cggtgctggc ctttgccacc ggcgcggccg
     3481 gcaaccctga gctcacttgc gaagactgcc ccggcgccga tgcctttgcc gccgcgctgg
     3541 aagacgtctg ccgccagctg gcccacctgg tggtgcgcga cggcgaaggc gcgcagaagt
     3601 tcatcgcggt caccgtgagc ggcgcggtca gcgacgagag cgcgcggaaa gtgggcctcg
     3661 ccatcgccaa ctccccgctg gtcaagaccg cgattgccgg ggaagacgcc aactggggcc
     3721 gcgtggtcat ggccgtgggc aaggccgggg aaccggcgga ccgcgacagg ctctcgatcg
     3781 ggttcggcgg cacctgggcc gcgaaggacg gacagccgct ggccgattac gacgaggccc
     3841 cggtcgccgc gcacctcaag gggcaggaca tcagcatcga ggttgacctg ggcctgggcg
     3901 aaggccgcgc caccgtgtgg acctgcgatc tgacccacgg ctacatctcg atcaacgcgg
     3961 actaccgctc ttgacccagg ccctgaccag cggggtcctc gctatcatgc aggatgccgc
     4021 cgcgcgcgcg atcctgccgc gctaccagtc gcttgccgca cacgagatcg atgccaaggc
     4081 ggccgacgac gtggtgacca ttgccgacaa ggaggccgag ctgatcctgg cggaggggct
     4141 ggcccgcttg ctgcccgagg ccgcgatcgt cggcgaagag gccgcccacg ccgatccctc
     4201 cttgctggac cggctgggcg atgcgctgtg ctggatcatc gatccggtcg acggcaccaa
     4261 caatttcgcg gccggcaagc cgccgttcgg gatcatggtg gcgctgagcc aggcgggcga
     4321 aacggtcgcc gggtggatcc tcgatccgct cagcgggcgg ctgtgccatg cccacctggg
     4381 ccagggcgcc ttcatcggcg cggagcggat cgcggcgcgc ggcaccggcc aggcaccgcc
     4441 gatcgcggcg atcagcctgg ttttcatgga cccggccaag cgcgaggcga tgaaagccca
     4501 catcgcgccg cattacagcc tggtcgacat cccgcgctgc gcggcggagc agtacccccg
     4561 gctggt
//
LOCUS       DASUDV010000094         5639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1637592,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000094 DASUDV010000000
VERSION     DASUDV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637592"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..183
                     /gene="ykgO"
                     /locus_tag="VFV30_01990"
     CDS             58..183
                     /gene="ykgO"
                     /locus_tag="VFV30_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX4846889.1"
                     /translation="MKIRNSLKSLKDRHRDNRVIRRRGRTYVINKTNRRFKARQG"
     gene            291..896
                     /locus_tag="VFV30_01995"
     CDS             291..896
                     /locus_tag="VFV30_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX4846890.1"
                     /translation="MVEAVVFDVGKVLVEWERDLPFRDLIPDPAERAWFMETVIPLAW
                     HGEHDAGRDAEEMIAARSAEYPDHAHLIRAWLDRFNDTIPGPVPGSVELVEALDAAGV
                     PLYAITNFGADTWAGFAPTFPLFERFRDIVVSGVEKLAKPDPAIFALAEARFGLAPQT
                     MLFIDDSLPNVDSARARGWHGHHFTGAAGLERDLIAHGLLG"
     gene            complement(985..1332)
                     /locus_tag="VFV30_02000"
     CDS             complement(985..1332)
                     /locus_tag="VFV30_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846891.1"
                     /translation="MMTLTNSHSVSRTLLLAGLALVGTVASFSATVSPAQAAGQRYTA
                     KLATPLAAPAKKVVNGVVWNCTGDTCTGAVDGARPLNTCARVVKAFGPVTSFATPKGE
                     FSADDVQSCNAAA"
     gene            1528..2043
                     /locus_tag="VFV30_02005"
     CDS             1528..2043
                     /locus_tag="VFV30_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4846892.1"
                     /translation="MGKLREPLNDMQNCGLPAALEVMGERWSFMILRAAFNGLHHFEE
                     FLGALGIARNILSNRLARLVEHGIMKREHMPDDRRKIEYRLTEKGLDLLPAMLALRQW
                     GEKYGVGVPSNPVLCDERDGQPIALIGIHAHDGRVLGPMDLEWREAADVGKARVDYKD
                     EVVRPDLRALN"
     gene            complement(2045..3553)
                     /locus_tag="VFV30_02010"
     CDS             complement(2045..3553)
                     /locus_tag="VFV30_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX4846893.1"
                     /translation="MVALVQTVAYLGLEARSVEVQCQIAPGLPRFNVVGLPDKAVNES
                     KERVGAALSAMGLSLPPKRITINLSPADLPKEGSHYDLPIALALLAAMGVTDAEQLGD
                     FVAVGELALDGRIVASPGVLLAALHASAQDCSLICPAVQGSEAAWASGVAVVPAPNLI
                     SLLNHLKGTQQLPQPKPGEAEEPGFGPDLKQVKGQEVAKRALEIAAAGGHNLLMIGPP
                     GAGKSLLASCLPGILPPLTPAEALEVSMVASVAGTLEEGRISRSRPFRSPHHSASMAA
                     LTGGGLRVRPGEVSLAHLGVLFLDELPEFQRAVLDSLRQPLETGVVDVARANAHVTFP
                     ARVQLIAAMNPCRCGHLGDPALGCSRAPRCAADYQSKVSGPMLDRIDLHVEVDPVSAA
                     DLALPPPAEGSAEVAARVASARAVQTARMAGTGKRSNAELDGDLLEAHATPDEPGRKL
                     LLQAAEAMRLSARGYTRMLRVARTIADLAGSEGVGRIHVAEALSYRRVAPRA"
     gene            3702..4388
                     /locus_tag="VFV30_02015"
     CDS             3702..4388
                     /locus_tag="VFV30_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4846894.1"
                     /translation="MRRIVILLLSLFAMAVPAHAGETVTYEVVEETVEVTEVVDEGGS
                     ERFVSVAPAPIPQGIAQFGPFRVLDGTRAALVDVTDSASPAQFEAMMRAFPGISMIEM
                     IEVPGTEDDRANLKLGRMIRARGLTTYVPAGGSVRSGGVELFLAGKNRIADPGSEFAV
                     HAWMDEDGREATDYPANAPENRAYVDYYVEMGMPEGQARAFYAMTNSVRHNDAKWLTA
                     ADIGQWVNLN"
     gene            4493..4627
                     /gene="rpmH"
                     /locus_tag="VFV30_02020"
     CDS             4493..4627
                     /gene="rpmH"
                     /locus_tag="VFV30_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX4846895.1"
                     /translation="MKRTFQPSNLVRARRHGFRARTATVGGRKVLRARRARGRKKLSA
                     "
     gene            4639..4992
                     /gene="rnpA"
                     /locus_tag="VFV30_02025"
     CDS             4639..4992
                     /gene="rnpA"
                     /locus_tag="VFV30_02025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX4846896.1"
                     /translation="MMSVLTKRADFLAANRGLRIARPGFVLLVNPNGTDAVRYGITVT
                     KKIGNAVVRNRMKRRFRALLREALPTAGLPAHDHVLIGREGGVERDFAKLREELAMAL
                     SRAAQGKGDPPRKRK"
     gene            4989..5198
                     /gene="yidD"
                     /locus_tag="VFV30_02030"
     CDS             4989..5198
                     /gene="yidD"
                     /locus_tag="VFV30_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX4846897.1"
                     /translation="MKQLLILIARGWQLGPSRVLPPSCRYAPSCSQYAIEAVEKHGAI
                     KGGWLAFKRIMRCHPWGGHGYDPVP"
     gene            5256..>5639
                     /gene="yidC"
                     /locus_tag="VFV30_02035"
     CDS             5256..>5639
                     /gene="yidC"
                     /locus_tag="VFV30_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4846898.1"
                     /translation="MDNQNSRNMILAGILTVTILLGWDQAMRYFYPQANAPKPAATAS
                     SAPTPAASGGAKKTREGGLTNPADIALEAQDIKVALANPARVKIEAPGLSGSINLAGG
                     VIDDLSTNRHTAALDQSQGLQRIYSP"
BASE COUNT          938 a         1947 c         1873 g          881 t
ORIGIN      
        1 tcgcccctaa cggcgcacct tcaaatttca gcggcatcgg ccgccccgag gaaacccatg
       61 aagattcgca acagcctcaa gtcgctcaag gaccgccacc gggacaaccg cgtgatccgc
      121 cgccgtggcc ggacctatgt gatcaacaag accaaccgcc gcttcaaggc gcgccagggc
      181 taaacccctg acggaacggg tccgccgcgg cgcggccccg gcaatctgac ggcccgcctc
      241 ccatccgggg tgcgggccga aagtgttttt accgcaggag tgcgcagtcc atggtcgaag
      301 cagtcgtgtt cgatgtcggc aaggtcctgg tcgaatggga acgcgatctg ccgttccgcg
      361 acctgatccc cgatccggcc gagcgcgcct ggttcatgga aacggtgatc ccgctggcct
      421 ggcacggcga gcacgacgcc gggcgcgacg ccgaagagat gattgccgcc cgcagcgccg
      481 aatacccgga ccacgcccac ctgattcgcg cctggctgga ccgcttcaat gataccattc
      541 ccggcccggt gcccggatcg gtggagctgg tcgaggcgct cgacgcggcc ggggtgccgc
      601 tttacgcgat caccaatttc ggggccgaca cctgggccgg ctttgccccc accttcccgc
      661 tgttcgaacg gttccgcgac atcgtggtct cgggcgtgga aaagctggcc aagcccgatc
      721 cggcgatctt tgccctggcc gaggcccgct ttggcctggc gccgcagacc atgctgttca
      781 tcgatgacag cctgcccaac gtggacagcg cccgcgcccg cggctggcac ggccaccact
      841 ttaccggtgc cgccggactg gaacgcgatc tgatcgcgca cggcctgctg ggctgagcgc
      901 cgggcgctgc ccaaaaaaga ggcccccgac cttgcgggcg ggggccaatt tgaggggggc
      961 tgttaagggt gcctggattg cgcctcaggc ggcggcgttg cagctctgaa cgtcgtcagc
     1021 gctgaattcg cccttcgggg tggcgaaaga ggtcaccgga ccgaaggcct tgacgacccg
     1081 ggcgcaggtg ttgagcgggc gggcgccatc gaccgcgccg gtgcaggtgt cgccggtgca
     1141 gttccagacc acgccgttga caaccttctt ggccggagcc gcgagcgggg tcgccagctt
     1201 ggcggtgtag cgctggccgg cggcctgggc cgggctgacg gtggcggaga agctggccac
     1261 ggtgccgacg agggccaggc cggcgagcag cagggtgcgc gaaacgctgt gcgaattggt
     1321 caaagtcatc attctaaact cccctttggg tcatgcacgc cgcaatcgga ggggtcctgg
     1381 gagggaaggc tcgatttgcg ttgcgaatca ctacctatta cgataggtat taagttgcaa
     1441 ctcaaaactg tgcagtgcgg caaagatttt tttgtcggtc cgcaagcgag attgggttac
     1501 agtcccggtg ggaatgggga acgactgatg ggtaagctgc gcgaaccgtt gaacgacatg
     1561 cagaactgcg gccttccggc cgcgctggaa gtgatgggcg aacgctggag cttcatgatc
     1621 ctgcgcgccg ccttcaacgg cctgcaccat ttcgaagagt ttctgggggc gctggggatt
     1681 gcccgcaaca tcctctccaa ccgcctcgcg cggctggtcg aacacggcat catgaagcgc
     1741 gagcacatgc ccgacgaccg ccgcaagatc gaataccgcc tgaccgaaaa ggggctggac
     1801 ctgctccccg ccatgctcgc cctgcgccag tggggcgaaa agtacggggt cggcgttccc
     1861 agcaacccgg tgctgtgcga cgagcgcgac ggccagccga tcgcgctgat cggcatccac
     1921 gcccacgacg gccgcgtgct gggcccgatg gacctggaat ggcgcgaggc cgccgatgtc
     1981 ggcaaggcgc gggtggacta caaggacgaa gtcgtccgcc cggatctgcg cgcgcttaac
     2041 tagtctaggc ccgcggcgcc acccggcggt agctcagcgc ttcggccacg tggatccgcc
     2101 ccaccccctc gctccccgcc agatcggcaa tggtccgcgc cacccgcagc atccgggtgt
     2161 agccgcgcgc cgagagccgc atcgcttcgg ccgcctgcaa caacaacttg cggcccggct
     2221 cgtccggggt ggcgtgggct tccagcaggt cgccgtcgag ctcggcattg ctgcgcttgc
     2281 ccgtccccgc catccgcgcg gtctggacgg cgcgggcgga ggcgacccgg gcggccactt
     2341 cggcgctgcc ctcggccggg ggcggcaggg cgagatcggc ggcgctgacc ggatcgactt
     2401 cgacatgcag gtcaatccgg tccagcatcg ggcccgaaac cttgctttgg taatcggcgg
     2461 cgcagcgcgg cgcgcgggag cagcccagcg ccggatcgcc caggtgcccg cagcggcacg
     2521 ggttcatcgc cgcgatcagc tggacccggg ccggaaaggt cacatgggcg ttggcccggg
     2581 ccacatcgac caccccggtt tccagcggct ggcgcagcga atcgagcacc gcgcgctgaa
     2641 actccggcag ctcatcgagg aacaggacac ccaggtgcgc caggctgacc tcccccgggc
     2701 ggacccgcag tccgccgccg gtcagcgccg ccatcgaggc cgaatggtgg ggtgatcgga
     2761 acggccggga gcggctgatc cgcccctcct ccagcgtgcc ggcgaccgag gcgaccatcg
     2821 acacttccaa ggcctcggcc ggggtcagcg gggggaggat gccgggcagg cagctggcca
     2881 gcaggctttt ccccgcgccg ggcgggccga tcatcagcag gttgtgcccg ccggccgcgg
     2941 caatctccag cgcgcgcttg gccacttcct ggcccttcac ttgcttgagg tccggcccga
     3001 agcccggctc ctccgcctcg cccggtttgg gctggggcaa ctgctgcgtg cctttcaggt
     3061 gattgagcag gctgatcagg ttgggcgcgg gcaccacggc cacgccgctg gcccaggccg
     3121 cctcgctccc ctgcacggcc gggcagatca ggctgcaatc ctgcgcgctg gcgtggagcg
     3181 cggcgagcag cacgcccggc gaggcgacga tccgcccgtc gagcgccagt tcccccaccg
     3241 ccacgaaatc gcccagctgt tcggcatcgg tcacccccat cgcggccagc agggccagcg
     3301 cgatcggcag gtcatagtgg ctgccttcct tgggcaggtc ggccggggac aggttgatcg
     3361 tgatccgctt gggcggaagc gacaggccca tggccgaaag cgccgcgccg acccgttcct
     3421 tgctttcgtt caccgccttg tcgggcaggc caaccacgtt gaaacgcggc aggccgggtg
     3481 cgatctggca ctgcacttcg acgctgcgcg cctccagccc caagtaagcg acagtctgaa
     3541 ccagcgcgac caagtaatgc ccctgtgcgg attcgtacga atacttgggt gatggcctgt
     3601 tgttcagagc ttgtcgagcc gattgcacca aagcggcggt aaccaatcct taaccggctg
     3661 cattggcatt ccggcaagtg cgcggggccg ataggatggc catgcgccgc atcgtcatcc
     3721 tgctgctgtc cctgttcgcc atggccgtcc cggcccatgc cggcgagacg gtgacctatg
     3781 aagtggtcga ggaaacggtc gaggtcaccg aagtcgtcga cgagggcggc tcggaacgct
     3841 tcgtcagcgt cgctccggcg ccgatcccgc aggggatcgc gcagttcggg ccgttccgcg
     3901 tgctcgacgg gacccgcgcc gcgctggtcg acgtcaccga cagcgccagc ccggcgcagt
     3961 tcgaggcgat gatgcgcgcc ttccccggca tttcgatgat cgaaatgatc gaggttcccg
     4021 gcaccgagga cgaccgcgcc aacctgaagc tgggccggat gatccgcgcc cgtgggctca
     4081 ccacctatgt cccggccggc ggatcggtcc gttcgggcgg ggtcgagctg ttcctggccg
     4141 gcaagaaccg gatcgccgat cccggcagcg agttcgcggt tcacgcctgg atggacgagg
     4201 acggccgcga ggcgaccgac tatcccgcca acgcgcccga gaatcgcgcc tatgtcgatt
     4261 actatgtcga aatgggcatg cccgaaggcc aggcccgcgc cttctatgcc atgaccaatt
     4321 cggtgcggca caacgacgcc aagtggctga ccgccgccga catcggccaa tgggtgaacc
     4381 tgaactgatc cggccccgcc gaatcccttg actcacccgc ccggccctag taacggcccg
     4441 cgtctgatcg cggggcttcc cgcaaacaca cagatttccg aaggttcttg aaatgaagcg
     4501 cacgttccag cccagcaacc ttgttcgcgc ccgccgccat ggcttccgcg cccgcaccgc
     4561 aaccgtcggt ggccgcaagg tcctgcgcgc ccgccgcgcc cgcggccgca agaagctctc
     4621 ggcctgagcc acagtccaat gatgtcggtc ctgacgaaaa gggccgattt ccttgcagcc
     4681 aaccggggcc tgcggattgc gcgccccggt tttgtgcttt tggtgaaccc gaacgggacc
     4741 gacgcggtcc gctatggcat caccgttacc aagaagatcg gcaacgcggt ggtccgcaac
     4801 cggatgaagc gccggttccg cgccctgctg cgcgaggcgc tgcccaccgc cggcctgccc
     4861 gcccatgatc acgtgctgat cggccgcgaa ggcggggtgg agcgcgattt cgccaagctg
     4921 cgcgaggaac tggccatggc cctctcccgc gcggcccagg gcaagggcga cccgccccgg
     4981 aaacgcaagt gaaacagctc ctcatcctga tcgcccgcgg ttggcagctg gggccctcgc
     5041 gggtcctgcc gccctcgtgc cgctatgccc cctcgtgctc gcaatacgcg atcgaggcgg
     5101 tggagaaaca cggtgcgatt aagggtggct ggttggcctt caagcgtata atgcgctgcc
     5161 atccgtgggg agggcacggt tacgacccgg tgccttgacc ggggccgaac cttccccatc
     5221 tgacctagaa cgcattttcc ttacaggggc cctcagtgga caaccagaac agccgcaaca
     5281 tgatcctggc cgggattctg actgtcacga tcctgctcgg ctgggatcag gcgatgcggt
     5341 atttctatcc gcaggccaat gcccccaagc cagctgccac ggccagctcc gcccccaccc
     5401 cggcggcaag cggcggcgcc aagaagaccc gcgagggcgg gctgaccaac ccggccgaca
     5461 tcgcgctgga agcccaggac atcaaggtcg cactggccaa tccggcgcgg gtcaagatcg
     5521 aggcgccggg cctgtccggc tcgatcaatc tggccggcgg ggtgatcgac gatctcagca
     5581 ccaaccgcca caccgccgcg ctcgatcaga gccagggcct gcagcggatc tattcgccg
//
LOCUS       DASUDV010000095         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2167806,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000095 DASUDV010000000
VERSION     DASUDV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167806"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFV30_02040"
     CDS             <1..972
                     /locus_tag="VFV30_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX4846899.1"
                     /translation="PLINMASIGGITMSWLKVVGTMVLHHPFDPQVYLRQIATERPGF
                     TIAPPAVLNMLLQNEALLASVDLSSLHSIGSGSAPLAPAMVKGFQDRFAIMVINLFGS
                     NEGMSLVTGPDDVPDPEERASYFPRRPLFEPPCGGEPARHLETRLVAPGCGGEITEPG
                     VPGELQIRGPTVFEGYWRASEQTAAAFTEDGFFKTGDLFQIEGEGRFYLFVGRCKDLI
                     IRGGVNIAPEEIDQLLGGHPDLAEACVFSLPDPVLGERVGLAAVPRDGRALTLEDVTG
                     FLRRHDLAVFKLPERLFVFDSLPRNVTNKVMRGEVREMALKLMEQGG"
     gene            975..2207
                     /locus_tag="VFV30_02045"
     CDS             975..2207
                     /locus_tag="VFV30_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX4846900.1"
                     /translation="MAAEVFVLGGAQSDFARNLDREGGGMFELFRDVAEAAFAATGIE
                     PAEVEVAHVGNFVGELFAGQGQLGGFFGHVHPDLAGVPAARHEAACASGSIALLAAGA
                     EIEAGRYGLALVLGIELMRNVPGQRAAEYLGSAAWAGREAQEARYLWPYMFSQVAQEY
                     SERFGLDRAHLNAISQIAFANAKRNPNSQTRGWAITPEHLSDANDELNPPIEGSLRKA
                     DCGQVTDGAAAVFLASRAVAERYAARRGIALESIPRIKGWGHTTAPLLYSTKVSASRG
                     QPYVFPSVRKAMMDALGRAGMADVYACDGVEVHDCFSITEYMAIDHFGITAPGESWKA
                     VEDGTIALGGRLPVNPSGGLIGLGHPVGATGVRMMLDAWRQVTGNAGDYQVEGARNFA
                     TFNVGGSATTSVSFVVGV"
     gene            2215..>3142
                     /locus_tag="VFV30_02050"
     CDS             2215..>3142
                     /locus_tag="VFV30_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thiolase"
                     /protein_id="HEX4846901.1"
                     /translation="MEEVYLYDALRTPRGKARADGGLAGLTPPELVGVLIDGLRERTG
                     DACANPGALLLGCVTQSGAQGGHVAMAAKQYSGLPDDCAAQTLNNYCSSGLSAIGHGV
                     AMVASGQEDRVLAGGIEMMSRVPFLSDRADFYTDTALVPRRRFIPPVLAADRLAHVRG
                     ITRAQLDACALVSQQRAEGSDADPVLQASRLAAGKLTGEECIRRGTTAERLAALPGAF
                     GALQADYAEALEGETFEALHSLSHAPPICDGAGLALLGTRGLGPSPRARVLAYAESGG
                     DPAASLTAGFAAMDKALARAGLGLGAMDRIEFM"
BASE COUNT          487 a         1025 c         1082 g          548 t
ORIGIN      
        1 cccctgatca acatggcctc gatcggcggg atcacgatga gctggctcaa ggtggtgggc
       61 accatggtgc tgcaccaccc gttcgatcct caggtttact tgcgccagat cgcgaccgag
      121 cggcccggct ttaccatcgc cccgcccgcg gtgctcaaca tgctgctgca gaacgaggca
      181 ttgctcgcct cggtcgatct ttccagcctg cattcgatcg gctccggctc agccccgctc
      241 gcccctgcca tggtcaaggg cttccaggat cggttcgcca tcatggtgat caacctgttc
      301 ggctcgaacg aggggatgtc gctggtgacc ggccccgacg acgtgcccga tcctgaagag
      361 cgcgccagct atttcccgcg ccgcccgttg ttcgagccgc cctgcggtgg cgagccggcc
      421 cgccatctcg aaacccggct ggtcgcgccc ggctgcggcg gggagattac cgagccaggg
      481 gtgccgggcg agctgcaaat ccgcgggccc accgtgttcg agggctattg gcgcgccagc
      541 gagcagaccg ctgcggcctt taccgaagac ggcttcttca agaccggcga tctgttccag
      601 atcgagggcg agggacggtt ttacctgttt gtcggacggt gcaaggactt gatcattcgc
      661 ggcggcgtga acattgcgcc cgaggagatc gaccagctcc tgggcgggca tcccgatctg
      721 gccgaggcct gcgtcttttc gctgcccgat ccggttctgg gcgaacgggt cggcctggct
      781 gccgtcccgc gcgatgggcg cgcgctcacg ctcgaggatg tgaccggatt cctgcgccgg
      841 catgatctgg cggtgttcaa attgcccgag cgactgttcg tgttcgacag cctgccgcgc
      901 aatgtgacaa acaaggtgat gcgcggcgaa gtccgcgaga tggcgctcaa gttgatggaa
      961 caaggaggtt gaccatggcc gcagaggtgt tcgtgctggg cggggcccag agcgattttg
     1021 cccgcaacct cgatcgcgag ggcggcggaa tgttcgaact gttccgcgat gtggccgaag
     1081 cggccttcgc cgcgacgggg atcgaaccgg cagaggtcga ggtggcccat gtcggtaatt
     1141 tcgtaggcga gctgtttgcc gggcaaggcc agcttggcgg gttcttcgga cacgtccatc
     1201 ccgatcttgc cggtgttccc gccgcgcgcc acgaagctgc ctgcgcctcg ggatcgatcg
     1261 cgctgctcgc cgccggagcg gagatcgagg cgggccgcta tggcctggcg ctggtgctgg
     1321 ggatcgaact gatgcgcaat gttcccggcc agcgcgccgc cgaatacctg ggctctgccg
     1381 cctgggcagg gcgcgaggcg caggaggcgc gctatttgtg gccctacatg ttctcgcagg
     1441 tagcccagga gtattcggaa cgcttcgggc tcgatcgggc gcacctcaac gcgatcagcc
     1501 agatcgcctt tgccaatgcc aagcgcaatc ccaattcgca gacccgcggc tgggcgatca
     1561 cgcccgaaca cctgagcgat gccaatgacg agctcaatcc gccgatcgaa ggatcgctgc
     1621 gcaaggccga ttgcggccag gtaaccgatg gtgcggcagc ggtgttcctc gcctcgcgcg
     1681 cggtggccga gcgctatgcc gcacggcgcg ggatcgcgct ggaaagcatt ccccggatca
     1741 agggctgggg ccacacaacc gccccgttgc tttattcgac caaggtatcg gccagccgcg
     1801 gtcagcccta tgtcttcccc tctgtccgca aggcgatgat ggatgcgctg ggccgggccg
     1861 ggatggccga tgtctacgcc tgcgacgggg tagaggtgca cgattgcttc tcgatcaccg
     1921 aatatatggc gatcgaccac ttcgggatta ccgcgccggg tgaaagctgg aaggcggtgg
     1981 aggacggcac catagcgctc ggcgggcgcc tgccggtcaa cccttcgggc ggactgatcg
     2041 ggcttggcca tccggttggc gccaccggcg tcaggatgat gctcgatgcc tggcgccagg
     2101 tcaccggcaa tgccggggac tatcaggtcg aaggggccag gaacttcgcc acgttcaacg
     2161 ttggcggcag tgcgaccact tcggtcagct tcgtggtggg ggtctgacac cggcgtggaa
     2221 gaggtttatc tctacgatgc cctgcgcaca ccgcgcggca aggcgcgcgc cgatgggggc
     2281 ctggccggac tgactccgcc cgaactggtc ggcgtgctga tcgatggcct gcgcgaacgg
     2341 accggcgatg cctgtgccaa cccgggcgca ttgctgctgg gctgcgtcac gcaaagcggc
     2401 gcccaaggcg ggcacgtcgc catggcggcc aagcaataca gcggcctgcc cgatgattgc
     2461 gccgcgcaaa cgctcaacaa ctattgctcc tccgggctta gtgcgatcgg ccacggcgtt
     2521 gccatggtcg ccagcggaca ggaagaccgc gtcctggccg gcgggatcga gatgatgagc
     2581 cgcgtgccct tcctttcgga ccgggccgat ttctataccg acaccgccct cgtcccgcgc
     2641 cgccggttca tccccccggt gctggcggcc gaccggctgg cccacgtccg gggtatcacc
     2701 cgggcgcaac ttgatgcctg tgcattggtt tcgcagcagc gcgccgaggg cagcgatgcc
     2761 gatccggtgc tgcaggcttc gcggctggcc gccggcaagc tgaccggtga ggagtgcatc
     2821 cgccgcggca ccaccgccga gcgcctggcc gcacttcctg gtgccttcgg ggcactgcag
     2881 gccgactatg ccgaggcgct ggagggtgag acatttgagg cgctgcatag cctcagccac
     2941 gccccgccga tctgcgacgg ggccgggctg gcactgctag gcacaagggg ccttggccct
     3001 tcgccgcggg cccgggtgct ggcctatgcc gaaagcggcg gcgatcccgc cgcctcgctc
     3061 accgcaggct ttgccgcgat ggacaaggcg ctggcccgtg ccgggctggg cctgggcgcg
     3121 atggaccgga tcgaattcat gg
//
LOCUS       DASUDV010000096         5702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1659690,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000096 DASUDV010000000
VERSION     DASUDV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659690"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFV30_02055"
     CDS             <1..346
                     /locus_tag="VFV30_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4846902.1"
                     /translation="LPGAPDYKFSAGVQYAIPAGEMTFTPRADFIYTGKSVGNIFGGT
                     VNEVPSFTQVNAQLQLDGPDKKWFARLWVQNLFDKDSITGLYVTDQSSGNYTNVFTLE
                     PRRYGISAGIKF"
     gene            420..2156
                     /locus_tag="VFV30_02060"
     CDS             420..2156
                     /locus_tag="VFV30_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4846903.1"
                     /translation="MIDVTRRSALGLLAAAPLAGTGGAGSTAFQSVTVFEARRFITME
                     PGRPACRFVAVSDGLVLALGDSLEELEPWTRGRALRVDRSMAGRIVMPGLIDPHLHPM
                     QASIMLNLPFLAPDDWNLPRGRSPGVVGQAAYRQRLAAMLAASKDDPFITWGYHELFH
                     GPLSRADLDALAPGRAVLVWQRSFHDIIVSTALLERWGVRSAADFAALLASVRADPAH
                     SDYARGYFSETGLLAALAKLRPVLLGPGKIAAGMAALQTVMRQNGVTTASDMSTGVFA
                     DFETEAGLIKAAFDRPASAARMMLMPIATQLDAVEDLPAWLDSAAKRFAAPNVRLDRR
                     VKMLADGAFFALNMRMNPPGYTDGHTGRWITEPAVLVGQFRRFWQAGFSLHIHVNGDE
                     GLDVVLSALADLAPSRGQTITLEHLGYSTEAQNRRIARMGLMVSAQPNYIRVLGDAYG
                     REGLGPGRAEQINRLGSLERKGVPLGLHSDFNMAPIDPLYLAWIAANRITIEGRTKAP
                     DERLSLDKALRAITIEAAQVIGMDAMVGSLAPGKRADMAVLDRDPYAGGAAGLRDVRV
                     LGVVFEGRPAAA"
     gene            complement(2343..2954)
                     /locus_tag="VFV30_02065"
     CDS             complement(2343..2954)
                     /locus_tag="VFV30_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4846904.1"
                     /translation="MQKRYSLALVDGNSRRRAAITHALAGSGIHVEPFEDLGEITFRW
                     PRTGAILVADQGPVVEDLINHMSLHGEWLPVIAFSEDPGTERIVRAVLAGALDYLEWP
                     CDPAKIVTAVTLAESASASIGSLRLREARARSRVQKLTRREREVLEGVADGLSNRLIG
                     EKLQISPRTVEIHRANMLNKMGASHTSEAIRIAIEASLVGQAA"
     gene            complement(3101..3502)
                     /locus_tag="VFV30_02070"
     CDS             complement(3101..3502)
                     /locus_tag="VFV30_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HEX4846905.1"
                     /translation="MQILRSPQDVYRKVDLDARIAGADPQQLVLLCYEQLGTALGSAV
                     HAAQAGDNARKSAALTRALSAITALQMGLDTAAPVSAALATFLDAARHTVLGTVIAFD
                     APALERLKSDVTELAAAFRQAGSAADKVRNG"
     gene            complement(3504..4925)
                     /gene="fliD"
                     /locus_tag="VFV30_02075"
     CDS             complement(3504..4925)
                     /gene="fliD"
                     /locus_tag="VFV30_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX4846906.1"
                     /translation="MATTAATSSIVTTLGAGSGIDMAALAQGLANASFAAKIDRNTAR
                     SETVERQISAASALKSALLQLSSSIGDRVRTGDLSSQPLIANPAVAAVSRGTLAGRGS
                     YGLEVTQLAANQLLASAPFAAATTPTGSGSLTFRFGTIAAGAFTADAARSPATVTIPS
                     GATLADAASAINAAGIGITAYVVTGTAGAHLMFKGQDGAANGFVIEAAETPGEEGLAA
                     LAWTPASAPTRLHGAARDAEFKLDGLAMTRPSNTLPDLVPGLSLTLTGTNPGAPTTIR
                     FSDPGAAVQTFMGDLVSALNELAGELKTQTDPASGDLARDSGARSLRRSLAQLAGSAI
                     MPAALPGDPATLADLGLATNRDGTFRLDTARLTATLKASPEGVAAMFTTGIHGVYASF
                     DRLSRRLTAASDPGTLAGSLSRLTAQKRDLGEEAAKLAEAQEKLRSRLTQQFAGVDSR
                     VAASKSTLSFLQNQIDAWNAQKG"
     gene            complement(4956..>5702)
                     /locus_tag="VFV30_02080"
     CDS             complement(4956..>5702)
                     /locus_tag="VFV30_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX4846907.1"
                     /translation="GLKRMFGAQGWIEVGKGVLKVLLLGAICYAWARGHLAGLLGLGQ
                     AASLGGALAQAWAALIGLLFWLSGGLVLIALIDWPVQWLRRFMRLRMSHQEMRDEHKE
                     SDGSPERKAAQRQRQRQYASGAVAKAMREAQFVLTNPSHFSVAMGYDPLKAGAPVVLA
                     KGRGEKALAMRELARELDVPLIAAPALARSVYFTTREQQMIREELYAAVAAVLAYVFS
                     LRRGEPRELPPIEIPAALRFDAEGRIETAA"
BASE COUNT          807 a         1942 c         2053 g          900 t
ORIGIN      
        1 gctgcccggc gccccggact acaagttctc ggccggcgtg cagtacgcga tcccggctgg
       61 tgagatgacc ttcaccccgc gtgcggactt catctacacc ggcaagtcgg tcggcaacat
      121 cttcggcggt accgtcaacg aggtgccgag cttcacccag gtgaacgctc agctccagct
      181 cgacgggccc gacaagaagt ggtttgcccg cctgtgggtg cagaacctgt tcgacaagga
      241 ctcgatcacc ggtctctatg tgaccgacca gtcgtcgggc aactacacca acgtgttcac
      301 cctcgagccg cgccgctacg gcatctcggc ggggatcaag ttctaagcac tcctgggagg
      361 gatgcttatg cgggggctcc ggggaaaccc ggggcccctt tgccataagg gggaatgcca
      421 tgatcgacgt gacccggcgg tctgcgcttg gcctgctggc cgccgctccg cttgccggca
      481 cgggcggcgc cggatcgacc gcctttcaat ccgtcaccgt cttcgaagcg cgacggttca
      541 tcaccatgga gccgggccgg cccgcctgcc ggttcgtcgc cgtctctgac gggctggtgc
      601 tggcgctggg ggacagcctg gaagagctgg aaccctggac ccgcggccgg gcgctgcggg
      661 ttgaccgcag catggccggc cggatcgtga tgccgggcct gatcgatccg catctccatc
      721 cgatgcaggc ctcgatcatg ctcaatctgc cgttcctggc cccggacgac tggaacctgc
      781 cgcgcgggcg gtcgccgggt gtggtcgggc aggccgccta tcgccagcgg ctggccgcga
      841 tgctggcggc gtccaaggac gatcccttca tcacctgggg ttaccacgag ctgttccacg
      901 gccccctctc gcgggccgat ctcgatgcgc tggcgccggg ccgggcggtg ctggtctggc
      961 agcgcagctt tcacgacatc atcgtctcca ccgcgctgct ggagcgctgg ggcgtgcgca
     1021 gcgcggcgga ctttgccgcg ctgctggcct cggttcgcgc cgatccggcg cacagcgact
     1081 atgccagggg ctacttcagc gaaaccggcc tgttggcggc gctggcgaag ctgcggccgg
     1141 tcctccttgg ccccggcaag atcgcggcgg ggatggcggc cctgcaaacc gtcatgcggc
     1201 aaaacggggt caccaccgcc tcggacatga gcaccggggt cttcgccgat ttcgagaccg
     1261 aggccggcct gatcaaggcg gcctttgacc ggccggcgag cgcggcgcgg atgatgctta
     1321 tgccgatcgc gacgcagctg gatgcggtgg aggaccttcc ggcctggctg gacagcgcgg
     1381 ccaagcgctt tgccgcgccg aacgtccggc ttgaccggcg ggtcaagatg ctggccgacg
     1441 gggccttctt cgcgctcaac atgcggatga acccgcccgg ctacaccgat gggcacaccg
     1501 gccgctggat aaccgagccg gccgtgctgg tcggccagtt ccgccgtttc tggcaggcgg
     1561 gcttctcgct ccacatccac gtcaacggcg atgaagggct cgatgtggtc ctctccgcgc
     1621 tggccgacct ggcgccgtcg cgcggccaga cgatcacgct ggagcatctc ggctattcga
     1681 ccgaggccca gaaccggcgg atcgcgcgga tgggcctgat ggtgtccgcc cagcccaact
     1741 acatccgggt gctgggtgat gcctatggcc gcgaggggct ggggccggga cgggccgagc
     1801 agatcaaccg gctcggctcg ctggagcgaa agggcgtgcc gctgggcctg cacagcgatt
     1861 tcaacatggc accgatcgat ccgctgtacc tcgcctggat tgccgccaac cggataacga
     1921 tcgaggggcg gaccaaggcc ccggacgaac ggctcagcct cgacaaggcg ctgcgggcga
     1981 tcacgatcga ggccgcgcag gtgatcggga tggacgcgat ggtcggctcg ctggcgccgg
     2041 gcaagcgcgc cgacatggcg gtgctggacc gggatcccta tgccggcgga gcggcggggc
     2101 tgcgcgatgt ccgggtgctg ggggtggtct tcgaaggacg gcccgccgcg gcctgaagct
     2161 ctgcggaatg cgccggtccc cggggtgctg ccccggccat cggggaacgg gaggccgggg
     2221 cagcggggcg ggggaagagc ggggtttcgt acttccttcc agggacttgg caccgtctgg
     2281 tgcacccccc tgatagccac gccagcgtta gccgggcgtg tgcccgccgt taggtagttc
     2341 gcctaggccg cctggccgac cagcgaggct tcgatcgcga tccggatcgc ttcgctcgtg
     2401 tggctggcgc ccatcttgtt gagcatgttg gcgcggtgga tttccacggt gcgcgggctg
     2461 atctgcagct tctcgccgat cagccggttc gacaggccgt cggccacgcc ctccagcacc
     2521 tcgcgctcgc ggcgggtcag cttctgcacc cggctgcggg cccgggcctc gcgcaggcgc
     2581 aggctgccga tgctggccga ggcgctttcg gccagggtca ccgcggtgac gatcttggcc
     2641 ggatcgcagg gccactccag atagtcgagc gcccccgcca gaacggcgcg gacgatccgc
     2701 tcggtgcccg gatcctcgct gaaggcgatc accggcagcc actcgccgtg cagcgacatg
     2761 tggttgatca ggtcttccac caccggcccc tgatcggcga ccaggatcgc cccggtgcgg
     2821 ggccagcgga aggtgatttc gcccaggtcc tcgaacggtt ccacgtggat gccgctgccc
     2881 gccagagcgt gcgtgatggc cgcgcggcgg cggctgtttc catcgaccaa agccagactg
     2941 tagcgtttct gcatcggaat gccccctagt ggttagcgcc taggggatta ggccgaagcg
     3001 ctgccgccgc cgcgcccagg tggctccgga ttcgagggac aggatccgcg caaacccgta
     3061 ggttacggcg ccttcccgcg cggaattccg ggccggggat tcaaccattc cggaccttat
     3121 cggccgcact gccggcctgg cgaaaggcgg cggcgagctc tgtcacatcg cttttcagcc
     3181 gttccagcgc cggggcatcg aaggcgatga ccgtgcccag cacggtgtgc cgggcggcat
     3241 cgagaaacgt tgccagggcg gcgctgaccg gcgcggcggt atccagcccc atctgcagag
     3301 ccgtgatcgc cgaaagcgcg cgggtgaggg ccgcgctctt gcgggcgttg tcgccggcct
     3361 gggccgcgtg gaccgccgag ccgagcgcgg tgccaagctg ttcgtagcac agcagcacca
     3421 gctgctgcgg atcggcgccg gcaatccgcg cgtcgagatc gaccttgcgg tagacgtcct
     3481 ggggggagcg gaggatctgc atgtcagccc ttctgtgcgt tccacgcgtc gatctggttc
     3541 tgcaggaacg agagtgtcga tttcgacgcg gccacccggc tgtcgacccc ggcgaactgc
     3601 tgggtcaggc gggagcggag cttctcctgg gcctcggcca gcttggccgc ttcctcgccc
     3661 agatcgcgtt tctgcgcggt cagccggctc agcgatccgg ccagggtgcc gggatcgctc
     3721 gccgcggtca ggcggcggct gagccggtcg aagctggcgt agacgccgtg gatcccggtg
     3781 gtgaacatcg ccgccacccc ctcgggcgag gccttgagcg tggcggtgag ccgcgcggta
     3841 tcaagccgga aggtcccgtc gcggttggtc gcgaggccca ggtccgcaag ggttgccgga
     3901 tcgcccggca aggcggcggg catgatcgcg ctgccggcca gctgggccag gctgcggcgc
     3961 aggctgcgcg ccccgctgtc gcgggccaga tcgcccgagg ccggatcggt ctgggtcttg
     4021 agctcgcccg ccagttcgtt gagcgcggag accagatcgc ccatgaaggt ctgcaccgcc
     4081 gcgcccggat cggaaaagcg gatcgtggtg ggcgcgccgg ggttggtgcc ggtcagcgtc
     4141 aggctgaggc ccgggaccag atcgggcagg gtgttggacg gccgggtcat ggccagccca
     4201 tcaagcttga attcggcatc gcgggccgca ccgtgaagcc gggtgggggc gctcgccggg
     4261 gtccaggcca gtgcggccag cccttcctca ccgggtgttt cggcggcttc gatgacaaag
     4321 ccgttggccg cgccgtcctg ccccttgaac atcaggtgcg ccccggcggt accggtcacc
     4381 acataggcgg tgatcccgat cccggcggcg ttgatcgcgc tggcggcgtc ggccagcgtc
     4441 gcgccgctgg ggatggtgac cgtggccggg ctgcgggccg cgtcggcggt gaaggccccg
     4501 gcggcgatcg tgccgaagcg gaaggtcagg ctgcccgatc cggttggcgt ggtggcggcg
     4561 gcaaagggcg cgctggccag caactggttg gcggcaagct gggtgacttc caggccatag
     4621 ctgccgcgcc cggccagggt cccgcgcgag acggcggcca ccgccgggtt ggcgatcaac
     4681 ggctggctgg agaggtcccc ggtccggacc cggtcgccga tcgagctcga cagctgaagc
     4741 agcgcgctct tgagcgcgga ggccgcggaa atctgccgct cgaccgtttc gctgcgggcg
     4801 gtgttgcggt caatcttggc ggcgaaggag gcgttggcga ggccttgcgc cagcgcggcc
     4861 atgtcgatcc cgctgcccgc gcccagcgtg gtcacgatcg aggatgttgc cgcggtggtt
     4921 gccatgcagc ctagaacggc agcgcgcggc cgggcttaag cggctgtttc gatccgcccc
     4981 tcggcatcaa agcgcagcgc ggcggggatc tcgatcggcg gcagctcgcg cggctcgccc
     5041 cggcgcagcg agaagacata ggccagcacc gcggcgaccg cggcatagag ctcctcgcgg
     5101 atcatctgct gctcgcgggt ggtgaaatag accgagcggg ccagcgccgg cgcggcgatc
     5161 aggggcacgt ccagctcgcg cgccagctcg cgcatcgcca gcgccttttc gccgcggccc
     5221 ttggccagca ccaccggggc gccggccttc agcgggtcat agcccatcgc gaccgagaaa
     5281 tggctggggt tggtcagcac gaactgcgcc tcgcgcatcg ccttggccac ggcgccgctg
     5341 gcgtactgcc gctgccgttg gcgctgggcg gccttgcgct cgggcgaacc gtcgctttcc
     5401 ttgtgctcgt cgcgcatttc ctggtggctc atccgcagcc gcatgaagcg ccgcagccat
     5461 tgcaccggcc agtcgatcag ggcgatcagc accagccctc cggacagcca gaacagcagc
     5521 ccgatcagcg cggcccaggc ctgggccagc gccccaccca gcgaggcggc ctggcccagg
     5581 ccgaggaggc cggccagatg gccgcgcgcc caagcatagc aaatcgcccc cagcagcagc
     5641 accttgagca cgcccttgcc cacttcgatc cagccctgtg cgccgaacat ccgcttgagc
     5701 cc
//
LOCUS       DASUDV010000097         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_157667,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000097 DASUDV010000000
VERSION     DASUDV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157667"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFV30_02085"
     CDS             complement(<1..457)
                     /locus_tag="VFV30_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX4846908.1"
                     /translation="MDSRATGFAGLEADELARTRAGEDSNLRLPVELAVTPPFTIGEL
                     QVEPATRRVLYLDGESETLEPRVMETFVALYRAGGAVMSRDDLLAACWRGTVVGEDAI
                     QRVIQRLRKVAGRSHAFSIETITKAGYRLIPTAAETEGVRGDAPSLAVLP"
     gene            complement(754..1470)
                     /locus_tag="VFV30_02090"
     CDS             complement(754..1470)
                     /locus_tag="VFV30_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4846909.1"
                     /translation="MSNSLQSSSDPEVLRTVLAPGLLAGFPAEARAELIAAGRTRRVA
                     AGQLIQQRGDPVGTFWLVESGHVRIGQFDPDGAFNAIAVLGPGDSFGELALLAGRRRV
                     VDAVAVGPTRLRAIPAAALHRLARTNPAVASALIEALARQFHEALDLLAAFRRSNGAA
                     RLVRTLAVLVQDRPGPVRLALGQQALADLVGTSRMTVSTALASLERQGLIRRGYGWIE
                     VDRPEALRALTGTAADPGPR"
     gene            1526..>2003
                     /locus_tag="VFV30_02095"
     CDS             1526..>2003
                     /locus_tag="VFV30_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX4846910.1"
                     /translation="MPTENWIIIAIFVGFALIELVRSNLLHKPEQTRDDWIVEVVSTV
                     ALLVITQPLILFTVDRALGAWQPEWRGALAGLNVFAAVALFLVFDDMAQYWWHRASHS
                     TPWLYNLHRAHHNARYMSVRLVYRNNLFYYTMMPGIWCSAVLVYLGLGWVYAGYIVI"
BASE COUNT          339 a          691 c          645 g          328 t
ORIGIN      
        1 agggcagcac ggccaggctg ggggcatcgc cgcgcacgcc ttcggtctcg gccgccgtgg
       61 ggatcagccg gtaacccgcc ttggtgatcg tctcgatcga aaaagcatgg gaccggccgg
      121 ccactttgcg cagccgctgg atcacgcgct ggatcgcatc ttcgccgacc acagtgccgc
      181 gccagcaggc ggcgagcaga tcgtcgcggc tcatcaccgc gccgccggcc cggtagagcg
      241 caacaaaggt ttccatcacc cgcggctcca gcgtttcgct ttcgccgtcg aggtacagga
      301 cccgccgggt ggccggctcc acctgcagct caccaatggt gaagggcggg gtgacggcca
      361 gctcgaccgg caggcgcagg ttcgaatcct cgcccgcacg cgtccgggca agttcatcgg
      421 cttccagtcc tgcaaacccg gttgcgcgtg aatccacgcc ggcagctacc tttgtctgtt
      481 gccagcgaac tatcgccgca actgccgcaa aagcaacgaa attcggcggt ttttgaggtt
      541 ccctttcggc cgtgccagcg gccgtagggc aacaatcatc gggaaatcat cgggttgcgg
      601 tcctgtccct tgccggcggg gtggggcaat aggctctcaa ctttcctccg gtgagagcag
      661 gtggaaaggt tgctgttcgg aatgcgaccc gacggatggc aacaaactca acttcgccgc
      721 ggtattgccc caagcagaac cgcggcgaag cttttatcgg ggccccggat ccgccgcagt
      781 gccggtcagt gcgcgcaacg cttctgggcg gtccacttca atccagccat agccgcgccg
      841 gatcaggccc tgccgttcca gagacgcgag ggcggtgctg accgtcatcc gcgacgtccc
      901 caccagatcg gcaagggcct gctggcccag cgccaaccgc accggaccgg gccggtcctg
      961 caccagcacc gccagggtcc ggaccagccg cgcggcgccg ttggagcggc gaaaggcggc
     1021 cagcaggtcc agcgcctcgt ggaattgccg ggccagcgct tcgatcagcg cgctggcgac
     1081 cgccggattg gtcctggcca gccggtgcag tgcggcggcc gggatcgcgc ggagccgggt
     1141 cggaccgacg gccaccgcat cgaccacccg ccgtcgcccg gccagcagcg ccagctcacc
     1201 aaagctgtcg cccggtccca gcaccgcgat cgcgttgaac gccccgtcgg gatcaaactg
     1261 gccgatgcgg acatggccgc tttcgaccag ccagaaggtg ccgaccggat cgccccgctg
     1321 ctggatcagc tggcctgcgg caacgcggcg cgtacggccc gccgcgatca gttcggcccg
     1381 ggcctcggcc ggaaagccag ccagcaggcc cggtgccagc actgtccgca gcacctccgg
     1441 atcggacgaa gattgtaagg aattggacat tcgtagcagc cggatggcgc tataccgtcc
     1501 gaaagacaag gggggagagt ctggcatgcc gaccgaaaac tggatcatca tcgcgatctt
     1561 cgtgggcttt gcgctgatcg aactggtgcg ctcgaacctg ctgcacaagc ccgagcagac
     1621 ccgcgatgac tggatcgtcg aagtggtcag cacggtcgcc ttgctggtca tcacccagcc
     1681 gctgatcctg ttcacggtcg accgggcgct gggcgcctgg cagcccgagt ggcgcggcgc
     1741 gctggccggg ctcaacgtgt tcgccgctgt cgctctgttc ctggtgttcg acgacatggc
     1801 ccaatactgg tggcaccgcg cctcgcactc cacgccctgg ctctacaacc tccaccgcgc
     1861 ccaccacaac gcccgctaca tgagcgtccg gctcgtttac cggaacaacc tgttctatta
     1921 tacgatgatg ccagggatct ggtgctcggc agtgctggtc tacctcgggc tcggctgggt
     1981 ctatgccggc tatatcgtga tca
//
LOCUS       DASUDV010000098         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1924933,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000098 DASUDV010000000
VERSION     DASUDV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924933"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFV30_02100"
     CDS             complement(<1..270)
                     /locus_tag="VFV30_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX4846911.1"
                     /translation="MKKLKKKKFAKLYAPLEEELVAMARWAAATGARVLVLFEGRDTA
                     GKGGAIQAVSAPLNPRQCRTVALPKPSEREKTQWYFQRYAAHLPAA"
     gene            375..1298
                     /gene="phhA"
                     /locus_tag="VFV30_02105"
     CDS             375..1298
                     /gene="phhA"
                     /locus_tag="VFV30_02105"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX4846912.1"
                     /translation="MSLPDFTILPEPDPATFTAPLQRPPQVGEDWLEPAQHAYGAQEN
                     AIWDDLFARQMQVLPGRAASVFMAGLDKLDLGRGGVPEFGRLSEELGALTGWSVVPVP
                     MLIPDHVFFWHLANRRFPAGNFIRTRETFDYIQEPDVFHDVFGHVPMLTDPVYADYMQ
                     EYGRAGWKAMRYNRLKALGALYWYTVEFGLILEGADVRAYGAGILSGPTETVFSVEAQ
                     SPNRIMLNVDRVMRTDYVISDLQPTYFVIESFKELYHDTVERDFDRLYRSLSPGFTYA
                     NTAVIDVDDVLHRGTQEYLLRGGRGSGAKPV"
     gene            complement(1351..2160)
                     /locus_tag="VFV30_02110"
     CDS             complement(1351..2160)
                     /locus_tag="VFV30_02110"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX4846913.1"
                     /translation="MDLIVTAILLGIVEGLTEFVPVSSTGHLVLASEIFGYDAATWAV
                     FNVVIQLGAILAVVVLYWRTFWAVGMGLLRLDPMSLAFVRNVLLAFLPSAVLGFLLKD
                     QIRAMLGSPMIVGWALILGGIAILAIEKWAKPGPASGVAQLPARQALGVGLFQCISMI
                     PGVSRSGATIMGALAMGIERRTAAEFSFFLAIPTMMGATTLELWDSRAELMAGTGPVG
                     WTEIAVGFVVSFVVALAVIKAFVAFVSRSGFAPFAWYRIVAGALAVWLLSR"
     gene            complement(2160..>2501)
                     /locus_tag="VFV30_02115"
     CDS             complement(2160..>2501)
                     /locus_tag="VFV30_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4846914.1"
                     /translation="ARLSGGQRQRIAIARAVLREAPILLLDEATSALDAESERLVQDA
                     LDRLMANRTTIVIAHRLATVRKADRIVVMDHGRIVETGTHDALTRTGGLYAKLASLQF
                     DEAAFAAAGSK"
BASE COUNT          454 a          851 c          787 g          409 t
ORIGIN      
        1 ggctgccggc agatgcgcgg catagcgctg gaaataccac tgggtctttt cccgctcgct
       61 cggcttgggc agggcaacgg tgcggcactg gcgcgggttg agcggagccg aaaccgcctg
      121 gatcgccccg cccttgcccg cggtgtcacg cccttcgaac agcaccagca cccgcgcgcc
      181 ggtggccgcg gcccagcgcg ccatggcaac cagttcctcc tccagcggcg catagagctt
      241 ggcgaacttc ttcttcttga gctttttcat ggtctgcgat tccttgctgg cgcgatggtg
      301 cgcccggcat ccgcgccaat caagcacgct tgacgctctt gctgtcccaa aaggaaatgg
      361 ttacagatgc aaccatgtcc ctgcccgact ttaccatcct gcccgaaccc gatccggcca
      421 ctttcaccgc gccgctgcag cgcccgccgc aggtcggcga agactggctc gagcccgccc
      481 agcatgccta tggggcgcag gagaacgcga tctgggacga tctgttcgcc cgccagatgc
      541 aggtcttgcc cggccgcgcc gccagcgtgt tcatggccgg gctcgacaag cttgacctgg
      601 gccggggcgg ggttcccgaa ttcggccgtc tgtcggaaga actcggcgcg ctcaccggct
      661 ggtcggtggt gccggtgccg atgttgatcc ccgatcacgt gttcttctgg catctcgcca
      721 atcgccgctt cccggccggc aatttcatcc gcacgcggga gaccttcgac tacattcagg
      781 aacccgatgt attccacgat gtcttcggcc atgtgccgat gctgaccgat ccggtctatg
      841 ccgattacat gcaggaatat ggccgcgccg ggtggaaggc gatgcgctac aaccggctga
      901 aggcgctggg cgcactctac tggtacacgg tcgaattcgg cctgatcctg gagggcgcag
      961 atgtccgggc ctatggcgcg gggatccttt cggggcccac cgaaacggtc ttttcggtcg
     1021 aggcgcaatc gcccaaccgg atcatgctca acgtcgatcg ggttatgcgc accgactatg
     1081 tgatcagcga cctgcagccg acctacttcg tgatcgaaag cttcaaggag ctgtaccacg
     1141 acacggtcga gcgcgatttc gaccggctct accgctcgct cagcccgggc ttcacctatg
     1201 ccaacaccgc ggtgatcgac gtggacgacg tgctgcaccg cggaacccag gaatacctgc
     1261 tgcgcggcgg gcgcggcagc ggagcaaagc cggtctgaca gggtgacaga ggccggtgac
     1321 gcaggaaagg ttgttcgcag tcctggccgc tcagcgcgac agcagccaca ccgccagtgc
     1381 cccggccacg atccggtacc aggcaaacgg cgcaaagccc gagcggctga cgaaggcgac
     1441 gaaggccttg atcaccgcca gtgcgaccac gaagctgacc acgaagccca ccgcgatctc
     1501 ggtccagccg accgggccgg tcccggccat cagttcggcg cggctatccc acaattcgag
     1561 cgtggtggcg cccatcatgg tcgggatcgc caggaaaaag ctgaattcgg ccgcggtgcg
     1621 ccgctcgatc cccatcgcca gcgcgcccat gattgttgcc cccgaacgcg aaacacccgg
     1681 aatcatcgaa atgcactgga acagccccac ccccagcgcc tggcgcgcgg gcagctgggc
     1741 aacaccgctg gccgggccag gcttggccca cttttcgatc gccaggatcg cgatcccgcc
     1801 caggatcagc gcccagccga cgatcatcgg actgcccagc atcgcccgga tctggtcctt
     1861 gagcagaaaa ccaagcaccg ccgagggcaa gaaggcgagc agaacgttgc gcacgaaggc
     1921 caggctcatc ggatcgagcc tgagcagccc catgccgacc gcccagaacg tgcgccagta
     1981 gagcaccacc acggcaagga tcgcgccgag ctggatcacc acgttgaaga ccgcccaggt
     2041 ggcagcatcg tagccgaaga tctccgaggc gaggaccagg tgtccggtcg atgaaaccgg
     2101 cacgaattcg gtcagccctt cgacaatgcc caggaggatt gcggtaacga tcaggtccat
     2161 catttgctcc cggcggcggc aaaggccgct tcgtcgaact gcaggctggc gagcttggca
     2221 tagagcccgc cggtgcgggt cagggcatcg tgagttccgg tctcgacgat ccggccatga
     2281 tccatcacca cgatccggtc ggccttgcgc accgtagcca ggcggtgggc gatcacgatc
     2341 gtcgtgcggt tggccatcag ccggtcgagc gcgtcctgga ccagccgttc gctttcggca
     2401 tcgagcgcgc tggtggcctc gtcgagcagc aggatcggcg cttcgcgcag caccgcgcgg
     2461 gcaatcgcga tgcgctggcg ctgcccgccc gaaagccgtg c
//
LOCUS       DASUDV010000099        12284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1924967,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000099 DASUDV010000000
VERSION     DASUDV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924967"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VFV30_02120"
     CDS             complement(<1..1368)
                     /locus_tag="VFV30_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4846915.1"
                     /translation="MIEGERNPIAETGEDGLLADDFEQLEQAINRALAADSSLSESTA
                     AKPLRPVAIFAEAWQVCGHDAAAGTLAEDWPAAFTDSERAELECELPDPGPRPLIRFL
                     HPAEASPVMVLLLPVRPYAHWPMVARWLKGRGAAAAPSHAALRLAALDPRQDRFNRAR
                     SMFRLTPSEERLVCRLAQAGTLRAAAEIEGVTYETARSALKQALAKSGHPRQPALVGA
                     ALKLGALDEPLELDSEGPLREIFGLSPRQASIARLFALGQSREELARQLGHSVETVKA
                     ELKVIYTALGVGNATALAVIAAQVGTAARMLAAQSVGGVDLAASSEPVRLLPRPTRGG
                     RIAFADYGPADRIPTLHLHTATTSRYLPRSYVTALQAAGLRPVAVDRPGFGMTEIVEG
                     NYFEQTALDLIDVADALGAERFNLIARGASPVGWLVRHFRDRLERVVVTNPEPEPKSD
                     HSMGGI"
     gene            complement(1463..2095)
                     /locus_tag="VFV30_02125"
     CDS             complement(1463..2095)
                     /locus_tag="VFV30_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4846916.1"
                     /translation="MPTFYDCTPAPSPRRARIVLAEKGIPHAVKIVDLSKGEQLTPEF
                     RAINPACTVPALVLDDGAVLTDNAGIAAWADAAFPEAPLLGSTPLEKAAVASWNSKIE
                     GECFMAIAEVLRNTAKGMKDRALPGPHNYAQIPELAERGRARLGHFLDRFEAHMTGRE
                     WVAIDRFSLADITAGVALDFAGWVKVDVNAGRPAIAAWRARLAERPSFQL"
     gene            2271..2969
                     /locus_tag="VFV30_02130"
     CDS             2271..2969
                     /locus_tag="VFV30_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4846917.1"
                     /translation="MEGDDAMAYADTNTTTRKLGTGAAVLLIEAGVAAALVTGLAMTG
                     TIKPDTIIDTFDIPKPQVTPTPPPPDKPQVRRNESTIDRPDVLIKLPPVTDTLPEVEL
                     KPSGDNGKDTVGDVTFPQPEVTPDPPQPPRFTPKRAVPRGSTAKWVTTNDYPASELRA
                     EHQGTTRYRLSIDAGGAVTDCTVTASSGYPGLDRAACETVKRRAKFEPATDHTGARAP
                     GSFSGSVTWQIPKE"
     gene            3196..4515
                     /gene="chrA"
                     /locus_tag="VFV30_02135"
     CDS             3196..4515
                     /gene="chrA"
                     /locus_tag="VFV30_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX4846918.1"
                     /translation="MTAAAPSPSFADLVRLSARVGCLSFGGPVGQIALMHREFVEERG
                     WIGEDEYLHALNFCHLLPGPEAQQLATWIGWRLHGVKGGLAAGLLFVVPGALVMLALS
                     VLYAQAAGLPWFSALFLGIKAAVLAIIAQALLRIAGRTLDTAFKRGIAAAAFLALAFF
                     ALPFPLVVLGAGLIGALAAVRRPDLLALKPLAAARPAAPRPWGHTIRAVLIGGALWAA
                     PLLAVLAVLGRGHVLWDIGTFFSQLAVVTFGGAYAVLAYMGQQAVQDFGWLSAREMAD
                     GLGLAETTPGPLIMVTQFVGYLAAFRAPEPFSPVVAGVIGAGLTTWVTFAPCFVWIFA
                     LAPWLERLEHARRLKGALSAITAAVVGVIASLALWFALHVLFTDLIPARLGPAGYDLP
                     VLASLDWRAALLAALSAVLLFRARWSVLKVLPLAALGGLVLGTLTGS"
     gene            4561..5412
                     /locus_tag="VFV30_02140"
     CDS             4561..5412
                     /locus_tag="VFV30_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX4846919.1"
                     /translation="MQLSVFPAETRQAFAAAYPATPAKLHHSLLGHPLLTLDALAALA
                     EALPAHEIEYNPGQLPIGIKPEDVPKSDLTVTETIRNIGKAGSWVALKRIEQVPAYRD
                     LLHGILGQLRDIVEPRTGEMMQLEGFVFITSPGSVTPFHFDPEHNILLQVMGHKTMTL
                     FPVEDEAMFSADIHEKFHLGLHHRNLPWYDGYAEKGTPIHIGPGEAIHVPVKAPHWVQ
                     NGDQPSVSLSITWRSDWSYAEADARAFNHVLRKIGLRPASPRPFPGRNLGKSLAWRAI
                     RRLRGAA"
     gene            complement(5409..7535)
                     /locus_tag="VFV30_02145"
     CDS             complement(5409..7535)
                     /locus_tag="VFV30_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HEX4846920.1"
                     /translation="MRLARIVMVAAAWTGLVGAGAPDTALPDSSPASAPSAAPAGAVF
                     PRPLIAPAPAWVDIAPLPKAPRDADGAATIDLLGDIQVRFGRDGDASYYNAAWVIGTS
                     HGLDSGALKIDWDPALEQLTIHTYRVRRDGKVIDLLGDGSALKVIQRETRMESAMLDG
                     KLTATLQPEDLRVGDVIELAYTVVRRDPAARGFSQYLAGPADGATYGRFRVRLLWDKD
                     KPMQWRAFPGVLQPRLKKTATGSELVADITDTTTPSPPEGAPKRFAVVNAVEITEFKD
                     WQAVSRNFAPLYADAIRLAPDSPVKAEAARIAAITPDPRRRAELALKLVQDQVRYLFL
                     GMNAGGFVPASAEQTWARRFGDCKGKTVLLIALLKELGIEARPVLVNTESGDFVGARL
                     PTMEAFDHVIVEATIDGKSYWLDGTRLGDERLDRLDTPNYRFGLPVTADGAGLVAIVP
                     PALTRPVSSVSLSFDASKGLDLPATVTGEMRFSGDAGSSVRQKFAGYSQADRNRELRK
                     VWRDAFDFISPGKVATRTDPETGDFILTMTGSAQMEWTSDLGTRWYEIDRSRLGWRLD
                     IAREGEIAKDAPYAFDYPDWWSAKVTIVLPYEGRGFRLQGGAVDKTVGDLYRFRRSVS
                     LQGGVVTMESETVALAAELPAERAPRARAEMVELASSGIYIRAPSDYEPTEAEREQLR
                     KSEEAEARAKDRGKPKGAGAVTSKTD"
     gene            complement(7618..8466)
                     /locus_tag="VFV30_02150"
     CDS             complement(7618..8466)
                     /locus_tag="VFV30_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4846921.1"
                     /translation="MRSVTIRVPVAPGVAIAADVAGRQGAPTVILGHGGGQTRHSWDK
                     AERQLAEAGYYAINYDLRGHGDSDWSPDADYSLETRADDLAAVAAQGSRPFALVGASL
                     GGITAMVAASRGLEPAAIVLVDIVPKMSQKGVAKITAFMTAHPDGFASLEEAADAISA
                     YYPDRPRPKDLSGLNKNLRKGVDGRFRWHWDPRWMDSPRGDPHFLLDMMDAADWTDRV
                     PTLLVRGMKSDIVDDEGVEDLRRRIPHLEIADIRGAGHMVAGDKNDEFNAAVIDFLAR
                     VMPPQG"
     gene            8642..10630
                     /locus_tag="VFV30_02155"
     CDS             8642..10630
                     /locus_tag="VFV30_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4846922.1"
                     /translation="MRLRFNLFVLALLLITPLGALSGQPAAPAVPAAAPPAATSARPA
                     KIPTDAFTERSGLSDLKLSPDGSLIALRAVGDDGKVRIGFIDAQTRQGDRNLTVPDNY
                     TLEWIAWAGNKRLLVSLSQLGMVYDEEYRFTRLYVYDLDSRSFTFIGKKGMGIVGDDV
                     LHIDPDGQYVLLAMQRTIMDWPTVWHFPLDGTAEKSGKLIQGQRPNVWDWFADNAGTV
                     RMGIKYLESGAFEVLYRKTGADDFKTIARIDRKNADKGLWQVLRIVGGTDEGFVLQDD
                     GTGRQVLRRFNYATGQAGDTFYAQPGWDITEAWIGEDQQPIAAFYADDRDRVVWFDPK
                     MKSLQARLEKALKVEEVWIGSRARDDSRMLVWAGSEADPGMWFVYTAVGMRLDPLFAE
                     KPRLVVADMARPKPVEYTARDGTTIRGYLTLPPGRAAKGLPLIILPHGGPFGVRDKLD
                     FSSEVQFLANRGYAVLQPNYRGSGGYGEAFETLGDGEIGRKMQDDLDDGMDWAVAQGF
                     ADPKRVCVVGSSYGGYAALWAVIRNPDRYRCAASFAGVTDWNSQLRYDRNFFSRDNAK
                     KWKRRVAGADGFNLDEVSPVKQVARLTRPVLLAHGEKDTTVPFRQFKSLRDAAAKARI
                     PIDLLVFPDEGHGFDKSANEAKWLNQLEAFLTKHNPAD"
     gene            10698..12002
                     /locus_tag="VFV30_02160"
     CDS             10698..12002
                     /locus_tag="VFV30_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4846923.1"
                     /translation="MTRLFRLFAALLALIAAGPAPAEKPAEAPAETVYVRAGHLIEPS
                     TGEVLKDRLIRIDNGRVTAILPWAPPPKGAKLIDWSGLWVLPGLIDAHVHLADFLQTS
                     DPAEPLRRSAEETAYAGARNARLTLEAGFTTVHDVGSYRGTGNIELRNAINRGDIPGP
                     RINAVGGYITVPGGGGELTGLPAGMVVPPEMRMGVVTSPADVTAKVNGMFDAGADSIK
                     LIATGAVLTEGTEPGQIELSGEQIAAAVAAARARGSWVTAHAHGAEGIKLAIRNGARG
                     IEHASLIDDEGIALAKANGTYLVMDVYNGDFIAEVGRRDGWSAEILRKNDETTEVQRQ
                     GFTKAVRAGVKVVFGTDAGVFPHGLNARQFRYMVRLGMTPLQAIQSATTVAAEFLGWD
                     KDVGSLTPGHYADMIAVERNPLEDITALEQVRHVMKGGVVIR"
     gene            12067..>12284
                     /locus_tag="VFV30_02165"
     CDS             12067..>12284
                     /locus_tag="VFV30_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846924.1"
                     /translation="MKSSTLALALLASTAGLAAVAVSAQAPAEAVSTQTWIYAGRVIA
                     VPGQPARGATTIVVQDGRIQALHQGYPMA"
BASE COUNT         1856 a         4307 c         4218 g         1903 t
ORIGIN      
        1 gatcccgccc atggagtggt cggacttggg ctccggctcc gggttggtga cgaccacgcg
       61 ttccagccgg tcgcggaagt ggcggaccag ccagccgacc ggggatgcgc cgcgggcgat
      121 caggttgaac cgctcggccc ccagggcatc ggccacgtcg atcaggtcca gcgcggtctg
      181 ctcgaaatag ttgccctcca ctatctcggt catgccgaag ccggggcggt ccaccgcgac
      241 ggggcgcagg cccgcggcct gcagcgcggt gacatagctg cgcggcagat agcggctggt
      301 ggtggcggtg tggaggtgga gggtcgggat ccggtcggcc gggccgtagt cggcaaaggc
      361 gatccgcccg ccccgggtgg ggcgcggcag caggcgaacg ggctcgctgc tggcggcgag
      421 gtccaccccg ccgaccgact gcgccgccag catccgcgcc gccgtgccaa cctgcgccgc
      481 gatcaccgcc agcgcggtgg cgttgcccac ccccagcgcg gtgtagatca ccttgagttc
      541 cgccttcacc gtctcgaccg agtggccaag ctggcgggcc aactcctcgc gcgattggcc
      601 gagggcaaac agccgggcga tgctggcctg gcgcgggctg aggccgaaaa tctcgcgcaa
      661 ggggccctcg ctgtcgagct cgagcggctc gtccagcgcg ccgagtttca gcgcggcgcc
      721 gaccagcgcg ggctggcgcg ggtggccgct tttcgccagc gcctgcttca gcgcgctgcg
      781 ggcggtttca taggtcacgc cctcgatctc ggcggcggcg cgcagggtgc cggcctgggc
      841 cagccggcag accagccgct cctcgctggg ggtcaggcgg aacatgctgc gggcgcggtt
      901 gaagcggtcc tgccggggat cgagcgcggc cagccgcagc gcggcgtggc tgggcgcggc
      961 ggcggcgccc cgccccttca gccagcgcgc caccatcggc cagtgggcat agggccggac
     1021 cggcagcagc agcaccatca ccgggctcgc ctcggccgga tggagaaagc ggatcagcgg
     1081 gcgcggaccg ggatcgggca gttcgcattc cagctcggcg cgttcgctgt cggtgaaggc
     1141 ggcgggccag tcctccgcca gggtgccggc cgcggcgtca tggccgcaaa cctgccaggc
     1201 ctcggcaaag atcgcgaccg ggcgcagcgg cttggcggcg gtgctttcgg acagcgagct
     1261 gtcggccgcg agcgcgcggt tgatcgcctg ttccagctgc tcgaaatcgt cggccagcag
     1321 gccgtcctcg cccgtctcgg cgatcggatt gcgctcccct tcgatcatgc gcgacctgtc
     1381 ggtgcctccg gattcgccgg cgccgcaacg caccgacggc gggaacctaa cagaacgggc
     1441 gcggcaatgc gcgccctatt cgtcagagct ggaagctcgg ccgctcggcc aggcgggcgc
     1501 gccaggcggc gatcgcaggg cggcctgcgt tgacatcgac cttgacccac ccggcaaagt
     1561 ccagcgccac cccggcggtg atgtcggcca ggctgaaccg gtcaatcgcc acccactcgc
     1621 gcccggtcat gtgcgcttcg aaccggtcaa ggaagtgccc cagccgcgcc cggccgcgct
     1681 cggccagttc ggggatctgg gcatagttgt gcggccccgg cagggcgcgg tccttcatcc
     1741 ccttggcggt gttgcgcagc acctcggcga tggccatgaa gcactcgccc tcgatcttcg
     1801 aattccagct cgccaccgcg gccttttcca gcggggtgga gccaagcagc ggcgcctcgg
     1861 gaaaggccgc atcggcccaa gcggcgatcc cggcgttgtc ggtcagcacc gccccgtcgt
     1921 ccagcaccag cgccggcacc gtgcaggccg ggttgatcgc gcggaattcc ggggtcagct
     1981 gctcgccctt cgacaggtcg acgatcttca ccgcgtgggg gatccccttc tcggccagga
     2041 caatccgcgc ccggcgcggg ctgggggcag gcgtgcaatc gtagaatgtc ggcatcgaag
     2101 ggtccctttc tggtcgcgcg gtcagcctag cacctccgcc gccaatggcg accccgcccg
     2161 gcctgacgct acggaaacgc cttgactcaa ttgagtcatg ttatactatc acctttacca
     2221 acggagtcgc cccggcgcga ctcgggccaa ccggccgccg ggcgaccccc atggagggag
     2281 acgatgccat ggcctatgcc gataccaaca ccaccacccg caagctgggc accggcgccg
     2341 cggtcctgct gatcgaagcc ggggtcgccg ccgcgctggt caccggcctg gcgatgaccg
     2401 ggacgatcaa gcccgacacg atcatcgaca ccttcgacat tcccaagccc caggtgaccc
     2461 ccaccccgcc cccgccggac aagccccagg tccgccgcaa cgagagcacg atcgaccgcc
     2521 cggacgtgct gatcaagctg cccccggtga ccgacaccct gccggaagtg gagctcaagc
     2581 cctcgggcga caacggcaag gatacggtcg gcgacgtcac cttcccccag cccgaagtga
     2641 cgcccgatcc gccccagccg ccgcgcttca cccccaagcg cgcggtcccg cgcggcagca
     2701 cggccaaatg ggtcaccacc aacgattacc cggcctccga actgcgcgcc gaacaccagg
     2761 gcactacccg ctaccgcctg tcgatcgatg ccggcggcgc ggtgaccgat tgcaccgtca
     2821 cggccagcag cggctatccg gggctggacc gcgcggcctg cgaaaccgtg aagcggcggg
     2881 ccaagtttga acccgcgacc gatcacaccg gcgcccgcgc cccgggcagc ttcagcggtt
     2941 cggtgacctg gcagatcccc aaggaatgag gccgcgggcc tgaccaggcc gccccgcccg
     3001 gcacccccgc cgggcggggc aaccgcgtgg ccggaaagcg cggctaaccc cctttcaaga
     3061 agacccatgc ttgcctgacg ccgatcccgg cggtagtccc cgctgcaccg ggatccgtgc
     3121 aacctcccgg acaggcttcg gcccaagtgt tgccttttca gcagtccgcg tgtccgcgga
     3181 tggaaaggct atgagatgac cgctgccgcc ccttccccca gctttgccga tctggtccgc
     3241 ctgtccgcgc gggtcgggtg cctgtcgttc ggcgggccgg tgggccagat cgcgctgatg
     3301 caccgcgaat tcgtggaaga gcgcggctgg atcggcgagg acgagtatct ccacgcgctc
     3361 aatttctgcc acctgctgcc cgggcccgaa gcccagcagc tggccacctg gatcggctgg
     3421 cggctgcacg gggtgaaagg cgggctggcc gccgggctgc tgtttgtggt gccgggcgcg
     3481 ctggtcatgc tggcgctctc ggtgctctat gcccaggcag cgggcctccc ctggttttcc
     3541 gcgctgttcc tggggatcaa ggcggcggtg ctggcgatca ttgcccaggc cctgctgcgg
     3601 atcgccggac gcacgctgga caccgctttc aagcggggga ttgccgccgc ggcctttctg
     3661 gcgctggcct ttttcgccct gccctttccg ctggtcgtgc tgggcgcggg cctgatcggc
     3721 gcgctggcgg ccgtgcgccg cccggacctg ctggcgctga agccgctggc cgccgcgcgc
     3781 cccgccgcgc cgcgcccctg ggggcataca atccgcgccg tgctgatcgg cggggcgctg
     3841 tgggcggcgc cgctgctggc ggtgctggcg gtgctggggc gcggccacgt gctgtgggat
     3901 atcggcacct tcttttcgca attggcggtg gtgaccttcg gcggggccta tgcggtcctt
     3961 gcctatatgg gccagcaggc ggtgcaggat ttcggctggc tcagcgcgcg ggaaatggcc
     4021 gacgggctgg gcctggcgga aacgacgccg gggccgctga tcatggtcac gcagttcgtc
     4081 ggctatctgg ccgccttccg cgcgccggag ccgttcagcc cggtggtggc cggggtgatc
     4141 ggcgcggggc tgaccacctg ggtcaccttc gcgccgtgct tcgtctggat ctttgccctg
     4201 gccccgtggc tggaacggct ggagcacgcc cggcggctga aaggcgcgct cagcgcgatc
     4261 accgccgccg tggtcggcgt gattgccagc ctggcgctgt ggtttgcgct gcacgtgctg
     4321 ttcaccgatc tgatcccggc ccggctgggc ccggcgggct atgacctgcc ggtgctggcc
     4381 agcctcgatt ggcgggcggc gctgctggcg gcgctctctg ccgtgctgct gttccgcgcc
     4441 aggtggagcg tgctgaaagt cctgccgctg gcggcgctgg gcggattggt gctgggcact
     4501 ctcaccggtt cttaaccccg ccgtgttagg gcgcggagga atcgatcagg ggcaggaccc
     4561 atgcaactat ccgtcttccc ggccgagacc cgccaggcct tcgccgccgc ctatccggcc
     4621 accccggcca agctgcacca cagcctgctg ggccacccgc tgctgacgct ggacgcgctg
     4681 gccgccctgg ccgaggcgct gccggcacac gagatcgaat acaaccccgg ccagctgccg
     4741 atcgggatca agcccgaaga cgtgccgaaa agcgatctga ccgtgaccga gacgatccgc
     4801 aacatcggca aggccggatc ctgggtggcg ctgaagcgga tcgagcaggt gccggcctac
     4861 cgcgacctgc tccacgggat cctgggccag ctgcgcgaca tcgtcgagcc gcgcaccggc
     4921 gagatgatgc agctggaagg ctttgtcttc atcacctcgc ccggatcggt caccccgttc
     4981 cacttcgatc cggaacacaa catcctgctg caggtgatgg gccacaagac catgaccctg
     5041 ttcccggtgg aggacgaggc gatgttttcc gccgacatcc acgagaagtt ccacctgggc
     5101 ctgcaccacc gcaacctgcc ctggtacgat ggctatgccg aaaagggcac gccgatccac
     5161 atcggcccgg gcgaggcgat ccacgtgccg gtcaaggctc cgcactgggt gcagaacggc
     5221 gatcagccct cggtctcgct gtctatcacc tggcggtcgg actggtccta tgccgaggcc
     5281 gatgcccgcg ccttcaacca cgtgctgcgc aagattggcc tgcgcccggc cagcccccgg
     5341 cccttcccgg ggcgcaacct gggcaagtcg ctggcctggc gcgcaatccg ccgcctgcgc
     5401 ggtgcggctt agtccgtctt tgacgtaacc gcgccggcac ccttgggctt gccgcggtct
     5461 ttcgcccggg cttcggcctc ttccgacttg cgcagctgtt cgcgctcggc ctcggtcggc
     5521 tcgtagtcgc tcggcgcgcg gatgtagata ccgctgctgg ccagctcgac catttccgcc
     5581 cgggcccgcg gcgcgcgttc ggccgggagt tcggccgcaa gcgcgacggt ttccgattcc
     5641 atcgtcacca ccccgccctg cagcgaaacc gagcggcgga agcggtaaag atcgcccacc
     5701 gtcttgtcga ccgcgccgcc ctgcaggcgg aagccgcgcc cttcataggg cagcacgatc
     5761 gtgaccttgg ccgaccacca gtccggatag tcgaaggcat agggcgcgtc cttggcgatc
     5821 tcgccctcgc gggcgatgtc gagccgccag cccagccgcg atcggtcgat ctcgtaccag
     5881 cgggtgccga ggtccgaggt ccactccatc tgcgccgaac cggtcatggt caggatgaaa
     5941 tccccggttt cgggatcggt gcgggtcgcc accttgcccg gggaaatgaa atcgaaggca
     6001 tcgcgccaga ccttgcgcag ttcgcggttg cgatcggcct gcgaatagcc ggcgaacttc
     6061 tgccggacgc tggacccggc atcgccggaa aagcgcattt cgccggttac cgtggccggc
     6121 aggtccagcc ccttggaggc atcgaagctg aggctgaccg agctgaccgg ccgggtcagc
     6181 gccgggggca cgatcgccac caggcccgcg ccgtccgccg tcaccggcag gccgaagcgg
     6241 taattcgggg tatcgagccg gtcgagccgc tcatccccca ggcgggtgcc gtcgagccaa
     6301 tagcttttcc cgtcgatcgt cgcttcaacg atgacgtggt cgaaggcttc catggtcggc
     6361 agacgggcgc cgacgaaatc gccggattcg gtgttcacca gcaccggccg cgcctcgatc
     6421 cccagctctt tcagcagcgc gatcagcagc acggtcttgc ccttgcaatc gccgaaccgg
     6481 cgcgcccagg tctgctcggc gctggccggc acgaagccgc cggcgttcat ccccaggaac
     6541 aggtagcgga cctgatcctg caccagtttc agcgccagtt cggcccggcg ccggggatcg
     6601 ggtgtgatcg ccgcgatccg cgccgcctcg gccttgaccg ggctgtccgg cgcgagccgg
     6661 atcgcatcgg catagagcgg ggcgaaattg cgcgagaccg cctgccagtc cttgaattcg
     6721 gtgatctcca ccgcgttgac cacggcaaag cgcttgggcg cgccttcggg cgggctgggc
     6781 gtggtggtgt cggtgatgtc ggccaccagc tcgctgccgg tcgcggtctt cttcagccgg
     6841 ggctgcagca cgccgggaaa ggcgcgccac tgcatcggct tgtccttgtc ccacagcagc
     6901 cggacccgga accggccata ggtcgccccg tccgccgggc cggccaggta ctgcgaaaag
     6961 ccgcgcgcgg cggggtcgcg gcggaccacg gtataggcca gctcgatcac gtcgccgacc
     7021 cgcaggtcct cgggctggag cgtcgcggtc agcttgccgt ccagcatcgc gctttccatc
     7081 cgggtttcgc gctggatcac tttcagcgcg ctgccatcgc ccaggaggtc gatcaccttg
     7141 ccatcccgcc ggacccggta ggtgtggatc gtcagctgct ccagcgccgg gtcccagtcg
     7201 atcttcagcg cgcccgaatc gagcccgtgc gaggtgccta tcacccaggc cgcgttgtaa
     7261 tagctggcgt ccccgtcgcg cccgaagcgg acctggatat cgccgagcag gtcgatcgtc
     7321 gccgccccgt cggcatcgcg cggggccttg ggcagcgggg caatgtcgac ccaggccggg
     7381 gccggcgcga tcagcggacg gggaaagacc gccccggccg gtgcggcgct gggcgcggaa
     7441 gccggggagc tgtccggcag ggcggtgtcc ggcgctccgg ccccgaccag gccggtccag
     7501 gccgccgcca ccattacgat ccttgccagc cgcatcacat cttgcccctg tctcgcgcca
     7561 atccgcgtcg gggcaaaggg ttacggattt cggcggcaga gtcgagaggg tgctggctca
     7621 gccctgcggc ggcatgaccc gggccaggaa atcgatcacc gcggcgttga actcgtcgtt
     7681 cttgtcgccc gccaccatgt gcccggcgcc acggatgtcg gcgatctcca ggtgcgggat
     7741 ccggcggcgc agatcctcca ccccttcgtc gtcgacaatg tcgctcttca tgccgcggac
     7801 cagcagggtc ggcacccggt cggtccagtc ggcggcgtcc atcatgtcga gcaggaaatg
     7861 cggatcgccg cgcggcgaat ccatccagcg cgggtcccag tgccagcgga accggccgtc
     7921 cacgcccttg cgcaggttct tgttgaggcc cgagaggtcc ttcggccggg gccggtccgg
     7981 gtaataggcg gaaatggcgt cggccgcctc ttccaggctg gcaaagccgt cgggatgcgc
     8041 ggtcatgaag gcggtgatct tggccacgcc cttctggctc atcttgggga cgatatcgac
     8101 cagcacgatc gccgccggct ccagcccgcg cgaggccgcg accatcgccg tgatcccgcc
     8161 cagcgaggcg ccgaccagcg cgaacgggcg cgatccctgg gccgccaccg cggccagatc
     8221 gtcggcgcgg gtttccaggc tgtagtcggc gtcgggcgac cagtcgctgt cgccatggcc
     8281 gcgcaggtca tagttgatcg cgtaatagcc agcttcggcc agctggcgtt cggccttgtc
     8341 ccacgaatgg cgggtctgcc cgccgccgtg gcccaggatc acggtcggcg cgccctggcg
     8401 cccggcgaca tcggcggcaa tggcgacgcc gggggcaacg ggcacgcgga tggtgacgga
     8461 acgcatcgcg cgatgtggcc cggctgcgcg ggcgaggtca agcgggaatt taatcgcacg
     8521 attaaaaacg gatcgctgcc accatccttc ccgaaacgga tcaaatttcc gcaggagcca
     8581 ttgccctcta ttcaaagcgt tattagcctt tcgaatcgta atagtactac gaggcaagac
     8641 gatgcggctg cgcttcaacc tgttcgttct ggcgttactt ctgatcaccc cgctgggcgc
     8701 gctttcgggc cagccggctg cgccagcggt gcccgcagcc gccccgcctg ccgcaacctc
     8761 cgcccgtccc gccaagatcc cgaccgacgc cttcaccgag cgctccggcc tcagcgatct
     8821 caagctttcg cccgatggct cgctgatcgc gctgcgcgcg gtcggtgatg acggcaaggt
     8881 gcggatcggg ttcatcgatg cccaaacccg ccagggtgac cgcaacctga ccgtgccgga
     8941 caactacacg ctggaatgga tcgcctgggc cggcaacaag cggctgctgg tctcgctctc
     9001 gcagctcggc atggtctatg acgaggagta ccgcttcacc cggctctacg tctacgatct
     9061 ggacagccgc agcttcacct tcatcggcaa gaaggggatg ggcatcgtcg gtgacgatgt
     9121 gctgcacatc gatcctgacg gccagtacgt cctgctggcg atgcagcgca cgatcatgga
     9181 ctggcccacc gtctggcact ttccgctgga cggcacggcg gagaagtccg gcaagctgat
     9241 ccagggccag cgccccaacg tgtgggactg gtttgccgac aacgccggca cggtgcggat
     9301 ggggatcaag tacctcgaaa gcggcgcgtt cgaggtcctc taccgcaaga ccggggccga
     9361 cgatttcaag accatcgccc ggattgaccg caagaacgcc gacaagggcc tgtggcaagt
     9421 gctgcggatc gtcggcggca ccgacgaagg ctttgtcctg caggacgacg ggaccgggcg
     9481 gcaggtgctg cgccgcttca actatgccac cggccaggcc ggggatacgt tctacgccca
     9541 gcccggctgg gacattaccg aggcctggat cggcgaggac cagcagccga tcgccgcctt
     9601 ttatgccgac gaccgcgacc gggtggtgtg gttcgatccc aagatgaaat cgctgcaggc
     9661 ccgcctggaa aaggcgctga aggtggaaga ggtctggatc ggatcgcgcg cccgggacga
     9721 ttcgcggatg ctggtctggg ccgggagcga ggccgatccc ggcatgtggt ttgtctacac
     9781 cgcggtcggc atgcggctgg acccgctgtt cgcggaaaag ccgcggctgg tggtggccga
     9841 catggcccgg cccaagccgg tcgagtacac cgcccgcgat ggcaccacga tccgcggcta
     9901 cttgaccctg ccgccggggc gcgcggccaa gggcctgccg ctgatcatcc tgccgcacgg
     9961 cggcccgttc ggcgtgcgcg acaagctcga tttcagcagc gaggttcagt tcctggccaa
    10021 ccgcggctac gccgtgctgc agcccaatta ccgcggctcg ggcggctatg gcgaagcctt
    10081 tgaaacgctg ggcgacggcg agatcggccg caagatgcag gacgatctgg acgacggaat
    10141 ggactgggcc gtggcccagg gctttgccga tcccaagcgg gtctgcgtgg tcgggtcctc
    10201 ctatggcggc tatgccgcgc tgtgggcggt gatccgcaac ccggaccgct accgctgcgc
    10261 cgccagcttt gccggggtga ccgactggaa cagccagctg cgctatgacc gcaacttctt
    10321 ctcgcgcgac aacgcgaaga agtggaaacg ccgggtcgcc ggggcggacg gcttcaactt
    10381 ggacgaggtc agcccggtca agcaggtcgc ccggctgacc cgcccggtcc tgctggccca
    10441 cggcgaaaag gacacgaccg tgcccttccg gcagttcaaa tcgctgcgcg acgccgcggc
    10501 caaggcccgg atcccgatcg acctgctggt cttccccgac gaaggccacg ggtttgacaa
    10561 gagcgcgaac gaggccaagt ggctgaacca gctggaggcc ttcctgacca agcacaaccc
    10621 ggccgactga gcgggtaggc agcgggcctg caatcgatcg gttgcggcgg cgcggcggac
    10681 agggcacctt ggcggcgatg acacgcctgt tccgcctgtt cgccgcgctg cttgcgctga
    10741 tcgccgccgg tccggccccg gccgaaaagc ccgccgaagc gccggccgag acggtctatg
    10801 tccgggccgg ccacctgatc gagccgtcca ccggcgaagt gctgaaggac cggctgatcc
    10861 ggatcgacaa cggccgggtc accgcgattc taccgtgggc gccgccgccc aagggggcca
    10921 agctgatcga ctggtcgggc ctgtgggtgc tgcccgggct gatcgacgcc catgtccacc
    10981 tggccgattt cctgcagacc agcgatccgg ccgaaccgct gcgccgcagc gccgaggaaa
    11041 ctgcctatgc cggcgcgcgc aacgcgcggc tgacgctgga ggccgggttc accacggtcc
    11101 acgatgttgg gtcctaccgc ggcaccggca acatcgaact gcgcaacgcg atcaaccgcg
    11161 gcgacattcc ggggccgcgg atcaacgcgg tgggcggtta tatcaccgtg cccggcggcg
    11221 gcggggagct gaccggcctg cccgccggga tggtggtgcc gcccgaaatg cggatgggcg
    11281 tggtgaccag cccggccgac gtgaccgcca aggtcaacgg catgttcgat gccggggcgg
    11341 attcgatcaa gctgatcgcc accggcgcgg tcctgaccga agggaccgag ccggggcaga
    11401 tcgagctgtc gggcgagcag atcgccgccg ccgtggccgc cgccagggcg cggggcagct
    11461 gggtgaccgc ccacgcccac ggcgccgagg ggatcaagct ggcgatccgg aatggtgccc
    11521 gcgggatcga gcatgccagc ctgatcgacg acgagggcat cgcgctggcc aaggccaacg
    11581 gcacctatct ggtgatggac gtctacaacg gcgatttcat tgccgaagtc ggccgacgcg
    11641 acggctggtc ggccgagatc ctgcgcaaga acgacgagac caccgaagtc cagcgccagg
    11701 gctttaccaa ggccgtccgc gcgggcgtga aagtggtgtt cgggaccgac gccggggtct
    11761 ttccccacgg cctcaacgcc cgccagttcc gctatatggt gcggctgggg atgaccccgc
    11821 tgcaggcgat ccagtccgcc accacggtgg ccgccgaatt ccttggctgg gacaaggatg
    11881 tcggctcgct gaccccgggc cactatgccg acatgatcgc ggtggagcgc aatccgctgg
    11941 aggacatcac cgcgctggaa caggtccgcc acgtgatgaa gggcggcgtg gttatccgtt
    12001 gacgttgcgg ccccgccccc gccccgctag gcttcgcgca aagcaccatc cccgggagac
    12061 cctgtcatga aatccagcac ccttgctctt gccctgctcg cctccaccgc cgggctggcc
    12121 gccgtggcgg tgagcgccca ggccccggcc gaagcggtca gcacgcagac atggatttat
    12181 gccgggcggg tgatcgcggt gccgggccag ccagcgcgcg gggcgaccac gatcgtggtg
    12241 caggacggac ggatccaggc cctgcaccag ggctatccca tggc
//
LOCUS       DASUDV010000100         6606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1328457,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000100 DASUDV010000000
VERSION     DASUDV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328457"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..760)
                     /gene="pdeM"
                     /locus_tag="VFV30_02170"
     CDS             complement(80..760)
                     /gene="pdeM"
                     /locus_tag="VFV30_02170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX4846925.1"
                     /translation="MVPFSFAGEEMALAGPRALYWPREQALLVADLHLEKASFYARFG
                     QMLPPYDSRETLGQIADLLRRTGARRVLCLGDNFHDAEGADRLEPHAMGMLEALTRAA
                     DWVWVSGNHDLGRVPLGTAVDELELSGLVLRHEAVPGERRPELSGHFHPRLKVTARGR
                     RVVRPCAVKASGEAGERKLILPAFGALTGGMDAADPAIIAALQPARAVDALLGVQGKL
                     VQFPLWRG"
     gene            807..1676
                     /gene="hemF"
                     /locus_tag="VFV30_02175"
     CDS             807..1676
                     /gene="hemF"
                     /locus_tag="VFV30_02175"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX4846926.1"
                     /translation="MTDTATDWTPHTARARAWFESLRDRICAEFEAIEAEAGSPARFE
                     YLTWQREEEGNPDPGGGTRGVMKGQVFEKVGVNVSTVHGTFAPEFAKTIHGAGAENPG
                     FTATGISLVAHMANPHVPAVHMNTRFLTTSKAWFGGGGDLNPPIPYAEDTADFHAAFK
                     AACDAHGADYYERFKAWADDYFFIPHRGVHRGVGGIFYDHLDCADDAAFEAHFAFTRD
                     VGEAFLAVFPMLVRRRMGMDFTPAEKRQQLEWRGRYAEFNLVYDRGTLFGLKTGGNVD
                     AILMSLPPQAEWS"
     gene            1852..3942
                     /locus_tag="VFV30_02180"
     CDS             1852..3942
                     /locus_tag="VFV30_02180"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX4846927.1"
                     /translation="MLRQYELVERVLAYDPDADEAMLNRAYVYTVQKHGSQKRASGDP
                     YFSHPVEVAGLMTELKLDQETIVTALLHDTVEDTLATPEEIERLFGPEIARLVDGVTK
                     LSKIEAMGENERAAENLRKFFLAMSEDIRVLLVKLADRLHNMRTLHFIKSEEKRRRIA
                     RETMDIYAPLAERVGMYEYMREMQLLAFEQLEPEAYATITGRLAQIRSSEGGQVDAIA
                     LAIKQALAEAGLKVEVSGREKHPYSIWKKMAERHVSFEQITDIMAFRVITESPQDCYG
                     ALGVLHTTWQMIPGRFKDYISTPKSNGYRSLHTALIYENSMRMEVQIRTREMHRLNEF
                     GLAAHWAYKHGARPDGQVGWLRDLIEIVDASHDAEELLEHTKLAIYQDRIFAFTPKGA
                     LHQLPKGSTPVDFAYAVHSDLGSFAVGAKINGRHMPLRTVLANGDVVEIIKSRNASPQ
                     LAWLGFVVTGKARAAIRRAVRAKERAEVAAIGRKLFEQIVERLPSKIGKKAVKDAVKR
                     LGLSSEDELMVAIGTAKLEDRAVMEALVPGSSKQLSEPEHWPKQERAISIRGLTAGMA
                     FHLADCCHPVPGDRIVGLRHAGKGVEVHAIDCLTLASGVDADWLDLSWGERSTGAVGR
                     IRMVLYNRPGTLAEAAGIFAGNRANIAALENASRDELFSTYEVEIEVTDLAHLTRIVS
                     ALRASDAVAEAERI"
     gene            4072..4431
                     /locus_tag="VFV30_02185"
     CDS             4072..4431
                     /locus_tag="VFV30_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4846928.1"
                     /translation="MAIVGIDHVQLAMPPGEEEAARGFYSGLLGLPEVPKPPALAARG
                     GCWFEGPSVKLHLGVEADFRAARKAHPGLLVDDLAALVAVLQAAGVSATPGDPVEGRP
                     RCFVSDPFGNRIELMQA"
     gene            complement(4428..4742)
                     /locus_tag="VFV30_02190"
     CDS             complement(4428..4742)
                     /locus_tag="VFV30_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEX4846929.1"
                     /translation="MTERIAHTGPLWRWTGASGTGSWHFLTIDGDAGEALSGTALMRR
                     MERSLGGFGSLKVTATIGDSTFKTSLFPSKELGWLLPVKASVRKAEGIGAGDEVAVVL
                     EL"
     gene            complement(4739..5797)
                     /locus_tag="VFV30_02195"
     CDS             complement(4739..5797)
                     /locus_tag="VFV30_02195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4846930.1"
                     /translation="MRKTPLAIAAVLAVSASAVLAQASANQAAGRTQPSPLAPAEIVA
                     KAPKADWTAIAASDLLVMDLAPDARGKARRVVIQLMPPPFSQGWIGNIRKLTAARFWD
                     GTAINRVQDNYVVQWGDPNAEDKAKAKALPADLVTVPESDYVTPVKWLRHDDEISHSY
                     WLSPGVSRIEQRSAYAQAEGFHWGWPVAGDLPGMDDLKPGIGGPPHPMDHPKQVWPVH
                     CYGMVGVGRDLSPNTGTGAELYTVIGHAPRHLDRNIALVGRVIEGVEHLSSLPRGKGP
                     LGFYDLKQGDRRVPIRAIRLGSEVKGLSAYEYLSTESGSFAQYADARANRRDAFFIRP
                     AGGADICNIPVPVRRVPV"
     gene            complement(5855..6259)
                     /locus_tag="VFV30_02200"
     CDS             complement(5855..6259)
                     /locus_tag="VFV30_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX4846931.1"
                     /translation="MTRPFGPAPSLSEIEALARAALARLPEPFAAHLDGVLLAVEDFA
                     DEETLAALGIADPFELTGIYEGWPVGEKHSAPSGTLPDRIRLFRSPILDEWIARGDET
                     LEHLVSHVLIHEVGHHFGLSDDDIDALEAAAD"
     gene            6339..6429
                     /locus_tag="VFV30_02205"
     tRNA            6339..6429
                     /locus_tag="VFV30_02205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6373..6375,aa:Ser,seq:gga)
BASE COUNT         1130 a         2266 c         2199 g         1011 t
ORIGIN      
        1 tacccttggg gtggttgggc cgggggagcg ggccggtgat gccaagccga tcgcggaggc
       61 gatcggcgca caaggcaaac tacccccgcc acagcgggaa ctgcaccagt tttccctgga
      121 cccccagcag cgcgtcgacc gcgcgggccg gttgcagcgc ggcgatgatc gccgggtcgg
      181 cggcgtccat cccgccggtc agcgcgccga aggcgggcag gatcagtttg cgctcgcccg
      241 cctcgcccga ggccttcacc gcgcaggggc gcaccacccg gcggccgcgc gcggtcactt
      301 tcaggcgagg gtggaaatgg ccggacagtt cggggcgccg ttcgcccggc acggcctcgt
      361 gccgcaggac cagcccggac agctccagct cgtccaccgc cgtgccgagc ggcacccgcc
      421 ccagatcgtg gttgcccgaa acccagaccc agtcggcggc gcgggtcagc gcctccagca
      481 tgcccatcgc gtgcggctcc agccggtcgg ccccttcggc gtcgtggaaa ttgtcgccca
      541 ggcacagcac ccggcgtgcg ccggtccgcc gcagcaggtc ggcgatctgg cccagcgtct
      601 cgcggctgtc atagggcggg agcatctgcc cgaagcgggc atagaagctg gccttttcca
      661 gatgcaggtc ggccaccagc agcgcctgct cgcgcggcca gtacagcgcg cggggccccg
      721 ccagcgccat ctcctcaccc gcgaacgaaa agggaaccat ggctttccct tgccggatcg
      781 ggattcgctt ggcaagggca ctggccatga ccgacaccgc caccgactgg accccgcaca
      841 ccgcccgcgc ccgcgcctgg ttcgaaagcc tgcgcgaccg gatctgcgcc gaattcgaag
      901 cgatcgaggc cgaagccggc agtccggcgc ggttcgaata cctcacctgg cagcgcgagg
      961 aagagggcaa ccccgatccc ggcggcggca cccgcggggt gatgaagggc caggtcttcg
     1021 aaaaggtcgg ggtcaatgtc tcgaccgtcc acggcacctt cgcccccgaa ttcgccaaga
     1081 cgatccacgg tgccggggcg gagaatcccg gcttcaccgc cacggggatc agcctggtcg
     1141 cgcacatggc caacccccac gtgccggccg tgcacatgaa cacccggttc ctcaccacca
     1201 gcaaggcttg gttcggcggc ggtggcgatc tcaacccgcc gatcccctat gccgaggata
     1261 ccgccgactt ccacgccgcc ttcaaggccg cctgcgacgc ccacggggcc gactattacg
     1321 agcggttcaa ggcctgggcc gacgactatt tcttcatccc ccaccgcggc gtgcaccgcg
     1381 gggtgggcgg gatcttctac gatcacctgg actgcgccga cgatgctgcc ttcgaagccc
     1441 acttcgcctt cacccgcgac gtgggcgagg cgttcctggc ggtgttcccc atgctggtcc
     1501 gccgccggat gggcatggac ttcaccccgg ccgaaaagcg ccagcagctg gaatggcgcg
     1561 ggcgctatgc cgaattcaac ctggtttacg accggggcac gctgttcggg ctgaagaccg
     1621 gcgggaacgt cgatgcgatc ctgatgagcc tgccgccgca ggccgaatgg tcctgatcgg
     1681 ccgctccgcc gggttaggcc aatctttgca attgcggggt taaaggcgag tcggacgggg
     1741 cagcatctgt caccaccgcc gctgccccgt tcgattcccg ccgccgatcc gcaccctgcc
     1801 agcgcctttg ggcttgccct tggcggcgca atcctttctt ctggccggga aatgctgcgc
     1861 caatacgaac ttgtcgagcg ggtgctcgcc tatgatcccg atgcggacga ggcgatgctc
     1921 aaccgcgcct atgtctacac cgtccagaaa cacggctcgc aaaagcgtgc cagcggcgat
     1981 ccctatttca gccacccggt cgaggtggcc gggctgatga ccgagctcaa gctcgaccag
     2041 gaaaccatcg tcaccgccct gctccacgat acggtggagg acaccctggc caccccggaa
     2101 gagatcgagc gcctgttcgg gcccgaaatc gcccggctgg tcgacggggt gaccaagctc
     2161 tccaagatcg aggcgatggg tgagaacgag cgcgcggccg agaacctgcg caagttcttc
     2221 ctggcgatga gcgaggacat ccgcgtcctg ctggtcaagc tggccgaccg gctgcacaac
     2281 atgcgcacgc tccacttcat caagagcgag gagaagcgcc gccggatcgc gcgcgagaca
     2341 atggatatct atgccccgct cgccgaacgg gtggggatgt acgaatacat gcgcgaaatg
     2401 cagctgctgg ccttcgagca gctggaaccc gaagcctatg ccacgatcac cgggcggctc
     2461 gcccagatcc gctcgagcga gggcgggcag gtcgatgcga tcgccctggc gatcaagcag
     2521 gccctggccg aagccgggct gaaggtggag gtttcgggcc gcgaaaagca cccctattcg
     2581 atctggaaga agatggccga acgccacgtc agcttcgaac agatcaccga tatcatggcc
     2641 ttccgggtga tcaccgaaag cccgcaggac tgttacggcg cgctgggcgt gctgcacacc
     2701 acctggcaaa tgatccccgg gcgattcaag gactacatct ccacgccgaa gtcgaacggc
     2761 taccgctcgc tccacaccgc gctgatctat gaaaactcga tgcggatgga agtgcagatc
     2821 cgcacccgtg aaatgcaccg gctcaacgaa ttcggccttg ccgcgcactg ggcctacaag
     2881 cacggcgccc ggcccgacgg gcaggtcggc tggctgcgcg acctgatcga gatcgtcgat
     2941 gccagccacg acgccgaaga gctgctcgaa cacaccaagc tggcgatcta ccaggaccgg
     3001 atcttcgcct tcacgcccaa gggcgcgctg caccagctgc ccaagggctc gaccccggtc
     3061 gatttcgcct atgccgtcca ttcggatctc ggctcgttcg cggtcggggc caagatcaac
     3121 ggccggcaca tgccgctgcg cacggtgctg gccaacggcg acgtggtgga aatcatcaag
     3181 agccgcaacg cctcgccgca gctggcctgg ctgggctttg tggtgaccgg caaggcccgc
     3241 gccgcgatcc gccgcgcggt ccgcgccaag gagcgcgccg aagtggccgc gatcgggcgc
     3301 aagctgttcg aacagatcgt cgagcggctg cccagcaaga tcggcaagaa ggcggtcaag
     3361 gacgcggtca agcggctggg cctgtccagc gaggacgagc tgatggtggc gatcggcacc
     3421 gccaagctgg aagaccgcgc ggtgatggag gccctggttc cgggcagctc caagcagctg
     3481 tccgaacccg aacactggcc aaagcaggaa cgcgcgatct cgatccgcgg gctgaccgcg
     3541 ggcatggctt tccaccttgc cgattgctgc cacccggtgc cgggcgaccg gatcgtcggc
     3601 ctgcgccacg ccggcaaggg ggtggaagtc cacgcgatcg actgcctgac cctggccagc
     3661 ggggtcgatg ccgattggct cgacctgtcg tggggcgaac gcagcaccgg cgcggtcggg
     3721 cggatccgga tggtgctcta caaccgcccc ggcaccctgg ccgaagccgc cgggatcttc
     3781 gccggcaacc gcgccaacat cgccgcgctg gaaaacgcca gccgcgacga gctgttttcg
     3841 acctacgagg tggaaatcga agtcaccgac ctggcccacc tgacccgcat cgtcagcgcc
     3901 ctgcgcgcca gcgatgccgt ggccgaggca gagcggatct agcggttgcg gaggcgtagc
     3961 cgaagcagag cggagagcaa ctgatgcggt tgcggaggca cagccgcagc agagcggaga
     4021 gcaactgatg cggttgcgga ggcgtagccg cagcagagcg gagatcggct gatggcgatc
     4081 gtcggtatcg accacgtcca gttggccatg ccgcccggcg aggaagaagc ggcgcgcggg
     4141 ttctattccg gcctgctggg gctgcccgaa gtgcccaagc ctccggccct cgcggcgcgc
     4201 ggcgggtgct ggttcgaagg cccgtcggtc aagctgcacc ttggcgtgga ggccgatttc
     4261 cgcgccgcgc ggaaagccca tcccggactg ctggtcgatg atctggccgc cttggtggcg
     4321 gtgctgcaag cggccggtgt atccgccacg cccggcgatc cggttgaagg gcggccccgc
     4381 tgtttcgtca gcgatccgtt cggcaaccgg attgaactga tgcaggccta gagctccagc
     4441 accacggcca cttcgtcccc cgcgccgatc ccttcggcct tgcgcacgct ggccttgacc
     4501 gggagcagcc agcccagttc cttcgagggg aacagcgagg tcttgaacgt gctgtccccg
     4561 atcgtcgcgg tgactttcag ggagccgaac ccgccaagcg atctctccat ccgccgcatc
     4621 agcgcggtgc cggacagcgc ctcgcccgcg tctccgtcga tggtcaggaa atgccagctg
     4681 ccggtgccgc tggcgccggt ccagcgccac agcgggccgg tatgtgcgat acgttcggtc
     4741 aaacgggcac gcgccgcacc gggaccggga tgttgcagat atccgccccg cccgccggcc
     4801 ggatgaagaa ggcgtcgcgg cggttggcgc gggcgtcggc gtattgggca aagctgccgc
     4861 tttcggtgga gaggtattcg taggccgaca ggcccttgac ctcgctgccg aggcggatcg
     4921 cgcggatcgg cacacgtcgg tcaccctgct tgagatcata gaaccccagc ggccccttgc
     4981 cgcgcggcag gctggacaag tgctcgaccc cctcgatcac ccggccgacc agcgcaatgt
     5041 tgcgatcgag atgccgcggg gcatggccga tgacggtata gagctcggcc ccggttccgg
     5101 tgttgggcga aaggtcccgc ccgacgccga ccatgccata gcagtggacg ggccatacct
     5161 gctttggatg atccatagga tgcggagggc cgccgatccc gggctttaga tcatccatcc
     5221 cgggcaggtc tccggcaact ggccaacccc agtgaaagcc ttccgcttgg gcgtaggcgc
     5281 tgcgctgctc gatccgtgaa acacccgggg aaagccagta cgaatgggag atttcgtcat
     5341 cgtggcggag ccatttgacc ggcgtgacat aatcactctc cggcaccgtc accaaatccg
     5401 ccggcagagc cttcgccttg gccttgtcct cggcgttggg atcgccccac tggaccacgt
     5461 aattgtcctg gacccggttg atcgccgtcc cgtcccagaa ccgcgcggcg gtcagcttgc
     5521 ggatgttgcc gatccagccc tggctgaacg gcggcggcat cagctggatc accaccctgc
     5581 gggccttgcc gcgggcatcc ggggcaaggt ccatcaccag cagatcggac gcggcaatcg
     5641 ccgtccagtc cgccttgggg gccttggcca cgatctcggc gggggccagc ggcgagggct
     5701 gtgtgcgccc cgcggcctgg ttggccgagg cctgggcgag cacggccgag gcactgacgg
     5761 caagcacggc ggcgatagca aggggggttt tgcgcatggc cggagtgtga agggtttggc
     5821 gcggggtttc aatccttccc gtgccgggag ggacctagtc cgccgccgcc tccagcgcgt
     5881 cgatatcgtc atccgacagg ccgaaatgat gccccacctc gtggatcagc acatggctca
     5941 ccaggtgttc cagcgtctcg tccccgcggg cgatccattc gtccaggatc ggcgagcgga
     6001 acagccggat ccggtcgggc agggtgccgc tcggcgcgga gtgtttttcc cccaccggcc
     6061 aaccctcgta gatcccggtc agctcgaacg gatcggcaat gcccagcgcg gccagggtct
     6121 cctcgtcggc aaagtcctcc accgccagca gcacgccatc gaggtgcgcg gcaaagggtt
     6181 cgggcagccg cgccagcgcg gcgcgggcca gggcttcgat ttcggacagg ctgggcgcgg
     6241 ggccaaaggg acgggtcatg ggccccagca tagccgccgc cgcgacgctt gcctatcgcc
     6301 ccccatcccg ctagggcgcg cgactatcca gtgcatgcgg agcggtggcc gagtggtcga
     6361 aggcgctcgc ctggaaagtg agtatacgtc aaaagcgtat cgagggttcg aatccctccc
     6421 gctccgccac aaacccccag cacctgtctc agccgaatgg gggcaatcgg cttcagccgg
     6481 attgccgatc ccctgcgggt agggcaccaa cgggtccatg attacgccat cgccatcgcc
     6541 ggaccggccc gccatgcggc tgggcgtctg ctattatccc gaacactggc ccgaagcgct
     6601 gtgggc
//
LOCUS       DASUDV010000101         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_201990,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000101 DASUDV010000000
VERSION     DASUDV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201990"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VFV30_02210"
     CDS             complement(<1..1054)
                     /locus_tag="VFV30_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4846932.1"
                     /translation="MKGLAPLNAKLLRDLWRLRAQALAIALVMAAGVGMVVMSYGMIR
                     SLEATRAAYYQRFALADLWVPLRRAPDSLARDLRAVPGIAGLESRVSAAATLDIAGVA
                     EPVSARLHSLPMALNRLVLRSGRLPRPDRPDEVVASELFVKAARIELGTRLPALVHGK
                     RIELTVVGTALSPEYVYAVAPGQIFPDNRRFAVLWMGREPLAAALDMRDGFNELLVNL
                     APGADADETVRRLDLAAAPYGGTGAFGRDLLVSERFVSSEIEQLRTTVEILPPIFLGV
                     AAFLLNILLARLVDTEREVIGLLKAFGYRNGAIVRHYGALALLLSVGGVVLGWALGAW
                     MGRGIAGIYQDFYTFPF"
     gene            complement(1051..1764)
                     /locus_tag="VFV30_02215"
     CDS             complement(1051..1764)
                     /locus_tag="VFV30_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4846933.1"
                     /translation="MSEGAAIYRTRGLTKSYGSGDTAVHALRGVDLVVHRGEMLVLLG
                     PSGSGKSTLLNILGGLDSATSGQAWFDDLEITALDADGLTEYRRRSIGFVFQFYNLIA
                     SLTARENVQLITDIAENPMSPEQALALVGLEDRLDHFPAQLSGGEQQRVAIARAIAKR
                     PEVLLCDEPTGALDSKTGARVLAALDQVNREVGTTVFIITHNAVIADMADEVIRFADG
                     AIASHERNGARRSPDELVW"
     gene            complement(1761..>2082)
                     /locus_tag="VFV30_02220"
     CDS             complement(1761..>2082)
                     /locus_tag="VFV30_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4846934.1"
                     /translation="TALEALRKIALARLEGTQVVLPEIGETYELPAGAPARRTRTGRT
                     DLSQALGRDWQNAYAQLATSLKHDLAAIKDARQREEAIAAMRQVLDSYTRFRADKHGK
                     ARRR"
BASE COUNT          352 a          714 c          703 g          313 t
ORIGIN      
        1 ggaacgggaa ggtgtagaaa tcctggtaga tcccggcaat cccgcgcccc atccaggcac
       61 ccagggccca gcccagcacc accccgccca ccgaaagcag cagcgccagc gcgccatagt
      121 ggcgcacgat cgccccgttg cgatagccga atgccttgag cagcccgatc acttcgcgct
      181 cggtatcgac cagccgggcg agcaggatgt tgagcaggaa ggcggcaacg ccaaggaaga
      241 tcggcggcag gatctccacc gttgtgcgca gctgttcgat ctcgctcgaa acgaagcgtt
      301 ccgaaaccag cagatcgcgc ccgaaggccc cggttccgcc atagggcgcg gcggcgagat
      361 cgagccggcg caccgtttcg tcggcatcgg cgccgggggc caggttgacc agcagttcgt
      421 tgaagccatc gcgcatgtcg agcgcggcgg cgagcggttc gcgccccatc cacaggaccg
      481 cgaagcggcg attgtcgggg aagatctgcc ctggcgccac ggcatagaca tattcgggcg
      541 aaagcgcggt gccgaccacc gtcagctcga tccgcttgcc gtggaccagc gcgggcaggc
      601 gcgtgcccaa ttcgatccgg gccgccttga cgaacagttc gctggcgaca acctcgtccg
      661 gccggtcggg gcgcggcagc cggcccgaac gcagtaccag ccggttgagc gccatcggca
      721 gcgagtgaag ccgcgccgaa accggctctg ccaccccggc gatgtcaagc gtggcggcgg
      781 cgctgacccg gctttccaga ccggcaatgc cgggcaccgc gcgcagatcg cgggccaggc
      841 tatcgggcgc gcggcgcagc ggcacccaca gatcggccag ggcaaagcgt tggtaatagg
      901 ccgcgcgcgt cgcctcgagg ctgcggatca tgccatagga catgaccacc atccccaccc
      961 ccgcagccat gaccagcgcg attgccagtg cctgggcacg cagccgccac agatcgcgca
     1021 gcagcttggc gttgagcggg gccagcccct tcaccagacc agctcgtccg gcgaacggcg
     1081 cgcgccattg cgttcgtggc tggcgatcgc gccatcggca aagcggatca cttcatcggc
     1141 catgtcggcg atcaccgcgt tgtgggtgat gatgaacacc gtggtgccga cctcgcggtt
     1201 gacctgatcg agcgcggcca gcacccgcgc cccggttttg ctgtcaagcg cgccggtagg
     1261 ctcgtcgcac agcagcactt cgggacgctt ggcgatcgcc cgggcaatcg ccacgcgctg
     1321 ctgttcgccg ccggaaagct gggccgggaa atgatcgagc cggtcctcca gcccgaccag
     1381 cgccagcgcc tgttcggggc tcatcggatt ttcggcaata tcggtgatca gttgcacgtt
     1441 ctcgcgcgcg gtgagcgagg cgatcaggtt gtagaactgg aacacgaagc cgatcgagcg
     1501 gcgccggtat tcggtcagcc catcggcatc gagcgcggtg atctccagat cgtcgaacca
     1561 cgcctggccc gatgttgcgc tgtcgagccc gcccaggatg ttgagcaggg tcgacttgcc
     1621 cgatcccgaa gggcccagca gcaccagcat ttcgccgcgg tggaccacca ggtctacccc
     1681 gcgcagggca tgaaccgcgg tatccccgct gccataggac ttggtcaatc cccgggtgcg
     1741 gtagatcgcg gcaccttcgc tcaccggcgg cgggccttgc cgtgcttgtc cgcgcgaaag
     1801 cgggtgtagc tgtcgagcac ctggcgcatc gcggcaatcg cctcttcgcg ctggcgggcg
     1861 tccttgatcg cggccagatc gtgcttgagc gaggttgcca gctgggcata ggcattctgc
     1921 caatcgcgcc ccagggcctg cgacagatcg gtgcgcccgg tccgggtgcg gcgggccggg
     1981 gcgccggcgg gcagttcata ggtttcaccg atttcgggca gcaccacctg ggtgccctcg
     2041 agccgggcca gggcaatctt gcgcagcgcc tcaagcgcgg tc
//
LOCUS       DASUDV010000102         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_378645,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000102 DASUDV010000000
VERSION     DASUDV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378645"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFV30_02225"
     CDS             complement(<1..876)
                     /locus_tag="VFV30_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX4846935.1"
                     /translation="MRATQAFIEQTGGPEVIQWREVDLPAPGPGEVLIRHEAVGLNYI
                     DTYFRTGLYPAALPTGLGMEAAGVVEAVGDGVTHLKPGQRAVTFGAIGTYATARIAQA
                     MQYLPLPDGIDSQTAAAVFLKGATVEALAERCAPLEAGEWALVPAAAGGVGQLLVQWL
                     KARGVRVIGTVGSEAKIALARACGAEEVFLSGDPELADKVRAATGGAGVAVSYDGVGA
                     ATWEASLKSVRRRGTIVSFGNASGPVTGVNLGVLAQHGSLFVTRMTLFDYYREPAEAL
                     AGAAKLWDMVGSGKLT"
     gene            complement(955..1029)
                     /locus_tag="VFV30_02230"
     tRNA            complement(955..1029)
                     /locus_tag="VFV30_02230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(995..997),aa:Gln,seq:ttg)
     gene            complement(1082..1933)
                     /gene="yghU"
                     /locus_tag="VFV30_02235"
     CDS             complement(1082..1933)
                     /gene="yghU"
                     /locus_tag="VFV30_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEX4846936.1"
                     /translation="MADTEYTPPPVWTWNKDNGGQFAAINRPTAGAQKEKELPVGEHP
                     FQLYSLGTPNGQKVTILFEELLAAGHSGAEYDAWLVNIREGDQFGSGFVALNPNSKIP
                     ALLDRSGPEPFRVFESGAIMLHLAEKFGAFLPTDAKGRAECLSWLMWQMGSAPFIGGG
                     FGHFYAYAPIKIEYAIDRYSMETKRLFDVANNRLAESRFLAGDDYTIADMATYAWFGL
                     LYRGLAYNDSAKFLSLDEYTHVGRWVGEIGERPAVKRGQKVNLPTGLIERHSASDFDA
                     LETAAAE"
     gene            2090..2680
                     /locus_tag="VFV30_02240"
     CDS             2090..2680
                     /locus_tag="VFV30_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX4846937.1"
                     /translation="MTEITNDSPKLHPAVQEFVLRWGDLGGQWGVNRSVAQIQALLIL
                     SERPLNAEEIAEKLGMARSNVSNSLKELLVWKLIHRVPVLGDRRDFYAAETDMWQMAN
                     KVAQGRKEREIDPMLAAIDAAMAQAGTGGPLHPVVRERLGEMHGFIHTVEGWYRQMLD
                     VPPAQIMRLIKLGAKVVGLLKFVGGKGDKADRGGDG"
     gene            2787..>3270
                     /locus_tag="VFV30_02245"
     CDS             2787..>3270
                     /locus_tag="VFV30_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX4846938.1"
                     /translation="MAATPVPAELADLRFSALLGAAGWARLPAAVRARFAHRCAPGVS
                     VSYAGHVAECRMSTAGWLLAQACRLIGAPLPLDRGGGLAAVVTVTEHAPSGGQTWTRI
                     YARPGGFPQVIHSAKRFAGPTGLEEYLGLGFGIALRLEAGEDRIGFLSDHYFLRLGPV
                     R"
BASE COUNT          520 a         1078 c         1146 g          526 t
ORIGIN      
        1 ggtcagcttg ccgctgccga ccatgtccca cagcttggcc gcgccggcca gcgcctcggc
       61 cggctcgcgg taatagtcga acagggtcat ccgggtgacg aacagcgagc cgtgctgggc
      121 cagcacgccc aggttcacgc cggtaaccgg gccgctggcg ttgccgaacg agacgatcgt
      181 cccgcgccgc cggaccgatt tgagcgaggc ctcccaggtg gccgcgccga cgccgtcata
      241 gctgaccgcg acgcccgcgc cgccggtcgc cgcgcggacc ttgtcggcca gctccggatc
      301 gccggacagg aacacctctt cggccccgca tgcgcgggcc agcgcaatct tggcctcgct
      361 gccgaccgtc ccgatcaccc gtaccccgcg cgccttcagc cattgcacca gcagctgccc
      421 gaccccgccc gcggcggccg ggaccagcgc ccattcgccg gcctccagcg gcgcgcagcg
      481 ctcggccagg gcctcgaccg tcgcgccttt caggaagacg gcggcggcgg tctggctgtc
      541 gatcccgtcg ggcagcggca ggtactgcat ggcctgtgcg atccgggcgg tcgcataggt
      601 cccgatcgcc ccgaaggtga ccgcgcgctg gccgggcttc aggtgagtga ccccgtcgcc
      661 gacggcctcg accactccgg ccgcttccat gcccagcccg gtcggcaggg cggcggggta
      721 aagcccggtg cggaaatagg tgtcgatata attgagcccc accgcctcgt gccggatgag
      781 cacttcgccc gggccgggcg cgggcaggtc cacctcgcgc cactggatca cttcgggtcc
      841 gccggtctgt tcgataaagg cctgagtcgc gcgcatgggt ctctccttac cggccagcct
      901 agcggaccgg gaatgcggtg caaacggttt ggggagaagc ctgagcttgc gtcctggctg
      961 gggcggaagg attcgaacct tcgcatggcg gtaccaaaaa ccgctgcctt accgcttggc
     1021 tacgccccag caggagcgcg gcctataaat aggccgcgcg cgcttgggaa gggggcaatt
     1081 ctcactctgc cgcggcggtt tccagcgcgt cgaaatcgct ggccgaatgg cgttcgatca
     1141 ggccggtcgg caggttgacc ttctgcccgc gcttcaccgc cgggcgctcg ccgatttcgc
     1201 cgacccagcg gccgacgtgg gtgtattcgt cgagcgagag gaacttggcc gagtcattgt
     1261 aggccagccc gcgatagagc aggccgaacc aggcataggt cgccatgtcg gcgatggtgt
     1321 aatcgtcccc ggccaggaac cggctttcgg ccaggcggtt gttggcgaca tcgaacagcc
     1381 gcttggtttc catcgaatag cggtcgatcg cgtattcgat cttgatcggc gcataggcat
     1441 agaagtggcc aaagccgccg ccgatgaacg gcgcgctgcc catctgccac atcagccacg
     1501 acaggcattc ggcccggccc ttggcatcgg tgggcaggaa ggcgccgaac ttttccgcca
     1561 ggtgcagcat gatcgcgccg ctctcgaaga cgcggaacgg ctccgggccc gagcggtcga
     1621 gcagggccgg gatcttggaa ttggggttga gcgcgacgaa gcccgagccg aactggtcgc
     1681 cttcgcggat gttgaccagc caggcatcgt attcggcccc gctgtggccg gcggcgagca
     1741 gctcttcgaa caggatcgtc accttctggc cgttcggcgt gccgagcgaa tagagctgga
     1801 agggatgctc gcccacgggg agctccttct ccttctgggc cccggcggtg ggccggttga
     1861 tcgcggcgaa ctggccgccg ttgtccttgt tccaggtcca gaccgggggc ggggtgtatt
     1921 cggtgtcggc catggggcat ccttctgttc gggtctgtcg gttgcagaaa gaaatggacc
     1981 gcgccgcggg gccggtcaag caaccaagcc ttggcggggc aacagcccgg tttggggccc
     2041 cgtgaggatg cgggttgaca ggaggcgctg aaagacagat atttctgtca tgacagaaat
     2101 tacgaacgat tcgccaaagc tccaccccgc ggtgcaggaa ttcgtcctgc gctggggtga
     2161 tctgggcggg cagtgggggg tgaaccgttc ggtcgcgcag atccaagcgc tgctgatcct
     2221 gtcggaacgg ccgctcaacg ccgaggagat cgccgagaag ctgggcatgg cccgctccaa
     2281 cgtgtcgaac agcctgaagg aactgctggt ctggaagctg atccaccgcg tcccggtgct
     2341 gggcgaccgg cgcgacttct acgccgccga gaccgacatg tggcagatgg ccaacaaggt
     2401 tgcccagggc cgcaaggagc gcgagatcga cccgatgctg gcggcgatcg acgcggccat
     2461 ggcccaggcc ggcaccggcg ggccgctgca cccggtggtg cgcgagcggc tgggcgagat
     2521 gcacggcttc atccacaccg tggaaggctg gtaccgccag atgctggacg tgccgccggc
     2581 ccagattatg cggctgatca agctgggggc caaggtggtc ggcctgctca agttcgtcgg
     2641 cggcaagggc gacaaggcgg accggggcgg ggacggatag cggccgaaaa aaatttgccg
     2701 aacaatttct ctttttacag aaataacaaa cgaacagaaa agtggaggcg gagatgggca
     2761 gagtggcaca gcagaggatc cttccggtgg cggcaacgcc cgtaccggcg gaactggccg
     2821 acctgcgctt ttccgcgctg ctgggtgcgg ccggctgggc ccggctgccc gcggcggtgc
     2881 gggcccgctt tgcccaccgc tgcgcccccg gcgtctcggt cagctatgcc ggccatgtcg
     2941 ccgaatgccg gatgagcacg gcgggctggc tgctggccca ggcctgccgg ctgatcggcg
     3001 cgccgcttcc gctcgatcgc ggcggcgggc tggcggcggt ggtgacggtg accgaacacg
     3061 cccccagcgg cggccagacc tggacccgga tctacgcccg ccccggcggc tttccgcaag
     3121 tgatccattc ggccaagcgc tttgccgggc cgacaggcct cgaggaatac ctcggcctcg
     3181 gctttggcat cgccctgcgg ctggaggcgg gggaggaccg gatcggcttc ctctccgacc
     3241 actatttcct gcggctcggg ccggtgcggc
//
LOCUS       DASUDV010000103         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1151873,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000103 DASUDV010000000
VERSION     DASUDV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151873"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFV30_02250"
     CDS             <1..325
                     /locus_tag="VFV30_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4846939.1"
                     /translation="HLMKVAQQLAALGYVEARRGRGGGLVLAKPASAINLGAVVRALE
                     PTGQFVECFDRQAGACAIAGACGLQGALSLALGDFLARLDRYSLADLVPDPARFLKAL
                     ETAAA"
     gene            444..1208
                     /locus_tag="VFV30_02255"
     CDS             444..1208
                     /locus_tag="VFV30_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4846940.1"
                     /translation="MDLAGFDLVALLPFIAVGFAAQLVDGALGMAFGVISNTLLVGVL
                     GVPPALASQRVHVVECFTTATSGISHLIHGNIDKGLFFRLLVPGVIGGVLGAYLLTSL
                     DASVVKPFVLLYLTSIGLYILARGLFYPPKIREARLVWPLGLFGGFLDAAGGGGWGPV
                     VTSNLLVQGADPRKVVGTVNSVEFFLTLTISATFIWHLGVADLAGATLGLLIGGVMAA
                     PIGAWAAKHFNPKTMLILVGMVLTATSAYGVWKAWG"
     gene            1258..2061
                     /locus_tag="VFV30_02260"
     CDS             1258..2061
                     /locus_tag="VFV30_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase Amj family protein"
                     /protein_id="HEX4846941.1"
                     /translation="MDGPLLTICLLTASINLIGSLAYAARIAGVRTGRIAISFALFNI
                     LVLVSRLSNSFLGPFLAKRIETRMLAGSGQGLESDFRIILASASVAVAVGIVLVPSAQ
                     RLFARAIDWFQRNRSTARLVLHAATPSGLSVIRQSLTMPSRANLASLKRPQAVGWSVL
                     LANMIAQALLTVGVLASLYAGYLSPDFRVTASQLSAVVNGFATILLFALIDPQLSVMT
                     DDVVEGRLGAAQFRSAIVWISFSRLAGTVLAQALFLPSAYAIAWIANLV"
     gene            complement(2068..>2844)
                     /locus_tag="VFV30_02265"
     CDS             complement(2068..>2844)
                     /locus_tag="VFV30_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX4846942.1"
                     /translation="FEIENSAVSGHVPAMVELLYERLQRRGFLERDVRRMVNQDRNVF
                     ASLLLKLGVGDAMVTGMTRTFAQTMREVRQVLDPKESQLPFGIHLMIGKNYTVFLADT
                     TVNERPEAEDLARIAVETAAVAQRLGHTPRVAFLSYSTFGNPPGRWLEPLREAVAILD
                     RANPGFEYEGELAPDAALNPTVMKNYPFSRLSGPANVLIMPGLQSANISAKLLRELAG
                     NATIGPMLIGMEKPVQIIPMTAIAPDVLTLAVLAAAGVAG"
BASE COUNT          397 a          918 c          953 g          576 t
ORIGIN      
        1 tcacctgatg aaggtggccc agcagctcgc cgcgcttggc tatgtcgagg cgcggcgcgg
       61 gcgcggcggc ggactggtgc tggccaagcc ggccagtgcg atcaatctgg gcgcggtcgt
      121 ccgcgcgctg gagccgaccg gacagttcgt cgaatgcttc gatcggcagg ccggtgcctg
      181 cgcaatcgcc ggggcctgcg ggctgcaagg cgcgctcagc ctggcgctcg gcgatttcct
      241 ggcccgactc gatcgctaca gccttgccga tctggttccc gatccggcgc gcttcctcaa
      301 ggcactggaa accgccgcag cgtgaacgcc cgcgtcctgc cgcaggaaaa tttcgcttcg
      361 gcgccaaggt gaaaatcttt ctcggtcttg cgcacggtta actctatcac tccggtcgac
      421 attgcttctt ccggagtcga ccattggacc ttgccggttt cgatcttgtc gccctgctgc
      481 ctttcatcgc ggtcggcttt gccgcgcagc tggttgatgg cgcgctgggc atggccttcg
      541 gggtgatctc gaacaccctg ctggtcgggg tgctgggggt gccgcccgcg ctggcttcgc
      601 agcgggttca cgtggtcgaa tgcttcacta ccgcgacatc ggggatcagc cacctgatcc
      661 atggcaacat cgacaagggc ctgttttttc gcctgctggt tcccggcgtg atcgggggcg
      721 tgctgggggc ctatctgctg acgtcgctcg atgcttcggt ggtcaagccc ttcgtgctgc
      781 tctacctgac ctcgatcggg ctctatatcc tggcccgggg cctgttctat ccgcccaaga
      841 tcagggaggc ccggctggtc tggccactcg ggctgttcgg gggctttctc gatgcggccg
      901 gcggcggcgg ctgggggccg gtggtaactt cgaacctgct ggtccagggc gccgatccgc
      961 gcaaggtggt gggcactgtc aattcggtcg aattcttcct gaccctgacg atctccgcca
     1021 ccttcatctg gcaccttggc gtggcggacc tcgccggggc aacgcttggc ctgctgatcg
     1081 gcggggtcat ggctgcgccg atcggggcct gggcggcgaa gcacttcaac ccgaagacca
     1141 tgctgatcct ggtcggcatg gtgctgaccg caaccagcgc atacggggtg tggaaggcct
     1201 gggggtagtc cccgcccggc cctttcacgc cgctggatcg catgccatga agggccgatg
     1261 gacgggccgc tgctgaccat ctgcctgctg actgcgagca tcaacctgat cgggtcgctg
     1321 gcctatgccg cgcggattgc cggggtgcgc accgggcgga tcgcgatcag ctttgccctg
     1381 ttcaacatcc tggttctggt cagccggctg tcgaacagct tcctggggcc gttcctggcc
     1441 aagcggatcg aaacgcggat gctggccggg agcggccagg ggctggaaag cgatttccgg
     1501 atcatccttg caagcgccag tgtggcggtg gcggtaggca tcgttctggt gccgtcggcc
     1561 cagcggctgt tcgcccgcgc gatcgactgg ttccagcgca accgttcgac cgcgcggttg
     1621 gtgctgcacg cggcaacgcc atcgggtctc agcgtgatcc gccaatcgct gaccatgcct
     1681 tcgcgggcca accttgccag tctcaagcgc ccgcaagcgg ttggctggtc ggtgctgctg
     1741 gccaacatga tagcccaggc gctgcttacg gtgggcgtgc tggcttcgct ttatgccggt
     1801 tatctcagtc ccgatttccg ggtcaccgca tcgcagcttt cggccgtggt caacggcttt
     1861 gccacgatcc tgctgttcgc gctgatcgat ccgcagctct cggtcatgac cgacgatgtg
     1921 gttgaagggc ggcttggcgc cgcccagttc cgctcggcga tcgtctggat ttcgttcagc
     1981 cgcctggccg ggacggtgct ggcccaggcg ctgttcctgc cctcggccta tgccatcgcc
     2041 tggattgcca acctggtctg agccggttca gcccgccact cccgccgccg ccagcactgc
     2101 cagggtcagc acgtcgggag cgatcgcggt catcggaatg atctgcaccg gcttttccat
     2161 cccgatcagc atcggcccga tcgtggcgtt gccggccagt tcgcgcagca atttggccga
     2221 gatgttggcc gattgcagcc cgggcatgat cagcacgttg gccggccccg aaagcctgga
     2281 gaacggatag ttcttcatca ccgtggggtt gagcgcggca tcgggcgcca gttcgccttc
     2341 gtattcgaac ccggggttgg cccggtcgag gatcgccacg gcttcgcgca gcggctccag
     2401 ccagcggccc ggcgggttgc cgaaagtcga ataggacagg aatgcgaccc tgggggtatg
     2461 ccccaggcgc tgcgccacgg ccgcggtttc caccgcaatc cgggccaggt cttcggcttc
     2521 ggggcgttcg ttgacggtcg tgtcggccag gaacacggtg taattcttgc cgatcatcag
     2581 gtggatgccg aagggcagct ggctttcctt gggatcgagc acctggcgca cttcgcgcat
     2641 ggtctgggca aaggtgcggg tcatgccggt gaccatcgca tcgcccaccc ccagcttgag
     2701 cagcagcgag gcgaagacgt tgcggtcctg gttgaccatc cggcgcacgt cgcgttccag
     2761 gaacccgcgg cgctgcagcc gttcgtaaag cagctcgacc atggcgggaa cgtggccgct
     2821 gaccgccgaa ttctcgatct cgaa
//
LOCUS       DASUDV010000104         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1306540,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000104 DASUDV010000000
VERSION     DASUDV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306540"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..429
                     /locus_tag="VFV30_02270"
     CDS             49..429
                     /locus_tag="VFV30_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX4846943.1"
                     /translation="MADEQPPLVAEDAAERSLIDDVRQLAEDGRTLLEAELAYQKSRA
                     LVAGQAARGVAGWGALALALVFFALMALVLGLVLALTPLLGAFGATGAVFAGLVVMAA
                     LCGWIAARRWKRAAVQLGNEDAAR"
     gene            426..770
                     /locus_tag="VFV30_02275"
     CDS             426..770
                     /locus_tag="VFV30_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846944.1"
                     /translation="MNEAESRLAEDRQTRRSARGLFDTRLAQVKADLAARGVPARIKA
                     KAQDETFKAIDKGIDIAKESKGVIAATAGALLVWAFRAPLLKAAKGLFGQGSVQDQDL
                     SDDGNANEEQQA"
     gene            767..1249
                     /locus_tag="VFV30_02280"
     CDS             767..1249
                     /locus_tag="VFV30_02280"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846945.1"
                     /translation="MSEADDKRERIRSRIAESQERLTRESDQLPALPPRRAPADAYPP
                     EDLRSLASEHPWLVVAAGAGAGLLLGALLPKRAGSKLSGRALGLAAAGAELALALSRN
                     AREAAEDGAREGLQRLDERTAPLRRRAGEAAGTASRSARSTGVRIAGEAIKLATRLRK
                     "
     gene            1336..1563
                     /locus_tag="VFV30_02285"
     CDS             1336..1563
                     /locus_tag="VFV30_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX4846946.1"
                     /translation="MSEQKLHLVIGGRVKDPQTLEFVDLEAMDYVGVFPDYTSAEEAW
                     RSAARRTVDDAEMKYVVVHLHRLLEPDMLKR"
     gene            complement(1618..2094)
                     /gene="greA"
                     /locus_tag="VFV30_02290"
     CDS             complement(1618..2094)
                     /gene="greA"
                     /locus_tag="VFV30_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX4846947.1"
                     /translation="MASVEKLPMLAEGYERLVAELKALREERPKIVDAIEEARAHGDL
                     SENAEYHAAKERQGQVEMSIADLEDKVSRAQIVDPTTLSGDRIIFGATVTLLDDDDKP
                     VKYQIVGPYEADAKVGRISYNSPLGRALIGRRVEDEIEVTVPAGDRSYLVQKIEFI"
     gene            complement(2197..>2412)
                     /locus_tag="VFV30_02295"
     CDS             complement(2197..>2412)
                     /locus_tag="VFV30_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846948.1"
                     /translation="DRIIDGDVTLIFNTTEGWQSLKDSQSIRASALTGKVSYYTTAAA
                     SVAAAEAIAAMQNTSLEVRSLQDYYSH"
BASE COUNT          392 a          822 c          806 g          392 t
ORIGIN      
        1 ctgttcaagg gcggttcgaa cgaagcctga tcgccgaccg tccgggccat ggccgatgaa
       61 cagcccccgc tggttgccga ggatgcggcc gaacgctcgc tgatcgacga cgtgcgccag
      121 ctggccgagg acgggcgaac cctgctcgag gccgagctcg cctaccagaa atcgcgcgcc
      181 ctggtcgccg gacaggcggc caggggcgtg gctggctggg gcgccctggc gctggcgctg
      241 gtgttcttcg cgctcatggc gctggtcctc ggcctggtgc tggcgctgac cccgctgctc
      301 ggcgctttcg gcgcgaccgg ggcggtcttt gccggactgg tggtgatggc ggcgctgtgc
      361 ggctggatcg ccgcgcggcg ctggaagcgc gccgccgtgc agctgggcaa cgaggacgcc
      421 gcccgatgaa cgaggccgag agccgcctgg ccgaagaccg ccagacccgc cgctccgccc
      481 gcgggctgtt tgacacgcgt ctcgcccagg tgaaggccga ccttgccgcg cgcggggtgc
      541 cggcccggat caaggccaag gcccaggacg agaccttcaa ggcgatcgac aagggcatcg
      601 atattgccaa ggaaagcaag ggcgtgattg ccgccacggc cggggccctg ctggtctggg
      661 cctttcgcgc cccgctgttg aaggcggcca agggcttgtt cgggcaaggc tccgttcagg
      721 atcaggacct tagtgatgat ggcaatgcca acgaggagca acaggcgtga gcgaagccga
      781 cgacaagcgt gagcggatcc ggtcccggat tgcggaatcg caggagcgcc taacccgcga
      841 gagcgatcaa ctgcccgccc tgcccccgcg ccgcgcgccg gccgatgcct atccgcccga
      901 agacctgcgc agcctggcca gcgaacaccc ctggctggtc gtggccgccg gggccggagc
      961 cggcctgctg ctgggcgcgc tgctgcccaa gcgggccgga tcgaagctca gcggccgtgc
     1021 cctgggcctg gccgcggccg gggccgaact cgccctggcg ctcagccgca atgcccgcga
     1081 agccgccgag gacggcgcgc gcgagggcct gcagcggctg gacgagcgga ccgccccgct
     1141 gcgccgccgc gcaggagaag ccgccggcac cgcctcgcgc agcgcccggt cgaccggggt
     1201 gcggatcgcc ggggaagcga tcaagctcgc cacgcgcctg cgcaagtagg acctgccctt
     1261 aataccttgg tggtacttgc cttaaggcag gggcgcgact atgcgcgcgc tttctcctcc
     1321 ctcccaggta gaaccatgag cgaacagaaa ctccacctcg ttatcggcgg gcgcgtcaag
     1381 gacccgcaga cccttgaatt cgtcgatctg gaagcgatgg actatgtcgg ggtcttcccg
     1441 gactacacca gcgccgaaga ggcctggcgc agcgccgcgc gccgcacggt cgacgatgcc
     1501 gaaatgaaat acgtggtggt gcacctgcac cgcctgctgg aaccggacat gctcaagcgc
     1561 tgagccgccg ccagcccgga aagagaaagg ccccagaaat ccggggcctt tttcgtatca
     1621 gatgaactcg atcttctgga ccaggtagga ccggtcaccg gccggaaccg tcacctcgat
     1681 ctcgtcctcc acccggcgcc cgatcagtgc gcggcccagc ggggaattgt agctgatccg
     1741 gcccaccttg gcatcggcct cataggggcc gacgatctgg tacttcaccg gcttgtcatc
     1801 gtcatccagc aaggtgacgg tcgcaccgaa gatgatccgg tcgcccgaaa gcgtggtggg
     1861 atcgacgatc tgcgcgcggc tgaccttgtc ctccagatcc gcgatcgaca tctcgacctg
     1921 gccctggcgc tccttggcgg cgtggtattc ggcgttttcg gaaaggtcgc cgtgggcgcg
     1981 cgcttcctca atcgcgtcga cgatcttcgg ccgctcctca cgcagcgctt tcagctcggc
     2041 aacaaggcgc tcatagcctt ccgccagcat cggcagcttt tcgacacttg ccatcgttcc
     2101 tcatcctcct cacgcacttt cgcagcgact atgaacctgc cccgctcctt gccggatgcg
     2161 ggctctcagt cgccgatttg gggaaagcgc gattgatcag tgactataat agtcctggag
     2221 tgagcgcact tcaaggctcg tattttgcat tgcagcaatc gcctccgccg ccgcgaccga
     2281 ggccgccgcg gtggtgtagt acgaaacctt gccggtcagc gccgaagcgc ggatcgattg
     2341 cgaatccttc aggctctgcc agccctcggt ggtgttgaag atcagcgtca cgtcgccgtc
     2401 gatgatccgg tc
//
LOCUS       DASUDV010000105         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1770596,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000105 DASUDV010000000
VERSION     DASUDV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770596"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..406)
                     /gene="fliN"
                     /locus_tag="VFV30_02300"
     CDS             complement(131..406)
                     /gene="fliN"
                     /locus_tag="VFV30_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX4846949.1"
                     /translation="MTLHTRGFDLIKEVDVRLSVELGRTEMKLRDVLALGEESVVMLD
                     RLTDELLDVMVNGKLIARGEIVAQGGRFGLRIVELAGAQPETQAGAA"
     gene            complement(419..1303)
                     /locus_tag="VFV30_02305"
     CDS             complement(419..1303)
                     /locus_tag="VFV30_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX4846950.1"
                     /translation="MKPERAFTAERIAAQHCPELLQRAAGCEPADLLGDFTRLGDRLA
                     RQLAPELARLLGGEEPVVHVSAARELSEGELASELGQLAANSLIASGVPGIALLASIE
                     GSGLLRLVDRAFGGKGDAPAVMPKVFPLSANLMIDRIEALLAAALGGSLGQEGLRVLR
                     RDTSLAELAPFPAGARLALLSCEVAEGARAPWKFAIALPLVSLPRLFGNAAATPREPR
                     SADPASEPFATMPLTLVATLVDMAMPISRLSTLEPGTVLPVAVARSVPITLGSATLAR
                     GTVGAQDDRIAIKLTQIA"
     gene            complement(1300..1893)
                     /locus_tag="VFV30_02310"
     CDS             complement(1300..1893)
                     /locus_tag="VFV30_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX4846951.1"
                     /translation="MSDSNEAEAKPRKKKGKGFLIKGLVGLILIAAGGGGTFALVQAG
                     MLGDHAEEKKEADHPKLVKKGEEDPYAPKTEAKEGEGLPEVEGEGGSEYRTSYYNFTE
                     DFTSNLRNSSALVQISLACSTRRDGRVLMWLKKHELAIRSEILVVLADTPEEDAFTPD
                     GKQRLQKRMTDAINKVLTKTEGFGGVDQVYFKSYLVQ"
     gene            2031..>2303
                     /locus_tag="VFV30_02315"
     CDS             2031..>2303
                     /locus_tag="VFV30_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846952.1"
                     /translation="MCRTSPGSGIAICALLGPGGSLAWRFCGESLQDWRRRRAGDPGK
                     VGIGACQRNAKRLAIVVQAQADFAEIGMLNRKGDRLRLNGIARSNQP"
BASE COUNT          367 a          755 c          751 g          430 t
ORIGIN      
        1 atccgcaccg aacgcgcccc cgattggttg ccagcgatcc tggcctgcag gcgctgcgcc
       61 agcttcagcg tgccccatgc tatcgcagcg atcagcggca gcaggacgat cagcttgatc
      121 agataccaga tcatgccgca cctgcctgcg tttcgggctg ggcgccggcc agttcgacaa
      181 tccgcagtcc gaaccgtccc ccttgggcca cgatctcgcc gcgcgcgatc agcttgccgt
      241 tgaccatcac gtcgagcagc tcgtcggtca gccgatcgag catgaccaca ctttcctcgc
      301 ccagtgccag gacgtcgcgc aatttcatct cggtgcggcc cagttcgacc gaaaggcgga
      361 catcgacttc cttgatcagg tcgaagccgc gggtgtgcaa tgtcatcgtc gtgttccttc
      421 aggcaatctg ggtcagcttg atcgcgatgc gatcgtcctg tgcgccaacg gtgccgcgcg
      481 cgagcgtggc ggaacccagc gtgatcggca cggagcgggc aaccgccacg ggcagcactg
      541 ttccgggttc aagcgtcgac aagcgcgaaa tcggcatcgc catgtcgacc agggtggcga
      601 ccagggtcag cggcatggtc gcaaacggtt ccgaagccgg atcggcagag cgtggctccc
      661 ttggcgtggc ggcggcattg ccgaacaggc ggggcagcga aaccagcggc agcgcaatgg
      721 caaacttcca cggtgcacgg gccccttcgg ccacttcgca agagagcagc gccagccggg
      781 caccggcagg aaatggggcc agttcggcaa ggctcgtgtc gcggcgcaat acacgcagtc
      841 cctcctggcc aagcgaaccg ccaagcgcgg ctgcgagcag cgcctcgatc cggtcgatca
      901 tcagattggc cgaaagcgga aagaccttgg gcatcacagc cggggcatcg cccttgccgc
      961 caaaggcccg gtcgaccagc cgcagcaggc ccgaaccttc gatcgaggcg agtagcgcga
     1021 tgcccggcac cccgctggcg atgaggctgt tggcggccag ttggccaagc tcgctggcca
     1081 attcgccttc ggacaattcg cgcgccgcgc tgacgtggac gaccggttcc tcgccgccca
     1141 gcagccgcgc cagttcgggc gcaagctggc gggccagccg atcgcccagc cgggtgaagt
     1201 cgcccagcag gtcggctggt tcgcagccgg ccgcgcgttg cagcagttcg gggcaatgct
     1261 gcgcggcgat ccgttcggca gtgaaggcgc gttcgggctt cactggacga ggtagctctt
     1321 gaagtagacc tgatcgaccc cgccgaagcc ctcggtcttg gtcagcacct tgttgatcgc
     1381 atcggtcatg cgcttttgca ggcgctgttt gccgtcgggg gtaaaggcat cctcctcggg
     1441 cgtgtcggcg aggaccacca ggatctcgct gcggatcgcc agttcgtgct ttttcagcca
     1501 catcagcacc cggccatcgc gccgggttga acaggccagg ctgatctgga cgagagcgct
     1561 cgaattcctg aggttcgagg taaagtcttc ggtgaagttg taatagctgg tgcggtattc
     1621 gctgccgcct tcgccctcga cttcggggag accttcgccc tccttggctt cggtcttggg
     1681 ggcataggga tcttcctcgc ctttcttgac cagcttggga tgatcggctt ccttcttttc
     1741 ctcggcatgg tcgccgagca tcccggcctg gaccagcgcg aaggtgccgc cgccccccgc
     1801 ggcgatcagg atcaggccga ccaggccctt gatcaggaag cccttgccct tcttcttcct
     1861 gggcttggct tcggcttcgt tgctgtcact catggctatt tacccccgga ttggtgtgct
     1921 gttcaggcaa agatgccgcc gcgtggattg ccccgctcgt gggcttgatg ttcggtcctg
     1981 ggctgctggc gtggctgatc ctcgcggcgt tcaggcgatc cgccgcgtga ttgtgccgca
     2041 cttcccccgg ttccggcatt gccatttgcg ctctgctcgg gccgggcgga tccctggcct
     2101 ggcgtttctg cggcgagagc ttgcaggact ggcggcggcg ccgtgctggc gatccgggca
     2161 aagtcgggat cggcgcttgc cagcgaaatg ctaagcgcct cgccatcgtg gtgcaggcgc
     2221 aggctgattt cgccgaaatc ggcatgctga accgcaaagg agaccgcctg cggctgaacg
     2281 gcatcgcgcg cagcaaccag ccg
//
LOCUS       DASUDV010000106         4938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_776411,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000106 DASUDV010000000
VERSION     DASUDV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776411"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFV30_02320"
     CDS             <1..756
                     /locus_tag="VFV30_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX4846953.1"
                     /translation="LAIQKMRSSGADRVTEPLPLEVQPLVVELNALLAHTERQAEEAR
                     THAGNLAHALKTPLTVVMNAATAKAPDLADTVIREAAVMRRQVDHHLARARAVGRRAV
                     GQARANVGESVEAVLRAVTVLYEKTRFDLDGKRDALVAIERQDLDEILGNLIENAAKY
                     GGGSVFVTIDPAGEADWCEIWVEDDGLGIPAEERVRIFDRGARLDTGKPGTGLGLAIV
                     RDVAEIYGGSVTLDESEDLGGLLVKLRLPRARG"
     gene            762..1451
                     /locus_tag="VFV30_02325"
     CDS             762..1451
                     /locus_tag="VFV30_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846954.1"
                     /translation="MNPGKALLAAAALLPLSACNFVVSEQPWFTAASGPQLKDGLWAN
                     LDSPNCAFDPASPITDWPKCASPMMIRGSVYSGPPSGEAAEAPGALTDASKWQPIEHV
                     LVEGDPQIDQIAIPLDMTKQAPGAAGREMKPIHLYLAVRPTAKDAEGRITEAQRWPVL
                     CGPMPKKPREKNGVPIFTSDRPYKGIRVKDEFCMAESAAALRAAAVSSEVAAKEGGFT
                     IVTSRWIRDGL"
     gene            1474..2136
                     /locus_tag="VFV30_02330"
     CDS             1474..2136
                     /locus_tag="VFV30_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846955.1"
                     /translation="MGRLLIAGAVLALGQAAPAFAGDGFCRNGIFTEQNPAFGLARIS
                     GQGKARFYDDMNGCPSVGPSCQQKTYVVPGDRVVTGRSLGQFVCVYFPNGVGGTAGWM
                     PRSRLTALPVNPRPPLTAWLGRWADNDNWVRFSRAGSGLKAEGMAYWPGANPSPEDRP
                     GGPNTGEIAGLLRLSGNRAVEPECQVTFHLLNDILIAVDPVRRCDGMNVTFSGVYQRV
                     KR"
     gene            complement(2146..2442)
                     /locus_tag="VFV30_02335"
     CDS             complement(2146..2442)
                     /locus_tag="VFV30_02335"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX4846956.1"
                     /translation="MSDPAPDPVLASFRSSIDNIDAALIHILAERFRITKAVGAYKAE
                     KALPASDPGREEQQIARLRKLAEDAQLDPEFSEKFLRFIIQEVIRHHEQAAEAK"
     gene            2530..3558
                     /locus_tag="VFV30_02340"
     CDS             2530..3558
                     /locus_tag="VFV30_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX4846957.1"
                     /translation="MSADVIPLRPNAGGPDSEPSLGPIMALTANGMNAVNQVILERMQ
                     SEIPLIPALAGHLIAGGGKRMRPMLTLAGAALCDYHGTRHHKLAAAVEFIHTATLLHD
                     DVVDGSEMRRGKAAANLIFGNPATVLVGDFLFTRSFELMVEDGSLKVLKILSNASSVI
                     SEGEVDQLTAARQIETSEERYLSIIGSKTAALFAAATRIAAVVAERPEAEERALEDYG
                     RNLGIAFQLVDDAMDYDSEASEMGKGKGDDFREGKMTLPVILAYARGSAEEREFWKAA
                     IQGHRISDADLAHAVELIRRHDAVSETRERARHFAQRAINAIAIFPQGEARAAMTEAA
                     EFAVARRS"
     gene            3843..4166
                     /locus_tag="VFV30_02345"
     CDS             3843..4166
                     /locus_tag="VFV30_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX4846958.1"
                     /translation="MIEYHNSRPLRIVGPLREVLTRDNLPPRNTTRWVASRKAQVVAA
                     VESGLITIEEAMARYSLSLEEFCSWQRAMDRAGVSGLRVGWSKDDRAGRRRRASPRAL
                     SLVTH"
     gene            4302..>4938
                     /locus_tag="VFV30_02350"
     CDS             4302..>4938
                     /locus_tag="VFV30_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4846959.1"
                     /translation="MQRRRLGKSAIVVSDICMGTMTFGSQTDEAEALRILDQCFEAGI
                     DFYDTAEGYPVPPDPKWVGRTEEIVGKWLKTKPRDAIILATKVSGPSHVWFRSPKREG
                     MTALDRRNIMVAVEDSLKKLGTDYIDLYQTHWPDHELPYDETMEVLDELVRDGKVRIV
                     GCSNETSWGLMKSIQTAERLGTVRYQTIQNNFSLNNRRFEDELAQVCRMEGV"
BASE COUNT          850 a         1635 c         1691 g          762 t
ORIGIN      
        1 ctggcgatcc agaagatgcg cagttccggc gcggaccggg tgaccgagcc cctgccgctg
       61 gaggtccagc cgctggtggt cgagctcaac gcgctgctgg cccataccga gcgccaggcc
      121 gaagaggcgc ggacccacgc cgggaacctg gcccatgcgc tgaaaacccc gctgaccgtg
      181 gtgatgaacg ccgcgaccgc caaggcaccc gacctggccg acaccgtgat ccgcgaggcg
      241 gcggtgatgc gccggcaggt cgatcaccac ctcgcccggg cccgcgcggt cggccgccgg
      301 gcggtggggc aggcgcgcgc caacgtgggc gagagcgtgg aagcggtgct gcgcgcggtg
      361 accgttctct acgaaaagac ccggttcgat ctggacggca agcgcgacgc gctggtcgcg
      421 atcgaacggc aggacctcga cgagatcctg ggcaacctga tcgagaacgc cgccaagtac
      481 ggcggcggat cggtcttcgt cacgatcgat ccggccggcg aggccgattg gtgcgagatc
      541 tgggtcgagg acgatggcct gggcattccg gccgaagagc gggtccgcat cttcgatcgc
      601 ggcgcacggc tggacaccgg caagcccggc accggcctgg gcctggcaat tgtccgtgac
      661 gtggcggaaa tctatggcgg gtcggtgacc ctggatgaaa gcgaagacct gggcggcctg
      721 ctggtcaaac tgcgcctgcc ccgagcgaga ggatgaaccc gatgaaccct ggaaaagccc
      781 tgcttgcggc cgcagccctg ctgcccttgt cggcctgcaa ctttgtcgtg tcggaacagc
      841 cgtggttcac cgcggcgtcc gggccgcagc tcaaggacgg gctgtgggcc aacctcgaca
      901 gcccgaactg cgctttcgat ccggccagtc cgatcaccga ttggcccaag tgcgcctcgc
      961 cgatgatgat ccgcggatcg gtctattccg gcccgccctc gggcgaagcc gccgaagcgc
     1021 cgggcgcgct gaccgatgcc tccaaatggc agccgatcga gcacgtgctg gtcgaaggcg
     1081 atccgcagat cgatcagatc gcgatcccgc tcgacatgac caagcaggcc ccgggcgcgg
     1141 ccgggcgcga aatgaagccg atccatcttt acctggccgt ccggccgacc gccaaggacg
     1201 cggagggccg gattaccgag gcccagcgct ggccggtgct gtgcggcccg atgcccaaga
     1261 agccgcgcga gaagaacggc gtgccgatct tcacctccga ccggccctac aaggggatcc
     1321 gggtgaaaga cgagttctgc atggccgaaa gcgcggcggc gctgcgggcc gcggccgtct
     1381 ccagcgaggt tgcggccaag gaaggcggat tcacgattgt gaccagccgc tggatccgcg
     1441 acgggctctg acggcgaaac ggaggagacg ggcatgggcc ggttgctgat tgcgggcgcg
     1501 gtgctggcgt tgggacaggc ggccccggcc tttgccggtg acgggttctg ccgcaacggg
     1561 atcttcaccg aacagaaccc cgcgttcggc ctcgcccgga tcagcgggca gggcaaggcg
     1621 cggttctatg atgacatgaa cggctgcccg tcggtgggcc cgtcgtgcca gcagaagacc
     1681 tatgtcgtcc cgggcgaccg ggtggtgacc gggcgcagcc tcggccaatt tgtctgcgtc
     1741 tatttcccca acggggtcgg cgggacggcc gggtggatgc cgcgcagccg gttaacggcg
     1801 ctgccggtga acccgcgtcc gccgctgacg gcctggctgg gccgctgggc cgataacgac
     1861 aactgggtcc gcttctcccg cgccggcagc gggctcaagg cagaaggcat ggcctactgg
     1921 ccgggcgcca acccctcgcc ggaagaccgg ccaggcggcc ccaacaccgg cgagattgcc
     1981 ggcctgctgc gcctcagcgg caaccgcgcg gtggagccgg agtgccaggt gaccttccac
     2041 ttgctgaacg acatcctgat cgccgtcgat cccgtgcgcc gctgcgacgg gatgaacgtg
     2101 accttctcgg gggtttacca gcgggtgaag cgctagcctc cgaggctact tggcttcggc
     2161 cgcctgttcg tggtggcgga tcacttcctg gatgatgaag cgcaggaact tttccgagaa
     2221 ctcgggatcg agctgcgcgt cctcggccag cttgcgcaac cgggcgatct gctgctcctc
     2281 gcggccgggg tcgctggccg gcagggcctt ctccgccttg taggcgccaa ccgccttggt
     2341 gatgcggaac cgttcggcca ggatgtggat cagcgccgca tcgatgttgt cgatcgagga
     2401 gcggaagctg gcaaggacgg gatcgggtgc gggatcggac atgtttgacc cgctagcatg
     2461 acattttgcg caagaccatt gcctgctttg cacttgccaa gccggtttcg cggcttcaag
     2521 ggcggggcga tgagcgcaga tgtgatccct cttcgcccca atgccggtgg acccgacagc
     2581 gaaccgtcgc tggggccgat catggcgctg accgccaacg gcatgaacgc agtcaaccag
     2641 gtgatcctgg agcggatgca gagcgagatc ccgctcatcc cggcgctggc cggccacctg
     2701 attgccggtg ggggcaagcg gatgcggccg atgctgacgc tggccggggc ggcgctgtgc
     2761 gattaccatg gcacccgcca tcacaagctg gccgccgcgg tggaattcat ccacaccgcc
     2821 accctgctgc acgacgatgt ggtcgacggc agcgagatgc gccgcggcaa ggccgccgcc
     2881 aacctgatct tcggcaatcc agccacggtg ctggtcggcg atttcctgtt cacccgcagc
     2941 tttgagctga tggtcgagga cggcagcctg aaagtgctca agatcctctc caacgccagt
     3001 tcggtgatca gcgaagggga ggtcgatcag ttgaccgccg cccgccagat cgaaaccagc
     3061 gaggaacgct atctctcgat catcggatcg aagaccgcgg cgctgtttgc cgccgcaacc
     3121 cggatcgccg cggtcgtcgc cgaacggccc gaagccgaag agcgcgcgct ggaggattac
     3181 ggccgcaacc tgggcatcgc cttccagctg gtcgacgatg cgatggatta cgattccgaa
     3241 gcctcggaaa tgggcaaggg caagggcgat gatttccgcg agggcaagat gaccctgccg
     3301 gtgatcctgg cctatgcccg cggcagcgcc gaggagcgcg agttctggaa ggccgcgatt
     3361 caggggcacc ggatctccga tgccgatctg gcccacgcgg tggagctgat ccggcggcac
     3421 gacgcggtca gcgaaacgcg cgagcgggcc cgccatttcg cccagcgcgc gatcaacgcg
     3481 atcgcgatct tcccgcaggg cgaggcccgc gcggcgatga ccgaagcggc ggaattcgcc
     3541 gtcgcgcggc gcagctagac tgccggtttc ggccgctgcg ggccgctggc ccgtattgcc
     3601 tggctcttag aaggctgccc aggctggtgg cgccgagtcc gcttggcggc ttgcttgccc
     3661 gtaccggcca agcggcgcga acgcttcgtc gttcggtgcc accggcccgc cgtcacccgc
     3721 cctgatgctg cggtgagccg caggccggat tcaccccgat ttaaccacgc cggccggaga
     3781 ctgcttcgtg cttgggggcc caacctgacc tgtcaaaagg tcactgtcac aagggccgta
     3841 acatgatcga atatcacaat tcccgcccgc tgcggattgt cgggccgctg cgcgaagtgc
     3901 tgacgcgtga caacctgccg ccgcgcaata ccacccgctg ggtggccagc cgcaaggccc
     3961 aggtcgtggc cgcggtggag agcggtctga tcaccatcga agaggccatg gcgcgctaca
     4021 gcctcagcct ggaggagttc tgctcctggc agcgggcgat ggaccgcgcc ggggtttccg
     4081 ggctgcgggt cggctggtcc aaggatgacc gcgccggccg ccggcgccgc gcctcgccgc
     4141 gcgcgctttc gctggtcacg cactgacccg gcgggtcccg ccgcgccgcc gcttccgact
     4201 gcccccgagc ggagcggcgg cgcgcggggc ttgaccgccc ctctgcaaac agcttcactc
     4261 cggttgcgaa cgaaaaccaa tccgaccggg agaacacctg catgcaacgc cgccgtctgg
     4321 gcaaaagcgc catcgtcgtt tccgatatct gcatgggcac catgaccttt ggcagccaga
     4381 ccgacgaggc ggaggcgctg cgcattctcg accagtgttt cgaggccggg atcgatttct
     4441 atgacaccgc cgaaggctat ccggtgccgc ccgatcccaa atgggttggc cggaccgagg
     4501 aaatcgtcgg caagtggctc aagaccaagc cgcgcgacgc aatcatcctc gccaccaagg
     4561 tgtccggtcc cagccacgtc tggttccgca gccccaagcg cgaaggcatg accgcgcttg
     4621 accgccgcaa catcatggtc gcggtggaag acagcctgaa gaagctcggc accgattaca
     4681 tcgaccttta tcagacccac tggcccgatc acgagctgcc ctatgacgag accatggaag
     4741 tgctcgacga actggtccgt gacggcaagg tgcggatcgt cggctgctcg aacgaaacca
     4801 gctggggcct gatgaaatcg atccagaccg ccgagcggct gggcacggtg cgctaccaga
     4861 cgatccagaa caatttcagc ctcaacaacc gccggttcga ggacgagctg gcccaggtct
     4921 gccggatgga aggggtca
//
LOCUS       DASUDV010000107         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2013566,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000107 DASUDV010000000
VERSION     DASUDV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013566"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFV30_02355"
     CDS             complement(<1..211)
                     /locus_tag="VFV30_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX4846960.1"
                     /translation="MAEPAKPARPAGGIGAAVAAAERRAHRSLLRGHGGGHAPVSNLE
                     LFFDLVFVFAITQLSHFLGHHLDWLG"
     gene            complement(195..1103)
                     /locus_tag="VFV30_02360"
     CDS             complement(195..1103)
                     /locus_tag="VFV30_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4846961.1"
                     /translation="MSAEQTRIRLASGIELDVVDCGPRDAPALIFLHGFPESHRTWRH
                     QIAHLSDRYRCIAPDQRGYRGSSKPQEVEAYTPDKLIGDIFQLADALGIDRFTVLGHD
                     WGGAIAWGVALMGQMNGRVPRAVIANAPHPVGFSRLVWLNRHQRESSQYMRTFRDTAN
                     DGLVREHGLGGMLMKAFAGRENTEDKMEPAERAQLLQDWENREAAFGMLNWYRASPLY
                     VPAMDEPYAIPADRQPPPIPPLTIPTLVIWAMDDFALPPENLDGLDAMVPGMTLVKVP
                     GCGHFVPWEAPQAVNAALDEFLGRTG"
     gene            complement(1100..1921)
                     /locus_tag="VFV30_02365"
     CDS             complement(1100..1921)
                     /locus_tag="VFV30_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4846962.1"
                     /translation="MTTSTKAVWITGASSGIGAALARAYARRGCRLILSGRNEAALAE
                     VAAQCGVDHLILPFEVSDFAAGLAAADKAWEWSGGIDVLVNNAGISQRSLAIDTDFAV
                     YQKMIAIDLLAPINLTQALIKRMAARKTGQFIMISSLAGKIGSPLRSAYCAAKHGLVG
                     YADSIRLELNQHGISVIVATPGSIRTDVSRNAITSDGSTRGVSDEAIDNGVEPDDFAE
                     QMLAAIDAGEREIVVAKHPMEVMIGEARRTPEALFDKMIENFNAGYAAKMGVSEQ"
     gene            complement(2012..>2336)
                     /locus_tag="VFV30_02370"
     CDS             complement(2012..>2336)
                     /locus_tag="VFV30_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX4846963.1"
                     /translation="ADIELAPGGSIPIDPDASERAVMLVSGGASLDGQALRQFDLNLL
                     DAGGQLRLSSETGGRIMLLGGEPLYTPRYLWWNFVSSSQERIRAATRDWQQGRFPPVP
                     GDPDN"
BASE COUNT          399 a          767 c          763 g          407 t
ORIGIN      
        1 cgcccagcca atcgaggtgg tggcccagaa agtgcgacag ctgggtaatc gcgaagacga
       61 agaccagatc gaagaacagt tcgagattgc tgaccggggc atgcccgccg ccgtggccgc
      121 gcagcagcga gcggtgggca cggcgttccg ccgctgcaac cgcggcgcca attccgcctg
      181 ccgggcgggc gggcttagcc ggttcggcca aggaattcgt ccagcgcggc attgaccgcc
      241 tgcggtgcct cccagggcac gaagtggccg caaccgggaa ccttgaccag ggtcatgccc
      301 ggcaccatcg cgtccagccc atcgagattt tcgggcggca gcgcgaaatc gtccatcgcc
      361 cagatcacca gcgtgggtat ggtgagcggc gggatcggcg ggggctggcg atcggcgggt
      421 atcgcgtatg gctcgtccat cgcaggaacg tagagcgggc tcgcccggta ccagttgagc
      481 atgccgaagg cggcttcgcg gttttcccaa tcctgcagca gttgcgcccg ctcggccggt
      541 tccatcttgt cttcggtatt ttcccggccg gcaaaggcct tcatcagcat tccgcccagg
      601 ccgtgttcac gcaccagccc atcgtttgcc gtatcgcgga aggtgcgcat gtactggctc
      661 gattcgcgct ggtggcggtt gagccagacc agccgcgaga agcccaccgg atgcggggca
      721 ttggcgatca ccgcgcgtgg cacccgcccg ttcatctgcc ccatcagggc cacgccccag
      781 gcaatcgcgc cgccccagtc gtgaccgaga accgtgaagc gatcgatccc cagcgcatcg
      841 gccagctgga atatgtcgcc gatcagcttg tcgggggtgt aggcttcgac ctcttgaggt
      901 ttggacgagc cgcggtagcc gcgctgatcg ggggcgatgc agcgatagcg atcggaaagg
      961 tgggcgatct ggtggcgcca ggttcggtga ctttcaggaa agccgtgcag gaaaatcagc
     1021 gcgggggcat cgcgcgggcc acaatcgacc acatcgagtt cgatcccgct cgccaggcgg
     1081 atcctggtct gctccgcgct cactgctcgc tcacgcccat cttcgcggca tagccggcgt
     1141 tgaagttttc gatcatcttg tcgaacagcg cctcgggtgt gcggcgcgct tcgccgatca
     1201 tcacttccat cggatgcttg gccaccacga tctcgcgttc cccggcatcg atcgcggcga
     1261 gcatctgttc ggcgaaatca tccggttcca cgccgttgtc gatcgcttcg tcgcttaccc
     1321 cgcgggtcga gccatcggag gtgatggcat tgcgcgagac atcggtgcgg atcgatccag
     1381 gggtggcaac gatcaccgaa atgccgtgct ggttgagctc gagccggatc gaatccgcat
     1441 agccgaccag gccgtgcttg gccgcgcagt aggccgagcg cagtggcgag ccgatcttgc
     1501 cggcaaggct cgaaatcatg atgaactggc cggtcttgcg cgcggccatc cgcttgatca
     1561 gcgcctgcgt cagattgatc ggggcgagca gatcgatcgc gatcatcttc tggtacaccg
     1621 caaaatcggt atcgatcgcc aggctgcgct gactgatccc ggcattgttg accagaacgt
     1681 cgatcccgcc gctccactcc caggccttgt cggcggcggc aagccctgcg gcaaagtccg
     1741 atacctcgaa cggcaggatc aggtggtcga cgccgcattg cgcggcaacc tcggccagcg
     1801 cagcctcgtt gcggcccgaa aggatcagcc ggcatccgcg cctggcatag gcccgggcca
     1861 gggccgcgcc gatcccggat gatgcgccgg ttatccagac tgctttggtc gaagtggtca
     1921 tcccagcgct ccctgtcact cgattcggtt ctgccaagca gcgaagcaat cccgcgcccg
     1981 gcgcgcaacc ctcgattgcc gatcggcgcg atcagttgtc cggatcgccc ggcaccgggg
     2041 gaaagcggcc ctgctgccaa tcgcgcgtgg cggcgcggat ccgttcctgc gatgaggaga
     2101 cgaagttcca ccacagatag cgcggtgtat agagcggctc gccgcccagc agcatgatcc
     2161 gtccgcccgt ttcggaggac aggcgcagtt gcccgccggc atcaagcagg ttgagatcga
     2221 actggcgcag cgcctggcca tcaaggctgg ccccgccgct caccagcatc accgcgcgtt
     2281 cgctggcatc gggatcgatc gggatcgagc cacctggcgc cagctcaatg tcggcg
//
LOCUS       DASUDV010000108         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1792774,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000108 DASUDV010000000
VERSION     DASUDV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792774"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..786)
                     /locus_tag="VFV30_02375"
     CDS             complement(43..786)
                     /locus_tag="VFV30_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX4846964.1"
                     /translation="MPELKDRSSLALILGGLALLGLGAAGGWAFEKNRSSALNRAEVE
                     QVVREYILAHPEILPEAIERLRAKENSKQVTAAGDEVFVPFPGAVMGNPAGKTTLVMF
                     TDFACGYCRSSMKDVEALIAEDPDLKVVMRELPIIAPTSPNAARWGLAAAEQGRYPAF
                     HRAMFAAGRTDQASIDAAARAAGLDLERARRFAADPKVTAEIEANIAFARGLQLEGTP
                     AWVIGDQLMTGAVGKDLLAKAIAEEKAKS"
     gene            complement(796..2160)
                     /locus_tag="VFV30_02380"
     CDS             complement(796..2160)
                     /locus_tag="VFV30_02380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4846965.1"
                     /translation="MRTISRLLTRAVALAAALGLAAQPVAAQSILRDAETEALFRDMS
                     APLIKAAGLDPRNVDIVLIGDPSLNAFVAGGQAVYIHAGLIEAATSANEVQGVIAHEL
                     GHITGGHVISDGGTKAATGISLLSLVLGGLAAAAGAGDAAMGVFMAGQQAALGKYLAF
                     SRSQEASADAAGASYLSRAGITGRGSISFFKKLQNLEYRYGYQRNIDTEFYRTHPFTS
                     DRIATLQDTYEKDPAWNAPSDPELEARFKRVQAKLMGYVQEPKRTLQIYPETRMDVAA
                     HYARAYARHKEAYLDKAMAEADALLTTAPNDPYFLELKGQILLEAGRPREALAPLRRA
                     TELTGSQPLIATTFGHALLASEDPALLGEAERVLRAAVGRDRENPFAWYQLGIIHGQR
                     GDMARAKLASAEQQVLMLNFPQALKSAEEAEAALPKGSADWLRAQDIAMQSRAAIEQR
                     AKRR"
     gene            complement(2171..>2457)
                     /locus_tag="VFV30_02385"
     CDS             complement(2171..>2457)
                     /locus_tag="VFV30_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4846966.1"
                     /translation="TQPIHFVRADAPPMLLLTGDTDETVKPRNSRALARALDAVGRPV
                     EQVVLAGVDHAGPILKLAAPFNRDRRVLDPVLDFLARHGGASAPVQAARR"
BASE COUNT          390 a          805 c          833 g          429 t
ORIGIN      
        1 ggcagaacgg ccatgacttg cctgttctag cagaaaatca cgtcaggact tggctttttc
       61 ttcggcgatc gccttggcca gcagatcctt gccaaccgcg ccggtcatca gctgatcgcc
      121 aatcacccag gcgggtgtgc cttccagttg caggccgcgg gcaaaggcaa tgttcgcctc
      181 gatctcggcg gttaccttcg gatcggccgc gaaacgccgg gcacgctcga ggtcgagccc
      241 ggcggcgcgc gctgcggcat cgatgctggc ctgatcggtc cgtccggcgg cgaacatcgc
      301 ccggtggaag gcgggatagc ggccctgttc ggccgccgcc aggccccagc gcgcggcgtt
      361 ggggctggtc ggcgcgatga tcggcaattc gcgcatcacc accttgagat caggatcttc
      421 ggcgatcagc gcctcgacgt ccttcatcga gctgcggcag tacccacagg caaaatcggt
      481 gaacatcacc agcgtggtct tgcccgccgg attgcccatg accgcgccgg ggaacggcac
      541 gaacacttca tcgcccgcgg cggtaacctg cttcgaattt tccttcgcgc gcagccgttc
      601 gatcgcctcg ggcaggattt cgggatgcgc gaggatatat tcgcgcacca cctgttccac
      661 ctcggcgcgg ttgagcgcgc tgctgcggtt cttctcgaag gcccaaccac ccgccgctcc
      721 caggccaagc agggcaaggc cgccaaggat cagggcgagc gacgaacggt ctttgagttc
      781 aggcatgcgc gacagctacc ggcgtttggc gcgctgttca atcgccgcgc gcgattgcat
      841 cgcgatgtcc tgggcgcgca gccagtcggc cgagcccttg ggcagggcag cttcggcctc
      901 ctcggcgctc ttgagcgcct gggggaagtt cagcatcagc acctgctgtt cggcgctggc
      961 cagcttggcc cgggccatgt cgccgcgttg cccatggatg atgccaagct ggtaccaggc
     1021 gaaggggttt tcgcggtcgc gtcccactgc ggcgcgcagc acgcgctcgg cctcgcccag
     1081 cagtgcaggg tcctcgctgg ccagcagggc atggccgaag gtggtggcga taagcggctg
     1141 actgccggtc agttcggtgg cgcggcgcag cggcgccagc gcctccctgg gccgcccggc
     1201 ctcgagcaga atctggccct ttagctcgag gaaataggga tcgttggggg ctgtggtgag
     1261 caaggcatcg gcctcggcca tggccttgtc gagataggcc tccttgtggc gggcataggc
     1321 ccgggcataa tgcgcggcca cgtccatgcg ggtttcggga tagatctgca gcgtgcgctt
     1381 gggttcctgg acatagccca tcagcttggc ctgaacccgc ttgaagcgtg cttccagttc
     1441 cgggtcgctc ggcgcgttcc aggccggatc cttttcgtaa gtatcctgaa gggtcgcgat
     1501 gcggtccgag gtgaaggggt gggtgcggta gaattcggta tcgatattgc gctgatagcc
     1561 atagcgatat tcaaggttct gcagcttctt gaagaagctg atcgagccgc gcccggtgat
     1621 cccggcacgc gagagatagg atgcgcccgc ggcgtcggcc gatgcttcct gactgcgcga
     1681 aaaggccagg tacttgccca gcgccgcctg ctggccggcc atgaacacgc ccatcgccgc
     1741 atcgcccgcg ccggctgcgg cggccagtcc gcccagcacc aggctaagca gcgaaatccc
     1801 ggttgccgcc ttggtcccgc catcgctgat cacatggcca ccggtgatgt ggcccagttc
     1861 gtgggcgatc acgccctgca cctcgttggc gctggtcgcc gcctcgatca gcccggcatg
     1921 gatataaacc gcctggccac cggccacgaa ggcgttgagc gagggatccc cgatcagcac
     1981 gatatcgacg ttcctgggat caagcccggc ggccttgatc agcggcgcgg acatgtcgcg
     2041 gaacagggct tcggtttcgg catcgcgcag gatcgattgg gccgctaccg gctgggcggc
     2101 aagacccagc gcggccgcca gggccacggc gcgggtcagc aagcgggaaa tggtgcgcat
     2161 cgcccgcctt ttaccgccgc gcagcctgaa ccggcgctga agcgccgccg tgccgcgcga
     2221 ggaagtccag caccggatcg agcacccgcc gatcccggtt gaagggcgcg gcaagtttca
     2281 ggatcggtcc ggcatgatcg accccggcca gcaccacctg ctcgaccggc cggcccactg
     2341 catcgagcgc cctggccagg gcgcggctgt tgcggggctt gaccgtttcg tcggtatcgc
     2401 cggtcaagag cagcatcggc ggcgcgtcgg cgcggacgaa atggatcggc tgggtga
//
LOCUS       DASUDV010000109         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2013582,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000109 DASUDV010000000
VERSION     DASUDV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013582"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFV30_02390"
     CDS             <1..619
                     /locus_tag="VFV30_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HEX4846967.1"
                     /translation="ARLAAGHAAHDMLLWGARGMGKSALLRAAVLAAQAEGGRIALVQ
                     VAPEALGSLAALFAELGRQDRRFLVFLDDLGFEDHDSAGPRALRSWLEGGIEARPANV
                     RIAVTSNRRAIVPRHLSEQDDPINPRDAVDDRLALADRFGLSIGFHNCSQDDYLAIIA
                     GYCAALDLAWTEGEALEWSKRRGARSGRVAWQFVTELAGRAGKSL"
     gene            complement(621..2087)
                     /locus_tag="VFV30_02395"
     CDS             complement(621..2087)
                     /locus_tag="VFV30_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX4846968.1"
                     /translation="MSTKRKLLVALAVFLLVLGGFIFWIWRGTPAEHTTEQLSGRDPV
                     LAEPRPEQIPSIRIAKPVGWEAGEAPVAAEGLEVKRFAEGLDHPRVLYTLPNGDVVVA
                     EADSPTRNMGGGIQGWIAQKLMERAGSHGESPDRLVLLRDADGDGAAEARFTLLEGKH
                     LASPSGLAWADDRLYVANHDAVIVFDYKLGETALAGDPRKVMDLPAGGNHWMRNLVLS
                     ADTNRLYVAVGSASNIGERGMEEEKGRAAIWEKDLTKDGYPRRFAEGLRNPNGLAWNP
                     ASGELWTTVNERDMLGPDLVPDYLTNVPVGAHYGWPWIYWKKYDDDRVTSAPPEFIDD
                     FVRKPEYALGAHTAPLGLVFASGGERLGPAFANGAFIARHGSWNRKPAAGYDVVFVKF
                     DANGNPLGLPVPVLTGFLKDNGDTRGRPTWVTFAKDGALLVTDDTAGIVWRVIAPGAA
                     PSAAIKPVNVGRMPARRQLDGDPSQYRGAFKPDAQVKQ"
     gene            2148..2630
                     /locus_tag="VFV30_02400"
     CDS             2148..2630
                     /locus_tag="VFV30_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4846969.1"
                     /translation="MFDFQSDPDRPKAELYHDLDEAARAITAGEPDPVANMANVAALI
                     WQFVPQLNWAGFYRVSGDELVLGPFQGKPACIRIPFGKGVCGTAAQSGETQLVADVHA
                     FPGHIACDAASASELVVPVWRQDRVIAVIDLDSPAPGRFDAADAAGIEALAAAIAPAI
                     "
     gene            2758..3492
                     /locus_tag="VFV30_02405"
     CDS             2758..3492
                     /locus_tag="VFV30_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX4846970.1"
                     /translation="MLLRSALAGAAVLALAAPAFAQPAPPPPPPGVVDAAGNVYDGPM
                     PPHGARHPGPPPMARPMGHPGMPAQPGYDVAAWEQAKANWLAECRSRHGSDRKVGGAV
                     IGGIVGGVVGNRVAGRGNRTVGTVTGAAVGAVTGGAIGDAADKRAARDFCEAYLERNT
                     TWGPGYAQGQGVVGYGYAPMTVMVPVAYVATAAPAQARECTETVVTEEWVEVSRPQRV
                     RIIPPRPRPAPDKRVKIVPDKRVRTN"
     gene            complement(3560..>4106)
                     /locus_tag="VFV30_02410"
     CDS             complement(3560..>4106)
                     /locus_tag="VFV30_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4846971.1"
                     /translation="RNLDKIGQDEADTSELFFEDVRVPITNCLGQEGMGFIYLMSELP
                     QERLSIAVSAMASSQRAFDMTVEYTRERKAFGKPILDFQNSRFVLADLKAKLQVGWAH
                     LDWALARHLKKELTPEEGAAAKLWHTDLQWEVMDKCLQLHGGAGYMNEYPIARMWRGA
                     RVTRIFGGTNEIMKELIGRKL"
BASE COUNT          606 a         1426 c         1426 g          648 t
ORIGIN      
        1 cgcccggctg gcggcgggcc atgccgcgca cgacatgctg ctatggggcg cgcgcgggat
       61 gggcaagtcc gccctgctgc gcgccgcggt gctggccgcc caggccgaag gcggccggat
      121 cgcgctggtc caggtcgccc ccgaagcgct cggctcgctg gccgcgctgt ttgccgagct
      181 ggggcggcag gaccgccgct tcctggtctt cctcgacgat ctggggtttg aggatcacga
      241 cagcgccggg ccgcgcgcgc tgcgctcgtg gctggaaggc gggatcgagg cgcgcccggc
      301 caacgtccgc atcgcggtga ccagcaaccg ccgcgcgatc gtgccgcggc acctgtccga
      361 acaggacgat cctatcaacc cgcgtgacgc ggtggacgac cggctggccc tggccgaccg
      421 gttcggcctg tcgatcgggt tccacaactg cagccaggac gattacctgg cgatcatcgc
      481 cggctattgc gccgcgctgg acctcgcctg gaccgagggc gaggcgctgg aatggtccaa
      541 gcgccgcggc gcccggtccg gccgggtggc ctggcagttc gtgaccgagc tggccggacg
      601 ggccggtaag tccctctagc ttactgcttc acctgcgcgt cgggcttgaa cgcgccccgg
      661 tactgcgaag gatcgccatc gagctgccgc cgcgcgggca tccgcccgac attgaccggc
      721 ttgatcgccg ccgagggagc cgccccgggg gcgatgaccc gccagacgat cccggcggtg
      781 tcgtcggtca ccagcagcgc gccgtccttc gcaaaggtca cccaggtcgg gcggccgcgc
      841 gtgtcgccgt tgtccttgag gaacccggtc agcaccggaa ccggcaggcc cagcggattg
      901 ccgttggcgt cgaacttcac gaagaccacg tcatagccgg ccgccggctt gcggttccac
      961 gaaccgtggc gggcaatgaa ggcgccgttg gcaaaggctg gccccagccg ctccccgccg
     1021 ctggcgaaga ccaggccgag cggcgcggtg tgggcgccaa gggcgtattc gggcttgcgc
     1081 acgaaatcgt cgatgaactc cggcggggcc gaggtgaccc ggtcgtcgtc gtacttcttc
     1141 cagtagatcc acggccagcc gtaatgcgcg ccgaccggca cgttggtaag atagtcgggc
     1201 accagatcgg ggccgagcat gtcgcgctcg ttgaccgtgg tccacagctc gccgctcgcc
     1261 gggttccagg ccagcccgtt ggggttgcgc agcccttcgg caaagcggcg cggatagccg
     1321 tccttggtca ggtccttttc ccagatggcg gcgcggccct tctcttcctc catcccgcgt
     1381 tcgccgatgt tggaggccga gccgaccgcg acgtaaagcc ggttggtatc ggcgctgagc
     1441 accaggttgc gcatccagtg gttgccgccc gccggcaggt ccatcacctt gcgcggatcg
     1501 ccggccagtg cggtttcgcc cagcttgtag tcgaacacga tcaccgcatc gtggttggcg
     1561 acatagagcc ggtcgtccgc ccaggcgagg cccgaaggcg aggccaggtg cttgccttcc
     1621 agcagggtaa agcgcgcttc ggccgcgcca tcgccgtccg cgtcgcgcag caggaccagc
     1681 cggtcgggcg attcgccgtg cgaaccggcg cgttccatca gcttctgcgc gatccagccc
     1741 tggatcccgc cgcccatgtt gcgggtcggc gaatcggctt cggccaccac cacgtcgccg
     1801 ttgggcaggg tgtagagcac gcgcgggtga tcgagccctt cggcaaagcg ctttacctca
     1861 agaccttcgg ccgcgaccgg ggcttcgccg gcttcccagc cgaccggctt ggcgatccgg
     1921 atcgagggga tctgctcggg ccggggctcg gccagcaccg gatcgcggcc cgaaagctgc
     1981 tcggtggtgt gttcggccgg ggtgccccgc cagatccaga agatgaaacc gccgagcacc
     2041 agcaggaaca cggcgagggc gacaagcagc ttgcgtttgg tcgacatggc gccgtgatac
     2101 gatgtcaccg gctaggcggc aaccggactc tgggctaagg ccgcgcgatg ttcgatttcc
     2161 aatccgatcc cgaccgcccc aaggccgaac tctatcacga tctggacgaa gcggcccgcg
     2221 cgatcaccgc gggcgagccc gatccggttg ccaacatggc caatgtcgcg gcgcttatct
     2281 ggcaattcgt gccgcagctg aactgggccg ggttctaccg ggtcagcggc gatgaactgg
     2341 tcctgggccc gttccagggc aagccggcct gcatccgcat tccctttggc aagggcgtct
     2401 gcggcaccgc ggcgcagagc ggcgagaccc agctggtggc cgatgtccac gccttccccg
     2461 ggcacatcgc ctgcgatgcg gccagcgctt cggaactggt cgtgccggtc tggcgtcagg
     2521 accgggtgat cgcggtgatc gatctcgaca gcccggcccc gggccggttc gacgccgccg
     2581 acgccgccgg gatcgaggcg ctggcggcgg ccatcgcccc ggcaatctaa gcccccggcg
     2641 ctgctccgaa acgggacagc ggcaagagag ccgcggagcg agccttggtc gcgggccggt
     2701 tgtgatgcta aattccgggt taacaccgga atcatccggt cgatccaggg gattgccatg
     2761 cttcttcgtt ctgcccttgc cggtgccgcc gtgctggcgc ttgccgcgcc ggcctttgcc
     2821 cagcccgccc cgccgcctcc gccgccgggc gtggttgacg ccgccggcaa cgtctatgac
     2881 ggcccgatgc cgccgcatgg cgcccgccat cccggcccgc cgccgatggc ccggccgatg
     2941 ggtcaccccg gaatgccggc ccagccgggc tatgacgtgg cggcctggga acaggccaag
     3001 gccaactggc tggccgagtg ccgcagccgt cacggcagcg accgcaaggt tggcggcgcg
     3061 gtgatcggcg ggatcgttgg cggcgtggtg ggcaaccggg tggccgggcg cggcaaccgg
     3121 acggtcggca ctgtcaccgg cgcggcggtt ggcgcggtca ccggcggtgc gatcggcgat
     3181 gccgccgaca agcgcgccgc gcgcgatttc tgcgaagcct atctggaacg caacaccacc
     3241 tggggtccgg gctatgccca gggccagggc gtggtcggct atggctatgc gccgatgacc
     3301 gtgatggttc cggtcgccta tgtcgcgacg gccgccccgg cccaggcgcg cgagtgcacc
     3361 gaaaccgtgg tgaccgagga atgggtcgaa gtgtcgcgcc cgcagcgcgt ccgcatcatc
     3421 cccccgcgtc cgcgccccgc gcccgacaag cgggtgaaga tcgtgccgga caagcgagtc
     3481 cgcaccaact gattgaaaag acgaaaaaag aagggggcca accggccccc ttcgcttatc
     3541 cggcagagcg gccgcagggt cagagcttgc ggccgatcag ttccttcatg atttcgttgg
     3601 tcccgccgaa gatccgggtc acgcgggccc cgcgccacat ccgggcgatc gggtactcgt
     3661 tcatgtagcc ggccccgccg tggagctgga ggcacttgtc catcacttcc cactgcaggt
     3721 cggtgtgcca cagcttggcc gcggcgcctt cctccggcgt cagctccttc ttcaggtggc
     3781 gcgccagggc ccagtcgagg tgggcccagc caacctgcag cttggccttg agatcggcca
     3841 ggacaaaccg gctgttctgg aaatcgagga tcggcttgcc gaaagccttg cgctcacggg
     3901 tgtactccac cgtcatgtcg aaggcgcgct gcgaactggc catggccgag accgcgatcg
     3961 agagccgctc ctgcggcagc tcgctcatca gatagatgaa gcccatgcct tcctggccca
     4021 ggcagttggt gatcggcacc cgcacgtcct cgaagaacag ctccgaggta tcggcctcgt
     4081 cctgcccgat cttgtcgagg ttgcgg
//
LOCUS       DASUDV010000110         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1726449,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000110 DASUDV010000000
VERSION     DASUDV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726449"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VFV30_02415"
     CDS             complement(<1..1087)
                     /locus_tag="VFV30_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4846972.1"
                     /translation="MTGRLSTLAAAFVIARRDFTAILFSRSFFFFLLGPLFPILVGGL
                     AGGIGREVQSNAARPELGVAMSAADAKALLAARERLEPQLGSAVPRLVVVKEIRPGED
                     FDPAAAMQDKRVNLAAVLTGTLERPMLSGPEERIAGWRGPVAVLAGEAQRASGAAYPT
                     VDLTPLKLATTADETRSRTVTAQLGQTVLFLLTMLLAGMVLSNLVEEKGNKIIEVLAA
                     AIPMDAVFLGKLFAMLAVSFVGIAVWATAGSAVWLGSGGNLSQYPAPAVGWPMFFLLG
                     TAYFAMGYLLLGSLFLAIGSMAATVREVQTLSMPVTMLQLLNFFFASWAMAKPGTLIE
                     FAAMAVPFSSPFAMLARAAQSEALLPHA"
     gene            complement(1089..2018)
                     /locus_tag="VFV30_02420"
     CDS             complement(1089..2018)
                     /locus_tag="VFV30_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4846973.1"
                     /translation="MAIEAKGLVKRFDGFTAVDGVDISVPQGAIYGILGPNGAGKTTT
                     LRMLLGIIDPDEGLRRVLGHDRPHEISHAIGYLPEERGLYPAMKAYEAIAFLGALRGL
                     PLAEGRQRGKELLEEHGLGYAIDRQIRQLSKGMAQQVQLLGTLVHEPRLVVFDEPFSG
                     LDALNQGKLERMIRGLAAKGTTVIFSTHVIAHAERLCDEVAIIAGGKVPFAGPVDVAR
                     DRIRPQVRLETRARDGAWRKALPEDARHEGNYWFFSLPESGIEPLLRALIEGEAGILS
                     LSIERAGLHDAFVAIAGEAAARALEEGNGEEAR"
BASE COUNT          361 a          707 c          653 g          325 t
ORIGIN      
        1 gcgcgtgcgg cagcagcgcc tcgctctgcg cggcgcgggc cagcattgcg aagggcgagc
       61 tgaacggcac cgccatcgcc gcgaattcga tcagcgtgcc cggcttggcc atcgcccacg
      121 atgcgaagaa gaagttcagc agctggagca tggtgaccgg catcgacagc gtctgcacct
      181 cacgcacggt cgccgccatc gaaccgatcg ccaagaatag cgagccgagc agcaggtaac
      241 ccatcgcgaa ataggccgtg ccgagcagga agaacatcgg ccagccgact gccggggccg
      301 gatactggct gaggttgcct cccgagccaa gccagaccgc agagcccgcc gtcgcccaga
      361 ctgcaatccc gacgaaggac accgccagca tcgcgaacag cttgcccagg aacaccgcat
      421 ccatcggaat cgccgcagcc aggacttcga tgatcttgtt gcccttttcc tcgaccaggt
      481 tggagaggac catgcccgcc aacagcattg tcagcaggaa cagcacggtc tggccaagtt
      541 gcgcggttac cgtgcggctg cgcgtttcgt cagccgtggt agcaagtttc aatggggtca
      601 gatctaccgt tggataggct gcgccgctgg cgcgttgcgc ctcgcccgcc agcaccgcga
      661 ccgggccgcg ccagccggcg atccgctctt cggggcccga cagcatcggc cgctcgagcg
      721 tgccggtcag cacggcggcg aggttgaccc gcttgtcctg catcgccgca gcaggatcga
      781 agtcttcgcc cgggcggatt tccttgacca cgaccaggcg cggcacggcg ctgcccagtt
      841 gcggttcgag ccgttcgcgc gctgctagca atgccttggc atcggcggcc gacatcgcca
      901 cgcccaattc gggccgggcg gcattgctct gcacttcgcg gccgatcccg ccggccagtc
      961 ctccgacaag gatcgggaac agcggcccga gcaggaagaa gaagaacgag cgcgaaaaca
     1021 ggatcgcggt gaaatcgcgc cgggcgatga cgaaggcagc ggccagggtg gaaagtcgtc
     1081 cggtcatctc agcgggcctc ctcgccattg ccttcttcga gcgcgcgcgc cgccgcctcg
     1141 cccgcgatcg cgacgaaggc atcgtgcagc ccggcgcgtt cgatcgagag cgagaggatc
     1201 cctgcctcgc cttcgatcag cgcgcgcaac agcggctcta tcccgctttc ggggagggag
     1261 aagaaccagt aattgccttc gtgtcgtgca tcttccggaa gcgccttgcg ccacgccccg
     1321 tcgcgggccc gcgtctcgag ccggacctgc ggccggatcc ggtcacgcgc cacgtcgacc
     1381 ggcccggcga aaggcacctt gcccccggcg attatcgcca cttcgtcgca cagccgttcg
     1441 gcatgagcga tcacgtgggt cgagaaaatc acggtcgtgc ccttggcggc aagcccgcgg
     1501 atcatccgct ccagcttgcc ctggttgagc gcgtcgaggc cggagaacgg ctcgtcaaac
     1561 accaccagtc gcggttcgtg gaccagggtg ccgagcaatt gcacctgctg ggccatcccc
     1621 ttcgaaagct ggcggatctg gcggtcgatc gcatagccca ggccatgttc ctcgagcagt
     1681 tccttgccgc gctggcgccc ttcggccagc ggcagcccgc gcagcgcgcc caggaaggca
     1741 atcgcttcat aggccttcat cgccggataa agcccgcgct cttcgggcag gtagccgatc
     1801 gcgtggctga tctcgtgtgg gcggtcatgc cccaggacgc ggcgcaggcc ttcatcggga
     1861 tcgataatgc caagcagcat tctgagcgtg gtggttttcc ccgcaccatt ggggccgaga
     1921 atgccgtaga tcgccccttg agggaccgaa atgtccaccc catcgacggc cgtgaacccg
     1981 tcaaagcgct tgaccaggcc cttggcctcg attgccagcg gtcgtcccgc gccttcaact
     2041 gatccg
//
LOCUS       DASUDV010000111         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_864944,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000111 DASUDV010000000
VERSION     DASUDV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864944"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFV30_02425"
     CDS             complement(<1..697)
                     /locus_tag="VFV30_02425"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX4846974.1"
                     /translation="MSDIKRVVLAYSGGLDTSVILKWLQVTYNCEVVTFTADLGQGEE
                     LEPARAKAKLMGVPDHHIYIDDLREEFVRDFVFPMMRANARYEGDYLLGTSIARPLIS
                     KRLVEIAHETGADAVAHGATGKGNDQVRFELSAYALDPSIKVIAPWREWDLTSRTALI
                     AWAEAHQIPVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTDNPENAP
                     DTPEYITIDFERGD"
     gene            802..1593
                     /locus_tag="VFV30_02430"
     CDS             802..1593
                     /locus_tag="VFV30_02430"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846975.1"
                     /translation="MTDWIEIAEPHDLTPWQRPLRDPPAGLAAAEGRGVQAAPGPFTP
                     FARMTDPGGDGVGDGVWRHPDGTLTVACTTPMAGVTAAMWDWWFGWHSLSSPRYRLWH
                     PQAHRKSQLAEDRRHLPPGRARYIGNVSAVDEFIGDQMTRLAIGFVPPAAFGFDPDRV
                     DALGTAVCATVALRAERIEHGQLVHLLENTAEGCVMHSRFHLGRFRSRVPLAGPLLTR
                     LIDRPAVRRRLVSDAIGLALLRHCFEEMHHLAGILADLHRHFKED"
     gene            1598..2035
                     /locus_tag="VFV30_02435"
     CDS             1598..2035
                     /locus_tag="VFV30_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX4846976.1"
                     /translation="MAKADLKTKPTALSVADFIAAVPDERRRAEAAEIDALHRRVTGC
                     EPQLWGPSIIGYGSYNYKYDSGREGTMCRGGFSPRKAAMTVYLMGNYVDRQAEADDLF
                     ARLGKHTTGKSCLYIKKLADVDRTVLERLVALSWEIMNAKYPD"
     gene            2085..3383
                     /locus_tag="VFV30_02440"
     CDS             2085..3383
                     /locus_tag="VFV30_02440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4846977.1"
                     /translation="MAGKAPPERNRYVDFLRALSILAVVIGHWLVAAPYMKDGAVVGG
                     HLLGILPWTQWLTWSFQVMPLFFCVGGFSNGVSWAATQAKGGTFSDWFGGRVQRLINP
                     VLPLFLVWSLFALAGTALGVDRAVVRMAAELALIPVWFLAVYLIVSALVPITHAAWRR
                     FGMGSFWALVAKAVAVDLLSVGMGVPVVNWLNFLFVWLAIHQLGYAWQEGRFRQPLLW
                     GLTALAVLGALVWFGPYPVAMIGVPGSDLSNSMPPTLALLALGNMQIGFALAAEQPMR
                     RLLTRAWLWTATVLVNGMIMTVYLWHLTAFVLVMVAAWLLGGVGLSAVPGSGEWWLGR
                     PVWFALYVAVLFPLIGLFSPFERMGSQAGKDGVKHVPRWRLVLGLLLICLGLALTAAI
                     SIASPEGVTGLRVWVVALPFVGAGLVGFGPAYTLAKRFDR"
     gene            complement(3380..>4677)
                     /locus_tag="VFV30_02445"
     CDS             complement(3380..>4677)
                     /locus_tag="VFV30_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846978.1"
                     /translation="AIDLRDDDRFPVSMMGEKWANLVCGDADDSPIPITEKTRQGCAR
                     RKVERDRMEEEIELSFKLEGDPALRDRLCRRTPAGQRTEVQNAACQSAESQLAWRKER
                     ADRDREAARLLANPTALDKQCAGVKIGRNDIAATALGEACQKRAEVLRDRETERLVKV
                     MERDPGEYTRLCHWDYPQITSEDPVEVACERIQSDKEDAARQRDYAELDKLSPAQLEA
                     QCQALAAEEDRKRAAAKPGEPIESNYTAFSAACDSLASTRREARWKALQADPAKLAEA
                     CAKATEETDHDLADRCADQKAWEEEERLDRQAWALAKDHAALVSACQSTPHDARGPVL
                     AMACERYRKCLILPVGTRIPEKNMIRADWVRMGSEEESARLFYEGRFGDGHGAFCYET
                     VEEANTAWELVKDPAKRVIDQTATYNGYPVPRPKPAKRN"
BASE COUNT          640 a         1512 c         1647 g          878 t
ORIGIN      
        1 catcgccgcg ttcaaaatcg atcgtgatgt attccggggt gtccggggcg ttctccggat
       61 tgtcggtgcg cgaatataca tagtccgggg tctcttccca cggatcttcc agcaccttgc
      121 cctcgctgga ggtgtgcagc aggttggcgt cggtcgagaa cgggctttcg ccgcgcttgt
      181 ccttgggcac cgggatctgg tgcgcttcgg cccaggcgat cagcgcggtg cggctcgtca
      241 ggtcccattc gcgccaggga gcaatgacct tgatgctggg atcgagcgca taggccgaca
      301 gttcgaaccg cacttggtcg ttgcccttgc cggtggcgcc gtgggccacg gcatcggcgc
      361 cggtttcgtg cgcgatctcc accagccgct tggaaatcag cgggcgggcg atcgaggtgc
      421 cgagcaggta atcgccttcg taacgggcgt tggcgcgcat catcgggaag acgaaatcgc
      481 gcacgaactc ctcgcgcagg tcgtcgatgt agatgtggtg atcgggcacg cccatcagct
      541 tggccttggc gcgggccggt tccagctcct cgccctggcc caggtcggcg gtgaaggtca
      601 ccacttcgca gttataggtc acctgcagcc acttcaggat caccgaggtg tcgaggccgc
      661 ccgaataggc aagaacgacg cgcttgatat cggacatgat cgacgactcc cgctggaaac
      721 tgcggggcgc ggctagcagg cggggggccg gggtgcaacg attgcgttgg ccaaagccgg
      781 gccttgggtt agcctttgcc aatgaccgac tggatcgaga tcgccgagcc gcacgatctg
      841 acgccctggc agcgccccct gcgcgatccg ccggcgggcc tggccgcggc cgaaggcagg
      901 ggcgtgcagg ccgcgccggg cccgttcacg ccctttgccc ggatgaccga tcccggcggc
      961 gacggggtgg gcgatggggt ctggcggcat cccgacggaa ccctgaccgt ggcctgcacc
     1021 acgccgatgg cgggggtgac ggcggcgatg tgggactggt ggtttggctg gcacagcctg
     1081 tcgagcccgc gctaccgcct gtggcatccg caggcgcacc ggaaatcgca gctggccgag
     1141 gaccgccgcc acctgccgcc gggccgcgcg cgctacatcg gcaatgtcag cgcggtggac
     1201 gagttcatcg gtgaccagat gacccgcctg gccatcggct tcgtgccgcc cgccgcattc
     1261 gggttcgatc cggaccgggt cgacgcgctg ggcaccgcgg tttgcgccac ggtggcgctg
     1321 cgggccgaac ggatcgagca cggccaactc gtccacctgc tggagaatac ggccgaaggc
     1381 tgcgtcatgc acagccgctt tcatctgggg cggttccgca gccgcgtgcc gctggccggg
     1441 ccgctgctga cccggctgat cgatcgcccg gcggtgcggc gcaggctggt cagcgatgcg
     1501 atcgggctgg cactgctgcg ccactgcttc gaggaaatgc accacctggc cgggatcctg
     1561 gccgatcttc accggcactt caaagaggat tgagccgatg gccaaagcag acctgaagac
     1621 caagccgacc gcgctcagcg tcgcggactt tatcgccgcc gtgccggacg aacgccgccg
     1681 cgccgaagcg gccgagatcg acgcgctgca tcgcagggtg acgggctgcg agccgcagct
     1741 gtggggcccg tcgatcatcg gctatggcag ctacaactac aagtacgaca gcggccgtga
     1801 ggggacgatg tgccgcggcg ggttcagccc gcgcaaggcg gcgatgacgg tctatctgat
     1861 gggcaactat gtcgaccgac aggccgaggc cgacgacctg ttcgcccggc ttggcaagca
     1921 caccaccggc aagagctgtc tctacatcaa gaagctggcc gatgtggata ggacggtgct
     1981 ggaacggctc gtggccttga gctgggagat catgaacgcc aagtatccgg actgacgcgg
     2041 ttttcggggg gacggccatg gggatgtgga gcaaggcggc agagatggcc ggaaaggccc
     2101 cgcccgagcg gaaccgctat gtcgatttcc tgcgggcgct gtcgatcctg gccgtggtta
     2161 tcggccactg gctggtcgcc gcgccctata tgaaggacgg ggcggtggtc ggcgggcacc
     2221 tgctgggtat cctgccgtgg acccaatggc tgacctggag ctttcaggtg atgccgctgt
     2281 tcttctgcgt cggcggcttt tccaacggcg tctcctgggc cgcgacgcag gccaagggcg
     2341 ggaccttttc cgactggttc ggcggacggg tgcagcgcct gatcaacccg gtgctgccgc
     2401 tgttcctggt ctggagcctg tttgccctgg ccggaaccgc gctgggcgtg gaccgcgcgg
     2461 tggtgcggat ggcggccgaa ctggcgctga tcccggtctg gttcctggcg gtctacctga
     2521 tcgtctcggc gctggtgccg atcacccatg ccgcctggcg gcgcttcggc atgggcagtt
     2581 tctgggcgct ggtcgccaag gccgtggcgg tcgacctcct gtcggtcggg atgggcgtgc
     2641 cggtggtcaa ctggctcaac ttcctgttcg tctggctggc gatccaccag ctcggctatg
     2701 cctggcagga agggcgcttt cgccagccgc tgctgtgggg cctgactgca ctggcggtgc
     2761 tgggcgctct ggtctggttc gggccctatc cggtggcgat gatcggcgtg ccgggcagcg
     2821 acctgtccaa ctccatgccg ccgaccctgg cgctgctggc gctgggcaac atgcagatcg
     2881 gctttgccct ggcggccgag cagccgatgc ggcggctgct cacccgggcc tggctgtgga
     2941 ccgccaccgt gctggtcaac ggcatgatca tgaccgtcta cctctggcat ctgaccgcct
     3001 tcgtgctggt gatggtggcc gcctggctgc tgggcggagt ggggctgagc gccgtgccgg
     3061 gcagcggcga gtggtggctg ggccgcccgg tctggttcgc gctctacgtg gccgtgctgt
     3121 tcccgctgat cggcctgttc agcccgttcg agcggatggg cagtcaggcc ggcaaggacg
     3181 gggtgaagca cgtgccgcgc tggcgtctgg tcctgggcct gctgctgatc tgcctgggcc
     3241 ttgccctgac cgccgcgatc agcatcgcca gccccgaggg tgtcacgggc ctcagggtct
     3301 gggttgtggc gctgccgttc gtgggcgccg ggctggtcgg gtttggcccg gcctacaccc
     3361 tcgccaaacg gtttgaccgt tagttgcgct tggccggctt ggggcggggg acggggtagc
     3421 cgttgtaggt cgccgtctgg tcgatcaccc gtttggcggg gtccttgacc agttcccagg
     3481 cggtattggc ctcttccacc gtctcgtaac agaacgcgcc gtgtccgtcg ccgaagcggc
     3541 cttcatagaa caagcgggcg gattcctctt ccgatcccat ccgcacccaa tccgcgcgga
     3601 tcatgttctt ttccgggatg cgggtgccga ccggcaggat cagacacttg cgataccgtt
     3661 cgcaggccat ggccagcacc ggaccacgcg cgtcgtgcgg ggtggactgg caggctgata
     3721 cgagcgcggc gtggtccttg gccaacgccc aggcctgccg gtcgagccgc tcctcctcct
     3781 cccaggcctt ctggtcggcg cagcgatcgg caagatcgtg atcggtttcc tcggtcgcct
     3841 tggcgcaggc ttcggccagc ttggccggat cggcctgcaa ggccttccac cgcgcttcgc
     3901 ggcgcgttga ggccaggctg tcacaggcgg cggagaacgc cgtgtaattg ctctcgatcg
     3961 gttcaccggg cttggcggcg gcgcgcttgc ggtcttcctc ggcggcgagg gcctggcact
     4021 gggcttcaag ctgcgccggt gagagcttat ccagctccgc atagtcgcgt tggcgtgcgg
     4081 catcttcctt gtctgactgg atccgttcgc aggcaacctc gaccgggtct tcgctggtga
     4141 tctggggata gtcccagtgg cacagccggg tgtactcgcc cggatcgcgc tccatgacct
     4201 tgaccagccg ttcagtctcg cgatcgcgca gcacttcggc gcgcttctgg caggcctcgc
     4261 ccagggccgt tgcggcaatg tcgttgcggc cgatcttcac cccggcgcat tgcttgtcga
     4321 gcgcggtggg attggcgagc agccgggccg cttccctgtc gcggtctgcc cgctccttgc
     4381 gccaggcaag ctgactttcg gcgctttggc aggccgcatt ctgcacttcc gtgcgctggc
     4441 cggccggagt gcggcggcag agcctgtcgc gcagcgccgg atcgccttcc agcttgaacg
     4501 acagttcgat ctcttcctcc atccggtcgc gctcgacctt gcgcctggcg cagccctgac
     4561 gggtcttctc ggtgatcggg atcggcgaat cgtcggcatc cccgcagacc agattggccc
     4621 acttctcacc catcatcgaa accggaaagc ggtcatcatc gcgcaggtcg atggcct
//
LOCUS       DASUDV010000112         7332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_842856,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000112 DASUDV010000000
VERSION     DASUDV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842856"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1597
                     /locus_tag="VFV30_02450"
     CDS             23..1597
                     /locus_tag="VFV30_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX4846979.1"
                     /translation="MEDIFIGLGANGERQTLRLGRANRHGLIAGATGTGKTVTLQTLA
                     EQFSAQGVPVFMADVKGDLAGISMPGSSTFKHADKIEGRAKELGIEPFEYRDNPAVFW
                     DLYGEQGHPIRTTISEMGPLLLSRLMGLNETQEGVLTIAFKWADDNGLLLLDMDDLQQ
                     TLIACAEHASELATTYGNISKASVGTIQRQLLAFESQGADRFFGEPAFEINDFIRCDE
                     TGRGVINVLAADKLMQSPKLYATFLLWLLAELFEALPEVGDPDKPKLVFFFDEAHLLF
                     EDAPEALFDKVEQVVRLIRSKGVGVYFISQNPIDIPEKIAGQLGNRVQHALRAFTPRD
                     QKAIKAAAETFRINPDLDVETAITELRTGEALVSTLDEEGAPTIVQRTLVAPPRSRLG
                     PITPKERAIIQSISPFDGKYDSAVNRESAAEVLAKKAEDAAAAAQVVEEQGEEAQRAQ
                     PRATPSMWEKAGKAAFGAAAASAGTMVARKITGRKSSGSPMASAASAAAGAIGTALGG
                     ATLGRFARGLLGSLLR"
     gene            1697..4369
                     /locus_tag="VFV30_02455"
     CDS             1697..4369
                     /locus_tag="VFV30_02455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX4846980.1"
                     /translation="MRALLFAASAITLALTTAAPASAPAADAAAEAARALAPVPPGKL
                     TDAVRPLAYRIDLTVDPNQERFSGKVEIDTVLKRPSRFIDLHGRDLKMGSAVAVVNGQ
                     RIPARYFELDPTGVARLTFDQALPAGPVTLAFEYDAPFGSSPAGMFRVKVGEDWYSWT
                     QFQSIDARAAFPGFDEPGFKVPFTVTLRTRPGHMAVSNAPQISKTTENGMEVHRFAPT
                     LPLPTYLMAFMVGPFVAVDSTVAPTPQRNWPLPVRIITTKPNAAKMDFAMRGTKDIVR
                     HLESYFGQAFPYPKLDQITTPILPGAMENAGADLYNDSILILDDNATTRQKRTFGMVV
                     AHELAHQWFGDLVTPAWWDDIWLNESFANWMGFRIGHEWRPDLNIRAGALAEGFGAMN
                     TDALVAGRAIHQPIETNAQIDEAFDSITYGKGGHVVAMIAGYMGDTKFRDGVRAYMKA
                     HEHGSATSADFFGAMAKVSGDPRILPAMQSFTDQQGVPLVTFAADGKGGYTVTQSRYA
                     RLGATPPDTRWGIPLCVRRVTAKQQCQLLDQRQGTVRAVGRGPLIPNAGGTGYYRFEL
                     PARDWDALIAIAGKLPGGEALALDDSLFASFQAGRASPDQLIAGARQLATNPDSYASA
                     SGMDSLQWFYNAGMLDAAGEAGYRRLVGQVYGPRLAAMGFDPRAAAYAAEDPEKSQMR
                     AEAVGQLAGIARDPALRKQLGDAAKAYLAGDAKALDAAWFRTAFAILVEEGGLDMAKR
                     LADTAMGTQDALLRGAALGSVTTSGKEEIARWVLTDFNDPRLRRSEKLGLIRGIVGTR
                     GTRDFGFAWLKDNYDALSAGGGGIFFTSGLPNLVGGYCSVAKADEIAAFLRPKLAGKT
                     GALSLERTIERVRSCGVLKDARGAELSAAFGKVK"
     gene            complement(4379..5806)
                     /locus_tag="VFV30_02460"
     CDS             complement(4379..5806)
                     /locus_tag="VFV30_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4846981.1"
                     /translation="MMQRLRGLLWPRSLQGQLLLAVALALLLAQGISAVLLYRAQMDR
                     REAALIHTAALRLFGAAREEGLIGTPPRGNPDPRPPRPEGDRQLGVEAGSARPDLGTG
                     RRHSRAEEDLREIIEGQGLDLGEVVVVERPLSDDPRLLRRLERRARLLGRDPERPQRL
                     LAAAVELPAAKRWLTVRVPVPKGEPALIFTLLGQTLLLYALMVGAIALIVGRITRPLA
                     ALTTRLERFAAHRDTHGQLEPSGPDDMRRLILAHNAMEDRIAALLDEKDVMLGAIGHD
                     LKTPLAALRVRIESVEDPTERARMAATIEDITRSLDDILSLARVGRPSDPLEPTELNA
                     LVGQVAEEFEDLGEPVVFTPAARMVLPLRATWLRRAVRNLAANALRYGGAARIALTAE
                     HGAAVIRIEDDGPGIPEDRIEAMMQPFTRGEPSRNSGTGGAGLGLTLARAIAEQHGGT
                     LTLTNRPGADGSVAGLTATLVLPLS"
     gene            complement(5803..6537)
                     /locus_tag="VFV30_02465"
     CDS             complement(5803..6537)
                     /locus_tag="VFV30_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4846982.1"
                     /translation="MDQSDATRLLLVDDEAALREPLAEYLARQGFVVHQAASAAEART
                     RLRDDPPDLVLLDIMMPGEDGLSLCRHLAETQAVPTILLTARTEATDRIVGLEIGADD
                     YVTKPFEPRELVARIRSVLRRASKGAPAQTDEEHLLFDGWRLDPLRRKLTDPEGAVVA
                     ISSVEFRLLMAFLEHPRQVLDRDRLLDMVQGREAHLFDRAVDNQVSRLRRKIEVDSRN
                     PLLIQTVWGGGYMLAADVRREPAARE"
     gene            complement(6561..7172)
                     /locus_tag="VFV30_02470"
     CDS             complement(6561..7172)
                     /locus_tag="VFV30_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX4846983.1"
                     /translation="MKTSLIAFALLSTGLATAAVAQSGPADADPLGGKTVTRAEMMAQ
                     AGTQFDQMDTNKDGKLDRADRAAEHGKKFDAMDTNKDGSLSRAEFDAAHQAMGEKRGE
                     RGKRGEGRMGGKRGEGRMAGMMLRMADANKDGAVSREEFQAARGKMFDTADSNKDGKL
                     TPEERKAHHAQMRERMGKRGQRGPGGHGDHGAHGDMPPPPSAN"
BASE COUNT         1100 a         2550 c         2555 g         1127 t
ORIGIN      
        1 actcacgcgg agcttgcccc ccatggaaga catcttcatc ggccttggcg ccaacggcga
       61 acgccagacc ctgcgccttg gccgcgccaa ccgccacggc ctgatcgccg gggcgaccgg
      121 caccggcaag accgtgaccc tccagaccct ggccgagcag ttctctgccc agggcgtgcc
      181 ggtgttcatg gccgacgtga agggcgatct ggccggcatc tcgatgcccg gcagcagcac
      241 cttcaagcac gccgacaaga tcgagggccg ggccaaggag ctggggatcg aaccgttcga
      301 ataccgcgac aatccggcgg tgttctggga tctctatggc gagcagggcc acccgatccg
      361 caccaccatc tcggaaatgg ggccgctgct gctcagccgg ctgatgggcc tcaatgaaac
      421 ccaggaaggc gtgctgacga tcgccttcaa gtgggccgac gacaacggcc tgctgctgct
      481 cgacatggac gacctgcagc agaccctgat cgcctgcgcc gaacacgcca gcgaacttgc
      541 caccacctat ggcaacattt ccaaggcctc ggtcggcacg atccagcgcc agctgctggc
      601 cttcgaaagc cagggcgcgg accggttctt tggggagccg gccttcgaga tcaacgattt
      661 catccgctgc gacgagaccg ggcgcggcgt gatcaacgtg ctcgccgccg acaaactgat
      721 gcagagcccc aagctctacg ccaccttcct gctgtggctg ctggccgaac tgttcgaggc
      781 gctgcccgaa gtgggcgatc cggacaagcc gaagctggtg ttcttcttcg acgaggccca
      841 cctgctgttc gaggacgcgc ccgaggccct gttcgacaag gtggagcagg tcgtccgcct
      901 gatccggtcc aagggcgtcg gcgtctattt catcagccag aacccgatcg acattcccga
      961 aaagatcgcc gggcagctcg gcaaccgggt ccagcacgcc ctgcgcgcct tcaccccgcg
     1021 cgaccagaag gcgatcaagg ccgcggccga gacgttccgg atcaacccgg acctcgatgt
     1081 cgaaaccgcg atcaccgagc tcaggaccgg ggaagcgctg gtctccaccc tcgacgagga
     1141 aggcgcgccg accatcgtgc agcgcaccct ggtggcgccg ccgcgctcgc gcctggggcc
     1201 gatcacgccc aaggagcgcg cgatcatcca gtcgatcagc ccgtttgacg gcaagtacga
     1261 cagcgcggtg aaccgcgaaa gcgccgccga agtcctggcg aagaaggccg aggatgccgc
     1321 tgccgccgcg caggtggtgg aagagcaggg cgaagaggcc cagcgcgccc agccgcgcgc
     1381 cacccccagc atgtgggaaa aggccggcaa ggccgccttc ggtgccgccg cagcttcggc
     1441 cggaaccatg gtggcgcgca agatcaccgg ccgcaaatcg agcggatcgc cgatggcctc
     1501 tgccgccagc gccgcggccg gggcgatcgg caccgcgctg ggcggggcaa ccctgggccg
     1561 ctttgcccga gggctgctgg gcagcttgct gcgttgacgc cggtcggcct tgctcggcgg
     1621 ttggtcgagc agggcgcttg caaatcgcgc gcgcgtgggg tctgaggcgg tcataaggtc
     1681 gccttccgga gaacccatgc gcgcactgtt gtttgcggct tcggccatta cgcttgccct
     1741 gaccaccgcg gcaccggcca gtgcccccgc cgccgatgct gccgccgagg cggcgcgcgc
     1801 ccttgcccct gttccgccgg gcaagctgac cgacgcggtc cggccgctgg cctaccggat
     1861 cgacctcact gtcgatccga accaggagcg cttctccggg aaggtggaga tcgacaccgt
     1921 gctgaagcgc cccagccgct tcatcgacct gcacgggcgc gacctgaaga tgggcagcgc
     1981 ggttgccgtg gtgaacggcc agcgcattcc ggcgcgctat ttcgagctcg atcccaccgg
     2041 cgtggcccgg ctgaccttcg atcaggcgct gccggccggc ccggtcaccc tggcctttga
     2101 atacgacgcg ccgttcggct ccagcccggc cgggatgttc cgggtcaagg tgggcgagga
     2161 ctggtacagc tggacccagt tccagtcgat cgatgcccgc gccgccttcc cgggcttcga
     2221 cgagcccggc ttcaaggtgc ccttcaccgt gacgctgcgg acccgcccgg ggcacatggc
     2281 ggtaagcaac gcgccgcaga tttcgaagac gaccgagaac gggatggagg tgcaccgctt
     2341 tgcccccacc ctgccgctgc cgacctatct gatggccttc atggtcgggc ccttcgtggc
     2401 ggtggacagc acggtcgcgc ccaccccgca gcgcaactgg ccgctgccgg tgcggatcat
     2461 caccaccaag cccaacgccg cgaagatgga cttcgcgatg cgcgggacca aggacatcgt
     2521 ccgccacctg gaaagctatt tcgggcaggc cttcccctat cccaagctgg accagatcac
     2581 gacgccgatc ctgcctggcg cgatggagaa cgccggggcg gacctctaca acgattcgat
     2641 cctgatcctc gatgacaacg ccaccacccg ccagaagcgg accttcggca tggtcgtggc
     2701 gcacgagctg gcgcaccagt ggttcggcga tctcgtcacg ccggcctggt gggacgatat
     2761 ctggctgaac gaaagcttcg ccaactggat ggggttccgg atcggccacg aatggcggcc
     2821 cgatctcaac atccgggccg gggccctggc cgaagggttc ggggcgatga acaccgacgc
     2881 tctggtcgcc ggccgcgcga tccaccagcc gatcgaaacc aacgcccaga tcgacgaggc
     2941 gttcgattcg atcacctatg gcaagggcgg ccacgtggtg gcgatgatcg ccggctacat
     3001 gggcgatacc aagttccgcg acggggtgcg cgcctacatg aaggcgcacg agcacggcag
     3061 cgcgaccagt gccgacttct tcggagccat ggccaaggtt tcgggcgatc cgcggatcct
     3121 gcccgcgatg cagagcttta ccgatcagca gggcgtgccg ctggtgacct tcgcggccga
     3181 cggcaagggc ggctacaccg tcacccagag ccgctatgcc cgccttggcg ccacgccgcc
     3241 ggacacccgc tggggcattc cgctgtgcgt ccgccgggtc accgccaagc agcagtgcca
     3301 gctgctggac cagcgccagg gcacggtccg ggccgtgggg cgcgggccgc tgatccccaa
     3361 cgcgggcggc accgggtatt accgtttcga actgccggcg cgcgattggg acgcgctgat
     3421 cgccattgcc ggcaagctgc cgggcggcga ggcgctggcg cttgacgatt cgctgttcgc
     3481 cagcttccag gccggccggg ccagcccgga ccagctgatc gccggtgcgc gccagctggc
     3541 gaccaatccg gacagctatg ccagcgcttc gggcatggac agcctgcagt ggttctacaa
     3601 cgccgggatg ctcgatgccg cgggcgaggc cggctaccgc cgcctggtgg gccaggtcta
     3661 tggcccgcgc ctggccgcga tgggctttga tccgcgcgcc gcggcctatg ctgccgagga
     3721 tccggaaaag tcgcagatgc gggccgaagc cgtcggccag ctggccggga tcgcccgcga
     3781 tccggcgctg cgcaagcagc tgggcgatgc tgccaaggcc tatctggccg gcgatgccaa
     3841 ggcgctggat gcggcctggt tccgcaccgc ttttgcgatc ctggtcgaag agggcgggct
     3901 cgacatggcc aagcgcctgg ccgacaccgc gatgggcacg caggacgcgc tgctgcgcgg
     3961 ggcggcgctg ggctccgtca ccacgtccgg caaggaagag atcgcccgct gggtcctgac
     4021 cgatttcaac gatccgcggc tgcggcgcag cgagaagctg ggcctgatcc gcggcatcgt
     4081 cggcacccgc ggcacccgcg atttcggctt tgcctggctc aaggacaatt acgacgcgct
     4141 gagcgccggg ggcgggggga tcttcttcac ctccggcctg ccgaacctgg tcggcggcta
     4201 ttgttcggtc gccaaggccg acgagatcgc ggcgttcctg cggccgaagc tggccggcaa
     4261 gaccggcgcg ctttcgctgg aacggacgat cgaacgggtg cgctcgtgcg gcgtgctcaa
     4321 ggatgcgcgc ggcgccgagc tctcggccgc tttcggcaag gtcaagtaga ggcctgggtc
     4381 agctaagcgg caggacaagc gtggcggtca gccccgccac gctgccgtcc gcgccggggc
     4441 ggttggtcag cgtcaacgtg ccgccgtgct gttcggcaat cgcgcgggcc agggtcaggc
     4501 ccagcccggc cccgccggtg ccgctgttgc gcgacggttc gccccgggtg aagggctgca
     4561 tcatcgcctc gatccggtcc tcgggaatgc cggggccgtc gtcttcgatc cggatcaccg
     4621 ccgcaccatg ttcggccgtc agcgcgatcc gtgccgcgcc gccatagcgc agcgcgttgg
     4681 cagccaggtt gcgcaccgcg cgccgcagcc aggtggcgcg cagcggcagg accatccgcg
     4741 ccgcgggcgt gaagaccacc ggctcgccca gatcctcgaa ctcctcggcc acttgtccga
     4801 ccagcgcgtt gagctcggtc ggctccagcg ggtcgcttgg ccggccgacc cgggccaggc
     4861 tcaggatgtc gtccaggctg cgggtgatgt cctcgatcgt cgcggccatc cgggcgcgtt
     4921 cggtcggatc ctcgacgctt tcgatccgca cacgcagcgc tgccagcggg gtcttcagat
     4981 cgtggccgat cgcccccagc atcacgtcct tttcgtcgag cagggcggcg atccggtctt
     5041 ccatggcgtt gtgcgccagg atcagccggc gcatgtcgtc ggggccggag ggttccagct
     5101 gtccgtgggt gtcgcggtgg gcggcaaagc gttccagccg ggtggtcagc gccgccagcg
     5161 gccgggtgat gcggccgacg atcagcgcga tcgcgccaac catcagggca tagagaagca
     5221 gggtctggcc cagcagggtg aagatcagcg ccggttcgcc cttgggcacc ggcacccgca
     5281 cggtcagcca gcgctttgca gcgggcagct cgaccgcggc ggcaagcagg cgctggggcc
     5341 gctccggatc gcggcccagc agccgggcgc gccgttccag ccggcgcagc aggcgcggat
     5401 cgtcggacag cgggcgttcc accaccacca cttcgcccag gtccagcccc tggccttcga
     5461 tgatctcgcg caggtcttcc tcggcgcgcg agtggcggcg gccggtgcca aggtcgggcc
     5521 gggcgctgcc ggcctcgacc cccagctgcc ggtcaccctc ggggcgcggc gggcgcggat
     5581 cggggttgcc acgcgggggc gtgccgatca gcccttcctc gcgggcggcg ccgaacaggc
     5641 gcagcgcggc ggtgtggatc agcgcggctt cgcgccggtc catctgcgcg cggtacagca
     5701 ggacggcgct gatcccctgg gccagcagca gcgccagtgc gacggccaag agcagctgcc
     5761 cctgcaggct gcgcggccac aaaagccccc gaagccgctg catcattccc tcgccgcggg
     5821 ttcgcggcgg acgtcggcgg cgagcatgta gccgccgccc cagacggtct ggatcagcag
     5881 cgggttgcgg ctgtcgacct cgatcttgcg gcgcaggcgg ctgacctggt tgtcgaccgc
     5941 gcggtcgaac aggtgggcct cgcggccctg gaccatgtcg agcaggcggt cgcggtcaag
     6001 cacctggcgg ggatgctcca gaaaggccat cagcagccgg aattcgaccg aggaaatcgc
     6061 caccaccgcg ccttcgggat cggtcagctt gcgccgcagc ggatcgagcc gccagccgtc
     6121 gaacagcagg tgctcctcgt ccgtctgggc cggcgcgccc ttgctcgcgc ggcgcagcac
     6181 cgaacggatc cgggcaacca gctcgcgcgg ttcgaacggc ttggtgacat agtcatcggc
     6241 gccgatttcc agcccgacga tccggtcggt cgcctcggtc cgggcggtca gcaggatggt
     6301 cggcacggcc tgggtttcgg ccaggtggcg gcacagcgag agcccgtcct ctcccggcat
     6361 catgatgtcg agcaggacca gatcgggcgg atcgtcgcgc agccgggtcc gcgcctcggc
     6421 cgcgctggcg gcctggtgca cgacaaaccc ctggcgagcg agatactcgg ccaggggttc
     6481 gcgcagggcg gcttcgtcat cgacgaggag caggcgggtt gcgtcgctct ggtccatggg
     6541 tgacggggcc gcggtgtgga tcagttggcc gacggcggcg gcggcatatc gccgtgcgcg
     6601 ccgtgatcgc cgtgaccgcc ggggccgcgc tggccgcgct tgcccatccg ctcgcgcatc
     6661 tgggcgtggt gggccttgcg ctcttcgggg gtcagcttgc cgtccttgtt gctgtcggcg
     6721 gtgtcgaaca tcttgccgcg ggcggcctgg aactcctcgc ggctgaccgc gccgtccttg
     6781 ttggcatcgg ccatgcgcag catcatgccg gccatccggc cttcgccgcg cttgccgccc
     6841 atgcggcctt caccgcgctt gccgcgctca ccgcgcttct cgcccatggc ctggtgggcg
     6901 gcgtcgaact cggcccggct cagcgaaccg tccttgttgg tgtccatcgc atcgaacttc
     6961 ttgccgtgct cggcggcgcg gtcggcgcgg tccagcttgc cgtccttgtt ggtgtccatc
     7021 tggtcgaact gggtcccggc ctgggccatc atctcggccc gggtgacggt cttgccgccc
     7081 agcggatcgg catcggcggg gccggactgg gccaccgcgg ccgtggccag gccggtggac
     7141 agcagggcga aagcaatcag cgaggtcttc atggtgcgaa ctccttgtcg caggaaacgg
     7201 agagaagcca ggggcattcc gggagggggc agggggctgc ccttggcttc ctgtgatccg
     7261 ttctaggacc aagctgtcgc gcgagtgtgc cggttggcaa gcactttgta tcaatttgtc
     7321 gcaggatcgc cg
//
LOCUS       DASUDV010000113         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_533776,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000113 DASUDV010000000
VERSION     DASUDV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533776"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1158
                     /locus_tag="VFV30_02475"
     CDS             151..1158
                     /locus_tag="VFV30_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX4846984.1"
                     /translation="MAEMLPQSMRAVHFDAPGGPDVLKPADLPVPQPGPGEVLIRVAF
                     AGVNRPDVIQRQGFYPPPPGASPLLGLEVSGEIAALGEGVDAALLGQKVCALTPGGGY
                     AEYCRVPAGHCLPVPEGLPMDQAAALPETLFTVWHNVFERAYARDGETILVHGGTSGI
                     GSMAIMLGKAFGLTVIVTCGGADKCAAALEIGADHAIDYKAQDFVEEVARITGGKGVE
                     IVLDMVAGDYVARNLKCLADDGRHVTIAVQGGLQATLNMAEVMRRRLHLTGSTLRPRS
                     DAFKTLLAQEIAQLAWPLVESGELRPVMDRAFPLAEAAAAHARMEAGSHIGKIVLAVA
                     P"
     gene            complement(1168..1875)
                     /locus_tag="VFV30_02480"
     CDS             complement(1168..1875)
                     /locus_tag="VFV30_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846985.1"
                     /translation="MASRLRNGLVGLALLGLILAVGWCRSAPDTVGGSASLALSAPAV
                     QSRIAELAACPSPLERLGIERIHPKVMVGGDEIDLVIPSHGGGEGSMVRFHLDPVPGS
                     GGSQIRLRWQVRLAGNAKELDLGEDRLLNPANLSRELDSVVESHIAYYVNLMERGAAP
                     VSNRQRADKARNCRKAGRIIDAVAVVTNPALRQTIERQRRRDALGWLFKDDYQLRTGS
                     APGAYWEAEADFGPTGF"
     gene            complement(1885..2631)
                     /locus_tag="VFV30_02485"
     CDS             complement(1885..2631)
                     /locus_tag="VFV30_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846986.1"
                     /translation="MLHRVLFAALASLLLAACGRGSTDFDVLVERPPEKVRTALSGIA
                     IDQEIGALIPGLRLVRTEPAANEVLYTVPGDGSFEAAVKLTFEPSTDGKATMISVAVD
                     VPSTKVDFEGKAFVLSESKVETAIELILRTAAEKLKNGESIEAERAQFSQMLTILAVT
                     TDSSKLRLAQDIGKSPDWYMGGMGSMGDFDAGYGSAARGEDPAAGAREEEYKQREAER
                     AEREKAEEASSAMDSARGDSAAGDYAGPEE"
     gene            2789..>3313
                     /locus_tag="VFV30_02490"
     CDS             2789..>3313
                     /locus_tag="VFV30_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HEX4846987.1"
                     /translation="MTKPTPLMPHATASWLVDNTALSFEQIAEFCGLHILEVQAMADD
                     LASSKYTGRDPVRSGELTMAEIEKGQADPNYALVMQKAPVTVSRTKGPRYTPVSKRQD
                     KPDGIAWILRNHPEISDAQIGKLIGTTRNTIAAIRDRSHWNIANIQPKDPVTLGLCSQ
                     RELDAIVAKAAKKAG"
BASE COUNT          533 a         1129 c         1104 g          547 t
ORIGIN      
        1 gctccttcgc cagcatgctg gcggcatcgc ctttggcccg gggcaggtca tcgtccattg
       61 ccgggcagaa tcggacggcg aacgcttgct gtcaacacgc cttgaagcta gggcgctggg
      121 cgtggctggg gggcaacagg ggattggcaa atggctgaaa tgctgcctca atcaatgcgc
      181 gccgtgcact tcgatgcgcc gggcgggccc gatgttctga aaccggccga cctgccggtg
      241 ccgcagccgg ggccgggcga ggtcctgatc cgggtggcct ttgccggagt caaccggccc
      301 gacgtgatcc agcgccaggg cttctatccg cccccgccgg gcgcttcgcc gctgctgggg
      361 ctggaggttt cgggcgagat cgcggcgctg ggcgagggcg tcgatgccgc gctgctgggc
      421 cagaaggtct gcgcgctgac cccgggcggc ggctatgccg agtactgccg ggttccggcc
      481 gggcattgcc tgccggttcc ggaaggactg ccgatggatc aggccgcggc cctgcccgaa
      541 acgctgttca ccgtctggca caacgtgttc gagcgcgcct atgcccgcga tggcgagaca
      601 atcctggtcc acggcgggac cagcggcatc ggctcgatgg cgatcatgct gggcaaggcc
      661 tttgggttga ccgtgatcgt cacctgcggc ggggccgaca agtgcgccgc cgcgctggag
      721 atcggcgcgg atcacgcgat tgactacaag gcccaggatt ttgtcgagga agtggcccgg
      781 atcaccggcg gcaagggggt ggagatcgtg ctcgacatgg tcgcgggcga ctatgtggcg
      841 cgcaacctca agtgcctggc cgatgacggc cgccacgtga ccatcgcggt tcagggcgga
      901 ttgcaggcca cgctcaacat ggccgaagtg atgcggcggc gcctgcacct gaccggatcg
      961 accctgcgcc cgcgttcgga cgcgttcaag acgctgctgg cccaggaaat cgcccagctg
     1021 gcctggccgc tggtcgagag cggagagctg cgcccggtga tggaccgcgc ctttccgctg
     1081 gccgaggccg cggcggccca tgcccggatg gaagcgggca gccacatcgg caagatcgtg
     1141 ctggcggtgg ccccgtaacg ggtcggacta gaagccggtc gggccgaagt ccgcctcggc
     1201 ttcccaatag gcccccggcg cgctccccgt ccgcagctgg taatcgtcct tgaacagcca
     1261 gccgagcgcg tcgcggcggc gctggcgttc gattgtctgg cgcagggccg ggttggtcac
     1321 cacggcaacc gcatcgatga tccgcccggc cttgcggcaa ttgcgtgcct tgtccgcccg
     1381 ttgccggttt gagaccggtg cagcgcctcg ctccatcagg ttgacgtaat aggcgatatg
     1441 gctctccacc acgctatcca gctcgcgcga gagattggcg gggttgagca agcggtcttc
     1501 gccgagatcg agttccttcg cgttcccggc cagacggacc tgccagcgca gccggatttg
     1561 gctgccgccg ctgccgggaa ccggatcgag atggaagcgg accatgctgc cctcgccacc
     1621 cccgtgggac ggaatgacga ggtcgatctc gtcccctccc accatcacct tggggtggat
     1681 tcgctcaatt ccgagccgtt ccagcggtga cgggcaggcg gccagttcgg caatgcggct
     1741 ctgtactgcg ggggcggaca gcgccaggct ggcggagccg cccaccgtgt ccggagcgga
     1801 gcggcaccag cccaccgcca ggatcagccc cagcagcgct agcccgacca acccgtttcg
     1861 cagcctgctc gccatcggca ggcgctattc ttccggcccc gcatagtcgc cagccgccga
     1921 atcgccgcgc gcgctgtcca tcgcggacga ggcttcctcg gccttctcgc gctcggcgcg
     1981 ttcggcttcg cgctgcttgt attcctcttc ccgcgccccg gccgccggat cttcgccgcg
     2041 ggccgcgctg ccatagccgg catcgaaatc gcccatgctg cccatgccgc ccatgtacca
     2101 gtcgggcgac ttgccgatat cctgcgccag ccggagcttg gacgaatcgg tggttaccgc
     2161 caggatggtc agcatctgcg aaaactgggc gcgctcggct tcgatcgatt ccccgttctt
     2221 cagcttttcc gccgccgtgc gcaggatcag ctcgatcgcc gtttcgactt tcgattcgct
     2281 caggacaaag gccttgccct cgaagtcgac cttggtgctc ggcacgtcga ccgcgaccga
     2341 gatcatcgtg gccttgccat cggtcgaggg ctcgaaggtc agcttgaccg cggcttcgaa
     2401 cgagccgtcg ccgggaacgg tgtagagcac ctcgttggcg gccggctcgg tccgcaccag
     2461 ccggaggccc ggaatcagcg cgccgatttc ctggtcgatc gcgatgcccg acagggcggt
     2521 gcggaccttt tccggcgggc gttccaccaa aacatcgaaa tcggtggagc cgcggccaca
     2581 ggcggccagc agcaggctgg ccagggcggc gaacagaacg cggtgcaaca tggtatcccc
     2641 cctcgggtgt ctcatccggg gcaacttata gttacgattc actaacatgc ggtttgcatg
     2701 cttgccccgc gcccggaaac ggcctagatg ctggccaaac ggccgctccg cgaggggcgg
     2761 cccttctatt tagagccgga gaaaccccgt gaccaagccg accccgctta tgccgcacgc
     2821 caccgcttcg tggctggtcg acaacaccgc gctgtcgttt gaacagatcg ccgaattctg
     2881 cggcctgcac attctcgaag tccaggccat ggccgatgac ctcgccagtt cgaagtacac
     2941 cggccgcgat ccggtccgct cgggcgagct gaccatggcc gagatcgaaa agggccaggc
     3001 cgatcccaac tatgccctgg tgatgcagaa ggcgccggtc acggtcagcc gcaccaaggg
     3061 cccgcgctac accccggtgt cgaagcgtca ggacaagccc gatggcatcg cctggatcct
     3121 gcgcaaccac ccggaaatct cggacgcgca gatcggcaag ctgattggca cgacccgcaa
     3181 caccatcgcc gcgatccgcg atcgcagcca ctggaacatc gccaacatcc agccgaagga
     3241 cccggtcacc ctgggcctgt gcagccagcg cgagctggac gcgatcgtgg ccaaggcggc
     3301 gaagaaggcc ggc
//
LOCUS       DASUDV010000114         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1417173,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000114 DASUDV010000000
VERSION     DASUDV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417173"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFV30_02495"
     CDS             <1..763
                     /locus_tag="VFV30_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX4846988.1"
                     /translation="MPVVVSMLNSYSGWAAAAMGFTLQNTAMIITGALVGSSGAILSY
                     IMCRAMNRSFISVIAGGFGGDAVAAGGAAAIDRPFKRGSAEDAAYMLGEAEKVIIIPG
                     YGMAVSQAQHALREMGDLLKAKGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFEL
                     EDINSEFATADVAFIIGANDVVNPAAKTDKSSPIYGMPVFDVDKAKQVFFIKRSMGGV
                     GYAGVDNDVFYMDQTLMLLADAKKMVEDINKGLAH"
     gene            794..2176
                     /locus_tag="VFV30_02500"
     CDS             794..2176
                     /locus_tag="VFV30_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX4846989.1"
                     /translation="MSETAGHHRLERALSPLEVMLLTLSALSPALSVFVGGNAVLHLA
                     GTGAALGFILGGLIAALFALLFAELASGKAGAGGTYAGLNAFLGPRWTFPYVMVRLVI
                     VFPMTAFISLGFGTFVHDLVPAISTNLGALLAIAAAAGVAVLQVKRGSQVIAVFLVIE
                     ALALAVLTWVALTHGVRSLGEVLANPVVLGKDGALAAVEPGMMALGVVSGASMASGAN
                     WATYFAENMDDARRRIGRVVAWAGLIAALTIAVPVVLVVLAIDNVPAVLAHPAPMAEF
                     LRRSAGPTTATLVSIGVAAAVFNAVIAAIMGLSRQLFAIARDGVFPAWIGEPLARLHP
                     RYRSPAWAVLALSLLGALCLLLGERRLLLIISGEFAEYFLISAAILLARRGTGPAAHF
                     RAALHPLLPLGGIAMGVVILLANWNDAETARLSMLFLLGIFVTAFIWHEARIRQGRPA
                     VVLKGSDIDD"
     gene            2169..2486
                     /locus_tag="VFV30_02505"
     CDS             2169..2486
                     /locus_tag="VFV30_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4846990.1"
                     /translation="MIDRSILLPALIALAACSPSKLAYGTVKSALSDAGLSEANAACM
                     AERMTDRLTIGQLRKLQKLKGERRTLGDYVAAVRRVGDAEALSVTASSAALCATGLAA
                     EKR"
BASE COUNT          403 a          807 c          931 g          510 t
ORIGIN      
        1 catgccggtc gtggtgtcga tgctgaacag ctattccggc tgggcggccg cggcgatggg
       61 cttcacgctc cagaacacgg cgatgatcat caccggggcg ctggtgggct cgtcgggcgc
      121 gatcctcagc tacatcatgt gccgggcgat gaaccgcagc ttcatctcgg tcatcgccgg
      181 cggcttcggc ggcgatgcgg tcgcggccgg cggggcggcg gcgatcgacc ggccgttcaa
      241 gcgcggctcg gccgaggacg cagcctatat gttgggcgag gccgagaagg tcatcatcat
      301 cccgggctat ggcatggcag tcagccaggc gcagcacgcg ctgcgcgaaa tgggtgacct
      361 gctgaaggcc aagggcgtcg aggtgaagta cgcgatccac cccgtcgcgg ggcgcatgcc
      421 cgggcacatg aacgtgttgc tggcagaagc caacgtgccg tacgacgaag tgttcgaact
      481 ggaagacatc aactccgaat tcgctaccgc cgacgtcgcc ttcatcatcg gtgccaacga
      541 cgtggtgaac cccgcggcca agaccgacaa gtcatcgccg atctatggca tgccggtgtt
      601 cgatgtggac aaggccaagc aggtgttctt catcaagcgc tcgatgggcg gcgtgggtta
      661 tgccggggtc gataacgacg tgttctacat ggaccagacc ctgatgctgc tggccgatgc
      721 gaagaagatg gtcgaagaca tcaacaaggg gctggcgcac taggccacag gcgacaaggc
      781 gggggatccg gggatgagcg aaacggcggg ccatcaccgg ctggaacggg cgctttcgcc
      841 gcttgaagtc atgctgctga cgctatcggc gctgtcgccc gcgctgtcgg tcttcgttgg
      901 tggcaacgcc gtgctgcatc tggccggaac cggcgcggcg ctgggtttca tcctgggcgg
      961 gctgatcgcc gcgctgttcg cactgctctt tgccgaactg gcatcgggca aggccggtgc
     1021 agggggcacc tatgccgggc tcaatgcttt cctggggccg cgctggacct ttccctatgt
     1081 gatggtgcgg ctggtcatcg tgttcccgat gactgccttc atctccctgg gtttcggcac
     1141 tttcgtccat gatctggtcc cggccatttc gaccaatctg ggggccctgc tggccatcgc
     1201 ggctgcggcc ggggtggcgg tgctgcaagt caagcgcgga tcgcaggtca tcgcggtgtt
     1261 cctggtaatc gaggcactgg cgcttgccgt gctgacctgg gttgcgctga ctcacggcgt
     1321 gcgctcgctg ggcgaggtgc tggccaaccc ggtggtgctg ggcaaggatg gcgcgctggc
     1381 ggcggtggag ccggggatga tggcgctggg cgtggtttcg ggcgcctcga tggcctcggg
     1441 ggcaaactgg gccacctatt ttgccgagaa catggacgat gcccgccgcc ggatcgggcg
     1501 ggtggtcgcc tgggccgggc tgatcgccgc gctgaccatc gccgtgccgg tggtgctggt
     1561 ggtgctggcg atcgacaatg tgcccgcggt cctcgctcat cccgcgccga tggcggaatt
     1621 cctgcgtcgc agcgccgggc caaccacggc gacgctggtt tcgatcgggg ttgcggcggc
     1681 ggtgttcaac gcggtgatcg cggcaatcat gggcctctcg cgccagcttt tcgcgatcgc
     1741 ccgcgacggg gttttcccgg cctggatcgg cgagccactg gcgcggctgc atccgcgcta
     1801 tcgttcgccg gcctgggctg tgctggcgct ttcgctgctg ggcgcgctgt gcctgctctt
     1861 gggcgaacgc cgcctcctgc tgatcatttc aggcgaattt gccgaatatt tcctgatttc
     1921 ggctgcaatc ctgctggccc ggcgcggcac cgggccggcg gcacatttcc gcgccgcctt
     1981 gcacccgctg ctgccgctgg gcgggatcgc gatgggggtg gtaattctgc tggccaactg
     2041 gaacgatgcc gaaaccgcac ggctttcgat gctgttcctg ctggggatct tcgtcaccgc
     2101 gttcatctgg catgaagcca ggatcaggca aggcaggccg gctgttgtcc tgaagggttc
     2161 cgatatcgat gattgaccgc tcgatcctgc tgcccgcgct gatcgcgctt gccgcctgca
     2221 gcccgtcgaa gctggcctat ggcacggtca agagcgcgct gagcgatgcc ggcctttccg
     2281 aagccaatgc cgcctgcatg gccgagagga tgaccgaccg gctgaccatc ggccagctgc
     2341 gcaagctgca gaagctcaag ggcgaacgcc gcaccctggg cgactatgtc gccgcggtgc
     2401 gccgggttgg cgatgcggag gcgctttccg tgactgccag cagcgcggcg ctgtgcgcaa
     2461 ccgggctggc ggctgaaaaa agataggatg aaagtcgtgc cgcgacagaa agtgatcatc
     2521 tacccggcca gcttggcagg cctgcttttc gctacgccag ccagcgccgc aatcgatgat
     2581 ttgcagatcg attgggttgt tccggcagac ggcttcgttc gatcggatga agacgcggca
     2641 aagggtattc c
//
LOCUS       DASUDV010000115         6718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1903277,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000115 DASUDV010000000
VERSION     DASUDV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903277"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VFV30_02510"
     CDS             complement(<1..98)
                     /locus_tag="VFV30_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4846991.1"
                     /translation="MTQIRPFTLAVPQSDLDDLYRRLDQTRWPEKEA"
     gene            184..1356
                     /locus_tag="VFV30_02515"
     CDS             184..1356
                     /locus_tag="VFV30_02515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4846992.1"
                     /translation="MVQPQLAGIKVVDLTTVVFGPYCTQILADLGAEVIKVETPGHGD
                     AFRWSAQAAHTPGMAPGFMAVNRGKRSIALDLKAEDDRAVMLDLLREADLFVVNVRGK
                     ALERLGLDWDSLRALNPRLIYVHCVGFGQDGPYADLQAYDDVIQAATGTATLLPRVDG
                     NPRPRYLPSLIADKVAGLHAAYGALAALYRREQSGTGQRVEVPMFEVFSSFMLLEHLG
                     GQTFDPPVGPVGYARQVDPVRQPFPTADGAIAIVMYNYDAWDRIFAVLEDPDFIAQER
                     FTVKGGRAIHQDALYTRIAELTPNFTTAELQRRCDAAQLPAQAVRDLSEVIADPHLNA
                     VGFFTRREHPSEGAYFEQAQPVRFSDGAHSPARMPPRLDEDGAAIRAERASRPSPP"
     gene            complement(1334..1753)
                     /locus_tag="VFV30_02520"
     CDS             complement(1334..1753)
                     /locus_tag="VFV30_02520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4846993.1"
                     /translation="MADNSLYEEMGLTRIVELDPAGRAVLEYEARLSQCHSGGVVQGG
                     FVTGWIDAAMAHAAIALGGDDLVPMTLELKVSFFAPARPGPVLAEGWVVKHGKRTAFY
                     EGALKDGQGNLLAKATSTLALIPRGKVEAASKAATAG"
     gene            complement(1753..2973)
                     /locus_tag="VFV30_02525"
     CDS             complement(1753..2973)
                     /locus_tag="VFV30_02525"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4846994.1"
                     /translation="MAQTQSAAGSFDPARLARLDAFIKERYLDTGLLPHAQVLVAREG
                     EIAHFSSQGPAREGGKPIDESSIFRIASMTKPVTSLAFMQLLEQGKVALETPVHAVLP
                     EFKGQGVYAGGGGGVPFLTRPCDRVMTMQDLLRHTSGLTYSFQNRSNIDLAHREGKLE
                     NWHGNLTLDELVGALGKLPLEFSPGTAWNYSVSTDVLGAVVQRLSGQSLDAYFAEHIF
                     QPLGMHDTGFMVPADKIDWLTDCYTFVPGKGRVMYDRGAESAWSRMPRQLSGGGGLVS
                     TALDYHRFCTMLLNGGALEGERVIGRKTLELMTLNHLPGGADIASMSKSLFSEEAFGG
                     VGFGLGFAVNLCPARSMLPGSVGEYNWGGMFSTYFFVDPVEKLHMVFMTQLSPSSTYP
                     VRRELKTLIYSALA"
     gene            3068..3883
                     /locus_tag="VFV30_02530"
     CDS             3068..3883
                     /locus_tag="VFV30_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX4846995.1"
                     /translation="MSVSDLPIKVAALYRFTPLDGLPELRAQLLDLCRDEGIRGTLLL
                     AREGINGTIAGPDAALDRVIAHIRALPGCAEIEVKWSRAPALPFQRMKVRLKAEIVTM
                     GQPDIDPLQGTGHYLDPAEWNALIADPATLVIDTRNAYEIEAGTFARAVDPGTRSFRE
                     FPDWFRANRAALLGEGKRKVAMFCTGGIRCEKSTAFLKAEGVEEVYHLKGGILKYLED
                     MPEEQSLWQGECFVFDERVTVGHGLAPGTFIMCPDCGRPLRQGQACGDCGAGR"
     gene            4062..5738
                     /gene="leuA"
                     /locus_tag="VFV30_02535"
     CDS             4062..5738
                     /gene="leuA"
                     /locus_tag="VFV30_02535"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX4846996.1"
                     /translation="MTMLKNPGVKYRAFPPINLPDRQWPSRQITKAPRWLSTDLRDGN
                     QALIDPMGAEKKMRFFDLLLKVGCKEIEVGFPASGATDFDFISGLVRQDKIPEDVAVQ
                     VLTQSRRDLIETSFASLEGAKTAIVHLYNAVSPVWRKVVFGMTKDQIKEIAIEGAKVL
                     RDQAAKYHGTDWVFEYSPETFSTAEVEYSVEVCAAVMDVLQPTPEKPIIFNLPATVEC
                     ATPNVYADQVELFGRLIPNRDSVVISLHTHNDRGTGIAAAELGLMAGGDRVEGCLFGN
                     GERTSNCDIVTVALNMYTQGVDPELDFSNIDEIAKTVEYCNQLPIHPRHPYVGELVYT
                     SFSGSHQDAIKKGFAAREKQNDELWEMPYLPIDPADLGRDYEAVIRVNSQSGKGGFAW
                     VLEQDQGLKLPKKMQAHFSKHVQELADEVGRELFADDIWTVFQKTYHLNTPQHFTLVD
                     YEETKAADGTRLFAGKIAVDDQEQSVSGRGNGLISSVVATIGETFGVQLEVKDYAEHA
                     MSAGSDARAAAYVECTGPDGKTVWGVGIDEDVATASVRAVLSAANAAAKA"
     gene            complement(5735..>6718)
                     /locus_tag="VFV30_02540"
     CDS             complement(5735..>6718)
                     /locus_tag="VFV30_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HEX4846997.1"
                     /translation="PTPQTDRDAVVRVTACSICGSDLHIYHGHGFSEDTGFCVGHEAV
                     GEVVEVGRGVQRLKVGDTVMLPAAVGCGQCRSCLAGVVNTCEYGMSGCYGLSAKLQGS
                     QAEAVRVPAADMNAVPVPEGVSMDQALMMTDALATAWFGARNADIRPGSSVAVIGLGP
                     IGLMAVDSAFVMGAHVVYAVDPIPKRRALAEAAGAIALHPDEALERIKQDTKGRKLDC
                     VVEVVGSDATVDFALRLVRPRGTVSVIGVQQSRRFAFPLERAFAAGLTFRVGTCSVPE
                     ELPALFPLVQSGRLRPEKYVSHRLPLSEGAEAYRLFEAREAGALKMVLTPD"
BASE COUNT         1162 a         2302 c         2192 g         1062 t
ORIGIN      
        1 gcctccttct cgggccagcg ggtctggtcg agccggcgat agagatcgtc cagatcggac
       61 tgcgggacgg cgagcgtaaa ggggcgaatc tgagtcatgg cggcaggcta ggccaagttc
      121 ggcgttgtgt taaccgttcg attaaagata atcggccgca gaggaaacgg gagagcatcc
      181 gatatggtcc aaccgcagct agccgggatc aaggtggtag acctgaccac cgtggtcttt
      241 ggcccctatt gcacccagat cctggccgat ctgggggccg aggtgatcaa ggttgaaacg
      301 ccgggtcacg gcgatgcctt tcgctggtcg gcccaggcgg cccatacccc gggcatggca
      361 ccggggttca tggccgtgaa ccgcggcaaa cgctcgatcg cactggacct caaggccgag
      421 gacgatcgcg cggtcatgct cgatctgctg cgcgaggcgg acctgttcgt ggtcaatgtc
      481 cgcggcaagg cgctggaacg gctcgggctg gactgggaca gcctgcgcgc gctcaacccc
      541 cggctgatct atgtccactg cgtcggcttt ggccaggacg gcccctatgc cgatctgcag
      601 gcctatgacg acgtgatcca ggccgcgacc gggacggcca ccctgctccc gcgcgtcgat
      661 ggcaatcccc gcccgcgcta tctgccctcg ctgattgccg acaaggtcgc cgggctgcac
      721 gccgcctatg gcgcgctggc ggcgctctac cggcgcgaac agagcggcac gggccagcgg
      781 gtcgaagtgc cgatgttcga ggtcttttcc agcttcatgc tgctggagca tctgggcggg
      841 cagaccttcg atccgccggt cggcccggtc ggctatgccc gccaggtcga tccggtgcgc
      901 cagcccttcc ccaccgccga cggcgcgatc gcgatcgtga tgtacaacta tgacgcctgg
      961 gaccggatct ttgccgtgct ggaagacccg gacttcatcg cgcaggagcg tttcaccgtg
     1021 aagggcggac gggcgatcca ccaggacgcg ctctacaccc ggatcgcgga actgaccccc
     1081 aacttcacca cggccgagct gcagcgccgc tgcgacgcgg cgcagctgcc ggcccaggcg
     1141 gtgcgcgacc tctccgaagt gattgccgat ccgcatctga acgcggtcgg cttcttcacc
     1201 cggcgcgagc atcccagcga gggcgcctat ttcgagcagg cccagccggt gcgattctca
     1261 gacggcgcgc acagccccgc ccggatgccg ccgcggctgg acgaggacgg cgccgcgatc
     1321 cgcgccgaac gggctagccg gccgtcgccg ccttagacgc ggcctccacc ttgccgcggg
     1381 ggatcagcgc cagggtgctg gtcgccttgg ccagcaggtt gccctgcccg tccttcagcg
     1441 cgccttcgta aaaggcggtg cgcttgccgt gcttgaccac ccaaccttcg gccaggaccg
     1501 ggcccggccg ggccggagcg aagaagctga ccttcagttc cagcgtcatc gggaccagat
     1561 cgtccccgcc cagcgcgatc gcggcgtggg ccatggcggc gtcgatccag ccggtcacga
     1621 acccgccctg caccacgccg ccggaatggc attgcgagag ccgcgcttcg tattccagca
     1681 ccgcgcggcc ggccggatcg agctcgacga tccgggtcag gcccatctcc tcgtacagcg
     1741 aattgtccgc cattacgcca gcgccgaata gatcagggtc ttgagctcgc gccggaccgg
     1801 ataggtgctg ctgggcgaaa gctgggtcat gaacaccatg tgcagctttt cgaccggatc
     1861 gacgaagaag taggtcgaga acattccgcc ccagttgtac tcgccgaccg agcccggcag
     1921 catcgagcgc gcggggcaga ggttgaccgc aaaaccgagg ccaaagccga ccccgccgaa
     1981 cgcctcttcg ctgaacaggc ttttcgacat cgaggcgatg tccgcgccgc cgggcaagtg
     2041 gttcagcgtc atcagctcca gcgtcttgcg gccgatcacg cgttcgccct ccagcgcgcc
     2101 gccgttcagc agcatggtgc agaaccggtg atagtcgagc gcggtcgaga ccagcccgcc
     2161 gccgcccgaa agctggcgcg gcatccggct ccaggcgctt tccgcgccgc gatcgtacat
     2221 cacccggccc ttgccgggga cgaaagtgta gcaatcggtc aaccagtcaa tcttgtccgc
     2281 cggcaccata aagccggtgt cgtgcatccc cagcggctgg aagatgtgct cggcaaaata
     2341 ggcgtccagg ctctgccccg aaagccgctg caccaccgcg cccaggacat cggtggagac
     2401 cgaatagttc cacgccgtac cgggcgagaa ttccagcggc agcttgccca gcgcgccgac
     2461 cagttcgtcc agcgtcaggt tgccgtgcca gttctccagc ttgccctcgc ggtgggccag
     2521 gtcgatgttg ctgcggttct ggaacgaata ggtcagcccg ctggtgtggc gcagcaggtc
     2581 ctgcatggtc atcacccggt cgcacgggcg ggtcaggaac ggcaccccgc ccccgccgcc
     2641 ggcatagacg ccctgcccct tgaattcggg cagcacggca tggaccgggg tttccagcgc
     2701 aaccttgccc tgttccagca gctgcatgaa ggccagcgag gtgaccggct tggtcatgct
     2761 ggcgatgcga aagatcgagc tttcgtcgat cggtttgccc ccttcgcggg ccggtccctg
     2821 cgaggagaag tgcgcgatct cgccctcgcg cgcgaccagc acctgggcgt gcggcagcaa
     2881 gccggtatcg agatatcgtt ccttgatgaa ggcatcgagc cgggcaaggc gggcaggatc
     2941 gaacgatcca gcggcggatt gggtctgggc catcggggct ccgtgatgat cggttgcagt
     3001 ttcgcaccga ctattgcggg gcgaaccggg ctggtcaatg ccgccgtccc tggactaggg
     3061 ggcaagcgtg tccgtttccg atctcccgat caaggttgct gccctttacc ggttcacgcc
     3121 gctggacggc ctgccggagc tgcgcgcaca gctgctcgat ctgtgccggg acgaaggcat
     3181 ccgcggcacg ctgctgctgg cgcgcgaggg gatcaacggg accattgccg gccccgatgc
     3241 cgcgctggac cgggtgatcg cgcacatccg cgcccttccc ggctgcgcgg agatcgaggt
     3301 gaaatggtcc cgcgcccccg ccctgccgtt ccagcggatg aaagtgcggc tgaaggccga
     3361 gatcgtgacc atgggccagc cggacatcga tccgctgcaa ggcaccgggc attaccttga
     3421 cccggccgaa tggaacgccc tgatcgccga tcccgccacc ctggtgatcg acacccgcaa
     3481 cgcttacgag atcgaggccg gcacctttgc ccgggcagtc gatcccggca cgcggtcgtt
     3541 ccgcgagttt cccgactggt tccgcgccaa ccgcgccgcg ctgttgggcg aaggcaagcg
     3601 caaggtcgcg atgttctgca ccggcggcat ccgctgcgag aaatcgaccg ccttcctgaa
     3661 agccgagggc gtcgaggagg tttaccacct caagggcggc atcctcaaat acctggagga
     3721 catgcccgag gagcagagcc tgtggcaggg cgaatgcttc gtcttcgacg agcgggtgac
     3781 cgtgggccac gggctggccc cggggacttt catcatgtgc ccggactgcg gccgcccgct
     3841 gcgccagggc caggcctgcg gcgattgcgg cgcgggccgc taaacccctt tacagcgccc
     3901 atgcctgccg ccataggctt gccccatgat ccgcgcgatt ggccttaccc tgcgacttat
     3961 ccgcccttgg gcgtgagctg gcgtgcgcag aggcggcacg cagcgccgct caagggacct
     4021 gacaccgcca taccgtcgca ctgccaatca gagataccgt catgaccatg ctgaagaacc
     4081 cgggcgtcaa gtaccgcgcc tttccgccga tcaacctgcc cgaccgccaa tggccgagcc
     4141 gccagatcac caaggccccg cgctggcttt ccaccgacct gcgcgatggc aaccaggcgc
     4201 tgatcgatcc gatgggcgcg gaaaagaaga tgcggttctt cgacctgctg ctgaaagtcg
     4261 gctgcaagga gatcgaggtc ggcttccccg cctcgggcgc gaccgatttc gatttcatca
     4321 gcgggctggt gcgccaggac aagatccccg aggacgtcgc cgtccaggtc ttgacccagt
     4381 cgcgccgcga cctgatcgag accagctttg ccagcctgga aggcgcgaag accgcaatcg
     4441 tccacctcta taacgccgtc tcgccggtct ggcgcaaggt cgtgttcggc atgaccaagg
     4501 accagatcaa ggagatcgcg atcgagggcg ccaaggtgct gcgcgatcag gcggccaagt
     4561 atcacggcac cgactgggtg ttcgagtaca gccccgaaac cttctcgacc gccgaggtcg
     4621 aatactcggt cgaggtctgc gcggcggtga tggacgtgct gcagccgacg ccggaaaagc
     4681 cgatcatctt caacctgccc gccacggtcg aatgcgcgac gcccaacgtc tatgccgacc
     4741 aggtcgagct gttcgggcgg ctcatcccca accgtgacag cgtggtgatc agccttcaca
     4801 cccacaacga ccgcggcacc ggcatcgcgg cggccgaact gggcctgatg gccggcggcg
     4861 accgggtgga aggctgcctg ttcggcaacg gcgagcgcac cagcaactgc gatatcgtca
     4921 ctgtcgctct caacatgtac acgcaaggcg ttgatccgga gctggatttc tcgaacatcg
     4981 acgagatcgc caagacggtg gagtactgca accagctgcc gatccacccg cgccatccct
     5041 atgttggcga actggtctac acctcgttct ccggcagcca ccaggacgcg atcaagaagg
     5101 gcttcgccgc gcgcgagaag cagaacgacg agctgtggga aatgccctat ctgccgatcg
     5161 atccggccga ccttggccgc gattacgaag cggtgatccg ggtcaattcc caatccggca
     5221 agggcggttt cgcctgggtg ctggaacagg accagggcct gaagctgccc aagaagatgc
     5281 aggcgcattt ctccaagcac gtgcaggaac tggccgacga agtgggccgc gagctgtttg
     5341 ccgacgacat ctggaccgtg ttccagaaga cctatcacct caacaccccg cagcatttca
     5401 cgctggtgga ctatgaggaa accaaggccg ccgacggcac ccgcctgttc gccggcaaga
     5461 tcgcggtgga cgatcaggag cagtccgtct cggggcgcgg caacggcctg atcagctcgg
     5521 tcgtggcgac catcggcgaa acctttggcg tgcagcttga ggtcaaggac tacgccgaac
     5581 acgcgatgtc cgccgggtcg gacgcgcgcg cggcggccta tgtcgaatgc accggtccgg
     5641 acggcaagac cgtctggggc gtcggcatcg acgaggacgt cgccaccgcc tcggtccgcg
     5701 ccgtgctgag cgcggccaac gcggcggcga aggcctagtc cggggtcagc accatcttca
     5761 gcgcgccggc ttcgcgcgcc tcgaacaggc ggtaggcctc tgccccctcg ctgagcggca
     5821 ggcggtggct gacgtacttt tcggggcgca gccggcccga ctggaccagc gggaacagcg
     5881 cgggcagctc ttccgggacc gagcaggtgc cgacccggaa ggtcagcccg gcggcaaagg
     5941 cgcgttccag cgggaaggca aaccggcggc tctgctggac gccgatcacc gaaaccgtcc
     6001 cgcgcgggcg gaccagccgc agcgcgaaat cgacggtggc atcgctgccg accacttcga
     6061 ccacgcagtc gagcttgcgg cccttggtgt cctgcttgat ccgctccagc gcctcgtcgg
     6121 gatgcagcgc gatcgcgccc gcggcctcgg ccagcgcgcg gcgtttcggg atcggatcga
     6181 ccgcatagac cacgtgggcg cccatcacga aggcgctgtc gaccgccatc agcccgatcg
     6241 ggccaagccc gatcaccgct acgctggaac ccggccggat atcggcgttg cgcgcgccga
     6301 accaggcggt ggcgagcgcg tcggtcatca tcagcgcctg atccatggag acgccctcgg
     6361 gcaccggcac ggcgttcatg tcggccgccg gcacccgcac cgcttcggcc tgcgagccct
     6421 gcagcttggc ggagaggccg taacagccgc tcatcccgta ttcgcaggtg ttgaccacgc
     6481 ccgccaggca ggaccggcac tgcccgcagc ccacggccgc cggcagcatg acggtgtccc
     6541 ccaccttgag ccgctgcacg ccccgcccga cctcgaccac ttcgcccacc gcctcgtgcc
     6601 cgacgcagaa gccggtatcc tcggaaaagc cgtgaccgtg gtagatgtgc aggtccgatc
     6661 cgcagatcga gcaggccgtg accctgacca ccgcatcccg atcggtttgc ggggttgg
//
LOCUS       DASUDV010000116         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1748742,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000116 DASUDV010000000
VERSION     DASUDV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748742"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VFV30_02545"
     CDS             <1..132
                     /locus_tag="VFV30_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX4846998.1"
                     /translation="GILQGIKKRVIDGTASSAHAKAQSERVQPLVDRATEFAKKAGM"
     gene            complement(160..1392)
                     /locus_tag="VFV30_02550"
     CDS             complement(160..1392)
                     /locus_tag="VFV30_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4846999.1"
                     /translation="MSDKPRALDGIKVLDLSRVLAGPWSTQILADFGADVFKVEQPGR
                     GDDTRGWGPPFLKLPDGSDDPRESAYYLCANRNKRSAAIDIAKPEGAALIRRLAAQAD
                     VLVENFKVGGLAKYGLDYAAIRAVNPRIVYCSVTGFGQDGPYASRGGYDFLAQGMGGL
                     MSVTGEADGPPLKVGVAMADLATGMYATVSILAALRHAERTGEGQQIDISLLDTQIAM
                     LANQASSYLVGGVVPGRMGNRHPTVVPYTTFAVTDGTVIIAIGNDIQFRAFCAEMGVA
                     ELGSDPRFATASARVIHRDAIEEIMRDLVKGESRDGLIARLEAAGVPVGPVNTVEDVF
                     ADPFVAARQTVHRFVREDGVEIPTVAYPGKLSATPADYRRQPPRVGEQTRELLGEWLG
                     LDAAELAALEESGVVQQG"
     gene            complement(1425..>2991)
                     /locus_tag="VFV30_02555"
     CDS             complement(1425..>2991)
                     /locus_tag="VFV30_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4847000.1"
                     /translation="ISAFSNKAGTEAQWYLSARGERVCCVYENGVLKVVTNDDGSRSV
                     YVFSGTSFAAPQIAGAAALLRQAFPNLTATQVVDLLLRTARDAGDAGTDPLYGRGILD
                     IAAAFAPQGTTTLAGSPVAMPLGDSTGVTSAPMGDAGRPAKGIGVIVLDGYQRAYQID
                     LTAGFTSAQIQPRLGPALSRESRSVTIGGEDLTMAFTVDGNGKVARMPWSGQLRLSHR
                     DAEIARVLAARAVARIAPNSDIAFAFAQGSDGLVAQLQGRSQPAFLIARGPTDDVGFG
                     RDESFSLAWRRQAGGWGVTFGAEHGSAMTAAPVISAATNVARTRLDPADRLSVSFDRR
                     FGPVSATFGAGWLGESRTLLGARFHEGFGASGAESLFLDASGEWRPAPRWRLGAAWRS
                     GFTWPHTGGSIAPGSRLTTSAWAFDLGREGLFQRGDSLTFRLAQPLRVESGGLALNLP
                     VDYSYITLQPTFALVPLSLAPRGREIDAELIWRGRLLSGAALFSLFYRKDPGHYAAVR
                     DDQGVAVSWTKQF"
BASE COUNT          469 a         1067 c         1037 g          418 t
ORIGIN      
        1 gggatccttc agggcatcaa gaaacgggtg atcgacggca ccgccagctc ggcccacgcc
       61 aaggcccagt cggaacgggt ccagccgctg gtcgaccgcg cgacggagtt tgccaaaaag
      121 gcggggatgt aggcctcccc gccgggggaa gcccgttcct tacccctgct gcaccacgcc
      181 gctctcttcc agcgcggcga gttcggccgc gtccagcccc agccattcgc ccagcagctc
      241 gcgggtctgc tcgcccacgc gtggcggctg gcggcggtaa tcggccggag tggcggagag
      301 cttgccggga taggccacgg ttgggatttc caccccgtcc tcgcgcacga agcggtgcac
      361 ggtctggcgg gccgcgacga aggggtcggc gaagacatcc tccaccgtgt tgaccgggcc
      421 gaccgggacc ccggccgctt ccagccgcgc gatcaggccg tcgcggcttt cgcccttcac
      481 cagatcgcgc atgatctcct cgatcgcgtc gcggtggatc acccgggccg atgcggtggc
      541 aaagcgcgga tcgctgccca gctcggccac gcccatttcg gcgcagaagg cgcggaactg
      601 gatgtcgttg ccgatcgcga tgatcacggt cccgtcggtc acggcgaaag tggtataggg
      661 caccacggtg ggatggcggt tgcccatccg ccccggtacc accccgccga ccagatagct
      721 ggaggcctgg ttggccagca tcgcgatctg ggtgtcgagc agcgagatgt cgatctgctg
      781 cccttcgccg gtccgctcgg catggcgcag cgcggcgagg atcgagacgg tggcgtacat
      841 ccccgtcgcc agatcggcca tggccacgcc gaccttcagc ggcgggccgt ccgcctcgcc
      901 ggtcacgctc atcagcccgc ccatgccctg ggccaggaag tcatagccgc cgcggctggc
      961 atagggcccg tcctgcccga acccggtgac cgagcaatag acgatccgcg gattgaccgc
     1021 gcggatcgcg gcatagtcca gcccgtactt ggccagcccg ccgaccttga aattctccac
     1081 cagcacatcg gcctgggcgg ccaggcggcg gatcagcgcg gcgccttcgg gcttggcaat
     1141 atcgatcgcg gccgagcgct tgttgcggtt ggcgcagagg taataggcgc tttcgcgcgg
     1201 atcgtccgat ccgtccggca gcttcaggaa cggcgggccc cagccgcggg tgtcatcgcc
     1261 ccggccgggc tgttccacct tgaacacgtc ggcgccgaaa tcggccagga tctgcgttga
     1321 ccagggaccg gccagaaccc gcgagaggtc cagcaccttg atcccgtcga gggcacgggg
     1381 tttgtcgctc attccgctcc catgccgggg cggcgcgaac ctgtctagaa ctgcttggtc
     1441 cagctcaccg ccacgccctg atcgtcgcgg accgcggcat agtggcccgg gtccttgcgg
     1501 tagaacaggc tgaacagcgc cgcgcccgac agcagccggc cgcgccagat cagctcggca
     1561 tcgatctcgc gcccgcgcgg ggccagcgac agcggcacca gcgcgaaggt cggctgcagg
     1621 gtgatgtagg aatagtcgac cggcaggttg agcgcgagcc ccccgctttc gacccgcagc
     1681 ggctgggcca gccggaaggt cagcgaatcg ccgcgctgga acagcccctc gcggcccaga
     1741 tcgaaggccc aggccgaagt ggtcagccgc gatcccggag cgatcgaccc gccggtgtgc
     1801 ggccaggtaa agccgctgcg ccaggccgcg cccagccgcc agcgcggagc cgggcgccat
     1861 tcgccagagg catccaggaa caggctttcc gcgccgctgg cgccgaaccc ttcgtggaac
     1921 cgcgcgccca gcagggtccg gctttcgccc agccagcccg ccccgaacgt ggcggaaact
     1981 gggccaaagc ggcggtcgaa cgaaaccgaa agccggtcgg ccggatcgag ccgggtccgc
     2041 gcgacattgg tggcggcgct gatcaccggc gcggcggtca tcgccgagcc gtgctcggcc
     2101 ccgaaagtca cgccccagcc gccggcctgc cgccgccagg ccaggctgaa gctttcgtcg
     2161 cggccaaagc cgacatcgtc ggtcggcccg cgggcgatca ggaaggccgg ctggctgcgg
     2221 ccctgcagct gcgcgaccag cccgtccgac ccttgcgcaa aggcgaaggc gatgtcggaa
     2281 ttgggagcaa tccgggccac ggcccgggcc gccagaaccc gggcgatctc cgcgtcgcgg
     2341 tgcgacaggc gcagctgccc gctccacggc atccgggcga ccttgccgtt gccgtcgacc
     2401 gtgaaggcca tggtcaggtc ctccccgccg atcgtcacgc tgcggctttc gcgcgagagc
     2461 gccggaccga gccgcggctg gatctgggcg ctggtgaacc cggcggtcag gtcgatctgg
     2521 taagcccgct ggtaaccgtc gagcacgatc accccgatcc ccttggcggg ccggccggca
     2581 tcgcccatcg gcgccgaggt tacaccggtg ctgtcgccca gcggcatggc caccggactg
     2641 cccgccaggg tggtggtgcc ctgcggggca aaggccgccg caatatcgag gatcccgcgg
     2701 ccatagagcg gatcggtgcc cgcgtccccg gcatcgcgcg cggtgcgcag cagcaggtcg
     2761 accacctggg tcgcggtcag gttggggaag gcctggcgca gcagggccgc ggccccggcg
     2821 atctgcggcg cggcaaagct ggtgccggaa aagacataga ccgaacggct gccatcgtcg
     2881 ttggtcacga ccttcagcac gccgttttca tagacgcagc agacccgctc gccccgggcg
     2941 gaaaggtacc actgcgcttc ggtgccggcc ttgttcgaaa aggccgagat c
//
LOCUS       DASUDV010000117         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_843064,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000117 DASUDV010000000
VERSION     DASUDV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843064"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1416)
                     /locus_tag="VFV30_02560"
     CDS             complement(106..1416)
                     /locus_tag="VFV30_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX4847001.1"
                     /translation="MWPYHPDLLATPVPRYTSFPTAAEFAEGVGAVDADLALRQVEGT
                     ASLYLHIPFCEKICWYCGCNTGAANKAQRLASYLDALHRDIAITGTRLNRARIGRVAF
                     GGGSPNAIAPTDFVRLVDALTLHMPLADPDWSIELDPRTLTAEWGQVMGWVGVRYASM
                     GVQTFDPALQAAIGRVQPDADIARSVDLLRASGVSSLNFDLMYGLPGQGLAELETTLQ
                     RTIELGADRIALFGYAHVPHLLPRQRRIDAGALPGQDLRFAMAEFGFGLLTAAGYVPV
                     GFDHFARASDPMARAVAEGRLRRNFQGFTDDQADSLIGLGASAISCFPDLLVQNEKNA
                     GRYRMLLSQDRLPATLGVRRSADDRRRGAAIESLLCRSEAVLDADLIRAIADRLTPFA
                     QRGLARLDGNCLRIAPTGLPYARTIAALLDPYRQDSLRRFSSAV"
     gene            complement(1449..1694)
                     /locus_tag="VFV30_02565"
     CDS             complement(1449..1694)
                     /locus_tag="VFV30_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847002.1"
                     /translation="MIRVFARFGLLAMVPAALNVSAAAGATIRMPICTGDGQVRMVEV
                     PAGTPTGPGDEPAQCCVKGCHAGSSRKKLAKQIDPSQ"
     gene            complement(1691..1843)
                     /gene="ccoS"
                     /locus_tag="VFV30_02570"
     CDS             complement(1691..1843)
                     /gene="ccoS"
                     /locus_tag="VFV30_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX4847003.1"
                     /translation="MNGLAILIPVALGMGLFGLVAFFWALKRGQFEDLEGAAARILID
                     DEDGPA"
     gene            complement(1840..>2092)
                     /locus_tag="VFV30_02575"
     CDS             complement(1840..>2092)
                     /locus_tag="VFV30_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen fixation protein FixI"
                     /protein_id="HEX4847004.1"
                     /translation="VGRQAADLVFLGDSLLALPRAVRAARRTMAVVKQNFVMAIGYNL
                     LAVPAAMAGYVTPLFAAIAMSTSSLLVIGNSLRLARAVR"
BASE COUNT          404 a          714 c          676 g          298 t
ORIGIN      
        1 tggggcagaa agtcggcagc gaacgggcgg agactcacgg tttcctcccg gtcgatttct
       61 ccccacctgg agcggccggc gccaatgcct gccgctctcc ttttttcaga cagctgaact
      121 gaaccggcgc agcgaatcct ggcgataagg atccaatagc gcggcgatag tgcgcgcata
      181 gggcaggccg gtgggggcaa tgcgcaggca attgccgtca agccgggcca gcccgcgctg
      241 cgcaaacggc gtcagccgat cggcaattgc ccggatcagg tcggcatcaa gcacggcttc
      301 gctgcggcac agcaggcttt cgatcgccgc accgcggcgg cggtcgtccg cactgcgccg
      361 cacgccaagc gttgccggca gacgatcctg cgaaagcaac atgcgatagc gccccgcatt
      421 cttctcgttc tggaccagca gatcgggaaa acagctgatc gccgaggcgc caaggccgat
      481 caggctgtcg gcctgatcat cggtgaagcc ctggaaattg cgccgcagcc gcccttccgc
      541 cacggcccgg gccatcggat cgctggcccg cgcaaaatga tcgaagccga ccggaacata
      601 gcccgcagcc gtcagcaggc caaagccgaa ctcggccatg gcaaagcgca ggtcctgccc
      661 gggcagcgcg ccggcatcga tccggcgctg gcgcggcaag agatgcggca cgtgggcata
      721 gccgaacagg gcgatccgat cggcacccag ttcgatggtg cgctgcaagg tagtctcaag
      781 ctcggccagg ccctggcccg gaaggccata catcagatcg aaattgagcg agctgacccc
      841 gctggcgcgc agcagatcga ccgatctggc gatatcggca tcaggctgga cccgcccgat
      901 cgccgcctgc agcgcggggt cgaaggtctg cacccccatc gaggcatagc gcaccccgac
      961 ccagcccatc acctggcccc attccgcggt aagcgtgcgc ggatcgagct cgatcgacca
     1021 gtcgggatcg gccagcggca tgtgcagcgt cagtgcatcg accagccgga cgaaatcggt
     1081 gggcgcgatc gcattgggac tgccaccgcc aaaggccacg cggccgatcc tcgcccggtt
     1141 gaggcgtgtg ccggtgatcg cgatatcgcg gtgcagggca tcgagatagc ttgccagccg
     1201 ctgcgccttg ttggccgcac cggtattgca gccgcaatac cagcagatct tctcgcagaa
     1261 cgggatgtgg agatagagcg aagctgtgcc ttcgacctgg cgcagcgcaa gatcggcatc
     1321 caccgccccc accccttcgg caaactccgc agcggtcggg aagctggtgt aacgcggcac
     1381 cggggttgcc agcagatcgg gatgataggg ccacatgccc tgcgaattag gactggtggg
     1441 ggccgtggtc attgcgacgg gtcaatctgc ttggccagct tcttgcgcga ggagccagcg
     1501 tggcagccct tgacacagca ctgggccggt tcatcgcccg gtcccgtggg cgttccggcc
     1561 ggaacctcaa ccatccgcac ctggccatcg ccggtgcaga tcggcatgcg gatcgtggcc
     1621 ccggccgccg cagaaacatt gagcgcggcg ggaaccatcg ccagcaggcc aaagcgcgca
     1681 aagaccctga tcacgccggt ccatcctcgt catcgatcag gatccgcgcg gcggcacctt
     1741 cgagatcctc gaactggcca cgcttcagcg cccagaagaa ggccaccagc ccgaacaggc
     1801 ccatgcccag cgccaccggg atcaggatcg ccagcccgtt catcgcacag ccctggcaag
     1861 gcgcagcgaa ttgccgatca ccagcagcga ggaggtcgac atcgcgatcg cggcgaacaa
     1921 cggggtcaca tagccggcca tggctgcggg aaccgccagc aggttgtagc cgatcgccat
     1981 cacgaagttc tgtttcacca cggccatggt ccgccgcgca gcgcgcaccg cgcggggcag
     2041 cgccagcagc gaatcgccca ggaacaccag atcggcggcc tggcggccca cg
//
LOCUS       DASUDV010000118         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1793019,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000118 DASUDV010000000
VERSION     DASUDV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793019"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="leuB"
                     /locus_tag="VFV30_02580"
     CDS             complement(<1..737)
                     /gene="leuB"
                     /locus_tag="VFV30_02580"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX4847005.1"
                     /translation="MKIAVLAGDGIGPEVTREAVRVLEALALPGLELSYADVGGAAYK
                     AHGHPLPPETLAIARASDAILFGAVGDPDCDNLERHLRPEQAILGLRKELGLFANLRP
                     AKVFKGLEDASALRPEVAGAIDLLIVRELNGDVYFGEKGIRTLPDGRRQGWDMMAYAE
                     DEVRRIAHVGFRAARGRQGRLCSVDKANVLETSQVWRDVMIEVAAEYPDVALSHMYVD
                     NAAMQLVRSPGQFDVIVTGNLFGDILS"
     gene            complement(745..1332)
                     /locus_tag="VFV30_02585"
     CDS             complement(745..1332)
                     /locus_tag="VFV30_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847006.1"
                     /translation="MQIRSPAIVCASRPHGETAAIVRALTPEDGMVAGYVAGGRGRQL
                     RPVLIPGNAVEMDLRARSEGQLPFARLELTQSRAPWLAEPLPAAAIGWVTALTAATLP
                     ERQAYPSLYTALSGLLDAVCHAPSARGWLPGMIAYEALLMRELGYGGQAPEADAALAA
                     FDAQGARLERYLLADRRGDVMGARAMLRERLARMG"
     gene            complement(1335..1595)
                     /locus_tag="VFV30_02590"
     CDS             complement(1335..1595)
                     /locus_tag="VFV30_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX4847007.1"
                     /translation="MQSENPLIADIAKLLNSAAGTVAGMGREARDAARERVKEQLGGL
                     DFVTREEFDTVKLMAAKAREENEALKARLEVLEAALNAKAKK"
     gene            complement(1627..>2227)
                     /locus_tag="VFV30_02595"
     CDS             complement(1627..>2227)
                     /locus_tag="VFV30_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX4847008.1"
                     /translation="GPGRGHYPPEIGSDSRFLSMSELASPGQLRAALLRWSLFLAPLC
                     LLIGSFVGRVAGSGANNAWFMGLEKPAIYPPPVTFGIVWTILYLMMGVALAMIVTARG
                     APGRGVAIGVFVAQFLLNLAWSPVFFGMHQLQNALYLLIAIDVAVLVTVYLFWKVRPL
                     AAWLLLPYLAWVLFATVLNWQFVAANPEAAAGIEVAMAS"
BASE COUNT          363 a          796 c          742 g          326 t
ORIGIN      
        1 tcggacagga tatcgccaaa caggttgccg gtgacgatca cgtcgaactg gccggggctg
       61 cgcaccagct gcatcgcggc gttgtcgaca tacatgtggc tgagcgcgac atcggggtat
      121 tcggccgcaa cctcgatcat cacgtcgcgc cagacctggc tggtttccag cacgttggcc
      181 ttgtcgaccg agcagagccg gccctgccgg ccgcgggccg cgcggaaacc gacgtgagcg
      241 atgcgccgca cctcgtcctc ggcataggcc atcatgtccc agccctggcg gcggccgtcc
      301 ggcagggtgc ggatgccctt ttcgccgaaa tagacatcgc cgttgagctc gcggacgatc
      361 agcagatcga tcgcgccggc cacttcgggg cgcagcgccg aggcgtcttc cagccccttg
      421 aacaccttgg ccgggcgcag gttggcaaac agccccagct ccttgcgcag ccccaggatc
      481 gcctgctcgg gccgcaggtg gcgttccagg ttgtcgcaat ccggatcgcc gaccgcgccg
      541 aacaggatcg cgtcggacgc gcgggcgatc gccagcgtct cgggcggcag cgggtggccg
      601 tgggccttgt aggccgcgcc gccgacatcg gcatagctca gttccagccc gggcagcgcc
      661 agcgcctcca gcacgcgcac cgcttcgcgc gtcacttcgg ggccaatgcc gtcgccggcc
      721 aggactgcga tcttcatgtc tgtgttaccc catccgtgcc agccgctcgc gcagcatggc
      781 gcgcgcgccc ataacatcgc cgcgccgatc tgcaagcagg tagcgttcca gccgcgcgcc
      841 ctgcgcgtcg aaggcggcca gcgcggcatc ggcttcgggc gcttgcccgc catagcccag
      901 ctcgcgcatc agcagcgctt cataggcgat catcccgggc agccagcccc gcgccgaagg
      961 ggcgtggcag accgcatcga gcaggcccga aagcgcggtg tagagagacg gataggcctg
     1021 ccgctcgggc agggtcgcgg cggtcagcgc ggtgacccag ccgatcgccg cggccggcag
     1081 cggctcggcc agccaggggg cgcggctttg ggttagctcc agccgggcaa agggtagctg
     1141 cccctcggac cgggcgcgca ggtccatctc caccgcgttg ccggggatca gcaccgggcg
     1201 cagctgccgc ccgcggccac cggcgacata gccggcgacc atgccgtcct ccggggtcag
     1261 cgcccgcacg atcgccgccg tctcgccgtg ggggcgtgac gcgcagacaa tggcgggact
     1321 gcggatttgc agggctactt cttcgccttg gcgttcagcg cggcttccag cacttcgagc
     1381 cgggccttca gcgcctcgtt ctcttcgcgg gccttggcgg ccatcagctt gaccgtgtcg
     1441 aactcctcgc gggtgacgaa gtcgagcccg ccaagctgtt ccttgacccg ctcccgcgcg
     1501 gcatcgcgcg cttcgcggcc catgccggca acggttccag ccgcgctgtt cagcagcttg
     1561 gcaatatcgg cgatcagggg gttttcgctc tgcatggcgg ctagatggtc ctttgcggcg
     1621 ctgcgatcaa gaggccatgg ccacttcgat cccggcggcc gcttcgggat tggccgccac
     1681 gaactgccag ttgagcaccg tggcgaacag cacccaggcc agatagggca gcagcagcca
     1741 ggccgccagc gggcgcacct tccagaacag gtagaccgtg accagcaccg ccacgtcgat
     1801 cgcgatcagc aggtagagcg cgttctgcag ctggtgcatc ccgaaaaaca ccggcgacca
     1861 ggccaggttc agcaggaact gcgccacgaa cacgccgatc gccacgcccc gccccggcgc
     1921 gccgcgcgcg gtcacgatca tggcgagcgc cacgcccatc atcaggtaaa ggatggtcca
     1981 gacgatcccg aaagtgaccg gcggcggata gatcgcgggc ttttccagcc ccatgaacca
     2041 ggcgttgttg gccccgctgc ccgccacccg gccgacaaag ctgccaatca gcaggcacag
     2101 cggcgccagg aacagcgacc agcgcagcag ggcggccctg agttggccgg gcgaagcaag
     2161 ttcactcatc gaaaggaacc tcgaatcaga gccgatttcc gggggatagt gaccgcgccc
     2221 cggcccc
//
LOCUS       DASUDV010000119         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1063999,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000119 DASUDV010000000
VERSION     DASUDV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063999"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFV30_02600"
     CDS             <1..585
                     /locus_tag="VFV30_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX4847009.1"
                     /translation="AAAAGGKQEVMVAGVAGLVAGALSMAAGEYVSVSSQADTEAADL
                     ARERKELAEQPEFELAELTQVYRQRGLDEALARQVAEALTAHDALTTHARDELGLAEM
                     HRARPLQAALASAATFAVGAVLPLVTAMLLTLGTIPVGVSIASLFFLMLLGAVGAKAG
                     GAPILKGTLRVTFWGVIAMAATYAIGSLFGTSVA"
     gene            complement(1058..1450)
                     /gene="clpS"
                     /locus_tag="VFV30_02605"
     CDS             complement(1058..1450)
                     /gene="clpS"
                     /locus_tag="VFV30_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX4847010.1"
                     /translation="MRYFTPMIDHTALNLTPLCNDGDADRDGDAGGQGQVGLATKTRA
                     KPKKPSQFKVLMLNDDYTPMEFVVMVLKRFFSMDLEQATRVMLHVHQRGVGVCGIFPY
                     EIAETKVNQVMDFARQNQHPLQCTLEKA"
     gene            complement(1584..2357)
                     /locus_tag="VFV30_02610"
     CDS             complement(1584..2357)
                     /locus_tag="VFV30_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX4847011.1"
                     /translation="MAEATEAKIDAAKAEAAAEKAYAAAAAAAAVDAKVEAKPETKAK
                     PAPAAAKVEAKTPAAKPAATKPVAVKKAAAKPATAKKVPAKKTAKAPAKKAAVAKKPT
                     PSFKDTIMTKTQTVTEDFTAKVKDAVSDAQDRAKLAFEKSQAMFGEAGEFTKGNVEAL
                     VESGKILAAGVQDLGKDYVAEAKTAFETVQGDIKDLTAVKSPADFFKLQGEILRRNFD
                     AAVAAGSKNSEKVVKIAGEAFAPIQSRVSLAIEKVKQAA"
     gene            complement(2502..4271)
                     /gene="phaC"
                     /locus_tag="VFV30_02615"
     CDS             complement(2502..4271)
                     /gene="phaC"
                     /locus_tag="VFV30_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX4847012.1"
                     /translation="MPAADLAQWTQVMQRLQALWLEFQNEQAGGALAKLPEMLSDPGG
                     FAGIMQGWLKALPLANPATQQRMWEDSQALWATVLGQYGIGRKAGEAGADGPELPRKD
                     RRFADPKWREQPFYALVHQTYLMLAEQVLAMADAAASGDPARGEQLRFAARTLVEMLS
                     PAHFPMLNPLVMEKAMATKGESLVKGFEHLLADLKRGQLTHVDPEAFKLGVNLAATPG
                     KVVHQTPLYQLVQYSPATESVLQVPLVIFPPWINRFYILDLTPQKSFIRWAVEQGLTV
                     FVVSWKSADASMADLVWDDYIKAQIDAIDTVRARLDVPAVHTIGYCVAGTTLAATLAV
                     LARRGEADKVKSATFFTAQVDFEKAGELKVFIDDQQLAALEKLSAQTGYLDGRYMSAT
                     FNLLRGNDLIWNYVVKNYLLGEDYPAFDLLHWNGDVTNLPAKWHMSYLKDLYRDNKLV
                     VPDALSADGTPIDLTRIETPAYIQAGREDHIAPAESVWRLMHHLRGDKTFLLAGSGHI
                     AGVVNPPAARKYQYWTNPGPADSLAEFVAGAVETPGSWWPHWIEWIRRQDQGEVPAKG
                     KRVPGGKGDTVLEEAPGSYVKAP"
     gene            complement(4377..>4881)
                     /locus_tag="VFV30_02620"
     CDS             complement(4377..>4881)
                     /locus_tag="VFV30_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847013.1"
                     /translation="DRSPEELAMPALSHRFAAVSIGLAAALTAAAPLAAKAPDSPYWG
                     RWTVNDKKAVFSGRGREYRTIDIAPCGKDFCGVSVGENGRCGPVLFRFLMKRAPNGDE
                     LRGHGKWGNGRKNVMIWLFDYEDGNLRRNMQLYLGDGYDFGERSENMPKFEAVYRKSG
                     VARCTAR"
BASE COUNT          765 a         1542 c         1637 g          937 t
ORIGIN      
        1 gctgccgcgg ccggcggcaa gcaggaggtg atggtggccg gtgtggccgg gctggtcgcc
       61 ggcgcgcttt ccatggcggc gggggaatat gtctcggtct cgagccaggc cgataccgag
      121 gcggcggacc tggcgcggga acgcaaggag ctggccgaac agcccgaatt cgagctggcc
      181 gaactgaccc aggtctaccg ccagcgcggg ctggacgagg cgctggcccg gcaggtcgcc
      241 gaagccctga ccgctcacga cgccctgacc acccatgccc gcgacgaact gggcctggcc
      301 gaaatgcacc gcgcccgtcc gctgcaggcg gcgctggcct cggccgcgac ctttgcggtg
      361 ggggcggtgc tgccgctggt gacggcgatg ctgctcacgc tcggcacgat cccggttggc
      421 gtctcgatcg cctcgctgtt cttcctgatg ctgctgggcg cggtcggcgc caaggccggc
      481 ggcgcgccga tcctgaaggg cacgctgcgg gtgaccttct ggggcgtgat cgcgatggcc
      541 gcgacctatg ccatcggcag cctgttcgga acgagcgtgg cctagatcag aagtcggatt
      601 tgcgattgta cgaacggccg caaatctgcg ggtaacgcgt gcctgattgg ctggaaattg
      661 ggggggcggg ccttaagcct tggggccatt ctctttggtg gcggcgcgac gcttccaaag
      721 ggcttgagtg atgtccagga ggtttacgag cacaaggagt ccaaagattg gcaggatcca
      781 atcgtcaggc aaactgggca agaaaccaaa cgaaggggcc gcccaacaat acgcgaagaa
      841 agcgagacct cccagccaaa gatagaccga gttacgcgca tctgcactca caggaggagc
      901 tcctcgtgtt tagcttaatc gacaaattca ggataaggtt tgcagataga cgaaccttcc
      961 gcaagattgt cgtttccgaa acggtaccct ttagcccgaa ctcttcaatc ctgtcaccgg
     1021 gcgcaaccat gcccgctggc ctcggttcag cccggaatca cgccttttcc agcgtgcact
     1081 gcagcgggtg ctggttctgg cgggcgaagt ccatcacctg gttcaccttg gtttcggcga
     1141 tttcataggg aaagatcccg cagacgccga ctccgcgctg gtggacgtgc agcatcaccc
     1201 gggtggcctg ttccaggtcc atgctgaaga accgcttcag caccatcacc acgaattcca
     1261 tcggggtgta atcgtcgttc agcatcagca ctttgaactg gctgggcttt ttcggcttgg
     1321 cgcgggtctt ggtcgcgagg ccgacctggc cctggccgcc ggcatcgccg tcacggtcgg
     1381 catcgccgtc gttgcacagc ggcgtcaggt tcaaggcggt gtgatcgatc atcggggtga
     1441 aatatcgcat cgaaggggtt aaggacaagt gggcacggcc atagccgaaa agggccggac
     1501 agcgcagtgc tgtccggccc tttggatttg gctcgcggcc cggcggggag aggggagcgc
     1561 cggcccgcga ggaacggctg cccttaggcg gcctgcttga ccttttcgat cgccaggctg
     1621 acgcggctct ggatcggggc gaaggcttcg ccggcgatct tgacgacctt ttccgagttc
     1681 ttcgaaccgg cggcaaccgc ggcgtcgaag ttgcggcgca ggatttcacc ctgcagcttg
     1741 aagaagtcgg ccgggctctt caccgcggtc aggtccttga tgtcgccctg aacggtttcg
     1801 aaagcggtct tggcttcggc gacatagtcc ttgccgaggt cctgcacgcc agcggcgagg
     1861 atcttgcccg attcgaccag ggcttcaacg ttgcccttgg tgaattcgcc ggcttcgccg
     1921 aacatcgcct ggctcttttc gaaggccagc ttggcgcggt cctgggcgtc ggaaacggcg
     1981 tccttgacct tggcggtgaa gtcttcggtg acggtctggg tcttggtcat gatggtgtcc
     2041 ttgaaagagg gggtgggctt cttggccacg gccgccttct tggcgggggc cttcgcggtc
     2101 ttcttggccg gcaccttctt ggcggtggcg ggcttggcag cagccttctt cacggcgacc
     2161 ggcttggtcg cggcgggctt ggcagccggg gtcttcgctt cgaccttggc agcggccgga
     2221 gccggcttgg ccttggtttc gggcttcgct tcaaccttgg cgtccaccgc ggcggcagca
     2281 gcggcagcgg cataggcctt ttcggccgcg gcttcggcct tggcggcgtc gatcttggct
     2341 tcggtggctt cggccattgt ctcaaatcct tcgcaagggg tctgcaccgg acatccggca
     2401 cggctcatgt tgcagtgcac aaaataggta ttgcacttgc gaagtcaagg attttttgtg
     2461 cagtgcagca taaaccgcaa ctgcgctgga aaatcagggg tttagggcgc tttgacgtag
     2521 ctgcccgggg cctcttccag caccgtatcg ccctttccgc caggcactcg cttgcccttg
     2581 gcgggcactt cgccctggtc ctggcggcgg atccattcga tccagtgcgg ccaccagctg
     2641 ccgggcgtct cgaccgcgcc ggccacgaat tcggccaggc tgtcggccgg gccagggttg
     2701 gtccagtact ggtatttcct ggctgcgggc ggattgacca ccccggcaat gtggccggac
     2761 cccgccagca ggaaggtctt gtctccgcgc aggtggtgca tcagccgcca gacgctttcg
     2821 gccggggcaa tgtgatcctc gcggccggcc tggatatagg ccggggtctc gatccgggtc
     2881 aggtcgatcg gggtgccatc ggcggacagg gcatcgggca cgaccagctt gttgtcgcgg
     2941 tagaggtcct tcaggtagct catgtgccac ttggccggca ggttggtgac atcgccgttc
     3001 cagtgcagca ggtcgaaagc cggatagtcc tcgcccagga ggtagttctt gacgacgtag
     3061 ttccagatca ggtcgttgcc gcgcagcagg ttgaaggtgg ccgacatgta gcgcccgtcg
     3121 agatagccgg tctgggccga aagcttttcc agcgcggcca gctgctggtc gtcgatgaac
     3181 accttcagct cgcccgcctt ctcgaaatcg acctgggcgg tgaagaaggt cgcgctcttg
     3241 accttgtccg cctcgccccg ccgggccagc acggccagcg tcgcggcgag cgtggtgcct
     3301 gccacgcaat agccgatggt gtggaccgcc ggcacgtcca gccgcgcgcg gaccgtgtcg
     3361 atcgcatcga tctgggcctt gatgtaatcg tcccagacca ggtcggccat gctcgcgtcg
     3421 gccgatttcc aggacaccac gaagacggtc agcccctgct ccaccgccca gcggatgaag
     3481 cttttctgcg gcgtcaggtc gaggatgtag aaccggttga tccatggcgg gaagatcacc
     3541 agcggcactt gcagcacgct ttccgtggcc gggctgtact ggaccagctg gtacagcggg
     3601 gtctggtgga ccaccttgcc gggggtggcg gccaggttca cgcccagctt gaaggcttcg
     3661 gggtcgacgt gggtcagctg cccgcgcttg agatcggcca gcagatgctc gaaccccttg
     3721 accaggcttt cgcccttggt cgccatggcc ttttccatga ccagcgggtt gagcatcggg
     3781 aaatgcgccg ggctgagcat ttcgaccagg gtccgcgcgg caaagcggag ctgctcgccg
     3841 cgggccggat cgccgctggc ggcggcatcg gccatggcca gcacctgttc ggccagcatc
     3901 aggtaggtct ggtggaccag cgcgtagaac ggctgctcgc gccatttcgg atcggcaaag
     3961 cggcggtcct tgcgtggcag ctccggcccg tcagcgccgg cttcacccgc cttgcgcccg
     4021 atcccgtact ggcccagcac cgtggcccac agcgcctggc tgtcttccca catccgttgc
     4081 tgggttgccg gattggccag cggcagcgcc ttcagccagc cctgcatgat cccggcaaaa
     4141 ccgcccggat cggacagcat ctcgggcagt ttggccagcg ccccgccggc ctgctcgttc
     4201 tggaattcca gccacagcgc ctgcagccgc tgcatcacct gggtccactg ggccaggtcc
     4261 gcggccggca tttcgccgcc ggcgggcagg aacgggctga gcagctgctg cggcgcggta
     4321 aacagcgcgc cgagcagttc ggtcgggtcc ttcgggtcgg tcattgcccc cttgtatcag
     4381 cgggcggtgc agcgcgcgac cccgctcttg cggtaaacgg cctcgaattt cggcatgttc
     4441 tcggaccgct cgccgaaatc gtagccgtcg ccgaggtaga gctgcatgtt gcggcgcagg
     4501 ttgccatcct cgtaatcgaa cagccagatc atcacgttct tgcgcccatt gccccatttg
     4561 ccgtggccgc gcagttcatc cccgttcggc gcgcgcttca tcaggaagcg gaacagcacc
     4621 gggccgcacc ggccgttttc gccgacgctc accccgcaga agtccttgcc gcagggggcg
     4681 atgtcgatcg tccggtattc gcggccgcgg cccgaaaaga ccgccttctt gtcgttcact
     4741 gtccagcgcc cccaataggg gctgtcggga gccttggcgg ccagtggcgc agccgccgtc
     4801 agcgcggcgg caaggccaat cgacacggcg gcaaagcggt gggataaggc aggcatggca
     4861 agctcctctg gcgatcgatc c
//
LOCUS       DASUDV010000120         5195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1947528,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000120 DASUDV010000000
VERSION     DASUDV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947528"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /gene="infB"
                     /locus_tag="VFV30_02625"
     CDS             <1..1617
                     /gene="infB"
                     /locus_tag="VFV30_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX4847014.1"
                     /translation="TAELLVEEFGHNIQRVSESDVDIVASDDVDAEDTLKPRPPVVTI
                     MGHVDHGKTSLLDALRGTDVVRGEAGGITQHIGAYQIKTKGGDLITFLDTPGHEAFTE
                     MRQRGANVTDIVILVVAGDDGLMPQTIEAINHTKAAGVPMIVAITKSDKPEFNPQKIR
                     ERLLEHEVIVEAMSGDVQDVEVSAKTGAGLDNLIEKILLQSELMELKANPDRPAEATV
                     IEAKLDKGKGPLATVLINRGTLKVGDILVVGTHSGRVRAMLDDKGRQIKVAPPSLPVE
                     VLGIGGVPMAGDTLTVVESETRAREVAAYRQEKATAKRTTTAPASLENMFSALKAKAD
                     VIEYPVVVKSDVQGSTEAIVNALNKLSNDEIKVKVLQSGVGAITESDVSLAAATGAPI
                     IGFHVRPNAKAREMIERNKVRMKYFDVIYHLTDEVAKEMAGIWGPERIEHVVGRAEVK
                     EVFPAGKRDKAAGLLVLEGGIRKGLFARLTRQDVIVSKTTIASLRRFKDDVPEVRAGL
                     ECGVVLQDTNDIKPGDMLEVFEVEERERTL"
     gene            1614..2024
                     /locus_tag="VFV30_02630"
     CDS             1614..2024
                     /locus_tag="VFV30_02630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4847015.1"
                     /translation="MSDLTETRGAHAHLMGAQYLSFDRESETITMRFTAPDSFVSPRG
                     SVQGGLVAGFLDEAMGWAHVMATEGAEAPLNLDINMTLLKPVLPGPLLAKGRVVRRGK
                     RVIFLEGELLDEGGAVLARSTSTAIPTPRPGDQG"
     gene            2044..2580
                     /locus_tag="VFV30_02635"
     CDS             2044..2580
                     /locus_tag="VFV30_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX4847016.1"
                     /translation="MPRYVALFGSINVGGNRLTMADLRWAFEREGFENVETVVASGNV
                     LFEFDERPTDGLEELFAHMMADRFDIDSFVAVRSAEEIAAALAANPFAADGAPNLVHV
                     HFLGGQPDEAAFARLLADHAGRGAERLAAGDRALHIDYVDGAGHSKLTGAFIQRRLGF
                     PGTARNIRSLQRILDKMT"
     gene            2580..2969
                     /gene="rbfA"
                     /locus_tag="VFV30_02640"
     CDS             2580..2969
                     /gene="rbfA"
                     /locus_tag="VFV30_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX4847017.1"
                     /translation="MARPAATPETRSVRLLKVGEQVRHVISELLTRQMVHDEVLTAHS
                     VSVTEVRMSPDLRHATVFVKPLLGSDEELVLKALRTNTAFFQKEVAGKLRTKYAARIK
                     FLPDESFDEASRIDALLADPKVQRDLD"
     gene            3041..3571
                     /locus_tag="VFV30_02645"
     CDS             3041..3571
                     /locus_tag="VFV30_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEX4847018.1"
                     /translation="MTTYTAFAEGLWLATGSRAHIVHALKAMPQMPRAGDILVFDDAN
                     GRQVDFDLRGEPEPEPAAGPGRPRLGVVAREVTLLPRHWDWLKDQPGGASAALRRLVD
                     GASRAPLSAAAGRDAAYRFLSAIAGDRENYEEAIRALYQDDRARFLELTAGWPPAVRD
                     HAAAFAWPVSPEPSAP"
     gene            complement(3546..4106)
                     /locus_tag="VFV30_02650"
     CDS             complement(3546..4106)
                     /locus_tag="VFV30_02650"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX4847019.1"
                     /translation="MRIVLALLLLFATASAGPAYRYRLDGAGSQISARVSYFGVGHKT
                     ARFPAMRGSIRLSPDRLDAIDLEVELDARAMTAGSKTDTAYLKGKDFFDVANHPAVRF
                     SGNRMAMTGPQTARVDGQVTVRGITRPVTLVVTFADPPARASGREPIALTATTTINRR
                     DFGMTAYNVVVGKKVTITIKARMVPD"
     gene            4202..4780
                     /locus_tag="VFV30_02655"
     CDS             4202..4780
                     /locus_tag="VFV30_02655"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX4847020.1"
                     /translation="MAKLYFYYASMNAGKSTTLLQADFNYRERGMATMLWTAALDTRS
                     DHQAIESRIGLGADAHRFAPDTDLWERVTAAHRVEPLACVLIDEAQFLTEGQVWQLAR
                     LADQANIPVLCYGLRTDFQGELFPGAKVLLGIADSLVELKAVCHCGRKSTMNLRVDAS
                     GAAVKQGAQTEIGGNDRYVALCRKHFVEALEG"
     gene            4784..>5195
                     /locus_tag="VFV30_02660"
     CDS             4784..>5195
                     /locus_tag="VFV30_02660"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEX4847021.1"
                     /translation="MRSPMIAAILALACGAAAQAKPLPAPPAWPELGVETRIPFPDRT
                     IRDFEADTDEGIWLQDNRRRWYYGRFIGNCPGLQFAQAIAFDTRGSAEFDKHSVILIE
                     REKCFLSSLVTSERPVPRNQRNKPAPAGGNETPKP"
BASE COUNT          917 a         1752 c         1732 g          794 t
ORIGIN      
        1 acggccgagc tgctggtcga ggaattcggc cacaacatcc agcgcgtttc ggaaagcgat
       61 gtcgacatcg tcgcttcgga cgacgtcgat gccgaggaca cgctgaagcc gcgtccgccg
      121 gtggtgacga tcatgggcca cgtcgatcac ggcaagacca gcctgctcga cgcgctgcgc
      181 ggcaccgatg tggtgcgcgg cgaagccggc gggatcaccc agcacatcgg cgcctaccag
      241 atcaagacca agggcggcga tctgatcacc ttcctcgaca ccccgggcca cgaagccttt
      301 accgaaatgc gccagcgcgg cgccaacgtg accgacatcg tgatcctggt cgtggccggt
      361 gacgacgggc tgatgccgca gacgatcgag gcgatcaacc acaccaaggc ggccggcgtg
      421 ccgatgatcg tggcgatcac caagagcgac aagccggaat tcaacccgca gaagatccgc
      481 gagcgcctgc tggagcacga agtgatcgtc gaagcgatga gcggcgacgt ccaggatgtc
      541 gaagtctcgg ccaagaccgg tgcgggcctc gacaacctga tcgagaagat cctgctccag
      601 tcggaactga tggagctgaa ggccaaccct gaccgccccg ccgaagcgac cgtgatcgaa
      661 gccaagctgg acaagggcaa gggcccgctg gcgaccgtgc tgatcaaccg cggcacgctg
      721 aaggtcggcg atatcctggt ggtcggcacc cattccggcc gcgtccgcgc gatgctggat
      781 gacaagggcc gccagatcaa ggtggcgccg ccgtcgctgc cggtcgaagt gctggggatc
      841 ggcggcgtgc cgatggccgg ggacaccctg accgtggtcg aaagcgaaac ccgcgcccgc
      901 gaggttgccg cctaccgcca ggagaaggcc accgccaagc gcaccaccac ggctcccgcc
      961 agcctggaaa acatgttctc ggcgctgaag gccaaggccg acgtgatcga atacccggtc
     1021 gtggtgaaga gcgacgtcca gggttcgacc gaagcgatcg tcaacgcgct gaacaagctt
     1081 tcgaacgacg agatcaaggt caaggtgctg caatcggggg tcggcgcgat caccgaaagc
     1141 gacgtgagcc tggcggccgc caccggcgcg ccgatcatcg gcttccacgt ccgtcccaac
     1201 gccaaggcgc gcgagatgat cgagcgcaac aaggtgcgga tgaagtactt cgacgtgatc
     1261 tatcacctga ccgacgaagt ggccaaggaa atggccggga tctggggtcc ggagcggatc
     1321 gagcacgtgg tcggccgcgc cgaggtcaag gaagtgttcc cggccggcaa gcgcgacaag
     1381 gccgccggcc tgctggtgct ggaaggcggg atccgcaagg gcctgttcgc gcgccttacc
     1441 cgtcaggacg tcatcgtttc gaagaccacc atcgccagcc tgcgccgctt caaggacgat
     1501 gtccccgaag tccgcgccgg tctggaatgc ggcgtggttc tgcaggacac caacgacatc
     1561 aagccgggcg acatgcttga agtcttcgag gtcgaggagc gtgaacgcac gctgtgagtg
     1621 acctgaccga aacccgcggc gcccatgccc atctgatggg cgcccagtac ctctcgttcg
     1681 accgcgagag cgagacgatc accatgcgct tcaccgcgcc ggacagcttc gtctcgccgc
     1741 gcggttcggt gcagggcggg ctggtcgcgg ggtttctgga cgaggcgatg ggctgggccc
     1801 atgtcatggc gaccgagggg gcggaggctc cgctcaacct cgatatcaac atgaccctgc
     1861 tgaagccggt cctgcccggg ccgctgctgg ccaagggccg ggtggtgcgg cgcggcaagc
     1921 gagtgatctt cctggaaggc gaactgctgg acgagggcgg cgccgtgctg gcccgctcca
     1981 cctccaccgc cattcccacc ccgcgccccg gcgatcaggg gtgagcggcc cggtccgcgc
     2041 ctgatgccgc gctatgtcgc cctgttcggc agcatcaatg tcggcggcaa ccggctgacc
     2101 atggccgacc tgcgctgggc gttcgagcgc gaggggttcg aaaacgtcga gacggtggtg
     2161 gccagcggca acgtgctgtt cgaattcgac gagcggccga ccgacgggct ggaagagctg
     2221 ttcgcccaca tgatggccga ccggttcgac atcgacagct tcgttgcggt gcgcagcgcc
     2281 gaggagattg ccgccgcgct cgccgccaat ccctttgccg ccgatggcgc gcccaacctg
     2341 gtccacgtcc acttcctggg cggccagccg gacgaagcgg cctttgcccg gctgctggcc
     2401 gatcacgccg ggcgcggcgc agagcggctt gcggccggcg acagggcgct ccatatcgat
     2461 tatgtcgacg gggccgggca ttccaagctg accggcgcct tcatccagcg gcgcctgggc
     2521 tttccgggca ccgcccgcaa catccgctcg ctgcagcgca tactggacaa gatgacctga
     2581 tggcccgccc cgccgcaacc cccgaaaccc gctcggtccg cctgctcaag gtgggcgaac
     2641 aggtccgcca tgtgatcagc gagctgctga cccggcagat ggtccatgac gaggtgctga
     2701 ccgcccattc cgtctcggtc accgaagtgc ggatgagccc ggacctgcgc cacgcgacag
     2761 tcttcgtgaa gccgctgctc ggctcggacg aggaactggt gctgaaggcg ctgcgcacca
     2821 acaccgcctt tttccagaag gaagtggccg gcaagctgcg caccaaatac gccgcgcgga
     2881 tcaagttcct gccggacgag agctttgacg aagccagccg gatcgacgcc ctgctggccg
     2941 atcccaaggt ccagcgcgac ctggactaag gtatttttac ccgggtgtat tgaccggatt
     3001 attacccggg tataaagcag gcttcgcaaa ggagcccgcc atgaccacct ataccgcttt
     3061 cgccgagggc ctctggctcg ccacgggcag ccgtgcgcac attgtccatg cgctgaaagc
     3121 catgccgcaa atgccgcggg ccggcgatat cctggtcttc gatgatgcca acggccgcca
     3181 ggtcgatttc gacctgcggg gcgagcccga gccggaaccg gccgcggggc cgggccggcc
     3241 gcggctcggc gtcgtcgccc gcgaagtcac cctgctgccg cgccattggg attggctgaa
     3301 ggatcagccc ggcggagcct cggccgcgct gcggcggctg gtggacgggg ccagccgcgc
     3361 gccgctttca gccgcagccg ggcgggacgc ggcctaccgc tttctctccg ccatcgccgg
     3421 ggatcgcgag aactacgagg aagcgatccg cgccctctat caggacgacc gcgcgcggtt
     3481 tctggagctg accgccggct ggccgcccgc cgtccgcgat cacgccgccg cctttgcctg
     3541 gccggtcagt ccggaaccat ccgcgccttg atcgtgatcg tcaccttctt gcccaccacc
     3601 acgttgtagg ccgtcatccc gaaatcacgg cggttgatcg tggtggttgc ggtcagggca
     3661 atcggttcac gcccgctggc ccgggccggg ggatcggcaa aggtgaccac cagggtcacc
     3721 ggccgggtta tcccgcgcac ggtcacctgg ccgtcgaccc gcgcggtctg cggcccggtc
     3781 atcgccatgc ggttgccgct gaaacgcacc gccgggtggt tggcgacgtc gaagaagtcc
     3841 ttgcccttga gataggcggt atcggttttg ctgccggcgg tcatcgcccg ggcgtcgagc
     3901 tccacctcca ggtcgatcgc gtcgagccgg tcgggcgaga ggcggatcga gccgcgcatc
     3961 gccggaaagc gcgcggtctt gtgaccgacc ccgaaatagg acacccgcgc gctgatctgc
     4021 gagcccgcgc catcgagccg atagcgatag gccggccccg ccgaggcggt ggcgaaaagc
     4081 agcagcaagg caagaacgat ccgcaccggg cgacccctgt atttgctcag ggattggcga
     4141 tccacagccg ccctgtcaat cgcgctggtc ttttggcgat cagtgcttat ctcatgccgg
     4201 catggccaag ctctatttct actacgccag catgaacgcg gggaaatcga ccacgctgct
     4261 gcaggccgat ttcaattacc gcgagcgcgg catggccacc atgctgtgga ccgcggcgct
     4321 cgatacccgc agcgatcacc aggcgatcga aagccggatc gggctgggcg ccgatgccca
     4381 ccgctttgcc ccggacaccg acctgtggga gcgggtgacc gccgcccacc gggtggagcc
     4441 gctggcctgc gtgctgatcg acgaggccca gttcctgacc gagggccagg tctggcagct
     4501 ggcccggctg gccgatcagg ccaacatccc ggtgctgtgc tatggcctgc ggaccgattt
     4561 ccagggtgag ctgtttcccg gggccaaggt gctgctgggc attgccgaca gcctggtgga
     4621 gctcaaggcg gtgtgccact gcggccgcaa atcgaccatg aacctgcggg tcgatgccag
     4681 cggcgcggcg gtgaagcagg gcgcccagac cgagattggc gggaatgacc gctatgtcgc
     4741 cctgtgccgc aagcatttcg tcgaagcact ggaaggatag accatgcgca gcccgatgat
     4801 tgccgcgatc ctggccctgg cctgtggcgc cgccgcccag gccaagccgc tgcccgcgcc
     4861 gccggcctgg ccggaactgg gggtggaaac ccgcattccc tttcccgacc gcacgatccg
     4921 cgatttcgaa gccgataccg atgaaggcat ctggctgcag gacaaccgcc ggcgctggta
     4981 ctacggccgc ttcatcggca actgcccggg gctgcaattc gcccaggcga tcgcctttga
     5041 cacccgcggt tcggccgagt tcgacaagca cagcgtgatc ctgatcgagc gcgagaagtg
     5101 cttcctgtcc agcctggtca cgtccgaacg gcccgtgccc cggaaccagc gcaacaagcc
     5161 ggcccccgcc ggcggaaacg agacccccaa accat
//
LOCUS       DASUDV010000121         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_511940,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000121 DASUDV010000000
VERSION     DASUDV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511940"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFV30_02665"
     CDS             complement(<1..327)
                     /locus_tag="VFV30_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847022.1"
                     /translation="MPNTRRALSVLLLGLTGWWTIDHQAERATPDERAVLDLVVGRMT
                     DQCLAPATDFRPYSSSGFGTYGDPDGLARSLNYHLYASTGREAVLLANKDDEQSPLRF
                     VPTPLVP"
     gene            complement(397..1413)
                     /locus_tag="VFV30_02670"
     CDS             complement(397..1413)
                     /locus_tag="VFV30_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX4847023.1"
                     /translation="MIGRRAALATLGGLGAALIAPPVGAARSRKAPRLRPGDTVGVIA
                     PASAAGSEDTAARAEWWLKGMGLVPKIAEGADGRFGYLSAPDADRAADLNAMYADPEI
                     RAVFGLRGGWGSARILPLLDWSTIRRNPKLLIGFSDITALHLAFAARAGYPTIHGGNA
                     ASTWRPESWESLWRLAFAADRPVLGGAAAEAASGRSGRTIRGGVARGRLLGGNLTIVS
                     TLMGTGWLPSFKGAILFIEDVNEAEYRIDRMLQQLRLAGVLGELAGIAFGQCTSCRTE
                     DPGYRGFTLDEVIDQHLEPLGIPAFTGGNFGHITNQLSLPSGAMVELDADARTLRVLE
                     AAVA"
     gene            complement(1410..1838)
                     /locus_tag="VFV30_02675"
     CDS             complement(1410..1838)
                     /locus_tag="VFV30_02675"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX4847024.1"
                     /translation="MSWTRDSLIAAARDAASRAYAPYSNFHVGAALGFADGSVVTGAN
                     VENASYGLTLCAETVAVGKALSEDWRGRLEAVAVIGGKAGAVGSGDPVTPCGRCRQVL
                     NEVAALGGSDPTVWCVGEEQVTELLLSDLLPRAFGPGSLA"
     gene            complement(1835..2533)
                     /locus_tag="VFV30_02680"
     CDS             complement(1835..2533)
                     /locus_tag="VFV30_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEX4847025.1"
                     /translation="MARKSGNRWRLRAFGAVLLLAIIAAGWAWWDLRQWRPSKQEFPV
                     QGAEISGEDGAVNWKALKATGASFVYLDASASAFARDPAFAKNLEDARAAKLQVGAVH
                     KYDPCQPAERQAANFVTVVPRDARLLPPAVDLDMLADECPVKVTDSGVESELMTFINQ
                     IETHTGKPTILKISPAFEDRYHLANAIDRNVWLVRTRFQPDYAGRPWTLWSASDMFRS
                     EAAEAPLRWVVVQR"
     gene            complement(2550..>3000)
                     /locus_tag="VFV30_02685"
     CDS             complement(2550..>3000)
                     /locus_tag="VFV30_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX4847026.1"
                     /translation="DEATILWRNADIEQERRIAIFDLIEENLFKPLRAWEAGHQGPYR
                     LRLSVDDGRLSLVVTDADGGELETLILGLGRFRRPIREYFAICDSYYQAIRKATAAEI
                     ETIDMARRGVHNEAAEMLLERLEGKVETDFATARRLFTLICVLHIRG"
BASE COUNT          467 a         1054 c         1008 g          471 t
ORIGIN      
        1 gggcaccaag ggggtaggga caaagcgcag cggcgactgt tcatcgtcct tgttggcaag
       61 caggacggct tcgcggcccg ttgaggcgta cagatggtaa tttagggatc gagcaagacc
      121 gtccgggtcg ccgtaagtcc cgaaccccga gctgctgtaa ggccgaaagt ctgttgcagg
      181 cgcgaggcac tggtctgtca tccgccccac gaccaggtcc agcacggccc gttcgtccgg
      241 ggtggcccgt tcggcctgat ggtcgatcgt ccaccaaccg gtgaggccca gcaacagcac
      301 cgacagggct ctgcgtgtgt tcggcatcct gccgcccctc tcctgcgtta tgtaacaaca
      361 taacacgaat cggtgaggcc gcaagtccgc tggccgtcag gcgacagccg cctccagcac
      421 gcgcaaggtt cgcgcatcgg cgtccagctc caccatcgcg cccgatggca gggacagctg
      481 gttcgtgatg tggccgaaat tgccgccggt gaaggccgga atgcccagcg gttccagatg
      541 ctggtcgatc acctcgtcca gggtgaagcc gcggtagccg gggtcttccg tccggcagct
      601 ggtgcactgg ccaaaggcga tcccggccag ctcacccagc accccggcca ggcgcaattg
      661 ctgaagcatc cggtcgatcc ggtattcggc ctcgttgacg tcctcgatga acaggatcgc
      721 gcccttgaac gagggcagcc agccagtgcc catcagagtg gagacaatcg tcaggttccc
      781 gcccagcagg cggccgcggg ccacgccgcc gcggatcgtg cgcccgctcc gcccgctggc
      841 cgcttcggcc gccgcgccgc ccagcaccgg acggtcggcc gcgaaggcca gccgccatag
      901 gctttcccag ctttcgggcc gccaggtgct ggccgcgttg ccgccgtgga tggtcgggta
      961 tccggcccgc gccgcgaagg cgaggtgcag cgcggtgatg tcgctgaagc cgatcagcag
     1021 cttgggattg cggcggatcg tgctccagtc cagcagcggc aggatccgcg cgctgcccca
     1081 tccgccgcgc agcccaaaga ccgcgcggat ttccggatcg gcatacatcg cgttgagatc
     1141 ggcggcccgg tccgcatcgg gggcggagag atagccgaac cgcccgtccg cgccctcggc
     1201 aatcttcggg accagcccca tgcctttcag ccaccattcg gcgcgggctg cggtatcctc
     1261 gctgccggcg gcgctggccg gggcgatcac gcccaccgtg tcaccggggc gcagccgggg
     1321 cgctttccgg cttcgcgccg cgcccaccgg cggcgcgatc agcgcggcgc ccagcccgcc
     1381 cagcgtggcc agcgcggcgc gccgtccgat caagcgaggc tgcctggccc gaaggcgcgc
     1441 ggcagcaggt cggacaggag cagctcggtc acctgctctt cgcccacgca ccacacggtc
     1501 gggtcgctgc cgcccagcgc ggcgacttcg ttcagcacct ggcggcaccg gccgcagggg
     1561 gtgaccggat cgccgctgcc gaccgcgccg gccttgccgc cgatcaccgc cacggcttcg
     1621 agccgcccgc gccagtcctc gctcagcgcc ttgccgaccg cgaccgtttc cgcgcagagg
     1681 gtcaggccat aactggcgtt ctcgacattg gcgcccgtca ccacagagcc atcggcaaag
     1741 cccagcgccg cgccgacgtg aaagttggag tagggggcat aggccctgct ggccgcatcg
     1801 cgggcggcgg cgatcaggct gtcgcgggtc cagctcatcg ttgcaccacc acccagcgca
     1861 ggggggcttc ggcggcttcg cttcggaaca tgtcgctggc actccacagg gtccacggcc
     1921 gcccggcata gtccggctgg aagcgggtcc gcaccagcca gacgttgcga tcgatcgcgt
     1981 tggccagatg atagcggtct tcgaaggccg gggaaatctt caggatggtc ggcttgccgg
     2041 tgtgggtctc gatctggttg atgaaggtca tcaactcgct ctccaccccg gaatcggtca
     2101 ccttcaccgg gcactcgtcg gccagcatgt cgagatcgac cgccggcggc agcagccggg
     2161 catcgcgcgg caccacggtg acgaagttcg ccgcctggcg ctcggccggc tggcagggat
     2221 cgtacttgtg gaccgcgccg acctgcagct tggcggcgcg ggcgtcttcc aggttcttgg
     2281 caaaggccgg atcgcgggca aaggcgctgg ccgaggcatc gagatagacg aagctggcgc
     2341 cggtggcctt cagcgccttc cagttgaccg cgccgtcctc gcccgaaatc tccgcgccct
     2401 ggaccggaaa ctcctgcttc gacgggcgcc attggcgcag gtcccaccag gcccagccgg
     2461 ccgcgatgat cgcgagcaga agcactgccc cgaacgcgcg cagccgccag cggttgccgg
     2521 atttcctcgc catgtgaaaa agcgctccct taccccctga tatgcagcac gcagatcagg
     2581 gtaaagagcc gccgggcggt ggcgaagtcg gtttccactt tcccctccag ccgttccagc
     2641 agcatttcgg ccgcttcgtt gtggaccccg cggcgggcca tgtcgatggt ctcgatctcg
     2701 gcggcggtgg ccttgcggat cgcctggtaa taggaatcgc agatcgcgaa gtattcgcgg
     2761 atcgggcggc ggaaccggcc cagccccagg atcagcgtct ccagctcgcc cccgtcggca
     2821 tcggtgacaa ccagcgagag ccgcccgtca tcgacggata gccgcagccg gtaggggccc
     2881 tggtggccgg cctcccaggc gcgcagcggc ttgaacaggt tttcctcgat caggtcgaag
     2941 atcgcgatgc gccgttcctg ttcgatatcg gcgttgcgcc acaggatggt cgcctcgtcg
//
LOCUS       DASUDV010000122         8085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_754737,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000122 DASUDV010000000
VERSION     DASUDV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754737"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFV30_02690"
     CDS             complement(<1..185)
                     /locus_tag="VFV30_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX4847027.1"
                     /translation="MADSRTLKLEGVHNFRDYGGYDTVHGAPVRKGLLWRSAQHGDAS
                     DADLAVIHGLDLANVIDL"
     gene            complement(234..1505)
                     /locus_tag="VFV30_02695"
     CDS             complement(234..1505)
                     /locus_tag="VFV30_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX4847028.1"
                     /translation="MNTPTPRVAVIGLGYVGLPLALALARKFETWGIDVDRTRIAELR
                     DGHDRTGEVDSPILRASPIRLLEGLADCPPCDFYIVTVPTPIDPQNRPDLDLVVKASR
                     SVGAVLAAAAAEGRKPVVVYESTVYPGVTEDICAPVLEEVSGLRSGADFFLAYSPERI
                     NPGDREHTVDKITKVVSGQTPEVLERVSALYGAITSGGVFKASSIKVAEAAKVIENAQ
                     RDINIAFMNEIAQIFSRIGISTWDVLAAAGTKWNFLPFTPGLVGGHCIGIDPYYLAHR
                     ARELGIEPNVITAGRGINDSMAEWVAGELHTARGAKAGSVLVLGLTFKENIPDLRNSK
                     VADLAGALGRLGHAVTVHDPMADPDEARHEYGIELAATLPEGPFDLVLLAVPHSAYQD
                     LGVDALRGLLAPGGTLADLKGVLSGQGDWSL"
     gene            complement(1515..2771)
                     /locus_tag="VFV30_02700"
     CDS             complement(1515..2771)
                     /locus_tag="VFV30_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX4847029.1"
                     /translation="MRRKSAWLNPLTQLVLAALVCVGLPFLVTSLFQEDGLRDYVSHT
                     TVIACLAGLLIGFWLHRSLNKLPGTYESFGVLPGYLASFSLVLSAILLFRIEYSRSLL
                     IVSFVTCIVWFFMVYLVVQRKTELRLGLIDGGRVPQFDGLTGISSERLDLDTEIADFD
                     AVAADFRFAHSDAWEARIADYVLAGVPVYHSKDLFESLTGRTELESLSENNFGALGPA
                     HSWMGPKLVIDWLLAAVALPLLSPVLLLAALAIRLDSSGPILFRQTRVGYRGEEFTVF
                     KFRTMHNRRKGDGMATVDDLITRADDARITRVGRFLRFSRIDELPQLLNVLRGEMSVI
                     GPRPEAAGLSDWYQAEVPFYRYRHIVRPGITGWAQVNQGHVAGVDDIKMKLQYDFYYI
                     RNFSVWLDILIVMKTVKTMLTGFGHK"
     gene            2974..4161
                     /locus_tag="VFV30_02705"
     CDS             2974..4161
                     /locus_tag="VFV30_02705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4847030.1"
                     /translation="MVDAEARTAHRILILIPSLRSAGGTERVVLNLSRLLAGMGHTVK
                     TATFDAPEDKPHFAFDPPPMALGPLPALPFALRPLTYLLEALRLREAKRAFRPDLTIS
                     NLWRADLISQLAGGADRKLAVAHINVVGNPTNRMMVRLRPLVAWLYRRFDRVVAVSPP
                     LAEELRGLYRLAPGRALAIDNFTDPPPAQPALPDDGLARLVWCGRLVPEKNLGGFLPV
                     WAQFCRGRTGVQLVVLGEGPLRAALEAQAAELGLRAGSDPGDPNAQVVFAGVVSRPAD
                     YMIGAKALVLSSEAEGMPMVLLEAIALGTPVLASDCPAGGVRAVLSEDGAEATGFGAL
                     LPIPRAGEPASQQAWLPWLACAVEDPATQQDWHRAALLRAEKYSSRTAQQAWQAAIEG
                     ALA"
     gene            4158..5375
                     /locus_tag="VFV30_02710"
     CDS             4158..5375
                     /locus_tag="VFV30_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4847031.1"
                     /translation="MSVLYITQNGICDHIGQSQVAPYVLGLAAQGHAIHVLSAEKPGQ
                     DALVARYQALFDQAGVRWTRVPYRSAPKVIGPALTQWQLERAARRIIADEPVHAVHCR
                     SFPAALIGHRLKRSLGVPYLFDFRDFYADGGLTKGARVLRPLYARMKRMEGPMIRDAA
                     RVVCLTERARDLLVDWYLKDVPGAADRFTVIPCCADFRHFDPARVEAEHMAEARQRLG
                     LADGQTVLLYLGSLGPDYLLPEMIRLFAALLDLRSDARFLFVSNNGRELVEAECRAQG
                     VPADRIVFTSVGRDAVPTLIALATLSVVFIRADLSKAGCSPTKLAELFAMNVPVIANR
                     GVGDMDRIVSPDRNGSTLLADFSSDAMRRAIAEVLEMRENGARAIRENSAEFDLPAGV
                     AAYAKVYRELEAG"
     gene            5375..6601
                     /locus_tag="VFV30_02715"
     CDS             5375..6601
                     /locus_tag="VFV30_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX4847032.1"
                     /translation="MLNVVVLNGGRGAATLIPALLAQEGLRVTSVVNAYDDGKSTGEI
                     RRFFGMLGPSDIRKVQELMLRENDPDYAANLNLFAYRYPLDCDRDTVIADLRAFAEGR
                     RDDVVGSVFSTATVRAALQVFVRDFLDGLAIIEKVVPERFSFADCSIMNCIYAGAFLH
                     FNRNIEQATMFISRLFKLRGTVLPNSIEDKKLTALREDGEMLYCEAEIVELRSNVRIE
                     RIYLLDQPPARERFEPLSRAEKRAYLERHHCFVETTPHVQLALQQADVIIYSAGTQHS
                     SLYPTYLTTGLARAIADNRAAFKVFVTNIGADYETPSYVASDYLRGAYRYLNLGDARD
                     YALNELFDRVLINTGRRKGDETYVEFDAAGFADLDVAQDLADFESAEAPGRHDGAKLV
                     ATILERFEQASPGLRA"
     gene            6598..7221
                     /locus_tag="VFV30_02720"
     CDS             6598..7221
                     /locus_tag="VFV30_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4847033.1"
                     /translation="MTPGPELARLLAGRTLLIFDFDGTIADSSPLHAQAFTQVLEPLG
                     IPVDYPRLAGRSTRDAVLHSFALAGQPEPESALVEELVRQKQALGRELIASSLMALPA
                     AAALLDWARSRFRLALVSSGSRGTIDIALGRLGLADWFDPLITADDVAQAKPDPQGFL
                     LALDRARVAAEAALIFEDAESGFEAARRAGVTAVDARRLDSLMVTES"
     gene            7222..8076
                     /locus_tag="VFV30_02725"
     CDS             7222..8076
                     /locus_tag="VFV30_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847034.1"
                     /translation="MGIDLHTFRFMQGLARSRPLGRVLTIGRQNVDFDPALAGAAPAE
                     PDRARYCEWAFEALGAETVSSVDVSDYENATYVADLGQPTDLPQQFDTVIDAGSLEHV
                     FDVVTAFRNVIGFTAIGGRIVHVLPVNNLSGHGFWQFSSDLLYALYAPNNGFGETRVY
                     YASGLNPDAWHLVPEPHPGTRTELVSLEPVILLSVTTKLRAVDSLSVMQPFYAKAWEG
                     TEAVQSKPASPSPVRQAAKRLRGTAAYRLARNAYTVAGLLFGTSRYALSGSRFARRSP
                     SSGESGPP"
BASE COUNT         1315 a         2712 c         2682 g         1376 t
ORIGIN      
        1 aggtcgatca cgttggccag gtccaggccg tggatcaccg ccaggtcggc atcgctggca
       61 tcgccgtgct gggccgagcg ccacagcagc cccttccgca cgggcgcgcc gtggacggta
      121 tcgtaaccgc catagtcgcg gaaattgtgg acgccttcga gcttcagggt gcgggaatca
      181 gccatgccct gccgcttggc agcttggccg gatccgcgca acgggtttga ggcctagagc
      241 gaccagtccc cctgcccgga cagcacgccc ttgagatcgg ccagggtgcc cccgggggcc
      301 agcagtccgc gcagggcgtc cacaccgaga tcctgatagg cgctgtgcgg caccgcgagc
      361 aggaccagat cgaacggccc ttcgggcagc gtcgcggcca gctcgatccc gtattcgtgc
      421 cgcgcctcgt ccggatcggc catcggatcg tggacggtca cggcgtggcc cagccggccc
      481 agcgccccgg ccaggtcggc gaccttgctg ttgcgcaggt cggggatgtt ttccttgaag
      541 gtcaggccca gcaccagcac cgagccggcc tttgcgccgc gcgccgtgtg caactcgccg
      601 gccacccatt cggccatgga atcgttgatc ccccgccccg cggtgatcac gttgggttcg
      661 atccctaatt cgcgggcgcg gtgggccagg tagtagggat cgatcccgat gcagtgcccg
      721 ccgaccagcc ccggggtgaa gggcaggaaa ttccacttgg ttccggccgc ggccaggacg
      781 tcccaggtgg agatcccgat ccgcgaaaag atctgggcga tctcgttcat gaaggcgatg
      841 ttgatgtcgc gctgggcgtt ctcgatcacc ttggcggcct cggccacctt gatcgagctg
      901 gccttgaaca cgccgccgct agtgatcgcg ccgtaaagcg cggacacgcg ctccagcact
      961 tcgggggtct ggccggagac gaccttggtg atcttgtcga cggtgtgctc ccggtcgccc
     1021 ggattgatcc gctccgggct gtaggcgagg aagaaatcgg ccccgctgcg caggcccgaa
     1081 acctcttcca gcaccggcgc gcagatgtcc tcggtcacgc ccggatagac ggtgctttca
     1141 tagaccacga ccggcttgcg cccctcggcc gcggcagccg cgaggacagc gccgaccgag
     1201 cggctcgcct tgaccaccag atccaggtcg ggccggttct gcggatcgat cggggtcggc
     1261 acggtgacga tatagaaatc gcacggcggg caatcggcca gcccttcaag caggcggatc
     1321 gggctggcgc gcaggatcgg gctgtcaacc tcgccagtcc ggtcgtgccc gtcgcgcagt
     1381 tcggcaatcc gggtgcgatc gacatcgatc ccccaggttt caaacttgcg cgccagggcc
     1441 agcgccagcg gcaggccgac atagcccagg ccgatcacgg cgacgcgggg agtaggagtg
     1501 ttcatcagga aaccttactt gtggccaaag ccggtcagca tcgtcttcac cgtcttcatc
     1561 acgatcagga tgtcgagcca gaccgagaag ttgcggatgt agtagaagtc gtactgcagc
     1621 ttcatcttga tatcgtcgac cccggccacg tggccctggt tgacttgcgc ccagccggtg
     1681 atccccggac ggacgatgtg gcgatagcga tagaacggaa cctccgcctg ataccagtcg
     1741 gaaagccccg cggcttcggg acgcgggccg atcacgctca tttcgccgcg aaggacgttg
     1801 aggagctgcg gcaattcgtc gatccgcgag aagcgcagga accggccgac ccgcgtgatc
     1861 cgcgcgtcgt cggcccgggt gatcagatcg tcgaccgtcg ccatcccgtc gcccttgcgg
     1921 cggttgtgca tggtccggaa cttgaagacc gtgaactctt ccccgcgata gccgacgcgg
     1981 gtctggcgga acaggatcgg gccggaactg tccagccgga tcgccagcgc ggccagcagc
     2041 agcaccgggg acagcagcgg cagcgccacc gcggccagca gccagtcgat caccagcttg
     2101 ggccccatcc acgaatgcgc cggccccagc gcgccgaagt tgttttcgga gaggctttcc
     2161 agctcggtgc ggccggtcag cgattcaaac aggtccttgg aatggtagac gggaactccg
     2221 gccaggacgt aatcggcaat ccgggcttcc caggcatcgc tgtgcgcgaa ccggaaatcg
     2281 gcggccacgg catcgaaatc cgcgatctcg gtatccagat ccagccgttc gctggaaatg
     2341 ccggtcaggc catcgaactg cggcacccgg ccgccgtcga tcaggcccag ccgcagctcg
     2401 gttttacgct ggaccaccag gtagaccatg aagaaccaga cgatgcaggt gacaaagctg
     2461 acgatcagca gcgagcggga atattcgatc cggaacagca ggatcgccga aagcaccagg
     2521 ctgaacgagg cgagatagcc cggcaggacg ccaaagcttt cataggtgcc cggcagcttg
     2581 ttgagcgagc ggtgcagcca gaaaccgatc agcaggccgg ccaggcaggc gataaccgtg
     2641 gtatgcgata catagtcgcg caggccatcc tcctgaaaca ggctggtcac caggaaaggc
     2701 aggccgacac agaccagcgc cgccaggacc agttgggtca gcggattcag ccatgcagac
     2761 ttgcggcgca tacgaaggcg gtttggtgcc atgcaatcgc gacctagcgt ttcccagcac
     2821 agttcgggtc cgacggaaga tcggactggt aagcccgcct aaggcggcat aaagggtttg
     2881 tcatgtctaa atcggtcaac gcattcgtat tgctgaattt ctttgcggat actgtcgctt
     2941 ggctgaacaa gggctgtcaa acggacccgt gccatggttg atgccgaagc gcgcaccgcg
     3001 caccggatcc tgatcctgat cccttcgctg cgcagcgccg gcgggacgga acgggtcgtg
     3061 ctcaacctct cgcgcttgct ggccggcatg ggccacacgg tgaaaaccgc cactttcgac
     3121 gcgccagagg acaagccgca cttcgccttc gatccgccgc cgatggcact gggcccgctg
     3181 cccgccctgc cctttgcgct acgcccgctg acctatctgc tggaagcgct gcggctgcga
     3241 gaggccaagc gcgcgttccg gccggacctg acgatcagca acctgtggcg cgcggacctg
     3301 atcagccagc tggccggcgg cgcggaccgc aagctggccg tcgcgcacat caacgtggtc
     3361 ggcaacccga ccaaccggat gatggtgcgg ctgcgcccgc tggtcgcctg gctctaccgc
     3421 cggtttgacc gggtcgtggc ggtcagtccg ccgctcgcgg aggaactgcg cgggctctat
     3481 cgccttgccc ccggccgggc cctcgccatc gacaatttca ccgatccgcc cccagcccag
     3541 ccggccctgc cggatgacgg cctggcccgg ctggtctggt gcggacggct ggtgccggaa
     3601 aagaacctgg gcggtttcct gccggtctgg gcgcaattct gccgtggccg cacgggcgtg
     3661 cagctggtgg tcctgggcga aggaccgctc cgcgccgcgc tcgaagcgca ggcggcggag
     3721 ctgggcctgc gcgccggttc ggacccgggc gacccgaacg cgcaggtggt ctttgccggt
     3781 gtcgtatccc gcccggcaga ctatatgatc ggcgcgaagg ccctggttct gagttccgag
     3841 gccgagggaa tgccgatggt tcttctggaa gcgatcgcgc ttggcacccc ggtgctggct
     3901 tcggattgtc cggccggcgg cgtgcgcgcg gtgctgagcg aggacggcgc ggaagcaacc
     3961 gggttcggag cgcttctgcc catcccgcgt gcgggggagc cggccagcca gcaggcctgg
     4021 ctcccctggc tggcctgcgc ggtcgaggat cccgccacgc agcaggactg gcaccgggcg
     4081 gctctgctcc gcgccgagaa gtactcgtcc cgcaccgccc agcaggcctg gcaggccgcg
     4141 atcgaaggcg cgctggcatg agtgtgctct acatcaccca gaacggcatc tgcgaccata
     4201 tcggccagtc gcaggtcgcg ccctatgtcc tcggcctcgc ggcgcagggc catgcgatcc
     4261 acgtcctcag cgcggaaaag cccgggcagg acgcgctggt ggcgcgttat caggcgctgt
     4321 tcgatcaggc cggggtgcgc tggacccggg tgccctaccg ctccgcgccc aaggtgatcg
     4381 gcccggcgct gacccagtgg cagctggaac gcgcggcccg gcggatcatc gcggacgagc
     4441 cggtccatgc cgtgcattgc cgcagctttc ccgccgcgct gatcggccac cggctgaagc
     4501 gcagtctggg cgtgccctac ctgttcgact ttcgcgattt ctatgccgat ggcgggctga
     4561 ccaagggcgc gcgggtgctg cgcccgctct atgcccggat gaagcggatg gaaggcccga
     4621 tgatccgcga tgccgcgcgg gtcgtgtgtc ttacggagcg ggcgcgcgac ctgctggtcg
     4681 actggtatct gaaggacgtg cccggcgcgg ccgaccgctt taccgtcatc ccgtgctgcg
     4741 ccgatttccg ccatttcgat cccgcccgcg tcgaggcgga gcacatggcc gaggcccgcc
     4801 agcgcctcgg cctggcggat gggcaaaccg tgctgctcta ccttggcagc ctgggaccgg
     4861 actatctcct gcccgagatg atccgcctgt tcgccgccct gctcgatctg cgaagcgacg
     4921 cacggttcct gttcgtgtcg aacaacggcc gcgagctggt ggaagcggag tgccgcgcac
     4981 agggtgtgcc ggcggaccgg atcgtcttca cctcggtcgg ccgcgacgcg gtgccgaccc
     5041 tgatcgcgct tgccactctc tcggtcgtgt tcatccgggc cgacctgtcc aaggccgggt
     5101 gttcgcccac caagctggcc gaactctttg cgatgaacgt gccggtgatc gccaaccgcg
     5161 gcgtcggcga catggaccgg atcgtttcac cggatcgcaa cggatcgacc ttactcgctg
     5221 atttttcgag cgatgcgatg cgccgggcga ttgccgaagt gcttgaaatg cgcgagaatg
     5281 gggcgcgcgc cattcgcgag aacagtgcgg aattcgacct gccggcgggt gttgctgcct
     5341 atgccaaggt ctatcgggaa ctggaagctg gctgatgctg aatgtggtgg tgttgaacgg
     5401 cggccgcggg gctgccaccc tgatcccggc cctgctggcc caggaagggc tgcgcgtcac
     5461 ttcggtggtc aacgcctatg acgatggcaa gtcgaccggc gagatccgcc gcttcttcgg
     5521 catgttgggc ccgtcggaca tccgcaaggt gcaggaactg atgctgcggg aaaacgatcc
     5581 cgattacgcg gccaatctca acctgttcgc ctatcgctat ccgctcgatt gcgaccgcga
     5641 cacggtgatc gccgatctgc gcgcctttgc cgaggggcgc cgcgacgatg tggtcggttc
     5701 ggtcttttcc accgccacgg ttcgcgccgc cctgcaggtt ttcgtgcgcg attttctgga
     5761 cgggctggcg atcatcgaga aggtcgtgcc cgagcggttc tctttcgccg actgctcgat
     5821 catgaactgc atctacgcgg gcgcgttcct gcacttcaac cgcaacatcg aacaggccac
     5881 catgttcatc agccgcctgt tcaagctgcg cggcacggtc ctgcccaatt cgatcgagga
     5941 caagaagctt accgcgctgc gcgaagacgg ggaaatgctg tattgcgagg ccgagattgt
     6001 cgagcttcgc tccaacgtcc ggatcgagcg gatctacctg ctggatcagc cccccgcgcg
     6061 ggagcggttc gagccgctca gccgggcgga aaagcgcgcc tacctcgaac ggcaccactg
     6121 cttcgtggaa accactccgc acgtgcagct ggcgctgcaa caggcggacg tgatcatcta
     6181 ttcggccggg acgcagcact cctcgctcta tcccacctac ctcaccaccg gcctggcccg
     6241 cgcgatcgcg gacaaccgag cggccttcaa ggtgttcgtg accaacatcg gggcggacta
     6301 tgaaacgccc agctatgtcg cgtcggacta tctgcgcggg gcctatcgct atctcaacct
     6361 cggcgatgcg cgcgactatg ccctgaacga gctgttcgac cgggtcctga tcaacaccgg
     6421 ccgccgcaag ggcgacgaga cctatgtcga attcgacgcg gccggttttg ccgatctgga
     6481 cgtcgcgcag gacctggccg atttcgaatc ggccgaagcc ccgggccgcc atgacggcgc
     6541 caagctggtc gccaccattc tggaacggtt cgagcaggcc agcccgggcc tgcgcgcgtg
     6601 acccccggtc cggaactggc gcgcctgctt gcaggccgga cgctgcttat cttcgatttc
     6661 gacggaacga ttgccgacag ttcgccgctg cacgcgcagg cctttaccca ggtgctggag
     6721 ccgctcggca ttccggtgga ctacccgcgc ctcgccgggc gctcgacccg cgatgcggtg
     6781 ctgcacagct ttgccctcgc cggacagccc gaaccggaga gcgcgctggt ggaagagctt
     6841 gtccgccaga agcaggcgct ggggcgggag ctgatcgcca gcagcctgat ggccctgccc
     6901 gctgccgctg ccctgctcga ctgggcgcgc agccgcttcc ggctggcgct ggtcagttcc
     6961 ggatcgcgcg gcacgatcga tatcgcgctg ggccggctgg gtctggccga ttggttcgac
     7021 cccctgatca ccgccgacga tgttgcgcag gccaagcccg atccgcaggg tttccttctg
     7081 gcgctggatc gcgcaagggt ggcggccgaa gccgcgctga ttttcgagga tgccgaatcc
     7141 ggtttcgagg cggcccgccg cgccggagtg accgcagtgg atgcccgcag gctggacagc
     7201 ctgatggtga cggagagttg aatgggcatc gatctgcata ccttccgttt catgcagggt
     7261 ctggcccgca gccgcccgct gggccgggtg ctgacgatcg ggcgccagaa cgtcgatttc
     7321 gatcccgccc tggccggcgc cgccccggcc gagccggacc gggcccgcta ctgcgaatgg
     7381 gcctttgaag cgctgggcgc ggagacggtc tcgtcggtcg atgtctcgga ctatgaaaac
     7441 gccacctacg tggccgacct gggccagccg accgacctgc cgcagcagtt cgatacggtg
     7501 atcgacgcgg gctcgctgga acacgtgttc gatgtcgtga ccgcgtttcg caacgtgatc
     7561 ggctttaccg cgatcggcgg ccggatcgtc catgttctgc cggtcaacaa cctcagcggg
     7621 cacggcttct ggcagttctc gtccgacctg ctctacgcgc tctacgcgcc gaacaacggc
     7681 tttggcgaga cccgggtcta ttatgcctcg ggcctcaatc ccgatgcctg gcacctggta
     7741 cccgaacccc atccgggaac gcggaccgag ctggtctcgc tcgaaccggt gatcctgctc
     7801 agcgtcacca ccaagctgcg cgcggtggac agcctgtcgg tgatgcagcc gttctacgcc
     7861 aaggcctggg aaggaaccga ggcggtgcag agcaagccgg cctccccctc cccggtccgc
     7921 caggcggcaa agcggctgcg cgggaccgcg gcctaccgcc tggcccgcaa cgcctatacc
     7981 gtggccggac tgctgtttgg caccagccgc tatgccctgt cgggatcgcg gttcgcccgg
     8041 cggtcgccgt cctcgggcga gtccggcccg ccatgaagat cctgg
//
LOCUS       DASUDV010000123         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1218856,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000123 DASUDV010000000
VERSION     DASUDV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218856"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFV30_02730"
     CDS             complement(<1..793)
                     /locus_tag="VFV30_02730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4847035.1"
                     /translation="MSVTLTPAQETARYIDGLGPEALAKAQAYTMGNHGLLLAGVLVS
                     VVLAWLFVRTGILDRIARRLEGKRWALATFLTATAFFAIADVIQLPWTIYTDWSRERA
                     YGMTQQPLGDFLGQWALSEGIGAVTGGLFMLGIYALIRKSGARWWIWATGFTGVVTAI
                     MLLAAPVVIEPLFNEYKPVPPGPVRTALEKIADDVEIPHDRIFMYDGSRQSENFTANV
                     SGIGPSARIAISDVALKEASLDEVKAVTGHEAGHYKLGHVWRYVVV"
     gene            complement(861..1568)
                     /locus_tag="VFV30_02735"
     CDS             complement(861..1568)
                     /locus_tag="VFV30_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847036.1"
                     /translation="MTSAAGAWNKDRGTRFYRFGALTAIAVALAGFFLTYLQPMAQGR
                     FSGPVEAHVHGMLLASWLVLVCVQSCLLRSHRTLGWLAVALAPAIALSTMAIGVAATR
                     RDLAAGIELGMAGNVTAPLIFCGLVAAAIALRRQPQWHKRLIFIATVAMLWPAWFRWR
                     HFLPWVPRPDIVLGLVVANLPVAVAMLRDRLKFGVIHPAYFWVGLPVVAEQTFETMVF
                     GTPLWTGFGMYLFRILG"
     gene            complement(1565..2293)
                     /gene="dapB"
                     /locus_tag="VFV30_02740"
     CDS             complement(1565..2293)
                     /gene="dapB"
                     /locus_tag="VFV30_02740"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX4847037.1"
                     /translation="MARIGIIGAKGRMGGALVDAIAAAGEELAGGVDKGDDVAALARA
                     CDVLVDFSSPHALEANLDAAIAAAVPIVVGTTGLEERHHWLCDHAAESVAVLQTGNTS
                     LGVTLLAHLVREAASRLGEDWDIEIAETHHRMKVDAPSGTALLLGEAAARGRGIDLAS
                     HAERGRDGITGARSGGAIGFASLRGGSVAGDHTVHFLADDERIALTHLAENRRIFARG
                     AVRAAQWLLDQAPGRYTMGEVLGL"
     gene            complement(2312..>3680)
                     /locus_tag="VFV30_02745"
     CDS             complement(2312..>3680)
                     /locus_tag="VFV30_02745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX4847038.1"
                     /translation="AVLVMATLVSLGWAWLKQADSDGFAFYLLPTRAWELFFGALLAL
                     GAVPPIRAAWLREGLAAAGLGLIAFAVASYDSQTVFPGLAALVPVLGSALLIHAAPGT
                     LVGRLLSNPLPVGIGLISYSLYLWHWPLIVFAEYARDAKLAGAWQVGVIAASIALAWA
                     SWRFVERPFRDPARFSQRRIFVLSAGAMAGLGAVAAAMLPLGGWPQRFDAQVVRYAAA
                     KDDFSPKRAACLTDAVGGERRECTIGAPGAAPAALLWGDSHGVELAWALGQEHGARGE
                     ALMQRTRGSCPPLSGLDRPKDPGCARFNAEVLERIRATPSLKTIYLAGYWDEHRDDPQ
                     TIARLDATIAALQAAGRRVVVIGPVPTQEWNVPRRLARLAMFGQADGATGASEAAYRQ
                     HNAWFLSAYPRWQAQGVGLIDPALALFVSGKGRIVKAGDPLYFDSHHLSVAGARAVLA
                     ADPRR"
BASE COUNT          686 a         1299 c         1185 g          510 t
ORIGIN      
        1 gcaccaccac atagcgccag acatggccca gtttgtaatg tccggcctcg tgcccggtca
       61 cggccttgac ctcgtcgagc gaggcttcct tcagcgccac gtcggaaatc gcgatccgcg
      121 cgctggggcc gatcccggaa acgttagcgg taaaattctc gctctggcgc gacccgtcgt
      181 acatgaagat ccggtcgtgc gggatctcga catcatcggc gatcttctca agcgccgtgc
      241 gcaccgggcc cggcggcacg ggcttgtatt cgttgaacag cggttcgatc accaccggcg
      301 cggccagcag catgatcgcg gtgaccaccc cggtaaagcc ggttgcccag atccaccagc
      361 gcgcacccga cttgcggatc agcgcataga tcccgagcat gaacagaccg cccgtcaccg
      421 cgccgatccc ctcgctcagc gcccattgcc caaggaaatc gcccagcggc tgctgggtca
      481 tgccataggc gcgctcacga ctccaatcgg tgtagatcgt ccagggcagc tggatcacgt
      541 cggcgatcgc gaaaaaggcc gtggcagtga ggaaggtggc caaggcccag cgcttgccct
      601 caaggcgcct ggcgatccga tcgagaatgc cggtgcgcac gaacagccag gccagcacca
      661 ccgaaaccag cacgcccgcg agcagcaggc cgtgattgcc catggtatag gcctgcgcct
      721 tggccagggc ttcaggcccc aggccatcga tatagcgcgc ggtttcctgc gccggagtga
      781 gggtgacgga catcaaaatt ccccgtttgg tgtattgtct agataataca caaccgttct
      841 ggactggcaa tgcgataatt tcagcccaga attcggaaaa ggtacatgcc aaatccggtc
      901 cacaatggtg ttccgaatac catggtttcg aaagtctgct cggcaaccac cggcaggcca
      961 acccagaagt aggccgggtg gatcacgccg aacttcaatc gatcgcgcag catggcaacc
     1021 gcgactggca ggttggccac gacaagcccc agcacaatgt cggggcgcgg tacccagggc
     1081 agaaaatgcc gccagcggaa ccaggccggc cagagcatcg caaccgtggc aatgaagatc
     1141 aggcgcttgt gccattgcgg ttggcggcgc agcgcaatgg ccgcggcgac aaggccgcaa
     1201 aagatcagcg gcgcggtgac attgccggcc atgccaagct cgatccccgc agccagatca
     1261 cgccgggtgg cggccacacc gattgccatg gtgctgagcg cgattgcggg cgccaacgca
     1321 accgccagcc agccaagtgt gcggtgcgat cgcagcaggc acgactggac gcaaaccagg
     1381 accagccagc tcgccagcag catgccatgg acatgggcct cgacggggcc ggaaaaccgt
     1441 ccttgcgcca ttggctggag ataggtcagg aaaaacccgg ccaaggccac cgcgatcgcc
     1501 gtcagcgcgc caaaccggta gaagcgggtg ccgcggtcct tgttccaggc ccccgcggcg
     1561 ctagtcacag tcccagcacc tcgcccatgg tgtagcgccc cggggcctgg tccagcaacc
     1621 actgcgccgc gcgcaccgcg cccctggcga agatccggcg gttctctgcc agatgggtca
     1681 gcgcgatgcg ctcgtcatcg gccaggaagt gaaccgtgtg atcgcccgcc acgctgccgc
     1741 cgcgcaagga cgcaaagccg attgccccgc ctgagcgcgc gccggtgatc ccgtcgcggc
     1801 cgcgttcggc atggctggcc agatcgatcc cgcgcccgcg cgccgccgcc tcgcccagca
     1861 ggagtgcagt gcccgatggt gcatcgacct tcatccggtg atgggtttcg gcgatctcga
     1921 tgtcccagtc ttcgcccagc cggctcgccg cctcgcgcac caggtgggcc agcagggtga
     1981 cgccaagcga agtattaccg gtctgcagca cggccacgct ttcggcggca tgatcgcaca
     2041 gccagtggtg gcgttcttct aggccggtgg tgccgaccac gatcggcacg gcggcggcga
     2101 ttgccgcatc gaggttcgcc tcaagcgcgt gggggctcga aaaatcgacc agcacgtcgc
     2161 aggcccgagc cagcgccgca acatcgtcgc ccttgtcgac cccgccagcc agttcttcgc
     2221 cggcggccgc gatcgcatcg accagcgcgc cgcccatgcg ccccttcgca ccgatgatgc
     2281 cgatccgtgc catgccttgc ccgccccttg cctagcggcg gggatcggct gccagtacgg
     2341 cccgggcccc cgccacgctg agatgatgcg aatcgaagta gagcggatcg cccgccttca
     2401 caatccgccc cttgccagac acgaacaggg cgagcgcggg atcgatcagc cccacccctt
     2461 gcgcctgcca gcgcggatag gcggaaagga accaggcgtt gtgctggcga taggcggctt
     2521 cggacgcacc ggtggccccg tcagcctggc cgaacatggc cagccgggcc agacggcgcg
     2581 gcacgttcca ctcctgggta gggaccggac cgatcaccac cacccggcgc ccggcggcct
     2641 gaagcgcggc gatggtcgca tcgagccggg cgatggtctg cggatcgtcg cggtgttcat
     2701 cccagtagcc ggccaggtag atcgtcttga gcgatggagt ggcgcggatc cgttcgagca
     2761 cttcggcatt gaagcgcgcg caacccggat ccttgggccg gtcgagcccg ctgagcggcg
     2821 ggcacgagcc gcgggtgcgc tgcatcagcg cctcaccgcg cgctccatgc tcctgcccca
     2881 gcgcccaggc cagttccacc ccgtggctat cgccccacag cagcgcggca ggcgcggcgc
     2941 cgggcgcgcc gatcgtgcat tcgcggcgct cgccgcccac cgcgtcggtc aggcaagccg
     3001 cgcgcttggg gctgaaatcg tccttggctg cggcatagcg gaccacctgc gcatcaaagc
     3061 gctgcggcca gccacccagc ggcagcatcg ccgccgccac cgcgccaagg cccgccatgg
     3121 cgccagcgct cagcacaaag atgcggcgct ggctgaaccg cgcgggatcg cggaacggac
     3181 gctcgacaaa gcgccacgag gcccaggcca gtgcaatcga ggcggcgatc acgcccacct
     3241 gccaggcccc ggcaagcttc gcgtcgcggg catattcggc aaagacgatc agcggccagt
     3301 gccacaggta gagcgaatag gagatgagcc cgattcccac cggcagcggg tttgacagca
     3361 ggcggcccac cagcgtgccg ggcgcggcgt gaatcagcag cgctgaaccc agcaccggga
     3421 ccagcgcggc aaggccggga aacaccgtct ggctatcgta gctggcaacg gcaaaggcga
     3481 tcaggcccag ccccgcagcg gccagccctt cgcgcagcca ggcggcgcgg atcggcggca
     3541 ccgcgcccag cgccagcagc gcgccgaaga acagctccca ggcccgggtc ggcagcaggt
     3601 agaaggcaaa gccatcgcta tcggcctgct tcagccaggc ccagcccagc gaaaccagcg
     3661 tcgccatcac cagtactgcg
//
LOCUS       DASUDV010000124         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_887329,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000124 DASUDV010000000
VERSION     DASUDV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887329"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1375
                     /locus_tag="VFV30_02750"
     CDS             68..1375
                     /locus_tag="VFV30_02750"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX4847039.1"
                     /translation="MKDFATSDSPRVQAQLDRLAALSLPSGRLGLEVVRELLARLGNP
                     QDRLPPVLHVAGTNGKGSTCAYLRAILEADGYTVHAATKPHLVRYNERIRLAGKLIED
                     DLLADLLKEALDAGEDLAPSFFEVTTAAMFMGFSRVPADVCVIEVGLGGRFDATNVLD
                     HQAACAIATLGIDHEAFLLVPDEGVPSQPMCRIAFEKAGIARAGVPLVTQNYAADVAA
                     TIQAHADAVGARAVMRGRDWFADVGAEIAYRDTAGELAMPLPSLAGTHQADNAALAVA
                     MLRHQSALTVSPQAMARGIRAARWPARLQLLGEGPLTALVPGRRVWLDGGHNVDAGLA
                     IARHFADAEPFHLITGMLTSKDPSAIVSPLEGRLASLSVVPAPGHEAHRPEDFAPFTA
                     LPVRSFSEVASALLALPPEGDVLIAGSLYLAGEVLRLNQEFPD"
     gene            complement(1372..>2624)
                     /locus_tag="VFV30_02755"
     CDS             complement(1372..>2624)
                     /locus_tag="VFV30_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847040.1"
                     /translation="TLLVATMTFWLAKVGIDTATIGFAVALGTPYTIKFLWAPLVDKL
                     PLPVLTRLFGQRRGWLFFVQALLFVAIWQLGASDPQTGHMGPFIFWAIVTAFLSATQD
                     IVIDAYRIEILEEDEFAHGTATNQFGYRTGNLIAGAGTVYLASTEGLGLGWAMGYGLT
                     ALCIIPAIAGAIWAGPGKFIDRYAEAAGMSAGQWLKETVYNPFREFLGRHGAFLILAF
                     VLVYKVGDAMGQTMLGPMIVDLGFADLDYVSANKIWGFAALIVGSALGAPFILWLGMG
                     RALLISGLLMMVSNVMFMILAVVGNSYWMLVLAIVTENMTSGIGLTVFTTYLSGLSSL
                     AYTATQFALLSSFAAFGRTWLASPAGVIADKLGWTAFWGFTVVAAIPGMLLLWLLWNK
                     GYLVQSIRQPSTEDDGHAPTPVKT"
BASE COUNT          478 a          857 c          862 g          427 t
ORIGIN      
        1 gcgagctgaa ggcgacgctg ggcaagctgc tgggctatct ggaccgtaaa gaggcggcct
       61 gagccgcgtg aaagacttcg ccacatccga tagtccgcgc gtccaggcgc agcttgaccg
      121 gctggccgcg ctgagcctgc cgagcgggcg gctggggctg gaagtggtgc gcgaactgct
      181 cgcccggctg ggcaatccgc aagaccgcct gcccccggtg ctgcacgtgg cgggaaccaa
      241 cggcaagggc agcacctgcg cctatctgcg cgctatcctt gaggccgatg gttataccgt
      301 ccacgccgcc accaagccgc acctggtgcg ctataatgag cggatccgcc ttgccggcaa
      361 gctgatcgag gatgatctgc ttgccgacct gctgaaggaa gcgcttgatg ccggggagga
      421 cctggccccc agcttcttcg aggtgaccac cgcggcgatg ttcatgggct tttcgcgcgt
      481 tcccgccgat gtctgcgtga tcgaggtggg cctgggcggg cgcttcgatg ccaccaacgt
      541 gctcgatcac caggccgcct gcgccatcgc cacgctgggg atcgatcacg aagccttcct
      601 gctggtgccc gacgaaggcg tacccagcca gccgatgtgc cggatcgcct tcgaaaaggc
      661 cgggatcgcg cgcgccgggg tgccgctggt gacgcagaac tatgccgccg atgtcgcggc
      721 aacgatccag gcccacgccg atgccgtggg tgcgcgcgcg gtgatgcgcg ggcgcgactg
      781 gtttgccgat gtcggggccg agattgccta ccgcgatact gcgggcgaat tggccatgcc
      841 cctgcccagc cttgccggaa cccaccaggc cgacaacgcc gcgctggcag tggccatgct
      901 gcgccaccag tcggcgctga cggtttcacc ccaggccatg gcccggggga tccgcgccgc
      961 gcgctggccg gcgcggctgc aactgctcgg cgaaggcccg ctgaccgcgc tggtgccggg
     1021 gcggcgggtg tggctggacg gcgggcacaa tgtcgatgcg gggctggcga ttgcccggca
     1081 ttttgccgat gcagagccat ttcacctgat tacgggcatg ctgaccagca aggatccctc
     1141 ggcgatcgtc tcgccgctcg aaggccggct ggcctcgctg tcggtcgttc ccgcgccggg
     1201 ccatgaagcc cacagacctg aggatttcgc tcctttcacc gccttgccgg tgcgctcttt
     1261 ctccgaagtt gcttcggcgc tgctcgcgct tccgcccgaa ggtgacgtgc tgatcgccgg
     1321 atcgctctat cttgcaggcg aagtgctgcg cctgaaccag gagtttccgg actaggtctt
     1381 gacgggcgtt ggcgcgtgcc catcgtcctc ggtgctgggc tggcggatcg actggaccag
     1441 atagcccttg ttccacagca gccacagcag cagcatcccg ggaattgccg ccacaacggt
     1501 gaagccccag aaggcggtcc agcccagctt gtcggcgatc accccggcgg gcgatgccag
     1561 ccaggtccgc ccgaaggcgg cgaaggagga aagcagcgcg aactgcgtgg cggtataggc
     1621 cagactggaa agaccggaga ggtaggtggt gaacacggtc agaccgatcc cgctggtcat
     1681 gttctcggtc acgatcgcga ggaccagcat ccagtagctg ttgcctacca cggccaggat
     1741 catgaacata acgttcgaga ccatcatcaa gaggcccgag atcagcagcg cccggcccat
     1801 gcccaaccac aagatgaacg gcgcacccag cgccgatccc acgatcagcg cggcaaagcc
     1861 ccagatcttg ttggccgaga catagtcgag atcagcaaag cccagatcga cgatcatcgg
     1921 ccccagcatg gtttggccca tcgcgtcgcc aaccttgtag accagcacga aggccaggat
     1981 caggaaggcg ccgtgccgac ccagaaattc gcggaacggg ttgtagacgg tttccttgag
     2041 ccattgcccg gcgctcatgc ccgccgcttc ggcatagcga tcgatgaact tgcccggccc
     2101 ggcccagatc gctccggcga tcgcgggaat gatgcaaagc gcggtcaggc cgtaacccat
     2161 ggcccagccc agcccgagac cctcggtcga ggcgagatag accgtgcccg ccccggcaat
     2221 gagattgccg gtgcgatagc cgaactggtt ggtcgccgta ccgtgggcaa attcatcttc
     2281 ttccagaatt tcaatacgat aagcgtctat gacaatgtcc tgcgtggcgg aaaggaacgc
     2341 ggtgacaatc gcccagaata tgaagggccc catgtgcccg gtttgcggat cgctggcccc
     2401 cagttgccag atcgccacga acagcagcgc ctggacgaag aacagccagc cgcgccgctg
     2461 gccgaaaagc cgggtcagca cgggcagcgg caatttgtcg accagcggcg cccacaggaa
     2521 cttgatggta tagggcgtgc caagcgcgac cgcgaagccg atcgtggcgg tgtcgatccc
     2581 gaccttggcc agccagaagg tcatggtcgc taccagcagg gtca
//
LOCUS       DASUDV010000125         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_379191,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000125 DASUDV010000000
VERSION     DASUDV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379191"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /locus_tag="VFV30_02760"
     CDS             complement(<1..2552)
                     /locus_tag="VFV30_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4847041.1"
                     /translation="MSAQPARQPENSPSAPALREGGRAPGADGRTAPVDRAGLRSQAA
                     RTKRGSGGRRGSSRGDLPEQIDAGWAVMAPVFGAALVLIGLVSQSLPDGTALGLSLVA
                     MLGAALAPKAAELERRMLNRPSYRQALMVAVVGLPMLCWGLAVSYWTTEPGVSWDAVI
                     GLVVLVSVAASVLQHKRVSAIVVAQTALWSGLLIAGLSQGTLLAMLIGICGGLGITIR
                     QARIDQQEARRALERQRTQARAEEILHDFEQTGQGWFWETDRRGQLVYLSPPIAELLQ
                     LDLRKLIGRPLSDLFELEGQGQENERSLAFHLSARSAFTDLALRAATPGEERWWSVNG
                     RPLYDSFGNFLGFRGSGNDLTEKKRSEEQVSRLAHYDALTGLANRVQMGQTLEKILNA
                     PNHEHRACAVFLLDLDRFKQVNDTLGHPAGDALLTQVASRLEHAVEGKGRVGRLGGDE
                     FQVVLPGHPRRDELAHLANRIIRDLSEPYSIEGHRVMIGASVGIALSPDDGVSAEDII
                     RNADLALYAAKDGGRGRFHFYAADLHSDAEERRQLEQDLRDALTHGGLELHYQPVVHT
                     VTERITGFEALLRWHHPTRGPLSPAKFIPVAEDSGLIAPIGEWALRTACQHLAAWPES
                     VRVAVNVSPLQFANPALPAIITNALAAAQVDPGRLELEITESVFLSDDEGIEAMFSAL
                     KRIGVRLALDDFGTGYSALGYLKKAPFDKIKIDQSFVRGATITGSRNGAIISSIVSLA
                     ESLGMETTAEGVETLDELDLVRMLGCSHVQGYIYEKPLTHAAATERLRTGLTAIAQGP
                     RAARAVRHTMLRKVALENGGQHYHGTIRNISATGAMVEGLWNVPPGTIFTII"
     gene            2714..>4086
                     /locus_tag="VFV30_02765"
     CDS             2714..>4086
                     /locus_tag="VFV30_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4847042.1"
                     /translation="MAKGGTTLENEAAQSTMALGPLVGLAREDFVGAVALLLRETASD
                     PGRTVKHMQSFGDDVVKILTGKSDLAPDPKDKRFKDPAWQYNPFFRAGAQYYLAIQKG
                     MQGWLQDLELDELERDRANFISQIIIDGLAPTNTLVGNPTAQKRLIDSGGLSLIKGLK
                     NAYNDIVHNGGMVSQVDKTPFKLGENVATAKGAVVYRDAMFELVHYAPTTDEVYAIPQ
                     LTIPPQINKMYINDLSPEKSVVKWQTDNGIQCFVISWKNPTKEEGVWGMDDYIASCLK
                     AVDVVCEITGAPKVNISSACSGGQTGAILASKMAAAGDKRLGALTYMVTVLHPKQNDI
                     EAGSLMTENGLQLAKQRAAKAGIIKGDDLARGFAWLRPNDLIWNYVINNYLMGDDPPA
                     FDVLFWNADATNLSASLMGDFLHLFETLAFTRKGEVEMAGHKIDLSKVTSDLFILGGT
                     TDHITPWR"
BASE COUNT          717 a         1379 c         1352 g          638 t
ORIGIN      
        1 aagatgatcg tgaagatcgt gcccggcggc acgttccaca gcccttcgac catcgccccg
       61 gtggccgaga tgttgcggat cgtgccgtga tagtgctgcc cgccgttctc cagcgcgacc
      121 ttgcgcagca tggtgtggcg caccgcgcgg gccgcgcgcg ggccctgggc aatggcggtc
      181 aggccggtcc gcaggcgctc ggtcgcggcc gcgtgggtca gcggcttttc atagatatag
      241 ccctgcacgt ggctgcaccc cagcatgcgg accagatcga gctcgtcgag cgtctcgacc
      301 ccctcggcgg tggtttccat gcccagcgat tccgccaggc tgacgatcga ggagatgatc
      361 gccccgttgc ggctgccggt gatggtggca ccgcgcacaa agctctggtc gatcttgatc
      421 ttgtcgaacg gcgccttctt caaatagccg agcgccgaat agccggtgcc gaaatcgtcg
      481 agcgcaaggc gcaccccgat ccgcttcagc gccgagaaca tcgcctcgat cccctcgtcg
      541 tcgctcagga acacgctttc ggtgatttcc agctccagcc ggcccggatc gacctgggcc
      601 gcggccagcg cgttggtgat gatcgcgggc aaggccgggt tggcgaattg cagcggcgag
      661 acgttgaccg ccacccgcac gctttccggc caggccgcaa ggtgctggca ggcggtccgc
      721 agggcccatt cgccgatcgg ggcgatcagg ccggaatcct ccgccaccgg gatgaacttg
      781 gccggagaca gcggcccgcg ggtcgggtgg tgccagcgca gcagcgcctc gaacccggtg
      841 atccgttcgg tgacggtgtg aaccaccggc tggtaatgca gctccagccc gccgtgggtc
      901 agcgcatcgc gcaggtcctg ttccagctgg cgccgttcct cggcatcgct gtgcaggtcc
      961 gcggcataga aatggaaccg gccgcggccg ccgtccttgg cggcatagag cgccaggtcc
     1021 gcgttgcgga tgatgtcttc cgcgctcacc ccgtcgtccg gcgaaagcgc gatgccgacc
     1081 gaggcgccga tcattacccg gtggccttcg atcgaatagg gttcggacag gtcgcggatg
     1141 atgcgattgg cgaggtgcgc cagctcgtcg cggcgcgggt ggcccggcag cacgacctgg
     1201 aattcgtccc cgcccaggcg gccgacccgg cccttgccct ccaccgcgtg ttccagccgg
     1261 ctggcgacct gggtcagcag cgcgtccccg gccgggtggc ccagggtatc gttgacctgc
     1321 ttgaaccggt cgagatcgag caggaagacc gcgcaggctc ggtgttcgtg gttcggcgcg
     1381 ttgaggatct tctccagcgt ctggcccatc tgcacgcggt tggcgagccc ggtcagcgcg
     1441 tcatagtggg ccaggcggga aacctgttcc tcgctgcgtt tcttctcggt caggtcgttg
     1501 cccgatccgc ggaagcccag gaagttcccg aaactgtcat acagcgggcg gccgttgacc
     1561 gaccaccagc gctcctcgcc cggggtggcc gcgcgcagcg ccagatcggt aaaggccgac
     1621 cgcgcgctga ggtggaaggc cagcgagcgt tcgttctcct gcccctgccc ttccagttcg
     1681 aacaggtcgg acagcggccg gccgatcagc ttgcgcaggt ccaactgcaa cagctcggca
     1741 atcggcggag agagatagac cagctgcccg cgccggtcgg tttcccagaa ccaaccctgc
     1801 ccggtctgtt cgaaatcgtg caggatctcc tcggcacggg cctgggtgcg ctgccgctcc
     1861 agcgcgcggc gcgcctcctg ctggtcgatc cgggcctggc ggatggtgat ccccagaccc
     1921 ccgcaaatcc cgatcagcat ggccagcagc gtgccttgcg aaagacccgc aatcagcagg
     1981 cccgaccaca gcgcggtctg ggccacgacg atcgccgaaa cccgtttgtg ctgcagcacc
     2041 gaggcggcga ccgagaccag cacgaccagg ccgatgaccg cgtcccagct cacgccgggt
     2101 tcggttgtcc aataggatac ggccagaccc cagcagagca ttggcaggcc caccaccgcg
     2161 accatcagag cctggcggta ggacggccgg ttcagcatcc gccgttccag ctccgccgcc
     2221 ttgggggcaa gcgccgcgcc cagcatggcc accagcgaca ggcccagcgc cgtgccgtcc
     2281 ggcagcgact ggctgaccag cccgatcagg accagcgccg cgccgaacac cggggccatc
     2341 accgcccagc cggcatcgat ctgttcggga agatcgcccc gcgacgaacc gcggcgcccg
     2401 ccggagcccc gcttggtccg ggcggcctgt gagcgcagcc cggcccggtc gaccggcgcc
     2461 gtgcggccgt ctgcgccggg cgcacggccg ccctcgcgca aagcgggcgc ggaggggctg
     2521 ttttccggct gtctcgcagg ttgggcggac ataatgcgcc gcaatgccac gctagccttt
     2581 gaaaaaccgt taagttaacc atttggaccg gcggatttgc gcaaggccgc ccccggctgc
     2641 gcacggcgct tgaaatgcat tggacgcgcg ggcatcatcc cccgaacgac acaacagcga
     2701 acggggaaga cggatggcca agggcgggac cacgctggaa aacgaagcgg cccaatcgac
     2761 catggcgctg gggccgctgg ttggcctggc gcgtgaggat ttcgtcggcg cggtggcgct
     2821 gctgctgcgc gaaacggcca gcgatccggg ccggacggtc aagcacatgc agtccttcgg
     2881 ggacgacgtg gtcaagatcc tgaccggcaa gtcggatctg gcgcccgatc ccaaggacaa
     2941 gcggttcaag gacccggcct ggcagtacaa cccgttcttc cgcgccggcg cgcagtatta
     3001 cctggcgatc cagaaaggca tgcagggctg gctgcaagac ctggagctgg acgagctgga
     3061 gcgcgaccgg gccaatttca tctcgcaaat catcatcgac ggcctcgcgc cgaccaacac
     3121 cctggtcggc aacccgaccg cgcagaagcg cctgatcgac agcggcgggc tgagcctgat
     3181 caagggcctg aagaacgcct acaacgacat cgtccacaat ggcggcatgg tcagccaggt
     3241 ggacaagacc ccgttcaagc tgggcgagaa cgtcgccacc gccaagggcg cggtggtcta
     3301 tcgcgacgcg atgttcgaac tggtccacta tgcgcccacg acggacgagg tttacgcgat
     3361 cccgcagctg acaattccgc cgcaaatcaa caagatgtac atcaacgatc tgtcgccgga
     3421 aaagtcggtg gtgaagtggc agaccgacaa cggcatccag tgcttcgtga tctcctggaa
     3481 gaacccgacc aaggaggaag gcgtctgggg gatggacgat tacatcgcca gctgcctcaa
     3541 ggcggtcgac gtggtctgcg aaatcaccgg cgcgcccaag gtcaacattt ccagcgcctg
     3601 ttcgggcggg cagaccgggg cgatcctggc cagcaagatg gccgcggcgg gcgacaagcg
     3661 gctgggcgcg ctgacctata tggtcacggt cctgcacccc aagcagaacg atatcgaggc
     3721 cggctcgctg atgaccgaga acggcctgca gctggccaag cagcgcgccg ccaaggccgg
     3781 gatcatcaag ggcgatgatc tggcccgcgg ctttgcctgg ctgcgcccga acgacctgat
     3841 ctggaactat gtcatcaaca attacctgat gggcgatgat ccgccggcct tcgacgtgct
     3901 gttctggaat gccgacgcga ccaacctttc ggccagcctg atgggcgatt tcctgcacct
     3961 gttcgaaacg ctggctttca cccgcaaggg cgaggtcgag atggccgggc acaagatcga
     4021 cctgtccaag gtgaccagcg acctgttcat cctgggcggc accaccgatc acatcacccc
     4081 gtggcg
//
LOCUS       DASUDV010000126         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1837214,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000126 DASUDV010000000
VERSION     DASUDV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837214"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFV30_02770"
     CDS             complement(<1..263)
                     /locus_tag="VFV30_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4847043.1"
                     /translation="MKALRSHAVGGPETLTLDEVESPVPGPGQVRVKVKACSINFPDV
                     LIIRDMYQFKPPRPFAPGGEASGVVDAVGEGVTQWKVGDRVIAS"
     gene            complement(260..874)
                     /gene="leuD"
                     /locus_tag="VFV30_02775"
     CDS             complement(260..874)
                     /gene="leuD"
                     /locus_tag="VFV30_02775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX4847044.1"
                     /translation="MEAVRQVDGRAIPFGAKNVDTDVIIPAHWLKTITREGLGRGAFE
                     TLRQDPDNLFDSAEFSGAPILIAGDNFGCGSSREHAAWALLDLGITAVIAPSFSDIFS
                     GNAFKNGILTVVLPQDQVDRLLDVARTDPVHIDLENQVVTTPFQDRFEFEIDPFRKHC
                     LLNGLDEVGLTLARDTAISTYEHEARATMPWLGTVKSNLGVETA"
     gene            complement(874..1080)
                     /locus_tag="VFV30_02780"
     CDS             complement(874..1080)
                     /locus_tag="VFV30_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847045.1"
                     /translation="MSDTPEKPDSSDKPEKGDKPNWTGKAALAGAAIGSAALAAALLY
                     ASHRKERAEKAAKASTPKDVPETD"
     gene            1210..2421
                     /locus_tag="VFV30_02785"
     CDS             1210..2421
                     /locus_tag="VFV30_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4847046.1"
                     /translation="MTRRQALAGAAAVAAAAAPARAARSAPADFDVAVIGAGVFGAWT
                     AWHLKRRGLKVALLDAYAPGNARSSSGGESRVIRVSYGGDPLYSGMARESLSAWAKLS
                     SKQSLPLLHRTGVLWFSPAGDDYMARSIAWLKAHGIAHQAGDTAWLRARYPQITFKDG
                     ESGFLEEASGALIAGRGVQTVVADAGLTAQRVQAGPPERLSDGRLRIGGVTAHSAVYA
                     CGPWLPKLFPELLKDRIVATRQEVYHFGPAAGDGRFSPPALPVWADFNAGDIVYGFPG
                     LEGQGFKIAFDRHGAEVDPDSQDRRVSDEGVEQARAYLAKRFPGLAGAPFVHGRVCQY
                     ENSSNGDFLIDRLPGHDRVWLVGGGSGHGFKHGPAVGQRVAAHIADAALPVEPRFRLA
                     AKDKVQKRTVY"
     gene            complement(2415..>2708)
                     /locus_tag="VFV30_02790"
     CDS             complement(2415..>2708)
                     /locus_tag="VFV30_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847047.1"
                     /translation="LGQSYLGLLGLLALPLSGLYLDEFHQTTGYFYSYTDILGRRVAE
                     QRLGFWSDLPTDLKVILPLTAVLHVVGLVSAARCLLVRRELLPAGEAGRPGSQ"
BASE COUNT          402 a          879 c          980 g          447 t
ORIGIN      
        1 gaggcaatca cgcggtcgcc gaccttccac tgggtgacgc cttcgcccac cgcatcgacc
       61 acgcccgaag cctcgccgcc gggcgcgaag ggccggggcg gcttgaactg gtacatgtcg
      121 cggatgatca gcacgtccgg gaagttgatc gagcaggcct tgaccttgac ccggacctgg
      181 cccggaccgg ggaccgggct ttcgacttca tcgagggtca gcgtctcggg accgccgacg
      241 gcgtggctgc ggagggcttt catgcggttt ctactcccaa attgcttttg acggtgccga
      301 gccagggcat cgtggcccgg gcttcgtgtt cataagtgga aatggccgtg tcgcgggcca
      361 gggtcaggcc gacctcgtcc agcccgttga gcaggcagtg cttgcggaac ggatcgatct
      421 cgaactcgaa ccggtcctgg aacggcgtgg tgacgacctg gttttccaga tcgatgtgga
      481 ccgggtcggt cctggccacg tccagcagcc ggtcgacctg gtcctgcggc aggaccacgg
      541 tgaggatgcc gttcttgaag gcgttgccgg aaaagatgtc ggaaaaactg ggcgcgatca
      601 ccgcggtgat gcccagatcc agcagcgccc aggcggcgtg ctcgcggctt gatccgcagc
      661 cgaaattgtc acccgcgatc aggatcgggg ccccggaaaa ctcggcgctg tcgaacaggt
      721 tgtcgggatc ctggcgcagc gtttcgaacg cgccccggcc cagcccttcg cgggtgatcg
      781 tcttcagcca gtgggccgga atgatcacat cggtatcgac gttcttcgcg ccgaacggga
      841 tcgcccggcc atcgacctgc cgcacggcct ccatcaatcg gtttccggca cgtctttggg
      901 cgtggaggcc ttggcggcct tttcggcccg ttccttgcgg tgcgaggcat agagcagcgc
      961 ggcggccagc gcggccgaac cgatcgcggc cccggccagg gcggccttgc cggtccagtt
     1021 gggcttgtcg cccttctccg gcttgtcgct gctgtcgggc ttttcgggag tgtcgctcat
     1081 ccttccccta tgtccatcgc ggggcgcggg ttcaagggga gagggggaaa ttttcgcggg
     1141 gaggccttgg cctcgcggcg gggcggccat agtctgcggc gggatgcagg aggggacagc
     1201 gcgatggaca tgacccgcag acaggcactg gccggcgcgg ccgcggtggc ggctgccgca
     1261 gctccggccc gggcggcccg gtcggcaccg gccgatttcg atgtggcggt gatcggtgcc
     1321 ggggtgttcg gggcgtggac ggcctggcac ctcaagcgcc gggggctgaa ggtcgcgctg
     1381 ctcgatgcct atgccccggg caacgcccgc tcgtcctcgg gcggagagag ccgggtgatc
     1441 cgcgtctcct atggcggcga tccgctctat tccggcatgg cccgggaatc actgagcgcc
     1501 tgggcgaagc tgtcttccaa gcagagcctg ccgttgctcc accgcaccgg ggtgctgtgg
     1561 ttctcccccg ccggggacga ttacatggcg cgcagcattg cctggctgaa agcccacggc
     1621 attgcccacc aggccggcga caccgcctgg ctgcgggcgc gctatccgca gatcacgttc
     1681 aaggacggcg agagcgggtt cctggaggaa gccagcggcg cgctgatcgc ggggcgcggc
     1741 gtgcagaccg tggtggccga tgcgggcctc accgcgcagc gcgtgcaggc cgggccgccc
     1801 gaacggcttt ccgatggccg gctgcggatc ggcggcgtta ccgcccactc cgcggtctat
     1861 gcctgcgggc cgtggctgcc caagctcttt cccgaactgc tcaaggaccg gatcgtcgcc
     1921 acccggcaag aggtctatca ctttggcccg gctgcgggcg acgggcgctt ctcgccgccg
     1981 gccctgccgg tctgggccga tttcaacgcc ggggacatcg tctatggctt tccggggctg
     2041 gaagggcagg gcttcaagat cgcctttgac cgccacggcg ccgaagtcga tcccgacagc
     2101 caggaccggc gggtttcgga cgagggggtc gaacaggccc gggcctatct ggccaagcgg
     2161 ttcccgggcc tggccggcgc gcccttcgtt cacggccggg tctgccagta cgagaacagc
     2221 tccaacggcg atttcctgat cgaccggctg ccggggcacg accgggtctg gctggttggc
     2281 gggggatcgg gccacgggtt caagcacggc ccggccgtgg gtcagcgcgt ggcggcgcac
     2341 attgccgatg cggcgctgcc ggtggagccg cgcttccgcc tggccgcgaa ggacaaggtg
     2401 cagaaacgga cggtttactg actgccgggc cgtccggcct cgccggctgg cagcagctct
     2461 cggcgaacca gcaagcaccg cgcggccgag accaggccga cgacgtggag caccgcggtc
     2521 agcggcagga tgaccttgag gtccgttggc aagtcgctcc agaaccccag ccgctgttcg
     2581 gcaacgcggc ggcccaggat gtcagtgtaa gaatagaaat accccgtcgt ctggtggaat
     2641 tcgtcgaggt agagaccgga caggggcagg gcgagcaggc ccagcaggcc gagataggac
     2701 tggcccag
//
LOCUS       DASUDV010000127         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1594372,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000127 DASUDV010000000
VERSION     DASUDV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594372"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFV30_02795"
     CDS             <1..208
                     /locus_tag="VFV30_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEX4847048.1"
                     /translation="DSVKAASDVYSPVSGTITESNGALDGEPELVNSDPENAGWLFRL
                     TLSDPAELDGLMDKAAYDAFVAGL"
     gene            244..1602
                     /gene="gcvPA"
                     /locus_tag="VFV30_02800"
     CDS             244..1602
                     /gene="gcvPA"
                     /locus_tag="VFV30_02800"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX4847049.1"
                     /translation="MRYLPLTSADRAEMLGVIGAATVDDLFADVPAEARLTGPIAGLP
                     NHASEMAVERHMAALAGKNVSAGSVPFFLGAGAYRHHVPASVDHLIQRGEFLTAYTPY
                     QPEIAQGTLQVLFEFQTQVARLFGTDVANASMYDGSTACWEAVAMAGRVTKRSKAVLC
                     SGLHPHYVSTIRTMAKFTGDRIEFAAPRLEAEADTAGVIAAIDGETACVVVQYPDILG
                     RIPDLAAIAEAAHAAGALLVAVVTEPVALGLIEAPGHLGADIVVGEGQSLGVGLQFGG
                     PYLGLFGCREKFVRQMPGRLCGETVDAEGKRGYVLTLSTREQHIRREKATSNICTNAG
                     LCALAFSIHMTLLGGEGLSRLARVSHARAQQAAARLAKVPGVRVLNTAYVNEFTVVLP
                     TDAREAVRALADRRVLGGVSLGRLYPDAPDLACGLLVCTSELTTDADIEALGAALEGV
                     LA"
     gene            1599..3161
                     /gene="gcvPB"
                     /locus_tag="VFV30_02805"
     CDS             1599..3161
                     /gene="gcvPB"
                     /locus_tag="VFV30_02805"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX4847050.1"
                     /translation="MNAPNASGWRPEMGSAEAAPNQTASGDRALMLEEPLIFELGVPG
                     GTGVDIDAPSADPLPALGKHLRQAIPPLPGLSEPETVRHYTRLSRQNYAIDLGPFPLG
                     SCTMKHNPRLNEKVARLPGFADVHPLAPQQAVPGALEVINELAFWLIELTGMHGVAMS
                     PKAGAHGELCGVLCIKAALQARGEDRKVMLVPESAHGTNPATAAFAGFEIENIPAGAD
                     GRVDLAALEARLGPDVAGVMITNPNTCGLFERDMKRIGDAVHAAGGYVYCDGANFNAI
                     VGKVRPGDLGIDAMHINLHKTFSTPHGGGGPGSGPVVLSKALSPFGPLPYTVREADGS
                     VRLVEEEDAASEHPQGFGRMAAFHGQMGMFTRALAYILSHGADGLKQVAEDAVLNANY
                     VLRSLDGELDAPFGQAGPCMHEALFSDAGLAEGFTTLDIAKGLIDEGFHPMTVYFPLV
                     VHGAMLVEPTETESKAGLDRFIGSLRSLARRAKQGDPALKSAPHFAPRRRLDETQAAR
                     KPVLAWKGDQGE"
     gene            3164..3616
                     /locus_tag="VFV30_02810"
     CDS             3164..3616
                     /locus_tag="VFV30_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HEX4847051.1"
                     /translation="MWAGLFGFVNLLAMAAWAVLAFLPRRPLAHSAVLYLGTGLLCLI
                     YTVSMALLIGGAVAPGGQPDAGGLDLTSIEGVRRFFLSDAGLVIGWTHYLAFDLFTGL
                     WIARDADAKGFSRIAQLPFLALTFLAGPVGLLLWLVVRERRARAAGRG"
     gene            complement(3617..>3955)
                     /locus_tag="VFV30_02815"
     CDS             complement(3617..>3955)
                     /locus_tag="VFV30_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4847052.1"
                     /translation="RVSTNRYGQWVDVGFWIGQDGKVTDVEILRSSGDTGWVKPVLGN
                     ISRRVYAPLKAAGDSTPGFYMIERYTLTARVSDGPETGTRLRTREATPRIERLDLTEE
                     NYQAPPKEGG"
BASE COUNT          586 a         1321 c         1419 g          629 t
ORIGIN      
        1 cgacagcgtc aaggccgcca gcgacgtcta ttcgccggtc agcggcacga tcaccgaaag
       61 caacggagcg ctggatggcg agccggagct ggtcaattcc gatccggaaa acgccggctg
      121 gctgttccgc ctgaccctgt cagacccggc cgagctggac ggtctgatgg acaaggccgc
      181 ttacgacgcc ttcgtggccg gtctgtaatc cgtccctcaa gggagaggaa ttcgggagtt
      241 tccatgcgct acctgcccct gacctctgcc gatcgcgccg aaatgctggg cgtaatcgga
      301 gctgccacgg tcgatgacct gtttgccgat gtccccgccg aagcccggct gaccgggccg
      361 attgccggac tgccgaacca tgccagcgaa atggcggtgg agcggcacat ggctgccctg
      421 gccggcaaga acgtcagcgc cggatcggtg ccgttcttcc tgggggcggg ggcctatcgc
      481 caccacgtgc cggccagcgt cgatcacctg atccagcgcg gcgagttcct gaccgcctac
      541 acgccgtacc agcccgaaat cgcgcagggc acgctgcagg tgctgttcga gttccagacc
      601 caggtggcgc ggctgttcgg aacggacgtg gccaacgcct cgatgtacga tggctcgacc
      661 gcgtgctggg aagccgtggc gatggccggg cgggtgacca agcggagcaa ggcggtgctg
      721 tgttcggggc tgcacccgca ctatgtctcg accatccgga ccatggccaa gttcaccggc
      781 gaccggatcg aattcgccgc gccgcggctg gaggcagagg ccgacaccgc cggcgtgatc
      841 gccgcgatcg atggcgagac cgcctgcgtg gtggtgcagt accccgatat cctgggccgg
      901 atccccgatc tggcggcgat tgccgaggcc gcccatgcgg cgggcgcgtt gctggtcgcc
      961 gtggtgaccg aaccggtggc gctgggcctg atcgaggcgc cgggccatct cggcgcggat
     1021 atcgtggtgg gcgaggggca gtcgctcggc gtcgggctcc agttcggcgg gccctacctc
     1081 ggcctgttcg gctgccgcga gaagttcgtc cggcagatgc ccgggcggct ctgcggcgag
     1141 acggtcgatg ccgagggcaa gcgcggctat gtcctgacgc tgtcgacccg cgagcagcat
     1201 atccggcgtg agaaggcgac cagcaatatc tgcaccaatg cagggctttg cgcgctggcc
     1261 ttcagcattc acatgaccct gctgggcggg gaagggctga gccggctggc ccgggtcagc
     1321 cacgcccgcg cacagcaggc cgcggcgcgg ctggccaagg tgccgggggt gcgggtgctc
     1381 aacaccgcct atgtcaacga attcaccgtg gtcctgccga ccgacgcccg cgaagcggtg
     1441 cgggccctgg ccgaccgccg cgtgctgggc ggggtctcgt tgggccggct ctatcccgat
     1501 gcgcccgatc tggcctgcgg gctgctggtc tgcaccagcg agctgaccac cgacgcggat
     1561 atcgaagcgc tgggtgcagc gctggaagga gtgctggcat gaacgcgccc aacgcatccg
     1621 gctggcgccc cgaaatgggc agcgccgaag ccgccccgaa ccagaccgcc agcggagacc
     1681 gcgcgctgat gctggaagag ccgctgatct tcgaactggg cgtgccgggc ggcaccgggg
     1741 tcgatatcga tgcgccttcg gctgatccgc tgcccgcgct gggcaagcat ctgcgccagg
     1801 cgatcccgcc gctgccgggc ctgtccgaac cggaaacggt gcggcactat acccgcctgt
     1861 cgcgccagaa ctatgcgatc gatctggggc cgttcccgct gggatcgtgc accatgaagc
     1921 acaacccgcg gctgaacgag aaggtcgctc ggctgcccgg ctttgccgat gttcatccgc
     1981 tggcgccgca gcaggccgtg cccggcgcgc tggaagtgat caacgagctg gccttctggc
     2041 tgatcgagct gaccggcatg cacggcgtgg cgatgagccc caaggccggc gcccacggcg
     2101 agctgtgcgg ggtgctgtgc atcaaggcgg cgctgcaggc ccggggcgag gaccgcaagg
     2161 tcatgctggt gcccgaaagc gcccacggca ccaatcccgc caccgcggcc tttgccggtt
     2221 tcgagatcga gaacatcccg gcgggcgcgg atgggcgggt cgatctggcc gcgctggaag
     2281 cccggctggg tccggacgtg gccggggtga tgatcaccaa ccccaacacc tgcgggctgt
     2341 ttgagcgcga catgaagcgg atcggcgatg cggtccacgc cgcgggcggc tatgtctatt
     2401 gcgacggggc caatttcaac gcgatcgtcg gcaaggtccg cccgggcgac ctgggcatcg
     2461 atgcgatgca catcaacctg cacaagacct tttccacccc gcacggcggg ggcgggccgg
     2521 ggtccggtcc ggtggtcctg tccaaggcgc tcagcccgtt cggtccgctg ccctacaccg
     2581 tgcgcgaggc ggacggttcg gtgcggctgg tcgaggaaga ggacgccgcg agcgagcacc
     2641 cgcagggctt tggccggatg gccgcgttcc acgggcagat gggcatgttc acccgcgcgc
     2701 tggcctatat cctcagccac ggggccgacg gcctgaagca ggtggccgag gacgcggtgc
     2761 tcaacgccaa ctatgtcctg cgcagcctgg acggcgaact ggacgccccg ttcgggcagg
     2821 ccgggccctg catgcacgag gcgctgttca gcgatgccgg gctggccgaa ggcttcacca
     2881 cgctcgacat cgccaagggc ctgatcgacg agggcttcca cccgatgacg gtctatttcc
     2941 cgctggtggt ccacggcgcg atgctggtcg agccgaccga gaccgagagc aaggccgggc
     3001 tggaccgctt catcggatcg ctgcgcagcc tggcgcggcg ggccaagcag ggcgatccgg
     3061 cactgaaaag cgcgccgcac tttgccccgc gccgccggct ggatgaaacc caggccgcgc
     3121 gcaagccggt gctggcgtgg aagggcgatc agggagaata gcgatgtggg cagggctgtt
     3181 cgggttcgtc aacctgctgg ccatggccgc ctgggcggtg ctggccttcc tgccgcgccg
     3241 cccgctggcg cacagcgcgg tgctctatct cggcaccggg ctgctgtgcc tgatctacac
     3301 cgtgtccatg gcgctgctga tcggcggcgc agtggctccc ggcggccagc cggatgcggg
     3361 cgggctggac ctgacctcga tcgagggcgt gcggcgcttt ttcctgagcg atgccggcct
     3421 ggtgatcggc tggacgcact atctggcgtt tgacctgttc accgggctgt ggatcgcgcg
     3481 cgatgccgat gccaagggct tttcgcggat cgcgcagctg ccgttcctgg ccctgacctt
     3541 cctggccggg ccggtgggcc tgctgctgtg gctggttgtg cgcgaacgcc gcgcccgggc
     3601 cgccgggcgc gggtgatcag ccaccctcct tgggcggggc ctgatagttt tcttcggtca
     3661 ggtccagccg ctcgatccgc ggcgtggctt cgcgcgtgcg caggcgggtg ccggtttccg
     3721 gcccgtcgct gacccgcgcg gtcagggtgt agcgctcgat catgtagaag cccggggtgc
     3781 tgtcgcccgc ggccttgagc ggggcgtaga cccgccgcga aatgttgccc agcaccggct
     3841 tgacccaacc ggtgtcaccc gacgagcgca ggatctccac gtcggtcacc ttgccgtcct
     3901 ggccgatcca gaacccgaca tcgacccact ggccatagcg gttggtcgaa acgcg
//
LOCUS       DASUDV010000128         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_445526,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000128 DASUDV010000000
VERSION     DASUDV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445526"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..65)
                     /locus_tag="VFV30_02820"
     CDS             complement(<1..65)
                     /locus_tag="VFV30_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEX4847053.1"
                     /translation="MAKRILVVEDNDLNRKLFCDVL"
     gene            141..371
                     /locus_tag="VFV30_02825"
     CDS             141..371
                     /locus_tag="VFV30_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HEX4847054.1"
                     /translation="MLALGALGWVLGDGNRAARLLALTGLDADALRAGLGDPAVLGAV
                     LDFLAAHEPDLVAAAAALGIEPGELMAARERL"
     gene            376..990
                     /locus_tag="VFV30_02830"
     CDS             376..990
                     /locus_tag="VFV30_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4847055.1"
                     /translation="MARPLIISDCDEVLLHMIVPFGEWLEETQPVSFKLIGNDFSKAI
                     RDKQSGEPVEPMEIWRLLNLFFEGEMHRQSPIAGAVGAINTLKDHADVVILTNLADRH
                     NEARRHQLAAHGIDARVFTNQGPKGPALQAILEEYRPSRAVFIDDLAQHHGSAAEHVP
                     EIDRLHLCGEPTLAPHITCGFEAGHAHARIDTWEHALPWLLSRL"
     gene            1000..1461
                     /locus_tag="VFV30_02835"
     CDS             1000..1461
                     /locus_tag="VFV30_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4847056.1"
                     /translation="MSIEARLAELGITLPEPAAPVAAYVPVVIAGGLAHVSGQVSFVD
                     GVLVKGRLGEDVSLEQGVEAAQGCGLMILAQLKAALGSLDRVERVVKLGCFVNSSAQF
                     TDQPKVANGASELMVAVFGDAGKHARSAVGVPTLPLGVAVEVDAIVAVRGA"
     gene            1442..>2163
                     /locus_tag="VFV30_02840"
     CDS             1442..>2163
                     /locus_tag="VFV30_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4847057.1"
                     /translation="MLFAALDRWLAPAPDPARVSWLGEWRYAHRGLHGPAVPENSPSA
                     FAAAIEKGMGIECDIQRSLDGQAMVFHDTTLDRLTAQTGPLAERSAEALGKIVLTGSA
                     DTIPTLRELLRQIGGKVPLLIEIKSERSDGRRVRAICLAVRRELEGYPGPHAIMSFDP
                     RVVRWFADHSRLTLRGLVMTEEDDKALPGMIRRRLWLWHARPDFLAYDIRDLPSRFAA
                     AQRRRGLPVLTWTVRSAEHVERA"
BASE COUNT          351 a          696 c          742 g          374 t
ORIGIN      
        1 agcacatcgc agaacagctt gcgattgagg tcgttgtcct cgacaacaag gatgcgcttt
       61 gccatgagcg ctgccctaac ccctggcgga aaggctgact agtgctcagc gaacccaaat
      121 ccccggctca tgatgccgcc atgctggcgc tcggtgcgct cggctgggtg ctgggcgatg
      181 ggaaccgggc ggcgcggctg ctggccctga ccgggcttga tgccgatgcc ctgcgtgcag
      241 ggctgggcga tccggcggtg ctcggtgcgg tgctcgattt cctggccgcg cacgaacccg
      301 acctggtggc tgccgccgcc gcgctgggga tcgaaccggg cgaactcatg gcggcaagag
      361 agaggttgta ggacaatggc aaggccgctg atcatcagcg attgcgacga agtgctgctg
      421 cacatgatcg tacctttcgg cgaatggctg gaggaaaccc agccggtttc cttcaagctg
      481 atcggcaacg atttttccaa ggcgatccgc gacaagcaga gcggtgagcc ggtggaaccg
      541 atggagatct ggcggctgct taacctgttc ttcgaaggcg agatgcaccg ccagtcgccg
      601 attgccggcg cggtgggggc gatcaacacc ctgaaggacc acgccgacgt ggtgatcctc
      661 accaaccttg ccgatcgcca caacgaagcg cggcgccacc agctggccgc gcacgggatc
      721 gatgcccggg tcttcaccaa ccagggcccc aagggcccgg cgttgcaggc gatccttgag
      781 gaataccggc catccagggc ggtgttcatt gacgatctgg cgcagcatca cggatccgcg
      841 gccgagcatg tgcccgagat cgatcgtctt cacctgtgcg gcgaacccac gctggcgccg
      901 cacatcacct gtgggttcga ggcggggcat gcccatgccc ggatcgacac ctgggagcac
      961 gccttgccct ggctgctctc gcgactgtag gaagatatca tgagcatcga agccagactt
     1021 gccgaactgg gcatcaccct gcccgaacct gccgccccgg tcgccgccta tgtcccggtc
     1081 gtgatcgccg ggggacttgc ccatgtttcg gggcaggttt ccttcgtcga tggcgtgctg
     1141 gtcaagggcc ggctgggcga agacgtgtcg ctcgaacaag gggtcgaggc ggcgcagggt
     1201 tgcggcttga tgatcctggc ccagctcaag gccgcgctgg gttcgcttga ccgggtcgaa
     1261 cgcgtggtca agctggggtg cttcgtcaat tcgagcgctc agttcaccga tcagcccaag
     1321 gttgccaacg gcgcctcgga actgatggtc gcggtgtttg gcgatgccgg caaacatgcc
     1381 cgcagcgcgg tgggcgtgcc cacgctgccg ctgggggttg cggtggaagt ggacgcgatc
     1441 gttgctgttc gcggcgcttg accgctggct tgccccggcg cccgatccgg cccgggtaag
     1501 ctggctgggc gaatggcgct atgcccatcg cgggctgcat gggcccgccg tgcccgaaaa
     1561 ttcgccctcg gccttcgccg cagcgatcga aaagggcatg gggatcgaat gcgatatcca
     1621 gcgctcgctc gatggccagg cgatggtgtt ccacgacacg acgcttgacc ggctgaccgc
     1681 ccagactggc ccgctggccg agcgcagcgc cgaagcgctg ggcaagatcg tcctgaccgg
     1741 cagcgcggat accatcccca cattgcgcga gctcctgcgg cagatcggcg gcaaggtgcc
     1801 gctgctgatc gagatcaagt ccgaacgcag cgacgggcgc cgggtccgcg cgatctgcct
     1861 ggcggtccgg cgcgagcttg aaggctatcc cggcccgcac gcgatcatga gcttcgatcc
     1921 gcgggtggtg cgctggtttg ccgatcattc cagactgacc ctgcgcgggc tggtgatgac
     1981 cgaggaggat gacaaggcgc tgcccggcat gatccgccgc cggctttggc tgtggcatgc
     2041 ccggcccgat ttcctggcct atgacatccg cgatctgccc agccgctttg ccgccgcgca
     2101 gcgccgccgc gggctgccgg tgctgacctg gaccgtgcgc agcgccgagc acgtcgaacg
     2161 cgc
//
LOCUS       DASUDV010000129         6803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1329091,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000129 DASUDV010000000
VERSION     DASUDV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329091"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="VFV30_02845"
     CDS             complement(<1..2000)
                     /locus_tag="VFV30_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4847058.1"
                     /translation="MRISRRGVLIGAGVTGGLLAAWVLSPRSFAPPLPAGKDEFAFDA
                     WLKIGKDGVVSVAVPDVEMGQGITTLIPQIVAYELGADWRQIAVEPAPVSGAYGNAPL
                     AAHWAPLWIPLADGLANAPDSLLARRYAEGDAFVVTAEGTSLAAHETAARVAAASARA
                     VLAMAAADRWGVAWEECDAQNGFIVHDKQRASFAELVEEAVGYSPPSPAVARAAPPQE
                     RGAEFPAGAPLRYPRLDLPSKVDGSQVFAGDVRLPDMLHAAIRHAPIGDCALGSYDAE
                     RAKQVPGLVRLVEGKTWLAAVATDWWAAERALHAALPKFRHANLADSGKIAEWLEAAL
                     RRGKAARIAENGDPDEWLSEKFEHVARYEIAPALHGGLETATATARAEIGRVELWLAS
                     QAPQAARKAVAEAMGLPTDQVVIYPLPAGGSFDARLDAAHAVEAALIAREAGKPVQLT
                     WSRWQEHVAVPPRAPARAVLAARTAPDGSLTALKLRIAMPATVREFGARLLDGEDRRS
                     ALEQQNAHDPLAMQGMVPVYAVPHLVVEHVPAAIGLPTGRLRGNSNGIGAFLIETFID
                     ELASRSGREPLSYRMAMLGHDLKLANCLQRAATLAEWTGGRDGAGQGMACFRMVMTGR
                     EGRIAVIASARRDERGIRVDKLTAVADIGRIVNVDIARQQIEG"
     gene            complement(2021..2995)
                     /locus_tag="VFV30_02850"
     CDS             complement(2021..2995)
                     /locus_tag="VFV30_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX4847059.1"
                     /translation="MSNPDDAGQALQSALPPLAAARLQSLRLNQGVAIVVLDAGGLDA
                     VERERLEAAVKEVLSGQPGVSDVRVALTADKPRRRIVAVGSGKGGVGKSTVSTNLAVA
                     LARLGRKVGLVDADIYGPSQPRLLGNEGIKPQARKDKLIPVPSAWGVPVLSMGQLIEP
                     GKAIAWRGPMAGNALGQLVDADWGDAEILVVDLPPGTGDVQLTMLQRHKPAGAVIVST
                     PQDLALIDAKRAIQLFEVGQVPVIGMVENMAGYACPHCGEVSDPFGSGGAETAAAELG
                     MPFLGRIPLQLAIREASDAGTPPAAGEGPAAEAFAALARRVATWLDNQ"
     gene            3088..4167
                     /locus_tag="VFV30_02855"
     CDS             3088..4167
                     /locus_tag="VFV30_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEX4847060.1"
                     /translation="MSEPEGPKRRRKTKADSEATPAEATPPEAAAESPAPAPEPVPEQ
                     SGPPRNPWLPPAADEPAQRRSASIDDILRQRRLPGGGMPRPGSPGLAVWGVVGIVSAW
                     LLVTSVHVLAEGERGIVSTFGRYSSTIGPGLNLTLPWPIQQVTRRAVGAEVDTLLPDK
                     ESETLMLTRDGELIDVRVQVRWRVNDLRLFSTALPDGEAAVRRLTDSITRAAVAEMSF
                     DELRGGTRQAELQQRIKTRLQRVLTAWKSGVTVVTVAVTDTKPPARLAETFKTLAKEN
                     EAARKNHEEAEVYAQQVRVTANDEAAAFDRAYELYRVAPDAVREQMYYETVEKVLRNN
                     PVSFGESGTKPAAAPAANAKAEGGR"
     gene            4170..5006
                     /locus_tag="VFV30_02860"
     CDS             4170..5006
                     /locus_tag="VFV30_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4847061.1"
                     /translation="MVETLRRHRHSLFGLAILALLGLSSFVIVPDDQQAVVTRMGEPD
                     RVVNRFRPDGPSGAGIVARVPLMEQVVWLPRGLTTFSHQTKRVRSADEQWLLVDTDVT
                     YRIIDPVKLVNTLGSAGKIDGQLGALLPPLLDEALGKQTAGTIARPGSGGATAALLRG
                     LDQQTRKYGIQVIDLRIARLALDEKGRDEAFARMKERQEAEIMAVRQRKYAEAAEITQ
                     AALLEAAARSQQSAARDPEFYAFFQAMRSYERVYGDPNRKNSATFVLPPDSGYLKHFG
                     GK"
     gene            5089..6603
                     /locus_tag="VFV30_02865"
     CDS             5089..6603
                     /locus_tag="VFV30_02865"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX4847062.1"
                     /translation="MKYAYGVTSALLMGGAALSLVSGLPAGAQVAQNEQGPGSSLVPR
                     PGAPVSFADLTQQLQPAVVNIAVRQKVPVKNPLAGTAFGNLVPGGGGKTTVAEAVGSG
                     FIISADGYIVTNNHVISLDREGQADEVTVKLADGRQFKARIVGRDADSDLAVLKIDAP
                     APLPFVKFGDSGKARAGDWIIAIGNPFGLGGSVTSGIISSVNRNTGTGAYDHYIQTDA
                     SINSGNSGGPMFDMAGNVIGINNWIVAPPGGNGGNIGIGFAIPSDTAKPIVERLKSGQ
                     AIERGYLGIQIQPISDEIADSLGLPHDQGELVRGVLPGMPAAKAGIQVGDIVLKVNGT
                     PVNPDRTLSGIVSNVAPGTRIPVEVLRDGKRLTLYATVAKRPTQQELARSLGPTEKVD
                     PFANPQQQGEGLAESALGLKVLNMEPEIAQQLGVPLSQKGVVIAGVEGGSDAATRGLQ
                     RGDIVVSANMKGVASVGELEGAIRAAKTAGRPSVTLQILRPGQPGLVYIAIRLS"
BASE COUNT         1058 a         2441 c         2325 g          979 t
ORIGIN      
        1 ccctcgatct gctggcgggc aatgtcgacg ttcacgatcc ggccgatgtc ggccactgca
       61 gtcagcttgt cgacgcggat cccgcgctcg tcgcgccggg cgctggcgat caccgcgatc
      121 cggccctcgc ggccggtcat caccatgcgg aaacaggcca tcccctgccc ggccccgtcg
      181 cgcccgccgg tccattcggc aagggtcgcg gcgcgctgca ggcaattggc cagcttcaga
      241 tcgtgcccca gcatggccat ccggtaggac agcggctcgc ggcccgaacg gctggcgagt
      301 tcgtcgatga aagtctcgat caggaaggcc ccgatcccgt tcgaattgcc gcgcagccgc
      361 ccggtcggca ggccgatcgc ggcgggcacg tgttcgacca ccaggtgcgg cacggcatag
      421 accgggacca tgccctgcat cgccagcgga tcatgggcgt tctgttgctc aagcgcgctg
      481 cggcgatcct cgccatccag cagccgggcg ccgaattcgc gcaccgtcgc gggcatggcg
      541 atccgcagct tcagcgcggt cagcgatccg tccggcgcgg tgcgcgcggc cagcacggcg
      601 cgggccgggg cgcgcggcgg caccgcgaca tgctcttgcc agcgcgacca ggtcagttgc
      661 accggcttgc cggcctcgcg cgcgatcagt gccgcctcga ccgcgtgggc cgcatcgagc
      721 cgcgcatcga aactgccgcc ggcggggagc ggatagatca ccacctgatc ggtcggcagg
      781 cccatcgcct cggccaccgc cttgcgcgcg gcctgcgggg cctggctggc cagccacagc
      841 tcgacccggc cgatctcggc ccgggcggtc gccgtggcgg tttccaggcc gccgtggagc
      901 gccggggcga tttcgtagcg ggcgacgtgt tcgaactttt ccgacagcca ttcatcggga
      961 tcgccgtttt cggcaatccg ggcggccttg ccgcggcgca gcgccgcttc cagccattcg
     1021 gcgatcttgc cgctgtcggc caggttggcg tggcgaaact tgggcagcgc ggcgtggagc
     1081 gcgcgctcgg ccgcccacca gtcggtcgcc acggcggcca gccaggtctt cccttcgacc
     1141 agccggacca gccccggcac ctgcttggcc cgttcggcat cgtagctgcc cagcgcgcaa
     1201 tccccgatcg gggcgtggcg gatcgcggcg tggagcatgt cgggcaggcg gacgtcgccg
     1261 gcaaagacct ggctgccatc gaccttgctg ggcagatcga gccgcggata gcgcagcggc
     1321 gcgccggccg gaaattcggc cccgcgttcc tgcggcggcg cggcgcgggc cacggccgga
     1381 ctgggcgggc tgtaaccgac cgcctcttcc accagctcgg caaacgaggc gcgctgcttg
     1441 tcgtggacga tgaacccgtt ctgggcgtcg cattcctccc aggccacgcc ccagcggtcg
     1501 gccgcggcca tggccagcac ggcccgcgcg ctggccgcgg ccacccgggc ggcggtttcg
     1561 tgcgcggcca aggaggtgcc ctcggccgtc acgacaaagg catcgccttc ggcatagcgg
     1621 cgggcgagca ggctgtccgg cgcgttggcc aggccatcgg ccagcgggat ccacagcggc
     1681 gcccagtgcg cggccagcgg ggcattgcca taggcgccgc tgaccggggc cggctcgacc
     1741 gcgatctgcc gccagtccgc acccagttca taggcgacga tctgcgggat cagggtggtg
     1801 atgccctgcc ccatctcaac atcgggcacg gccacgctga ccaccccgtc cttgccgatc
     1861 ttcagccagg cgtcgaaggc gaactcgtcc ttgccggccg gcagcggcgg cgcaaagctg
     1921 cgcgggctga gcacccaggc cgccagcagc ccgccggtta cccctgcccc gatcagcacg
     1981 ccgcgccgcg aaatcctcat tgggcttgtc tagccgatgg ttactggttg tcgagccatg
     2041 tcgccacgcg gcgcgccagg gcggcaaagg cttcggcggc gggcccttcg ccggcggcag
     2101 gcggcgtgcc ggcatcgctg gcctcgcgga tcgccagttg cagcgggatc cggcccagga
     2161 acggcatccc cagctcggcc gcggccgtct cggccccgcc gctgccaaag ggatcgctga
     2221 cctcgccgca atgcgggcag gcatagccgg ccatgttctc aaccatgccg atcaccggca
     2281 cctggccgac ctcgaacagc tggatcgcgc gcttggcgtc gatcagcgcc aggtcctgcg
     2341 gggtggacac gatcaccgcc ccggccggct tgtgccgctg cagcatggtc agctgcacgt
     2401 cgccggtgcc cggcggcagg tccaccacca ggatttcggc atcgccccaa tcggcatcga
     2461 ccagctggcc cagcgcgttg ccggccatcg gcccgcgcca ggcgatcgcc ttgccgggct
     2521 cgatcagctg gcccatcgac agcaccggca cgccccatgc gctcggcacc gggatcagct
     2581 tgtccttgcg ggcctggggc ttgatcccct cgttccccag caggcgcggc tgcgaggggc
     2641 cgtagatatc ggcatcgacc aggccaacct tgcgccccag ccgcgccagc gcgaccgcca
     2701 ggttggtgga gacagtcgac ttgccgaccc cgcccttgcc cgagccgacc gccacgatcc
     2761 ggcggcgcgg cttgtccgcg gtcagcgcga cgcgcacgtc gctgaccccg ggctggccgg
     2821 acaggacctc tttcaccgcg gcttccagcc gctcgcgctc caccgcgtcg agcccgccgg
     2881 catcgagcac gacgatcgct accccctggt tcagccgcag cgattgcagc cgcgcggcag
     2941 cgaggggcgg gagggcggat tgcagggcct ggccggcgtc atcgggattg ctcatggccc
     3001 cgcgtggaag cgccttcgcc ccggactggc aaggaaaatc cgccgccacc cctgtttttt
     3061 gcccaaccgc cccctatagt aacctccatg agcgaacccg aaggcccgaa gcggcgcagg
     3121 aagaccaaag ccgacagcga agcaacgcct gccgaggcta cccctccgga agccgcggcg
     3181 gaatcgcctg cgccggcgcc ggagcccgta ccggagcaat ccggcccgcc gcgcaacccc
     3241 tggctgcccc ccgcggcgga cgagccggcc cagcgccgct cggccagcat cgacgacatc
     3301 ctgcgccagc gccgcctgcc cggcggcggg atgccgcgcc ccggttcgcc ggggctggcg
     3361 gtgtggggcg tggtcgggat cgtctcggcc tggctgctgg tcacctcggt ccatgtcctg
     3421 gccgaaggtg agcgcgggat cgtctccacc ttcgggcgct attccagtac gatcgggccg
     3481 ggcctcaacc tgacgctgcc ctggccgatc cagcaggtca cccggcgggc ggttggcgca
     3541 gaggtggaca ccctgctgcc cgacaaggaa tccgaaacgc tgatgctgac ccgcgacggc
     3601 gagctgatcg acgtgcgggt ccaggtgcgc tggcgggtga acgacctgcg cctgttcagc
     3661 accgccctgc ccgatggcga agcggcggtg cggcggctga cggattcgat cacccgcgcc
     3721 gcggtggcgg aaatgagctt tgacgaattg cgcggcggaa cccgtcaggc cgaactgcag
     3781 cagcggatca agacccggct gcagcgggtg ctgaccgcgt ggaagagcgg ggtgaccgtg
     3841 gtcaccgtcg ccgtgaccga caccaagccc ccggcccggc tggccgaaac cttcaagacc
     3901 ctggccaagg agaacgaggc cgcgcgcaag aaccacgaag aagccgaggt ctatgcccag
     3961 caggtccgcg tcaccgccaa cgacgaggcc gcggcctttg accgggccta tgagctctac
     4021 cgcgtcgcgc ccgatgctgt gcgcgagcag atgtactacg agacagtcga aaaagtgctg
     4081 cgcaacaacc cggtctcctt tggagaaagc ggcacgaagc cggcagcggc gcccgctgca
     4141 aatgccaaag ccgaaggagg gcgctgacca tggttgagac cttgcgccgc caccgccatt
     4201 cgctgttcgg ccttgccatc ctggccttgc tgggcctgtc ctcgttcgtg atcgttccgg
     4261 atgaccagca ggccgttgtc acccgaatgg gcgagccgga ccgggtggtg aaccggttcc
     4321 gccccgatgg ccccagcggg gccgggatcg tggcccgggt acccttgatg gaacaggtcg
     4381 tctggctgcc ccgcggcctc accactttca gccaccagac aaagcgcgtg cgcagcgcgg
     4441 acgagcagtg gctgctggtc gataccgacg tgacctaccg gatcatcgat ccggtcaagc
     4501 tggtgaacac gctgggcagc gccggcaaga tcgacggcca gctgggcgcg ctgctcccgc
     4561 cgctgctgga cgaggcgctg ggcaagcaga ccgccggcac catcgcccgg ccgggatcgg
     4621 gcggcgccac cgccgcgctg ctgcgcgggc tggaccagca gacccgcaag tacgggatcc
     4681 aggtgatcga cctccggatc gcccggctcg cgctcgacga gaagggccgc gacgaggcct
     4741 ttgcccggat gaaggaacgg caagaggccg agatcatggc ggtgcgccag cgcaaatatg
     4801 ccgaagcggc cgagatcacc caggccgccc tgctggaagc ggcggcccgt tcgcagcaaa
     4861 gcgcggcgcg cgatcctgaa ttctacgcct tcttccaggc gatgcgcagc tacgaacggg
     4921 tctacggcga ccccaatcgc aagaattcgg caactttcgt gctgccgccc gattccggct
     4981 atctgaagca ctttggtggc aaatgagcca ttcaatcccc attaagggcc tctgcgatca
     5041 aatccgccgt ccggggcgga tgaacatgac aacgaaggat caggcaacgt gaaatacgct
     5101 tacggtgtga cttccgcgct gctgatgggc ggcgccgcgc tttcgctggt cagcggcctt
     5161 ccggccggtg cccaggtggc ccagaacgag cagggccccg gcagttcgct ggtgccccgc
     5221 cccggcgcgc cggtcagctt tgccgacctg acccagcaat tgcagcccgc ggtggtcaac
     5281 atcgcggtgc gccagaaagt gccggtcaag aacccgctcg ccggaaccgc ctttggcaac
     5341 ctggtgccgg gcggcggcgg caagaccacg gtggccgaag cggtcggttc gggcttcatc
     5401 atttcggccg atggctacat cgtcaccaac aaccacgtga tcagcctcga ccgcgaaggc
     5461 caggccgacg aagtgacggt caagctggcc gatgggcgcc agttcaaggc ccggatcgtc
     5521 gggcgcgatg cggattcgga ccttgcggtc ctcaagatcg acgcgccggc gccgctgccc
     5581 ttcgtcaagt tcggcgattc cggaaaggcc cgcgccggcg actggatcat cgcgatcggc
     5641 aacccgttcg ggctgggcgg atcggtcaca tcggggatca tatcctcggt caaccgcaac
     5701 accggcaccg gggcctacga tcactacatc cagaccgacg cctcgatcaa ttcgggcaat
     5761 tccggcgggc cgatgttcga catggccggc aacgtgatcg gcatcaacaa ctggatcgtg
     5821 gcgcctccgg gcggcaacgg cggcaacatc gggatcggct ttgcgatccc gtccgacacc
     5881 gccaagccga tcgtcgagcg gttgaagagc ggccaggcga tcgagcgcgg ctatctgggc
     5941 atccagatcc agccgatttc cgacgagatc gcggattcgc tgggcctgcc gcacgaccag
     6001 ggcgaactgg tccgcggcgt gctgcccgga atgccggcgg ccaaggccgg gatccaggtc
     6061 ggcgatatcg ttctcaaggt gaacggcacg ccggttaatc ccgaccgcac gctgtcgggc
     6121 atcgtctcca acgtcgcgcc cggcacccgc atcccggttg aagtgctgcg cgatggcaag
     6181 cggctgacgc tctatgccac ggtcgccaag cgcccgaccc agcaggaact ggcccgcagc
     6241 ctgggtccga ccgaaaaggt cgatcccttc gccaacccgc agcagcaggg cgaaggcctg
     6301 gcggaaagcg cgctggggct gaaagtgctc aacatggagc ccgagatcgc ccagcagctg
     6361 ggcgtgccgc tgagccagaa gggcgtggtc atcgccgggg tcgaaggcgg atcggatgcc
     6421 gcgacccgcg gcctgcagcg cggcgacatc gtcgtttcgg ccaacatgaa gggtgtcgcc
     6481 tcggtcggcg aactcgaagg cgcgatccgc gccgcgaaga cggccggccg gcccagcgtg
     6541 accctgcaga tcctgcgtcc cggccagccc ggcctggtct acatcgccat ccggctgagc
     6601 tgaacctctg gcctgagcgg agccccggcg gtccggggcc cgctattggc ctagtcgccc
     6661 ggcggggcgg cggcggccgg cttggtgcct ccgcccgtcg cctcgttgag gaaatcctcg
     6721 ttcgcggccg gcggcggcgt caccggcacc agcccgccgt tgtttgacgg caccggatcc
     6781 tcgcgcgggc gttccggcgc gat
//
LOCUS       DASUDV010000130         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1351616,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000130 DASUDV010000000
VERSION     DASUDV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351616"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..848
                     /locus_tag="VFV30_02870"
     CDS             21..848
                     /locus_tag="VFV30_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847063.1"
                     /translation="MTFAGQTAWITGASSGIGAALARGLAKQGAHLVLSGRNVEALQE
                     VAKDCGETLILPFETTDIAAIAPAVAQAWDWAASRSGGIDLLVNNAGISQRSLAVDTA
                     YAVYEKIVGVDLLAPIALTQENLPRMVARGSGRIAMISSIAGKVGVPMRTAYCAAKFG
                     IAGYADALRAEVGYLGLQVHNIYPGSVATDVSRNALTADGSKRGVSDQVIDNGIPPDV
                     AVAQMIDEMLAGQREIIVAEGAEKAMGEARRTPDALLDQIGAFMAAGYAQRMNEGAK"
     gene            851..1753
                     /locus_tag="VFV30_02875"
     CDS             851..1753
                     /locus_tag="VFV30_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847064.1"
                     /translation="MQRVTLANGIELDVQDEGPRDAPVLIFLHGFPDSHRTWRHQIAH
                     LKDRFRCIAPDQRGYRGSSKPKGVESYTPDKLIGDVFLLADALGVGTFTIVGHDWGGA
                     IAWGVAIMGQMNGRVTRAVVANAPHPTIFPKLLYTNRDQRQASQYFRIFRDPASDALV
                     EQFGLGGVLLKAFGETPENPKMEPEERAALLADWENREAAIAMLNWYRASPMEVLDMD
                     APYELPAGWQPVPLPRLTIPTLVVWALDDIALPPANLDGMDELIADLTVERIPGCGHF
                     VQWEAPTQVNAALDAFLARTADAA"
     gene            1743..>2488
                     /locus_tag="VFV30_02880"
     CDS             1743..>2488
                     /locus_tag="VFV30_02880"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX4847065.1"
                     /translation="MPPEAPRGGIGAAFARAEKTARRSMLRNHGGGHAQVSFLELFFD
                     LVFVFAITQLSHFLKDNLTWLGFAQSLVLFLAVWWAWMYTTWATNWANPERFEVRVTL
                     LVLMALSLVMAVAMPYAFGKYAWLFALCYCAMQIGRTVFMAVILRGANPGNSRNMVRI
                     ALWFCASALLWGAGALRGEEERLVWWTLAVGLEYLGPIALFRTPGLGHSQPSDWDISG
                     SHMAERCGLFIIIALGEGIIITGASFAKEE"
BASE COUNT          400 a          832 c          853 g          403 t
ORIGIN      
        1 aacgacgcag ggagagacca atgacttttg ccggacaaac cgcctggatc acgggtgcct
       61 cttcggggat cggcgccgcg ctggcccggg gcctggccaa acagggcgcg cacctggtcc
      121 tgtccggccg aaacgtcgag gcgttgcagg aggttgcgaa ggactgcggc gaaacgctga
      181 tcctgccgtt cgaaaccacc gacatcgccg cgattgcgcc ggcggtggcc caggcgtggg
      241 actgggccgc ctcgcgctcg ggcgggatcg acctcttggt caacaacgcc ggcatttcgc
      301 agcgcagcct cgcggtcgac accgcctatg cggtttatga gaagatcgtc ggcgtggatc
      361 tcctcgcccc gatcgcgctg acgcaggaga acctgccgcg gatggtcgcg cgcggcagcg
      421 ggcggatcgc gatgatcagc tcgatcgccg gcaaggtcgg cgtgccgatg cgcaccgcct
      481 attgcgccgc caagttcggc atcgccggct atgccgacgc gctgcgagcc gaggtcggct
      541 atctgggcct gcaggtccac aacatctacc ccggctcggt cgccaccgat gtctcgcgca
      601 acgcgctgac cgcggacggc agcaagcgcg gcgtatccga tcaggtgatc gacaacggca
      661 ttccgcccga tgtggccgtg gcgcagatga tcgacgagat gctggccggc cagcgcgaaa
      721 tcatcgtggc cgaaggggcc gaaaaggcca tgggcgaagc ccgccgcacg cccgatgccc
      781 tgcttgacca gatcggcgcc ttcatggccg ccggctatgc ccagcggatg aacgaggggg
      841 cgaagtgacc atgcagcgcg taaccctggc caacgggatc gagctggacg tccaggacga
      901 aggcccgcgc gatgcgccgg tgctgatctt cctgcacggc tttccggaca gccaccgcac
      961 ctggcggcac cagatcgccc acctgaaaga ccggttccgc tgcatcgcgc ccgaccagcg
     1021 cggctaccgc ggctcgtcca agcccaaggg ggtggagagc tacacgcccg acaagctgat
     1081 cggcgacgtc ttcctgctgg ccgatgcgct gggcgtgggc acgttcacca tcgttggcca
     1141 cgattggggc ggcgcgattg cctggggcgt ggcgatcatg ggccagatga acggccgggt
     1201 gacccgcgcg gtggtggcca acgccccgca tcccacgatc ttccccaagc tgctctacac
     1261 caaccgcgat cagcgccagg cgagccagta tttccggatc ttccgcgatc cggccagcga
     1321 cgcgctggtc gagcagttcg gccttggcgg cgtgctgctg aaggcctttg gcgagactcc
     1381 ggaaaacccg aagatggaac cggaagagcg cgccgcgctg ctggccgact gggaaaaccg
     1441 cgaagccgcg atcgccatgc tcaactggta ccgcgccagc ccgatggaag tgctggacat
     1501 ggacgcgccc tatgaactgc cggccggctg gcagccggtg cccctgcccc gcctgaccat
     1561 cccgacgctg gtggtctggg cgctggacga catcgccctg cccccggcca acctggacgg
     1621 gatggacgaa ctgatcgccg acctgaccgt cgaacgcatc ccgggctgcg gccatttcgt
     1681 gcagtgggaa gccccgacgc aggtcaacgc cgcgctggat gccttcctgg cccggaccgc
     1741 cgatgccgcc tgaggcgccg cgcggaggga tcggcgcggc ctttgcccgg gccgaaaaga
     1801 ccgcgcggcg atccatgctg cgcaaccacg gcggcgggca cgcgcaggtc agctttctgg
     1861 agctgttctt cgacctggtg ttcgttttcg cgatcaccca gctctcgcac ttcctgaagg
     1921 acaacctcac ctggctgggc tttgcccagt cgctggtgct gttcctggcg gtgtggtggg
     1981 cctggatgta caccacctgg gccaccaact gggccaaccc ggaacggttc gaagtgcggg
     2041 tgacgctgct ggtgctgatg gcgctcagcc tggtgatggc cgtggccatg ccctatgcct
     2101 ttggcaagta cgcctggctg ttcgcgctgt gttactgcgc gatgcagatc gggcgaacgg
     2161 tgttcatggc cgtgatcctg cgcggggcga acccgggcaa ttcccgcaac atggtgcgga
     2221 tcgcgctgtg gttctgcgcc tccgcgctgc tctggggcgc gggcgcgctg cgcggggagg
     2281 aggagcggct ggtctggtgg accctggccg tggggctgga atacctcggt ccgatcgcgt
     2341 tgttccgcac tccgggcctg ggccattcgc agcccagcga ctgggacatt tccggcagcc
     2401 acatggccga acgctgcggc ctgttcatca tcatcgcgct gggcgaaggg atcatcatca
     2461 ccggggcctc gttcgccaag gaagagat
//
LOCUS       DASUDV010000131         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_534358,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000131 DASUDV010000000
VERSION     DASUDV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534358"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFV30_02885"
     CDS             <1..491
                     /locus_tag="VFV30_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEX4847066.1"
                     /translation="GFVPCTPLGCLMLLKDRLGSLSGLDAVVIGRSNIVGKPMAQLLL
                     AESCTVTIAHSRTKDLAEVVRRADIVVAAVGRPEMVKADWIKPGATVIDVGINRLPPA
                     DGESKGRLVGDVDYAGAAAVAGAITPVPGGVGPMTIAVLLRNALVAAHRNAGVALADG
                     AL"
     gene            536..1240
                     /locus_tag="VFV30_02890"
     CDS             536..1240
                     /locus_tag="VFV30_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847067.1"
                     /translation="MKRMILWGLAFSLAAAPALSRDRRDGDGHGNANPSAVVAAEVAF
                     AQLAQEKGQWTAFRTTATKDAVMFVPQMAYAQAWLKDRPDPAKAVTWQPHQVWMSCDG
                     TLAVTRGAWQGPNSTSGYFTTVWQRQKDGKYKWVLDQGEELPMPLDPPDMIVGKVADC
                     PPGYRPGRGPRPKDFKGNLPPLDPARRSGQSLDGTLGWDVTVSPEGARRFTVTMQLEG
                     ARATVQDLQVEAPAKK"
     gene            1240..1863
                     /locus_tag="VFV30_02895"
     CDS             1240..1863
                     /locus_tag="VFV30_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX4847068.1"
                     /translation="MWELFLSAFVTLFVVIDPPGCAPIYAGLVKGANNRLATAMAVRA
                     TFIAAIILLVFALFGEDLLGALHIELNSFRIAGGIMLFVIAMDMVFEKRTQRREERAE
                     KVREEHVEDVSVFPMAMPMIAGPGSIATIMLLTARAQGTEQTLVVLAAMGAVLLLTLL
                     ALVAAGPLMKLLGARVEAVITRLLGVLLAALAAQYVIDGIKAVLVAT"
     gene            1878..2411
                     /locus_tag="VFV30_02900"
     CDS             1878..2411
                     /locus_tag="VFV30_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847069.1"
                     /translation="MTVDQAQAALRQAFAHGGPGAVVSALVWFAAAAAFVRTDHATAF
                     AVLFFGGMLIFPVSSLIGKLAFGRSASAKGNPLGLLALESTIAMIGCLIAAWLLLKDR
                     PDWVFPVAAIAVGTHYLPFKTAYGDRTYWLLGAAITVIGCAGIFVLPGQTLAVILAVA
                     VTELVFGLALTRAGTRV"
     gene            complement(2408..2845)
                     /locus_tag="VFV30_02905"
     CDS             complement(2408..2845)
                     /locus_tag="VFV30_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847070.1"
                     /translation="MTPLSGDTPMPAVRFAPLALAAAALAFAVPGAVHAAGKGNANGH
                     GSHAGHAGHAPAAKKPPKAARAKPCPPGLAKKNTGCLPPGQWRKGDRLPDAWAAQFIA
                     YAALPDFYRSRYPAQDGRRYLYRDGRVFVVDAITRTIVDVILR"
     gene            complement(2896..3501)
                     /locus_tag="VFV30_02910"
     CDS             complement(2896..3501)
                     /locus_tag="VFV30_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX4847071.1"
                     /translation="MRVSIFPLPGAILFPGLQLPLHIFEPRYRALVKDALARDRRIGM
                     IQPQRAEEGAPLYQIGCLGKIGEVEALDDGRFNLLLEGEARFRLVRELDAATPFRQIE
                     AELLDEEDYPALSPVERAGFEREARRFSDSQGYSVDWEAVTRLDDESLINGTSQIVPF
                     DIASKQALLEAPSLATRCELLVQLMQFFGRRDSDDPQVTLQ"
BASE COUNT          525 a         1178 c         1228 g          576 t
ORIGIN      
        1 cgggcttcgt gccctgcacg ccgctgggct gcctgatgct gctgaaggac cggctgggtt
       61 cgctgtcggg cctcgatgcg gtggtgatcg ggcgctccaa catcgtcggc aagccgatgg
      121 cccagctgct gctggcggaa agctgcaccg tgacgatcgc ccacagccgg accaaggatc
      181 tggccgaagt ggtgcgccgc gccgatatcg tggtggctgc ggtcggccgg ccggaaatgg
      241 tcaaggcgga ctggatcaag cccggcgcga ccgtgatcga tgtcggcatc aaccgcctgc
      301 cacccgccga tggcgagagc aaggggcggc tggttggcga tgtcgactat gccggagcgg
      361 ccgcagtggc cggagcgatc accccggttc cgggcggggt cgggccgatg accatcgccg
      421 tcctgctgcg caacgcgctg gtcgcggcgc accgcaacgc cggtgtggcc ctggccgacg
      481 gggcgctgtg acgttcgact cgccacgctt cccgcccggc gctaaacccg gcgtcatgaa
      541 acgcatgatt ctctgggggc ttgcgttctc gcttgccgct gcacccgccc tgtcgcgcga
      601 tcgccgggat ggcgatggcc acggcaacgc caatcccagc gccgttgtcg cggccgaagt
      661 ggcctttgcc cagcttgccc aggaaaaggg ccagtggacc gcgttccgca ccaccgcgac
      721 caaggacgcg gtgatgttcg tgccgcagat ggcctatgcc caggcctggc tgaaggaccg
      781 ccccgatccc gccaaggcgg tgacctggca gccgcaccag gtgtggatga gctgcgacgg
      841 tacgctcgcc gttacccgcg gcgcctggca ggggccgaac tccaccagcg gttatttcac
      901 cacggtctgg cagcgccaga aggacggcaa gtacaaatgg gtgctcgatc agggcgagga
      961 gctgccgatg ccgctcgatc cgcccgacat gatcgtgggc aaggtggccg attgcccgcc
     1021 gggctatcgc cccgggcgcg gaccccggcc caaggatttc aagggcaacc tgccgccgct
     1081 cgatccggcg cgccgttcgg gccagtcgct cgatggcacc ctgggctggg acgtgaccgt
     1141 ctcgcccgaa ggggcccggc gctttaccgt gaccatgcag cttgagggcg cccgcgccac
     1201 ggtccaggac cttcaggtag aggcgcccgc gaagaagtga tgtgggaact gttcctatcg
     1261 gccttcgtca ccctgttcgt ggtgatcgat ccgcccggct gcgcgccgat ctatgccggc
     1321 ctggtcaagg gcgccaacaa ccgtctggca accgccatgg cggtgcgggc caccttcatc
     1381 gcggccatca tcctgctggt ctttgccctg ttcggggaag acctgctggg cgcgctccac
     1441 atcgagctta actcgttccg catcgccggc gggatcatgc tgttcgtgat cgcgatggac
     1501 atggtcttcg aaaagcgcac ccagcggcgc gaggagcggg ccgagaaggt ccgcgaggag
     1561 cacgtcgagg acgtttcggt cttcccgatg gccatgccga tgatcgcggg gccgggctcg
     1621 atcgccacga tcatgctgct gaccgcccgg gcccagggca ccgaacagac cctggtggtg
     1681 ctggcggcga tgggcgcggt cctgctgctc accctgcttg cgctggtcgc cgccggtccg
     1741 ctgatgaagc tgctcggcgc acgggtggaa gcggtgatca cccgcctgct cggcgtgctg
     1801 ctggccgcac tggcggcgca atacgtgatt gacgggatca aggcggtgct ggtcgccacc
     1861 tgaaggagaa ggcggacatg acggtggatc aggcccaggc cgcgctgcgg caggcgttcg
     1921 cgcacggcgg cccgggggcc gtggtctccg cactggtctg gttcgccgcc gccgcggcct
     1981 ttgtccgcac cgatcatgcc accgcctttg ccgtgctgtt cttcggcggg atgctgatct
     2041 tcccggtctc cagcctgatc ggaaagctcg ctttcgggcg cagcgcttcg gccaagggca
     2101 atccgctcgg gctgctcgcg ctggagagca cgatcgcgat gatcggctgt ctgatcgcgg
     2161 cctggctgct gctgaaggac cgccccgatt gggtcttccc cgttgccgcc atcgcggtcg
     2221 ggacgcatta tctgccgttc aagaccgcct atggcgaccg cacctattgg ctgctcggcg
     2281 cggccatcac cgtgatcggc tgtgccggga tcttcgttct gccggggcag accctggcgg
     2341 tgatcctggc cgtggccgtg accgagctgg tctttggcct ggccctgacc cgcgcgggaa
     2401 cccgcgtcta gcgcaggatc acgtcgacga tcgtccgggt gatcgcatcg accacgaaga
     2461 cccgcccgtc acggtagagg tagcggcgcc cgtcctgcgc gggatagcgg ctgcggtaga
     2521 aatccggcag cgcggcatag gcgatgaact gggcggccca ggcatcgggc agccgatccc
     2581 ccttgcgcca ctggcccggc ggcaggcagc cggtgttctt tttggcaagg cccggcgggc
     2641 agggtttggc ccgggcggcc ttgggcggct tcttggcagc aggtgcgtgg ccagcatggc
     2701 cggcgtgcga accgtggccg ttggcgttcc ctttcccggc ggcatgaacg gcccccggga
     2761 cggcaaaggc cagggcggcg gcagccaggg caagcggggc aaagcggaca gcaggcatgg
     2821 gcgtgtctcc agacagcggg gtcattgcgg catcctagcg cgcaatcgcc ggggcggccc
     2881 cctgaaaaaa cagccctatt gcagggtcac ctgcggatcg tcgctgtcgc gccggccaaa
     2941 gaactgcatc agctggacca gcagctcgca ccgcgtggcc agcgagggtg cttcgagcaa
     3001 cgcctgcttc gaggcaatgt cgaacgggac gatctggctg gtgccgttga tcagcgattc
     3061 gtcgtccagc cgggtcaccg cttcccaatc gaccgaatag ccctggctgt cggaaaagcg
     3121 gcgcgcctcg cgctcgaacc cggcgcgttc gaccggggag agcgcgggat agtcttcctc
     3181 gtccagcagc tcggcctcga tctggcggaa cggggtggcg gcatccagct cgcggaccag
     3241 gcggaaccgg gcctcgcctt ccagcagcag gttgaaccgc ccgtcgtcca acgcttcgac
     3301 ctcgccgatc ttgcccagac agccgatctg gtagagcggc gcgccttcct cggcccgctg
     3361 cggctggatc atgccgatcc ggcggtcgcg cgccagcgca tccttcacca gcgcccggta
     3421 gcgcggctcg aagatgtgca gcggcaactg cagccccggg aacaggatcg ctccgggcag
     3481 gggaaagatc gaaacgcgca tgaccta
//
LOCUS       DASUDV010000132         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_777177,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000132 DASUDV010000000
VERSION     DASUDV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777177"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFV30_02915"
     CDS             <1..771
                     /locus_tag="VFV30_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX4847072.1"
                     /translation="VVCHREAHIEVDEGGAPGFYLHGAKLMLAEGEGAKLTPAAIAAV
                     IDPIRNDVHQVQPHAVSITQASEYGRAYQPAEMTAIAAYCAERGLPLHVDGARFGNAV
                     AFLGCSPWEACQGAAALSFGMIKNGGMSAEALVFFDTGLADVARYRRKRAGHLQSKGR
                     YLAAQLLAMLEGDLWLENARHANAAAQELAGAAQDRLLHPVEANEVFLRLSAPEREAL
                     RAQGFSFYDWGDEAARMVTAWNSPMEHVTALARAIAAL"
     gene            768..1586
                     /locus_tag="VFV30_02920"
                     /pseudo
     CDS             768..1586
                     /locus_tag="VFV30_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(1580..2423)
                     /locus_tag="VFV30_02925"
                     /pseudo
     CDS             complement(1580..2423)
                     /locus_tag="VFV30_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
BASE COUNT          371 a          846 c          899 g          457 t
ORIGIN      
        1 gtggtctgcc accgcgaggc ccatatcgag gttgatgagg gcggggcacc aggcttctac
       61 ctccacggcg ccaagctgat gctggccgag ggtgaggggg ccaagctgac gccagcggcc
      121 attgcggcgg tgatcgatcc gatccgcaac gatgtccacc aggtgcagcc gcacgccgtt
      181 tcgatcactc aggcgagcga atatggccgt gcctatcagc cggccgagat gacggcgatt
      241 gccgcctatt gtgccgagcg cggcctgccg ctgcacgtcg atggcgcgcg gttcggcaac
      301 gcagtggcct tcctcggctg ctcaccatgg gaagcctgcc agggcgcagc ggcgcttagc
      361 tttggcatga tcaagaatgg cgggatgagc gccgaggcgc tggtgttctt tgacaccggc
      421 cttgccgatg tggcccgcta tcgccgcaag cgcgcggggc atctgcaatc gaagggccgc
      481 taccttgccg cccagctcct cgccatgctt gagggcgacc tgtggctgga gaacgcccgc
      541 catgccaatg ccgcggcgca ggagctggcg ggcgcggcgc aggaccgcct gctgcacccg
      601 gtcgaagcca acgaggtatt cctgcggctg tccgcgcccg agcgcgaggc gctgcgcgcg
      661 caaggcttct ccttctatga ctggggcgac gaggcggcgc gcatggtgac ggcctggaac
      721 agcccgatgg agcatgtcac ggcgctggcc cgcgcgatcg cggcgctatg agcggcgtga
      781 cgctgacgcc gcgctcgatc gcggcgttca tcttcctggc gctggtctgg ggctcgacct
      841 ggctggtgat caaggaccag atcagcgccg ttccgtcggc ctggtcggtg gtctggcgct
      901 ttatcctggc cgctaccgcc atgtcggcct tcgccctgct gcgcggcgaa cggatgcggc
      961 tgacggcacg cgaaatggct gtggcgattg ccatgggggt gttccagttc agcctcaact
     1021 tccagctgat ctaccagtcg gaacattacc tgacctcggg cctggtcgcg gtggtctttg
     1081 cgctgatgat cgtgcccaat gccgtgctgg cgcggctggt gctgggcgcg gcgctgacgc
     1141 ggcgcttcgt ggcgggatcg gcgattgccc tggcagggat cgccttgctg atgctgcatg
     1201 aataccgcat cgctccgccg aacagcggcg tgacgaccgg cgtcctgctg gtgacggggg
     1261 cgatcctctc ggtctcggtc ggcaacctcc tgcaggcgac gcaaaccgcg cgcgcggtgc
     1321 cggtggtctc gcttatggct tgggggatga tctggggcat cgtggcgagc gccgcctatg
     1381 cgatcgtcgc ggtcggcgcc ccggtgcttg atccgcgccc ggcctatctg gcggggatcg
     1441 cctacctcgg cctggtcggc tcggcgctga ctttcccgct ctattcgcag ctgatccgcg
     1501 actgggggcc ggggccggcg gcctataacg gcgttgccgt gccggtggtg gcgatgatcc
     1561 tttcgaccct gttcgaaggc tatgaatgga gcctgctggc agcgggcggg gcggtgctcg
     1621 ccatggccgg tctgctggta gcgctatcgg gtcggaagta ggctggccag cccctcgcga
     1681 taggtcggat agcgcgggct ccagcccagc acccgattcg ccttgccgtt cgcgacgcgg
     1741 cggttctcgg cataaaaggc ccgcgccatc ggcgagaggc cggcctcgtc cagcgtttgc
     1801 agcggcggct gcggcatgcc gagcagcgcg caggcgtgct cgatcacttc gttctggctg
     1861 cagggcagat catcgctgat gttgtaggcg cctgcggggg catcaagcgc cagcacggcg
     1921 gcgctgacga tatcgtcgac atggacgcgg ctgaacacct ggttgtccag cgcgatgcgg
     1981 tgggccttgc cttcttccac ccgctccagt gccgagcggc cggggccata gatccccggc
     2041 aagcgcagca cccgcgctcc gcgcgccagc cattcgcggt ccgcatcggc gcgcgcggtg
     2101 cggcggccgg tgccagtggg ggcgctttca tcgacccagg ccccggcggt atcgccatag
     2161 accccggtcg aggagagata ggtcagcggc ttgcccacca gggcatcgcc ataggtcagc
     2221 agtaccggat cgccatcggc cgagggcggg accgatgaca gtacctggtc ggcatcggcc
     2281 agcgccatcc gcaccgatcc ggcatcgtca aaccgcaacg tcccggcgct gcctgtcgag
     2341 accacttccc acccgcgcgc tgccatgaca ccggcaaagc gggcggcggt gtagccgagg
     2401 ccgaagatga acaggcgttt catcgagctc ccagttctcc tggttcgtca ccctgaactt
     2461 gtttcagggt ccatctggcc gatcaggcct tgcctcgcca gtcccaaacc gaggttgacg
     2521 tcaatcccgc tgtccgcggg cagtgcggca gaatggatgc tgaaacaagt tca
//
LOCUS       DASUDV010000133         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_865625,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000133 DASUDV010000000
VERSION     DASUDV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865625"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="ruvB"
                     /locus_tag="VFV30_02930"
     CDS             complement(<1..628)
                     /gene="ruvB"
                     /locus_tag="VFV30_02930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX4847073.1"
                     /translation="MTDNPLLSPARQAEDADAALRPKTLADFVGQAAAKDNLRVFIES
                     AKSRGEAMDHVLFFGPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTNLE
                     EGDVLFIDEIHRLNPQVEEVLYPAMEDRALDLIIGEGPSARSVRIDLPPFTLVGATTR
                     QGLLTTPLRDRFGIPVRLNFYTPEELVQIVQRGARLLGMPMAPDGAMEI"
     gene            complement(625..1317)
                     /locus_tag="VFV30_02935"
     CDS             complement(625..1317)
                     /locus_tag="VFV30_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4847074.1"
                     /translation="MLSPARRAAIFLAAMAAAAWPALALAQGFDLDCRQVPTEAGLTY
                     GPRKMHLQIDRAAGKWCADRCDTVEKLAAADGDRLTLLDGLTKLGDPVYRMVDLSTAA
                     MVDVFGVGKPWERKVHFICTPDTFGGFAPYLAADPEPIGNIGTKILAGGPPRGADGKM
                     LEGRVAFRIEVDAAGQPTACAITAASGTPALDERICPLVMPGLRFRPARDRAGKAVPG
                     RYESRVRWINPD"
     gene            1384..1719
                     /locus_tag="VFV30_02940"
     CDS             1384..1719
                     /locus_tag="VFV30_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX4847075.1"
                     /translation="MRQERLPCVYILASAHYGTLYIGVTSNLIARLWQHRNGALPGFT
                     SRHKVHRLVHYELFGEMEPAILREKQLKRWHRQWKINLINEGNPHWIDLAVGLGLPPL
                     ASGQPKNGP"
     gene            complement(1764..>2116)
                     /locus_tag="VFV30_02945"
     CDS             complement(1764..>2116)
                     /locus_tag="VFV30_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX4847076.1"
                     /translation="KMALAVLSALTVDELQRACAGGDAAMVARAQGVGPKLAGRIVNE
                     LKDKASALPSAAGAALASVPAGSASSDAVSALQNLGFKPNVATEAVARALEELGEGAE
                     LNALVRVALKRAAG"
BASE COUNT          349 a          744 c          682 g          341 t
ORIGIN      
        1 cgatttccat ggccccatcg ggcgccatcg gcatgccgag caggcgggcc ccgcgctgca
       61 cgatctgcac cagttcctcg ggcgtgtaaa aattgagccg caccggaatg ccgaagcggt
      121 cccgcagcgg cgtggtcagc agcccctgcc gggtggtcgc gccgaccagc gtgaacggcg
      181 gcagatcgat ccgcaccgag cgcgccgaag gcccctcgcc aatgatcaga tcgagcgcgc
      241 ggtcctccat cgccggatag agcacctctt caacctgcgg gttgaggcgg tgaatctcgt
      301 cgatgaacag gacgtcgccc tcttccagat tggtcagcag cgcggccaga tcgccggcct
      361 tggcaatcac cgggccgctg gtggcgcgaa agcccacgcc cagttcgcgc gcgacgatct
      421 gcgccagcgt ggtcttgccc agccccggcg ggccgaagaa cagcacgtga tccatcgcct
      481 cgccgcgcga cttggcgctt tcgatgaaga ccctgaggtt gtccttcgcc gcggcctgcc
      541 cgacaaaatc ggccagcgtc ttcggccgca gcgcggcgtc ggcgtcttcg gcctgccggg
      601 ccggggaaag gagcgggttg tcagtcaatc ggggtttatc cagcgcacgc gcgattcata
      661 gcgacccggc accgctttgc cagcccgatc ccgcgccgga cggaaccgca gccccggcat
      721 gaccagcggg cagatccgct cgtccagcgc gggcgttccg ctggccgcgg taatcgcgca
      781 agcggtgggt tggccggcgg cgtccacctc gatccggaag gccaccctgc cttccagcat
      841 cttgccgtcc gcacctcttg gcgggccgcc agcgaggatc ttggtgccga tattcccgat
      901 cggctcggga tcggcggcca gatagggcgc gaagccgccg aaggtgtcgg gcgtgcagat
      961 gaagtggacc ttgcgttccc acggcttgcc caccccgaaa acgtcgacca tcgcggcggt
     1021 cgacaagtcg accatccggt agaccgggtc gcccagcttg gtcagcccgt ccagcagagt
     1081 gagcctgtcg ccgtctgccg cggccagctt ttcgaccgtg tcacagcgat cggcgcacca
     1141 cttgcccgca gcgcgatcga tctggaggtg cattttgcgc gggccgtagg tgagccccgc
     1201 ctcggtcggc acctgccggc aatcgagatc gaagccctgc gccagggcca aagccggcca
     1261 ggcggcggca gccatggcag cgaggaagat cgcggcgcgg cgggcggggg aaagcatcca
     1321 attcaagcca ctttcgtcac cctgaacttg tttcagggtc catcgtgccg cacagtcgga
     1381 tttatgcggc aagaacgcct gccctgcgtc tacatccttg cgagcgcgca ctacggcacg
     1441 ctgtacatcg gcgtcacatc caacctgatc gcccgcctct ggcagcaccg caacggtgcc
     1501 cttcccggct ttacttcgcg ccacaaagtc caccggctgg ttcattacga actgttcggc
     1561 gaaatggagc ccgcgatctt gcgtgaaaag cagctgaagc gctggcaccg gcaatggaag
     1621 atcaatctca tcaacgaggg aaacccgcat tggatcgacc ttgcggtggg cttgggcctg
     1681 ccgccgcttg cttcgggcca accgaagaat gggccctgaa acaagttcag ggtgacgggc
     1741 ttgaggggaa cgggagtacc tgttcacccc gccgccctct tcagcgccac ccgcaccagc
     1801 gcgttgagct ctgccccctc gcccagttcc tccagcgcgc gggcgacggc ttcggtggcg
     1861 acgttgggtt tgaagcccag gttctgcaaa gcggagaccg cgtcgctgct ggccgatccg
     1921 gccggaaccg aggccagcgc cgcgccggcc gcgctgggca acgcgctggc cttgtccttc
     1981 agttcgttga cgatccgccc ggccagcttc ggcccgacgc cctgcgcccg ggcgaccatc
     2041 gccgcatcac cgcccgcgca ggcgcgctga agttcgtcca cggtcagcgc ggagagcacc
     2101 gccagcgcca tcttcg
//
LOCUS       DASUDV010000134         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_755129,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000134 DASUDV010000000
VERSION     DASUDV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755129"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFV30_02950"
     CDS             complement(<1..544)
                     /locus_tag="VFV30_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847077.1"
                     /translation="MSARFPILVLALLLAACGDGESSADSSDRDPAIVGALSDPIMAD
                     PDLASQNRGNSALQGGGPPSLEVPPFRSDPEEAELAKAQALELAGGRIAALPPEGERL
                     DTSRLGTALSAAAVAEALPELGKGCGKALQYGFIWAARLPAALPVYPRGHARQAAGSD
                     SAACRIRVVNFVTPVSAEDVL"
     gene            792..1700
                     /gene="rarD"
                     /locus_tag="VFV30_02955"
     CDS             792..1700
                     /gene="rarD"
                     /locus_tag="VFV30_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX4847078.1"
                     /translation="MQQPQPQSASGGLAMALGAHLVWGLLPLYLKLVHAVPPVEFVGW
                     RIVFALPFCLLFILLRRQLGELWAILRSLKTMRALAASALLIAANWLIYVFAIQTGHV
                     YAASFGYYVSPLMQVLAGTLFLGERLSPRQWWAVALAGLGVAVLGWGELSMLWISLAL
                     AASWSGYGLIRKLVPVGSLPGLTVEAMVLIPFAAGYLAWLGAGPTRLSFGHELGMSSL
                     IVLAGPLTALPLTLFAIAARRLKFSTLGMLQFTSPTVVFLLGATVFGLPLEVGQLASF
                     AIIWAAIAMFLWDLVAPRRLAEKTPA"
     gene            complement(1677..>2045)
                     /locus_tag="VFV30_02960"
     CDS             complement(1677..>2045)
                     /locus_tag="VFV30_02960"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070161.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4847079.1"
                     /translation="KREVHRQALRKAATGGSAKFFLGTDSAPHAVGAKEAACGCAGIF
                     NAPFALESYLAVFEEEGAIDKFEAFASLNGPAFYGLAVNEATVTLEKTPVEVAAQIDA
                     NGTPVVPFHAGETLGWRFLG"
BASE COUNT          314 a          681 c          690 g          360 t
ORIGIN      
        1 ccagcacatc ttcggcgctg accggggtga cgaagttcac cacccggatg cggcaggcgg
       61 cgctgtcgct tcccgcggcc tggcgggcat gaccgcgcgg atagaccggc agtgccgccg
      121 gcaggcgcgc cgcccagatg aaaccgtatt gcaaggcctt gccgcaaccc ttgcccagtt
      181 cgggcaaggc ttcggcaacc gccgccgcgc tcagcgccgt gccaagccgc gatgtgtcga
      241 ggcgctcgcc ttcgggcggc agcgcggcaa tcctgccacc ggccagctcc agcgcctggg
      301 ccttggccag ttccgcctct tcgggatcgg agcgaaacgg gggaacctcc agcgaaggcg
      361 gcccgccgcc ctgcaaggcc gaattgccgc ggttctggct ggcgagatcg ggatcggcca
      421 tgatcggatc ggacaaggca ccgacgatcg ccggatcgcg gtcactgcta tcggcgctgc
      481 tttcgccatc gccgcaagca gccagcagca gggccaggac aaggatcggg aagcgcgcag
      541 acatcgtcgc aacaccatgg cgcaagcgaa ggccaaggcc aagaactgct ttgggcgcgc
      601 cttcggggag aaatcgaagg cgcgcccggc agcagggtga aaacgcgacc caccgctgca
      661 gtgcccgggc cggcgcgagg gagggaggga tcgcctgcac cagagcttga tggagtgttt
      721 accacggcaa acctgaaccc acggcaacgc gaagcttgcc ctgggttgct tcaccatcta
      781 gcccgtccgc gatgcagcaa ccccaacccc aatcggccag cggcggcctt gcgatggcgc
      841 tgggcgcgca tctggtatgg ggcctgttgc cgctttatct gaagctggtc cacgcggtgc
      901 cgccagtgga attcgttggc tggcggatcg tcttcgcctt gcccttctgt cttctgttca
      961 tcctcctgcg ccgccagctg ggcgaactgt gggcaatcct gcgcagcctg aaaaccatgc
     1021 gggctctggc agcaagcgcc ctgctgatcg cggccaactg gctgatctat gtgttcgcga
     1081 tccagacggg ccacgtctat gccgccagtt tcggctatta cgtatcgccg ctgatgcagg
     1141 tgcttgccgg cacgctgttc ctgggcgaac gcctctcgcc ccggcagtgg tgggcggtgg
     1201 cactggccgg gctgggcgtt gcggtgctgg gctggggcga actttcgatg ctgtggatca
     1261 gcctggcgct ggccgcaagc tggtcgggct atgggctgat ccgcaagctg gtgccggttg
     1321 gctcattgcc gggactgacg gtcgaggcga tggtgctgat ccccttcgcg gcgggctatc
     1381 tggcctggct tggcgccggg ccgaccaggc tcagcttcgg gcacgaactt ggcatgtcga
     1441 gcctgatcgt tctggccggc ccgctcaccg cgttgccgct gacgctgttc gcgatcgccg
     1501 cgcggcgctt gaagttcagc acgctcggaa tgctgcagtt cacctcgccc acggtggtgt
     1561 tcctgctggg ggcaacggtg ttcggcctgc cgctggaagt gggccagctg gccagcttcg
     1621 cgatcatctg ggcggcgatc gccatgttcc tgtgggacct ggtggccccg cgccggctag
     1681 ccgagaaaac gccagcctag cgtttcgcca gcatggaacg gcacaaccgg ggtgccgttg
     1741 gcatcgatct gcgccgctac ctcgaccggg gtcttttcca gggtcaccgt cgcttcgttg
     1801 accgcaagcc catagaatgc cgggccattg agcgaggcga aagcctcgaa cttgtcgatc
     1861 gccccttcct cttcgaacac ggcaaggtag gattcgagcg caaagggcgc gttgaagatc
     1921 cccgcgcagc cgcaggccgc ttccttggcc cccaccgcat gcggggcgct gtcggtgcca
     1981 aggaagaact tggccgagcc ccccgtcgcc gccttgcgca gcgcctgccg gtgcacttcg
     2041 cgctt
//
LOCUS       DASUDV010000135         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_92405,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000135 DASUDV010000000
VERSION     DASUDV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92405"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFV30_02965"
     CDS             complement(<1..1236)
                     /locus_tag="VFV30_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX4847080.1"
                     /translation="MAFGSADPTSRLKAVLGPTNTGKTHLAIERLCAHSSGMIGFPLR
                     LLAREVYDKVCAIKGADRVALITGEERIEPKNARWLLCTAEAMPVGADVAFAALDEVQ
                     LGADRERGHIFTDRLLHARGREETMLLGSATAEPLVRALLPEAEIVSRPRFSTLSHAG
                     AKKLSRIPPRSAIVAFSAEQVYAVAEMLRRFRGGAAVVMGALSPETRNRQVALYQQGE
                     VDYLVATDAIGMGLNLDIDHVAFAGLSKFDGIRQRRLTTSEMAQIAGRAGRHQRDGTF
                     GTLAGSGSHDAEFTAEEVYAIEEHRFPPLTRLFWREAEPDSASLGALIADLARPPARE
                     GLAAAPEAIDLAVLRRLAEDPEIAATVRGRSQVERFWQVCSLPDFRQHGAETHARFVA
                     RLWQDLARGQLGGDFVARAI"
     gene            1388..2959
                     /locus_tag="VFV30_02970"
     CDS             1388..2959
                     /locus_tag="VFV30_02970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4847081.1"
                     /translation="MVKPREVPHTGQGGSVLTVRAEGVAVRSASGSWLDRFEQRRAEW
                     AARIEGIDWAPDLAEDIGSATWFRGLGTLVGLSLAALAFWPEGDAIAAAPAMPLDASA
                     RDEFRSQMIQPLAFGADSGRRMGATKAVTPLASAPGRSTVQLTATLAQGDSFLRMLTR
                     AGVSEADAGAVTQLVAAQLPVSLIAPGTQVDLRLGAPDPATGVRPLEHAAFRARFDLD
                     LSIDRTDGQLRLSAHPIAVDTTPMRIRGVVGTSLYRSARAAGAPIEAIQQYLETLDQH
                     VSLDTAIEAGDTFDIIVAAKRSANGEVEVGDLLYAGLERGGRNKAQLVRWGSEGQFFE
                     ASGMGAQRTGLIMPVYGGRVTSGFGARRHPVLGYTRMHAGIDFGAPHGAPIYAVGDAT
                     VIFSGWHGGHGNYVKLDHGGGYATAYAHMSRIAVPSGQRVRAGQVIGYVGSTGLSTGP
                     HLHYELYRNGQAVNPLSVSFTVSSQVDAKELAAFRARLAALKTIKPGAAMTSLGPRQA
                     ATAQTAGREIDRLKS"
     gene            2991..>3890
                     /gene="hemB"
                     /locus_tag="VFV30_02975"
     CDS             2991..>3890
                     /gene="hemB"
                     /locus_tag="VFV30_02975"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4847082.1"
                     /translation="MTQFPATRLRRTRASAWSRALHRETVLTPADLIWPLFVTEGSGV
                     EEPIGSLPGVSRWSVDRIAERAKEAVALGIPCLALFPNTQADRRSEDGAEALNPDNLM
                     CRAIRTIKQACGEDIGVLTDVALDPYTSHGQDGLIDAAGYVLNDATVDVLVGQSLNQA
                     AAGADVIAPSDMMDGRIGAIRDALESAGHANVQIMSYAAKYASAFYGPFRDAVGSRGL
                     LKGDKKTYQMDPANAEEALREVELDLAEGADSVMVKPGLPYLDIVRKVKDTFAVPVFA
                     YQVSGEYAMIEHAAAAGAGDRDAL"
BASE COUNT          596 a         1356 c         1371 g          567 t
ORIGIN      
        1 aatggcgcgg gcgacaaagt ccccgcccag ctggccgcgg gccaggtcct gccacagccg
       61 ggccacgaac cgggcgtggg tttcggcccc gtgctggcgg aaatcgggca gcgagcagac
      121 ctgccagaac cgctccacct gggagcggcc gcgcacggtt gcggcaatct cgggatcctc
      181 ggccaggcgg cgcaggacgg cgagatcgat cgcttcgggc gcggcggcca gcccttcgcg
      241 cgcgggcggg cgggccaggt cggcgatcag cgcgcccagg ctggcgctgt cgggttcggc
      301 ttcgcgccag aacagcctgg tcagcggcgg gaagcggtgc tcctcgatcg cgtagacctc
      361 ctcggcggtg aactccgcgt cgtggctgcc gctgccggcc agggtgccga aggtgccgtc
      421 gcgctggtgc cgcccggcgc ggccggcaat ctgggccatt tcgctggtgg tcaggcggcg
      481 ctggcgaatc ccgtcgaact tgctcagccc ggcaaaggcg acatgatcga tatcgaggtt
      541 gagccccatg ccgatcgcat cggtggcgac caggtaatcg acctcgcctt gctggtacaa
      601 tgctacctgc cggttgcggg tttctggcga aagcgcgccc atcaccaccg ccgcgccgcc
      661 gcggaaccgg cgcagcatct cggccacggc gtacacctgc tcggcgctga aggcgacaat
      721 cgcgctgcgc ggggggatcc ggctgagctt ctttgcgccg gcgtggctga gcgtggaaaa
      781 gcgcgggcgc gagacgattt cggcctcggg cagcagcgcg cggaccaagg gctcggccgt
      841 ggccgaaccc agcagcatgg tctcctcgcg cccccgggcg tgaagcagcc ggtcggtaaa
      901 gatgtggccg cgttcgcggt cggcccccag ctgcacctcg tccagcgcgg caaaggcgac
      961 atcggcgccg accggcatgg cctcggccgt gcacagcagc cagcgcgcgt tcttcggctc
     1021 gatccgctct tccccggtga tcagcgccac ccggtccgcg cccttgatcg cgcagacctt
     1081 gtcatagacc tcgcgcgcca gcagccgcag cggaaagccg atcatgcccg aggaatgggc
     1141 gcacagccgc tcgatcgcca aatgggtctt gcccgtgttg gtcgggccga gcacggcctt
     1201 gagccgcgaa gtgggatcgg cggaaccgaa agccatgacc cctgcgatgt ggcaaggccg
     1261 gcgggccgcc gcaaggtggg cggggcaaag attcccccgc ggtctgaaaa atccatgcgg
     1321 acttagccga agattaacca tgccgcgggc aagagggggc acgttcaagc gctcttgggg
     1381 gcaggaattg gtcaagccgc gcgaagtgcc gcacaccggg cagggcggat ccgttttgac
     1441 ggtccgcgcc gagggcgttg ccgtgcgctc tgcatcgggc agctggctgg accgcttcga
     1501 acagcgccgc gccgaatggg ccgcccggat cgaggggatc gactgggccc cggacctcgc
     1561 cgaagacatt ggcagcgcta cctggtttcg cggcctcggc accctggtgg ggctgtcgct
     1621 cgcggccctg gccttctggc cggaaggcga tgcgatcgcc gccgcgccgg ccatgccgct
     1681 ggatgccagc gcccgcgacg aattccgcag ccagatgatc cagccgctcg ctttcggcgc
     1741 cgacagcggc cgccggatgg gcgcgaccaa ggcggtcact ccgctggcct cggccccggg
     1801 ccgcagcacc gtgcaactga ccgcaaccct ggcccagggc gacagcttcc tcaggatgct
     1861 gacccgcgcc ggggtatccg aagccgatgc cggcgccgtg acgcagctgg tcgccgcgca
     1921 attgccggtc tcgctgatcg ctccgggcac gcaggtggac ctgcggctgg gcgcgccgga
     1981 tccggccacc ggggttcgcc cgctggagca cgcggccttc cgcgcccgct tcgatctcga
     2041 tctgtcgatt gaccgcaccg acgggcagct gcggctgtcg gcccacccga tcgcggtgga
     2101 taccaccccg atgcggatcc gcggcgtggt cggcaccagc ctctaccgct cggcccgcgc
     2161 cgccggcgcg ccgatcgagg cgatccagca gtacctggaa acgcttgacc agcacgtcag
     2221 cctcgacacc gcgatcgagg ccggggacac ctttgacatc atcgttgccg ccaaacgttc
     2281 cgccaacggc gaagtggaag tgggcgatct gctctatgcc gggctggagc gcggcggccg
     2341 caacaaggcc cagctggtcc gctggggttc ggaaggccag tttttcgaag cctcgggcat
     2401 gggcgcccag cgcaccggcc tgatcatgcc ggtctatggc ggccgggtga cttcgggctt
     2461 cggcgcgcgg cgccacccgg tgctgggcta tacccggatg cacgccggga tcgatttcgg
     2521 cgcgccgcac ggggcgccga tctatgcggt cggcgatgcc acggtgatct tctccggctg
     2581 gcacggcggc cacggcaact atgtgaagct cgatcacggc ggcggctatg ccacggccta
     2641 tgcccacatg agccggatcg cggtgccttc gggccagcgg gtccgcgccg ggcaggtgat
     2701 cggctatgtc ggctcgaccg gcctctcgac cgggccgcat ctgcactatg agctgtaccg
     2761 caacggccag gcggtcaacc cgctctcggt cagcttcacc gtctcctcgc aggtcgacgc
     2821 caaggaactg gcggccttcc gggcgcggct ggcggcgctc aagaccatca agcccggcgc
     2881 ggcgatgacc agccttggcc cgcgccaggc ggcgaccgcc caaaccgcgg gccgcgagat
     2941 cgaccgactc aagagttgag ccggggtccg ccccggccta tgagccgggc atgacccagt
     3001 ttcccgccac ccgcctgcgc cgcacccgcg cctctgcgtg gagccgcgcg ctgcaccgcg
     3061 aaaccgtgct gaccccggcc gacctgatct ggccgctgtt cgtcaccgaa ggcagcggcg
     3121 tggaagagcc gatcggttcg ctgcccggcg tctcgcgctg gtcggtcgac cggattgccg
     3181 agcgggcgaa ggaagcggtg gcgctgggca ttccctgcct ggcgctgttt cccaacaccc
     3241 aggccgaccg gcgcagcgag gacggggccg aggcgctcaa ccccgacaac ctgatgtgcc
     3301 gggcgatccg gacgatcaag caggcctgcg gcgaggacat cggcgtgctg accgatgtcg
     3361 cgctcgatcc ctataccagc cacgggcagg acgggctgat cgatgccgcc ggctatgtcc
     3421 tcaacgatgc cacggtggac gtgctggtcg gccagagcct caaccaggcg gcggccgggg
     3481 ccgatgtgat tgcccccagc gacatgatgg acggccggat cggcgcgatc cgcgacgcgc
     3541 tggaatcggc cggccacgcc aatgtccaga tcatgtccta cgcggcgaaa tacgccagcg
     3601 ccttctacgg cccgttccgt gacgcggtcg gcagccgcgg actgctcaag ggcgacaaga
     3661 agacctacca gatggatcct gccaacgccg aggaagcctt gcgcgaggtc gagctggacc
     3721 tggccgaagg ggccgacagc gtgatggtca agcccgggct gccctatctc gatatcgtac
     3781 ggaaagtgaa ggatactttc gccgtcccgg tattcgctta ccaggtcagc ggggagtatg
     3841 cgatgatcga gcatgccgcc gcggccgggg cgggggaccg cgatgcgctg
//
LOCUS       DASUDV010000136         3482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1219265,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000136 DASUDV010000000
VERSION     DASUDV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219265"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFV30_02980"
     CDS             complement(<1..1244)
                     /locus_tag="VFV30_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847083.1"
                     /translation="MSRSDPAPAGADPTGTATLPTGRLVLLFTVSLVTAAGNTAMQSV
                     MPSIGTALKVHDFWISLAYTWSALLWMTCAPMWARRSDRRGRKAMMGIGLTGFIASFG
                     LCGLALWLGLNGWLSAVGTLVLFALCRSLYGGFGSAAPPAVQAYVASRTSRAERTKSL
                     SLISSSFGLGTVIGPTLAPFFILPGLGLVGPFAAFTLIAVVVLIALRIHLPDDTPAYA
                     ARGDVIAAPFSANSDSRPMPNAAEQDNALPDAASIPKLDWFDKRLRPWLVAGLLGGHA
                     QAAVMGLVGFLVLDRLGLRADPAAGAGPVGLVMTAGAMATLLAQWGLIPILHLGPRAS
                     CLIGIGLAALGTAMLAFAHELHAITIAVGIISLGFGLFRPGFTAGASLAVSRAEQGQS
                     AGIVASVNGAAYIVAPAVGVWL"
     gene            1340..1966
                     /locus_tag="VFV30_02985"
     CDS             1340..1966
                     /locus_tag="VFV30_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4847084.1"
                     /translation="MTTTDLMQSGLGQRIKRRIKRALGPWGVFLQGFAAHPVMVGSII
                     PSSRFTVARMLKPVKWDECRLFVEYGPGVGTFCRPVLERLRRDGMLIVIDTNPLYIDY
                     LKATIADSRFVPVLGSAADVEAIVRAHGHDHADYVLSGLPFSTLPDGVGPAIAAATHR
                     VLRPGGAFLVYQFSAKARAFMARHFGRIDQGFEALNVLPCRLYWGWKD"
     gene            complement(1971..2783)
                     /locus_tag="VFV30_02990"
     CDS             complement(1971..2783)
                     /locus_tag="VFV30_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847085.1"
                     /translation="MNQPPATRPRFDSNRAWQEGSSAVAGNRDALLALAGVFIVVPTF
                     ALTTLAPFPAVEQGKDATHLLEALGVYFDEHWLAVSAVIVAQFIGTLCMLALISERSR
                     PTVSEAIRLAFRFAPPFLAGMALIMLAMPIIASVPGALLGISGVAALSLLGLGAGFVL
                     AFYGLSRLSLFGPAVLTEQRFNPFAALRRSLELTRDNGWRLTTFLVLLTLAFRLVGLL
                     IVAAASLLTTVAAGPESAAVVAAFFDSVMQGAYFTFLVAVLSAAYRQLAEPR"
     gene            2826..>3482
                     /gene="lipB"
                     /locus_tag="VFV30_02995"
     CDS             2826..>3482
                     /gene="lipB"
                     /locus_tag="VFV30_02995"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX4847086.1"
                     /translation="MDSALPVSIELRQESAPVPYREALEAMTLRNAAIAEGTAKELLW
                     LLEHPPVYTAGTSAAAGELLDPRFEVVEAGRGGRYTYHGPGQRVGYVLLDLKRRARDV
                     RGFVHAIEGWVIDTLGDFGVEAWRAPGRIGIWTRDIDGREAKIGAIGVRVRRWVTMHG
                     FSVNLDPDLSHFGGIVPCGIEEFGVTSLQRLGLAVSPAAWDAALLARAGAFLAALEQP
                     C"
BASE COUNT          582 a         1181 c         1249 g          470 t
ORIGIN      
        1 tagagccaga ccccgaccgc cggggcgacg atataggccg cgccgttgac cgaggcgaca
       61 atcccggccg actggccctg ctcggcccgg ctgaccgcca gcgaggcccc cgcggtaaag
      121 ccgggacgga acaggccgaa acccagcgag atgatgccga ccgcgatggt gatcgcgtgg
      181 agttcatggg cgaaggccag catcgcggtg cccagcgcgg cgaggccgat gccgatcagg
      241 cacgaggccc gcggccccag gtgcaggatc gggatcagcc cccactgcgc cagcagggtg
      301 gccatggccc cggcggtcat caccaggccg accggcccgg ccccggcagc gggatcggcg
      361 cgcagcccca gccggtccag caccagaaag ccgaccagcc ccatcaccgc cgcctgggca
      421 tggccgccca gcagcccggc gaccagccac ggccgcagcc gcttgtcgaa ccagtccagc
      481 ttggggatcg aagcggcatc gggcagggcg ttgtcctgct cggccgcgtt gggcatcggc
      541 cggctgtcgg aattggcgct gaacggggct gcgatcacat cgccgcgcgc ggcataggcc
      601 ggggtgtcgt cgggcaggtg gatccgcagc gcgatcagca ccaccaccgc gatcagggtg
      661 aaggccgcga aggggccgac caggcccagc cccggcagga tgaagaacgg ggccagggtc
      721 gggccgatca ccgtgcccag gccaaagctg gacgagatca gcgagagcga cttggtccgt
      781 tccgcccggc tggtgcggct ggcgacatag gcctggaccg ccggcggcgc ggccgaaccg
      841 aacccgccgt agagcgagcg gcacagcgca aacagcacca gcgtgccgac ggcgctgagc
      901 cagccgttga ggcccagcca cagcgccagc ccgcacagcc cgaagctggc gatgaagccg
      961 gtcagcccga tgcccatcat cgccttgcgc ccgcgccggt cggaccggcg ggcccacatc
     1021 ggcgcgcagg tcatccacag cagcgccgac caggtgtagg ccaggctgat ccagaaatcg
     1081 tggaccttca gcgccgtgcc gatcgagggc atgaccgatt gcatcgcggt gttgccggcg
     1141 gcggtcacca gcgaaacggt gaacagcagc accagccggc cggtgggcag ggtggccgtg
     1201 ccggtcgggt cggctccggc gggggcggga tcgctgcgag acatcgattc cccgctaagc
     1261 ctgtcgctct tgccttgcaa tggggcggca aatttgtgaa gggcggggcc cgttatcagc
     1321 agcagggcta atgattccca tgaccacgac tgatttaatg cagtccggcc tcggccagcg
     1381 gatcaagcgg cggatcaagc gggcactggg gccgtggggc gtgttcctgc agggctttgc
     1441 cgcgcacccg gtgatggtcg gatcgatcat cccgtcctcg cgcttcacgg tcgcccggat
     1501 gctgaagccg gttaaatggg acgaatgccg cctgttcgtg gaatacggcc ccggcgtcgg
     1561 caccttctgc cgcccggtgc tggagcggct gcggcgcgac ggcatgctga tcgtgatcga
     1621 caccaacccg ctctatatcg actacctgaa agcgacgatc gcggattcgc gcttcgtgcc
     1681 ggtgcttggc tcggccgccg atgtggaggc gatcgtgcgc gcccacgggc acgaccatgc
     1741 cgattacgtc ctctccggcc tgccgttctc caccctgccg gacggggtcg gcccggcgat
     1801 tgccgcggca acccaccgcg tgctgcgccc gggcggcgcg ttcctggtct atcaattcag
     1861 cgccaaggcc cgcgcattta tggcccgcca ttttggccgg atcgatcagg gcttcgaggc
     1921 gctcaacgtg ctgccctgcc ggctttactg gggctggaag gactgaagcc ctagcgcggc
     1981 tcggccagct ggcggtaggc ggcagacagg accgcgacca ggaaggtgaa ataggccccc
     2041 tgcatcacgc tgtcgaagaa cgccgcgacc acggctgccg attccggccc ggcggcaacg
     2101 gtggtgagca ggctggccgc cgccacgatc agcaggccga ccagccggaa ggccagggtc
     2161 agcagcacca gaaaggtggt cagccgccag ccgttgtcgc gggtcagttc cagcgagcgg
     2221 cgcagcgcgg caaagggatt gaaccgctgc tcggtcagca cggccgggcc gaacagcgac
     2281 agccgcgaca ggccgtagaa cgccaggaca aagcccgccc ccagccccag caggctgagc
     2341 gcggcaacgc ccgagatgcc cagcagcgcg ccgggcacgc tggcgatgat cggcatggcc
     2401 agcatgatca gcgccatccc ggccaggaac ggcggggcga agcggaaggc cagccggatc
     2461 gcttcgctca cggtcgggcg gctgcgttcg gaaatcagcg ccagcatgca cagcgtgccg
     2521 atgaactggg cgacgatcac ggccgaaacg gccagccagt gctcgtcgaa atagaccccc
     2581 agcgcctcga gcaggtgggt ggcgtccttg ccctgttcga ccgccggaaa cggggccagc
     2641 gtggtcaggg caaaggtcgg cacgacgatg aacaccccgg ccagcgccag cagcgcgtcg
     2701 cggttgccgg caacggcgga ggaaccctcc tgccaggcgc ggttgctgtc aaaacgcggg
     2761 cgggtggcgg ggggctggtt catgccccct tgataaccac agcatttgcc gccacgcaac
     2821 gtgcgatgga ttccgccctg cccgtttcga tcgaattgcg ccaggaaagc gccccggtcc
     2881 cctaccggga ggcgctggag gcgatgacgc tgcgcaacgc cgcgatcgcc gaagggaccg
     2941 cgaaggaact gctgtggctg ctggagcatc cgccggtcta caccgccgga accagcgcgg
     3001 cggcgggcga actgctcgat ccgcgcttcg aagtggtcga ggccgggcgc ggcgggcgct
     3061 atacctatca cgggccgggc cagcgggtcg gctatgtcct gctcgacctg aagcgccgcg
     3121 cgcgcgacgt gcgcgggttc gtccacgcga tcgagggttg ggtgatcgac accctgggcg
     3181 atttcggagt ggaggcctgg cgcgcgccgg gccggatcgg gatctggacc cgcgacattg
     3241 acgggcgcga ggccaagatc ggcgcgatcg gcgtgcgagt gcggcgctgg gtgacgatgc
     3301 acggcttctc ggtcaacctc gatccggacc tctcgcactt tggcgggatc gtgccctgcg
     3361 ggatcgagga attcggcgtg accagcctgc agcggctggg gcttgcggtc agtcccgccg
     3421 cgtgggatgc cgcgctgctg gcgcgcgccg gggctttcct ggcggcgctt gaacaacctt
     3481 gc
//
LOCUS       DASUDV010000137         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1108626,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000137 DASUDV010000000
VERSION     DASUDV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108626"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFV30_03000"
     CDS             <1..387
                     /locus_tag="VFV30_03000"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071449.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX4847087.1"
                     /translation="QVSGDSGANLKKRMRTGTVATTDDRNWELQYTQSAQRLSLSRAI
                     GIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEEAIAAHLKIGTT
                     ACDLLRAEGERLHSRKLRAFNEPPFR"
     gene            384..887
                     /locus_tag="VFV30_03005"
     CDS             384..887
                     /locus_tag="VFV30_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847088.1"
                     /translation="MIRRARLDDLSALPAVEFSAATAFRAIPDRAWLADDEAISHAEH
                     IALRERGITLVADGDGGICGFICSEPLGESLFIREVSVRHQDQGKGIGHGLLRALIDQ
                     ARAAGWTRITLTTFRDLAWNAPFYARLGFRELQADALAPELRLHLQREIEHGLPAEHR
                     CAMALDI"
     gene            complement(884..1642)
                     /locus_tag="VFV30_03010"
     CDS             complement(884..1642)
                     /locus_tag="VFV30_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4847089.1"
                     /translation="METEFYPGFDGAQLALHRMAPTSGSGRPLILLHGLFSTAEMNWI
                     KWGHAALLAEAGFECLMPDLRAHGHSAAPHDPAAYPDDVLAKDLAALVAHLGLEDYDL
                     CGFSLGARTSVRGVIGGLRPARLALGGMGLEGLAGWTGRGAFFIDAIDRFDEAKPGDP
                     IFPAVSFMKTMKVDRVATRLLLQTFADTPPKALAALAMPTLIVNGDQDRDNGDPEALT
                     RALPNAVQVIVPGTHMSCVTKPDLGLAIRDFLTA"
     gene            complement(1652..2083)
                     /locus_tag="VFV30_03015"
     CDS             complement(1652..2083)
                     /locus_tag="VFV30_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847090.1"
                     /translation="MKLELRPCAGEAELSDPAVTGAFAEQMAALSAQAQVPPWCGYIG
                     WQGNLPLGFGGFVGEPDGDGVIEIGYLTFPAHEGRGVASAVAAEMIAIARAQGLAAVI
                     AHTLPQENASTGVLRRNGFARDGEAQDPDEGTVWRWRLHLR"
     gene            complement(2080..2526)
                     /locus_tag="VFV30_03020"
     CDS             complement(2080..2526)
                     /locus_tag="VFV30_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX4847091.1"
                     /translation="MTQTMTGGCQCGRVRYSAEIDGDEAYWCHCRKCQKATGGIAAAF
                     VQVEPAKVTWLTEPDWYESSPVARRPFCSKCGTPLGFAWANGSGEMDLTVGSFDDPTR
                     FRPAAHAGCESMHDAWLDTRDLPRFYSASTPSVVKQWQDAGLEVPE"
     gene            complement(2536..3147)
                     /locus_tag="VFV30_03025"
     CDS             complement(2536..3147)
                     /locus_tag="VFV30_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX4847092.1"
                     /translation="MIQRRLFALIALAIPLSAFGARYPENTWLQVGPVALLLPAAWWA
                     MRRYPISDAAAGCLAAFVLLHLFAARWSYSFVPYDHWLPGLGSAFGFERNMFDRLVHF
                     LFGVLAVLPFAEIATRHGGLSLRAALWGAMAFVLAVGGLYEIFEWTLTLALAPEDAGA
                     YNGEQGDRFDAQKDMAIAAFGAILALIPVRRLLQGDGNDPATR"
BASE COUNT          564 a         1131 c         1076 g          494 t
ORIGIN      
        1 caggtcagcg gcgacagcgg cgccaacctg aagaagcgga tgcgcaccgg cacggtcgcc
       61 accaccgacg accgcaactg ggaactgcaa tacacccagt cggcccagcg cctgtcgctc
      121 agccgggcga tcgggatcga catggaaagc gccaccatcg ccgcccaggg ctatcgcttc
      181 cgcgtgccct acgggacgct gctgtgcgtt tcggacaagc cgctgcacgg cgagatcaag
      241 ctgccgggcc aggccaaccg cttctacgaa gaggcgatcg ccgcccacct caagatcggc
      301 accaccgcct gcgatctgct gcgggccgag ggcgagcggc ttcactcccg caagctgcgc
      361 gccttcaacg aaccgccgtt ccggtgatcc gtcgggcgcg gctcgacgat ctgtccgcgc
      421 tgccggcggt cgaattctcg gccgccactg cctttcgggc cataccggac cgggcctggc
      481 tggccgatga cgaagccatc tcgcacgccg agcacatcgc gctgcgggag cgtgggatta
      541 cgctggtagc ggacggcgat ggcgggattt gcggctttat ctgttccgag ccgctgggcg
      601 aaagcctgtt cattcgcgaa gtctcggtcc gccatcagga tcagggcaaa ggcatcgggc
      661 atggcctgct gcgcgccctg atcgatcagg cccgtgcggc cggctggacc cggatcacgc
      721 tcaccacctt tcgcgatctg gcctggaacg ctccgttcta tgcccggctc ggtttccggg
      781 agctgcaggc agacgcgctc gctccggagc ttcgcctgca cttgcagcgc gaaatcgagc
      841 atggccttcc cgccgaacac cgctgcgcga tggcgctgga catctaggcg gtcagaaagt
      901 cccggatcgc caggccaagg tccggcttgg tcacgcagct catgtgggtg cccggcacga
      961 tcacctggac cgcgtttggc agggcccggg tcagggcttc gggatcgccg ttgtcccggt
     1021 cttggtcgcc gttgacgatc agagtcggca tggccagcgc ggccagcgct ttgggcgggg
     1081 tatccgcgaa ggtctggagc agcagacggg tggcgacacg gtccaccttc atcgtcttca
     1141 tgaagctgac cgcgggaaaa atcggatcgc cgggcttcgc ttcatcaaag cggtcgatcg
     1201 cgtcgatgaa gaaagcgccg cgcccggtcc agccggccag cccttccagc cccatcccgc
     1261 ccagcgccag ccgcgccggc cgcaagcccc cgatcacccc gcgtaccgat gtccgcgcgc
     1321 cgagcgagaa accgcacagg tcatagtcct ccagccccag gtgcgcgacc agcgcggcca
     1381 ggtccttggc gaggacatca tccggatagg cggcgggatc gtgcggcgcg gcgctgtggc
     1441 cgtgggcgcg cagatcgggc atcaggcatt caaagccggc ctcggccaag agagcggcat
     1501 ggccccactt tatccagttc atctcggcgg tggagaacag cccgtggagc aggatcagcg
     1561 gccggccgct gccgctggtg ggggccatgc ggtgcagcgc cagctgcgcg ccgtcaaagc
     1621 cggggtagaa ttcggtttcc atgcccccgc cctagcgcaa gtgcagacgc cagcgccaga
     1681 ccgtcccttc atcggggtcc tgcgcttcgc catcgcgcgc gaaaccgttg cgccggagga
     1741 ctccggtcga ggcattctcc tgcggcaggg tgtgcgcaat caccgcggcc agcccctgcg
     1801 cgcgggcgat cgcgatcatc tccgcggcga ccgcgctggc gacgccgcgc ccttcgtgcg
     1861 ccgggaaagt cagatagccg atctcgatca cgccatcccc atccggttcg cccacaaatc
     1921 cgccgaagcc caagggcagg ttaccctgcc agccgatgta gccgcaccag ggcggcacct
     1981 gcgcctgtgc ggacagcgcc gccatctgct ccgcgaatgc tcccgtcacc gccggatcgc
     2041 tcagttccgc ttcaccggcg caggggcgaa gctccagctt cattccggca cttccagccc
     2101 ggcgtcctgc cactgcttca ccacgctggg cgtgctggcg ctatagaagc gcggcaggtc
     2161 ccgggtatcg agccaggcat cgtgcatcga ttcgcagccg gcatgggcag ccgggcgaaa
     2221 ccgcgtggga tcgtcaaagc tgccgacggt caggtccatc tcgccgctgc cgttggccca
     2281 ggcaaagccc agcggggttc cgcatttcga acagaacggc cgccgcgcaa cgggagagct
     2341 ttcgtaccag tccggctcgg tcagccaggt gaccttggcc ggctccacct gcacaaaggc
     2401 cgccgcgatc ccgccggtcg ccttctggca cttgcggcag tggcaccaat aggcctcgtc
     2461 gccgtcaatc tcggcggaat agcgaacacg gccgcactgg cagccgccgg tcatggtctg
     2521 ggtcatggga tcaggttagc gggttgccgg gtcatttcca tccccttgca gcaggcggcg
     2581 cacggggatc agtgccaaaa ttgcgccgaa ggccgcgatc gccatgtcct tctgggcgtc
     2641 gaaacggtcg ccctgttcgc cgttgtaggc cccggcatcc tccggcgcga gggccagggt
     2701 cagcgtccat tcgaagatct cataaagccc gccgacggcc aggacgaagg ccatggcgcc
     2761 ccacagcgcc gcccgcaggc tcagcccacc gtggcgcgtc gcaatctcgg caaagggcag
     2821 tacggcgagg acgccgaaca ggaaatggac cagccgatcg aacatgttgc gttcgaaccc
     2881 gaacgcggac cccaggcctg gcaaccagtg gtcatagggc acaaagctgt aggaccaacg
     2941 cgcggcgaac aggtgcagca ggacgaaggc ggcaaggcag cccgccgccg catcggaaat
     3001 cggatagcgc cgcatagccc accaggccgc aggcagcagc agcgcgaccg ggccaacctg
     3061 cagccaggtg ttttcgggat agcgcgcgcc aaatgcgctg agcgggatcg ccagggcgat
     3121 tagcgcgaag aggcggcgtt ggatcaaggg ccaagggtgc tcaaaccagg gcggacttgc
     3181 aagccagcgc tgccccgcta ttttcgcgtg gctctcagcc aagccggcag acttgacttg
     3241 gcgaggggat cagctggggc ttcga
//
LOCUS       DASUDV010000138         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_688769,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000138 DASUDV010000000
VERSION     DASUDV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688769"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1265)
                     /locus_tag="VFV30_03030"
     CDS             complement(114..1265)
                     /locus_tag="VFV30_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847093.1"
                     /translation="MTGRTRRGQPLVALGLILASWVGVRAAVWEAPRFEAAPAKPALV
                     AAPALPGSLPVTIAEPGADKIDPVATDWPVLMARPDAGPAPIDVPRAEPARPHLIAPP
                     PVEAVPAAQPLPPRIAAGHQLLWLAGVSQLPLPPELAQRLAAPAASPPAKASPVRATS
                     RWSADGWLLLRPGGNGFNLPGAGLPGANLPSGAYGASQAGAVLRYRLAPDSRHRPALY
                     LRASSALQRPRGEEAALGLSLRPIPTLPVAAMGEVRVTRTLSGNVVRPAAALVTELPP
                     ASLPLGLRGEAYGQAGWVGGKGHTLFVDGQARLERPIVNLGKFELRAGAGAWGGAQKG
                     ASRLDLGPTATLGVPLGPVNGRVSADWRFRVSGNAAPGSGPALTLSAGF"
     gene            complement(1262..2689)
                     /locus_tag="VFV30_03035"
     CDS             complement(1262..2689)
                     /locus_tag="VFV30_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEX4847094.1"
                     /translation="MIEPGSALDTALQWLLVSERELLVFAAFWFCVGLVDEFALDCLW
                     LWLKLTGRAKTRRLRPGHGTGPLASPAAVLIPAFQESTVIGATVAYMLRVWPQQELRI
                     YVGCYANDPATLAAASQAAGSDPRVRLVVHGARGPTTKADCLNRLYLALRQDEARGAL
                     RFGSVILHDAEDMVHPAALSVIDTELTRCDFVQLPVRAELQPSSRWIGAHYADEFAES
                     HAKAMVVRDAMGAALPAAGVGCGFSRTALARLEERRANEGLSGPFAPESLTEDYELGL
                     MLSREGRGSRFLRLRDSAGQLVATRSYFPADLASAVRQKTRWVHGIAFQGWERMGWSA
                     RPIELWMALRDRRGPLTALVLAAAYLLLAVDAVLLAAHLAGWRGDLTLSPALRLMLML
                     SLAGFVWRAGLRGLFTAREYGWREGVRGILRIPVGNIIAIMAGRRALAAYMRSLRGEA
                     VVWDKTVHQHHPAAIVPHQLEEVRA"
BASE COUNT          480 a          992 c          921 g          369 t
ORIGIN      
        1 cccagcgcga cgatgaccag gccattgacc gaaaggttgg cgatcgggag atagacgtcc
       61 atcttgccct gccggactaa cccagcaagg cgcgcgatga aagcctcagg tgcctagaaa
      121 cctgcggaaa gcgtcagcgc cggacccgat ccgggggcgg cattgccgct gacccggaac
      181 cgccagtctg ccgagacccg gccattgacc ggtccgagcg gcacgcccag cgtcgcggtc
      241 gggccaagat cgagccggct ggcccccttc tgcgctccgc cccaggcccc tgccccggcg
      301 cgcagctcga acttgccaag gttgacgatt ggcctttcga gccgcgcctg gccatcgacg
      361 aacagggtgt gacccttgcc gccaacccag ccggcctggc cataggcctc gccgcgcagc
      421 cccagcggca ggcttgccgg gggcaactcg gtgaccagcg cggcggccgg gcggaccaca
      481 ttgccgctga gcgtgcgcgt gacccgcact tcgcccatcg cggcaactgg cagcgtcggt
      541 atcggccgca gcgaaaggcc aagggcagcc tcctcgccgc gcgggcgctg cagcgcggaa
      601 ctggcccgca gatacagcgc cgggcgatgg cgactgtccg gggcgaggcg gtagcgcaac
      661 accgcacctg cctggcttgc tccataggca cccgaaggca ggtttgcgcc cggcaagcct
      721 gctccgggca ggttgaagcc attgccgccg gggcgcagca acagccagcc atcggccgac
      781 cagcggctgg tggcccgcac cggcgaggcc ttggcaggtg gcgaggcggc cggggccgcc
      841 agccgctggg ccaactcggg cggaagcgga agctgcgaga caccggccag ccacagcaac
      901 tggtgccccg cggcaatccg cggcggcagc ggctgtgcgg caggaaccgc ttcaacgggc
      961 ggcggcgcga tcagatgcgg tcgtgccggt tcagcgcgtg gaacgtcaat cggcgcggga
     1021 ccggcatcgg gccgggccat cagaaccggc cagtcggtcg cgacaggatc gatcttgtcc
     1081 gcaccgggtt cagcgatggt gaccggcagc gatcctggca gggccggtgc ggcaaccagc
     1141 gccggctttg caggagccgc ctcgaacctt ggcgcttccc agactgcggc acgcacgccg
     1201 acccagctcg cgaggatcag gcccagcgcg acaagcggct ggccacggcg ggtgcgcccg
     1261 gtcatgcccg cacctcttca agctggtgcg gcacgatcgc cgctggatgg tgctggtgca
     1321 ctgtcttgtc ccagaccacg gcctcgcccc gcagcgagcg catataggcg gccagcgccc
     1381 gcctgccggc catgatcgcg atgatgttgc ccaccgggat ccgcaggatg ccgcgcaccc
     1441 cttcgcgcca gccatattcg cgcgcagtga acaggccgcg cagccccgcg cgccagacaa
     1501 agccggccag gctgagcatc agcatcagcc gcagcgcggg ggaaagcgtc agatcgcccc
     1561 gccagcccgc caggtgcgca gccagcagca cggcatcgac cgccagcagc agataggccg
     1621 cagccaggac cagcgcggtc agcggcccac gccggtcgcg cagcgccatc cacaattcga
     1681 tcggccgcgc gctccagccc atccgctccc acccctggaa ggcgatgccg tggacccagc
     1741 gcgtcttctg gcgcaccgcg ctggcaagat cggccgggaa atagctgcgt gttgccacca
     1801 gctggcccgc actgtcgcgc agccgcagga accggctgcc gcgcccctcg cgcgagagca
     1861 tgaggcccag ttcatagtcc tcggtcagcg attcgggggc aaagggcccg ctcaggccct
     1921 cgttcgcgcg gcgctcttct agccgcgcca gcgcagtgcg cgagaagccg caaccgacgc
     1981 ccgccgcggg caatgccgcg cccatcgcat cgcgcacgac catcgccttg gcatggcttt
     2041 cggcaaattc atcggcatag tgcgccccga tccagcgcga cgacggctga agttcggccc
     2101 gtaccggaag ctggacgaaa tcgcagcggg tcagctcggt atcgatcacg ctcagcgccg
     2161 ccgggtggac catgtcttcg gcgtcgtgca ggatcacgct gccaaagcgc aacgcgccgc
     2221 gcgcctcgtc ctggcgcagg gccaggtaaa gacggttgag gcaatcggcc ttggtggtgg
     2281 gcccacgcgc gccatgcacc accagcctta ccctgggatc gctgcccgct gcttggctgg
     2341 cagcggcaag cgttgccgga tcgttggcat agcagccaac gtagatccgc agctcctgct
     2401 gcggccagac cctgagcatg taggcaacgg ttgcgccgat caccgtgctt tcttgaaagg
     2461 ccgggatcag caccgcggca ggcgatgcca gcggaccggt accatgaccc gggcgtaacc
     2521 ggcgggtctt ggcccggccg gtcagcttga gccacagcca cagacaatcg agcgcgaatt
     2581 cgtcaaccag tccgacgcaa aaccagaacg cggcgaagac aagcagttcg cgttccgaaa
     2641 ccagcaacca ttgcagggca gtatccagcg ccgatcctgg ctcgatcata tcgattttgc
     2701 cccgctagca gtgttgcgac aagccagcct atcggcatta ctacgtggtt gcaacgcggc
     2761 tt
//
LOCUS       DASUDV010000139         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1815617,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000139 DASUDV010000000
VERSION     DASUDV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815617"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /gene="typA"
                     /locus_tag="VFV30_03040"
     CDS             complement(<1..1722)
                     /gene="typA"
                     /locus_tag="VFV30_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX4847095.1"
                     /translation="MSAPLPLRNIAIIAHVDHGKTTLVDQLFRQSGTFRDNQRVEERA
                     MDSNDLEKERGITILAKCTSVEWNGTRINIVDTPGHADFGGEVERILSMVDGVILLVD
                     SSEGAMPQTKFVTGKALALGLRPIVVVNKIDRPDERIQEVLDEVFDLFVSLDATDEQC
                     DFPVLYASGRNGYANEDPSLREGTLTPLFEKIVDHVPPPAADVDGEFKFLVTLLDRDN
                     FLGRILTGKVQSGTVKVNSPIHALDNEGKVIETGRASKLMAFRGLERVPVEEARAGDI
                     ISIAGLTEATVANTICDPSVTEPLHAQPIDPPTLSMRFAVNDSPMAGREGTKVTSRMI
                     RDRLEREAESNVAIKVTESADKDSFEVAGRGELQLGVLIETMRREGFELGISRPRVLY
                     RQDEAGNRTEPYETVVIDVDDEHSGTVIDKMAIRKAEMLDMRPSGGGKTRITFSAPSR
                     GLIGYHGEFLSDTRGTGIMNRLFEKYGPYKGPIEGRPNGVLISNCAGEAVGYALNSLE
                     DRGVLFVRPQDKIYEGMIIGENAKPDDLEVNPMKSKQLTNFRSTGKDDAIRLTPPRIM
                     TLEQAIAY"
     gene            1901..2392
                     /locus_tag="VFV30_03045"
     CDS             1901..2392
                     /locus_tag="VFV30_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX4847096.1"
                     /translation="MLDWMLMPLRRYADFNGRSRRKEFWSFFLLNMIVSAVLIGPLYS
                     VIISNAMTMAASDPDSFEAAAASGFQFGDHPIATALAGLGILWSLGTLIPNIAVTVRR
                     LHDRDMSGWWYLGMIVASIIPFLGILAALAFLVLMFLEGTKGPNRFGPDPKAPAGNSD
                     VFN"
     gene            2506..2985
                     /locus_tag="VFV30_03050"
     CDS             2506..2985
                     /locus_tag="VFV30_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4847097.1"
                     /translation="MPVYGGFWIRFVAYIIDAVILFFAEAVIGAALGLTSGFAQTVSL
                     FTGADIATETDLLASGISIVMGWLYFALMESSGSQATVGKLALGLVVTDTAGDRISFL
                     RATGRHFAKFLSSLILLIGYIMIAFTDRKQGLHDMIASTLVTKKGTLQGVTSADVFR"
     gene            complement(2986..>4610)
                     /locus_tag="VFV30_03055"
     CDS             complement(2986..>4610)
                     /locus_tag="VFV30_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4847098.1"
                     /translation="IARIYLLQVAAAAVAGSLLGLAAGVLVTPLLAKALGELLPVAAG
                     FTFDPWALLRAAAFGLLVALVFAAPPLLSARRYPAMALFRARVSPLARQWRDAALPVG
                     LGLAGIAALALIGARQPAMVAGFLGGAAAILLLLGALGWAIRATAARLPRPQRPLLRI
                     ALSNLHRPGAQTGALVTALGFGLAAFVLLAAIQTSLDANIQARVPQRAPDYFVLDVPR
                     DRAGDFVKTVETQAPGARVRTVPALRGAILAYGAEGRMIRVATLKRIPEDAWPLRGER
                     GLTYSEEVPEGNVVTEGKWWPANYAGEPLVSIDERLAEALDLKIGDQLTIGLLGVERT
                     ARISSFRRIDWDSLGFNYVLVFSPNAIRDAPHNYAATIDLPKAGDRGAMLRTLVRQFP
                     TSSVIEVGGVLNQARDLLRQVSLAVLSAASVAVLAGIAVLLGAIAAARSSRLYDNVIL
                     RVLGASRRQLLGLQLAEYGLLSLVLAGVALALGTGTAWLVVVQLFEFEWLPDWPVVLA
                     TLGMGLALVIAFALAASLPLLRAKPAQALREL"
BASE COUNT          721 a         1532 c         1567 g          790 t
ORIGIN      
        1 gtaggcgatc gcctgttcca gcgtcatgat ccgcggcggg gtcaggcgga ttgcgtcgtc
       61 cttgccggtc gagcggaagt tggtcagctg cttcgacttc atcgggttga cttcaagatc
      121 gtccggcttg gcgttctcgc caatgatcat gccttcatag atcttgtcct gcgggcgcac
      181 gaacagcacg ccgcggtctt cgaggctgtt gagcgcatag cccaccgctt cgcccgcgca
      241 gttcgagatc agcacgccgt tggggcggcc ctcgatcggg cccttgtagg gaccgtactt
      301 ttcgaacagc cggttcatga tcccagtgcc gcgcgtgtcg gacaggaatt cgccgtgata
      361 gccgatcagg ccgcggctgg gggccgagaa ggtgatgcgg gtcttgccgc cgcccgaggg
      421 gcgcatgtcg agcatctcgg ccttgcggat cgccatcttg tcgatcaccg tgcccgagtg
      481 ttcgtcgtcc acgtcgatca ccacggtttc atacggctcg gtgcggttgc cggcctcgtc
      541 ctggcggtac agcacgcgcg ggcggctgat gcccagctca aagccttcgc ggcgcatcgt
      601 ttcgatcagc acgcccagct gcagttcgcc gcgcccggcc acttcgaagc tgtccttgtc
      661 ggcgctttcg gtgaccttga tcgccacgtt ggattcggct tcgcgctcca gccggtcgcg
      721 gatcatgcgg ctggtcacct tggtgccttc gcggccggcc atcgggctgt cgttcaccgc
      781 aaagcgcatc gacagggtcg gcggatcgat cggctgggcg tggagcggtt cggtgaccga
      841 ggggtcacag atcgtgttgg ccacggtcgc ctcggtcagg ccggcgatcg agatgatgtc
      901 gcccgcgcgg gcctcttcca ccggcacgcg ttccagcccg cggaaggcca tcagcttgct
      961 ggcgcggccg gtttcgatga ccttgccttc gttgtccagc gcgtggatcg gcgaattgac
     1021 cttgaccgtg cccgactgga ccttgccggt caggatgcgg cccaggaagt tgtcgcgatc
     1081 gagcagggtg accaggaact tgaattcccc atcgacatcg gccgccgggg gcgggacgtg
     1141 gtcgacgatc ttttcgaaca gcggcgtcag cgtgccttcg cgcaggctcg ggtcctcgtt
     1201 ggcatagccg ttgcggcccg aggcgtagag caccgggaaa tcacactgct cgtcggtcgc
     1261 gtcgaggctg acgaacaggt cgaacacttc atccagcact tcctggatgc gctcgtccgg
     1321 gcggtcgatc ttgttgacca ccacgatcgg gcgcaggccc agcgccagcg ccttgccggt
     1381 gacgaacttg gtctgcggca tggcgccttc cgagctgtcg accagcagga tcacgccgtc
     1441 gaccatgctc aggatccgct ccacctcgcc gccgaagtcg gcgtggccgg gggtgtcgac
     1501 gatgttgatc cgggtgccgt tccattcgac cgaggtgcac ttggccagaa tggtgatccc
     1561 gcgctccttt tcgaggtcat tggaatccat cgcgcgctct tcgacgcgct ggttgtcgcg
     1621 gaacgtgccg gactggcgga acagctggtc gaccagcgtg gtcttgccgt ggtcgacgtg
     1681 ggcgatgatc gcgatattgc gcagcggcag cggggcggac atgggtttct ctcgaagtca
     1741 gggaggaatg ggtgcgtgtt gcaccgcaac attgcgcgcg cccctagcgg atgtgagggg
     1801 ctagggcaag cgcgctggcg gctggcgcgg ttgccaaagc gggcaaagtg tggtgctctt
     1861 ccccccttgg cgccgcaacg gcgcagggaa gggggttact atgcttgatt ggatgctgat
     1921 gccgctgcgg cgctatgccg acttcaacgg gcgctcgcgc cgcaaggaat tctggtcttt
     1981 cttcttgctc aacatgatcg tcagcgcggt cctgatcggg ccgctctatt cggtgatcat
     2041 cagcaatgcc atgaccatgg ccgcgtccga tccggacagc ttcgaagccg cggccgcatc
     2101 cgggttccag ttcggcgatc atcccatcgc cacggcgctg gcgggcctgg gcatcctgtg
     2161 gtcgctcggc acgctgatcc cgaacatcgc cgtcaccgtt cgccgcctgc atgaccgcga
     2221 catgagcggc tggtggtacc tgggcatgat cgtggccagc atcattccgt tcctcggcat
     2281 tctggcggcc ctggccttcc tggtgctgat gttcctggaa ggaaccaagg ggccgaaccg
     2341 gttcgggccc gatcccaagg cgccggccgg gaattccgac gtcttcaact gattttcaac
     2401 ggcaattcga gccgctgcgg gtccgggccc gcggcggctt ttgcttgcct gccgggccaa
     2461 gctgtggtcc ttttggagcc tcggtcgtga acaggggagg tgggcatgcc ggtatacggg
     2521 gggttctgga tccggttcgt ggcctacatc atcgatgcgg tcatcctgtt ctttgccgaa
     2581 gcggtgatcg gcgctgcgct gggcctcact tcgggctttg cccagacggt cagcctgttc
     2641 accggtgccg atatcgcgac cgaaaccgat ctgctcgcct ccgggatcag cattgtgatg
     2701 ggctggctct actttgccct gatggaaagc tcgggctcgc aggccaccgt cggcaagctg
     2761 gcgctggggc tggtcgtgac cgacacggcg ggtgaccgga tcagcttcct gcgcgccacc
     2821 gggcggcact ttgccaagtt cctgtccagc ctgatcctgc tgatcggcta catcatgatc
     2881 gcctttaccg accgcaagca aggcctgcac gacatgatcg ccagcacgct ggtgaccaag
     2941 aagggcacct tgcagggcgt tacctcggcc gacgtgttcc gctgattaga gctcccgcag
     3001 cgcctgcgcc ggcttggccc gcagcagcgg gagcgaggcg gccagcgcga aggcgatgac
     3061 cagcgccagg cccatgccca gcgtggccag caccaccggc cagtcgggca gccattcgaa
     3121 ttcgaacagc tggaccacca ccagccaggc cgttcccgtg ccgagcgcca gtgccacccc
     3181 ggccagaacc agcgagagca ggccgtattc ggccagttgc aggcccagca gctggcggcg
     3241 gctggcgccc agcacgcgca ggatcacgtt gtcgtaaagc cggctggaac gggccgcggc
     3301 gatcgcgccc agcagcacgg cgatcccggc cagcaccgcc accgacgcgg cggacagcac
     3361 cgccaggctg acttggcgca acaggtcgcg cgcctggttc agcacgccgc cgacctcgat
     3421 caccgaactg gtcgggaact ggcgcaccag ggtgcgcagc atcgccccgc ggtcacccgc
     3481 cttgggcagg tcgatcgtcg cggcgtaatt gtgcggggca tcgcggatcg cgttggggct
     3541 gaacaccagc acatagttga agcccagcga atcccagtcg atccgccgga acgaggaaat
     3601 ccgcgcggtc cgctccacgc ccaacaggcc gatggtcaac tggtcgccga tcttcaggtc
     3661 cagcgcctcg gccagccgct cgtcgatcga aaccagcggc tctccggcat aattcgcggg
     3721 ccaccacttg ccttcggtca ccacgttgcc ttcgggaacc tcttcggaat aggtcagccc
     3781 gcgctcgccg cgcagcggcc aggcgtcttc ggggatgcgc ttcagggtgg ccacgcggat
     3841 catccggcct tccgcgccat aggccaggat cgcgccgcgc agcgccggga cagtgcggac
     3901 ccgcgcgccg ggggcctggg tctccaccgt cttgacgaag tccccggccc ggtcgcgcgg
     3961 tacgtcgagc acgaagtaat cgggtgcgcg ctgcggcacg cgggcctgga tgttggcgtc
     4021 cagactggtc tggatcgcgg cgaggaggac aaaggcggcc aggccaaagc ccagcgcggt
     4081 gaccagcgcg ccggtctggg cgccggggcg gtgcaggttg gagagggcaa tgcgcagcag
     4141 cgggcgctgc ggacggggca ggcgcgcagc ggtggcgcga atggcccagc cgagcgcgcc
     4201 cagcagcagc aggatcgccg ccgcgccgcc caggaacccg gcaaccatgg ccggctggcg
     4261 cgcgccgatc agggccagcg cggcaatccc ggccagcccc agcccgaccg gcagcgccgc
     4321 atcgcgccat tgccgggcca gcggagagac ccgcgcccgg aacagcgcca tcgccggata
     4381 gcgccgggcc gagagcagcg gcggtgcggc aaagaccagc gcgaccagca ggccaaaggc
     4441 cgcggcgcgc agcagcgccc agggatcgaa ggtaaagccg gccgcaaccg gcagcagttc
     4501 gcccagcgcc ttggccagca gcggcgtgac cagcaccccg gcggccaggc ccagcagcga
     4561 tccggcgacc gccgccgcgg caacctgaag cagataaatc cgcgcaatat
//
LOCUS       DASUDV010000140         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_357761,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000140 DASUDV010000000
VERSION     DASUDV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357761"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFV30_03060"
     CDS             complement(<1..463)
                     /locus_tag="VFV30_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4847099.1"
                     /translation="MVRRLAPLLALVLGACAPGAAKEAGPGLPTLVSLNPCTDAVLTE
                     VADPQQILAISAYSHDPASSSMDLNTARKFPAVSGTVEEVLALHPDVVIGTPFMSPAT
                     TQAFDRLGLRLEQVGIATTVEDSLAQVRQIAALAGHPDRGEALVDRIEAALA"
     gene            complement(457..2349)
                     /locus_tag="VFV30_03065"
     CDS             complement(457..2349)
                     /locus_tag="VFV30_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847100.1"
                     /translation="MKYLFLLTASLTVATPALAQGQKPLQAAEARCNPETDENCPHVP
                     MLKEAPDDQITVTATGLWARRVQNGQSISVLTAEDLRSIQGADPVRVIERMPGVSLAR
                     SGPLGSQTALFVRGANSEQLLVMIDGIRVTDQASPSGGFDLGTLTTGGIGRIELLRGS
                     NSLAWGSEAIGGVLAVTSDERTGLRGSFEYGAHDTLDAQLGGGLSGDNYGLGLSGGYT
                     RSDGISAYAPGTEADGFRQWRIGGRGHLDLSDTVKIVAAGRYADSKIDFDGFPPPAYS
                     FTDTPEYQTTRQGSGRLGLEYASTGLQLSGGVALSDTRRAYFDPTYGSAPNFETEGRS
                     VHADLKGRTDLTDAVTLDFGADGDWSRFSTTFDSRQTARTIGGHALLRVRTGQLNLSG
                     GVRIDDHDRFGTQWTFGANGSLGLTTGLRLRASYGQGFKAPTLSQLFGFGGNTALQPE
                     ESEAFDVGLEYATRDGSLKVGATLFRRDSTNLIDYVWPTGYFNVGRARAQGLELEGVA
                     KVTPRFTVRAAYTWLEATNRITGTDLARRPRHTLSASADWTTPLGGLALGADVRLADD
                     SFDDRGNFTRLDGYALVTVRASLPLGERVELFGRIENLADVQYQTVFGYGTYGRSGYA
                     GIRVKW"
     regulatory      complement(2443..2644)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          401 a          922 c          918 g          413 t
ORIGIN      
        1 cggccagcgc cgcctcgatc cggtccacca gcgcttcgcc gcggtcggga tggccggcca
       61 gggcggcgat ctgccggacc tgggccagac tgtcctccac cgtggtggca atgccgacct
      121 gttccagccg cagccccagg cggtcaaagg cctgggtggt agccgggctc atgaacgggg
      181 tgccgatcac cacgtccggg tgcagcgcca acacctcctc gaccgtgccc gacaccgccg
      241 ggaacttccg cgcggtgttc aggtccatcg agctggaggc cggatcgtgg ctgtaggcgg
      301 aaatggccag gatctgctgc ggatcggcca cttcggtcag caccgcatcg gtgcaggggt
      361 tgaggctgac caacgtgggc agccccggcc ccgcctcttt cgccgcgccg ggggcgcagg
      421 cccccagcac gagcgccagc agcggggcca gccgccttac cacttcacgc ggatcccggc
      481 atagccggag cggccgtagg tgccgtagcc gaagaccgtc tggtactgga catcggcgag
      541 gttctcgatc cggccgaaca gctccacccg ctcgcccagc ggcaggctgg cgcggaccgt
      601 gaccagcgca tagccatcga gccgggtaaa gttgccgcgg tcgtcaaagc tgtcgtcggc
      661 cagacggaca tcggcgccca gcgccaggcc gccaagcggc gtggtccagt cggccgaggc
      721 cgacagggtg tggcgcggac ggcgggccag atccgtgccg gtgatccggt tggtcgcctc
      781 cagccaggta taggccgcgc ggacggtgaa gcgcggggtg accttggcca cgccttccag
      841 ctccagcccc tgggcgcggg cccggccgac gttgaaatag ccggtcggcc agacgtaatc
      901 gatcaggttg gtgctgtcgc ggcggaacag ggtggcgccc accttcagcg agccatcgcg
      961 ggtggcgtat tccagcccga cgtcaaaggc ctcgctctct tccggctgca gcgcggtgtt
     1021 gccgccaaag ccgaacagct gcgacagggt cggcgccttg aagccctggc catagctggc
     1081 ccgcagccgc agcccggtgg tcagccccag gctgccgttg gcgccaaagg tccactgggt
     1141 gccgaaccgg tcgtgatcgt cgatccggac cccgcccgag aggttgagct gcccggtgcg
     1201 cacgcgcagc agggcgtgcc cgccaatcgt ccgcgcggtc tggcgcgaat cgaaggtggt
     1261 cgagaaccgc gaccagtccc cgtccgcccc gaaatcgagc gtgacggcgt cggtcagatc
     1321 ggtgcggccc ttgagatcgg cgtggaccga acggccttcc gtttcaaagt tcggggccga
     1381 gccataggtc gggtcgaaat aggcccggcg ggtatcggac agcgccaccc cgccgctgag
     1441 ctgcaggccg gtgctggcgt attccagccc gagccggccc gaaccctgcc tggtggtctg
     1501 gtactcgggc gtatcggtga agctgtaggc cggcggggga aacccgtcga aatcgatctt
     1561 gctgtcggca tagcggccgg cggcgacgat cttcacggtg tcgctgaggt ccagatgccc
     1621 gcgcccgccg atccgccact ggcgaaagcc gtcagcctcg gtgccggggg cataggccga
     1681 gatcccgtcg ctgcgggtgt agccgccgct cagccccagg ccgtaattgt cccccgacag
     1741 gccgccgccc agctgcgcgt cgagcgtatc gtgggcgccg tattcgaagg agccgcgcag
     1801 gccggtgcgt tcatcgctgg tcacggccag cacgcctccg atggcctcgc tgccccaggc
     1861 caggctgttg gagccgcgca gcagctcgat ccgtccgatc ccgcctgtgg tcagcgtgcc
     1921 gagatcgaac ccgcccgagg gcgaggcctg gtcggtcacc cggatgccgt cgatcatcac
     1981 cagcagctgt tcggaattgg ccccgcgcac gaacagcgcg gtctggctgc cgagcgggcc
     2041 gctgcgggcc agcgagacgc cgggcatccg ctcgatcacg cggaccggat cggcgccctg
     2101 gatggagcgc aggtcttcgg cggtgaggac cgagatcgat tggccgttct gcacccgccg
     2161 tgcccacagg ccggtggcgg tgacggtgat ctgatcgtcc ggcgcttcct tcagcatcgg
     2221 aacgtgcgga cagttttcat ccgtttccgg attgcagcgg gcttcggccg cctgcagcgg
     2281 cttttgcccc tgggccagcg ccggggtggc cacagtcagc gaagcagtca aaagaaacag
     2341 gtatttcaca cgcaatactc ccgtcatgaa cgttgccgtc catggcgaaa gcgcccgcac
     2401 cattgcgggc cttgcgctgc attccggtac accccgcccg gctgcggaac gaccgtcatg
     2461 ggcaggtcta ttggctcccg gatcaatggt gttgccccgc cttcccggtg ggcccttgca
     2521 gacccgctcc agtggcattg tggggcaacc cctccccggt cacagttgcg ggggcagcgg
     2581 gggaattgaa cccccttccc tcttagaccc ggtttcccgg gtaacccgtg acgagccccg
     2641 cccatagccg gggc
//
LOCUS       DASUDV010000141         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1550694,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000141 DASUDV010000000
VERSION     DASUDV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550694"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFV30_03070"
     CDS             complement(<1..602)
                     /locus_tag="VFV30_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4847101.1"
                     /translation="MWDRIAAMIFKEVRQMARDPGTVAMMLMFPIIQLLIFGVAINND
                     PRHLPLAVEANDHSAFSRAIVSALRQTSYFDVVELPAGAGEGDRLIAEGRAQFVLTIP
                     ADFSRDLVRGHRPDLLLSVDATDPASTGPAVAAVSEAIESAIARELTGALAIRRPTPS
                     PVNMVLHRSYNPEGITSHNTVPGLLAIILSMTMVALTAMSV"
     gene            complement(609..1553)
                     /gene="ccmA"
                     /locus_tag="VFV30_03075"
     CDS             complement(609..1553)
                     /gene="ccmA"
                     /locus_tag="VFV30_03075"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX4847102.1"
                     /translation="MNAAAPDPVPVIAVRGLTKRFGARTVVDHVDLAVNQGRICGFLG
                     PNGSGKTTTLRMICGLLIPDEGEGEVLGLDLRTQRRAIKQQIGYMTQKFGLFSDLSIA
                     ENLEFMARVHGLDRRKARVDEALERLGLASRSTQLAGKLSGGWKQRLALAAAVLHEPK
                     ILLLDEPTAGVDPKARREFWDQIHALAQNGMTVLVSTHYMDEAERCHEIAYIAYGVML
                     ARGTIGEVIAGSGLKALQGEGPGADRLSAEIERQPGVAMAAPFGTSLHVCGPDLAALR
                     QAVRPWADRIAFTETDPTLEDVFIHLMKGAADNSVVEG"
     gene            complement(1550..2524)
                     /locus_tag="VFV30_03080"
     CDS             complement(1550..2524)
                     /locus_tag="VFV30_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX4847103.1"
                     /translation="MTALPKPLIAVVVLALAGGAGWYFTRPATPDHWLGYVEAETIYV
                     AAPVSGRLAERPVERGASVAAGTMLFALDPETTDAETARAQAQVVAAEAEASDLSSAR
                     QRQAELDISRAAEAGAAAQLTKAAQDYNRIAALAAKGFASRAQFDAARAARDAASAQL
                     AQTRAQIAAGQLSAGRAAQQAAARAQVAGAEAALRGQRQRRREIAPVSPSSGVVEQTF
                     YNPGEWVPANSPVVAVLPDDRRKLRFYVPEDRIAVLQPGATIRYSCDACGGERSAKIS
                     FISPRAEFTPPVIYSQHARAKLVFLVEALLPASDRPLPPGLPVEVAAQ"
     gene            complement(2521..>2958)
                     /locus_tag="VFV30_03085"
     CDS             complement(2521..>2958)
                     /locus_tag="VFV30_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX4847104.1"
                     /translation="TELIEAMLPEAAPAEFGDLPAEALLRHFIAVLAQRITSDDMAFV
                     PRVIIGEGMNFPDLARFYHDKVVARGLGIVTGIIEHGIARGELACASPPLAARSLISG
                     MLFAAIWKLVFVPVGAQAIDPGEFAQSHADTLLIGLRARKDHA"
BASE COUNT          493 a         1010 c          988 g          467 t
ORIGIN      
        1 accgacatcg cggtcagcgc caccatggtc atcgacagga tgatcgccaa aagaccaggc
       61 acggtgttgt ggctggtgat accttcggga ttgtagctgc ggtgcagcac catgttgacc
      121 ggcgatggcg tgggccggcg gatcgccaac gcgccggtca gctcgcgcgc aatcgcgctt
      181 tcgatcgcct cgctcactgc cgcaacggcc gggccggtgc tggccgggtc ggtggcatca
      241 acgctcagca gcaggtccgg ccggtgcccg cgcaccagat cgcgagaaaa atcggccggg
      301 atggtcagca cgaactgggc cctgccctcg gcgatcaatc ggtccccctc ccccgcaccg
      361 gcgggcagtt cgaccacgtc gaaataggag gtctgccgca gggcgctgac aatggcgcgc
      421 gaaaaggcgg aatgatcgtt tgcttcaacc gccagcggca agtgacgcgg atcgttgttg
      481 atcgcgacac cgaagatcag cagctggatg atcgggaaca tcagcatcat cgccaccgtt
      541 cccggatcgc gcgccatctg gcgcacttcc ttgaagatca tcgcggcgat ccggtcccac
      601 atcgcggctc agccctccac caccgaattg tcggcggcgc ccttcatcag gtggatgaac
      661 acgtcctcca gcgtggggtc ggtttcggta aaggcgatgc gatcggccca gggtcggacc
      721 gcctggcgca gcgcggccag gtctggcccg cacacgtgca gcgaagtgcc gaacggcgcg
      781 gccatggcca cgcctggctg gcgttcgatc tcggccgaaa gtcggtctgc acccggaccc
      841 tcgccttgca gcgccttcag tcccgatccg gcgatcactt cgccgatcgt gccgcgcgcc
      901 agcatcactc cataggcgat ataggcaatc tcgtggcagc gttcggcctc atccatgtag
      961 tgggtggaaa ccagcacggt catgccattc tgagccaggg catggatctg gtcccagaac
     1021 tcgcgccggg ccttgggatc gacccccgcg gtcggttcgt cgagcagcag gatcttcggc
     1081 tcgtgcagca ccgccgcggc cagtgccagg cgctgcttcc acccgcccga aagcttgccc
     1141 gcaagctggg tcgaccggct ggcaaggccc agccgctcca gcgcctcgtc gacccgcgcc
     1201 ttgcgccggt cgaggccgtg cacccgggcc atgaactcaa ggttttcggc gatcgacagg
     1261 tcggaaaaca gcccgaactt ctgcgtcatg tagccgatct gctgcttgat cgcgcggcgc
     1321 tgagtacgca ggtcaaggcc cagcacctcg ccttcgccct catccgggat cagcagtccg
     1381 cagatcatcc gcagcgtggt ggtcttgccc gagccgttcg ggccgaggaa cccgcagatc
     1441 cggccctggt tcaccgccag atcgacatgg tcgaccacgg tgcgcgcgcc gaaccgcttg
     1501 gtcaggccgc gaaccgcgat caccgggaca ggatcgggcg cggcggcgtt cactgtgcgg
     1561 ccacttccac cggcaggccc ggcggcagcg gcctgtccga agctggaagc aatgcctcga
     1621 ccagaaacac tagcttggcg cgggcatgct gcgaatagat caccggcggg gtaaattcgg
     1681 cgcgcggcga aatgaagctg atcttcgcgc tgcgctcgcc cccgcaggca tcgcagctgt
     1741 agcggatcgt tgcccccggc tggagcacgg cgatccggtc ttcaggcaca tagaagcgca
     1801 gcttacggcg atcgtcgggc agcaccgcca ccaccggact gttcgccgga acccattcgc
     1861 ccgggttgta gaaggtctgt tcgaccaccc cggacgaggg cgaaaccggg gcgatctcgc
     1921 gacggcgctg gcgctggccg cgcagcgccg cctctgctcc tgccacctgg gcgcgggcag
     1981 cggcctgctg cgcggcgcgg ccggcggaaa gctggccggc ggcaatctgg gcgcgggtct
     2041 gggcgagctg ggccgaagca gcatcgcgcg cggcccgcgc tgcatcgaac tgggcgcggc
     2101 tggcaaagcc tttggcggcc agtgcggcga tccggttata gtcctgcgcg gccttggtca
     2161 gctgggctgc cgcaccggcc tctgccgcgc gcgagatgtc gagttcggcc tggcgctggc
     2221 gggcgctgga aagatcgctg gcctcggcct cggccgccac aacctgggcc tgagcccgcg
     2281 cggtttcggc atcggtagtt tccggatcga gcgcgaacag catcgttcct gccgccacgc
     2341 tcgccccgcg ttcgaccggg cgttcggcca gccgacccga aaccggcgcg gcaacataga
     2401 tcgtctcggc ctcgacatag cccagccagt ggtcgggcgt tgccggacgg gtgaagtacc
     2461 agcccgcacc gccggcaagg gccagcacaa ccaccgcgat aaggggcttg ggcaaggcgg
     2521 tcatgcatgg tcctttctgg cgcgcaggcc gatcagcagt gtatcggcgt ggctttgcgc
     2581 gaattcaccc ggatcgatcg cctgagcgcc gacggggacg aacaccagct tccagatcgc
     2641 cgcaaacagc atgcccgaaa tcaggctgcg cgcggcaagc ggcgggctgg cgcaggccag
     2701 ttcaccgcgc gcgatgccgt gttcgataat gcccgtgaca atccccagcc cgcgcgccac
     2761 caccttgtcg tgatagaagc gcgcgaggtc gggaaagttc atcccctcgc cgatgatcac
     2821 ccggggcacg aaggccatgt cgtcgctggt gatgcgctga gccaggaccg cgatgaaatg
     2881 gcgcagcagg gcttcggccg gcagatcgcc gaattccgcc ggcgcggctt cgggcagcat
     2941 ggcttcgatc agttcggt
//
LOCUS       DASUDV010000142         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1771754,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000142 DASUDV010000000
VERSION     DASUDV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771754"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFV30_03090"
     CDS             <1..277
                     /locus_tag="VFV30_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847105.1"
                     /translation="PVYPRGHARQAAGSDSAACRIRVVNFVTPVSAEDVLGFYATQLT
                     RAGLAPALRREADDLVLRGQGKTLGFAIHARPFEAGLTEVVLVTRER"
     gene            complement(278..1693)
                     /gene="astD"
                     /locus_tag="VFV30_03095"
     CDS             complement(278..1693)
                     /gene="astD"
                     /locus_tag="VFV30_03095"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEX4847106.1"
                     /translation="MGTQQLVSYEPATGAELWRGKHGNVDDIVGRARRAWPAWAAQPL
                     ANRMELMRRFANEVRKENEKLATLIARETGKPLWEARTEVESVVNKVEISIRAYAERT
                     PQRKLDNALQGTAALRHKPHGVMAVLGPYNFPAHLPNGHIVPALIAGNAVVFKPSEKT
                     PATAELLVQCFHRAGVSAAVVQLLIGGPEEGQALVGHEGIDGVLFTGSAHTGIAINRR
                     LATRPDKIVALEMGGNNPIVAWDTPKLTDAAALIVQSAFTSAGQRCTAGRRLIVKSSM
                     YEALMREVKGLCDRIIVGAPFDEPSPFMGPVIDNMAADGLTESFLYLLSHGGKAIKHL
                     VRPRDDLPFLSPAIIDVTDVKDRPDVELFGPLLQVVQVKDFDEAIAEANATRFGLSAS
                     LIGGTPAEYNRFWANVRAGVINWNRPTNGASSAAPFGGVGLSGNHRPSAYYAADYCAY
                     PVASTEMEQPRAMIGVGLREA"
     gene            1869..2759
                     /locus_tag="VFV30_03100"
     CDS             1869..2759
                     /locus_tag="VFV30_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847107.1"
                     /translation="MHSQAAQEAGQTGAFADCFWTSRDGLRLHYRDYPGSGDRPPVLC
                     LPGLTRNARDFARLAERLSGQWRVLCPDLRGRGDSAYAKDWTSYNPLQYADDIALLFE
                     ELGLERAVFIGTSLGGLLTMMTALGAPERIAGACLNDIGPVIEPAGLERIRGYVGQGR
                     SFSTWMHAARAIRETQGPAFPDYDISDWLLAAKRCMVLGSNGRIHFDYDMNIAEPFAQ
                     PGGEAGVDLWAGLDALKGRPVLLLRGELSDLLSAATHRQMAKRLPDAEAVTVPRVGHA
                     PMLDEPEAVAAIDRLLARVK"
     gene            2756..3904
                     /locus_tag="VFV30_03105"
     CDS             2756..3904
                     /locus_tag="VFV30_03105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4847108.1"
                     /translation="MTASGRPLRLLHLHSSFAPGGKELRAAKLMNLFGAGIAHAVVSA
                     VPGELGAARAIDPAVAVTYPDRFPALAGSPTPWRLLTLARAMRGFDLVLTYNWGAMDA
                     VMAHRLFGALLRLPPLVHHEDGFNQDEAERLNPRRNAFRRIALARAAALVVPSRRLEA
                     IAREAWHQPAARVRRIANGIPLGSYGAPADPAAIAGLTKAPDEKWLGTLAGLRAVKNL
                     PRLVRAFAAMPEDWRLVILGEGPERAAIAAEAARLGLGERVHLPGFAADPARVLGLFD
                     LFALSSDSEQFPISVVEAMAAGLAVVSPRVGDVAAMLPDENQAWLTAPGDDSELGRAL
                     AGAAADPGARGRIGAANRALALTAYSEETMLAAYRDTYASALGRQTFP"
BASE COUNT          615 a         1301 c         1371 g          717 t
ORIGIN      
        1 gccggtctat ccgcgcggtc atgcccgcca ggccgcggga agcgacagcg ccgcctgccg
       61 catccgggtg gtgaacttcg tcaccccggt cagcgccgaa gatgtgctgg gcttctatgc
      121 aacgcaattg accagggccg gattggcccc ggccttgcgc cgcgaagccg atgatctggt
      181 gctgcgcggg cagggcaaga cgctgggctt tgcgatccac gcccggccgt tcgaagccgg
      241 gctgaccgaa gtggtgctgg ttacccgcga acgctaatca ggcttcgcgc aggccgacgc
      301 cgatcatcgc ccggggctgc tccatctcgg tcgaggcgac cggataggca cagtaatcgg
      361 ccgcgtaata ggcgctggga cggtggttgc ccgaaaggcc gaccccgccg aacggcgcgg
      421 ccgaactggc cccgttggtt ggccggttcc agttgatcac cccggcgcga acattggccc
      481 agaagcggtt gtattccgcc ggcgtgccgc cgatcagcga ggccgaaagc ccgaacctgg
      541 tggcgttggc ctcggcgatc gcctcgtcga aatccttgac ctggaccacc tgcaacagcg
      601 gcccgaacag ttcgacgtcg gggcggtcct tgacatcggt gacatcgatg atcgccgggg
      661 aaaggaacgg cagatcgtcg cgcgggcgca ccaggtgctt gatagccttg ccgccgtggc
      721 tgagcagata gaggaagctt tcggtcaggc cgtcggcggc catattgtcg atcactggcc
      781 ccatgaaggg cgaaggttcg tcaaacggcg cgccaacgat gatccggtcg cacagcccct
      841 tcacttcgcg catcagcgct tcgtacatcg atgacttcac gatcaggcgc cgacctgcgg
      901 tgcagcgttg gcccgcgctg gtgaaggccg actggacgat cagcgccgcc gcgtcggtca
      961 gtttgggggt gtcccaggcc acgatcgggt tgttgccgcc catttccagc gccacgatct
     1021 tgtcgggccg ggtggcaagg cggcggttga tcgcgatccc ggtgtgggcc gatccggtga
     1081 acaggacgcc atcgatacct tcgtggccga ccagcgcctg gccttcttcg ggcccgccga
     1141 tcagcagctg gacaaccgcg gccgaaaccc cggcgcggtg gaagcactgc accagcagtt
     1201 cggctgtggc cggggtcttt tccgatggtt tgaacaccac cgcattgccg gcaatcagcg
     1261 cgggaacgat atgtccgttg ggcaggtgtg cggggaaatt gtagggcccc agcacggcca
     1321 tcacgccatg cggcttgtgg cgcagcgccg cggttccctg cagcgcattg tcgagctttc
     1381 gctggggcgt gcgttcggca taggcgcgga tcgaaatctc gaccttgttg accacgcttt
     1441 caacctcggt gcgcgcttcc cacagcggct ttccggtttc gcgtgcgatc agcgtggcca
     1501 gcttttcgtt ttccttgcgc acttcgttgg cgaagcggcg catcagttcc atccggttgg
     1561 caagcggttg cgccgcccag gccggccagg cacggcgcgc ccgcccgacg atgtcatcga
     1621 cattgccgtg cttgccgcgc cacagttctg caccggttgc cggttcgtat gaaacgagtt
     1681 gctgcgtacc caaaattttg atcccgtccg ctttagccgc ttgtcgcata gcaggctggt
     1741 tcgccagagc ccttagccgg ttagtcgaat tgtgaaaagc cttggtttcc atccgattaa
     1801 tgcggttcaa ttttccgtcc gggccgggtt gcgcattggc tggcgctggg gcattgatcg
     1861 ggcctcaaat gcatagccaa gccgcccagg aagccggcca gaccggcgca ttcgccgact
     1921 gcttctggac cagtcgcgac ggcttgcggc tgcattaccg cgactatccc ggaagcggcg
     1981 atcgcccgcc agtgctgtgc ctgccggggc tgacccgcaa tgcccgcgat ttcgcacgtc
     2041 ttgccgaacg gctcagcggg cagtggcggg tgctgtgccc ggacctgcgc gggcggggcg
     2101 acagcgccta tgccaaggac tggaccagct acaatccgct gcaatatgcc gacgatatcg
     2161 cgctattgtt cgaggaactc gggctggaac gggcggtgtt catcggcacc tcgctgggcg
     2221 gcctgttgac gatgatgacc gcgcttggcg cgccagaacg gatcgcgggg gcctgcctca
     2281 acgatatcgg cccggtgatc gagcctgccg ggcttgaacg gatccgcggc tatgttggcc
     2341 aggggcgcag cttttccacc tggatgcacg ctgcccgggc gatccgcgaa acgcaaggcc
     2401 ccgcctttcc cgattacgat atctccgatt ggctgctggc ggccaagcgc tgcatggtgc
     2461 tgggcagcaa cggccggatc cacttcgatt acgacatgaa cattgccgag ccattcgcgc
     2521 agccaggggg cgaggccggg gtcgatctct gggctgggct cgatgcgctc aaggggcggc
     2581 cggtgctgct gctgcgcggc gaactgtccg atctgctttc ggccgcgacc catcggcaga
     2641 tggccaagcg cctgcccgat gccgaagccg tgacggtacc gcgggtaggc catgcgccga
     2701 tgctggacga acccgaagcg gttgccgcga tcgatcgatt gctggcccgc gtcaaatgac
     2761 ggcttcgggc aggccgctgc ggctgctgca tctccattcc agttttgccc cgggcggcaa
     2821 ggaactgcgt gcggccaagc tgatgaacct gttcggtgcg gggattgcgc atgccgtggt
     2881 ttcggccgtg ccgggcgaac tgggcgcggc gcgggcgatc gatccagccg ttgcggtaac
     2941 ctatccggac cgttttcccg cgctcgccgg tagccccacg ccctggcgcc tgctgaccct
     3001 ggcaagggcg atgcgcggct tcgatctggt gctgacctat aactgggggg cgatggatgc
     3061 ggtcatggcg caccgcctgt ttggcgcgct gctgcgcctg ccgccgctgg tccaccacga
     3121 ggacggcttc aaccaggatg aagccgagcg gctcaacccc cggcgcaacg ccttccggcg
     3181 gatcgcgctg gcccgggctg ccgcgttggt ggtgccttcg cgccggcttg aggcaattgc
     3241 ccgcgaagcc tggcaccagc cggccgcgcg ggtgcgccgg atcgccaacg ggatcccgct
     3301 tggaagctat ggcgcgcccg ccgatcctgc ggcgatcgcg gggctgacaa aggccccgga
     3361 cgaaaagtgg ctgggcacgc tggcgggcct gcgcgcggtc aagaacctgc cccggctggt
     3421 gcgggccttc gcggccatgc ccgaagactg gcgcctggtg atcctgggcg aggggcccga
     3481 gcgcgcggcg attgccgccg aagcggcgcg tctggggctg ggggagcggg tgcacctgcc
     3541 cggatttgcc gccgatcccg caagggtgct tggcctgttc gatctgttcg cgctgtcctc
     3601 cgacagcgaa cagtttccga tctcggtggt cgaggcgatg gccgccgggc ttgccgtggt
     3661 cagcccgcgg gtcggcgatg ttgcggccat gctgcctgat gagaaccagg cctggctgac
     3721 cgcgccaggc gatgattccg aactgggccg cgccctggcc ggtgcggcag ccgatcccgg
     3781 cgcgcgcgga cggatcggtg cagccaatcg cgcgctggcg ctgaccgcct attccgaaga
     3841 gacaatgctg gctgcctacc gcgataccta tgccagcgcg ctggggcggc aaactttccc
     3901 ctgattggcc gttcagcggg ccttcagcgg gggaaaggca cagatgaggc gttgaaaaga
     3961 cctgcccatt cgctaaacac ccgggccaat gcatcaacca gccc
//
LOCUS       DASUDV010000143         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1572865,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000143 DASUDV010000000
VERSION     DASUDV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572865"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFV30_03110"
     CDS             complement(<1..921)
                     /locus_tag="VFV30_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4847109.1"
                     /translation="MATLTNQSWLSRRGQYHPRLIEAAVAMNANDIPKAEFLLRTHLK
                     ERPFEVAAIRMLAEVAGRIGRYKDSETLLRRALELSPGFTAARANLALVLYRQNRPAD
                     AIAELNRVIAEEPENPGHANLQAAALGRIGEFEEAIPLYEKVLEKAPQQPRVWMSFGH
                     MLKTVGRLDEGIAAYRKAIELMPSLGEAWWSLANLKTVRFDDADIAAMEAALASGDVA
                     KDDRFHLDFALGKALEDRGETDAAFAHYAAANALRKTDLDYEADENERLVDRLIEVAT
                     PAYFAAREGQGCDAPDPIFVLGMPRAGSTLV"
     gene            992..2266
                     /locus_tag="VFV30_03115"
     CDS             992..2266
                     /locus_tag="VFV30_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4847110.1"
                     /translation="MDRKLGYWALLALVIGNMVGSGIYVLPAQLAPLGWNQFVGWGIT
                     IVGALALAQVFSALGARLPLAGGPYAYASAAFGPLAGFTTAWAYWVMSWVGNAAVAVA
                     VVSALALIFPELGSTQGLPAVLAVACVWVVTLVNIRGVSLAGRLQEVTVVLKLLPLVG
                     LIGIAVWLWLTGAERAPDPQVPFTADNIALAAGLTFWGFLGLEAATVPADKVENPARN
                     IPRATMIGVALTGLIYIGISGAFALFMPIDEAAASPAPIASFLGRYLGGDVAIVVALF
                     AAISAFGTLNGWVLVQAEMPWAMAKGGVFPQWFAVEGKHGTPVRSHLVSSSLLSVITL
                     LNYQKGMTDLFGFIASVSLAAGLVAYLMAALAALRLIRDQLLTAFAALVAVAFFCWAE
                     YGLGQEAMLYAGLLILGGLPVYWQVRSAKSSG"
     gene            complement(2241..>3159)
                     /locus_tag="VFV30_03120"
     CDS             complement(2241..>3159)
                     /locus_tag="VFV30_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4847111.1"
                     /translation="VNGKLGAWGHSVGGMGAITQAMARACEDAGVEISLEAPVAKVLV
                     DGGKVKGVQLESGEEIAASIVASNAGPALLYRQLVDESDLDADFKRRIKGYKTCSGTF
                     RMNVALSELPDFSVLPGKEQAEHHTAGIIIAPGMDYMDQAFIDAQQHGWSKKPIVEIK
                     IPSTVDDSLAPPGCHVASLFCQQFDPTVDWDANREKVADLIIDTVTDHAPNFKKSVIA
                     TQIHSPWDLERKFGLIGGDIMHGTMGIDQLWAARPVLGHGDYRGPIKGLYMCGAGTHP
                     GGGVTGAPGHNAAQAILADRPFIRKILRS"
BASE COUNT          430 a         1083 c         1065 g          581 t
ORIGIN      
        1 gaccagggtc gatccggcgc gcggcatccc cagcacaaag atcggatcgg gcgcgtcgca
       61 gccctgccct tctcgggccg cgaagtatgc gggcgtcgcc acttcgatca gccggtcgac
      121 cagccgctcg ttctcgtccg cttcgtaatc gagatcggtc ttcctcagcg cgttggcggc
      181 ggcgtagtgg gcaaaggcgg catcggtctc gccccggtcc tccagcgcct tgcccagcgc
      241 gaaatcgagg tggaagcggt cgtccttggc aacatcgccg ctggccagcg cggcctccat
      301 cgcggcgata tccgcgtcat cgaaccggac cgtcttgagg ttggccaggc tccaccaggc
      361 ttcgcccagg ctgggcatca gttcaatcgc cttgcggtag gccgcgatcc cttcgtcgag
      421 ccggccgacg gtcttgagca tatggccaaa gctcatccac acgcgcggct gctgcggggc
      481 cttttccagc accttctcgt acagcgggat cgcctcttcg aactcgccga tccggcccag
      541 ggccgcggcc tgaaggttgg cgtggccggg gttctccggc tcttcggcaa tcacccggtt
      601 gagctcggca atcgcatcgg ccggccggtt ctggcggtaa agcaccagcg ccaggttggc
      661 ccgcgcggcg gtgaagccgg gcgacagttc cagcgcgcgg cgcagcagcg tctccgaatc
      721 cttgtagcgc ccgatccgcc ccgccacttc ggccagcatc cggatcgcgg cgacttcgaa
      781 ggggcgctcc ttcagatggg tgcgcagcag gaattcggcc ttggggatgt cgttcgcgtt
      841 catcgccacc gccgcctcga tcaggcgcgg gtggtactgg ccgcggcgcg aaagccagct
      901 ctggttggta agggttgcca tgggccgatc aagaaacatg gtgacgcgcc gcgatcaagc
      961 gataggttgc gcggggaagg ggataaagcc gatggatcgc aagcttggct actgggcgct
     1021 gctcgccctg gtgatcggca acatggtggg ttcggggatc tatgtcctgc ccgcacagct
     1081 ggcgccactg ggctggaacc agttcgtcgg ctgggggatc accatcgtcg gagcccttgc
     1141 gctcgcccag gtcttttccg cgctgggcgc ccggctgccg ctggccggcg ggccctatgc
     1201 ctatgccagc gccgccttcg ggccgctggc cggcttcacc acggcctggg cctattgggt
     1261 gatgagctgg gtcggcaacg ccgccgtggc cgtggccgtg gtcagcgccc tggcgctgat
     1321 ctttccggag ctgggcagca cgcagggcct gcccgcggtg ctggccgtgg cctgcgtctg
     1381 ggtggtgacc ctggtcaaca tccgcggcgt ctcgctggcc gggcgcctgc aggaagtgac
     1441 cgtggtgctg aagctgctgc cgctggtcgg cctgattggc atcgcggtgt ggctgtggct
     1501 gaccggggcg gagcgcgcgc ccgatccgca ggtgcccttc acggcggaca acatcgcgct
     1561 ggccgccggc ctcaccttct ggggcttcct ggggctggaa gcggccacgg ttccggccga
     1621 caaggtggag aacccggccc gcaacattcc ccgcgcgacg atgatcggcg tggcgctgac
     1681 cggcctgatc tacatcggca tttcgggcgc ctttgccctg ttcatgccga ttgacgaggc
     1741 cgccgcctcc cctgccccga tcgccagctt cctgggccgc tacctgggcg gcgatgtcgc
     1801 catcgtggtg gcgctgttcg ccgcgatcag cgctttcggt acgctcaacg gctgggtctt
     1861 ggtccaggcc gaaatgccct gggccatggc caagggcggc gtctttccgc aatggttcgc
     1921 ggtggagggc aagcacggca ccccggtccg ctcgcacctc gtctccagca gcctgctctc
     1981 ggtcatcacc ctgctcaact accagaaggg catgaccgac ctgttcggct tcatcgcctc
     2041 ggtcagcctg gccgccggtc tggtcgccta cctaatggcc gcgctggccg cgctgcgcct
     2101 gatccgcgac cagctgctga ccgcctttgc cgcgctggtc gcggtggcgt tcttctgctg
     2161 ggccgaatac ggcctggggc aagaagcgat gctctatgcc ggcctgctga ttctgggcgg
     2221 cctgccggtc tactggcagg tcaggagcgc aaaatcttcc ggatgaacgg ccggtcggcc
     2281 aggatcgcct gggccgcgtt gtggcccggc gcgccggtca cgccgccgcc cggatgggtt
     2341 cccgccccgc acatgtagag gcccttgatg ggcccgcggt aatcgccatg gcccagcacc
     2401 gggcgcgccg cccacagctg gtcgatcccc atcgtgccgt gcatgatgtc cccgccgatc
     2461 aggccgaact tgcgctccag atcccacggc gagtggatct gcgtggcgat gaccgacttc
     2521 ttgaagttgg gcgcgtgatc ggtaaccgtg tcgatgatca ggtcggccac cttttcgcgg
     2581 ttggcatccc agtccacggt cgggtcaaac tgctggcaga acagcgacgc aacgtggcag
     2641 cccggagggg ccaggctgtc gtccactgtc gaggggatct tgatctcgac gatcggcttc
     2701 ttcgaccagc cgtgctgctg ggcgtcgatg aaggcctggt ccatatagtc catgcccggc
     2761 gcgatgatga tcccggcggt gtggtgctcg gcctgctcct tgcccggcag cacgctgaaa
     2821 tcgggcagct cggacagcgc gacgttcatc cggaaggtgc cggaacaggt cttgtagccc
     2881 ttgatccgcc gcttgaaatc ggcgtcgagg tcgctctcgt cgaccagctg gcggtagagc
     2941 agcgccggcc cggcgttgga ggcgacgatg ctggcggcga tttcctcgcc gctttccagc
     3001 tgcacgccct tcaccttccc gccatcgacc agcaccttgg cgaccggcgc ctccaggctg
     3061 atctcgaccc cggcatcctc gcaggcccgg gccatggcct gggtgatcgc gcccatcccg
     3121 ccgaccgaat ggccccaggc gcccagcttg ccgttcact
//
LOCUS       DASUDV010000144         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_932300,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000144 DASUDV010000000
VERSION     DASUDV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932300"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..512
                     /locus_tag="VFV30_03125"
     CDS             54..512
                     /locus_tag="VFV30_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847112.1"
                     /translation="MTDLSLRAATPADLAALLRLHLEEMHRWSPPESVHAMPIDRLRA
                     ADVTFFTAWHGDQLAACGAVKHLADDHGELKSMRADPAYRGKGAGKAILLHLLGEARR
                     RGYARVSLETGRPDEFLPARRLYESHGFAECPPFASYTEDPFSICMTRAL"
     gene            509..1030
                     /locus_tag="VFV30_03130"
     CDS             509..1030
                     /locus_tag="VFV30_03130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847113.1"
                     /translation="MTDLSLRAATPADLESLSELARDAFIAKFGHLYSQANLDLFLAE
                     ALSEAAFAADLADPDRAIQLAFRGDALVGFAKVAFKCGFPDHARGSRTMELKQLYTGA
                     QATGGGIGSALMDWAMASLTARGADEVQLSVYAENFGAHRFYERYGFAKVADITFRVG
                     EHIDPEFLFARMT"
     gene            complement(1027..1797)
                     /locus_tag="VFV30_03135"
     CDS             complement(1027..1797)
                     /locus_tag="VFV30_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4847114.1"
                     /translation="MIRRVATAIAALFLSQAALAEPIPATIEFRGTISASAPDTLIVN
                     GQPWTGPMPDYPYKAGDQISVSFVATPGSAVQSADGLYRYTIYGASQSGSISGSNYAR
                     AGTIDVSGPIAGGDFVSGMTLVYDAVADSYTIELPQDRWIMSSFDGPGYLYDPANNSL
                     SSTSTTRQPGGFCGDGGAGCFAISGNLSGGAVDRAPVWGTDGSLRGFFSMLFSGDWFV
                     NGSRVGGATQVPEPGQLALMMAALVPLVLRRRRKLATG"
     gene            1925..>2101
                     /locus_tag="VFV30_03140"
     CDS             1925..>2101
                     /locus_tag="VFV30_03140"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX4847115.1"
                     /translation="MSSIRPWRDIARRECRQIMVGKVPVGGNAPITVQTMTNTLTSDA
                     RATIDQIRRCEEAGA"
BASE COUNT          389 a          694 c          648 g          370 t
ORIGIN      
        1 ccgttcgcta gctacaccga agaccccttt tcgatttgca tgacgagagc cctttgaccg
       61 accttagcct gcgtgccgca acccctgccg atcttgccgc gctgctgcgc ctgcatctcg
      121 aagaaatgca ccgctggtcg ccgccggaaa gcgtccatgc catgccgatt gaccggttgc
      181 gcgcggccga tgtcaccttc ttcaccgcct ggcacggcga tcagctggcg gcctgcgggg
      241 cggtcaagca ccttgccgac gatcacggcg agctcaaatc gatgcgcgcc gatccggcct
      301 atcgcggcaa gggggcgggc aaggcgatcc tgctgcacct gctgggagaa gcgcggcgtc
      361 gcggttatgc ccgggtcagc ctggagaccg gtcggccgga cgaattcctg cccgcccggc
      421 ggctttatga aagccacggc tttgccgagt gcccgccgtt cgctagctac accgaagacc
      481 ccttttcgat ttgcatgacg agagcccttt gaccgacctg agcctgcgcg ccgcgacccc
      541 tgccgatctt gaatccctgt ccgaactggc gcgcgatgcc ttcatcgcca agttcgggca
      601 tctctacagc caggcgaacc ttgacctgtt cctggctgag gcgctcagcg aagcggcctt
      661 tgccgccgat ctggccgatc cggatcgcgc gatccagctg gcgttccggg gtgacgcgct
      721 ggttggcttt gccaaggtgg ccttcaagtg cggttttccc gatcatgccc ggggcagcag
      781 gaccatggag ctcaagcagc tttataccgg ggcgcaggcc accggcgggg ggattggctc
      841 ggccttgatg gactgggcga tggccagtct gaccgcccgc ggtgccgatg aagtccagct
      901 gtcggtctat gccgaaaact tcggcgcaca ccggttctac gaacgctatg gctttgccaa
      961 ggtggccgac atcaccttcc gggtcggcga acacatcgat cccgaattcc tcttcgcacg
     1021 gatgacctag cctgtcgcca acttgcgccg acgacgcagc accagcggga caagcgccgc
     1081 catcatcagc gcaagctggc cgggctcggg cacctgggtt gctccgccaa ccctgctgcc
     1141 gttcacgaac cagtcgcccg aaaacagcat gctgaagaac ccgcgcagcg aaccgtcagt
     1201 gccccagacc ggggcgcgat ctaccgcccc gccgctaagg ttgccggaaa tggcgaagca
     1261 acccgccccg ccatcgccac aaaagccgcc gggctgccgg gtggttgaag tggaagacag
     1321 gctgttattg gccgggtcat agagatagcc cggtccatcg aacgacgaca tgatccagcg
     1381 atcctgcggc agttcgatcg tatagctgtc ggccacagcg tcataaacca gcgtcatccc
     1441 gctaacgaag tcgccacccg caatcggacc gctcacatcg attgttcccg cgcgggcata
     1501 gttgctgccc gaaatgctgc cgctctggga cgcgccatag atggtatagc ggtagagacc
     1561 atcggccgac tgcaccgccg aacctggcgt ggcgacgaac gagaccgaga tctgatcgcc
     1621 ggccttgtag ggataatcgg gcatggggcc tgtccagggc tggccattga cgatcagcgt
     1681 atcgggcgcc gaggccgata tcgttcccct gaattcgatc gtggcgggaa tcggctcggc
     1741 cagggcggcc tgcgacagga ataaagcggc gattgcggtt gcgacccgac gaatcatgat
     1801 aacctccctg aatttcagag tcttatgagt gtgcggcaat ggctggcagg ccaatcgcgc
     1861 aatccgcgcc gtttcattgt ggcacgcaat tgccgtcgca cgcttggccg cctatatcgc
     1921 gcccatgtca tcgatcagac cctggcgcga tatcgcgcgg cgcgaatgca gacagatcat
     1981 ggtgggcaag gtccccgtgg gcggcaacgc gccgatcacg gtccagacca tgaccaacac
     2041 gctgaccagc gatgccaggg cgacgatcga ccagatccgc cgctgcgagg aagccggcgc
     2101 c
//
LOCUS       DASUDV010000145         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1263465,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000145 DASUDV010000000
VERSION     DASUDV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263465"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VFV30_03145"
     CDS             <1..120
                     /locus_tag="VFV30_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX4847116.1"
                     /translation="YTPRYLWWNFVSSSQERIRAATRDWQQGRFPPVPGDPDT"
     gene            211..1032
                     /locus_tag="VFV30_03150"
     CDS             211..1032
                     /locus_tag="VFV30_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847117.1"
                     /translation="MTTSTKAVWITGASSGIGAALARAYARRGCRLILSGRNEAALAE
                     VAAQCGVDHLILPFEVSDFAAGLAAADKAWEWSGGIDVLVNNAGISQRSLAIDTDFAV
                     YQKMIAIDLLAPINLTQALIKRMAARKTGQFIMISSLAGKIGSPLRSAYCAAKHGLVG
                     YADSIRLELNQHGISVIVATPGSIRTDVSRNAITSDGSTRGVSDEAIDNGVEPDDFAE
                     QMLAAIDAGEREIVVAKHPMEVMIGEARRTPEALFDKMIDSFNAGYAAKMGVSEQ"
     gene            1035..1937
                     /locus_tag="VFV30_03155"
     CDS             1035..1937
                     /locus_tag="VFV30_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847118.1"
                     /translation="MEQTRVRLASGIELDVVDCGPRDAPALIFLHGFPESHRTWRHQI
                     AHLSDRYRCIAPDQRGYRGSSKPQEVEAYTPDKLIGDVFQLADALGIDRFTVLGHDWG
                     GAIAWGVALTGQMNGRVTRAVIANAPHPVGFSRLVWLNRHQRESSQYMRTFRDTANDG
                     LVREHGLGGMLLKAFAGRENTEDKMEPAERAQLLLDWENREAAFGMLNWYRASPLYVP
                     AMDEPYEIPADRQPPPIPPLAIPTLVIWAMDDFALPPENLDGLDAMVPAMTLVKVPGC
                     GHFVPWEAPQAVNAALDEFLGRTG"
     gene            1921..>2165
                     /locus_tag="VFV30_03160"
     CDS             1921..>2165
                     /locus_tag="VFV30_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX4847119.1"
                     /translation="MAEPAKPARPAGGIGAAVAAAERRARRSLLRGHGGGHAPVSNLE
                     LFFDLVFVFAITQLSHFLGHHLGWLGAFEALMLFLAL"
BASE COUNT          378 a          699 c          706 g          382 t
ORIGIN      
        1 tatacgccgc gctatctgtg gtggaatttc gtgtcctcat cgcaggaacg gattcgcgcg
       61 gccacgcgcg attggcagca gggccgcttc ccgcccgtgc ctggcgatcc ggacacctga
      121 tcgcgccaat cggcaatcga gggttgcgca ccgggcgcgg gcttgcttcg ctgctcggca
      181 gaaccgaatc gagtgacagg gagcgctgga atgaccactt cgaccaaagc agtctggata
      241 accggcgcat catccgggat cggcgcagcc ctggcccggg cctatgccag gcgcggatgc
      301 cgactgatcc tttcgggccg gaacgaggca gctctggccg aggttgccgc gcaatgcggc
      361 gtcgatcacc tgatcctgcc attcgaggtg tcggactttg ccgcagggct cgccgccgcc
      421 gacaaggcct gggaatggag cggtgggatc gacgttctgg tcaacaatgc cgggatcagc
      481 cagcgcagcc tggcgattga taccgatttt gccgtgtacc agaagatgat cgcgatcgat
      541 ctgctcgccc cgatcaacct gacccaggcg ctgatcaagc ggatggcggc gcgcaagacc
      601 gggcagttca tcatgatttc gagccttgcc ggcaagatcg gctcgccgct gcgctcggcc
      661 tactgcgcgg ccaagcacgg cctggttggt tatgcggatt cgatcaggct cgaattgaac
      721 cagcacggca tttcggtgat cgttgccacc ccgggatcga tccgcaccga tgtctcgcgc
      781 aatgccatca cctccgatgg gtcgacccgc ggagtgagcg atgaagcgat cgacaacggc
      841 gtcgaaccgg atgatttcgc cgaacagatg ctggccgcaa tcgatgccgg ggaacgcgag
      901 atcgtggtgg ccaagcaccc gatggaggta atgatcggcg aggcgcgccg cacgcccgag
      961 gcgctgttcg acaagatgat cgatagtttc aacgccggct atgccgcgaa gatgggcgtg
     1021 agcgaacagt gagcatggaa cagaccaggg tccgcctggc cagcgggatc gaactcgatg
     1081 tggtcgactg cggcccgcgc gacgcacccg cgctgatctt cctgcatggc tttcccgaaa
     1141 gccaccgcac ctggcgccac cagatcgcgc atctttccga tcgctatcgc tgcattgcgc
     1201 ccgatcagcg cggctatcgc ggctcgtcca agccgcaaga ggtcgaagcc tacacccccg
     1261 acaagctgat cggcgacgtg ttccagctgg ccgatgccct ggggatcgat cgcttcacgg
     1321 ttctcggcca cgattggggc ggcgcgattg cctggggcgt ggccttgacg ggacagatga
     1381 atggccgggt aacccgcgcg gtgatcgcca atgcgccgca tccggtgggc ttctcgcggc
     1441 tggtctggct caaccgccat caacgcgaat cgagccagta catgcggacc ttccgcgata
     1501 cggcaaacga tgggctggtg cgcgaacacg gcctgggcgg aatgctgctg aaggcctttg
     1561 ccggccggga gaataccgaa gacaagatgg aaccggccga gcgggcgcaa ctgctgctag
     1621 attgggaaaa ccgcgaagcc gcctttggca tgctcaactg gtaccgggcc agcccgctct
     1681 acgtgccggc catggacgag ccctacgaga tacccgccga tcgccagccc ccgccgatcc
     1741 cgccgcttgc cattcccacc ctggtgatct gggcgatgga cgatttcgcg ctgccgcccg
     1801 aaaatctcga tgggctggac gcgatggtgc cggccatgac cctggtcaag gtgcccggtt
     1861 gcggccattt cgtgccctgg gaagcgccgc aggcggtcaa tgccgcgctg gacgaattcc
     1921 ttggccgaac cggctaagcc cgcccgcccg gcaggcggaa ttggcgctgc ggttgcagcc
     1981 gcggaacgcc gtgcccgccg ctcgctgctg cgcggccacg gcggcgggca tgccccggtc
     2041 agcaatctcg aactgttctt cgatctggtc ttcgtcttcg cgatcaccca gctgtcgcac
     2101 tttctgggcc accacctcgg ttggctgggc gccttcgaag cgctgatgct gtttctggcg
     2161 ctgtg
//
LOCUS       DASUDV010000146         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2036946,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000146 DASUDV010000000
VERSION     DASUDV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036946"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFV30_03165"
     CDS             complement(<1..546)
                     /locus_tag="VFV30_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4847120.1"
                     /translation="MPSLPPFDSNAAIPFHAAGTRLPVGLAGELRIWGRSALLALAML
                     VLVPLHYLFRVIKYGSPFPMYFLRWAAWVIGARITVHGVPLKRDVFFMPNHASWFDIP
                     VLGGLTGSAFVSRAEIAEMWMLGWMARLNRTIFVKREARMNIAEQINDLREAVADTWS
                     VVIFPEGTVTDGHSLLPFKTSM"
     gene            complement(561..1322)
                     /locus_tag="VFV30_03170"
     CDS             complement(561..1322)
                     /locus_tag="VFV30_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4847121.1"
                     /translation="MPIAIAPPRTMSVMGRIRIVLRLVAMLAWLIAAIPAYYLFAAFT
                     RHNPVPRAFLSGINRIAGVRLIVHGEKVHRGAFLLANHVSWIDIPALSATTGTAFVGH
                     DGLASMPLLKWLCKMNDTVFVARHDRTSVARQIEDVRTALSDTGALAVFPEGTTSDGT
                     GLLQFKSSLLSALEPLPAGIAIQPVLLDYGPEAAEIAWVGEEHGLHNFLRILARRRPV
                     VVTVRFLPVLAGDQLANRKTIAQAAREAILNRLAG"
     gene            complement(1322..1744)
                     /locus_tag="VFV30_03175"
     CDS             complement(1322..1744)
                     /locus_tag="VFV30_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX4847122.1"
                     /translation="MHQQIDIEALCAERGLRITEQRRVIAKVLSESTDHPDVEKLHER
                     AVAVDPRISIATVYRTVRLFEEAGILDRHDFGDGRARYEAAPEAHHDHLIDVETGKVI
                     EFVDPELEALQRQIAERLGYRLVDHRMELYGVRIGREG"
     gene            complement(1828..>2021)
                     /locus_tag="VFV30_03180"
     CDS             complement(1828..>2021)
                     /locus_tag="VFV30_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX4847123.1"
                     /translation="LKRHLMTHYNMTPEQYRARWNLPADYPMVAPDYAEKRRELAKKI
                     GLGRKPGQKRGRKPKAAKA"
BASE COUNT          364 a          632 c          686 g          339 t
ORIGIN      
        1 catcgaagtc ttgaacggca gcagcgaatg gccatcggtg accgtgcctt cggggaagat
       61 caccaccgac caggtatcgg ccacggcttc gcgcagatcg ttgatctgtt cggcgatgtt
      121 catccgcgcc tcgcgcttga caaagatcgt gcggttgagc cgcgccatcc agcccagcat
      181 ccacatctcg gcgatttcgg cgcgcgagac gaaggccgaa ccggtaagcc cgcccagcac
      241 cgggatatcg aaccacgagg cgtggttggg catgaagaat acgtcgcgct tcaacggaac
      301 gccatgaacc gtgatccgcg cgccgattac ccaggccgcc cagcgcagga agtacatcgg
      361 gaagggcgag ccgtatttga tcacccggaa caggtagtgc agcggcacca agaccagcat
      421 ggccagcgcc agcagcgccg accggcccca gatcctgagt tcaccggcga ggcccaccgg
      481 caggcgcgtg ccggcggcgt ggaacgggat cgccgcgttc gaatcgaaag ggggcaggct
      541 gggcatgatg ggtctttcga tcagccggcc agccggttca ggatggcctc gcgcgcggcc
      601 tgggcgatgg tcttgcggtt ggcaagctga tcgccggcga gcaccggcag gaaccgcacc
      661 gtcaccacca ccgggcggcg ccgggccagg atgcgcagga agttgtgcag gccgtgctct
      721 tcgccaaccc aggcgatctc ggcggcctct ggcccatagt cgaggagcac cggctggatc
      781 gcgattcctg cgggcagcgg ctcgagcgcg gagagcagcg agctcttgaa ctgcaacagg
      841 ccggtgccat cgctggtcgt gccttcggga aacaccgcca gcgccccggt atcggacagc
      901 gcggtgcgca catcctcgat ctggcgggcg acactggtgc gatcgtgccg cgcgacgaag
      961 accgtgtcgt tcatcttgca cagccatttg agcagcggca tcgatgccag tccgtcgtgt
     1021 ccgacaaagg cggtgccggt ggtggccgaa agcgcgggga tatcgatcca gctgacatgg
     1081 ttggcgagca ggaaggcgcc gcgatgtacc ttttcgccgt ggacgatcag ccgtaccccg
     1141 gcgatgcggt tgattcccga aaggaaggcg cgcggtaccg ggttgtggcg ggtgaaggca
     1201 gcgaaaaggt agtaggccgg gatcgcggcg atcagccagg ccaacatcgc cacgagccgc
     1261 agcacaatcc ggatccgccc catgacggac atggtccggg gcggggcgat ggcgatcggc
     1321 atcagccttc ccggccgatc ctgacgccgt aaagttccat gcggtgatca accaggcgat
     1381 agcccagccg ttcggcgatc tggcgctgca gcgcctcaag ctcggggtcg acgaattcga
     1441 tcaccttgcc ggtttcgaca tcgatcagat ggtcgtggtg ggcctcgggc gcggcttcat
     1501 agcgggcgcg gccatcgccg aaatcgtggc gatcgaggat cccggcctct tcgaacagcc
     1561 gcacggtgcg atagaccgtg gcgatcgaaa tgcgcggatc gacggcgacc gcccgctcgt
     1621 gcagcttttc cacatccgga tggtcagtgc tttcggacag caccttggcg atcacgcggc
     1681 gctgctccgt gatgcggagg ccgcgctcgg cgcaaagggc ttcgatgtcg atctgctggt
     1741 gcactggcaa tccatgaatg aaaagtcccg ccggatatga acccggcggg acgattcttc
     1801 aaccgcgcgt ggcggactta tccggcctca ggccttggct gccttgggct tgcgaccacg
     1861 cttctggccg ggcttgcggc ccaggccgat cttcttggcc agttcgcggc gcttttcggc
     1921 atagtccggg gcgaccatcg gatagtcggc gggcaggttc cagcgcgcac gatactgttc
     1981 cggggtcatg ttgtaatgcg tcatcaggtg acgcttcagc a
//
LOCUS       DASUDV010000147         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_667171,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000147 DASUDV010000000
VERSION     DASUDV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667171"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1063
                     /gene="ilvC"
                     /locus_tag="VFV30_03185"
     CDS             44..1063
                     /gene="ilvC"
                     /locus_tag="VFV30_03185"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX4847124.1"
                     /translation="MKVYYDADCDLNLVTGKKIAVLGYGSQGHAHAQNLRDSGVKDVA
                     IALRPGSASAKKAEAAGFKVLANADAAKWADIVMILAPDEHQAAIWENDLKGNMRPGS
                     ALAFAHGLNIHFGLIEAPADIDVIMIAPKGPGHTVRGEYLKGGGVPCLIAVHQDATGN
                     AHDVALAYASGVGGGRSGIIETNFKEECETDLFGEQAVLCGGITHLIQAGFETLVEAG
                     YAPEMAYFECLHETKLIVDLLYEGGIANMRYSISNTAEYGDITTGPRIITADTKAEMK
                     RVLADIQSGRFVKNFVLDNRAGQPELKAARKAAEAHPIEQTGSRLRAMMPWIGKNKLV
                     DQEKN"
     gene            1134..1784
                     /locus_tag="VFV30_03190"
     CDS             1134..1784
                     /locus_tag="VFV30_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX4847125.1"
                     /translation="MPHLRLKTVPLAVIGAALVAVAALPAQTGKLSGAADPARVAAGT
                     YTADPAHTLIGWRVSHMGFNDYFGLFGSITGSLTIDPARPEAASVTITVPVRKVTTAD
                     RILTSRLLQPRISGKPDYFGPEPKDATFVSTRVVPGADGKTATIEGNLTLNGATRPVS
                     IAATFAGAGKNPYTGKATVGFHGLTKIKRSDFGLTADMTLVGDEVELTITAAFEKR"
     gene            complement(1781..2905)
                     /locus_tag="VFV30_03195"
     CDS             complement(1781..2905)
                     /locus_tag="VFV30_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX4847126.1"
                     /translation="MTQIRPFTLAVPQSDLEDLHRRLDQTRWPEKEAVDDWSQGTPLA
                     ALQDLVAYWRKDYDWRRCEARLNALGQFKTEIDGLDIHFLHIRSPHAGALPMVMTHGW
                     PGSVIEFMGVIEALTNPEDPAQAFDLVLPSLPGFGFSGKPTVTGWGVEKIGRAWGELM
                     TRLGYSHWVAQGGDWGSAVTTAIGVQKLPGCAGIHLNMPIGRPEPEDLANPTPAELKA
                     LGALKHYQDWDSGYSTQQRTRPQTVGYGLVDSPVGLAGWIFEKMWAWTDNAGSPFDAL
                     SRDQILDNIMLYWLPATGASSARLYWESFGSFAPQAIELPVAVSAFPREILPTPRKWA
                     ERNYRELVHWGDMDKGGHFAAWEQPAAFVSELRTAFGRMR"
     gene            2991..>3198
                     /locus_tag="VFV30_03200"
     CDS             2991..>3198
                     /locus_tag="VFV30_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4847127.1"
                     /translation="MVQPQLAGIKVVDLTTVVFGPYRTQILADLGAEVIKVETPGHGD
                     AFRWSAQAAATPGMAPGFMAVNRGK"
BASE COUNT          583 a         1106 c         1025 g          484 t
ORIGIN      
        1 gccccgccag acggggccac actgcaagag aacgggaact accatgaagg tttactacga
       61 cgccgattgc gacctgaacc tggtcacggg caagaagatc gccgtcctcg gctatggcag
      121 ccagggccat gcccacgcgc agaacctgcg cgacagcggg gtcaaggacg tcgccatcgc
      181 gctgcgcccc ggttcggcca gcgccaagaa ggccgaagcc gccggcttca aggtccttgc
      241 caatgccgac gccgccaagt gggccgacat cgtgatgatc ctggcccccg acgaacacca
      301 ggccgcgatc tgggagaacg acctcaaggg caacatgcgc ccgggttcgg ccctggcctt
      361 cgcccacggc ctcaacatcc atttcggtct gatcgaagcc cccgccgaca tcgacgtgat
      421 catgatcgcg ccgaagggcc ccggccacac cgtgcgcggc gaatacctga agggcggcgg
      481 cgtgccgtgc ctgatcgcgg tccaccagga cgccaccggc aacgcccatg acgtcgccct
      541 ggcctatgcc agcggcgttg gcggcggccg cagcggcatc atcgaaacca atttcaagga
      601 agagtgcgaa accgacctgt tcggcgagca ggccgtgctg tgcggcggga tcacccacct
      661 gatccaggcc gggttcgaaa ccctggtcga agccggttac gcccccgaaa tggcctattt
      721 cgagtgcctc cacgaaacca agctgatcgt cgacctgctc tatgaaggcg gcatcgccaa
      781 catgcgctat tcaatcagca acaccgccga atacggcgac atcaccaccg gcccgcggat
      841 catcaccgcc gataccaagg ccgagatgaa gcgcgttctg gccgacatcc agtcgggccg
      901 gttcgtcaag aacttcgtgc tcgacaaccg cgccggccag ccggagctga aggccgcccg
      961 caaggccgcc gaagcccacc cgatcgaaca gaccggcagc cgcctgcgcg cgatgatgcc
     1021 gtggatcggc aagaacaagc tggtcgatca ggaaaagaac tgatccttcg ttcagcttga
     1081 ccacaggttg ggcaaggcac acaccggggc tccttttcgc aggagccccg accgtgccgc
     1141 accttcgcct caaaaccgtc ccgcttgccg tcatcggcgc cgcgctggtc gcagtcgccg
     1201 cgcttcccgc gcagaccggc aagctgtcgg gcgccgcgga tccggcccgt gttgccgccg
     1261 gcacctatac cgccgatccc gcccacaccc tgatcggctg gcgcgtcagc cacatgggct
     1321 tcaatgacta tttcgggctg ttcggatcga tcaccggcag cctgacgatc gaccccgccc
     1381 ggcccgaagc ggccagcgtg acgatcaccg tcccggtccg caaggtcacc accgccgacc
     1441 ggatcctgac ctcgcgcctg ctccagccgc ggatcagcgg caagcccgac tatttcgggc
     1501 ctgagcccaa ggacgcgacc ttcgtttcca cccgcgtggt ccccggcgcc gatggcaaga
     1561 ccgcgacgat cgagggcaat ctgacgctga acggcgcgac ccgcccggtc tccatcgccg
     1621 cgacgttcgc cggggccggc aagaacccct acaccggcaa ggccacggtc ggctttcacg
     1681 ggctgacgaa gatcaagcgc tccgactttg gcctgacggc cgacatgacg ctggtcggcg
     1741 acgaggtaga gctgaccatc accgcggcgt tcgaaaagcg ctaacgcatc ctgccgaagg
     1801 cggtgcgcag ttcggagacg aatgcggccg gctgttccca ggcggcaaag tggccgccct
     1861 tgtccatatc gccccagtgc accagctcgc ggtaattgcg ctcggcccat ttgcgcgggg
     1921 tcggcaggat ttcccgggga aaggcgctga ccgcaaccgg cagctcgatc gcctgcggag
     1981 cgaagctgcc gaagctttcc caatagagcc gggccgaaga ggccccggtg gccggcagcc
     2041 agtagagcat gatgttgtcg aggatctggt cccggctgag cgcgtcgaac ggggatccgg
     2101 cgttgtcggt ccaggcccac atcttctcga aaatccagcc ggccagcccg accgggctgt
     2161 cgaccagacc gtaaccgacc gtctgcggcc gggtgcgctg ctgggtggag tagcccgaat
     2221 cccagtcctg gtaatgcttg agcgcgccca gcgccttgag ctcggccggg gtgggattgg
     2281 ccagatcctc aggctccggc cgcccgatcg gcatgttgag gtggatcccc gcgcagcccg
     2341 gcagcttctg cacgccgatc gccgtggtca ccgcagagcc ccagtcgccg ccttgagcca
     2401 cccagtggct gtagcccaac cgggtcatca gttcgcccca ggcgcggccg atcttttcga
     2461 cgccccagcc ggttaccgtg ggcttgccgg aaaagccgaa gcccgggagc gagggcagca
     2521 ccaggtcgaa ggcctgggcg ggatcctcgg gattggtcag cgcctcgatc acgcccatga
     2581 attcgatcac agatccgggc cagccgtggg tcatcaccat cggcagggcc ccggcgtggg
     2641 gcgagcggat gtggaggaag tggatgtcga gcccgtcgat ctcggtcttg aactggccca
     2701 gcgcgttgag ccgcgcctcg caccgccgcc agtcatagtc cttgcgccaa taggcgacca
     2761 ggtcctgcag ggcggcgagc ggcgtccctt gcgaccaatc gtccaccgcc tccttctctg
     2821 gccagcgggt ctggtcaagc cggcgatgga gatcctcaag atcggactgc gggacggcga
     2881 gcgtgaaggg gcgaatctgg gtcatgccgg caggctaggc caagttcggc gttgtgttaa
     2941 ccgttcgatt aaagataatc ggggccgcgt ataacgggag agcatccgac atggtccagc
     3001 cgcagctagc cgggatcaag gtggtggacc tgaccaccgt ggtctttggc ccctatcgca
     3061 cccagatcct ggccgatctg ggggcggagg tgatcaaggt cgaaaccccg gggcacggcg
     3121 atgccttccg ctggtcggcc caggcggccg ccacgcccgg catggcgccg gggttcatgg
     3181 cggtgaaccg cggcaaac
//
LOCUS       DASUDV010000148         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2191838,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000148 DASUDV010000000
VERSION     DASUDV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191838"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..824)
                     /locus_tag="VFV30_03205"
     CDS             complement(15..824)
                     /locus_tag="VFV30_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX4847128.1"
                     /translation="MPGTPTSRSPLKLALFATASAAMLVTAGCAGRGNQAKDTAFVAR
                     DVDTLYMAAKDRLDRGDTKLAAALFDEVERQHPYSPWARRAQLMSAFSYYVARDYTKA
                     IQSAQRFLSIHPGNRDAPYAYYLIGLSYYEQISDVSRDQKITRLALDSMNEVVRRYPN
                     TRYASDARLKIDLINDHLAGKEMEIGRFYQRSGRWLAANLRFRNVIEGYQSTSHTPEA
                     LYRMVESNLAIGVPAEAQKAAAVLGANYPGSEWYKKAFDLMQKYAPNTAAI"
     gene            complement(901..2853)
                     /locus_tag="VFV30_03210"
     CDS             complement(901..2853)
                     /locus_tag="VFV30_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HEX4847129.1"
                     /translation="MPHQAPAHPSLPQNDTPAQQAARAAQLVAAQGVYRWTTAVPSLP
                     GAPLLDGQPPANEQPTLTWLILLADELLAIVKNHLAVKKLWAEQALLPAAPAAIGAEI
                     AALAAIELAIDGIRSKIAHNTSGGTIWSRIAEDIELALGAAGAVALDAALRDQLAHLF
                     EVVSLSAAEHAALGSRTPRDLERYRELFQTLTLPGIGWTFQDDAEFARLRVAGPNAAL
                     IKRIAALPENFALTAERYAAVVAGDDLNTALAAGRIYLCDYAALSKLVPGSYQGQAKY
                     AYRPLALFAVPPGGASLVPVAIQCGQDGSVDPVFTPSVRPDEQWGWDIAKFIVQVADG
                     NYHELIAHLAHTHLVIEALALATRRHLAEVHPLWALLVPHYEGTLFINNAAATSLITP
                     GGPIDHIFAGTIASSQAQAVAARLEFDFTRRMLPHDLKERGVDDAGALPDYPYRDDAL
                     LVWQAIREWVLGYVGLYYASDADVAGDPELAAWCAELQGAGQLAGFPTITTAAALVDA
                     CTMILFTCSAQHAAVNFPQKDVMEFAPAVTGAGWADAPLERQGHDRAEWLGYMPPLAP
                     ALQQLETLYLLGSLHYRPLGTYQSRNFPYPAWFQDLQVTGPEGPLARFQASLQGVEQR
                     IAARNAARRVAYPYLLPSRIPTSTNI"
BASE COUNT          525 a         1019 c         1056 g          528 t
ORIGIN      
        1 cggctagacc cggcttagat cgcggccgtg ttcggcgcgt acttctgcat caggtcgaag
       61 gccttcttgt accactcgct gcccggatag ttggcaccga gcacggcagc ggccttctgc
      121 gcctcggccg gaacgccaat cgccagattg ctttcaacca tgcgatagag cgcttcgggg
      181 gtgtggctgg tcgactgata gccttcgatc acgttgcgga agcgcaggtt ggcggcgagc
      241 cagcgacctg accgctgata gaaccgcccg atctccattt ccttgccagc gaggtgatcg
      301 ttgatcaggt cgatcttcag ccgcgcgtca gaggcgtagc gggtgttggg atagcgccgc
      361 accacctcgt tcatcgaatc gagcgcaagc cgggtgatct tctgatcacg gctgacgtcg
      421 ctgatctgct cgtaatagga aaggccgatc aggtaatagg catagggcgc atcgcggttg
      481 cccggatgga tcgacaggaa ccgctgtgcc gactggatcg ccttggtgta atcgcgcgcc
      541 acatagtacg agaaggcgct catcagctgg gcacggcggg cccagggcga atagggatgc
      601 tggcgctcca cctcgtcaaa cagcgcggcg gccagtttgg tatcgccgcg atcgagccgg
      661 tccttcgctg ccatgtaaag cgtatcgaca tcgcgcgcga caaaggccgt gtccttggcc
      721 tggttgccgc gcccggcgca cccggccgta accagcatgg ccgccgaagc agtggcgaaa
      781 agggcgagct tgagcggcga gcggctggtg ggcgttcctg gcatggcgcc agcctatagc
      841 cagagcctgc ctgaacgcca agtgaagttg cgcagcctat ccccgcccgc cgcaggggct
      901 tcagatattg gtgctggtcg ggatccggct gggcagcaga tagggatagg ccacccgccg
      961 cgcggcattg cgcgccgcga tccgctgttc cacgccctgc aggctcgcct ggaaccgcgc
     1021 cagcggtccc tccgggccgg tcacctgcag atcctggaac caggcgggat aggggaagtt
     1081 gcgcgactga taagtgccga gcgggcggta atggagcgac ccgagcaggt agagcgtttc
     1141 cagctgctgc agcgccgggg cgagcggcgg catatagccg agccattcgg cccgatcgtg
     1201 cccctgacgc tcgagcgggg catcggccca gccggcgcca gtcacggccg gggcaaattc
     1261 catcacgtcc ttctgcggga agttcacggc ggcatgctgg gcgctgcagg tgaacaggat
     1321 catcgtgcag gcatcgacca gtgcggccgc agtcgtgatc gtcgggaagc cggcgagctg
     1381 ccctgcgccc tgcagttcgg cgcaccaggc ggccagttcc ggatcgcccg ccacgtcggc
     1441 atcgctggcg tagtagagcc cgacatagcc caaaacccat tcgcggatcg cctgccagac
     1501 cagcagggca tcgtcgcgat agggatagtc gggcagcgcg ccggcatcat cgaccccgcg
     1561 ctccttcagg tcatgcggca gcatccggcg ggtaaagtcg aactccagcc gcgccgccac
     1621 cgcctgggcc tggctcgagg cgatcgtgcc ggcaaagata tggtcgatcg gtccgcccgg
     1681 cgtgatcagc gaggtggccg cggcgttgtt gatgaacagc gtgccctcgt aatgtggcac
     1741 cagcagcgcc caaagcggat gaacttcggc gaggtggcgg cgggtggcga gtgccagcgc
     1801 ctcgatcacc agatgcgtgt gggccaggtg cgcaatcagc tcgtggtaat tgccatcggc
     1861 cacctgaacg atgaacttgg cgatgtccca gccccattgc tcatcgggcc ggacgctggg
     1921 cgtgaagacc ggatcgaccg aaccatcctg cccgcactgg attgccaccg gcaccaggct
     1981 ggccccgccc ggcggcacgg cgaacagcgc cagcgggcga taggcgtact tggcctggcc
     2041 ctgataggac ccgggcacca gcttgctcag cgcggcatag tcgcacagat agatccggcc
     2101 ggcggccagc gcggtgttca gatcgtcgcc cgcaacaacc gcggcatagc gctcggcggt
     2161 cagggcaaag ttttcgggca gcgccgcgat ccgcttgatc agcgccgcgt tcggtccggc
     2221 gacgcgcagc cgggcgaatt cggcatcatc ctggaaggtc cagccgatcc ccggcagggt
     2281 cagcgtctgg aacagctcgc gatagcgctc caggtcgcgc ggggtgcggc tgcccagcgc
     2341 ggcatgttcg gccgcgctca ggctgaccac ctcgaacagg tgggccagct ggtcgcgcag
     2401 cgcagcgtca agcgccactg ccccggcggc gcccagcgcc agctcgatat cctcggcaat
     2461 ccggctccag atcgtcccgc ccgaagtgtt gtgagcgatc ttgctcctga tcccgtcgat
     2521 cgccagctcg atagcggcca gcgcggcgat ctcagcgccg atagccgcag gggcggcggg
     2581 gagcagtgcc tgttcggccc acagcttctt caccgccagg tggttcttga cgatcgccag
     2641 cagctcgtcc gccagcagga tcagccaggt cagcgtcggc tgctcgttcg cgggcggctg
     2701 accatcgagc aagggcgctc cggggagcga cggcaccgcc gtggtccagc gatagacgcc
     2761 ctgcgcagcc accagctggg cggcacgggc tgcctgctgc gccggcgtat cgttttgcgg
     2821 caggcttggg tgggcgggcg cctggtgcgg catggcaagt atctccccgg ttggtagttc
     2881 tgctgaattg gccaagtccc tgccacggcg gcgttaaaat cctcccaatc caagtatccg
     2941 gcttaagaaa tatttcaggg gctcccccta ttcttgctgg aatgaccagg ctggtggaca
     3001 agctgcgggg ctatttcacg ccccaggtgc ccgacgcgat ccgcgacgag ttcgtggtgc
     3061 gcggcgcggt gcagatgcag cacaattcgc gcctgctgtt ctttgccttg ttcctgacca
     3121 ccccgacc
//
LOCUS       DASUDV010000149         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1749885,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000149 DASUDV010000000
VERSION     DASUDV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749885"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFV30_03215"
     CDS             <1..568
                     /locus_tag="VFV30_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX4847130.1"
                     /translation="QFLDAENNRRDDRYGGSFENRCRIIHEVIDGIRAATGPQFQLGL
                     RLSPERFNIRLAEAKVLAEQVMTGGKLDYLDMSLWDCFKEPNEPDFAGTTLISQFTAL
                     PRGTTRLGVAGKIMDAAKAQACLDAGADFVLIGRGAILHHDFAARSIADPAFAAVPTP
                     VSAAHLRAEGLGEAFVSYMQSWKGFVEG"
     gene            complement(573..1697)
                     /locus_tag="VFV30_03220"
     CDS             complement(573..1697)
                     /locus_tag="VFV30_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4847131.1"
                     /translation="MTLRLGVVLPTYNERKNLRGMVDRLDKALAGIPWEVIIVDDNSP
                     DGTSDEARAIAQEDGRVRVIQRIGRRGLSSAAIEGMCATAAPVVAVMDADHQHDPELL
                     PGMLAAIESGEYDLSYASRFAEGASTEEWGRPDRVKASNLANKIANKVTGVELSDPMS
                     GYFMLKSEVLRADAHRLSGVGFKILLDILATVEQPLRIKEVPMNFAARLEGESKLDRT
                     VVFEFMVGLYDKWLGRWIPTRFALFGTIGGLGVVVHMAVLAMVLGLFGEAFAVAHYPK
                     EVAFIAGQTTAALVAMTFNFVLNNELTYADKRLRGFVPLLTGWVRFLITCAFGLLANI
                     GAATALVRLGFNPYPAAIVGIVLGSVWNFALSSKFVWGKY"
     gene            complement(1699..>2110)
                     /locus_tag="VFV30_03225"
     CDS             complement(1699..>2110)
                     /locus_tag="VFV30_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX4847132.1"
                     /translation="LSHMYVDNAAMQLVRSPGQFDVIVTGNLFGDILSDQASMCVGSI
                     GLLASASLSEGTLGLYEPIHGSAPDIAGKGLANPMATVLSAAMLLRHSLGLEDQAARI
                     EAAVARALADGVRGGDLGGSHSTVAIGDAILERL"
BASE COUNT          362 a          718 c          688 g          342 t
ORIGIN      
        1 ccagttcctc gatgccgaga acaaccggcg cgacgaccgc tatggcggca gcttcgagaa
       61 ccgctgccgg atcatccacg aggtgatcga cgggatccgc gccgccacgg ggccgcagtt
      121 ccagctgggc ctgcggctct cacccgaacg cttcaacatc cgtcttgccg aagcgaaggt
      181 gctggccgag caggtgatga ccggcggcaa gctcgactat ctcgacatgt cgctgtggga
      241 ctgtttcaag gaaccgaacg agccggactt tgccggaacc accctgatct cgcagttcac
      301 cgccctgccc cgcggcacca cccggctggg cgtggccggc aagatcatgg acgcggccaa
      361 ggcgcaggcc tgtctcgatg ccggggccga tttcgtgctg atcgggcgcg gggcgatcct
      421 gcaccacgat ttcgccgcgc gcagcattgc cgatccggcc ttcgccgccg ttcccacgcc
      481 ggtgtcggcc gcgcacctgc gcgccgaagg cctgggtgag gcctttgtca gctacatgca
      541 gagctggaag ggcttcgtgg agggctgagc cctcagtact tgccccagac gaacttgctc
      601 gacagcgcga agttccagac cgagccgagc acgatcccga cgatcgccgc cggatagggg
      661 ttgaacccca gccggaccag cgcggtcgcc gcgccgatgt tggcgagcag gccgaacgcg
      721 caggtgatca ggaagcggac ccagccggtc agcagcggca cgaacccgcg cagccgcttg
      781 tcggcatagg tcagttcgtt gttgagcacg aagttgaagg tcatcgccac cagcgcggcg
      841 gtggtctgcc cggcgatgaa ggccacttcc ttgggatagt gggccacggc aaaggcttcg
      901 ccgaacaggc ccagcaccat ggccagcacc gccatgtgga ccaccacgcc cagcccgccg
      961 atcgtgccga acagggcaaa gcgggtcggg atccagcggc ccagccactt gtcgtagagc
     1021 ccgaccatga attcgaacac gaccgtccgg tcgagcttcg attcgccttc cagccgggcg
     1081 gcgaagttca tcggcacttc cttgatccgc agcggctgtt ccaccgtggc caggatatcg
     1141 agcaggatct tgaagccgac gcccgagagg cggtgggcat cggcccgcag cacttcggac
     1201 ttcagcatga aatagccgct catcgggtcg ctcagctcaa cgccggtcac cttgttggcg
     1261 atcttgttgg cgaggttcga tgccttgacc cggtcgggcc ggccccattc ctcggtgctg
     1321 gccccttcgg caaagcggct ggcataggag aggtcgtatt ccccgctttc gatcgcggcg
     1381 agcatgccgg gcagcagttc gggatcgtgc tggtgatcgg cgtccatcac cgccaccacc
     1441 ggggcggcgg tggcgcacat cccctcgatc gcggcggagg acagcccgcg ccgcccgatc
     1501 cgctggatca cgcggacccg gccgtcttcc tgggcgatgg cgcgcgcctc gtcggacgtg
     1561 ccgtcggggc tgttgtcatc gacgatgatg acctcccacg ggatcccggc cagcgccttg
     1621 tcgagacgat cgaccatgcc gcgcaggttc ttgcgctcgt tataggtggg aaggacaacg
     1681 ccgagacgca gggtcatctt acagtctttc caaaattgcg tcgccgattg cgacagtgct
     1741 gtgactacca ccaagatcgc cgccgcgcac cccatcggcc agcgcacgtg caacggccgc
     1801 ctcgatccgc gcggcctgat cctccaggcc cagcgaatgg cgcagcagca tcgccgccga
     1861 cagcacggtg gccatcgggt tggccagccc cttgccggcg atgtccggcg cgctgccgtg
     1921 gatcggctcg taaagcccca gggtgccttc gctgagcgag gccgaggcca gcagcccgat
     1981 cgagccgacg cacatgctgg cctgatcgga caggatatcg ccaaacaggt tgccggtgac
     2041 gatcacgtcg aactggccgg ggctgcgcac cagctgcatc gcggcgttgt cgacatacat
     2101 gtggctgagc
//
LOCUS       DASUDV010000150         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_446426,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000150 DASUDV010000000
VERSION     DASUDV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446426"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /gene="ruvX"
                     /locus_tag="VFV30_03230"
     CDS             complement(<1..383)
                     /gene="ruvX"
                     /locus_tag="VFV30_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX4847133.1"
                     /translation="MADGPLITASALDYRAALPQAGALLGLDLGTQTIGTAFCDAGWR
                     FASPGKTLKRGKFGADREALAMLIAERGIKGLVLGLPLNMDGSEGPRSQSSRAYARNL
                     AAAFGLPVLLWDERWSTATAERDLIG"
     gene            complement(365..1480)
                     /locus_tag="VFV30_03235"
     CDS             complement(365..1480)
                     /locus_tag="VFV30_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX4847134.1"
                     /translation="MARKFLYVIAFCIVVFIAGRLALAFYPEQLTRMSFTPGGAFEPQ
                     PPLKANAYADPAMWLARPGMAGPNPVAWKPDGFAGNDPRVDAAVFFVHPTSYLKKTHW
                     NAPLDDADANRIATIVTRGNASVFNASGEIWAPRYRQATFGAFVSDEPAARQALDLAY
                     QDVAEAFDAFVAAQPAGRPIVLAGHSQGSFLLKRLIRERVAGTPLAPRIAAAYVVGWL
                     VDTDTELAAMGLPACAKADQAGCVVSFLSFTDQAETAMMRDAYRRFAGRSEGAADEAR
                     YLCSNPLTGGIGGTAAAAANPGGIVPDATFSAAKLAPGLAGAACAADGTLRIGEGPDM
                     GPFVLPGGNYHVYDYALYWLALRQDFARRVTAWQTAR"
     gene            complement(1484..2566)
                     /locus_tag="VFV30_03240"
     CDS             complement(1484..2566)
                     /locus_tag="VFV30_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4847135.1"
                     /translation="MARSPAKKASAPPADPAPVVHAGPTDITDTLIRTEAKKAAVWIG
                     MAALLALTVYLADALLVIFGGMVFAAMIDGGARLLARVLPIGRGLRITIVLLLTVAFL
                     AWVAVFAGSQIAAQAAQLPGIIEAQSLKVIAWLKGHGVQFDMQSAEGLIDKVVGGVGQ
                     ITNAVGGVIGGLTTAFLIAVMAIYFAVEPQLYRRGFAWLLPSANREHFEGTAMIMGRT
                     LRHLLAGRLLGMFIEGFATWAALALYGVPMAALLGLLTGLLAFLPNIGAPISGALMIM
                     VGFSGGVEMGIFCIVVYIVVQVVDGNIIVPMVAKKTADLAPALVLGAQLIMGALFGLL
                     GLMLADPLVAMLKVALERQSEKNESA"
     gene            complement(2566..>3090)
                     /locus_tag="VFV30_03245"
     CDS             complement(2566..>3090)
                     /locus_tag="VFV30_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX4847136.1"
                     /translation="IAVVNGQIVLNGKPVPQDVEPPLQLSADPKLTCSDRKQTYHCYN
                     DFMGYRTLLPSGREVLELPTLRETMPNGATYLIIKDRSDYLVDNYPETKVPEGHVFLM
                     GDNRNHSADSRVSILADGLGGPVPLSDVGGRAEFVTFSLDGSQTWNPLTWWKALRSGR
                     SWRSLRPARLTKDT"
BASE COUNT          511 a         1031 c         1103 g          445 t
ORIGIN      
        1 ccgatcaggt cgcgttcggc cgtggcggtg gaccagcgct cgtcccacag cagcaccggc
       61 aggccaaagg cggccgccag gttgcgggca taggcccggc ttgactggct gcgcggcccc
      121 tcgctgccgt ccatgttgag cggcaggccc aggaccaggc ccttgatgcc gcgttccgcg
      181 atcagcatgg caagcgcctc gcggtccgcg ccgaacttgc cgcgcttcag cgtcttgccg
      241 ggcgaggcaa agcgccagcc ggcatcgcaa aaggcggtgc cgatcgtctg ggtgcccagg
      301 tccagcccca gcagcgcccc ggcctggggc agggcggcgc ggtaatccag cgcgctggcc
      361 gtgatcagcg ggccgtctgc cacgcggtca ccctccgggc gaagtcctgg cgcagggcca
      421 gccagtagag cgcatagtca tagacgtggt aattgccgcc cggcagcacg aaggggccca
      481 tgtcgggccc ttcgccgatc cgcagcgtgc catcggcggc gcaggccgcg ccggccaggc
      541 ccggggccag cttcgcggcg gagaaggtgg catcgggcac gatcccgccc ggattggccg
      601 cggcagcggc ggtcccgccg atcccgcctg tcagcggatt ggagcagagg tagcgcgcct
      661 cgtccgccgc gccttcgcta cggccggcaa agcggcggta ggcatcgcgc atcatcgcgg
      721 tttcggcctg gtcggtgaag gacaggaaac tgacgacgca gccagcctgg tcggccttgg
      781 cgcaagccgg caggcccatg gcggccagtt cggtgtcggt atcgaccagc cagccgacca
      841 cataggcggc cgcgatccgc ggggccagcg gcgtgccggc gacccgctcg cggatcagcc
      901 gcttgagcag gaacgagccc tggctgtgcc cggccagcac gatcggccgg ccggcgggct
      961 gcgcggcgac gaaggcgtcg aaagcctcgg ccacgtcctg ataggccagg tcaagcgcct
     1021 ggcgggccgc gggctcgtcg ctgacgaagg ccccgaaggt cgcctggcgg taacgcgggg
     1081 cccagatctc gcccgaggcg ttgaacacgc tggcgttgcc gcgggtgacg atcgtggcaa
     1141 tgcggttggc atcggcatcg tcgagcggcg cgttccagtg ggtcttcttg aggtagctgg
     1201 tcgggtgaac gaagaacacc gcggcatcga cgcgcggatc gttgccggca aagccgtccg
     1261 gcttccacgc gacggggttg gggccggcca tgccggggcg ggccagccac atcgccggat
     1321 cggcataggc attggctttc agcggcggct gcggttcaaa cgcgccgccg ggggtgaagc
     1381 tcatccgggt cagctgctcg ggatagaagg ccagggcaag ccgcccggcg atgaacacca
     1441 cgatgcagaa ggcgatgaca tacaggaact tgcgggccaa ggttcaggcg ctttcgttct
     1501 tttccgattg ccgctccagc gcgaccttga gcatggcgac cagcggatcg gcgagcatca
     1561 ggccaagcag gccgaacagc gcgcccatga tcagctgggc ccccaggacc agcgccgggg
     1621 cgagatcggc ggtcttcttg gcgaccatcg gcacgatgat gttgccgtcc accacctgca
     1681 ccacgatgta gacgacgatg cagaagatcc ccatttcgac cccgccggaa aagccgacca
     1741 tgatcatcag ggccccggaa atcggcgcgc cgatgttggg gaggaaggcc agcaggccgg
     1801 tgagcaggcc cagcagggcg gccatcggca cgccgtagag cgccagcgcc gcccaggtgg
     1861 cgaagccttc gatgaacatg ccgagcaggc gcccggccag caggtgccgc agggtccggc
     1921 ccatgatcat cgccgtgcct tcgaaatgct cgcggttggc gctgggcagc agccaggcaa
     1981 agccgcgccg gtagagctgg ggttcgaccg cgaaatagat cgccatgacc gcgatcagga
     2041 acgcggtggt cagcccgccg atcaccccgc ccaccgcgtt ggtgatctgc ccgactccgc
     2101 cgacgacctt gtcgatcagc ccttcggcgc tctgcatgtc gaactggacg ccgtggcctt
     2161 tcagccatgc gatcaccttc agcgattgcg cctcgatgat cccgggcagc tgcgcggcct
     2221 gggcggcgat ctggctgccg gcgaagaccg cgacccaggc caggaaggca acggtcagca
     2281 gcaggacgat ggtgatgcgc aggccgcgcc cgatcggcag gacccgcgcc agcaaacgcg
     2341 cgccgccatc gatcatcgcg gcaaagacca tgccgccgaa aatgaccagc agggcatcgg
     2401 ccagatagac cgtcagcgcc agcagcgccg ccatgccgat ccacaccgcg gccttcttcg
     2461 cctcggtccg gatcagcgtg tcggtaatgt cggtcgggcc ggcgtgaacc accggcgccg
     2521 ggtcagcggg cggggcggag gccttcttgg cgggactgcg ggccatcacg tgtccttggt
     2581 cagacgggcg ggccgcaggc tgcgccagga ccggcccgaa cgcaaggcct tccaccaggt
     2641 cagcgggttc caggtctggc tgccgtcgag cgagaaggtg acgaattcgg cgcggccgcc
     2701 cacgtcggac agcggcaccg gcccgcccag gccgtcggcc aggatcgaga cccggctgtc
     2761 ggccgaatgg ttgcggttgt cgcccatcag gaaaacgtgg ccttccggca ccttggtctc
     2821 gggatagttg tcgaccaggt agtcgctgcg gtccttgatg atcagatagg tcgcgccgtt
     2881 cggcatggtt tcgcgcaggg tcggcagctc cagcacctcg cggcccgagg gcagaagggt
     2941 gcggtagccc atgaaatcgt tgtagcagtg gtaggtctgc ttgcgatcgc tgcaggtcag
     3001 cttgggatcg gcggaaagct ggagcggcgg ttcgacatcc tgcggaaccg gcttgccgtt
     3061 tagcacgatc tggccgttga ccacggcgat
//
LOCUS       DASUDV010000151         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_623241,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000151 DASUDV010000000
VERSION     DASUDV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623241"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFV30_03250"
     CDS             <1..565
                     /locus_tag="VFV30_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4847137.1"
                     /translation="GDVRRHSRYSVATLAFHEGIPGHVWQGEYANRLPLIRTLLAFNA
                     YSEGWALYAETLGDELGAYEGDPVGQLGYLQSIAFRACRLVVDTGLHAKRWTREKAVQ
                     WFAETNGSGLAEVAPEVDRYCSWPGQACGYKMGHTEILALRERAKRQLGAKYDLRDFN
                     DAVVTGGNVPLDVLGQNVDRYIAASRG"
     gene            664..1035
                     /locus_tag="VFV30_03255"
     CDS             664..1035
                     /locus_tag="VFV30_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX4847138.1"
                     /translation="MSIFGKIKDFVFGKKAEAATPPAAEAPAIPMAEAAPAPAPQPVD
                     IEAVLNAMPEAADLNWQSSIVDLMKLVGIDASFENRKELAHELGDADYSGSAEENIAL
                     HKAVMKALAENGGKVPANLLD"
     gene            complement(1111..1464)
                     /locus_tag="VFV30_03260"
     CDS             complement(1111..1464)
                     /locus_tag="VFV30_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847139.1"
                     /translation="MLDGILGQLGGNLDIAAIAEKAGIDPAMAEKAVAALGQSHAEPG
                     DTIEGAAAKTGLDVGQLGAIAQQLGGEGGLEKISSLIKDNPALSGVAGMLDRDGDGNP
                     LNDIAGIASSLFGKK"
     gene            complement(1530..1970)
                     /locus_tag="VFV30_03265"
     CDS             complement(1530..1970)
                     /locus_tag="VFV30_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847140.1"
                     /translation="MTDHPIRLFESDAAIRRVGEGLLARTLPKAEWTHEAHLAACAWL
                     VLERADIHPETALPNIIRSYNLAVGGVNDDTQGYHETLTQLYIIGVRHFLAGCAAEGL
                     LGKVNALLASDMAPRGWPFRFYSREVLFSAAARRGWVEPDRAAI"
     gene            complement(2013..3614)
                     /locus_tag="VFV30_03270"
     CDS             complement(2013..3614)
                     /locus_tag="VFV30_03270"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX4847141.1"
                     /translation="MTASLSHPLDQQGLATAATIHANLGTAALVEQALCRGEGRLTKD
                     GALLVDTGRFTGRSVKDKFVVRDAATESTINWGTINQPMGGEHWANLKADFLEALKGQ
                     DELFVADLFGGSQPEYRVNVRVITQLAWHNLFIRTLLVRPTADELASFVPEYTIINLP
                     SFKANPERHGCRSDTVIAVNFTEKLILIGNTEYSGEMKKGVFGLLNFLLPAQGVMPMH
                     CSANIGADGKSAIFFGLSGTGKTTLSADASRTLIGDDEHGWSDQAVFNFEGGCYAKMI
                     NLSAEGEPEIYATTKMFGTILENVAMDPATRELDFTDASKTENTRGAYPIEFIPNTSE
                     KNLGPPPSNIIFLTADAFGVLPPIARLTPDQAMYHFLSGYTAKVAGTEIGVTEPEATF
                     STCFGAAFMPRHPSVYGNLLKKRIAEGGAQCWLVNTGWSGGKATDPGIKRMPIKATRA
                     LLNAALDGSLNNAEFKKDPNFGFEVPVAVPGVDTKLLDPRGAWADPERYDQTAQELVA
                     RFVQNFAQFVDYVDQGVLDSAPKAA"
BASE COUNT          590 a         1167 c         1215 g          644 t
ORIGIN      
        1 gggcgatgtc agacgccaca gccgttattc ggtggcgacg ctggccttcc acgagggcat
       61 tcccggccac gtctggcagg gcgaatacgc caaccgcctg ccgctgatcc gcaccctgct
      121 ggccttcaac gcctattccg aaggctgggc gctctatgcc gaaaccctgg gtgacgagct
      181 gggcgcctat gaaggcgatc cggtcggcca gctgggctac cttcagtcga tcgccttccg
      241 ggcctgccgg ctggtggtgg acaccgggct tcacgccaag cgctggaccc gcgagaaggc
      301 ggtgcagtgg tttgccgaga cgaacggatc gggcctggcc gaagtggcgc cggaagtgga
      361 ccgctattgc tcctggccgg ggcaggcctg cggttacaag atgggccaca ccgaaatcct
      421 ggcgctgcgc gagcgggcca agcgccagct tggcgccaaa tacgatctgc gcgatttcaa
      481 cgacgcggtc gtgaccggcg gaaatgtccc gctcgacgtg ctggggcaga acgtggaccg
      541 ctatattgcg gcgagcaggg gataaattca ggtagggacc cgtggccttc gtgctagcgt
      601 gcgcaccgcg caggggaacc gcgcggcgtt gagtcgcaca tcagaacgct aaggagaatt
      661 cacatgtcca ttttcggcaa gatcaaggac ttcgtcttcg gcaagaaggc cgaagccgcc
      721 accccgccgg ccgccgaggc accggccatc ccgatggccg aggcggcccc cgccccggct
      781 ccgcaaccgg tcgatatcga agccgtgctg aacgcgatgc ccgaagcggc cgatctcaac
      841 tggcaaagct cgatcgtgga cctgatgaag ctggtcggga tcgacgccag ctttgaaaac
      901 cgcaaggaac tggcccacga actgggcgat gccgactatt ccggttcggc cgaggaaaac
      961 atcgccctgc acaaggcggt gatgaaggcc ctggccgaaa acggcggcaa ggttccggcc
     1021 aacctgctcg actgagcaag gcgccagcca cgaaaaaggg gctccccgcc acccggcgcg
     1081 gagccccttt ccgtgtgtgc ggcctgcgcc ttacttcttg ccgaacaggc tggaagcaat
     1141 gccggcaatg tcgttcagcg ggttgccgtc gccatcgcgg tccagcattc cggccacgcc
     1201 cgacagcgcc gggttgtcct tgatcaggct ggagatcttc tccagcccgc cttcgccgcc
     1261 cagctgctgc gcgatcgcac cgagctggcc gacgtcgagg ccggtcttgg ccgcggcgcc
     1321 ttcgatcgta tcgccgggtt cggcgtggct ctggcccagt gcggccacgg ccttttcggc
     1381 catcgccgga tcgatcccgg ccttctcggc aatcgccgcg atatcgagat ttccgcccag
     1441 ctggcccaga attccgtcaa gcattcccat gatttgcgac tcctatctgc gatccggcca
     1501 gtctggcgca ttgcccccga ccggccaagt caaatcgcgg cgcggtccgg ttcgacccag
     1561 ccccgccgcg ccgccgccga aaacagcact tcgcgtgagt agaacctgaa cggccagccg
     1621 cgcggggcca tgtcgctggc cagcagcgcg ttgaccttgc caagcaggcc ctccgccgcg
     1681 cacccggcca gaaagtgccg caccccgatg atgtagagct gggtcagggt ctcgtgatag
     1741 ccctgggtgt catcgttcac cccgccaacc gccaggttgt agctgcggat gatgttcggc
     1801 agcgcggttt cgggatggat gtcggcgcgt tccagcacca gccaggcgca ggcggcgagg
     1861 tgcgcttcgt gggtccattc ggccttgggc agggtgcgcg ccagcaggcc ttcgccgacg
     1921 cggcggatgg cagcgtcgct ttcgaacagg cggatcggat ggtcggtcat gaacggaaac
     1981 tccgggtcag gatcgacccg gagcgttccg ggtcaggcag ccttgggggc cgaatcgagc
     2041 acgccctgat cgacgtagtc gacgaactgg gcgaagtttt gcacgaaccg ggcgaccagt
     2101 tcctgtgcgg tctgatcata gcgctccgga tcggcccagg cgccgcgcgg atcgagcagc
     2161 ttggtgtcga cacccggcac ggccaccggc acttcgaacc cgaagttcgg gtccttcttg
     2221 aactcggcgt tgttcaggct gccgtcgagc gcggcgttga gcagcgcgcg ggtcgccttg
     2281 atcggcatgc gcttgatgcc cggatcggtg gccttgccgc cgctccagcc ggtgttgacc
     2341 agccagcact gcgccccgcc ttcagcaatc cgcttcttca gcaggttgcc atagacgctg
     2401 gggtggcgcg gcatgaaggc ggcgccgaag caggtgctga aggtcgcttc cggttcggtc
     2461 acgccgatct cggtgccggc gaccttggcg gtgtagcccg agaggaagtg gtacatcgcc
     2521 tggtccgggg tcagccgcgc gatcggcggc agcacgccaa aggcgtccgc ggtcaggaag
     2581 atgatgttgg acggcggcgg gccgaggttc ttctcgctgg tgttcgggat gaactcgatc
     2641 ggataggcgc cgcgggtgtt ctcggtcttg ctggcatcgg tgaagtccag ctcgcgggta
     2701 gccggatcca tcgccacgtt ctcaagaatc gtgccgaaca tcttggtcgt ggcgtagatt
     2761 tccggttcgc cctcggccga gaggttgatc atcttggcgt agcagccgcc ttcgaagttg
     2821 aacaccgcct ggtccgacca gccgtgctcg tcgtccccga tcagggtgcg gctggcatcg
     2881 gccgacaggg tggtcttgcc ggtgcccgac aggccgaaga agatcgcgct cttgccgtcg
     2941 gcgccgatgt tggcgctgca gtgcatcggc atcacgccct gcgcggggag caggaagttc
     3001 aagaggccga acacgccctt cttcatttcg cccgaatatt cggtgttgcc gatcaggatc
     3061 agcttctcgg taaagttgac cgcgatcacg gtgtcgctgc ggcagccgtg gcgttccggg
     3121 ttcgccttga agctgggcag gttgatgatg gtgtattccg gcacgaagct ggccagttcg
     3181 tcggccgtcg ggcggaccag cagggtgcgg atgaagaggt tgtgccaggc cagctgggtg
     3241 atgacgcgca cgttcacccg gtattcgggc tggctgccgc cgaacaggtc ggccacgaac
     3301 agttcgtcct ggcccttcag cgcctccagg aagtcggcct tcaggttggc ccagtgctca
     3361 ccgcccatcg gctggttgat cgtgccccag ttgatcgtgc tttcggtggc ggcatcgcgc
     3421 accacgaact tgtccttcac gctgcgcccc gtgaaccggc cggtatcgac cagcagcgcg
     3481 ccgtccttgg tcagccgtcc ctcgccgcgg cacagcgcct gttccaccag cgccgcagtg
     3541 cccagattgg catggatcgt ggcagccgtg gcaaggccct gctggtcgag cgggtgggaa
     3601 agcgaagcgg tcaagg
//
LOCUS       DASUDV010000152         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_976789,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000152 DASUDV010000000
VERSION     DASUDV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976789"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFV30_03275"
     CDS             complement(<1..576)
                     /locus_tag="VFV30_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4847142.1"
                     /translation="MVTVSTSNAAARAYHHGDLRSALVEAGLRALQTTTIADLSLRQL
                     AREVGVSATAVYRHFPDKQALMQALASEGIEQLGRYQQAAAESAGTNADAFGATGRAY
                     VRFALAHPALFRLIFGEGRDVGQSIFGENLAASLLRDKALKATGGDPARAQQLMIQAW
                     AVVHGLAMLMLDGQLPADEAMIDRVIDPETLF"
     gene            621..2054
                     /locus_tag="VFV30_03280"
     CDS             621..2054
                     /locus_tag="VFV30_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX4847143.1"
                     /translation="MASAVETAIRSVVTKGVTTVAKISRSIQDKDRAHPFLTGIHTPL
                     KDEHTITDLKVTGTIPAELSGRYVRIGPNPFNPDPRGHHWFIGDGMVHGVRLQDGKAL
                     WYRNRYIRSMDLESKGGPKAAPGPRRGRRDLINTNVIQHNGKVLAIVEAGSFPAELDE
                     NLETVAYTDFGGTLTAPFTAHPHEDPLTGELHAIVYDGMTQDTVWHVVVSKDGKVVRQ
                     EPIPVQDGPSIHECAITQKYVLVFDLPVTFSMKALIGGQQFPYSWNPEHKARVGLLPR
                     TGSADQIVWCEVDPCYLFHVANAYERADGVVVCDMSVHETMFAGGPDGPNGKPLGMER
                     WLIDPKGGKVRRTTLDSAPQEFHRPDERYFGQPYRYAWSLGLPGEDSAFLGDAPIYRY
                     DLETGERQAHDFGPGKVPGEFVFVPRRADAPEGDGWIMGYVIDTANETTDLVILDATD
                     LAKPPVAAVHIPHRIPPGFHGNWLPDA"
     gene            2140..2559
                     /locus_tag="VFV30_03285"
     CDS             2140..2559
                     /locus_tag="VFV30_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847144.1"
                     /translation="MDTERLAVIEAGLERAAETLGDVTAPVMAEFYRRHPDARASFAH
                     HMPANPASLEAEMVGNTLYYVMTWFEKPAEARIAFDSSVPHHRVALGVPPDWYRGFIE
                     AFLDVVEPAAVASDADRAVWQALRAGLVDLVERNRLA"
     gene            complement(2556..>2996)
                     /locus_tag="VFV30_03290"
     CDS             complement(2556..>2996)
                     /locus_tag="VFV30_03290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX4847145.1"
                     /translation="DHVVIGFPLWWGAEPAQLKGLLDRVLLPGWAFRYHKHDQMWDRL
                     LAGRSADLFVTMDTPPWFLRLFWSDPVMKRWNKQILEFAGFKPVRLFRIGPTRRGGAA
                     KNFGKWAARLEKAAASVGTLRRGSKDAARALRTDHETAQAERAS"
BASE COUNT          517 a         1030 c          978 g          471 t
ORIGIN      
        1 aaacagcgtt tcgggatcga tcacccggtc gatcatcgct tcgtccgctg gcaattggcc
       61 gtcgagcatc agcatggcca ggccgtggac cacggcccag gcctggatca tcagctgctg
      121 ggcgcgcgcc ggatcgccgc ccgtggcctt cagagccttg tcgcgcagca ggctggcggc
      181 caggttctcg ccaaagatcg actggccgac atcgcggcct tcgccaaaga tcagtcggaa
      241 cagggccggg tgggcgaggg cgaagcggac ataggcccgc ccggtcgctc caaaggcatc
      301 ggcgttggtc ccggccgatt cggcggcggc ctgctgatac cggcccagct gctcgatccc
      361 ttcgctggcg agggcctgca tcagggcctg cttgtcgggg aaatggcggt acacggcggt
      421 ggccgaaacc cccacctcgc gcgccagctg gcgcagcgaa aggtctgcaa tcgtggtggt
      481 ttgcagcgcc cgcaggccgg cctcgaccag cgccgaacgc aagtcgccat ggtggtaggc
      541 ccgcgccgcg gcattcgatg ttgacactgt taccataacc cctatgttta cggcataaac
      601 attaaaggga gattcgaccc atggcaagcg ccgtcgaaac cgcaatccgt tccgtcgtga
      661 ccaagggcgt caccaccgtc gccaagatca gccgctcgat ccaggacaag gaccgcgccc
      721 atccgttcct gaccggcatt cacacgccgc tgaaggacga gcacacgatc accgacctga
      781 aggtgacggg cacgatcccg gcggagctga gcgggcgcta tgtccggatc gggccgaacc
      841 cgttcaatcc cgatccgcgc gggcatcact ggttcatcgg tgacggcatg gtccacgggg
      901 tccggctgca ggacggcaag gcgctgtggt accgcaaccg ctacatccgc tcgatggatc
      961 tggaatcgaa gggcgggccc aaggccgctc cggggccgcg ccgcggccgg cgcgatctga
     1021 tcaacaccaa cgtcatccag cacaacggca aggtcctggc gattgtcgag gccggatcct
     1081 tcccggccga gctcgacgag aacctggaaa cggtggccta caccgatttc ggcggcaccc
     1141 tgaccgcccc gttcaccgcc cacccgcacg aggacccgct gaccggcgag ctccatgcga
     1201 tcgtctatga cgggatgacc caggacaccg tctggcacgt ggtcgtatcg aaggacggca
     1261 aggtcgtgcg gcaggaaccg atcccggtgc aggacggccc ttcgatccac gaatgcgcga
     1321 ttacgcagaa atacgtgctg gtctttgacc tgccggtgac cttctcgatg aaggcgctga
     1381 tcggcgggca gcagttcccc tattcgtgga acccggagca caaggcccgc gtcggcctgc
     1441 tgccgcgcac cggcagcgcc gaccagatcg tctggtgcga ggtcgatccc tgctatctct
     1501 tccacgtcgc caacgcctat gagcgcgccg acggggtggt ggtctgcgac atgtcggtcc
     1561 acgaaaccat gttcgccggc gggccggacg ggcccaacgg caagccgctg gggatggagc
     1621 gctggctgat cgatcccaag ggcggcaagg tgcggcggac cacgctcgac agcgcgccgc
     1681 aggaattcca ccgcccggac gagcgctatt tcggccagcc gtaccgctat gcctggtcgc
     1741 tgggtctgcc cggcgaggac agcgccttcc tgggcgatgc gccgatctac cgctatgatc
     1801 tggaaaccgg agagcgccag gcccacgact tcggccccgg caaggttccc ggcgaattcg
     1861 tcttcgtgcc gcgccgcgcc gatgcgccgg aaggggatgg ctggatcatg ggctatgtca
     1921 tcgatacggc caacgagacc accgatctgg tgatcctgga cgccaccgat ctggcgaagc
     1981 cgccggtcgc cgcggtgcac attccgcacc gcatcccgcc gggcttccac ggcaactggc
     2041 tgcccgacgc ctgatcccgc gccggatttg ccgcgcttga cttgacgcaa ttacggcccc
     2101 ttcctgttac tgccacagga caggacgcag ggaggggcca tggacaccga acgtctggca
     2161 gtgatcgagg cggggctgga acgtgcggcc gaaacgctgg gcgatgtgac cgcgccggtc
     2221 atggcggaat tctaccgccg ccatcccgat gcccgggcca gctttgccca ccacatgccg
     2281 gccaatcccg ccagcctgga agcggagatg gtcggcaaca cgctctatta cgtgatgacc
     2341 tggttcgaaa agccggccga agcgcggatc gcctttgaca gctcggtccc gcaccaccgc
     2401 gtcgcgctgg gcgtgccgcc ggactggtac cgcggcttta tcgaggcgtt ccttgacgtg
     2461 gtcgaacccg cagcggtcgc cagcgacgct gaccgcgcgg tctggcaggc gctgcgggcc
     2521 ggtctggtcg atctggtcga gcggaaccgg ctagcctagc tcgcccgctc ggcctgggcg
     2581 gtttcgtgat cggtgcgcag ggcccgggca gcgtccttgc tgccccgccg caaggtcccc
     2641 acgctggccg ccgccttttc caaccgggcg gcccatttgc cgaaattctt cgccgcgccg
     2701 ccgcgccggg tcgggccgat gcggaacagg cggaccggct tgaacccggc gaattccaga
     2761 atctgcttgt tccagcgctt catcaccgga tcgctccaga acaggcgcag gaaccacggc
     2821 ggggtgtcca tcgtgacgaa cagatcggcc gagcggccgg ccagcaggcg gtcccacatc
     2881 tggtcgtgct tgtgatagcg aaaggcccag ccgggcagca gcacccggtc cagcaggccc
     2941 ttcagctgcg ccggttccgc cccccaccac agcggaaagc cgatcaccac gtgatc
//
LOCUS       DASUDV010000153         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1153334,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000153 DASUDV010000000
VERSION     DASUDV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153334"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1650
                     /locus_tag="VFV30_03295"
     CDS             25..1650
                     /locus_tag="VFV30_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847146.1"
                     /translation="MTAPDRTIAAEPSASDMRLVIAASSAGTVFEWYDFFIYGALGAI
                     LSKTFFPTGNATLEMLLFWLVFAVGFGFRPLGAILFGYLGDKLGRKYTFLVTVTLMGV
                     ATAGVGLIPSAESIGLWAPAIVILLRILQGLALGGEYGGAAIYVAEHSPFHRRGYFTS
                     YIQASVVGGFVLSLVVVLGCKAVMSDETWAAWGWRVPFLLSILLLAISLWMRVKLSES
                     PVFQAMKEAGETAGNPFVESFTYPGNKKRIFIALFGIAAGLTVIWYTAMFSGLSFLQN
                     AMHVESTTAEIIVGISALIGMGFYIYFGKLSDRIGRKKPIVIGYALTLVLLFPTFWFI
                     GSVAKNPAQHAAIEWTIRGSDCSYDPFSTKQKTQCGKLMGDFAGAGVAYTLEKGDRLE
                     LVMGGSQVVALDGVDWNDAKARKAEIEARAGKTVFNFEKHKPGLGQSLLIIGALLVLM
                     GLSAATYGPVAALLAEMFPPKVRYSSMSIPYHIGTGYFGGFLPFIAGLIVARTGDPYS
                     GLWYTWFVVLMAMLVAWWGLPNGKPRDFSDDAA"
     gene            1640..2686
                     /gene="alr"
                     /locus_tag="VFV30_03300"
     CDS             1640..2686
                     /gene="alr"
                     /locus_tag="VFV30_03300"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX4847147.1"
                     /translation="MPPEAPPSPLRLTLDADALVANWRALDRLSGAARAGAAVKADAY
                     GLGAVRVVQVLSSAGCNDFFVAHWGEVAPLLDRIDPGRIAVLHGPLTAADAAYARAAG
                     VRPVINSLHQAALWQEAGGGLCDLMVDTGINRLGLPLEALSDPLIQGLTIDVLHSHLA
                     SAEEDVPLNALQQARWNAARGQIAHRRASLANSAGILLGADYHGDLTRPGIALYGGVP
                     CAALAGTIRQVARPQAAIMQVRTVAAGEGIGYNSTFVGDRDLRAGIVSLGYADGYLRC
                     WSGLGTLRTASGAPLPVLGRVSMDMTVIDLSAAPEAGEGDWVEADYALPEAAARTGLS
                     QYELLTLLGRRFAR"
     gene            2773..3327
                     /gene="phaR"
                     /locus_tag="VFV30_03305"
     CDS             2773..3327
                     /gene="phaR"
                     /locus_tag="VFV30_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX4847148.1"
                     /translation="MSDAKGETVIIKKYANRRLYNTKSSSYITLDHLAKMTREGVDFK
                     VVDAKSGADITHQILTQIIMEEESVRGEQMLPVNFLRQLIGMYGNSMQGLVPHYLEAS
                     MDQFRANQSKLREAFESSLGSNPLAKLAQQNLAMFKAAASAFVPGLADDKPAAKGKDQ
                     DDEIAALRKQMAEMQAKLDKLGKS"
BASE COUNT          559 a         1097 c         1153 g          641 t
ORIGIN      
        1 aaccgttcag gggagtatcg ccagatgacc gcacccgacc gcaccatcgc ggcagagccc
       61 agcgcatccg acatgcgcct ggtgatcgcc gcttcgtcgg ccggaaccgt gttcgaatgg
      121 tacgatttct tcatctacgg cgcgctcggc gcgatcctgt cgaagacctt cttcccgacc
      181 ggcaacgcca cgctggaaat gctgctgttc tggctggtct tcgcggtcgg ctttggcttc
      241 cgcccgctcg gcgcgatcct gttcggatac ctgggtgaca agctggggcg caagtacacc
      301 ttcctcgtca cggtcacgct gatgggggtg gcaacggcag gggtcggcct gatcccctca
      361 gccgagagca ttggcctgtg ggccccggcg atcgtgatcc tgctgcggat cctgcagggc
      421 ctggcgctgg gcggggaata cggcggcgcg gcgatctatg tggcggagca ttcgcccttc
      481 caccggcgcg gctatttcac cagctatatt caggccagcg tggtcggtgg cttcgtgctg
      541 agcctggtcg tcgtgctggg ctgcaaggcg gtgatgagcg atgagacctg ggccgcctgg
      601 ggctggcgcg tgccgttcct gctcagcatc ctgctgctgg cgatttcgct gtggatgcgg
      661 gtcaagctgt cggaaagccc ggttttccag gccatgaagg aagccggcga aacggccggc
      721 aacccctttg tcgaaagctt cacctatccc ggcaacaaga agcggatctt catcgccctg
      781 ttcggcatcg ccgccggcct gacggtgatc tggtacaccg cgatgttctc gggcctgtcg
      841 ttcctgcaga acgcgatgca cgtggaaagc acgacggccg agatcatcgt cggcatttcc
      901 gcgctgatcg ggatgggttt ctacatctat ttcggcaagc tgtcggaccg gatcggccgc
      961 aagaagccga tcgtgatcgg ctatgcgctg acgctggtcc tgctgttccc gaccttctgg
     1021 ttcatcggct cggtcgccaa gaacccggcc cagcatgcgg cgatcgaatg gaccattcgc
     1081 ggcagcgact gctcctacga tccgttctcg accaagcaga agacccagtg cggcaagctg
     1141 atgggcgatt ttgccggggc cggcgtcgcc tatacgctgg agaagggcga caggcttgaa
     1201 ctggtcatgg gcgggagcca ggttgtcgcg ctggacggcg tggactggaa cgatgccaag
     1261 gcgcgcaagg ccgagatcga ggcccgcgcc ggaaagaccg tgttcaactt cgaaaagcac
     1321 aagccgggcc ttggccagtc gctgctgatc atcggggcgc tgctggtgct gatggggctt
     1381 tccgcggcca cctatggccc ggtcgcggcc ctgctggccg agatgttccc gcccaaggtg
     1441 cgctacagct ccatgtcgat cccctatcac atcggcaccg gctatttcgg gggcttcctg
     1501 cccttcattg ccgggctgat cgtggcgcgg accggcgatc cctattcggg gctgtggtac
     1561 acctggtttg tggtgctgat ggccatgctg gtcgcttggt ggggtctgcc gaacggcaag
     1621 ccccgcgatt tcagtgacga tgccgcctga agcaccgcct tcgccgctcc gcctgaccct
     1681 tgatgcggac gcgctggttg ccaactggcg cgcgctggac cggctttccg gtgcggcgcg
     1741 ggccggtgcg gcggtcaagg ccgatgccta tggtctgggt gccgttcgcg ttgtgcaagt
     1801 tctgtccagc gcgggctgca acgacttctt cgtggcgcac tggggcgagg ttgccccgct
     1861 gctggatcgg atcgatccgg gccggatagc cgttctgcac ggtccgctga ccgcagccga
     1921 cgccgcctat gcccgcgccg ccggggtgcg cccggtaatc aactcgctgc accaggccgc
     1981 gctgtggcaa gaggctggcg gcggcctgtg cgatctgatg gtcgataccg ggatcaaccg
     2041 tctcggcctg ccgctggagg cgctgtccga tccgctgatc cagggcctga ccatcgatgt
     2101 gctgcattcg caccttgcct cggcggaaga ggacgtgccg ctcaacgcgc tgcaacaggc
     2161 acgctggaac gccgcgcgcg ggcagatcgc ccaccgccgg gccagcctgg ccaacagcgc
     2221 cgggatcctg cttggggccg actatcacgg ggatctgacc cggcccggga ttgcccttta
     2281 tggcggcgtg ccctgtgcgg cgctggccgg cacgatccgc caggtcgccc gtccgcaggc
     2341 cgcgatcatg caggttcgca ccgtcgccgc gggagagggg atcgggtaca attccacttt
     2401 cgtgggcgat cgcgacttgc gggccgggat cgtcagcctg ggctatgccg atggctacct
     2461 gcgctgctgg tcggggctgg gcacgctgcg gaccgcctcc ggggcaccgc tgccggtgct
     2521 cggccgggtc tcgatggaca tgactgtgat cgacctttcc gccgcgcccg aagcgggcga
     2581 gggggactgg gtggaggccg actatgccct gccggaggcg gccgcccgga ccgggctgtc
     2641 ccaatatgaa ctcctgaccc tgctcggccg ccgttttgcg cggtgatgct gcgcagcaag
     2701 gatttgttgc aatgccgttg ttgcaggtgc taactgcacc caaaggggcc tttccctgcg
     2761 gggttttgga tcatgagcga cgccaagggc gaaaccgtaa tcatcaagaa gtacgccaac
     2821 cggcggctct acaacaccaa gtcgtcaagc tatatcacgc ttgatcacct ggcgaagatg
     2881 acccgcgaag gggtggattt caaagtcgtc gacgcgaaaa gcggggccga cattacccac
     2941 cagatcctga cccagatcat catggaagag gaaagcgtgc gcggtgagca gatgctcccg
     3001 gtcaatttcc tgcgccaact gatcggcatg tacggcaatt cgatgcaggg gctggtgccg
     3061 cactacctcg aagcgtcgat ggaccagttc cgcgccaacc agtcgaagct gcgcgaggcc
     3121 ttcgaatcga gcctgggcag caacccgctg gccaagctgg cccagcagaa cctggccatg
     3181 ttcaaggctg cggcctcggc gtttgtccct ggtctcgcgg acgacaagcc cgccgccaag
     3241 ggcaaggacc aggacgacga gattgccgcc ctgcgcaagc agatggccga aatgcaggcc
     3301 aagctggaca agctggggaa gagctaggcc gctttctgcg gaaagatcgg catgcccggg
     3361 cgctcgaatg cgcgtccctt gccgaccttc cattcaaccg gatcgaaccc gctggggaag
     3421 gtgaattgcc ccagcttcag cacggccgcc
//
LOCUS       DASUDV010000154         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_181486,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000154 DASUDV010000000
VERSION     DASUDV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181486"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..521)
                     /locus_tag="VFV30_03310"
                     /pseudo
     CDS             complement(36..521)
                     /locus_tag="VFV30_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(518..901)
                     /locus_tag="VFV30_03315"
     CDS             complement(518..901)
                     /locus_tag="VFV30_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4847149.1"
                     /translation="MAKRRVTSGSPLEPVIGFCRAVRTGKQIMVAGTAPIEPDGSTTP
                     GDAAAQMERCCAIVAKAIADLDGSIDDVVRTVIYLTDRADFESVARIHGKWFGVARPA
                     CTTLIVSGFVRPEWKVEIEAEVMEA"
     gene            complement(904..2403)
                     /locus_tag="VFV30_03320"
     CDS             complement(904..2403)
                     /locus_tag="VFV30_03320"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX4847150.1"
                     /translation="MRFVDHFIAGGTAGLAANRKGAVMDPNNGGQQAEVVLGSADALE
                     RAVAAAQAAQPGWAAMNPQRRARVMFEFKRLIERDMDELAALLSSEHGKVIADSKGDI
                     QRGLEVVEFCCGLPHVLKGEYTQGAGPGIDVYSMRQPIGIGAGITPFNFPAMIPLWMG
                     AMAIAAGNAFILKPSERDPSVPNRLAALFLEAGLPEGVFQVVHGDTEMVDLILDHPAI
                     GAVSFVGSSDIAHYVYERGVHAGKRIQAMGGAKNHGIVMPDADLDQVVGDLCGAAFGS
                     AGERCMALPVVVPVGEDTAERLKAKLIPAIEALKVGISTDPDAQYGPVVTAMHKANIE
                     RWIQTAVDEGSELVIDGRGFKMQGHEEGYFLGPTLLDRVTPEMTSYHNEIFGPVLQIV
                     RAKDFEEALTLPSKHQYGNGVAIFTRNGHAAREFASRVNVGMVGINVPIPVPVAYHSF
                     GGWKRSGFGDTDQYGMEGLRFWTKVKKITARWPDGGGDGSNAFIIPTMG"
     gene            2498..3397
                     /locus_tag="VFV30_03325"
     CDS             2498..3397
                     /locus_tag="VFV30_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4847151.1"
                     /translation="MQATDWNDFQAFLAIARAGQLARAATLMGVDATTMGRRLRRLEA
                     RLGATLFEQTREGQVLTEAGEALLVRVEAMAQAASGIAEGAGTAGALSGTLRISTSEG
                     FGSWFLARQVPAFCAAHPGLTLDIVASSGFLSLSKREADIAVMLSRPKAGPVIARKLA
                     DYALRLYAAPAYLEAQGCPQRPADLAQGHRLIGYIPDLLYDPSLRYLDEIHPGLAASL
                     RSSSINAQHRLLAAGAGVGVLPCFMGDTDPALVAVLPERRIVRSFWLVTHRDTQNLAR
                     IKAGKDWLLDSVLRQRDLLLPPG"
BASE COUNT          553 a         1159 c         1120 g          636 t
ORIGIN      
        1 ccggtgcgga ccatggcgac atagatgtcg ttctagcccg cgccggaaat gaactgcttg
       61 gtgccgttga gaacgtaagt atcgccatcg agccgcgcgc tggttttgag cgcggccgcg
      121 tccgagcccg agcccggctc ggtcaggcag taggaggcaa tcttttccat gctgacgaga
      181 cccggcagga agcggtgctt cagttcctgc gaaccgaagg tgtcgatcat ccaggtcgcc
      241 atgttgtgga tcgagatgta ggcggacgta gccgggcagc cataggccat ggcttccatg
      301 atcagcgcgg cttcgagccg gccaaggccg atcccgccgt tctcttccga aacatagatc
      361 gccccgaagc ccagttcgcc cgcggccttc cagacatcga ccggatagtg gcttttttca
      421 tcccattcgg cggcgaaggg cgtgatccta tcggcagtga acttgcgcgc catgtcctgg
      481 atcgcaagct ggtcttcggt gagggaaaac tggtcggtca tgcttccatt acttccgctt
      541 cgatttcgac tttccattcc ggacgcacga agcctgaaac gatcagggtg gtgcaggcag
      601 gccgtgcaac gccgaaccac ttaccgtgga tccgcgcaac gctttcgaag tcggcccggt
      661 cggtgagata gatcaccgtg cgcaccacat cgtcaatgct tccatccaga tcggcaatcg
      721 ccttggcgac aatcgcgcag caccgttcca tctgcgcggc ggcatcgccg ggcgtggtcg
      781 aaccatccgg ctcgatcggc gcggttccgg ccaccatgat ctgcttgccg gtgcgtaccg
      841 cgcggcagaa cccgatcacc ggttcaagcg gcgagccgct tgtgacgcgt cgcttggcca
      901 caatcacccc atcgtcggga tgatgaaggc gttgctgcca tcgccgccgc catcgggcca
      961 gcgggcggtg atcttcttga ccttggtcca gaagcgcagg ccttccatgc cgtactggtc
     1021 ggtatcgcca aagcccgaac gcttccaccc gccgaagctg tgataggcca caggcaccgg
     1081 gatcggcacg ttgatcccga ccatgccgac gttcacgcgg ctcgcgaatt cgcgcgcggc
     1141 gtggccgttg cgggtgaaga tcgccacgcc gttgccatac tgatgcttgc tgggcagggt
     1201 gagcgcctct tcaaagtcct tggcgcgcac gatctgcagc acgggcccga agatttcgtt
     1261 gtggtagctg gtcatctcgg gggtcacccg gtcgagcagc gtgggcccca ggaaatagcc
     1321 ttcctcgtgg ccctgcatct tgaagccgcg cccatcgatc acgagttcgc tgccttcatc
     1381 cacggcggtc tgaatccagc gttcgatatt ggccttgtgc atcgcggtga ctacgggccc
     1441 gtactgcgcg tcgggatcgg tcgaaatgcc gaccttgagc gcttcgatcg ccgggatcag
     1501 cttggccttg aggcgttcgg cggtgtcttc gcccaccggc accaccaccg gcagcgccat
     1561 gcagcgctcg ccggccgaac cgaaggccgc gccgcacagg tcgccgacca cctggtcaag
     1621 atcggcatcg ggcatgacga tgccgtggtt cttggctccg cccatcgcct ggatccgctt
     1681 gcccgcgtgc acgccgcgtt cgtagacgta gtgggcaatg tcggacgagc cgacgaagct
     1741 gaccgcgccg atcgccgggt gatcaaggat caggtcgacc atttccgtgt cgccgtggac
     1801 cacctggaac accccttcgg gcaggccggc ttcaaggaac agcgcggcca ggcgattggg
     1861 aaccgaggga tcgcgctcgg aaggcttgag gatgaaggca ttgccagcgg cgatcgccat
     1921 cgcgcccatc cacagcggga tcatcgccgg gaagttgaac ggggtgatgc ctgcgccgat
     1981 cccgatcggc tggcgcatcg aatagacatc gatgccagga ccggcgccct gggtgtattc
     2041 gcccttcagc acgtgcggca ggccgcagca gaattcgacc acttcgagcc cgcgctggat
     2101 atcgcccttg ctgtcggcaa tgaccttgcc gtgttcgctg gaaagcagcg cggccagctc
     2161 gtccatgtcg cgttcgatca ggcgcttgaa ttcgaacatc acccgcgcgc ggcgctgcgg
     2221 gttcatcgcc gcccagccgg gctgcgcggc ctgggccgcg gccacagcgc gttccagcgc
     2281 atcggcgctg cccagcacca cttcggcctg ctggccgccg ttgttgggat ccatcaccgc
     2341 gcccttgcgg tttgcagcca ggccggcggt gccgccagcg atgaaatgat cgacaaaacg
     2401 catgggagtg ccttcctctg ggtgttcgct ttgatcctgc cctgcacctg ctttcgcttg
     2461 caggcaagtc gcaattctgc gaccattgcc tgcaattatg caggcaactg actggaacga
     2521 ctttcaggcc ttcctggcca tcgcccgcgc cggccagctg gcccgcgccg ccacgttgat
     2581 gggggtcgat gcgaccacca tggggcggcg gctgcggcgg ctcgaggcgc ggctgggcgc
     2641 caccctgttc gagcagaccc gcgaaggcca ggtgctgacc gaagcgggcg aggcactgct
     2701 ggtgcgggtg gaggccatgg cccaggccgc cagcgggatc gccgagggtg ccggcaccgc
     2761 cggggcgctt tcgggaacgc tgaggatcag cacctcggaa gggttcggat cgtggttcct
     2821 ggcgcgccag gtgccggcct tctgcgccgc gcaccccggc ctcacgctcg atatcgttgc
     2881 ttccagcggg ttcctcagcc tctcgaagcg cgaggcggat atcgcggtca tgctctcgcg
     2941 gcccaaggcc gggccggtga tcgcccgcaa gctggctgac tatgcgcttc ggctctatgc
     3001 cgcgcccgcc tatcttgagg cgcagggctg cccgcagcgc ccggcggacc tcgcccaggg
     3061 gcaccggctg atcggctata ttcccgatct gctttacgat ccctcgctgc gctatctcga
     3121 cgagatccac cctggccttg ctgcctcgct gcgctcctca agcatcaatg cccagcaccg
     3181 cctgctggct gcgggcgcgg gggtgggggt gctgccctgc ttcatgggcg ataccgatcc
     3241 ggcactggtg gcggtcctgc ccgagcggcg gatcgtccgc tcgttctggc tggtgacgca
     3301 ccgcgacacg cagaacctgg cgcggatcaa ggccggcaag gactggctgc tcgattcggt
     3361 tctgcgccag cgcgatctgc tgcttccacc cggctagaag cgcgtctaca aggcagccat
     3421 ggccgatcag cccgccccga tcgaaccggc cgcgccgcag ggcggcga
//
LOCUS       DASUDV010000155         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_601191,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000155 DASUDV010000000
VERSION     DASUDV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601191"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="VFV30_03330"
     CDS             <1..1695
                     /locus_tag="VFV30_03330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4847152.1"
                     /translation="KTVQLWAIPAKGGRARQVTSLKDGITDFALAPDGKRAVVVAEVD
                     PTPHPRYTGKAKDAPRSSPPIVIDRFNFRQDGRDWIEDGTAQLHLVDLKNGSATQLTK
                     GAFDVSAPSWSPDGSLIAYASWRDAAADRVGDSDVFVIAPQVDATPRRISPSNNADAD
                     PISSGERPAWSPDSRRLAWTTQAEPKWIYYSPPQLSVADLASGTVKEVARIDRFTYAP
                     RWSSDGSRILAMIEQDRAMWLASIDPDADKVSYLSNGPVAATGFALGAGGRIALLEGT
                     DTTIDRLRKLGEDDSVLADHNPWLAQRQLGEVREVAYPSADGTPIHGLVTLPPPGVAP
                     AGKKPPLMVRVHGGPVSQYDHSFSPDAQIYAAQGYVVLTVNPRGSSGRGFDFARAIYA
                     DWGNKDVADVSAGISWVLDNGMADPDRIAVGGWSYGGILSNYLVASDPRIKAAVSGAG
                     VSNVFGLWGVDQYIREYEQELGTPWANADTFTRLSYPFFKADRIKAPTLFLCAEEDHN
                     VPCAGAQQMYQALKSTGVPTRLVVYPGETHGLTAPSHVRDRIERSLAWYERYLNGK"
     gene            complement(1682..1939)
                     /locus_tag="VFV30_03335"
     CDS             complement(1682..1939)
                     /locus_tag="VFV30_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847153.1"
                     /translation="MDWQARLGDFLQHDWVRWYLLPGLGAALAALLAWQGERRRKRRS
                     DPDAVGLLDWTNVTFWASFAALVLFGAAIRGYLSSGVPIFR"
BASE COUNT          370 a          672 c          640 g          338 t
ORIGIN      
        1 aagaccgtgc agctctgggc catcccggcc aagggcgggc gggccaggca ggttaccagc
       61 ctcaaagacg ggattaccga tttcgcgctt gcgcccgatg gcaagcgcgc tgtggttgtt
      121 gccgaagtcg atcccacgcc gcaccccagg tacaccggca aggccaagga cgccccccgc
      181 tcaagcccgc cgatcgtgat tgatcgtttc aactttcgtc aggacggacg cgactggatc
      241 gaagacggca ccgcccagct tcatctggtc gatctcaaga acggctcggc aacgcagttg
      301 accaagggcg ccttcgacgt ttccgcgccc tcgtggtctc cggatggcag cctgatcgcc
      361 tatgcctctt ggcgcgatgc cgctgccgac cgggttggtg acagcgatgt cttcgtgatc
      421 gcgccacagg tggatgccac gccgcgccgg atcagcccta gcaacaacgc cgatgccgat
      481 ccgatcagtt cgggcgaacg gcccgcctgg tcgccagatt cgcggcgcct ggcctggacc
      541 acccaggccg aacccaaatg gatctactat tcgccccccc agctttcggt tgccgatctg
      601 gccagcggca cggttaagga agtggcgcgg atcgaccggt ttacctatgc cccgcgctgg
      661 tcgagcgatg gcagccggat tctggccatg atcgaacagg atcgcgcgat gtggttggcc
      721 agcatcgatc cagatgccga caaggtcagc tatctgagca acggcccagt ggccgcgacc
      781 ggttttgcgc tgggcgcggg cggtcggatc gcgctgctgg aaggcaccga tacgaccatc
      841 gaccgtctgc gcaaactggg cgaagacgat agcgtactgg ccgatcacaa cccctggctg
      901 gcccagcgac aattgggcga agtgcgcgag gtggcctacc ccagcgcgga tggaacgccg
      961 atccacgggc tggttaccct gcccccgccc ggagttgctc cggcaggcaa gaagccgccg
     1021 ctgatggtgc gggtccatgg cgggccggtt tcgcagtacg atcactcgtt cagtcccgat
     1081 gcgcagatct atgccgcgca gggctatgtc gtgctgacag tcaacccgcg cggctcttcg
     1141 gggcgcggtt tcgatttcgc ccgggcgatc tatgccgatt ggggcaacaa ggacgttgcc
     1201 gatgtcagcg cggggatcag ctgggtgctc gacaacggca tggccgatcc tgaccgcatc
     1261 gcggtgggcg gctggtcata tggcggcatc ctctccaact acctcgttgc cagcgatcca
     1321 cgtatcaagg ccgcggtcag cggggccggc gtttccaacg tattcgggct ttggggggtc
     1381 gatcagtaca tccgcgaata cgagcaggaa ctgggcacgc cctgggccaa tgccgatacc
     1441 tttacccggc tcagctatcc cttcttcaag gccgacagga tcaaggcgcc aaccctattc
     1501 ctgtgcgccg aggaggatca caacgtacca tgcgctggcg cacagcagat gtaccaggcg
     1561 ctcaaatcca ccggcgttcc aacccggctg gtggtctatc cgggtgaaac ccatggacta
     1621 accgccccga gccacgtgcg cgaccggatc gaacgatcgc tggcctggta cgagcgctac
     1681 ctcaacggaa aataggcacg ccgctgctca gatagccgcg aatcgccgcg ccaaacagca
     1741 ccagcgcggc aaagctggcc cagaaggtga cgttggtcca gtcgagcagc ccgaccgcat
     1801 caggatcgga gcggcgcttg cggcggcgtt cgccctgcca ggccagcagg gccgccaggg
     1861 cggcacccag cccgggcagc aggtaccagc gcacccaatc gtgctgcagg aaatcgccaa
     1921 ggcgcgcttg ccagtccatc ccggcccgct agagcaatcg ggtgcaacct gtccagaagc
     1981 ccctccttgc gcttggcctc aggctggctt cggccgtggt
//
LOCUS       DASUDV010000156         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_513018,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000156 DASUDV010000000
VERSION     DASUDV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513018"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFV30_03340"
     CDS             <1..670
                     /locus_tag="VFV30_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX4847154.1"
                     /translation="TAYHEAGHALVSVNEPASDPIHKATIIPRGGALGMVVRLPERDR
                     YSYHRDQMHAQLAVAMGGRVAEEIIFGHDKVSSGASSDIQQATSLARAMVTRWGMSEK
                     LGPLQYEETQEGYLGYGGSARTMQSAETNKLIDAEIRALVEGGHKRATKILKDHEDKL
                     HLLAKALLEYETLTGEEIDQLIKDGKIDRPVDPSGPASVPPVKGSAVPKAGKRFGGAA
                     AQGA"
     gene            complement(696..1775)
                     /locus_tag="VFV30_03345"
     CDS             complement(696..1775)
                     /locus_tag="VFV30_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX4847155.1"
                     /translation="MSGGGEIIGDIAQGALLSRAAEPDHGEGQDDGHTHERACLNCGT
                     ALVGAHCHACGQAAHVHRTLGAFFHDLLHGVFHFEGKIWRTFPLLVWRPGKLTREYID
                     GRRASYVSPIALFLFVVFLSFAVFGSLGSPVNFDTPPKTELANGMAEAEKEALAVQAD
                     LERKLADARAGKGGDVAALEGEVAKAKQAAAGIRAARTGELPEQITEGDFGSSAATAE
                     QLNEAWQHAKENPELIAYKLQTSAYKYAWLVIPLSVPFVWLLFPFSRRFHLYDHTVFV
                     TYSVSFQLLLLALIGAVTVWAGTGGPLSGLLLLYSPVHMYRQARETYGLSRFGAWWRT
                     WFLAGFAVLVLALFASGLAVYAAAG"
     gene            1832..2236
                     /locus_tag="VFV30_03350"
     CDS             1832..2236
                     /locus_tag="VFV30_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847156.1"
                     /translation="MRCEIAGRSRMHRYALALAASTVLAAPLLAAPGDMSVATFLAKA
                     DALRAKGPMALMSSDYKLLQSEGKGAGDAYRARLRAERAAGRPSSCPPKDVKVSSDKL
                     IAHLRTYPEAVRPRVSMKQAMADYFIRTYPCR"
     gene            complement(2309..>2417)
                     /locus_tag="VFV30_03355"
     CDS             complement(2309..>2417)
                     /locus_tag="VFV30_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX4847157.1"
                     /translation="DEIDEMGSLRQSADALRITAAAPVRTTSIGGDYDG"
BASE COUNT          424 a          833 c          812 g          348 t
ORIGIN      
        1 gaccgcctat cacgaggccg gccacgcgct ggtttcggtg aacgagccgg ccagcgatcc
       61 gatccacaag gccacgatca tcccgcgcgg cggcgcgctg ggcatggtgg tgcgcctgcc
      121 cgagcgggac cgttattcct accaccgtga ccagatgcac gcgcagctgg ccgtggcgat
      181 gggcggccgc gtggcggaag agatcatctt cggccacgac aaggtctcgt ccggcgcctc
      241 cagcgatatc cagcaggcca cctcgctggc ccgcgcgatg gtgacccgct ggggcatgtc
      301 ggaaaagctg gggcccctgc agtacgagga aacccaggag ggctacctcg gctatggcgg
      361 ctcggcccgg accatgcagt cggccgaaac caacaagctg atcgacgccg aaatccgcgc
      421 cctggtggaa ggcgggcaca agcgcgccac caagatcctg aaggaccacg aggacaagct
      481 gcacctcctg gccaaggccc tgctcgaata cgagacgctg accggcgaag agatcgatca
      541 gctgatcaag gacggcaaga tcgaccgccc ggtcgatccg tccggcccgg catcggtgcc
      601 cccggtcaag ggatcggccg tacccaaggc cggcaagcgg ttcggcggag cggcggcgca
      661 gggggcctga gccccctgcc cgccagccgg cgggctcagc cggcggcggc gtaaaccgcc
      721 agtccggagg cgaacagggc cagcaccagc acggcaaagc cggccaggaa ccaggtgcgc
      781 caccacgcgc cgaaccggct gaggccatag gtctcgcgcg cctggcggta catgtgcacc
      841 ggagaataga gcagcagcag gcccgagagc ggaccaccgg tgcccgccca caccgtcacc
      901 gcgccgatca gcgccagcag caacagctgg aaactgaccg aataggtgac gaaaaccgtg
      961 tggtcgtaga ggtgaaagcg gcggctgaac gggaacagca gccagacgaa cggaaccgac
     1021 aacgggatca ccagccaggc gtatttgtag gcgctggtct gcagtttgta ggcgatcagt
     1081 tcggggtttt ccttggcgtg ctgccaggcc tcgttgagct gttcggccgt ggcggccgag
     1141 ctgccgaaat cgccctcggt gatctgctcg ggcagctctc cggtccgcgc ggcgcgaatt
     1201 ccagccgcgg cctgcttggc cttggccact tccccttcca gcgcggccac atcgccgccc
     1261 ttgccggcgc gggcatcggc cagcttgcgc tccagatcgg cctgaaccgc cagcgcctcc
     1321 ttctcggcct cggccatgcc gttcgcgagc tcggtcttgg gcggggtatc gaaattgacc
     1381 gggctgccca gcgatccgaa caccgcaaag ctcaggaaca cgacgaacag gaacagcgcg
     1441 atcggactga cgtagctggc ccggcggcca tcgatgtatt cgcgggtcag cttgcccggg
     1501 cgccagacca gcagcgggaa cgtgcgccag atcttgcctt cgaagtggaa caccccgtgc
     1561 agcagatcgt gaaagaacgc gcccagcgtg cggtggacgt gcgcggcctg gccgcaggca
     1621 tggcagtgcg ccccgaccag cgcggtgccg cagttgaggc aggcgcgttc atgggtgtgg
     1681 ccatcgtcct ggccctcgcc gtggtccggt tcggccgcgc gcgaaagaag cgcgccttgc
     1741 gcaatatcgc caatgatttc gccgccgccg ctcatggggt ccctcccgct tcgtcctgtt
     1801 catggtcttg caagccggac gcggtcaagc catgcgttgc gaaatcgccg ggagatcacg
     1861 aatgcaccgc tatgcccttg cccttgccgc cagcacggtc ctggccgccc cgctgctcgc
     1921 cgcgccgggc gacatgagcg tcgccacttt cctggccaag gccgatgccc tgcgcgccaa
     1981 ggggccgatg gcgctgatgt cgtcggatta caaattgctg caaagcgagg gcaagggcgc
     2041 gggcgatgcc tatcgcgcgc ggctgagggc cgagcgcgcc gccggccgcc cgtccagctg
     2101 cccgcccaag gacgtcaagg tcagctccga caagctgatc gcccacctgc gcacctatcc
     2161 cgaagctgtc cgcccgcggg tgtcgatgaa gcaggccatg gcggactatt tcatccgcac
     2221 ctatccctgc cggtaaaggc gcaaacgaag aggcccgcgg tcaccaggac cgcgggcctt
     2281 tcgtttggcc tcagcgaacg cccggcgctt agccgtcgta atcgccgccg atcgaggtgg
     2341 tgcgcaccgg cgccgcggcg gtgatccgca gggcgtcggc cgactggcgg agcgagccca
     2401 tttcgtcgat ctcgtcg
//
LOCUS       DASUDV010000157         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2103297,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000157 DASUDV010000000
VERSION     DASUDV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103297"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1095)
                     /gene="purM"
                     /locus_tag="VFV30_03360"
     CDS             complement(4..1095)
                     /gene="purM"
                     /locus_tag="VFV30_03360"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX4847158.1"
                     /translation="MSEKSYSYEQAGVSIAAGNALVKAIGPLVKATARPGADAEIGGF
                     GGFFDPKAAGFKDPLLVAANDGVGTKVKLAIDHDRHDTIGQDLVAMCVNDLIVQGAEP
                     LFFLDYFATGKLETGVAERVVASIAEGCKLAGCALIGGETAEMPGMYAAGDYDLAGFC
                     VGAVERGEQMTGDKVASGDVLLGLASSGVHSNGYSLVRRLAADKGWKLDRPALFDPER
                     LLIDHLIEPTRIYVKSLLPVIRSGRIHALAHITGGGLLENIPRVLPEGLHARVDAGAW
                     EQPRLMAFLQAQGNIEPAEMARTFNCGIGMVLAVSADDAASVQADLEAAGETVFVIGA
                     IEPGARGCTVQGSAEDWSARDAWEASHLG"
     gene            1168..2448
                     /locus_tag="VFV30_03365"
     CDS             1168..2448
                     /locus_tag="VFV30_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX4847159.1"
                     /translation="MAVAPLPSLLRSWRPGPVATLLGGLSLALVLALALTGPERLWAQ
                     IEGDRGIVPVASTSDIEIMGIEVNTSGNTAEEARLNGWQEAARKAWAKAGGPAMSDGQ
                     LQGMVSAVVIEREQIGPKRYVATLSVVFDRARAGQYIGGKVGGNAHSAPLLVIPVLHS
                     GGTAQVFEVKGPWQRAWAEFRAGNSPIDYVRPSGGGGDSLLITAGQPSRRSRAWWRNV
                     LDQFGAADVIMPEARLERQWPGGPIRGTFTARYGPDNTFLESFTLTANDEAGLPKMLA
                     DAVVRLDQIYARALADGRLKPDTTLNVRPGIDPGLAALIAAGQAPEAAPTTAPTATPT
                     AAPTATPTVAPVVETRTFTVQFASPDAAAVDAALGAVRGAPGVKGASTTSLAVGGTSV
                     MRVTYEGDLDTLAAALRARGWKVTVGNNALSIRR"
     gene            2450..3064
                     /locus_tag="VFV30_03370"
     CDS             2450..3064
                     /locus_tag="VFV30_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX4847160.1"
                     /translation="MGQIALPLAAGSPGPSRIVLGSANQPAVEAMAAAGSWPFRTAIL
                     VGPPRSGKSLIARWFAETGQGEAIDDADRADETELFHRWNRAQEAGTALLLVAGGGPG
                     EWQIALPDLRSRLGAALHLSIGTPDDAMLGDLILIHAEQRGLALGPDAIAYLVPRCER
                     SHIGVERLVAAIDRISLERKVAPTQGVWREALEELLGPSEPRLL"
     gene            3094..3807
                     /gene="epsC"
                     /locus_tag="VFV30_03375"
     CDS             3094..3807
                     /gene="epsC"
                     /locus_tag="VFV30_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX4847161.1"
                     /translation="MLEDLKRYLDSVVARDPSPRSRWEVLLYPGVWALGLHRVAHWLF
                     RTELFFAARLVNHISRFLTAIDIHPGARIGENFFIDHGFVVIGETAEIGNNVTMYQGS
                     TLGGTNPTNGVGGKRHPTIGDNVIISLGAAILGPIHVGAGARIGANAVVTKDVPEGAT
                     MVGIPAKPTLVEVKPETQNFVPYGTPCSERFDPATQKLELLQCEMQVLQKRLAELLDE
                     RDATAAKQSAARKRKAKSA"
     gene            3804..4151
                     /locus_tag="VFV30_03380"
     CDS             3804..4151
                     /locus_tag="VFV30_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX4847162.1"
                     /translation="MSATVTPFPLSAPRPAQVGFERDELQRILDLYGRMVAAGQWRDY
                     AMDFGKDAAVFSAFRRTAERPSARIEKRPALRSKQGMWALIGEAGQVIKRGHELPGVL
                     FPLERRLLKLVED"
     gene            complement(4154..>4613)
                     /locus_tag="VFV30_03385"
     CDS             complement(4154..>4613)
                     /locus_tag="VFV30_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX4847163.1"
                     /translation="ILPAAIGLSAWLWRAHANLHADGREALNYTPGWAVGSLALPFVN
                     LVVPMQAMRELWNRSHGEEQWGARQTVAAVSSWWTCFVCGALLHTVLMAVVLLDLGTD
                     FAFITPPGVNMFFLFFATALLGVSAWYLYRIIGAITRAQHTATHVGDTFA"
BASE COUNT          718 a         1586 c         1624 g          685 t
ORIGIN      
        1 cgctcagcca aggtggctgg cttcccaggc atcgcgggcg gaccaatcct cggcgctgcc
       61 ctgcacggtg cagccgcgcg cgccgggttc gatggcgccg atcacgaaga ccgtttcgcc
      121 cgccgcttcc agatcggcct ggaccgaagc ggcatcgtcg gcgctgaccg ccaggaccat
      181 gccgatcccg cagttgaagg tgcgggccat ctcggccggt tcgatgttcc cctgggcctg
      241 aaggaaggcc atcagccgcg gctgttccca ggccccggca tcgacccggg cgtggagccc
      301 ttcgggcagg acgcggggga tgttttcgag aaggccgccg ccggtgatgt gggccagggc
      361 gtggatccgg ccgctgcgga tcaccggcag caggctcttc acgtaaatcc gggtcggctc
      421 gatcaggtgg tcgatcagca gccgttcggg atcgaacagg gcggggcgat ccagcttcca
      481 gcccttgtcc gcggccagcc gccggaccag cgaatagccg ttggagtgga cgcccgagga
      541 ggcgaggccg agcagcacgt cgccgctcgc caccttgtcc ccggtcatct gctcaccgcg
      601 ttccacggcg ccgacgcaaa agccggccag atcatagtcg cccgcggcgt acatccccgg
      661 catttcggcc gtttccccgc cgatcagcgc gcagccggcc agcttgcagc cctcggcaat
      721 gctggcgacg acccgctcgg ccacgccggt ttccagcttg ccggtggcga agtagtccaa
      781 gaagaacagc ggctccgcgc cctggacgat cagatcgttg acgcacatcg cgaccaggtc
      841 ctgcccgatc gtgtcgtgcc ggtcatggtc gatcgccagc ttgaccttgg tgccgacccc
      901 gtcgttggcg gcgaccagca gcggatcctt gaagcccgcg gccttgggat cgaagaagcc
      961 gccgaagccg ccgatttccg catcggcgcc gggccgcgcg gtggctttca ccagcgggcc
     1021 gatcgccttg accagggcgt ttccggccgc gatcgaaacg ccggcctgtt cgtagctgta
     1081 ggacttttca gacatcgagt gcgcgcctag cgctttcgcg cttggatttc catgtgtgat
     1141 tgggcaaaag gccagcggtt ttccccgatg gcagtcgcac ctctcccttc cttgctgcgc
     1201 tcatggcgcc ccggccctgt cgccacgctg cttggcggcc tgtcgctggc gttggtgctg
     1261 gcgcttgcgc tgactgggcc ggaacggctt tgggcccaga tcgagggcga ccgcgggatc
     1321 gtccccgtgg ccagcacctc cgacatcgag atcatgggga tcgaggtcaa caccagcggc
     1381 aacacggccg aagaggcccg cctcaacggc tggcaggagg ccgcgcgcaa ggcctgggcc
     1441 aaggccggcg gcccggcgat gagcgacggg cagctgcaag gcatggtctc ggccgtggtg
     1501 atcgagcgcg agcagatcgg cccgaagcgc tatgtcgcga cgctgagcgt ggtgttcgac
     1561 cgcgcccgcg ccgggcagta catcggcggg aaagtgggcg gcaacgccca ttccgcgccg
     1621 ctgctggtga tcccggtgct ccattccggc ggcaccgcgc aggtgttcga ggtcaagggc
     1681 ccgtggcagc gcgcctgggc cgagttccgc gccggcaaca gcccgatcga ctatgtccgc
     1741 ccctctggcg gcgggggcga ttcgctgctg atcaccgccg gccagcccag ccggcgcagc
     1801 cgtgcctggt ggcgcaacgt gctggaccag ttcggcgcgg ccgacgtgat catgcccgaa
     1861 gcccggctgg aacggcagtg gcccggcgga ccgatccgcg gcaccttcac cgcgcgctac
     1921 ggcccggaca acaccttcct ggaaagcttc accctgaccg ccaacgacga ggccggcctg
     1981 cccaagatgc tggccgacgc ggtggtccgg ctggaccaga tctatgcccg cgcgctggcc
     2041 gacgggcggc tcaagcccga tacaacgctg aacgtccggc ccgggatcga ccccggcctg
     2101 gcggcgctga tcgcggcggg ccaggccccc gaagcggccc cgaccaccgc gccgaccgcg
     2161 acgccgaccg ccgcgccgac cgctacgccg accgtcgccc cggtggtcga aacccgcacc
     2221 ttcacggtcc agttcgcctc gcccgatgcc gcggcggtcg atgccgcgct gggcgcggtg
     2281 cgcggcgcgc cgggggtgaa gggcgcctcg accaccagtc tcgccgtcgg cgggacctcg
     2341 gtgatgcgcg tgacctacga aggcgatctc gataccctgg ccgcggcgct gcgtgcgcgc
     2401 ggctggaagg tgacggtggg caacaacgca ctgtcgatca ggcgctgaca tgggccagat
     2461 cgcgctgccg cttgccgccg gttcgcccgg gccctcgcga attgtcctgg gatcggccaa
     2521 ccagccggcg gtggaggcca tggccgcggc cggatcctgg ccgttccgca ccgcgatcct
     2581 ggtcggcccg ccgcgttcgg gcaagtcgct gatcgcgcgc tggtttgccg aaaccgggca
     2641 gggcgaagcg atcgacgatg ccgaccgggc cgacgagacc gagctgttcc accgctggaa
     2701 ccgcgcgcag gaagccggca cggcgctgtt gctggtggcg ggcggcggtc cgggggaatg
     2761 gcagatcgcc ttgccggacc tgcgatcccg gctgggcgcg gcgctgcacc tgtcgatcgg
     2821 cacgcccgac gatgccatgc tgggggacct gatcctgatc cacgccgaac agcgcgggct
     2881 ggcgctgggg cccgatgcga tcgcctatct tgtcccgcgc tgcgaacgtt cccatatcgg
     2941 ggttgagcgg ctggtcgcgg cgattgaccg gatcagcctg gagcggaagg tcgcccccac
     3001 ccagggggtc tggcgggaag cactggagga actgctgggg ccaagcgagc cgcgcttgct
     3061 ttagcccggc ggacatggga ctatgaggga accatgctgg aagacctgaa gcgctatctc
     3121 gattccgtgg ttgcccgcga cccgagcccg cgttcgcgct gggaagtgct gctctatccc
     3181 ggcgtctggg cgctgggcct gcaccgggtg gcgcactggc tgttccgcac cgaactgttc
     3241 ttcgcggccc gcctggtcaa ccacatcagc cggttcctga ccgcgatcga cattcaccct
     3301 ggcgcgcgga tcggtgagaa tttctttatc gaccacggct tcgtggtgat cggcgaaacc
     3361 gccgaaatcg gcaacaacgt gaccatgtac cagggcagca ctctgggcgg caccaacccc
     3421 accaacggcg tgggcggcaa gcgtcacccg acgatcggcg acaatgtgat catcagcctt
     3481 ggcgcggcga tcctgggccc gatccatgtc ggcgcgggcg cgcggatcgg ggccaacgcg
     3541 gtggtgacca aggacgtgcc cgaaggcgcg acgatggtcg gcatcccggc caagccgacc
     3601 ctggtggagg tcaagcccga gacgcagaac ttcgtgccct atggtacccc gtgcagcgaa
     3661 cggttcgatc cggccacgca gaagctggaa ctgctccagt gcgaaatgca ggtcctgcaa
     3721 aagcgcctgg ccgagctgct ggacgagcgt gacgccacgg cggccaagca aagcgcggca
     3781 cgcaagcgca aggcgaagtc ggcttgagcg caaccgtcac gccattcccg ctctccgcgc
     3841 ccaggccggc gcaggtcggg ttcgaacggg acgagttgca acgcattctg gatctttacg
     3901 ggcggatggt cgcggccggg cagtggcgcg actacgcgat ggatttcggc aaggacgcgg
     3961 ccgtgttctc ggcctttcgc cgcaccgcgg aacgcccctc ggcgcggatc gagaagcgcc
     4021 ccgcgctgcg gtccaagcag gggatgtggg cgctgatcgg cgaggccggg caagtgatca
     4081 agcgcgggca cgagctgccc ggcgtgctgt tcccgctcga acggcggctg ctcaagctgg
     4141 tcgaggatta gggtcaggca aaggtgtcgc ccacgtgagt ggcggtgtgc tgggcgcggg
     4201 tgatcgcgcc gatgatccgg tacaggtacc acgccgacac acccagcagc gccgtggcga
     4261 agaacaggaa gaacatgttc acccccggcg gggtgatgaa ggcgaaatcg gtgcccaggt
     4321 ccagcaggac caccgccatc agcacggtgt gcagcagcgc cccgcagacg aagcaggtcc
     4381 accacgagga gaccgcggcc accgtctggc gggcgcccca ttgctcttcc ccgtggctgc
     4441 ggttccacag ctcgcgcatc gcctgcatcg gcacgaccag gttgacgaag gggagggcca
     4501 ggctgcccac ggcccagccg ggcgtgtagt tcagcgcctc gcgcccgtcg gcatgcaggt
     4561 tggcgtgggc gcgccacagc caggcggaca ggccgatcgc ggcgggcagg atg
//
LOCUS       DASUDV010000158         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_866443,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000158 DASUDV010000000
VERSION     DASUDV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866443"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VFV30_03390"
     CDS             complement(<1..1129)
                     /locus_tag="VFV30_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4847164.1"
                     /translation="MSRIFHSSVIAVALASGWVTPAQASAAKEPSAAEQFASLQAQLN
                     QLNQRVDALEGQLERERARADAAEARATKAAADAEAAKAEAARLAAASKPAEPPVAVA
                     WKGAPELSHKDGWSFKPRGRLHLDGGSVSSPGAFTSPTLGGNLRVRRARLGAEGTMPG
                     GFGYKAELDFANGNVAMADFFLSYSPRKTPLTIRIGNFETLNGLEQVTSSNNISILER
                     ASFNDAFLNSRRLGAAVAVKSKSDDLRAEVAVFAAHSMDSSLDNDGWIGAARLVYAPK
                     ALGGQLHLGVNYQYREFGSNISGGTSVGSNMPSVNQLARYRARPGTQLTDVRFVDTGN
                     FAAQGDSILGLEGAAIFKGIYFAGEAQWVNTRAYSAGDIATG"
     gene            1328..2242
                     /locus_tag="VFV30_03395"
     CDS             1328..2242
                     /locus_tag="VFV30_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4847165.1"
                     /translation="MTISFKDRVAIVTGAGGGLGRAYALELAKRGAKVVVNDLGGSRD
                     GTGHSDAALKVVEEIKAMGGEAMSNGGSVTEYEQMVEMVAKAKEAWGGVHILINNAGI
                     LRDKSFAKMDMADWKLVVDVHLNGSANCTKAVWDLMREQTYGRILNTASSTGLFGNFG
                     QANYGAAKLGLAGFTKTLALEGAKYNVRVNTLAPVAGTRMTEDLGMPEMLFNALKPEN
                     VAPMALYLVSEDAPTNMICGAGAGAYHAAYITLTPGVALPADERTPEGIAAHWDQIID
                     RAGEIVPQSGGEQSQVVMAALAKIGGLG"
BASE COUNT          387 a          757 c          786 g          363 t
ORIGIN      
        1 tcccggtcgc gatatcgccg gcggaatagg cccgggtgtt cacccactgg gcttcgccgg
       61 caaaatagat ccccttgaag atcgccgcgc cttccaggcc caggatgctg tcgccctggg
      121 ccgcgaaatt gccggtgtcg acaaagcgga cgtcggtcag ctgggtgccc ggccgggcgc
      181 gatagcgggc cagctggttg accgacggca tgttcgaacc gaccgaggtg ccgcccgaaa
      241 tgttcgatcc gaactcgcgg tactggtaat tgacgcccag gtgcaactgg ccgcccagcg
      301 ccttgggcgc atagaccagc cgcgccgcgc cgatccagcc atcgttgtcg aggctgctgt
      361 ccatcgagtg ggcggcaaag acggcaactt ctgcgcgcag gtcgtcgctt ttgctcttca
      421 ccgccacggc cgcgcccagc cggcgcgagt tcaggaaggc gtcgttgaag ctggcacgct
      481 caaggatcga aatgttgttg gagctggtca cctgctccag cccgttgagc gtttcaaagt
      541 tgccgatgcg gatcgtcagc ggggtcttgc gcgggctgta ggacaggaag aagtcggcca
      601 tggccacgtt gccgttggcg aaatccagct cggccttgta gccgaacccg cccggcatgg
      661 tgccttcagc gcccaggcgg gcgcggcgca cgcgcaggtt gccgcccagc gtgggcgagg
      721 tgaaggcgcc cgggctggag accgaaccgc cgtcgaggtg caggcgtccg cgcggcttga
      781 acgaccagcc gtccttgtgg gacagttccg gcgcgccctt ccaggccacg gccaccggcg
      841 gttcggccgg cttgctggca gcggcaagcc gcgcggcttc ggccttggcg gcttcggcat
      901 cggcggcggc cttggtggcg cgggcttccg cagcgtcggc gcgggcgcgt tcgcgctcca
      961 gctggccttc cagggcatcg acccgctggt tcagctggtt gagctgcgcc tgcagcgacg
     1021 cgaactgctc ggccgcgctc ggctccttgg cggcggaggc ctgtgccggg gtcacccagc
     1081 cggaagccag cgcgacggca atgacggacg aatggaagat gcggctcacg gtttttgcct
     1141 cccttgggcg gaatatgacg attcggggtc gatcctagga ccgttcggtg acacttattt
     1201 gacaactcgg ttgcccctgc cgctacggca cggggcttcg tcgctgccac ggttgaaacg
     1261 ccgcatcgtt catgccagtg acgccgcgct tccccagcgg gattcaccca taaacggagc
     1321 agtgccaatg accatctcgt tcaaggatcg cgttgcgatc gtcaccggcg ccggcggcgg
     1381 cctgggccgc gcctacgcgc tcgaactcgc caagcgcggg gccaaggttg tcgtcaacga
     1441 tctgggcggc agccgcgatg gcaccggcca ttccgacgcc gcgctcaagg tcgtcgaaga
     1501 gatcaaggcg atgggcggcg aagccatgtc gaacggcggc tcggtcaccg aatacgagca
     1561 gatggtcgaa atggttgcca aggcgaagga agcctggggc ggcgtgcaca tcctgatcaa
     1621 caacgccggg atcctgcgcg acaagagctt cgccaagatg gacatggccg actggaagct
     1681 ggtcgtcgat gttcacctga acggcagcgc caactgcacc aaggcggtgt gggacctgat
     1741 gcgcgagcag acctatggcc gcatcctcaa caccgccagc tcgaccggcc tgttcggcaa
     1801 cttcggccag gccaactacg gcgcggccaa gctgggcctg gccggcttta ccaagacgct
     1861 ggcgctggaa ggcgcgaagt acaacgtgcg cgtcaacacc ctggccccgg tggccggcac
     1921 ccgcatgacc gaagacctcg gcatgccgga aatgctgttc aacgcgctga agccggaaaa
     1981 cgtcgccccg atggcgctct atctggtcag cgaagacgcg ccgaccaaca tgatctgcgg
     2041 cgcgggcgcg ggtgcctatc acgccgccta catcaccctg accccgggcg tggccctgcc
     2101 ggcggacgag cgtacgcccg aaggcatcgc cgcccactgg gaccagatca tcgaccgcgc
     2161 cggcgaaatc gtcccccagt cgggcggcga acagagccag gtggtgatgg ccgcgctggc
     2221 caagatcggc ggcctgggct gatcacagcc gctggccgtc tggccggaac gaaggggcct
     2281 ggacgcgagt ccg
//
LOCUS       DASUDV010000159         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_999021,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000159 DASUDV010000000
VERSION     DASUDV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999021"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VFV30_03400"
     CDS             complement(<1..1044)
                     /locus_tag="VFV30_03400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4847166.1"
                     /translation="MRKRTIALGTAAALLLAGGGWYASQDRETRDLIANLPTDRDVLM
                     WKQDQRDAAFRALDRLPALAEAREILPSATALPLPQGKPLTIPGIDAYMAGQRSAGIV
                     ILQDGKVRFERYGLGFDAKGRWTSFSVAKSFTSTLVGAAIQDGYIKSLDDKVSQYIPG
                     LRGSAYDDVSVRQLLTMSSGVKWNEDYEDPKADVAQFNNAKPEAGMDITVSYMRTLPR
                     AHPPGTVWHYNTGETNLIGVLVSSATKKPLSQYLQEKIWQPAGMEAKATWLLGATGHE
                     IAGCCMQAAVRDYARFGLFVLANGQAGGKQIVPADWFAQAIVKQKDIGQPGHGYGFQW
                     WTYDDGSVAAQGIF"
     gene            1134..1991
                     /locus_tag="VFV30_03405"
     CDS             1134..1991
                     /locus_tag="VFV30_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847167.1"
                     /translation="MKRSNVLLSLLGLIALAKASAAPSQTMEPGERHQVLLEYHNEFS
                     NSDGSEGSSQGRQAITVRLIAKRDGGQELEFALPDSATEQDRLREWQLPARIFVAADG
                     RGTLLNTSELEVRRNRFLAAAKLTQAACGKWVFTWNAFKIECDPQSALEIAETYRLQP
                     QGPLTDGAPFTQRDAVGSAVLACSPVDGGGQVCSVTLPVDAAKLRREKAEADVVVGEI
                     NGHPVTLVDALKAYERVEVEGTIETVLAVDADGSVTKRTTIVRVKLREPDGKTETSTS
                     TSVLTRTRL"
     gene            complement(1988..2878)
                     /locus_tag="VFV30_03410"
     CDS             complement(1988..2878)
                     /locus_tag="VFV30_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX4847168.1"
                     /translation="MNEEPTPERLVSGLLKLLEVTPDGEDRFAGRRKRGGVGRVFGGE
                     VIAQALNAAEATVPDDRPAHSLHAYFLRGGSEEHPSDYKVDRDFDGGSFSNRRVVASQ
                     QGRPILNLTASFQRREEGLHHIDAVMPDVPPPEELEPEVEVRRRYAEHARPEARHHFL
                     SPRPVEMRAVEGRHWMNPSPAPPRSHSWFKTVAPLPDDPRVHRAILAYASDMTLLGTC
                     ALPHGLSWARGEVVSASLDHAVWFHEPFRADEWLLYATDSPWSGGGRGFNRGRIFTRD
                     GRLVASVAQEGMIRRVTPKG"
     gene            complement(2954..>3647)
                     /locus_tag="VFV30_03415"
     CDS             complement(2954..>3647)
                     /locus_tag="VFV30_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX4847169.1"
                     /translation="IERFARIFTPGSLVHFASVDDHVEDRQTVLYLEDLASQAGMEAR
                     FVAIDKIGVNAAGQLVDDQQTLIGALFKLYPWEALMRSVYAPQLAASSALFLEPMWKA
                     ILSNKAILPLLWERHRGHRNLLPAAFEGTPGADAIAAGPHAVKPFFSREGADIELFDG
                     TRRTRGPAEGYGAEGRIVQAFAPLAQSGSNHAVIGSWVVGDDAVAMSVREDAGPITRD
                     LARFLPHALVGD"
BASE COUNT          608 a         1166 c         1246 g          627 t
ORIGIN      
        1 gaagatgccc tgcgccgcga ccgagccgtc gtcataggtc caccactgaa agccatagcc
       61 gtggcccggc tggccgatgt ccttctgctt gacgatcgcc tgggcgaacc agtcggccgg
      121 cacgatctgc ttgcccccgg cctggccgtt cgccagcacg aacaggccaa agcgggcata
      181 gtcgcggacc gcggcctgca tgcagcagcc ggcgatctcg tgcccggtgg cgccgagcag
      241 ccaggtggcc ttggcttcca tcccggccgg ttgccagatc ttctcctgca gatactgcga
      301 aagcggtttc ttggtggcgc tggacaccag caccccgatc aggttggttt cgccggtgtt
      361 gtaatgccag accgtgcccg gcggatgggc gcggggcagc gtgcgcatat agctgacggt
      421 gatgtccatg cccgcttcgg gcttggcgtt gttgaactgg gcaacatcgg ccttgggatc
      481 ttcgtaatcc tcgttccact tcacgcccga gctcatcgtc aggagctggc ggaccgagac
      541 atcgtcatag gccgatccgc gcaggccagg gatgtactgg ctgaccttgt cgtccaggct
      601 cttgatatag ccgtcctgga tcgccgcccc gaccagagtg gaggtgaacg acttggcgac
      661 cgagaagctg gtccagcggc ccttggcgtc gaaaccgagg ccgtagcgtt cgaaccggac
      721 cttgccgtcc tgcaggatca cgatcccggc cgagcgctgc ccggccatgt atgcgtcgat
      781 cccgggaatg gtcagcggct tgccctgcgg cagcggcagc gcggtggcgg aaggaaggat
      841 ctcccgcgcc tcggccagcg ccggcaggcg gtccagcgcc cggaacgcgg cgtcgcgctg
      901 atcctgcttc cacatcagga catcgcggtc ggtcggcagg ttggcgatca gatcgcgggt
      961 ttcgcggtcc tggctggcat accagccgcc accggccagc agcagggccg ccgccgtgcc
     1021 cagtgcgatc gtgcgcttgc gcattctgta cctccccttg tctctcacca gcctagcagc
     1081 gcggctactt gcttcaactt tgatgattca agcaatttgt gtagattgga aaaatgaaac
     1141 gctcgaatgt gttgctctct ttgctcggct tgattgcgct ggccaaggca agtgccgccc
     1201 cctcgcagac catggaaccc ggcgagcggc atcaggtgct gctggaatac cataacgagt
     1261 tcagcaacag cgacgggagc gaaggatcgt cccagggtcg gcaggcaatt accgtccgtc
     1321 tgatcgccaa gcgtgacggc gggcaggagc ttgaatttgc tctgccggat agcgcgaccg
     1381 aacaagaccg gctgcgcgaa tggcaattgc cggcgcgcat tttcgttgcg gcggacggcc
     1441 ggggcacgct gctcaacacg tccgaactgg aagttcgacg gaaccggttt ctggccgcag
     1501 ccaagctcac gcaagcggct tgcggcaaat gggtattcac ctggaacgcc ttcaagatcg
     1561 agtgcgatcc gcaatctgca ctggagattg ccgaaactta ccgattgcag ccgcaaggtc
     1621 cactaaccga tggtgctccg ttcacgcagc gtgatgctgt tggcagcgcg gtgctcgctt
     1681 gcagtccagt cgatggcggc ggacaagtct gctcagtgac cttgccggtc gatgctgcca
     1741 agctgcggcg cgaaaaggcc gaagccgatg tggtcgttgg cgagatcaat gggcacccgg
     1801 tcacgctcgt cgacgcgctg aaggcatatg agcgagttga agtggagggc acgatagaaa
     1861 ccgtgctggc ggttgatgcc gatggcagcg tgaccaagcg gaccacgatc gtccgcgtca
     1921 agctgcgcga acctgacgga aaaaccgaga cttcgaccag tacttcggtt ctgacccgga
     1981 cgaggctcta acccttcggc gtcacccttc ggatcatgcc ctcttgcgcg acgctggcga
     2041 ccagccgtcc gtcgcgggta aagatccggc cccggttgaa gccgcggccg ccgccgctcc
     2101 acgggctgtc ggtggcatag agcagccact cgtcggcccg gaacggctcg tggaaccaga
     2161 ccgcgtggtc caggctggcg ctgaccactt cgccgcgggc ccacgacagg ccgtggggca
     2221 gggcgcaggt gcccagcagg gtcatgtcgc tggcataggc caggatcgca cggtggacgc
     2281 gcggatcgtc gggcaggggg gcgacagtct tgaaccagga atggctgcgc ggcggggccg
     2341 ggctggggtt catccagtgc cgtccttcca cggcgcgcat ttcgaccggg cgagggctga
     2401 ggaaatggtg ccgcgcctcg gggcgggcgt gctcggcata gcggcggcgc acctccacct
     2461 cgggctccag ctcctcgggc ggggggacat cgggcatcac cgcatcgatg tgatgcaggc
     2521 cttcctcgcg gcgctggaaa ctggcggtca ggttcaggat cggccggccc tgctggctgg
     2581 ccaccacccg gcggttggaa aacgatccgc cgtcaaaatc gcggtcgacc ttgtagtcgc
     2641 tgggatgctc ctcgctcccg ccgcgcagga aataggcgtg gagcgaatgg gccggtcggt
     2701 catcgggcac ggtcgcctcg gcggcgttga gcgcctgggc gatcacctcg ccgccaaaca
     2761 cccgtcccac gccgccgcgc ttccgccggc cggcgaagcg gtcttcgcca tcgggcgtga
     2821 cttcaagcag cttgagcagg ccggagacca gccgttcggg tgtcggttcc tcgttcatgt
     2881 ttcgcctttg cgggaccgcg cggacgaggt caatgggggg cttaagcgag cggttaagcg
     2941 cgcggttggg cgatcagtcg ccgaccagcg cgtggggcag gaaccgggcc aggtcgcggg
     3001 tgatcgggcc ggcatcctcg cgcaccgaca tggctacggc atcgtcgccc accacccagc
     3061 tgccgatcac ggcgtggttg ctgccggact gggcgagcgg ggcaaaggcc tggacgatcc
     3121 gcccttccgc gccatagccc tcggccgggc cgcgggtgcg gcgggtgccg tcgaacagct
     3181 cgatatcggc gccctcgcgt gagaagaacg gtttgaccgc gtgcggcccg gccgcgatag
     3241 catcggcgcc gggggtgcct tcgaaggcgg ccggcagcag gttgcggtgg ccgcggtggc
     3301 gttcccacag cagcggcagg atcgccttgt tggagaggat cgccttccac attggttcga
     3361 ggaacagggc ggagctggcg gccagctgcg gggcatagac cgagcgcatc agcgcttccc
     3421 acggatagag cttgaacagc gcgccgatca gggtctgctg gtcatcgacc agctgccccg
     3481 cggcgttcac gccgatcttg tcgatcgcca caaaccgcgc ttccatcccg gcctggctgg
     3541 ccagatcctc cagatagagc acggtctggc gatcctcgac atggtcgtcg accgaagcga
     3601 agtggaccag gctgccgggc gtgaagatcc gggcgaaccg ctcgatc
//
LOCUS       DASUDV010000160         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1242197,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000160 DASUDV010000000
VERSION     DASUDV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242197"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2497
                     /locus_tag="VFV30_03420"
     CDS             <1..>2497
                     /locus_tag="VFV30_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847170.1"
                     /translation="RWTYQGTDYSATSNAVSFDVTQQPVSIATYVATPASTTRLSYSP
                     SICAGGALTLPGGMTGADGLIGASQGATLATGQVLMFSVNAAAHNLNPGAVDSLEAVI
                     TNVSGDRETITIFETGANTGVFVGALPTRASPPAPVPGDCKLSVVPNERIIIAVGQGT
                     ASPIVTAQVNVLADPYGLVFDSETGTPVDGARISLVDALSGAPATVFADDGVTPWPST
                     MISGQPVRDGAGNVYAMLPGEYRFPLAPLGTYRLVIEPPAPYSAPSLATPAQLSPLRR
                     PDGNAFEIVPASFGGSLTLSSPAPVRVDIPLDHPPVAVSVTKTMSRQTALPGDVVFYT
                     VTVRNPDPARIKQGVSLVDSASPLLRMRKDSIRIDGVAPASGAVQFSPDGRVMTLALG
                     DIAPGGLRSVTYAMSVRADAPAGQAANSVTVKDSRYITATTGTILRIDRNDIAARMTL
                     IGRITGGSCTLDETKRFGIAGVRVVLEDGSFAITDADGRYHFEGLVPGSHVVQALGNT
                     LPKGGQFIDCSSSTRSAGSASSKFVIGQGGSLVVADFFASVPEGRRAAAKPVDDKAMG
                     EAALKAERAAAGADIDWLSYGRGATEFLFPAVDHNPRAPVVRVAIRHQVTEKIRLLVN
                     GKPVDPLAFDGAKQAPGGTFAVSVWRGVPLEGEVTHLTAEVLNEDGTKKLAELKRDVH
                     FAAAPARVELVPEKSRLIADGSSRPVLALRLLDRAGRPVHAGLSGEFTLSAPYESGEA
                     LDRMQERALSGLGRSAPRWMVKADDGMAYVELAPTMVSGKLHMEFNFSDRDQRRRQEL
                     DAWIVPGDQPWTLVGLAEGSVGAKSVADNMQRAG"
BASE COUNT          409 a          837 c          827 g          424 t
ORIGIN      
        1 gcgctggacc tatcagggca ccgactactc ggccacctcg aacgcggttt cgttcgatgt
       61 gacccagcag ccggtgtcga tcgcgaccta cgtcgcaacc cccgccagca ccacgcgcct
      121 cagctattcg ccgtcgatct gtgccggcgg ggcgctgacc ctgccgggcg gaatgaccgg
      181 agccgacggg ctgatcggcg ccagccaggg cgccacgctt gccaccggcc aggtgctgat
      241 gttctcggtc aatgccgcgg cccataacct caaccccggt gcggtcgaca gcctggaagc
      301 cgtcatcacc aatgtttcgg gcgatcgcga aacgatcacg atcttcgaaa ccggcgcgaa
      361 taccggagtc ttcgtgggcg cgttgccgac ccgcgccagc ccgccggccc cggtcccggg
      421 cgattgcaag ctttcggtcg ttccgaacga gcggatcatc atcgcagtcg ggcagggcac
      481 cgccagccca atcgtgaccg cacaggtcaa cgtgctggcc gatccctatg gcctggtgtt
      541 cgacagcgaa accggaaccc cggtcgatgg tgcgcggatc agcctggttg acgcgctgag
      601 cggcgcaccg gcaacggtct ttgccgacga cggcgtgacg ccctggccct cgaccatgat
      661 cagcggccag ccggtgcgcg acggcgcggg caatgtctat gccatgctgc cgggtgaata
      721 ccgcttcccg ttggcgccgc tgggcaccta tcgcctggtg atcgaaccgc ccgcgcctta
      781 cagcgcccct tcgctcgcca ccccggccca gctttcgccg ctgcgccgcc ctgacggcaa
      841 tgccttcgag atcgttccgg catcgtttgg cggctcgctg acgctgtcca gtccggcgcc
      901 ggtgcgggtc gacattccgc tcgaccatcc cccggttgcg gtcagcgtca ccaagacgat
      961 gtcgcgccag accgcgctgc ccggcgatgt ggtgttctac accgtaaccg tgcgcaaccc
     1021 cgatccggcg cggatcaagc agggggtcag cctggtcgat agtgcatcgc cgctgctgcg
     1081 catgcgcaag gattcgatcc gcatcgatgg cgtggccccg gccagcggcg cggtgcagtt
     1141 ttcgcccgat ggccgggtca tgaccctggc gctgggcgat atcgcccccg gcggcctgcg
     1201 cagcgtgact tatgcgatga gcgtacgcgc cgatgccccg gccgggcagg cagccaacag
     1261 cgtaacggtt aaggattcac gttatattac agctactacg ggaactattc ttagaattga
     1321 tcgtaatgat atcgccgcgc ggatgacgct gatcgggcgg attaccggtg gcagctgcac
     1381 gctcgacgaa accaagcgct tcgggatcgc cggagtgcgg gtggtgctcg aggatggcag
     1441 cttcgcgatc accgatgccg atggccgcta tcacttcgaa gggctcgttc cgggcagcca
     1501 cgtggtgcag gcgctgggca ataccctgcc caagggcggc cagttcatcg attgctccag
     1561 ttccacccgc agcgccggca gcgccagttc gaagttcgtg atcgggcagg gtggcagcct
     1621 ggtggttgca gatttctttg cctccgttcc cgaaggcagg cgcgccgccg ccaagccggt
     1681 tgacgacaag gccatgggcg aagccgcgct caaggccgaa cgggcggcgg cgggtgccga
     1741 tatcgactgg ctgtcctatg gccgcggcgc gaccgaattc ctgttcccgg ccgtggatca
     1801 caatccgcgc gcgccggtgg tgcgggtggc gatccgccac caggtaaccg aaaagatccg
     1861 cctgctggta aatggcaagc cggtcgatcc gctggccttc gatggcgcca agcaggctcc
     1921 gggcggaacc ttcgcggttt cggtgtggcg cggcgttccg ctggaaggcg aagtcacgca
     1981 cctgaccgcc gaagtgctga acgaggacgg caccaaaaag ctcgccgaac tgaagcgcga
     2041 cgtgcacttt gccgcagcgc cggcacgggt tgagctggtg cctgaaaaga gccggctgat
     2101 cgccgatggt tcgagccgtc cggtgctcgc gctgcgcctg ctcgatcgcg cgggccgtcc
     2161 ggtccacgcg ggtctttccg gcgaattcac gctgagcgcg ccttacgaaa gcggcgaggc
     2221 gctcgaccga atgcaggaac gcgcgctttc gggcctgggc cgcagcgcac cgcgctggat
     2281 ggtcaaggcc gacgatggca tggcctatgt cgaacttgcg ccgaccatgg tcagcggcaa
     2341 gctgcacatg gaattcaact tctccgaccg cgatcagcgc cgccgccagg aactcgatgc
     2401 ctggatcgtg ccgggtgacc agccctggac gcttgttggc cttgccgaag gttcggttgg
     2461 cgcgaagagc gttgccgaca acatgcagcg cgccggc
//
LOCUS       DASUDV010000161         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_468769,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000161 DASUDV010000000
VERSION     DASUDV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468769"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFV30_03425"
     CDS             <1..523
                     /locus_tag="VFV30_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4847171.1"
                     /translation="NQAAAGADVIAPSDMMDGRIGAIRAALEEAGHANVQIMSYAAKY
                     ASAFYGPFRDAVGSRGLLKGDKKTYQMDPANSDEALREVDLDLLEGADSVMVKPGLPY
                     LDIILKVKEAFGVPVFAYQVSGEYAMIEAAVAAGASERDALVLETLTAFKRAGCAGVL
                     TYHAAHAARLLNG"
     gene            516..1067
                     /locus_tag="VFV30_03430"
     CDS             516..1067
                     /locus_tag="VFV30_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847172.1"
                     /translation="MAEFRLETPRLVMRSWRDEDLDPFHATCSDPEVMATLGPVMSRG
                     EVAALIERMHGIEVEHGHCFWALVQQSDQQLIGWCGVIRGSVGPVAGKGEFGWRMARS
                     AWGKGYVTEAARAAIEWFFANRSDDAAWAITNVENRRSRAVMERLGMVYRPDLDFDHP
                     RLAADDPLLRHVTYELPRNAWSM"
     gene            1067..>2128
                     /locus_tag="VFV30_03435"
     CDS             1067..>2128
                     /locus_tag="VFV30_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847173.1"
                     /translation="MTASATSAQRWLFVLTILTGSFLLFLVQPLVARLALPRLGGAPN
                     VWNSAMLVYQALLLAGYFYAHRLSQMPLKRQGRIHLALLAVAGLTLPITLADLPPPAA
                     GMEILWVPALLALTIGPVFFLVSAQAPLMQRWYAAHPGAGAPWALYAASNLGSFGGLI
                     AYPLLAEPLLSASAQSIGWSLGYALLLVLVGLCAWSRSKTADAAVLQEAHSPAPAVGG
                     RRIALWLVLAAVPSGLMLSTTTHLTTDLFAMPLLWVIPLGLYLLSFSVAFADNRVLAR
                     VFTQVAPLFVLIAGGFAASSSNAATLAPVIGSVFLLFIVCVALHSRLYDARPDASQLT
                     LFYLVMSAGGALGGLFTALF"
BASE COUNT          309 a          679 c          703 g          437 t
ORIGIN      
        1 caaccaggct gccgccggcg ccgatgtcat cgctcccagc gacatgatgg acggccggat
       61 cggcgcgatc cgcgctgcac tggaagaggc ggggcacgcc aatgtccaga tcatgtccta
      121 tgcggccaaa tatgcctcgg ccttttatgg accgttccgc gatgcggttg gcagccgcgg
      181 cctgctgaag ggcgacaaaa agacctatca gatggatcca gccaacagcg acgaagcgct
      241 gcgcgaggtt gatctggacc tgctcgaagg cgcggacagt gtaatggtca agccagggct
      301 gccgtacctc gacatcattc tgaaggtgaa ggaagcattc ggcgtcccgg tatttgcata
      361 ccaggtcagc ggcgaatacg cgatgattga ggcagcggtc gcagccggcg ccagtgaacg
      421 tgatgccttg gtgctcgaaa cgctcactgc cttcaagcgg gccggctgtg ccggggtgct
      481 gacctaccac gccgctcacg ccgcgcggct gctgaatggc tgagttccgg ctggaaacgc
      541 cgcggctggt gatgcgttcg tggcgcgatg aagacctcga tccgtttcac gccacctgca
      601 gcgatcccga agtgatggcc acgctcggcc cggtcatgtc gcgcggcgag gtggctgccc
      661 tcatcgaacg gatgcacggg atcgaggtgg agcatgggca ttgcttctgg gccctggtgc
      721 agcaatcgga ccagcagttg atcggctggt gcggggtgat ccggggatcg gtcggtccgg
      781 ttgccggcaa gggcgagttc ggctggcgga tggcccgctc ggcctggggt aagggctatg
      841 tcaccgaagc agcgcgcgcg gcgatcgaat ggttcttcgc caatcggtct gacgatgccg
      901 cctgggcgat caccaatgtc gaaaaccggc gcagccgcgc ggtgatggag cggctgggga
      961 tggtttatcg ccccgatctc gatttcgatc atccgcgcct cgctgctgat gatcccctgt
     1021 tgcgccacgt cacctacgaa ctgccgcgca acgcctggag tatgtgatga ccgcatccgc
     1081 caccagtgcc cagcgctggc tgtttgtcct gacgatcctg acaggcagct tcctgttgtt
     1141 cctggtccag ccgctggtcg cgcggctggc cctgccgcgg ctgggcggcg caccgaatgt
     1201 ctggaacagc gcgatgctgg tctatcaggc gctgcttttg gcgggctact tctatgcgca
     1261 ccgcctttcg cagatgccgc tcaagcggca ggggcggatc cacctggcgc tgctggccgt
     1321 ggcgggattg accttgccga tcaccctggc cgacttgccg ccgccggcgg ccggcatgga
     1381 gatcctgtgg gtcccggcct tgctggcgct gacgatcggg ccggtgttct tcctggtttc
     1441 ggcgcaggcc ccgctgatgc aacgctggta cgctgcccac ccgggcgcgg gcgcgccctg
     1501 ggcgctttac gctgcatcaa acctcggcag tttcggcgga ttgatcgcct atccgttgct
     1561 ggccgaaccc ttgctctcgg ccagtgcgca atcgatcggc tggagcctgg gctatgcctt
     1621 gttgctcgtg ctggttgggc tttgtgcctg gagccgcagc aagaccgccg atgcagcggt
     1681 gctgcaggaa gcccattccc cggcaccggc cgtgggcggg cgccgcatcg cgctgtggct
     1741 ggtgctggcc gccgttccat ccggtctgat gctgtcgacc accacgcacc ttaccaccga
     1801 tctgttcgcc atgccgctgc tctgggtgat cccgctgggg ctctacctgc tcagcttcag
     1861 cgttgccttt gcagataacc gcgtgcttgc ccgcgtcttc acgcaggttg cacccctgtt
     1921 cgtgctgatc gcgggcgggt ttgccgcctc atcgagcaat gcggcaactc tcgcccccgt
     1981 gatcggcagt gtattcctgt tattcatcgt ctgcgttgcg ctccattccc ggctttacga
     2041 tgcccgaccc gatgcctcgc aactgacgct gttctatctt gtgatgtcgg ccggcggcgc
     2101 gctgggcggt ctgttcacgg cgctgttt
//
LOCUS       DASUDV010000162         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_270105,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000162 DASUDV010000000
VERSION     DASUDV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270105"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFV30_03440"
     CDS             complement(<1..810)
                     /locus_tag="VFV30_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX4847174.1"
                     /translation="MFGEPKHVSSGTLKNSFERRSVLIGGLQTAVGVLLAVRMGWIAL
                     VDNEKYKTASESNRVNLTLIPPRRGAILDRNNAPLASNRMEYRIDIIPERLVDAKGTV
                     AKLGTLLGLDAIAMRDLSDKLEKAHGFQPLGVASGIPPEVYDKVLVRLPEMPGVVAAQ
                     GFTRWYPTGPSVGHLLGYVGPANAEEYKAERNPLLVTPGFKIGKDGFEKFFEAQLRGT
                     PGARRAEVTAGGKIVRDLEMREDVPGQSLTLTINGPLQDYAARRLGLESGAV"
     gene            complement(815..1378)
                     /locus_tag="VFV30_03445"
     CDS             complement(815..1378)
                     /locus_tag="VFV30_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX4847175.1"
                     /translation="MPSPLTAPITETLRGFGKQRINRAPSPILAISLPWVLVMLGSLS
                     PTWPVIASAPLLPPFGFLLLVAWQQLRPGLFPVWAGLPLGLFDDLYSGQPFGSAVLLW
                     SVAMLLLDFLELRFPWRGVVLNWLSASGIITAYLVLSLQLANLAGGSASLGLILPQIL
                     LSILAYPIAARLVGAVDRFRLIPIRKL"
     gene            complement(1378..2253)
                     /gene="mreC"
                     /locus_tag="VFV30_03450"
     CDS             complement(1378..2253)
                     /gene="mreC"
                     /locus_tag="VFV30_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX4847176.1"
                     /translation="MVAPSNRRPGHSRRAQYGTFFSYLLALGGLGLGVVLLVTSSSDS
                     SAFSGARASASDLAAPAAQAGAAGRTESIGFFSAIGGFFTRGSRVARLEREVAEARVL
                     LAEQAALKEENARLKALVGLAQTDPRPVAVARLVGSTSSSTRRFAILGAGAQQGIRPG
                     MAVRSPLGLVGRVLEVGEKTSRVLLITDSESTVPVRRANDGIQAYATGRGDGTLQIKL
                     ATTGVNPFRKGDAIVTSGSGGLFRPGEAVAVVVSLTADGAIARPLSDPGMTEFVAVEP
                     IYEEAASAAAAAEPR"
     gene            complement(2305..>2493)
                     /locus_tag="VFV30_03455"
     CDS             complement(2305..>2493)
                     /locus_tag="VFV30_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4847177.1"
                     /translation="ADIVDQGIVLTGGGALIHGLDEYLREETGLPVTIAEDPLSCVAL
                     GTGRAMEDPIFRGVLQTA"
BASE COUNT          398 a          871 c          876 g          348 t
ORIGIN      
        1 gaccgcgccg ctttccagcc cgagccgccg tgcggcatag tcctgcagcg ggccgttgat
       61 agtcagggtc agcgactggc ccggcacgtc ctcgcgcatt tccagatcgc ggacgatctt
      121 gccgcccgcg gtcacttccg cgcggcgcgc gcccggggtg ccgcgcagtt gggcttcgaa
      181 gaacttctcg aacccgtcct tgccgatctt gaagccgggg gtgaccagca gcgggttgcg
      241 ctcggccttg tactcctcgg cgttggccgg gccgacatag ccgagcaggt ggccgaccga
      301 gggcccggtc ggataccagc gggtaaagcc ctgggcggcg accacgccgg gcatttccgg
      361 taggcggacc agcaccttgt catagacttc cggcgggatg cccgatgcca cgccaagggg
      421 ctggaagccg tgggccttct ccagcttgtc ggacaggtcg cgcatcgcga tggcgtccag
      481 accgagcagc gtgcccagct tggccacggt gcccttggca tcgaccagcc gctcggggat
      541 gatgtcgatc cggtattcca tccggttcga ggcgagcggg gcgttgttgc ggtccaggat
      601 cgccccgcgc cgcggcggga tcagcgtcag gttgacgcgg ttgctttccg aggcggtctt
      661 gtacttctcg ttgtcgacca gcgcgatcca gcccatccgc accgccagca gcacgccgac
      721 ggccgtctgc agcccgccga tcaggaccga gcggcgctca aagctgttct tgagggtgcc
      781 cgagctgacg tgcttcggct ctccgaacat cggcctagag ctttcggatc gggatcaggc
      841 ggaaccggtc caccgcgccg accagccgcg cggcgatcgg ataggccagg atcgacagca
      901 ggatctgggg caggatcagg cccagcgagg ccgatccgcc ggccaggttg gccagctgca
      961 gcgacagcac cagataggcc gtgatgatcc ccgatgcgct cagccagttc agcaccacgc
     1021 cgcgccaggg gaagcgcagc tccaggaaat cgagcagcag catggcgacc gaccacagca
     1081 ggaccgccga gccgaacggc tgaccggaat agagatcgtc gaacaggccc agcggaaggc
     1141 ccgcccagac cgggaacagg cccgggcgca gctgctgcca ggcgaccagc agcagaaagc
     1201 cgaagggtgg cagcagcggg gccgaggcaa tcaccggcca ggtcgggctg agcgatccga
     1261 gcatgaccag cacccacggc aggctgatcg ccaggatcgg cgagggcgcg cggttgatgc
     1321 gctgcttgcc gaagccgcgc agggtttcgg tgatcggagc ggtcagcggg ctgggcatca
     1381 gcgcggctcc gctgcggccg cggcgctggc cgcctcttcg tagatcggct caaccgcgac
     1441 gaattcggtc atcccgggat cgctgagcgg gcgggcaatc gccccgtcgg ccgtcagcga
     1501 cacaaccacg gccaccgctt cgcccgggcg gaacaggccg ccagaaccgg acgtcacgat
     1561 cgcatcgccc ttgcggaagg gattcacccc ggtggtcgcc agcttgatct gcagggtgcc
     1621 gtcaccgcgg ccggtggcat aggcctggat cccgtcgttg gcgcggcgca ccggcaccgt
     1681 gctttcgctg tcggtgatca gcagcacccg cgaggtcttc tcgcccactt cgagcacgcg
     1741 cccgaccagc ccgagcggcg agcgcacggc catgccgggc cggatcccct gctgcgcgcc
     1801 ggcacccagg atcgcgaagc ggcgggtgga ggacgaggtc gagccgacaa gccgggccac
     1861 ggccaccgga cgcggatcgg tctgggccag gccgaccagc gccttcaggc gggcgttttc
     1921 ctctttcagc gcggcctgtt cggccagcag cacgcgggcc tcggccactt cgcgttcaag
     1981 ccgggcaacc cggctgccgc gggtgaagaa gccgccgatc gcggaaaaga agccgatgct
     2041 ttcggtccgc ccggccgcgc cggcctgggc cgccggggct gccaggtcgc tcgcgctggc
     2101 gcgggcgccg gaaaaggccg agctgtcgct gctcgacgtg accagcagga ccacgccaag
     2161 gccaagcccg cccagggcga gcagatagct gaagaaagtc ccgtactgcg cccggcggga
     2221 atggcccggg cgccggttgc tgggcgccac catgccggct taaccttcca tgcccgatgc
     2281 gcggcccacg accccagagc ccgctcaggc cgtttgcagc acgccgcgga agatcgggtc
     2341 ttccattgcc cggccggtgc cgagcgccac gcaggacagc ggatcctcgg cgatggtcac
     2401 cggcaggccg gtttcctcgc gcaggtattc gtccagcccg tggatcagcg cgccgccgcc
     2461 ggtcaggacg atgccctggt cgacgatgtc ggc
//
LOCUS       DASUDV010000163         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1705877,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000163 DASUDV010000000
VERSION     DASUDV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705877"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFV30_03460"
     CDS             complement(<1..899)
                     /locus_tag="VFV30_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX4847178.1"
                     /translation="MRTAATALLVVMAGVFLLARHFQDVHPVWGYVLAFSEAAMVGGL
                     ADWFAVTALFRHPLGLPIPHTAIIPENKDRIADTMAQFLRDNFLTPQVVARRLSTMNL
                     AGAAGDWLADPAKGGSSRLRDGAAELLAQVLESLDPERLGTQVRGGLFRQLDRLEVSP
                     LLGQMLTTTIADGRHMPVIDSLVRWAGLTLEDNEELVRDLIHTRANAILRWTGLDERL
                     ANSVLDGLYKLLAEVIVDPEHPLRAKVEEGLAKLAQDLQSDAELRGRVERLKGELIAN
                     PAVSTWWQGVWERLRTAMLDRLRR"
     gene            complement(965..>3220)
                     /locus_tag="VFV30_03465"
     CDS             complement(965..>3220)
                     /locus_tag="VFV30_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4847179.1"
                     /translation="GVLVDDAIVEVENIVRHMRMGKSAYQASIDAADEIGLAVVATTF
                     SIVAVFLPVGLMPGIPGQFFFNFGWTVVVSVLISLAVARMITPMVAAYFLKSAGPAEH
                     GGGIWMERYERLLHFSLDDRKAKAARARIETVRGVPLYHLLPGLAALFVALSPFIMKA
                     APAVGEVAKGPGVGMVLSALILGAPIAYGLVFLLQSLLMAFLNLFIRQREAGFFAWNR
                     FWALRLGARLRDHRVWMLGVAAAALVLSGVLFAILPQKLFPEQNSDFSRVTIEMVPGT
                     TLQQTEAVVAQVANVVKDRPEVERVQERIREGNGSVFITLKKDRDKTSQEFERELTPE
                     LQKIPDARVNFQAVFQAGPPGLGRPISVMLTGSDSAALEAAATQLVDQMKTIPGIVAP
                     RIAADTRRPELIITPRQELAASLGVTTAALSQTIRIATIGDIDQNAAKFSLSDRQIPI
                     RVRLSEESRRDLSVIANLPVQTTSGGSVPLSRVADISFGSGPVSIRRYNQERRVFVGA
                     DLAPGAKEGPIKEQIDKLPIMQNLPPGVGKKPFGADEIFDELITSFQIALASAVLLVF
                     AVLVLLYHRFISPLVNMASLFLAPLGGLIALWIAGQEVTLPVFIGILMLFGIVAKNSI
                     LLIDFAIEEMAQGVPKYEAIVDAGHKRAQPIVMTTVAMTAGMVPTALSLGGDSAWRQP
                     MGTVVMGGLILSTLLTLLIVPAGFSLADGFEKRIGPKLRRAFLTYDPARDRESAPPAS
                     GSIAPGALPAE"
BASE COUNT          545 a         1099 c         1094 g          482 t
ORIGIN      
        1 cgccgcagcc ggtccagcat ggcggtgcgg agccgttccc acacgccctg ccaccaggtc
       61 gagaccgccg ggttggcgat cagttcgccc ttcagccgct ccacccggcc gcgcagctcg
      121 gcgtcgctct gcaggtcctg ggccagcttg gccaggccct cttccacctt ggcccgcaag
      181 ggatgctcgg gatcgacgat cacttcggcg agcagcttgt agagcccgtc gagcaccgaa
      241 ttggcgagcc gctcgtccag tccggtccag cgcaggatgg cgttggcgcg ggtgtggatc
      301 aggtcacgga ccagctcttc gttgtcctcc agcgtaagcc cggcccagcg caccagcgaa
      361 tcgatcaccg gcatgtggcg cccgtcggcg atggtggtgg tcagcatctg cccaagcagc
      421 ggcgagacct ccagccggtc cagctggcgg aacagcccgc cgcggacctg ggtgccgagc
      481 cgttccggat cgagcgattc gagcacctgg gcgagcagct cggccgcgcc gtcgcgcagg
      541 cgcgaggaac cgcccttggc cggatcggcc agccagtccc ccgccgcgcc ggccaggttc
      601 atcgtgctga gccgccgcgc cacgacctgc ggggtcagga agttgtcgcg caggaactgc
      661 gccatcgtat cggcgatgcg gtccttgttt tccgggatga tcgcggtgtg cgggatcggc
      721 aggccgagcg ggtggcggaa cagcgcggtg accgcgaacc agtctgccag gccgccgacc
      781 atcgccgctt cgctgaaggc cagaacatag ccccagaccg ggtgcacatc ctgaaaatgc
      841 cgggccagca ggaacacccc ggccatgacc accagcaagg cggtggccgc ggtgcgcatc
      901 cgccgcgcct tgtctggcgg caggggcgcc gggcgccacc ggcgctgccg aagcggcgtc
      961 ccggtcactc cgccggaagc gcccctgggg cgatcgaacc gctggcgggc ggcgcggatt
     1021 cgcggtcacg ggcgggatcg taggtcagga aggcgcggcg cagcttgggg ccgatccgct
     1081 tttcaaaccc gtcggccagg ctgaagccgg ccgggacgat cagcagggtc agcagggtcg
     1141 acaggatcag gccgcccatc accacggtgc ccatcggctg gcgccaggcc gaatccccgc
     1201 ccagcgacag cgcggtcggc accatcccgg cggtcatcgc cacggtggtc atcacgatcg
     1261 gctgggcgcg tttgtgtccg gcgtcgacga tcgcctcgta cttgggcacg ccctgcgcca
     1321 tttcctcgat cgcgaagtcg atcagcagga tcgagttctt ggcgacgatc ccgaacagca
     1381 tcagaatccc gatgaacacc ggcaaggtca cctcctgccc ggcgatccac agggcgatga
     1441 gcccgcccag tggcgcgagg aacagcgagg ccatgttgac cagcggtgag atgaagcggt
     1501 ggtaaagcag caccagcacc gcgaagacca gcagcacggc cgaggcgagc gcgatctgga
     1561 agctggtgat cagctcgtcg aagatctcgt ccgcgccaaa cggcttcttg ccgacgcccg
     1621 ggggcaggtt ctgcatgatc ggcagcttgt cgatctgctc cttgatcggc ccttccttcg
     1681 cgccgggcgc caggtccgcg cccacgaaga cgcggcgttc ctggttgtag cggcggatcg
     1741 aaaccggccc ggagccaaag ctgatgtcgg caacccgcga cagcggcacc gagccgccgc
     1801 tggtcgtctg gaccggcagg ttggcgatca ccgacaggtc gcggcgcgat tcctccgaca
     1861 ggcggacccg gatcgggatc tgccggtcgg acagcgagaa cttggccgcg ttctgatcga
     1921 tatcgccgat cgtggcgatg cggatcgtct ggctgagcgc cgcggtggtg acgcccaggc
     1981 tggcggccag ttcctggcgc ggggtgatga tcagttcggg ccggcgcgtg tcggccgcaa
     2041 tccgcggggc gacgatgccg gggatggtct tcatctgatc gaccagctgg gtggctgccg
     2101 cttccagcgc ggccgaatcg gagccggtca gcatgaccga gatcggccgg cccagtcccg
     2161 gcgggcccgc ctggaacacc gcctggaagt tgacccgcgc gtcggggatc ttctgcagct
     2221 caggcgtcag ttcgcgttcg aactcctggc tggtcttgtc gcggtccttc ttcagcgtga
     2281 tgaagaccga gccgttgcct tcgcggatcc gttcctgaac ccgctcaacc tccggcctgt
     2341 ccttcacaac gttggcgacc tgcgcgacga ccgcttcggt ctgttgcagg gtggtgccgg
     2401 gcaccatttc aatcgtcacg cggctgaaat cgctgttctg ttccgggaac agcttttgcg
     2461 gaaggatggc aaaaagcacg cccgatagga ccagtgccgc ggccgccacg cccagcatcc
     2521 acacccggtg gtcgcgcagc cgcgcgccca gccgcagcgc ccagaaccgg ttccaggcaa
     2581 agaagccggc ttcgcgctgg cggatgaaca ggttcaagaa ggccatcagc agcgattgca
     2641 gcaggaagac cagaccatag gcgatcggcg cgcccaggat cagcgccgac agcaccatgc
     2701 ccacgccagg gcccttggcc acttcaccca ccgcgggcgc ggccttcatg atgaagggcg
     2761 acagggcgac aaacagcgcg gcaagcccgg gcagcaggtg gtagagcggc acgccgcgca
     2821 cggtttcgat ccgggcgcgg gccgccttgg ccttgcggtc atcaaggctg aagtggagca
     2881 gccgttcgta ccgctccatc cagatcccgc cgccgtgctc ggccggaccg gccgacttca
     2941 ggaagtaggc cgcgaccatc ggcgtgatca tgcgggccac ggccagactg atcagcaccg
     3001 agaccacgac ggtccagccg aagttgaaga agaactggcc cggaatgccg ggcatcaggc
     3061 cgaccggcag gaacaccgca acgatcgaga aggtggtggc aaccaccgcg agaccgatct
     3121 cgtcggcggc atcgatcgag gcctgatagg cgctcttgcc catgcgcatg tgccggacga
     3181 tattctcgac ctcgacgatc gcgtcatcga ccagcacccc
//
LOCUS       DASUDV010000164         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_358510,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000164 DASUDV010000000
VERSION     DASUDV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358510"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFV30_03470"
     CDS             <1..1336
                     /locus_tag="VFV30_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847180.1"
                     /translation="SLTPRGGSGRTILLIDIPGEFLTSLVEGNNDTEIPLALVAALAL
                     SDRLILTLPSDVCVIGPLLQCEHREAGSPDERLERANAILAQKTDKMWDERAALAGRL
                     KALRAIGVETAQDMDRLSAAGLTGDTNKDHIRQLAAAHAAGVESGEHLTRLFADRQAG
                     TASDEDLQKLAAVQAAGFVSGEQWTQLFAACQAGGASKEDVRRLAVAYAAGVENGEQW
                     TALSAAFRDISISLSEKLIREQWVKLDVFRERVSNALGRAAHVVHKHGRNTFLDKPAN
                     TWLEGYSQAAAERLPPIHGGSLAVLTKADRVLPLLTDRDFGVKPGRDWPHLPERQANR
                     DALQASGWRSALARHIGDPRLVLAMTLPKLVSQLDGWLPNMRYEWASAEWQASGWSVN
                     MDLSHDHRGVRELAHWIARGGPDRTIAPAVLARIETMRAYLEGRESAGGKSR"
     gene            1333..2052
                     /locus_tag="VFV30_03475"
     CDS             1333..2052
                     /locus_tag="VFV30_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847181.1"
                     /translation="MSQKIDLPQRPKLTALIDYLFTATPLRAVMHAARMVGIGAALLL
                     LAMLFGRFTGQWIELDEPETAMAFAEWPAVPDAVKPMIMSDDGSAQTLSLQDVRNRLI
                     SVNANARRAAEGQELIDVTDKPVAELAASTDPALAAYADWVDGSACSLSGWKGESRKA
                     AAALVRNGVWGCGGVKRAAAGMPLVIELGFWLGGFLSLVLAFLILGYIGYVIRGTWHA
                     IQEHRAHDALYGSWAGKRGPL"
BASE COUNT          345 a          693 c          768 g          330 t
ORIGIN      
        1 ttcgctgaca ccgcgcggcg gcagcgggcg gacgatcctg ctgatcgaca ttccgggcga
       61 attcctgacc tcgctggtcg aaggcaacaa cgacaccgaa attccgctgg cgctggtcgc
      121 ggcgctggcg ctgtcggacc gactgatcct gaccctgccc agcgatgtct gcgtgatcgg
      181 gccgctgctc caatgcgagc accgcgaagc cgggtcgccc gatgagcggc tggaacgggc
      241 caacgcgatc ctggcccaga aaaccgacaa aatgtgggac gagcgagccg ccctggccgg
      301 ccggctcaag gcgctgcgcg caatcggtgt cgaaacggcg caggatatgg atcgcctgtc
      361 tgcggctggt ctgacgggcg atacgaacaa ggatcacatt cgccagcttg cggcagccca
      421 tgccgccggg gttgagagcg gcgaacattt gacccggctg ttcgccgacc gccaggctgg
      481 caccgcaagc gatgaggacc ttcagaagct tgccgcggtg caggcggcag gttttgtgag
      541 cggcgagcaa tggacccagc tgtttgccgc ttgccaggcg ggcggtgcca gcaaagagga
      601 tgttcggcgg cttgcggttg cctatgccgc cggcgttgag aatggcgagc aatggaccgc
      661 gctgtccgcc gcattccgcg acatctcgat cagcctcagc gaaaagctga tccgcgagca
      721 gtgggtcaag ctcgacgtgt tccgcgagcg ggtcagcaac gcgctgggcc gggcggcgca
      781 tgtggtccac aagcacggcc gcaacacctt cctcgacaag cccgccaaca cctggctgga
      841 gggctattcc caggccgcgg ccgagcggct gccgccgatc cacgggggat cgctggcggt
      901 gctgaccaag gcggaccggg tgctgccgct gctgacggac cgcgatttcg gcgtgaagcc
      961 cggccgcgac tggccgcacc tgcccgagcg gcaggccaac cgcgatgccc tgcaggcctc
     1021 gggctggcgc agcgcgctgg cgcggcacat cggcgatccc cggctggtcc tggcgatgac
     1081 cctgcccaag ctggtctcgc aattggacgg gtggctgccc aacatgcggt acgaatgggc
     1141 cagcgcggaa tggcaggcca gcggctggtc cgtgaacatg gacctcagtc acgatcaccg
     1201 gggcgtgcgc gagctggcac actggattgc gcgcggcggg cccgaccgga caatcgcgcc
     1261 tgccgttctg gcccggatcg agacgatgcg ggcctatctt gaaggccggg aaagcgcggg
     1321 gggcaaaagc cgatgagcca gaagatcgac ctgccgcagc ggcccaagct gaccgcgctg
     1381 atcgactatc tgttcaccgc cacgccgctc agggcggtga tgcacgccgc gcggatggtg
     1441 gggatcgggg cggcgctgct gctgctggcc atgctgttcg ggcggttcac cgggcagtgg
     1501 atcgagctgg acgagcccga gaccgcgatg gcctttgccg agtggccggc cgtgcccgac
     1561 gcggtcaagc cgatgatcat gtcggacgat ggcagcgccc agacgctgag cctgcaggac
     1621 gtgcgcaacc ggctgatctc ggtcaacgcc aacgcccgcc gcgcggccga agggcaggaa
     1681 ctgatcgacg tcaccgacaa gccggtggcc gaactcgccg cgtccaccga tccggcgctc
     1741 gccgcctatg ccgactgggt ggacggatcg gcctgttcgc tctccggctg gaagggcgaa
     1801 tcccgcaagg ctgccgccgc gctggtccgc aatggggtct ggggctgcgg cggggtgaag
     1861 cgggccgcgg ccggcatgcc cctcgtgatc gaactgggtt tctggctggg cgggttcctt
     1921 tcgctggtcc tggctttcct gatcctgggc tatatcggct atgtgatccg cgggacctgg
     1981 cacgccattc aggaacaccg cgcccatgac gcgctctatg gcagctgggc cggcaagcgg
     2041 ggaccgctct gacagtggag gtgggggcgc tgacctggac ccagggcatc agcctggcgc
     2101 tgttcggcgg gatcagtgtg atcctggccg tgctcg
//
LOCUS       DASUDV010000165         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_932992,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000165 DASUDV010000000
VERSION     DASUDV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932992"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1222
                     /locus_tag="VFV30_03480"
     CDS             182..1222
                     /locus_tag="VFV30_03480"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4847182.1"
                     /translation="MTMPQSQTIEPDEAWRAVLARDRAFDGRFVTGVLTTGIYCRPSC
                     AARHPARANVRFFATGAAAREAGLRPCKRCLPDDVGRDERAVLAAIGAIKAAGQPLAL
                     AALAAETGYSPSHFQRVFTRHTGLSPAAYARALQAERAAEALSEEGSVTEAIYAAGYS
                     GPSRFYEANEGRLGMTASAWANGGKGVTIHWAVVPTSLGAMLVAATDKGVCRLSFNEG
                     REALERRFPNAKLIEGGDEFAELLSEVIAAVEMPGDFSHIPIDVKGTAFQEAVWRELR
                     RIPLGETRTYAQLAAAVGRPKAVRAAGSANGANHVSVLIPCHRVVNTGGGLGGYAWGL
                     DIKRKLLEKEAK"
     gene            1219..1995
                     /locus_tag="VFV30_03485"
     CDS             1219..1995
                     /locus_tag="VFV30_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX4847183.1"
                     /translation="MNAKVEAFRALHVPGDPLVLFNIWDAGSARAVAGAGARALATGS
                     YGVAEAQGFKDGETFPFERALDVLAGVLRVTDLPVTFDMESGYGDAPDGVAASVARAV
                     EAGAVGINMEDRMPGLAELNPIDVQARRIAAAASTGLFVNARCDVFRGKPAESHGTEL
                     VAQVLERARAYADAGASGLFVPFTTSHACIGAICEASPLPVNILWTPASAGGFSGKAE
                     LAQLGVARISHGHRPWAAAMDWLAQTAGRVLAGEAPPYDG"
     gene            2038..>2349
                     /locus_tag="VFV30_03490"
     CDS             2038..>2349
                     /locus_tag="VFV30_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847184.1"
                     /translation="MNDMIHSDRPGLLRRIAAFPLTLLVIEAVVVVGVAGGYSVLARR
                     LGVMDDGPLHFVGALGLALAVIGAFYGLTRWVERRDDPGFSLAGAPRELGLGLLAGTA
                     LF"
BASE COUNT          365 a          724 c          854 g          406 t
ORIGIN      
        1 cggctgccag ccgcaggggc aaggccagca cgagggcggc ggcaagcagg cgcaacagac
       61 gggtcatcgg catctccagt cactccaccc tgccgcgtgc gggttttgac cgcaaggggc
      121 gggatgcagc ctgcacgcaa agctgacagg gcttgcacca cgaaggagac ctgccattac
      181 tatgaccatg ccccagagcc aaacgatcga gcctgacgag gcgtggcgcg ccgtcctcgc
      241 gcgcgatcgg gcgtttgacg gacgctttgt aaccggggtg ctgaccaccg ggatctattg
      301 ccggccaagc tgcgcggcgc gccatccggc ccgcgccaac gtgcgcttct ttgcgacagg
      361 tgcggcggcg cgggaagcgg ggctcaggcc ctgcaagcgc tgtcttcctg atgatgtggg
      421 ccgcgatgaa cgggctgttc tggcggcgat cggggcgatc aaggccgccg gtcagccgct
      481 ggcgctggcc gcgctggccg cggaaaccgg ctattcgccc agccacttcc agcgggtgtt
      541 cacccgccac accgggcttt cgccagccgc ctatgcccgt gcgcttcagg ccgaacgcgc
      601 ggccgaggcg ctgagcgagg agggcagtgt gaccgaggcc atctatgccg cgggctattc
      661 gggtccgtcg cggttctacg aagcaaacga agggagactg ggaatgacgg cttcggcctg
      721 ggcgaacggc ggcaagggcg tgacgatcca ttgggcggtg gtgcccacca gccttggcgc
      781 gatgctggtt gcggcgactg acaagggcgt gtgccggctt tcgttcaacg aagggcgcga
      841 ggcgctcgag cggcgctttc ccaatgccaa gctgatcgaa ggcggcgatg aattcgccga
      901 actcctgtcc gaggtgattg cagctgtcga aatgcccggc gatttcagcc acattccgat
      961 cgacgtgaag ggcaccgcct ttcaggaagc ggtctggcgc gaattgcggc gcattccgct
     1021 tggcgaaacc cgcacctatg cgcagcttgc cgcagccgtg ggccggccca aggcggtgcg
     1081 cgcggcgggt agcgccaatg gcgccaacca cgtttcggtg ctgatcccgt gccaccgggt
     1141 ggtcaacacc gggggcgggc tgggcggtta cgcctggggg ctcgatatca agcgcaagct
     1201 actggaaaag gaagcgaaat gaacgccaag gttgaagcgt tccgggcctt gcacgtgccc
     1261 ggcgatccgc tggttctgtt caacatctgg gatgccggca gcgccagggc ggtggctggt
     1321 gccggggcca gggcgcttgc caccggcagc tatggcgtgg ccgaggcgca gggcttcaag
     1381 gatggcgaaa cctttccgtt cgaacgtgcg ctcgacgtgc tggcgggcgt gctgagggtc
     1441 accgatcttc cggtcacttt tgatatggaa tcgggctacg gcgatgcgcc ggacggggtt
     1501 gcggcatcgg tcgcccgtgc ggttgaagca ggggcagtgg gcatcaacat ggaagaccgg
     1561 atgcccggct tagccgaact caatccgatc gacgtgcagg cccggcggat cgccgcggcg
     1621 gccagcaccg gcctgtttgt caacgcgcgc tgcgatgtgt ttcgcggcaa gccagccgaa
     1681 agtcatggca ctgaactggt cgcccaggtg ctcgaacgcg cgcgggccta tgccgatgcc
     1741 ggggcgagcg ggctgttcgt gccctttacc accagccatg cctgcatcgg ggcgatctgc
     1801 gaggcatcgc cgctgccggt caatatcctt tggaccccgg catctgccgg cggattttcg
     1861 ggcaaggccg aactggctca gctgggcgtg gcgcggatca gccacggcca ccgtccctgg
     1921 gccgcggcga tggactggct ggcccagacc gcaggccggg tgctggcggg cgaggcgccg
     1981 ccctacgacg gctagggcaa gacttgccaa acgtgatcga gcggctaacg gttgtgtatg
     2041 aacgacatga tacactctga tcggccgggc ctgctgcgcc ggatcgccgc ctttccgctg
     2101 acccttctgg tgatcgaggc ggttgtcgtg gtgggcgtgg cggggggcta ttcggtcctc
     2161 gccaggcggc tgggagtgat ggacgatggc ccgctgcatt ttgtcggggc cctgggtctg
     2221 gcgctcgcgg tgatcggtgc tttttatgga ctgacccggt gggttgagcg ccgggacgat
     2281 ccgggctttt cgttggccgg tgcgccgcgc gaacttggcc tcgggctctt ggccggaacc
     2341 gcgctgttc
//
LOCUS       DASUDV010000166         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1419042,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000166 DASUDV010000000
VERSION     DASUDV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419042"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFV30_03495"
     CDS             <1..338
                     /locus_tag="VFV30_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847185.1"
                     /translation="SPQGARRAWGIYAAKNPELKGYRMTITQAKVRGKTYFRVAAAGL
                     NGKGASGLCSTVKARGMACFAYALPAKGGNAPAYARAAKPAVKGAVKAAPPAVAAKAP
                     SGPSNARRR"
     gene            complement(409..1644)
                     /locus_tag="VFV30_03500"
     CDS             complement(409..1644)
                     /locus_tag="VFV30_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4847186.1"
                     /translation="MSTPRPLTIYNGQLLLPGARPVPGSLRCADGRIVAVGGAEPAGD
                     DLVIDARGALIAPGLVDFGVFAIDKPAFHFGGITRAALMPDQGVVLDKPSAVRFAAQS
                     GKPDLWVHPLAAATRELEGMELAELALMRDAGARAVATGRRWIGDSGVMHRLMSYCAM
                     LGLTVVAHSEDAGLAGNAVATAGEMATRLGLPSAPAAAEALAIGRDLALVELTGCHLH
                     LRQVTTARGLDLVRAAKARGLPVTAGVTPAHFMLSDVAVGDFRTFTRLSPPLRCDSDR
                     KAVIAAIADGTIDVIASGHDPRGPEDKRLPFADAEPGMAGAETLLPLTLSLVRDRVID
                     LARAFELVATNPARLLGVDAGRLVQGAEADIALIDPVRPWVVDSDQMAAAAGNTPFDK
                     QGVEGRVTGLFKGGVAIEI"
     gene            complement(1641..2633)
                     /locus_tag="VFV30_03505"
     CDS             complement(1641..2633)
                     /locus_tag="VFV30_03505"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX4847187.1"
                     /translation="MTSPETSAAGRYPVGSAAFAHRDLTGLAGLAPWEILFLLDEAEQ
                     WVELNRQPHKRVDALAGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMQVAQSSVKK
                     GETLIDTAMTLNAMHADAMVIRHQSSGAVRLIAEKVDCPVLNAGDGQHEHPTQGLLDA
                     LTIRHAKGDFAGLAVTICGDILHSRVARSNIHCLTTLGAEVRVCAPPALMPADIEALK
                     VKPFHDFDAALAGADVVMMLRLQQERMSGQFIPSPREYRHLYGLTLDRLARARSDALV
                     MHPGPMNRGIEIDSNVADLAGRSAITRQVEMGVAIRMACLDVLTRRTRKVEGWS"
BASE COUNT          461 a          886 c          864 g          444 t
ORIGIN      
        1 tctcgccgca gggcgcgcgc cgcgcctggg gcatctatgc tgcgaagaac ccggaattga
       61 agggttaccg catgaccatc acccaggcca aggtgcgcgg gaagacctat ttccgggtcg
      121 ctgcggctgg tctgaatggc aagggcgcct ctggcctgtg ctcgaccgtc aaggcgcgcg
      181 gcatggcctg cttcgcctat gccctgcctg ccaagggcgg caatgcaccg gcctatgccc
      241 gggccgccaa gcccgcggtc aagggcgcag tgaaggctgc accgccggcg gttgccgcca
      301 aggctccttc cggcccgagc aacgctcgcc ggcgctaagt tctggaaccg caggagcggt
      361 tcgcaaatcc aggcaggccc cctctcgcgg ctgcgagcgg gggtcttgtc atatctcgat
      421 cgcgaccccg cccttgaaca gcccggttac ccggccttcg accccttgct tgtcgaacgg
      481 ggtgttcccg gctgctgctg ccatctggtc cgaatcgacc acccacggcc ggaccggatc
      541 gatcagggcg atatcggcct cggcaccctg aaccagcctg ccggcatcga cgcccagcag
      601 gcgggccgga ttggttgcaa ccagttcgaa agcgcgagcc aggtcgatca cccgatcgcg
      661 caccaggctg agtgtaaggg gcagcagcgt ttcggccccg gccatgcccg gttcggcatc
      721 ggcaaagggc aggcgcttgt cctcgggtcc gcgcgggtcg tgccccgatg cgatcacgtc
      781 gatcgtgcca tcggcaattg cggcgatcac cgccttgcga tcgctatcgc agcgcagtgg
      841 cggcgaaagc cgggtgaagg tacgaaaatc gcccaccgca acatcggaca gcatgaagtg
      901 ggcaggggta acgcctgcgg tcaccggcaa gcccctggcc ttggccgccc ggaccagatc
      961 gaggccgcgc gcagtcgtga cctggcgcag atggaggtgg catccggtca gttcgaccag
     1021 ggcgagatcg cgcccgattg ccagcgcctc tgccgcggct ggagccgagg gaaggcccag
     1081 ccgagtggcc atttcacccg cagttgccac cgcattaccg gcaagtccgg catcttcgct
     1141 atgagccaca actgtcagcc ccagcatcgc gcaataggac atcagccggt gcatcacccc
     1201 gctatcgccg atccagcgcc gcccggtggc aacggccctg gctccggcat cgcgcatcaa
     1261 ggcgagctcg gccagctcca tgccctcaag ttcgcgggtc gcggcagcga ggggatggac
     1321 ccacagatcg ggcttgcccg attgcgccgc aaaacgaacc gcggatggct tgtcgagcac
     1381 gacaccctgg tcgggcatca gggccgcacg ggttatgccg ccgaagtgga aggccggctt
     1441 gtcgatcgcg aagacgccga aatcgacaag gcctggggca ataagcgcgc cgcgcgcatc
     1501 gatgaccagg tcatcgccgg cgggctcggc accaccaaca gcgacaatcc ggccatctgc
     1561 gcagcgcagt gatcccggca ccgggcgtgc cccgggaagc agcagctggc cattgtagat
     1621 ggtgaggggg cggggcgtgc tcatgaccag ccctccacct tgcgggttcg ccgcgtcagc
     1681 acatcaaggc aggccatccg gatcgccacg cccatttcga cctggcgggt aatggccgat
     1741 cgcccggcca gatcggcgac attgctgtcg atctcgatcc cgcggttcat cgggcctggg
     1801 tgcatcacca gtgcatcgga acgggccctg gccagccggt caagcgtcag cccgtaaagg
     1861 tggcgatatt cgcgcgggga tgggatgaat tggccgctca tccgttcttg ctgcaggcgc
     1921 agcatcatta ccacgtcggc ccctgccagc gcggcgtcga aatcgtgaaa cggcttgacc
     1981 ttcagcgcct cgatatcggc gggcatcagg gccggggggg cacaaacccg cacttccgcg
     2041 ccgagcgtgg tcaggcaatg gatgttcgat cgggccaccc ggctgtgcag gatgtcgccg
     2101 cagatcgtca ctgccaggcc cgcgaaatcg cccttggcat ggcggatggt cagtgcgtcg
     2161 agcagtcctt gtgttggatg ctcgtgctgg ccatcgcccg cattgagcac cgggcaatcg
     2221 accttttcgg cgatcagcct taccgcgccg gagctttgat ggcggattac catggcatcg
     2281 gcgtgcatcg cgttcagcgt catcgccgta tcgatcagcg tttcgccctt cttcacgctt
     2341 gactgggcaa cctgcatgtt gaccacatcg gcgccgagcc gctttccggc gatttcgaac
     2401 gaaagcaacg ttcgggtgct gttttcgaag aacgcgttga tgatcgtaag accggccagc
     2461 gcatcgaccc gcttgtgcgg ctggcggttc agttcgaccc attgttccgc ctcatccagc
     2521 aggaacagga tctcccacgg agccagcccg gcaaggccgg tcaggtcgcg atgcgcaaat
     2581 gccgcgctgc cgactggata gcggcccgct gcagaggttt ctggcgatgt cattgaagac
     2641 agcgccttag accgg
//
LOCUS       DASUDV010000167         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2103668,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000167 DASUDV010000000
VERSION     DASUDV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103668"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VFV30_03510"
     rRNA            complement(<1..130)
                     /locus_tag="VFV30_03510"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            655..1692
                     /locus_tag="VFV30_03515"
     CDS             655..1692
                     /locus_tag="VFV30_03515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX4847188.1"
                     /translation="MSEVASPSPAHANPRVAVIVPAYGVAEFLGAALDSVLAQSFQDW
                     ECVVIDDGAPDDVAGAVAPYLADPRIRFLATANQGVAAARNTAIAASTAPLVALLDGD
                     DLIRPDYLAEIVRALDSDPRVRLVTVNAEMFGAVTPGKLCFEEKQGKGDGVRGTLADV
                     LDRSFGVYIGSAFRRADFDRIGGFDAGLTLCEDLDLWVQLMLLGGDAYYVDRVLADYR
                     VRAGSASSKTQKMLTGVIEVYQKAQAALPADAPEQPLLARLIAENEEALEFERAVDQI
                     VAGNAREGVARLRKTRRQVQGPVWTASFALWRLFPGLARPMLSWRRQAHARGGAPQGL
                     SALSALVGFGV"
     gene            1697..3169
                     /locus_tag="VFV30_03520"
     CDS             1697..3169
                     /locus_tag="VFV30_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX4847189.1"
                     /translation="MSAMSVRSAAIWAIASQYTAFAVQFATSVVLARWFIDPHQLGLF
                     SIAFAAITLVAFLQDFGIGRYIMGERDLTEEKVQTAFTLSITVAWSIAAVAILLAWPV
                     AWFYQNPELLTLSLVIGCSYLMVPLAIVPQALCQRRMDFKSNAMIEVGAALSNAAVSL
                     TLAMQGMGALALAWGAFAQQAARMVVSQWRSGGMLPWPWRMQGIRPILALGGTNTVQV
                     FCSTITIRVPELLIGRVLNEAAVGLFARATGLAAQLRLLVSGAVTGVFYPAFRKVRDE
                     GQPLGPPYLRVVAAYTGITWPAMAGIAVLAEPLVAMLYGPRWIAAAPLLAWIALAQLC
                     YGSVPLNLDLPVLLGRLRDMVRLNLVETISSLVLLAVAAPFGLKWIAISRLAHGLFFI
                     ALYMPFMRTMLDFRWRDLLALYGKSLIATLAAIAPVLAGYGLVAGPAEAGLGQLALGV
                     IGGVLAWLLALAVLRHPLLGEIEAILSDLLAKRPAARPNR"
     gene            3229..4137
                     /locus_tag="VFV30_03525"
     CDS             3229..4137
                     /locus_tag="VFV30_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX4847190.1"
                     /translation="MAVNPLLIPAVSLMLSLPVLAGQEGSGQAGEVDIIELGTDQRDR
                     MTVPVRIGAHGPFEFMIDTGSQNTVISTSLATRLALPTAKRVRLIGVAGTKSVDSVEI
                     EQIDLGTRSYYGILAPLLERRDIGADGILGLDSLQGQRVQIDFKKGTIAVADAKTLGG
                     NRGYDIVVNARRRSGQLIMAEAKIDGIRVDVVIDTGGESSIGNRALQKALARRHRGEQ
                     AELISVTGQTITADINYASRLTLGEMSFTNVQIAYADSPSFEVLELTKRPALFLGMRD
                     LRQLDRLAIDFAARKVFFDLPSSAFH"
BASE COUNT          651 a         1467 c         1437 g          808 t
ORIGIN      
        1 tccgaagcaa agggcagatc cccacgcgtt acgcacccgt gcgccactaa ccccgaaggg
       61 ttcgttcgac ttgcatgtgt taggcatgcc gccagcgttc gttctgagcc aggatcaaac
      121 tctcaagttt gtgtcacgat aaccacggca cgggtaaacc cgctgaccgc agtatcgaac
      181 ttcaggagcc gatacctgca cttgtcaaac gtaatggata cgaaggacat atgaggtaac
      241 gacttaatca acccatacac ggagcctgaa gctccccgga tggggcgccg tcgcccacat
      301 gtcccttcat ctaaaccgac aatgtcaaag agccaccgac aaaatcaggc ggacaaccat
      361 cgtaccccgc ttttgagttc gggggacttg ttgtccgtct atgttggcga ccggggcggt
      421 ggaccgtttg aagcggcgtt cccgtccggt gaacgggcct ctaagcctga cctttgattc
      481 ggtcaacgcc tttttgcaat tttatttcaa cgtggccgaa aaccgcagga ttccgtgggt
      541 tttgagtttc attcggatgc cggaggggcc gaatcgttcc caaattcggt aactttccga
      601 ttcggatccc ctgcgtaagg ccaactttaa cgccccccgc ctatatccgc ggccatgtct
      661 gaggtcgctt ccccttcccc cgcgcacgcg aatccccgcg tggccgtgat cgttccggcc
      721 tatggggtgg ccgagttcct tggcgcggcg ctcgattcgg ttctggccca gtcgttccag
      781 gactgggaat gcgtcgtgat cgacgatggc gcgcccgatg acgtggccgg cgccgttgct
      841 ccttatctgg ccgatccgcg catccggttc ctggccaccg ccaaccaggg cgttgccgcc
      901 gcccgcaaca ccgcaatcgc cgccagcacc gccccgctgg tggccctgct ggacggcgac
      961 gacctgatcc ggccggacta cctggccgag atcgtccgcg cgctggacag cgatccgcgg
     1021 gtccgcctgg tcaccgtcaa cgccgagatg ttcggcgcgg tcacgcccgg caagctgtgc
     1081 tttgaagaaa agcagggcaa gggcgatggt gtgcgcggca ctctggccga cgtgctcgac
     1141 cgcagctttg gcgtctatat cggttcggcc ttccgccgcg ccgactttga ccggatcggc
     1201 ggcttcgatg ccgggctgac cctgtgcgag gatctggacc tgtgggttca gctgatgctg
     1261 ctgggcggcg atgcctatta tgtcgacagg gtgctggccg attaccgggt ccgggccggg
     1321 tcggcctcgt ccaagaccca gaagatgctg accggcgtga tcgaggtcta tcagaaggcc
     1381 caggccgccc tgccggccga cgcgcccgag cagccgctgc tggcccggct gattgccgag
     1441 aacgaggaag cgttggagtt cgagcgcgcg gtcgatcaga tcgtcgctgg caacgcccgc
     1501 gaaggggtgg cacgcctgcg caagacgcgg cgccaggtgc agggtccggt ttggaccgca
     1561 tccttcgcgc tgtggcgcct gttccccggg ctggcccggc cgatgctgag ctggcgccgc
     1621 caggcccacg cccgcggggg cgccccgcag ggcctatcgg cgctctccgc gctggtgggc
     1681 ttcggggttt gatccgatga gcgccatgtc ggtccgttcg gccgcgatct gggcgatcgc
     1741 gtcgcaatac accgcctttg cggtgcagtt cgcgaccagc gtcgttctgg cgcggtggtt
     1801 catcgacccg caccagctgg gcctgttctc gatcgccttt gctgccatca cgctggtggc
     1861 cttcctgcag gacttcggga tcggccgtta catcatgggc gagcgcgacc tgaccgagga
     1921 aaaggtccag accgctttca ccctttcgat caccgtggcc tggagcattg ccgccgtcgc
     1981 gatcctgctg gcctggccgg tggcctggtt ctaccagaac ccggaactgc tgacgctcag
     2041 cctggtgatc ggctgttcct atctgatggt cccgctggca atcgtcccgc aggcgctgtg
     2101 ccagcggcgg atggacttca agtccaacgc gatgatcgag gttggcgcgg cgctgagcaa
     2161 cgcggctgtc tcgctgaccc tggccatgca gggcatgggc gcgctggcgc tggcctgggg
     2221 cgcctttgcc cagcaggcgg cgcggatggt ggtgagccag tggcggtccg gcggcatgct
     2281 gccctggccg tggcggatgc aggggatccg cccgatcctg gcgctgggcg gcaccaacac
     2341 ggtgcaggtg ttctgcagca cgatcacgat ccgggtgccg gaactgctga tcggccgcgt
     2401 cctcaacgaa gccgcggtcg gcctgttcgc ccgcgccacg ggcctcgccg cgcagctgcg
     2461 gctgctggtg tcgggcgcgg tgaccggagt cttctatccc gccttccgca aggtccgcga
     2521 cgagggccag ccgctcggcc cgccctatct gcgggtcgtg gcggcctata ccgggatcac
     2581 ctggccggcc atggccggca tcgccgttct ggccgaaccg ctggtggcga tgctttatgg
     2641 cccgcgctgg atcgcggccg cgccgctgct ggcgtggatc gccctggcgc agctgtgcta
     2701 cggctcggtc ccgctcaacc ttgacctgcc ggtcctgctg gggcggctgc gcgacatggt
     2761 gcggctcaac ctggtcgaga cgatctcctc gctggtgctg ctggcggtgg cggcgccgtt
     2821 cgggctcaag tggatcgcga tctcgcggct ggcccacggg ctgttcttca tcgcgctcta
     2881 tatgccgttc atgcgcacca tgctcgattt ccgctggcgc gacctgctgg cgctctatgg
     2941 caagagcctg atcgccaccc tggccgcgat cgcgccggtg ctggccggct atggcctggt
     3001 ggccggaccg gccgaggcgg gcctgggcca gctggcgctg ggcgtgatcg gcggggtgct
     3061 ggcctggctg ctcgccctgg cggtgctgcg ccatcccttg ctgggcgaga tcgaggccat
     3121 cctgagcgat ctcttggcca aacgaccagc cgcccggccc aatcgatagg cggctttgct
     3181 ctccggcacc gattggcgta gagtcctccg cggaagagga gccgaaccat ggccgtcaat
     3241 ccgctgctga tacccgccgt ttcgctgatg ctgtcgctgc cggtgctggc cggacaagag
     3301 ggcagcggcc aggccggaga ggtcgatatc atcgagctgg gcaccgacca gcgcgacagg
     3361 atgaccgtgc cggttcggat cggcgcgcac ggaccgttcg aattcatgat cgacaccggc
     3421 tcccagaaca ccgtcatttc cacttcgctg gcgacccggc tggcgctgcc cactgccaag
     3481 cgggtgcggc tgatcggggt ggccgggacc aagtcggtcg attcggtgga aatcgaacag
     3541 atcgatctcg gcacgcgttc gtattacggc atcctcgcgc ctttgctgga gcggcgcgat
     3601 atcggcgccg acgggatcct cgggctcgac agcctgcagg gccagcgggt ccagatcgat
     3661 ttcaagaagg gcaccatcgc ggtggccgat gccaagacgc tgggcggcaa ccgcggctat
     3721 gacattgtcg tcaacgcgcg gcgccgctcg ggccagctga tcatggccga ggccaagatc
     3781 gacggcatcc gggtcgacgt ggtgatcgac accgggggcg aatcctcgat cggcaaccgc
     3841 gcgctgcaaa aggccctcgc ccgccgccac cggggcgagc aggccgagct gatctcggtc
     3901 accggccaga ccatcaccgc cgacatcaac tatgccagcc ggctgaccct cggcgagatg
     3961 agcttcacca acgtgcagat cgcctacgcc gattcgccct cgttcgaggt tctggagctg
     4021 accaagcgcc cggcgctgtt cctggggatg cgcgacctgc gccagctgga ccggctggcg
     4081 atcgactttg cggcgcgcaa ggtgttcttc gatctgccgt ccagcgcgtt tcactaggcc
     4141 ccggaccaat ccgccgcacg ctgcgccctt cccttttgtc cggccccgcc ccacatgagg
     4201 ccgatgcacg caccggccac gcccgcacct gactccgcaa cccgccatga tagctggggc
     4261 acgctgcgcc gcttcctgcc ctatctctgg ccggccgatc gcccctcgct gaagcgcaag
     4321 atcgtgctgg cggtgctgct ggtcctggcc agcaaggccg tga
//
LOCUS       DASUDV010000168         6700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1352848,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000168 DASUDV010000000
VERSION     DASUDV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352848"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1203
                     /locus_tag="VFV30_03530"
     CDS             172..1203
                     /locus_tag="VFV30_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX4847191.1"
                     /translation="MNAITPITTTLKDWESDQEVRWCPGCGDYAILKAVQRTLPEIGA
                     DPAKTVFVSGIGCSSRFPYYVESYGFHTIHGRAPAVATGIKLANPELDIWLVTGDGDG
                     MSIGGNHTMHLLRRNLNCQVLLFNNEIYGLTKGQYSPTSREGTHSPTTPLGSVDRPAL
                     PCAFALGAGARFVARGFDVSKHLPDVLKAAHAHKGAAFVEIFQNCIVYNKDVFNDFAA
                     PKGAEDRQLWLKNGEPMLFAGGAQGIALDAEKLTLKVVDVVDGDWQAAGVIVHDVTNR
                     SIAHMLVEMPFGPFPMALGVIYDDPRPTYEDAVLGQDTKAREGKSTDLARLLAKGQTW
                     TVSEDGPEL"
     gene            1287..1679
                     /locus_tag="VFV30_03535"
     CDS             1287..1679
                     /locus_tag="VFV30_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX4847192.1"
                     /translation="MSRREEIEALARTVVDCGFQLHRDIGPGLLESVYQTLMLASIQE
                     RGLAVAKEVAVPIKFKGVVIDNAFKADLIVEDRLLIELKSTERVAPVHAKQVLTYLRL
                     LNMPLGLLMNFGQPTFKDGLQRVVNNHH"
     gene            1734..2657
                     /locus_tag="VFV30_03540"
     CDS             1734..2657
                     /locus_tag="VFV30_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4847193.1"
                     /translation="MDNITHSLIGAALGQAGLKRKTGLAMPALIIGANLPDIDAGCVV
                     YGIESLAMRRGITHGPIALAVLPLLLAGLLWGYDRWQAGRGKRPVGRLPVRFGWLLAL
                     CYLGTLSHPLFDWFNNYGIRLLEPFSSTWFYGDTLFIIDIWILIALGIGLWRSLKAER
                     AGQALWARPAQIALVVMVTYVSLNMTISRMAVAEVRRIEPGVQIAVANSVPFAFWRRE
                     VLWRSANGFYGTIPCTVAACQRYPRVIRKTHWDDPRIAEWAKDDPAAQAFLFWSRMPL
                     AEPGPKGILLKDQRFKDSPARDNFTYLLKPR"
     gene            2657..4027
                     /locus_tag="VFV30_03545"
     CDS             2657..4027
                     /locus_tag="VFV30_03545"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX4847194.1"
                     /translation="MTQPTLVWLRRDLRLADHAALVAACAAGPVVPVYVLDDETAAHR
                     AMGGASRWWLHHSLAALDAALRARGARLILRRGKSDEVLAALAAECGAREVHALHHYE
                     PWWRNAERAVAKRLELYLHHGNYLAPPGSVLTGSGTPYKIYTPFWRALQQQLPPPPPL
                     PAPERIPAPETWPDSDPLESWGLLPTRPDWAGGLRAEWTPGEGGALDRLEAFLNRAAK
                     YEQRRNLPAVEGTSRLSPHLAFGEISPATVWHRTADLGGSTGTFLGELGWRDYAQNVI
                     LQMPGYGSTSAKPEFESFPWRTGPEAEADLAAWQQGRTGYPIVDAGMRQLWHTGWMHN
                     RVRMIVASFLIKHLLIDWRRGEQWFWDTLVDADYGQNTVNWQWSAGSGVDANMFVRIM
                     APLTQSPKFDAAEYIRQWVPELAHLPEAAIHDPPLPVRGYPRKLVEHAAGRARALAAW
                     DALKGK"
     gene            complement(4013..5170)
                     /gene="galK"
                     /locus_tag="VFV30_03550"
     CDS             complement(4013..5170)
                     /gene="galK"
                     /locus_tag="VFV30_03550"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX4847195.1"
                     /translation="MSALAEATLRGFAEAFGHAPDIATLAPGRVNLIGEHTDYNDGWV
                     LPMAIGVGTVVVARRLAEPELRIAALDLGQSATVRLDDPIPPAPHGDWSNYLRGVAQA
                     LREQGLELAGAELAIAGNVPQGAGLSSSAALEVAAGLALAALAGAPDHDRTLLAQAGQ
                     QAEHRYAGCQCGIMDQLVSARGEASAALLIDCRSLATHAVPIPADAAVLIVHSGVRRG
                     LVEGEYNQRRAQCEAAARHFAVPALRDLDAARLEAERDALDPTVYRRARHVVSENART
                     LAAAEALQAGDLARMGALMAQSHQSMREDFAITTPEIDHLRDLLAESLGAKGGARMTG
                     GGFGGAVVAVGARAAIAAAADLARDRYRTPDGAPPEIMIETACAGARLLAL"
     gene            complement(5167..6186)
                     /locus_tag="VFV30_03555"
     CDS             complement(5167..6186)
                     /locus_tag="VFV30_03555"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEX4847196.1"
                     /translation="MSFDPENHSHRRYDPLGDEWVLVSPHRAKRPWLGEEHSPAADRP
                     PAYDPECYLCPGNRRATGEVNPTYEGPFVFANDFPALQADVPTETEVSADPLFQRAPA
                     RGEARVICFSPDHARTLPDLYDAAIGAVVDCWCEQAAELGARYHWVQVFENKGAMMGC
                     SNPHPHGQIWATDTVPHLPATECRTQLAWWQDHGSALLRDLAEREAGGPRQVIATDGW
                     LALVPYWAKWPFEVLLLPRFVCQRLPDLDAAMRADLAHVLGRLTRAYDALFGVSFPYS
                     MGWHGAPFDGQDHPEWQLHAHFHPPLLRSASVRKFMVGYEMLAEAQRDLTPEQAAERL
                     RAVAG"
     gene            complement(6183..>6700)
                     /locus_tag="VFV30_03560"
     CDS             complement(6183..>6700)
                     /locus_tag="VFV30_03560"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX4847197.1"
                     /translation="QRGFLQFLPPEVMGSHVGTAPAHTTGRSQPLAFRAAVALQGHFG
                     VELDPERLDAAETDALRGWIGFYKDWRGHLHGSVWNGVTADGLVWHAAGTPHDWVLLV
                     YRTEPAAQPFPALLPLPFADPAARYAVARIGPDRRDPEAVFDGTWLARAGLALPPLHA
                     ERAVIYHGQAQ"
BASE COUNT         1074 a         2232 c         2344 g         1050 t
ORIGIN      
        1 gggcgatctt ctgaagggtt acgagcggat cctcgtgccc gaaatgaaca ccggccagtt
       61 caagaccgtg ctgcgcgacc agttcctggt cgacgccaag ccgtacaaca aggtgagcgg
      121 ccagccgttc accatcgccg agatcgaagc cgcgatcgaa ggggccctgg catgaacgcg
      181 atcacgccca tcaccaccac cctcaaggac tgggaatcgg atcaggaagt ccgctggtgc
      241 ccgggctgcg gggactatgc gatcctcaag gcggtgcagc gcaccctgcc ggaaatcggc
      301 gcggatccgg ccaagacggt gttcgtttcg ggcatcggct gctccagccg cttcccgtac
      361 tatgttgaaa gctatggctt ccacaccatc cacggccgcg cgccggcggt ggcgacgggg
      421 atcaagctgg ccaatccgga actggatatc tggctggtca ccggcgacgg cgacgggatg
      481 agcatcggcg gcaaccacac gatgcacctg ctgcgccgca acctgaactg ccaagtgctg
      541 ctgttcaaca acgagatcta cggcctgacc aagggccagt actcgccgac cagccgtgaa
      601 ggcacccaca gcccgaccac gccgctcggt tcggtggacc ggccggcgct gccctgcgcc
      661 ttcgcgctgg gggccggggc acggttcgtg gcgcgcgggt tcgacgtgtc aaagcatctg
      721 ccggacgtgc tgaaggcggc ccacgcccac aagggcgcgg ctttcgtgga aatcttccag
      781 aactgcatcg tctacaacaa ggacgtcttc aacgatttcg ccgcgcccaa gggcgccgaa
      841 gaccggcagc tgtggttgaa gaacggcgag ccgatgctgt tcgccggcgg ggcccagggc
      901 attgccctcg atgccgagaa gctgacgctg aaggttgtcg acgtggtgga cggcgattgg
      961 caggcggcgg gcgtgatcgt ccacgatgtc accaaccgct cgatcgcgca catgctggtc
     1021 gaaatgccgt tcggcccgtt cccgatggcc ttgggtgtga tctacgacga tccccgcccg
     1081 acctacgagg acgcggtgct gggccaggac accaaggccc gcgaaggcaa gagcaccgac
     1141 ctggcgcgcc tgctcgccaa gggccagacc tggacggtga gcgaggacgg gccggagctg
     1201 taagggcagg gcatctcaca cgaaggctcg aagtcatcgc atagcgctat ttgatcttcc
     1261 tcacgcgaag gcgcaaagac gcgaagatgt cgcgccgaga ggaaatcgaa gcccttgccc
     1321 ggaccgttgt cgattgcggt tttcagctgc atcgggacat cggtccggga ttgctcgagt
     1381 cggtctacca gactttgatg cttgccagca ttcaagagcg cggacttgcc gttgcgaaag
     1441 aggtggctgt tccgatcaag ttcaagggcg ttgtcatcga caacgccttc aaggcggacc
     1501 tgatcgttga agaccggttg ctgattgagc ttaaatcaac cgagcgagtg gcccccgttc
     1561 atgcgaagca ggttctgact tatctgcgct tgctcaacat gccgcttgga ttgctaatga
     1621 atttcggcca accaacgttc aaggacggcc tgcagcgggt ggtcaacaac catcactgac
     1681 gatgccatcc ggcaagacct cttcgcgcct ttgcgtcttt gcgtgagaca ttcatggaca
     1741 atatcaccca ttccttgatc ggggccgcgc tggggcaggc ggggctcaag cgcaagaccg
     1801 ggctggccat gccggcgctg atcatcggtg ccaacctgcc cgatatcgat gccggttgcg
     1861 tggtctatgg gatcgaaagc ctggccatgc gccgggggat cacccacggg ccgatcgccc
     1921 tggccgtgct gccgctgctg ctggcagggc tgctgtgggg ttatgatcgc tggcaggccg
     1981 ggcgaggcaa gcggcccgtg gggcggctgc cggtgcgctt cggctggctc ttggcgcttt
     2041 gctatctcgg cacgctcagc catccgctgt tcgactggtt caacaattac ggcattcgtc
     2101 tgctggagcc gttttccagc acctggttct atggcgatac gctgttcatc atcgacatct
     2161 ggatcctgat cgcactgggg atcgggctgt ggcggtcgct gaaggccgag cgggccggtc
     2221 aggcgctgtg ggcccgtccg gcccagatcg cgctggtggt gatggtcacc tacgtctccc
     2281 tcaacatgac gatcagccgg atggcggtgg ccgaagtgcg ccggatagag cccggggtgc
     2341 agatcgcggt ggccaactcc gtgccatttg ctttctggcg gcgcgaggtg ctgtggcggt
     2401 ccgccaacgg tttctacggc acgatcccct gcacggtggc ggcctgccag cgctatcccc
     2461 gggtgatccg caagacccat tgggacgatc cgcggatcgc cgaatgggcc aaggacgatc
     2521 cggcggcaca ggcctttctg ttctggagcc gcatgccgct ggccgaaccc ggaccaaagg
     2581 gcatcttgct gaaggaccag cgtttcaagg atagcccggc gcgagacaac tttacctacc
     2641 tgttgaaacc gcgctgatga cccaacccac gcttgtctgg ctgcgccgcg atctgcggct
     2701 ggccgatcat gccgccctgg tggccgcctg cgccgccgga ccggtggtgc cggtttatgt
     2761 gctggatgac gagacggcgg cgcaccgcgc gatgggcggg gcgagccgct ggtggctgca
     2821 ccattcgctg gccgcgctgg acgcggcgct gcgggccagg ggcgcgcgcc tgatcctgcg
     2881 gcgcgggaaa agcgacgagg tgctggccgc gctggcggcg gaatgcggcg cgcgcgaagt
     2941 ccacgcgctg caccattacg agccgtggtg gcgcaacgcc gagcgcgcgg tggccaagcg
     3001 gctggagctg tacctgcacc acggcaacta cctggccccg ccgggttcgg tgctgaccgg
     3061 atcgggcacc ccctacaaga tctacacccc gttctggcgc gcgttgcagc agcagttgcc
     3121 gccgccgccc ccgctgcccg cgccagagcg gatcccggca cccgagacct ggccggacag
     3181 cgatccgctg gaaagctggg gcctgctgcc cacccggccg gactgggcgg gcgggctgcg
     3241 cgcggaatgg acgccgggcg agggcggggc gctggaccgg ctggaggcct tcctgaaccg
     3301 cgccgcaaag tacgagcagc gccgcaacct gccggcggtg gaaggcacct cgcgcctctc
     3361 tccgcacctg gcctttggcg agatctcgcc cgcgacggtc tggcatcgca cggccgatct
     3421 gggcggatcg accggaacct tcctcggcga gctgggctgg cgcgactatg cccagaacgt
     3481 gatcctgcaa atgcctggct atggcagcac cagcgccaag ccggagtttg agagttttcc
     3541 ctggcgcacc gggcccgagg ccgaagcgga cctggccgcc tggcagcagg ggcgcacggg
     3601 ctatccgatt gtcgatgccg ggatgcgcca gctgtggcac accggctgga tgcacaaccg
     3661 cgtgcggatg atcgttgcca gcttcctgat caagcatctg ctgatcgatt ggcggcgcgg
     3721 cgagcaatgg ttctgggaca ccctggtcga tgccgattac gggcaaaaca cggtcaactg
     3781 gcagtggagc gcgggatcgg gcgtggacgc caacatgttc gtgcggatca tggcgccgct
     3841 gacccagtcg cccaagttcg atgccgccga gtacatccgg caatgggtgc cggaactggc
     3901 ccatctgccc gaagcggcaa tccacgatcc gccgctgccg gtgcgcggtt acccgcgcaa
     3961 gctggtggag cacgccgcgg gccgggcccg ggcgctggcg gcctgggacg cgctaaaggg
     4021 caagtagccg cgccccggcg caggccgttt cgatcatgat ctccggcggc gcgccatcgg
     4081 gtgtgcggta gcggtcacgg gcaaggtcgg ccgccgcggc gatcgcggcg cgcgcgccga
     4141 cagccaccac cgcgccgcca aagccgccgc cggtcatccg cgcgccgccc tttgcgccaa
     4201 ggctttcggc cagaaggtcg cgcaggtggt cgatctcggg cgtggtgatg gcaaaatcct
     4261 cgcgcatgga ctggtgcgat tgcgccatca gcgcgcccat ccgggccaag tcgccggcct
     4321 gcagcgcctc ggccgcggcc agggtgcggg cattctcgct caccacgtgg cgggcgcggc
     4381 ggtagacggt gggatcgagc gcatcgcgct ccgcctccag ccgggccgcg tccaaatcgc
     4441 gcagggcggg caccgcaaag tgccgggccg ccgcctcgca ctgggcgcgg cgctggttgt
     4501 attcgccttc gaccagtccg cgccgaaccc cggaatggac gatcagcacg gccgcatctg
     4561 ccgggatcgg cacggcgtgg gttgccaggc tgcggcagtc gatcagcagc gccgcgctgg
     4621 cttcgccgcg ggccgacacc agctggtcca tgatcccgca ctggcacccg gcatagcggt
     4681 gctcggcctg ctgcccggcc tgggccagca gggtgcggtc gtgatcgggc gcgccggcca
     4741 atgcggcaag cgccagcccg gcggccactt ccagcgcggc ggaggatgac aggccggcgc
     4801 cttgcgggac gttgccggca atcgccagct ccgccccggc cagctccagt ccctgttcgc
     4861 gcagcgcctg cgccacgccg cgcaggtagt tcgaccagtc cccgtgcggt gccggcggga
     4921 tcggatcgtc cagccgaacg gtcgcgcttt gccccaggtc cagcgcggcg atgcgcagtt
     4981 ccggctcggc cagccgccgg gcgaccacca ccgtgccaac cccgatcgcc atcggcagga
     5041 cccagccgtc gttgtagtcg gtgtgctcgc cgatcaggtt gacccgtccg ggggcgaggg
     5101 tggcaatatc gggcgcgtgg ccaaaggcct cggcaaagcc gcgcagggtc gcttcggcca
     5161 gcgcgctcat cccgccaccg cccgcagccg ctcggccgcc tgctccgggg tcagatcgcg
     5221 ctgcgcctcg gccagcatct catagccgac catgaacttg cgcacgctgg cagagcgcag
     5281 cagtggcggg tggaaatggg cgtgaagctg ccattccgga tggtcctggc cgtcgaacgg
     5341 cgcgccgtgc caacccatcg agtagggaaa gctgacgccg aacagcgcat cataggcgcg
     5401 cgtcagccgg cccagtacat gcgccagatc ggcccgcatg gcggcgtcca gatcgggcag
     5461 gcgctggcag acgaagcggg gcagcagcag cacttcgaac ggccacttgg cccaataggg
     5521 caccagtgcc agccagccat ccgtcgcgat cacctggcgc ggcccgccgg cctcgcgttc
     5581 ggccaggtcc cgcagcaggg cggagccgtg gtcctgccac caggccagct gggtgcggca
     5641 ttcggtggcg gggaggtggg ggacggtgtc ggtagcccag atctggccgt gcggatgggg
     5701 gttggaacag cccatcatcg cgcctttgtt ctcgaacacc tggacccagt ggtagcgcgc
     5761 gcccagttcg gcggcctgct cgcaccagca gtccaccacc gcgccgatcg cggcatcgta
     5821 aaggtcgggc agggtgcggg cgtggtccgg cgagaagcag atcacccgcg cctcgccccg
     5881 ggccggcgcg cgctggaaca gcggatcggc gctgacttca gtctcggtcg gcacatcggc
     5941 ctgcaaggcc ggaaaatcgt tggcgaaaac gaacggccct tcgtaggtcg ggttcacttc
     6001 cccggtcgcg cggcggttgc cggggcacag gtagcactcc ggatcatagg ccggcggacg
     6061 gtccgcggcg ggcgaatgct cctcgcccag ccaggggcgc ttggcccggt gcggggatac
     6121 cagcacccat tcgtcgccca gcggatcgta gcgccggtgg gaatggtttt cggggtcaaa
     6181 actcattggg cctgtccgtg atagatcact gcccgctcgg cgtggagcgg gggcagggcc
     6241 aggccggcgc gggccagcca ggtgccgtcg aacaccgctt cgggatcgcg ccggtctggt
     6301 ccgatccggg ccacggcata gcgcgccgcc ggatcggcaa agggcagggg cagcagcgcc
     6361 gggaagggct gggccgccgg ctcggtgcgg tagaccagca ggacccagtc gtgcggcgtc
     6421 ccggccgcgt gccagaccag cccgtccgcc gtcacgccgt tccagaccga gccgtgcagg
     6481 tgtccgcgcc agtccttgta gaagccgatc cagccgcgca gcgcatcggt ctcggccgcg
     6541 tcgagccgtt cggggtccag ttccaccccg aagtggcctt gcagcgccac ggccgcgcgg
     6601 aaggccagcg gctggctgcg cccggtggtg tgggccgggg cggtgccgac gtgggatccc
     6661 atcacttcgg ggggcaggaa ttgcaggaaa ccgcgctgga
//
LOCUS       DASUDV010000169         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2126048,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000169 DASUDV010000000
VERSION     DASUDV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126048"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFV30_03565"
     CDS             <1..385
                     /locus_tag="VFV30_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847198.1"
                     /translation="FVTLPTSAAPAELIGCVGLGEVEGEVELGYWFARPHWGKGYATE
                     AARAVLRLAKVLGHREIVAGHFIDNPASGKVLRKVGFVPTGRIRHRYSLARGYDAQSV
                     EHRIALGRPSDCDGDADPAAAMRAA"
     gene            complement(448..1365)
                     /locus_tag="VFV30_03570"
     CDS             complement(448..1365)
                     /locus_tag="VFV30_03570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX4847199.1"
                     /translation="MNAPTKFPLDVEPAAPLGKLDLANSGPTPADLEIVVRDQRFGRE
                     QKPGRWWLGGDPVATAWHNALSATFPRGEAMFIEAVKAHREGAPPKLEAEIRAFVKQE
                     INHTREHVVFNKAAADAGYDMAKIDARVAELMEMIKQRPQIVNLAATIALEHYTAMMA
                     HEFLANPQHFKNAEPETAAMWRWHAIEEIEHKGVAYDTFLHATRDWSRLRRYRLKSVM
                     MLLVTFNFLRNRWNDTLELLEQDGLTGWKVKGKLAWYLVGTPGVLRKIFPAWVSYFLP
                     GFHPWKHDDRALIAKAESEFPDAVMVPAE"
     gene            1394..1993
                     /locus_tag="VFV30_03575"
     CDS             1394..1993
                     /locus_tag="VFV30_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847200.1"
                     /translation="MSIRKRLTQEESRTVALEAARALLIELGPQAVTLKAVAARIGRT
                     HANLLHHFGSAAGLQKALAEHLAVTICATIEEAVLASRAGEGSPREVVDLTFDAFDKE
                     GGGALATWMLMNGNEDALDPLLEVIHELVDDLGEHEGGKMHDVTHALCLMALGDALMG
                     GPLAQSLGLKRTAARDIAEKMLIEGAIAAGIVVPGQPSS"
     gene            complement(1978..2691)
                     /locus_tag="VFV30_03580"
     CDS             complement(1978..2691)
                     /locus_tag="VFV30_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX4847201.1"
                     /translation="MTATHLTSRAVIRLTALEEGEDIRAFLNGLVTNDVTGALPVWSA
                     LLSPQGKVLFDFIVWDGGADLLLDCEANQAEALIKRLSLYRLRRKIGLAPDPALGVHW
                     QASEGASPDPRLPALGQRWLGAPEGESADDPWLAHRLSHGVCEGAGELADLLWLECNA
                     AELNGVSFTKGCYVGQENTARMNWRQKVNRRLVVVPLAESDPARQRAAYPELGLAVDH
                     RRVEDIRAEWRPEWLQLDG"
BASE COUNT          438 a          909 c          965 g          443 t
ORIGIN      
        1 cttcgtcacc ctgccgacca gcgcggcccc ggccgaactg atcggctgtg tcgggctggg
       61 cgaagtcgag ggcgaggtgg agctgggcta ctggttcgcc cgtccgcact ggggcaaggg
      121 ctatgccacc gaggcggccc gcgccgtgct gcggctggcc aaggtgctgg gccaccgcga
      181 gatcgtggcc ggccacttca tcgacaatcc ggcctcgggc aaggtcctgc gcaaggtcgg
      241 cttcgtgccg accgggcgga tccgccaccg ctacagcctg gcccgcggct atgacgcgca
      301 gtcggtcgaa caccggatcg cgctgggccg gccgagcgat tgcgacggcg acgccgatcc
      361 ggccgccgcg atgcgagcgg cctgaacaag aaaaggggcc ccgaccggct ggtcggggcc
      421 ccgttcgttt gcggctatgg cgccggccta ttcggcggga accatcaccg catccggaaa
      481 ttcgctttcg gccttggcaa tcagcgcgcg gtcgtcgtgc ttccaggggt ggaagccggg
      541 caggaaatag ctgacccagg ccgggaagat cttgcgcagc acgcccggcg tgccgaccag
      601 gtaccaggcc agcttgccct tgaccttcca gccggtcagc ccgtcctgct ccagcaattc
      661 cagcgtgtcg ttccagcggt tgcgcaggaa gttgaaggtc accagcagca tcatcaccga
      721 cttcaggcgg taacggcgca ggcggctcca gtcgcgggtg gcgtggagga aggtgtcata
      781 ggcgacgccc ttgtgctcga tctcctcgat cgcgtgccag cgccacatcg ccgccgtttc
      841 cggctcggcg ttcttgaaat gctgcgggtt ggcgaggaat tcgtgcgcca tcatcgcggt
      901 gtagtgttcc agcgcgatcg tcgcggccag gttcacgatc tgcgggcgct gcttgatcat
      961 ttccatcagc tcggccaccc gggcgtcgat cttcgccatg tcgtaaccgg cgtcggccgc
     1021 ggccttgttg aacaccacgt gctcgcgggt gtggttgatt tcctgtttca cgaaggcgcg
     1081 gatttcggcc tccagcttgg gcggggcgcc ctcgcggtgg gccttcacgg cctcgatgaa
     1141 catcgcttcg ccgcgcggga aggtggcgga cagggcgttg tgccaggcgg tggcgaccgg
     1201 atcgccgccc agccaccagc ggccgggctt ctgctcacgg ccaaagcgct ggtcacggac
     1261 cacgatctcc agatcggcgg gggttgggcc agaattggca aggtccagct tgccgagggg
     1321 cgcggccggt tctacgtcga gggggaattt ggtgggggcg ttcatgacgc ctgtatgggg
     1381 ataacttaca ctgatgtcaa taaggaagcg cctgacccag gaagaaagcc gcaccgtggc
     1441 gctggaagcc gcgcgcgcgc tgctgatcga gctgggtccg caggccgtca cgctgaaggc
     1501 ggtggcggcg cgaatcggcc gcactcacgc caacctgctg caccatttcg gatcggccgc
     1561 cgggctgcag aaggccctgg ccgaacatct ggccgtgacc atttgcgcca cgatcgaaga
     1621 ggccgtgctg gccagccgcg cgggcgaagg aagcccgcgc gaggtggtgg acctgacctt
     1681 cgatgccttt gacaaggaag gcggcggggc gctggccacc tggatgctga tgaacggcaa
     1741 cgaggacgcg ctcgacccgc tgctggaggt gatccacgaa ctggtcgacg acctgggcga
     1801 gcacgaaggc ggcaagatgc acgacgtcac ccacgcgctg tgcctgatgg cgctgggcga
     1861 tgcgctgatg ggcgggccgc tggcccagtc gctcggcctg aagcgcaccg cggcgcgcga
     1921 cattgccgaa aagatgctga tcgaaggcgc gatcgccgcc gggatcgtgg tgccgggtca
     1981 gccgtccagc tgaagccatt ccggtcgcca ttcggcccgg atatcctcaa cccggcggtg
     2041 atcgaccgcc agccccagct ccggataggc cgcgcgctgg cgggcgggat cgctctccgc
     2101 cagcggcacc accaccagcc gccggttgac cttctgccgc cagttcatcc gggcggtgtt
     2161 ctcctgcccg acataacagc ccttggtaaa gctgacgccg ttcagctcgg ctgcgttgca
     2221 ttccagccag agcaggtcgg ccagctcgcc cgcgccttcg cagacgccgt gcgaaagccg
     2281 gtgggcaagc cacggatcgt ccgcgctttc tccctccggt gcgccaagcc agcgctggcc
     2341 gagcgcgggc aggcgggggt cgggggaggc cccctcgctg gcctgccagt gcacccccag
     2401 cgcgggatcg ggggcaaggc cgatcttgcg gcgcaggcgg tagagcgaga gccgcttgat
     2461 cagcgcctcg gcctggttcg cctcgcaatc gagcagcagg tctgcgccgc cgtcccacac
     2521 gatgaaatcg aacagcacct tcccctgggg gctgagcaag gccgaccaga ccggcagcgc
     2581 gccggtgaca tcgttggtca ccagcccgtt caggaacgcg cggatgtcct ccccctcctc
     2641 aagagcggtc agacggatca cggcgcggct ggtcagatgg gtggcggtca tgggtgacag
     2701 atagggtgcc tgccgcttaa gggaaaggca tgaccgagac cccgaatcgc ctgac
//
LOCUS       DASUDV010000170         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_977466,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000170 DASUDV010000000
VERSION     DASUDV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977466"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="VFV30_03585"
     CDS             complement(<1..1450)
                     /locus_tag="VFV30_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX4847202.1"
                     /translation="MGGARAENWTAAAEALASGRSSLIWRKLVADCETPVGAAVKLIE
                     PGRGDFLLESVEGGEVRGRYSLLGLDPDLVFRATGAAAEVNRAWRHDRAAFTPLAGDS
                     LSELRQLVAACRIDVPPALPPVLACLVGYFGYETYGLVEDLPRPAENPLALPDMLFVR
                     PALILVFDRLSDALWCVAPVWPDGGQAEHLLEAAQDRIDAALQGLQAPVPAPVRDASL
                     PEPVLTPTMAPGQYRDMVLRAKDYIEAGDIFQVVLAQRFTSPFTLPPLALYRALRRVN
                     PSPFLYFLDLPGFALTGSSPEILVRVRNGEVTIRPIAGTRPRGASPEEDRANEASLLA
                     DPKERAEHLMLLDLGRNDVGRVAEAGTVKVTESYTIERYSHVMHIVSNVVGRLDGANH
                     DAIDAVFAGFPAGTVSGAPKVRACEIIAELEPETRGAYAGGVGYFAPDGNLDSCIVLR
                     TGVVKDGMLHVQAGAGIVADSDPAYEQRECEAK"
     gene            complement(1461..>2513)
                     /locus_tag="VFV30_03590"
     CDS             complement(1461..>2513)
                     /locus_tag="VFV30_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4847203.1"
                     /translation="AGGKTLEAAAGPKGLAVAQLGTLDRGELALQASSQAAEAVFATG
                     TGKMAGPFKATIGWVLIRVDGVEKTPGKTLEQARPELVKELGEEKRREAIAEFSARIE
                     DMIVNGASLTDVAKDLNLTIVETPALTANGEVYGKPGVTAPKEVAPVLQAAFAMDGEK
                     QPQLAETDPGKAFMIFDVGTLAPAAPPPLAEIRDTVIADVQLSKGAKLAKAAAEKVKA
                     QIEKGVPPEVAIASLGVALPPVDRFDRPRLEVEAQGRNAPRPLLLGFVMAKGKVRMMA
                     APRNRGWYVVTVTEVIPGTVDPKDARLAGLQNDLVATTGEEYAQQLRTGMGNEVGVTR
                     NEGNIGKLKQRLEAGQ"
BASE COUNT          352 a          870 c          881 g          410 t
ORIGIN      
        1 ccttggcctc gcattcgcgc tgttcatagg cggggtcgct gtcggctacg atgccggccc
       61 cggcctgcac gtggagcatg ccgtccttga ccaccccggt gcgcagcacg atgcagctgt
      121 caaggttgcc gtcgggcgcg aaatagccga ccccgccggc ataggcgccg cgggtttcgg
      181 gctccaactc ggcaatgatc tcgcaggcgc gaaccttggg cgcgccgctg accgtgccgg
      241 cgggaaagcc cgcgaagacc gcgtcgatcg catcgtgatt ggccccgtcg agtcgcccga
      301 ccacgttcga gacgatgtgc ataacgtgac tgtagcgctc gatcgtatag ctctcggtca
      361 ccttgacggt cccggcctcg gccacccggc caacgtcgtt gcggccgaga tcgagcagca
      421 tcaggtgctc ggcccgttcc ttgggatcgg ccagcaggct cgcctcgttg gcgcggtctt
      481 cctcgggcga ggcgccgcgc gggcgggtgc cggcgatcgg ccggatcgtc acttcgccgt
      541 tgcggacccg gaccaggatc tcgggactcg atccggtcag ggcaaagccc ggcagatcga
      601 ggaaatagag gaacggcgag ggattgaccc gccgcagcgc gcggtagagc gccagcgggg
      661 gcagggtgaa ggggctggtg aagcgctggg ccagcaccac ctggaagatg tcgccggcct
      721 cgatgtagtc cttcgcgcgc agcaccatgt cgcggtactg gccgggggcc atggtcgggg
      781 tcagcaccgg ttcgggcagg ctggcatcgc ggaccggggc cggcaccggt gcctgcaaac
      841 cctgcagcgc ggcgtcgatc cggtcctgcg cggcttcgag caggtgttcc gcctgaccgc
      901 catcgggcca gaccggggcg acgcaccaca gcgcgtcgct cagccggtcg aacaccagga
      961 tcagcgcggg ccgcacaaac agcatgtcgg gcagggccag cgggttctcc gccgggcgcg
     1021 gcaggtcttc gaccaggcca taggtttcat agccgaaata gccgaccaga caggccagca
     1081 ccggcggcag tgccggcggg acgtcgatcc ggcaggccgc gaccagttgg cgcagttcgc
     1141 tgaggctgtc gccggccagc ggcgtaaacg cggcgcggtc gtgccgccag gcgcggttga
     1201 cttcggccgc ggcgccggtg gcccggaaga ccagatcggg atcaagcccc agcaggctgt
     1261 agcgcccgcg cacttcgccg ccttcgacgc tttccagcag gaaatcgccc cggcccggtt
     1321 cgatcagctt gaccgcggcc ccgaccgggg tctcgcagtc ggccaccagc ttgcgccaga
     1381 tcaggctgga ccggcccgag gcgagggcct cggcggctgc cgtccagttc tccgcccgtg
     1441 cgccgcccat cgcctgccgc ttactggccg gcctcaagcc gctgcttgag cttgccgatg
     1501 ttgccttcgt tgcgggtcac gcccacttcg ttgcccatgc cggtgcgcag ctgctgggcg
     1561 tattcctcgc cggtcgtggc gaccaggtcg ttctgcagcc cggccaagcg cgcatccttg
     1621 gggtcgacgg ttcccgggat cacctcggtc acggtgacga cataccagcc gcggttgcgc
     1681 ggtgcggcca tcatccggac cttgcccttg gccatcacga agcccagcag cagcggacgg
     1741 ggcgcgttgc ggccctgggc ctcgacttcg agacggggac ggtcgaaccg gtcaaccggg
     1801 gggagggcga cacccagcga ggcgatcgcc acttccggcg gtacaccctt ttcgatctgc
     1861 gccttgacct tttcggccgc ggccttggcc aacttggcgc ccttcgacag ctggacatcg
     1921 gcgatcacgg tgtcgcggat ctcggccagc ggcggcggcg cggccggagc gagggtgccg
     1981 acgtcgaaga tcatgaaggc cttgccggga tcggtttcgg ccagctgcgg ctgcttttcg
     2041 ccgtccatcg cgaaggcggc ctgcagcacc ggagcaacct ccttgggagc ggttacgccg
     2101 ggcttgccat agacttcgcc gttggcagtc agcgccgggg tttcgacgat ggtcaggttc
     2161 agatccttgg ccacgtcggt caggctggcg ccgttgacga tcatgtcctc gatccgggcg
     2221 gagaattcgg caatcgcctc gcggcgcttt tcctcgccca gttccttgac cagttcgggg
     2281 cgggcctgct ccagcgtctt gcccggggtc ttctccaccc cgtcaacgcg gatcagcacc
     2341 cagccgatcg tcgccttgaa cgggccggcc atcttgccgg tgccggtcgc gaagaccgct
     2401 tcggcggcct ggctggaggc ctgcagggcc agctccccgc ggtcgagcgt gccgagctgg
     2461 gcaacggcca gccccttggg gccggcggct gcttcgaggg tcttgccccc ggc
//
LOCUS       DASUDV010000171         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1949276,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000171 DASUDV010000000
VERSION     DASUDV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949276"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFV30_03595"
     CDS             <1..751
                     /locus_tag="VFV30_03595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4847204.1"
                     /translation="YIPDLKGSAYDDVTVRQLLTMSSGLAWNEDYEDPKADVAKFNNA
                     EPDPGVDATVSYLRKLPRAHAPGAVWHYNTGETNLIGVLVSSAVKKPLSQYLEEKIWK
                     PAGMEAKATWLLGKTGHEIAGCCIQAATRDYARFGLFVLANGKGVNGEQIVPEDWFAQ
                     AIVKQKDIGEPGHGYGFQWWTYDDGSVAAQGIFGQGIFIDPKRRLVIASNANWTRATL
                     GPEGDAREAFYKQVQALIDAEAKAKSAQGQS"
     gene            complement(736..1326)
                     /locus_tag="VFV30_03600"
     CDS             complement(736..1326)
                     /locus_tag="VFV30_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847205.1"
                     /translation="MTLASGTDKLESERLVLRRITRDDLPFYSRIQADPDVARYIATG
                     KPRSAEESLGWLEAVLASYASVGLGQLAVVRKADGVLVGRCGLSDSVVETDPAPGAVR
                     RGWFFRAQAPEDVGTEPVPELGYTFAREHWGHGYASEAAACVHAYARAVLPVGKIMSV
                     IHPDNHASVGVVRKYGVRLVEKIEMSTQQFDRYDWP"
     gene            complement(1354..1774)
                     /gene="rnpB"
                     /locus_tag="VFV30_03605"
     ncRNA           complement(1354..1774)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFV30_03605"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1783..2484)
                     /locus_tag="VFV30_03610"
     CDS             complement(1783..2484)
                     /locus_tag="VFV30_03610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX4847206.1"
                     /translation="MSELIHYETPSPNWNERQSPVSMVVLHYTGMQTAEEALARLCDE
                     AAQVSAHYLIEEDGTVHRLVREDRRAWHAGKSYWRGVTDVNSASVGIELVNPGHEFGY
                     RPFPDAQMEALLPLLAEIVQRHDIPRANVVGHSDVAPARKEDPGELFDWDLLAAHRLA
                     LKTPRVSMPSPFENDGAFFLALERWGYDISDPAAAMRAFQRRWRPHIIDGVPDGECSA
                     ILFSLLLDRDSGKTR"
     gene            complement(2489..>3055)
                     /locus_tag="VFV30_03615"
     CDS             complement(2489..>3055)
                     /locus_tag="VFV30_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX4847207.1"
                     /translation="ALTNGLELLADEKDPEMRKRCFELLEQSAKISADKLKFFRLAFG
                     AAGGFGESVAVEEAKSLVDALAANNARVTVNWALGVATLPKPAIKVLLNFALLGMEAL
                     VRGGTLDIGAESRGGASEIVVRASGPRIAFDQEIGKALEGELGEGGLSSRNAPAFMLY
                     QIAARQGGGLQFALTDDALVLGAVLPEG"
BASE COUNT          530 a         1023 c          987 g          515 t
ORIGIN      
        1 ctatatcccc gatctcaagg gttcggccta tgacgatgtc acggtgcgcc agttgctgac
       61 gatgagttcg ggtctggcct ggaacgagga ttatgaagat cccaaggccg atgtcgcgaa
      121 attcaacaac gccgagcccg atcccggggt cgatgccacg gtcagctatc tgcgcaagct
      181 tccccgtgcg catgcgccgg gcgcggtgtg gcactacaac accggcgaaa ccaacctgat
      241 cggggtgctg gtaagttccg cagtcaagaa gccgctcagc cagtatctcg aagaaaagat
      301 ctggaaaccg gccggcatgg aagccaaggc gacctggctg ctgggcaaga ccgggcacga
      361 gatcgccggg tgctgcatcc aggccgcgac gcgcgactat gcccgcttcg ggctgttcgt
      421 gctggccaac ggcaagggcg tgaacggcga gcagatcgtg cccgaagact ggttcgccca
      481 ggcgatcgtc aagcagaagg acatcggcga accgggccac ggctatggct ttcagtggtg
      541 gacttatgac gatggctcgg tcgccgccca ggggatcttc ggccagggga tcttcatcga
      601 tcccaagcgc cgcctggtga tcgccagcaa cgccaactgg acccgcgcga cgctgggccc
      661 cgaaggcgat gcgcgcgagg cgttctacaa gcaggtccag gcgctgatcg atgcggaggc
      721 gaaagccaag agcgctcagg gccagtcgta gcgatcaaac tgctgggtcg acatttcgat
      781 cttttcgacc agcctcaccc cgtatttgcg caccacgcca accgaggcgt ggttatcggg
      841 gtggatcacc gacatgatct tgcccaccgg cagcacggcg cgggcatagg cgtggacaca
      901 ggccgccgct tcgctggcat agccgtgtcc ccaatgttcg cgcgcgaagg tatagcccag
      961 ttcgggcacc ggctcggtcc ccacgtcctc aggcgcctgg gcgcggaaga accagccccg
     1021 gcgcaccgca cccggcgcgg gatcggtctc gaccaccgaa tcgctaagcc cgcagcgccc
     1081 caccagcacg ccatcggcct tgcgcaccac cgccagctgg ccgagaccca ccgaggcata
     1141 gctggccagc accgcttcaa gccaacccag cgattcctcg gccgagcgcg gcttgccggt
     1201 ggcgatatag cgggccacat cgggatcggc ctggatccgg ctgtagaagg gcagatcatc
     1261 gcgcgtgatc cggcgcagca ccaaccgctc gctctcgagt ttgtcggtac cgcttgccag
     1321 ggtcatcgct tgaccgtctc cttgatgaaa tcgttcgcca gggggcctgt aagccgggtt
     1381 ctgtcccgcc gacggtatgc gaaagcgcac cgtatcggcg ggggcagcca ttcctctcgg
     1441 cgccatgttg ccatggcgct ccagcaacca acccgggcgg tcgggctcga aacaagcctg
     1501 caccgaagtg cgcgccgccc ctattcggtc ttgctccggg tggggtttgc cgtgcgagcc
     1561 ctgttaccag ggccccggtg cgctcttacc gcaccctttc acccttacca gtaactggcg
     1621 gtctgctctc tgtggcactt tccctggggt tacccccggc gggcattacc cgccaccctc
     1681 gtttcgtgga gcccggactt tcctcgactg tcccgcttga gcggagagcg atccccgcct
     1741 tcgcggggac aggcgcggct gcccggcccc ctggcgaagg gcctatctag tctttccgct
     1801 gtcgcgatcc aacaacaaag agaacagaat cgccgaacat tcgccatcgg gcaccccatc
     1861 gatgatgtgc gggcgccaac gtcgctggaa ggcacgcatc gcggcggcgg ggtcggagat
     1921 gtcatagccc cagcgctcga gcgccaggaa gaacgccccg tcgttctcga acggtgaggg
     1981 catcgacacc cgcggggtct tgagcgccag gcggtgcgcg gcgagcaggt cccagtcgaa
     2041 cagttcgccc ggatcctcct tgcgcgccgg ggcgacatcg gaatggccga ccacgttggc
     2101 gcggggaatg tcgtggcgct ggacgatttc ggccagcagc ggcagcaggg cttccatctg
     2161 cgcatcgggg aacgggcgat agccgaattc gtgcccggga ttgaccagct cgatcccgac
     2221 gctggccgaa ttgacgtcgg tcaccccgcg ccaatagctt ttgccggcgt gccaggcgcg
     2281 gcgatcctcg cgcaccaggc ggtggaccgt gccgtcttcc tcgatcaggt aatgggccga
     2341 gacctgcgcc gcctcatcgc acagccgggc gagcgcctcc tcggcggtct gcatgccggt
     2401 gtagtggagc accaccatcg agacgggcga ttgccgctcg ttccaattgg gcgagggcgt
     2461 ctcgtagtgg atcagctcgc tcaagccgtc agccttcggg caggaccgcg ccgagcacca
     2521 acgcgtcgtc ggtaagggca aattgcagcc ccccgccctg ccgcgcggcg atctggtaaa
     2581 gcatgaaggc cggggcgttg cggctggaga ggccgccctc gcccagttcg ccttccagcg
     2641 ccttgccgat ttcctggtcg aacgcaatgc gcgggccaga ggcgcggacg acgatttcgc
     2701 tcgccccgcc gcggctttcc gcaccgatat cgagtgtgcc gccgcgcacc agggcttcca
     2761 ttccgagcag cgcaaagttc agcagcacct tgatcgccgg cttgggcagc gtggcgacgc
     2821 caagcgccca gttgacggtg acgcgggcgt tgttggcagc gagcgcatcg acgagcgact
     2881 tggcctcttc caccgcgacg ctttcaccaa agccgccggc agcgccgaag gcgaggcgga
     2941 agaacttgag cttgtcggcg ctgatcttcg cgctctgttc gagcagttcg aagcagcgct
     3001 tgcgcatttc cgggtccttc tcatccgcga gaagttccag gccattggta agcgc
//
LOCUS       DASUDV010000172         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1684445,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000172 DASUDV010000000
VERSION     DASUDV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684445"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFV30_03620"
     CDS             <1..434
                     /locus_tag="VFV30_03620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX4847208.1"
                     /translation="EAFAQAGADIITFHPEAGPHAHRTVQAIKALGKQAGVSLNPATP
                     AKMLDYLYEEVDLILVMSVNPGFGGQSFITSQLRKIEAIRKNIDKTGRDIRLEVDGGV
                     DAETARLCVAAGADVLVAGTATFRGGPERYAANIAALKGLG"
     gene            438..>2302
                     /locus_tag="VFV30_03625"
     CDS             438..>2302
                     /locus_tag="VFV30_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX4847209.1"
                     /translation="MVDVLSSRQDAQAAGRHDGPAIPLGTGAEEGVLSTADAPSAAPV
                     DGEVIEPGRALALADFAPPAIGAGERLIRLAYRLGVPASALTAPFGKTARPRLLATVN
                     NPLPGSKVAGTALRAGHFLVHGVKSPIAQIDFAGAARMTPPLERVVHSFSWLADLEAS
                     APREAAAPVAERILAQWLAANPKVPARPGKGPAWSVANAGHRELNWLVHAPLILSGGD
                     KALRARVLASLSETARWLDKNVGRAEDLLGEVAGWCGIVAAGLLLPDGKPRRLFGEAG
                     LVRALGELVGDDGGVLSRAPLGQIEAIALLVRLRACYQAVRRDPPQALEAIVALLVPP
                     LLALTHGDGSLGSWQGAWAVDGADLSALVAASGVRTRPLRDVRQWGYQRVVAQKGILQ
                     FDAAPPPLAKHARSGCASTLAFEFSHAGQRIVVNCGGAACAGGLMPVRLEQGLRASAA
                     HSTLVLDDANSTAVVINGKLGGGVSEVEVDRRTLVGEKLAGATRLEASHNGYASRYGL
                     THRRILILRDDGSELRGEDLLVPAARKGKNGKVGFAIRFHLGPGVEVRLSEDGQGAGL
                     ALPDGSYWQFRAGGGQLGIEESIWADGQGRPVPIHQLVIHGLVSRGGGNFAWLLK"
BASE COUNT          332 a          750 c          836 g          384 t
ORIGIN      
        1 tggaagcctt tgcccaggcc ggggccgata tcatcacctt ccatcccgaa gccggcccgc
       61 acgctcaccg aaccgttcag gcgatcaagg cgctgggcaa gcaggccggg gtctcgctca
      121 acccggcaac gccggccaag atgctcgact atctctatga agaggtcgac ctgatcctgg
      181 tgatgagcgt gaacccgggc tttggcgggc agagcttcat caccagccag ttgcgcaaga
      241 tcgaggcgat caggaaaaac atcgacaaga ccggccgcga tatccggctc gaggtcgatg
      301 gcggggtgga tgccgagacc gcgcgattgt gcgttgctgc cggtgccgac gtgctggttg
      361 ccggcacggc caccttccgc ggcgggccgg aacgctatgc cgccaatatc gccgccctga
      421 agggcctggg atgagcaatg gtggacgtgc tttcttctcg ccaggatgca caggcggcag
      481 ggcggcacga cgggccggcc attccgcttg gaaccggggc tgaggagggc gtcttgagca
      541 cagccgatgc gccttcggca gctcctgtcg atggcgaggt gatcgagccc ggtcgcgccc
      601 tggcgctggc cgatttcgca ccgcccgcga tcggtgcggg cgaacggctg atccgcctgg
      661 cctatcggct tggcgtgccg gcctcggcgc tgaccgcacc ttttggcaag actgccaggc
      721 cgcgcctcct ggcaacggtc aacaacccgc tgccgggcag caaggtggcc ggaaccgcgc
      781 tgcgtgcggg ccattttctg gtccatgggg tcaagtcgcc gattgcccag atcgatttcg
      841 ccggcgcggc gcggatgacc ccgccgctcg aacgggtggt ccattccttt tcctggctgg
      901 ccgatcttga agccagcgcc ccgcgcgagg cggcggcccc ggtggccgaa cggatcctgg
      961 cccagtggct ggccgccaat cccaaggtgc cggctcgccc cggcaagggg ccggcctgga
     1021 gcgtcgccaa tgccgggcac cgcgagctca actggctggt tcatgccccg ctgatccttt
     1081 ctggcggcga caaggccctt cgcgcaaggg tgctggccag cctctcggaa accgcgcgct
     1141 ggctcgacaa gaatgtcggc cgcgccgaag atctgctggg cgaagtcgcg ggctggtgcg
     1201 ggatcgttgc cgccggcctg ctgctgcccg atggcaagcc gcgccggctg ttcggtgaag
     1261 ccgggctggt acgggcgctg ggcgaactgg ttggcgatga tggcggggtg ctgtcgcgcg
     1321 cgccgcttgg ccagatcgag gcgatcgcct tgctggtccg ccttcgggcc tgctaccagg
     1381 cggtccggcg cgatccgccc caggcgctgg aagcgatcgt ggcgctgctg gtgccgccct
     1441 tgctggcgct cacccatggc gatggttcgc tgggttcgtg gcaaggagcc tgggcggttg
     1501 acggggctga cctgtcggcg ctggttgcag catcgggagt gcgcacccgc ccgctgcgcg
     1561 acgtgcgcca gtggggttat cagcgcgtcg ttgcgcaaaa gggcattctc cagttcgatg
     1621 ccgctccgcc gccgctggcc aagcacgcgc ggtcgggctg cgcatcgacc ctggcgttcg
     1681 aattcagcca tgccgggcag cggatcgtgg tcaattgcgg gggtgcggcc tgcgccggcg
     1741 ggctgatgcc ggtgcggctc gagcagggcc tgcgcgccag cgccgctcat tcgaccctgg
     1801 tgctcgacga tgccaattcg accgcggtgg tgatcaacgg caagctcggg ggcggggtct
     1861 cggaggtcga ggtcgaccgg cgcaccctgg tgggcgaaaa gctggccggg gctacccggc
     1921 tcgaggccag ccacaacggc tatgccagcc gctatggtct gacccatcgc cggatcctga
     1981 tcctgcgcga cgatggcagc gaactgcgcg gcgaagacct gctggtgcca gccgcccgca
     2041 agggcaagaa cggcaaggtc ggtttcgcga tccgctttca ccttggtccc ggggtcgagg
     2101 tgcggctttc ggaagacggg cagggcgcgg gcctggcctt gcccgacggc tcttactggc
     2161 agttccgtgc cgggggcggg cagctgggta tcgaagaaag catctgggcc gacgggcaag
     2221 gccgtccggt gccgatccac cagctggtga ttcacgggct ggtatcgcgc ggcggcggca
     2281 actttgcctg gctgctcaag aa
//
LOCUS       DASUDV010000173         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2126214,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000173 DASUDV010000000
VERSION     DASUDV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126214"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFV30_03630"
     CDS             complement(<1..1098)
                     /locus_tag="VFV30_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX4847210.1"
                     /translation="MEDIFIGLGANGERQQLLLGRANRHGLIAGATGTGKTVTLQTLA
                     EQFSAQGVPVFMADVKGDLAGISMPGSHEFKHADKLEARAAEIGIADYAYRDNPAVFW
                     DLYGEAGHPIRTTISEMGPLLLSRLMGLNDTQEGVLTIAFKWADDNGLLLIDFDDLQQ
                     VLIACAENASELATTYGNISRASVGTIQRQLLAFESQGADKFFGEPAFEINDFIRCDE
                     NGRGIINVLAADKLMQSPKLYATFLLYLLSELFEALPEVGDPEKPKLVFFFDEAHLLF
                     DDAPQALFDKIEQVVRLIRSKGVGVYFITQNPIDIPDKIAGQLGNRVQHALRAFTPRD
                     QKAIKAAAETFRINPDLDVETAITELKVGEAL"
     gene            1211..>2532
                     /gene="pabB"
                     /locus_tag="VFV30_03635"
     CDS             1211..>2532
                     /gene="pabB"
                     /locus_tag="VFV30_03635"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX4847211.1"
                     /translation="MTDPFILLDDARGDGASDARLYRAPEHVVVARRAEEVGPALERL
                     EALRGQGFELAGYLAYEAGLALEERLAPLANARSGTAGPLLWFGAFTGHEVIPAEQVP
                     AWLAGHGTGSPAQIGPLDPQLSIGGYARAFGALQEKIAAGDIYQSNLTFPLAGSFRGD
                     PLALYAALRPAAGAGYGGVVYDGTHWLLSCSPELFFAERDGAAMVKPMKGTRPRGGDP
                     AEDAAYASELAASEKDRAENLMIVDLLRNDLSRVAEAGSVKVERLFAVESYPTVHQMV
                     TTVRARLMQGMGAIDMVRALFPCGSITGAPKIRAMELIDAVERDARGPYCGAIGRIDT
                     AGDAAFNVAIRTLRLTPGENARGRAVLGVGSAIVADSEMMVEWRECLVKGGFVRMNGG
                     GGDLIETMRFTPDEGMPLLELHLERIKASAAQLGFAFDRHAVRNAIQAL"
BASE COUNT          407 a          793 c          869 g          463 t
ORIGIN      
        1 cagcgcctcg cccaccttga gttcggtgat cgcggtttcg acatcgagat cggggttgat
       61 ccgaaaggtt tcggctgccg ccttgatcgc cttctgatcg cgcggggtaa aggcgcgcag
      121 cgcgtgctgg acccggttgc caagttgccc tgcgatcttg tcggggatgt cgatcgggtt
      181 ctgggtgatg aaatagaccc ccacgccctt cgaacggatc agccgcacca cctgctcgat
      241 cttgtcgaac agcgcctggg gggcatcgtc gaacaagaga tgcgcctcgt cgaagaagaa
      301 caccagcttg ggcttttccg gatcgcccac ctcgggcagc gcctcgaaca gttcggagag
      361 gagatagagc aggaatgtgg cgtagagctt ggggctctgc atcagcttgt cggcggcgag
      421 cacgttgatg atcccgcgcc cgttctcgtc acagcgaatg aagtcgttga tctcgaaggc
      481 cggctcgccg aagaacttgt cggccccctg gctttcgaag gcgagcagtt ggcgctggat
      541 cgtgccgacg ctggccctcg atatgttgcc ataggtggtg gccagttcgc tcgcgttttc
      601 ggcgcaggcg atcaacacct gttgcagatc atcgaaatcg atcagcagga ggccgttgtc
      661 gtcggcccac ttgaaggcga tggtcagcac cccttcctgg gtatcgttaa gccccatcag
      721 ccgcgaaagc agcagcggcc ccatttccga aatggtcgtg cggatcgggt gcccggcctc
      781 gccatagagg tcccagaaaa ccgcgggatt gtcgcgatag gcatagtcgg caatgccgat
      841 ctcggcggcg cgcgcctcaa gcttgtcggc gtgcttgaat tcgtggctgc ccggcatcga
      901 gatcccggcc agatcgcctt tcacgtcggc catgaacacc ggcacgccct gggccgagaa
      961 ctgttcggcc agcgtctgca gggtcacggt cttgccggta cccgtggcgc ccgcgatcag
     1021 gccatggcga ttggcccggc ccagcagcag ttgctggcgt tcgccgttgg cgccaaggcc
     1081 gatgaaaatg tcttccatgt gcttcgcttt cttgaacgaa tctcgatcgg gccaagggat
     1141 aggccgcgcc gcagccagtt gcgagaggct ttttgcaagt agcgccgctc ggcgctagaa
     1201 gcgccgggca atgaccgatc cgtttatcct gcttgacgac gcccgtggcg atggggccag
     1261 cgacgcgcgg ctctatcgcg cgcccgaaca cgtggtggtg gcgcgccgcg ccgaagaggt
     1321 cggccccgcg ctcgaacggc ttgaggcgct gcgcggccag ggcttcgagc tcgctggcta
     1381 tcttgcctat gaagccgggc tggcactgga agagcggctg gccccgctgg ccaatgcccg
     1441 cagcggcacg gccgggccgt tgctgtggtt cggtgccttt accggccacg aggtgattcc
     1501 cgccgaacag gttccggcct ggctggccgg ccatggcacg ggcagccccg cgcagatcgg
     1561 cccgctcgat ccgcagcttt cgatcggcgg ctatgccagg gcttttggcg cgcttcagga
     1621 aaagatcgcg gcgggcgata tctatcagtc gaacctgacc ttcccgctgg ccggatcgtt
     1681 ccgcggtgat ccgctggcgc tttacgccgc gctgcgcccg gcggcaggtg caggttacgg
     1741 cggggtggtg tatgatggca cccactggct gctgtcgtgc tcgcccgaac tgttctttgc
     1801 cgagcgcgat ggcgcggcga tggtcaagcc gatgaagggc acccgcccgc gtggcggcga
     1861 tccggccgag gatgcggcct atgccagcga actggccgca tcggaaaagg accgggccga
     1921 aaacctgatg atcgttgacc tcttgcgcaa cgacctgtcg cgcgtggccg aggccggcag
     1981 cgtcaaggtc gagcggctgt tcgcggtcga aagctatccc accgtccacc agatggtgac
     2041 tacggtgcgc gcgcggctga tgcaggggat gggcgcgatc gacatggtcc gcgcgctgtt
     2101 cccttgcggc tcgattaccg gtgcgcccaa gatccgggcg atggagctga tcgacgcggt
     2161 cgaacgcgat gcccgcggcc cctattgcgg ggcgatcggc cggatcgaca cggccggcga
     2221 tgccgccttc aacgtggcga tccgcaccct gcgcctgacc cctggcgaaa acgcgcgcgg
     2281 ccgcgcggtg ctgggggtag gctcggcgat cgttgccgac agcgagatga tggtcgaatg
     2341 gcgcgaatgc ctggtcaagg gcgggttcgt gcggatgaac ggcggcggcg gcgacctgat
     2401 cgagacgatg cgcttcaccc ctgatgaggg catgccgctg ctcgaactgc atctcgagcg
     2461 gatcaaggcg agcgcggcgc agctgggctt tgccttcgat cgccacgcgg tgcgcaacgc
     2521 gatccaggcg ct
//
LOCUS       DASUDV010000174         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_668162,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000174 DASUDV010000000
VERSION     DASUDV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668162"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1084)
                     /locus_tag="VFV30_03640"
     CDS             complement(38..1084)
                     /locus_tag="VFV30_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX4847212.1"
                     /translation="MFKKSTLTLAFALSASALVAGCGGAQSGSREQIRAVGSSTVYPF
                     AKAVADSLAKSNSAFKSPIIESTGTGAGMKLFCGGVGVQHPDMVNASRRMKKSEFEDC
                     AKNGVDKIVEVQVGLDGIAFAEAKGGLALKLTQEDVYKALAANPYGKPNTAKTWKDVN
                     PSLPAEPILVYGPPSTSGTRDALKELVLMKGCDANAEMKALKDTDKAKHEAICTEVRN
                     DGAYVDAGENDNLIVQKLEANPKAVGVFGFSYLEENADKLVGLPMNGVMPTYAAISDF
                     SYPGARPLYIYVKAAHLEAIKGLKEFLVEWTKTWGKDGMLAKQGMVVAPDDVLAKSTK
                     AATEFTLLDPSTLK"
     gene            complement(1189..2367)
                     /locus_tag="VFV30_03645"
     CDS             complement(1189..2367)
                     /locus_tag="VFV30_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847213.1"
                     /translation="MEAKPALPLAGLTLALVTAGGMLLAGASWWLVVAVTVLWLGSLW
                     LSQPEPEERAERQDAKAVSRDAVHETIEPLGLPLLLLDGTRIVDANAAARSALGAHVL
                     GQDARIALRHPDAMRLGDMGDGESVTIPGFTGAKSLWQLTRRNLDSRRWIIELADRSS
                     EADVSKAHTDFVANASHELRTPLAAIGGYVETLADGGVDEATTQRFLGIVQRETRRMQ
                     SLVEDLMTLSHAEAEKHDRPSEELHLGRLAARVVGEVSSLRGKERVELTGSDESAMVL
                     GDAGQLEQVLRNLIDNALKYGDPDQPVRVSVARGDSRVRLSVADRGAGIAPEHLPHLT
                     RRFYRTDPGRSRASGGTGLGLAIVKHIVERHSGTLEIDSVVGEGTTVTVVLPMAEARG
                     "
     gene            complement(2488..2563)
                     /locus_tag="VFV30_03650"
     tRNA            complement(2488..2563)
                     /locus_tag="VFV30_03650"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2527..2529),aa:Glu,seq:ttc)
     gene            complement(2643..>4318)
                     /gene="recJ"
                     /locus_tag="VFV30_03655"
     CDS             complement(2643..>4318)
                     /gene="recJ"
                     /locus_tag="VFV30_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX4847214.1"
                     /translation="DIVAQLLLSRGVPAEDLDRHRNPSLRAFLPDPSVFRDMDVAAER
                     LADAVQNQEAVTVYGDYDVDGATSAALLIRLLRTLGLEARYYIPDRLLEGYGPSGEAL
                     VRLGQEGSSLVVTVDCGAMAHQALQMAREAGVEVIVVDHHKCSAELPPAVALVNPNRL
                     DENDTAAAHGHLAAVGVAFLLAVATVRVLRARGHFTGRAEPDLFALLDLVALGTVADV
                     AALHGLNRAFVAQGLKVMARRDGVGMAALIDASRLSRAPTCSDLGFALGPRINAGGRV
                     GESTLGVRLLTTEDPDEARAIAQQLSALNEERRAIEAAVQEAAEAQLESQHNRAVLTL
                     AGRGWHPGVIGIVAGRIKEKTGKPALVIALDADEAGNGKGSGRSISGVDLGAAIIAAR
                     EHGLLIAGGGHAMAAGLTVAPDKLEALGDWLDERLGSTVAAAMSGQSLLLDLAVAPGG
                     LTPGLVEELESAGPYGMGWPGPRVAVGPVRLVKADLVGTDHVRLIVRGEDGGNFKAIA
                     FRAASTEMGQTLLHATHHRRLWLAGRAKIDDWASRPAAELHVDDAAFAD"
BASE COUNT          641 a         1478 c         1487 g          712 t
ORIGIN      
        1 gcagtgctgg cgacatcggt gcgcttctag gccaagctta cttcagcgtc gaggggtcga
       61 gcagggtaaa ctcggtcgcc gccttggtgc tcttggccag gacatcgtcc ggagccacga
      121 ccatgccctg cttggccagc atgccgtcct tgccccacgt cttggtccat tcgaccagga
      181 attccttcag gcccttgatc gcttcaaggt gcgcggcctt gacgtagatg tagagcggac
      241 gggcgccggg gtagctgaag tccgagatcg ccgcgtaagt cggcatcacg ccgttcatcg
      301 gcagaccgac cagcttgtcg gcgttttctt ccagatagga gaagccgaac acgccgaccg
      361 ccttggggtt ggcttccagc ttctgcacga tcaggttgtc gttctcgccg gcgtccacat
      421 aagcgccgtc gttgcgcact tcggtgcaga tcgcttcgtg cttggccttg tcggtgtcct
      481 tcagcgcctt catttcggcg ttggcgtcgc agcccttcat cagcaccagt tccttgagcg
      541 cgtcacgcgt gcccgaggtg ctcggcgggc catagaccag gatcggttcg gccggcagcg
      601 aaggattgac gtccttccag gtcttggcgg tgttgggctt gccatagggg ttggcggcca
      661 gcgccttgta gacgtcttcc tgggtcagct tcagggccag accgcccttg gcttcggcaa
      721 aggcgatgcc atcgaggccg acctggactt cgacgatctt gtcgacgccg ttcttggcgc
      781 aatcttcgaa ctccgacttc ttcatgcggc gcgaagcgtt gaccatgtcg gggtgctgca
      841 cgcccacgcc gccgcagaac agcttcatgc cggcgccagt gccggtcgat tcgatgatcg
      901 gcgacttgaa cgccgagttc gacttggcga gcgagtccgc caccgccttg gcaaagggat
      961 agacggtcga ggagccgacc gcgcggatct gctcgcgcga gccggactgg gcgccgccgc
     1021 agccagccac gagggccgaa gcggaaagag cgaaggcaag ggtgagggtc gacttcttga
     1081 acatggcgaa tcgctccgtt cgattggata tgaccagcgc tctcgccgtg ttctgtgaca
     1141 gattaatgac aaggccttgg cgctttgccg aaggccggtg gccgcaggtc agccccgcgc
     1201 ttcggccatg ggcagcacaa ccgtgacggt ggtgccttcg ccgaccaccg aatcgatctc
     1261 cagcgtgccg ctgtggcgct cgacgatgtg cttgacgatg gcaaggccca ggccggtgcc
     1321 gcccgatgcc cggctgcggc ccggatcggt gcggtagaac cggcgggtca ggtgcggcag
     1381 atgctccgga gcgatccccg ccccgcgatc cgccacgctc agccggaccc ggctgtcgcc
     1441 ccgggcgacc gagacccgca ccggctggtc cggatcgccg tatttcaggg cgttgtcgat
     1501 caggttgcgc agcacctgct ccagctggcc ggcatcgccc aggaccatcg cgctctcatc
     1561 gctgccggtc agttcgacgc gctccttgcc gcgcagcgag gacacttcgc ccaccacccg
     1621 ggcggccagg cggcccaggt gcagttcttc gcttggccgg tcatgcttct ccgcctcggc
     1681 atggctcagc gtcatcaggt cttcgaccag gctttgcatc cggcgggttt cgcgctggac
     1741 gatccccagg aagcgctggg tcgtcgcttc gtccacaccg ccgtcggcca gggtttcgac
     1801 atagccgccg atcgcggcca gcggcgtgcg cagctcgtgg ctggcattgg cgacgaaatc
     1861 ggtgtgggcc ttgctgacat cggcttcgga gctgcggtcg gccagttcga tgatccagcg
     1921 ccggctgtcg aggttgcggc gggtcagctg ccacaggctt ttcgcgccgg tgaagccggg
     1981 gatggtcacg ctttcgccgt cacccatgtc gcccagccgc atcgcatcgg gatggcgcag
     2041 ggcgatccgc gcgtcctggc ccaggacgtg ggcgcccagc gcagagcggg ccgcggcgtt
     2101 ggcatcgacg attcgggtcc cgtcgagcag cagcagcggc aggcccagcg gctcgatcgt
     2161 ttcgtgaacg gcatcgcgcg acacggcctt ggcgtcctgc cgttcggcgc gctcttcggg
     2221 ttcgggctgg gacagccaca gcgaaccgag ccagagcacc gttaccgcaa caaccagcca
     2281 ccagctggcc ccggccagca gcatcccgcc ggcggtcacc agcgccaggg tcagtccggc
     2341 aaggggaagg gcaggtttgg cttccatcgg cgtgcgctca ataaagggcg aatggcactg
     2401 tgccaaatgg ttccggtgct tgggggagag tggccgttcc cggcgaatcg ccgggcgcag
     2461 acagcacaat gcccaccgtg cgctggttgg tgacccctac gggactcgaa cccgtgtttc
     2521 agccgtgaaa gggccgcgtc ctagaccgct agacgaaggg gccacaccgg ctgcgcggtg
     2581 ggcaggcccg cgcctttagg caaggagctt gagagggtca aggggcaaag ggcaagaata
     2641 tttcaatcgg cgaaggcggc gtcgtcgacg tgcagttccg ccgccggccg gctggcccaa
     2701 tcgtcgatct tcgcccggcc tgccagccac agccgccggt ggtgcgtggc gtggagcagc
     2761 gtctggccca tctcggtcga ggcggcgcga aaggcgatgg ccttgaagtt gccgccgtcc
     2821 tcgccgcgca cgatcaggcg gacatggtcg gtgccgacca gatcggcctt gaccagccgc
     2881 accggaccga ccgcgacccg cgggcccggc cagcccatgc cgtagggtcc ggcgctttcc
     2941 agctcctcga ccaggcccgg ggtcagcccg ccgggggcga ccgccaggtc cagcagcagc
     3001 gactgcccgc tcatcgcggc ggccacggtg ctgcccagcc gttcgtccag ccagtcgccc
     3061 agcgcctcca gcttgtccgg ggccaccgtc agcccggccg ccatggcgtg tccgccgccc
     3121 gcgatcagca ggccgtgctc gcgcgcggcg atgatcgcgg cgcccaggtc cacgccggaa
     3181 atcgagcggc ctgaaccctt gccgttgccg gcctcgtcgg cgtcgagggc aatcaccagc
     3241 gcgggctttc cggtcttctc cttgattcgc ccggcaacga tgccgatcac cccggggtgc
     3301 cagccgcgcc cggccagggt cagcaccgcg cggttgtgct gcgattcgag ctgggcctcg
     3361 gcggcttcct gcaccgcggc ctcgatcgcg cggcgttcct cgttcagggc agacagctgc
     3421 tgcgcgatgg cgcgggcctc gtcggggtcc tcggtggtca gcagccgcac gcccagggtc
     3481 gattcgccca cccgcccgcc ggcgttgatc cgcgggccaa gggcaaagcc cagatcgctg
     3541 caggtcgggg cgcggctcag gcggctggcg tcgatcaggg cggccatgcc aaccccgtcg
     3601 cgccgggcca tcactttcag cccctgggcc acgaaggccc ggttgagccc gtgcagtgcg
     3661 gcgacatcgg cgacggtccc cagcgccacc agatcgagca gcgcgaagag atcgggctcg
     3721 gcccggccgg tgaagtggcc gcgggcgcgc aggacgcgga ccgtggcgac cgccagcaga
     3781 aaggccacgc caacggcggc cagatggccg tgcgcggcgg cggtgtcgtt ctcgtccagc
     3841 cggttgggat tgaccagcgc gacggccggg ggcagctcgg cagagcactt gtggtggtcg
     3901 accacgatca cttcgacccc ggcctcgcgc gccatctgca gcgcctggtg ggccatggct
     3961 ccgcaatcga cggtcacgac caggctggag ccttcctgcc ccagccggac cagcgcctcg
     4021 cccgaggggc catagccttc gagcagccgg tcggggatgt aatagcgcgc ctccagcccc
     4081 agcgtgcgca gcaggcggat cagcagcgcg gcgctggtcg ccccgtcgac gtcgtaatcg
     4141 ccatagacgg tgaccgcttc ctggttctgc accgcatcgg ccagccgctc ggccgcgacg
     4201 tccatgtcgc ggaacaccga agggtcgggc aggaaggcgc ggagcgaggg gttgcggtgg
     4261 cggtcgaggt cttcggccgg caccccgcgc gaaagcagca gctgcgcgac gatatcgt
//
LOCUS       DASUDV010000175         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1971838,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000175 DASUDV010000000
VERSION     DASUDV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971838"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFV30_03660"
     CDS             complement(<1..609)
                     /locus_tag="VFV30_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847215.1"
                     /translation="MNSINARAPISAARPMPSIGAVPKSFLLLIMAWLFDFPAESAGE
                     GLSTQQYFAGIYIVSTLLFVIGDRDDGRRVPGLMLTMVLGALYLVIGIAVGLAENQPT
                     YSVLRNGLTVFVYLTAIYTTARVALMSDPARLRWFLGIACLLYALANLLIDYLGAGGI
                     DLASVRYQIVGVSVNAALAYITLALLFRLSRTEWITMLANGVI"
     gene            complement(718..1947)
                     /locus_tag="VFV30_03665"
     CDS             complement(718..1947)
                     /locus_tag="VFV30_03665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4847216.1"
                     /translation="MKPASRLPQIALIWSQFTDTHVDRCDAVADRFSGRAEILAVEVA
                     TTSTLYAQFSPGREMGAARKVTLFPGQSFDAISRWRRLWKCLLAVRKARVVCLGVSYG
                     EWDFLLLCWLLRLAGKRVILTSCSKFDDFPRRAWFELVKAAALSCFEGVLTSGGRGRD
                     YFRFLGFRRRPIVTGYNSLALDRIRAQVASAVRGAAPDFVRRDFVFVGRFIDKKDLSV
                     LIEAFARYLELEPASQRRLVLIGGGPMERELREQAARQVPDGRVEFPGFLEGAELYGR
                     VAQGLCLVLVSCSEQWGLVINEAMALGQPIIASRAPGARDILVRNLINGFVLELGSVE
                     GIARAMQRMGADEAAWAAMSSASGERAAMGDARVFAQALEQLVERHSGQPAADHPHKQ
                     ALGELGDADEKVRKELA"
     gene            complement(2010..>2705)
                     /locus_tag="VFV30_03670"
     CDS             complement(2010..>2705)
                     /locus_tag="VFV30_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4847217.1"
                     /translation="GYDTLSTERVRRLAGLDADFAAPAFATRDFVYVGRFIPKKGLTS
                     LISAFARFCEIEPESKRKLIMIGTGPLDRELREQVSALGLGERVEFTGFIEGSALYRR
                     MAQALALVFVSYREQWGLVLNEATALGLPLIVSEAPGAGDILARNLVNGFVLENASIE
                     GIARAMQDISNDEDQWHRMSAASAELASHGDCALFADAIELTIDERAQPARQRIAGYV
                     ARFPTLQHRPLVD"
BASE COUNT          530 a          900 c          842 g          433 t
ORIGIN      
        1 gatgacgccg ttggcgagca tcgtgatcca ttcggtccgg cttagccgga acagcagcgc
       61 cagggtgatg taggccagcg cggcattcac cgagaccccg acgatctgat agcgcacgct
      121 cgccagatcg atgccgcccg cccccaggta gtcgatcaga agattggcca gggcatagag
      181 caggcaggcg atgcccagaa accagcgcag ccgcgccgga tcgctcatca gtgcaactcg
      241 cgcggtcgta tagatcgcag tgaggtagac gaacaccgtc aagccgttgc gcagcaccga
      301 ataggtcggc tggttttcgg ccagcccgac cgcgatgccg atcaccagat agagcgcgcc
      361 cagcaccatg gtcagcatca gcccgggcac tcgccggcca tcatcgcgat caccgatcac
      421 gaacagcagt gtggaaacga tgtagatacc ggcaaaatac tgctgggtgc tgagcccttc
      481 gccggcggat tcagcaggaa agtcgaacag ccaggccatg atcagcagca ggaacgattt
      541 cggcaccgcg ccgatcgatg gcatcggccg cgcggcggaa atcggcgctc tggcgttgat
      601 ggaattcatg gcgctatctg cctgcctgtc gaatgtgccg catccagggc ctgccatggc
      661 atgtccggcg caacacggca aatgccgctg gcttggcgcc gatgggctgg cgatcgatca
      721 tgccagttcc tttcggacct tttcgtcggc atcgcccagt tcgccaagcg cctgcttgtg
      781 cggatgatcg gcagccggct gtcctgaatg ccgttcgacc agctgttcga gtgcctgcgc
      841 aaagactctg gcatcgccca tcgccgcgcg ctcgcccgaa gcgctgctca tcgcggccca
      901 ggccgcttca tccgcgccca tgcgctgcat cgcccgtgcg atcccttcaa ccgagcccag
      961 ttcgagcacg aaaccgttga tcaggttgcg caccaggatg tcgcgcgcgc cgggtgcgcg
     1021 cgatgcgatg atcggctggc cgagcgccat ggcttcgttg atcaccagcc cccattgttc
     1081 gctgcagctt accagcacca ggcacaggcc ctgggcgacc cggccataca attctgcgcc
     1141 ttcgagaaag ccggggaatt cgacacggcc atccggcacc tgccgcgcgg cctgttcgcg
     1201 cagttcgcgc tccatcggtc cgcccccgat cagcaccagc cgccgctggc tggccggttc
     1261 aagctcgagg tagcgggcaa aggcctcgat cagaaccgac aggtccttct tgtcgatgaa
     1321 ccgtccgaca aagacaaaat cgcggcggac gaagtcaggc gccgcgcccc ggacggcact
     1381 ggcaacctgg gcgcggatcc ggtcgagagc gagcgaattg tagcccgtga caatcgggcg
     1441 cctgcgaaac ccaaggaagc ggaaatagtc gcgcccgcgc ccgcctgagg tcagcacgcc
     1501 ctcgaagcac gacagcgcag cggccttgac cagttcgaac caggcccggc gcggaaaatc
     1561 atcgaacttc gaacaggaag tgagaatcac ccgtttaccg gcaagccgaa gcagccagca
     1621 cagcagcagg aaatcccatt cgccatagga aaccccgaga cagacgacgc gcgccttgcg
     1681 caccgcaagc aggcacttcc agaggcggcg ccagcgggaa atcgcgtcga acgactggcc
     1741 gggaaacagc gtcaccttgc gcgccgcccc catttcgcgg cccggactga actgggcata
     1801 gagcgtcgag gttgtggcaa cctcgaccgc caggatttcg gcccggccgc tgaaccgatc
     1861 ggccaccgcg tcgcagcgat cgacatgggt gtcggtgaac tgcgaccaga tcagcgcgat
     1921 ctgcggcagg cgggacgcag gcttcaagga cggccttggc gaaacttcct gtgctgcgag
     1981 cgaagttgat ctgttcgtca tgcccgcgct caatccacca gggggcgatg ctgcagcgtc
     2041 gggaagcggg cgacatagcc ggcgatccgc tgccgcgcag gctgcgcccg ttcatcgatc
     2101 gtcagttcga tcgcatcggc gaacagggcg caatcgccgt ggcttgccag ttcggccgat
     2161 gccgcgctca ttctgtgcca ctgatcttcg tcgttcgaaa tgtcctgcat ggcgcgcgcg
     2221 attccctcga tcgaggcatt ttccaggacg aaaccgttca ccaggttgcg ggccaggatg
     2281 tccccagccc ccggtgcctc ggacacgatc agcggcaggc caagcgcggt cgcctcgttc
     2341 aggaccaggc cccattgctc gcggtagctg acgaacacca gggcgagcgc ctgggccatc
     2401 cggcgataga gtgcggagcc ttcgatgaag ccggtgaatt caacccgctc accaagaccg
     2461 agtgcgctga cctgctcgcg cagttcccgg tcaagcggcc cggtgccgat catgatcagc
     2521 ttgcgcttgc tttcaggctc gatttcgcag aaccgcgcga aggccgaaat cagcgatgtc
     2581 agccccttct tggggatgaa acgcccgaca tagacgaaat cgcgcgtggc gaaggcaggc
     2641 gccgcgaaat cggcatcgag cccggccagc cggcgcactc gctcggtgct caaggtgtca
     2701 tagcc
//
LOCUS       DASUDV010000176         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2148379,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000176 DASUDV010000000
VERSION     DASUDV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148379"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..539)
                     /gene="ilvN"
                     /locus_tag="VFV30_03675"
     CDS             complement(24..539)
                     /gene="ilvN"
                     /locus_tag="VFV30_03675"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX4847218.1"
                     /translation="MKIKHEASERHVLTITVDNEAGILAKIAGLFTARGYNIDSLTVA
                     DITENHAVSRITIVTHGPPAVIDQIHAQLDRLVPVHKVIDLTEAGDHVERELALVKVS
                     GTGDARVEALRLAEVFRAKVVDTTTSSFIFELTGTPDKLDRFVSLMRELGLVEVGRTG
                     VVGMMRGAEAA"
     gene            complement(545..2287)
                     /gene="ilvB"
                     /locus_tag="VFV30_03680"
     CDS             complement(545..2287)
                     /gene="ilvB"
                     /locus_tag="VFV30_03680"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX4847219.1"
                     /translation="MSEERSGASILVESLVRQGVEFVFGYPGGAVLPIYDALFGDERI
                     RHVLVRHEAGAAHAAEGYARSTGKAGVVLVTSGPGATNAVTGIADAFMDSIPMVVITG
                     QVATGLIGSDAFQEADTIGITRHCTKHNYLVKDPADLAAVIDEAFQIATTGRPGPVVI
                     DIPKDVQVASAPFSEGPPQRKIRYSPPSTGSERDIARAIELLANAKAPVLYTGGGVIN
                     SGPRAAELLRELQALTGAPLTSTLMGLGAFPADHPDWLGMLGMHGTYEANMAMNRADL
                     MVCIGARFDDRVTGRLDAFAPNSTKIHIDIDRASINKTVPVDLPILGDCAQVLEQLVV
                     AWGDRKAQDLTEWKARIEGWRARKSLAYPARADAIMPQLAIERLFALTRDRDPVIATE
                     VGQHQMWAAQYFHFFGPNKWLTSGGLGTMGYGLPAAIGAQLGNPDSLVIDIAGDASIQ
                     MNIQELGTATQYRLPVKVFVLNNEWMGMVRQWQELTYESRYSNSYSDSLPDFVKLAEA
                     YGWKGIRIEDESQLDAGIQAMIDHPGPVIVDCLVAKEANCYPMIPSGAAHTDMILYGD
                     QVAGTMDDEAKALV"
     gene            complement(2451..3419)
                     /gene="miaA"
                     /locus_tag="VFV30_03685"
     CDS             complement(2451..3419)
                     /gene="miaA"
                     /locus_tag="VFV30_03685"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX4847220.1"
                     /translation="MSTLESPESVAARSGKPPLALIAGPTASGKSDVAVQLALALAKQ
                     GREGVVINADSAQVYADLAVLSARPAPAEMHGVEHRLFGEWDGAEACSAADWAARARD
                     VIAEVHARGGVPILVGGTGLYLRTLLDGIAPVPPIDPAVREAVRALPVSEAYAALLAE
                     DPERAARLAPGDSTRVARALEVVRSTGRPLADWQADRVGGIGDAVTLHARVLLPPRDW
                     LYERCDRRFAQMLEGGALDEVARLVARQLDPDLPVMRAIGVPALAALLAGTLSREEAA
                     ARGAQATRHYAKRQYTWFRNQPPPEWQRIESFDYDTNAIFASLLRY"
     gene            3412..4287
                     /gene="serB"
                     /locus_tag="VFV30_03690"
     CDS             3412..4287
                     /gene="serB"
                     /locus_tag="VFV30_03690"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX4847221.1"
                     /translation="MLIARVIAEPAVLSAHVDAAVHALESQGVRVLGADPLAGLEEVL
                     QIMADQGDPAAVRGVLDRHFSPSDMLITDHLPQTPGLFVSDMDSTMIGQECIDELADF
                     AGIKDRIAEITERAMQGELDFAEALAERVRLLGGLGEDAIQQCLDERIRDMPGAATLV
                     ATLRAHGCATVLVTGGFHQFADPVAERLGFEQVVGNRLAVAHGKLTGALVGAIVDSSV
                     KEAVLRREAERRGGAVSLATGDGANDIPMLVAAHYGVAYHAKPKARAAANGAIDRGDL
                     TDVLRLLGIPESDWA"
     gene            complement(4284..>4501)
                     /locus_tag="VFV30_03695"
     CDS             complement(4284..>4501)
                     /locus_tag="VFV30_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847222.1"
                     /translation="LTSNDAAMRALGRNWKRLHTFGIHYVWLIFTLAYAGRLMEAETR
                     PEALYGVSLCLAALAVRIAAWRQGRRA"
BASE COUNT          708 a         1497 c         1556 g          740 t
ORIGIN      
        1 ggtttccgtg attgaaattc cgatcaggcc gcttcggccc cgcgcatcat gccgaccacg
       61 ccggtgcggc caacctcgac caggcccagt tcgcgcatca gcgagacaaa gcggtcgagc
      121 ttgtccgggg tgccggtcag ctcgaagatg aagctgctgg tggtggtgtc gaccaccttg
      181 gcgcggaaca cttcggccag gcgcagggcc tcgacccggg catcaccggt gccggagacc
      241 ttgaccagcg ccagctcgcg ctcaacgtgg tcgccggctt cggtcaggtc gatcaccttg
      301 tggaccggga ccagccggtc cagctgggca tggatctggt cgatcaccgc gggcgggccg
      361 tgggtgacga tggtgatccg gctgaccgcg tggttttcgg tgatgtcggc cacggtcagg
      421 ctgtcgatgt tgtagccgcg cgcggtgaac aggccggcga tcttggccag gatcccggcc
      481 tcgttgtcga ccgtgatggt cagaacgtgg cgttccgagg cttcgtgctt gatcttcatc
      541 atgattacac cagagccttc gcttcgtcgt ccatcgtgcc ggccacctga tcgccataaa
      601 ggatcatgtc ggtgtgcgcc gcgcccgaag ggatcatcgg atagcagttc gcttccttgg
      661 cgacgaggca atcgacgatc accggacccg gatggtcgat catcgcctgg atcccggcgt
      721 cgagctggct ttcgtcctcg atccggatgc ccttccagcc ataggcctcg gccagcttca
      781 cgaaatcggg cagactgtcc gaataggagt tcgaatagcg gctttcatag gtcagttcct
      841 gccactggcg gaccatgccc atccattcgt tgttgagcac gaagaccttc accggcaggc
      901 ggtactgcgt ggcggtgccc agttcctgaa tgttcatctg gatcgaggcg tcgccggcaa
      961 tgtcgatcac caggctgtcc ggattgccca gctgcgcgcc gatcgcggct ggcaggccat
     1021 agcccatggt gcccagaccg cccgaggtca gccacttgtt gggcccaaag aagtggaagt
     1081 actgcgccgc ccacatctgg tgctggccca cttcggtggc gatcaccgga tcgcgatcgc
     1141 gggtcagcgc gaacagccgt tcgattgcca gctgcggcat gatcgcatcg gcgcgggccg
     1201 gataggccag gctcttgcgg gcgcgccagc cttcgatccg ggccttccac tcggtcagat
     1261 cctgggcctt gcggtcgccc caggcgacaa cgagctgctc cagcacctgg gcgcaatcgc
     1321 ccaggatcgg caggtcgacc ggcacggtct tgttgatcga ggcgcggtcg atatcgatgt
     1381 ggatcttggt cgaattgggg gcaaaggcat cgagccgccc ggtcacccgg tcgtcaaagc
     1441 gcgcgccgat gcaaaccatc aggtcggccc ggttcatcgc catgttggct tcataggtgc
     1501 cgtgcatgcc cagcatgccc agccagtccg gatggtcggc cgggaaagcg cccaggccca
     1561 tcagggtgga ggtgaggggg gcgccggtca gcgcctgcag ctcgcgcagc agttcggccg
     1621 cgcgcgggcc ggaattgatc acgccgccgc cggtgtagag caccggggcc ttggcattgg
     1681 cgagcagttc gatcgcccgg gcaatgtcgc gctcgctgcc ggtggaggga gggctgtagc
     1741 ggatcttgcg ctgcggcggg ccctcgctga acggggccga ggcaacctgg acgtccttgg
     1801 gaatgtcaat cacgaccggg ccggggcggc cggtggtggc gatctggaac gcctcgtcga
     1861 tcaccgcggc gaggtccgcc gggtccttca ccagatagtt gtgcttggtg cagtggcggg
     1921 tgatgccgat cgtgtcggct tcctggaacg cgtcggaccc gatcaggccg gtggcgacct
     1981 ggccggtgat gacgaccatc gggatcgaat ccatgaaggc atcggcaatg ccggtgacgg
     2041 cattggtcgc gcccgggccc gaggtgacca gcaccacgcc ggccttgccg gtggagcggg
     2101 catagccctc ggccgcgtgg gcggccccgg cctcgtgccg gaccagcacg tggcggatcc
     2161 gttcatcgcc gaacagggca tcgtagatcg gcagaaccgc gccgccggga tagccgaaga
     2221 cgaattcgac cccctggcga accaggcttt cgaccaggat gctggcgccg ctgcgctcct
     2281 cgctcacgtt gctttacctt tcacttcgct gccgcttttc cgcgccctgg cccctgaaag
     2341 gaccgacccg gcacaggggg ctgtgccggg tctcctctcc aactcgaagc caatgcgcaa
     2401 gcgcataacg ttgcgggcgc cctaggcgat ggaaaatatc gcgtcaaccc ctaatagcgc
     2461 aacaatgatg cgaatatggc attcgtgtcg taatcaaatg actcgatccg ctgccattcc
     2521 ggcgggggct ggttgcggaa ccaggtgtac tggcgcttgg catagtggcg ggtggcctgc
     2581 gcaccgcgcg cggcggcctc ttcgcggctg agggtgccgg ccagcagcgc cgccagtgcg
     2641 ggcacgccga tcgcgcgcat caccggcaga tcgggatcga gctgccgggc caccagccgc
     2701 gcaacctcgt ccagcgcgcc gccttccagc atctgcgcaa agcggcggtc gcagcgctcg
     2761 taaagccagt cgcgcggggg cagcagcacc cgggcgtgca gcgtcaccgc atccccgatc
     2821 ccgccaaccc gatcggcctg ccaatcggcc aaaggccgtc cggttgagcg caccacttcg
     2881 agcgcgcggg ccacccgggt gctgtcgcca ggggccagac gcgcggcccg ctccggatcc
     2941 tcggccagca gcgcggcata ggcttcgctg accggcaggg cccgcaccgc ttcgcgcacg
     3001 gcgggatcga tcggcgggac gggggcaatc ccgtcgagca gggtccgcag gtagagcccg
     3061 gtcccgccaa ccaggattgg cacgccgccg cgggcgtgga cttcggcgat cacatcgcgc
     3121 gcccgggcgg cccagtccgc cgcggaacag gcttcggctc cgtcccattc cccgaacagg
     3181 cggtgctcca ccccgtgcat ctcggccggg gcggggcggg ctgagaggac ggccaggtcg
     3241 gcatagacct gcgcgctgtc ggcgttgatc accacccctt cgcggccctg cttggcaagg
     3301 gccagggcga gctgcaccgc gacatcgctc ttgccgctgg cggtcggccc tgcgatgagc
     3361 gccagcggcg gcttgccaga tcgcgccgcg accgattcag gggattccaa cgtgctcatt
     3421 gcccgcgtga tagcagaacc ggcggtcttg tccgcccatg ttgatgcggc ggtccacgcg
     3481 ctggaatcgc agggcgtccg ggtgctgggt gccgatccgc tcgcgggcct cgaagaggtg
     3541 ctgcagatca tggccgatca aggcgatccg gccgcggtgc gcggcgtgct cgatcgccat
     3601 ttcagcccgt cggacatgct gatcaccgat cacttgccgc agacgccggg cctgttcgtc
     3661 tccgacatgg attcgaccat gatcgggcag gaatgcatcg acgagctggc cgactttgcc
     3721 gggatcaagg accggatcgc cgaaatcacc gagcgggcga tgcagggcga gctcgacttt
     3781 gccgaagcgc tggcggagcg ggtgcgcctg ctgggcgggc tgggtgaaga cgcgatccag
     3841 caatgcctgg atgaacgcat ccgcgacatg ccgggcgcgg ccaccctggt cgccaccctg
     3901 cgcgcccatg gctgcgccac ggtgctggtc accgggggct ttcaccagtt tgccgatccg
     3961 gtggccgaac ggctggggtt cgaacaggtg gtcggcaaca ggctggcagt ggcccacggc
     4021 aagctgaccg gcgcgctggt gggcgctatc gtcgacagtt cggtgaagga agcggtcctg
     4081 cgccgcgagg cggagcgccg gggcggggca gtcagcctgg cgaccggcga tggggccaac
     4141 gacattccga tgctggtcgc cgcccattac ggcgtggcct atcacgccaa gcccaaggcc
     4201 cgcgccgccg ccaacggcgc gatcgaccgg ggcgacctga ccgatgtgct gcggctgctg
     4261 gggattcccg aaagcgactg ggcctaggcg cgccgcccct gccgccaggc cgcgatccgc
     4321 accgccagcg cggccaggca cagcgacacg ccatagagag cttcgggccg cgtctcggcc
     4381 tccatcagcc gcccggcata ggccagggtg aagatcagcc agacatagtg gatgccgaag
     4441 gtgtgcagcc gcttccagtt gcgccccagc gcccgcatcg ccgcgtcgtt tgaggtcagc
     4501 g
//
LOCUS       DASUDV010000177         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_712658,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000177 DASUDV010000000
VERSION     DASUDV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712658"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFV30_03700"
     CDS             complement(<1..437)
                     /locus_tag="VFV30_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847223.1"
                     /translation="MKPPHPPRLIAPAHRRAIAGLIALMTLAALTEGLGIVLLVPLLG
                     VLGEGDAGAMGAALRAAGVPLRLDLLLALFVGLVTLRAVVVHLRTLAGLRFETAVVDA
                     LRHRAWAALLHCEWRTLLGMSRSNTASLLINRVDRAGTYVNQG"
     gene            complement(448..>2271)
                     /locus_tag="VFV30_03705"
     CDS             complement(448..>2271)
                     /locus_tag="VFV30_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX4847224.1"
                     /translation="PPGHFAQAQRFGRAVLAWADPARGREVGAGSEGWQVLFAGQLHN
                     RKALMGELGLSDPAPEAVYAAALDRWGTAADDHCIGHYAAIAIAPDGASMRLARSPFL
                     APPLHFRQADGAIVAAQRPRELFWNAANRPEPDLERVGQVLVNDASDRFRSWYRSAHR
                     LPLGSAVVVRSSGWDEIWRYDLFTRPQQRMARRSDYAEAAQALLDDGVGAVLEGVRKP
                     AIMLSGGLDSPLVAASILRQLGPDQRLEAFTFGPSSEWAANAPPGIFCNDFEPVAAFA
                     QMHPRLSVHFETNPGRDFRFGQRELLEASDAAPSSLGLAWIEHSSYAAAGALGCDAML
                     CGTWGNFGFSSRGPWAYSEFLRRGKWDQLYRALKARVGDQRPLWRQFAGLALAPLLPR
                     PIWQFGKRLVSQATDRLLPVAIRPDWPELEATLQRSRAAGFDFERLQFSSRTAHWRAL
                     LAEDGQEQDQYALGMELMHGLPKRDPTAYRPLIEFCWGCPTDVFIHQGTDRWLAREMA
                     KGQMPEDQRLNRAYGHHFVDWQPRLAAIRDELLDELERMADDPDIASVVDIPRLQQLV
                     AAIPERADGYDPQTALPYQTALPIGMAAARFIAYAKGRNDI"
BASE COUNT          406 a          762 c          746 g          357 t
ORIGIN      
        1 atgccctggt tgacataggt tccggcccgg tcgacccggt tgatcagcag gctggcggtg
       61 ttggaacggc tcattcccag cagggtgcgc cattcgcagt gcagcagggc ggcccaggcg
      121 cggtggcgca gcgcgtcgac caccgccgtt tcaaaccgca gcccggccag cgtccgcaag
      181 tggaccacga ccgcccgcag cgtcaccagg cccacgaaca gcgccagcag cagatcgagc
      241 cgcagcggca ccccggcggc gcgcagggcc gcgcccatcg cccccgcatc gccttcgccc
      301 agcacgccga gcagcggcac gagcagcacg atccccagcc cctcggtcag cgccgccagg
      361 gtcatcagcg cgatcagccc ggcgatggcg cggcggtggg ccggcgcgat caggcgcggc
      421 gggtggggcg gtttcacagt gggcgggtca gatgtcattg cggcccttgg cataggcaat
      481 gaaccgggct gcggccatcc cgatcggcag cgctgtctgg tagggaagcg ccgtttgcgg
      541 gtcatagccg tccgcgcgct cggggattgc ggcaacgagc tgctgcagcc gcggaatgtc
      601 gacaaccgag gcaatgtcgg gatcgtcggc catccgttcc agttcgtcga gcaattcgtc
      661 ccggatggca gccaggcgcg gctgccaatc gacgaaatga tggccatagg cccggttcag
      721 gcgctggtcc tcgggcatct gccctttggc catctcgcgg gctagccacc gatcggtgcc
      781 ctggtggatg aacacgtcgg tcggacagcc ccagcaaaac tcgatcagcg gccgataagc
      841 ggtcggatcg cgcttgggca ggccgtgcat cagttccatg cccagtgcgt attgatcttg
      901 ctcctggcca tcttctgcca gcagcgcccg ccaatgagcc gtgcggctgg agaattgcag
      961 ccgctcgaaa tcgaatcccg ctgcgcggga ccgctgcagt gtcgcctcaa gctcgggcca
     1021 atcaggccgg atcgccaccg gcagcaaccg gtcggtcgcc tgactgacaa gccttttgcc
     1081 gaattgccag atcggacgag gcaaaagcgg cgcgagggcg agcccagcaa attggcgcca
     1141 cagcggccgc tgatcgccaa cccgcgcctt caatgcccta tagagctgat cccattttcc
     1201 gcgtcgcaag aactcgctgt aagcccatgg accgcggctg ctgaatccga aattgcccca
     1261 ggtgccgcac agcattgcat cgcagcccag cgcgccggct gcagcgtagg agctgtgttc
     1321 gatccaggcg agtcctaaac tcgagggcgc ggcgtcgctc gcctccagca gttcccgctg
     1381 gccaaaccgg aaatcgcgtc ccggatttgt ttcgaaatgc accgacaaac gcggatgcat
     1441 ttgggcgaag gccgctaccg gctcaaaatc gttgcagaag atgccaggcg gcgcgtttgc
     1501 ggcccattcg cttgaggggc caaacgtaaa ggcctccagc cgctgatccg ggcccagttg
     1561 tcgcaggatg ctggcggcga cgaggggcga atcaagccca ccggacagca tgatcgccgg
     1621 tttgcgcacg ccttcgagca ccgcgccgac gccgtcgtca agcagcgctt gtgccgcttc
     1681 ggcgtagtct gaccgccgtg ccattcgttg ctgcgggcgg gtgaacagat cgtagcgcca
     1741 gatttcgtcc caaccgctgg atcggacgac cacagccgag cccagtggaa gacggtgggc
     1801 agaccggtac caggaccgga atctgtcact ggcgtcattg accagcacct gccccacccg
     1861 ttccagatcg ggttcaggcc ggttggccgc gttccagaac agttcacggg gccgctgagc
     1921 ggcgacgata gcaccgtcgg cctgccgaaa gtggagcgga ggggccagga aggggcttcg
     1981 tgccagccgc attgaagcgc catccggggc aattgctatg gcggcatagt ggccgatgca
     2041 atggtcgtcg gcagcggtgc cccaacggtc cagtgccgca gcatagaccg cttctggggc
     2101 cgggtcgcta agcccaagtt cccccatcaa ggccttgcgg ttgtgaagtt ggccagcgaa
     2161 gaggacctgc caaccttcgc taccggcccc aacctcgcga ccacgcgccg ggtcagccca
     2221 ggccagcacc gcccgtccaa agcgctgtgc ttgcgcgaaa tgtccgggcg g
//
LOCUS       DASUDV010000178         7723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2148438,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000178 DASUDV010000000
VERSION     DASUDV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148438"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..512
                     /locus_tag="VFV30_03710"
     CDS             39..512
                     /locus_tag="VFV30_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX4847225.1"
                     /translation="MMDEPKFTIEEVDSVAPPPRATVEAAETPAEKLERKRDYLDGFL
                     AQAPQGVAPGAPGGDLYEGVIAALKDIFDPEIPVNIYDLGLIYGVDVADDGGVVVTMT
                     LTTPHCPVAESMPGEVELRVGAVPGVRDAEVNLVWDPPWDPAKMSDEARLELGML"
     gene            512..868
                     /locus_tag="VFV30_03715"
     CDS             512..868
                     /locus_tag="VFV30_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX4847226.1"
                     /translation="MTETKIRQRPAAVILTPAAEARVAKLMADAPADAIGVKLSTPRR
                     GCSGLAYSVDYVTEASPLDEKIETPGGLFFIDGASVLYLIGSTMDWVEDDFTAGFVFQ
                     NPNAKGSCGCGESFTV"
     gene            complement(1096..2070)
                     /locus_tag="VFV30_03720"
     CDS             complement(1096..2070)
                     /locus_tag="VFV30_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847227.1"
                     /translation="MSLEFGNLTTQVAGDGHISAEEILELRRLGWADGKMSPDEAESL
                     FVANDTCDEPSAEWCDFFVEALTSFVVHTVEPRGYVDQEMADELVSRIDRDGHVGTLA
                     ELELLVKVIETATSVPASLRAYALRQIEDAVVGGEGPTRHGSLTREGINAAETLLIKR
                     LIFGTGSERPAGVSKAEAEMLFRIKDATLYEANAPEWQDLFVKGVAQFLLGFGGDEAL
                     SQERAAELEDFMASEGAGIGGFLHRMMTSKPDVDGFGSLLKIGGDEPDYLADWDDEAE
                     AASQLTPEEHSWLHDMLEADEELDEMEKALIAFIDEETGETFVPRPQG"
     gene            complement(2173..2586)
                     /locus_tag="VFV30_03725"
     CDS             complement(2173..2586)
                     /locus_tag="VFV30_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847228.1"
                     /translation="MTFRPVPVLALGLLLSACASGGTTTARPAPQTAGRVPVRVPVQA
                     PPPRPRVLTAPGLEGVIGATNADLVRQFGPARLEVWEGDARKLQFTGTACVLDVYLYP
                     PAPGREPEATYVEARRASDGQDVDRGSCVNALRGR"
     gene            complement(2583..3266)
                     /locus_tag="VFV30_03730"
     CDS             complement(2583..3266)
                     /locus_tag="VFV30_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX4847229.1"
                     /translation="MLQALALALPRMADRHLLPIWLKSLAVTLLVFAALGSAGWYGLA
                     GVLDRYARSYGMSHGYSGVLALLIVLIAGGLLWRILALAVLQFFADEVVHAIEARDYP
                     DMAARARKLGLAEEARRALAGAARAILFNLAALPVALVLMVTGIGAPLVFLFVNAVLL
                     GRELQDMVWLRHRSGPADPAPLTGFERLALGGGIAVLLTVPVVNFVAPFLGAACAVHL
                     IHRKGAIKP"
     gene            3372..4367
                     /locus_tag="VFV30_03735"
     CDS             3372..4367
                     /locus_tag="VFV30_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX4847230.1"
                     /translation="MSKPSTQVIAIGNAIVDVMAPCDDALIERFGLVRGGMTLVDTDR
                     ARELYDAMGPAREISGGSAANTLAGLAALGARCAFIGQVAQDQLGDVFAHDIRAGGIR
                     FDTPARAGDPPTARCLIFVTPDGQRTMNTFLGASQFLPPAALDEAAIQDAEILYLEGY
                     LWDPEEPRAAMRRAIGAARAAGRKVAFTLSDAFVIDRHGDDFRTMIDAGDIDILFANH
                     VELAALTGHDDFHAGIDALKDKVPMLVVTRSEHGAVAATGGAHYEVPAEPVARVVDTT
                     GAGDLFAAGFLHGLVEGRPVADCLTLGAVCAAEIISHFGARPEADLKALAAARLG"
     gene            4510..5442
                     /locus_tag="VFV30_03740"
     CDS             4510..5442
                     /locus_tag="VFV30_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX4847231.1"
                     /translation="MNVVTSIGQFAGRRDVAVDGEHPRVVVADIASRPRGPARIITVA
                     NEKGGVGKSTLAFHLAVALADTGHKVLAVDLDRRQQSLSRAMVARGGTAKRLGARLPL
                     PRHLLLQQPTGAQLCQELARAGWDCDYVVIDAPGHDSPIARRAIAMADMLVSPVNSSF
                     VDLELLGRFHPVTHKLTGPGCFATMVAELRDARSEAGLAPLDWLVVLNRKRRETSHNQ
                     GKIESALRRLAQRMDFRVGAGLYERVAYRELVLLGLTHLDLHRIPDLARTKAVAAAEV
                     MQLLDDLAIAQEAGGLLSDLAGQPLAPAHSSHFG"
     gene            complement(5421..6227)
                     /locus_tag="VFV30_03745"
     CDS             complement(5421..6227)
                     /locus_tag="VFV30_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847232.1"
                     /translation="MKITLVESGCPELSSCLIGYVGGEIAARDHMVRRVIPARPNCFI
                     QIILEGGHGLYDLETREHREAPVAGLFGPLTHYRYDMEVNGPLRTFSARLQPAAAGGL
                     FGVDPIALVDNFVPLDLPASLLETLRAAPDWAGMVEPMDRWLRSLNDGRICADPVARE
                     ATRLRMERGVIPIQDLADAAGLSLRHFQRRFRQLTGLNPKHYARICRIGHAVHRKELE
                     PEAPWTALAHEAGYSDQSHFIRDFKALTGTRPSEFLRGQTPILRYPKWDE"
     gene            complement(6266..7552)
                     /gene="purD"
                     /locus_tag="VFV30_03750"
     CDS             complement(6266..7552)
                     /gene="purD"
                     /locus_tag="VFV30_03750"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX4847233.1"
                     /translation="MNILLIGSGGREHALAWKLAQSRLCDKLFASPGNPGIAEHAALV
                     SLSTADHEAVLAFCAAERIGLVVVGPEAPLVEGLADALRGAGVPVFGPSKAAAQLEGS
                     KAFTKELCARHAIPTAAFRRVASLDAALAALDEFPIPVVIKADGLAAGKGVTVALTRA
                     EAEEAVREIFSGRFGAAGAEAVIEEFLEGEEASLFAITDGHTVVPFGSAQDHKRVGDG
                     DTGPNTGGMGAYSPAPVLTPLLEGEALERIVVPTVKAMADEGMPYSGVLYAGLMLTPR
                     GVQLIEYNCRFGDPECQVLMLRYQGDLAELMLATAENRLGTLPAPRFSDETALTVVMA
                     AEGYPGTPRAGGAIGALDAAEAEGAKVFHAGTARKDGVLVASGGRVLNVTAAGPSVTA
                     AQKTAYRAVKKIHFPDGFCRNDIGWREVRREAMAGE"
     gene            7551..>7723
                     /locus_tag="VFV30_03755"
     CDS             7551..>7723
                     /locus_tag="VFV30_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX4847234.1"
                     /translation="MSPTPYSDEEPAGLVAKEAAGDNAAALSVSEISALLKRTVEDRF
                     GFVRVRGEISGFKR"
BASE COUNT         1207 a         2685 c         2620 g         1211 t
ORIGIN      
        1 ctggtggccg gaattgaaag aacacggagg atcttcgggt gatggacgaa ccgaaattca
       61 ccatcgagga agtcgacagc gtggcgcccc cgccgcgcgc cacggtggag gccgccgaaa
      121 cgccggccga aaagctggag cgcaagcgcg attaccttga tgggttcctg gcccaggcgc
      181 cgcagggcgt ggcccccggc gcgccgggcg gggaccttta cgaaggcgtg attgccgcgc
      241 tgaaggacat tttcgatccg gaaatcccgg tcaacatcta tgacctgggt ctgatctatg
      301 gcgtcgacgt ggccgatgac ggcggcgtgg tggtgaccat gacgctgacc acgccgcatt
      361 gcccggtggc cgaatcgatg ccgggcgagg ttgagctgcg ggtcggcgcg gttcccggcg
      421 tgcgcgatgc cgaagtgaac ctggtctggg atccgccgtg ggatccggcc aagatgagcg
      481 acgaggcccg gctcgaactg gggatgctgt gatgaccgaa accaagatcc gccagcgccc
      541 ggccgccgtg atcctgaccc ccgccgccga agcccgggtg gccaagctga tggcggatgc
      601 gccggccgac gcgatcggcg tcaagctctc gaccccgcgc cggggctgct cggggctggc
      661 ctattcggtc gactatgtca ccgaagcgag cccgctggac gagaagatcg aaacccccgg
      721 cgggctgttc ttcatcgacg gggcttcggt gctctacctg atcggcagca cgatggactg
      781 ggtggaagac gatttcaccg ccggtttcgt gttccagaac ccgaacgcca aagggtcctg
      841 tggctgcgga gaaagtttta cggtatagcg gctgtcatgc cgagtcccga tcccttgccc
      901 ccgtcgaaaa cgcaggcgct gcgcgcgcag atgcgcgggt cgttggacag ggatgaccgt
      961 cgtcttatgg ccgaagctgt cagggccttg cgggatcagc gccgaccgcc cgaaggcggc
     1021 gagccgatcc cggccgttcc gccgcgcggg ccggttcccc tggccggaga cgcggaagcg
     1081 ccgatcgaat agcgatcagc cctgcggccg cggaacgaat gtttcgccgg tttcctcgtc
     1141 gatgaaggcg atcagcgcct tttccatctc gtccagctct tcgtcggcct ccagcatgtc
     1201 gtgcagccac gagtgctcct cgggcgtcag ctggctcgct gcttcggcct cgtcgtccca
     1261 gtcggccaga tagtccggct cgtccccgcc gatcttcagc aggctgccaa agccgtcgac
     1321 atcgggcttg ctggtcatca tccggtgcag gaagccgccg atcccggcgc cctcgctggc
     1381 catgaagtct tccagttcgg ccgcgcgctc ctggctcagc gcctcgtcgc cgccgaagcc
     1441 gagcaggaac tgggccacgc ccttcacgaa caggtcttgc cattcgggcg cattggcttc
     1501 gtaaagggtg gcgtccttga tccggaacag catttcggct tccgccttgc tgaccccggc
     1561 cgggcgctcg ctgccggtgc cgaagatcag gcgcttgatc agcagcgttt cggcagcgtt
     1621 gatcccttcg cgggtcagcg agccatggcg ggtcgggcct tcgccaccca ccaccgcgtc
     1681 ctcgatctgg cgcagggcat aggcgcgcag gctggccggc acgctggtgg cggtttcgat
     1741 caccttcacc agcagctcca gctcggccag cgtgccgaca tggccatccc ggtcgatccg
     1801 cgagacgagc tcgtcggcca tttcctgatc gacataaccg cgcggttcca cggtgtggac
     1861 cacgaagctg gtcagcgcct cgacaaagaa gtcgcaccac tcggcgctcg gctcgtcgca
     1921 ggtgtcgttg gcaacgaaca gcgattcggc ctcatccggg ctcatcttgc cgtcggccca
     1981 gccgagacgg cgcaattcga ggatttcttc ggccgaaata tggccgtcgc ccgcgacttg
     2041 cgtggtgaga ttaccgaatt ccaagctcat ggcgcacagg cccccatgca aaaaacagag
     2101 ggtccttgtg ccgaaataac cttaaatccg ggttacccct gtgtggatac cgtggcgtgc
     2161 aaggatgcct gttcagcgcc cgcgcagcgc gttgacgcag gatccgcggt ccacgtcctg
     2221 cccgtcgctg gcgcggcgcg cctcgacata ggtcgcttcg ggctcgcgcc ccggggccgg
     2281 gggatagagg tagacgtcca gaacgcaggc cgtcccggtg aactgcagct tgcgggcatc
     2341 gccttcccac acttccagcc gggccgggcc gaactggcgg accagatcgg cattggtggc
     2401 gccgatcacc ccttccaggc ccggtgcggt cagcacccgg gggcgcggcg gcggggcctg
     2461 gaccggcacg cgcaccggca cccggcccgc ggtttgcggg gccggccggg cggtggtggt
     2521 gccgcccgac gcacaggcgg acagcagcag gccaagggcc aggacgggga cggggcggaa
     2581 agtcatggtt tgatcgcccc cttgcgatga atcaggtgca ccgcgcaagc cgcgcccagg
     2641 aatggtgcga cgaaattgac caccggcacg gtgagcagaa ccgcgatccc tccgcccagc
     2701 gccagccgtt cgaacccggt cagcggggcc ggatcggccg ggccgctgcg gtggcgcagc
     2761 cagaccatgt cctgcagctc gcgccccagc agcaccgcgt tgacgaacag gaagaccagc
     2821 ggcgcgccga tccccgtgac catcagtacc agggcaacgg gcagggctgc caggttgaac
     2881 aggatcgccc gtgccgcacc ggccagcgcc cgccgggcct cttccgccag gcccagcttg
     2941 cgggcccggg cggccatgtc cggatagtcg cgcgcctcga tcgcgtggac cacctcgtcg
     3001 gcgaagaact gcagcacggc cagggccagg atccgccaca gcagcccgcc ggcaatcagc
     3061 acgatcagca gggccaggac gccgctgtaa ccgtggctca tgccatagga gcgcgcgtaa
     3121 cggtccagga cgccggccag accgtaccat ccggcgctgc ccagcgcggc aaagacgagc
     3181 agcgtcacgg ccaggctttt cagccagatc ggcagcaggt ggcggtcggc catccggggc
     3241 agggcaaggg caagcgcctg aagcatgacc gggccatcgc ctgccccccg ggcgctgtca
     3301 acgcttccct gcgcggtgaa aagcggctag gcgcggcgga cttttcgccc gtctttcagg
     3361 aacacccgcc catgtccaag ccgtccaccc aggtcatcgc catcggcaat gcgatcgtcg
     3421 atgtcatggc cccgtgcgac gatgcgctga tcgagcggtt cgggctggtc cgcggcggga
     3481 tgaccctggt cgacaccgac cgcgcccgcg agctttacga cgcgatgggc ccggcgcgcg
     3541 agatttcggg cggttcggcg gccaacaccc tggccggcct cgccgcgctg ggcgcgcgct
     3601 gcgccttcat cggtcaggtc gcgcaggacc agctgggcga tgtcttcgct cacgatatcc
     3661 gggccggcgg gatccgcttc gatactccgg cgcgggccgg cgatccgccg acggcgcgct
     3721 gcctgatctt cgtcaccccc gatggccagc ggaccatgaa caccttcctc ggtgcctcgc
     3781 agttcctgcc gcccgccgcg ctggatgaag cggcgatcca ggacgcggag atcctctacc
     3841 tcgaaggcta tctgtgggat ccggaagagc cgcgcgcggc gatgcgccgg gcgattggcg
     3901 cggcccgggc ggccgggcgc aaggtggcct tcaccctgtc cgacgccttc gtgatcgacc
     3961 gccacggcga cgatttccgc accatgatcg atgccggcga tatcgacatc ctgttcgcca
     4021 accatgtcga actcgcggcg ctgaccggcc acgacgattt ccacgccggg atcgatgccc
     4081 tgaaggacaa ggtgccgatg ctggtggtga cccgcagcga gcacggcgcg gtcgccgcca
     4141 ccggcggcgc gcattacgaa gtcccggccg aaccggtcgc gcgggtggtc gacaccaccg
     4201 gcgcgggcga cctgttcgcc gcagggttcc tccacggact ggtcgaaggc cgtccggtgg
     4261 ccgactgcct gaccctgggc gcggtttgcg cggcggagat catctcgcac ttcggggccc
     4321 gtccggaagc cgatctcaag gcgctggccg ccgcccggct gggctgaacc gacggggccg
     4381 gctgacgtta cgtcactgcc ggtccgttaa ctttatccga ttcgatatcc acaggttttt
     4441 ccactaagct ggtcgcttcg ggcgattcgc agccgcgccc agacgcggcg gaaaacgggg
     4501 gtctgtggga tgaacgtggt cacttcgatc gggcagttcg ccgggcgccg cgacgtggct
     4561 gtggatggag agcatccccg cgtggtcgtg gccgatatcg ccagccgccc gcgcggtccg
     4621 gcccggatca tcaccgttgc caacgaaaag ggcggcgtcg gcaagtcgac cctggccttt
     4681 cacctcgccg ttgccctcgc cgataccggg cacaaggttc tggcggtcga tctggaccgc
     4741 cgccagcagt cgctcagccg ggccatggtg gcccgcggcg gcacggccaa gcggctgggc
     4801 gcgcggcttc cgctgccccg ccacctgctg ctgcaacagc cgaccggcgc gcagctgtgc
     4861 caggagctgg cccgggccgg gtgggactgc gactatgtgg tgatcgacgc cccggggcac
     4921 gacagcccga tcgcgcgccg cgccatcgcc atggccgaca tgctggtctc gccggtcaat
     4981 tcctcgttcg tggatctgga actgctcggc cggtttcatc cggtcaccca caagctgacc
     5041 ggccctggct gctttgccac catggtcgcc gaactgcgtg atgcgcggtc cgaggccggg
     5101 cttgctccgc tggactggct ggtggtgctc aaccgcaagc ggcgcgaaac cagccacaac
     5161 cagggcaaga tcgaatccgc cctgcggcgc ctggcccagc ggatggactt ccgggtcggc
     5221 gcgggactgt acgagcgggt cgcttaccgc gagctagtcc tgctggggct gacccacctc
     5281 gacctgcacc ggattcccga tctggcccgg accaaggccg tggccgcggc cgaagtgatg
     5341 cagttgctgg acgacctggc gatcgcccag gaggccggcg ggctgctatc cgatctggcc
     5401 ggacagccgc tcgcgccggc tcactcgtcc cacttcggat agcgcaggat cggggtctgc
     5461 ccgcgcagga actcgctcgg gcgggttccg gtcaaggcct tgaaatcccg gatgaagtgc
     5521 gattggtcgg aatagcccgc ctcgtgcgcc agcgcggtcc agggcgcttc cggttccagc
     5581 tccttgcggt ggacggcgtg gccgatccgg caaatccggg catagtgctt gggattgagc
     5641 ccggtcagct ggcggaagcg gcgctggaaa tgccgcagcg acagcccggc cgcatcggcc
     5701 aggtcctgga tcgggatcac cccgcgctcc atccgcagcc gggtggcttc gcgggcgacc
     5761 ggatcggcgc agatccgccc gtcgttgagc gagcgcagcc agcggtccat cggttccacc
     5821 atgccggccc agtccggcgc ggcgcgcagc gtttcaagca ggctggccgg caggtcgagc
     5881 ggcacgaagt tgtcgaccag ggcgatcgga tcgaccccga acagcccgcc ggccgcggcc
     5941 ggctgcaacc gggccgaaaa ggtccgcagg ggcccgttca cttccatgtc atagcggtaa
     6001 tgggtcagcg ggccgaacag cccggcgacc ggcgcttcgc ggtgttcgcg ggtctccagg
     6061 tcgtagagcc cgtgcccgcc ttccagaatg atctgaatga aacagttggg ccgggccgga
     6121 atgactcgcc ggaccatgtg atcgcgggcc gcgatttcgc ccccgacata accgatcaga
     6181 caggagctga gctccggaca gccgctttca accagggtaa tcttcacccc gtttccccta
     6241 ttgtgcgcgc gccctgacgt tcttgttatt cgcccgccat agcctcgcgg cgcacctcgc
     6301 gccagccgat atcgttgcgg cagaaaccgt cggggaaatg gatttttttg accgcccgat
     6361 aggccgtctt ctgggccgcg gtgaccgacg gcccggccgc cgtgacgttg agcacgcgcc
     6421 cgccgctggc caccaggacc ccgtccttgc gggcggtgcc ggcgtggaac accttcgcgc
     6481 cctcggcttc ggctgcgtcc agcgcgccga ttgcgccgcc cgcccggggc gtgccgggat
     6541 agccttcggc cgccatgacc acggtcagcg cggtttcgtc cgaaaagcgc ggcgcgggca
     6601 gggtgcccag gcggttttcg gccgtggcca gcatcagttc ggccaggtcg ccctgatagc
     6661 gcagcatcag cacctggcat tcgggatcgc cgaaccggca attgtattcg atcagctgga
     6721 cgccccgcgg ggtcaacatc agcccggcat agagcacgcc ggaatagggc atgccctcgt
     6781 ccgccatggc cttgaccgtg ggcaccacga tccgctccag agcctcgccc tcgagcagcg
     6841 gggtcagcac cggggccggg ctgtaggcgc ccatcccgcc ggtgttgggc ccggtatcgc
     6901 cgtcgcccac ccgcttgtgg tcctgggccg atccgaacgg gaccacggtg tgcccgtcgg
     6961 tgatcgcaaa caagctcgcc tcctcgccct ccaggaactc ctcgatcacc gcttcggccc
     7021 cggccgcacc gaaccggccg gagaagatct cgcgcaccgc ctcctcggcc tcggcccggg
     7081 tcagggccac ggtcacgccc ttgccggcgg ccaggccatc ggccttgatc accaccggga
     7141 tcgggaattc gtcgagcgcg gccagcgccg catcgagact ggcgacccgc cggaacgcgg
     7201 cggtcggaat ggcgtggcgg gcgcagagct ccttggtgaa ggctttcgag ccttcgagct
     7261 gcgcggcggc cttcgacggc ccgaacaccg gcacgcccgc gccgcgcagg gcatcggcca
     7321 ggccctcgac cagcggcgcc tcggggccga ccacgaccag cccgatccgc tccgccgcgc
     7381 aaaaggccag cacggcctcg tgatccgccg tggacagcga aaccagcgcg gcatgctcgg
     7441 caatcccggg gtttccgggg cttgcaaaca gcttgtcgca cagccgggat tgcgccagct
     7501 tccaggccag cgcatgttcg cgcccgcccg aaccgatcag caggatattc atgtcgccga
     7561 cgccctattc cgatgaagaa cccgccgggc tggtagcgaa ggaagcggcg ggcgacaacg
     7621 ccgcagcgct ctcggtcagc gagatttccg cattgctgaa gcgcacggtc gaggaccgct
     7681 tcggcttcgt gcgggtgcgg ggcgagatca gcgggttcaa gcg
//
LOCUS       DASUDV010000179         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1198567,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000179 DASUDV010000000
VERSION     DASUDV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198567"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFV30_03760"
     CDS             <1..759
                     /locus_tag="VFV30_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4847235.1"
                     /translation="GSDEYRVGEIFLSANSENRDQVLQNALRIVDQIKKGGSFVGYAR
                     QYSEASTAPVGGDLGWIRAGVLPGELDQVVQALQPGQLAGPIEVRGGFSIILLIDKRK
                     VLTADPRDATLSLKQISITFAPGTTEAQATQKAQEFAATMKSAKGCAAVDPIAAQMGA
                     EVVTNESIKARDLPGALQEMVLGLSLGETTPPFGSIQDGVRVLMLCGRDDPKVSGGPN
                     FDEVQAQLEDERINRRAQTYLRDLRRDAVIDYGN"
     gene            766..1779
                     /gene="pdxA"
                     /locus_tag="VFV30_03765"
     CDS             766..1779
                     /gene="pdxA"
                     /locus_tag="VFV30_03765"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX4847236.1"
                     /translation="MDGAPLAVSLGDPAGVGPELIAAAWAARETDALPPFVVTGGADV
                     LRAAAARRGLAVPVQPVFHPNEAAACFGHALPVLDGADGDYQPGQPDRAGSELALASL
                     QEATGLARAGEVAGIVTGPVSKARLADVGFDHPGQTEFVAYACGYAPGDAVMMLAGPN
                     LRTVPLTVHIPLIEVAPTLTASLIERKARIVAMALARDFGIERPRIAVAGLNPHAGED
                     GRMGSEEATIIAPAIAALQAEGLDVTGPHPADALFTARTRETYDVALCMYHDQALIPL
                     KALDFDQGVNVTLGLPVIRTSPDHGTAFGIAGTGQAHAGAMIAAIRMAGECAAWRYGE
                     CDD"
     gene            1772..>2091
                     /locus_tag="VFV30_03770"
     CDS             1772..>2091
                     /locus_tag="VFV30_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEX4847237.1"
                     /translation="MTDLPPLRDVINRHGLFASKALGQNFLFDEQLLDRIAAIPGKLK
                     GAQVLEVGPGPGGLTRALLRAGAQVTAIEMDRRCLPALAELSEAFPGQLRVIEGDATK
                     VDHGL"
BASE COUNT          330 a          754 c          714 g          293 t
ORIGIN      
        1 ggcagcgacg aataccgcgt cggcgaaatc ttcctctcgg ccaacagcga aaaccgcgac
       61 caggtgctgc agaacgcgct gcggatcgtc gaccagatca agaagggcgg cagctttgtc
      121 ggctatgccc gccagtactc cgaagcttcg accgcgccgg tcggcggcga tctcggctgg
      181 atccgcgccg gcgtgctgcc gggcgagctg gaccaggtgg tccaggcgct gcagcccggc
      241 cagctggccg gcccgatcga agtgcgcggc ggcttctcga tcatcctgct gatcgacaag
      301 cgcaaggtgc tgaccgccga tccgcgcgac gccacgctca gcctcaagca gatctcgatc
      361 accttcgccc cggggacgac cgaagcccag gcgacccaga aggcgcagga attcgccgcc
      421 accatgaaga gcgccaaggg ctgcgccgcg gtcgatccga tcgccgcgca gatgggcgcc
      481 gaagtggtga ccaacgaatc gatcaaggcc cgcgacctgc ccggcgcgct gcaggaaatg
      541 gtccttggcc tgtcgctggg tgaaaccacg ccgccgttcg gctcgatcca ggacggcgtg
      601 cgcgtgctga tgctgtgcgg gcgcgacgat cccaaggtct cgggcggccc gaacttcgac
      661 gaggtgcagg cccagctgga agacgagcgg atcaaccgcc gcgcccagac ctatctgcgc
      721 gaccttcgcc gcgacgcggt gatcgattac ggtaactaac cagcaatgga cggggcgccg
      781 ctcgcggtat cgctgggcga cccggccggc gtcggccccg aactgatcgc cgcggcctgg
      841 gccgcgcgcg agacggacgc cttgccgccc ttcgtggtga ccggcggagc ggacgtcctg
      901 cgcgccgccg ctgcccggcg cggcctggcc gtgccggtcc agccggtctt tcatcccaac
      961 gaggcggcag cctgtttcgg ccatgccctg ccggtgctgg acggcgccga cggcgattac
     1021 cagccgggtc agccggaccg cgcgggttcg gaactggcgc tggccagcct gcaggaagca
     1081 accggcctgg cccgagccgg cgaagtggcc ggaatcgtga ccgggccggt atccaaggcg
     1141 cggcttgccg atgtcgggtt cgaccatccc gggcagaccg aattcgtcgc ctatgcctgc
     1201 ggttatgccc ccggcgatgc ggtgatgatg ctggccggcc ccaacctgcg caccgtgccg
     1261 ctgaccgtgc acatcccgct gatcgaagtg gcccccaccc tcaccgcctc gctgatcgag
     1321 cgcaaggccc ggatcgtcgc catggccctg gcccgcgatt tcgggatcga gcgcccgcgg
     1381 atcgcggtgg ccgggctcaa cccccatgcc ggcgaagacg gccggatggg gagcgaggaa
     1441 gcaacgatca tcgccccggc catcgccgcg ctgcaggccg agggcctcga cgtgaccggc
     1501 ccgcacccgg ccgacgcgct gttcaccgca cggacgcgcg aaacctatga tgttgcgctg
     1561 tgcatgtacc acgatcaggc gctgatcccg ctgaaggcgc tcgatttcga ccagggggtc
     1621 aacgtgaccc tgggcttgcc ggtgatccgc accagcccgg accacggcac cgcctttggc
     1681 attgccggca ccggccaggc ccacgccgga gcgatgatcg ccgccatccg catggccggc
     1741 gaatgcgccg cctggcgcta tggcgagtgc gatgactgac cttcccccgc tgcgcgatgt
     1801 catcaaccgc cacggcctgt tcgccagcaa ggcgctgggc cagaacttcc tgttcgacga
     1861 gcagctgctc gaccggatcg ccgcgatccc cggcaagctg aagggcgcgc aggttctgga
     1921 ggtcggcccc ggccctggcg gcctgacccg cgcgctgctc cgcgccggag cgcaagtgac
     1981 cgcgatcgag atggaccggc gctgcctgcc cgccctggcc gaactgagcg aggccttccc
     2041 cggccagctg cgggtgatcg agggcgatgc caccaaggtc gatcacggcc t
//
LOCUS       DASUDV010000180         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_72141,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000180 DASUDV010000000
VERSION     DASUDV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72141"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFV30_03775"
     CDS             complement(<1..415)
                     /locus_tag="VFV30_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847238.1"
                     /translation="MAPAQGPGPVLQDRLAAFALVALAVGAGLAAFWLGGAPAAYLPV
                     NAAALALAAAAFLIPGERASPVLRTLILIAAPLLIGATFLIGPEVLGVHRWIALGPLL
                     LHPGSLLLPALVTVAALQSDRAALAVVAVCAGLVWL"
     gene            complement(417..1823)
                     /gene="lpdA"
                     /locus_tag="VFV30_03780"
     CDS             complement(417..1823)
                     /gene="lpdA"
                     /locus_tag="VFV30_03780"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX4847239.1"
                     /translation="MTEQTYDYDVIFIGAGPGGYVGAIRAAQLGLKTACVESRETLGG
                     TCLNVGCIPSKALLHGSEKFEEAANGTLARYGVKLGKVELDLAALQADKADAVKGLTG
                     GIEFLFKKNKVDWLKGTGTFKDAHTVTVAGKDYTAKEVVIATGSSVTPLPGVEVDNDK
                     GVIVDSTGALALTKVPGHMVVIGGGVIGLEMGSVWRRLGAKVTVVEFLDALLPGMDMD
                     VRKDAAKIFKKQGMELKLGTKVTGAVVKGKKATLTVEPAKGGAAETIEADCVLVAIGR
                     RPNVAGLGLENIGLELNARGQIETGHDFRTSVDGVRAIGDVIPGPMLAHKAEDEGIAV
                     AEYIATGTGIVNHAVIPGVVYTTPEFAGVGLTEEQAREQGEIKVGKFPMMANSRAKTN
                     HEPDGFVKVIADAKTDKVLGVWAIATVAGTMIAEAALAMEFGATSEDIAYTCHAHPTH
                     SEAVKEAAMAVTGKPIHI"
     gene            complement(1826..>2577)
                     /locus_tag="VFV30_03785"
     CDS             complement(1826..>2577)
                     /locus_tag="VFV30_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4847240.1"
                     /translation="PDEVIAGCNAALSEYKFNPDQKAILLNRRGVAYHKTGQFALALA
                     DFTQAIEIDPNQVLFYNRGNVRDRMDDVPGAIADFSSAIERQPDFVSAISSRGILYVH
                     SRQPAKSIDDFTRVIALRPGSSDAYFNRGMAYSIAGQPDRALPDFNAALERNPDNLDA
                     LMSRVSVNTQLGNEPAVIADLDRAAQLAPNRADIFAKRCFYWRRQKQDAKADADCATA
                     SRLDPSLAAQLTRGYKGEIRLAHQVGLKRVD"
BASE COUNT          389 a          868 c          861 g          459 t
ORIGIN      
        1 gcagccagac cagccccgcg cagacggcca ccacggccag cgcggcccgg tcgctctgca
       61 gcgccgccac ggtgacgagc gcgggcagca gcagcgatcc gggatggagc agcagcggtc
      121 ccagcgcaat ccagcggtga acgcccagca cttcgggccc gatcaggaac gtggctccga
      181 tcagcagcgg ggcggcgatc aggatcaggg tccgcaagac cgggctggcg cgctcacccg
      241 ggatcagaaa ggctgccgcg gccagcgcaa gcgcggcggc gttgaccggc agataggcgg
      301 ccggcgcccc gccgagccag aaggcggcca ggccggcgcc cacggccagg gcgaccagcg
      361 cgaaggctgc cagcctgtcc tgcaggaccg ggccgggccc ttgcgccggg gccatgtcag
      421 atgtggatcg gcttgccggt aacggccatc gccgcttcct tgaccgcttc cgaatgggtc
      481 gggtgggcgt ggcaggtgta ggcgatgtcc tcgctggtcg cgccgaattc catcgccagc
      541 gcggcttcgg cgatcatcgt gccggccacc gtggcaatcg cccagacgcc cagcaccttg
      601 tcggtcttgg cgtcggcgat gactttcacg aaaccgtccg gctcgtggtt ggtcttggcg
      661 cggctgttgg ccatcatcgg gaacttgccg accttgatct cgccctgctc cctggcctgc
      721 tcctcggtca ggccgacacc ggcgaattcg ggggtggtgt agaccacgcc cgggatcacc
      781 gcgtgattga cgatcccggt gccggtggcg atgtattcgg ccacggcaat gccttcgtcc
      841 tcggccttgt gggccagcat cgggccgggg attacgtcgc cgatcgcgcg cacgccgtcc
      901 acgctggtgc ggaagtcatg gcccgtttcg atctggccgc gcgcgttcag ctcaagcccg
      961 atgttttcca ggcccagccc ggccacgttg ggccggcggc cgatcgccac cagcacgcag
     1021 tcggcttcga tcgtttcggc cgcgccgccc ttggccggct cgaccgtcag cgtcgccttc
     1081 ttgcccttga ccaccgcgcc ggtcaccttg gtgcccagct tcagctccat gccctgcttc
     1141 ttgaagatct tggcggcgtc cttgcgcaca tccatgtcca tgccgggcag cagcgcgtcg
     1201 aggaattcga ccacggtgac cttggccccc aggcgccgcc agaccgagcc catttcgagg
     1261 ccaatcacgc cgccgccgat caccaccatg tggcccggca ccttggtcag cgccagcgcg
     1321 ccggtggaat cgacgatcac gcccttgtcg ttatcgacct ccacgcccgg cagcggggtg
     1381 acgctggagc cggtggcgat gacgacttcc ttggcggtgt agtccttgcc cgcaacggtc
     1441 acagtgtggg catccttgaa ggtgccggtg cctttcagcc aatcgacctt gttcttcttg
     1501 aacaggaact cgatcccgcc ggtcaggccc ttgaccgcat cggccttgtc cgcctgcagc
     1561 gcggcgagat cgagctcgac cttgccgagc ttcacgccat agcgggcgag ggtgccgttg
     1621 gcggcttcct cgaacttctc cgaaccgtgc agcagtgcct tcgaggggat gcagccgacg
     1681 ttgaggcagg ttccgcccag cgtctcgcgg ctttccacgc aggccgtctt cagccccagc
     1741 tgcgccgcgc ggatcgcgcc gacatagccg ccggggccgg ccccgatgaa gatcacgtcg
     1801 tagtcatagg tctgttcggt catgttcaat ccacgcgttt gaggccaacc tgatgcgcaa
     1861 ggcgaatttc gcccttgtag cccctggtca gttgagcggc caggctgggg tccagccggg
     1921 aagcggttgc gcagtccgcg tccgcctttg cgtcctgctt ttggcggcgc cagtagaaac
     1981 accgcttggc gaaaatatcg gcccggttcg gggccagctg cgcggcccga tcaaggtcgg
     2041 cgatcacggc gggctcgttg cccagctggg tgttgaccga gacccgcgac atcagcgcgt
     2101 cgaggttgtc cggattgcgt tcaagcgccg cgttgaaatc gggcaatgcg cgatcgggct
     2161 ggccggcaat gctgtaggcc atgccccggt tgaaataggc gtcgctcgaa cccgggcgca
     2221 gcgcgatgac ccgggtgaaa tcgtcaatcg acttcgcggg ttgacggctg tggacataaa
     2281 ggatgccgcg cgagctgatg gcgctgacga aatcgggctg gcgttcgatc gcgctgctga
     2341 aatcggcgat ggcgcccggc acatcgtcca tcctgtcgcg cacgttgccg cggttgtaga
     2401 acagcacctg attgggatcg atttcgatgg cctgggtgaa gtcggccagc gccagcgcaa
     2461 actggccggt cttgtgatag gccaccccgc ggcggttgag caggatcgcc ttctggtccg
     2521 ggttgaactt gtattccgaa agcgcggcgt tgcagcccgc gatcacctcg tcgggct
//
LOCUS       DASUDV010000181         6468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_204588,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000181 DASUDV010000000
VERSION     DASUDV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204588"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..139
                     /locus_tag="VFV30_03790"
     CDS             2..139
                     /locus_tag="VFV30_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX4847241.1"
                     /translation="MVLLGLAFTGHYTPWTWLGIVPLLTGLVGWCPPYSLLGINTCKA
                     R"
     gene            complement(264..1772)
                     /gene="tig"
                     /locus_tag="VFV30_03795"
     CDS             complement(264..1772)
                     /gene="tig"
                     /locus_tag="VFV30_03795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX4847242.1"
                     /translation="MQIVETSNEGLKRAYTVTIKAGDIAARIDGEVSKIAPQVRMPGF
                     RPGKVPANLVKKMHGPALHQEALNTSVREALDKVIADNKLRPAMQPQVALGEGYEEGK
                     DAELTVSLEVLPTIPTPSIEGIKLEKLVVPVDEAEVDEQLAKLASGAKSFTDAKKGKK
                     AENGDQLIIDFVGKLDGVAFEGGSATDTALELGAGRFIPGFEEQLVGVKAGDEKTITV
                     TFPADYPAENLKGQDATFDITVKAVKVPGESKADDDFAKSLGLESLEQLKGLLRGQLE
                     QESAGLTRTQMKRSLLDLLAADHDFDVPPSMVEAEFQQIWAQLQQEAQNEEDPAAALK
                     EIEDEKDDYRGIAVRRVRLGLLLSEIGQANGVEVTQQEMQMLVTQAAQQYRPEDRQRF
                     VEFIQQDPLAAAQLRAPLYEDKVVDFLFDKAEVTERAVTKDELQAAIEAEEAAPAPAK
                     AKKAPSKKAAKADDKPAKEAAPKAEKKAAAPKAEKAEKAEKKPAAKKAPAKK"
     gene            complement(1829..1913)
                     /locus_tag="VFV30_03800"
     tRNA            complement(1829..1913)
                     /locus_tag="VFV30_03800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1877..1879),aa:Leu,seq:tag)
     gene            complement(1991..3871)
                     /gene="sppA"
                     /locus_tag="VFV30_03805"
     CDS             complement(1991..3871)
                     /gene="sppA"
                     /locus_tag="VFV30_03805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX4847243.1"
                     /translation="MAFARKVWNLLVAIKDGLVLIFLLLFFMALYGVLMSRPSPAAMR
                     DGALVLRLNGTVVEEPATPDPLEGLLTGQAQPREHRARDVIRAIGLAGEDERVKAVVL
                     DLSEFMGGGMVTLQEIGNALDKVRAKGKPVLAYSMAYTDDGMLLAAHASEVWVHPLGG
                     AFVMGPGGDQLYFGGLLQRLKVKVHVYKVGTYKDFVEPYLLDKASESSRQARGAVLSA
                     LWEEWQADVAKARPKANLALVTRDPVGWLKGAGGDTAKAALSAGLVDKLGDEVAFGER
                     VKAIAGEDRFDERPGSYAHNSPDALLAAHPAKDDGEAIAVVTVAGALVDGKAGPGTAG
                     GERIARLIDEATASEAKALVLRVDSQGGSVFASEQIRLALERFKAKGRPIAVSMVNMA
                     ASGGYWVSTPAKRIFAQPGTVTGSIGIFAMLPSFEQTLGQYGVTSDGQSTTPLSGQPD
                     VLGGISPEADAMIQASIENGYARFLGLVGKARGKTPEQIDAIAQGRVWDGGTARQNGL
                     VDQFGGLDEALAWAAKEAKLEEWHPEFYGTEADPYGSLLDRLLGGEEEDGQAPAADFA
                     GLMAQRQEARLAGGLAQVGWLLGTRGAQAYCLDCPVPLAGKPAPRGGGWLANLAKVLS
                     LR"
     gene            4036..5028
                     /locus_tag="VFV30_03810"
     CDS             4036..5028
                     /locus_tag="VFV30_03810"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX4847244.1"
                     /translation="MTSPNANSAGRYPAGSAAFPHRDLTGLAGLAPWEILFLLDEAEQ
                     WVELNRQPHKRDDRLAGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMLVAQSSVKK
                     GETLIDTAMTLNAMHADAMVIRHQSSGAVRLIAEKVDCPVLNAGDGQHEHPTQGLLDA
                     LTIRKAKGDFGGLRVTICGDILHSRVARSNIHCLTTLGAEVRVCAPPALMPAGIEAMK
                     VTPFHDFDAALKGADVVMMLRLQQERMSGQFIPSPREYRHLYGLTLDRLAKAAPDALV
                     MHPGPMNRGVEIDSNVADLAGRSAITTQVEMGVAMRMACLDVLTRRARGVEGWA"
     gene            5025..6257
                     /locus_tag="VFV30_03815"
     CDS             5025..6257
                     /locus_tag="VFV30_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4847245.1"
                     /translation="MSAPRPLTIVGGQLVLPGTRPVPGTLRCADGRIQAIGGAEPGPD
                     DLVIDARGALVAPGLVDLGVFAIDKPACHFGGITRAALMPDQGVVLDKPSAVRFAAQS
                     GKPDLWVHPLGAATRDLAGTELAELALMRDAGAKAVATGRRWIADSGTMHRLLSYCAM
                     LGLVVVSHAEDGGLAGSAVATAGEMATRLGLPSAPAEAEALAVARDIALAELTGARLH
                     FRQVTTRAALALVRAARARGVAVSAGVTPAHFMLSDVSIGDFRTFTRMSPPLRSEDDR
                     QAVREALADGTISVIASGHDPRGPEDKRLPFADAEPGMAGAETLLALTLGLVCDGVIG
                     TARAFELLCTNPARILGVEAGRLEVGAEADIAVIDPDKPWVVDSDKMAAAAGNTPFDK
                     QGVQGRVLALFKGGVPVG"
BASE COUNT          952 a         2282 c         2146 g         1088 t
ORIGIN      
        1 gctggtgctg ctgggcctgg cctttaccgg ccactacacc ccgtggacct ggctgggcat
       61 cgtgccgctg ctgaccggcc tggtcggctg gtgcccgccc tattcgctgc tgggcatcaa
      121 cacctgcaag gcgcgctgac cactgggtcc cgccggccgt cgggtcgcaa ggccggcgac
      181 aaacgaaaag gcctggcgcg atgggtatcg cgccaggcct tttcacaccg ggccctgctg
      241 aaaccagaca gggccggatc ggattacttc ttggccgggg ccttcttggc agccggcttc
      301 ttctcggcct tttcggcctt ctcagccttg ggcgccgcgg ccttcttctc ggccttggga
      361 gccgcttcct tggccggctt gtcgtcggcc ttggcggcct tcttggacgg agccttcttg
      421 gccttggccg gagccggagc ggcttcttcc gcttcgatcg cggcctgcag ctcgtccttg
      481 gtcacggcgc gctcggtgac ttcggccttg tcgaacagga agtcgacgac cttgtcttca
      541 tagagcggcg cgcgcagctg ggcggcggcc agcggatcct gctggatgaa ttcgacgaac
      601 cgctggcggt cttccgggcg gtactgctgg gcagcctggg tgaccagcat ctgcatttcc
      661 tgctgggtca cttcgacgcc gttggcctgg ccgatttccg acagcagcag gcccaggcgc
      721 acgcggcgca cggcgatccc gcggtagtcg tccttctcgt cctcgatttc cttcagcgcg
      781 gcggccgggt cttcctcgtt ctgggcttcc tgctgcagct gggcccagat ctgctggaat
      841 tcggcttcga ccatgctggg gggcacgtcg aaatcgtgat cggcggcgag caggtcgagc
      901 agcgagcgct tcatctgcgt gcgggtcagg ccggcgcttt cctgctccag ctggccgcgc
      961 agcaggccct tgagctgctc caggctttca aggcccagcg acttggcaaa gtcatcgtcg
     1021 gccttcgatt cgccgggcac cttcaccgcc ttgacggtga tgtcgaaagt ggcgtcctgg
     1081 cccttcaggt tctcggccgg atagtcggcc gggaaggtca cggtgatggt cttctcatcg
     1141 ccggccttga cgcccaccag ctgctcttcg aagccgggga tgaaacggcc cgcgcccagt
     1201 tccagcgcgg tgtcggtggc gctgccgcct tcaaaggcca cgccatccag cttgccgacg
     1261 aaatcgatga tcaactgatc gccgttttcg gccttcttgc ccttcttggc gtcggtgaag
     1321 ctcttcgcgc ccgaggccag cttggccagc tgctcatcca cttcggcttc gtccaccggc
     1381 acgaccagct tttccagctt gatgccttcg atcgacgggg tcgggatggt cggcaggact
     1441 tccaggctga cggtcagctc ggcatccttg ccttcctcat agccttcgcc cagcgccacc
     1501 tgcggctgca tcgccggacg cagcttgttg tcggcgatca ccttgtccag cgcttcgcgg
     1561 accgaggtgt tgagcgcttc ctgatgcagg gccgggccgt gcatcttctt gaccaggttg
     1621 gccggaacct tgccggggcg gaagccgggc atgcgcacct gcggcgcgat cttggacact
     1681 tcgccgtcga tccgggcggc aatatcgccg gccttgatcg tgacggtgta agcgcgcttc
     1741 aaaccctcgt tgctggtctc gacaatctgc atgacaaaag aagccttctg gttactcgct
     1801 gcacttcgcc ggcagggccg ggccgaactg gtgcgggcga agggactcga acccccacat
     1861 cttgcgatac tggtacctaa aaccagcgcg tctaccagtt ccgccacgcc cgcaggaccc
     1921 gacgaagtgt tgaatcaagc ggtggccctt agtggcggta cgggcaaagg gcaagcacta
     1981 gcgcagcgtt tcagcgcaag gacagcacct ttgccagatt cgccagccag ccgcccccgc
     2041 gcggcgccgg tttgccggcc agcggcaccg ggcaatcgag gcaataggcc tgcgcgccgc
     2101 gggttcccag cagccaccca acctgggcca ggccgcccgc cagacgggcc tcctggcgct
     2161 gcgccatcag cccggcgaaa tcggcggccg gggcctggcc gtcctcttcc tcgccgccca
     2221 gcagccggtc gagcagcgag ccataggggt ccgcctcggt cccgtagaat tcgggatgcc
     2281 attcttccag cttggcctcc ttggcggccc aggccagcgc ctcgtccagc ccgccgaact
     2341 ggtcaaccag cccgttctgc cgcgcggttc cgccgtccca gacccgccct tgcgcgatcg
     2401 cgtcgatctg ttccggggtc ttgccgcggg ccttgccgac caggccgagg aaccgggcat
     2461 agccgttctc gatactggcc tggatcatcg cgtcggcctc cgggctgatg ccacccagca
     2521 catcgggctg cccggaaagc ggggtggtcg attgcccgtc cgaggtcacg ccgtactggc
     2581 ccagggtctg ctcgaagctg ggcagcatcg cgaagatccc gatcgagccg gtcaccgttc
     2641 cgggctgggc gaagatccgc ttggccggag tggaaaccca atagccgccg ctggccgcca
     2701 tgttgaccat cgagaccgcg attggccgcc ccttggcctt gaagcgttcc agcgcgaggc
     2761 ggatctgttc ggaggcgaag accgatccgc cctgcgaatc gacccgcagc accagcgcct
     2821 tggcctcgct ggcggtggcc tcgtcgatca gccgggcgat ccgctcgccg ccggccgtgc
     2881 cgggcccggc cttgccgtca accagcgcgc cggccacggt gaccaccgcg atcgcctcgc
     2941 catcgtcctt ggccggatgc gcggccagca gcgcatcggg gctgttgtgg gcatagctgc
     3001 ccgggcgctc gtcaaagcgg tcttcaccgg caatcgcctt cacccgctcg ccaaaggcca
     3061 cttcgtcgcc cagcttgtcg accagtccgg ccgaaagcgc ggccttggcg gtgtcgccgc
     3121 ccgcaccctt cagccagccg accggatcgc gggtgaccag cgccagattg gccttgggcc
     3181 gggccttggc gacatcggcc tgccattcct cccacagcgc cgaaagcacc gccccgcgcg
     3241 cctggcgcga ggattcggag gccttgtcga gcagataggg ttcgacgaaa tccttgtagg
     3301 tgccgacctt gtagacgtgg accttgacct tcagccgctg gagcagcccg ccgaagtaaa
     3361 gctggtcgcc gcccggcccc atcacgaagg ccccacccaa cggatgcacc cagacctcgc
     3421 tggcatgggc cgccagcagc atcccgtcgt cggtataggc catcgaatag gccagcaccg
     3481 gcttgccctt ggcgcgcacc ttgtcgagcg cgttgccgat ttcctgcagg gtcaccatgc
     3541 cgccgcccat gaattcggac aggtccagca ccaccgcctt gacccgctcg tcctcacccg
     3601 ccagcccgat ggcccggatc acgtcgcggg cgcggtgctc gcgcggttgg gcctggccgg
     3661 tcagcaggcc ttccagcgga tcgggcgtgg ccggttcttc caccaccgtc ccgttcaggc
     3721 gcagcacgag cgccccatcg cgcatcgcgg cggggctggg ccggctcatc agcacgccgt
     3781 agagcgccat gaagaacaac agcaggaaaa tcagaaccag accgtccttg atagcgacaa
     3841 gcaggttcca gaccttgcgg gcgaatgcca tgcgggttcc ttgtaagcgg tttgaaccgg
     3901 attaggcacc gcgcgcggga aaggcgagtc gaacgtcgat gagcggcaca ggcggatcgg
     3961 cgggtgacaa ccgccgcgct ccttgcccct tggcccttgc cccctgcccc gacgcggtct
     4021 agagcaccgt cttcaatgac atcgccgaac gcaaacagcg cgggccgcta tccagcaggc
     4081 tccgccgcct tcccgcaccg cgacctgacg gggctggcgg gactcgctcc ttgggaaatc
     4141 ctgttcctgc tcgacgaggc ggaacaatgg gtcgaactga accgccagcc gcacaagcgc
     4201 gacgaccgcc tggccggcct gaccatcatc aacgccttct tcgaaaactc cacccgcacg
     4261 ctgctgtcct tcgagatcgc cggcaagcgt ttgggggcgg acgtggtcaa catgctggtg
     4321 gcgcaatcga gcgtgaagaa gggggaaacc ctgatcgaca cggcgatgac tctgaacgcg
     4381 atgcacgccg atgccatggt gatccgccac cagagttcag gcgcggtgcg gctgatcgcc
     4441 gagaaggtcg attgcccggt gctcaacgcc ggtgacgggc agcacgagca tcccacccag
     4501 ggcctgctcg acgccctgac catccgcaag gccaagggcg atttcggcgg cctgcgggtc
     4561 acgatctgcg gcgatatcct gcacagccgg gtggcccgtt cgaacatcca ctgcctgacc
     4621 acgctgggcg ccgaggtgcg ggtctgcgcc ccgcccgcgc taatgcccgc cgggatcgag
     4681 gcgatgaagg tcaccccgtt ccacgatttc gacgcggcgc tgaaaggggc cgatgtcgtg
     4741 atgatgctgc gcttgcagca ggaacggatg agcgggcagt tcatccccag cccccgcgaa
     4801 taccggcatc tttacggcct gacgctggac cggctggcca aggccgcgcc ggacgcgctg
     4861 gtcatgcatc ccggcccgat gaaccgcggg gtggagatcg acagcaacgt ggccgatctg
     4921 gccgggcgct cggcgatcac cacccaggtc gaaatggggg tggcgatgcg gatggcctgc
     4981 ctggacgtac tgacccgcag ggcccgcggc gtggagggct gggcatgagc gcgccccgcc
     5041 ccctgaccat cgtcggcggc cagctggtcc tgcccggtac gcggcccgtc cccggcaccc
     5101 tgcgctgcgc cgatgggcgg atccaggcga ttggcggggc agagcccggc ccggacgatc
     5161 tggtgatcga cgcccgcggc gcgctggtcg cacccggtct ggtcgacctg ggcgtcttcg
     5221 cgatcgacaa gccggcctgc catttcggcg ggatcacccg cgcagccctg atgcccgacc
     5281 agggcgtggt gctcgacaag cccagcgcgg tccgctttgc cgcccagtcc ggcaagccgg
     5341 acctgtgggt ccacccgctg ggcgcggcga cccgcgacct ggccggaacc gaactggccg
     5401 aactggccct gatgcgcgat gccggggcca aggccgttgc caccggacgc cgctggattg
     5461 ccgacagcgg caccatgcac cggctgctgt cctattgcgc gatgctgggt ctggtcgtgg
     5521 tctcccacgc cgaggacggc ggcctggccg gatcggccgt ggccacggcc ggcgaaatgg
     5581 ccacccggct gggcctgccc agtgccccgg ccgaggccga ggcgctcgcc gttgcccgcg
     5641 acatcgcact ggccgaactg accggggccc ggctccattt ccgccaggtc accacccgtg
     5701 ccgcgttggc gctggtccgg gccgcccggg cccgcggcgt ggcggtcagc gccggggtaa
     5761 cgccggccca cttcatgctg tccgacgtgt cgatcggcga tttccgcacc ttcacccgga
     5821 tgtcgccgcc gctgcgcagc gaggacgacc gccaggccgt gcgcgaagcc ctggccgacg
     5881 ggacgatcag cgtgatcgca tcgggccacg atccgcgcgg acccgaagac aagcgtctgc
     5941 cctttgccga tgccgaacct ggcatggccg gggccgaaac cctgctggcg ctgaccctgg
     6001 ggctggtctg cgacggagtg atcggcacgg cccgcgcctt tgaactgctc tgcaccaacc
     6061 cggcgcggat cctgggggtc gaggcgggcc ggcttgaagt gggcgctgag gccgacatcg
     6121 cggtgatcga tcccgacaag ccgtgggtgg tcgattcgga caagatggcg gccgccgccg
     6181 gcaacacccc gttcgacaag cagggcgtcc agggccgggt cctggccctg ttcaagggcg
     6241 gcgttccggt cggctgaaac gcagaccccc tcccgcacgg ggcggaaggg ggctggcgtg
     6301 ggcttgtctg cccgtttgaa agcgaaactt acttcttgcg ggcgttgctc ggcccggccg
     6361 gagcggcctt gggcttggcc gccgcgacgg ccggagccgc cttcggcttg gcccgcgccg
     6421 ccatgaccgg cgcattggcc ggacggcccg gaaccgcgcg cacggtgg
//
LOCUS       DASUDV010000182         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1287182,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000182 DASUDV010000000
VERSION     DASUDV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287182"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1382)
                     /locus_tag="VFV30_03820"
     CDS             complement(54..1382)
                     /locus_tag="VFV30_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX4847246.1"
                     /translation="MTGRAIPMPTARALLLIAVAAPLAVLLAALAPALAATAPILGTL
                     VLVLIGIDAWLAGRLAAVDFAADEDVEVGEEGWMRIAARFAGGRRSGVECAIAADPRL
                     MPGGRGAARLDHLGGDDWKAALRFRPTRRGSGTIDRLWLRWTGPLGLGARQLRRDQAS
                     LVRVWPNLAPVRSPVLQAFLRNSEFGQIARRIRGEGTMFEALAEYQPGMDRRRIDWKA
                     SARHAHLYAKEFEAERNNQIVLAFDCGQAMCEPVAGLARIDRAVSAALAMAYVALKGG
                     DKVALYGFAARPEILTPFAAAARDFHRLQKAAAGLDYHAQEPNFTLALASLAGRLQRR
                     SLIVVFSDFSDPTSAELMVESIARLTSRHLVLFVTMEDEELGSLAQAPPETMEQLGMA
                     VSADALLRQRALVLSRLRQIGVDVIEAPHDRIGTRLIDAYLAIKGKGAIG"
     gene            complement(1387..2343)
                     /locus_tag="VFV30_03825"
     CDS             complement(1387..2343)
                     /locus_tag="VFV30_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4847247.1"
                     /translation="MTLAEVSALAGAIRAEVGKAVVGQAETLDGLLVALLAQGHVLLE
                     GPPGTAKTFLAQCFAASLGLDFGRIQFTPDLMPGDILGSNLFNFQTSQFTLTRGPVFC
                     ELLLADEINRTPPKTQAALLEAMQERRVTLDGTAHALPASFLVVATQNPIESQGVYPL
                     PEAQLDRFLFKLLVPYPSLAEEARIVATFGERAGPQRPADLGIGAVADAGVIAAAAQA
                     VKTVTLAESVVDYVVRLIRATRDSADLAAGASPRAAVLLANAARARAALDGRGYVVPD
                     DVKALAAPVLRHRLLLSPAAEIEGKAVEALVSDLVEATEAPR"
     gene            complement(2349..3668)
                     /locus_tag="VFV30_03830"
     CDS             complement(2349..3668)
                     /locus_tag="VFV30_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX4847248.1"
                     /translation="MNQAGDSGFSQRTVLALSLFGIAVFVALLWMIGSGAAESEPQPS
                     GAHVSGKGLSGYAALAGYLERRGFEVSRARTRGALKQRGVLVLTPLHSGGVDDIAAVV
                     NDHRMAGPTIVVLPKWIALEAPENVAKGRKGWVILADARPPMWPGFHDEIVLNLQAEQ
                     DSAWYGGPVDGELPDDKAVLSATIGKVPGKDDAWPHVPLVKADSGRMLASYVHDGNDY
                     PGLRNIALEDEPQFEEEVEGDGSYPLIFIYEPDLINNYGLARQENAWLAERLVMAALD
                     GDERKVTFDLTLAGPGRSENLLTLAFTAPFLAATVCLLLAAAVLMWRAMNRFGPPLAP
                     ARELAFGKHVLVANAAGLVRRAGRLHLIGGPYADASRERLARALALPARLDPEALEAA
                     IDRALAARRPQAEPYSAAAAAMRAARRPIDLLRAAQTLHSLERILTR"
     gene            complement(3665..>4362)
                     /locus_tag="VFV30_03835"
     CDS             complement(3665..>4362)
                     /locus_tag="VFV30_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847249.1"
                     /translation="LPCEQADVTAPAPVIQGSEVAQQAARDWQAVHADPAIQFAPFQI
                     PVVPPQPPGWLRWLLEGLQKLFEPLGKALGMSWPVMQWVLVALAALALGYALWRLSEP
                     LRERVPRGEAAGPDEWTPARAEALALLEDADRLAAEGHYAEATRLLLRRSVAQIAAAR
                     PDWVNPASTARELAALPALPERARGAFAAIALRVERAFFALRDLDAEDWQVARSAYAE
                     FALEQLPAAGQAA"
BASE COUNT          731 a         1534 c         1445 g          652 t
ORIGIN      
        1 gatcgcctgc tggcgatcgg ccggtgccgg tgcggtctgt ccgggaatag ccatcagccg
       61 attgctccct tgcccttgat tgccagatag gcatcgatca gccgggtgcc gatccggtca
      121 tgcggtgcct cgatcacgtc aaccccgatc tggcgcaggc gcgacagcac cagtgcgcgc
      181 tggcgcagca aggcatcggc gctcaccgcc atgccgagct gctccatcgt ttcgggcgga
      241 gcctgggcga gcgaacccag ttcctcatcc tccatggtca cgaacagcac cagatggcgc
      301 gaagtcagcc gggcgatgct ttcgaccatc agttcggcgc tggtcggatc ggagaaatcg
      361 gaaaagacca cgatcaggct gcgacgttgc agacgtccgg caaggctggc cagcgccagg
      421 gtgaagttgg gttcctgggc gtggtaatcg agccctgcgg cagctttctg caagcggtgg
      481 aaatcgcgcg cggcggcagc aaagggggtg aggatttcgg gccgggcggc aaaaccgtag
      541 agcgccacct tgtcgccccc cttcagcgcg acataggcca ttgccagcgc agccgaaacc
      601 gcacggtcga tccgcgccag cccggccacg ggttcgcaca tcgcctggcc gcagtcgaag
      661 gccaggacga tctggttgtt gcgttcggct tcgaattcct tggcataaag gtgggcatgg
      721 cgagcgctgg ccttccagtc gatccgccgc cggtccatgc ccggctgata ttcggccagg
      781 gcttcgaaca tcgtgccctc gccgcggatc cggcgcgcga tctgcccgaa ttcgctgttg
      841 cgcaggaaag cctgcaagac gggcgagcgt accggggcaa gattgggcca tacccgtacc
      901 aggctggcct gatcccggcg cagctgcctc gcgccaaggc ccagcgggcc ggtccagcgc
      961 agccacaacc ggtcgatcgt gccgctgccg cgccgggtag ggcgaaagcg cagcgctgcc
     1021 ttccagtcat ccccgcccag atggtcgagc cgagccgcgc cgcgaccgcc tggcatcaac
     1081 cggggatcgg cggcaatcgc gcattcgaca cccgagcgcc gcccgcctgc gaaccgcgcg
     1141 gcgatccgca tccagccttc ctcgcctacc tcgacatcct cgtccgccgc aaaatcgacc
     1201 gcagcgaggc gcccggcaag ccaggcatcg atcccgatca gcaccagcac cagggtgccc
     1261 aggatcggcg ccgttgccgc caaggccggg gccagcgctg ccagcagcac ggcaagcggc
     1321 gccgccaccg caatcagcag cagcgcgcgc gcagtgggca tcgggatggc gcgcccggtc
     1381 atcgcttcag cgcggcgcct cggtcgcttc gaccaggtcg ctaaccagcg cctcgaccgc
     1441 ctttccctcg atttcggcgg cgggtgacag cagcagccgg tgcctgagga ccggggcggc
     1501 cagcgccttg acatcgtcag gcacgacata gccgcgccca tccagcgcgg cgcgcgcccg
     1561 cgcggcattg gccagcagca ccgcggcgcg cgggctggcc ccggccgcca gatcggcgct
     1621 gtcgcgcgtg gcgcggatca gccgcaccac gtagtcgacc acgctttcgg caagggtcac
     1681 ggtcttcacc gcctgggccg cggcggcgat cacgccggca tctgccaccg cgccgatccc
     1741 cagatcggcc gggcgttgcg gtccggcccg ctcgccgaat gtggccacga tccgggcctc
     1801 ttcggccagg ctgggataag gcaccagcag cttgaacagg aaccggtcaa gctgggcttc
     1861 gggcagcgga tagaccccct ggctctcgat cgggttctgg gttgcgacca ccaggaaact
     1921 ggccggcagg gcatgagccg tgccatcgag cgtgacgcgc cgttcctgca tcgcctcgag
     1981 cagcgcggcc tgggtcttgg gcggggtgcg gttgatctcg tcggccagca gaagttcgca
     2041 gaagaccggc cccctggtaa gggtgaactg gctggtctgg aagttgaaca ggttcgaacc
     2101 caggatatcg cccggcatca gatcgggggt gaactggatc cgcccgaaat cgagcccgag
     2161 actggccgcg aagcactgcg ccagaaaggt cttggccgtt cccggcggac cttccagcag
     2221 cacgtgtccc tgggccagca aggccaccag caggccatcg agcgtttcgg cctggccaac
     2281 caccgccttg cccacttcgg cgcggatcgc gccggccagc gcgctcacct cggccagagt
     2341 catggttgtc atcgtgtcag gatcctttca agcgaatgga gtgtctgcgc cgcgcggagc
     2401 aagtcgatcg gccggcgcgc cgcgcgcatc gccgcagcag ctgcggaata gggttcggcc
     2461 tgcggccggc gtgccgccag cgcccggtcg atcgcagctt cgagcgcttc gggatcgagc
     2521 cgcgcgggca gggccagcgc ccgggccagg cgctcgcgcg aggcatcggc atagggcccg
     2581 ccaatcagat gcagccgccc tgcccggcgc accagccccg cggcattggc gaccagcacg
     2641 tgcttgccga aggccagttc gcgcgcaggc gcaagcggcg ggccgaaccg gttcatcgcc
     2701 ctccacatca gcacggccgc agccagcagc aggcaaaccg tcgccgccag aaacggcgca
     2761 gtgaaggcaa gcgtcagcag attctccgaa cggccagggc cggcaagggt gaggtcgaat
     2821 gtaaccttgc gctcatcgcc atcgagcgcc gccatcacca gtctttcggc cagccaggca
     2881 ttctcctgac gggcaaggcc atagttattg atgaggtccg gctcgtaaat gaagatcagc
     2941 ggataactgc catcgccttc gacttcttcc tcgaactggg gctcgtcctc gagcgcgatg
     3001 ttgcgcagcc ccggatagtc gttgccgtcg tggacatagg aagcgagcat ccggccgctg
     3061 tccgccttga ccagcgggac atgcggccag gcatcgtcct tgccgggcac cttgccgatc
     3121 gtggccgaaa gcaccgcctt gtcgtcgggc agttcgccgt caaccggccc gccataccag
     3181 gccgaatcct gctcggcctg caggttgagc acgatctcgt cgtggaagcc cggccacatg
     3241 ggcgggcggg catcggccag aatgacccag cccttgcggc ccttggcaac gttttccggt
     3301 gcttccagcg cgatccattt gggcaggacc acgatcgtcg gaccggccat gcggtggtcg
     3361 ttcaccactg ccgcgatatc gtccacaccg cccgaatgca gcggcgtcag caccagcacg
     3421 ccgcgctgct tgagcgcgcc gcgggtgcgg gcccggctga cttcaaaacc acgccgttca
     3481 agatatcccg ccagcgcggc atagccgctc agccccttgc ccgaaacgtg tgcgccgctg
     3541 ggctgcggct cgctttcggc cgccccgctg ccaatcatcc acagcagggc aacgaagact
     3601 gcgatgccga acaggctcag cgccagcacg gtgcgctggg aaaatccgct gtcgccggct
     3661 tggttcatgc cgcctgcccc gctgcgggaa gctgttcgag cgcgaattcg gcgtaggcgc
     3721 tgcgggccac ctgccagtct tcggcatcga gatcgcggag cgcgaagaaa gccctttcaa
     3781 cccgcagggc gatcgctgca aaggcaccgc gcgccctttc aggcagcgcg ggcaaagccg
     3841 caagttcgcg ggcagtgctg gccgggttga cccaatcggg ccgggccgcc gcgatctgcg
     3901 caaccgagcg ccgcaacaag aggcgggtgg cctcggcata gtgaccctcg gcggcaagac
     3961 gatcggcatc ctcaagcagg gccagggcct cagcgcgggc gggcgtccat tcgtcaggcc
     4021 cggccgcttc gcctctgggc acccgttcgc gcaagggttc gctcagccgc cagagcgcat
     4081 agcccagggc cagcgcggcg agcgccacca gcacccattg catcaccggc cacgacatgc
     4141 cgagcgcctt gcccagcggc tcgaacagct tctgcaggcc ctccagcaac cacctcagcc
     4201 agccgggggg ttgtggtggc acgaccggta tctggaacgg cgcgaactgg atcgcgggat
     4261 cggcatgcac agcctgccag tcgcgcgcgg cctgctgcgc aacttcggat ccctggatga
     4321 ctggcgcagg tgcggtcaca tctgcctgtt cgcacggcaa ta
//
LOCUS       DASUDV010000183         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1640522,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000183 DASUDV010000000
VERSION     DASUDV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640522"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFV30_03840"
     CDS             complement(<1..905)
                     /locus_tag="VFV30_03840"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX4847250.1"
                     /translation="MPRLDARSPMPDSLRGAILALGNFDGFHRGHQAVAGEAIAWARA
                     EGRPAIIATFDPHPVRYFAPDAPPFRLTTLDQRQELFEAAGADAMLVFAFDGELAGTT
                     AEAFVTALLAGRLGAAGVVTGEDFTFGKGRGGNVRVLAELGAASGMRARAVGPVSEDG
                     AVVSSSRIREALKGGDCGAATHLLTRPFAIRGVVEHGAKLGRTIGYPTANLRLGTYLR
                     PAYGIYAVTGVLPGGRVVHGAANLGIRPSFDPPIELLEPYFFDFSGDLYGQEIEVALH
                     HYLRPEAKFDSLEALTAQMEEDCKTA"
     gene            complement(908..2107)
                     /locus_tag="VFV30_03845"
     CDS             complement(908..2107)
                     /locus_tag="VFV30_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX4847251.1"
                     /translation="MRKRIWIPAAVLGLAAIGFFGIAPGWLEASMNRIDGKELIPVSP
                     EAEALHKSLLVVDLHSDTLMWKRDLTAPASRGHQDLPRLQKGNVALQVFSSVTKTPRG
                     QNYDANSDETDNITPLVIAQLQPRRTWTSLLERSLYHAEKLDAAVAASDGQLLKVTDE
                     ASFDRMLAAHGKAHGPVGALLSIEGLQNLEGRIENLDRLHAAGFRMAGMAHFFDNDVA
                     GSLHGVAKGGLTPFGRQVLGRMEELGMIVDIAHCSHQCVAEILATARRPVVSSHGGVQ
                     AVCKVNRNLSDEEIRGVARTGGVIGIGYWDAAICDTSPRSIAKAIKHVRDLVGIDHVG
                     LGSDYDGATTVRFDTAHLAQITQALLDEGFTVDEIRAVMGGNALRLIRAGIVPMSRQQ
                     LEQKRRS"
BASE COUNT          330 a          758 c          748 g          351 t
ORIGIN      
        1 gccgtcttgc agtcttcttc catctgggcc gtcaacgctt ccaggctgtc gaacttcgct
       61 tcgggccgca ggtaatggtg gagcgcaact tcgatctctt gtccatagag atcgccggaa
      121 aaatcaaaga agtagggttc cagcagctcg atcgggggat cgaagctggg gcggatgccc
      181 aggttggccg cgccgtgaac cacccgcccg ccgggcagga ccccggtcac ggcatagatc
      241 ccgtaggccg ggcgcagata ggtgcccagt cgcaggttgg cggtgggata gccgatcgtc
      301 cggcccagct tggccccgtg ctccaccacg ccgcggatcg cgaagggccg ggtcagcaga
      361 tgggtggccg cgccgcaatc gccgcctttc agcgcctcgc ggatccggct ggaagagacg
      421 accgcgccgt cctcgctgac cgggccgacc gcgcgggccc gcattcccga tgccgcgccc
      481 agctcggcca gcacccgcac gttgccgccg cggcccttgc cgaaagtgaa atcttcgcca
      541 gtgacgaccc cggccgcacc cagccggccg gccagcaggg cggtgacgaa ggcttcggcc
      601 gtggttccgg ccagctcgcc gtcgaaggcg aagaccagca tggcatcggc cccggccgcc
      661 tcgaacagct cctgccgctg gtccagcgtg gtcaggcgga acggcggggc atcgggggcg
      721 aaatagcgca ccgggtgggg atcgaaagtg gcgatgatcg ccgggcggcc ctcggcccgc
      781 gcccaggcaa tcgcctcgcc ggccacggcc tggtggccgc ggtgaaagcc gtcgaagttg
      841 cccagcgcca ggatggcgcc gcgcaagctg tcaggcatcg gggatcgtgc gtctaaccgc
      901 gggatggtca tgaccggcgc ttctgctcca gctgctggcg gctcatcggc acgatcccgg
      961 cccggatcag gcgcagcgcg ttgccgccca tgaccgcgcg gatctcgtcc accgtgaagc
     1021 cttcgtcgag cagcgcctgg gtgatctggg cgaggtgcgc ggtgtcaaac cgcacggtcg
     1081 tcgccccgtc atagtcgctg cccaggccga catggtcgat ccccaccagg tcgcgcacgt
     1141 gtttgatcgc cttggcgatc gagcgcgggc tggtgtcgca gatcgccgca tcccaatagc
     1201 cgatcccgat cacgccgccg gtgcgggcga cgccgcggat ctcttcgtcg gaaaggttgc
     1261 ggttgacctt gcagaccgcc tggaccccgc cgtggctgga gaccaccggg cgccgcgccg
     1321 tggcgaggat ctcggcgacg cactggtggc tgcaatgggc gatgtcgacg atcatgccga
     1381 gctcttccat ccgtcccagc acttgccgcc cgaacggtgt cagcccgccc ttggccacgc
     1441 cgtgcagcga tccggccacg tcgttgtcaa agaaatgggc catgccggcc atgcgaaagc
     1501 cggcggcgtg gagccggtcg agattttcga tccgcccctc gaggttctgc agcccctcga
     1561 tcgagagcag cgcgccgacc gggccgtggg ccttgccgtg ggccgcgagc atccggtcaa
     1621 agctggcctc gtcggtcact ttcagcagct ggccgtccga agcggcaacc gcggcgtcca
     1681 gcttctcggc gtggtagagc gagcgttcga gcagcgaggt ccaggtccgg cgcggctgca
     1741 gctgggcaat caccagcggg gtgatgttgt cggtctcgtc cgaattggca tcgtaattct
     1801 gcccgcgcgg ggtcttggtg accgaggaga agacctgcag cgccacgttg cccttttgca
     1861 gccggggcag gtcctggtgg ccgcggctgg ccggcgcggt caggtcgcgc ttccacatca
     1921 gggtgtcgga atggagatcg accaccagca ggctcttgtg cagggcctcg gcctcggggc
     1981 tgaccgggat cagctccttg ccgtcgatcc ggttcatgct cgcctcgagc cagcccggcg
     2041 cgatgccgaa aaacccgatt gccgccagcc ccagcacggc cgccggtatc cagatccgct
     2101 tgcgcatggt cacgccccct cctgcttcgt ccccggttta cgcagcagcg tgacgaaatc
     2161 aaaccccggc cgtcctgcct cggcggg
//
LOCUS       DASUDV010000184         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1375284,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000184 DASUDV010000000
VERSION     DASUDV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375284"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFV30_03850"
     CDS             <1..977
                     /locus_tag="VFV30_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847252.1"
                     /translation="MTSDRLRNLVAAASASPSVHNVQPARWRIDGAGLVLLEDRTRRL
                     AVGDPSGHDAAISLGAAAEGLVLAAGREGLVATVERLPDSGEAMPAIARFSFSPGTPS
                     DPLAAHLESRASWRGAFAPPEPADREAAAALTAEDRTVAASPEDIAWLAAQCDEASYG
                     FMRNGGFRAELRGWMRLSRGHPDWARDGLNAEAMRLSGVEALGAGLVLGPLFRPLAAL
                     GLAPHLLAERKSFDAAAGVALFHRPSGEDPFESGRHFHRCWLALEAAGFGANVLAALA
                     DDKAIAARIAERFGIGPQRRLVSAFRFGRRSGSGFPRARLPLSEVLLS"
     gene            complement(974..2608)
                     /locus_tag="VFV30_03855"
     CDS             complement(974..2608)
                     /locus_tag="VFV30_03855"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4847253.1"
                     /translation="MSDASALPDRPPKRRAVWGLLGLTLAFAGYAVLGAERNAASVVL
                     PPVDPSAPAEQRGAAAFLGAGFGGISQRSLESNAIPWKLAAAALVLDEQSRDPQAAAD
                     QATLRRVLARFGFLFPERLLGVPEGLTPAFAAKPLGMTHGTIAPLGGAKVEVANLGCA
                     ACHSGVGYDAAGNPVTAEAVPGMPNTSLDLESYTQAIFLAMRRFGNDDALLATAERLF
                     PDMDWREKASLRWLVLPLAKSRLAELDGQDRAMPFPNGLPGATNGVAALKAQFGTPLR
                     DGGALDRGFVSIPELALRDRRSRLLTDGAYAAPGGRADPKALAAITSFFTVPSMGVHP
                     DEARSHQGEAEAIFAWLAQYRPPPFPGPVNLEQARRGEAIYARDCAACHGAIRWDSAR
                     PELARYTDWVGNVGTDPLRADLFDAPLAKAVGDSGYGDAITVKTGQGYAAPPLAGLWA
                     SAPYLHNGSVQSLEALLNPARRLPRFMVGGHALDWNTVGIRLGPDGRYPAGYVPFATP
                     HWVDTALPGLGNGGHTFGQDLTEADRRALIAFLLLL"
     gene            complement(2723..2812)
                     /locus_tag="VFV30_03860"
     tRNA            complement(2723..2812)
                     /locus_tag="VFV30_03860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2776..2778),aa:Ser,seq:tga)
     gene            complement(2870..3277)
                     /locus_tag="VFV30_03865"
     CDS             complement(2870..3277)
                     /locus_tag="VFV30_03865"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEX4847254.1"
                     /translation="MVRKLLELGAPLAVLAFMALPLKAQPVEATANWKVVAVDSEEGE
                     TEHIDTTSIERDGGIVTFWQKTEFSRDPQGWKTSYLKLEFDCPARKLRMMDLVVVYTD
                     GTKSDEEGSIDKWFPIPADSLAAKIYPHVCPAK"
BASE COUNT          527 a         1215 c         1229 g          559 t
ORIGIN      
        1 gcatgacctc tgatcggctc cggaaccttg tcgccgcggc cagcgcatcg cccagcgtcc
       61 acaacgtcca gccggcgcgc tggcggatcg acggggccgg gctggtgctg ctggaggacc
      121 gcacccggcg cttggccgtg ggcgacccga gcggacacga cgcggcgatc agcctggggg
      181 cagccgcgga ggggctggtt ctggcggccg ggcgcgaagg actggtcgcg acggtagagc
      241 ggctgccgga cagcggcgag gcgatgccgg ccatcgcccg gttcagcttt tcgccgggaa
      301 cgccgagcga tccgctcgcc gcgcatctgg aaagccgggc ctcatggcgc ggagccttcg
      361 cgccgcctga gccggccgat cgggaggcag ctgccgcctt gaccgcggag gataggaccg
      421 ttgcggcaag ccccgaagac atcgcctggc tggccgcgca gtgcgacgag gccagctatg
      481 gcttcatgcg gaacggcggc tttcgcgccg agctgcgcgg ctggatgcgg ctgagccggg
      541 gccacccgga ttgggcccgc gacgggctca acgccgaggc catgcgcctg tcgggcgtcg
      601 aggccctggg ggcagggctg gtactgggcc cgctgttccg cccgctggcg gcgctgggcc
      661 tggcaccgca cctgctggcc gagcgcaagt ccttcgatgc cgccgccgga gtagccctgt
      721 tccaccgccc gtcgggcgag gatccgttcg agagcggacg gcatttccac cgctgctggc
      781 tggcgctcga ggctgccggg ttcggagcca atgtcctggc cgcgctggcc gatgacaagg
      841 ccattgccgc gcgcattgcc gaacggttcg ggatcggtcc gcagcggcgt ctggtcagcg
      901 ccttccggtt cgggcggcgc agcggttcgg gctttccccg cgcccgtctg ccgctcagcg
      961 aagtgttgct tagctaaagc aacaacagga acgcgatcag cgcgcgccgg tcggcttccg
     1021 tcaggtcctg cccgaaggtg tggccgccgt ttccaagccc gggcagcgcg gtatcgaccc
     1081 agtgcggtgt cgcaaagggc acatagcccg ccggatagcg cccatccggc cccagccgga
     1141 tgccaaccgt gttccaatcc agcgcgtgcc cgccgaccat gaatcgcggc aggcgcctgg
     1201 ccgggttcag cagcgcctcc aggctctgca ccgacccgtt gtgaaggtag ggcgccgagg
     1261 cccacagccc ggctagcggc ggcgcggcat agccctggcc ggtcttgacc gtgattgcat
     1321 cgccatagcc gctatcgccc accgccttgg ccagcggcgc atcgaacagg tccgcgcgga
     1381 gcggatcggt gccgacgttg ccgacccagt cggtgtagcg cgccagttcg ggccgcgcgc
     1441 tatcccagcg gatcgcaccg tggcacgccg cgcaatcgcg ggcgtagatg gcctcgcccc
     1501 ttcgggcttg ctccaggttg accggaccgg ggaagggcgg cgggcggtac tgcgccagcc
     1561 aggcgaagat cgcctcggcc tcgccctggt gggatcgggc ttcatcggga tggaccccca
     1621 tgctgggcac ggtgaagaag ctggtgatcg cggccagcgc cttcggatcg gcgcgcccgc
     1681 cgggggccgc atacgccccg tcggtcagca ggcggctgcg ccggtcgcgc agcgccagtt
     1741 ccggaatgga gacgaagccc cggtccagcg cgccgccatc gcgcagcggt gtaccgaact
     1801 gcgccttcag cgccgcaaca ccgttggtcg cgccgggcag tccgttgggg aaaggcatgg
     1861 cccggtcctg cccgtccagc tcggccaggc ggctcttggc cagcggcagg accagccagc
     1921 gcagactggc cttttcgcgc cagtccatgt ccggaaacag ccgttcggcc gtggccagca
     1981 aggcatcgtc gttgccgaag cggcgcatcg ccaggaagat cgcctgggtg tagctttcca
     2041 gatcgagcga ggtgttgggc attcccggca cggcttcggc cgtgacggga ttgcccgccg
     2101 catcgtagcc gacgccggag tgacaggcag cgcagcccag gttcgcgacc tcaaccttgg
     2161 ccccgcccag cggcgctatg gtgccgtggg tcatgcccag cggcttggct gcaaaggcgg
     2221 gagtcagccc ctcgggcacg ccaagcagcc gctccggaaa caggaagccg aaccgggcca
     2281 gaacccggcg cagggtggcc tgatcggcgg cggcctgcgg atcgcggctc tgctcatcca
     2341 gcaccagcgc cgcggcggcg agcttccagg gaatcgcgtt gctttccagc gagcgctggg
     2401 aaatgccgcc gaacccggcg ccgaggaaag ccgcggcgcc ccgctgttca gccggggccg
     2461 atggatccac cggcggcaac accaccgagg ccgcgttgcg ttcggctccc agcacggcat
     2521 agccggcaaa ggccagggtc aggcccagca agccccagac cgcgcggcgc tttggtggtc
     2581 tgtcaggcaa tgcggatgcg tcgctcaatg aagatccccg tccggctgaa gtgcccttcc
     2641 aggggaacat aggcaggccc cgcgcaaccg caaaccgttt cgtgtgcggg gcttaacgcc
     2701 ttgcggcaac agggatcagg ggtggcggac agggtgggat tcgaacccac ggtgagcttg
     2761 cacccacggc ggttttcaag accgctgcct taaaccactc ggccacctgt ccccgtgctt
     2821 cgcacgcgat gcgccgctag ccgttttgcg cggcgctgtc accccggtgc tatttggccg
     2881 ggcaaacgtg ggggtagatt ttcgcagcca ggctgtctgc tggaattggg aaccatttat
     2941 cgatgctgcc ttcttcatcc gacttggtgc cgtcggtgta gaccaccacc aggtccatca
     3001 tccgcaactt gcgggccggg caatcgaatt ccagtttcag ataggatgtc ttccaccctt
     3061 ggggatcgcg cgaaaactcg gtcttctgcc agaaggtgac tatgccacca tcgcgctcga
     3121 tcgaggtcgt gtcgatgtgc tcggtctcgc cctcctcgga atcgacggcc accactttcc
     3181 aattggccgt ggcttcgacc ggctgggcct tgagcggcag ggccatgaac gccagcacgg
     3241 caagcggcgc ccccagttcg agcaatttgc gcaccatgac cgtctcccta tcccgtccct
     3301 gcaacgcatt ggcgctttgc cgacacgggt caagcgtttg cccacgcgcg gcttttgccg
     3361 aatcgccgga cctgtgcaaa ggatgaccgc atgacgttgc tgcgcacctt acgcctttca
     3421 gccccgctgg cgctgtccgt cgcgctgctg tctgggccga atggcggagc ggaggcgcag
     3481 gtgacggcgc cggttgccga gcaggccgat ccggctgccg gttttcccgg
//
LOCUS       DASUDV010000185        11015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1264765,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000185 DASUDV010000000
VERSION     DASUDV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264765"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1171)
                     /locus_tag="VFV30_03870"
     CDS             complement(179..1171)
                     /locus_tag="VFV30_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4847255.1"
                     /translation="MKTRTLGRSGLAVSPLGLGCMGMSWAYGAAPDKAAMIALLRAAV
                     ERGVTFFDTAEVYGPFTNEELLGEALEPLRDRVVIATKFGFDLTRDPASGPGTNSRPD
                     HIRAVVDASLKRLRTDRIDLLYQHRVDPDVPIEDVAGTVGELIAAGKVSHFGLSEAGT
                     ETIRRAHAVQPVTALQSEYSLWRRDPERDVLPLLEELGIGFVPYSPLGRGFLTGAMSA
                     DTVFEKGDFRARLPRFQQEAMAANQALVEVLRGIAAPLGATVAQIALAWLLAQRDWIV
                     PIPGTTKLARLEENLGAVDLALSAADLAAIDAAARTMEIVGERYLPAMEAMTGR"
     gene            complement(1189..3171)
                     /locus_tag="VFV30_03875"
     CDS             complement(1189..3171)
                     /locus_tag="VFV30_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX4847256.1"
                     /translation="MPRPAPRLFAAALLLSSTAVLAQTAAAPADPALQPKPASASTLA
                     AQRAAAQTLPAEDGRDAEFAKRGFLGTRADPLIRNKQGRPVWNLAAYDFVTGPAPATV
                     NPSLWREMSFLRLHGLFEVTDGIYQVRGFDVSNMTVVRGATGWIVIDPLTTRETAAAA
                     MELVNAKLGPRPVVAVIYSHSHGDHYGGVRGVVSEADVKAGKVAIIAPEHFMKETASE
                     SVMAGASMGRRATYQFGTALQPGPQGQMGSGIGPGLAAGEITLIAPTDTITRTGETRT
                     VDGVDLEFQIVSGSEAPSELNVYIVPKRIFLSAEMSTCSLHNILTPRGAKVRDTHAWA
                     GYLDEALRLYGGRSDAVISSHCWPRFGQAESAAMLASQRDNYRYLHDQSVRRMNQGMT
                     QSEIAEGLVQPPELSQQWYNRGYYGTYSHNSKAIYQFYLGWYDGNPANLNPWPPVERA
                     KRYVAAMGGPKKVLAEARKAMAAGDYRWSSELLSQLVFADPTNLQARALLADSFEQQG
                     YQAESAIWRNQFLSAAKELRQGYKPQPIQAQNVDMISAVPTLLLLDTVATRYDPSRLS
                     REPFTVKFVLTDSKEVAGIEVGKTVLIGRMGEPASRPAVTITGPRQAMLALLFLRMPL
                     AQVQALGVKVEGDPSAVAALQAALDPLPEGFNIAEP"
     gene            3428..3973
                     /locus_tag="VFV30_03880"
     CDS             3428..3973
                     /locus_tag="VFV30_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX4847257.1"
                     /translation="MDSNPPSNLPPAPFIVTGELPGDVLAWSDGLRRAHFPPERNHLA
                     AHVTLFHSFAPSLREELPRMLARLAGEFAPPPAEVTGLIDLGQGTAIAIRSPALLAVR
                     GLLADHFWDMLTKQDQGGKKLHITVQNKVTRAQALALQAELGPALEHRRFAFTGLGLH
                     LYRGPHWEPVGVWAFRGKVSA"
     gene            4017..4093
                     /locus_tag="VFV30_03885"
     tRNA            4017..4093
                     /locus_tag="VFV30_03885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4051..4053,aa:Met,seq:cat)
     gene            complement(4105..5499)
                     /locus_tag="VFV30_03890"
     CDS             complement(4105..5499)
                     /locus_tag="VFV30_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX4847258.1"
                     /translation="MTQAAEGRFEGHPKGLYYLSFTEMWERFSFYGMSALLTLYMVKE
                     LLLPENAAQVIGLAPLRGVLASMMGDLSNVALASLIYGLYAGLVYFTPIVGGWVADRI
                     LGAKRTVMIGVLLMSAGHLAMSFYSTFLLALLLLILGSGFLKGNISAQVGMLYPREAE
                     SLRTRGFTIFSTGICIGAASGPIITGIAMAAYGFHAGFAVAAALMLVALIAYVLGQKH
                     LPDDRPVARERTKTPPLTPAERRRVLILLLVVAMTIPAEIAYPMVWGIGIVWVDQYVN
                     LSTPLGTMPSTWFASMDSIGAILAAPLLVSLWAWQARRGVEPSSVTKIGIGTGIVAIG
                     ALMFAYGNLGTTAPDSVNVGWALGGWLLMGLAWMYYWPTTLALVSRCAPSAIASTLMG
                     VAFLSPFIAHTMMGWVGTYFDKMSPSAFWAMDAGIAVAGAALLLALRGPLARALEPAE
                     GERREGEGTGPIHH"
     gene            complement(5546..6751)
                     /locus_tag="VFV30_03895"
     CDS             complement(5546..6751)
                     /locus_tag="VFV30_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847259.1"
                     /translation="MTSRLRGRTDLSNQTTAAATHAARPFTFTLIGIGAATAAAHIGN
                     NFTTYLVGGLIDRYAFTPFAMGAWNMAETLSYAAAMFLIAPRARSMDARTLALLASVL
                     IAVSQAGSALTGNYALLLLGRIGTGLGFGIMNSAVNLAAGRTDHPARWISLGIAFQTV
                     LYALVAIGLPKVGESQGVTGMFLALGALSVVLGAMAMLLPGGSGQTGEGTALPAPAAI
                     GPEGRRILAAMALFAFGSLAIWPFIERTAHAIGIPATQFGLIQSAAVLASALGNAALA
                     LVINRLPRGWPLAAALGACGLACALLTTVGSTFAFTAAFIGYMVSWFLTYPLLLGLTY
                     AHDDSGRLAVMTTGTWLLAQSIGSLVAGYIAQNFGGYTPIGPLGALFCLGAIAVALPV
                     ARRLDRQGV"
     gene            6963..8072
                     /locus_tag="VFV30_03900"
     CDS             6963..8072
                     /locus_tag="VFV30_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4847260.1"
                     /translation="MTVAALEAGADRLVVTWGDGAATSYPTIWLRDNCPSGLHPQTHE
                     RLLDLLSLDPAPRLLSAAQEGDAVRLDYADGHVSRMPIALLDAHRPGRPEADPAVVAP
                     EYWRADLTVAGIPRFSGPQIVADDAVLDAWMRQTARYGLSIVDGLGDQSDAGVAVAQR
                     IGFLRQTNFGTTFEVVSMPDPNNLAYTPVALPLHTDLPNQEVPPGFQFLHCLANEAEG
                     GGSIFADGFAIASDLRGRDPEAFRILSEVAIPYRFHDADYDIRVREPVITLDRAGELV
                     EIRYNAHIAAMFDMPADLMPAFYRAYRAYMAMTRDPQYRLSFKLESGEMIVFDNRRVL
                     HGREAFDPSTGYRHLHGCYVDRGEFLSRLRVLARA"
     gene            complement(8069..8848)
                     /locus_tag="VFV30_03905"
     CDS             complement(8069..8848)
                     /locus_tag="VFV30_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4847261.1"
                     /translation="MNAVKLERHGEVLVITLDRPKANAIDVATSLELYAAFRELDEDP
                     ALRVGIITGAGRFFSAGWDLNAANEGEAADADHGPGGFAGLTEYFTLSKPVIAAVNGV
                     AVGGGFELALAADLIVASTAARFWLPEVQLGILPDSGGLLRLPKLLPPQLAREMILTG
                     RRLDAAEAAALGLASRLCEPEALLDTALELAREIAKGAPLAIKAARDILRATEGLEVE
                     RAYALMRSGAIPSYRAMLDSADALEGPLAFAEGRPPHWQGR"
     gene            complement(8845..10941)
                     /locus_tag="VFV30_03910"
     CDS             complement(8845..10941)
                     /locus_tag="VFV30_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX4847262.1"
                     /translation="MSPAKPLDRLLRPRSIAFVGGKAAEEAVRQSRAFGFDGAIWPVN
                     PNRADMAGVPCLPALVDLPDAPDAVFLAAPAGPTIALAGDLAAMGAGGAICYASGFAE
                     TGPEGQQRQAELLDAAGAMPLVGPNCYGMLNAFDRVALWPDQHGCLPIAEGRGVAIIS
                     QSGNLALNFTMQARGLPIGYVISVGNCADVTPADLIEALVRDERVSAIGLHLEGLGSV
                     ARFSEACLAARAAGVPLVALKTGASAKGAALTLSHTSSLAGADALCDALFARYGVARA
                     HDPAQFLETLKLLHVAGPLAGRRITSASCSGGEASLTADLAERAGLETPDFPPASQAE
                     LEEVLGPQVHVANPLDYHTYIWGDGPAQTRCFAGLIGAGFEAAILILDFPRADRCDAR
                     DWQTTLDAFVGAHRANPPQPGTVRIVASSLAENLPEAVCAQLLAAGLVPAAGLAEALA
                     AIAAAAGIGEVWSRPPAPALPEPLTALSGPAAVWDEVRSKAALARAGVPVPAGRTVTL
                     AEIEALCADPASPLPAPFPLVLKAVGEHLAHKTELGAVALGITSREGLLAEAQRMAPL
                     GGTFLIEEMVGGAVAELIVGVARDPQFGLFLTLGAGGIFVELLRQVEQVLLPASREEI
                     AAALARLPIDAVLQGYRGKPGCDRDALVNAITAIAAFALDHADRLEELDVNPLLALPR
                     GATAVDALIRMRETPE"
BASE COUNT         1718 a         3790 c         3827 g         1680 t
ORIGIN      
        1 ctttcgcccg atgtcgaaac cgccagccgg gttggagcgc ggcgcggcaa gccggtgatc
       61 ctgcggatcg cggcgcagga catggcgcgg ggcggggcgc agttccggct ctccagcaat
      121 ggagtctggc tggccgaggc ggtgccgccg gccttcatta ccgtcacctg agcgccgctc
      181 agcgcccggt catcgcctcc atcgccggga gatagcgttc gccgacgatt tccatcgtgc
      241 gggccgcggc gtcgatggcg gccagatcgg cggcgctcag cgccagatcg accgcgccga
      301 ggttctcctc cagccgggcc agcttggtcg tgccggggat cggcacgatc cagtcccgct
      361 gggccagcag ccaagccagc gctatctggg caaccgttgc gcccagcggt gcggcaatcc
      421 cgcgcagcac ctccaccagc gcctggttgg cggccatcgc ctcttgctga aagcgcggca
      481 ggcgcgcgcg gaaatcgcct ttctcgaaaa cggtgtcggc gctcatcgcc ccggtcagga
      541 agccgcggcc gagtgggctg taggggacaa agccgattcc cagctcctcc agcagcggca
      601 ggacatcgcg ttccggatcg cgccgccaga gcgagtattc gctttgcagg gcggtgaccg
      661 gctgaaccgc gtgggcgcgg cggatcgttt ccgttccggc ttcggacagg ccgaaatggg
      721 aaaccttgcc ggccgcgatc agctcgccca ccgtgccggc cacgtcctcg atcggcacat
      781 ccggatcaac gcggtgctgg taaagcaggt cgatccggtc ggtccgcaag cgcttcagcg
      841 aggcatcgac aaccgcgcgg atgtggtccg ggcggctgtt ggtgcccggg ccgctggcgg
      901 gatcgcgggt caggtcgaat ccgaacttgg tggcgatcac cacccggtcc cgcagcggct
      961 ccagcgcttc gcccagcagc tcctcgttgg tgaaggggcc atagacctcg gccgtgtcga
     1021 agaaggtcac gccgcgctcc accgcggcgc gcagcagggc gatcatcgcg gccttgtcgg
     1081 gcgcggcgcc ataggcccag ctcatgccca tgcagcccag ccccagcggg gatacggcca
     1141 ggcccgatcg gcccagggtg cgcgtcttca tggcagtctc cagttcgatc agggttcggc
     1201 gatgttgaag ccttcgggca gcggatcgag cgcggcctgc agcgcggcca ccgcgctggg
     1261 atcgccttcc accttgaccc ccaaggcctg gacctgggcc agcggcatcc gcaggaacag
     1321 cagggccagc atggcctggc gcggcccggt gatcgtcacc gccgggcgcg aggccggctc
     1381 gcccatccgg ccgatcagca ccgtcttgcc cacttcgatc ccggccactt ccttgctgtc
     1441 ggtcaggacg aacttgaccg tgaagggttc gcgcgaaagg cggctgggat catagcgggt
     1501 ggcgacggta tcgagcagca gcagggtcgg cacggccgag atcatgtcga cgttctgcgc
     1561 ctggatcggc tgcggcttgt acccctgacg cagctccttc gcggcggaga ggaactggtt
     1621 gcgccagatc gcgctttcgg cctgatagcc ctgctgttca aagctgtcgg ccagcagggc
     1681 gcgggcctgc aggttggtcg gatcggcgaa gaccagctgg ctcagcagct ccgacgacca
     1741 gcggtagtcg cccgccgcca tcgccttgcg cgcttcggcc agcaccttct tcggcccgcc
     1801 catcgccgcg acatagcgct tggcccgctc caccggcggc caggggttga ggttcgccgg
     1861 gttgccatcg taccagccca ggtagaactg gtagatcgcc ttcgaattgt ggctgtaggt
     1921 gccgtaatag ccgcggttgt accactgctg cgaaagctcc ggcggctgga ccagcccttc
     1981 ggcgatctcg ctctgggtca tgccctggtt catgcgccgg accgactggt cgtgcagata
     2041 gcggtagttg tcacgctggc tggccagcat cgcggcggat tcggcctggc cgaagcgcgg
     2101 ccagcagtgg ctggaaatca ccgcgtcgct gcgcccgcca tagagccgca gcgcctcgtc
     2161 cagatagccg gcccaggcgt gggtatcgcg caccttggcc ccgcgcgggg tcaggatgtt
     2221 gtggagcgag caggtgctca tttcggccga caggaagatc cgctttggca cgatgtagac
     2281 gttgagctcg ctgggcgcct cgctaccgga gacgatctgg aattccaggt ccacgccgtc
     2341 gaccgttcgg gtttcgccgg tgcgggtgat cgtgtcggtc ggcgcgatca gggtgatctc
     2401 acccgcggcg agccccgggc cgatccccga tcccatctgc ccttgcggac cgggctgcag
     2461 cgccgtgccg aactggtagg tcgcgcgccg gcccatcgag gcgccggcca tgacgctttc
     2521 cgaggcggtt tccttcatga agtgctcggg cgcgatgatc gccaccttgc cggccttcac
     2581 gtcggcctcg ctcaccacgc cgcgcacgcc gccgtaatgg tcgccgtggc tgtggctgta
     2641 gatcaccgcc acaaccgggc gcgggcccag cttggcattg accagctcca tcgctgcggc
     2701 ggcggtttcc cgggtggtca gcgggtcgat cacgatccag ccggtcgcac cgcgcaccac
     2761 ggtcatgttg gacacgtcga acccgcggac ctggtagatc ccgtcggtca cttcgaacag
     2821 gccgtggagg cgcaggaagc tcatctcgcg ccacagcgag gggttgaccg tggccggggc
     2881 ggggccggtg acgaaatcat aggccgccag gttccagacc ggccggccct gcttgttgcg
     2941 gatcagcgga tcggcgcggg tgcccaggaa gccgcgcttg gcaaactcgg catcgcgccc
     3001 gtcttcggcc ggcagggtct gggccgcggc gcgctgggcg gcgagggtcg acgcgctggc
     3061 gggcttgggc tgcagggccg gatcggcggg cgcggccgcg gtctgggcca gaacggcggt
     3121 gctgctcagc aacagggcgg cggcaaacag ccgcggggcg gggcggggca ttggcgggtc
     3181 tcctctctcc gccgcgcagg atgcggggga cggggacggc gcgcaagagg gttcaaccgg
     3241 gcgattaagc gggatcggtg ataagtgtga gaggatttgg cggtttctcc aatttgtcgg
     3301 cgcgagttag agcgaacggg cgatgaagcg acgcgggtgc cggatcctct cccccctgaa
     3361 cggatccggg tcctcgcgat cgaagccgcc ggtggccttc gggccggtaa ccggctaggt
     3421 gcgggcggtg gactccaacc ccccctccaa ccttccgccc gcacccttca tcgtgaccgg
     3481 cgaactgccg ggggatgtcc tggcttggtc cgacggcctg cgccgcgccc atttcccgcc
     3541 cgaacgcaac cacctggccg cccacgtcac cctgttccat tccttcgcgc cctcgctgcg
     3601 tgaggagctg ccgcggatgc tggcgcggct ggcgggcgaa ttcgcccctc cgccggcgga
     3661 agtcaccggg ctgattgacc tggggcaggg cacggccatc gccatccgca gcccggcgct
     3721 gctggcggtg cgcggtctct tggccgatca tttctgggac atgctgacca agcaggacca
     3781 gggcggcaag aagctgcaca tcacggtcca gaacaaggtc acccgggcac aggccctggc
     3841 cttgcaggca gagctggggc cggcgctgga acaccgccgc ttcgccttca cgggccttgg
     3901 cctgcacctc taccgcgggc cccattggga gccggtgggg gtatgggcgt ttcgcggcaa
     3961 agtgagcgct tgaccggggc gaaactgcac cctatgtgcg cgccctgcct tggtgaggcg
     4021 gagtagctca gccggttaga gcagcggaat cataatccgc gtgtcggggg ttcgagtccc
     4081 tcctccgcta ccaggtttcc cccgtcagtg atggatcgga ccggtgcctt cgccctcgcg
     4141 gcgttcgccc tcggccggtt ccagcgcgcg cgccagcggg ccgcgcagcg cgaggagcag
     4201 cgccgccccg gccacggcaa tgccggcgtc catcgcccag aacgccgagg ggctcatctt
     4261 gtcgaaataa gtgccgaccc agcccatcat cgtgtgggcg atgaacggtg agaggaaggc
     4321 gacgcccatc agggtggagg cgatcgccga tggggcgcag cggctgacca gcgccagggt
     4381 ggtgggccag taatacatcc aggccagccc catcagcagc caccctccca gcgcccagcc
     4441 gacgttgacg ctgtccggcg cggtcgttcc caggttgcca taggcgaaca tcagcgcgcc
     4501 gatcgcgacg atccccgtgc cgatcccgat cttggtgacg ctgctcggct ccaccccgcg
     4561 ccgggcctgc caggcccaca gcgagaccag cagtggcgcg gccaggatcg cgccgatcga
     4621 atccattgag gcgaaccagg tggagggcat ggtacccagc ggggtggaga gattgacgta
     4681 ctggtcgacc cagacgatcc cgatgcccca gaccatcgga taggcgattt cggccgggat
     4741 ggtcatggcg acaaccagca gcaggatcag cacccggcgc cgctcggccg gggtgagggg
     4801 cggggtcttg gtccgctcgc gcgccaccgg ccggtcatcg ggcaggtgct tctggcccag
     4861 cacataggcg atcagcgcca ccagcatcag cgccgcggcg acggcaaagc cggcgtggaa
     4921 gccgtaggcg gccatggcga tcccggtaat gatcgggccg cttgccgcgc cgatgcagat
     4981 cccggtcgaa aagatcgtga agccgcgggt gcgcagcgat tccgcctcgc gcggatagag
     5041 catgccgacc tgggcggaga tattgccctt gagaaagccc gagccgagga tcagcagcag
     5101 cagggcgagc aggaaggtcg aatagaacga catggcaagg tgcccagcac tcatcagcag
     5161 gaccccgatc atcaccgtgc gcttggcgcc caggatccgg tcggccaccc agccgccgac
     5221 aatcggggtg aaatagacga ggcccgcgta aagcccgtag atcagcgagg cgagcgccac
     5281 gttgctgaga tcgcccatca tggacgccag caccccgcgc agcggggcca ggccgatcac
     5341 ctgcgcggcg ttttccggca gcagcagctc cttgaccatg tagagcgtca agagcgccga
     5401 catgccgtag aacgagaacc gctcccacat ctctgtgaac gagagatagt acagcccctt
     5461 ggggtgccct tcgaaccggc cttctgccgc ctgcgtcatg gggatcctcg ccttcctgtg
     5521 cgtgtctgtt ttgcggggtg tgccgtcaaa cgccttgccg gtccagcctg cgcgcaaccg
     5581 gcagggccac ggcgatcgcg ccaaggcaga acagggcgcc cagcgggccg atcggggtgt
     5641 agccgccaaa gttctgggca atgtaccctg cgaccagcga accgatgctc tgcgccagca
     5701 gccaggttcc ggtggtcatc accgccagcc ggccgctgtc gtcgtgggca taggtcaggc
     5761 cgagcagcag cggataggtc aggaaccagc tgaccatgta gccgatgaag gcggcggtga
     5821 aggcaaaggt gctccccacc gtggtaagca gcgcgcaggc cagtccgcag gcgcccagcg
     5881 ccgccgccag cggccatccg cgcggcaggc ggttgatcac cagcgccagg gcggcgttgc
     5941 ccagcgcgct ggccagcacc gccgcgctct ggatcaggcc gaactgcgtc gccggaatgc
     6001 cgatggcatg ggcggtgcgc tcaatgaacg gccagatcgc cagcgagccg aaggcgaaca
     6061 gtgccatcgc cgccaggatc cggcggcctt cggggccgat cgcggccggg gcgggcaggg
     6121 cggtgccttc gccggtctgg cccgatccgc cgggcagcag catggccatc gcccccagca
     6181 cgaccgaaag cgcccccagc gccaggaaca tgcccgttac gccctggctt tcgcccacct
     6241 tgggcaggcc aatggcgacg agcgcataga gcacggtctg gaacgcgatc cccagcgaga
     6301 tccaccgcgc cggatgatcg gtccgtcccg cggcgaggtt caccgcgctg ttcatgatgc
     6361 cgaagccaag gccggtcccg atccggccga ggaggagcag ggcatagttg ccggtcaggg
     6421 ccgatccggc ctgcgacacg gcgatcagca cgctggccag cagagccagc gtccgcgcgt
     6481 ccatcgaccg ggcccgcggt gcgatcagga acatcgccgc cgcatagctc agcgtttcgg
     6541 ccatgttcca ggcccccatc gcgaagggcg tgaacgcgta gcggtcgatc aggccgccca
     6601 ccagataggt ggtgaagttg ttgccgatgt gcgctgcggc cgttgccgcg ccgattccga
     6661 tcagcgtgaa ggtgaaagga cgggctgcgt gcgtggcggc cgctgtggtc tggtttgaca
     6721 ggtcggttct cccccgcagc cgggatgtca tgtttccgga gcgggcgcaa cgcggcctcg
     6781 ctgcccttgc cgccggccgt tttccgatct agcctctgcc catggacccc gaatgcaccg
     6841 atttcgggct tggccggcgc ggttctggcg cgccttacgg caagacccaa gaatggtggc
     6901 cgccgccgcg cggctagccg ccattcgtac aatcgatacc ctgcattaga actggaaatt
     6961 ccatgactgt agctgcactc gaagccggtg cagaccggct tgtcgtgacc tggggcgatg
     7021 gcgcggccac ctcctatccg acgatctggc tgcgcgacaa ttgcccgtcc ggactgcacc
     7081 cgcaaaccca cgaacgcctg ctggatctgc tctcgctcga tcctgcgccg cgcttgctga
     7141 gcgccgcgca agagggagac gcggtgcggc tggactatgc cgacggtcac gtcagccgca
     7201 tgccgatcgc cttgctcgat gcccaccgtc cgggccggcc ggaagcggat cctgcagtgg
     7261 tcgcgccgga atactggcgg gccgacctga cggttgccgg cattccccgc ttttccgggc
     7321 cgcagatcgt ggccgacgat gcggtgctgg atgcctggat gcggcagacg gcgcgctatg
     7381 gcctgtcgat cgtcgatggg ctgggcgacc agagcgatgc cggggtcgca gtggcgcagc
     7441 ggatcggctt cctgcgccag accaacttcg gcacgacctt tgaagtggtc agcatgcccg
     7501 atcccaacaa cctggcctac acgccggtgg ccctgccgct gcacaccgat ctgcccaacc
     7561 aggaagtgcc gccggggttt cagttcctgc attgcctggc caacgaagcc gaagggggcg
     7621 gatcgatctt tgccgatggc tttgccattg ccagcgatct gcggggccgc gaccccgaag
     7681 ccttccggat cctgagcgaa gtggcgatac cctatcgctt tcacgatgcc gattacgaca
     7741 tccgggtgcg cgaaccggtc atcacgctgg accgcgccgg tgagctggtc gagatccgct
     7801 acaacgcgca catcgccgcg atgttcgaca tgccggccga tctcatgccc gcgttctacc
     7861 gggcttaccg ggcctacatg gcgatgaccc gcgatcccca gtaccggctg tcgttcaagc
     7921 tggagagcgg cgaaatgatc gtgttcgaca accgccgcgt gctccacggg cgcgaggcgt
     7981 tcgatccttc gaccggctac cgccacctgc acggctgcta tgtcgatcgc ggcgagtttc
     8041 tctcacggct tcgggtcctg gcgcgagcct agcgcccctg ccagtgcgga gggcgtccct
     8101 cggcgaaggc cagggggccc tccagcgcgt cggccgaatc gagcatggcg cggtaggacg
     8161 ggatcgctcc gctgcgcatc agggcatagg cgcgctccac ctccaggccc tcggtcgcgc
     8221 gcaggatgtc gcgggcggcc ttgattgcca gcggggcacc cttggcaatc tctcgggcga
     8281 gctccagcgc ggtatcgagc agggcctcgg gctcgcacag gcggctggcc aggcccagcg
     8341 ccgcggcctc ggcggcatcg agccggcggc cggtcaggat catctcgcgc gccagttgcg
     8401 gcggcagcag cttgggcagg cgcagcaggc cgcccgaatc cggcaggatc cccagctgca
     8461 cttcgggcag ccagaaccgc gccgcggtgc tggccacgat caggtcggcc gccagcgcca
     8521 gttcgaagcc gccgcccacg gccacgccat tgaccgcggc gatgaccggc ttggacaggg
     8581 tgaagtactc ggtcagcccg gcaaagccgc ccggtccgtg atcggcatcg gctgcctcgc
     8641 cctcgttcgc ggcgttgagg tcccagccgg cggaaaagaa ccgtccggcc ccggtgatga
     8701 tccccacccg cagcgccgga tcctcgtcca gttcgcggaa cgcggcgtaa agctccagac
     8761 ttgtcgccac gtcgatcgcg ttggccttgg gccggtccag cgtgatgacc agcacttcgc
     8821 cgtgccgttc gagtttgacc gcgttcattc cggtgtttcc ctcatcctga tcagcgcgtc
     8881 gaccgctgtg gcaccgcggg gcagggccag caagggattg acgtccagct cttccagccg
     8941 gtcggcatgg tccagcgcga aggcggcaat ggcggtgatg gcattgacca gcgcgtcccg
     9001 gtcacagccg ggcttgccgc ggtagccctg cagcaccgca tcgatcggca gccgggccag
     9061 cgcggccgcg atctcttccc ggctggcggg cagcaacacc tgctcaacct ggcgcaacag
     9121 ttcgacgaaa atcccgcctg cgccgagcgt caggaacagg ccgaactgtg ggtcgcgggc
     9181 cacgccgaca atcagttccg ccacggcgcc gccgaccatc tcttcgatca ggaaggtgcc
     9241 gcccagcggg gccatgcgct gggcctcggc cagcaggcct tcgcggctgg tgatccccag
     9301 cgcaaccgcg ccgagttcgg tcttgtgggc caggtgctcg ccaaccgctt tcagcaccaa
     9361 ggggaacggg gcgggcaggg ggctggcggg atcggcgcac agggcctcga tctcggccag
     9421 ggtaacggtc cgtccggctg gaaccggcac cccggcgcgg gcgagggcgg ccttggagcg
     9481 aacctcgtcc cacaccgcgg ccgggccaga cagcgcggta aggggctcgg gcagggccgg
     9541 ggcggggggc cgtgaccaga cctctccgat cccggcggcc gcggcgatgg cggccagcgc
     9601 ttcagccagc cccgctgccg ggaccagtcc tgccgccagc aactgcgcgc agaccgcttc
     9661 gggcaggttt tccgccagcg aactcgccac gatccggacc gtgccgggct gcggtggatt
     9721 ggcgcggtgg gcgccgacga aagcgtcgag cgtcgtctgc caatcgcgcg catcgcagcg
     9781 gtccgcgcgc gggaaatcga ggatcaggat cgcggcttcg aaccccgccc cgatcagccc
     9841 ggcaaagcag cgtgtctggg ccggaccgtc gccccagatg taggtgtggt aatcaagcgg
     9901 gttggcgaca tggacttgcg ggcccagcac ctcctccaac tcggcctggc tggccggcgg
     9961 aaagtccggc gtttccaggc cggcccgttc ggccagatcg gcggtgagcg aggcctcgcc
    10021 gcccgaacag ctggcgctgg tgatccgccg cccggccagc ggcccggcca cgtgcagcag
    10081 cttcagcgtt tcgaggaact gcgccggatc gtgcgcccgg gcaacgccgt accgcgcgaa
    10141 cagcgcgtcg cacagcgcgt cggccccggc cagcgagctg gtgtgcgaaa gcgtcagcgc
    10201 cgcgcccttg gccgaggccc cggtcttcag cgcgaccagc ggcacgcccg cggcccgggc
    10261 ggcaaggcag gcctcggaaa agcgggcgac cgaaccgagg ccttccagat gcagcccgat
    10321 cgcactgacc cgctcatcac ggaccagcgc ctcgatcaga tcggccgggg tcacgtcggc
    10381 gcagttgccg acgctgatca cgtaaccgat cggcaggccg cgcgcctgca tggtgaagtt
    10441 gagcgccagg ttcccgctct ggctgatgat cgccacgccg cgcccttcgg caatgggcag
    10501 gcagccgtgc tgatccggcc acagcgcaac ccggtcaaag gcgttgagca tgccatagca
    10561 gttgggcccg accagcggca tggccccggc cgcgtccagc agttcggcct ggcgctgctg
    10621 gccctccggc ccggtctcgg caaagccgga ggcatagcag atcgctccgc cggcccccat
    10681 tgcggcgagg tctccggcca gggcgatagt cggcccggcc ggggcggcca ggaacacggc
    10741 atcgggcgcg tccggcaggt cgacgagggc gggcaggcag ggcacgccgg ccatgtcggc
    10801 gcggttcgga ttgaccggcc agatcgcccc gtcaaagccg aaggcgcggc tctggcgcac
    10861 ggcctcttcc gccgccttgc caccaacgaa ggcgatcgag cggggccgca gcaggcggtc
    10921 aagcggtttg gccgggctca tccctcaagc gggcgcagga tcgcgcggga aatgatgtgg
    10981 cgctggatct cgctggtgcc gtcccagatc cgctc
//
LOCUS       DASUDV010000186         8374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1464011,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000186 DASUDV010000000
VERSION     DASUDV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464011"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFV30_03915"
     CDS             <1..667
                     /locus_tag="VFV30_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847263.1"
                     /translation="WALGAVLLPLAAVLGGLAAQSAIAVMDVPEADASLSQAMNAALE
                     FDANGLRLLQIFNPGSVETIGTRNSYDQMEQTAMLHSLTSTLGGSGWLAPVDLLSMRK
                     EQAWDYVSAVHLMYPFGRIGVVAFLAVLFAAAFALGRPLLPGQRLAPDLPARPLFLRL
                     TGTMAVLTLAFSAAYMVLANLLMVPFTGRSIYLVSSLSTGDLLEGAIVMALAALAVTE
                     VRS"
     gene            664..3099
                     /locus_tag="VFV30_03920"
     CDS             664..3099
                     /locus_tag="VFV30_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847264.1"
                     /translation="MSQLLRSGEGGGTPQAKAKARGGQRLVWVLAALVLLAILVWPAV
                     RRAPDYGSNGAETGYPFLVRQLTDAGLLRFAERESVAGARRVVLDKNRLAELVEASAD
                     GEGPLGDCRRSPSPVGRFWRRLSGSVESSSAECEALIEAFIRASFLREDMRIDSGAIW
                     ESDGDRLVGLAPGAHMVSDATRSITQWQGSVDFAKPYQSLMLVDAATRKVLFRFDARQ
                     RNPVILSPAALLDDGAAELSGANAVLRARNRQCAGSATISQLGDHALLTLLPINAGSD
                     CPDIWFDGELVHSRDPKLGGFRYRVLRPGQTIRIMEDKPVVLQLVRSIGAVSALDEGR
                     RRSETTLAAVGTAISGAGPTRSRVPTSIDPVIHFTAQQRLEALGVRELAGERSSFRAA
                     AMLVDGLTGEIVAAPTFPISPNQLVESDRKRTLRLNWLGLNMNLQALDVGSTAKVPIA
                     TAIVQEHPQLLTLRTTGKSPGFTRLLGEPLVAGRNEEMEDSIGSGQTIDFPTFVARSS
                     NYYALLLMKLAASPQPLASNGKVLGPGEGYSIAGVARSRAPRMPANGEMWGMTWAEQL
                     WRLTCVMPYPIDKSADQRWARAGCPRAYYADPGITSPGRLAAIAHNSPALRLNYVQPG
                     LEYSDYLMSILGQSRSRWTNAALIQAYARILSDRQIGLRFDRPTAMELERRQPVPRLG
                     LNPQVWRNVTQGMAGAVEYGTAKRLKEALGPFPGLAVYAKTGTPTVPRVPGVNEVKEQ
                     GHVIVVAFVRYSGGVQDPANICAMRMVSINIQDRDGDEKTPALDLLLTLMGEPAIANW
                     LRAPCAPAGGRAP"
     gene            3096..3500
                     /locus_tag="VFV30_03925"
     CDS             3096..3500
                     /locus_tag="VFV30_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847265.1"
                     /translation="MSSSLLRSRGESGPGPVGRVSWLWLAALVLPALVAASLQEASQT
                     PPRKEFFDIGVPPQDRGWLCWTDGQGGCSAADFTRAYGLEAPQYRMVGEVVLERIGNA
                     GTYDPAKPILLKERRKCDQGDGAKCRGQVSGL"
     gene            3529..4413
                     /locus_tag="VFV30_03930"
     CDS             3529..4413
                     /locus_tag="VFV30_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847266.1"
                     /translation="MNTVAGGKSTGGAWGWVAILPLLALGGWQLGLFDGLGSKVETVD
                     QAWFDGYTDRFLSEETTVTVVSKANRRLYPTSQKTGVIGSYPTGARLRGRWVAGPDPA
                     ILWFKTSDGSYVWQGNLDGDRTVMAAAAKAAASGSAASEPANEAAVAAADAAAAASAA
                     ASAAPKASESAPAPAKAAAPAPAAGYDPAMAEAIRRAVPDAALAGEIVRNAEVFAPRD
                     SAGGRTLQWAICSTRCDFDFSTTVVLTGSGRRASAMVCVSDGRRMNGWSEWYVGGREA
                     GRTPGLCPSRLAAVKAPS"
     gene            complement(4406..5995)
                     /locus_tag="VFV30_03935"
     CDS             complement(4406..5995)
                     /locus_tag="VFV30_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX4847267.1"
                     /translation="MTDAEMQALIDRVAATRDLFTFDDFIRHWAKTTPNRSAIDSDDL
                     QLTYGELEAATARVASALLAMGLSKGDRIAWFGKNSGTYFTLFFGAARAGIVMTPVGW
                     RLAEPEAAFIIDNADAKVLFLGDGFEACKDTLGKRPGLTACLTSAEARGLIADAPRSD
                     FTPSGRHDAVLQLYTSGTTGNPKGAVLSNDNLFGLRKAAFDNPQAYNLWGEDEAVLVA
                     MPCAHIGGTGLGIMALSAGLPGVVLAEFDPPKVFDAVENRGVTRFFIVPAALQMLLNH
                     PDCAKTDFSRLKYILYGAAPIPLELLRQCIAMFGAGFIQAYGMTETTGTICMLPPEDH
                     SVEGNTRMRSAGKPLPGVEIEVRNSAGEALPVGEIGEICTRSSNNMLGYWKLPEATAS
                     TMSADGWVATGDVGYLDEDGYVYIYDRAKDMIISGGENVYPAEVESGIYGHPDVLEVA
                     VIGVPDDTWGEAVKAVCVPRPGHTIDEASVIGWARERLAGYKVPKSIDVIPALPRNAS
                     GKILRRELRAPYWEGRERQVN"
     gene            complement(6053..7519)
                     /locus_tag="VFV30_03940"
     CDS             complement(6053..7519)
                     /locus_tag="VFV30_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEX4847268.1"
                     /translation="MKKWVLGLGVALLALGGTVAARTAMFQPGEVADGSKITVAAAAP
                     FDVNRAAQNLGQAVRFRTVSNQDAAQNQTAEWDKLQAWLATTYPKAHAAMNQDKIGQT
                     LVYSWTGSDPAAQPVIMMAHQDVVPVTAGTEKDWKHDPFGGVIAEGAVWGRGTVDDKG
                     SLIALFEALEALAAQGFKPKRTIYLVSGHDEEVGGTGAAAAAKWLAKRKVKALFTLDE
                     GGLITTDTPVIGKPAMMIGVAEKGYVTLQVRAPAVGGHSSMPPAEIGTVNLSKAVVAI
                     HADQHPMELRGPVGGMIDVLASEAGGMTKVAAANKWLLGGQIISTMAKSPSSAAMLHT
                     TVAPTMLEGSPKENVLPQSASALINYRIAPWDSSAEVLKRARAAVEGLPVEVAITERG
                     AREPTPVSSSTSLGWQLIVASAQAGHKGVIAAPYLVVGGTDSRNMTPVSDDVYRFMPI
                     ALSTAETKMIHGTNEHMTLKNLESMIAFYTRLLATAAG"
     gene            complement(7583..>8374)
                     /locus_tag="VFV30_03945"
     CDS             complement(7583..>8374)
                     /locus_tag="VFV30_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4847269.1"
                     /translation="AINRSLAGRDGPLPILVAETGGQNAMIVDSSALPEQVTRDVVAS
                     AFQSAGQRCSALRVLYLQDDVYDGMLHMIRGAFEALVIGSPLDLATDVGPVIDAEAKS
                     KLDAHIAAMRSAGHTVWQRELPPHCAAGTFVAPAIIEIQSIADLENENFGPVLHVARF
                     KAEELEQVIAAINATGFGLTLGLHSRIDATRRLVQARARVGNFYVNRNQIGAVVGSQP
                     FGGEGLSGTGPKAGGPHYLARFATERVTCIDTTAAGGNASLMAAG"
BASE COUNT         1207 a         2884 c         2933 g         1350 t
ORIGIN      
        1 ctgggcgctg ggcgcggtcc tgctgccgct ggccgcggtg ctgggcgggc tggcggccca
       61 gtccgcgatc gcggtgatgg acgtgcccga ggccgatgcc agcctgtccc aggcgatgaa
      121 cgccgcgctg gaattcgatg ccaacgggct gcgcctgctg caaatcttca accccggctc
      181 ggtcgagacc atcggcaccc gcaacagcta tgaccagatg gagcagaccg cgatgctcca
      241 ttcgctgacc tccaccctgg gcggcagcgg ctggctggcg ccggttgatc tgctctcgat
      301 gcgcaaggag caggcctggg actatgtttc ggcggtgcac ctgatgtacc cgttcgggcg
      361 gatcggggtg gtggccttcc tggccgtgct gtttgccgcc gccttcgcgc tgggccggcc
      421 gctcctgccc gggcagcggc tcgctccgga cctgcccgcg cggccgctgt tcctgcgcct
      481 gaccgggacc atggcggtcc tgaccctggc cttcagcgcg gcctacatgg tgctggcgaa
      541 cctgctgatg gtgcccttta ccgggcgcag catctatctt gtttcctcgc tttccaccgg
      601 ggacctgctg gaaggcgcga tcgtgatggc gctggccgcg ctggcggtga cggaggtgcg
      661 gtcatgagcc agctgctgcg atcgggcgag ggcggcggca cgccgcaggc caaggccaag
      721 gcccgcggcg gccagcggct ggtctgggtg ctggccgcgc tggtcctgct ggcgatactg
      781 gtctggcccg cggtccgccg cgcgccggac tatggcagca acggggccga gacgggctat
      841 cccttcctgg tgcgccagct gaccgacgcc ggcctgctgc gctttgccga gcgcgagagc
      901 gtggccggcg cgcgccgggt ggtgctggac aagaaccggc tggccgagct ggtcgaggcc
      961 agcgccgacg gcgaggggcc gctgggcgat tgccggcgca gccccagccc ggtcggacgg
     1021 ttctggcggc ggctgagcgg atcggtcgaa tcgagcagcg ccgagtgcga ggccctgatc
     1081 gaagccttca tccgcgccag cttcctgcgc gaggacatgc ggatcgacag cggcgcgatc
     1141 tgggaaagcg atggcgaccg gctggtcggg ttggcccccg gcgcgcacat ggtgtccgat
     1201 gccacccgca gcatcaccca gtggcagggc tcggtcgatt tcgccaagcc ctaccagtcg
     1261 ctgatgctgg tcgatgccgc gacccgcaag gtgctgttcc ggttcgatgc gcgccagcgc
     1321 aatccggtga tcctttcgcc cgcggccctc ctggacgacg gcgcggccga gctgagcggg
     1381 gccaacgccg tgctgcgcgc ccgcaatcgc cagtgcgccg gcagcgccac gatctcgcaa
     1441 ctgggcgatc acgcgctgct caccctgctg ccgatcaacg cgggctcgga ttgcccggac
     1501 atctggttcg acggtgaact ggtccattcg cgcgatccca agctgggggg cttccgctac
     1561 cgcgtgttgc gtccgggcca gacgatccgg atcatggaag acaagccggt ggtgctgcag
     1621 ctggtgcgca gcatcggcgc ggtctcggcc ctcgacgaag gtcgccgccg gagcgaaacc
     1681 accctggccg cggtcggcac cgcgatctcc ggcgccgggc cgacccgcag ccgggtgccg
     1741 accagcatcg atccggtgat ccacttcacc gcgcagcaac ggctggaagc gctgggcgtg
     1801 cgcgaactgg cgggcgagcg cagcagcttc cgcgcggcgg ccatgctggt tgacgggctg
     1861 accggcgaga tcgtggccgc gccgaccttt ccgatcagcc cgaaccagct ggtggaaagc
     1921 gatcgcaagc ggaccctgcg gctcaactgg ctcggtctca acatgaacct ccaggcgctg
     1981 gatgtcggct ccaccgccaa ggttccgatt gccacggcga tcgtgcagga acacccgcag
     2041 ctcttgaccc tgcgcaccac cggcaagagc cccggcttca cccgcctgct gggcgagccg
     2101 ctggtggccg gccggaacga ggagatggag gattcgatcg gatcgggcca gaccatcgat
     2161 ttcccgacct tcgtcgcccg gtcgagcaac tattacgccc tgctgctgat gaagctggcg
     2221 gccagtccgc agccgctggc cagcaacggc aaggtgctgg gcccgggcga gggctactcg
     2281 atcgccggcg tggcgcgaag ccgggccccg cggatgccgg cgaacggcga gatgtggggg
     2341 atgacctggg ccgagcagct gtggcgcctg acctgcgtga tgccctatcc gatcgacaag
     2401 agcgccgacc agcgctgggc ccgggccggc tgcccgcgcg cctattacgc cgatcccggc
     2461 atcacctccc ccggccggct ggcggcgatc gcgcacaatt ccccggcgct gcggctcaac
     2521 tatgttcagc cggggctgga atactccgat tacctgatgt cgatcctcgg gcagagccgc
     2581 tcgcgctgga ccaacgccgc gctgatccag gcctatgccc ggatcctgtc cgaccggcag
     2641 atcggcctgc ggttcgaccg gccgactgcg atggagctgg agcgccgcca gccggtgccg
     2701 cgcctgggcc tcaacccgca ggtctggcgc aatgtcaccc agggcatggc cggagcggtg
     2761 gaatacggca cggccaagcg cctgaaggag gccctcgggc cgttcccggg cctggcggtc
     2821 tatgccaaga ccggcacgcc caccgtcccg cgggtgccgg gcgtgaacga ggtgaaggag
     2881 cagggccatg tgatcgtggt ggccttcgtc cgctattccg gcggggtcca ggatcccgcc
     2941 aacatctgcg cgatgcggat ggtgtcgatc aacatccagg accgcgacgg cgatgagaag
     3001 accccggcgc tcgatctgct gctgaccctg atgggcgaac cggcgatcgc caactggctc
     3061 cgcgcgccct gcgcgccggc cggggggagg gcgccatgag ttccagcctg ctgcgctcgc
     3121 ggggggaatc cggccccggt ccggtaggcc gggtttcgtg gctgtggctg gccgcgctgg
     3181 tcctgccggc gctggtcgcg gcctcgctcc aggaagcctc gcaaaccccg ccgcgcaagg
     3241 aattcttcga tatcggcgtt cccccgcagg accgcggctg gctgtgctgg accgacgggc
     3301 agggcggctg cagcgcggcc gatttcaccc gcgcctatgg gctggaagcg ccgcagtacc
     3361 ggatggtggg cgaagtggtg ctggaacgga tcggcaatgc cggtacttac gatccggcca
     3421 agccgatcct cttgaaggaa cggcgcaaat gcgatcaggg cgacggcgcg aaatgccgcg
     3481 gccaggtttc ggggctgtga cggcgatgac ggtccggggg gagacggcat gaacacggtg
     3541 gctggcggca aaagcacggg cggcgcgtgg ggctgggtgg cgatcctgcc cttgctggcg
     3601 ctgggcggct ggcagctggg gctgttcgac ggcctgggat cgaaggtcga aacggtcgat
     3661 caggcctggt tcgatggcta cactgaccgc ttcctgtccg aggaaaccac cgttaccgtg
     3721 gtcagcaagg ccaaccgccg gctctatccc acctcgcaga agaccggggt gatcggcagc
     3781 tatcccaccg gggcgcggct gcgcgggcgg tgggtggccg gccccgatcc ggcgatcctg
     3841 tggttcaaga ccagcgatgg cagctatgtc tggcagggca atctggacgg cgaccgcacg
     3901 gtcatggccg ccgcggccaa ggccgccgcc agcggttcgg ccgcatcgga gccggcgaac
     3961 gaagccgccg ttgccgccgc agacgctgcc gccgcggcaa gcgcggccgc ctcggccgcg
     4021 cccaaggcgt ctgaatccgc ccccgctccc gccaaggccg ccgcgccggc ccccgccgct
     4081 ggctatgacc cggccatggc cgaggcgatc cgccgggcgg tgcccgatgc ggcgctggcc
     4141 ggtgagattg tccgcaacgc cgaagtcttc gccccgcgcg acagcgccgg cggccggacc
     4201 ctgcaatggg cgatctgctc gacccgctgc gatttcgatt tttccaccac cgtggtgctg
     4261 accggctccg gccgccgcgc cagcgcgatg gtctgtgtgt ccgacgggcg gcggatgaac
     4321 ggctggtcgg aatggtacgt aggcggccgc gaggccggac gcacgcccgg cctttgcccg
     4381 tcgcggctgg cggcggtgaa agcgcctagt tgacctgccg ctcgcgccct tcccaatagg
     4441 gcgcgcgcag ttcgcggcgc aggatcttgc cgctggcgtt gcggggcagg gccgggatca
     4501 cgtcgatcga cttgggcacc ttgtatccgg ccagccgctc gcgcgcccag ccgatcacgc
     4561 tggcctcgtc gatcgtgtgg ccgggcctcg gcacgcagac ggccttgacc gcttcgcccc
     4621 aggtatcgtc gggcacgccg atcaccgcca cttccaacac gtcgggatgg ccatagatgc
     4681 cgctctcgac ctcggccgga tagacgttct ccccgccgga gatgatcatg tccttggccc
     4741 ggtcgtagat gtagacatag ccgtcctcat ccagatagcc gacgtcgccg gtggcgaccc
     4801 agccgtcggc gctcatcgtg ctggcggtgg cttcgggcag cttccagtac cccagcatgt
     4861 tgttggacga gcgggtgcag atttccccga tctcgcccac cggcagggcc tcaccggcac
     4921 tgttgcgaac ctcgatctcc accccgggca gcggctttcc ggccgaacgc atccgggtgt
     4981 tgccctcgac cgagtggtcc tcgggcggga gcatgcaaat cgtcccggtc gtctcggtca
     5041 tgccataggc ctggatgaag ccggcgccga acatggcgat gcattgccgc agcagttcca
     5101 gcgggatcgg cgcggcgcca tagaggatgt acttcaggcg gctgaaatcg gtcttggcgc
     5161 agtccggatg gttcagcagc atctgcagcg cggcgggcac gatgaagaaa cgggtgacgc
     5221 cgcggttctc caccgcgtcg aacaccttgg gcggatcgaa ttcggccagg accaccccgg
     5281 gcaggccggc ggacagggcc atgatcccca ggcccgtccc gccgatgtgg gcgcagggca
     5341 tcgccaccag cacggcctcg tcctcgcccc acaggttgta ggcctgcggg ttgtcgaacg
     5401 cggccttgcg gagcccgaac aggttgtcgt tggacaggac cgcgcccttg gggttgccgg
     5461 tcgtgccgct ggtgtaaagc tgcagcaccg catcgtggcg gcccgagggg gtgaaatcgc
     5521 tgcgcggcgc atccgcgatc agcccgcgcg cttcggccga ggtcaggcag gcggtcagcc
     5581 cggggcgctt gcccagggtg tccttgcaag cctcgaaccc gtcgcccagg aacaggacct
     5641 tggcatcggc gttgtcgatg atgaaggcgg cttccggttc ggccagccgc cagccgaccg
     5701 gggtcatcac gatcccggcc cgcgccgcgc cgaagaacag ggtgaaatag gtgccgctgt
     5761 tcttgccgaa ccaggcgatc cggtcccctt tgctgaggcc catggcgagc agcgcgctgg
     5821 ccacccgggc ggtcgccgcc tccagctcgc cataggtcag ctgcagatcg tcggaatcga
     5881 tcgcggaccg gttcggcgtg gtcttggccc agtggcggat gaaatcgtca aaggtaaaca
     5941 gatcgcgggt ggcagccacc cggtcgatca gggcttgcat ttcggcatcg gtcatcgcgt
     6001 tctcccgggc gcagtttaac cgcgcgatta acaagggcaa gcgctgtctt gcctaacccg
     6061 ccgcggtcgc cagcaggcgg gtgtagaacg cgatcatcga ttccaggttc ttcagcgtca
     6121 tgtgctcgtt ggtgccgtgg atcatcttgg tttcggcggt ggacagcgcg atcggcatga
     6181 agcggtacac gtcgtccgaa accggggtca tgttgcggct gtcggtgccg ccaaccacca
     6241 gatagggggc ggcgatcacg cccttgtgcc cggcctgggc cgaggcgacg atcagctgcc
     6301 agcccagcga ggtggaggag gataccgggg tcggctcgcg cgcgccgcgc tcggtaatcg
     6361 ccacttccac cggcaggcct tccaccgcgg cgcgggcccg cttcagcacc tcggccgagc
     6421 tgtcccacgg cgcgatccgg taattgatca gcgcgctggc gctttgcggc aggacgtttt
     6481 ccttgggcga tccttccagc atggtcggcg cgacagtggt gtggagcatg gcggccgagg
     6541 acgggctctt ggccatggtg gagatgatct ggccgcccag cagccacttg ttggccgcgg
     6601 cgaccttggt catcccgccg gcttcgctgg ccagcacgtc gatcatcccg ccgaccggcc
     6661 cgcgcagctc catcgggtgc tggtcggcat ggatcgcgac caccgccttg gagaggttga
     6721 ccgtgccgat ctcggccggc ggcatggagg aatgcccgcc caccgccggc gcgcggacct
     6781 gcagcgtgac atagcctttc tcggccacgc cgatcatcat cgccggcttg ccgatcaccg
     6841 gcgtgtcggt ggtgatcagc ccgccctcgt cgagcgtgaa cagcgccttg accttgcgct
     6901 tcgccagcca cttggccgcc gccgccgcgc cggtgccgcc cacttcctcg tcatggccgg
     6961 agaccagata gatcgtccgc ttgggcttga agccctgggc ggccagcgcc tccagcgctt
     7021 cgaacagcgc gatcagcgat cccttgtcgt ccaccgtgcc gcggccccag acggcgcctt
     7081 cggcaatcac cccgccaaag ggatcgtgct tccagtcctt ctcagttccg gcggtgacag
     7141 gcaccacgtc ctggtgggcc atcatgatca ccggctgggc cgccggatcg ctgcccgtcc
     7201 aggaatagac cagcgtctgc ccgatcttgt cctggttcat cgccgcatgg gccttgggat
     7261 aggtggtggc cagccaggcc tgcagcttgt cccattcggc ggtctggttc tgggcggcgt
     7321 cctggttgct gacggtcctg aaccgcaccg cctggcccag gttctgcgcc gcgcggttga
     7381 cgtcgaacgg ggcggctgcg gcgaccgtga tcttcgatcc gtcggccact tcgccgggct
     7441 ggaacattgc cgtgcgcgcg gccaccgtgc cgcccagcgc cagcaaggcc acgccaaggc
     7501 ccagaaccca cttcttcatc gctgtcccct ccatcccgtt tgcgcgcagg ctagggcgcg
     7561 gcgggcagag aggcaagcgg ggctatcccg ccgccatcag gctggcgttg ccgcccgccg
     7621 ccgtggtgtc gatgcaggtc acccgctcgg tcgcgaagcg cgccaggtag tgcggcccgc
     7681 cggccttggg gccggtgccg gacagccctt cgccgccgaa cggctggctg ccgaccaccg
     7741 cgccgatctg gttgcggttg acatagaagt tgccgacccg cgcccgggcc tggaccagcc
     7801 gccgggtcgc atcgatccgg ctgtgcagac ccagcgtcag gccaaaaccg gtggcgttga
     7861 tcgcagcgat cacctgctcc agctcctccg ccttgaaccg cgccacgtgg aggacggggc
     7921 cgaagttctc gttctccaga tcggcgatcg actggatctc gatgatcgcc ggggcgacga
     7981 acgtgccagc ggcgcagtgc ggcggcagtt cgcgctgcca gacggtgtgc cccgcgctgc
     8041 gcatcgcggc gatatgcgcg tcaagcttgg acttggcctc ggcatcgatc accgggccga
     8101 catcggtcgc cagatcgagc gggctgccga tcaccagggc ctcgaacgcg ccgcggatca
     8161 tgtgcagcat cccgtcgtaa acgtcatcct gcagatagag cacccggagc gcggaacagc
     8221 gttggcccgc gctctggaaa gcgctggcga cgacatcgcg ggtgacctgt tcgggcaggg
     8281 ccgaactgtc gacgatcatc gcgttctgcc cgccggtttc ggccaccagg atcggcagtg
     8341 gcccgtcgcg gccggccagg ctgcggttga tcgc
//
LOCUS       DASUDV010000187         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1221088,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000187 DASUDV010000000
VERSION     DASUDV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221088"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VFV30_03950"
     CDS             complement(<1..940)
                     /locus_tag="VFV30_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX4847270.1"
                     /translation="MEERELDDAERPPLSLPDLSPEARRERARLLKDTYDEHVGPHGR
                     FWQSRPFRVIARVWTGAYHDGFIHAGNLAYMAILALFPFFITVGAVFSLVGEQAQRAA
                     SVHTFLKALPPVVASALEPVAMDVIVARSGWLLWVGGLVGLWTVGSLIETIRDILRRA
                     YGTRETHAYWRYRLISTGVIFGAVIALLFALLAQVAIGTAQEVIQAWFPRAAGWIDTL
                     GTSRLVPALVIYLALYLLFFTLTPTAYRKRRYPKWPGALLVTAWWAAVSAALPAVLHR
                     FFTYDLTYGSLAGVMISLVFFWLVGLGMVVGAELNAA"
     gene            complement(954..1895)
                     /locus_tag="VFV30_03955"
     CDS             complement(954..1895)
                     /locus_tag="VFV30_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX4847271.1"
                     /translation="MADPYSILGVARGASEKDIKSAYRKLAKELHPDRNADNPKAAER
                     FSEVTRAYDLLSDKDKRARFDRGEIDIDGNPSMGFGYGGGGGPGGGFGGRDQRGFRAD
                     GFEGFGDGGIDLGDIFDGLFGGRGGMGGGMGGQRRGPAPRGANVQYKLGVSLPDAALL
                     ATQRITLADGKTIDLKLPAGVEDGTQMRLAGKGEPGPGGNGDALVVIHLQPHPFFRRD
                     GDNLRLDLPISLDEAVNGAKVKVPTPEGAVMLTVAPGTSSGKTLRLKGKGFTRKDGTR
                     GDQLVTIEVQLPEADEDLVRRLDGWRDPRNLRARLGV"
     gene            1998..2642
                     /gene="pdxH"
                     /locus_tag="VFV30_03960"
     CDS             1998..2642
                     /gene="pdxH"
                     /locus_tag="VFV30_03960"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX4847272.1"
                     /translation="MDETPMDETQAENAIPDAAPFALFDAWFADARKHELNDPEAVAV
                     ATATPDAFPSVRMVLLKDHGADLLGPQGGFVFYTNSHSRKGGELLANPKAAMLFHWKS
                     LRRQIRIEGTVARVPDEMADAYFASRSRDSQLGAHASDQSAPLGSRGEFLARIAKTTA
                     QHLIGKVPRPPHWTGFCLTPSAFEFWMDRPFRLHERRRFERLPDGGWTSTLLYP"
     gene            2639..>2887
                     /locus_tag="VFV30_03965"
     CDS             2639..>2887
                     /locus_tag="VFV30_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX4847273.1"
                     /translation="MTNHNALNRSAALASISVALLLVGLKVWAVFSTGSTAMLGSLAD
                     TALDLVASLATLIGVWVAAQPADEKHRFGHGKAEALAAM"
BASE COUNT          458 a         1025 c          974 g          430 t
ORIGIN      
        1 gggcggcgtt caattcggca cccaccacca ttcctaggcc aaccagccag aaaaagacaa
       61 gcgagatcat cacgcccgcg aggctgccat aggtcaggtc ataggtgaag aagcggtgca
      121 ggaccgccgg caaggccgcg ctgaccgcgg cccaccaggc cgtgaccagc agcgcgccgg
      181 gccacttggg atagcgccgt ttgcggtagg cggtgggggt cagcgtgaag aacagcaggt
      241 agagcgcaag gtagattacc aacgccggga ccagccgcga ggtgcccagc gtgtcgatcc
      301 agccggccgc gcgcgggaac caggcctgga tcacctcttg cgccgtgccg atcgcgactt
      361 gcgccagcag tgcgaacagc agcgcgatca ccgcgccgaa gatcaccccg gtggaaatca
      421 gccggtagcg ccaataggca tgcgtctcgc gcgtgccata ggcccggcgc aggatgtcgc
      481 ggatcgtctc gatcaggctg cccacggtcc acagcccgac cagcccgccg acccacagca
      541 gccagccgct gcgcgccacg atcacgtcca tcgccaccgg ttccagcgcc gaagcgacga
      601 ccggcggcag cgccttcagg aaggtgtgga ccgatgcggc gcgctgggcc tgttcgccga
      661 ccaggctgaa caccgcaccc accgtaatga agaacggaaa cagcgccagg atcgccatgt
      721 aggccaggtt gccggcgtgg atgaagccgt cgtggtaggc cccggtccag acccgggcga
      781 tcacccggaa cgggcgcgat tgccagaacc ggccgtgcgg gccgacatgc tcgtcatagg
      841 tatccttgag cagccgggcc cgttcgcgcc gggcctcggg cgagaggtcc ggcagcgaca
      901 gcggcggccg ctcggcatcg tcgagttcgc gttcctccac ggcgcggccg gtcctagacg
      961 cccagccgcg cgcgcaggtt gcgcggatcg cgccagccgt ccagccggcg gaccaggtcc
     1021 tcgtcggcct cgggcagctg gacctcgatt gtcaccagct ggtcgccgcg ggtcccgtcc
     1081 ttgcgggtaa agcccttgcc cttcaggcgc agggtcttgc ccgaactggt cccgggggcg
     1141 accgtcagca tcaccgcgcc ttccggggtc ggaaccttga ccttggcccc gttcaccgcc
     1201 tcatccaggc tgatcggcag gtcgagccgc aggttgtcgc cgtcgcggcg gaagaaggga
     1261 tgcggctgca ggtggatcac caccagggca tcgccgttgc cgccgggccc gggctcgccc
     1321 ttgccggcga ggcgcatctg ggtgccgtcc tcgaccccgg cgggcagctt caggtcgatc
     1381 gtcttgccgt cggccagggt gatccgctgg gttgccagca gggccgcgtc gggcagcgaa
     1441 acacccagct tgtactgcac gttggcgccg cgcggggccg ggccgcggcg ctggccgccc
     1501 atgccgcccc ccatgccgcc acgcccgccg aacaggccgt cgaaaatgtc gccgaggtcg
     1561 atcccgccat cgccgaaccc ttcgaacccg tcggcgcgga agccgcgctg gtcgcgcccg
     1621 ccaaatccgc cgccaggacc gcccccgccg ccatagccga agcccatgct ggggttgcca
     1681 tcgatgtcga tttcgccccg gtcgaaccgg gcgcgcttgt ccttgtccga cagcaggtca
     1741 taggcgcggg tcacttcgga aaagcgctcc gccgccttgg gattgtccgc gttgcggtca
     1801 gggtgcagct ctttcgccag cttgcgatag gcggacttga tgtccttctc gctcgccccg
     1861 cgggcaacac ccaggatcga atagggatcg gccatggggc ctagctaggt gtgttaggcc
     1921 catagggcaa gggcagcgcc ccttgttgcg agacgctcgc gcttccttcg cgggcgctgc
     1981 gcggctatgc gcggggcatg gacgaaaccc ccatggacga aacccaggcc gagaacgcaa
     2041 tccccgatgc ggcgcccttt gcgctgttcg acgcgtggtt tgccgatgcg cgcaagcatg
     2101 agctgaacga tcccgaggcg gtggccgtcg ccacggccac gccggacgcc tttccgtcgg
     2161 tgcggatggt gctgctgaag gaccacgggg ccgatctgct cggtccgcag ggcggcttcg
     2221 tgttctacac caattcgcac agccgcaagg gcggggaact gctggccaac cccaaggcgg
     2281 cgatgctgtt ccactggaaa tcgctgcgcc gccagatccg gatcgaggga accgtggcgc
     2341 gggttccgga tgaaatggcc gatgcctatt tcgccagccg cagccgggat tcgcagcttg
     2401 gcgcccacgc ctcggaccag tccgcgccgc tcggctcgcg cggggaattc ctcgcccgga
     2461 tcgccaagac gaccgcgcag cacctgatcg gaaaggttcc gcgcccgccg cactggaccg
     2521 gcttctgcct gacccccagc gccttcgaat tctggatgga ccgcccgttc cgcctgcacg
     2581 aacgccgccg gttcgaacgg ctgcctgatg gcggctggac cagcacgctg ctctacccat
     2641 gaccaaccac aacgccctca accgctcggc ggcgctggcc tcgatttcgg tcgcgctgct
     2701 gctggtcggg ctcaaggtct gggccgtgtt ctcgaccggg tcgaccgcca tgctcggcag
     2761 cctggccgat accgcgctcg atctggtggc cagcctggcc acgctgatcg gcgtctgggt
     2821 cgcggcgcag ccggccgacg agaagcaccg cttcgggcac ggcaaggccg aagcgctggc
     2881 cgcgatg
//
LOCUS       DASUDV010000188         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1618476,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000188 DASUDV010000000
VERSION     DASUDV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618476"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFV30_03970"
     CDS             <1..333
                     /locus_tag="VFV30_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX4847274.1"
                     /translation="LRAPLNPNAQQRWANPVFGERLKAVAEDLGLYRIMLNRGYTPVE
                     LDQCLADEALAKSHEVATQYAFEKLGVQGTPSFMINGQLQNVHSWPELEQALNAAVAA
                     RKTPVEKR"
     gene            413..1162
                     /locus_tag="VFV30_03975"
     CDS             413..1162
                     /locus_tag="VFV30_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX4847275.1"
                     /translation="MTIRRSLLAVAALPLALGLAACGAKEGEGAPPKGEAIAKIAPPA
                     GKTWQDVIEETADGGMRMGNPEAPIKLIEYGSLTCPHCAKLSQEGFAPLVSNYVASGR
                     VSFEFRSFAIHPQDVPLTVLARCSSKEAFFPLIEQVYTNFDAINAPFQDEAVLQRAQA
                     TMQLPPEQRWSAFADAVGYTQFFAARGVAVDQAKACLADLPKAKQVADHAQKYSADGI
                     TGTPALVLNGVKLDTNTWSGLEVALQNAGAR"
     gene            1196..>3310
                     /locus_tag="VFV30_03980"
     CDS             1196..>3310
                     /locus_tag="VFV30_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4847276.1"
                     /translation="MRIKRLKLSGFKSFVEPAELRIEPGLTGVVGPNGCGKSNLLEAI
                     RWVMGESSPKSMRGGGMEDVIFAGTAQRPPRDFAEVVLYADTADNDSELEVVRRIERG
                     AGSAYRVNGRDVRAKDVALVFADAATGAHSPALVSQGKIAAVIAAKPAERRMMLEEAA
                     GIAGLHVRRKDAEQKLRATEANLARLEDLMGQLDQQIGSLRRQARAAERYKALSDRIR
                     IAEARLVFARWRDAAAAAEAARAEAQGAEARVAAAQEAMKAAQQAQHQAAEALAEARE
                     ELADRRDDATAHGHRMAALTSQLEAAEERLKDLERQHRRLEEDRGEADRLTRDAAEAL
                     ARLERELAEGEEQLAADERRRPVVAAALDDADRNLRQAELALAKATADQAGVEAEWRV
                     ADAELAAAQQRLTRLESEARRQEEIKAALEREGDPLAALAEAEAARDAAASALEAARA
                     ALESQQARKSELQASRDATSSALATARAELAGIEREHGALVRDRDARARQASGKHGLP
                     IALDQVRAAPGYERALAAVLGRDAKAPLGQAPADEDGRFWTGAAAPIPVKDSLAAHVS
                     QCPAELKARLALVHVADADDGRALGPGEWLVTRGGRIRRWDGFVAKGEGAAEAARLEA
                     ENRLASLEAQLPALCQAVEQADAAAQAVQAELAELQVGLIAAERAVAQAAEAERAALR
                     ACDQAEAAKARLEARRVELAAAA"
BASE COUNT          575 a         1162 c         1139 g          434 t
ORIGIN      
        1 ctgcgcgcgc cgctcaatcc caatgcccag cagcgctggg ccaatccggt gttcggcgaa
       61 aggctcaagg cggtggccga agacctcggg ctctaccgga tcatgctcaa ccggggctat
      121 accccggtcg aactggacca gtgcctggcc gatgaagccc tggccaagag ccacgaagtg
      181 gccactcagt acgcctttga aaagctcggc gtgcagggaa ctcccagttt catgatcaac
      241 ggccagctgc aaaacgtcca ttcctggccc gaactggaac aggcgctcaa cgccgccgtg
      301 gcggcgcgca agactcctgt ggagaagcgc tgagccgata accgccttcc ttcatcccgg
      361 cccaggcttg ggctagtttt cacgactcga tattcaaagg aatccgcctg ccatgactat
      421 ccgccgtagc ctccttgccg ttgctgctct cccgctggcc ctgggccttg ccgcctgtgg
      481 cgccaaggaa ggcgaaggcg cgccgcccaa gggcgaagcg atcgccaaga ttgccccgcc
      541 ggccggcaag acctggcagg acgtgatcga ggaaaccgcc gatggcggga tgcgcatggg
      601 caaccccgaa gcgccgatca agctgatcga atatggttcg ctgacctgcc cgcactgcgc
      661 caagctttcg caggaaggct ttgctccact ggtcagcaac tatgtcgcca gtggccgggt
      721 gagcttcgaa ttccgcagct tcgcgatcca tccccaggat gtgccgctga ccgtgctggc
      781 gcgctgctcg agcaaggaag ccttcttccc gctgatcgag caggtttata ccaatttcga
      841 cgcgatcaac gcgccgttcc aggacgaggc cgtactgcag cgcgcccagg ccaccatgca
      901 gctgcccccg gaacagcgct ggagcgcgtt tgccgatgca gtgggctata cccagttctt
      961 cgccgcccgc ggcgtggcag tcgatcaggc caaggcctgc cttgccgatc tgcccaaggc
     1021 caagcaggtt gccgatcacg cccagaagta cagcgccgac gggatcaccg gaaccccggc
     1081 cctggtgctc aacggggtca agctcgatac caacacctgg agcgggctcg aagtcgcgct
     1141 gcagaacgcg ggcgcccgct aaggcggcag gcaaggcgag gttcggcagc ccccgatgcg
     1201 gatcaaacgg ctcaagctca gtggcttcaa aagcttcgtc gagcctgccg agttgcgcat
     1261 agaaccgggg ctgaccgggg ttgtcgggcc caacggctgc ggcaagtcca acctgctcga
     1321 ggcgatccgc tgggtgatgg gcgaaagcag ccctaaatcg atgcgcggcg ggggcatgga
     1381 agacgtgatc ttcgccggaa ccgcgcagcg cccgccgcgc gatttcgccg aagtcgtgct
     1441 ctatgccgat acggccgaca acgacagcga actcgaagtg gtccgccgga tcgagcgcgg
     1501 cgcaggttcg gcctatcggg tcaatggccg cgatgtgcgc gccaaggacg tggcgctggt
     1561 cttcgccgat gccgccaccg gtgcgcactc ccccgcgctc gtcagccagg gcaagatcgc
     1621 cgcggtcatt gccgccaagc ccgccgaacg gcggatgatg ctggaagagg cggccgggat
     1681 cgccgggctg cacgtgcgcc gcaaggacgc cgaacagaag ctgcgcgcga ccgaggcgaa
     1741 ccttgcccgg cttgaagacc tgatgggcca gctcgatcaa cagatcggct cgctgcgccg
     1801 ccaggcccgc gccgccgaac gctacaaggc gctgtccgac cggatccgga tcgccgaggc
     1861 gcggctggtc ttcgcccgct ggcgcgatgc cgccgccgcc gccgaagccg cccgggccga
     1921 agcccagggc gccgaagccc gcgttgccgc cgcgcaggaa gcgatgaagg cggcgcagca
     1981 ggcccagcac caggccgccg aggcgctggc cgaagcccgc gaggaactgg ccgaccggcg
     2041 cgacgatgcc actgcccacg gccaccgcat ggcggcgctg accagccagc tcgaagcggc
     2101 cgaggaacgc ctgaaggatc tcgaacgtca gcaccggcgg cttgaggaag accgcggcga
     2161 ggccgaccgg ctgacccgcg atgccgccga agcgctggcc cggctcgaac gcgaactggc
     2221 tgaaggcgag gaacagcttg ccgccgacga acgccgccgc ccggtggttg ccgccgcgct
     2281 ggacgatgcc gaccgcaacc tgcgccaggc cgaactggcg ctggccaagg ccacggccga
     2341 ccaggccggg gtcgaggccg aatggcgcgt cgccgatgcc gaactggccg ccgcgcagca
     2401 gcgcctcacc cggcttgaaa gcgaggcgcg ccgccaggag gagatcaagg ccgcactgga
     2461 acgcgagggc gatccgctgg ccgccctggc cgaagccgaa gccgcgcgcg atgctgccgc
     2521 aagcgcgctc gaagccgccc gcgccgctct ggaaagccag caggcccgca agagcgaact
     2581 gcaggccagc cgcgatgcca cctcctcggc gctcgccacc gcgcgcgccg aactggccgg
     2641 gatcgagcgg gagcacggcg cgctggtgcg cgatcgcgat gcccgggcca ggcaggccag
     2701 cggcaagcac ggcctgccga tcgcgctcga ccaggtccgc gccgcgccgg gttatgagcg
     2761 tgcgctcgcc gcggtgcttg gccgcgatgc caaggcgccg ctgggccagg ccccggcgga
     2821 tgaggatggc cggttctgga ccggtgccgc tgctcccatt ccggtgaagg acagccttgc
     2881 cgcccatgtc agccagtgcc cggccgagct caaggcccgg ctcgcgctgg tccacgtcgc
     2941 cgatgccgat gacggccgcg cattgggacc gggcgaatgg ctggtcaccc ggggtgggcg
     3001 gatccgccgc tgggacggct tcgttgccaa gggtgaaggc gccgccgaag ctgcccggct
     3061 cgaagccgaa aaccgccttg cctcgcttga agcgcagctt cccgcgctgt gccaggcggt
     3121 ggaacaggcc gatgccgcgg cccaggccgt gcaggctgaa ctggccgagc tgcaggtcgg
     3181 tctgatcgct gcggaacgtg cggtggccca ggcggcagag gccgaacgcg ccgcgctgcg
     3241 tgcctgcgac caggccgaag ccgccaaggc ccggctcgaa gcgcgccggg tcgaactggc
     3301 cgctgccgca
//
LOCUS       DASUDV010000189         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1685011,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000189 DASUDV010000000
VERSION     DASUDV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685011"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFV30_03985"
     CDS             complement(<1..259)
                     /locus_tag="VFV30_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847277.1"
                     /translation="MKLIIGNKNYSSWSLRGWLAVKQSGLHFEEITVPLYGEDWDARK
                     KQTEGIEPSSGKVPVLWDGEAVVWDSLAIIEYLSDKVGRERF"
     gene            308..727
                     /locus_tag="VFV30_03990"
     CDS             308..727
                     /locus_tag="VFV30_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4847278.1"
                     /translation="MTLRPFHLAFPVRDLAEARAFWGGVMGCAEGRSSDQWIDFDFYG
                     HQIVAHCTGQAADDAGSNPVDGHGVPVPHFGIVLSLPDWEALAQRLKAAGVTFEIEPY
                     VRFKGQPGEQATMFFRDPSGNAIEMKAFADLGQLFAR"
     gene            complement(753..1394)
                     /gene="yihA"
                     /locus_tag="VFV30_03995"
     CDS             complement(753..1394)
                     /gene="yihA"
                     /locus_tag="VFV30_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX4847279.1"
                     /translation="MSEDPDLVEAARKLFAGRVDFLLSAPALKFLPDPGAPEIAFAGR
                     SNVGKSSLLNALTGRKSLARASVTPGRTQELNFFEVGEPTRIRLVDMPGYGYAKAPLK
                     VVEAWKRLVRDYLRGRVELKRTLVLIDARHGVKDVDREMLKMLDEAAVGYRLVLTKAD
                     KIKASELEAVLAATQAEARKHPAAYPVVHVTSSEKGMGIAELRGAVLADASIA"
     gene            complement(1398..3182)
                     /gene="yidC"
                     /locus_tag="VFV30_04000"
     CDS             complement(1398..3182)
                     /gene="yidC"
                     /locus_tag="VFV30_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4847280.1"
                     /translation="MDNQNSRNMILAGILTVTILLGWDQAMRYFYPKANAPAPAATAS
                     ATPAPVESGTAKKTREGGLTNPADIALEAKDIKTVLANPARVKIEAPGLSGSINLAGG
                     VIDDLSTNRHTAALDQGQGLQRIYSPAGTPAQQYAQFGWTMGDGAPAIALPTAQTVWT
                     APAGAKLTPQTPVTLTWNNGAGQTFSLTYAIDADYMLTVSQTVSNAGPAPVTLKPVAQ
                     LSRTDKTAALDSFNVHAGPFGAFDGVVNFGNDYDDVAEAGTVALAGKPDWVGFTDIYW
                     MSTLIPEKPGATADFRALQNGVYLANLFYPQAGIAPGQQTTRVTKLFAGAKETDVLDR
                     YEASGIANFSLAIDWGWFRWFEKPLFALLKWLFGAVGNFGVAIMLLTVIVKAVMFPVS
                     QKQFASMAAMKAIQPKMKALQDRYKDDKAKQQQEIMDLYKKEGVNPLAGCLPIFLQIP
                     VFFALYKVLILSVEMRHQPFVLWIKDLSAPDPLHFLNLFGYLNFTVPAFLGIGVLALL
                     LGITMWLQFKLNPAQMDPVQQQVFAIMPWLMMFIMAPFAAGLLLYWITNNVLTIAQQS
                     YLYSKHPQLKAQAEKDAEDSKRAQARGT"
     gene            complement(3240..3449)
                     /gene="yidD"
                     /locus_tag="VFV30_04005"
     CDS             complement(3240..3449)
                     /gene="yidD"
                     /locus_tag="VFV30_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX4847281.1"
                     /translation="MKRLLILIARGWQLGPSRVLPPSCRYAPSCSQYAIEAVEKHGAI
                     KGGWLAFKRIMRCHPWGGHGYDPVP"
     gene            complement(3446..3742)
                     /gene="rnpA"
                     /locus_tag="VFV30_04010"
     CDS             complement(3446..3742)
                     /gene="rnpA"
                     /locus_tag="VFV30_04010"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX4847282.1"
                     /translation="MARPGFVLLVNPNGTGTVRYGITVTKKIGNAVVRNRMKRRFRAL
                     LREALPTAGLPAHDHVLIGREGGVERDFAKLREELAMALSRAAEGKGDPPRKRK"
BASE COUNT          601 a         1223 c         1247 g          673 t
ORIGIN      
        1 agaaccgttc acgcccgacc ttgtcggaca ggtactcgat gattgccagg ctgtcccaca
       61 ccacggcctc gccgtcccac agcaccggga ccttgcctga tgacggctcg atcccctcgg
      121 tctgcttctt gcgagcgtcc cagtcctcgc cataaagcgg gacggtgatc tcctcgaaat
      181 ggagacccga ttgcttgacc gccagccagc cgcgcagcga ccagctggaa tagttcttgt
      241 tgccgatgat cagcttcatg ggttagcggc taaccattca tcttcaccct gtcgagtctg
      301 aacagccatg accctgcgcc ccttccacct ggcctttccc gtccgcgatc tggccgaggc
      361 ccgcgccttc tggggcggag tgatgggctg cgccgaaggg cgatcgtccg accagtggat
      421 cgatttcgat ttctacggcc accagatcgt cgcccattgc accgggcagg cggcggacga
      481 tgccggcagc aacccggttg acggccacgg cgtgccggtg ccgcatttcg gtatcgtgct
      541 cagcctgccg gattgggagg cgctggccca gcggctcaag gcggccggcg tcaccttcga
      601 gatcgagccc tatgtccgct tcaagggcca gccgggcgaa caggccacga tgttcttccg
      661 cgatcccagc ggcaacgcga ttgaaatgaa ggccttcgcc gatcttggcc agctgttcgc
      721 gcgctgaggc accggggccc tgaagcgggg ccctaggcga tcgaggcgtc ggccagcacc
      781 gcgccgcgca gttcggcgat gcccatgccc ttttcgctgc tggtgacgtg gaccaccgga
      841 taggcggcgg gatgcttgcg ggcctcggcc tgggtggcgg ccagcacggc ttccagttcg
      901 ctggccttga tcttgtcggc cttggtcagg accagccggt aaccgacggc ggcttcgtcc
      961 agcatcttga gcatttcgcg gtcgacgtcc ttcaccccgt ggcgggcgtc gatcagcacc
     1021 agggtgcgct tcaactcaac ccggccgcgc aggtaatcgc gcaccagccg cttccacgcc
     1081 tcgaccacct tgagcggagc cttggcatag ccatagcccg gcatgtcgac caggcggatg
     1141 cgggtcggct cgcccacttc gaagaagttc agttcctgcg tgcgccccgg ggtgaccgag
     1201 gcgcgggcga gcgacttgcg cccggtcagc gcgttcagca ggctgctctt gcccacgttc
     1261 gagcgcccgg caaaggcgat ctccggcgcg cccggatcgg gcaggaactt cagcgccggc
     1321 gcgctgagca ggaagtccac ccgcccggca aagagcttgc gcgcggcttc gacaagatcg
     1381 ggatcctcgc tcacggctca ggtgccgcgc gcctgggccc gcttggagtc ttccgcgtcc
     1441 ttttccgcct gggccttcag ttgcgggtgc ttggaataga ggtagctctg ctgggcaatg
     1501 gtcagcacgt tgttggtgat ccagtagagc agcagcccgg ccgcgaaggg cgccatgatg
     1561 aacatcatca gccacggcat gatcgcgaag acctgctgct ggaccgggtc catctgggcc
     1621 gggttcagct tgaactgcag ccacatggtg atccccagca gcagcgccag gacgccgatc
     1681 cccaggaagg ccggaacggt gaagttcaga tagccgaaca ggttgaggaa gtgcagcgga
     1741 tcgggcgcgg agaggtcctt gatccacagc acgaagggct ggtggcgcat ttcgaccgag
     1801 aggatcagca ccttgtagag cgcgaagaac accgggatct gcaggaagat cggcagacag
     1861 cccgccagcg ggttaacgcc ttccttcttg tagagatcca tgatctcctg ctgctgcttg
     1921 gccttgtcgt ccttgtagcg gtcctgcagc gccttcatct tcggctggat cgccttcatc
     1981 gcggccatgc tggcgaactg cttctgcgaa accgggaaca tcaccgcctt gacgatgacg
     2041 gtcagcagca tgatcgccac gccgaagttg ccaaccgcgc caaacagcca cttgagcagc
     2101 gcgaacagcg gcttttcgaa ccagcggaac cagccccagt cgatcgccag gctgaagttg
     2161 gcgatgccgg acgcctcgta acggtccagc acgtcggttt ccttggcccc ggcgaacagc
     2221 ttggtcaccc gggtggtctg ctgccccggc gcgatgccgg cctgcggata gaacaggttg
     2281 gccagataga cgccgttctg cagcgcgcgg aaatcggcgg tcgcgcccgg cttttcgggg
     2341 atcagggtgg acatccagta gatgtcggta aagcccaccc agtcgggctt gccggccagg
     2401 gcgaccgtgc cggcctcggc cacgtcgtcg taatcgttgc cgaagttgac caccccgtca
     2461 aaggcgccga acggcccggc gtggacgttg aagctgtcga gcgcggcggt cttgtcggtg
     2521 cggctgagct gggcgaccgg cttgagcgtg accggcgcgg gaccggcgtt gctgacggtc
     2581 tggctgacgg tcagcatgta atcggcatcg atcgcatagg tcaggctgaa ggtctgcccg
     2641 gcgccgttgt tccaggtcag ggtgaccggg gtctgcgggg tcagcttggc gccggccggg
     2701 gcggtccaga ctgtctgcgc ggtcggcagg gcaatcgccg gggcgccatc gcccatggtc
     2761 cagccgaact gggcatactg ctgcgccggg gtgccggccg gcgaatagat ccgctgcagc
     2821 ccctggccct gatcgagcgc ggcggtgtgg cggttggtgc tgagatcgtc gatcaccccg
     2881 ccggccagat tgatcgagcc cgacaggccc ggcgcctcga tcttcacccg cgccggattg
     2941 gccagcacag tcttgatgtc cttggcttcc agcgcgatgt cagccgggtt ggtgaggccg
     3001 ccttcgcggg tcttcttggc cgtgccgctt tccaccgggg ccggggtggc gctggccgtc
     3061 gcggccggcg cgggggcatt ggccttcgga tagaaatacc gcatcgcctg gtcccagccg
     3121 agcaggatcg tcacggtcag gatcccggcc aggatcatgt tgcggctgtt ctggttgtcc
     3181 actgggggcc cctgtcagga aatggcgttc taggtcagat ggggaaggtt cggcctcggt
     3241 caaggcaccg ggtcgtaacc gtgccctccc cacggatggc agcgcattat acgcttgaag
     3301 gccaaccagc cacccttaat cgcaccgtgt ttctcgaccg cctcgatcgc gtattgcgag
     3361 cacgaggggg catagcggca cgacggcggc aggacccgcg agggccccag ctgccagccg
     3421 cgggcgatca agatgaggag ccgcttcact tgcgtttcct tggcggatcg cccttgcctt
     3481 cggcggcgcg cgacaaggcc atggccagtt cctcgcgcag cttggcgaaa tcgcgctcaa
     3541 ccccgccttc gcggccgatc agcacgtgat cgtgggcggg caggccggcg gtgggcagcg
     3601 cctcgcgcag cagcgcgcgg aaccggcgct tcatccggtt gcggaccacc gcgttgccga
     3661 ttttcttggt cacggtgatg ccgtaacgga ccgtgccggt cccgttcggg ttcaccaaaa
     3721 gcacaaaacc ggggcgcgca atcc
//
LOCUS       DASUDV010000190         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1685046,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000190 DASUDV010000000
VERSION     DASUDV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685046"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..539)
                     /locus_tag="VFV30_04015"
     CDS             complement(198..539)
                     /locus_tag="VFV30_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4847283.1"
                     /translation="MRRIDPSAPVTEPASPPADRLTAGQPIGTARTDYESPDGRFSAG
                     VWTCTPGRWRIAYDENEYCRILSGKGAVIDADGSVQAIGPGDEFVIPAGFKGEWLVEE
                     TMTKTWVIALP"
     gene            complement(526..1167)
                     /locus_tag="VFV30_04020"
     CDS             complement(526..1167)
                     /locus_tag="VFV30_04020"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="d(CMP) kinase"
                     /protein_id="HEX4847284.1"
                     /translation="MIIAVDGPTASGKGTIAKALSAHFGLPHLDTGLLYRAVGRQCAL
                     NHGNADDPLDALAACDFPDRLLTDPELRSEASGGLASRVSVHPEVRRALFERQRSFAL
                     QPGGAVLDGRDIGTVIAPEANVKLFVVASVPARAERRFREMQAAGRSVTLAEIAADLE
                     ARDARDRGRADAPLVAASDAVLLDTSDLGRDEAVAAAIAVVEARRKERRDAQD"
     gene            complement(1164..1427)
                     /locus_tag="VFV30_04025"
     CDS             complement(1164..1427)
                     /locus_tag="VFV30_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX4847285.1"
                     /translation="MIALDWATLVGFAGTACIIAAYAYLTVNDEPNPFVLHLTNLAGA
                     ALLTVSLLVHMNLASLVLEGFWAAIAIYGLVKALRQARQEPQP"
     gene            complement(1424..>2204)
                     /locus_tag="VFV30_04030"
     CDS             complement(1424..>2204)
                     /locus_tag="VFV30_04030"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX4847286.1"
                     /translation="AGLNTPGITTVIEPVPTRDHSERMLRGFGAQLDVEEVNGERIIR
                     IHGEAELSPQTIEVPGDPSSAAFFMVAALIVPGSELTIENVGLNPTRAGLVHVLRQMG
                     GDITEVNAREVGGEPVADLVVKHSALKGIAVDPAVAPSMIDEFPVLFVAAALAEGTTV
                     TTGLEELRVKESDRITAMAEALALAGARVRETGDDLIIEGTGGELLAGGGPVTTHLDH
                     RIAMSMAVAGLASRAGVEVDDTRPIATSFPVFEALLDGATR"
BASE COUNT          352 a          741 c          758 g          353 t
ORIGIN      
        1 gggcgaaccc tggttggcgg cgatcccgtg gcaggccgcg caatgctgct gcgccagggt
       61 cagcccctgc ttggccgatc gggaaagtcg gggcggtgtg ctggccgcga ccgcggcgag
      121 ggaggagacg agcagggcgg cgacaagcac gatacgcatg gcaaggctct atcaccgcgc
      181 gcacactgca ggcaagttca gggcagggcg atcacccagg tcttggtcat ggtctcttcg
      241 accagccatt cgcccttgaa gcctgcaggg atcacgaatt catcgcccgg gccgatcgcc
      301 tggacgctgc cgtcggcatc gatcaccgcg cccttgcccg acaggatccg gcagtattcg
      361 ttctcgtcgt aggcgatccg ccagcgtccc ggggtgcagg tccagacgcc tgccgagaag
      421 cgcccgtcgg gggattcgta atcggttcgc gcggtgccga tgggctggcc tgcggtaagg
      481 cgatcagcgg gcgggctggc gggttcggtg accggggctg aaggatcaat cctgcgcatc
      541 gcgtcgttcc ttgcggcggg cttcgaccac ggcaatggcc gcagccaccg cttcatcgcg
      601 tcccaggtcg gaggtatcga gcagcaccgc gtcgctcgcg gcgaccagcg gggcatcggc
      661 gcggccgcga tcgcgggcat cgcgggcttc cagatcggcg gcgatttcag ccagggtcac
      721 gctgcgcccg gcggcctgca tctccctgaa acggcgttcg gcccgggccg gaaccgaggc
      781 gacgacgaac agcttcacat tggcctctgg tgcgatcacc gtgccgatat cgcgcccgtc
      841 gagcaccgcg ccgcccggct gcagggcgaa gctgcgctgg cgttcgaaca gcgcgcggcg
      901 cacctcgggg tggaccgaaa cccggctggc aaggcctccg ctcgcttcgc tgcgcagttc
      961 gggatcggtc agtagcctat cgggaaaatc gcaggccgcc agcgcgtcga gcggatcgtc
     1021 ggcattgccg tggttgagcg cgcactgccg cccgaccgcg cgatagagca gcccggtatc
     1081 aaggtgcggc aggccgaaat gggctgacag cgccttggca atcgtgcctt tgcccgatgc
     1141 ggtggggcca tcgaccgcga tgatcacggc tggggctcct gcctggcctg gcgcaacgcc
     1201 ttgaccagcc cgtaaatcgc gatcgccgcc cagaaccctt caagcaccag cgaagccagg
     1261 ttcatgtgga ccagcagcga cacggtgagc agcgctgctc cggccagatt ggtcaggtgc
     1321 agcacgaagg gattgggctc gtcattcacg gtcagatagg cataggcggc gatgatgcag
     1381 gcggtgccgg caaagccgac cagcgttgcc caatcgagcg cgatcatcgc gtggctccgt
     1441 cgagcagtgc ctcgaacacc ggaaagctgg tggcaatcgg gcgggtgtcg tccacttcta
     1501 ccccggcgcg gctggcaagg ccggccaccg ccatactcat cgcgatgcgg tgatcgagat
     1561 gggtggtaac cggtccgcca ccggcaagca gttcgccgcc ggtgccttcg atgatcaggt
     1621 cgtccccggt ttcgcgcacc cgcgccccgg ccagggccaa cgcttcggcc atggcggtga
     1681 tccggtcgct ttccttgacc cgcagttcct caaggccagt ggtcaccgtg gtgccctcgg
     1741 ccagcgccgc ggcgacgaac agcaccggga actcatcgat catgctcggc gcaacggcgg
     1801 gatcgaccgc gatccccttc agcgccgagt gcttcaccac cagatcggcc accggctcgc
     1861 cgccaacctc gcgcgcgttc acttcggtga tgtcgccgcc catctggcgc agcacgtgga
     1921 ccagcccggc gcgggtgggg ttgagcccga cgttctcgat ggtcagctcg ctgcccggca
     1981 cgatcagcgc cgcgaccatg aagaacgcag cggacgaggg atcgcccgga acctcgatcg
     2041 tctgggggct gagttcggcc tcgccatgga tccggatgat ccgctcgccg ttcacttcct
     2101 cgacgtccag ttgtgccccg aacccgcgca gcatccgttc ggaatggtcg cgggtgggaa
     2161 ccggttcgat cacggtggta atgcccggag tgttgagccc ggcg
//
LOCUS       DASUDV010000191         5305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1044614,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000191 DASUDV010000000
VERSION     DASUDV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044614"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="dmpG"
                     /locus_tag="VFV30_04035"
     CDS             <1..722
                     /gene="dmpG"
                     /locus_tag="VFV30_04035"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEX4847287.1"
                     /translation="DIGVRSVRVATHCTEADVSKQHIGIARDLGMDVSGFLMMSHMIE
                     PERLASQALLMESYGAQCVYVTDSGGALDMDGVSARFEAYDRVLKPETQRGMHAHHNL
                     SLGVANSIVAAQCGAVRIDASLTGMGAGAGNAPLEVFVAAADRKGWNHGCDVSLLMDA
                     AEDLVRPLQDRPVRVDRETLSLGYAGVYSSFLRHAEKASETYGIDTREILLELGRRKM
                     VGGQEDMIVDVALDMVKARNG"
     gene            715..2175
                     /locus_tag="VFV30_04040"
     CDS             715..2175
                     /locus_tag="VFV30_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4847288.1"
                     /translation="MAEISQHRPFPAADVPGWDFETDVAVIGFGATGACAAIEAADAG
                     ARVLLFERNSGSGGASGLSGGEIYIGGGGGTDAQRAAGFEDSTEDFTQYLKLAGGPCA
                     DEAKCELYGREALNHYAWLKAQGVPYRGNYLPGKHIEPTDDSTLIWSGSEAAAPFCDI
                     AKPAPRGHVIQHMGWGGGRPLVDVLEASARAKGVEVITDARAMALIRDGERIVGVVVR
                     IDNQPRFVKAAKGVVLATGGFVFNEAMRRKYCPDTFKLNDPIGDKDDGLGIELGLGAG
                     GDAIHMDQFFTTCPWTMPESHAYGVFVNTKGQRFINEDCYHGRVSRCAVDQPDGKVYL
                     LLDNAHFVQPMDFARMSIAGTGETWDEVEAELEMPAGTLSATMAVYNEHAREGRDPLF
                     DKRVPILTPLDQGPFIALELDFHTSYFSFFTLGGLKTSTDGEVLDRGGAPVPGLYAAG
                     RCTSGLPAWGHGYSSGLSLADCTFFGRQAGRKAAGG"
     gene            2168..2620
                     /locus_tag="VFV30_04045"
     CDS             2168..2620
                     /locus_tag="VFV30_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4847289.1"
                     /translation="MAERLAERLRALEDREAIRELIARYGPLADSGQADAVAALFAED
                     GIYAVGGMGEAKGRAAIAALISGPIHQSLMADGCAHVLTNPAITLDGDRATARCHSIV
                     FRHEDGEWAPVRVAANRWELARGGGGWQVIRRDNALLDGSAAARLLLS"
     gene            complement(2613..3353)
                     /locus_tag="VFV30_04050"
     CDS             complement(2613..3353)
                     /locus_tag="VFV30_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847290.1"
                     /translation="MRFAGKRVLVTGAASGIGRATAQLFAREGAQVIIADINEAGLAE
                     TAALIGEAATAVPYNAEDHASCRALVAKAAEGGLDVLCNVAGMLKWGPTETYAVEDFE
                     RLLRINTTSVFVLCQAALPHLVESKGNVVNIGSTSGLRGMIYTAAYCASKHAVVGMTT
                     ALAAEYAPKGVRINAVAPGHVNTPMTKQAPPEGDLDWGMVMRGMPKLVDGSCDPEDIA
                     EAVAFLASDKARKATGTVLVVDGGQLVS"
     gene            complement(3366..3863)
                     /locus_tag="VFV30_04055"
     CDS             complement(3366..3863)
                     /locus_tag="VFV30_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4847291.1"
                     /translation="MASPEERLQRLEDDRAIRDLKARYLRACDSKDPETVRDCLKPDG
                     AVIAYDGFPAFDSRDAFVAVYAEMGCAPGIFDIHHGANGVIEFDGSDRARGRWSLLFH
                     NINLASRTLTQMGVEYDDVYVKADGRWWIAETRSRRLSCLIHAVDAEGRPTVAAMGEA
                     PETFG"
     gene            complement(3869..>5305)
                     /locus_tag="VFV30_04060"
     CDS             complement(3869..>5305)
                     /locus_tag="VFV30_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847292.1"
                     /translation="NLYTNPNRATTAAIDAPAYERSRSYGHALTLTADFGPVTLKSIT
                     AYRDLDWADGLDLDGSPLPVAFTQRITDYHAFSQELQLTGEAGGGRLTYVLGGFYFDE
                     KAETLNPQTYFGGGVDLQSDYGSNTEAFALYAQLDYKLTDRVKISLGGRYTHEKKDVR
                     RYFRVNFDPANGIFTPLVVANLPYGVIPEATYNNFSPAVTVAWEASDNVNFYGRFARG
                     FKSGGFNGETNVFVAPTPACPSGATELCSPYRPEKVDSFELGMKTKLADGRVILNVAA
                     FRSEHKDMQLSVFTATTGAASIIRNAAGARIQGLEFETVLRPFESLTLNASLAILDPK
                     YKSFIDGGVDVSANRAFPHAPKTTASFGFDWEAVSGDWGKLNLYGDLSYVSKYYTFPY
                     PLVTPTLSDQNAHSSQSQGRTIINLRASLSDVSIGGAKAEVSVFVRNLTKEGNPSNFI
                     DFGPGFGGILLGYFPEPRTFGVTVGLKF"
BASE COUNT          846 a         1736 c         1841 g          882 t
ORIGIN      
        1 atgacatcgg cgtgcgctcg gtccgggtcg cgacccactg caccgaggcc gatgtttcga
       61 agcagcacat cgggattgcc cgcgatctgg gcatggacgt ctccggcttc ctgatgatga
      121 gccacatgat cgagccggag cgtctggcca gccaggccct gctgatggaa agctacggcg
      181 cgcagtgcgt ctatgtcacc gacagcggcg gggcgctcga catggacggg gtaagcgccc
      241 ggttcgaagc ctatgaccgg gtgctgaagc ccgaaaccca gcgcggcatg cacgcccacc
      301 acaacctttc gctcggcgtc gccaactcga tcgtcgcggc gcagtgcggc gcggtgcgga
      361 tcgatgccag cctgaccggg atgggcgcgg gcgcgggcaa tgccccgctt gaagtcttcg
      421 tcgctgcggc cgaccgcaag ggctggaacc acggctgcga cgtcagcctg ctgatggacg
      481 cggccgagga tctggtccgc ccgctgcagg accgcccggt gcgggtcgac cgcgaaacgc
      541 tcagcctcgg ctatgccggg gtctattcca gcttcctgcg ccacgccgaa aaggccagcg
      601 agacctacgg catcgacacg cgcgagatcc tgctggagct gggccgccgc aagatggtcg
      661 gcgggcagga agacatgatt gtcgacgttg ccctggatat ggtgaaggcc cgcaatggct
      721 gaaatttcgc agcaccgccc ttttcccgcc gccgacgtgc ccggctggga ttttgaaacc
      781 gacgtcgccg tgatcggctt tggcgcgact ggggcctgcg ccgcgatcga agcggccgat
      841 gccggagcgc gggttctgct gttcgagcgc aattcgggca gcggcggggc ttcgggcctg
      901 tcgggcggag agatctacat cggcgggggc ggcggcaccg atgcccagcg cgccgccggg
      961 ttcgaggact cgaccgagga cttcacgcaa tacctcaagc ttgctggagg tccctgcgcc
     1021 gacgaggcga agtgcgaact ctatggccgc gaggcgctga accactatgc ctggctcaag
     1081 gcgcaagggg tgccctatcg cggcaattac ctgcccggga agcacatcga gccgaccgac
     1141 gattccaccc tgatctggtc aggcagcgag gcggccgcgc cgttctgcga catcgccaag
     1201 ccggccccgc gcggccacgt gatccagcac atgggatggg gcggggggcg tccgctggtt
     1261 gacgtgctgg aagccagcgc gcgggccaag ggcgtcgagg tgatcaccga tgcccgcgcc
     1321 atggcgctga tccgcgatgg cgagcggatc gttggcgtgg tggtgcggat cgacaaccag
     1381 ccacgcttcg tcaaggcggc gaagggcgtg gtcctggcaa ccggcggctt cgtcttcaac
     1441 gaggcgatgc ggcgcaagta ctgccccgat acgttcaagc tcaacgatcc gatcggcgac
     1501 aaggacgatg gcctgggaat cgaactgggc ctgggcgcgg gcggcgacgc gatccacatg
     1561 gaccagttct ttaccacctg cccctggacc atgccggaaa gccacgctta cggcgttttc
     1621 gtcaacacca agggccagcg cttcatcaac gaggactgct atcacggccg ggtcagccgc
     1681 tgcgcggtcg atcagccgga cggcaaggtc tatctgctgc tcgacaacgc gcacttcgtc
     1741 cagccgatgg actttgcccg gatgagcatc gccggcacgg gcgagacctg ggacgaggtt
     1801 gaggccgagc tggagatgcc ggccggcacg ctcagcgcga ccatggcggt ttacaacgaa
     1861 cacgcccgcg aagggcgcga tccgctgttc gacaagcgcg tgccgatcct aaccccgctg
     1921 gaccaggggc cgttcatcgc gctcgagctc gatttccaca ccagctattt cagcttcttc
     1981 accctcggcg ggctcaagac ctcgaccgac ggcgaggtgc tggatcgggg cggggcgccg
     2041 gtgccggggc tctatgccgc cgggcgctgc acctcgggcc tgccggcctg ggggcacggc
     2101 tattcctcgg gcctcagcct ggccgattgc accttctttg gccgccaggc cggacgcaag
     2161 gcggccggtg gctgaacgcc tggccgaacg cctgcgggcg ctggaagacc gcgaggcaat
     2221 ccgcgagctg atcgcgcgct atggcccgct ggccgacagc ggccaggccg atgccgtggc
     2281 cgcactgttt gccgaggacg ggatctatgc cgtgggcggg atgggcgagg ccaaggggcg
     2341 tgctgccata gccgcgctga tcagcgggcc gatccaccag tcgctgatgg ccgatggctg
     2401 cgcccatgtg ctgaccaatc ccgcgatcac gctggacggc gaccgcgcca cggcgcgttg
     2461 ccacagcatc gtgttccggc acgaggacgg cgaatgggcc ccggtgcggg tggccgccaa
     2521 ccgctgggaa ctggcccgcg ggggcggggg ctggcaggtg atccggcggg ataacgccct
     2581 gctcgatggc agcgcggcgg cgcggctgct gctcagctga ccagctggcc gccgtccacc
     2641 accagcaccg tgccggtcgc cttgcgggcc ttgtccgagg cgaggaaggc caccgcttcg
     2701 gcaatgtctt cgggatcgca gctgccgtcg accagcttgg gcatcccgcg catcaccatg
     2761 ccccagtcca gatcgccctc gggcggggcc tgcttggtca tcggcgtgtt gacgtggccc
     2821 ggggcgaccg cgttgatccg cacgcccttg ggcgcatatt cggcggccag cgcggtcgtc
     2881 atgccgacca ccgcgtgctt cgaggcgcaa taggcggcgg tgtagatcat gccgcgcagg
     2941 cccgaagtgg atccgatgtt gaccacgttg cccttgcttt ccaccaggtg cggcagcgcc
     3001 gcctggcaca gcacgaagac gctggtggtg ttgatccgca gcagccgctc gaaatcctcg
     3061 accgcgtagg tttcggtcgg gccccatttc agcatgccgg ccacgttgca cagcacgtcg
     3121 agcccgcctt cggccgcctt ggcgaccagc gcgcggcagg aggcgtgatc ctcggcgttg
     3181 taggggacag ccgtggccgc ctccccgatc agcgcggcgg tctcggccag gcccgcctcg
     3241 ttgatatcgg cgatgatcac ctgggcgcct tcgcgggcga acagctgtgc ggtggcccgc
     3301 ccgatccccg atgccgcgcc ggtaaccagc acccgcttgc ctgcaaaccg cattccttgc
     3361 tctcctcagc cgaaagtttc gggcgcttcg cccattgccg ccacggtcgg ccggccctcc
     3421 gcatcgaccg cgtggatcag gcaggacagg cggcgcgagc gcgtttccgc gatccaccag
     3481 cggccgtccg ccttcacata gacgtcatcg tattccacgc ccatctgggt cagcgtgcgg
     3541 ctggccaggt tgatattgtg gaacagcagc gaccagcgcc cccgcgcccg gtcgctgcca
     3601 tcgaattcga tcaccccgtt ggcgccgtgg tggatatcga agatccccgg cgcgcagccc
     3661 atctcggcat agaccgcgac aaaggcgtcg cggctgtcga acgccgggaa gccgtcatag
     3721 gcaatgacgg ccccgtccgg cttcaggcaa tcgcggaccg tttccggatc cttgctgtcg
     3781 caggccctga gatagcgcgc cttgaggtcg cggattgccc ggtcgtcctc aaggcgctgc
     3841 agccgttcct ccgggctggc catccggctc agaacttcag cccgaccgtg accccgaagg
     3901 tgcgcggttc ggggaagtag cccagcagga tcccgccaaa gcccgggcca aagtcaatga
     3961 agttggacgg gttgccctcc ttcgtcaggt tgcggacgaa gaccgagact tcggccttgg
     4021 ccccgccgat cgaaacgtcg ctcaggctgg cccgcaggtt gatgatcgtg cgcccctgcg
     4081 actggctgga atgggcgttc tggtccgaca gggtcggcgt caccagcggg taggggaagg
     4141 tgtagtactt cgagacatag ctgagatcgc cgtagaggtt cagcttgccc cagtcgccgc
     4201 tcaccgcttc ccaatcgaac ccgaaggagg cggtcgtctt gggggcatgg gggaaggcgc
     4261 ggttggccga gacatccacg ccgccatcga tgaagctctt gtacttggga tcgaggatcg
     4321 ccagcgaggc gttgagcgtc aggctttcga acgggcgcag caccgtttcg aattccagcc
     4381 cctggatccg cgcgccggcg gcgttgcgga tgatcgaggc ggcgccggtg gtggcggtga
     4441 agaccgacag ctgcatgtcc ttgtgctcgc tgcggaaggc cgcgacgttg aggattaccc
     4501 ggccgtcggc cagcttggtc ttcatgccca gctcgaagct gtcgaccttt tccggccggt
     4561 agggcgagca cagctcggtc gcgccgctcg ggcaggccgg ggtcggggcg acgaagacgt
     4621 tggtttcccc gttgaagccg cccgacttga agccgcgggc aaagcggcca tagaagttga
     4681 cgttgtcgct ggcttcccag gccaccgtca cggccggcga gaagttgttg tacgtggcct
     4741 ccgggatcac gccatagggc agattggcga cgaccagcgg cgtgaagatg ccgttggccg
     4801 gatcgaaatt gacccggaag tagcgccgca cgtccttctt ctcgtgtgtg tagcggccgc
     4861 ccaggctgat cttcacccgg tcggtcagct tgtagtcgag ctgggcgtag agcgcgaagg
     4921 cttcggtgtt ggagccatag tccgactgca ggtcgacccc gccgccgaag taggtctgcg
     4981 ggttcagcgt ttcggccttc tcgtcgaagt agaacccgcc cagcacatag gtgagccgac
     5041 cgccgccggc ttccccggtc agctgcagtt cctggctgaa ggcatggtag tcggtgatcc
     5101 gctgcgtgaa ggcgaccggc aggggcgaac cgtcaaggtc caggccgtcc gcccagtcga
     5161 gatcgcggta ggcggtgatc gacttcagcg tgaccgggcc gaaatcggcg gtcagcgtca
     5221 gggcgtggcc gtaagagcgc gaccgttcat aggccggggc gtcgatcgcc gcggtcgttg
     5281 cccggttcgg gttggtgtag agatt
//
LOCUS       DASUDV010000192         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_271338,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000192 DASUDV010000000
VERSION     DASUDV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271338"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2192)
                     /locus_tag="VFV30_04065"
     CDS             complement(63..2192)
                     /locus_tag="VFV30_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847293.1"
                     /translation="MRLNNLDFRGRLDSASRIACAAALAWAGPALADPDPNTPPAEVP
                     AAAPVKAPGESRVKTYDAAYFAAFAPNTALDVVRRVPGFALEETEGEVRGFSQAAGNV
                     VINGARPSSKSESLGEILARIPARRVVRVEVAPGDLYGSDYAGKSQVLNLVLSDSGGL
                     DGNVKASASWIHTGWIVPNLEASVLIRTSGSTINLSAGTGRGGQVEVGYDDLTRTSDG
                     SRVELRDKVNTIEPRNPYFSASWGQEGAGGRAAHLNLRYVPGRFTLKQTNRVTPASGP
                     VRDDRLIQDYHPTSYEIGGDIARPLGPGVIKFVALANRRDRDAFDASYQRVGGVTQGG
                     FEANQTSLYDEVLGRISWSAPKLAGFSAELGSEVAYNKLDNATELFLLGPGGSRTRID
                     LPIDRAVVDELRTESYLSLGRQISGPLRLETRMAFETSALEVSGDTTAKRNLSYFKPS
                     VTLDWKGPKGWHAQLVARRTVAQLDFFDFLSSAELTNDRINGGNADLVPQRTWEFRLT
                     VDRPILGQGVVRLELGHDRVSRLQDRILTPEGFDAPGNIGSGTRSFIQLTIDAPLDRL
                     GLKATRIKFSGALQDHSVRDPLTGQDRPWSGFRPKWSWNAELRRDLSQWSYGVEVFRQ
                     DRNTVYRIDELDSFFNSGPFVLGFVEFRPDKRTTLRLDVENIADVAGQRERLFFDPNR
                     TSPQPVLREFRHRNAHVGVTVSVNRTF"
     gene            complement(2364..>2625)
                     /locus_tag="VFV30_04070"
     CDS             complement(2364..>2625)
                     /locus_tag="VFV30_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847294.1"
                     /translation="AALSPDSFGALVSLTFNRGPSYGKARSANDPLDRYREMRAIKAH
                     MAVGNYAAIPAEISAMQRIWPTVAGLRRRRREEAALFRDGLA"
BASE COUNT          398 a          909 c          894 g          424 t
ORIGIN      
        1 cccgcactcc ctcgtttgct gcgcggactg tgccgcgccg gcagcgcgaa gtcgaggccg
       61 ggctagaacg tccggttgac cgaaaccgtc accccgacat gggcgttgcg gtggcggaat
      121 tcgcgcagga ccggctgcgg gctggtgcgg ttgggatcga agaacagccg ctcgcgctgt
      181 ccagcgacgt cggcgatgtt ctccacgtcc agccgcagcg tggtgcgctt gtcggggcgg
      241 aattcgacaa agcccagcac gaacggcccg gagttgaaga agctgtccag ctcgtcgatc
      301 cggtagacgg tgttgcggtc ctgccggaac acctcgaccc cataggacca ttgtgaaagg
      361 tcgcggcgca gttcggcatt ccagctccac ttcggccgga agccgctcca cggccggtcc
      421 tgcccggtca gcggatcgcg caccgaatgg tcctgcaagg cgccagagaa cttgatccgg
      481 gtcgccttca ggcccagccg gtccagcggg gcgtcgatcg tcagctggat gaagctgcgc
      541 gtgccgctgc cgatgttgcc aggcgcgtcg aagccttcgg gcgtcaggat gcggtcctgc
      601 aggcgcgaga cgcggtcgtg gcccagttcc aaccggacca cgccctggcc caggatcggg
      661 cggtcgacgg tcaggcgaaa ttcccaggtc cgctgcggca ccagatcggc gttgccgccg
      721 ttgatccggt cgttggtcag ctcggcgctg gagaggaaat cgaagaaatc gagctgggcc
      781 acggtgcgcc gtgcgaccag ctgggcgtgc cagcccttcg gccccttcca gtccagcgtc
      841 acgctgggct tgaagtagct gaggttgcgc ttggcagtgg tgtcgcccga cacttccagc
      901 gccgaggttt caaaggccat ccgcgtttcc agccgcagcg gtccgctgat ctgccggccc
      961 aggctgaggt agctttcggt cctgagctcg tcgaccaccg cgcggtcgat cggcaggtcg
     1021 atccgggtgc ggctgccgcc cgggccaagc aggaacagct cggtcgcgtt gtcgagcttg
     1081 ttgtaggcca cttcgctgcc cagctcggcc gaaaaaccgg ccagtttcgg tgccgaccag
     1141 cttatccgcc ccagcacttc gtcatagagc gaggtctggt tcgcttcgaa cccgccctgg
     1201 gtcaccccgc cgacccgctg atagctggcg tcgaaggcat cgcggtcgcg gcggttggcc
     1261 agggcgacga acttgatcac ccccgggccg agcgggcggg caatatcgcc gccgatctca
     1321 taggacgtgg ggtgatagtc ctggatcagc cggtcgtcgc ggaccggccc gctggccggg
     1381 gtgacccggt tggtctgctt cagggtgaag cggccgggca catagcgcag attgaggtgc
     1441 gcggcgcgcc ccccagcccc ttcctggccc cagctggccg agaaataggg attgcgcggt
     1501 tcgatcgtgt tgaccttgtc gcgcagctcc acccgcgatc cgtccgaggt gcgggtcagg
     1561 tcgtcatagc cgacttccac ttgcccgccc cgcccggttc cggcggaaag gttgatggtc
     1621 gatccgctgg tgcggatcag caccgaggct tcgagattgg gcacgatcca gccggtgtgg
     1681 atccagctgg cgctcgcctt gacgttgccg tcaagcccgc cgctgtccga cagcaccagg
     1741 ttgagcacct ggctcttccc ggcatagtcc gacccgtaga ggtcgcccgg cgccacttcg
     1801 acccgcacca cccgccgggc cgggatccgg gcgaggatct cgcccagcga ttccgacttg
     1861 gacgagggcc gcgcgccgtt gatcaccacg ttgccggcgg cctggctgaa accgcgcact
     1921 tcgccctcgg tctcctccag cgcaaagccg gggacccggc gcaccacatc gagcgcggtg
     1981 ttgggggcaa aggcggcgaa ataggccgcg tcataggtct tcacccggct ttcgcccggg
     2041 gccttgacgg gcgccgccgc cggcacttcg gcgggcggcg tgttggggtc cggatcggcc
     2101 agggccggcc cggcccaggc aagggcggcg gcacaggcga tccggctggc cgaatccaat
     2161 ctgccccgaa aatcgaggtt attcaaacgc atgggactcc cctcccggca tcaatgtcgt
     2221 gccggccgga aatcccgtgg cgcgcttccc cagcgcgcta cgcagcccca tgcccgatcg
     2281 gccaagccat ccatgcccgc ccgcgcggtg tccaccaccg cggatcgacc aagcgcaatg
     2341 ccgggccgac caagtgcccc ggctcaggcc aggccatcgc ggaacagcgc cgcctcctcg
     2401 cgccggcggc ggcgcagccc ggccacggtc ggccagatcc gctgcatcgc ggaaatctcg
     2461 gccgggatcg cggcgtaatt gcccaccgcc atgtgcgcct tgatcgcccg catctcgcgg
     2521 taacggtcga gcggatcgtt ggcgctgcgg gccttgccat agctcggccc gcggttgaac
     2581 gtcagcgaga cgagcgcgcc aaagctgtcg ggcgagagcg ccgcg
//
LOCUS       DASUDV010000193         5506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1618642,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000193 DASUDV010000000
VERSION     DASUDV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618642"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFV30_04075"
     CDS             complement(<1..199)
                     /locus_tag="VFV30_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX4847295.1"
                     /translation="MRIALFEPEIAGNVGAVLRLGACLGVPVDLIEPMGFVWDDRRVR
                     RTAMDYIDHVDVTRHENFGAFC"
     gene            complement(202..840)
                     /locus_tag="VFV30_04080"
     CDS             complement(202..840)
                     /locus_tag="VFV30_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX4847296.1"
                     /translation="MKARSRLLALARERKVSLARLSELIGRNTTYLQQFVSRGSPRRL
                     EERDRRTLARFFGVGEDELGAPEGFSPGDSLAPRADWCEVPRLALGASAGPGAVPHEE
                     LPVGAFRFSARWLREQGLDPAQLSAIRVEGDSMEATLRDGDEILVDRTVRPLRDGIHV
                     VRTGDALLVKRLDLARPGTIALVSDNPAYRTIELPPEEVEVIGRVVWKSGRL"
     gene            974..1162
                     /locus_tag="VFV30_04085"
     CDS             974..1162
                     /locus_tag="VFV30_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847297.1"
                     /translation="MLITRIERFLRETGMPWTKFGRLAAHDPRFVQDLRNGRTPRDAT
                     AKRVEQFMNIYREARRAN"
     gene            1152..1523
                     /locus_tag="VFV30_04090"
     CDS             1152..1523
                     /locus_tag="VFV30_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847298.1"
                     /translation="MQIDLSLDQTSLSAARRAARHSPWLPLLSELLALAQGQAELIRH
                     AERNWASVTFSGTRHTVTLAFAGDEAVAAGEAFLTALPDHEFTVPRQLVADAAVVAVE
                     HTALPQPRLEVTAELLLLEDG"
     gene            complement(1532..2476)
                     /locus_tag="VFV30_04095"
     CDS             complement(1532..2476)
                     /locus_tag="VFV30_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX4847299.1"
                     /translation="MSTTVIVLNALLRPLIEPHLPEWAEVRWYASKDEAFDLAPAAEI
                     GWFDMHSKADMAEVFRRAERLKWLNSVYAGVEMFPLDVLAQRGVVLTNGAGINAAPIA
                     EYILLLMLAQCKGYREVVRAQDRHEWLKDSPGKRELLGSKALILGYGAIGQEVEKRLL
                     GMGMEVTKVRRHPGPGALGPDEWRGQLGTFDWIVLSVPATPETEGMIGAAELAAMKRD
                     AVLVNVARGSVIDQPALTAALAEGRIGGALLDVTTPEPLPADDPLWGLENTQITMHLS
                     GRAQDRMFQRSAARFLENLERYHRGEPLEPRVDYALGY"
     gene            2526..3245
                     /locus_tag="VFV30_04100"
     CDS             2526..3245
                     /locus_tag="VFV30_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847300.1"
                     /translation="MKKFVIAIGLLASALPAVSWSQQNAISEGNMEVIVTAQRRDAED
                     YSSEIPAIGLVRKADFAVVTVSVMGDSRDRLERQNEIYAMVRGAIEAAPRAGVELAYG
                     ARTLEPLTMANYKDLSLRNDSRPDSQRVTFLAKVPLAGTDAKTAEQRIATFIKSVKPV
                     GRALMERSGEMQLSIVAPDKYRGQVADLIIADAKAMGEKLGPDYSIQIEGLNRPVEWA
                     RAGLSEVMIYIPYRLVVGRKP"
     gene            complement(3242..3691)
                     /locus_tag="VFV30_04105"
     CDS             complement(3242..3691)
                     /locus_tag="VFV30_04105"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX4847301.1"
                     /translation="MKLWRLTRAPFVALDGKGAEINGARYAPEGVPVVSLASEAGLAV
                     LVALRYHLPDLSAAPDDLVLGWTTVDTVPERAPDHDEAATRAWVGEWLTSGRSLTCAI
                     RSKVLSEADVIYLNPRHPDAAAVPPLTVRPFSFAECLHRPPMRDRYG"
     gene            complement(3688..4053)
                     /locus_tag="VFV30_04110"
     CDS             complement(3688..4053)
                     /locus_tag="VFV30_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847302.1"
                     /translation="MLQPIAWGLLALVHLMPALAFFRPATLTTLYRIQPDNPLFLLMH
                     HRAALFVAVFAACLIAAFDPGSRKLASVITAISMLSFLWLWWQAGSPGALKTIAIVDL
                     IGLPVLAYAALGAFGQIGK"
     gene            complement(4090..5505)
                     /gene="cysS"
                     /locus_tag="VFV30_04115"
     CDS             complement(4090..5505)
                     /gene="cysS"
                     /locus_tag="VFV30_04115"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX4847303.1"
                     /translation="MTRQLEEFRPVHPGEARVSTCGPTVYNYQHIGNMRAYVFADTLG
                     RTLQFKGYKLTHVINITDVGHLTSDADAGEDKMEKMAAQAGKSAWDIAKFYQADFEAD
                     LARLNVEKKAHPRATEFVEAMIAWGKEIADKHCYELDSGLYFDVSTVPDYGRLARAVT
                     EDGEGRIETVEGKRNAADFAIWRKTPPGETRQMEWDSPWGRGAPGWHLECSVMGEALL
                     GMPFDIHTGGIDHREIHHPNEIAQNQAYCCTGGLSNAANSGARIWMHNNFLVERSGKM
                     SKSSGEFLRLQLLADKGYHPLAYRMMCLQAHYRSELEFSWDGLGAALTRLKRMVMAVA
                     NLRAHTEPAAAPGPRFAPFLERFEAAVSDDLNTSIALTVLEEVVGLKKVDAGEKLAAI
                     AAMDAVTGLGLLTLDRTDLRTRPKDAAITEAEIEATLAARKEARAAKDFAASDKLRDE
                     LAAQGVEVMDGDPLGWDWKLG"
BASE COUNT          923 a         1902 c         1778 g          903 t
ORIGIN      
        1 cgcaaaaggc accgaaattt tcgtgccggg tgacgtcgac atggtcgata tagtccatcg
       61 ccgtccgccg cacgcggcgg tcgtcccaga cgaagcccat cggctcgatc aggtccacgg
      121 ggacgcccag gcaggccccg agccgcagca cggcgccgac gttgccggcg atctcgggct
      181 cgaacagcgc gatccgcatg gtcacagccg cccgcttttc cacaccaccc ggccgatcac
      241 ctcgacctct tcgggcggca attcgatcgt gcggtaggcg ggattgtcgc tgaccagcgc
      301 aatggtgccg ggccgggcca ggtcgagccg cttgaccagc agggcatcgc cggtgcggac
      361 cacgtggatc ccgtcgcgca gcgggcggac cgtgcggtcg accaggatct cgtccccgtc
      421 gcgcagggtt gcttccatcg aatcgccttc gacccggatt gcggaaagct gcgcgggatc
      481 gagcccctgt tcgcgcagcc agcgcgccga aaagcgaaag gccccgaccg gcagttcctc
      541 gtgcggcacg gcccccggcc cggccgaggc ccccagcgcc aggcgcggca cctcgcacca
      601 gtcagcccgg ggggcaagcg aatcacccgg tgaaaatcct tcgggggcac ccagctcgtc
      661 ttcgccgacg ccaaagaacc gggccagggt gcggcggtcc cgctcttcca gccggcgcgg
      721 actgccccgg gaaacaaatt gctgtaaata tgtagtattt cttccgatca gttccgacag
      781 acgggccagg ctgactttcc gttcgcgcgc cagagccagt aaccgggacc gtgctttcat
      841 ctcggattcc gaagtctttt ccatgtttcg tagttaatcg atgtattttt cctagacaag
      901 taggatttcg cggggttcaa aaggaatatt cctacgtcga ttcgtccgcc acacagaaag
      961 ggggcacccc accatgctga ttacccggat cgaacgcttt ctgcgtgaaa ccggaatgcc
     1021 ctggaccaag ttcgggcggc ttgccgccca tgacccgcgc tttgtccagg acctgcgcaa
     1081 cggccgcact ccccgcgatg caaccgcgaa acgtgtggaa cagttcatga acatctatcg
     1141 cgaggcccgc cgtgcaaatt gatctttcgc tcgaccagac gtccctttcc gcggcccgcc
     1201 gggccgcccg gcactccccc tggctgccgc tcctgtccga attgcttgcc cttgcccagg
     1261 gccaggccga actgatccgc catgccgagc ggaactgggc cagcgtgacc ttttccggca
     1321 cccgccacac cgtgaccctg gcctttgccg gggacgaagc ggttgccgct ggcgaagcct
     1381 tcctcaccgc cctgcccgat cacgaattca ccgtgccgcg ccagctggtg gccgatgccg
     1441 cggtggtggc ggtggagcac accgccctgc cccagccgcg gctggaggtg accgccgaac
     1501 tgctgctgct ggaggacggc tagcgcgagg ctcagtaccc cagcgcgtaa tcgacgcgcg
     1561 gttccagcgg ctcgccgcgg tgataccgct ccaggttttc caggaaccgc gcggccgagc
     1621 gctggaacat ccggtcctgg gcccggcccg acaggtgcat ggtgatctgg gtgttctcca
     1681 gcccccacag cggatcgtcg gccggcagcg gctcgggcgt ggtcacgtcc agcagggccc
     1741 cgccgatccg cccttcggcc agcgccgcgg tcagcgcggg ctgatcgatc accgatccgc
     1801 gcgcgacatt caccagcacc gcgtcgcgct tcatcgccgc cagctcggcc gcgccgatca
     1861 tgccttcggt ttccggcgtg gccgggaccg acaggacgat ccagtcaaac gtgccaagct
     1921 gcccgcgcca ttcgtccggc cccagcgcgc ccggcccggg atgacggcgg accttggtca
     1981 cctccatccc catgcccagc aggcgctttt ccacctcttg cccgatcgcg ccatagccca
     2041 ggatcagcgc cttgctgccc agcaactcgc gcttgcccgg cgaatccttc agccattcgt
     2101 ggcggtcctg cgcgcgcacc acctcgcggt agcccttgca ctgcgccagc atcagcagaa
     2161 ggatgtattc ggcaatcggc gcggcgttga ttccggcgcc gttggtcagc accacgccgc
     2221 gctgggccaa cacgtcgagc gggaacatct ccaccccggc atagaccgaa ttgagccatt
     2281 tcagccgctc ggcgcggcgg aacacctcgg ccatgtcggc cttggaatgc atgtcgaacc
     2341 agccgatctc cgccgcaggg gccaggtcaa acgcctcgtc cttgctggca taccagcgca
     2401 cctcggccca ctccggcagg tgcggttcga tcaggggccg cagcagggcg ttgagcacga
     2461 taacggtcgt tgacatggcc tctccccggg cgcctactcg ctgcgccgcg tgaggagact
     2521 tagccatgaa aaagttcgtc attgcaatcg gattgctcgc ctcggccctg ccggccgtca
     2581 gctggagcca gcagaacgcc atcagcgaag gcaacatgga ggtgatcgtc accgcccagc
     2641 gccgcgacgc ggaagactat tcctcagaga tccccgcgat cgggctggtc cgcaaggccg
     2701 acttcgcggt ggtgaccgtg tcggtgatgg gcgacagccg cgaccggctg gaacgccaga
     2761 acgaaatcta cgcgatggtg cgcggcgcga tcgaggcggc cccgcgtgcc ggcgtggaac
     2821 tggcctacgg cgcgcggacg ctggagccgc tgaccatggc caattacaag gacctttcgc
     2881 tgcgcaacga cagccggccc gacagccagc gggtgacctt cctggccaag gtgccgctgg
     2941 ccggaaccga cgccaagacc gcggagcagc ggatcgcgac cttcatcaag agcgtgaagc
     3001 cggtcggccg ggcgctgatg gagcggagcg gcgagatgca gctgtcgatc gtcgcgccgg
     3061 acaagtaccg cgggcaggtt gccgatctga tcatcgccga tgccaaggcc atgggcgaaa
     3121 agctggggcc ggactattcg atccagatcg aagggctgaa ccgcccggtc gaatgggccc
     3181 gggccggact gtccgaagtg atgatctaca tcccctaccg cctggtcgtc ggccgcaagc
     3241 cctagccgta gcggtcccgc atcggcgggc ggtgcaggca ctcggcaaag gaaaacggcc
     3301 gcacggtgag cggcggcacc gcggcggcgt cggggtgccg cgggttgagg tagatcacgt
     3361 ccgcctcaga cagcaccttt gagcggatcg cgcaggtcag cgaacggccg ctggtcagcc
     3421 attcgccaac ccaggcgcgg gtcgccgcct cgtcatggtc cggcgcgcgt tcgggcacgg
     3481 tatcgaccgt ggtccagccc agcaccagat cgtccggcgc ggcggacagg tcgggcaagt
     3541 gatagcgcag cgccaccagc accgcgagcc cggcctcgct ggccaggctg accaccggca
     3601 ccccctccgg cgcataacgc gcgccgttta tctccgcgcc cttcccatcc aacgccacaa
     3661 acggcgcgcg ggtgaggcgc cagagtttca cttgccgatc tggccgaatg ccccgagagc
     3721 ggcgtaggcc agcaccggca atccgatcag atcgacaatg gcaatggtct tcagcgcgcc
     3781 gggcgaaccc gcctgccacc acagccacag gaacgagagc atcgagatcg ccgtgatcac
     3841 gctggccagc ttgcggctgc cgggatcgaa ggccgcgatc aggcaagccg caaacaccgc
     3901 cacgaacagc gcggcacggt ggtgcatcaa caggaacagc gggttgtccg gctggatccg
     3961 gtaaagcgtg gtcagagtgg caggccggaa aaaggccagg gccggcatca ggtggaccag
     4021 cgcgagcagg ccccaggcga tcggttgcag catactgtca ccctatcgcc gcgctgggga
     4081 ttggcaagtt cagcccagct tccagtccca gccgagcgga tcgccgtcca ttacctcgac
     4141 tccctgcgcg gcgagttcgt cgcgcagctt gtcggaggcg gcgaagtcct tggcggcgcg
     4201 ggcctccttg cgggcggcga gcgtggcttc gatctcggct tcggtgatcg cggcatcctt
     4261 gggccgggtg cgcaggtcgg tgcggtccag cgtcagcagt ccgaggcccg tgaccgcatc
     4321 cattgccgcg atcgccgcga gcttctcgcc ggcatcgacc tttttcaggc caaccacctc
     4381 ttccagcacc gtcagcgcga tcgaggtgtt gagatcgtcc gacaccgccg cttcgaagcg
     4441 ttcgaggaag ggggcgaagc gcggtcccgg tgcggccgca ggctcggtat gggcgcgaag
     4501 gttggcaacc gccatcacca tccgcttcaa ccgggtcagt gccgcgccca gcccgtccca
     4561 gctgaactcc agctcgctgc ggtagtgcgc ctgaaggcac atcatgcggt aggcgagcgg
     4621 gtgatagccc ttgtcggcca gcagttgcag ccgcaggaac tccccgctcg atttgctcat
     4681 cttgcccgaa cgttcgacca ggaagttgtt gtgcatccag atccgcgcgc cggaattggc
     4741 cgcgttgctg aggccgccgg tgcagcaata ggcctggttc tgggcgatct cgttcgggtg
     4801 gtgaatctcg cggtgatcga tcccgccggt atggatatcg aacggcatcc ccagcagggc
     4861 ctcgcccatg accgagcatt ccaggtgcca gcccggcgcg ccgcggcccc acggcgaatc
     4921 ccattccatc tggcgggttt cgcccggcgg ggtcttgcgc cagatcgcga agtcggcggc
     4981 gttgcgcttg ccctcgaccg tttcgatccg cccttccccg tcctcggtca cggcgcgggc
     5041 caggcggccg tagtcgggca cggtcgagac atcgaaatag agcccgctgt cgagctcgta
     5101 gcagtgcttg tcggcgattt ccttgcccca ggcgatcatc gcctcgacaa attcggtcgc
     5161 gcgcggatgg gccttcttct cgacattcag gcgggcgagg tcagcctcga aatcggcctg
     5221 atagaatttg gcgatgtccc aggcgctctt gcccgcctgg gccgccatct tctccatctt
     5281 gtcctcgccg gcgtcggcat cggaggtcag gtggccaaca tcggtgatgt tgatcacgtg
     5341 ggtgagcttg tagcccttga actgcagcgt gcgcccgagc gtgtcggcaa agacataggc
     5401 ccgcatgttg ccgatgtgct ggtaattgta gaccgtcggc ccgcaggtag agacgcgcgc
     5461 ctcacccgga tggacgggcc ggaactcttc cagctggcgg gtcagg
//
LOCUS       DASUDV010000194         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1154860,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000194 DASUDV010000000
VERSION     DASUDV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154860"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFV30_04120"
     CDS             <1..218
                     /locus_tag="VFV30_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX4847304.1"
                     /translation="GYFAPDGNLDSCIVLRTGVVKDGVLHVQAGAGIVADSDPAYEQR
                     ECEAKAGALIAAAREAVRVAGEAGFGQ"
     gene            215..604
                     /locus_tag="VFV30_04125"
     CDS             215..604
                     /locus_tag="VFV30_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX4847305.1"
                     /translation="MTASWEELESFSFGDNPEMADRLLGFVLAGSKRATCWSVADGQQ
                     THVGKRMVVLDGMGRPRAVIETVELEQRRFCDVDLQFALDEGEGDETLDDWRGGHQAY
                     FERNGGFDPEMMLWCERFALIAAIDPA"
     gene            615..1466
                     /locus_tag="VFV30_04130"
     CDS             615..1466
                     /locus_tag="VFV30_04130"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4847306.1"
                     /translation="MADTKVALVTGCSTGVGLHTAVRLAEGGYTVIATMRDPAKQQPL
                     LNEAAKAGVAVEVAPLDVCEQSSIDAAVSDAIARHGRIDLLVNNAGAGFLGSVEQTSD
                     DDLRRTMETNFFGVWNLTRAVLPHMRGAGRGRIISVTSVGGLLGQPFNEAYCAAKFAV
                     EGMMEGLAPLAKQFGVDICLVEPGPINTEFVSSVRATSQGAFAAMAEPYATLAQRYMA
                     ASGNVFATYGQTGDDIARIIAALAAAPQPDFRTITSDFAMATVASKVVDRTGNSVIQI
                     FADRLKG"
     gene            1470..2537
                     /locus_tag="VFV30_04135"
     CDS             1470..2537
                     /locus_tag="VFV30_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4847307.1"
                     /translation="MRKLLLLIAALIATPLHAEDWSKRASERDVVLKDFTYRSGEKAD
                     LRMHVTTLGTPRRNAKGEIENAVMILHGTGGSGHQFFRPQFADELYGPGQVLDITKWY
                     VILPDNIGHGKSSKPSDGLRMAFPRYGYFDMVDAQKRLLTEGLGVTKLQLILGTSMGC
                     MHSFVWGTEYPGFALRLAPFACNAMEIAGRNRMWRKMSIDAIKADPQWNGGNYTSPPE
                     SGLRTARYLGMIAGGNPVALQAQFPTREGAEKALADGYAARAGGDADANDQIYQLDSS
                     RDYNPLPKLRAITVPVLWINSADDFINPPELALAQQHAKLMPRVRFILIPATPETKGH
                     GTHTWAKFWKKDLAKLMATRP"
     gene            2534..3493
                     /gene="pip"
                     /locus_tag="VFV30_04140"
     CDS             2534..3493
                     /gene="pip"
                     /locus_tag="VFV30_04140"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX4847308.1"
                     /translation="MSERRTLYPPIEPYASGMLDVGDGHTLYWERVGTPGAKPAVMLH
                     GGPGGGCSDGHRRQWNPELYDVLLFDQRGCGRSTPFASLDANTTWHLVADIERLREMC
                     GHAEWQVFGGSWGSTLALSYAVTHPERVTELILRGIFLSSRKELEWLTRYGASELYPE
                     EWDRFVAHVDGKNGDDHTDAYWALLDSPDADVRRAAAAAWNRWEGVTCTLLPAPDTVD
                     HMADGDNAVATARIENHYFRHSCWLAEGELLSKVDRIRHIPGIIVQGRHDCCTPPTAA
                     WALKKAWPEAELQIVPDGGHLYTEPGITDGLVRATDRFAGKVD"
     gene            3490..3840
                     /locus_tag="VFV30_04145"
     CDS             3490..3840
                     /locus_tag="VFV30_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847309.1"
                     /translation="MKNIQIIDGAVNATFSLFQATDSEFAAIFPGGQDMELIEDLVTR
                     LGEEKASAILLPIWERPILKTEAQGIHGTIFYDNEARRDHLPASKREVDWPDGAINAA
                     QRALFAQRRAAERL"
     gene            3911..>4310
                     /locus_tag="VFV30_04150"
     CDS             3911..>4310
                     /locus_tag="VFV30_04150"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053647.1"
                     /note="aminodeoxychorismate synthase subunit PabA; with
                     PabB catalyzes the formation of 4-amino-4-deoxychorismate
                     from chorismate and glutamine in para-aminobenzoate
                     synthesis; PabA provides the glutamine amidotransferase
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX4847310.1"
                     /translation="MADILVIDNYDSFTWNLVHYLMELGAAVDVVRNDALSAGQAVTS
                     GAKGFLISPGPCTPNEAGISLDLVGACADAALPLLGVCLGHQSIGQHFGGRVVRGGLM
                     HGKTSPVTHDGSGLFAGLPSPFVATRYHSLV"
BASE COUNT          749 a         1442 c         1435 g          684 t
ORIGIN      
        1 tcggctattt cgcgcccgac ggcaacctcg acagctgcat cgtgctgcgc accggggtgg
       61 tcaaggacgg ggtgctgcac gtccaggccg gggccggcat cgttgccgac agcgatcccg
      121 cctatgaaca gcgcgaatgc gaggccaagg ccggggcgct gatcgccgcc gcccgcgaag
      181 ccgtgcgcgt ggcgggcgag gccgggttcg gtcagtgacc gcgtcctggg aagagctgga
      241 gagcttttcc ttcggcgaca atccggagat ggccgatcgt ttgctggggt tcgtcctggc
      301 cggaagcaag cgcgcgacct gctggtcggt ggccgatggc cagcaaaccc atgtcggcaa
      361 gcggatggtg gtgcttgacg gcatgggccg tccgcgggcg gtgatcgaga cggtcgagct
      421 ggaacagcgc cggttctgcg atgtcgacct gcagttcgcg ctcgacgagg gcgaaggcga
      481 cgagacgctg gacgactggc ggggcgggca ccaggcctat ttcgaacgaa acggtggctt
      541 cgatccggaa atgatgctgt ggtgtgaacg cttcgccctg atcgcggcga tcgacccggc
      601 ataggagaac cagcatggcc gatacgaagg tagcactggt aacgggctgc tccaccgggg
      661 tcggtctgca caccgcggtc cgcctggccg aaggcggcta caccgtgatc gccacgatgc
      721 gtgacccggc caagcagcag cccctgctga acgaagcggc caaggccggc gttgcggtgg
      781 aggtggcccc gctggatgtc tgcgaacaga gctctatcga tgccgccgtt tccgatgcga
      841 tcgcgcggca cgggcggatc gatctgctgg tcaacaacgc cggggcgggt tttctcggct
      901 cggtcgagca gaccagcgat gacgacctgc ggcggaccat ggaaacgaac ttcttcgggg
      961 tctggaacct gaccagggcg gtgctgccgc acatgcgcgg cgcgggccgc gggcggatca
     1021 tttccgtcac cagcgtcggc ggcctgctcg gccagccgtt caacgaggcc tattgcgccg
     1081 ccaagttcgc ggtcgaaggg atgatggagg ggctggcccc gctggccaag cagttcggcg
     1141 tcgatatctg cctggtcgag cccgggccga tcaacaccga attcgtctct tcggtccgcg
     1201 ccacttcgca gggcgcgttt gcggcgatgg ccgaacccta tgccaccctg gcccagcgct
     1261 atatggcggc ctcgggcaat gtctttgcca cctatggcca gaccggggac gacatcgccc
     1321 ggatcattgc cgcgctggcc gcggccccgc agccggactt tcgcaccatc acttcggact
     1381 tcgccatggc gactgtcgcg agcaaggtgg tcgaccgcac cggcaattcc gtcatccaga
     1441 tctttgccga ccgtctcaag ggataaccga tgcgcaagct gctgctcctg atcgccgccc
     1501 tgattgccac cccgctccac gccgaggact ggtccaaacg cgccagcgag cgggatgtgg
     1561 tcttgaagga cttcacttac cgcagcggcg agaaggccga cctccggatg cacgtgacca
     1621 cgctcggcac cccgcgccgc aacgccaagg gcgagatcga gaacgcggtg atgatcctgc
     1681 acggcaccgg cggatcgggc caccagttct tccgcccgca gttcgcggac gagctctacg
     1741 gccccggcca ggtgctcgac atcaccaagt ggtacgtgat cctgccggac aacatcggcc
     1801 acggcaaaag cagcaagccc agcgacgggc tgcgcatggc cttcccgcgc tatggctatt
     1861 tcgacatggt cgacgcgcag aagcggctgc tgaccgaggg cctgggcgtg accaagctgc
     1921 agctgatcct cggcacatcg atgggctgca tgcacagctt cgtctggggc accgagtacc
     1981 cgggctttgc gctgcggctg gcgccgtttg cctgcaacgc gatggaaatc gccgggcgca
     2041 accggatgtg gcgcaagatg tcgatcgacg cgatcaaggc cgatccgcag tggaacggcg
     2101 ggaactacac cagcccgccc gaaagcggcc tgcgcaccgc gcgctacctg gggatgatcg
     2161 ccggcggcaa cccggtggcg ctgcaggccc agttcccgac ccgtgagggc gcggaaaagg
     2221 cgctggccga tggctatgcc gcgcgcgccg ggggcgatgc cgatgccaac gaccagatct
     2281 atcagctgga tagctcgcgc gattacaacc cgctgcccaa gctgcgcgcc attaccgtgc
     2341 cggtgctgtg gatcaattcg gcggacgatt tcatcaaccc gcccgagctg gccctggccc
     2401 agcagcacgc caagctgatg ccgcgggtgc gcttcatcct gattccggcc accccggaaa
     2461 ccaagggcca cggcacccac acctgggcca agttctggaa gaaggacctg gccaagctga
     2521 tggcgacccg gccgtgagcg agcgccgcac gctctacccg ccgatcgagc cctatgcgag
     2581 cggcatgctc gatgtgggtg acggacatac gctgtactgg gaacgcgtcg gcacgcccgg
     2641 tgcaaaaccg gccgtgatgc ttcacggcgg cccgggcggc ggctgcagcg acgggcaccg
     2701 ccggcaatgg aacccggagc tctacgacgt cctgctgttc gaccagcgcg gctgcgggcg
     2761 ttcgacgcca ttcgccagcc ttgacgccaa cacgacctgg cacctcgtgg ccgatatcga
     2821 gcggctgcgg gagatgtgcg ggcacgctga gtggcaggtg ttcggcggca gctggggatc
     2881 gaccctggcg ctgtcctacg ccgtcaccca tcccgagcgc gtcaccgaac tgatcctgcg
     2941 cggcatcttc ctgtcgagcc gcaaggagct cgaatggctg acccgctacg gcgccagcga
     3001 gctttacccc gaagagtggg accgcttcgt ggcccacgtt gatggcaaga acggcgacga
     3061 ccacacggat gcctattggg ccctgctcga cagccccgat gccgatgtcc gccgcgccgc
     3121 cgcggcggcc tggaaccgtt gggaaggcgt gacctgcacc ctgctgcccg ctccggacac
     3181 ggtcgatcac atggccgacg gcgacaacgc ggtcgccacg gcgcggatcg agaaccacta
     3241 tttccgccat tcctgctggc tggccgaggg cgaactgctc tcaaaagtcg accgcatccg
     3301 gcacatcccc gggatcatcg tccagggccg tcacgactgc tgcacgcccc cgacggcggc
     3361 ctgggcgctg aagaaggcct ggccagaggc cgagctgcag atcgtgccgg acggcggcca
     3421 cctctacacc gaacccggca tcaccgacgg actggtccgg gcgaccgacc ggtttgcggg
     3481 caaggtcgat tgaaaaacat ccagatcatc gatggcgcgg tgaacgctac cttcagcctc
     3541 tttcaggcga ccgattccga attcgccgcg atcttccccg gtggtcagga catggaactg
     3601 attgaggatc tggttacccg ccttggcgag gagaaagcgt ccgcaatctt gctcccgatc
     3661 tgggaacgcc cgatcctgaa gaccgaagcg cagggaatcc acggcacgat cttttacgac
     3721 aacgaggcaa ggcgtgacca tcttcccgcg agcaagcgtg aagtcgattg gccggacggc
     3781 gcaatcaatg ccgcccagcg cgcgctcttt gcgcaacggc gagcggcaga gcggctctga
     3841 aggacccgct ttccccgcat caagcaagct tatctggcaa atcgccgccg acgcgcctat
     3901 gtgccccgcc atggccgata tccttgtcat cgacaattac gacagcttca cctggaacct
     3961 ggtccactac ctgatggaac tgggcgcggc ggtggatgtt gtgcgcaacg atgccctgtc
     4021 ggccgggcag gcggtgacca gcggggccaa gggtttcctg atctcgcccg ggccctgcac
     4081 ccccaacgag gccgggatca gcctcgatct ggtcggcgcc tgcgccgatg ccgccctgcc
     4141 gctgctgggc gtgtgcctgg ggcaccaatc gatcggccag catttcggcg ggcgcgtggt
     4201 ccgcggcggg ttaatgcacg gcaagaccag cccggtgacg cacgacggca gcgggctgtt
     4261 cgcggggctg ccctcgccct tcgtcgccac ccgctaccac tcgctggtgg
//
LOCUS       DASUDV010000195         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_690942,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000195 DASUDV010000000
VERSION     DASUDV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690942"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VFV30_04155"
     CDS             complement(<1..978)
                     /locus_tag="VFV30_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847311.1"
                     /translation="MTDAVAGQRPANSLRLTLWVLLIVYIFNFLDRQIVNILAEPIAR
                     DLDLKDWQIGVMTGIAFAAFYTFLGIPIARFADKPTTNRVGLISISLVVWSAMTALCG
                     MAQNFVQLLLARIGVGVGEAGCTPAAHSLIADLAPPEKRASAISFYSLGIPIGGLLGM
                     VMGGLVADAYGWRMAFLIAGLPGILMALVVWYVLRDPRMSAAVATRQAAPTMSTKEAL
                     GVIFKSRAFIYIAIAGAAAAFLGYGKGTWTTIFFQRTHDMTPGEVGFWFGIGGGIAGA
                     IGTFGGGWLADRFGKTNRRHVLTAPAIGMVVAIPIAIAAYWVQNWWVALL"
     gene            complement(1067..2083)
                     /locus_tag="VFV30_04160"
     CDS             complement(1067..2083)
                     /locus_tag="VFV30_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX4847312.1"
                     /translation="MSIDRPDPAISEAEAEARMAARRDDALATMAKADHRMLAGDYRA
                     AAAFYGAVGRLAGQGAAIPRGELLRARDAAVWLDNRFGEMLREGLEGAGLGEGQRHPR
                     FQLALDIMAGRRARAAEHDPFPQRPQTYYHPGLPLVHFADTATFAWREAVEAATPAIL
                     AEAQALLAEDGSFRPYMQQTRDRPQGDVHGLLDNPDWSTYYLTDKGQPIADRVARCPT
                     SYTVLDEGVPLCRIGTRAPSIMFSLLRPHSRIPAHTGMVNCRFICHLPLIVPPGCGFR
                     VAAETREWEVGRLLAFDDSVEHEAWNDSDQDRLVLIFDVWRPDVSLDERHQIETLFRI
                     VDSY"
     gene            complement(2085..>3214)
                     /locus_tag="VFV30_04165"
     CDS             complement(2085..>3214)
                     /locus_tag="VFV30_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX4847313.1"
                     /translation="ALVAHPASVTEDLTHSLDALIAAGVNVSAAFGPQHGIKGDKQDN
                     MVETEDELDPVYNIPVFSLYGEVRRPTGQMMSTADVFLFDLQDLGCRIYTFVTTLLYL
                     LEAAHGTGKEVWVLDRPNPAGRPIEGTLLVPGQESFVGAGPMPMRHGLTLGEMGHWFI
                     DHFKLDVPYRVIAMQGWEPEGPGHGWPPERIWINPSPNAASLNMGRAYAGTVLLEGAT
                     LSEGRGTTRPLEVLFGAPDVDAKAVLAEMRRFAPEWLAGCALRECWFTPTFHKHAGAL
                     CNGLMIHAEGRFYDHHAFRPWRLQALAFKAIRRLYPDYDLWRDFPYEYEFERLAIDVI
                     NGGPALREWVDDPAATPADLEAKAGADEAAWAAAVKPFLLY"
BASE COUNT          551 a         1092 c         1085 g          486 t
ORIGIN      
        1 cagcagcgcc acccaccagt tctgcaccca ataggccgcg atcgcgatgg ggatcgccac
       61 gaccatgccg atggccggcg cggtcagcac gtggcggcgg ttggtcttgc cgaagcggtc
      121 cgccagccag ccaccgccga aagtgccgat cgcccccgcg atgccgccgc cgatgccgaa
      181 ccagaagccg acttcgcccg gcgtcatgtc gtgcgtgcgc tggaagaaga tcgtggtcca
      241 ggtgcccttg ccatagccca ggaacgccgc cgcggccccg gcaatggcga tatagatgaa
      301 cgcgcgcgac ttgaagatca cgcccagcgc ttccttggtc gacatggtcg gcgcggcttg
      361 gcgggtggcg acggctgccg acatccgcgg atcgcgcagc acgtaccaca ccacaagcgc
      421 catcaggatg ccgggcaaac cggcgatcag gaaggccatg cgccagccat aggcatcggc
      481 caccaggccg cccatgacca tgccgagcag cccgccgatc gggatgccga gcgaatagaa
      541 cgagatcgcc gaggcgcgct tttccggcgg ggccaggtct gcaattagcg agtgcgcggc
      601 aggcgtgcag ccggcctcgc ccacccccac cccgatccgg gcgagcagca gctggacgaa
      661 gttctgggcc atgccgcaca gcgcggtcat cgccgaccag accaccagcg agatcgagat
      721 cagcccgacc cggttggtgg tcggcttgtc ggcaaagcgc gcgatcggaa tgccgaggaa
      781 ggtgtagaac gcggcaaagg caatgccggt catcaccccg atctgccagt ctttcaaatc
      841 caggtcgcgc gcgatcggct ccgccaggat gttgacgatc tgccggtcaa gaaagttgaa
      901 gatatagacg atcagcaaga cccacagggt caggcgcagg gaattcgcag ggcgttgccc
      961 tgcaacagca tcggtcatcg accatcctct cattgcccgg cgtcttgcgc gccgcgaatc
     1021 cctccatcgc tagggtggag ggcgcggcag gcccggtcaa gcgcggctag taggaatcga
     1081 cgatccggaa cagcgtttcg atctggtgcc gctcgtccag cgagacgtcg ggccgccaga
     1141 catcgaagat cagcaccaga cgatcctgat cgctgtcgtt ccaggcctcg tgctccaccg
     1201 aatcgtcgaa ggccagcaat ctgccgacct cccattcgcg cgtttccgcg gcgaccctaa
     1261 acccgcagcc cggcgggacg atcagcggca ggtggcagat gaagcggcaa ttgaccatgc
     1321 cggtgtgggc gggaatgcgc gaatgggggc gcagaagcga gaacatgatc gacggcgccc
     1381 gggtgccaat ccggcacagc ggcacgcctt catccagcac ggtgtaactg gttgggcacc
     1441 gggccacccg gtcggcaatc ggctgaccct tgtcggtcag gtaataggtg ctccagtccg
     1501 ggttatcgag caggccgtgg acgtcgccct gcggccggtc gcgggtctgc tgcatatagg
     1561 gacggaacga gccgtcttcg gccagcaggg cctgcgcttc ggccaggatt gccggggttg
     1621 ccgcctccac cgcctcgcgc caggcaaagg ttgccgtgtc cgcgaaatgg accagcggca
     1681 ggccggggtg gtaataggtt tgcgggcgct gcggaaaggg atcgtgttcc gcggcgcggg
     1741 cgcggcgccc ggccatgatg tcgagcgcca gctggaaccg cgggtgccgc tgcccctcgc
     1801 ccaacccggc cccctccagc ccttcgcgca gcatctcgcc aaagcggttg tcgagccaga
     1861 ccgccgcatc gcgggcgcgc aaaagttcgc cacgggggat cgccgcgccc tgccccgcca
     1921 gccgcccgac cgccccatag aaggcggcgg ccgcgcggta atctcccgcc agcatgcggt
     1981 gatcggcctt ggccatggtg gccagcgcat cgtcgcggcg cgcggccatc cgggcctcgg
     2041 cctcggcctc gctgatcgcg ggatcgggcc ggtcgatgct catctcagta gagcaggaac
     2101 ggctttaccg ccgcggccca ggcggcctcg tcggctcccg ccttggcctc gagatcggcc
     2161 ggcgtggcgg ccggatcgtc gacccattcg cgcaaggccg ggccgccgtt gatcacgtcg
     2221 atcgccagcc gctcgaattc gtactcatag gggaaatcgc gccacagatc gtagtcggga
     2281 tagagccgcc ggatcgcctt gaaggccaag gcctggagcc gccacggacg gaaggcgtga
     2341 tgatcgtaga accggccctc ggcgtggatc atcagcccgt tgcacagcgc gccggcgtgt
     2401 ttgtgaaagg tcggcgtgaa ccagcattcc cgcagagcgc agccggccag ccactcgggc
     2461 gcgaagcggc gcatttcggc cagaacggcc ttggcatcga catcgggcgc gccgaacagc
     2521 acttccagcg ggcgggtcgt gccccgccct tcgctcaggg tcgcgccttc cagcaggacc
     2581 gtgccagcat aggcacggcc catgttgagg ctggccgcgt tgggactggg gttgatccag
     2641 atccgctccg gcggccaccc gtggccgggt ccttcgggct cccacccctg catggcaatc
     2701 acgcggtaag gcacgtcgag cttgaagtgg tcgatgaacc agtgtcccat ttcacccagc
     2761 gtcagcccgt ggcgcatcgg catcggcccg gccccgacga agctttcctg ccccggcacc
     2821 agcagcgtgc cttcgatcgg gcggccggcg gggttcggcc ggtccagcac ccagacctcc
     2881 ttgccggtgc cgtgcgcggc ctccagcagg tagagcagcg tggtgacgaa ggtgtagatc
     2941 cggcagccca ggtcctgcag gtcaaacagg aacacgtcgg ccgtgctcat catctgtccg
     3001 gtcgggcggc ggacctcgcc gtaaaggctg aagaccggga tgttgtagac cgggtcgagt
     3061 tcgtcctcgg tctcgaccat gttgtcctgc ttgtcgccct tgataccatg ctgcgggccg
     3121 aaggcggcgc tgacgttgac gccggcggcg atcagcgcgt cgagcgaatg ggtcaggtcc
     3181 tcggtcaccg aggccggatg cgcgaccagc gcga
//
LOCUS       DASUDV010000196         4424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_514584,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000196 DASUDV010000000
VERSION     DASUDV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514584"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFV30_04170"
     CDS             complement(<1..205)
                     /locus_tag="VFV30_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4847314.1"
                     /translation="MSANIAEMEKRRAAARMGGGQKRIDAQHAKGKLTARERLSILLD
                     EGSFEETDMYVEHNCVDFGMPDTT"
     gene            316..1716
                     /locus_tag="VFV30_04175"
     CDS             316..1716
                     /locus_tag="VFV30_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX4847315.1"
                     /translation="MSPRRIFAGSRLRAIRTERRLKQSELAAALAISTSYLSQLENDD
                     RPLTPALVERLARAFPLDWRDIGAGDAETRLAALREANADPLFAEPMDAAHLARFAEQ
                     QPALAERFIALHEAYRRAGQRLAMVDEALSAETGSIARLPWEEVRDWFHLENNYVDSL
                     DRAAEALAGRLGALSPETGAIEAYLKDALSVSLIYTGLQGLRSYDAEMGHLTVDPGQP
                     PESRRFQLAHQLAALALKDEIAAVVENARLNSAASRQLLFVGLCNYAAGALLMPYRRF
                     RAEARSVRHDIDRLAQIFGTSFEQTCHRLSTLQREDAKGVPFFFCRVDMAGNITKRHS
                     ATRLQFARFGGACPLWIVHEAVAIPDRILVQLAETPDGVRYVSMAKGLVKPSGSYQRA
                     PRRYAVALGCEAEHANEFVYADGLDLKAAPAATRIGISCRICPRADCDQRAFPPSDRP
                     ISVDPDSRGIVPYRVG"
     gene            complement(1713..2729)
                     /locus_tag="VFV30_04180"
     CDS             complement(1713..2729)
                     /locus_tag="VFV30_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4847316.1"
                     /translation="MAGTVFVSGGSGYIAGFLIRQLIAEGWTVHTTIRNLAREAEVRE
                     SLGVDNAKLKFFAADLMSDAGWAEAMAGCSHVAHVASPLPADKPLHEDELIVPARDGA
                     LRALRAAKAAGIKRFVMTSSMAAIAYGHGGGKSVFTEADWTDPTSPDAYPYVRSKTIA
                     ERAARDWVAAEGGDMEFCTVNPSLVLGPLQSGDFSTSLEAIKKLLEGSMPGMPNLGFG
                     IVDVRDVADMHVRCLTAPNMAGERFICSGPFLMLDEVAAILRAGLGPQARKVPKLKLP
                     NWLVRIAARFDPVISQVIGELGKRRESPANHARDVLGWVPRPVEESVLDTARDMIRLG
                     IVKV"
     gene            complement(2743..3465)
                     /locus_tag="VFV30_04185"
     CDS             complement(2743..3465)
                     /locus_tag="VFV30_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847317.1"
                     /translation="MQLAGKTVLVTGATDGIGAHLIRQLRDKGATVIASGRNPDRIAA
                     TRADGFEVIAADLSGPEGVEALLAGLAGRPIDVLVNNAGVGTSYDFRSGEVDLAGAEK
                     TYFLNLHAPTQLIARLLPVLKARGEAMIVNVTSGLALAPNAGSPVYCASKAGLRSYTL
                     AVRAQLKGTGITVVEALPPLVDTQMTAGRGSNKMPPAECARQIVAGMERGTETINVGM
                     VKLLQVVHSISPALARSIMIRF"
     gene            complement(3524..>4424)
                     /locus_tag="VFV30_04190"
     CDS             complement(3524..>4424)
                     /locus_tag="VFV30_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4847318.1"
                     /translation="DGAEHCITSNEVFHLPELPKRIVIQGAGYIALEFAGVFNALGSQ
                     VTVVNRSDKILRGYDHDLTERLLPILQSRGIEFGFNRPIRKVEKVHDGSFLVHAGEGA
                     PIAADAVMVATGRKPKTEGLGLENAGIALGANGEVPVDEYNQTSCPSIYAVGDVTDRV
                     QLTPVAIREGHAFADTVFGGTPRTTAYDHIPSAVFTQPPIAAVGLTEEQARLVHGHVK
                     VFKSDFRPMQNMFSAVAERGYYKLVVDPASDVVLGVHMLGPDAPEILQAAAIAVKAGL
                     TKAQFDATVALHPSMAEELVLMR"
BASE COUNT          686 a         1550 c         1455 g          733 t
ORIGIN      
        1 tggtcgtgtc cggcatgccg aaatcgacgc agttgtgttc gacatacatg tcggtttctt
       61 caaagctgcc ctcgtccagc aggatcgaga gacgttcgcg cgcggtcagc ttgcccttgg
      121 cgtgctgtgc gtcgatccgc ttttgccccc cgcccatccg ggcggcggcg cggcgctttt
      181 ccatttcggc gatatttgcg gacattgagg ccccgtcaga aattgcgaag gctcgggcat
      241 ggaacccagg ggcggcaatg tccaatgtga atttgcaaag ttgcgaagcg gcactttgca
      301 aagtaggaga ggtccatgtc cccgcgccgc atctttgccg gaagccggct ccgcgcaatc
      361 cgcaccgagc gccggctcaa gcagtcggag ctggccgccg cgctggcgat cagcacctcc
      421 tacctctcgc agctggagaa cgacgaccgc cccctcaccc cggcgctggt cgagcggctg
      481 gcgcgggcct tcccgctcga ttggcgcgac atcggcgcag gcgatgcgga aacgcggctg
      541 gcggcgctgc gcgaggccaa tgccgatccg ctgttcgccg aaccgatgga cgccgcccac
      601 ctcgcccgct ttgccgagca gcagccggcc ctggccgagc ggttcattgc cctgcacgag
      661 gcctatcgcc gcgccgggca gcggcttgcc atggtcgacg aggcgctctc ggccgagacc
      721 ggctcgatcg cgcgcctgcc gtgggaggaa gtgcgcgact ggtttcacct ggaaaacaac
      781 tatgtcgaca gcctggaccg cgcggccgaa gcgctggccg ggcggctcgg cgcgctgtcg
      841 ccggaaaccg gagcgatcga ggcctacctg aaggacgcgc tgtcggtctc gctgatctac
      901 accggcctgc agggcctgcg gtcctacgat gcggagatgg gccacctgac ggtcgatccc
      961 ggccagccgc ccgaaagccg ccggttccag cttgcccacc agctcgccgc gctggcgctg
     1021 aaggacgaga ttgccgcggt ggtggagaac gcccggctga acagcgccgc cagccgccag
     1081 ctgctgttcg tcggcctgtg caactatgcc gccggggcgc tgctgatgcc ctaccgccgg
     1141 ttccgggccg aggcgcgcag cgtgcgccac gatattgacc ggctggccca gatcttcggc
     1201 acgagtttcg agcagacctg ccaccggctt tccaccctgc agcgcgagga cgcgaagggc
     1261 gtgccgttct tcttctgccg ggtcgacatg gccggcaaca tcaccaaacg ccattccgcc
     1321 acccgcctgc aattcgcccg gttcggcggg gcctgtccgc tgtggatcgt gcacgaggcg
     1381 gtggcgatcc ccgaccggat cctggtccag ctggccgaaa ccccggacgg ggtgcgctat
     1441 gtctcgatgg ccaaggggct ggtgaagccg agcggatcct accagcgcgc cccgcgccgc
     1501 tatgccgtgg cgctgggctg cgaggcagag cacgcgaacg agttcgtcta tgccgacggg
     1561 ctggacctga aggccgcccc cgccgccacc cggatcggca tcagctgccg catctgcccc
     1621 cgcgccgact gcgaccagcg cgccttcccg cccagcgacc gcccgatcag cgtcgatccg
     1681 gacagccgcg ggatcgtgcc gtatagggtg ggttaaacct tcacgatccc caagcggatc
     1741 atgtcgcgcg cggtgtcgag cacgctttcc tccaccgggc gcgggaccca gccgaggacg
     1801 tcgcgggcat ggttggccgg gctttcgcgg cgcttgccca gttcaccgat cacctggctg
     1861 atcaccggat cgaaccgggc ggcgatccgc accagccagt tgggcagctt cagcttgggc
     1921 accttgcgcg cctgcgggcc cagcccggcg cgcaggatcg cggcgacctc gtccagcatc
     1981 aggaacgggc cggaacagat gaaccgctct cccgccatgt tcggcgcggt caggcagcgc
     2041 acatgcatat ccgccacgtc gcgcacatcg acgatcccga agcccaggtt gggcatcccc
     2101 ggcatcgagc cttcgagcag cttcttgatc gcctccagcg aggtcgagaa atcgcccgat
     2161 tgcagcggcc cgaggacgag cgagggattg accgtgcaga actccatgtc gccgccttcg
     2221 gccgccaccc aatcgcgcgc ggcgcgttcg gcaatcgtct tggaacggac atagggatag
     2281 gcatccgggc tggtcggatc ggtccagtcg gcctcggtga acaccgattt tccgccgccg
     2341 tggccatagg caatcgcggc catcgaactg gtcatcacga accgcttgat ccccgccgcc
     2401 ttggccgccc gcagcgcgcg cagggcgccg tcgcgcgcgg gcacgatcag ctcgtcctcg
     2461 tggagcggct tgtcggccgg aagcggcgag gcgacgtgcg ccacgtggct gcacccggcc
     2521 atagcctcgg cccagccggc atcgctcatc aggtcggcgg cgaagaattt cagcttcgcg
     2581 ttatcaaccc ccaggctttc gcgcacttcg gcttcgcggg ccaggttgcg gatcgtggtg
     2641 tggaccgtcc agccctcggc gatcagctgc cggatcagga acccggcgat gtagccgctg
     2701 ccgcccgaaa caaagaccgt gcctgccatt tgccgtctcc ccctagaacc ggatcatgat
     2761 cgagcgcgcc agcgcgggcg agatcgagtg gaccacctgc agcagcttga ccatgccgac
     2821 gttgatcgtc tcggtgccgc gctccatgcc ggccacgatc tggcgggcgc attcggcggg
     2881 cggcatcttg ttgctgccgc gcccggcggt catctgggtg tccaccagcg gcggcagcgc
     2941 ttccaccacg gtgatcccgg tgcccttcaa ctgcgcgcgc accgccaggg tgtaggacct
     3001 caggcccgcc ttgctggcgc aatagaccgg cgatccggca ttgggggcca gggccaggcc
     3061 cgaagtcacg ttgacgatca tcgcctcccc gcgcgccttc agcaccggca aaagccgcgc
     3121 gatcagctgg gtcggcgcgt ggaggttgag gaagtaggtc ttctccgccc cggccagatc
     3181 gacctctccg ctgcggaaat cataggatgt ccccaccccg gcgttgttga ccagcacgtc
     3241 gatcggacgg cccgccagcc cggccagcag ggcctctacc ccctccggtc cggagaggtc
     3301 cgccgcgatc acttcgaacc cgtcggcccg ggtcgcggcg atacgatcgg gattgcggcc
     3361 cgaggcgatg acggtggcgc ccttgtcgcg caactgccgg atcaggtgcg cgccgatccc
     3421 gtcggtcgca ccggtgacaa gcacggtctt gccggccagt tgcatcgcaa tctccccaat
     3481 cgtcctttcg gcagagccta gccgattggt tgcggattgc aacctagcgc atcaggacca
     3541 gttcctcggc catcgagggg tgcagcgcaa cggtcgcgtc gaactgggcc ttggtcagcc
     3601 cggccttcac cgcaatcgcc gcggcctgga ggatttccgg cgcatccggg cccagcatgt
     3661 gcacgccgag caccacgtcg gacgccggat cgaccaccag cttgtaatag ccccgctccg
     3721 ccacggccga gaacatgttc tgcatcggcc ggaaatcgga cttgaacacc ttcacatggc
     3781 cgtggaccag ccgcgcctgt tcttcggtca ggccgaccgc ggcgatcggc ggctgggtga
     3841 agaccgcgct ggggatgtga tcgtaggcgg tggtccgcgg cgtgccaccg aacacggtgt
     3901 cggcaaaggc atggccctcg cggattgcca ccggggtcag ctggacccgg tcggtcacat
     3961 cgccgaccgc gtagatgctg gggcagctgg tctggttgta ttcgtcaacc ggcacttcgc
     4021 cgttcgcgcc aagggcgatc ccggcgttct ccagccccag cccttcggtc ttgggcttgc
     4081 ggccggtggc gaccatgacc gcatcggccg cgatcggcgc gccctcgccg gcatggacca
     4141 gaaagctgcc gtcgtgcacc ttttcgacct tgcggatcgg gcggttgaat ccgaattcga
     4201 tgccccggct ctgcaggatc ggcaggagcc gttcggtcag atcgtggtca tagccgcgca
     4261 ggatcttgtc gctgcggttg acgacggtca cctggctgcc cagcgcgttg aacacgccgg
     4321 cgaactccag cgcgatatag cccgcgccct ggatcacgat ccgcttcggc agttcgggca
     4381 gatggaacac ctcgttggag gtgatgcagt gctccgcccc gtcg
//
LOCUS       DASUDV010000197         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_403919,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000197 DASUDV010000000
VERSION     DASUDV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403919"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFV30_04195"
     CDS             <1..314
                     /locus_tag="VFV30_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4847319.1"
                     /translation="AVLAEKGIDAEIIDLRTLVPLDIETIEASVKKTGRCLIVHEATR
                     TGGFGGELSALVQERCFYYLEAPIERVTGFDTPYPHSLEWAYFPGPVRIGEAVDRLLK
                     A"
     gene            319..1593
                     /locus_tag="VFV30_04200"
     CDS             319..1593
                     /locus_tag="VFV30_04200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX4847320.1"
                     /translation="MGKFTFNLPDIGEGIAEAEIVAWHVKVGDRIEEDGKIADVMTDK
                     ATVEMESPVSGTILSIAGEEGDMIAIGSPLVVIETEGDDEAAATDTVAAEPVSEPEPA
                     PAPAPIAAAPTPAPAAPAEAPASPAAQVAEPHAKVLASPAVRQRAADLGIDLSQVKPA
                     EDGRVRHADLDAFLAYNAAGGFCAAGAKGVDQTVKVMGLRRRIAENMAASKRSIPHFS
                     YVEECDVTALEAMRAQLNSARGQRPKLTMLPLLITAICRALPDFPMLNARYDDEAGVV
                     QRFGAVHLGMATQTDGGLMVPVIRDAQDRNLWQLASEIGRLAEAARKGTATSAELSGS
                     TLTITSLGPLGGVATTPVINRPEVAIIGPNRIVERPMFVPDGMGGERIEKRKLMNLSI
                     SCDHRVVDGWDAASFVQAVKRLIETPVLLLAD"
     gene            1761..2354
                     /locus_tag="VFV30_04205"
     CDS             1761..2354
                     /locus_tag="VFV30_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX4847321.1"
                     /translation="MPSDPRIDAYIEQAAPFARPILTHLRALVLSAVPEAVETIKWGM
                     PHFTYQGKNLVGLAAFKAHCAFVLHGQGRQGGAEDGMGAYGKIASLADLPAEAELRAR
                     IAEACRAIDNPAVKAKTKAKPAPKPEIAMPDDFAAELARLQGARARFDAMAPSHRREY
                     LEWIVTAKRPETRTKRIAEAAEWIAEGKKRNWKYESC"
     gene            complement(2351..2845)
                     /locus_tag="VFV30_04210"
     CDS             complement(2351..2845)
                     /locus_tag="VFV30_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847322.1"
                     /translation="MRTAPLALALAAGLLAPASVASPAPASLNWLHGAWTGQSEFLGR
                     PAAVQLDVGPALQGTATALVYRVDAAASEGRPALRFEGRGTYRVAPDGTVTGQWADSG
                     GNLHPLSGRTTEGELQVNWGDVRSEMGHSRYSLGPDGVLTVTDSVYSRGTVRVFARAS
                     YRRR"
     gene            complement(2845..3219)
                     /locus_tag="VFV30_04215"
     CDS             complement(2845..3219)
                     /locus_tag="VFV30_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4847323.1"
                     /translation="MAIAFVPEMTASMGVTNLDAAIGWYESVLGFSLLYRADEIGWCE
                     MSTHMPGVNVGLSQVVDVPQGGGATNVWSVSDIEAAKAELDRLGVKQDGPIQTIPDLV
                     RLLTFFDPDGNAMMLAESLMQG"
     gene            3269..3799
                     /locus_tag="VFV30_04220"
     CDS             3269..3799
                     /locus_tag="VFV30_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847324.1"
                     /translation="MLSTLPLDLISALVRQRWLLPVLARFHGQPGLRFAELLGRLGLP
                     RDSLVRVLELAQAQGWIARNPGHGHPLRPEYILTEEGQGAARAAAELAARMEAAGLAP
                     ADLTRWSLPALRAIALGSERFNDLIRGLSPASPRALSQTLRALVANDLVVREVEDGFP
                     PSSRYRLTARGQALAS"
     gene            complement(3796..4242)
                     /locus_tag="VFV30_04225"
     CDS             complement(3796..4242)
                     /locus_tag="VFV30_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847325.1"
                     /translation="MTIRRNLAKIACALALAVPVVAVAAPLVALDSAVYVERVVAGKG
                     RMLQPAGTLKRGDRVVYVVSWYRMGGQGGFTVTNPLPKRVYFQGSADGREEVSIDGGK
                     TWGKLDSLRVGSRFATPEDVTHVRWRVPASEAARGQGQITYSAIVR"
     gene            4435..4511
                     /locus_tag="VFV30_04230"
     tRNA            4435..4511
                     /locus_tag="VFV30_04230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4469..4471,aa:Asp,seq:gtc)
BASE COUNT          766 a         1540 c         1598 g          731 t
ORIGIN      
        1 aggcggtgct ggccgaaaaa ggcatcgatg ccgaaatcat cgacctgcgc actctggtgc
       61 cgctggatat agaaacgatc gaggcctcgg tgaagaagac cgggcgctgc ctgatcgtcc
      121 acgaggcgac ccgcaccggc gggttcggcg gcgagctttc ggcgctggtg caggaacgct
      181 gcttctatta cctggaggcg ccgattgagc gcgtcaccgg cttcgacacc ccctatccgc
      241 acagcctgga atgggcttat ttcccgggtc ccgtccgcat cggcgaggcg gtggaccggc
      301 tgctgaaggc ataatctgat gggcaagttc acgttcaacc tccccgacat cggcgaaggc
      361 atcgccgagg ccgagatcgt cgcctggcac gtcaaggtgg gggaccggat cgaggaagac
      421 ggcaagatcg ccgacgtcat gaccgacaag gcaacggtcg agatggaaag cccggtttcg
      481 ggcacgatcc tctcgatcgc cggcgaagag ggcgacatga tcgcgatcgg atcgccgctg
      541 gtggtgatcg agaccgaggg tgacgacgag gctgcggcaa ccgacactgt cgcagccgag
      601 cccgtttcgg aaccggaacc ggccccggct cccgctccca tcgctgcggc acccacgccc
      661 gctccggctg cgcctgccga agccccggcg tcgcctgcgg cgcaggtcgc ggaaccgcat
      721 gccaaggtcc tggccagccc cgccgttcgc cagcgtgccg ccgatctcgg gatcgacctg
      781 tcccaggtaa agccggccga ggacggccgc gtgcgtcacg ccgatctcga cgccttcctg
      841 gcttacaacg ccgcgggcgg attctgcgcg gcgggcgcca agggcgtgga ccagaccgtc
      901 aaggtcatgg gcctgcgccg ccggattgcc gaaaacatgg ccgcgtccaa gcgcagcatt
      961 ccgcatttct cctatgtcga ggaatgcgat gtcaccgcgc tggaggcgat gcgcgcgcag
     1021 ctcaattccg cgcgtggcca gcgtccgaag ctgaccatgc tgccgctgct aatcaccgcg
     1081 atctgccgcg cgctgcccga tttcccgatg ctcaacgccc gctatgacga tgaggccggg
     1141 gtggtgcagc ggttcggcgc ggttcacctg ggcatggcga cccagaccga cggcgggttg
     1201 atggtgccgg tgatccgcga cgcgcaggac cgcaacctgt ggcagctggc gagcgagatc
     1261 ggccgcctgg ccgaagccgc gcgcaagggc accgcgacca gcgccgaact gtccggttcg
     1321 accctgacga tcacttcgct cgggcccctt ggcggagtgg cgacgacgcc ggtgatcaac
     1381 cgcccggaag tagcaatcat cggcccgaac cggatcgtcg agcggccgat gttcgtgccc
     1441 gatggcatgg gcggcgaacg gatcgagaag cgcaagctga tgaacctgtc gatcagctgc
     1501 gaccaccgcg tggtcgacgg ctgggacgcg gcgagcttcg ttcaggcggt caagcggctg
     1561 atcgaaaccc cggtgctgct gctggcggat tgatttgatc ctccctgtga gcgaagctca
     1621 tggggagggg gaccgtcacg cgtggcggga tggtggtggg gccagggagc ctcccgcgaa
     1681 ggcccctccg tcagccttgc gggctgccac ctccccatcg gctgtgccga cagggaggga
     1741 ctggaagggg aggaacgcca atgccgagtg accccaggat cgatgcctac atcgaacagg
     1801 ccgcgccctt tgcccggccg atcctgacgc acctgcgcgc gctggtcctt tcggccgttc
     1861 cggaggcggt cgagacgatc aagtggggca tgccgcactt tacctaccag ggtaagaacc
     1921 tggtcggcct ggccgcgttc aaggcgcact gtgcttttgt cctgcacggc cagggccggc
     1981 agggtggggc ggaggacggc atgggcgcct atggcaagat tgccagcctg gccgaccttc
     2041 cggccgaggc agaactgcgc gcccggattg ccgaagcgtg ccgcgccatc gacaatcccg
     2101 ccgtgaaggc gaagacgaag gccaagcccg cgcccaagcc cgaaatcgcg atgcccgatg
     2161 acttcgcggc cgaactggcc cggctgcaag gcgcccgcgc acggttcgat gccatggccc
     2221 cttcgcaccg gcgcgagtac ctggaatgga tcgtcacggc caagcggccg gaaacccgga
     2281 ccaagcggat tgccgaggcg gccgaatgga tcgccgaggg caagaagcgc aactggaagt
     2341 acgaaagctg ctagcgccgg cggtagctgg cccgggcgaa gacccggacc gtgccgcggc
     2401 tatagacact gtcggtgacc gtcagcaccc cgtccgggcc gaggctgtag cgcgaatgac
     2461 ccatctcgct gcgcacatcg ccccagttga cctgcagttc cccttccgtc gtccggcccg
     2521 acagcgggtg caggttgccg ccactgtcgg cccactggcc ggtcacggtg ccatcgggcg
     2581 cgacccggta ggtcccgcgc ccttcgaagc ggagggcggg gcggccttcg ctggctgcgg
     2641 cgtcgacccg gtaaaccagc gcggtggcgg tgccttgcag cgccgggcca acgtccagtt
     2701 gcaccgctgc cggccggccc aggaattcgg actgccccgt ccacgccccg tgaagccagt
     2761 taagggatgc cggggcgggg ctcgccacac tggcgggagc cagcaggccc gccgcgagtg
     2821 cgagcgcgag aggggctgtg cgcatcagcc ctgcatcagc gattcggcaa gcatcatcgc
     2881 gttgccatcg ggatcgaaga aggtcagcag cctgaccaga tcggggatgg tctggatcgg
     2941 cccgtcctgc ttcacgccca agcggtcaag ttcggccttg gcggcttcga tgtccgaaac
     3001 gctccagaca ttggtggcgc cgccgccctg cggaacgtcg accacctggc tgaggccgac
     3061 attcaccccc ggcatgtggg tgctcatctc gcaccagccg atttcgtccg cgcggtagag
     3121 cagagagaag cccagcacgc tttcgtacca gccgatcgcg gcgtcgaggt tggtcacgcc
     3181 catcgaggca gtcatttccg gcacgaaggc aatcgccatt tctgttctcc cttgtcgaat
     3241 cggtctgaaa aatatatttg ctttaaaaat gctgtcaacg ctgcctctcg atctgatttc
     3301 cgcgctggtc cgccagcgct ggctgctgcc ggtcctggcg cggtttcacg gccagccggg
     3361 gctgcgcttt gcggaactgc tgggccggct gggcctgccg cgggacagcc tggtccgggt
     3421 gctggaactg gcgcaggccc agggctggat cgcgcgcaac cccggacacg ggcatccgct
     3481 gcggccggaa tacatcctga cggaggaagg gcagggggca gcccgcgcgg cggcagagct
     3541 ggcggcgcgg atggaggcgg cggggttggc tccggccgat ctgacccgct ggagcctgcc
     3601 ggcgctgcgc gcgatcgcgc tgggcagcga acgcttcaac gacctgatcc gggggctgtc
     3661 cccggccagt ccgcgcgcgc tgtcgcagac gctgcgcgcg ctggtggcga acgatctggt
     3721 cgtgcgcgaa gtggaagacg gatttccccc gagcagccgc tatcggctga ccgcgcgggg
     3781 gcaggcgctg gcgtcctagc gcacgatcgc gctgtaggtg atctggccct ggccgcgcgc
     3841 ggcttcgctt gccggaacgc gccagcgcac gtgggtcaca tcctcgggcg tggcgaatcg
     3901 gctgccgacg cgcagcgaat cgagcttgcc ccaggtcttg ccgccgtcga tcgagacttc
     3961 ctcgcgccca tcggcgctgc cctggaaata gacccgcttg ggcaggggat tggtgacggt
     4021 aaagccgccc tggccgccca tccggtacca gctgaccaca tagaccacgc ggtcaccgcg
     4081 cttcagcgtg cccgcgggct gcagcatgcg gcccttgccg gccacgaccc gctccacata
     4141 aacggcgctg tccagcgcca ccagcggcgc ggcaacggca acaacgggaa cggccagcgc
     4201 cagggcgcag gcgattttcg caaggttgcg gcggatggtc acggttgtag ggcccccgaa
     4261 caaatcgtgc ttcacggcga agcggggcga ttgttcgcaa atccgggcaa aggaatggtt
     4321 aacgccggct ttaggacgtt tttcggggga ccgaacggct tgccgaaaat gccgcgcgaa
     4381 ccctgttgac agcctcggcg cgctggccta gtggcgcggc tcccaagtgg ctgcgcgggt
     4441 gtagctcagt tggttagagt gtcggcctgt cacgccgaag gtcgcgggtt cgagccccgt
     4501 cactcgcgcc acttgggaat tttccttacc atgcgctgct gaatttcacg gccttgccgc
     4561 ctgccggcag gctgacgatc atgccgtcgt ggccgatctt cagcgtgccg gtgaagacct
     4621 gcggcgcctt gccca
//
LOCUS       DASUDV010000198         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_94650,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000198 DASUDV010000000
VERSION     DASUDV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94650"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFV30_04235"
     CDS             <1..1071
                     /locus_tag="VFV30_04235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX4847326.1"
                     /translation="RTRRFAGSKDRRAVRELVYGAIRACGDVPVSGRAAMLRLAELEP
                     AIAPLFDGQGHGPAPVEPGEPVARGGVAPAWLEAELAASGITGTDAAALLGRAPLDVR
                     VNTLKADRATLTLPEPGEPLAAPQGLRFPGGTPVEQWDAYTRGLIEVQDGGSQLACLA
                     AAAQPGETVIDLCAGAGGKTLALAAAMDGRGRLIAADTDRTRLSRLAPRAERAGAGGI
                     ETRLLDPNREAEALADLAGAADAVLVDAPCSGTGTWRRNPEARWRLSPREIARYGAVQ
                     ARLLDLAATLVRPGGRVVFVTCSLLDAEGADQAAAFLARHPGWRAPALSLPAGTPRGQ
                     GWRLSPGADGTDGFFIACFVSP"
     gene            1110..1622
                     /locus_tag="VFV30_04240"
     CDS             1110..1622
                     /locus_tag="VFV30_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4847327.1"
                     /translation="MRYSPAAFALALLVAVTASTGHSAPPPAPLDPRAAALVAEGRKA
                     LAEGRVDEAVDSFEAALAIQPGHVAIYLNLAEATRKQGMQGKALHYYRVALQIDPENQ
                     YAIAGEGETLVEKGAVEKARRNLARLEQLCGNKTCPPARQLAAAIERGPTPQVVTAEQ
                     VEPKPQVSAN"
     gene            complement(1723..2223)
                     /locus_tag="VFV30_04245"
     CDS             complement(1723..2223)
                     /locus_tag="VFV30_04245"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX4847328.1"
                     /translation="MSDPFAHLPYRPCVGVMLVNPAGQAFVGRRIDTRGQPDEGGVYW
                     QMPQGGVDDGEDLREAALRELWEETGVEERHVTLLAQTREEVLYDLPEELMGKLWKGR
                     YRGQRQHWFLARFAGEDADIHLERHDPPEFAEWKWVDPLLLPDLIVPFKKRVYRSVLD
                     EFRDLI"
     gene            complement(2255..3193)
                     /locus_tag="VFV30_04250"
     CDS             complement(2255..3193)
                     /locus_tag="VFV30_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847329.1"
                     /translation="MSDTTPLIREDVQGFLALVNSLPAPERLDPAEQRAGYLGLKAMT
                     EADPRPMAVIKDLTCPGPAGDIPVRFYDVRESREPSPVILFFHGGGFVIGDLDTHNSL
                     CTELSAELDMPVLAVHYRLAPEHPFPAAPDDCEAAARWLAASPAELGRTVTGLVTIGG
                     SAGGNLATVTARALIDQPAAAPMLLQVLLYPATDESQDGSMERFAEGHLLTKVAMDWF
                     YSLYLPKSGDPRAFPLHAKAKGMCPTIVATAGLDPLQDQGRRMAAKLVDAGVDTLFLH
                     MHGLTHDFSTTRKALPSAAAATAKVIAAMKLMLMKV"
     gene            3380..>3615
                     /locus_tag="VFV30_04255"
     CDS             3380..>3615
                     /locus_tag="VFV30_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX4847330.1"
                     /translation="MATQTAEAATTTKNSPEAVVVRFAGDSGDGMQLTGGQFTLSSAL
                     AGNDFATFPDFPAEIRAPQGTLFGVSAFQINFGST"
BASE COUNT          542 a         1225 c         1278 g          570 t
ORIGIN      
        1 cgcacccgcc gctttgccgg cagcaaggac cgccgcgcgg tgcgcgagct ggtctatggc
       61 gcgatccggg cctgcggcga tgtgccggtc agcggccgtg cagccatgct gcggctggct
      121 gagctggaac ccgccattgc cccgctgttt gacgggcagg gccacggacc cgcgccggtt
      181 gaacccggcg agccggttgc gcgcggcggg gtcgctccgg cctggctgga ggctgagctg
      241 gctgccagcg ggatcaccgg caccgatgcg gcggcgctgc ttggccgcgc gccgctggac
      301 gtccgggtca acacgctcaa ggccgatcgt gcgacgctga ccctgcccga gcccggcgag
      361 ccgctggccg cgccgcaagg gctgcgcttt ccgggcggga ccccggtcga gcaatgggac
      421 gcctacaccc gcggcctgat cgaggtgcag gacggcggct cgcagctggc ctgcctggcc
      481 gcggcggcgc agccgggcga gacggtgatc gacctgtgcg ccggggccgg gggcaagacc
      541 ctggcgctgg ccgcggcgat ggacgggcgg gggcgcctga tcgccgccga taccgaccgg
      601 acccggcttt cgcgcctggc cccgcgggcc gaacgtgccg gggcgggcgg aatcgaaacc
      661 cgcctgcttg atccgaaccg cgaagccgaa gcgctggccg acctggccgg ggcggccgat
      721 gccgtgctgg tcgatgcgcc gtgcagcggc accggcacct ggcgccgcaa tcccgaggcc
      781 cgctggcgcc tctctccgcg tgaaatcgcg cgctatggcg cggtccaggc gcggctgctg
      841 gacctggctg ccacgctcgt ccggccgggc ggacgggtgg tcttcgtcac ctgttcgctg
      901 cttgatgccg aaggcgcgga ccaggcggcc gcctttctgg cgcggcatcc cggctggcgc
      961 gcgccggccc tgtcgctgcc tgccggaacg ccccgcgggc agggctggcg cctgtccccc
     1021 ggagccgacg ggaccgatgg ctttttcatc gcctgtttcg tttcgccatg ataaacggcg
     1081 gtcaaatggc cacgctcaag gagcaaatga tgcgttattc tcccgccgct ttcgcgcttg
     1141 cgctgttggt tgcggttacc gcctctaccg gacacagcgc gccgccgccg gcgccgctgg
     1201 atccgcgcgc cgcggcgctg gtggccgaag ggcgcaaggc cctggccgaa ggccgggtgg
     1261 acgaagcggt cgacagtttc gaggcggccc tggcgattca gcccggccat gtcgcgatct
     1321 acctcaacct ggccgaggcg acccgcaagc agggcatgca gggcaaggcg ctgcactatt
     1381 accgcgtcgc gctgcagatc gatccggaga accagtacgc gatcgccggt gagggcgaga
     1441 ccctggtcga gaagggcgcg gtcgaaaagg cccgccgcaa cctggcccgg ctggaacagc
     1501 tgtgcggcaa caagacctgc ccgcccgcgc gccagctcgc cgccgcgatc gagcgcggcc
     1561 cgaccccgca agtggtgacc gccgaacagg ttgagccgaa gccgcaagtc tcggcgaact
     1621 gagccgtcgt cccgggcttg acccgggacc gttggcgttg tcaggcaagc tgtcggccaa
     1681 tcggccagcg gtcccgggtc aagcccggga cgacgcgtgg gttcagatca gatctctgaa
     1741 ctcatccagc accgagcggt agacccgctt cttgaacggc acgataaggt cgggcagcag
     1801 cagcgggtca acccatttcc attccgcgaa ttcgggcggg tcgtggcgtt ccaggtggat
     1861 gtcggcatct tcgccggcaa agcgcgccag gaaccagtgc tggcgctggc cgcggtagcg
     1921 ccccttccac agcttgccca tcagctcttc gggcaggtcg taaagcactt cctcgcgggt
     1981 ctgggcgagg agggtgacgt ggcgttcctc caccccggtc tcttcccaca gctcgcgcag
     2041 ggcggcttcg cgcaggtctt cgccatcgtc gaccccgcct tgcggcattt gccagtagac
     2101 gccgccctca tcgggctggc cgcgcgtgtc gatccggcgg ccgacaaagg cctggccggc
     2161 cggattgacc agcatcacgc caacacaggg acggtagggc aggtgggcaa agggatcgct
     2221 cattcgcgcg cccttagccc atgactgcga acacttaaac cttcatcagc atcagcttca
     2281 tcgccgcgat caccttggcc gtggcggcgg ccgcgctggg cagggccttg cgggtggtgc
     2341 tgaaatcgtg ggtcagcccg tgcatgtgga ggaacagggt atcgaccccg gcatcgacca
     2401 gcttggccgc catccgtcgg ccctggtcct gcagcggatc aagccccgcc gtggcgacga
     2461 tggtggggca catgcccttg gccttggcgt ggagcgggaa ggcgcgggga tcgccgctct
     2521 tgggcagata gagcgaatag aaccagtcca tcgccacctt ggtcaggagg tgcccttcgg
     2581 caaagcgctc catcgatccg tcctggcttt cgtccgtggc gggatagagc agcacctgca
     2641 gcagcattgg cgcggcggcc ggctggtcga tcagcgcgcg agcggtcacc gtggccaggt
     2701 ttccgccggc gctgccaccg atggtgacca gaccggtgac ggtccgcccc agttccgccg
     2761 ggcttgccgc cagccagcgc gccgccgcct cgcaatcgtc gggcgcggcg gggaaggggt
     2821 gttccggggc caggcggtag tggaccgcca gcaccggcat gtcgagctcc gccgacagtt
     2881 cggtgcagag cgaattgtgg gtatccagat ccccgatcac gaaaccaccg ccgtggaaga
     2941 acaggatcac cggcgaaggc tcgcggcttt cgcgcacgtc atagaaccgc accggaatgt
     3001 cccccgccgg gccggggcag gtcaggtcct tgatcaccgc catgggccgc gggtcggcct
     3061 cggtcatggc cttgaggccg agatagccgg cgcgctgctc ggccggatcg agccgctcgg
     3121 gcgcgggcag gctgtttacc agggcaagaa aaccttgcac gtcctcgcgg atcagggggg
     3181 tggtgtcgct catccgggaa tcctctcgct gtgctgttta atcgacaggt taagtcgcct
     3241 tttgccactt gactagccgc cttggcgcgc ctcttgtcga attggccgcg cgacagtttt
     3301 tcttgattgc gcgtgagtga gggtgaggac tagggcgctt cgcgaagagg gcgcaaggtc
     3361 ggcctgacaa ggaagaagca tggcaacgca aaccgccgaa gcagcaacta ccaccaagaa
     3421 cagtcccgaa gccgttgtcg tccgttttgc gggcgattcc ggggacggca tgcagctgac
     3481 gggtggccag ttcaccctgt cgagcgcgct ggcgggcaac gactttgcga ccttcccgga
     3541 tttcccggcg gaaatccgcg cgccgcaggg cacgctgttc ggcgtctcgg ccttccagat
     3601 caacttcggc tcgac
//
LOCUS       DASUDV010000199         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_757384,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000199 DASUDV010000000
VERSION     DASUDV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757384"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VFV30_04260"
     CDS             complement(<1..1529)
                     /locus_tag="VFV30_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX4847331.1"
                     /translation="MDAKQHLYLVDGSAYIFRAYHRLPPLTNPSGVPVGAVYGYTTML
                     WKLADDLNKADGPTHMAVILDASGKSFRNEIYAEYKANRPPPPEDLKPQFPLVRDATR
                     AFSLPCIEEQGLEADDLIASYARAACERGWDVTIVSSDKDLMQLVGPCGAGHIDMLDT
                     MKNVRIGIPEVEEKFGVVPDLVGDVLALMGDSVDNVPGIRGIGPKTATKLIQEHGSLE
                     AALAAAEGMKPSKLRDNLIEHADMARLSRVLVQLKEDCALPVALDDFRLGAIPPDPLA
                     AFLQEHGFNSLLKRLGDGRGSPERKVDLNPAKPVTAGAQPAQGGSRQPLADLPAIDRS
                     LYACVQDEAALAEWIQRAMAARLVAFDTETSALDAISADLVGISLATGPGQACYIPLR
                     HGGTDMFAEKPRQIDRARALVLLKPLLESDAVLKVGQNAKYDLNVLARHGIAVAPLDD
                     TMVLSFDLDAGRSVDGIGGGHGMDELAMRHLGHTCIAFKDVCGTGKTAISFAEVPLDR
                     AT"
     gene            1820..2104
                     /locus_tag="VFV30_04265"
     CDS             1820..2104
                     /locus_tag="VFV30_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX4847332.1"
                     /translation="MRKIVLAAVAALAVSACSQEAKDDAAAVATDAAAVATDAATDAA
                     AAGTEAAAKVTDAAKEGAAAVTSAAAEGADKVEGAAKEAEAVAEEAAKKM"
     gene            2241..3572
                     /locus_tag="VFV30_04270"
     CDS             2241..3572
                     /locus_tag="VFV30_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4847333.1"
                     /translation="MTTSLSEGLAKALQRIGLPAPQGLERLSGGANMESWRFAAGSET
                     CVLRRAPSLEMMAGRPMDHAGEAALIRAARAAGVMAPEVLVELLPEDGIGSGYVMRAI
                     SGSPDPVAFLAEADPQTAIRDIARELVATHRTDPAGLPVPVMDTAEALGELRRRFLEY
                     GGDRPILALALRWLEANIPPPVPPRLVHGDFRLGNLMFEHGRLTGVLDWELAHLGDWH
                     EDLAFGCMTVWRFSRPDRPGYGLTTVEELAAAYEEAGGERFDPERFRFWTIYRTFWWA
                     LGCLQMGGYWRDGHDRSVERVVVARRTAEQELDLLLLLEDLAPEEERARPLPPPQEPL
                     APSTGEPSGAEILTAISEWLASDIKPLVSGRGKFDLAVARNALGIVAREIEQRPLATD
                     AALAADLMSGKADLATPGLLARLRRMALAKLSADMPKYPALAAARAQWEGH"
     gene            3574..>4862
                     /locus_tag="VFV30_04275"
     CDS             3574..>4862
                     /locus_tag="VFV30_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847334.1"
                     /translation="MDFALPQDLVAYLAELDDFIAKEIKPLEQQDDNIRFFDHRREWA
                     RTNFEDQGLPRHEWEMLLREASLRADKAGHWRFSAPKKYGGKDGSNLWMAVIRDRFAA
                     MGLGLHNDLQNEHSIVGNFPFVAMFEHFGTPEQQEEFILGGFNRERRVAFGLTEPDHG
                     SDATHMETRAVRETRDGVPGWRIDGRKMWITGMHVATHCAAFCRTSGKDGDAKGITCL
                     LVPAGTPGMVVDEYMWTFNMPTDHPRMHFENCWVPESAVLGPVDGGLSIAQSFVHQNR
                     IRQAASSLGAATYCVEESVKYARHRKPFGQDLARNQGIQFPLVELATQCEMLRTLIYK
                     TAWEMDQLEHKEIEHKLSDKISMCNYYANRLVCEAADRAMQVHGGIGYSRHKPFEHIF
                     RHHRRYRITEGAEEIQMRKVAAYLFGYLGPRKALYAA"
BASE COUNT          797 a         1641 c         1623 g          801 t
ORIGIN      
        1 gtcgcgcggt cgagcggcac ctcggcaaag gagatcgccg tcttgccggt gccgcagacg
       61 tccttgaagg caatgcaggt gtggccgagg tggcgcatgg cgagctcgtc catgccatgc
      121 ccgccgccaa tcccgtccac gctgcgcccg gcgtcgaggt caaagctcag caccatggtg
      181 tcatccagcg gagccacggc gatcccgtgg cgggccagca cgttgagatc gtacttggcg
      241 ttctgcccca ctttcagcac cgcatcgctt tccagcagcg gcttcagcag gacgagcgcg
      301 cgcgcccggt cgatctgccg gggcttctcc gcaaacatgt cggtcccgcc gtggcgcagc
      361 gggatatagc aggcctggcc cggaccggtg gccaggctga tcccgaccag atcggccgaa
      421 atcgcgtcca gcgcgctggt ttcggtgtcg aaggccacca gccgggcggc catggcgcgc
      481 tggatccatt cggccagcgc ggcctcgtcc tgcacgcagg cgtaaaggct gcggtcaatc
      541 gcggggaggt ccgccagcgg ctggcggctg ccgccctgag cgggttgggc cccggcggtc
      601 accggcttgg ccgggttcag gtcgaccttc cgctccgggc tgccgcgccc gtcgcccagc
      661 cgcttgagca acgaattgaa gccgtgttcc tggaggaacg cggcgagcgg atcgggcgga
      721 atcgcgccca gccggaaatc gtcgagcgcg acgggcagcg cgcaatcttc cttgagctgg
      781 accagcacgc gggaaagccg ggccatgtcg gcatgctcga tcaggttgtc gcgcagcttg
      841 gacggcttca tcccctcggc cgcagccagg gctgcttcca ggctgccgtg ctcctggatc
      901 agcttggtcg cagtcttggg gccgatgccg cggatgcccg gcacgttgtc gaccgaatcc
      961 cccatcagcg ccaggacatc gccgaccagg tccggcacca ccccgaactt ttcctccact
     1021 tcgggaatgc cgatccggac gttcttcatc gtgtccaaca tgtcgatgtg gcccgcgccg
     1081 cacgggccga ccagctgcat caggtccttg tcggacgaca cgatcgtcac gtcccacccg
     1141 cgctcgcagg cggcgcgggc gtaggaggcg atcaggtcgt ccgcttccag cccctgttcc
     1201 tcgatgcagg gcaggctgaa ggcgcgggtg gcatcgcgga ccagcgggaa ctggggcttc
     1261 aggtcttccg gcgggggcgg acggttggcc ttgtattcgg cgtagatctc gttgcgaaag
     1321 ctcttgccgc tggcgtccag gatcaccgcc atgtgggtgg gcccatcggc cttgttcaga
     1381 tcgtcggcca gcttccacag catggtggta tagccataga ccgcaccgac cggcacgccc
     1441 gacgggttgg tcagcggcgg tagccggtga taggcgcgga agatataggc cgatccgtcg
     1501 accaggtaga ggtgctgctt ggcgtccatc gcgatgggtt agcaggcccg ggggcgaagg
     1561 gtaaggcccc gcttgcagcc gcgattcgtc ccgcgtcgcc ggctgtcaca ggcctgtcac
     1621 gggggcgcga tagagccgac aagcgccgga tttgcgccaa cttgaaggcc ccccgctaca
     1681 atctcttgca tagatggttt aaggacacta aatcacgccg cgaagtctac cacatcggta
     1741 ggctttccgt gcggtcgctc gcgccaaggt ggcaagatcg gtcgtgcgtg gtccactgtt
     1801 gttcaaggat tacaaagaca tgcgcaagat cgttctcgcc gctgttgccg ctctcgccgt
     1861 ttcggcctgc tcgcaggaag ccaaggatga cgccgctgcc gtcgccaccg acgctgctgc
     1921 cgtcgccacc gacgctgcca ccgacgctgc cgctgccggc accgaagctg ctgccaaggt
     1981 gaccgacgct gccaaggaag gcgctgctgc cgtgacttcg gctgccgccg aaggcgctga
     2041 caaggttgaa ggcgccgcca aggaagccga agccgttgcc gaggaagccg cgaagaagat
     2101 gtaatcttcg ccgcatggct aaccggatcg gggcgcgagg gcttggcctt cgcgcccctt
     2161 ttcgtttggc ggcttgactc gtttaatcgc gcgattaact ctgcgccccg accggtcgcc
     2221 aggaccggcg ggagagagca ttgaccacgt ccctgtccga aggcctggcc aaggccctgc
     2281 agcgcatcgg actgcccgcg ccgcaggggc ttgagcggct gtcgggcggg gccaacatgg
     2341 aaagctggcg ctttgctgcc ggcagcgaaa cctgcgtcct gcgccgcgcg cccagcctgg
     2401 aaatgatggc cggccggcca atggaccacg ccggagaggc cgcgctgatc cgcgccgccc
     2461 gcgccgccgg ggtgatggcc cccgaagtgc tggtcgagct tctgcccgaa gacggtatcg
     2521 gttcgggcta tgtcatgcgg gccatttccg gatcgcccga tccggttgcc ttcctggccg
     2581 aagccgatcc gcagaccgcg atccgcgaca ttgcccgcga actcgtcgcc acccaccgca
     2641 ccgatccggc cggcctgccc gtcccggtga tggatacggc cgaagccctg ggcgaactgc
     2701 gccggcgttt cctggaatac ggcggagacc ggccgattct ggccctggcg ctgcgctggc
     2761 tggaggccaa cattcccccg ccggtcccgc cgcggctggt ccacggcgat ttccgccttg
     2821 gcaacctgat gttcgaacac ggccgcctga ccggcgtact cgactgggaa ctggcccacc
     2881 tgggcgactg gcacgaggac ctggcctttg gctgcatgac cgtgtggcgc ttctcccggc
     2941 ccgaccggcc cggctatggc ctgaccacgg tggaggaact ggccgccgcc tatgaggagg
     3001 cgggcgggga gcggttcgat cccgaacggt tccgcttctg gacgatctac cgcacctttt
     3061 ggtgggcgct cggctgcctg cagatgggcg ggtactggcg tgacgggcat gaccgttcgg
     3121 tcgagcgcgt ggtcgtcgcc cgccgcaccg ccgaacagga gctggacctg ctgctgctgc
     3181 tggaagacct ggcgccggag gaagagcgcg cgcgcccgct gccgccgccg caggagccgc
     3241 tggcaccctc gaccggcgaa ccaagcggcg ccgaaatcct caccgcgatt tccgaatggc
     3301 tggccagcga tatcaagccg ctggtatcgg gccgcggcaa gttcgatctg gccgttgcgc
     3361 gcaacgcgct gggcattgtc gcccgcgaga tcgagcagcg cccgctggcc accgatgccg
     3421 cgctcgcggc cgatttgatg tcgggcaagg cggacctcgc cacgcccggg ctattggcca
     3481 gactgcgccg gatggcgctg gccaagcttt ccgccgacat gccgaagtac cccgcgctcg
     3541 cagccgcccg cgcccaatgg gaaggacact gaaatggact ttgcgctccc ccaggatctc
     3601 gtcgcctacc tcgccgaact cgacgatttc atcgccaagg agatcaagcc gctggagcag
     3661 caggatgaca acatccgctt cttcgatcac cgccgcgaat gggcccggac caatttcgag
     3721 gaccagggcc tgccccgtca cgaatgggaa atgctgctgc gcgaggcttc gctgcgggcc
     3781 gacaaggctg gccactggcg cttttccgcg ccgaagaagt acggcggcaa ggacggatcc
     3841 aacctgtgga tggcggtgat ccgcgaccgg ttcgccgcca tggggctggg cctccacaac
     3901 gatctgcaga acgagcattc gatcgtcggc aatttcccct tcgtggcgat gttcgaacat
     3961 ttcggcacgc ccgagcagca ggaggaattc atcctcggcg ggttcaaccg cgaacgccgc
     4021 gttgccttcg gcctgaccga gccggaccac ggatcggacg ccacccacat ggaaacccgc
     4081 gccgtgcgcg aaacccgtga cggggtgccc ggctggcgga tcgacgggcg caagatgtgg
     4141 atcaccggca tgcatgtcgc cacccactgc gcggccttct gccgcacctc gggcaaggat
     4201 ggcgatgcca agggcatcac ctgcctgctg gtgccggcgg gcacgccggg catggtggtc
     4261 gatgaataca tgtggacctt caacatgccg accgaccacc cgcggatgca tttcgagaat
     4321 tgctgggtgc ccgaaagcgc ggtgctgggt ccggtcgacg ggggcctgtc gattgcccag
     4381 tccttcgttc accagaaccg catccggcag gcggcgagct cgctgggcgc ggcgacctat
     4441 tgcgttgagg aatcggtcaa atatgcgcgc caccgcaagc ccttcggcca ggacctggcg
     4501 cgcaaccagg ggatccagtt cccgctggtc gagctggcca cccagtgcga gatgctgcgc
     4561 acgctgatct acaagaccgc ctgggagatg gaccagctgg agcacaagga aatcgagcac
     4621 aaactctcgg acaagatctc gatgtgcaac tactacgcca accggctggt ctgcgaggcg
     4681 gccgaccggg cgatgcaggt ccacggcggg attggttatt cccgccacaa gccgttcgag
     4741 cacatcttcc gccaccaccg ccgctaccgg atcaccgagg gggcggagga gatccagatg
     4801 cgcaaggtgg cggcctatct gttcggttac cttggcccgc gcaaggcgct ctacgccgcc
     4861 ca
//
LOCUS       DASUDV010000200         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_425933,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000200 DASUDV010000000
VERSION     DASUDV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425933"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..614
                     /locus_tag="VFV30_04280"
     CDS             138..614
                     /locus_tag="VFV30_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX4847335.1"
                     /translation="MASPAPSAEDLKAAQAVARGICRLFARNDIWCIPEMPLRSNRRA
                     DLMGIDAKGQLVIVEIKVSRADLLGDAKWTDYLEHCDRFYWGLAPELDRACMETEAFL
                     PERCGLIVADGYDAEILRPAATVALNAARRKAEVERLARTALRRHMASLDPTLSGW"
     gene            complement(615..788)
                     /locus_tag="VFV30_04285"
     CDS             complement(615..788)
                     /locus_tag="VFV30_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX4847336.1"
                     /translation="MTTDPLSPCTGVCRIDSATRLCAGCKRTIDEIMAWPRLAPETKR
                     AVLARLALRGRPA"
     gene            1089..>2072
                     /locus_tag="VFV30_04290"
     CDS             1089..>2072
                     /locus_tag="VFV30_04290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4847337.1"
                     /translation="MSYFTDLGLAEPLLRALGTKGYSEPTPIQRQAIPALLDGRDLLG
                     IAQTGTGKTAAFSLPSLHRLAADPQPRRNASCRMLVLSPTRELAAQIAENMRGYARYL
                     NLSVQCVFGGIPVGKQARALVPGCDILVATPGRLLDLLDQRALTLRNVEIFVLDEADQ
                     MMDLGFIVPLKRIANLLPKERQSLFFSATMPKAIADLGKQFIHNPVRVEVTPQATTAE
                     RVEQYAVFLNQSEKQALLSISLKKGIATGEIDRALVFTRTKHGADRVVRHLSAAGVQA
                     AAIHGNKSQAQRTAALAAFRKGSTPVLVATDIAARGIDVTGVSHVFNYELPN"
BASE COUNT          336 a          722 c          649 g          365 t
ORIGIN      
        1 cgcttgcgct gtggcaggaa tactatgccg cccgcgccgc gctgatggcc ttgaccccgc
       61 ccccggccgc gcccacctcg cgccgcttta cctgatcgac gcttgccagg ttcccgctct
      121 gttcctatga gcggtccatg gccagccccg ctccatccgc tgaagacctc aaggccgccc
      181 aggcggtggc ccggggcatc tgccggctgt tcgcgcgcaa cgacatctgg tgcatcccgg
      241 aaatgccgct gcgctcgaat cgccgcgccg acctgatggg gatcgacgcc aagggccagc
      301 tggtaatcgt cgagatcaag gtcagccgcg ccgatctgct cggcgatgcc aagtggaccg
      361 actatctcga gcattgcgac cgcttctact ggggtcttgc gcccgaactt gaccgcgcct
      421 gcatggaaac cgaagccttt ctgccggagc gctgcgggct gattgtggcc gatggctatg
      481 atgccgagat cctgcgcccg gcggccacgg ttgcgcttaa cgcagcgcgc cgcaaggccg
      541 aggtcgaacg cctggcccgc accgcgctgc gccggcacat ggccagcctc gatccgacgc
      601 tttcgggctg gtaatcaggc tggccggccg cgcagggcta gccgggcgag caccgcacgc
      661 ttggtttcag gggcaagccg cggccaggcc atgatctcgt cgatcgtccg cttgcatccg
      721 gcacacagcc gggtggccga atcgatccgg cacacaccgg tacaggggct gagcggatcg
      781 gtcgtcatgg tggcgggcat caagcaggtt tgccagagcc gggcaagagc cgtgaccgac
      841 gcgcgcaatt ttcttgcttt ttgttgcaat gcacactagg ggggctgcag caatcggcgc
      901 gcgggccctg ttcccgccgc agtcccggca gcgtgaggtc aggcgtttca acgccagact
      961 tgcccggact tacggtcgcc gcattatcgc tgaagcttcc tttttcacgc agggtcgcat
     1021 cgaacgctac ccatgcttcg tggtctggac tggttccggg ccgcgccgct tcattttgaa
     1081 agattatcat gtcctatttt accgaccttg gtctggccga gcccctgctg cgcgcgcttg
     1141 gcaccaaagg ctattccgaa cccaccccga tccagcgaca ggccatcccg gcgctgctgg
     1201 acgggcgcga cctgttgggc atcgcccaga ccggcaccgg caagacggcc gccttctcgc
     1261 tcccctcgct gcaccggctc gccgccgatc cgcagccgcg ccgcaatgca tcgtgccgga
     1321 tgctggtgct ttcgccgacc cgcgaactgg ccgcccagat cgccgaaaac atgcgcggct
     1381 atgcccgcta tctgaacctt tcggtgcaat gcgtgttcgg cgggattccg gtgggcaagc
     1441 aggcccgcgc actggtgccg ggctgcgata tcctggtggc gacgccgggc cgtctgctcg
     1501 acctgctcga ccagcgcgcg ctgacgctgc gcaatgtcga gatcttcgtg ctcgatgaag
     1561 ccgaccagat gatggacctg ggtttcatcg tcccgctcaa gcggatcgcg aacctgctgc
     1621 ccaaggaacg ccagagcctg ttcttctcgg caaccatgcc caaggcgatc gccgatcttg
     1681 gcaagcagtt catccacaac ccggtgcggg tcgaagtcac gccgcaggcc accaccgccg
     1741 agcgcgtcga gcagtacgcg gtgttcctca accagagcga aaagcaggcg ctgctttcga
     1801 tcagcctgaa gaagggcatt gccaccggcg aaatcgaccg cgcgctggtc tttacccgga
     1861 ccaagcacgg tgccgaccgc gtcgtccggc acttgtcggc cgctggcgtc caggctgccg
     1921 cgatccacgg caacaagagc caggcccagc gcaccgccgc gcttgcggcg ttccgcaagg
     1981 gcagcacccc ggtgctggtc gcgaccgaca tcgccgcgcg cgggatcgat gttacgggcg
     2041 tcagccacgt gttcaattat gagctgccga ac
//
LOCUS       DASUDV010000201         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1265350,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000201 DASUDV010000000
VERSION     DASUDV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265350"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1517
                     /gene="pstC"
                     /locus_tag="VFV30_04295"
     CDS             126..1517
                     /gene="pstC"
                     /locus_tag="VFV30_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX4847338.1"
                     /translation="MSPALPLLLALALGLVAWLVARSRAWAFRKEAQAKGGTLHSLPN
                     YHGWYVALWAAVPAVLFALAWSSISPGLVLAKVLADPAAASLPAFGINRDSILSEAQA
                     VASGAAVSTFQPQSAALVEPFRAALTQFGTIGIVVTLLLGFAGGAFAFLRIRPDFTAR
                     TRVERAVMAGLLAASLVAVLTTLGILASLVFETARFFQFVSPLEFLFGTHWGPDPMID
                     PSNVRPDTYGALPLFWGTVFIGAVIAMLVAIPLGLMSAIYLTQYASANVRKWMKPMLE
                     ILAGVPTVVYGYFAALTVAPLIRDFGLAIGISSASSESALAAGVVMGIMIIPFVSSMA
                     DDSITAVPQAMRDGSLAMGATTSETIKKVLVPAALPGIVAGIMLAVSRAIGETMIVVM
                     AAGATARLTANPFESMTTVTFQIVAMLTGEGSFDHPATLSAFALGMVLFLVTLLLNFI
                     ALRVVKRFREAYE"
     gene            1510..>2034
                     /locus_tag="VFV30_04300"
     CDS             1510..>2034
                     /locus_tag="VFV30_04300"
                     /inference="COORDINATES: protein motif:HMM:NF023240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3333 domain-containing protein"
                     /protein_id="HEX4847339.1"
                     /translation="MSNALASAHLKKRYAAERRFKAVGFAAVLFSGLVLAFLLITMTS
                     NAIGGFKRWELRFPVDLASGALTVDASQLQGPDAMRVLEGAGLADVVTFAASKQVGDA
                     AAAQVQDGAWREVATALIADPARLNGTIEVSLPVSDDLAAGARGDGGDALKTMAAKLK
                     DEGKLVSAFDWGFLS"
BASE COUNT          276 a          718 c          657 g          383 t
ORIGIN      
        1 tgctggccaa gcagggcatg gtcgtggctc cggacgatgt cctggccaag agcaccaagg
       61 cggcgaccga gtttaccctg ctcgacccct cgacgctgaa gtaagcttgg cctagaagcg
      121 caccgatgtc gccagcactg cctctcctgc tcgccctggc cctgggcctg gtcgcctggt
      181 tggtagcacg ctcccgcgcc tgggctttcc gcaaggaagc ccaggcgaag ggcggcacgc
      241 tgcattcgct tcccaattac cacggctggt acgtcgccct ctgggccgcc gtgccggccg
      301 tgctgtttgc cctggcctgg tcctcgatca gccccgggct ggtgctggcc aaggtcctgg
      361 ccgacccggc ggcggcatcg ctgccggcct ttggcatcaa ccgcgattcg atcctgtccg
      421 aagcccaggc cgtggcctcg ggcgcggcgg tcagcacgtt ccagccgcaa tcggccgccc
      481 tggtggaacc gttccgcgcc gcgctgacgc agttcggcac gatcggcatc gtcgtcaccc
      541 tgctgctcgg ctttgccggc ggggcctttg ccttcctgcg gatccggccc gatttcacgg
      601 cccgcacccg ggttgagcgg gcggtgatgg ccggcctgct ggccgcgtcg ttggtcgcgg
      661 tgctgaccac gctcggcatt ctggcctcgc tggtgttcga aaccgcgcgc ttcttccagt
      721 tcgtcagccc gctggaattc ctgttcggca cccattgggg tccggacccg atgatcgacc
      781 ccagcaatgt ccgccccgat acctatggcg cgctgccgct gttctggggc accgtgttca
      841 tcggcgcggt gatcgccatg ctggtcgcca tcccgctggg cctgatgagc gcgatctacc
      901 tcacccagta cgcctcggcc aatgtccgca agtggatgaa gccgatgctg gagatcctgg
      961 ccggcgtgcc gaccgtggtc tacggctatt tcgccgcgct gaccgtggcg ccgctgatcc
     1021 gcgatttcgg tctggcgatc ggcatttcca gcgcctccag cgaaagcgcg ctggccgccg
     1081 gcgtggtgat ggggatcatg atcatcccgt tcgtctcctc gatggccgac gattcgatca
     1141 ccgccgtgcc ccaggcgatg cgcgacggca gcctggcgat gggtgccacc acctccgaaa
     1201 cgatcaagaa ggtcctggtc cccgccgccc tgcccggcat agtcgctggg atcatgctgg
     1261 cggtcagccg cgcgatcggc gagaccatga tcgtggtcat ggccgccggc gcgaccgcgc
     1321 ggcttaccgc caaccccttc gaatcgatga ccacggtcac cttccagatc gtcgccatgc
     1381 tgacgggcga aggcagcttt gaccatcccg ccaccctctc tgcctttgcg ctggggatgg
     1441 tgctgttcct ggtgaccctc ctgctcaatt tcatcgcgct gcgcgtcgtc aagcgcttcc
     1501 gggaagctta tgagtaacgc cctcgcctct gcgcacctca agaagcgcta tgccgccgaa
     1561 cgccgtttca aggcggtcgg gttcgcggcc gtgctgttct ccggcctggt gctcgccttc
     1621 ctgctgatca ccatgaccag caacgcgatc ggcgggttca agcgctggga actgcgcttt
     1681 ccggtcgatc ttgcctcggg tgcgctgacg gtcgatgcca gccagttgca gggccccgac
     1741 gcgatgcgcg tgctcgaagg cgccgggctg gccgatgtgg tgaccttcgc cgcctccaag
     1801 caggtgggcg atgccgcggc cgcccaggtt caggacggcg cctggcgcga agtcgccacc
     1861 gcgctgatcg ccgatccggc ccgcctcaac ggcacgatcg aagtctcgct gccggtcagc
     1921 gacgatctgg ccgccggggc ccgcggcgat ggcggcgatg ccctgaaaac catggccgcc
     1981 aagctgaagg acgaaggcaa gctggtcagc gcgttcgact ggggcttcct gtcc
//
LOCUS       DASUDV010000202         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1221608,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000202 DASUDV010000000
VERSION     DASUDV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221608"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFV30_04305"
     CDS             complement(<1..325)
                     /locus_tag="VFV30_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847340.1"
                     /translation="MGKPDRIDRLAQALVLIAALIALGNGLKMLVDPFGWYDLVGTVK
                     ATGPANAHFIRDIGLAFLLSAALLGYGGSNLALRWGAALAGAGWLLAHGLLHIWEVLA
                     GICSAS"
     gene            392..895
                     /locus_tag="VFV30_04310"
     CDS             392..895
                     /locus_tag="VFV30_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4847341.1"
                     /translation="MRMNQGVEWAIHACALLAPLWPERGLSLAALAEFHGVPGPYMAK
                     QMQLLSKAGLVRTSRGRSGGYALARAPVEINLWQIARAIDGPEPAFRCTEIRQQGPCA
                     TPRAECRQSCPIAAAFAGAEAAWRTSLEGVTLADVIVRVGEQSSPEHLMTSMAWLGQQ
                     VVEIGGV"
     gene            complement(892..1800)
                     /locus_tag="VFV30_04315"
     CDS             complement(892..1800)
                     /locus_tag="VFV30_04315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX4847342.1"
                     /translation="MTQLIFSNEPAKGWIVPSLAAPFLCVFILIVSMLPGDLTLANLG
                     LTDERGEPQSPVGFFIMLVSFSALALAVAGWVRWVEKRSLASAGLCGERGLRKFLIGL
                     AIGIAMMGAIVAAIALSGGYRVGELFPAFASPAALGWIFLLLAGFVVQASAEEFVFRG
                     WLFSAVMRRWNMAAAFILSSAAFTLVHFSPRATLPEVVMTFTFAIFACAWVRRADSIW
                     GVMGWHVGWNWFGGSGFDVPITGLETHLPALVVKMEPVGPAWLTGGAEGPEGSVFTLV
                     LLTAGILILLLRTNPRQPDGRAEQPR"
     gene            complement(1832..2701)
                     /locus_tag="VFV30_04320"
     CDS             complement(1832..2701)
                     /locus_tag="VFV30_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4847343.1"
                     /translation="MARFAFLTFTPNIPPMHSATPSSSPSRPVDAFEPIERITNRLVG
                     AAFSPHRHDTYTVALTMAGVQAFNYRGASRHSLPGQVLILHPDELHDGHCRDEAGFSY
                     WAAYVPPTHVQSVLGGTELPFVANGVSTNRGLIAAAMTMVIDCAGVADPAAYEDALYD
                     LAQAMNHEAGKAATGRTANRTAVWQAREFLDTAVVAGARLDELEQVTGCDRWQLSRDF
                     RALLGTSPYRYLQHRRVDLAKRLLRDGATLADAAHGAGFADQSHFGRTFRKAVGLTPK
                     EWLHSDLFRTIVL"
BASE COUNT          560 a          968 c          890 g          416 t
ORIGIN      
        1 tgctggccga acagatcccg gccagcactt cccagatgtg cagcaggccg tgcgcaagca
       61 gccagcctgc ccccgccagc gccgcacccc agcgcagcgc caggttgctg ccgccatagc
      121 ccagcagcgc cgcgctcagc aggaaggcca gaccgatatc gcggatgaag tgcgcattgg
      181 ccggcccggt cgccttgacc gtcccgacca ggtcatacca gccgaacgga tcgaccagca
      241 tcttgagccc gttgcccagc gcaatcagcg ccgcgatcag caccagggct tgcgccaggc
      301 gatcgatccg gtcaggcttg cccatggtca tgcttccctt tgcgactcaa ttcggatatt
      361 tcatatccat atttaatgga gccggcaagt catgcggatg aaccaggggg tcgaatgggc
      421 gattcacgcc tgcgcgctgc tcgccccgct gtggccggaa cgcgggctga gcctggccgc
      481 actggccgag ttccatggcg tgcccggccc ctatatggcc aagcagatgc agctgctcag
      541 caaggccggg ctggtccgca ccagccgcgg ccgcagcggc ggctatgcgc tggcccgcgc
      601 cccggtggag atcaacctgt ggcagatcgc ccgcgcgatc gacgggccgg aacccgcctt
      661 tcgctgcacc gaaatccgcc agcaaggccc ctgcgccacc ccgcgcgccg aatgcagaca
      721 gtcctgcccg atcgccgccg cctttgccgg ggccgaagcg gcctggcgca cgtcactgga
      781 aggcgtgacc ctggccgacg tgattgttcg ggtgggcgaa caatcgagcc cggaacatct
      841 catgacatcg atggcctggc ttgggcagca ggtggtcgag atcggcggcg tttagcgcgg
      901 ctgttctgcc cttccgtctg gctggcgcgg atttgtgcgc aacagcagga tcaggatacc
      961 cgccgtaagc agcacaagcg taaacacgct cccctcaggc ccctctgcgc caccggtgag
     1021 ccaggctggg ccgacaggct ccattttcac caccagggca ggcagatggg tttcgaggcc
     1081 cgtaatcggc acatcaaagc cgctgccgcc gaaccagttc cagccaacgt gccagcccat
     1141 caccccccag atcgaatccg cgcgccgcac ccaagcgcag gcgaagatcg cgaaggtgaa
     1201 ggtcatcacc acctctggca acgttgcccg gggcgaaaag tgcaccaggg tgaacgcggc
     1261 ggaactcagt atgaaggcgg cagccatgtt ccagcgccgc atcacggccg aaaacagcca
     1321 gccgcgaaaa acgaactcct ccgcgctcgc ctggacgaca aagcccgcga gcaacaggaa
     1381 gatccagccg agcgcagctg ggctggcaaa ggccggaaac agttcgccga cacggtatcc
     1441 gccggacagg gcaatcgccg ccacgatcgc gcccatcatc gcgatgccaa tggcgagacc
     1501 gatcaggaac ttcctgagcc cgcgctcgcc acacagcccg gcactggcga ggctgcgctt
     1561 ttcaacccag cgcacccagc cggcgaccgc cagcgccagg gcgctgaacg aaaccagcat
     1621 gatgaaaaag cccacggggc tctgcggctc ccccctttcg tcagtaaggc cgaggtttgc
     1681 gagcgtcaga tcgcccggca gcatgctgac gatcagaatg aaaacgcaaa ggaagggggc
     1741 ggccaggctc ggcacaatcc agcccttggc cggctcgttg gagaagatca gctgggtcat
     1801 gccgcatcgg ctaaccgata ggcggcaagg cctatagaac gatcgtgcgg aacagatccg
     1861 aatgcagcca ctccttgggc gtcaggccaa ccgccttgcg gaaggtgcgg ccgaaatggc
     1921 tctgatcggc aaagcctgca ccgtgggcgg catcggccag cgtcgcgccg tcgcgcaaga
     1981 gccgcttggc cagatcgacc cggcggtgct gcagatagcg ataggggctg gtgccaagca
     2041 gggcccggaa atcgcgcgac agctgccagc gatcacagcc agtcacctgc tcaagttcat
     2101 caagccgcgc accggcaacg acggcagtgt caagaaattc gcgtgcctgc cacaccgccg
     2161 tccggttggc ggtccgcccg gttgcggcct tgccagcctc gtggttcatg gcctgcgcca
     2221 ggtcgtagag cgcgtcctca taggcggcgg gatccgccac acccgcgcag tcgatcacca
     2281 tagtcatcgc cgccgcgatc aggccgcgat tggtcgagac accattggcc acaaagggca
     2341 gctcggtgcc gcccagcaca gactgcacat gggttggcgg cacgtaggcc gcccaatagc
     2401 tgaagcccgc ttcatcgcgg caatgcccat cgtgcagttc gtcaggatgg aggatcagca
     2461 cctgccccgg caacgaatgc cgcgacgccc cgcgataatt gaacgcctgc accccggcca
     2521 tggtcagggc gacggtgtag gtatcgtgcc gatgcggcga aaacgccgcg ccaaccagcc
     2581 ggttagtgat ccgctcgatc ggctcgaagg catcgaccgg ccgcgatggg ctcgaggaag
     2641 gcgtggcgga gtgcatcggt ggtatattgg gtgtgaaggt caggaaggca aacctggcca
     2701 tccacacaat tccatcgccc ccactgtcgt caccccggac ttgatccggg gctgggcttt
     2761 cttcccgcgg tctggcgccg cgcctcaaga ccaagccaaa ccccggatca agtccggggt
     2821 gacgggtgca gatg
//
LOCUS       DASUDV010000203         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_934329,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000203 DASUDV010000000
VERSION     DASUDV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934329"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..150
                     /locus_tag="VFV30_04325"
     CDS             58..150
                     /locus_tag="VFV30_04325"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847344.1"
                     /translation="MLGRFADSPVVKDAGSASQVFAVAGLSYSF"
     gene            complement(138..1010)
                     /locus_tag="VFV30_04330"
     CDS             complement(138..1010)
                     /locus_tag="VFV30_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4847345.1"
                     /translation="MSAAFETLLVEQEGPILWVRLNQPEVLNAYTNLMGAELVRAIQR
                     ADGDDAVKVVVLTGTGRVFCAGADVSAGAGAFDTESGEGAKNFGSAGGSRANSNFVGA
                     MMDSVKPLLVAYNGSAAGVGLTLTLPCDIRISADSAKFGCVFTRRGLVPEAGSAWFLP
                     RLVGLSTALKWCMTGALVPAQEAHAAGLVSELVPAEGLEARARELALEIAANCSPVAL
                     ALTRRMLWTFSGQDGPEGALAVDAPLNIALGARGDVHEGVTAFLEKRSPAFPLSVSAD
                     LPDTPWYAPRNQKL"
     gene            complement(1007..2218)
                     /locus_tag="VFV30_04335"
     CDS             complement(1007..2218)
                     /locus_tag="VFV30_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4847346.1"
                     /translation="MTTRALTPIDLSDTALYTEDRWREPFARLRAEAPVSWREDSPYG
                     GYWSVVTHELCQAVELDWETYSSQTGNITIADGVAGQEFPNFIAMDPPRHTDQRRVIA
                     AAFTPSQMQRRERQVRERTKELFDALPLGETFDWVSEVSIPLTIGMLAIVLDFPWDER
                     MDLRKWSDMASNVSPEVMNEEYSQKFFAEMGLMLARFDALLEARRAQEPADDILSRMV
                     HSEAMGNLTALERIANIALLIVGGNDTTRNSMSGLIEALHRFPEARAALKADRALISN
                     ASQEIVRWQSPVTHMRRTLTRDAELGGQQLKAGEKIVMWYLSANRDEQVFADAERFDP
                     ARANARRHIGFGHGIHRCVGARLAEIQLDTVIGELLDRGLVAQPQGAPVRLASPFLHG
                     ITSLPVRLVRE"
     gene            complement(2215..>3005)
                     /locus_tag="VFV30_04340"
     CDS             complement(2215..>3005)
                     /locus_tag="VFV30_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4847347.1"
                     /translation="ELSQRPMAEKLFVSARRGVWIFPKYYRGQPLDKNPAPAWLPKGL
                     KQWLGARMVKKLVGRMSDYGLPEPEIGPFESHGTVSGEFLVRAGSGDITMKGGIERLD
                     GKEVVFSDGSREAIDVIVWATGYDISFPFFDEPSFKADADNRPPPLYKRILKPGVPDL
                     FYIGLAQPLPTLVNFAEQQSKLVAAYLAGQYRPPEPAEMERVIKADEDYYTGQYYAAR
                     RHTIQLDFDHYVRALKKELEKGAKRAAAAGGALPVPGRELEEAA"
BASE COUNT          459 a         1035 c         1023 g          488 t
ORIGIN      
        1 ctgggcggca atccgcgcaa gggctggggc ctgttcggcc tggtcaacgg caagcggatg
       61 ctgggccggt tcgccgacag cccggtggtg aaagacgcgg gcagcgccag ccaggtcttt
      121 gccgtggccg ggctgtccta cagcttctga ttccgcggcg cgtaccacgg ggtatccggc
      181 aagtcggcgc tgaccgagag cgggaaggcg gggctgcgtt tctccagaaa cgcggtcaca
      241 ccttcatgca cgtcgcctct ggcgcccagc gcgatgttga gcggggcgtc gaccgccagc
      301 gcgccttccg gcccgtcctg acccgagaag gtccacagca tccgccgggt gagcgccagc
      361 gcgacggggg agcagttggc ggcaatctcc agcgccagct cgcgcgcccg cgcctccagc
      421 ccctcggccg gaaccagttc gctgaccaga ccggcagcgt gcgcttcctg cgccgggacc
      481 agcgccccgg tcatgcacca tttgagcgcg gtcgacaggc cgacgaggcg cggcaggaac
      541 caggccgatc ccgcttccgg caccagcccc cggcgggtga agacgcagcc gaacttcgcg
      601 ctgtcggcgg aaatgcggat atcgcagggc agggtcaggg tcaggccgac acccgcggcc
      661 gagccgttgt aggcaaccag cagcggcttc acgctgtcca tcatcgcgcc gacgaagttg
      721 gaattggcgc ggctgccgcc cgcgctgcca aagttcttgg cgccttcgcc gctttccgtg
      781 tcgaaagccc cggccccggc ggagacatcg gccccggcgc agaacacccg gccggtcccg
      841 gtcagcacca ccaccttgac cgcgtcatcg ccatcggccc gctggatcgc ccggaccagc
      901 tccgcgccca tcaggttggt ataggcgttg agcacctcgg gctggttcag ccggacccac
      961 aggatcgggc cctcctgctc aaccagcagg gtttcgaacg cggcgctcat tcccgcacca
     1021 gccgcaccgg cagactggtg atgccgtgca ggaacggact ggcgagccgc accggcgcac
     1081 cctgcggctg cgccaccagg ccgcggtcca gcagttcgcc gatcacggta tcgagctgga
     1141 tctcggccag gcgcgcgccg acgcagcggt ggatgccgtg gccaaagccg atgtggcggc
     1201 gggcattggc gcgggccgga tcaaaccgct cggcatcggc aaagacctgc tcgtcgcggt
     1261 tggccgaaag gtaccacatc acgattttct cgccggcctt cagctgctgg ccgcccagct
     1321 ccgcatcgcg ggtcagggtg cggcgcatat gggtgaccgg gctctgccag cggacgattt
     1381 cctgcgaggc gttggagatc agcgcgcggt cggccttcag cgcggcgcgg gcttcgggga
     1441 agcggtgcag cgcctcgatc aggccgctca tcgaattgcg ggtggtgtcg ttcccgccga
     1501 cgatcagcag cgcgatgttg gcaatccgct ccagcgcggt gagattgccc atggcttcgg
     1561 aatggaccat ccggctgagg atgtcgtcgg ccggctcctg cgcccggcgc gcctccagca
     1621 gcgcatcgaa ccgcgccagc atcaggccca tttcggcgaa gaacttctgg ctgtactcct
     1681 cgttcatcac ctcgggagag acgttgctgg ccatgtcgga ccacttgcgc aggtccatcc
     1741 gctcgtccca cggaaagtcg agcacgatcg ccagcatgcc gatggtcagc gggatggaga
     1801 cttcggacac ccagtcaaag gtctcgccca gcggcaaggc atcgaacagc tccttcgtcc
     1861 gttcgcggac ctggcgttcg cggcgctgca tctggctggg ggtgaaggcg gcggcgatca
     1921 cccggcgctg gtcggtgtgg cgcggcgggt ccatcgcgat gaagttggga aactcctgcc
     1981 cggcgacgcc gtcggcaatg gtgatgttgc cggtctgcga ggaataggtt tcccagtcca
     2041 gctccaccgc ctggcacagc tcgtgagtca cgaccgacca gtagccgcca taggggctgt
     2101 cctcgcgcca gctgaccggc gcctcggcgc gcagccgggc gaagggctcg cgccagcggt
     2161 cttcggtgta gagcgcggta tcgctgagat cgatcggggt cagggcgcgg gtggtcatgc
     2221 ggcttcctcc agttcgcgcc ccggcaccgg caaggcacct ccggccgcgg cggcgcgctt
     2281 ggcgcccttc tccagctctt tcttcagcgc gcggacatag tgatcgaagt ccagctggat
     2341 ggtgtggcgg cgggcggcgt aatactgccc ggtgtagtag tcctcgtccg ccttgatcac
     2401 ccgctccatc tcggccggtt cgggcggccg gtactggccc gccagatagg ccgcgaccag
     2461 tttggactgc tgctcggcaa agttgaccag ggtcggcagc ggctgggcca ggccgatgta
     2521 gaacaggtcc ggcactccgg gcttcaggat ccgcttgtaa agcggcggcg ggcggttgtc
     2581 ggcatcggcc ttgaagctgg gctcgtcgaa gaaggggaag ctgatgtcat accccgtggc
     2641 ccagacgatc acgtcgatcg cctcgcggct gccgtcgctg aacaccacct ccttgccatc
     2701 aagccgctcg atcccgccct tcatcgtgat gtcgcccgat ccggcccgga ccaggaactc
     2761 gcccgagacg gtgccgtggc tttcgaacgg gccgatctcc ggctcgggca ggccatagtc
     2821 gctcatccgc ccgaccagct tcttcaccat ccgcgcgccc agccactgct tcagcccctt
     2881 gggcagccag gccggggcgg ggttcttgtc gagcggctgg ccgcggtagt acttggggaa
     2941 aatccagacg ccccgccggg ccgagacgaa cagcttttcg gccatcggcc gctgcgacag
     3001 ttcgc
//
LOCUS       DASUDV010000204         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1354157,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000204 DASUDV010000000
VERSION     DASUDV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354157"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1600)
                     /locus_tag="VFV30_04345"
     CDS             complement(173..1600)
                     /locus_tag="VFV30_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX4847348.1"
                     /translation="MGQEIATTGFCADDFARFSAQLGTETALLRERWAGHGLSRQGPI
                     AGFELEAWLIDRNCFPAPHNQAFLARLADPQVVPELSRFNIELNGDPQPLAGDGLARL
                     EQALGHSWERCVANAHADVDTAIAIGTLPTLRESDLSLAMMTPSNRYAALNAELARQR
                     GGKPVRIDIASAIAGGEGLVSVHDDVMLEAATTSFQLHLQVPADEIAATFNASLALSA
                     PLLAISANSPFLFGKALWHETRIALFEAAFEQAPINGSLRRVSFGSGWCGADPTAQFA
                     ENLELFPPLLPIVSAEPAQRFGCLRLHNGTIWRWNRLLVGFDDDGQPHLRIEQRVMPA
                     GPSTIDMFANAALYYGAVHMLARRPVKVDHALPFAAARDNFYAAARHGLDAELAWLDG
                     QRLPARKVLVSLIALAREGLERLGVDAALVDRYLDVVSGRLHSGRTGAAWQLAHHARH
                     GDLFRLTADYLENQRSGAPVHEWAL"
     gene            1716..1847
                     /locus_tag="VFV30_04350"
     CDS             1716..1847
                     /locus_tag="VFV30_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847349.1"
                     /translation="MARAIALVAQTSLPPIVVLRPLVVYGCALALILAGPALPALGL"
     gene            complement(1836..2582)
                     /locus_tag="VFV30_04355"
     CDS             complement(1836..2582)
                     /locus_tag="VFV30_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4847350.1"
                     /translation="MIAGLSPEQIAIALAAALAAAFVRGLAGFGMAILLVPVLGLAIP
                     PAEAVVTGNWLGIFIGLVGLRQILSASERSAFIICGFAMAATPLGVWLLAQTDPAMAR
                     LLIALVALGAFVMVLLPKRDEGHQPSGSETGATGIACGVLTGFAGMPGVPVVPFYLRR
                     HLAPVVARASMMTIFLATSVAGVGSAVVLRVATVRELVLGLVLFPAVLLGNWLGHRAF
                     GRISDTAWRSFVGLVLGASALAALWRLLQT"
     gene            complement(2584..>3069)
                     /locus_tag="VFV30_04360"
     CDS             complement(2584..>3069)
                     /locus_tag="VFV30_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4847351.1"
                     /translation="WQVGTMQSDRVLPERLDASYVGEDGERHRPVMLHRAIFGSYERF
                     IGILIEHYAGKLPVWLAPVQAVVATIVSDADGYAAEVTRQLAEAGIRVETDVRNEKIN
                     YKVRDHSLKKVPHLLVVGKREAEEGTVALRTLGAEHQKVMPLAEAIALLKAEATPPDL
                     R"
BASE COUNT          538 a         1062 c          995 g          474 t
ORIGIN      
        1 cttcaagctg ctgatggcgg tgtccttcgc cgggctggtc tatgtcctgg cctggctggc
       61 ctggcgcaag gtttcgggca atccggtgcc acgcaaggcc gaggccgatg ccgacaagct
      121 gccctttggc gtggccatcg caatcggcgc cgtgctcacg cagctgtact agctaaagcg
      181 cccattcgtg caccggcgcg ccgctgcgct ggttttcgag gtaatctgcg gtcagccgga
      241 acagatcgcc gtggcgggca tgatgggcaa gctgccaggc cgcgccggtc cgccccgaat
      301 ggagccgccc ggacaccaca tcaaggtagc gatcgaccag cgccgcatcg acccccagcc
      361 gctccagccc ttcgcgggcc agcgcaatca ggctgaccag aaccttgcgg gccggcagcc
      421 gctggccatc gagccaggca agttcggcat caaggccatg gcgggcggcg gcatagaaat
      481 tgtcgcgcgc cgcggcaaag ggcagggcat ggtcaacctt gaccggacga cgcgccagca
      541 tgtgcaccgc gccgtaatag agcgcggcat tggcgaacat gtcgatcgtg ctcggcccgg
      601 cgggcatcac ccgctgctca atccgaaggt gcggctggcc atcatcgtcg aagcccacca
      661 gtagtcgatt ccagcgccag atcgtgccgt tgtgcagcct gagacagccg aaccgctgcg
      721 ccggctcagc gctgacgatc ggcagcaggg gcgggaacag ttcaaggttc tcggcgaact
      781 gcgcggtcgg atcggcgccg caccagccgc tgccgaagct gacccggcgc aggctgccgt
      841 tgatcggggc ctgttcgaac gccgcctcga acagcgcgat ccgggtttcg tgccacagcg
      901 ccttgccgaa caggaacggc gaattggcac tgatcgccag caatggcgcc gacagcgcaa
      961 gcgaggcgtt gaaagtggcg gcgatctcgt ccgcgggaac ctgaagatgc agctggaacg
     1021 aggtggtggc ggcttcgagc atgacatcgt cgtgcacgct gaccagcccc tcgcccccgg
     1081 caatggcgct ggcgatgtcg atccgcaccg gcttgccacc gcgctggcgc gccagttcgg
     1141 cattgagcgc ggcataccgg ttcgacgggg tcatcatcgc cagcgacaga tcgctttcgc
     1201 gcagcgtggg cagcgtgccg atcgcgatcg cagtgtcgac atcggcgtgg gcattggcca
     1261 cgcagcgctc ccagctatgc cccagcgcct gttcaagccg ggccaaccca tcgcccgcca
     1321 gcggctgggg atcgccgttc agttcgatgt tgaagcgcga cagttcgggc accacctgcg
     1381 gatcggccag ccgggccagg aaggcttggt tgtgcggggc gggaaagcaa ttgcgatcga
     1441 tcagccaggc ttccagctcg aacccggcga tcggcccctg gcgggacaat ccgtgcccgg
     1501 cccatcgctc gcgcagcagc gcggtttcgg tgccgagctg cgcggaaaac cgcgcgaagt
     1561 catccgcgca aaagcccgtt gtggcgatct cctgccccat gccctgcccc ttcccggccg
     1621 gttgccctgg agccttcgtg ccagcgcaat gcgggccgcg acaacgatct cgatcaattt
     1681 acgcttcggt aagacaaaca tcggagaata gggtcatggc ccgcgctatt gcccttgttg
     1741 cccagaccag tctgccgccg atcgtggtgc tgcgcccgct ggtggtctat ggctgcgcgc
     1801 tggccctgat cctggccggc ccagcgctgc ccgctctagg tctgtaacag ccgccacagc
     1861 gcggcgagcg ccgaggcgcc cagcaccagt ccgacaaagc tgcgccaggc agtatcgctg
     1921 atccgcccaa aggccctgtg ccccagccag ttgcccagca gcacggcggg aaacagcacc
     1981 agtcccagca ccagttcacg cacggttgcc acgcgcaaga ccacggctga tcccaccccg
     2041 gccaccgagg tggcgaggaa gatcgtcatc atcgaggcac gggcgacaac cggcgcgagg
     2101 tgccggcgca aatagaacgg caccaccggc accccgggca tcccggcaaa gccggtcagc
     2161 accccgcagg cgatcccggt agcgccggtt tcgcttcccg atggctggtg gccctcgtct
     2221 cgcttgggca gcaggaccat cacgaaggca cccagcgcca ccagtgcgat cagcagccgg
     2281 gccatggcgg gatcggtctg tgcgagcagc cagacgccga gcggggttgc cgccatggca
     2341 aagccgcaga tgatgaaggc cgaacgctcg ctcgcgctca ggatctggcg caggcccacc
     2401 aggccgatga agatccccag ccagttgccg gtcaccaccg cttcggcagg cgggatcgcc
     2461 agccccagca ccgggaccag caggatcgcc atgccgaagc ccgccagtcc ccgcacgaag
     2521 gcagcggcca gcgccgcggc cagcgctatg gcgatctgct cgggcgaaag tccggcgatc
     2581 aagtcagcgc aagtccggcg gggttgcttc tgccttcaac agcgcgatcg cctcggccag
     2641 tggcatcacc ttctggtgtt cggcgcccag cgtgcgcagc gcgacggtgc cttcctcggc
     2701 ctcgcgcttg cccaccacca ggaggtgcgg gaccttcttc aaggaatgat cgcgcacctt
     2761 gtagttgatc ttttcgttgc gcacgtcggt ttcgaccctg attccagctt cggccagctg
     2821 tcgggtaacc tctgcggcat agccatcggc atccgaaacg atcgtcgcca ccaccgcctg
     2881 aaccggcgcc agccagaccg gcagcttgcc ggcatagtgt tcaatcagaa tgccgatgaa
     2941 gcgttcgtag gagccgaaga tcgcgcggtg cagcatgacc gggcggtggc gctcgccatc
     3001 ctcgccgaca tagcttgcat cgagccgttc gggcagaacc cggtcggact gcatggtccc
     3061 aacctgcca
//
LOCUS       DASUDV010000205         6389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1530854,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000205 DASUDV010000000
VERSION     DASUDV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530854"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3496)
                     /locus_tag="VFV30_04365"
     CDS             complement(<1..3496)
                     /locus_tag="VFV30_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX4847352.1"
                     /translation="MMGEWQFWIDRGGTFTDVVARAPDGRVLTAKLLSEDPARAEDAA
                     IAAIRQLSGAGNGPLPPAAVRMGTTVATNALLERKGEPTVLAITRGFGDALRIGTQER
                     PELFVRRIVLPEPPHAKVIEISERVMADGTVLEPLDEAAASAAFAEAVAAGFRSIAIV
                     LMHGYRYPAHEAQLAELARAAGFSQVSVSHEVAPLIKLVGRGDTTLVDAYLSPVLRRY
                     VDQFTAALGEGNRALFMQSSGGLADGARFRGKDAILSGPAGGIVGMARTAEASGAPKV
                     IGFDMGGTSTDVSHYAGQFERDSEVRVANVRIRTPMLRIHTVAAGGGSICRFDAGRLV
                     VGPESAGAVPGPACYRNGGPLTVTDCNVMLGKLRPDHFPHLFGPNGDQPIDAEVVAAR
                     FAEMAAAIRDETGQVFTPEALAEGFIAIAVANMAHAIKQISVARGHDVTGYALSCFGG
                     AGGQHACLVADELAIDTVLVHPLAGVLSAYGMGLASEAAVREQTLAQPLAGGLLAADH
                     LAGQLAEQVQAELGGTAAEVRTSLFLRREGSENTIELPPADPDELASAFAAAHRAQFG
                     YDGDGALVIDRVRVEATAAGESSGALDWPLPEAAGPALETIDCYLGGATHRVPVYDRA
                     ALPAGFAAPGPLIVIDPLATTVVEPGWSLTVDADGTLRLTRTAPRAATVASGTEADPV
                     RLEIFNGLFMAIAEEMGSALARTAMSVNIRERLDFSCAIFDAAGRLVANAPHMPVHLG
                     SMGESVQAVLREREGGKDGRGIRRGDAYVLNAPYAGGTHLPDITVVMPVFVGEGLEPD
                     FYVAARGHHADVGGIAPGSMPPNSRNIGEEGVLLENVLLVDEGRFCRDELAAILSSGP
                     YPARNIEANLADLAAQVAACARGATGLERACAEHGADVVRAYMGHVHDNAEEAVRRLI
                     GRLEDGSFRYELDNGAVVCVAVRVDRAARQVTIDFAGTSDQLPGNFNAPFSVVRAAVM
                     YVLRTMIDDPIPMNEGCLAPVTILVPEGCMLRPRYPAAVVAGNVETSQVVTDALFGAF
                     GAMAAAQGTMNNLTFGNDRYQYYETIAGGSGAGPDFDGTSVVQTHMTNSRLTDPEVLE
                     TRFPILLEAFAIRRGSGGAGRHHGGDGALRRLRFLEKMEAGMLANRRRVPPFGLAGGA
                     PGAPGATRVERTSGEVEVL"
     gene            complement(3531..4028)
                     /locus_tag="VFV30_04370"
     CDS             complement(3531..4028)
                     /locus_tag="VFV30_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4847353.1"
                     /translation="MRKTIALAALALLAACQTTPKKPTFSKQQVAVLVAEGFKPVGEN
                     YELGIADKVLFAVDQSDLPAEGAAVVDKLAKALSGVGISGALVEGHTDSTGSDEYNQA
                     LSERRANTVKDALARGGLSPNGVRAQGFGESDPIESNDTDEGRAQNRRVVVVVTPLDA
                     VPVGK"
     gene            complement(4028..5251)
                     /locus_tag="VFV30_04375"
     CDS             complement(4028..5251)
                     /locus_tag="VFV30_04375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4847354.1"
                     /translation="MSAVRLPTIREMLGRVHLRLILFAVLLAAASLMLSGGLVIRSYA
                     QRNLDLVARTVAYTVEPAVVFDDRQAIVDGIVSVSDGGMASQVEVFDPAGRLIAHWQN
                     PRVSLPQWLLQSGNELLWRRPAVAPLIHSGTRIGEVRITGNPEGLLRFALAGAIIALT
                     TLALTVIATRILARRLQRDVIGPLEHVAEVAHAVRTERSFDRRVPVAGIAEIDRFGQD
                     FNALLAELQGWHATMTRENAELAERVLLDPLTGLGNRAAFEAALPRAVTAAVTASQSF
                     AVLYCDLDQFKAINDTHGHVAGDAVLVAVARRMQAVLRQQDQAFRLGGDEFAVLLAPV
                     DADETACRVAARLGQAMVPPIDLPGGQAICTGLSVGIATYPDDGLAPEELLRRADRAM
                     YSEKRARNHNNNGGQ"
     gene            complement(5248..5823)
                     /locus_tag="VFV30_04380"
     CDS             complement(5248..5823)
                     /locus_tag="VFV30_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX4847355.1"
                     /translation="MRRVDKRVVTTALLPAALLALGEMPPVATGDPLLTPFAMAGAAE
                     GGNYDPAVARVVQSLVEYTRWPSPRNPLRLCVAGPALHAGRLDGLRLADGRLIERRTL
                     PPAAIAPGACDAVYIGQLSPIAQRQLTTTLRGRGVMTVAEADPACRSQAMFCLSFSAQ
                     AVSFRLNVDAVARSGLRVDPRVLRLSTGGQP"
     gene            5901..>6389
                     /locus_tag="VFV30_04385"
     CDS             5901..>6389
                     /locus_tag="VFV30_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX4847356.1"
                     /translation="MPAPIDRTALREAYRMEEEACIAERLRQAAPASAVHGEAKRIAA
                     RLVEGARAHKASGLDAFLQTYGLGTDEGIALMCLAEALLRVPDAATADALIHDKLGGI
                     DWSEHLGESGSTFVNAATFSLMLTGQVLDQRRAKADGMGAVLKRAVGRLGEPVIRQAT
                     LQA"
BASE COUNT         1087 a         2233 c         2111 g          958 t
ORIGIN      
        1 caagaacctc aacctcacct gaggttcgct caacccgcgt tgcccccggc gcgcccggtg
       61 cgccgcccgc cagcccgaac ggcggcacgc ggcggcggtt ggcgagcatg ccggcttcca
      121 ttttctcgag aaagcgcagt cgccgcagcg cgccgtcgcc gccgtggtgg cgccccgccc
      181 cgcccgaacc gcgccttatg gcgaacgctt caagcagaat cggaaagcgc gtttcgagga
      241 cttcgggatc ggtcaggcgg ctgttggtca tgtgggtctg gacgacgctg gtcccgtcga
      301 agtctggacc cgcgccagag ccaccggcga tcgtctcgta atactggtag cggtcattgc
      361 cgaaggtcag gttgttcatc gtcccctggg ccgcggccat cgcgccgaaa gccccgaaca
      421 gcgcatcggt gacgacctgg ctggtctcga cattgccggc cacgaccgcg gccggatagc
      481 gcggacgcaa catgcagcct tcggggacca ggatcgtgac tggcgcaagg cagccctcgt
      541 tcatcgggat cggatcgtcg atcatcgtgc gcaggacata catcacggcg gcgcggacca
      601 cgctgaaggg ggcgttgaaa ttgccgggaa gctgatcgct ggttccggca aagtcgatcg
      661 tcacctggcg cgctgcccga tcgacccgca ccgcgacgca gaccaccgcg ccgttatcga
      721 gctcgtagcg gaagctgcca tcttccagcc gcccgatcag gcggcgcacg gcttcttcgg
      781 cattgtcgtg gacatgaccc atgtaggcgc gaacgacgtc tgccccatgt tcggcgcagg
      841 cccgctccag cccggtggca ccgcgcgcgc aggcggcaac ctgcgccgcg aggtccgcca
      901 ggttggcttc gatgttgcgc gccggatagg ggccggagga gaggatcgcc gccaactcat
      961 cgcggcagaa gcggccctcg tcgaccagca gaacgttttc cagcaggacg ccctcctcgc
     1021 cgatattgcg gctgttcggg ggcatcgatc cgggcgcgat cccgccgaca tcggcgtggt
     1081 ggccgcgtgc ggcgacgtag aagtcaggct ccagcccttc cccgacgaac accggcatga
     1141 ccacggtgat gtccggcagg tgcgtaccgc cggcataggg tgcgttgagg acataggcat
     1201 cgccgcggcg gatgccccga ccgtctttgc cgccctcccg ctcgcgtagc acggcctgga
     1261 cgctttcccc catcgagccg aggtggaccg gcatgtgcgg cgcattggcc accagccgcc
     1321 cggccgcgtc aaagatcgcg caggagaaat cgagccgctc gcggatattg accgacatgg
     1381 cggtgcgcgc gagcgccgag cccatttcct cggcaatcgc catgaacagg ccgttgaaga
     1441 tttcgaggcg gacggggtca gcctcggtcc cgctggcaac ggtggcggcg cgcggcgcgg
     1501 ttcgggtcag gcgcagggtg ccatcggcat cgaccgtcag cgaccagccc ggctcgacca
     1561 cggtagtggc cagcggatcg atcacgatca gcggccccgg cgctgcaaac ccggcgggca
     1621 gcgcggcgcg gtcgtaaacc ggaacgcggt gcgttgcgcc gccaaggtag cagtcgatcg
     1681 tttccagcgc cgggccggca gcttctggca gcggccaatc gagcgcgccc gaagattcac
     1741 cggcagcagt ggcctcaacc cgcacccggt cgatcaccag cgcgccatcg ccatcatagc
     1801 caaactgcgc gcggtgcgcg gcagcgaagg cactagccag ctcgtccgga tcggcgggcg
     1861 gcaattcgat cgtgttctct gaaccctcgc ggcgcaggaa cagactggtg cggacttctg
     1921 cggcagtgcc acccaattcc gcctgaacct gttcggcaag ctggcccgcc aaatggtcag
     1981 ccgccaaaag gccacccgcc agcggctgcg ccagggtctg ctcacgcacc gccgcttcgc
     2041 tggcgagacc catgccatag gccgagagca ccccggccag cggatggacc agcaccgtat
     2101 cgatcgccag ctcatcagcc accaggcagg cgtgctgccc gcccgcgccg ccaaagcagg
     2161 acagggcata gccggtcacg tcatgcccgc gggcgaccga gatctgcttg atcgcatggg
     2221 ccatgttggc aacggcaatg gcgatgaagc cttcggccag ggcttcgggc gtgaaaacct
     2281 gaccggtctc gtcccggatt gccgcagcca tctcggcgaa cctggccgca acgacttcgg
     2341 catcgatcgg ctgatcgcca ttggggccga acaggtgcgg gaaatggtca ggccgcagct
     2401 tgccgagcat gacgttgcag tcggtcaccg tcagcgggcc gccattccga tagcaggccg
     2461 ggccgggcac ggccccggcg ctttccgggc cgaccaccag ccgcccggca tcgaaccggc
     2521 agatcgagcc gccacccgct gcgacggtgt ggatccgcag catcggcgtg cggatccgaa
     2581 cgttggcgac gcggacctcg ctgtcgcgtt cgaactgccc ggcatagtgg ctgacgtcgg
     2641 tcgaggtgcc acccatgtca aagccgatga ccttgggcgc accgcttgcc tcggcagtgc
     2701 gagccatgcc gacgatcccg ccggcgggtc cggaaaggat cgcgtccttg ccgcggaagc
     2761 gggcgccatc ggccagcccg ccgctcgact gcatgaacag cgcgcggttc ccctcaccca
     2821 gcgcagcggt gaactggtcg acgtagcggc gcaagaccgg cgagagatag gcatcgacca
     2881 gcgtcgtatc cccgcgcccg accagcttga tcagcggggc aacctcatgg ctgaccgaaa
     2941 cctggctgaa cccggcggcg cgggccagtt cggccagctg cgcttcgtgg gccggatagc
     3001 gatagccgtg catcagcacg atcgcgatcg agcggaaacc ggcggccacg gcctcggcaa
     3061 aggccgcgct ggccgctgct tcatcgagcg gttccagcac cgtaccatcg gccatgaccc
     3121 gttcggaaat ctcgatcacc ttggcgtgcg gcggttcggg caggacgatc cggcgcacga
     3181 acagctcggg ccgttcctgg gtgccgatcc gcagcgcatc gccaaagccg cgggtgatgg
     3241 ccagcacggt cggctcaccc ttgcgttcca gcagggcatt ggtggcaacg gtggtgccca
     3301 tccgcaccgc ggcgggtggc aagggcccgt tccccgctcc actcaactgc cggatcgcgg
     3361 caatcgccgc atcctcggcc cgcgccgggt cttcggacag cagcttggcg gtcagcaccc
     3421 ggccatcagg ggcgcgggcc accacatccg tgaaggtacc gccgcgatca atccagaact
     3481 gccactcacc catcatgccg ccgtcatagg gcggctgacg ggagaggcaa tcacttgccg
     3541 accggcacag cgtccaaagg cgtcacgact accacgaccc ggcggttctg ggcgcggcct
     3601 tcgtcggtat cgttgctctc gatcggatcg ctctcaccaa aaccctgggc gcgcacgcca
     3661 ttcggcgaga gcccgcccct ggccagcgcg tccttgaccg tattggcgcg gcgttcggac
     3721 agcgcctggt tgtattcgtc ggacccggtg gaatcggtat gcccttcaac cagcgcgccg
     3781 ctgatcccca ccccgctcag cgccttggcc agcttgtcga ccaccgccgc gccttcggcc
     3841 ggcaggtcgc tctggtcgac cgcaaacagg accttgtcgg caatgcccag ctcatagttc
     3901 tcacccaccg gcttgaaccc ttcggccacc agcaccgcca cctgctgctt cgaaaaggtc
     3961 ggctttttcg gggtggtctg gcaggccgcc agcagcgcca atgcggccag cgcgatcgtc
     4021 ttgcgcatta ttgtcctccg ttgttgttat gattgcgtgc gcgtttttcg ctgtacatcg
     4081 cgcggtcggc gcggcgcagc agttcctctg gtgccagtcc gtcatcggga taggtcgcga
     4141 tgcctacgct caggccagtg cagatcgcct ggccacccgg cagatcgatc ggcggcacca
     4201 tcgcttggcc cagccgcgcg gcgactcggc aggcggtctc gtccgcatcg accggggcga
     4261 gcagcaccgc aaattcgtcc ccgcctagcc ggaaggcctg atcctgctgg cgcagcaccg
     4321 cctgcatccg ccgcgccacc gccaccagca cggcatcgcc ggcgacgtgg ccatgggtat
     4381 cgttgatcgc cttgaactga tcgagatcgc agtaaagcac ggcgaaggat tggctggcgg
     4441 tcaccgcggc agtgaccgcc cggggcaagg cagcctcaaa cgctgcccga ttgccaaggc
     4501 ccgtcagcgg atcgagcagg acccgttcgg ccaactcggc gttctcgcgc gtcatcgtgg
     4561 cgtgccagcc ttgcaactcg gccagcaagg cgttgaagtc ctgcccgaag cggtcaattt
     4621 cggcgatccc ggccacgggc acacggcggt caaacgagcg ttcggtccgc acggcgtggg
     4681 ccacctcggc cacgtgctcc agcgggccga tcacatcgcg ctgcaaccgc cgggccagga
     4741 tccgggtggc aataacggtc agcgccagag tggtcagggc gatgatcgcg ccggccaggg
     4801 caaagcgcag caggccttcg ggattgccgg taatccgcac ctcaccaatc cgggtcccgc
     4861 tgtggatcag cggtgccacg gccgggcggc gccacagcaa ttcgttgcct gattgcagca
     4921 gccactgcgg cagactgacc cgtggattct gccagtgggc aatcagtcga ccggccggat
     4981 cgaacacctc tacctggctg gccatgccgc cgtcggaaac cgagacgatg ccatcgacaa
     5041 tcgcctgccg gtcatcgaag accactgccg gttcgaccgt ataggccacg gtccgcgcca
     5101 ccagatcgag attgcgctgg gcatagctgc ggatcaccag tccgccactg agcatcaggc
     5161 tggcggccgc gagcagcacg gcgaacagga tcagccgcag gtgaacccgg cccagcattt
     5221 cgcgaatggt cggaagccgc accgcgctca tggctggcct cccgtcgaca ggcgcagcac
     5281 gcgcggatcg acccgcaggc ccgagcgggc aaccgcatcg acattcagcc ggaacgagac
     5341 agcctgggcc gaaaagctca ggcagaacat cgcctggctg cggcaggccg gatcggcctc
     5401 ggccacggtc atcacgccgc ggccacgcag ggtggtggtc aactgccgct gcgcaatggg
     5461 cgagagttgg ccgatataga ccgcatcgca ggcgcccggc gcaatcgcgg ccggcggcag
     5521 ggtgcgccgc tcgatcaggc ggccatcggc gagccgcaag ccatcgagcc ggcccgcatg
     5581 gagcgccggc ccggcgacgc agagccgcag cggatttctg gggctgggcc agcgggtata
     5641 ttcgaccagc gattgcacaa cccgggcaac cgccgggtca tagttgccgc cctctgccgc
     5701 accggccatg gcgaacggcg tcaacagggg atcgcccgtt gccacaggcg gcatttcgcc
     5761 cagcgccagc agcgcggcgg ggaggagagc tgttgtaacc acccgtttat cgacccgcct
     5821 caagcctttg cgcagaacaa cggcaattgc aaggcaatgc gggaattggt ttccgtcgca
     5881 accagtgcta ggcacccgcc atgcctgcac caatcgaccg taccgccctg cgtgaagcct
     5941 atcgcatgga ggaggaagcc tgcatcgccg agcgcctgcg ccaggcggca ccggcctctg
     6001 ccgtgcacgg cgaggccaag cgcatcgcag cgcggctcgt tgaaggtgcg cgggcgcaca
     6061 aggctagcgg gctcgatgct ttcctccaga cctatggcct gggcacggac gagggcatcg
     6121 cgctgatgtg cctcgccgaa gcgctgctcc gcgtgcccga tgcggccacg gcggacgccc
     6181 tgatccatga caagctgggc gggatcgact ggtcggagca cctgggtgaa agcggctcga
     6241 ccttcgtcaa tgccgccacc ttctcgctga tgctgaccgg ccaggtgctc gaccagcgcc
     6301 gcgccaaggc ggacgggatg ggcgcggtgc tgaagcgcgc cgtgggccga ctgggcgaac
     6361 cagtgatccg ccaggcaacc ttgcaggcg
//
LOCUS       DASUDV010000206         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1442277,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000206 DASUDV010000000
VERSION     DASUDV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442277"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFV30_04390"
     CDS             complement(<1..846)
                     /locus_tag="VFV30_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4847357.1"
                     /translation="MNGPIPTTMRAVLLTGFGGYDRLDLRSDVAVPFPGPGEVLIRIG
                     AAGVNNTDINTRIGWYSKDVAASTDAGGDTGFSEAGDDGWSGAAFSFPRIQGADCCGH
                     IVACGDGVPASRLGERVLVDPLLHLADGSVQYFGSECDGAFAEYTRVPSRNAVAVNSA
                     LCDAELTSFPCAYSAAENILHRTAPTAGETVLVTGASGGVGSAAVQLAKRRGARVIAV
                     TGADKAAQVRDLGADRLVDRGADLRAELGRDAVDVVVDIVGGPQFPALLDCLRPGGRY
                     GSSGAI"
     gene            complement(865..1587)
                     /locus_tag="VFV30_04395"
     CDS             complement(865..1587)
                     /locus_tag="VFV30_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX4847358.1"
                     /translation="MTLPGEAQLREDLAAAFRLAAQFGWHESVGNHFSAAVSEDGRHF
                     LLNRKWQHFASIRPEDLLLLDAEDPEVMNGPDAPDASAWTIHGTIHRLCPSARVVLHC
                     HPVHATALAMLADPTLKPLDNNTARFFNRVAIDKNFGGIADEAEEGVRLARALGNHKT
                     MLMGNHGLLCSGTTVAAAFEDMYYFERAAQTQLLAYASGRELAVLDDAVAEKTAQGWE
                     PYAGMADAHFGYLKSTLEPLPR"
     gene            complement(1584..2702)
                     /locus_tag="VFV30_04400"
     CDS             complement(1584..2702)
                     /locus_tag="VFV30_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4847359.1"
                     /translation="MSNGETVRLGEKGLELSLSGRESYFNYHWLRDNCRTSFDPATRE
                     RVFDIFHLPAPPRPRAARVADGALEIEWDGENHVSHYDLSWLERSIAGPRHDPADLPR
                     RAWYADHYPAIHRVPFADLASDKAAVAAWLKAILVEGIAFVTEMPDSDEALTDLVRHV
                     GHLRPTFFGEYFDVRTHIEPSNLAYTAKALELHTDTPAEEMAPGIQFLHCRANSVEGG
                     DNLFLDGVAVAEDFRHEAPEDFRILSETWVPFYCEHDTYDMRSRQRVIELDDKGAVSG
                     VTISQHMADVFDLPQHELDAFYPAFCRFGRKLTEDKYLMRFNIRAGECIVFDNHRIVH
                     GRDAYSATSGDRYLRGCYADRGELRSSYRILAGEGRFK"
     gene            2915..>3491
                     /locus_tag="VFV30_04405"
     CDS             2915..>3491
                     /locus_tag="VFV30_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4847360.1"
                     /translation="MGTAVITGAAQGVGLVTARRFAAAGYHVVLTDIQPIEAAVAGIA
                     AEGGSASGLSGDIAAAPFVESLAALVADSHGGADVLVNNAGISLIEPAEDTSLEQWQR
                     VMAVNLQGPFLLSQAFGRQMLARGSGSIVNVASVAGMGGVIHRAAYNTSKHGLLGLTR
                     TLAAEWGGRGVRVNAVCPGWIKTEMDVKDMGS"
BASE COUNT          567 a         1139 c         1222 g          563 t
ORIGIN      
        1 aatcgcgccg gatgagccgt agcgcccgcc agggcgcagg caatcgagca gcgccggaaa
       61 ctgcggtccg ccgacgatgt ccaccaccac gtcaaccgca tcacggccca gttcggcgcg
      121 cagatcggcg ccgcggtcga ccaggcggtc tgcgcccaga tcgcgcactt gcgcggcctt
      181 gtcggcaccc gtgaccgcga tcacccgggc cccgcggcgc ttggccagtt gcaccgccgc
      241 gctgccgacc ccgcccgaag cgccggtcac caacacggtc tcgcccgcag tcggcgcggt
      301 gcggtgcagg atgttctccg ccgccgaata ggcgcaggga aagctggtca gctcggcatc
      361 gcagagcgcg ctgttcaccg ccaccgcgtt gcggctggga acgcgggtgt attcggcaaa
      421 ggctccgtcg cattcggacc cgaagtactg gaccgaaccg tccgccaggt gcagcagcgg
      481 atcgaccagc acccgctcgc ccagccggct ggcgggcacg ccgtctccgc aggccacgat
      541 gtgcccgcag caatcggcgc cctggatccg gggaaaggaa aaggcggcgc ccgaccagcc
      601 gtcgtcgccc gcctcgctga agccggtgtc cccgcccgcg tcggtcgagg cggcgacgtc
      661 cttggaatac cagccgatcc gggtgttgat atcggtgttg ttgaccccgg ctgcgccgat
      721 ccggatcagc acctcgcccg gaccggggaa gggaaccgcc acgtcgctgc gcaggtccag
      781 ccggtcatag ccgccgaacc cggtcagcag aacggcccgc atggtggttg ggatcggtcc
      841 gttcatcggc gtgtccttgt gcagtcagcg gggcaggggt tccagcgtcg atttcagata
      901 gccgaaatgg gcatcggcca tgccggcata gggctcccag ccctgggcgg tcttttccgc
      961 cacggcatcg tccagcaccg ccagctcgcg cccgctggca taggccagca gctgggtctg
     1021 tgcggcgcgt tcgaagtaat acatgtcctc aaacgccgcg gccacggtgg tgccagagca
     1081 gagcaggccg tggttgccca tcagcatggt cttgtggttg cccagcgccc gggccagccg
     1141 cacgccttcc tccgcctcgt ccgcgatccc gccaaagttc ttgtcgatcg ccacccggtt
     1201 gaagaaccgc gcggtgttgt tgtcgagcgg cttcagcgtg ggatcggcca gcatggccag
     1261 cgcggtcgcg tggaccggat ggcaatgcag cacgacccgc gccgaagggc agagccggtg
     1321 gatcgtgccg tggatcgtcc aggccgaggc atcgggggca tcggggccgt tcatcacttc
     1381 ggggtcttcg gcatccagca gcagcaggtc ttcgggccgg atcgaggcga aatgctgcca
     1441 tttccggttc agcaggaaat ggcgcccgtc ctcggacacg gcggcgctga agtggttgcc
     1501 gaccgattca tgccagccaa attgcgccgc cagccggaac gcggcggcca gatcctcgcg
     1561 cagctgggct tcacccggca gggtcacttg aagcgtcctt ccccggccag gatccggtag
     1621 gaactgcgca gctcaccgcg gtcggcatag cagccgcgca gatagcggtc gccgctggtg
     1681 gccgaatagg catcgcggcc gtgcacgatc cggtggttgt cgaacacgat gcattccccg
     1741 gcgcggatgt tgaagcgcat caggtacttg tcttcggtca gcttgcggcc gaaccggcag
     1801 aacgcggggt agaaggcgtc gagttcgtgc tgcggcaggt cgaacacgtc ggccatgtgc
     1861 tggctgatcg tcacgccgga aacggcgccc ttgtcgtcca gctcgatcac ccgctggcgc
     1921 gagcgcatgt cataggtgtc gtgctcgcaa tagaacggca cccaggtttc gctgaggatg
     1981 cggaagtctt cgggggcctc gtgccggaaa tcctcggcca cggcgacgcc atcgaggaac
     2041 aggttgtccc cgccttcgac cgaattggcc cggcagtgga ggaactggat ccccggcgcc
     2101 atttcctcgg ccggggtgtc ggtgtgcagt tccagcgcct tggcggtata ggccaggttg
     2161 gacggctcga tatgggtacg aacgtcaaag tattcgccga agaaggtcgg gcgcagatgg
     2221 ccgacatggc gcaccagatc ggtcagcgcc tcgtcgctgt cgggcatctc ggtcacgaag
     2281 gcgatgcctt cgaccaggat ggccttcagc caggccgcca ccgccgcctt gtcgctggcg
     2341 agatcggcaa agggaacgcg gtggatcgcc ggatagtgat cggcatacca ggcccggcgc
     2401 ggcagatcgg cgggatcgtg acgcggcccg gcgatgctgc gttccagcca cgaaaggtca
     2461 tagtgcgaaa cgtggttttc gccgtcccat tcgatttcca gcgcgccatc ggcaacccgc
     2521 gcggcgcggg gccggggcgg ggcgggcagg tggaaaatgt cgaacacccg ctcgcgcgtg
     2581 gccggatcga acgaggtccg gcaattgtcg cgcagccagt ggtagttgaa atagctttcg
     2641 cggccggaca gcgacagctc cagccccttt tcgccaagcc taaccgtttc cccgttggac
     2701 acgctgtttc ccactcccga ttggtaccga ccttggcggc gtttcgtgca cccggcgcgc
     2761 cgcgctggca atgccgggtt ttcgcgccgc cgggcatatt accaacagtg atgatcgaca
     2821 taacagcgtt gtcacggggt cgaaggatcg cgggcatgct gctatcgcgg ggacgagggc
     2881 cgggacaagg ccggtcagga tgcggcaggg gacgatgggt acggcagtca ttacgggtgc
     2941 ggcgcaaggg gttggcctgg ttacggcacg gcggtttgcc gccgccggct atcacgttgt
     3001 cctgaccgac attcagccga tcgaggcagc cgtggccggc atcgcggccg agggcggcag
     3061 cgccagcggc ctgtccggcg atattgccgc ggccccgttt gtcgaaagcc tggccgcgct
     3121 ggtggccgac tcgcacggcg gtgccgacgt gctggtcaac aacgccggga tcagcctgat
     3181 cgagccggcc gaggacacca gcctggagca gtggcagcgg gtgatggcgg tcaatctgca
     3241 ggggccgttc ctgctctcgc aggcgttcgg ccggcagatg ctggcgcgcg gaagcggcag
     3301 catcgtcaac gtcgcctcgg tcgcagggat gggcggggtg atccaccgcg cggcctacaa
     3361 cacgtccaag catggcctgc tgggcctgac ccggaccctg gccgccgaat ggggcgggcg
     3421 cggggtgcgg gtcaacgcgg tctgtccggg ctggatcaag accgaaatgg acgtgaagga
     3481 catgggctcg g
//
LOCUS       DASUDV010000207         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1707282,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000207 DASUDV010000000
VERSION     DASUDV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707282"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFV30_04410"
     CDS             <1..313
                     /locus_tag="VFV30_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX4847361.1"
                     /translation="ERHRYGVVREFCGHGLGRLFHDAPEVIHAARAGTGPELKPGMFF
                     TIEPMINLGKPGVKLLEDGWTAVTRDRSLSAQFEHSIGITETGCEIFTKSPKGMDRPP
                     Y"
     gene            complement(310..969)
                     /locus_tag="VFV30_04415"
     CDS             complement(310..969)
                     /locus_tag="VFV30_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX4847362.1"
                     /translation="MSGQLFTLLRRDLALLVLGGRRGGVTLPILFFLAVAMLYPFAVG
                     PDPRVLARTGGGVIWVAALLAAILPLDRLIEPDLELGMFDQWSLRGISEEAIIAVRVL
                     AHWLSFAPALMLAALPAAALVGIGGETLWLVELGLLAGTPGLAALGVMVAALTAGLRG
                     GAALGGLLVIPLAVPLLIFGAGSLATGGEAGLALTGAVSLALLAVAPFAGGAAIRAGR
                     G"
     gene            complement(966..1544)
                     /gene="ccmA"
                     /locus_tag="VFV30_04420"
     CDS             complement(966..1544)
                     /gene="ccmA"
                     /locus_tag="VFV30_04420"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX4847363.1"
                     /translation="MQECRLVATEIACRKGDRLLFAGLSLDLKGGMALQLTGANGIGK
                     SSLIRILAGLATPFAGTVERDGAIGLVDERPALDPHLPLGRALALWQRIDGPADNEAA
                     RLGIAGLHDVPVRFLSTGQKKRAALARLLGQKAPIWLLDEPLNGLDARGVTLVEELAG
                     EHCAEGGICVIASHQPFTLPGMAQIALKDFAA"
     gene            1594..1884
                     /locus_tag="VFV30_04425"
     CDS             1594..1884
                     /locus_tag="VFV30_04425"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX4847364.1"
                     /translation="MSLSRLSDAEVASLLAENPGWTLREDGKAIIRNFKFKDFNQAFG
                     FMTRVALYADKADHHPEWFNVYNRVEMTLTTHDADGLSRRDVAMVKAIEGML"
     gene            complement(1896..2702)
                     /locus_tag="VFV30_04430"
     CDS             complement(1896..2702)
                     /locus_tag="VFV30_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4847365.1"
                     /translation="MASRKCIFITGGASGIGRAVAVKFGAQGWFVGLADVNEAGMRET
                     AAMLPAGASSVHLLDVRDRTAWDAALAACAKASGGRIDVVFNNAGIPLGGPLIENNVT
                     EIERCLDINLKGVLFGAQAAHPYLKASAPGSCLLNTASAAGIYGTPGASVYSATKFGV
                     RAITECLDAEWFEDGIKVCDLMPGFIDTPLIDMNPNAQSNEDIRTRVTEAGIEITPAS
                     EVAETAWKAVHGNDLHNRVGKTAHRLAFAARWLPGRLRKQTRQSLKPLGG"
     gene            complement(2779..>3075)
                     /locus_tag="VFV30_04435"
     CDS             complement(2779..>3075)
                     /locus_tag="VFV30_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847366.1"
                     /translation="KRLMLLAVFAILDTGWSRWLGRDLTSVLGQGLARETLGRFPLTW
                     ALMTAMALYDIKTRGRLHPAFLPAAGLILATQIGSIGLFFAPGWPETARALLGI"
BASE COUNT          548 a         1072 c          987 g          468 t
ORIGIN      
        1 cgaacgccac cgttacggcg tggtgcgcga attctgcggg catggcttgg gccggctgtt
       61 ccacgatgcc cccgaagtga tccacgccgc gcgcgctggc accgggccgg aactgaagcc
      121 cggcatgttc ttcacgatcg agccgatgat caacctgggc aagcctgggg tgaaactgct
      181 cgaagacggc tggacggcgg tgacgcgcga ccgttcgctt tctgcccagt tcgaacattc
      241 gatcggaatc accgagaccg gctgcgaaat cttcaccaag agccccaagg ggatggaccg
      301 gccgccttac tagccccggc ccgcccggat cgccgcgccg ccggcaaagg gagcgacagc
      361 cagcagtgcg aggctgaccg cgccggtcag tgccagcccg gcctcaccgc cggtggcaag
      421 gctgcccgcg ccaaagatca gcagcggcac ggcgagcggg atcaccagga ggccgccgag
      481 cgccgccccg ccgcgcaggc cagcggtcag cgcggcgacc ataaccccca gcgcggcaag
      541 ccccggggtt ccagccagca gccccagttc gaccagccac agcgtttcgc cgccgatgcc
      601 caccagcgcc gcggcgggca gcgcggcgag catcagtgcg ggcgcgaagc tcagccagtg
      661 ggcgagcacc ctgaccgcga tgatcgcttc ctcgctgatc ccgcgcaggc tccattgatc
      721 gaacatgcca agttccagat cgggttcgat cagccggtcg agcggaagga tcgcggcaag
      781 cagcgccgcc acccagatca ccccgccccc ggtgcgcgcc agcacccttg gatcgggccc
      841 gaccgcgaag ggatagagca tcgccaccgc gaggaagaac aggatcggca gcgttacccc
      901 gccgcgcctg ccgcccagca ccagcagcgc cagatcgcgc cgcaggagag tgaagagctg
      961 gccgctcatg ccgcgaaatc cttcaaggcg atctgcgcca tgcccggcag ggtaaagggc
     1021 tggtgcgatg cgatcacgca gatcccgccc tcggcgcaat gttcgccggc cagttcctcg
     1081 accagcgtca cgcccctggc gtcgagcccg ttgagcggct cgtcgagcag ccagatcggc
     1141 gctttctggc cgagcagccg ggccagcgcc gcgcgcttct tctggccggt ggacaggaac
     1201 cgcaccggca catcgtgcaa tccggcgatg cccagccgcg cggcttcgtt gtcggccggc
     1261 ccgtcgatcc gctgccacag cgccagcgcc ctgccgagcg gaagatgcgg atcgagcgca
     1321 ggccgttcat cgaccaggcc gatcgcgcca tcgcgctcga ccgttccggc aaaaggcgtc
     1381 gcaagcccgg ccaggatccg gatcaggctg ctcttgccga tcccgttggc gccggtgagc
     1441 tgcagcgcca tgccgccctt cagatcgagc gaaagccccg cgaacagcaa gcgatcgccc
     1501 ttccggcagg caatttcggt tgcgacaagg cggcactctt gcatggggca gcgcgatagg
     1561 ggaaagctgc gcgcctgcca aggagacccg cgaatgtcgc tttcccgcct gtccgatgcc
     1621 gaagttgcca gcctgcttgc cgaaaacccc ggctggaccc tgcgtgaaga cggcaaggcg
     1681 ataatccgca atttcaagtt caaggatttc aaccaggcct tcggcttcat gacccgcgtc
     1741 gcgctctatg ccgacaaggc cgatcaccat cccgaatggt tcaacgtcta caaccgggtc
     1801 gagatgacgc tgaccaccca cgatgccgac ggcctcagcc ggcgcgacgt ggcgatggtc
     1861 aaggcgatcg aggggatgct ctagattcag cagattcagc cgcccagcgg cttcaggctt
     1921 tggcgggtct gcttgcgcag gcggcccggc aaccagcgcg cggcgaaggc gaggcggtgc
     1981 gcggtcttgc ccacgcggtt gtgcagatcg ttgccgtgca ccgctttcca cgcggtttcc
     2041 gccacttcgc tggccggggt gatttcgatg cccgcttcgg tcacccgggt gcggatatcc
     2101 tcgttcgact gggcgttggg gttcatgtcg atcagcgggg tatcgatgaa gccgggcatc
     2161 agatcgcaaa ccttgatccc gtcctcgaac cattcggcgt cgaggcattc ggtgatcgcc
     2221 cgaaccccga acttggttgc cgaatagacc gaagcgcccg gcgtgccata gattcccgcc
     2281 gcgctcgcgg tgttgagcag gcacgatccg ggggcgctgg ccttgaggta gggatgcgcc
     2341 gcctgcgcgc cgaacagcac gcccttgaga ttgatatcga ggcagcgctc gatctcggtc
     2401 acgttgtttt cgatcagcgg gccgcccaat gggatgccgg cattgttgaa caccacgtcg
     2461 atccgtccgc cgctggcctt ggcgcaggct gcaagtgcgg catcccaggc cgtccggtcg
     2521 cgcacgtcga gcaggtggac cgaactggct cccgccggca gcatggccgc ggtttcgcgc
     2581 attccggcct cgttgacatc ggctagcccc acgaaccagc cctgcgcccc gaacttgacc
     2641 gcaaccgccc ggccgattcc cgatgccccg ccggtgatga agatgcactt cctgctggcc
     2701 acgacgtccc tcccgaatcc cgcttacact tgtgtcagca acactggcag gggcaaggcg
     2761 accttgcaat cggtcaattc agatcccgag cagcgcgcgc gccgtttccg gccatcctgg
     2821 ggcaaagaac aatccgatgg agccaatctg ggtcgcaaga ataagacccg ctgccggcaa
     2881 aaaggccgga tgcaggcggc cgcgcgtctt gatgtcgtac aaggccatgg ctgtcatcag
     2941 cgcccaggta agcggaaacc ggcccagcgt ttcgcgggcc aggccttgcc ccaggaccga
     3001 tgtcaggtcg cggcccagcc agcgcgacca gccggtgtcg agaatcgcga agaccgccag
     3061 cagcatcagg cgctt
//
LOCUS       DASUDV010000208         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_315996,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000208 DASUDV010000000
VERSION     DASUDV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315996"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFV30_04440"
     CDS             complement(<1..486)
                     /locus_tag="VFV30_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX4847367.1"
                     /translation="MVQGYQFIDINAKGEGIVVAAFDNQVAYCTQAGATVTARVVAAL
                     RDLLERGEPGALLDAIRAWPGAPLADALPLRVAGGIHALHLKGVEPGLAAIYEDRAGV
                     DDAAIVAAAIRAHEADLLPWLDGPPQTNEAGRSSNFIAAMLWLAEQGLPPRFECLEIG
                     SS"
     gene            532..1185
                     /gene="hisG"
                     /locus_tag="VFV30_04445"
     CDS             532..1185
                     /gene="hisG"
                     /locus_tag="VFV30_04445"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX4847368.1"
                     /translation="MSNPLTFAVPKGRILDEALPLMARAGVVPEAGFHDKGNRALSFA
                     CEGSDMRVIRVRAFDVATFVAHGAAQVGIVGSDVVEEFAYPDLYAPVDLAIGHCRLSV
                     AEPVGQAGAPLSSHLRVASKYPNLTRKHFERMGVQAEVVKLNGAMELAPGLGLASRIV
                     DLVSTGRTLKDNGLVETSTILEVSARLIVNRAALKTDPRVGALVEAFRALAAPAEAA"
     gene            1185..2480
                     /gene="hisD"
                     /locus_tag="VFV30_04450"
     CDS             1185..2480
                     /gene="hisD"
                     /locus_tag="VFV30_04450"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX4847369.1"
                     /translation="MLRLNTRDPGFDAAFRKLVSDRRESDENVSRDVQIILDDVRARG
                     DEALIELTRKFDQHDLASEADWQVDAATCREAFDNLTPDLRAALELAAKRIRTFHEGQ
                     KPANRDEQDKHGVRMGARWGAVDAAGLYVPGGRAAYPSSLLMNAIPAKVAGVERLVVV
                     TPSPKGQINPLVLAAAHLGGVDEVWRIGGAQAVGALAYGTERIKPVDVIVGPGNAWVA
                     EAKRQLYGVVGIDMVAGPSEILLIADGQNSPEWTAADLLSQAEHDPSAQSILITDDPV
                     FADTVADRIGVELAMLPTARVASESWNTHGTIIEVADLMTEAPALANALAAEHVQIAT
                     DDPQALFDKIRHAGSVFLGRMTPEAVGDYVAGPNHVLPTGRRARFSSGLSVLDFMKRT
                     SFIQLDAKALKAVGPAAVELANAEGLTAHARSIEVRLGI"
     gene            2477..>2602
                     /locus_tag="VFV30_04455"
     CDS             2477..>2602
                     /locus_tag="VFV30_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX4847370.1"
                     /translation="MSKSKARPAARLAAVQALYQHAMEGTPQASLLDEFHRHRLGA"
BASE COUNT          413 a          920 c          842 g          427 t
ORIGIN      
        1 gctggacccg atttccaggc actcaaagcg cggtggcaga ccctgctcgg ccagccacag
       61 catggcagcg atgaaattgc tggagcggcc agcttcgttg gtctgcggcg ggccgtcgag
      121 ccaggggagc aggtccgcct cgtgcgcccg gatcgctgcg gcaactattg ctgcatcgtc
      181 caccccggcg cgatcctcgt agatcgccgc cagccccggc tccacgcctt tcagatgcag
      241 tgcatggatg ccgcccgcca cccgcagcgg cagggcatcg gccagcggcg cgcccggcca
      301 ggcgcggatc gcatcaagca gcgctccggg ttcgccccgc tccagcaggt cgcgcagcgc
      361 cgccaccacc cgcgcggtga cggtcgcgcc cgcctgcgtg caataggcca cctggttatc
      421 gaacgccgcg acaacgatcc cctcaccctt cgcgttgata tcgatgaact gatacccctg
      481 gaccataggc gctccttgcc ctttcggccc gccttgccta aggcgcgcgc tatgtcgaac
      541 ccgctgacct tcgccgtgcc caagggccgc attctggacg aggcgctgcc actgatggct
      601 cgcgctggtg tggtgccaga ggcgggcttt cacgacaagg gcaaccgcgc cctttccttc
      661 gcctgtgaag gctccgacat gcgggtgatc cgcgtgcgcg cctttgacgt tgcgaccttc
      721 gttgcccacg gcgcggcgca ggtcgggatc gtcggctcgg atgtggtcga ggaattcgcc
      781 taccccgatc tctacgcccc ggtcgacctg gcgatcggcc actgccgcct gtcggttgcc
      841 gaacctgtcg gtcaggccgg cgcaccgctc tccagccacc ttcgcgtcgc cagcaagtat
      901 cccaacctca cccgcaagca ctttgagcgg atgggggttc aggccgaggt cgtgaagctc
      961 aatggggcga tggaactggc ccctggcctg ggcctggcca gccggatcgt tgacctggtt
     1021 tcgaccgggc gcacgctcaa ggacaatggc ctggttgaaa cgagcacgat cctcgaggtt
     1081 tcggcccggc tgatcgtcaa ccgcgctgcg ctgaagaccg atccccgcgt tggcgcgctg
     1141 gtcgaggcgt tccgtgcgct ggccgctccg gcggaggccg cctgatgctg cgcctcaata
     1201 cccgcgatcc cggctttgac gccgccttcc gcaagctggt ttctgatcgg cgcgagagcg
     1261 acgagaatgt cagccgcgat gtccagatca tcctcgacga tgtccgcgcc cgcggcgacg
     1321 aggcgctgat cgagctgacc cgcaagttcg atcagcacga tctcgccagc gaagccgatt
     1381 ggcaggtcga tgccgccacc tgccgcgagg cctttgacaa cctgacgccg gacctgcgcg
     1441 ccgcgctgga actcgccgcc aaacgcatcc gcacgttcca cgaaggccag aaaccagcca
     1501 atcgcgacga gcaggacaag catggcgtgc gcatgggtgc gcgctggggt gcggtcgatg
     1561 cggccgggct ctatgttccg ggcggacgcg cggcttaccc atcctcgctg ctgatgaacg
     1621 caatcccggc caaggttgcc ggggttgagc ggctcgtcgt cgtgaccccc agccccaagg
     1681 gccagatcaa cccgctggtc ctcgccgccg cgcacctggg cggtgtcgat gaagtctggc
     1741 ggatcggcgg ggcccaggcg gtcggcgcgc tggcttatgg gaccgagcgg atcaagccgg
     1801 tcgacgtcat agtcggcccc ggcaatgcat gggtcgccga agccaagcgt cagctttatg
     1861 gcgtggtcgg gatcgacatg gtcgccgggc caagcgagat cctgctgatt gctgacgggc
     1921 agaactcgcc cgaatggacc gccgccgacc tgctcagcca ggccgagcac gatccttcgg
     1981 cccagtcgat cctgatcacc gatgacccgg tcttcgccga tacggtggcg gatcggatcg
     2041 gcgtcgaact cgccatgctg cccacggcgc gcgttgccag cgaaagctgg aacacccacg
     2101 gcacgatcat cgaagtggcc gacctgatga ccgaggcgcc cgccctcgcc aatgccctcg
     2161 ccgccgagca cgtccagatc gcgaccgacg atccgcaagc gctgtttgac aagatccgcc
     2221 acgccggttc ggttttcctg ggccggatga cgcccgaagc cgtgggcgac tatgttgccg
     2281 gccccaacca cgtgctgccc accgggcgcc gcgcgcggtt ctcgtccggg ctgtcggtgc
     2341 ttgatttcat gaagcgcacc agcttcatcc agcttgatgc caaggcgctg aaggccgtag
     2401 gccccgccgc ggtggagctg gccaatgccg agggcctcac cgcccatgcg cgctcgatcg
     2461 aagtgagact gggaatatga gcaagtccaa ggcccgcccc gccgcccgtc tcgcggcggt
     2521 tcaggcgctg taccagcacg cgatggaagg cacgccgcag gcctcgctgc tcgacgagtt
     2581 ccatcgccat cgcctcggcg cg
//
LOCUS       DASUDV010000209         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1177435,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000209 DASUDV010000000
VERSION     DASUDV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177435"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFV30_04460"
     CDS             complement(<1..1286)
                     /locus_tag="VFV30_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX4847371.1"
                     /translation="MSGDGRLYTLTSEQQPAVDPGEVVWLSASAGTGKTQVLSARVLR
                     LLLQQDVRPDQILCLTFTKAGASEMATRINERLARWVRARDEELARDLKAIGADYGIE
                     TLARARTLFAQVLDCPGGGLRIDTIHAFAQWLLSAFPHEAGLTPGTKAMEDRDRDLLL
                     REVLADLLLEAERSNDAATLATLERLSIRSGPEEVERFLLRCADAHEVWEGAGGWQPP
                     MLPRVKQLLGLHSDASHADLAAMCGDEVFPTEALRCCLAAYAQWNGKRGTAGRAVIAD
                     WLGSDSSARLDRLVELHAALFTQKDTVRDVSSLAKFEEHFEQAAEAVLQASLAIRDRR
                     ALLDLAEWLAPALELGWRFAGKWAEAKARAGFIDFNDQIRQAARLLNRSELSEWIRYK
                     LDRRFDHVLVDEAQDTNVAQWQIINALIGDFFAGEGA"
     gene            complement(1283..>3623)
                     /gene="addB"
                     /locus_tag="VFV30_04465"
     CDS             complement(1283..>3623)
                     /gene="addB"
                     /locus_tag="VFV30_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEX4847372.1"
                     /translation="LPIVAAGVTSASPALANLLSVVAHLPRGAVILPDLDLSLSDAVW
                     DMLGSAGNGDPPFGEYDAPAHPQYHLKLLLNRMNLSRKEVQAWHRAGMSAAPPERSRA
                     ISNLFLPAQASGGWFDLPAAQRRLSGVRLMECAHPGEEAQAIAILMRETLETPERRVA
                     LITPDRALAGRVVAHLRRWGITADDTAGQPLSQTTAGRLVLLLAEVAAQQAAPVPLLA
                     LLEHPWVRSGEARAGWLDHARALDLALRGPRPAPGLPPLRVAVVRLAKRLPSIQVWWE
                     ELETLLGPLLEAEGRQPRFSLLDQLARVGEALCGNTLWGNADGRALAALVEELREAAG
                     EAQLMLDRADLPAVLRDAMDRVAVRPPWGGHPRVAIYGLLEARMSRADLVICGGLTEG
                     SWPASQAPDPLLPPAVLRALGVPGAEFRTGLAAHDLAGALGAPEVVLSWSQRDEGGPV
                     IPSRFVLRVQALLGELAPAHAEQHAVELARTIDLGPKVAPHLRPAPEPTLAQRDVSLR
                     VTAIDRLLGDPYQFYAQAILRLPKLDTLDAEPSAAWKGTVIHKVLERWHKAGGRSGEL
                     LALASEELAAMQAHPLVRTLWWPRLARALEWIDGEVARLQAEEGRSVLESEIRGEMTF
                     AGVKVHGRADRIDRLPDGSLAIVDYKTGKPPSRAMVADGNSLQLGVLGLIAREHGFAG
                     VAGDPEQFEYWSFGRDRKRGEEGFGYRFKPFRTEKAAGISEEEFLESTEDYLARAIAA
                     YIKGEAPFEASLQPDLLGYNDYDQLMRLDEWQARGSDKP"
BASE COUNT          624 a         1299 c         1140 g          560 t
ORIGIN      
        1 gcgccctcgc cggcgaagaa atcgccgatc agcgcattga ttatctgcca ctgcgcgaca
       61 ttggtatcct gggcctcgtc gaccagcaca tgatcgaagc gccggtccag cttgtagcgg
      121 atccattcgg acaattcgct gcggttaagc aaccgcgccg cctggcggat ctgatcgttg
      181 aaatcgatga acccggcgcg cgccttggct tcagcccact tgcccgcgaa acgccagccc
      241 aattcgagtg cgggggcgag ccattcggcc aggtccagca gggcacgccg atcgcggatg
      301 gccagcgatg cctgcagcac tgcttcggcg gcctgttcga aatgctcctc gaacttggca
      361 aggctggaca cgtcacgcac ggtgtccttc tgggtgaaca gcgccgcatg caattcgacc
      421 agacggtcaa gccgcgccga agagtcgctg cccaaccagt cagcgatgac cgcgcggcca
      481 gccgtccccc tcttgccgtt ccattgcgca taggcagcaa gacagcaacg cagcgcctct
      541 gtcgggaaca cctcgtcccc gcacatcgcc gcgagatcgg catggctggc atcgctatgc
      601 aaaccgagca gctgcttgac ccgcggcagc atcggcggtt gccagcctcc cgccccttcc
      661 cacacttcgt gcgcatcggc acagcgcagc agaaaccgct cgacctcctc ggggcccgaa
      721 cggatcgaga gccgttcgag tgtggccagc gttgccgcat cgttgctgcg ttcggcctcg
      781 agcaacaggt cggcgagaac ctcgcgcagc agcaggtcgc gatcgcgatc ttccatcgcc
      841 ttggttccag gggtcagccc ggcctcgtgc ggaaaggccg aaagcagcca ctgggcaaag
      901 gcatggatcg tatcgatcct gaggcccccg cccggacagt cgagcacctg tgcgaacagg
      961 gtgcgggcgc gggccagggt ttcgatccca taatcggcgc cgatggcctt cagatccctg
     1021 gccagctcct cgtccctggc ccgaacccat cgcgccaggc gttcgttgat gcgggtagcc
     1081 atttcgctgg ccccggcctt ggtaaaggtc aggcacagga tctggtcagg tctgacatcc
     1141 tgctgcagca gcaggcgcag cacccgggcc gaaagcacct gggtcttgcc ggtaccagcc
     1201 gaagccgaaa gccagaccac ctcacccgga tcgaccgcgg gctgctgctc ggaggtgagc
     1261 gtgtagagcc ggccatcacc gctcatggct tgtcgctccc gcgcgcctgc cactcgtcaa
     1321 gccgcatcag ctgatcgtaa tcattatagc ccagcagatc gggctgaagg ctggcttcga
     1381 acggcgcttc acccttgatg taagccgcga ttgcccgggc aagatagtcc tcggtacttt
     1441 ccaggaactc ctcttcgctg atcccggccg ccttctcggt ccggaagggc ttgaagcgat
     1501 atccgaaacc ctcttcgccg cgtttcctgt cgcgcccgaa cgaccagtat tcgaactgtt
     1561 cgggatcacc ggcaacaccg gcaaagccat gttcgcgcgc gatcaggccg agcaccccaa
     1621 gctgcagcga attgccatcg gccaccattg cgcggctggg cggcttgccg gtcttgtaat
     1681 cgacgatcgc caggctcccg tcgggcagac ggtcgatccg gtcggcgcgg ccatggacct
     1741 tgaccccggc aaaggtcatc tcgccgcgga tttcgctttc aagaacactt cggccttcct
     1801 cggcctgcaa ccgcgccact tcgccatcga tccattccag cgcacgggct agccgcggcc
     1861 accaaagggt gcgaaccagg ggatgcgcct gcatggcggc cagttcctcg ctggccagcg
     1921 cgagcagctc acccgaacgc ccgccggcct tgtgccagcg ctccagcacc ttgtggatca
     1981 cggtgccctt ccacgccgcg ctaggctcgg catcgagcgt atcaagcttg ggcagccgca
     2041 ggatcgcctg ggcatagaac tggtagggat cgccgagcag ccggtcgatc gcggtaaccc
     2101 tcagcgagac atcgcgctgc gccagggtgg gctcgggcgc cggccgcagg tgcggtgcaa
     2161 ccttcggccc cagatcgatc gtgcgggcaa gttcgacggc atgctgttcg gcatgagccg
     2221 gtgccagttc gcccagcagt gcctggaccc gcaggacaaa gcggctcggg atcaccggac
     2281 cgccctcgtc gcgctgcgac cagctcagca ccacctcggg ggcgcccagc gcgccagcca
     2341 gatcgtgcgc ggcaagcccg gtgcggaact ccgccccggg cacccccagc gcccgcagta
     2401 ctgccggcgg cagcagcgga tcgggcgcct ggctggcagg ccatgaccct tcggtcagcc
     2461 cgccgcagat caccagatcg gcccggctca ttcgtgcttc aagcaggccg tagattgcga
     2521 cccgcgggtg cccgccccag ggcggacgca ccgccacccg gtccattgcg tcgcgcagca
     2581 ccgcgggcag atcggcgcgg tcaagcatca gctgcgcttc gcccgcggcc tcgcgcagtt
     2641 cctcgaccag cgccgccagc gcccggccat cggcattacc ccacaaggtg ttgccgcaca
     2701 gcgcttcgcc cacccgggcc aattgatcga gcaggctgaa tcgaggttgg cggccctccg
     2761 cttccagcag aggcccgagc aaggtttcga gctcctccca ccagacctgg atagatggca
     2821 ggcgcttggc cagtcgaacg accgccaccc tgagcggcgg caagcccggt gctgggcgcg
     2881 gtccgcgcag cgccagatcg agagcacggg catgatcgag ccagccagca cgcgcctcgc
     2941 ccgagcggac ccagggatgc tccagcagcg ccagcagcgg caccggtgcg gcctgctgtg
     3001 ccgccacttc ggccagcagc agcaccagac gccccgccgt ggtctgcgaa agcggctggc
     3061 cggcggtatc gtctgcggtg atgccccacc gccgcagatg cgccactacc cgcccggcca
     3121 gggcgcgatc gggcgtgatc agggcaaccc ggcgctcagg cgtctcaagc gtctcgcgca
     3181 tcaggatcgc gatggcctgc gcttcctcgc caggatgggc gcattccatc aatcgcaccc
     3241 cggaaaggcg gcgctgcgcc gcaggcaggt cgaaccaccc gccgctcgcc tgcgccggca
     3301 ggaacaggtt ggagatcgcc cggctgcgct caggcggcgc ggcagacatg cctgcccggt
     3361 gccaggcctg cacttccttg cgcgacaggt tcatccggtt gagcagcagc ttgagatgat
     3421 actgcggatg agccggcgcg tcatattcgc caaacggcgg atcgccgttc cccgccgaac
     3481 ccagcatgtc ccagaccgcg tcgctcagcg aaaggtcaag atcgggcagg atcaccgcgc
     3541 cgcgcggcag atgtgccacc acgctgagca gatttgccag cgccggcgag gcactggtga
     3601 ccccggccgc gacaatcggc agc
//
LOCUS       DASUDV010000210        10930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_94978,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000210 DASUDV010000000
VERSION     DASUDV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94978"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFV30_04470"
     CDS             complement(<1..507)
                     /locus_tag="VFV30_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX4847373.1"
                     /translation="MTLLAPSRLIAAGLAAALAAAAATAQPAATPAAPAAQPAKPAAT
                     EPHRQPKGYVLVFADEFDGTGLPDPAKWAYDTHRNQQGWYNQEKQYYSAARSENSRLE
                     NGRLVIEARAETLDRARFPDWGGQSYTSARLFTQGRAAWTYGFFEIRAKLPCGVGTWP
                     AIWMLPEDP"
     gene            complement(598..3300)
                     /locus_tag="VFV30_04475"
     CDS             complement(598..3300)
                     /locus_tag="VFV30_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847374.1"
                     /translation="MRKSAVVLALGVSLGALAAPAYAQDAPPAGEPAKEETAKEETGR
                     GDIVVTAERREQNLQDYGGTAASFQGDELRKRGIQDMTDMNDVIPGLTVANNGGNVEI
                     WIRGVGSSNNTELGDPAAAFHYDGVYVPRPAGIGSAFFDIERVEVNFGPQGTLRGRNA
                     TAGSVNAIAWKPGLGVWDAELEAEYGNYDQKSVRGMVNVPLGEKAALRVSGMMLSHDS
                     YYENVGPVGNIDVAEAEDNAAFRVQLLVKPSDRLSILVAGDYIHETGTGYTGTNYANP
                     LGNGIDPDDIEDPRKVFARAFTPDLDTKHWGVRTVITYEADPFTIEYTGSFRRMKYDY
                     DAVTPIAPAYPGLLANLSALPGTPEEEFDNWSRFQFITDSKSHYHELRFFNNKGPVIW
                     SLGGLYFKEDQYAFLGSAADRNLFFSGAEFNMPDVDAESWALYGDMTWEVNDRFRLTA
                     GIRYTDDSKSREGIAARYGFALGDGNFNCCGGVRVGTEGFQFAGKGRTIFNPDTDNNG
                     TVSDQEILNFYFNGVKQFGTRDNVDNIFANGPIPGHFGFVPGVPTCEDTITGDFWTCQ
                     GFEPSGQFTYGVPFAGQIFRQTGSMKDNFVDWRLRAEYDISDDSMVYALVATGHKSGG
                     FNDNIGDAGVAPTYGTERVRLYELGSKNVFWLGSVKTKLNVSAFYNDYDDQVFTSLLS
                     VSQIVDFNNQLGNNIPVPPGTNTALVISYSYNAADSEIYGINVEGGFELPSNLNLSFN
                     ALWLEAKIKNSQPIQDFRFQSDVAPSEAVFQSIDGKRLPRTPRWQLNASLSQKIDVGS
                     GSIDWVISGGYRSSQFMTIFNSQDFQNPNAPRLRLNDTVGGYWTFDLGAGYTHGADGK
                     LRFEVFANNVTNAVHEAAIIITQFDNTRFFTRPRTFGGRVRIKF"
     gene            3437..4483
                     /locus_tag="VFV30_04480"
     CDS             3437..4483
                     /locus_tag="VFV30_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4847375.1"
                     /translation="MAKRGPITIEDVAEAAGVSRQTVSRVINLSPNVKTAVRERVNAA
                     IERLGYVPNLSARRMGGGRSYLILAINDRQRTLENWQAGRGNDWVDQMLYGGMTECER
                     HGYHMVFELIDANGEASARQLANVITALRPDGVILTPPHCDNAAMLAVLAEHKVPCAR
                     VGHREGAASVDVHMDEEGAAEAATAHLLALGHRRIAYVAGAPNYGNSAQRVEGYRRAL
                     AAAGLTVDPALIGNGDFHFDTAAAVIAAMLAGPNRPTAVIADNDEMAFAALHEASRHG
                     LAVPAGLSVLSFEDTPGVRFSVPPLTAIRQPTAAMIATASARLIALSKGEDGYGSYQL
                     PFDLVVRTSTGQPG"
     gene            4480..5721
                     /locus_tag="VFV30_04485"
     CDS             4480..5721
                     /locus_tag="VFV30_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847376.1"
                     /translation="MTQQQTEPTGTGLVRYGAAVVGLNLAYMPLLVLLLPRRAAELFG
                     AEAAGVVSWLMLAGAVAASLGNIAAGRFADWWIGRFGSRRGAIALGLAALLATYPALA
                     LAETVPALVLAVIAFQLALNLALSPAMAVLADHVPDARKGPVSGWLGAAVPLSALGTA
                     GIGWALPAQGNSAFLVLAGLVLLLVLPLPLFWGLGRRAAVPAAAPSASAPAAPRAVDS
                     RVWRLWAARLMVQLANCFLLFYLFLYLESLIAARPDWHGRDPSDTLADLSLIGAVCAV
                     LGALACGRLARHAAPRGRLLAGAALAIAAGLFAIGSAPSPLAMLAGFALIQLALAGYL
                     AIDMAWVAQLLSDHPRRGQVLGLINLSNTLPGVAAAVLTLQGLQHGGLGDTLELAYRL
                     CALGALLAAALTLTLRGNPAK"
     gene            complement(5791..6468)
                     /locus_tag="VFV30_04490"
     CDS             complement(5791..6468)
                     /locus_tag="VFV30_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX4847377.1"
                     /translation="MTRRTLAALAAAASALALAAPAQAGSSEGKIQIKAFATGVLPNG
                     EITEVRTNLVGAPATSDSQASDSVVPTLAVEYFLAPNVSVETICCVTPHDVTGKGGLA
                     GASLVNDAIILPATVTLKYHFDLGAVKPYIGAGPTRFFIFSEGVGADAALLGATRVDL
                     SDSFGVALQAGFDMPLNDKGLGFSVDAKRYFVGTTASFFAGNTLALQTQHKLDPWVVS
                     GGLSYRF"
     gene            complement(6593..7183)
                     /locus_tag="VFV30_04495"
     CDS             complement(6593..7183)
                     /locus_tag="VFV30_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847378.1"
                     /translation="MKSFKIRSLAFAAALPLAAIATPAVAHDHGAAKATAVYAPITQA
                     EVEAAQTAWCGALVAIATEHDTKGQPAAKELAKKVLDSAYGYGMGPVLFKPTLTVAPQ
                     TFRTTYDGALAYFVGGDKAYPTDTGFALKGWRKCETSNTTIVITGNSALSMGNVTMWD
                     SKGNMTRVDKTWGYVRGPDGQLKIVLHHSSLPYTAK"
     gene            7349..8401
                     /locus_tag="VFV30_04500"
     CDS             7349..8401
                     /locus_tag="VFV30_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4847379.1"
                     /translation="MQSPQPALVPAVETVAELRELYRAAEARAARLRLLSTNGRQLTM
                     AESATIEAILQGCADRLGYFLGRRGGSVQPAAAAGLPIPAPGSDGRTLAVLAIEGLAS
                     LDDVADAEDREAVQLQLEMIGATIDRIERARDLQEREARLEFVVGRLFSAQEEERRRV
                     SHELHDGVAQTATALARLLEGAESSAERTKLAGIARELVGELRAVIRGLRPTVLDDLG
                     LEAGLRALAEALEAEGFAVRIVLASDVDRWPGHIETALYRVAQEALSNVRKHAGGPCR
                     VTLELAARRSDERPYLRIADEGQGPQRPAGEDPARSHFGIDVMRERMVSIGGALDWQA
                     SPQGGVTVLASFPKVV"
     gene            8404..9039
                     /locus_tag="VFV30_04505"
     CDS             8404..9039
                     /locus_tag="VFV30_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4847380.1"
                     /translation="MAVRILIVDDHQLAREGLKAVLSDDGLAVVGEAASGEEAVALVS
                     QLVPDVVLMDVRLGPGIDGLEATRRIAALGLPSRVIMLSLHDMPVYVREALAAGAAGY
                     VLKDASIAELRGAVSQVMAGQSVIPLGLINAALRDSEREVRPAEVLAALTGREREVLD
                     LVAEGLTNKEIARRLAVSPATVKAHVERIIAKLGVTDRTQAAVLVARADPA"
     gene            9036..>10930
                     /locus_tag="VFV30_04510"
     CDS             9036..>10930
                     /locus_tag="VFV30_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847381.1"
                     /translation="MNQPVLSRKMTRFWADRRLALKGLVVIALPLAILLGALVSLYIA
                     SNAEARAENDVRRAFAIQRDTYQVHALLAEAAAGVRGYALTGQERFLEPYRKAEADLP
                     LTMQRLDDAIRDGEVRQHFNRINDLTARKREGLKGIVELARQGDRAAIERALVANKIV
                     LDAMRREIDLIQRREGVLLDQRRARVDEVRDRFLLLTAISAVIGLLGSLAAVYLFSTG
                     IVRRVQMLEANAELLARGEALAEMPDDADEIGRLAQRMARASALLRGREQALRESEER
                     FRLVIEEVRDYGIFALDPQGVVTSWNLGAERIKGWEAEEILGQHFSRFYPEETRGHLP
                     AEMLDRARRDGTAEDEGWRLRKDGSPFWANVVITALHDEHGALRGFAKVTRDMTERRR
                     SEEALRFANEEAIAANLAKSEFLSRTSHELRTPMSAILGFGQLLELDEDQFEPRHREA
                     LSQIMKAGRHLLSLINDLLDISSIEAGGAELVLEPVDLGEVLQEVHGLAAPIVAAAGL
                     RFELEPPAEPLVAWADRRRVVQVMLNLISNAAKYNRTGTLVRIACLPRGRDVRVEVED
                     DGEGVVSSEASRLFTPFDRLGQQNRAKVDGTGLGLALSKRLIESMGGQIGHEAPPQGA
                     RFWFTLPT"
BASE COUNT         1637 a         3617 c         3889 g         1787 t
ORIGIN      
        1 cgggtcttcg ggcagcatcc agatcgccgg ccaggtgccg accccgcagg gcagcttggc
       61 gcggatttcg aagaagccat aggtccaggc ggcgcggccc tgggtgaaca gccgggccga
      121 ggtgtagctt tgcccgcccc aatcggggaa gcgggcgcgg tccagcgttt cggcgcgggc
      181 ctcaatcacc aggcggccgt tttccagccg ggaattctcg ctccgcgcgg ccgaatagta
      241 ctgcttttcc tggttgtacc agccctgctg gttgcggtgg gtgtcatagg cccacttggc
      301 cggatcgggc aggccggtgc cgtcgaactc gtcggcaaag accaggacat agcccttggg
      361 ctggcggtga ggttcggtgg ctgccggttt ggccggctgc gcggcgggtg ccgctggcgt
      421 ggcggccggc tgagccgttg ccgccgcagc ggccagcgcc gcggcaagac cggcggcgat
      481 cagtcgggag ggggcaagca gggtcatcgc accgttccgg gcctgagggg agggaggaaa
      541 ggcattggcg caggcgcccg gccgtccgtc aagcggggcc gggcgcctgg ccgcagatca
      601 gaacttgatc cggacccggc cgccgaaggt gcgcggacgg gtgaagaagc gcgtgttgtc
      661 aaactgggtg atgatgatcg ccgcttcgtg caccgcgttg gtgacgttgt tggcgaacac
      721 ttcaaaccgc agcttgccat cggcgccgtg ggtgtaaccg gcccccagat cgaaggtcca
      781 gtaaccgccc accgtatcgt tgagccgcag gcgcggggcg ttcgggttct ggaagtcctg
      841 gctgttgaag atcgtcatga actgcgacga gcggtaaccg ccagagatga cccagtcgat
      901 gctgcccgaa ccgacgtcga tcttctgcga gagcgaggcg ttcagctgcc agcgcggggt
      961 gcgcggcagg cgcttgccgt cgatcgactg gaacaccgcc tcggacggcg cgacgtccga
     1021 ctggaagcgg aagtcctgga tcggctgcga gttcttgatc ttcgcttcga gccacagggc
     1081 gttgaagctg aggttcaggt tcgaaggcag ttcgaagccg ccttcgacgt tgatcccgta
     1141 gatttccgaa tccgccgcgt tgtagctgta cgaaatcacc agcgcggtgt tggtgcccgg
     1201 gggaaccggg atgttgttgc ccagctggtt gttgaaatcg acgatctgcg aaaccgacag
     1261 cagcgaagtg aagacctggt cgtcgtagtc gttgtagaac gccgagacgt tgagcttggt
     1321 cttcacgctg cccagccaga acacgttctt gctgcccagt tcgtacaggc gaacccgttc
     1381 ggtgccatag gtcggggcaa cgccggcatc gccgatgttg tcgttgaacc cgcccgactt
     1441 gtggccggtg gcgaccagcg catagaccat gctgtcatcg ctgatgtcgt attccgcgcg
     1501 caggcgccaa tcgacgaagt tgtccttcat cgacccggtc tggcggaaga tctgccccgc
     1561 gaacggcacg ccataggtga actggcccga gggttcgaag ccctggcagg tccagaagtc
     1621 accggtgatc gtgtcttcgc aggtcggcac gcccggcacg aagccgaagt ggcccgggat
     1681 cggcccgttg gcgaagatgt tgtcgacgtt gtcgcgggtg ccgaactgct tcacgccgtt
     1741 gaagtagaag ttcaggattt cctggtcgct gaccgtcccg ttgttgtccg tatcgggatt
     1801 gaagatggtc cggcccttgc ccgcgaactg gaacccttcg gtgccgacgc gcacgccgcc
     1861 gcagcagttg aagttgccgt cgcccagcgc aaagccatag cgcgccgcga tcccttcgcg
     1921 cgacttgctg tcgtcggtgt agcggatccc ggcggtcagg cggaagcggt cgttcacttc
     1981 ccaggtcatg tcgccgtaaa gcgcccagct ttcggcatcg acatcgggca tgttgaattc
     2041 cgcgcccgag aagaacaggt tgcggtccgc ggccgagccg aggaaggcgt actggtcttc
     2101 cttgaagtag aggccgccca ggctccagat caccgggccc ttgttgttga agaaccgcag
     2161 ctcgtggtag tgcgacttgg agtccgtgat gaactggaag cgcgaccagt tgtcgaactc
     2221 ttcctcaggc gttcccggca gggccgaaag gttggcgagc agccccggat aggccggcgc
     2281 gatcggcgtc accgcatcgt aatcgtactt catccggcgg aagctgccgg tgtattcgat
     2341 cgtgaacgga tcggcctcat aggtgatgac cgtgcgcacg ccccagtgct tggtgtcgag
     2401 atcgggagta aaggcgcggg caaagacctt gcgcggatcc tcgatgtcgt ccggatcgat
     2461 gccattgccc agcgggttgg catagttggt gccggtgtag ccggtgccgg tttcgtggat
     2521 gtagtcgccc gccaccagga tgctcaggcg gtcgctcggc ttgaccagca gctggacgcg
     2581 gaaggcggcg ttgtcttcgg cttcggcgac gtcgatgttg ccgaccggcc cgacgttttc
     2641 ataatagctg tcgtgcgaga gcatcatgcc cgacacgcgc agcgcggcct tttcgcccag
     2701 cggcacgttg accatcccgc gcaccgactt ctggtcgtag ttgccgtatt cggcttccag
     2761 ttcggcgtcc cacacgccca gacccggctt ccacgcaatc gcgttgaccg aaccggcggt
     2821 ggcgttgcgg ccgcgcaggg tgccttgcgg gccgaagttc acttcgacgc gctcgatatc
     2881 gaagaaggcc gagccgatcc cggccgggcg cggcacgtag acgccgtcat agtggaaggc
     2941 cgcggccgga tcgcccagtt cggtgttgtt gctggagccg acgccgcgga tccagatttc
     3001 aacgttgccg ccgttgttgg caacggtcag gcccgggatg acgtcgttca tgtccgtcat
     3061 gtcctggatg ccgcgcttgc gcagctcgtc accctggaag ctggcagccg tgccgccgta
     3121 atcctgcagg ttctgttcgc ggcgttccgc cgtgaccacg atatccccgc gcccggtttc
     3181 ttccttggcg gtttcttcct tggcgggctc gccagccggc ggcgcatcct gcgcataggc
     3241 cggagcggcc agcgccccca gcgagacacc aagggcaagc acgacagcag atttacgcat
     3301 cagatcctcc ccatgcggtc gcgatgcgcg actcgtcacg ttctagatac tcattttgtg
     3361 agcgctcaca actgaaaaat gagagcgctc acaaaatggc tggattggca atcgatttcg
     3421 gctagggttc gcagccatgg cgaagcgggg acccatcacg atcgaagacg tggccgaagc
     3481 ggcgggcgtg tcccggcaga ccgtttcgcg cgtcatcaac ctcagcccca acgtcaagac
     3541 cgcggtgcgc gagcgggtga acgccgcgat cgagcggctg ggctatgtcc ccaacctgtc
     3601 ggcccggcgg atgggcggcg ggcggtccta tctgatcctg gcgatcaacg accggcagcg
     3661 caccctggaa aactggcagg ccgggcgcgg caacgactgg gtcgaccaga tgctctacgg
     3721 ggggatgacc gagtgcgagc gccacggcta tcacatggtg ttcgaactga tcgacgccaa
     3781 cggcgaggca tcggcccggc agctagccaa cgtgatcacc gcactgcgcc ccgacggcgt
     3841 aatcctgacc ccgccgcact gcgacaacgc ggcgatgctg gcggtgctgg ccgaacacaa
     3901 ggtgccctgc gcccgggtcg gccaccgcga gggcgcagcc agcgttgacg tccacatgga
     3961 cgaggaaggc gcggccgaag ctgccacggc ccacctcctc gcgctgggcc accgccggat
     4021 cgcctatgtc gccggcgcgc ccaattacgg caactcggcc cagcgggtcg aaggctaccg
     4081 ccgcgccctc gcagcggccg gactgacggt cgatccggcg ctgatcggca acggcgattt
     4141 ccattttgac accgccgccg ccgtgatcgc cgccatgctc gccggcccca accggccgac
     4201 cgcagtgatc gccgacaacg acgaaatggc ctttgccgcg ctgcacgagg cgagccggca
     4261 cggcctggcg gtgccggctg gcctttcggt cctcagcttt gaagacacgc ccggcgtgcg
     4321 cttctcggtc ccgccgctga ccgcgatccg ccagccgacc gcggcgatga tcgccacggc
     4381 cagcgcccgg ctgattgcgc tgagcaaggg cgaggacggc tatggcagct atcagctgcc
     4441 gttcgatctg gtggtccgga ccagcaccgg ccagcccggg tgacccagca gcagacggag
     4501 ccgaccggca cggggctggt ccgctatggc gcggcggttg tcgggctgaa cctggcctat
     4561 atgccgctgc tggtgctgct gctgccgcgc cgtgccgccg aactgttcgg agccgaggcc
     4621 gccggggtgg tcagctggct gatgctggcc ggcgcggtgg ccgccagcct gggcaacatt
     4681 gccgccgggc ggtttgccga ttggtggatc ggccgctttg gatcgcggcg cggggcgatc
     4741 gcgctgggcc tggccgccct gctggcgacc taccccgccc tcgccctggc cgagaccgtg
     4801 ccggccctgg tcctggccgt gatcgcgttc cagctcgcgc tcaacctggc actgtcgccg
     4861 gccatggcgg tgctggccga tcacgtgccc gacgcgcgca agggcccggt atcgggctgg
     4921 ctgggcgcgg ccgttccgct ctccgcgctc ggcactgccg ggatcggctg ggccctgccc
     4981 gcgcagggga acagcgcctt cctggtcctg gccgggctgg tcctgctgct ggtcctgccg
     5041 ctgccgctgt tctggggcct gggccggcgc gccgccgttc ccgccgctgc gccgtccgct
     5101 tccgcaccgg ctgcgccgcg cgcggtggac tcgcgggtct ggcggctgtg ggcagcgcgg
     5161 ctgatggtcc agttggccaa ctgtttcctg ctgttctacc tgttcctcta ccttgaaagc
     5221 ctgatcgcgg cgcggccgga ctggcacggg cgcgatccct cggacacgct ggccgacctg
     5281 tcgctgatcg gcgcggtttg cgcggttctg ggcgcgctcg cctgcggccg gctggcccgc
     5341 cacgccgcgc cgcgcgggcg gctgctggcc ggtgccgcgc tggccatcgc cgccgggctg
     5401 ttcgccatcg gcagcgcccc aagcccgctg gccatgctgg ccggatttgc gctgatccag
     5461 ctggcgctgg ccggctatct tgcgatcgac atggcctggg tcgcccagct gctgagcgat
     5521 catccccgcc ggggacaggt gctgggcctg atcaacctgt caaacaccct gcccggcgtc
     5581 gccgccgcgg tcctgaccct gcagggcctg cagcacggcg ggctgggcga tacgctggag
     5641 ctggcctacc ggctctgcgc gctgggcgcg ctgctcgccg cggcgctcac cctgacgctg
     5701 cgcggcaatc ccgcaaagtg aaaggggcgg tgcggccgcc tgccggcgtc ccgcaccacc
     5761 ccaatcatcc gcttgccgga acgggccgcg tcagaagcgg tagctgaggc cgccgctcac
     5821 cacccacgga tccagcttgt gctgggtctg gagcgccagg gtgttgccgg caaagaagct
     5881 ggcggtggtg ccgacgaagt agcgcttggc atcgacgctg aagcccaggc ccttgtcgtt
     5941 gagcggcatg tcgaacccgg cctgcagcgc cacgccgaag ctgtccgaca ggtccacgcg
     6001 ggtcgcgccc agcagggccg catcggcgcc cacgccttcg ctgaagatga agaagcgggt
     6061 cgggccagcg ccgatgtagg gcttcaccgc acccagatcg aagtggtact tcagcgtcac
     6121 cgtggcgggc aggatgatcg cgtcgttgac cagcgaggcg ccggccaggc cgcccttgcc
     6181 ggtcacatcg tgcggcgtga cgcagcagat cgtctcgacc gagacgttcg gcgccaggaa
     6241 gtattcgacc gccagggtcg ggaccaccga atcgctggcc tggctgtcgc tggtggccgg
     6301 cgcgccgacc aggttggtgc gcacttcggt gatttcgccg ttgggcagaa cgccggtggc
     6361 gaaggccttg atctggatct tgccttcgga gctgccggcc tgggcgggcg cggcaagggc
     6421 aagggcgctg gcggcggcgg caagcgccgc aagagtgcgt cgggtcatgg ggaaaatctc
     6481 gctgttggga gggatggaga ttggattgcc gggttgcgca cccggcgggc gatctggcgc
     6541 gaacaggcgg ctgccaggag ggggatgaca gccgcctgcc gccaatcttc gcttacttgg
     6601 ccgtgtaggg cagcgaagag tggtgaagga cgatcttgag ctggccatcg gggccgcgga
     6661 catagcccca ggtcttgtcg acgcgggtca tgttgccctt ggaatcccac atcgtgacat
     6721 tgcccatcga cagcgccgaa ttgccggtga tgacgattgt ggtgttgctg gtttcgcact
     6781 tgcgccagcc cttcagggca aagccggtgt cggtcggata ggccttgtcg ccgccgacga
     6841 agtaggccag cgcgccgtca taggtggtgc ggaaggtctg cggagcgacc gtcagggtcg
     6901 gcttgaacag caccggaccc atgccatagc cataggcgct gtcgagcacc ttcttggcca
     6961 gttccttggc ggccggctgg cccttggtgt cgtgctcggt cgcgatcgcc accagcgcgc
     7021 cgcaccaggc ggtctgcgcg gcttcgactt cggcctgggt gatcggggca tagaccgcgg
     7081 tggccttggc ggcgccgtga tcgtgggcaa cggccggggt ggcgatcgcg gccagcggca
     7141 gcgctgcggc aaacgccagg ctgcggatct tgaagctctt catctcactg tctcctgatt
     7201 gcgaaatccg atcgcccagc tggcaccagc ccggcgtctg ccccgatcta ggccccgcac
     7261 aggtgcgccg gaattggccg catggcgggc cgcgcggcct aggccggatg gcctatgcac
     7321 tttggcggga tcgtcctaga gagggccgat gcagagcccc caaccggccc ttgtccccgc
     7381 ggtggaaacc gttgcggaac tgcgcgagct ttaccgcgcc gccgaggcgc gggccgcgcg
     7441 gctgcgcctg ctgtccacca acggccgcca gctgaccatg gccgaaagcg cgacgatcga
     7501 ggcgatcctg cagggctgcg ccgaccggct gggctatttc ctgggccggc gcggcgggtc
     7561 ggtccagccc gcggctgccg ccggcctgcc gatcccggcg ccgggcagcg acgggcggac
     7621 tctggcggtc ctggcgatcg aggggctggc gagccttgac gacgtggccg atgccgaaga
     7681 ccgcgaggcc gtgcagcttc agctcgagat gatcggcgcg acaatcgacc ggatcgagcg
     7741 cgcccgtgat ctgcaggagc gcgaggcccg gctggaattc gtcgtcggcc ggttgttctc
     7801 cgcccaggag gaagagcgcc gccgggtcag ccacgaattg cacgacggcg tggcccagac
     7861 cgcgaccgcg ctggcccgcc tgctcgaagg agccgaaagc agcgccgagc gcaccaagct
     7921 ggccggtatc gcgcgcgagc tggtgggcga actgcgcgcc gtgatccgcg gcctgcgccc
     7981 cacggtgctg gacgaccttg gcctcgaagc cggcctgcgc gcgctggccg aagcgctgga
     8041 ggccgaaggc tttgccgtgc ggatcgtcct tgccagcgac gtggaccgct ggcccggcca
     8101 catcgagacc gcgctgtacc gggttgcgca ggaggcgctc agcaatgtcc gcaagcacgc
     8161 cggcgggccg tgccgggtga cgctggaact ggcggcgcgg cggtccgatg agcggcccta
     8221 cctgcgcatt gccgatgaag ggcaaggtcc gcagcggccg gccggcgaag acccggcccg
     8281 gtctcacttc ggcatcgacg tgatgcgcga acggatggtc tcgatcggcg gcgcgctgga
     8341 ctggcaggct tctccccagg gcggggtcac ggtgctggcc tcgttcccga aggtggtgtg
     8401 atcatggcgg tgcgaatcct gatcgttgac gatcaccagc tcgcccgcga agggctgaag
     8461 gcggtcctgt ccgacgatgg cctggccgtg gtgggtgagg cggcgagcgg ggaggaggcg
     8521 gtcgccctgg tcagccagct ggtgcccgac gtggtgctga tggacgtgcg gctgggcccc
     8581 gggatcgacg ggctggaagc cacccgccgg atcgccgcgc tgggcctgcc cagccgggtg
     8641 atcatgctct cgctccacga catgccggtc tatgtccgcg aggcgctggc ggccggtgcg
     8701 gccggctatg tcctcaagga cgcttcgatt gccgagctgc gcggcgcggt cagccaggtc
     8761 atggccgggc agagcgtgat cccgctgggc ctgatcaacg ccgcgctgcg cgacagtgaa
     8821 cgcgaagtcc gcccggccga ggtcctcgcc gcgctgaccg ggcgcgagcg cgaagtgctc
     8881 gatctggtgg cggaaggcct gaccaacaag gaaatcgccc gccggctggc cgtcagcccg
     8941 gcgacggtca aggcccatgt cgagcggatc atcgccaagc tgggcgtgac cgaccgcacc
     9001 caggcggcgg tgctggtggc gcgggccgat ccggcgtgaa ccagccggtc ctctcccgca
     9061 agatgacccg cttctgggcc gaccggcgcc tggcgctgaa gggcctggtg gtgatcgccc
     9121 tgccgctggc gatcctgctg ggcgcgctgg tttcgctcta tatcgccagc aacgcggagg
     9181 cccgggccga gaacgatgtg cgccgcgcct ttgcgatcca gcgcgatacc taccaggtcc
     9241 acgccctgct ggccgaggcc gcggccggag tgcgcggcta tgccctgacc gggcaggaac
     9301 ggttcctgga gccctaccgc aaggccgagg ccgatctgcc gctgaccatg cagcggctgg
     9361 acgatgcgat ccgcgacggc gaggtgcgcc agcacttcaa ccggatcaac gacctgaccg
     9421 cccgcaagcg cgagggcctc aagggcattg tcgaactggc gcggcagggc gatcgcgccg
     9481 cgatcgagcg ggccctggtc gccaacaaga tcgtgctcga tgcgatgcgg cgcgagatcg
     9541 acctgatcca gcgccgcgag ggcgtgctgc tggaccagcg ccgtgcccgg gtggacgagg
     9601 tccgcgaccg cttcctgctg ctgaccgcga tcagcgccgt gatcggcctc ctcggcagcc
     9661 tggcggcggt ctatctgttc tccaccggga tcgttcgccg ggtccagatg ctggaggcca
     9721 acgccgaact gctggcccgc ggcgaggcgc tggccgaaat gcccgacgat gcggacgaga
     9781 tcggccggct ggcccagcgg atggcccgcg cgagcgcgct gctgcgcggc cgcgagcagg
     9841 ccctgcgcga aagcgaggag cggttccgtc tggtgatcga ggaggtccgc gattacggaa
     9901 tcttcgcgct ggatccccag ggggtcgtga cctcgtggaa tctcggggcc gagcgcatca
     9961 aggggtggga ggccgaggag atcctgggcc agcacttcag ccgcttctat cccgaagaga
    10021 cccgcggcca cctgccggcc gagatgcttg accgggcccg ccgcgacggc accgccgagg
    10081 acgagggctg gcgcctgcgc aaggacggca gcccgttctg ggccaacgtg gtgatcaccg
    10141 cgctccacga cgagcacggc gcgctacgcg gctttgccaa ggtgacccgc gacatgaccg
    10201 agcgccgccg ctcggaagag gcgctgcgct ttgccaacga ggaggcgatc gcggccaacc
    10261 tggccaagag cgaattcctc tcgcgcacca gccacgaact gcgcacgccg atgagcgcga
    10321 ttctgggctt cgggcaattg ctggagctgg acgaggacca gttcgagccg cgccaccgcg
    10381 aggcgctgag ccagatcatg aaggccgggc ggcacctgct ctcgctgatc aacgacctgc
    10441 tcgacatttc cagcatcgag gccggcgggg ccgaactggt gctcgagccg gtcgacctgg
    10501 gcgaggtgct gcaggaggtc cacggtctgg ccgcgccgat cgtggcagcc gccggcctgc
    10561 gctttgagct ggagccgccg gccgagccgc tggtcgcctg ggccgaccgc cgccgggtgg
    10621 tccaggtcat gctcaacctg atctccaacg ccgccaagta caaccgcacc ggcactctcg
    10681 tgcgcattgc ctgcctgccc cggggccgcg acgtgcgggt cgaggtggag gacgacggcg
    10741 aaggcgtggt cagcagcgaa gcctcgcgcc tgttcacgcc gtttgaccgg ctgggccagc
    10801 agaaccgggc caaagtggac ggcaccgggc tgggcctcgc cctgtccaag cggctgatcg
    10861 aatcgatggg cgggcagatc ggccacgagg ccccgccgca gggcgcgcgg ttctggttca
    10921 cccttccgac
//
LOCUS       DASUDV010000211         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_271759,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000211 DASUDV010000000
VERSION     DASUDV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271759"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..554)
                     /locus_tag="VFV30_04515"
     CDS             complement(246..554)
                     /locus_tag="VFV30_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847382.1"
                     /translation="MDCPLLECMGVIGGAWTPNILWHLRGGPRRFSELKIDIPPISAK
                     MLSTRLRELEELGVLRRKVMPTSPPSVEYSLDELGHRLIPAIEAIAKVGEELKQRKQG
                     "
     gene            737..1351
                     /locus_tag="VFV30_04520"
     CDS             737..1351
                     /locus_tag="VFV30_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4847383.1"
                     /translation="MKLYYMPGACSLAAHIVLNEIDADFELVAVDPASKQTALGEDFR
                     TISPNGYVPALSLDDGFALTEAPAILQFLADANPAAGLAPDNATRERAVLQQHLNFLA
                     SELHKAFAPFFSNPDLAGAEREAAIQRLSGRLDHLEALLGDGRDYLLGERFSVADAYA
                     GVIASWANHIGIGLGAWPSVGAFVARVVARPAAQDALRSEGLLN"
     gene            1426..1812
                     /locus_tag="VFV30_04525"
     CDS             1426..1812
                     /locus_tag="VFV30_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4847384.1"
                     /translation="MSEPIFAQIAARLRLYFDGLYYGDSKRLAEIFHPEARYVNTTDP
                     EHPVLAMPEYFEWVDRRDKPVDRNEPRDDAIVAISLAGPRAAVATVTCTMGARAYTDQ
                     LSLVCLDGTWRIMAKVFHYIPVPQPA"
     gene            1823..2032
                     /locus_tag="VFV30_04530"
     CDS             1823..2032
                     /locus_tag="VFV30_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX4847385.1"
                     /translation="MPYVNIKVTREGVSPEQKARLIAGTTQLLADVLGKDPATTFVVI
                     DEIALDSWGIGGLPVPEYRARRAAG"
BASE COUNT          398 a          731 c          679 g          393 t
ORIGIN      
        1 tgagcgtgaa ttcctggcgt tgcggggcct atcccgcgaa cagcccgatt gaatccaaac
       61 gccttttccc gagttagttg ataaagtgca tccgcgcagc gtcgtgtgcg cggcgaatgc
      121 tgccatgcca aaacggttgg ccgggttggg tgccgtcaat gtcaaaaggg ccgaaggctg
      181 ccctgcgcga ttcgcgccgg ggccgcgcgc gagcgtgaca gcaagccggc ctgtaggaca
      241 ggcgcctaac cctgcttgcg ctgcttgagc tcctcgccaa ccttggcgat cgcctcgatc
      301 gccgggatca ggcggtgccc gagttcatcc aggctgtatt cgaccgaagg cggcgaggtt
      361 ggcatgacct tcctgcgcag cacgccaagc tcttccagtt cgcgcagccg ggtcgacagc
      421 atcttggccg agatcggcgg aatatcgatc ttcagctcgc tgaagcggcg tggcccgccg
      481 cgcaggtgcc acaggatatt gggggtccag gcgccgccga tcacccccat gcattcgagc
      541 agcggacaat ccaccggtac cggcggactt tgattcttcc tgactttcaa caccatggat
      601 gaatggttcc gatgagctgg ttacttctgg gtaaccggta aacaaagtat accaagggag
      661 aaatatttac aaggtgttga tgcaacacta gctccagggt cagccagcgc gggccaagac
      721 actttggaga aatcacatga aactctacta catgcccggc gcgtgctcgc tcgccgcgca
      781 tatcgtcctc aacgaaattg acgcggattt cgaactggtc gcggtggacc cggccagcaa
      841 gcagaccgcg ctgggcgagg atttccgcac gatcagcccc aacggctatg tcccggccct
      901 gagcctcgac gatggctttg ccctgaccga ggcgccggcg atcctgcaat tcctggccga
      961 tgctaatccg gccgccgggc tggccccgga caatgccacc cgcgagcgcg cggtgctgca
     1021 gcagcacctc aacttcctcg cttcggaact gcacaaggcc tttgccccgt tcttcagcaa
     1081 tccggacctg gccggggccg agcgcgaagc cgcgatccag cggctgtcgg gccggctcga
     1141 ccatctcgag gcgctgctgg gcgatggccg ggactatctg ctgggtgaac gcttcagcgt
     1201 cgccgatgcc tacgccggag tgatcgcctc gtgggccaat cacatcggga tcgggcttgg
     1261 tgcctggccg tcagtcggcg ccttcgtggc acgcgtggtc gcgcgtcctg ccgcacagga
     1321 tgcattgcgc agcgaaggcc tgctgaactg actggctgca tcgccgcggg atttgtgtgc
     1381 cccccatccc gcggcgatgc ttgcccccga tccaaggaac ctggcatgag cgaaccgata
     1441 tttgcccaga tcgccgcgcg gctgcggctc tatttcgatg gcctctatta cggcgacagc
     1501 aagcggctgg ccgaaatctt tcatcccgag gcgcgctacg tcaacaccac cgatcccgaa
     1561 cacccggtgc tggccatgcc ggaatatttc gaatgggtcg accgccgcga caagcccgtc
     1621 gatcgcaacg aaccgcgcga tgatgcgatc gtcgcgatca gcctggccgg cccgcgcgcg
     1681 gcggtggcga ccgtaacctg caccatgggc gcccgggcct ataccgatca gctgtcgctg
     1741 gtctgtctcg atggaacctg gcggatcatg gccaaggtct ttcactatat ccccgtgccg
     1801 caacccgcct gaggaacccg tcatgcccta cgtcaatatc aaggttaccc gcgagggcgt
     1861 cagccccgaa cagaaggccc ggctgatcgc cggcaccacg cagctcctgg ccgatgtcct
     1921 cggcaaggat ccggccacca cctttgtcgt gatcgacgag atcgcactcg acagctgggg
     1981 gatcggcggg ctgccggtgc ccgaataccg cgcgcgccgg gccgcaggct agccgcccgc
     2041 gcgcctgccc agccagcgat cgagccagac cagcgccagc ccggtggcaa caaagcctgc
     2101 ggcaattccc gccatgttgg cgatcgccat ggtccagccc agcttgtcgg cgtccatgaa
     2161 gaaataggga tagcgcaggg tgctgcctgc gtcctgcagt t
//
LOCUS       DASUDV010000212         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_757785,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000212 DASUDV010000000
VERSION     DASUDV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757785"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1099)
                     /locus_tag="VFV30_04535"
     CDS             complement(179..1099)
                     /locus_tag="VFV30_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4847386.1"
                     /translation="MNEAWKVLTLAPRAVVEAALLAHEEALDWDPEIVISGSEEAEDR
                     PDDWQFEAWLPRRPSKADTDALRALFPDGYPEFFSVEQLPETDWLTASQQGLEPIRAG
                     RFYVHTPEHPPLNQPGVRDFAIPASQAFGTGQHATTAGCLEMLGEMKRRGLVVRNCAD
                     IGTGTGLLAFAALHLWPRALATASDIDAVCLGVVEDNARANGIRMGSRPGELTMLVAD
                     GMEHPLLKERGPYDLILANILAGPLIGLAPHFAKALVPGGHLLLAGLLETQEAQVRRA
                     ARAAGLRIAERKVNGDWSILWLRKRGRGQW"
     gene            complement(1096..1989)
                     /gene="bla"
                     /locus_tag="VFV30_04540"
     CDS             complement(1096..1989)
                     /gene="bla"
                     /locus_tag="VFV30_04540"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEX4847387.1"
                     /translation="MFGKFAVSALALLSLGATTAPADRPGASKDIAGACGTRDGWSDP
                     APPAKIHGKTWYVGTCGITVVLVETSAGLVLIDTGPEDAAPHVLNNVRALGFDPRQIK
                     WLLMTHEHFDHVGGMALIQKATGAKLVVSPGAERAMRSGKADPDDPQAALLEKYPMAP
                     TRVDRVLRSGGSLTIGGTRFTMHANPTHSPGSASWTWQSCEAGKCLTIAYADSVNTIS
                     SDDYHFTKHPERVEAARAGLRVIETLPCDLLLTPHPGGSALLERLSGKAPLVYPRACV
                     AYSAAGSDRLANRLAREAQGQ"
     gene            complement(1979..2698)
                     /locus_tag="VFV30_04545"
     CDS             complement(1979..2698)
                     /locus_tag="VFV30_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847388.1"
                     /translation="MTQLTALVTGSSRGIGAAIVQGLRARGVAVIGHATRAVDASTIP
                     ADLTDPTAPSALWDEAIDRSAHGRIDLLVNNAGLFQANPIGLSDIEWLDNWEDTLRIN
                     LTAAAELSRLAVKHWLAGDRAGRIVHIASRAAYRGDSPDHWHYAAAKGGMIAMHKTIA
                     RQYAARGVMSYAICPGFTDTAMAGDYLASRGGPGLLADIPLGRVAAPEEIAAMAVFCA
                     LDAPESMTGAVLDANGASYVR"
     gene            2849..>3103
                     /gene="sdhC"
                     /locus_tag="VFV30_04550"
     CDS             2849..>3103
                     /gene="sdhC"
                     /locus_tag="VFV30_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX4847389.1"
                     /translation="MATKNRPISPHLQVWRWGPAMLVSILHRVTGDGMALVGLGVLIY
                     WLASLAGGASAYADFTAHASAWYGKVVLVGLTWAFFTHMAS"
BASE COUNT          453 a         1051 c         1087 g          512 t
ORIGIN      
        1 gtgttggccg gcgtgagcat gccgccgact ctttccagtc gaacgttctc gaacccgcag
       61 gcttcggccg cgacggcgat ccgctccatc gatggttcgt cggaaatgta agagctcatc
      121 agttcgcgtg gcccagcaca agcggcgacg gcgaggacga gcacgatcgc gccgcggctt
      181 accactggcc acgaccgcgc ttgcgcagcc acaggatcga ccagtcgccg ttgaccttgc
      241 gttcggcgat ccgcagcccg gcggcgcggg ccgcgcggcg gacctgtgct tcctgcgttt
      301 ccagcaggcc ggccagcagc aggtgcccac ccgggaccag cgccttggcg aagtgcggcg
      361 ccaggccgat cagcggcccg gccaggatat tggccaggat caggtcatag ggcccgcgct
      421 ctttcagcaa cggatgctcc atcccgtcgg cgaccagcat ggtcagctcg cctggacggg
      481 agcccatccg gatgccgttg gcgcgggcgt tgtcctccac cacgccaagg cagaccgcgt
      541 cgatgtcgct cgccgtggcc agcgcgcggg gccacaggtg cagcgcggca aaggccagca
      601 ggccggtgcc ggtcccgata tcggcgcagt tgcgcaccac cagcccgcgc cgcttcattt
      661 cgcccagcat ttccaggcac ccggcggtgg tggcgtgctg gccggtgccg aaggcctggc
      721 tggcgggaat tgcgaaatcg cgcacgccgg gctggttcag cgggggatgc tccggcgtgt
      781 ggacataaaa gcgcccggcg cggatcggct ccagtccctg ctggctggcg gtcagccagt
      841 cggtctcggg cagctgctcg accgagaaga actccgggta gccgtccggg aacagcgcgc
      901 gcagcgcatc cgtgtcggct ttgctcggcc ggcggggcag ccaggcttcg aactgccagt
      961 cgtccgggcg gtcctcggcc tcctcgctgc cggaaatgac gatctccgga tcccagtcga
     1021 gcgcttcctc gtgcgccagc agcgctgctt caaccacggc gcgcggggca agggtcagga
     1081 ctttccaggc ttcgttcact ggccctgggc ctcccgcgcg aggcgattgg ccaaccggtc
     1141 gctgcccgcg gcggaatagg ccacgcaggc gcgcggatag accagcggcg ccttgcccga
     1201 tagccgttcg agcagggcgc tgccgccggg gtgaggggtg agcagcagat cgcagggcag
     1261 cgtttcgatt acccgcagcc cggcccgcgc cgcctcgacc cgctcggggt gcttggtgaa
     1321 gtggtaatcg tcggacgaga tggtgttgac gctgtcggca taggcgatgg tcaggcattt
     1381 acccgcctcg cagctttgcc aggtccagct ggccgacccg ggggagtggg tcgggttggc
     1441 gtgcatggtg aagcgggttc ccccgatcgt cagcgagccg ccgctgcgca gcacgcggtc
     1501 aacccgggtc ggcgccatcg ggtacttttc cagcagcgcg gcctgcgggt cgtccggatc
     1561 ggccttgccg ctgcgcatcg cccgctcggc accggggctg accaccagct ttgcgccggt
     1621 tgccttctgg atgagggcca tccctccgac atggtcgaaa tgctcgtgcg tcatcagcag
     1681 ccatttgatc tggcggggat cgaagccgag cgcgcggaca ttgttgagca catgaggggc
     1741 ggcgtcttct ggccccgtgt cgatcaggac cagcccagcc gacgtttcga ccagcaccac
     1801 ggtgatcccg caggtgccga cataccatgt cttgccatgg atcttcgcgg gcggcgctgg
     1861 atccgaccag ccgtcgcggg tgccgcatgc cccggcgatg tctttgctgg cgccgggccg
     1921 gtccgccggt gcggtcgtgg cccccaggct cagcaaggcg agggcggaaa cggcgaactt
     1981 accgaacata gcttgctcca ttggcatcca gcaccgcccc ggtcatgctt tccggcgcgt
     2041 cgagggcaca gaacaccgcc atggccgcga tctcttcggg cgcggcaacc cggccgagcg
     2101 ggatgtcggc caagagcccc ggtccgccgc ggctggcgag gtagtccccg gccatcgccg
     2161 tatcggtaaa gcccgggcag atcgcatagc tcatcacgcc ccgggcggcg tattgccggg
     2221 cgatggtctt gtgcatggcg atcatcccgc ccttggccgc ggcatagtgc cagtgatcgg
     2281 gcgaatcccc gcggtaggcg gcgcggctgg cgatgtggac gatccggccg gcccgatcgc
     2341 ccgccagcca gtgcttcacc gccaggcgcg acagctcggc cgccgcggtc aggttgatcc
     2401 gcagcgtgtc ctcccagttg tcgagccatt cgatgtcgct gaggccgatc gggttggcct
     2461 ggaacagccc ggcgttgttg accagcaggt cgatccggcc gtgggccgaa cggtcgatcg
     2521 cttcgtccca cagcgcggag ggggcggtgg gatcggtcag gtcggcggga atcgtgctgg
     2581 catcgaccgc gcgggtggca tggccgatca cggcaacccc gcgggcgcgc agcccctgga
     2641 caatggccgc gccgatcccg cgcgaggaac cggtcacaag tgcagtcagc tgggtcatgc
     2701 ccccgctcta ggcaggtttg ccgcgaatgg gaatcgcgat tgctggaaat acgaccttca
     2761 gtgcgcttgc gcgcctcggc gttttgatta aggggagttc ggaatcgggg ccgcgcgccc
     2821 cggccaagca cagagggaaa cggcgcaaat ggccaccaag aaccgcccga tttcaccgca
     2881 cctgcaggtg tggcgctggg gaccggcgat gctggtttcg atccttcacc gcgtgaccgg
     2941 agacggcatg gccctggtcg gcctgggcgt gctgatctac tggctggcct cgctggccgg
     3001 cggcgcttcg gcctatgccg atttcaccgc ccacgcctcg gcctggtacg gcaaggtcgt
     3061 gctggtcggc ctgacctggg ccttcttcac ccacatggct tcg
//
LOCUS       DASUDV010000213         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_823933,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000213 DASUDV010000000
VERSION     DASUDV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823933"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VFV30_04555"
     CDS             complement(<1..1587)
                     /locus_tag="VFV30_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847390.1"
                     /translation="MAKQKRKAPASKPVTQHQLFPAVVALWFGALFGLGSLAVRPGLV
                     EDLVIKSRIDLIVPAAAPPLGVTARMLIALCLAALGAMVGAVIGRRLARPKAEARERK
                     RSNLATPAPAPTYRQDDALEVSATPANRRRALAIEEEEAKFVPLDLAPLPGAAPQVLD
                     IAAIKLDMNPAENAAAPQPAPFVAPPPALPAAPNLDWNSAAPAAAPVHVAAQPMPIPA
                     AMARQVDPGAFAAPQAQPAAEIASPFASPAAPPLADDPATSAQTAAQAGRQVFGLAPE
                     AQPDPNPARQIFGAPVIEDHVPKEFVEAHGFKASVFDVPEPTPLFVPRTAPDQPIAAP
                     VPAPSLAEPAVKPAAMPIETVIQPVAESAPAPQPAEPLPSPAGLGMEDLSARLAESMR
                     RRRAARDQQGAATPAQAPAAAPAPAPSEPVVEASIAAPGPFGFEPVQQPLAGTFAAPA
                     PEPQAFEPALAPAPAPEPAPFAMPAAFEAAPPAPLAEAAAAPVAMPAAMRPLNLGGFE
                     EAEAPLESLLPPRLAFTTEVV"
     gene            complement(1637..>2203)
                     /locus_tag="VFV30_04560"
     CDS             complement(1637..>2203)
                     /locus_tag="VFV30_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4847391.1"
                     /translation="SFYDYEGLDDLIAAMPLLLARHGDARLLLVGGGPMEAALKAQAA
                     ASPAADAIHFVGRVPHSEVERYYALVDVMAYPRKKSRLTDLVTPLKPLEAMAQNQLVA
                     ASDVGGHRELIRDGQTGTLFAPDDPAACAAALADLLEARDQWEARRRAGREHVARNHD
                     WQANIYRYQDVYQIPLGRSVDCSVPRAA"
BASE COUNT          322 a          702 c          846 g          333 t
ORIGIN      
        1 cacgacctcg gtcgtaaacg ccaggcgcgg gggcagaagg ctttcgagcg gagcctcggc
       61 ctcctcaaag ccgccaagat tgagcggtcg cattgccgcc ggcatggcga ccggagcggc
      121 cgcggcctcg gcgagcggag ccggcggggc agcctcgaac gcggcgggca tggcgaaggg
      181 tgccggttct ggcgcagggg ccggggccag cgcgggttcg aacgcttgcg gttcgggcgc
      241 gggcgcggcg aatgtccctg ccagcggctg ttggaccggc tcgaacccga agggccctgg
      301 cgctgcaatg gaagcttcga ccacgggctc ggaaggtgcg ggtgcgggtg cggcggcggg
      361 ggcttgggcg ggtgtggcag cgccctgttg atcgcgcgcg gcgcggcggc ggcgcatcga
      421 ttcggcaagg cgcgcggaaa ggtcttccat gccaaggcct gccgggctcg gcagcggctc
      481 ggccggctgc ggtgcgggcg cggattcggc aaccggttgg atcacggtct cgatcggcat
      541 ggcggctggc ttgacggccg gttcggcaag gctgggcgcc gggaccggtg cggcgatggg
      601 ctggtccggc gcggtccgcg gaacgaacag cggggtcggt tcgggaacgt cgaacacgct
      661 ggccttgaag ccatgggctt cgacgaattc cttgggcacg tggtcttcga tcaccggggc
      721 gccgaagatc tggcgggcgg ggttgggatc gggctgggcc tcgggggcaa ggccaaaaac
      781 ctggcggcca gcctgcgcgg cggtctgggc agaggtcgcc ggatcgtcgg ccaacggcgg
      841 cgcggcagga ctggcgaagg gcgaggcgat ctcggcagca ggctgggcct ggggcgcggc
      901 gaaggcgccg ggatcgactt gcctggccat ggcggccgga atgggcatcg gctgcgcagc
      961 gacatggacc ggcgccgcgg caggtgcggc actgttccaa tcgaggtttg gcgcggccgg
     1021 cagcgctggc ggcggagcga caaagggtgc tggttgcggg gcggcggcat tttccgctgg
     1081 gttcatgtca agcttgatcg ccgcgatgtc gagcacctgc ggcgcagcgc cgggaagcgg
     1141 ggcaagatcg agcgggacga acttcgcctc ctcctcctcg atcgccagcg cacggcggcg
     1201 attggcggga gtggccgaaa cctcgagcgc atcgtcttgc cgataggtcg gcgccggggc
     1261 cggggtggcc aggttggagc gcttgcgttc gcgcgcctcc gctttcggtc gggccaggcg
     1321 gcggccgata accgcgccga ccatcgcgcc cagcgcggca aggcacagcg cgatcagcat
     1381 ccgcgcggta acccccagcg gcggggcggc ggcgggaacg atcagatcga tccggctctt
     1441 gatcaccaga tcttcgacca ggccggggcg gacggcaagg ctgcccagtc cgaacagggc
     1501 tccgaaccac aatgccacaa cggccggaaa cagctgatgc tgcgtcaccg gtttgctggc
     1561 tggcgccttg cgtttctgct tggccacgtt ttttgcttcc ctgttcaaac ccttgcccgg
     1621 gatctgccgt gcgccttcag gcagcccggg gaaccgaaca atccactgat ctgcctaagg
     1681 gaatttggta aacatcctga taacgataaa tattcgcctg ccagtcgtgg ttcctggcaa
     1741 catgctcgcg gccggcccgc cgccgggctt cccactgatc gcgcgcctcg agcagatcgg
     1801 ccagcgccgc ggcgcaggcc gccggatcgt cgggcgcgaa cagcgtgccg gtttgcccgt
     1861 cgcggatcag ttcgcggtgc ccgcccacgt cggaagcggc gaccagctgg ttctgggcca
     1921 ttgcctcgag cggcttgagc ggggtgacga ggtcggtcag gcggcttttc ttgcgtggat
     1981 aggccatgac atcgaccagc gcgtaatagc gttcgacctc gctgtgcggg acgcggccga
     2041 cgaagtggat cgcatccgcc gccgggctgg ccgcggcctg cgccttgagc gcggcctcca
     2101 tcggcccgcc gcccaccagg agcagccgcg catcgccatg gcgcgccagc agcagcggca
     2161 tcgccgcgat cagatcgtcg agcccctcat agtcgtagaa cga
//
LOCUS       DASUDV010000214         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_514988,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000214 DASUDV010000000
VERSION     DASUDV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514988"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..>2145
                     /locus_tag="VFV30_04565"
     CDS             104..>2145
                     /locus_tag="VFV30_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847392.1"
                     /translation="MVAIILVLAALGKNLPFEPYDWLQKSVTARLNAKPYTGDAIIIE
                     IDSQTFEGLGRPALDDRDLARLIRNLDTAKARRIVLGRIRQRSASQHSSPELLEALDA
                     ASPKVVLFTELVPKKSSAFSWATFRPANGQAYFHDPGVDPPTAQRVQLASEISSTTPF
                     GAPWALPPTVRLGDRNYPSIAQILAGQPQPIFEKYDVALQIDPGSIPTLNAIDVISGR
                     FDPAALRGKQALIYSSSDVTRDAIVTPFGRLPSAAPITVMAAETLRNGPPLMLGWIPS
                     FLVAVLGVIGWLTLRTPLLRRGAFALAFVCVVASPLVLERLLIFQTTSNAVFLMILIA
                     LGRQWSKFRATLALARSAAETKSWFLAQASHDLRQPIHAIGMLAARLGQTDLNPAQAD
                     LARKIDRSIEAANRMLQSLLDLATIESGSLSPQPAPISVNALLAELDEQAGPLAERAG
                     VELRFVPCEATIMADRTLTLAMLQNVVANSIKYATGKRVLVGARRTGKTIALCVHDVG
                     QGISDSDMRHVRKAFFRASSHRKSSLDGAGLGLAIVHQLASLMNLTFTLRSAPGRGTS
                     AIIAGFARARLDPAPTGLSESKAIRPLAGLRVLLVDDDEASLAATAALLEQWGCIVQA
                     QDHFPANVTKTDIIVSDFDFGKGHTLADHIGQVEACAGAGIPTLIVSGHQPDAVRTS"
BASE COUNT          355 a          755 c          641 g          394 t
ORIGIN      
        1 ttcgagttgg ggacacagga tgacaggacg ccgcatcggg gcggtttggg cacagctgag
       61 aggccttggc gcccgcctct ggcgggatcg cacggcccgc tatgtggtcg cgatcatcct
      121 ggtcctggcc gcgcttggca agaacctgcc gttcgaacct tacgactggc tgcaaaagtc
      181 ggtgaccgcc cggctcaacg ccaagcccta caccggcgat gcgatcatca tcgagatcga
      241 cagccagaca ttcgaagggc ttggccgccc cgctcttgac gatagggatc ttgccaggct
      301 gatccgcaat ctcgacacgg ccaaggccag gcggatcgtg ctgggccgga ttcgccagcg
      361 ttcagcctcg caacactcca gccccgaact gctcgaagcg ctggatgcgg ccagccctaa
      421 ggtggtcctg ttcaccgagc ttgtccccaa gaagtcgagc gccttttcct gggccacatt
      481 caggcccgcc aatggccagg cctatttcca cgatccgggc gtcgatccgc cgaccgcgca
      541 gcgggtccag ctggcaagcg aaatcagttc caccacgccc ttcggtgcgc catgggccct
      601 gcccccgaca gtgcggctcg gcgatcggaa ctacccttcg atcgcccaga ttctggccgg
      661 ccagccgcag ccgatcttcg aaaagtacga cgtcgccctg cagatcgatc cgggttcgat
      721 ccccacgctc aatgcgatcg acgtgatctc gggccggttc gatccggccg cactccgcgg
      781 caagcaggcg ctgatctatt catcgtctga tgtcacccgc gatgccatcg taacaccatt
      841 cggccgcctg ccttcggccg cgccgatcac cgtcatggca gccgaaacgc tgcgcaacgg
      901 cccgcccctg atgctgggtt ggattccatc gttccttgtc gcggtgctgg gcgtgatcgg
      961 ctggctgact ttgcgcactc cgttgctgcg gcgcggcgct ttcgccctcg cgttcgtctg
     1021 cgttgtcgca tcgccgctgg tccttgaacg gctgttgatc ttccagacaa cgtccaatgc
     1081 ggtgttcctg atgatcctga ttgcgctggg ccgacaatgg agcaagttca gggcaaccct
     1141 ggctctggcc cgttcggccg ccgaaaccaa gtcgtggttc ctggcccagg ccagccacga
     1201 tctgcgccag ccgatccatg cgatcggcat gctcgctgcg cgccttggcc aaaccgatct
     1261 caatccggcc caggccgacc ttgcccgcaa gatcgaccgc tcgatcgagg cggcgaaccg
     1321 catgctgcaa tcactgcttg atctggcgac gatcgaaagc ggttcgctat cgccgcagcc
     1381 tgccccgatt tccgtcaacg ccttgctcgc cgagctcgac gagcaggccg ggccgctggc
     1441 cgaacgcgcc ggtgtggaac tgcgcttcgt cccctgcgag gccacgatca tggccgaccg
     1501 cacgctgacg cttgcgatgt tgcagaacgt cgtcgccaat tcgatcaagt acgcaaccgg
     1561 caagcgcgtt ctggtcggcg cgcggcggac cggcaagacc attgcgcttt gcgtccacga
     1621 cgtcggccag ggcatatcag actccgatat gcggcatgtg cgcaaggcct tcttccgcgc
     1681 atcctcgcac cgcaagagca gccttgacgg ggccgggctt ggcctggcga tcgtccacca
     1741 gctggccagc ctgatgaacc tcaccttcac gctgcgctcc gccccgggcc ggggcacctc
     1801 ggccatcatt gccggtttcg cccgggcccg gctcgatccc gcgcccaccg gactctccga
     1861 atccaaggcg atccgtccgc tggcgggcct gcgggttctg ctggtcgatg atgacgaagc
     1921 ctcgctcgcg gccacggccg cactgctcga acaatggggc tgcattgtgc aggcacagga
     1981 ccattttccg gccaatgtca ccaagaccga catcatcgtc agcgacttcg atttcggcaa
     2041 gggccacacc ctcgccgacc atatcggcca ggtcgaagcc tgcgcgggtg cgggcattcc
     2101 cacgctcatc gtctcgggcc atcagcccga tgcggtgaga acgtc
//
LOCUS       DASUDV010000215         6262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1244027,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000215 DASUDV010000000
VERSION     DASUDV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244027"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFV30_04570"
     CDS             <1..904
                     /locus_tag="VFV30_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX4847393.1"
                     /translation="DDLLAEKEELEKLLDSPARQRTRLKRDLATLRKDYAEDTALGRR
                     RTTVAEAAPTVEFSMDAMIEKAPVTVILSAKGWIRGASGHEALDKEFKFKEGDGPAFA
                     LHAQTTDKLLVACDNGRFYTLGCDKLPSARGFGEPIRTMVDIDAGAEIVALLVYKPKA
                     QLLLASSTGRGFAAEADELLAETRKGRGVMTTKPGVKLAVVREIPPEHDHVAVVGDNR
                     KLVLFSLEELPVLAKGQGVTLQRYRDGGLADATTLKLEDGLSWKMGGDTGRTRTESDM
                     RLWKVARGAAGRMPPTGFPRDNKF"
     gene            complement(901..1524)
                     /locus_tag="VFV30_04575"
     CDS             complement(901..1524)
                     /locus_tag="VFV30_04575"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX4847394.1"
                     /translation="MTGVGELAMAQPLLILAILAILAASAGWMIEHERPAFGLALRRS
                     GYLGMLAAGLLLVGQTAHDASKSDANLILGSRPGLTVEGQTTVIPMASDGHFWITARI
                     NGQDTELMIDTGATYTSLSHGAAKAAGLVPDNAKPPALLNTANGVIEARFSRIEELRF
                     GTITARQLEAIVLPAASGDTNVVGMNLMNQLGSWRVENDRLILEPKR"
     gene            complement(1521..2690)
                     /locus_tag="VFV30_04580"
     CDS             complement(1521..2690)
                     /locus_tag="VFV30_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX4847395.1"
                     /translation="MTRLPDAAWRTRAGLGVLIQALGPGQARYVGGAVRDTLLGLAVK
                     DVDLATPLDPHEVMRRLKAHSIQVIPTGIEHGTVTAVLKDGPVEITTLRRDVSTDGRR
                     ATVAFAQDWREDAARRDFTINALYADPASGEIFDWFGGLDDLAARRVRFIGDARQRIR
                     EDHLRILRYFRFQARFGSQPADAEAESAVSELAATLKGLSRERVGMEMMNLLGLPDPA
                     PTVARMAALGVLDVVLPEADVAALTALTAEEAKQGAAPDALRRLAALLPPDGPLAEAV
                     ASRFRLSGAQKKRLALAAGRGDGPADPRALAYRLGRDAALDRLLLTGGSVAPLSGWDI
                     PTFPLKGGDVVARGVKAGPEVARTLRAVEAGWIAAGFDDAAVPALLDAELAGGAA"
     gene            complement(2687..3268)
                     /locus_tag="VFV30_04585"
     CDS             complement(2687..3268)
                     /locus_tag="VFV30_04585"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX4847396.1"
                     /translation="MSLKQRLARLHAEGHAAGIDHRDEVDFAADTLRPAAVLAAVTER
                     ADRPGFLLIHRPSNMRSHPGQVAFPGGKIDPGESAEEAALREAWEELGIHERDVHVIG
                     PSDTYRTGSGYAITPVLAVVPADLKLNPSPTEVAAWFEAPVDFVFDPANHTHHSGFWQ
                     GRTRSYIEILWQEHRIWGVTAGIIANLAKRIAW"
     gene            complement(3265..3804)
                     /locus_tag="VFV30_04590"
     CDS             complement(3265..3804)
                     /locus_tag="VFV30_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX4847397.1"
                     /translation="MPYVPPPDLATLSLAEIAELSAARKLPPVEQWNPESRSDSRMVI
                     KADGRWFHDGGEIRRPAMIRAFSGLLRRDPDGQHWLVTPFEMQAIAVEDAAFIATDVT
                     RTGDALAFRLNTDEVVVAGPDNPLIARGDPDLPALYLAVRHGTEARINRSTWLQLVDL
                     AGEDLTVTSQGAVFPLVPR"
     gene            complement(3828..4346)
                     /locus_tag="VFV30_04595"
     CDS             complement(3828..4346)
                     /locus_tag="VFV30_04595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4847398.1"
                     /translation="MADVATLIPLDNVDPALVEALLDRAFEPERHKRTAYKVREGTDW
                     LPGLSFAAIDENDHLVGTIQCWPVALTDPQGRAHPMIMVGPVAVLPEQQGQGFGKALM
                     TASLAALSPQAPLPQVMIGDPEYYGRFWGFTNEATGGWTLPGPFEQHRLLARCANPAV
                     LPKEGMLGPWRG"
     gene            complement(4401..5513)
                     /locus_tag="VFV30_04600"
     CDS             complement(4401..5513)
                     /locus_tag="VFV30_04600"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4847399.1"
                     /translation="MRNNLLMAFSPLVLIAASCGGAASGDSPPKIQKVEGHFATEELA
                     TFEEPWALAVLPDGGVLVTEKSGKLKLRKADKTVVEIALPFTVAYGGQGGLGDVIAAP
                     DFAKSGRIYLSYAEAGEGDTRGAAVGTGVLKMTGGTVTLDGFEVIWRQNPKVTGRGHY
                     SHRLAISPDRKALFIASGDRQKMQPAQDPASDLGKVIRLPIDASGKPAGAPVRFSLGH
                     RNILGMQFAPDGRLWEIEHGPAGGDELNLVKQGANYGWPVVSEGRHYGGEFIPHHDTR
                     PDFAAPALYWNPVIAPGGMIFYSGKMWPEWKGQLLIAALNPAGVVRVKIKGDKASEEA
                     RYPMAKRIRAIAEAADGSLLVLEDKAGGRLLRLTRK"
     gene            5512..6147
                     /locus_tag="VFV30_04605"
     CDS             5512..6147
                     /locus_tag="VFV30_04605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX4847400.1"
                     /translation="MVRNIGDGGRFAKCPEGQVVIGVDEAGRGPLAGPVVAAACLLGP
                     HAPAGLDDSKKLSAKRRAALEPQIQASCRWAVAVVEADEIDRINIFAATMLAMTRAVG
                     ALVAELGQEPDMVLIDGNKTPAGRVPEWRWTARAIIGGDALEPCISAASILAKEHRDR
                     VMRAHAEAHPHYGWERNMGYGTAEHLEALRVHGPSPIHRKSFAPVAQLVLL"
BASE COUNT          977 a         2149 c         2201 g          935 t
ORIGIN      
        1 ggacgacctg ctggccgaga aggaggagct ggaaaagctg ctcgacagcc cggcccgcca
       61 gcgcacccgg ctgaagcgcg atctggccac tctgcgcaag gactacgccg aggacaccgc
      121 gctcggccgc cgccgcacga ccgtggccga ggccgcgccg acggtcgaat tctccatgga
      181 cgccatgatc gagaaggcgc cggtcacggt gatcctctcg gccaagggct ggatccgcgg
      241 cgcgagcggg cacgaggcgc tcgacaagga attcaagttc aaggaaggcg acgggccggc
      301 cttcgcgctc cacgcccaga ccaccgacaa gctgctggtc gcttgtgaca acggccgctt
      361 ctacaccctg gggtgcgaca agctgccttc cgcgcgcggc tttggcgagc cgatccggac
      421 catggtcgat atcgatgcgg gcgcagagat cgtcgccctg ctggtctaca agcccaaggc
      481 ccagctgctg ctggccagca gcacaggccg cggctttgcg gccgaggcgg acgagctgct
      541 tgccgaaacc cgcaagggcc gcggggtgat gaccaccaag ccgggcgtga agctggccgt
      601 tgtccgcgaa atcccgcccg agcacgatca cgtggccgtg gtgggcgaca accgcaagct
      661 ggtgctgttc agcctggaag agctgccggt gctggccaag ggccagggcg tcacgctcca
      721 gcgctaccgt gacggcgggc tggccgatgc gaccacgctg aagctggaag acgggctcag
      781 ctggaagatg ggcggcgaca ccggccggac ccggaccgaa agcgacatgc ggctgtggaa
      841 ggtcgcccgc ggcgccgccg ggcggatgcc gccgacgggt ttcccgcgcg acaacaagtt
      901 ctagcgcttc ggctccagga tcagccggtc gttctcgacc cgccagctgc ccagctggtt
      961 catcaggttc atgccgacca cgttggtatc gccgctggcc gcgggcagga cgatcgcctc
     1021 cagctggcgc gcggtgatcg tgccgaagcg cagctcctcg atccgcgaaa agcgggcctc
     1081 gatcactccg ttggcggtgt tgagcagggc cgggggcttg gcgttgtccg ggaccagccc
     1141 ggcggccttg gccgcgccgt gcgacaggct ggtataggtc gcgccggtgt cgatcatcag
     1201 ttcggtgtcc tgcccgttga tccgggcggt gatccagaaa tgcccgtcgc tggccatcgg
     1261 gatcacggtg gtctgcccct cgaccgtcag gccggggcgg gagccgagga tcaggttcgc
     1321 gtcggatttc gaggcatcgt gcgcggtctg gccgaccagc agcagtccgg cggcgagcat
     1381 cccgagatag cccgagcggc gcagcgccag gccgaaggcc gggcgctcgt gctcgatcat
     1441 ccagccggcc gatgcggcca ggattgccag gatcgccagg atcagcagcg gctgggccat
     1501 cgccagttcg ccaactccgg tcatgccgcg cctcctgcca gttcggcgtc aagcagcgcg
     1561 gggaccgcgg catcgtcgaa cccggcggcg atccagcccg cctcaaccgc gcgcagggtc
     1621 cgcgccacct ccggcccggc ctttaccccg cgcgcaacga catcgccgcc cttcagcggg
     1681 aaggtgggaa tgtcccagcc gctcagcggg gccacgctgc ccccggtcag cagcaagcgg
     1741 tcaagcgccg catcgcggcc caggcgatag gcgagggcgc ggggatccgc tggcccatcg
     1801 ccgcgcccgg cggccagggc gaggcgcttt ttctgcgcgc ccgaaagccg gaaccggctt
     1861 gccacggctt cggccagcgg cccgtcgggg ggcagcagcg ccgccagccg gcgcagcgca
     1921 tcgggagcgg cgccctgctt cgcttcctcg gccgtcagcg cggtgagcgc agccacatcg
     1981 gcttccggca ggaccacatc cagcacgccc aacgcggcca tccgcgccac ggtcggcgcg
     2041 ggatcgggca ggccgagcag gttcatcatt tccatgccga cccgttcgcg cgacaggccc
     2101 ttgagcgtgg cggccagttc agagacagca ctttccgctt cggcatcagc cggttgcgag
     2161 ccgaacctgg cctggaaccg gaagtagcgc aggatccgca aatggtcctc gcggatccgc
     2221 tggcgggcat cgccgatgaa ccgcacgcgc cgcgcggcca gatcgtccag cccgccgaac
     2281 cagtcgaaaa tctcgcccga ggcgggatcg gcatagagcg cgttgatggt gaaatcgcgc
     2341 cgcgcggcat cctcgcgcca atcctgcgca aaggccacgg tcgcgcggcg gccatcagtt
     2401 gaaacgtcgc ggcgcagggt cgtgatctcc accggcccgt ccttcagcac ggcggtgacc
     2461 gtgccgtgct cgatcccggt ggggatgacc tggatcgaat gggccttcag ccggcgcatc
     2521 acctcgtgcg ggtccagcgg ggtagcgagg tccacatcct tcaccgccag gcccagcagg
     2581 gtgtcgcgca ccgcaccgcc aacatagcgg gcctggccgg ggcccagcgc ctggatcagc
     2641 acgccaaggc ccgcgcgcgt gcgccaggcg gcatcgggca ggcgggtcac cacgcgatcc
     2701 gcttcgccag gttggcgatg atccccgcgg tcacgcccca gatccggtgt tcctgccata
     2761 ggatctcgat atagcttctt gtccggcctt gccagaaccc ggagtggtgg gtgtggttgg
     2821 ccggatcgaa cacgaagtcg accggggcct cgaaccaggc ggcgacctcg gtcgggctgg
     2881 ggttgagctt gaggtcggcc gggaccaccg ccagcaccgg cgtgatcgca tagccgctgc
     2941 cggtgcggta ggtgtcgctg gggccgatca cgtggacgtc gcgctcgtga atgccgagct
     3001 cttcccaggc ctcgcgcagc gcggcttcct cagcgctttc gcccggatcg atcttgcccc
     3061 ccggaaaggc gacctggccc gggtgcgaac gcatgttcga cgggcggtgg atcagcagga
     3121 acccgggccg gtccgcgcgc tcggtcaccg cggccagaac cgcggcgggg cgcagggtgt
     3181 cggcggcaaa gtccacctcg tcgcggtggt cgatgccggc ggcgtgaccc tcggcatgga
     3241 gccgggcaag gcgctgtttc aggctcaccg cggcaccagc gggaagacgg cgccctggct
     3301 ggtcacggtc aggtcctcac cagccagatc gaccagctgc agccaggtgc tgcggttgat
     3361 ccgggcctcg gtcccgtggc gaaccgccag ataaagcgcc ggcagatcgg ggtcaccgcg
     3421 cgcgatcagc ggattgtccg gcccggccac caccacttcg tcggtgttga ggcgaaaggc
     3481 cagggcatcg ccggtgcggg tcacgtcggt ggcgatgaag gcggcatcct ccaccgcgat
     3541 tgcctgcatc tcgaacgggg tcaccagcca gtgctggccg tccggatcgc gccgcaacag
     3601 gccggaaaag gcccggatca tcgccgggcg gcggatttcc ccgccatcgt ggaaccagcg
     3661 gccgtcggcc ttgatcacca tccggctgtc gctgcgggat tccgggttcc attgctcgac
     3721 cggcggcaat ttgcgcgcgg cgctcagctc cgcgatttcg gcaaggctca gcgtggcaag
     3781 atcaggaggc ggaacatagg gcatcggaac tgcgatgcca gatcggccta gccgcgccaa
     3841 ggccccagca tgccttcctt gggcaggact gccggattgg cgcagcgcgc cagcaggcgg
     3901 tgctgctcga acgggccggg cagggtccac ccgccggtcg cttcgttggt gaagccccag
     3961 aaccggccgt agtactccgg atcgccgatc atcacctgcg gcagcggggc ctgcgggctg
     4021 agcgcagcga ggctggcggt catcagcgcc ttgccaaagc cctggccctg ctgttccggc
     4081 agcacggcga ccgggccgac catgatcatc ggatgggcgc ggccctgcgg atcggtcagg
     4141 gcaaccggcc agcactggat cgtgccgacc aggtgatcgt tctcgtcgat cgcggcaaag
     4201 gacaggccgg gcagccagtc ggtcccttcg cgcaccttgt aggcggtgcg cttgtgccgc
     4261 tcgggctcga aggcgcggtc gagcagcgcc tcgaccagcg cgggatcgac gttgtcgagc
     4321 gggatcaggg ttgcgacatc ggccatggcg cgcgccgata gggtgcgcgc gccgtcctgt
     4381 ccaatcgaat ccggtggggc ttacttgcgg gtcagccgca gcaggcggcc gccggccttg
     4441 tcttccagca ccagcagcga accgtcggcg gcttcggcaa tggcacggat ccgcttggcc
     4501 atcggatagc gggcttcctc gctggccttg tcgcccttga tcttcacccg caccacaccg
     4561 gccgggttca gcgccgcgat cagcagctgg cccttccatt ccggccacat cttgcccgaa
     4621 tagaagatca tgccgccggg cgcgatcacc gggttccagt agagcgccgg ggcggcaaag
     4681 tcgggccggg tatcgtggtg ggggatgaac tccccgccgt aatgccgccc ttcggagacg
     4741 accggccagc catagttggc gccctgcttg accagattga gctcatcgcc gccggccggg
     4801 ccgtgctcga tttcccacag ccgcccgtcg ggcgcgaact gcatgccgag gatgttgcgg
     4861 tggcccaggc tgaaccgcac cggcgcgccg gcgggcttgc cagaggcgtc gatcggcagg
     4921 cggatcacct tgcccagatc gctggcggga tcctgcgcgg gctgcatctt ctggcggtcg
     4981 cccgaagcga tgaacagcgc cttgcggtcc gggctgatcg ccagccggtg cgaatagtgg
     5041 ccgcggccgg tgaccttggg gttctgccgc cagatcactt caaagccgtc cagggtcacg
     5101 gtgccgccgg tcatcttgag aacgccggtg cccacggccg cgccgcgggt atcgccttcg
     5161 ccagcttcgg catagctgag atagatccgg cccgacttgg caaagtccgg cgcggcgatc
     5221 acgtcgccca ggccgccctg gccgccataa gccacggtga agggtagggc gatctcgaca
     5281 acggtcttgt cggccttgcg cagcttcagc ttgccggact tttccgtcac cagcacgcca
     5341 ccatcgggca gcacggccag ggcccagggt tcctcgaacg tcgccagctc ttccgtggcg
     5401 aagtggcctt cgaccttttg gatcttcggc gggctatccc cggatgcggc gccgccacag
     5461 cttgcggcga tcaggacgag gggcgaaaag gccatcagga gattgttgcg catggttcgc
     5521 aatattggtg atggcggccg gtttgccaag tgccccgaag ggcaggtggt gatcggcgtg
     5581 gacgaggccg gacgcgggcc gctggccgga ccggtcgtgg ccgccgcctg cctgctgggg
     5641 ccgcacgcac cggcggggct ggacgattcc aagaagcttt cggccaagcg ccgcgcagcg
     5701 ctggagccgc agattcaggc cagctgccgc tgggcggtcg cggtggtgga agcggacgag
     5761 atcgaccgga tcaacatctt cgccgcaacc atgctggcga tgacccgcgc ggtcggcgcg
     5821 ctggtggccg aactggggca ggagccggac atggtcctga tcgacggcaa caagacgccc
     5881 gccggccggg tgccggaatg gcgctggacg gcccgcgcga tcatcggcgg cgacgcgctg
     5941 gagccttgca tttccgccgc ctcgatcctc gccaaggagc accgcgaccg cgtgatgcgc
     6001 gcccatgcag aggcccatcc gcactacggc tgggagcgca acatgggcta tggcaccgcc
     6061 gagcatctgg aggcgctgcg cgtccacggg ccaagcccga tccaccgcaa gagcttcgcc
     6121 ccggtggcgc agctggtgtt gctctaaatc ctcctcgaaa cggggagggg gaccatccgc
     6181 aggatggtgg aggggcacgc gagggtcatc ctgacttcac cagccctaac cggctgggtc
     6241 agccaaccgt gcccctccac tc
//
LOCUS       DASUDV010000216         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1751842,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000216 DASUDV010000000
VERSION     DASUDV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751842"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1246
                     /locus_tag="VFV30_04610"
     CDS             167..1246
                     /locus_tag="VFV30_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847401.1"
                     /translation="MLRDWRSLRVTLIACAVAAIVTAFQYQVYTSFVRAGAVGPKFVG
                     GSVWVSAGSIECFDFPGVIGEDYAALLARHFPDAGVRRVAFGFTGWTSPSGRRGNVAV
                     IGIDGAADGSGRRLPETAFMADRSDLSRLDLSGKPEEQATLGGETMTLAATTSQLPTF
                     LGAPYVVTSFEQARRILRMDPGSAAFLVFDSPQQGEDALAAALRQSQGQFPELTLRTG
                     AAFEQSSSVYWQRKTGAGSAIILAAILASLLMMILLANGVLRFLQRYSQDLVSMLGHG
                     AGQREIWLVTGGIAVIVAGVTLTAMMVFTPLMILAMKPLLPWVSFVAADALVPAGAVL
                     IALAVGIASAGSAVRQFGPEAVFRT"
     gene            1243..2232
                     /locus_tag="VFV30_04615"
     CDS             1243..2232
                     /locus_tag="VFV30_04615"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX4847402.1"
                     /translation="MKQLLALSLLALGACTASHSEPAAEAPGPYRATAVGRIDSAGEA
                     RQLVAAADGVIERVLVRRGDRVRAGQVLLEVGCAPRAGMAQASLAAARQSQAEAALVR
                     AGPRPEALAQGRAALAKAEADLRNAEQLLERAQALAGRGFVSRRELDARSAERDAARA
                     ARDGAAAQLAALSNGSRPQELASAQAAAQAAQGESAAARAALAQCQVRSPIDGQVLQV
                     LRREGEFSGASQGAPLIVVGDLDQRIVRAEVGERDVPHLRIGSPVEVWIDGSPKRWRG
                     RVVEMASVMGRRSARSLDPTDRFDRDVREAIVVIDDPDLPSVVGLRVTVGFVR"
     gene            2229..2885
                     /locus_tag="VFV30_04620"
     CDS             2229..2885
                     /locus_tag="VFV30_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4847403.1"
                     /translation="MSAQPLILEGLARSMLDSGQEKAMLAPLSRVFAPGQLHVVSGPS
                     GAGKTTLLSLLALAVPPTRGMIRWGSLNLSQLKAPVAAAWRRDNLGMIFQTSRLVSVM
                     NALENVCFGGAIRGMDVRGEAGRLLGAFGLGDKHDHLPAQLSGGEKQRVAIAQALACR
                     PALLLADEPTASLDAANAAFVAETLKSYARANAATVVCVSHDRTVIDAADDLLTLERA
                     "
     gene            2930..3769
                     /locus_tag="VFV30_04625"
     CDS             2930..3769
                     /locus_tag="VFV30_04625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4847404.1"
                     /translation="MHLIEQDQIEAAINLVVGKLHIAVDNVETWQEVRSCFQTHPLRQ
                     VLMEDPYVARCVAKPRGYAGDAELIDMLYRQVAPPGTTPRGKRAFAYSTNFPTSIAVR
                     QRKDLASKRLAKAVIDGDRVLCLACGHFREAEPLTGLDLQRVAVVDHDPASLEVVQSS
                     HDTVEVHVANVFAFLRKAVSSERRFDFVYTLGLTDYLDDRAMQLLHKLVRRLLEPGGT
                     FLLANFLPHHLAVGWMDAVMEWELIYREPEELEAFAVANQFTAKTWIDPTGSIAWCEM
                     HAI"
     gene            3783..>4088
                     /locus_tag="VFV30_04630"
     CDS             3783..>4088
                     /locus_tag="VFV30_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847405.1"
                     /translation="MHDQRFFDSIVGMIEADALDYAMPMLVGKLYGAYANREGWSQTR
                     STYMGHPLRAVMMEDPYIARCVTKPRGYAGDAELIDLIYDRRPPESVSARGLQIFRHG
                     "
BASE COUNT          623 a         1388 c         1391 g          686 t
ORIGIN      
        1 tgcgagggct agccgcaatc cgcgcgcgat ggcgtgacgg ccatcacggc cccggccggc
       61 cggcaagtcc ataaggccgc ccatgacaga tggtactgca actcaccgat ctgtagccga
      121 aacagtctcg caatgggccg cgcccgcggc ctttgtccgg gccgtcgtgc tgcgggactg
      181 gcgctcgctg cgggtgacgc tgatcgcctg cgcggtcgcg gcgatcgtca ccgcgttcca
      241 gtaccaggtc tacacctcgt tcgtccgggc cggcgcggtc gggccgaagt tcgtcggcgg
      301 gtccgtctgg gtcagcgccg gttcgatcga atgtttcgat ttcccgggcg tgatcggcga
      361 ggactatgcc gcgctgctgg cgcgccattt ccccgatgcc ggggtgcggc gggtggcctt
      421 cggctttacc ggctggacct cgcccagcgg gcggcgcggc aacgtggcgg tgatcgggat
      481 cgacggtgcc gccgatggca gcggccggcg cctgcccgaa accgccttca tggccgaccg
      541 ctcggatctg tcgcggctcg acctcagcgg caagcccgaa gagcaggcca cgctgggcgg
      601 cgagaccatg accctggccg ccaccaccag ccagctgccg acctttctgg gcgcgcccta
      661 tgtcgtcacc agttttgaac aggcccggcg gatcctgcgg atggatccgg gttcggccgc
      721 cttcctggtg ttcgacagcc cgcagcaggg cgaggacgcg ctggccgcgg cgctgcgcca
      781 atcgcagggc cagtttcccg agctgaccct gcgcaccggc gccgcgttcg agcagagctc
      841 gtccgtttac tggcagcgca agaccggcgc cggcagcgcg atcattctcg ccgcgatcct
      901 cgcctctctc ctgatgatga tcctgctggc caacggcgtg ctgcggttcc tgcagcgcta
      961 ttcgcaggac ctcgtctcga tgctcggcca cggtgccggc cagcgcgaga tctggctggt
     1021 gaccggcggg atcgcggtga tcgtcgccgg cgtcaccctg actgccatga tggtgttcac
     1081 cccgctgatg atcctggcga tgaagccgct gctgccgtgg gtttcgttcg tggcggccga
     1141 cgcgctggtt ccggccgggg ccgtgctgat cgcgctcgcc gtggggatcg ccagcgcggg
     1201 gagtgccgtg cgccaattcg gtccggaggc cgtgttcaga acatgaaaca actgcttgcc
     1261 ctctccctgc tggcgctggg cgcctgcacc gcatcccaca gcgagccggc cgccgaggcg
     1321 cccgggccct accgcgccac cgctgtcgga cggatcgaca gcgccggcga ggcgcgccag
     1381 ctggtcgccg cggccgacgg ggtgatcgag cgcgtgctgg tccgccgcgg agaccgggtc
     1441 cgggccgggc aggtcctgct cgaagtcggc tgcgccccgc gcgccgggat ggcccaggcc
     1501 tcgctcgccg cggcgcggca aagccaggcc gaggccgcgc tggtccgcgc cgggccgcgg
     1561 cccgaggcgc tggcccaggg ccgcgccgcc ctggccaagg ccgaagccga tctgcgcaac
     1621 gccgaacagc tgctggaacg ggcccaggcc ctggccgggc gcgggttcgt ttcgcgccgg
     1681 gaactcgatg cccgcagcgc cgaacgcgat gccgcccgcg ccgcgcgcga cggtgccgcg
     1741 gctcagcttg cggccctgtc caacggttcg cgcccgcagg agctggcctc ggcccaggcc
     1801 gcggcgcagg ccgcccaggg cgaatccgcc gccgcccgcg ccgccctggc acagtgccag
     1861 gtgcgaagcc cgatcgacgg ccaggtgctg caggtgctgc gccgcgaggg cgagttttcc
     1921 ggcgccagcc agggcgcgcc gctgatcgtg gtgggcgatc tggaccagcg aatcgtccgc
     1981 gccgaagtgg gcgaacgcga tgtgccccac ctgcggatcg gatccccggt ggaggtgtgg
     2041 atcgacggtt cgcccaagcg ctggcgcggg cgggtggtgg aaatggcctc ggtcatgggc
     2101 cggcgcagcg cccgctcgct cgatccgacc gaccggttcg accgcgacgt gcgcgaggcg
     2161 atcgtggtga tcgacgatcc ggaccttccc agcgtggtcg gcctgcgggt caccgtgggg
     2221 ttcgtgcggt gagcgcgcag ccgctgatcc tggaggggct ggcccgctcg atgctcgaca
     2281 gcgggcagga gaaggcgatg ctcgccccgc tcagccgggt cttcgcgccg ggccagctgc
     2341 acgtggtcag cgggccgtcc ggtgcgggca agaccacgct gctgtccttg ctggcgctgg
     2401 ccgtgccgcc cacccgcggg atgatccgct ggggcagcct caacctcagc cagctgaagg
     2461 ccccggtggc ggcggcctgg cggcgcgaca acctgggcat gatctttcag accagccggc
     2521 tggtttcggt gatgaacgcg ctggaaaacg tctgcttcgg cggggcgatc cggggcatgg
     2581 acgtccgcgg cgaagccggg cgactgctcg gcgcctttgg ccttggcgac aagcatgacc
     2641 acctgcccgc gcagctctct ggcggggaga agcagcgggt cgcgattgcc caggcgctgg
     2701 cctgccgccc ggcgctgctg ctggccgacg aaccgaccgc cagcctcgat gccgccaacg
     2761 ccgccttcgt ggccgagacc ctcaaatcct atgcccgcgc caacgccgcc accgtggtct
     2821 gtgtcagcca cgaccgcacg gtgatcgatg cggccgacga tcttttgacc cttgaaagag
     2881 catgagcgga gtgaggcgag tgaggcacgc tagattcttt gaccaagcca tgcacctgat
     2941 tgaacaggat cagatcgagg ctgcgatcaa cttggtcgtc ggaaagctgc acattgccgt
     3001 cgataatgtt gagacatggc aagaggtgcg cagttgtttc caaacacatc ccctacgtca
     3061 agttctgatg gaagacccat acgtggctcg ctgcgtagcc aaacctcgcg gctatgccgg
     3121 cgacgcagag ttgatcgaca tgctctatcg tcaggtcgct cctccgggca ccactccgcg
     3181 gggcaagcga gccttcgcct attcaacaaa cttcccgact tccattgccg tccgacagcg
     3241 aaaggacctc gcgagcaagc gactggcgaa agccgtgatc gatggtgacc gggtcctgtg
     3301 tcttgcctgc ggccactttc gtgaggcaga acctctgacc gggctcgact tgcaaagggt
     3361 ggcagtcgtt gaccatgatc cggcttcgct cgaagtggtg caatcttctc acgacaccgt
     3421 tgaagttcat gtcgccaatg tttttgcatt cttgcgaaag gccgtgtcat ccgagcggcg
     3481 ctttgatttc gtctataccc taggccttac agactatctc gacgaccggg caatgcagct
     3541 cttgcacaag ctggttcggc gattgcttga gcctggaggt acctttctgc tggcaaactt
     3601 cctgccacat caccttgcgg tcggctggat ggacgccgtg atggaatggg agctgattta
     3661 ccgcgagcct gaagagctcg aagccttcgc cgtggccaac caatttaccg caaagacatg
     3721 gatcgaccca accgggtcca tcgcatggtg cgagatgcac gcaatctaaa ggaacccaag
     3781 cgatgcacga ccaacgattc ttcgacagca tcgtcggcat gatcgaagcc gacgcgctcg
     3841 attatgccat gccgatgctg gtcggcaaac tttacggcgc ttatgccaac cgtgaaggct
     3901 ggtcgcagac ccgcagcacc tacatgggcc atccgctgcg cgcggttatg atggaggatc
     3961 cctacatcgc ccgctgcgtc accaagccgc gcggctatgc cggggatgcc gagctgatcg
     4021 acctgatcta tgaccgccgc ccgccggaga gcgtcagcgc gcggggcctg cagatcttcc
     4081 gccacggg
//
LOCUS       DASUDV010000217         8269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_492711,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000217 DASUDV010000000
VERSION     DASUDV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492711"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFV30_04635"
     CDS             complement(<1..242)
                     /locus_tag="VFV30_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX4847406.1"
                     /translation="MTRGARALIVYPEFRGRAAAAIDTAARLEEARGLALAIGLEVTD
                     AVAIPIREARAGTLFGEGQIQNIDIACNQGEAELVIV"
     gene            complement(269..850)
                     /gene="hfq"
                     /locus_tag="VFV30_04640"
     CDS             complement(269..850)
                     /gene="hfq"
                     /locus_tag="VFV30_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX4847407.1"
                     /translation="MTSRTLSARPRPPKPEPEAGEAAPAPVVTGGKGQNLQDQFLNLL
                     RKNKTPVTMFLVKGVKLQGIVTWFDNFSILLRRDGQSQLVYKHAVSTIMPSVPVDARQ
                     FGGGGEGGKKVRLLQDVFLTSVRNAEVQVTMFLVNGVMLQGRVAAFDLFCMLLEREGY
                     VQLAYKHAVSTIQPVTPVDLTGEGWDGDEGEDD"
     gene            complement(984..2387)
                     /locus_tag="VFV30_04645"
     CDS             complement(984..2387)
                     /locus_tag="VFV30_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4847408.1"
                     /translation="MALEILIVDDERDIRELVAGVLSDEGYQCRMAGDSSSALQMIDE
                     RRPSLVLLDVWLHGSPMDGLEVLDAIKSREPELPVIIFSGHGNIDTAVSAIGRGAMDF
                     IEKPFEAERLLLLVARATETERLRRENARLREGFVTSDEFTGNSAAINQVRATLKRVS
                     NTGSRLLISGPAGSGKEVAARLLHSWSPRSDHAFVTVNSARITPERFEQELFGEEANG
                     KLVRAGLLEMADGGTLYLDEVADMPLSTQARILRVLTEQSFVRVGGHRQIWVDVRVVS
                     STARDLEKEIADRRFREDLFYRLNVVPVSIPSLADRRDDIPMLVDHFFARFAADQGVA
                     APEVSSEAMAALQSYEWPGNVRQLRNVVERTIILTPRERLERIEADMLPGEVLSGRIS
                     GDNGLSALMGVPLREARENFEREYLRVQIRRFSGNISRTASFIGMERSALHRKLKLLG
                     MVDRREGEDEDEADAGS"
     gene            complement(2410..4632)
                     /locus_tag="VFV30_04650"
     CDS             complement(2410..4632)
                     /locus_tag="VFV30_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847409.1"
                     /translation="MAEAQVLKRAKRWPNWWRRLQIWARRANIIPVLEIGAVVAFLLM
                     TGLTWYKFTAPSAADKLMPTELTASLLVGTLVPAMAILVLLGRRLALKRAAERVGGGG
                     GGKLHVRLVFLFSLISSVPTLLVVIFASFLFQTGVDFWFSDQPRGLIKNANELAVGYR
                     EQIERDLEYESLAMAADLGDFLSREPISSPAFPEYYGYQIQQRKITESTILQKGNDGA
                     LHRAALARGPDPLSDQVSAGVLRQLDGGARIVVSSTGNSIQAVTPIDRRAGIYLYTAR
                     QSEFLSKDYTQNILRSYDELAHKARIQQFQFNVALFIASLALVGLSVWFALRFADRQV
                     EPLYDLVDAARQVGAGNFALRVEGRTGTDEIGLLNRAFNRMTQQLDKQTQALVGANQQ
                     LDERRAFIEAVLDSVTAGIISADARGRIRLINGSAQKLLLDRDAKPPIGRKLSDIAPE
                     IASLAESGREGGVVQYARDNDLLTLAVKATKASTGTVVTFEDITRQLLDQRQAAWSDV
                     ARRIAHEIKNPLTPIQLATERLKRRYTKLVSADAELFEELTGTIIRQVGDLRRMVDEF
                     SSFARLPKPMFRGEDPVDLARQALFLQEVARPDINFRFAADADMGIVACDRHQFGQAM
                     TNVLKNAVEAIEARQKVSEADYRGQVTVTLRGSKEDVVVTIADNGIGLPQDRERIVEP
                     YVTTREKGTGLGLAIVKKIVEEHGGDMAFAAGDPDGTVVTMRFARDPLSGQRSSEAAE
                     "
     gene            complement(4698..6101)
                     /locus_tag="VFV30_04655"
     CDS             complement(4698..6101)
                     /locus_tag="VFV30_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4847410.1"
                     /translation="MSVLLVEDDASIALVITAALEAEGFGVTVCDSIAERDRLLAGGC
                     YDALVTDVMLTDGDGIETIGAVRAAAPQMPIIILSAQNTLDTAVRASDTGAFEYFPKP
                     FDIDELARTVRQAVGNAAAEREGEIDAPVAGLPLVGRSAAMQAVYRMITRVLRNDLTV
                     LILGESGTGKELVAEAIHQLGQRKAGPFVAVNTAAIPADLIESELFGHEKGAFTGAVA
                     RHAGKFEQAGGGTLFLDEIGDMPLEAQTRLLRALQSGKIRRVGGREEIAVDVRIVAAT
                     NRDLEPMIAAGTFREDLYYRLNVVPITLPPLRERADDIPALAAHFLRLAADEGLPRHS
                     LSDEAATLLARQPWRGNVRELRNFIYRLALLVREDRIEAEALAPLLARAAASAEERDG
                     ARLDQAVEGWLADNRPPAGAVYEAALAAFERPLFVAALRETQGNQLRAAQLLGINRNT
                     LRKRLTDLGIDPAAIQR"
     gene            complement(6098..7183)
                     /locus_tag="VFV30_04660"
     CDS             complement(6098..7183)
                     /locus_tag="VFV30_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847411.1"
                     /translation="MVEAQRQLSSLSLAVVLLAPGQKLAAANPAAEQMLGQSFRRIEG
                     KALGDLLRFEEGRIADRLADSEAHVSARATGIVLTGQPPRKVDITVAPVVDQPGWQVM
                     TLVDIGGAEAMRDAPSAGEGGVLRAPEILAHEIKNPLAGIRGAAQLLGRKLEGKDLAL
                     TDLIADEVDRIAKLIDQMQSLSSRKALELAPCNLHEAVRRARAVIDAGGKAPPILEEF
                     DPSLPAVQGNADALVQVLINLLANAADATAAQDDRRIIIRTRFASGLQLHSSADGSAL
                     RLPIELRISDNGPGIDPELRDHVFEPFVTSKKSGQGLGLALVRRLLRDMNGRINHERD
                     EAGGWTHFRVHLAMAEERRKSLREEAA"
     gene            complement(7183..8199)
                     /gene="dusB"
                     /locus_tag="VFV30_04665"
     CDS             complement(7183..8199)
                     /gene="dusB"
                     /locus_tag="VFV30_04665"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX4847412.1"
                     /translation="MNPLPPSPGLAPIQVGPVGIDCPVILAPMTGVTDLPFRRMVRRF
                     GSGLNVTEMIASPAAIRETRVSLQKAMWDPAEEPVSMQLVGCDPVQMGEAAKLVEDRG
                     AAIIDINMGCPVRKVVNGDAGSALMRDVPLATKLIEATVNAVKVPVTVKMRMGWCHDS
                     LNAPELARIAEDLGAKMVTVHGRTRNQMYKGHADWAFVRAVKDAVSIPVIVNGDICEI
                     ADVAAALEQSGADGVMIGRGAYGRPWLLAQVMHWLKTGQQLADPDLETQLAVILEHYQ
                     AMLDHYGTDTGVKMARKHLGWYTRGLRDSAQFRNMINFVDDAEQVMRLLREFYEPFLT
                     KAAA"
BASE COUNT         1316 a         2828 c         2723 g         1402 t
ORIGIN      
        1 acaatcacca gctcggcctc gccctggttg caggcgatgt cgatgttctg gatctggcct
       61 tcgccgaaca gggtgccggc ccgcgcctcg cggatcggga tagcgaccgc atcggtcact
      121 tccagcccga tagccagggc caggccgcgc gcctcctcaa gccgtgccgc ggtgtcgatc
      181 gcggcggcgg cccgcccccg gaattccggg taaacgatca gcgcccgcgc cccgcgggtc
      241 acgtcctcgc ggttgtcgtc aagaaaactc agtcgtcttc gccttcgtcg ccatcccagc
      301 cctcaccggt caggtcgacc ggggtgacgg gctgaatggt ggagaccgcg tgcttgtagg
      361 ccagctggac gtaaccttcg cgctccagca gcatgcagaa cagatcgaac gcggcgaccc
      421 ggccctgcag catgacgccg ttgaccagga acatggtgac ctggacttcg gcgttgcgga
      481 cgctggtcag gaacacgtcc tgcagcaggc gcaccttttt gcccccttcc ccgccgccgc
      541 cgaactggcg ggcgtcgacc ggaacgctgg gcatgatggt ggaaacggcg tgcttgtaga
      601 ccagctgact ctgcccgtcg cggcgcagca ggatcgagaa attgtcaaac caggtgacga
      661 tgccctgcag cttcacgccc ttgaccagga acatggtgac cggggtcttg ttcttgcgca
      721 gcagattgag gaactggtcc tgcaggttct gccccttgcc gccggtcacg accggggcgg
      781 gtgcggcttc gccggcttcg ggttcgggct tgggcgggcg cgggcgtgcg ctaagcgtgc
      841 gtgaggtcat ctgccttctc catctttttg gcggcctgtt gccagagcca caatctggtc
      901 gttccgatgg aatcaaccgg ttcaatgacg cgcttattac cgtgctttcg cgccgtggta
      961 aacgcccaac cggacaggcc cggtcaggat ccggcgtccg cttcgtcctc gtcctcgccc
     1021 tcgcgccggt cgaccatgcc gagcagcttg agcttgcggt gcagcgccga gcgctccatc
     1081 ccgatgaaac tggcggtgcg cgagatgttg ccggagaacc gccggatctg gacccgcagg
     1141 tactcccgct cgaaattctc tcgcgcctcg cgcagcggca cgcccatcag cgcggagagg
     1201 ccgttgtcgc cgctgatccg gccggacagg acctcgcccg gcagcatgtc ggcctcgatc
     1261 cgctcaagcc gctcgcgcgg ggtgaggatg atcgtgcgtt ccaccacgtt gcgcagctgg
     1321 cgcacgttgc cgggccattc ataactttgc agcgcggcca tcgcttcgga cgaaacctcg
     1381 ggcgcggcca cgccctggtc ggcggcaaag cgcgcgaaga agtgatcgac cagcatcgga
     1441 atgtcgtcgc gccggtcggc caggctgggg atcgagaccg gaaccacgtt gagccggtag
     1501 aacaagtctt cgcggaaccg ccggtcggcg atttcctttt ccagatcgcg cgcggtggaa
     1561 gacaccacgc ggacatcgac ccagatctga cggtgcccac ccacccggac gaaggactgc
     1621 tcggtcagca cccgcaggat ccgggcctgg gtggacagcg gcatgtccgc cacctcatcg
     1681 aggtacagcg tcccgccatc agccatttcc agcagacctg cgcggaccag cttgccgttg
     1741 gcctcttccc cgaacagctc ctgctcaaag cgctcgggcg tgatccgggc ggaattgacc
     1801 gtaacgaagg catggtcgga acgcgggctc cacgaatgga gcagccgcgc cgccacttcc
     1861 ttgcccgatc cggccgggcc cgaaatcagc aggcggctgc cggtgttgga gacccgcttc
     1921 agcgtggcgc gcacctggtt gatcgcggcc gaattgccgg tgaactcgtc ggaggtgaca
     1981 aagccttccc gcaggcgggc gttctcgcgc cgcagccgct cggtctcggt cgcgcgggcc
     2041 accagcagca gcaggcgttc ggcttcaaag ggcttctcga tgaaatccat cgccccgcgc
     2101 ccgatcgcgg aaacggcggt gtcgatgttg ccgtggccgg agaagatgat caccggcagt
     2161 tccggctcgc ggctcttgat cgcgtcgagc acttcaagcc catccatcgg gctgccgtgc
     2221 agccacacgt cgagcagcac caggctgggc cggcgctcgt cgatcatctg cagggccgaa
     2281 ctgctgtctc cggccatgcg gcattgatag ccttcatcgc tcagcacgcc ggccacgagc
     2341 tcgcggatgt cgcgttcgtc gtcgacgatc aggatttcga gcgccattct gttctacctc
     2401 ggtttcattc tattcggccg cttccgaaga ccgttggccc gaaagcggat cgcgggcgaa
     2461 gcgcatggtg acaacggtcc cgtcgggatc gccggcggcg aaggccatgt cgccgccgtg
     2521 ctcctcgacg atcttcttca cgatcgcgag acccagtccg gtccccttct cgcgggtggt
     2581 gacatagggt tcgacgatcc gttcgcggtc ctggggcaga ccgatgccgt tatcggcaat
     2641 cgtcaccacc acgtcctcct tggatccgcg cagggtcacg gtcacctggc cgcggtaatc
     2701 ggcctcgctg accttctgcc gcgcctcgat cgcctcgacc gcattcttga ggacgttggt
     2761 catggcctgg ccgaactggt gccggtcgca agcgacgatc cccatgtccg catcggcggc
     2821 gaagcggaag ttgatgtcgg ggcgggccac ttcctgcagg aacagcgcct gccgcgcgag
     2881 gtcgaccgga tcctccccgc ggaacatcgg cttgggaagc cgcgcgaacg aggagaattc
     2941 gtcgaccatc cggcgcagat ccccgacctg gcggatgatc gtgccggtga gctcctcgaa
     3001 cagttcggca tcggccgaga ccagcttggt atagcggcgc ttcagccgct cggtcgccag
     3061 ctggatcggg gtcagcgggt tcttgatctc gtgcgcgatc cggcgggcca catcggacca
     3121 ggccgcctgg cgctgatcga gcagctggcg ggtgatgtcc tcaaaggtga cgacggtgcc
     3181 ggtgctggcc ttggttgcct tgactgccag ggtcagcagg tcgttgtcgc gggcgtactg
     3241 caccaccccg ccctcgcgcc cgctttcggc caggctggcg atttcggggg caatgtcgga
     3301 gagcttgcgg ccgatcggcg gcttggcatc gcggtccagc agcagcttct gggccgaccc
     3361 gttgatcagc cggatccgcc cgcgcgcatc ggccgaaata atcccggcgg tgacggaatc
     3421 gagcaccgcc tcaataaagg cccggcgttc atccagctgc tggttcgcgc cgaccagcgc
     3481 ctgggtctgt ttgtcgagct gctgggtcat gcggttgaag gcgcggttga ggaggccgat
     3541 ttcatcggtc ccggttcgcc cttcaacgcg cagcgcgaag ttgcctgccc ccacctgacg
     3601 cgccgcatcg accagatcgt agagtggctc cacctgccgg tcggcaaaac gcagcgcaaa
     3661 ccacaccgac agaccgacca gggccaggct ggcaatgaac agcgcgacgt tgaactggaa
     3721 ctgctggatc cgggccttgt gggccagctc gtcataagag cgcaggatgt tctgggtgta
     3781 atccttgctg agaaattccg attgccgcgc ggtgtagagg tagatcccgg cgcgccggtc
     3841 gatcggggtt accgcctgga tcgagttgcc ggtcgaactg accacgatcc gcgccccgcc
     3901 gtccagctgg cgcagcaccc cggccgagac ctgatcggac aacgggtcgg gcccgcgggc
     3961 cagcgcggcg cggtgcagcg cgccgtcgtt gcccttctgc aggatggtcg attcggtgat
     4021 cttgcgctgc tggatctggt atccgtagta ctccgggaag gccgggctgc tgatcggctc
     4081 gcggctcagg aagtcaccaa ggtccgccgc catggccagc gattcatact ccagatcgcg
     4141 ctcgatctgc tcgcggtagc cgaccgccag ctcgttcgcg ttcttgatca ggccgcgcgg
     4201 ctggtccgag aaccagaagt ccaccccggt ctggaacagg aacgaggcga agatcaccac
     4261 cagcagggtc ggaacggacg agatcagcga gaacaggaac accaggcgga cgtggagctt
     4321 gccgcccccg ccgccgccaa cccgctccgc cgcgcgcttc agcgccaggc gccggccgag
     4381 cagcaccagg atcgccatgg ccggaaccag cgtgccgacc agcagcgagg cggtcagttc
     4441 ggtcggcatc agcttgtcgg cggccgaagg tgcggtgaac ttgtaccagg tcagcccggt
     4501 catcagcagg aaggccacga ccgcgccgat ttccagcacc ggaatgatgt tggcccgccg
     4561 cgcccagatc tggaggcggc gccaccagtt cggccagcgc ttggcgcgct tcaggacttg
     4621 ggcctctgcc atcgttgcgc gattacaaca cccctgttgc aagagtgcaa ggggattcgc
     4681 cgcgcgcccg gcgcgcttca gcgctggatg gccgccggat cgatacccag gtcggtcagg
     4741 cgcttgcgca gggtgttgcg gttgatcccc agcagttggg ccgcgcgcag ctggttgccc
     4801 tgggtctcgc gcagcgcggc cacgaacagc gggcgctcaa acgccgccag cgccgcttca
     4861 tagactgccc cggccggcgg tcggttgtcg gccagccagc cctcgaccgc ctgatcgagc
     4921 cgggccccgt cgcgctcttc ggccgacgcg gcggcccggg ccagcagcgg ggccagcgcc
     4981 tcggcctcga tccgatcctc gcgcaccagc agcgcgagcc ggtaaatgaa attgcgcagc
     5041 tcgcgcacgt tgccgcgcca gggttggcgc gcgagcaggg tcgcggcctc gtcgctcagg
     5101 ctgtggcgcg gcaggccctc gtccgctgcc aggcgcagga aatgcgcggc cagagccgga
     5161 atgtcatccg cccgttcgcg cagcgggggc agcgtgatcg gcaccacgtt gagccggtaa
     5221 tagagatcct cgcgaaaggt tccggcggca atcatcggtt ccagatcgcg gttggtggcg
     5281 gccacgatgc ggacatcgac cgcgatttcc tcgcgcccgc cgacccggcg gatcttgccc
     5341 gattgcagcg cgcgcagcag ccgggtctgg gcctccagcg gcatgtcgcc gatctcgtcg
     5401 aggaacaggg tgccgccgcc ggcctgttcg aacttgccgg catggcgggc caccgcgccg
     5461 gtaaaggcgc ccttctcgtg cccgaacagt tcgctctcga tcaggtcggc cgggatggcc
     5521 gcggtgttga ccgcgacaaa cggcccggcc ttgcgctggc ccagctggtg gatcgcctcg
     5581 gcgaccagtt ccttgccggt tccgctttcg cccaggatca gcacggtcag atcgttgcgc
     5641 agcacccggg tgatcatccg gtaaaccgcc tgcatcgccg ccgagcggcc gaccagcggc
     5701 aggccggcga ccggggcgtc gatctcccct tcccgctcgg ccgccgcatt gccgaccgcc
     5761 tggcgcacgg tgcgggccag ctcgtcaatg tcgaacggct tgggaaaata ctcgaacgcg
     5821 ccggtatcgc tggcgcggac cgcggtatcg agggtgttct gcgccgagag gatgatgatc
     5881 ggcatctgcg gcgccgcggc gcggaccgca ccaatcgttt cgatcccgtc cccgtcggtc
     5941 agcatgacgt cggtcaccag cgcgtcatag caccctccgg ccagcagccg gtcccgctcc
     6001 gcgatcgaat cgcagacagt gacgccaaac ccctcggcct ccagcgcggc ggtgatcacc
     6061 agcgcgatcg aggcatcgtc ttcgaccagc aggacgctca tgccgcctcc tcgcgcagcg
     6121 acttgcggcg ctcttccgcc atggcgaggt ggacgcggaa atgcgtccag cctccggcct
     6181 cgtcgcgttc gtggttgatc cgcccgttca tgtcgcgcag caaccgacgg accagggcca
     6241 gacccagacc ctgcccgctc ttcttcgagg tgacgaaggg ctcgaacacg tgatcgcgca
     6301 gctccggatc aatgcccggg ccgttgtcgc tgatcctcag ctcgatcggc agccgcaggg
     6361 ccgaaccgtc ggcgctggaa tggagctgca gcccgctggc aaagcgggta cggatgatga
     6421 tccggcggtc gtcctgcgcg gccgtggcat cggcggcgtt ggccagaagg ttgatcagca
     6481 cctggaccag cgcatcggcg ttgccctgaa ccgccggcag ggacggatcg aactcctcga
     6541 ggatcggcgg agctttcccc ccggcgtcga tcaccgcgcg ggcccggcgc accgcctcat
     6601 gcaggttgca gggcgccagt tccagtgcct tgcggctgga gagcgactgc atctggtcga
     6661 tcagcttggc gatccggtcc acctcgtcgg cgataagatc ggtcagggcc aggtccttgc
     6721 cctccagctt gcggcccaga agctgtgcgg cgccgcggat cccggccagc gggttcttga
     6781 tttcatgggc taggatttcc ggcgcgcgca gcaccccgcc ctcgccggca gagggcgcat
     6841 cgcgcatcgc ctcggccccg ccgatgtcga ccagggtcat cacctgccag cccggctggt
     6901 cgaccaccgg cgctacggtg atgtccacct tgcgcggagg ctgtccggtc aggacaatcc
     6961 cggtcgcccg ggcggaaaca tgggcttcgc tgtcggccag acggtcggca atccggcctt
     7021 cctcgaaccg cagcaggtcg cccagcgcct tgccctcgat ccggcgaaag ctttgcccca
     7081 gcatctgctc cgccgcgggg ttggccgccg ccagcttttg cccgggggcc agcagcacca
     7141 ccgccagcga caggctggaa agctggcgct gcgcctcgac catcaggccg ccgccttggt
     7201 caggaacggt tcgtagaatt cgcgcagcag gcgcatcacc tgctcggcat cgtcgacgaa
     7261 gttgatcatg ttgcggaact gggcggaatc gcgcagcccc cgggtgtacc agcccagatg
     7321 cttgcgggcc atcttgaccc cggtgtcggt accgtagtga tccagcatgg cctgatagtg
     7381 ttccaggatc accgccagct gggtttccag atcgggatcg gccagttgct gcccggtctt
     7441 cagccagtgc atcacctgcg ccagcagcca cggacggcca taggccccgc gcccgatcat
     7501 gacgccatcc gcgcccgact gctccagcgc ggcggccaca tcggcaatct cgcaaatgtc
     7561 gccgttgacg atcaccggga tcgagaccgc atcctttacc gcgcggacaa aggcccagtc
     7621 ggcgtggccc ttgtacatct ggttgcgggt gcggccgtgg acggtgacca tcttcgcgcc
     7681 aaggtcctcg gcaatgcggg ccaattcggg ggcgttcagg ctatcatggc accagcccat
     7741 gcgcatcttg accgtgaccg gcacctttac cgcattgacg gtcgcctcaa tcaatttcgt
     7801 cgccagcggc acgtcgcgca tcagggcaga gccggcatcg ccgttcacca ccttgcgcac
     7861 cggacagccc atgttgatgt cgatgatcgc cgcgccgcgg tcttcgacca gcttggccgc
     7921 ctcgcccatt tgcaccggat cgcagccgac cagctgcatc gaaaccggct cttccgcagg
     7981 gtcccacatc gccttttgca gcgaaacgcg ggtttcgcgg atcgcggccg ggctggcgat
     8041 catttcggtg acgttgaggc ccgagccgaa ccggcggacc atccggcgga acggcaggtc
     8101 ggtcacgccg gtcatcggcg cgaggatcac cgggcagtcg atgccgacgg ggccgacctg
     8161 gatcggggca agcccgggag agggaggaag cgggttcatg gatgattgcc taaaatttgg
     8221 gcagcacata gggaattgct ccgcgcgcgg caagcctata ccggcatgg
//
LOCUS       DASUDV010000218         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1133363,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000218 DASUDV010000000
VERSION     DASUDV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133363"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFV30_04670"
     CDS             complement(<1..829)
                     /locus_tag="VFV30_04670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4847413.1"
                     /translation="MTGAASGESLAAIFRRLIANTGPISLMHYMGEANARYYSSRDPL
                     GSGGDFITAPEISQMFGELIGLWLADMWINAGREEPVHYVELGPGRGTLAKDALRAAR
                     RYGLNPSIHFVEASTELKDVQLATVPEANWHPDLSSVPMYGPVLVVANEFLDALPVRQ
                     LVKTPEGWRERMVIPEGERFACVAGSQPMDAAVPEARRNAADGTIIETCPGAAATVYE
                     VAGRLLEQGGAALFIDYGHDTIRDGSSLQAVRQHLKVDPFINPGEADLTAHVDFATLA
                     "
     gene            complement(826..1683)
                     /gene="lgt"
                     /locus_tag="VFV30_04675"
     CDS             complement(826..1683)
                     /gene="lgt"
                     /locus_tag="VFV30_04675"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4847414.1"
                     /translation="MLSLLLAAAADAPLQWRDLGLRPEVFSIGGFALRWYSLAYLAGI
                     ILGYWHLSKMIKAPGAPMAQRHADDLFFYCTLGIILGGRLGYATFYDPDLWANPLDLL
                     KLWNGGMSFHGGLMGVVLAIAWVSRSGQLSFLRVCDYIAVCVPFGMLFGRLANFNNGE
                     LWGRVADVPWAMVFPGAGDLPRHPSQLYEAALEGALLAVVLIWLFWKTRARWKPGLLV
                     GLFTLGIGLGRFVVEYFREPDVQLAEFAQRTGLSMGQWLTIPLMLVGAFFLIRALRGP
                     ELGSGTPQA"
     gene            1827..>2622
                     /locus_tag="VFV30_04680"
     CDS             1827..>2622
                     /locus_tag="VFV30_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX4847415.1"
                     /translation="MASELDNALKSVLERLTAPGGPLETVPIERFGRTLPMLKFAPPT
                     LRDYFDLFCAQHGSAVFLVDGDIRMTFAEVHAAARTVAGGLVAGHGLEKGERVGIAAR
                     NSVNWIVAYLGVLMAGGCATLLNGWWSGEELAGGIELAGCKMTLADEGRAKRLADQTY
                     PGKLVLIEHDCPWQEGLAALLERGGGADTPLPALTGDDLATILYTSGSTGQSKGAYSD
                     HRGVVQATMNYAAQSLMVLTHMVDKGEGPPEGYQPCTLVNVPLFHVT"
BASE COUNT          498 a          870 c          847 g          407 t
ORIGIN      
        1 gggcgagcgt cgcgaaatcg acgtgcgcgg tcagatcggc ctcgcccgga ttgatgaagg
       61 gatcgacctt gaggtgctgg cgcaccgcct gcaggctcga tccatcgcgg atcgtgtcgt
      121 ggccataatc gatgaacagc gccgcgccgc cctgttcaag caagcgtccg gccacttcat
      181 agaccgtggc cgccgcgccg ggacaggttt cgatgatcgt gccatcggcc gcattgcgcc
      241 gcgcctcggg tacggcggca tccatcggct ggctgccggc cacgcaggca aagcgttcgc
      301 cctcggggat caccatgcgc tcgcgccagc cctcgggggt cttgaccagc tggcggaccg
      361 gcagcgcatc aaggaactcg ttcgccacca ccagtaccgg cccgtacatt ggcacgctcg
      421 acaggtcggg atgccagttg gcttcgggca cggttgccag ctgcacgtcc ttgagctcgg
      481 tcgatgcctc gacaaaatgg atcgaagggt tgaggccata acgccgcgcg gcgcgcagcg
      541 cgtccttggc cagggtcccg cggcccgggc ccagctcgac gtagtggacc ggttcctcgc
      601 gcccggcgtt gatccacatg tcggccagcc acagcccgat cagttcgccg aacatctggc
      661 tgatttcggg cgcggtgatg aaatcgccgc cgctgcccag cggatcgcgg ctggaatagt
      721 agcgggcgtt ggcctcgccc atgtaatgca tcaggctgat cggcccggtg ttggcaatca
      781 accggcggaa gatcgcggcc aggctttcgc cacttgcggc tccggtcatg cctgcggcgt
      841 gccgctaccc agctcggggc cacgcagcgc ccggatcagg aagaaggccc ccaccagcat
      901 cagcgggatg gtcagccact ggcccatcga aagcccggtg cgctgggcga attcggccag
      961 ctgcacatca ggttcgcgga aatattcgac cacaaaccgg cccagcccga tccccagggt
     1021 gaacagtccg accagcagcc ccggcttcca gcgcgcgcgg gtcttccaga acagccagat
     1081 cagcaccact gccagcagcg cgccttcaag cgcggcttca taaagctggc tgggatggcg
     1141 cggcaggtcg ccggcgccgg ggaacaccat ggcccagggc acgtcggcaa cccgccccca
     1201 cagctcgccg ttgttgaaat tggccagccg cccgaacagc atcccgaacg gcacgcagac
     1261 cgcgatgtaa tcgcacaccc gaaggaacga gagctgccca ctgcgcgata cccaggcgat
     1321 cgccagcaca acgcccatca agccgccatg aaagctcatg cccccgttcc aaagcttgag
     1381 caggtccagc ggattggccc acagatcggg atcgtagaag gtggcatagc ccagccgtcc
     1441 tcctaggatg atccccaggg tgcaatagaa gaacagatcg tcggcatggc gctgggccat
     1501 cggtgcacca ggcgccttga tcattttcga gaggtgccag tagcccagga tgatccccgc
     1561 cagataggcc aggctgtacc agcgcagggc gaatccaccg atcgagaaaa cctcgggacg
     1621 caggcccaga tcgcgccatt gcagcggagc atcggcagcg gcagcaagca gtagtgacag
     1681 caaggactta accccctaaa cgcgattcaa acgcggtaag cgcgtggtgc cggatgtctt
     1741 ggcacaggaa acgccgggag acaaacccgg cacaaccatg gatgcgataa gggggcgcgt
     1801 ccgcgacttg ggaagaagga tgccgcatgg cgagcgaact cgataatgcc cttaaatccg
     1861 tactcgaacg tctgaccgcc ccggggggac ccctggaaac cgtaccgatc gaacgctttg
     1921 gcaggaccct gccgatgctc aagttcgcac cgccgacgct gcgcgactac ttcgacctgt
     1981 tctgcgcgca gcatggcagc gcggtcttcc tggtcgatgg cgatatccgg atgacctttg
     2041 ccgaagtcca cgccgccgcg cggaccgttg ccggcgggtt ggtggccgga cacgggcttg
     2101 aaaagggcga gcgggtgggg atcgccgcgc gcaattcggt caactggatc gtggcctatc
     2161 tgggcgtact gatggccggc ggctgcgcaa cgctgctcaa cggctggtgg tcgggcgagg
     2221 aactggccgg cgggatcgag cttgccggct gcaagatgac gctggccgac gaaggccggg
     2281 ccaagcgcct ggccgaccag acctatcctg gcaagctggt gctgatcgaa catgattgcc
     2341 cctggcagga gggccttgcc gccctgctcg aacgcggggg cggtgccgat acccccttgc
     2401 ccgcgctgac cggcgatgat ctcgccacga tcctctatac ctcgggctcg accggccagt
     2461 cgaagggcgc ctattcggat caccgcgggg tggtgcaggc aacgatgaac tatgccgccc
     2521 agtcgctgat ggtgctcacc cacatggtcg acaagggcga aggtccgccc gaaggctatc
     2581 agccctgcac cctggtcaac gtgccgctgt tccacgtcac cg
//
LOCUS       DASUDV010000219         6781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_824147,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000219 DASUDV010000000
VERSION     DASUDV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824147"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VFV30_04685"
     CDS             complement(<1..1546)
                     /locus_tag="VFV30_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX4847416.1"
                     /translation="MLDRTGAPLDPADLTAMGLAATGSGTAVTGRDAQDPHLVSTLSD
                     SDGLTVLSGWIADRDELAQRLGLQPGAPLAALARAALLRFGAETPDEMLGEWSLFHTG
                     ADGAAWLMQGATTRDWLFFAHRPGWLAFAPDAEALARLKGVDAALDPQVIGLSLASYK
                     LRAQLGSRSIYKGIEKLHPGCSVQIAPDGAVQRHAVEMLRPEPRFTGTANEALEALEE
                     ALRRILRDRLGRTPRPVLLLSGGLDSSLLAALAAQDMGERPLALCSVAPPGSGLADEV
                     EFARAVADRHGIELIPVSPESDADPYRPARHVIAGAECPLLNNRHCLTSRFHEVARHN
                     GGTMLVNGTYGEMSVAARLPGPPSLRNRLGALRRWLASAWHREQADFHAKLAPHRYAE
                     LARLSRDLPQPVIAAQGPPDQIGYTPGVEKALVQTNAYYAGALRMDLPYRDLRLLRLY
                     ASLPRALAYELGPNRGPARTIGKGILPEQVRQRQRGMPADPGHFARLQAFAEPARSRI
                     GAFRAGG"
     gene            1635..2555
                     /locus_tag="VFV30_04690"
     CDS             1635..2555
                     /locus_tag="VFV30_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847417.1"
                     /translation="MEAAFDNDIRRAVRASLAREAYAKGKVRLTNPDLGDSWWLVTTH
                     RGLYSASPDGIQLVSHGWYFGVDRHGDHIYLYENCGLRDRESHLGRIVRLHWDGRHLS
                     DPAVLVTGLHGNAHQLKVIGGLLCLLDTANQRILRFTLDGAPVDTKTPLPPAPANDTS
                     GAYHHINSIAEIGGRVAILLHNGKAPSPRKSELVWLDADWQVAGRGEIDGYMCHDIVA
                     DGDGTLWHCASASGELTGSNGERIKLSDSLMTRALSFCRDHLLVGLSSFGERQIRDGL
                     SGEVLVLDRDRRVVAHHDVPAGPADVVALR"
     gene            2552..3307
                     /locus_tag="VFV30_04695"
     CDS             2552..3307
                     /locus_tag="VFV30_04695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX4847418.1"
                     /translation="MTGLGPDLDAVKRMVTGFDRLGGSGDFARFVPLSLYDAKAAAWT
                     VARKDLPLTLLTKAQLDLVEELLVGIGQRGVPGDCIEAGVWRGGTVILMRAVLDAHGM
                     ADRQVIAADSFAGIPPSTRFLHDPVDLWPDRWIASLEEVQANIAGAGMGDPRIEFVEG
                     LFADSLPRLAGRQLAFARLDSDSHDSVMDSLTWLYPLLMPGGAILIDDWHLFGCRLAV
                     DRYRHQHGITDPIQTVARNGYWFKSQPWGQPAP"
     gene            complement(3382..3525)
                     /locus_tag="VFV30_04700"
     CDS             complement(3382..3525)
                     /locus_tag="VFV30_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847419.1"
                     /translation="MTQPNQKTERKTWMEPEIRELDIRETFAFPGRGADIGGNPSIDC
                     QRS"
     gene            complement(3894..4037)
                     /locus_tag="VFV30_04705"
     CDS             complement(3894..4037)
                     /locus_tag="VFV30_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847420.1"
                     /translation="MTQPDQNTERKTWVEPTIRELEIRDTFAFPGRGADIGGNAFIDC
                     QRS"
     gene            4260..5414
                     /locus_tag="VFV30_04710"
     CDS             4260..5414
                     /locus_tag="VFV30_04710"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX4847421.1"
                     /translation="MTLAEERALARQAYERAGSALWRLRGAQLATQDDDYAEVVTLLE
                     QADDLEFSERLMLMRAYLAMETPADTERARLTAEAAVAVADLPLQRAAALADRAKAER
                     RLSLARAARGSLEAALADDPHNKDACKRLAALDLEEGRAPQLVDWCKSLTGSGVGHAR
                     LHAAHVLGEAARGDLDAARAVEGFAALSESAILPPPPGWSDLAAFNAALAAELLGHRN
                     MRYERYGSASNYTWRIDWLARPETPLVRVLLDHLAGHIQARVGRLDRADHRWLADKPE
                     RALLRAWCVITEGDGFEGWHVHQFGWLSGVYYVQIPEEIALGRDLGGCLGFGLPDDLS
                     GQAVSDAYGTTVIRPEPGMMVTFPSHTFHRTFAHHGPGKRICIAFDVKPA"
     gene            5435..6079
                     /locus_tag="VFV30_04715"
     CDS             5435..6079
                     /locus_tag="VFV30_04715"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX4847422.1"
                     /translation="MHLPVPFLIGSQGVTVQGDPAPWRDAFASLPAIAIPAAIEPAFC
                     ARMVERAAQAHFAEDDVAYIGTRAIEEPQRVGRALSLLLHSPELLHWLEQATGTGTLR
                     AVAGRLVETRANHRDALDWHDDRDDLTRRLAIVIDLSDQRYQGGQFQLRRKGEAEPLL
                     SHDHAQAGSVLIFAVRPELEHRVTPVLAGGPRRVFAGWFLTQPEHASGALGASG"
     gene            complement(6071..>6781)
                     /locus_tag="VFV30_04720"
     CDS             complement(6071..>6781)
                     /locus_tag="VFV30_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847423.1"
                     /translation="EDEVSFEIHGGGAIGWIPGPDWNGRLPVSFFSSVAAITLAWRGL
                     LPLHASTVVLHGKAWLIAGSPGAGKSTLTAELLAAGAELLADDLTALSPSPQPLAWRG
                     RPAMRLHPASCAGLAIEGPPEPIDDGRGKVLVRPSARAADRGWPVGGLILLGQIIGNG
                     PTAADKAMAYGSVLFRPRILSALPGRAAIREGLLGLARTVPMALVPEAWAFDPEARAR
                     RVDQALTAIARMAAKASA"
BASE COUNT         1050 a         2248 c         2378 g         1105 t
ORIGIN      
        1 taccgcccgc gcggaaggcc ccgatccggc tccgcgccgg ttccgcgaaa gcttgcagcc
       61 gggcaaagtg gcccggatcg gccggcatgc cgcgctgacg ctggcgcacc tgttcgggaa
      121 ggatgccctt gccgatcgtg cgggccgggc cgcgattggg ccccagctca taggccagcg
      181 cgcgaggcag gctggcatag agccgcagca gccgcagatc gcggtagggc aggtccatcc
      241 gcagcgcacc ggcgtaatag gcattggtct ggacaagggc cttttcgacg ccgggcgtat
      301 agccgatctg atccggcggg ccttgggccg caatgaccgg ctggggcagg tcgcggctca
      361 gtctggccag ttcggcgtag cggtgcggtg ccagcttggc gtgaaagtcc gcttgctcgc
      421 ggtgccaggc cgatgccagc cagcgccgca gcgcgccaag gcgattgcgc aagctcggcg
      481 gtccgggaag acgggcggca accgacattt cgccgtaggt tccgttgacc agcatggttc
      541 cgccgttatg ccgggccacc tcgtgaaagc ggctggtcag gcaatgccgg ttgttcagca
      601 aggggcattc cgcccccgcg atcacatggc gggcggggcg atagggatcg gcatcgctct
      661 ctggcgaaac cgggatcagt tcgatcccgt gccgatccgc aacggcgcgg gcaaattcga
      721 cttcgtcggc aaggcccgag ccgggcggcg cgaccgagca cagcgccagc ggcctttccc
      781 ccatgtcctg agcagccaag gcggccagca gcgaggaatc gaggccgccg ctcagcagca
      841 ggaccggccg cggtgtgcgg cccaggcggt cgcgcaggat acgccgcagg gcttcctcca
      901 gcgcttccaa ggcctcgttc gcggttcccg taaaacgggg ttcgggacgg agcatctcaa
      961 ccgcatggcg ctgcaccgca ccgtccggtg cgatttgcac actgcatccc ggatggagct
     1021 tttcgatccc cttgtagatc gaacgcgagc caagctgggc acgcagcttg taactggcga
     1081 gggacaggcc gatgacctgc ggatcgagcg ccgcgtcgac acccttcagc ctggccagcg
     1141 cctcggcatc gggggcaaag gccagccagc ccgggcggtg ggcgaagaac agccagtcgc
     1201 gcgtggtggc gccctgcatc agccaggcgg caccatcggc cccggtgtga aacagcgacc
     1261 attcacccag catttcgtcc ggcgtctccg caccgaatcg cagcagggcc gcgcgggcga
     1321 gggcagccag aggggcaccc ggttgcaggc ccagccgctg ggccagttcg tcccggtcgg
     1381 caatccagcc gctcagcacg gtcagcccgt cgctgtcgga aagcgtgctg accagatgcg
     1441 ggtcctgcgc atcgcgcccg gtgacagccg tgccgctgcc ggttgccgcc agacccatcg
     1501 ctgtcaggtc tgcgggatcg agcggggcgc cggttctgtc gagcacaccc cacagtcccg
     1561 atcggagcgg gtcagccatg gcaaagaccg tctgcggcct tgcctatcgg ccacgctgcg
     1621 ataggggttg ggcgatggaa gcggcctttg acaacgacat ccgccgcgcg gtccgcgcga
     1681 gcctggcccg cgaagcctat gccaagggga aggtgcgtct cacgaacccc gatctgggcg
     1741 atagctggtg gctggtgacg acacatcgcg gcctctattc ggcgtctccc gatggaatac
     1801 aactggtttc gcacggctgg tactttggcg tcgatcgcca cggggaccat atctacctgt
     1861 acgaaaactg cggcctgcgc gaccgcgaat cccatctggg ccggatcgtg cggttgcact
     1921 gggacgggcg gcacctgtcc gatcccgcgg tgctggtcac cgggctgcac ggcaacgcgc
     1981 atcagttgaa agtcattggc gggctgctgt gcctgctcga taccgccaac cagcggatcc
     2041 tgcgcttcac gctggacggc gcgccggtcg acaccaagac cccgctgccg ccggcaccgg
     2101 ccaacgatac cagcggggcc tatcaccaca tcaacagcat tgccgagatc ggcgggcggg
     2161 tggcgatcct gctccacaac ggcaaggctc ccagtccgcg caagagcgag ctggtctggc
     2221 tcgacgccga ctggcaggtg gccggccggg gcgagatcga cggctatatg tgccatgata
     2281 tcgtggccga cggtgacggc acgctgtggc actgcgcctc cgccagcggc gagctgacgg
     2341 gcagcaacgg cgagcggatc aagctgagcg acagcctgat gacccgcgcc ttgtcgttct
     2401 gccgcgatca tctgctggtc ggcctgtcca gctttggcga gcgccagatc cgtgacgggc
     2461 tgtccggcga ggtgctggtg ctggaccggg atcggcgggt ggtggcgcac catgatgtcc
     2521 cggccgggcc ggccgacgta gtggccctgc ggtgacggga ctggggccgg acctcgacgc
     2581 ggtcaagcgg atggtgaccg gctttgaccg gttgggtggg agcggcgatt tcgcgcggtt
     2641 cgtcccgctg agcctctacg acgccaaggc cgcggcctgg accgtggcgc gcaaggacct
     2701 gccgctgacg ctgctgacca aggcgcagct ggacctggtc gaggagctgc tggtcggcat
     2761 cggccagcgc ggtgtgccgg gcgattgcat cgaagcggga gtgtggcgcg gcggcacggt
     2821 gatcctgatg cgggccgtgc tcgatgcgca cggcatggcg gaccggcagg tgatcgccgc
     2881 ggattccttc gccgggatcc cgcccagcac gcggttcctg cacgatccgg tcgatctgtg
     2941 gccggatcgc tggattgcct cgctggaaga agtgcaggcg aacattgccg gcgccggcat
     3001 gggcgatccc cggatcgaat tcgtggaggg cctgttcgcg gacagccttc cgcggctggc
     3061 tggccgccag ctggcctttg cccggctcga ttccgattcg catgattcgg tgatggattc
     3121 gctgacctgg ctgtatccgc tgctgatgcc ggggggcgcg atcctgatcg acgattggca
     3181 cctgttcggc tgccggctgg cggtcgatcg ctatcgccat cagcacggca tcaccgatcc
     3241 gatccagacg gtggcccgga acggatactg gttcaaaagc cagccctggg ggcagccggc
     3301 accctgatag gggtccggct ttaccgccca aggcattgta ttgcatggac tatcggctgg
     3361 cgcgccagcc ggcagcgccg cttacgagcg ctggcaatcg atgctgggat tgccgccgat
     3421 gtcggcgccg cggcccggga aggcaaaggt ttcgcgaatg tcgagctcgc gaatttccgg
     3481 ctccatccag gtcttgcgct cggtcttctg gttcggctgg gtcattccca aaattccttt
     3541 taaatcaaaa agatcgttca aacccgatcc tgctgtgccg ttcgacccgt tattataggc
     3601 tcatgccagg atctttactg gtcgcgattc tgataccacg cggaaggagt tctgtgaagc
     3661 gcaagcattg gcccatattc ggggccacgc ttcaaacgtg tcccgagtat tttacgtggg
     3721 caatgactta atgccgtggt gatcctgact gggccgttgc ttgcaaagat atttggtgat
     3781 tacgggttgc caagccgcgc aaggccgggc tctctggcct tatcggtggc tgacggaact
     3841 accccatgcc cgctttggtg ggcattgggt ggccgttctg gccgcggcgc agcttacgag
     3901 cgctggcaat cgatgaaggc gttgccgccg atgtcagcgc cgcgccccgg aaaggcgaag
     3961 gtgtcgcgaa tttcgagctc gcggatcgtg ggctcgaccc aggtcttgcg ctcggtattc
     4021 tggtcaggct gagtcattac cgctattccc ttgtggagcg acccacggac tggcccgcgc
     4081 gagaacacgc gccgaccacg ggtcgactat catgccggca ggcgtctgtg aaccgggaat
     4141 accggccaag cggtccacgc atcctccgtc gtggcgggtg acggcgggat ttgatttgcc
     4201 gcgctcttcg cctagactgc cctcctatgt tggagattgt ccaagaaccg gtgcggtcca
     4261 tgaccctggc cgaggagcgc gcgctggccc ggcaagccta tgaacgcgcc ggctctgcct
     4321 tgtggcggct gcgcggggcg cagctggcaa cgcaggacga tgactatgcc gaagtggtca
     4381 ccctgctcga acaggcggat gacctggaat tttccgaacg gctgatgctg atgcgcgcct
     4441 atctggcaat ggaaacaccc gccgatacgg agcgggcgcg gttgaccgcg gaagccgcgg
     4501 ttgcggtggc ggacctgccg ctgcagcgcg ccgccgcgct ggcggaccgg gccaaggccg
     4561 agcggcgcct gtcgctggcg cgggcggcgc gtggctcgct ggaagccgcc ctggcggacg
     4621 atccgcacaa caaggatgcc tgcaaacggc tggccgcgct cgatctggaa gagggacgcg
     4681 ccccccaact ggtcgactgg tgcaaatcgc tgaccggttc gggcgttggc catgcccggc
     4741 ttcacgccgc gcacgtgctg ggcgaggcgg cccgcggcga tctggacgcc gcgcgcgcgg
     4801 tggagggctt tgccgcgctg agcgagagcg cgatcctgcc ccccccgccg ggctggagtg
     4861 atctggccgc gttcaacgcc gcgctggcgg ccgagctgct gggccaccgc aacatgcggt
     4921 acgaacgcta tggcagcgcg tccaactaca cctggcggat cgattggctg gcccgccccg
     4981 aaacgccgct ggtgcgagtg ctgctcgatc acctggccgg gcacatccag gcacgggttg
     5041 gccggcttga ccgggccgac caccgctggc tggccgacaa gcccgagcgg gcgctgctgc
     5101 gcgcctggtg cgtcatcacc gaaggcgacg ggttcgaagg ctggcacgtc caccagttcg
     5161 gctggctgag cggggtctac tatgtccaga tcccggagga gatcgcgctg ggccgcgatc
     5221 tgggcggctg cctggggttc ggtctgcccg atgacctgtc cggccaggcc gtgtcggatg
     5281 cctacgggac gacggtgatc cgcccggaac cgggcatgat ggtgacgttc ccctcgcaca
     5341 ccttccaccg cacctttgcc caccacgggc cgggcaagcg gatttgcatc gcgttcgatg
     5401 tgaagccggc ctgagcaccg gcattccccc cgcgatgcac ctgccggttc ctttcctgat
     5461 cggatcgcaa ggggtgaccg tgcagggcga tccggccccg tggcgcgacg cctttgcctc
     5521 cctgccggcg atcgccattc cggcggcgat cgagcccgcg ttctgcgcgc ggatggtgga
     5581 acgcgccgcg caggcccatt ttgccgagga cgacgttgcc tacatcggga cccgcgcgat
     5641 tgaagaaccg cagcgggtcg gccgggcgct atcgctgctg ctgcattcgc ccgagctgct
     5701 ccactggctt gaacaggcga cgggaacggg cacgcttcgg gcggttgccg gaaggctggt
     5761 cgaaacgcgg gccaaccacc gcgacgcgct cgactggcac gacgaccgcg acgatctcac
     5821 gcggcggctg gccatcgtca tcgacctttc ggaccagcgc tatcagggcg ggcaattcca
     5881 gctgcgccgc aagggcgagg ccgagccgct gttgagccac gatcacgctc aggccggatc
     5941 ggtgctgatc ttcgcggtgc ggcccgagct ggagcaccgg gtgaccccgg ttctcgcggg
     6001 cggcccccgc cgggtctttg cgggctggtt cctgacccag ccggagcacg ccagcggcgc
     6061 actcggcgcg tcaggctgag gccttcgccg ccatccgtgc gatagccgtc aacgcctgat
     6121 cgacccgccg cgcccgcgcc tcgggatcaa aggcccaggc ttccggtacc agcgccatcg
     6181 gcaccgtgcg ggccaggccc agaagacctt cgcgaattgc ggcacggccg ggcagggcgg
     6241 agaggatgcg ggggcggaac aggaccgagc cataggccat ggccttgtcc gccgcagttg
     6301 gcccgttgcc gatgatctgg cccagcagga tcagcccgcc gaccggccag ccccgatcgg
     6361 ccgcgcgggc gctgggccgg accagcacct tgccgcggcc gtcatcgatc ggttcgggcg
     6421 ggccttcgat tgccaggcca gcgcagctgg ccgggtggag ccgcatcgcc gggcggccgc
     6481 gccaggccag cggctggggg ctggggctca gcgcggtcag gtcatcggcc agaagctcgg
     6541 ccccggcggc cagcagttcg gcggtcaacg tggacttgcc cgcacccggg ctgccggcga
     6601 tcagccaggc cttgccgtgc aacaccacgg tggaggcgtg gagcggcagc agcccgcgcc
     6661 aggccagcgt gatcgccgcg accgagctga agaacgagac cggaagccgc ccgttccagt
     6721 ccgggcccgg gatccagccg attgcgccgc cgccgtgaat ttcgaagctg acctcgtcct
     6781 c
//
LOCUS       DASUDV010000220         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1950775,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000220 DASUDV010000000
VERSION     DASUDV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950775"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFV30_04725"
     CDS             complement(<1..407)
                     /locus_tag="VFV30_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEX4847424.1"
                     /translation="MCEFSAMAGPDPLLLDALLRRIGQWRAGHSGLFVLGLSGSQGSG
                     KTTLVAGLEQALEAQGLRVARLSLDDLYLTRAERIALGQSVHPLLATRGVPGTHDVAL
                     GLEVIAALERGKAAALPRFDKGRDDRADPATWP"
     gene            522..2021
                     /locus_tag="VFV30_04730"
     CDS             522..2021
                     /locus_tag="VFV30_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX4847425.1"
                     /translation="MRLTVPSLRGAFLIKAMLALIVVALGDFLFWQRGQWAGAQGLFG
                     LALASALVAARPAVIADRRALSAAALAAIFALAMIWDPSLLAWVLFWVAIGLATLLPA
                     TALFGDGWQWFQRLLVHAIKALFGPLIDFARLGRVRLRRGPSRVGLRSGMRTLVLPLL
                     GSAVILALFAAANPLIDGWLASLSLPRIDEQTFGRLLLGAVLFVMAWGVLRPRLLRRT
                     LGTFDGRGDLAIPGVNTASVLLSLIAFNALFALQNAMDLAYLSGWAQLPSGITLAQYA
                     HRGAYPLVATALLAALFVLVALRPGSATATMPLARRLVALWIGQNLLLVASAAWRTWD
                     YVEAYSLTELRIAALAWMALVAVGLVLVLWRIVKEHSASWLINANLAAAGGLLAAICF
                     VDLGGVAASWNARHAREIDGTGAGLDLCYLAGLEDSALPALVELEQRRLPGDLGRKIG
                     AIRWQVQTSLEGRIENGGWSLLAERRLAAARLVPPSNPSPERDCHVQHD"
BASE COUNT          276 a          745 c          781 g          387 t
ORIGIN      
        1 aagggccagg ttgcgggatc ggcgcgatcg tcgcgaccct tgtcgaagcg aggcagcgcc
       61 gccgccttac cccgttcgag cgcggcgatc acctccagcc ccagcgcgac atcgtgggtg
      121 ccgggcacgc cgcgcgttgc cagcagcggg tgcacgcttt gccccagcgc gatccgttcg
      181 gcgcgggtca ggtagagatc atcgagcgag agccgcgcga cccggagccc ttgtgcttcc
      241 agcgcctgtt ccagcccggc caccagcgtg gtcttgcccg agccttgact gcccgagagc
      301 cccagcacga acagcccgga atgccccgcg cgccactgcc cgatccggcg cagcagcgca
      361 tcgagcagca ggggatcggg gccggccatg gcgctgaatt cgcacatctt ggcccttctg
      421 cacaacatct gcaccgatcg cgcgcgaaga acccacgccg ccctgcgcat ggcctgcttg
      481 gcccggcgcc aaaggtgcct gcaaattccg ggagacatgc gatgcgattg accgttccga
      541 gcctgcgcgg ggcattcctg atcaaggcca tgctggcgct gatcgtggtg gcgctgggcg
      601 atttcctgtt ctggcagcgc gggcaatggg ccggcgccca gggcctgttc gggcttgccc
      661 ttgccagtgc gctggtggcg gcgcgccccg cggtgattgc cgaccggcgc gcactgagcg
      721 cggccgccct tgccgcgatc tttgccctgg cgatgatctg ggatcccagc ctgctggcct
      781 gggtgctgtt ctgggtggcg atcgggctgg ccacactgct gcccgcaacc gcgctgtttg
      841 gcgatggctg gcaatggttc cagcggctgc tggtccacgc catcaaggcg ctgttcggcc
      901 cactgatcga ctttgcccgg ctgggccggg ttcgcttgcg gcgtgggcct tcgcgggtcg
      961 gcctgcgcag cggcatgcgg acgctggttc tgccgctgct gggcagcgcg gtgatcctgg
     1021 cgctgtttgc cgcggccaat ccgctgatcg atggctggct ggcctcgctc agcctgccgc
     1081 ggatcgacga gcagaccttc ggccgcttgc tgctgggtgc agtgctgttc gtcatggcct
     1141 ggggggtgct gcgcccgcgg ctgctgcgcc gcacactggg caccttcgac gggcgcggcg
     1201 atctggcgat ccccggggtc aacaccgctt cggtgctgct ttcgctgatc gccttcaacg
     1261 cgctgttcgc gctgcagaac gcgatggacc ttgcttacct gtcgggttgg gcgcagctgc
     1321 ccagcggcat cacccttgcc caatatgccc atcgcggcgc ctatccgctg gtcgccaccg
     1381 cactgctcgc cgcgctgttc gtgctggtcg cgctgcgccc gggttcggcc accgcgacca
     1441 tgccgctggc ccgccgcctg gtggcgctgt ggatcggcca gaacctgctg ctggtggcca
     1501 gcgcggcctg gcgcacctgg gactatgtcg aggcctattc gctgaccgaa ctcaggatcg
     1561 ccgcgctggc ctggatggcg ctggtggcgg tggggctggt gctggtgctg tggcggatcg
     1621 tgaaggagca ttcggcgtcc tggctgatca atgccaacct ggccgcggcc ggggggctgc
     1681 tcgccgcgat ctgctttgtc gatctgggcg gggtggcggc cagctggaac gcgcgccatg
     1741 cccgcgagat cgacggcacc ggtgcggggc tcgatctgtg ctatctggcg gggctggaag
     1801 attccgcgct acccgcgctg gtcgagctgg aacagcgccg cctgcccggt gatctgggcc
     1861 gcaagatcgg cgcgatccgc tggcaggtgc agaccagcct tgaaggtcgg atcgagaacg
     1921 gcggctggtc gctgctggcc gaacgcaggt tggccgctgc ccggctggtg ccgcccagca
     1981 accccagccc ggagcgcgat tgccatgtcc agcatgattg acgctgcgcc acaaagcgcc
     2041 acaatggccg ccatgtcgcg cggcgtcctt atcgtcgatg atgatccgca catccgccag
     2101 gtgctggcct tcgcctttgc caaggccggg atggaggtaa gcgaagccgg cgacggcgag
     2161 gaggcgctgg ccggggtggc gcaggcccg
//
LOCUS       DASUDV010000221         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1089610,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000221 DASUDV010000000
VERSION     DASUDV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089610"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /gene="trmFO"
                     /locus_tag="VFV30_04735"
     CDS             complement(<1..1194)
                     /gene="trmFO"
                     /locus_tag="VFV30_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX4847426.1"
                     /translation="MTYDVHIIGGGMAGSEAAWQLARRGLKVRLSEMRGGGDTTPAHQ
                     GDGLAELVCSNSFRSDDHENNAVGLIHHEMRQLDSLIMGAAEVAKVPAGSALAVDRDV
                     FSAEVEKRLAALPSLEIVRERIDRLPEAGLTIVATGPLTAASLAQSIGAATGTESLAF
                     FDAIAPIVHHDSIDMSVAWMAARWDKGGKDYINCPMTREQYLAFHQGLLDGEKTEFRE
                     WEANTPYFEGCMPIEVMAARGVDTLRFGPMKPVGLDNPHWATEEHPNGRWPYAVVQLR
                     QDNKLGTLWNMVGFQTKLKYAEQVRLFRTIPGLENAEFARLGGLHRNTFLNSPVLLDR
                     QLRLKRAPHIRFAGQITGCEGYVESAAVGLIAGLRTAAELGGRPWQAPPRTTALGALL
                     SHITGD"
     gene            1263..1919
                     /locus_tag="VFV30_04740"
     CDS             1263..1919
                     /locus_tag="VFV30_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEX4847427.1"
                     /translation="MPERALIEKRPWLLASLLAGISFYFVRDMAIPGLYLMAWKGAGV
                     ALLAVYALARHQGADSRQIAAVMAFGALGDVLIELMLEAGAVAFLIGHLIAVHLYWRH
                     RRESLSFSQKLTGLALLVLVPLIAFQIPTDRTAAPGIALYSAGLGAMAAAAWTSSFPR
                     YRVGIGAVLFVISDLLIFARMGVLAQSPLPNLLIWPLYYFGQFLICTGVVGELRRRAA
                     "
     gene            1989..3764
                     /locus_tag="VFV30_04745"
     CDS             1989..3764
                     /locus_tag="VFV30_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847428.1"
                     /translation="MTLSRTLFAFLSALFVLSAPAVAKDKPPTHYDNSRESFVFDGRQ
                     NYPYVIRGVMIDWELACLKGKSDACLKLARAFETGFGVIPADARAAVGYFLEACKKGS
                     GEGCVRAAEMLREGTPGYKLPALAQQQAERGCTQLKHQPACASLAVSQAAAAPSGSGQ
                     SDALLAKACAAGDDTGCRNRANGLFYVRKDAAARAEALKLFEPACQARKSWGCMGMAD
                     AYRNGWGVAADPVRAADYERIGCVETRGDKLRLCTYHGERLSQSRNKADLNRGEQYLS
                     AACSANDGLACFQVARIGVQSLPGSTTTFQETMYFARRGCELGEGRACLMLGMIYAGD
                     FGKVQTETPVAFALFERGCKMGEQAACGREARLTGADPGLRTRRPAIDPAAPAVQQLQ
                     RAKALVEAGGPQRNDGLQTVARLMREGNEDAEWLLGGWMYFGLPGLFGSERKADGLVL
                     FENAARVGHVDAAIFMGMAYWYGRDVPLDRAKGQNYMAIAAARGDQKAAAILRSMQNE
                     PIREANERRQKQYEQWAAESRAFWNSPASMFSRSWSAPPTRYNPTPTYGGPSVSQIYD
                     NHNFNNAISYYSGGTSSCAASNPYC"
     gene            3779..>3970
                     /locus_tag="VFV30_04750"
     CDS             3779..>3970
                     /locus_tag="VFV30_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847429.1"
                     /translation="MRHLFVSLALLGGLAVPAAAQLNARFAQSENNGVCYFSVEGPGP
                     KGGSQSFTLEFSLRVSDSNL"
BASE COUNT          622 a         1337 c         1359 g          652 t
ORIGIN      
        1 atcgccggtg atgtgcgaca gcagcgcccc cagcgccgtg gtccgcggag gggcctgcca
       61 gggccgcccg cccagttcgg cggcggtcct caggcccgcg atcaggccca ccgcggcgct
      121 ttcgacatag ccctcgcagc cggtgatctg cccggcaaag cggatgtgcg gggcccgctt
      181 cagccgcagc tgccggtcca gcaggaccgg ggaattgagg aaggtgttgc ggtgcagccc
      241 gcccagccgg gcgaactctg cgttctccag ccccgggatg gtgcggaaca ggcggacctg
      301 ctcggcatac ttcagcttgg tctggaaacc gaccatgttc cacagcgtgc caagcttgtt
      361 gtcctggcgc agctgaacca cggcataggg ccagcgaccg ttcgggtgtt cttcggtcgc
      421 ccagtgcggg ttgtcgaggc caaccggctt catcggcccg aaccgcagcg tatccacccc
      481 ccgcgccgcc atcacctcga tcggcatgca gccttcgaaa tagggggtgt tggcctccca
      541 ttcgcgaaac tcggtcttct ccccgtcgag caggccctgg tggaaggcca ggtactgttc
      601 gcgggtcatc gggcagttga tgtaatcctt gccgcccttg tcccagcggg ccgccatcca
      661 ggcgacggac atgtcgatgc tgtcgtggtg gacgatcgga gcgatggcat cgaagaaggc
      721 caggctctcg gtgcccgtgg ccgcgccgat gctctgcgcc aacgaggcgg cggtgagcgg
      781 cccggttgcg acaatggtca ggccagcctc aggcagccgg tcgatccgct cgcgcacgat
      841 ctccagcgag gggagggcgg cgagccgctt ttccacctcg gccgaaaaga cgtcgcggtc
      901 cacggccaaa gccgaaccgg ccggaacctt ggccacttcc gccgcgccca tgatcagcga
      961 atcgagctgg cgcatctcgt ggtggatcag gccgaccgcg ttattctcgt gatcgtcgga
     1021 ccggaacgag ttggagcaga ccagctcggc cagtccatcc ccctgatggg ccggggtggt
     1081 atcgccgccg ccgcgcattt ccgaaagccg gaccttcagc ccgcgccggg cgagctgcca
     1141 ggcggcttcg cttccggcca tgccgccgcc gataatgtga acgtcatagg tcattgcccg
     1201 cccgcctagc cttgcgcggc gaagattcca acgccaagca ggaatgaccg gaggaggaaa
     1261 ccatgcccga gcgcgccctg attgagaaac gcccctggtt gttggcgagc ctgctggccg
     1321 ggatcagctt ctacttcgtg cgcgacatgg ccattcccgg gctctacctg atggcctgga
     1381 agggcgccgg ggtggcgctg ctcgcggtct atgccctggc ccgccaccag ggcgcggatt
     1441 cgcggcagat cgcggcggtc atggcctttg gtgcgctggg cgacgtgctg atcgagctga
     1501 tgctggaggc cggtgccgtg gcgttcctga tcggccacct gatcgcggtc cacctctact
     1561 ggcggcaccg gcgcgagagc cttagcttca gccagaagct gaccggcctg gcgctgctgg
     1621 tgctggtccc gctgattgcg ttccagatcc cgacggaccg caccgccgcg cccggcattg
     1681 cgctctattc cgccggcctt ggcgcgatgg cggcggccgc ctggaccagc agcttcccgc
     1741 gctaccgggt ggggatcggt gcagtgctgt tcgtcatctc cgaccttctg atcttcgcgc
     1801 ggatgggcgt gctggcgcaa agcccgctgc cgaacctgct gatctggccg ctctattact
     1861 tcggccagtt cctgatctgc accggggtgg tgggggaact gcggcgacgc gcggcgtagg
     1921 gtcttgccaa ggcggggcgt tccggcaatt ctgtccatgg gaagcgcgcc ccggatcgag
     1981 gtacctcggt gacattgtcg cgcaccctgt tcgctttcct gtcggccctg ttcgtgctgt
     2041 ccgccccggc cgtggccaag gacaagccgc caacccatta cgacaacagc cgcgaaagct
     2101 tcgtcttcga cgggcggcag aactatccct acgtgatccg cggggtgatg atcgattggg
     2161 aactggcctg cctgaagggc aagtctgatg cctgcctcaa gctcgcccgc gcctttgaaa
     2221 ccggattcgg ggtgatcccg gccgatgcgc gtgcggcggt cggctatttc ctggaggcct
     2281 gcaagaaggg ctccggcgaa ggctgcgtcc gcgcggccga gatgctgcgc gaaggcacgc
     2341 cgggctacaa gcttccggcg ctggcccagc aacaggccga gcgcggctgc acccagctga
     2401 aacaccagcc ggcctgcgct tcgctggcgg tgagccaggc cgccgctgcg cccagcggtt
     2461 ccggccagtc ggacgccctg ctggccaagg cctgcgccgc gggcgacgac accggatgcc
     2521 gcaaccgggc caacgggctg ttctacgtcc gcaaggacgc ggccgcccgg gccgaagcgc
     2581 tcaagctgtt cgaaccggcc tgccaggcgc gcaaatcgtg gggctgcatg ggcatggccg
     2641 atgcctaccg aaacgggtgg ggcgtggccg ccgatccggt gcgcgcggcg gactatgaac
     2701 ggatcggctg cgtcgagacc cgcggcgaca agctgcgtct gtgcacctat cacggcgaac
     2761 ggctgagcca gtcgcgcaac aaggccgatc tcaaccgcgg cgagcagtac ctgtccgcgg
     2821 cctgctcggc caacgacggg ctggcctgtt tccaggtcgc ccggatcggc gtgcagagcc
     2881 tgccggggtc aaccaccacc tttcaggaaa cgatgtattt cgcccggcgc ggctgcgagc
     2941 tgggggaggg gcgcgcatgc ctgatgctcg gcatgatcta cgccggtgat ttcggaaagg
     3001 tgcagaccga aaccccggtg gccttcgcgc tgttcgaacg gggctgcaag atgggcgagc
     3061 aggcggcgtg tgggcgcgag gcccggctga ccggagccga tcccggtctg cgcacccgcc
     3121 gccccgcgat cgaccctgcc gcgccagcgg tgcagcagct gcagcgggcg aaagcgctgg
     3181 tcgaggctgg cggaccgcag cggaacgacg gcctgcagac cgtggcccgg ctgatgcgcg
     3241 aagggaatga ggacgccgaa tggctgctgg gcggatggat gtacttcggc ctgcccggcc
     3301 tgttcggcag cgagcgcaag gccgatggac tggtcctgtt cgagaacgcc gcgcgggtcg
     3361 gccatgtcga cgccgcgatc ttcatgggca tggcctattg gtacgggcgc gatgtgccgc
     3421 tcgatcgcgc caaggggcag aactacatgg cgatcgccgc ggcgcgcggc gatcagaagg
     3481 cggcggcgat cctgcgatcg atgcagaacg agccgatccg cgaggcgaac gagcggcgcc
     3541 agaagcagta cgagcaatgg gccgccgaga gccgcgcctt ctggaacagt ccggcctcga
     3601 tgttctcacg cagctggtcc gccccgccga cccgctacaa ccccacgccc acctatggcg
     3661 ggccctcggt ttcgcagatc tacgacaacc acaacttcaa caacgcgatc agctattact
     3721 ccggcgggac cagctcctgc gccgcatcca acccctattg ctgaggagtt cccgcgccat
     3781 gcgtcacctg ttcgtttcgc ttgccctgct gggcggtctc gcggttccgg ccgcggccca
     3841 gctcaacgcc cgcttcgccc agtccgagaa taacggcgtg tgctatttca gtgtcgaagg
     3901 gccggggccg aagggcggat cgcagagctt cacgctggag ttcagcctgc gggtaagcga
     3961 cagcaacctg
//
LOCUS       DASUDV010000222         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_956873,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000222 DASUDV010000000
VERSION     DASUDV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956873"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="hutH"
                     /locus_tag="VFV30_04755"
     CDS             complement(<1..1259)
                     /gene="hutH"
                     /locus_tag="VFV30_04755"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX4847430.1"
                     /translation="MITLVPGNVSLAEWRAIHGGASVQLDPASAPRIAASAEAIARIL
                     ARPEPVYGINTGFGKLASVRIDDADLATLQRNIVLSHAAGTGAPSPVPVVRLMMALKL
                     ASLAQGASGVRPETVALLEAMLQRGLTPVVPCQGSVGASGDLAPLSHMAAAMIGVGEI
                     IVDGAALPAAQALAKAGFAPLELAAKEGLALLNGTQFSTANALAGLFGAENLFASALV
                     TGALATEAAKGSDTPFDPRIHTLRRHRGQIEVADALRQLMAGSAIRASHAVDDPRVQD
                     PYCLRCQPQVMGAALDVLRQAATTLEIEANGVSDNPLIFADTDEALSGGNFHAEPVAF
                     AADMIALAVCEIGSIAERRIAMLVDPALSGLPAFLTPRPGLNSGFMIPQVTAAALVSE
                     NKQRAYPASVDSIPTSANQEDHVSMAA"
     gene            complement(1256..2074)
                     /locus_tag="VFV30_04760"
     CDS             complement(1256..2074)
                     /locus_tag="VFV30_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX4847431.1"
                     /translation="MSDTELANTFARDGAVVIRGVLDAAAIGTLRAGIDANLANPSAN
                     AKIASGADDPGQFIEDFCCWQANAHYRELIWNSALPRLAALLTGSTQVRLYHDHMLTK
                     AAGTRQPTPWHQDQPYYNIDGRQTVSFWIPADQVLRESSLEFVAGSHLGPWLMPRTFM
                     DSEAKWFPEGSLAELPDIDADPARYPVLGWALEPGDVLAFNMLSLHRAAGSATRRRVL
                     SLRFVGDDVRHAPREWRTSPEFPGLAARLPAGAELNDPLFPLVWAGGQTCEMPR"
     gene            complement(2071..>2406)
                     /locus_tag="VFV30_04765"
     CDS             complement(2071..>2406)
                     /locus_tag="VFV30_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4847432.1"
                     /translation="FVRETKLPPVAALRAAGVPIALATDCNPGTSPLTSLLLTMNMAA
                     TLFRLTVNECLLGVTAHAARALGMAGQIGTLEAGKRCDLAIWDIERPAELVYRMGLNP
                     LHARVWGGR"
BASE COUNT          395 a          770 c          867 g          374 t
ORIGIN      
        1 tgtgcggcca tcgaaacgtg gtcttcctgg ttggccgatg tcgggatcga atcgaccgag
       61 gccggatagg cgcgttgctt gttttcgctg accagcgcgg cggcggttac ctgcgggatc
      121 atgaacccgg aattgagccc cgggcgcggg gtgaggaagg ccggcaggcc cgacagcgcc
      181 ggatcgacca gcatggcgat gcgccgttcg gcgatcgaac cgatttcgca caccgccagc
      241 gcgatcatgt cggcggcgaa ggctaccggt tcggcgtgga aattgccgcc cgaaagcgcc
      301 tcgtcggtat cggcgaagat cagcggattg tcggagacgc cgttggcctc gatctccagc
      361 gtggtcgccg cctggcgcag cacatccagc gccgcgccca tcacctgggg ctggcagcgc
      421 aggcaatagg gatcctgcac ccggggatcg tcaaccgcgt ggctggcgcg gatcgccgat
      481 ccggccatca attggcgcag cgcgtcggcc acttcgatct ggccgcggtg ccggcgcagg
      541 gtgtggatgc gcggatcgaa cggggtgtcc gatcccttgg cggcttcggt ggcgagcgcg
      601 ccggtaacca gtgcagaggc gaacaggttt tccgcgccaa acagcccggc aagcgcgttg
      661 gcggtggaaa actgcgtacc gttgagcagc gccagccctt ccttggccgc gagttcgagc
      721 ggggcgaagc cggccttggc cagcgcctgg gcagcgggca gggccgcgcc atcgacgatg
      781 atttcgccaa ccccgatcat cgccgcggcc atgtgcgaaa gcggggccag atcgccgctg
      841 gcgccgaccg aaccctggca cggcaccacc ggggtcagtc cgcgctgcag catggcctca
      901 agcagcgcga ccgtttctgg ccgcacgccc gatgcaccct gggcaaggct ggccagcttc
      961 agcgccatca tcagccgcac caccggcacc gggctgggtg cgccggttcc ggcggcgtgg
     1021 ctgagcacga tgttgcgttg cagggtggca agatctgcat catcgatccg caccgaggcg
     1081 agctttccga acccggtgtt gatcccatag accggttcgg gccgggccag gatccgggcg
     1141 atcgcttcgg cgctggcggc gatccgcggg gcagatgccg gatcaagctg cacgctggcg
     1201 ccgccgtgga ttgcgcgcca ttcggccagg gaaacgttgc cgggaaccag ggtaatcatc
     1261 ggggcatctc gcaggtttgt ccgccggccc agaccagcgg aaacaggggg tcgttcagtt
     1321 cggcgccggc cggcaggcgc gcggcaaggc cgggaaattc gggcgaggtg cgccattcgc
     1381 gcggggcatg gcgcacatca tcgccaacaa agcgcagcga cagcacccgg cggcgggtgg
     1441 cgctgccggc ggcgcggtgc aggctcagca tgttgaaggc cagcacatcg cccggctcca
     1501 aggcccagcc cagcaccgga tagcgcgccg gatcggcatc gatatcgggc agttcggcaa
     1561 ggctgccttc gggaaaccac ttcgcctcgc tatccatgaa ggtgcgcggc atcagccagg
     1621 ggccaaggtg cgacccggcc acgaattcaa ggctggattc gcgcagcacc tgatcggccg
     1681 ggatccagaa gctgaccgtc tggcgcccat cgatgttgta atagggctgg tcctggtgcc
     1741 aaggggttgg ctggcgggtg cctgcggcct tggtcagcat gtgatcgtga tagagccgta
     1801 cctgcgtgct gccggtcagc agcgcggcaa gccggggcag ggcgctgttc cagatcagtt
     1861 cgcggtagtg cgcattggcc tgccagcagc agaaatcctc gatgaactgg ccgggatcgt
     1921 cggcgcccga agcgatcttg gcattggcgc tgggattggc gagattggca tcgatcccgg
     1981 cgcgcagggt gccgatcgcg gctgcgtcca gcacgccgcg gatcaccacg gcgccatcgc
     2041 gggcgaaggt attggcaagc tcggtgtcgc tcatcgtccg ccccagacac gcgcatgaag
     2101 cgggttcagg cccatgcggt agaccagttc ggccgggcgc tcgatatccc agatcgccag
     2161 gtcgcagcgc ttgccggcct ccagcgtgcc gatctggccg gccatgccca gcgcccgcgc
     2221 ggcatgggcg gtgacgccca gcaggcattc gttgacggtc aggcggaaca gcgttgccgc
     2281 catgttcatc gtcagcagca gcgaggtgag cggcgaggtg ccgggattgc aatcggtggc
     2341 cagcgcaatc ggcaccccag cggcacgcag ggcggcgacg gggggcagtt tggtttcacg
     2401 tacgaa
//
LOCUS       DASUDV010000223        14065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_73370,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000223 DASUDV010000000
VERSION     DASUDV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73370"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VFV30_04770"
     CDS             <1..1189
                     /locus_tag="VFV30_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX4847433.1"
                     /translation="VVSYDPALAERLGGVAIAEDQQRATLRALGFASDARWNVIVPSW
                     RPDVDGAADLVEEVVRIHGLDNVASAPLPRTDGVARPTATPVQQLERKVRRAAAARGN
                     HEAVTWSFLPEADAAAFAEGNGAVWTLANPISEDMKAMRPSLLPGLLAAVRRNADRGA
                     AGLRLFELGRRYLRGEGGHSDERLTLGLVLAGEKTPRGWSSGKAQSFDAFDAKAEALA
                     LLAEAGAPVENLQVMGEAGAQFHPGQSATLRLGPKTVLARFGMLHPALLKQSDIGLPV
                     AAVELFLDAIPAKKASGFARTAYAPPALQAVKRDFAFLVDAALPAGDLVRTVKGADKA
                     NIVAVRLFDDFRGQGVPDGQKSLAVEVTLQPGEKSFTEADLKAVAEKVTAAAAKLGAV
                     LRG"
     gene            1186..1638
                     /locus_tag="VFV30_04775"
     CDS             1186..1638
                     /locus_tag="VFV30_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847434.1"
                     /translation="MSGRVRSALLGAGLLLLAGAAEPAGEYQTGGRWRVTVTPVQMPA
                     AAGEQKGTDKTTCRATLTSQSYPKVGREKELAPGVICKIVSITPLGQPYERVDECRRA
                     GQSKAMIVRYSGSYTPKRYSLTITTEMPSRPDLTVVVREDGEWLGPCE"
     gene            1713..2054
                     /locus_tag="VFV30_04780"
     CDS             1713..2054
                     /locus_tag="VFV30_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847435.1"
                     /translation="MDRSARKWVIGLSALLTFVSLVALLGAVSSDTTLPALAIGLTAW
                     ALSLMRRLVALTLFGPPLAFLLLLGANSEGLTRGVVLYAAIALGIALAAAVGGGWVFD
                     RFLLKTPGAKG"
     gene            complement(2051..2350)
                     /locus_tag="VFV30_04785"
     CDS             complement(2051..2350)
                     /locus_tag="VFV30_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847436.1"
                     /translation="MGIAVYGFAILAFIGALWWFNHWRQTRLDADPGQQRLAELLVSA
                     ALGRGASEQQVRDQLAAISRGGADARVRLTHAVMLVRSEAAPELYEKVLALSRQI"
     gene            complement(2352..3419)
                     /gene="aroC"
                     /locus_tag="VFV30_04790"
     CDS             complement(2352..3419)
                     /gene="aroC"
                     /locus_tag="VFV30_04790"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX4847437.1"
                     /translation="MSINTFGRLLRFTTWGESHGPALGAVLDGCPPGLALDESWIQPF
                     LDARRPGQSKFTTQRQEPDAVRILSGVFEGQTTGTPISLMIENVDQRSKDYSEVAKAY
                     RPGHADYAYDAKYGLRDYRGGGRSSARETAARVAAGAVARLVIPEVTVLGYVSEIGGD
                     AIDPANFDAGEIARNPFFCPDAAAAKRWEGLVDEARLSGSSLGAVVDCVATGVPAGWG
                     APLYGKLDADLAAAMMGINAVKGVEIGDGFAAARLTGEGNADPMRPGAEGPEFLANHA
                     GGIAGGISTGQPVVVRVAFKPTSSILTPVETIGRDGEATEIRTKGRHDPCVGIRGTPV
                     VEAMMALVLADHKLLHRGQCG"
     gene            3479..3790
                     /locus_tag="VFV30_04795"
     CDS             3479..3790
                     /locus_tag="VFV30_04795"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX4847438.1"
                     /translation="MYLTVFRNRKRPDMDRAAYEADNARMEELAASQPGFIAIKGFVA
                     EDGEVVAISEWESEEAAKAWGSHPEHARVQARGRADYYESYTLYSCTDPKVRRYERAR
                     P"
     gene            3787..4134
                     /locus_tag="VFV30_04800"
     CDS             3787..4134
                     /locus_tag="VFV30_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEX4847439.1"
                     /translation="MTVTVYGIPNCDTVKKARTWLDARGIAYAFHDYKKAGADPARLA
                     EWCMVAGWETVLNRAGTTFRKLPDEAKADLDPARAVALMAANPSCIKRPIVDYGDGLL
                     VGFKEAEWAAALG"
     gene            4113..4751
                     /gene="pnuC"
                     /locus_tag="VFV30_04805"
     CDS             4113..4751
                     /gene="pnuC"
                     /locus_tag="VFV30_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEX4847440.1"
                     /translation="MGGSPGMTFDPATLKTLEWIAAGLGVINIALLIFRSQWNFAFAI
                     ASVALFVVIFYESRLYAESGLQLFFILANVWGWLLWRKAARTGIDEGSRVPVRWLDWK
                     SRLVWLTVTAAISLNLGWLMHRYTNAAMPFADSAIAGASVAAQILLGFRRIENWIVWI
                     AVDVAAVMLYINRGLYPTAGLYGGMLVMCIFGLREWIEVERRQRAASPGPEA"
     gene            4748..5266
                     /locus_tag="VFV30_04810"
     CDS             4748..5266
                     /locus_tag="VFV30_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847441.1"
                     /translation="MIRICFHGAESTGKSVLAEKLAADLGLPLVAEYGREYAEARGTV
                     FAMDDLLAIAEGHEARVQAVEASGAPVCLLDTDPLMTAAWAAMLFGEVPDALLAYPKA
                     DLYLLFEADVPWVEDGTRLFGTSPERTRFAATAEAMLERAGVRYERVSGSWGEREAAV
                     RAAVQRAVTVTM"
     gene            complement(5239..5985)
                     /gene="ubiG"
                     /locus_tag="VFV30_04815"
     CDS             complement(5239..5985)
                     /gene="ubiG"
                     /locus_tag="VFV30_04815"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX4847442.1"
                     /translation="MANASSSSPPAATIRPEEAAHFGKLAAEWWDPKGSSAMLHRLNP
                     VRLGFVRAAIDAHWGTDSRGVRPLTGKTALDVGCGAGLLCEPLARLGAQVTGVDAAQE
                     NVAAASAHAAGAGLSIAYRCGDVAELGLTGFDLVTSMEVIEHVADKAAFLSALAGALA
                     PGGLMILSTPNRTAKSRLLMIEAAERIGMVPRGTHHWDDFVTPDELADLLTEAGLTMG
                     TPKGIAWSPTKGLHLSDDLALNYIVTVTAR"
     gene            6063..7331
                     /locus_tag="VFV30_04820"
     CDS             6063..7331
                     /locus_tag="VFV30_04820"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX4847443.1"
                     /translation="MARIVMKFGGTSMAGSERIRRVANIVRRQQAAGHEVAVVVSAMA
                     GETDRLVNFCREANPLYDPAEYDVVVASGEQVTSGLLAMTLQALGCKARSWLGWQLPV
                     RTDDAHAKARIEGIDSEALLASMAAGEIAVIPGFQGLTEGGRVTTLGRGGSDTSAVAV
                     AAAIGADRCDIYTDVDGVYTTDPRIVAKARKLKYVTYEEMLELASVGSKVLQTRSVSL
                     AMKEGVRVQVLSSFIDEDSPPADDIPGTMIVSDDELAKIQEGLDMERQLITGIAADKN
                     EAKVILTRVPDRPGAVATIFGPLAAANINVDMIIQNVGRDKGETDVTFTVPAADLLRA
                     QTLLEAQKQAIGYNRIITDGKVAKVSVVGVGMRSHAGVASTMFRALADRGINIQAIST
                     SEIKISVLIDEDETELAVRVLHTAYGMDAE"
     gene            7643..8932
                     /locus_tag="VFV30_04825"
     CDS             7643..8932
                     /locus_tag="VFV30_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4847444.1"
                     /translation="MPDDPPLPAFRFQPHPPSLWQLFRQGIGDVGSCIPEAILRDFAV
                     QLPGPGAPLVLSHPDLAREVLNDRAGQFERDKFIRRLFRRSWGEGLAGAEGPHWERQR
                     KASAPFFTPGSVKRQLQAFARETDGAMRALPDEDAVELNALALRIVARIVFSVLVEGR
                     GAIDPEAVARDVPGYIARVVDFGPADLLPLPERLLDWLNGVNRDPQVRRVRAAAQRLA
                     QERGNGPPRSDLIALLEGIGPVGDNIGGLIPAALDTTVHGLSWALNTLALRPEWQSRL
                     AEEARAAGPSPALEQLPLTRRVVQEVLRLYAPAPLLARSAGVDQVLAGHSVRRGQTVI
                     IAVYAMHRHPRLWDDPDRFDPDRFLPEHGSNPGWMPFGTGPRMCIAAQFAQAEMAVML
                     ARILAQFALHPSGHQPDMMLRTATRSRNGLVVRLQRR"
     gene            complement(8948..9316)
                     /locus_tag="VFV30_04830"
     CDS             complement(8948..9316)
                     /locus_tag="VFV30_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847445.1"
                     /translation="MAYDVYQSECPARAVLDHVGDKWALLVLHRLSEGPVRFNALRRD
                     IHGLSQKVLSQVLKRLERDGLVSRTVIATVPVTVEYALTGLGECLTANVGPLLRWAEA
                     NMEAIKAAQTAYDARSLEAA"
     gene            9453..10007
                     /locus_tag="VFV30_04835"
     CDS             9453..10007
                     /locus_tag="VFV30_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX4847446.1"
                     /translation="MAQTVVIYHSGYGHTKVVAEAVAAGAQGQLIAISPEGDISEDDW
                     AALDAADAIVFGAPTYMGSASWQFKKFSDATSKRWFTRDWADKVFGGFTNSASPSGDK
                     QVTLIQMQTLASQHGGIWVSLGLAPSNHKAATAADVNNLGGSVGLLVRSPSDASVDEV
                     HSGDLETARLYGTRVAGIAARLKG"
     gene            complement(10014..10568)
                     /locus_tag="VFV30_04840"
     CDS             complement(10014..10568)
                     /locus_tag="VFV30_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX4847447.1"
                     /translation="MTATTAASPIRAARRPAVPYAAYEPGPLVRAAVGLVGVGFTAAC
                     AIGLVRFAAGLAPAHAAPRDLAVMLHLATVLPAVPLGAWLMLSRKGTARHKLWGKVWV
                     VLMILTALSALFIRQINGGQLSPIHIFVPMTLWGAWKTVATARRGDIAGHRKQLVGFY
                     LGALTIPGLFAFLPGRMMWTWLVG"
     gene            10727..11533
                     /locus_tag="VFV30_04845"
     CDS             10727..11533
                     /locus_tag="VFV30_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX4847448.1"
                     /translation="MSQAAPPPSPHPIRQIVLDLGVMTVIGILLALLGPFGSFELPFA
                     YRLVYWLGLAWAGYACYRPIGGLITRLGPVLDLPDWSLWLIGCLVATAPMTVVVWLVG
                     ALPPPLELPSLEAWIVTYGYVLAIGAVVTTVFYLVQSKPAAAPEPIAVAAQPDPLPAA
                     PAAPPRARLLDRLPPRLGDEIVALEMEDHYLRVHTRLGSDLILLRLRDALAELDGLDG
                     AQVHRSWWVARGGVERVVRDGRNVALLLHGGLEAPVARSQVAVLKAAGWL"
     gene            complement(11595..13754)
                     /gene="katG"
                     /locus_tag="VFV30_04850"
     CDS             complement(11595..13754)
                     /gene="katG"
                     /locus_tag="VFV30_04850"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX4847449.1"
                     /translation="MNASTKCPVMHGGSTRADSSPTAWWPNALNLDILHQHDAKTNPL
                     GPDFDYREAVKGLDYDALKADLTALMTDSQDWWPADWGHYGGLMIRLAWHAAGTYRIA
                     DGRGGASTGNQRFAPLNSWPDNGNLDKGRRLLWPIKKKYGNALSWADLFILAGNVAYE
                     SMGFKTFGFGFGREDIWHPEKDIYWGAEKEWTAPTGSEGTRWSGDRELENPLAAVMMG
                     LIYVNPEGVDGQPDPLKTARDMRITFSRMAMDDEETVALTAGGHTVGKAHGNGDAGVL
                     GPAPEGGEIEDQGFGWLNKTGRGVGRDTVTSGVEGAWTSNPTVFDNGYFDMLFKHDWE
                     LTKSPAGANQYRPVNIATDDMPVDVEDPAIRTMPVMTDADMALKVDPEYRKISERFRD
                     DHAYFCEVFARAWFKLTHRDMGPKANYLGPWVPAEDLIWQDPVPAGSSTYDVGALKAK
                     IAASGLSAADLIATAWDSARTFRGSDKRGGANGARIRLAPQKDWAGNEPERLARVLAV
                     LEPLAAQAGASLADTIVLGGNVGLERAIEAGGHRVPVPFAPGRGDASAEQTDVDSFAV
                     LEPLADGFRNWLKGDYAVQPEELLLDRAQLMGLTAAEMTVLVGGLRALGVNHGGSAHG
                     VFTDRPGALTADFFVTLTDMAYRWVPKGRNLYELVERKTGKVKHTATRVDLVFGSNSI
                     LRGYAELYAQDDNAGKFVRDFVAAWTKVMNADRFDLN"
BASE COUNT         2164 a         4799 c         4851 g         2251 t
ORIGIN      
        1 ggtggtttcc tatgaccccg ccctggctga gcggctgggc ggcgtggcga ttgccgagga
       61 ccagcagcgc gccaccctgc gcgcgctggg ctttgccagc gacgcccgct ggaacgtgat
      121 cgtgccgagc tggcgcccgg atgtggacgg cgcggccgac ctggtcgagg aagtggtccg
      181 catccacggg ctggacaacg tggccagcgc ccccctgccc cgcaccgatg gcgtggcccg
      241 gccgactgcc accccggtcc agcagctgga gcgcaaggtc cgccgcgccg ctgcggcgcg
      301 cggcaaccac gaagcggtga cctggtcgtt cctgcccgag gccgatgccg cggcctttgc
      361 cgagggcaac ggcgcggtct ggaccctggc caacccgatc agcgaggaca tgaaggcgat
      421 gcgcccctcg ctgctgccgg gcctgctggc cgcggtgcgc cgcaacgccg atcgcggcgc
      481 ggcgggtctg cggctgttcg aactcggccg ccgttacctg cggggcgaag gcggccacag
      541 cgacgagcgc ctgacgctgg gcctggtcct cgccggcgag aagaccccgc gcggctggtc
      601 cagcggcaag gcgcagagct ttgacgcctt cgacgccaag gccgaagccc tggccctgct
      661 ggccgaggcc ggtgcgccgg ttgagaacct gcaggtgatg ggagaggcgg gcgcgcagtt
      721 ccaccccggc cagtcggcca cgctgcggct ggggcccaag accgtgctgg cccgctttgg
      781 catgctccac ccggcgctgc tcaagcagtc cgacatcggc ctgccggtgg cggcggtgga
      841 gctgttcctc gatgcgattc cggccaagaa ggcgtccggc tttgcccgca ccgcctatgc
      901 cccgccggcg ctgcaggcgg taaagcgcga ctttgccttc ctggtcgatg ccgcgctgcc
      961 ggcgggcgat ctggtccgca ccgtcaaggg tgcggacaag gccaacatcg tcgcggtccg
     1021 cctgttcgac gatttccggg gccagggcgt gccggacggg cagaagagcc tggcagtcga
     1081 agtgaccctg cagccgggcg agaagagctt caccgaggcc gacctcaagg ccgtggccga
     1141 gaaggtcacc gcggcggccg ccaagctggg cgcagtgctg cgcggatgag cgggcgggtg
     1201 cgatcggccc tgctgggcgc gggtctgctc ctgctggcgg gcgcggccga accggccggc
     1261 gagtaccaga ccggcggccg ctggcgggtt acggtcacgc cggtgcagat gccggccgcg
     1321 gcgggcgagc agaagggcac cgacaagacc acgtgccggg caaccctcac gtcgcaatcc
     1381 taccccaagg tcggccgcga gaaggagctg gccccggggg tgatctgcaa gatcgtctca
     1441 atcaccccgc tgggccaacc ctacgaacgg gtcgacgaat gccgtcgcgc gggccagagc
     1501 aaggcgatga tcgtgcgcta ttccggatcg tacactccga agcgttacag cctcaccatc
     1561 accaccgaaa tgccatcccg cccggatctg accgtggtgg tgcgcgaaga cggtgaatgg
     1621 ctggggccct gcgaataggc cgcgcaattt cgcgcgagat cgaccgcatt gtctgctaaa
     1681 agtccggacc ggcaccgcgg ggagtacgcc caatggacag gtcagcgcgc aaatgggtga
     1741 tcggcttgtc cgcactcctc accttcgttt cgcttgtcgc gctgctcggc gcggtcagca
     1801 gcgacacgac gcttcccgcc ttggcgatcg gccttaccgc ctgggcgctg tcgctgatgc
     1861 ggaggctcgt cgcgctgacc ctgttcggac cgccgttggc gttcttgctg ctgctgggtg
     1921 ccaacagcga agggctgacc cgcggcgtgg tcctttacgc agcgatagct cttggcatcg
     1981 ccctggccgc ggccgttggc ggcggctggg tctttgaccg cttcctgctc aagacgcccg
     2041 gcgccaaggg ctagatctgg cgcgagagcg cgagcacctt ttcgtaaagt tccggcgcgg
     2101 cttcgctgcg caccagcatc accgcgtggg tcaggcgcac ccgcgcgtcg gccccgccgc
     2161 gcgaaatcgc ggccagctga tcgcgcacct gctgttcgct ggccccgcgg cccagcgcgg
     2221 cgctgaccag cagttcggcc agccgctgct gcccgggatc ggcgtccagc cgcgtctggc
     2281 gccagtggtt gaaccaccac agcgcgccga tgaacgccag gatcgcaaac ccgtagacgg
     2341 cgatgcccat gtcagccgca ctgcccccgg tgcagcagct tgtgatcggc caggaccagc
     2401 gccatcatcg cttccaccac cggcgttccg cgaatgccga cgcagggatc atggcggccc
     2461 ttggtgcgaa tctcggtcgc ctctccgtcg cggccgatcg tttcgacagg cgtgaggatc
     2521 gaactggtcg gcttgaaggc cacgcgcacc accaccggct ggccggtgct gatcccgccg
     2581 gcaatcccgc cggcgtggtt ggccaggaat tcgggcccct cggcgccggg ccgcatcgga
     2641 tcggcgttgc cctcaccggt cagccgggcg gcggcaaagc catcgccgat ctccacgccc
     2701 ttgaccgcgt tgatccccat catcgccgcg gcaagatcgg cgtcgagctt gccatagagc
     2761 ggcgcgcccc agccggccgg aaccccggtg gcaacgcaat ccaccaccgc gccgagcgag
     2821 gatccgctga gccgggcttc gtccaccagc ccttcccacc gcttggccgc tgccgcatcg
     2881 gggcagaaga acgggttgcg ggcaatctcg cctgcatcga agttggccgg atcgatggcg
     2941 tcgccgccga tttccgagac atagcccagc accgtcactt cggggatcac cagccgcgcc
     3001 acggcgccgg cggccacccg cgccgccgtc tcccgcgcgc tggaacgccc gccgccgcgg
     3061 taatcgcgca ggccgtactt ggcgtcatag gcatagtcgg catggcccgg ccggtaggcc
     3121 ttggccactt cggaatagtc cttggaccgc tggtcgacat tctcgatcat gaggctgatc
     3181 ggggtaccgg tggtctggcc ctcgaacacg cccgaaagga tccgcaccgc gtccggttcc
     3241 tgccgctggg tggtgaattt cgactggccc gggcggcgcg catcgaggaa cggctggatc
     3301 cagctttcgt cgagcgcaag gcccggcggg catccatcga gcacggcgcc cagcgccgga
     3361 ccgtggcttt cgccccaggt ggtgaaacgc agcagacggc cgaaggtgtt gatggacatg
     3421 gatcacggct ttggcgcgtg attgcgccgc tgtccatatc cggtttagga aggccgccat
     3481 gtacctgacc gtgttccgca accgcaaacg gcccgacatg gaccgcgccg cctatgaagc
     3541 ggacaacgcg cggatggagg aactggcggc cagccagccc ggcttcatcg cgatcaaggg
     3601 cttcgtggcc gaggacggcg aagtcgtcgc catctccgaa tgggagagcg aggaagcggc
     3661 caaggcctgg ggcagccacc ccgaacacgc ccgggtccag gcccgcggcc gcgcggatta
     3721 ttacgagagc tacacgctct attcctgcac cgatcccaag gtgcgccgct atgaaagggc
     3781 ccgtccgtga ccgtgaccgt ctatggcatt cccaactgcg atacggtgaa gaaggcccgc
     3841 acctggctgg acgcgcgcgg cattgcctat gcctttcacg attacaagaa ggccggggcc
     3901 gatcccgccc ggctggcgga atggtgcatg gtggccggct gggaaaccgt gctgaaccgc
     3961 gccggcacca cctttcgcaa gctgccggat gaggccaagg ccgatctcga tccggcccgc
     4021 gccgtggcgc tgatggcggc caaccccagc tgcatcaagc ggccgatcgt ggactatggc
     4081 gatgggctgc tggtcggctt caaggaagcc gaatgggcgg cagccctggg atgaccttcg
     4141 atccggcaac cctgaaaacg ctggaatgga tcgccgccgg gctgggcgtg atcaacattg
     4201 ccctgctgat cttccgctcg cagtggaact ttgccttcgc gatcgcctcg gtcgcgctgt
     4261 tcgtggtgat cttttatgaa agccggctct atgctgaaag cgggctgcag ctgttcttca
     4321 tcctcgccaa cgtctggggc tggctgctgt ggcgcaaggc ggcgcggacc gggatcgacg
     4381 agggcagccg ggtgccggtg cgctggctgg actggaagag ccggctggtc tggctgaccg
     4441 tgaccgcggc gatcagcctc aacctcggct ggctgatgca ccgctatacc aacgccgcca
     4501 tgccgtttgc cgacagcgcg atcgccgggg ccagcgtggc cgcgcagatc ctgctgggct
     4561 ttcgccggat cgagaactgg attgtctgga tcgcggtcga tgtcgcggcg gtgatgctct
     4621 acatcaaccg cgggctctat cccaccgccg ggctctatgg cgggatgctg gtgatgtgca
     4681 tcttcggcct gcgtgaatgg atcgaggtgg agcgccgcca gcgcgcggca tcgcccggtc
     4741 cggaggcatg atccgaatct gctttcacgg cgcggaaagc accggcaagt cggtgctagc
     4801 cgagaagctg gcggcagacc tcggcctgcc gctggtggcg gagtacgggc gcgaatacgc
     4861 cgaagcccgc ggcaccgtct ttgccatgga cgatctgctg gcgattgccg aaggccacga
     4921 ggcgcgggtc caggccgtgg aagccagcgg cgcgccggtc tgcctgctgg ataccgaccc
     4981 gctgatgacc gccgcctggg ccgcgatgct gttcggcgag gtgccggacg cactgctggc
     5041 ctaccccaag gccgatctct acctgctgtt cgaagccgac gtgccctggg tggaagacgg
     5101 cacccgcctg tttggcacct cccccgaacg cacccgcttt gctgcgacgg cggaagcgat
     5161 gctggagcgc gcgggggtgc gctacgagcg ggtttccggc agctggggcg agcgtgaggc
     5221 agcggtgcgc gccgcggttc agcgggccgt caccgtcacg atgtagttca gcgccagatc
     5281 gtccgacagg tgcaggccct tggtggggga ccaggcgatg cccttgggcg tgcccatcgt
     5341 caggccggct tcggtcagca gatcggccag ttcgtccggc gtcacgaaat cgtcccagtg
     5401 gtgggtgccg cgcgggacca tgccgatccg ttcggccgcc tcgatcatca gcaggcgcga
     5461 cttcgcggtg cggttggggg tggagaggat catcagcccg cccggcgcca gcgccccggc
     5521 cagcgcggag aggaacgcgg ccttgtcggc cacgtgctcg atcacttcca tcgaggtgac
     5581 aaggtcaaag cccgtgaggc ccagttcggc cacgtcgccg cagcggtagg cgatggagag
     5641 gccggcgccg gcggcatggg cgctggccgc ggcaacgttt tcttgggccg catcaacccc
     5701 ggttacctgc gcgcccaatc gggccagtgg ctcgcacagt aggcctgcgc cgcagccgac
     5761 atcgagcgcg gtcttgcccg tcagcgggcg aaccccgcgc gaatccgtgc cccaatgggc
     5821 atcgatcgcc gcgcggacaa agcccagtcg caccgggttg agccggtgca gcatggccga
     5881 cgagcccttg ggatcccacc attcggccgc cagcttgccg aaatgggcgg cctcctcggg
     5941 ccggatcgtg gcggcggggg gactcgatga acttgcatta gccatgccgc ctatctaaca
     6001 gggcgcccgc tgcagggcga ggggtcctgt cggcaacaat gttgcgaaaa gggtgggaac
     6061 gcgtggcccg catcgtgatg aaattcggcg gcacctcgat ggcgggatcg gaacggatcc
     6121 gccgggtcgc caatatcgtg cggcgccagc aggcggccgg gcacgaagtg gccgtggtcg
     6181 tttccgccat ggccggcgag accgaccgcc tggtcaactt ctgccgcgag gcgaatccgc
     6241 tctacgatcc ggccgaatac gacgtcgtcg tggccagcgg tgagcaggtc acgtcgggcc
     6301 tgctggcgat gacgctgcag gcgctgggct gcaaggcgcg ctcgtggctg ggctggcaat
     6361 tgccggtgcg gaccgacgac gcccatgcca aggcccggat cgaaggaatc gattccgaag
     6421 ccctgctggc ctcgatggcc gcgggcgaga ttgcggtgat cccgggcttt cagggcctga
     6481 ccgaaggcgg ccgggtgacc accctgggcc ggggcggatc ggatacctcg gccgtggccg
     6541 tggccgccgc gatcggcgcg gaccgctgcg acatctacac cgatgtcgac ggggtctaca
     6601 ccaccgaccc gcggatcgtg gccaaggccc gcaagctgaa atacgtgacc tacgaggaaa
     6661 tgctcgagtt ggcctcggtc ggctccaagg tcttgcagac ccgctcggtc agcctggcga
     6721 tgaaggaagg ggtgcgcgtg caggtgctct ccagcttcat cgacgaggac agcccgccgg
     6781 ccgacgacat tccgggcacg atgattgtca gcgacgacga acttgccaaa attcaagagg
     6841 gattggacat ggaacgccag ctcatcaccg gcattgccgc cgacaagaac gaagccaagg
     6901 tgatcctgac ccgcgtgccc gaccggcccg gcgcggtcgc caccatcttc ggcccgctgg
     6961 ccgcggccaa catcaacgtc gacatgatca tccagaacgt cgggcgcgac aagggtgaga
     7021 ccgacgtgac cttcaccgtg ccggcggccg acctgctgcg cgcgcagacc ctgctggaag
     7081 cgcagaagca ggcgatcggc tacaaccgga tcatcaccga cggcaaggtg gccaaggttt
     7141 cggtggtcgg cgtcggcatg cgcagccacg ccggggttgc cagcaccatg ttccgcgccc
     7201 tggccgaccg cgggatcaac atccaggcga tctccaccag cgagatcaag atctcggtgc
     7261 tgatcgacga ggacgagacc gaactggccg tgcgcgtgct gcacaccgcc tatggcatgg
     7321 acgccgagta agccgcccga gcgtaacgct gaggttgaca cagttgacac tgcgtccgct
     7381 tcgtccaaca cgcgacgggg cacgggtttc tgccggttcc aagccggttc caggcgattg
     7441 gcgggtgcgg cgacaccaaa aaacggtgca ggctgcccgg acgggcgtgc cggaacggac
     7501 cggtttgcgg cgggcgatcg attgaccatc ccgcgataat cgcacaacca tgcggctgta
     7561 ggacagcgcg cggaacctgt cggccgcgat ggcggcggcg cacgcttcgg gctcgtttcc
     7621 ctttcctgcg ggaggactgg caatgcctga cgatccgccg ctgcccgcct ttcggttcca
     7681 gccgcatccg ccatcgctgt ggcagctgtt ccggcagggg atcggcgatg tcggcagctg
     7741 catccccgag gcgatcctcc gcgatttcgc ggtgcagttg ccgggccccg gcgcgccgct
     7801 ggtgctgtcc caccccgatc tggcccgcga ggtgctgaac gaccgggccg gccagtttga
     7861 gcgcgacaaa tttatccgcc gcctgttccg ccggtcctgg ggcgaggggc tggccggtgc
     7921 cgaagggccg cactgggaac gccagcgcaa ggcgtcggcg ccgttcttca cgccgggttc
     7981 ggtcaagcgg cagttgcagg cctttgcgcg cgagaccgac ggcgcgatgc gcgccctgcc
     8041 ggacgaggac gcggtcgagc tcaacgccct ggccctgcgg attgtcgcgc ggatcgtctt
     8101 ttcggtgctg gtcgaggggc gcggcgcgat cgaccccgaa gccgtcgccc gggatgtgcc
     8161 gggatacatc gcgcgggtgg tcgatttcgg tccggccgat ctgctgcccc tgccggagcg
     8221 cctgctcgac tggctgaacg gggtgaacag ggatccgcag gtgcgccgcg tgcgggcggc
     8281 ggcgcagcgg cttgcgcaag agcgcggaaa cggcccgccc cggtccgatc tgattgccct
     8341 gctcgaaggg atcggcccag tgggcgacaa catcggcggg ctgatccccg cagcgctcga
     8401 caccaccgtc catggcctca gctgggcgct gaacaccctg gcgctgcggc cagaatggca
     8461 gagccgcctg gccgaggaag cccgggcagc cggcccatcg cccgccctgg agcaactgcc
     8521 gctgacgcgg cgcgtggtgc aggaagtgct gcgcctctat gcgcccgccc cgctgctggc
     8581 ccgcagcgcg ggcgtcgatc aggttctggc cggccacagc gtccggcgcg gccagaccgt
     8641 gatcatcgcg gtctatgcca tgcaccgcca tccccggctg tgggacgatc ccgaccggtt
     8701 cgacccggac cgctttctgc cggaacatgg cagcaatcca gggtggatgc cgttcggaac
     8761 cggtccccga atgtgcatcg cggcgcagtt cgcccaggcc gagatggccg tgatgctggc
     8821 gcggatcctg gcgcagttcg cgctccaccc cagcggtcac cagccggaca tgatgctgcg
     8881 caccgccacg cgatcgcgca acggactggt ggtccggctg cagcgacgct gatccgcgtc
     8941 agcgcgctca ggccgcttcg aggctccgcg cgtcgtaggc cgtctgggcg gccttgatgg
     9001 cttccatgtt ggcttcggcc cagcgcagca gcgggccgac attggcggtc aggcattcgc
     9061 ccaggcccgt cagcgcgtat tcgaccgtga ccggcaccgt ggcgatcacc gtgcggctga
     9121 ccagcccgtc gcgctccagc cgcttcagca cctggctgag caccttttgc gacagcccgt
     9181 ggatgtcgcg gcgcagcgcg ttgaagcgca ccggcccctc gcttagccgg tgcagcacca
     9241 gcagcgccca cttgtcgcca acatggtcca gcacggcgcg ggccgggcat tcggactggt
     9301 agacatcata agccatcgca gcgtcctgtc gggcggttac cgggcgggta ccttgttccc
     9361 ggaaagtgcc ctctttccat gtttcccata gaccctatgt agcgattcgt catctagtta
     9421 ctttttgaaa ccaacagaca ggacgaaacc acatggcaca gacggttgtt atctatcact
     9481 cgggctatgg ccacaccaag gtcgtggccg aagcggttgc cgccggcgcg cagggccagc
     9541 tgatcgcgat cagccccgaa ggcgacattt ccgaggacga ctgggccgcg ctcgacgccg
     9601 ccgatgcgat cgtcttcggc gcgccgacct acatgggcag cgccagctgg cagttcaaga
     9661 aattctccga cgccacctcg aagcgctggt tcacccgcga ctgggccgac aaggtgttcg
     9721 gcggcttcac caattcggcc agcccctcgg gcgacaagca ggtcacgctg atccagatgc
     9781 agaccctggc cagccagcac ggcgggatct gggtcagcct gggcctggcg cccagcaacc
     9841 acaaggcggc gaccgcggcg gacgtgaaca acctgggcgg ttcggtcggc ctgctggtgc
     9901 gctcgccctc cgatgcctcg gtggacgagg tgcacagcgg cgatctggaa acggcccggc
     9961 tctatggcac gcgggttgcc gggatcgccg cacggctgaa gggctgatcc tcttcagcca
    10021 accagccagg tccacatcat ccgcccgggc aggaacgcaa acaggcccgg gatggtcagc
    10081 gcgccgaggt aaaagccgac caactgcttg cggtggccgg caatatcgcc ccggcgcgcg
    10141 gtggccacgg tcttccacgc accccacagc gtcatcggca cgaagatgtg gatcgggctg
    10201 agctgaccgc cattgatctg gcggatgaac agcgcggaca gggcggtcag gatcatcagc
    10261 acgacccaga ccttgcccca cagcttgtgc cgcgcggtgc ccttgcggct gagcatcagc
    10321 caggccccca gcggcaccgc cggcagcacg gtggccagat gcagcatcac cgccagatcg
    10381 cgcggcgcgg catgggccgg ggccagcccg gcggcaaagc ggaccagccc gattgcgcag
    10441 gcggcggtaa agccgacccc gaccaggccg accgcggcgc ggaccagcgg tccgggttcg
    10501 taggcggcat agggcacggc cgggcggcgg gcggcgcgga tcggtgaagc ggcggtggtg
    10561 gcggtcatcg gaaagtctcc ccggttggtc tgccccctct cctgccgccg ccccgttcgc
    10621 cggcaagcgg ccttgcgcgg acggagcgcg gcgctgcgtg aatggtgcgc aaatccggga
    10681 cctggccact ttacgaagcg cgaacgggcg ctaccttgcc cccgcaatga gccaggccgc
    10741 cccgcccccc agcccccatc ccattcgcca gatcgtgctc gatctggggg tcatgaccgt
    10801 gatcgggatc ctgctcgcgc tgctcgggcc gttcggcagc tttgaactgc ccttcgccta
    10861 ccggctggtc tactggctcg gcctcgcctg ggccggctat gcctgttacc gcccgatcgg
    10921 cgggctgatc acccggctcg gcccggtgct ggacctgccc gactggtcgc tgtggctgat
    10981 cggctgcctc gtggcgaccg cgccgatgac ggtggtggtc tggctggttg gcgccctgcc
    11041 cccaccgctg gagctgccct cgctcgaagc ctggatcgtg acctacggct atgttctggc
    11101 gatcggcgcg gtggtgacca cggtgttcta tctggtccag tccaagcccg ccgccgcgcc
    11161 ggagccgata gcggttgcag cgcagcccga tcccctgccc gctgcacccg ccgccccgcc
    11221 gcgggcccgc ctgctcgacc ggctgccgcc gcggctgggg gacgagattg tcgcgctgga
    11281 aatggaggac cattacctgc gggtccacac ccggctgggc agcgatctga tcctgctgcg
    11341 cctgcgcgat gccctggccg aactggacgg gctggacggg gcgcaggtcc accgcagctg
    11401 gtgggtcgcg cgcggcgggg tggagcgggt ggtccgcgac gggcgcaatg tcgcgctgct
    11461 gctccacggc gggctggagg ccccggtggc gcgcagccag gtcgccgtgc tgaaagccgc
    11521 cggctggctc tgatacgaaa aagggccggc ccttcccgca ggaagagccg gcccctttcg
    11581 tcaggacctc aagctcagtt gaggtcgaag cggtccgcgt tcatcacctt ggtccaggcc
    11641 gccacgaagt cgcgcacgaa cttcccggcg ttgtcgtcct gggcatagag ctcggcatag
    11701 ccgcgcagga tcgagttgga accgaacacc agatcaaccc gggtcgcggt gtgcttgacc
    11761 ttgccggtct tgcgctcgac cagttcgtag aggttccggc ccttcggcac ccagcgatag
    11821 gccatatcgg tgagggtgac gaagaagtcg gcggtcagcg cgcccggacg gtcggtaaac
    11881 accccgtggg cgctgccgcc gtggttgacg cccagcgcac gcaggccgcc gaccagcacg
    11941 gtcatttccg ccgcggtcag ccccatcagc tgggcccggt cgagcagcag ctcttccggc
    12001 tgcacggcgt aatcgccctt cagccagttg cggaacccgt cggccagcgg ctcgagcacg
    12061 gcgaagctgt ccacgtcggt ctgctcggcg ctggcatcgc cgcggcccgg cgcgaagggc
    12121 accgggacgc ggtggccgcc ggcctcgatc gcccgctcca gccccacatt gccgcccagc
    12181 acgatcgtat cggccaggct ggcgccggcc tgcgccgcca gcggttccag caccgccagg
    12241 acccgggcca gccgctcggg ctcgttcccg gcccagtcct tctgcggagc caggcggatg
    12301 cgggcgccgt tggccccgcc gcgcttgtcc gacccgcgga aggtgcgggc gctgtcccag
    12361 gcggtggcga tcaggtcggc cgccgaaagc ccgctggcgg cgatcttcgc cttcagcgcg
    12421 ccaacgtcat aggtgctcga acccgccgga accggatcct gccagatcag gtcttccgcc
    12481 gggacccagg ggcccaggta gttggccttg gggcccatgt cgcggtgggt cagcttgaac
    12541 caggcgcggg cgaagacttc gcagaaatag gcgtgatcgt cgcggaaccg ctcggagatc
    12601 ttgcggtatt ccggatcgac cttcagcgcc atgtcggcat cggtcataac cggcatggtg
    12661 cggatcgcgg gatcttccac gtccaccggc atgtcgtcgg tcgcgatgtt caccgggcgg
    12721 tactggttcg ccccggccgg gctcttggtc agttcccaat cgtgcttgaa cagcatgtcg
    12781 aaatagccgt tgtcgaacac ggtcgggttg ctggtccagg cgccttcaac gccgctggtc
    12841 acggtgtcgc ggcccacgcc gcggccggtc ttgttgagcc agccgaagcc ctggtcctcg
    12901 atctcgccgc cttcgggcgc cgggccgagc acgccggcat cgccgttgcc gtgggccttg
    12961 ccgacggtgt ggccgcccgc ggtcagcgcc acggtttcct cgtcgtccat cgccatgcgg
    13021 ctgaaggtga tgcgcatgtc gcgcgcggtc ttcagcggat cgggctgccc gtcaacgcct
    13081 tccggattga cgtagatcag gcccatcatc accgcggcca gcgggttttc cagctcgcgg
    13141 tcacccgacc agcgggtgcc ttcgctcccc gtaggcgcgg tccattcctt ttccgcgccc
    13201 cagtagatgt ccttttccgg gtgccagatg tcctcgcggc caaagccgaa gccgaaggtc
    13261 ttgaagccca tcgattcgta ggcgacgttg ccggccagga tgaacaggtc ggcccaggac
    13321 agggcgttgc cgtacttctt cttgatcggc cacagcaggc ggcggccctt gtcgaggttg
    13381 ccgttgtcgg gccaggagtt gagcggagcg aagcgctggt tgccggtgct ggccccgccg
    13441 cgcccgtcgg caatgcggta agtcccggcg gcgtgccagg cgaggcggat catcaggccg
    13501 ccgtagtggc cccagtcggc cggccaccag tcctggctgt cggtcatcag cgcggtcaga
    13561 tcggccttca gcgcatcgta atcgagcccc ttcacggcct cgcggtaatc gaagtccggg
    13621 ccgagcgggt tggtcttggc atcgtgctgg tggaggatgt cgagattgag ggcattgggc
    13681 caccaggccg tgggcgagct gtccgcccgg gtgctgccgc cgtgcatcac ggggcatttg
    13741 gtggaagcgt tcatcgcggt ctctccttcg tcataaatcg atgaagtcga tctaccagcc
    13801 ccttatagtt cggctaaacc gattaaagtc gtttcagtta tcgggcccgg cgattgatcg
    13861 aacgatgcaa gggccggact gcggcttgca ggcagcgaat ctgccgccta aagcccgctg
    13921 accttacgac atccgaggcc tttcccgtgt atgaaaaaac ccacgcgctg atgcagcgcg
    13981 gtatcgactt cctggggagc gagcacgcca tcctgtgcgg agccatgagc tgggtgtccg
    14041 aacgcaacct ggtggcggcc atgtc
//
LOCUS       DASUDV010000224         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1354800,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000224 DASUDV010000000
VERSION     DASUDV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354800"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFV30_04855"
     CDS             complement(<1..703)
                     /locus_tag="VFV30_04855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX4847450.1"
                     /translation="MIDWLLRPPREAPSLEVAGRHLPVVLRRMANARRMTMRLAPDGS
                     EVRITLPSWGRTAEALEFARKRAGWIAGQLAAVPEPVSLGDGALIAYLGEQFVLRHDP
                     AAQRRVRVTASELLVGGPSESLGPRLARWLEGEARALLREDLEFYCERAGRPAPRLML
                     SRARRRWGSCARDGTIRINWRLVMAPEPVRRSVVAHEVAHLVHFDHSPAFHDLLDELF
                     EGDVRAANRWLKREGR"
     gene            complement(696..1154)
                     /locus_tag="VFV30_04860"
     CDS             complement(696..1154)
                     /locus_tag="VFV30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX4847451.1"
                     /translation="MSGLRDKFWELPLKALDRAEWEALCDGCGQCCLHKAEDEDTGAI
                     YPTNVACRLLDIHSARCRNYAGRKAIVPDCVQLTPRLAGRIEWLPETCAYRLRAEGEP
                     LPPWHYLICGDPEAVHREGYSVRGSAISEDLAGPLEQHVILPPGVELADD"
     gene            complement(1151..1768)
                     /locus_tag="VFV30_04865"
     CDS             complement(1151..1768)
                     /locus_tag="VFV30_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4847452.1"
                     /translation="MNRHAMTKPRLIVSLCAALALSACNPSPKGTATAMHGTDLAGAA
                     IGGPFTLSDKDGKPVTWDQFKGQYRMVYFGYTFCPDACPMDVGVMMQGFNRFAKNDPA
                     AAAKVQPIFITIDPARDTPARVGEFGAAFSPRLLGLTGTQDQVDGAVKAFKAVAQRGK
                     DTPGGYLMDHSRMVYLMDPDGRPLAALPVDKGPEEVAADLALLVK"
     gene            1843..>2324
                     /locus_tag="VFV30_04870"
     CDS             1843..>2324
                     /locus_tag="VFV30_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4847453.1"
                     /translation="MPKFRLTALFAAMTLTLGLAVPMPAAAQFSESYKFLDAVKKKEG
                     QEVTDMLAEGSSSLINTRDLTSGESALHLVTKRRDLTWMQFLIGKGANVNIRDAKGTT
                     PLVVACNLGFVEGAELLVKYGARVDESNTAGETPLITAVHNRNIELMRILLKAGANPD
                     R"
BASE COUNT          414 a          769 c          747 g          394 t
ORIGIN      
        1 tgcggccctc gcgcttgagc cagcggttgg cggcacgtac atcgccttcg aacagttcgt
       61 cgagcagatc gtggaacgcc ggactgtggt cgaaatgaac caggtgcgcc acctcgtgcg
      121 ccaccaccga tcggcgcacc ggttctggcg ccatgaccag ccgccagttg atccggatcg
      181 tcccatcccg agcgcacgat ccccaccggc gccgggcgcg gctcagcatc agtcggggcg
      241 cagggcggcc agctctttcg caatagaatt cgagatcttc cctgagcagg gcgcgggcct
      301 caccctccag ccagcgggcc agcctggggc caagcgattc gctgggcccc ccaaccagca
      361 gctcgcttgc cgttaccctt acccgccgct gggctgcagg atcgtggcgc aacacgaact
      421 gttcaccaag gtaggcgatc agcgcgccat cacccaggct gaccggctcg ggcacggctg
      481 ccagttgccc ggcgatccag cctgcgcgct tgcgggcaaa ctccagcgcc tcggcggtgc
      541 gtccccagct gggcagggtg atccgcactt cgctgccatc gggggcaagc cgcatcgtca
      601 tccgccgggc attggccatg cggcgcagca ccaccggcaa atggcgcccc gccacctcga
      661 gcgagggcgc ctcacgcggg gggcgaagca gccagtcaat catcggccag ttccactcca
      721 ggcggcagga tcacgtgctg ttcgagcgga ccggccagat cctcgctgat cgcgctgccg
      781 cgcaccgaat agccttcgcg gtggaccgcc tcagggtcgc cgcagatcag atagtgccag
      841 ggtggcaatg gttcgccctc ggcgcgcaag cgataggcgc aggtctcggg cagccattca
      901 atccgccctg ccagccgcgg ggtcagttgg acgcaatcgg gcacgattgc cttgcgaccg
      961 gcatagttgc ggcagcgcgc cgaatggata tcgagcagcc ggcaggcgac attggtggga
     1021 tagatcgcgc cggtatcctc atcctcggcc ttgtgcaggc agcactgtcc gcagccgtcg
     1081 cacagcgctt cccattcggc gcggtccagc gccttgagcg gcaattccca gaacttgtcg
     1141 cgcaggccgc tcatttcacc agcagggcca ggtcggcggc gacctcttca ggacccttgt
     1201 cgaccggaag cgcggccagc gggcgaccat cgggatccat caggtaaacc attcggctgt
     1261 gatccatcag gtatccgcca ggcgtgtcct tgccgcgctg ggctaccgcc ttgaacgcct
     1321 tcactgcgcc gtcgacctgg tcttgcgtgc cggtcaggcc cagcaggcgc ggtgaaaacg
     1381 ccgcgccgaa ttcgcccacc cgggccggcg tgtcgcgcgc cggatcgatg gtgatgaaga
     1441 tcggctggac cttggcggca gccgcgggat cgttcttggc gaagcggttg aagccctgca
     1501 tcatcacccc gacgtccatc ggacaggcat ctgggcagaa ggtgtagccg aaatagacca
     1561 tccggtactg gcccttgaac tgatcccagg taaccggctt gccgtccttg tcggacagcg
     1621 tgaacgggcc gccgatcgcc gcgccggcaa gatcggtccc gtgcatggct gtggcagtgc
     1681 ccttgggtga aggattgcag gccgaaaggg ccagcgcggc gcaaagtgaa acgatcaatc
     1741 ggggcttggt catagcgtgg cggttcatgc tctgctaaac cggcgcatgc aaggtgcctt
     1801 tcgggccggg cgattctgcc cgcaacagga ggattgaggt tcgtgccgaa gtttcgtttg
     1861 actgcgctgt tcgcggcgat gacgctgact ctgggtttgg ccgtgccgat gccggctgcc
     1921 gcgcagtttt ccgaaagcta caagttcctc gacgcggtca agaaaaagga aggccaggaa
     1981 gtcaccgaca tgctggccga aggtagctcc agcctgatca acacccgtga ccttacctcg
     2041 ggcgaatccg ccctgcatct ggtcaccaag cgtcgcgatc tcacctggat gcaatttctg
     2101 atcggcaaag gcgccaacgt gaacatccgc gatgccaagg gcacgacccc gctggtggtt
     2161 gcctgcaacc ttggcttcgt cgaaggggcc gaactgctgg tgaaatacgg cgcgcgggtc
     2221 gatgaaagca acaccgccgg cgaaacgccg ctgattactg cggttcacaa ccgcaacatc
     2281 gaactgatgc ggattttgct gaaggccggg gccaaccccg atcg
//
LOCUS       DASUDV010000225         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1862806,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000225 DASUDV010000000
VERSION     DASUDV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862806"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..587
                     /locus_tag="VFV30_04875"
     CDS             51..587
                     /locus_tag="VFV30_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4847454.1"
                     /translation="MPRLTVNGRPVEFEMDPQTPLLWGLRDAANLTGTKFGCGVGDCG
                     ACMVLIDGEALRSCLVTLAEAEGRTVTTIEGLSPDRSHPVQQALVAEQAIQCGFCTPG
                     IAIAAAALLSRNADPTPEDIKAAVPNLCRCGVYPRLVRAVQRAGRVMRRVETISAAPA
                     PGITPEDAARAVPALKSD"
     gene            604..1884
                     /locus_tag="VFV30_04880"
     CDS             604..1884
                     /locus_tag="VFV30_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HEX4847455.1"
                     /translation="MIETPLTLPCGAVLPNRLAKAAMTEGLADPQGRPGKELERLYRI
                     WGEGGCGVLLSGNVHIDADHLERPGNVIIDRPADAAMLAGLRRWTAAAKAGGAQFWAQ
                     ISHAGRQTMKPVNPRPKAPSAVKLGLPGGQFGEPVALTEAEIEELIDRFARAAVICAE
                     GGFDGVQIHAAHGYLISQFLSPRANQRTDRWGGSLENRARFLLEVTRATRAAVPAGFA
                     VTVKLNSADFQRGGFAFEDSLQVAQWLEAAGVDLIEISGGTYEQPRLLGIEGMEPVEQ
                     PGVAASTLAREAYFVDFAQAMKRVLKVPLMVTGGFRTRAAMEEALRQGGADVIGLGRP
                     LCAEPAGAAQLLAGAESLPSPEKHLALFPDWLAVLRKLKPLRAIDSFAVQYWFYCQIY
                     ALGREGHTDPGKSVFASLLEVEKTHKAWLKARRG"
     gene            complement(1881..>2426)
                     /locus_tag="VFV30_04885"
     CDS             complement(1881..>2426)
                     /locus_tag="VFV30_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847456.1"
                     /translation="KWDITGSLAFTAAVFRLDRSNTRATDPLTGNAVLTGKSRTEGVE
                     LALVGRVTDGLDVSLGYALQDGEIRATTTAAPAGRKLAQLPRHQASAWARYNFTDRMG
                     LGLGVVHQSSQFATISNAVRLPAFTRIDAAFYYDVSERLSLQVNVENLTDTGYYPSAH
                     TDNNITVGEPLNAKVTLRLKF"
BASE COUNT          351 a          819 c          879 g          377 t
ORIGIN      
        1 ggcggatcga caaggtcgat cgcagctggc gggactagtc gcgccctgca atgccgcgcc
       61 ttaccgtcaa cggccgcccg gtcgaattcg aaatggatcc gcagaccccc ttgttgtggg
      121 ggctgcgcga tgctgccaac ctgaccggca ccaagttcgg ttgcggggtg ggcgattgcg
      181 gcgcctgcat ggtcctgatc gacggggagg cgctgcgctc gtgcctggtg accctggccg
      241 aggccgaggg ccggaccgtg acgacgatcg aggggctttc gcccgaccgg tcgcacccgg
      301 tccagcaggc gctggtggcc gaacaggcga tccagtgcgg gttctgcacc cccgggatag
      361 ccatcgccgc ggcggcgctg ctgtcgcgca acgccgatcc gaccccggaa gacatcaagg
      421 ccgccgtgcc caacctgtgc cgctgcgggg tctatccgcg gctggttcgc gcggtccagc
      481 gcgccggacg ggtgatgcgc cgggtcgaga cgatcagcgc cgcgccggcg cccgggatca
      541 ctccggaaga cgctgcgcgc gcggttccgg ccctgaaatc cgattgaccc agaaagcctg
      601 ccgatgatcg agaccccgct gaccctgccc tgcggcgccg tgctgcccaa ccgcctggcc
      661 aaggcggcga tgaccgaagg cctggccgat ccgcagggcc ggcccggcaa ggagctggaa
      721 cggctctacc ggatctgggg cgaaggcggc tgcggggtgc tgctgtcggg caatgtccac
      781 atcgatgccg atcacctgga gcggcccggc aacgtgatca tcgaccgtcc ggccgatgcc
      841 gccatgctgg ccggcctgcg tcgctggacc gccgccgcca aggcgggcgg ggcgcagttc
      901 tgggcccaga tcagccacgc cggacggcag acgatgaagc cggtcaaccc ccggcccaag
      961 gcgccttcgg cggtcaagct gggtctgccc ggcgggcagt tcggcgagcc ggtggcgctg
     1021 accgaggccg agatcgaaga gctgatcgac cgctttgccc gggccgcagt gatctgcgcc
     1081 gaaggcgggt ttgacggggt gcagatccac gccgcccacg gctatctcat ctcgcagttc
     1141 ctcagcccgc gcgccaacca gcggaccgac cggtggggcg gcagcctgga aaaccgcgcc
     1201 cggttcctgc tcgaggtgac tcgtgcgacc cgcgccgcgg tgcccgcggg ctttgccgtg
     1261 acggtcaagc tcaacagcgc cgatttccag cgcggcggct ttgcctttga ggacagcctg
     1321 caggtggccc agtggctgga ggccgcgggc gttgacctga tcgagatttc gggcgggacc
     1381 tatgagcagc cccggctgct ggggatcgaa gggatggagc cggtcgagca gcccggcgtt
     1441 gccgcctcaa ccctcgcgcg cgaggcctat ttcgtcgatt ttgcccaggc catgaagcgg
     1501 gtgctgaagg tgccgctgat ggtcaccggg ggcttccgca cccgcgccgc gatggaagag
     1561 gcgctgcgcc agggcggggc cgacgtgatc ggtctgggcc ggccgctctg cgccgaaccg
     1621 gccggcgcgg cccagttgct cgccggggcc gagagtctgc ccagcccgga aaagcacctg
     1681 gcgctgttcc cggattggct ggccgtgctg cgcaagttga agccgctgcg cgcgattgac
     1741 agctttgccg tgcagtactg gttctattgc cagatctatg cgctgggccg cgaaggccac
     1801 accgatcccg gcaagtcggt cttcgcctcg ctgctcgagg tggagaagac ccacaaggcc
     1861 tggctcaagg cccgccgcgg ctagaacttc agccgcagcg tgaccttggc gttcagcggc
     1921 tcgccgacgg tgatgttgtt gtcggtgtgg gcgctggggt aatagccggt gtcggtcagg
     1981 ttctcgacat tgacctgcag gctcaggcgc tcgctgacat cgtaatagaa cgccgcgtcg
     2041 atccgggtga aggccggcag gcgcaccgcg ttgctgatcg tggcgaactg gctggactgg
     2101 tgaaccacgc ccaggcccag gcccatccgg tcggtgaagt tgtagcgcgc ccaggccgac
     2161 gcctggtggc gcggcagctg cgccagcttg cgcccggccg gggccgcggt ggtggtggcc
     2221 cggatctcgc cgtcctgcag ggcatagccc agcgacacgt cgagcccgtc ggtcacccgg
     2281 ccaaccagcg ccagctccac cccttcggtc cggctcttgc cggtcaggac ggcgttgccg
     2341 gtcagcggat cggtggcgcg ggtgttgctg cggtccagcc ggaacacggc ggcggtgaag
     2401 gccagcgatc cggtgatgtc ccactt
//
LOCUS       DASUDV010000226         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_559371,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000226 DASUDV010000000
VERSION     DASUDV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559371"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="VFV30_04890"
     CDS             <1..1694
                     /locus_tag="VFV30_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4847457.1"
                     /translation="RIGGGSAFFIDSAMAVPQLLKAGVDYILLDYLAEGAMGLLGRAR
                     LADPAAGYPSDLLDVHVGPFLKEIAAGGTRIVTNAGGVNPEGLAAALRTRIAALGLSL
                     KVACVAGDDLTGQLEGLADKVDMFTGAPFPASGVTSCNAYLGAFPISEALQRGADIVV
                     TGRVVDSALALGPLIHEFGWQRGEWELLSGGTLAGHLIECGAQVTGGTFTDWREVEGW
                     ATIGAPIAECHADGTCIITKPVGSGGLVSKGTVAEQLLYETSDPQCYFVPDVTCDWSG
                     VSLEEIGPNRVRVSGARGYPPPETLKACVTWDDGWRATVWQPVIGPAAREKAEKQAAA
                     LFERSRLMLRARNLADWRRSEAVLAGGGEEVLLKLVVEHDEMLPAQMFLREQFAAISA
                     MAPGTSVAFAPTVQPMMNLLSLAVPRDLAPPLLDGEMLEDSHPAWFDPAAIERPAAPA
                     LPVPGQTHTTLESLAWARSGEKGETINVGVIARRGENLSALRAALSEDALREWLPDLG
                     PFTLTVYDLPGFDAINLVLKGALPGGLNASQRLDPAAKSIAQRLMEFPVRLPQRL"
     gene            complement(1672..>2150)
                     /locus_tag="VFV30_04895"
     CDS             complement(1672..>2150)
                     /locus_tag="VFV30_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4847458.1"
                     /translation="GLYLAALGNYENGTLDSAYEMLTHPDCVPALGDGGAHYGAICDA
                     SYSTFLLRQFARGGKQAIPVEQAVHMLSAKAARAVGLDRGVLKVGAKADCNVIDLDKL
                     ELHLPRIVHDLPAGGRRLDQTAKGYAATLVSGQVIRRFDEATGARPGQLIRGAAAA"
BASE COUNT          365 a          692 c          722 g          371 t
ORIGIN      
        1 tccggatcgg cggaggcagc gccttcttca tcgacagcgc gatggccgtg ccgcagttgc
       61 taaaggccgg ggtcgattac atcctgctcg actatctcgc cgagggcgcg atggggctgc
      121 tgggccgggc caggctggcc gacccggcgg cgggataccc ttccgacctg ctcgacgtgc
      181 atgtcgggcc gttcctgaaa gagatcgctg ctggcggcac gcggatcgtc accaacgccg
      241 gcggggtcaa tcccgagggg ttggcggcgg ccttgcggac acgaattgct gcattggggt
      301 tgtcgctcaa ggtagcctgt gttgccggtg atgacctgac aggtcagctc gaagggctgg
      361 cggacaaggt cgacatgttc accggcgctc ccttccccgc ttccggggta accagttgta
      421 acgcttacct gggagcgttt ccgatctccg aagctttgca acgcggggcc gacatcgtgg
      481 tgaccgggcg ggtcgtcgac tcggccctgg cgctcggccc actgatccac gaattcggct
      541 ggcagcgcgg cgaatgggaa ctgctttcgg gcgggaccct ggccgggcac ctgatcgaat
      601 gcggtgcgca ggttaccggc ggaaccttca ccgactggcg cgaggtcgaa ggttgggcaa
      661 cgatcggtgc gcctatcgcg gaatgtcatg ccgacggaac ctgcatcatc accaagccgg
      721 ttgggagcgg cgggttggtc agcaagggca ccgtggcaga gcaattgctg tatgaaacct
      781 cggatccgca atgctatttc gtccctgacg tgacctgcga ctggtcgggc gtttcgctcg
      841 aggaaatcgg accgaaccgg gtgcgggtga gcggggcgcg cggctaccct ccccccgaaa
      901 cgctcaaggc ctgcgtcacc tgggacgatg ggtggcgggc cacagtgtgg cagccggtga
      961 tcggtccggc agcgcgggag aaggctgaaa agcaggccgc cgcgctgttc gagcgcagcc
     1021 gcttgatgct gcgcgcacga aacctggccg attggcgtcg cagcgaagcc gtactggccg
     1081 gcggaggcga ggaagtgttg ctgaaactgg tggtggaaca cgacgaaatg ctgcccgcgc
     1141 agatgttcct gcgcgaacag ttcgccgcga tatcggccat ggcgcccgga accagcgtgg
     1201 cctttgcgcc cacggtccaa ccgatgatga acctgctgag ccttgcggtc ccgcgcgacc
     1261 ttgcgccgcc gctgctcgat ggcgagatgc tggaggacag ccatccggcc tggttcgatc
     1321 cggccgcgat tgagcgtccc gctgcccccg cgctgcccgt tcccggccag acccatacca
     1381 cgctcgaaag tctggcctgg gctcgatcgg gggaaaaggg agaaacgatc aatgtcggcg
     1441 tgatcgcaag acgcggcgag aacctgtctg cactgcgcgc cgcgctgagc gaggatgccc
     1501 ttcgagaatg gctgcccgat ctggggccct ttaccctgac cgtctacgac ctgccaggct
     1561 tcgatgcgat caacctggtt ctgaagggtg ccctgcccgg cggactcaac gccagccaac
     1621 ggctcgatcc cgccgcaaag agcattgccc agcggctgat ggagttcccg gtcaggctgc
     1681 cgcagcgcct ctgatcaatt gccctggacg cgcaccggtg gcctcgtcga agcggcggat
     1741 cacctgcccc gaaaccagcg tcgcggcata acccttggcg gtctggtcaa gccggcgccc
     1801 cccggctggc aagtcatgga cgatgcgcgg taggtgcaat tccagcttgt cgaggtcgat
     1861 cacgttgcaa tcggccttgg ccccgacctt cagcacgccg cggtcaagcc cgacagcccg
     1921 cgccgccttg gccgaaagca tgtgcaccgc ctgttcgacc gggatcgcct gcttgccgcc
     1981 gcgcgcgaac tgcctcagca ggaaggtcga ataactggca tcgcagatcg cgccataatg
     2041 cgccccgcca tcgcccaacg ccggcacgca atcggggtgg gtcagcattt cataggcgga
     2101 atcgagcgtt ccgttttcgt aattgcccag tgccgccaga tagagcccct
//
LOCUS       DASUDV010000227         5807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1133661,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000227 DASUDV010000000
VERSION     DASUDV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133661"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1256
                     /gene="lptF"
                     /locus_tag="VFV30_04900"
     CDS             48..1256
                     /gene="lptF"
                     /locus_tag="VFV30_04900"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX4847459.1"
                     /translation="MKSIPAIDRYIFRLVLMPMLAVFVVAASLLVLDKMLKLFEFVGN
                     EGGPISVVFRMLANLLPEYASLAIPLGLMLGILFAFRKLATTSELDVLRAVGMSYTRL
                     LRVPYAITLVLAGVNLLIVGYLQPLSRYWYEQLDFELRSGALGASIKVGEFTTLQDKM
                     ALRIEQSKDNGRQLIGIFARFSSPKGEVLTISAREGRFLSLKDSPDTIILRLTDGQII
                     QDEPGASPRVLSFSRHDMPIDLPAIQEFRERGGKEREYILPELLRIGWSDQVTQKVRN
                     ETRASLNYRLVEVVMMLLLPLLAVALAVPPKRSSSPLGVFVSIVMVVAYHKVNEYGQA
                     AAAQGRFPPELILWGPFVVFGALIVWMYWRVAYVPGGQPIGALERFFANLGKRLTALW
                     KRRRGAAGAA"
     gene            1256..2353
                     /gene="lptG"
                     /locus_tag="VFV30_04905"
     CDS             1256..2353
                     /gene="lptG"
                     /locus_tag="VFV30_04905"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX4847460.1"
                     /translation="MLAEIFPSRTLTWYLAKLFVGRIVGVLILLVLVLQMMQLLSESG
                     DILAVPGNGEAELWYYVSLNVPQLIARFLPYSVLLATLFTFWPLNQNSEVIAMRAAGL
                     SAHQILAPLLLTAGVVSLASFTFNETVVTRAAGTLKEWTKLKYHPIPKDSGVRSNVYL
                     RDGTDILNAASVTGTGTGIVMTGVTWYRRDAGGMVVEQLRSPRATWAGPGWQLESPQR
                     FDVRTTTLEPVPSQVVGRGISPDQVRISRVDPDGQNVFELARSIDALKAAGRRTSELE
                     AKWWHKFSAPLSALLMPLLGSVAAFGLARSGKLLVRAVAGMALGFAYFVVDNAALAMG
                     NFGGYPALIAAWAPFLLFALVGETVLVRTEE"
     gene            complement(2497..2976)
                     /locus_tag="VFV30_04910"
     CDS             complement(2497..2976)
                     /locus_tag="VFV30_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX4847461.1"
                     /translation="MDVDAAISRALSAVGQGIRYKLGKGGMKPGSPTPAAAGQCDCTG
                     FVSWCFGMSRHTPDAFYVAFNGGWIETTAVWTDIGRSVGIFEQLDKPRRGAVVVFPDA
                     LGAQGHIGILTSASKVIHCSKGNDTRTGDAIQETGLAVFNRPDVRYGWFVGLGPIGG"
     gene            complement(3000..4043)
                     /locus_tag="VFV30_04915"
     CDS             complement(3000..4043)
                     /locus_tag="VFV30_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847462.1"
                     /translation="MACEEQQAALDAEIQAIAEIAKEETDRLTAEADAALKNAGEALG
                     EDGLSGAVGAAVGGAIGSTAGPGGTAVGAVIGRELGKLFVISVEMRRTRMVIGLPTVR
                     MTSQRVAFDLPSVTMRNRDIIFHLPSVRMNRVKIGEKPETTCSGGGWLGLPQCTVRWT
                     PIWADVPEPYMQENRIVLAIPEITMQRNDWRFDVPDVRIEDQEILFDVPSIKIESRQD
                     AAERAADAALELTRQFQDRAAGLAKAAKERAKDRIAPRISGVFACHRANLEQQLVSLP
                     QRFDPVIDQLNGAISGMAGRGVPSTDDDYAQMVAQRDAMLQQRRTLVDTLHAQIADLS
                     AKEAEAINALIAS"
     gene            complement(4143..5201)
                     /locus_tag="VFV30_04920"
     CDS             complement(4143..5201)
                     /locus_tag="VFV30_04920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX4847463.1"
                     /translation="MTVQENIAAAPAKSAIPDDMAMLRAAVELTRDISAARPEIYWPD
                     MLVSAALGYAGLAGAILLDGWAAWACGALAVFTLYRALLFIHELTHIHKTALPGFRFV
                     WNLLVGIPMLTPSFMYEGVHTLHHARTRYGTAEDPEYLPLALMKPWSLPAFLLVSLLL
                     PVGLLIRSAVLVPLGVIVPPLRKLVWERASSLSINPDFRRRPPEGDFARMVFWQELGA
                     SLWAWALLASTQVWGWRWLAIAVAVVAVTALLNQIRTLVAHLWENDGDAMTVTGQYLD
                     SVNVPPPNLLSPLWAPVGLRYHATHHLLPSMPYHSLGEAHRRISAHLGLGSTFEKASY
                     TGFMPLLAKIARSTMVRG"
     gene            5304..>5807
                     /locus_tag="VFV30_04925"
     CDS             5304..>5807
                     /locus_tag="VFV30_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4847464.1"
                     /translation="MANQQINVAPVASKADLNAFIDLAYRLNRSDPNWVAPLRFEVAE
                     LLTPGKNPFFDHARVQLFLARRGDAVVGRISAHIDELALAQPAEQGMGPGTGNWGLLE
                     AEDEAAAQALIARAEDWLREQGMTRVLAPLSMSVWEEPGQLTKGFDHPPTVMMGHQPQ
                     RYQHYIEA"
BASE COUNT          939 a         1954 c         1951 g          963 t
ORIGIN      
        1 ggggcggtta tgggacaggc cagcacacaa gagcgaccgg gccccacttg aaatcgatcc
       61 ccgccatcga ccgttacatc ttccggctgg tgctgatgcc catgctggcg gtctttgtcg
      121 tcgccgcgtc gctgctggtg ctcgacaaga tgctcaagct gttcgaattc gtcggcaacg
      181 agggcgggcc gatttcggtc gtcttccgga tgctggccaa cctgctgccg gaatacgcca
      241 gcctggcgat cccgctgggg ctgatgctgg gcatcctgtt cgccttccgc aagctggcca
      301 ccaccagcga gctcgacgtg ctgcgcgcgg tcggcatgtc ctatacgcgg ctgctgcggg
      361 ttccctatgc gatcacgctg gtcctggccg gggtcaacct gctgatcgtc ggctatctgc
      421 agccgctgtc gcgctattgg tacgaacagc tcgatttcga actgcgctcc ggcgcgctgg
      481 gggcctcgat caaggtcggg gaattcacca ccctgcagga caagatggcc ctccggatcg
      541 agcaatcgaa ggacaacggc cgccagctga tcggcatttt cgcgcggttc tcctcgccca
      601 agggcgaagt gctgacgatt tcggcccgcg aagggcgctt cctctcgctg aaggacagcc
      661 cggacacgat catcctccgg ctgaccgacg gccagatcat ccaggacgaa cccggtgcca
      721 gcccccgggt gctgagcttc tcgcgccacg acatgccgat cgatctgccc gcgatccagg
      781 aattccgcga acgcggcggc aaggagcgcg aatacatcct gcctgaactt ctccggatcg
      841 ggtggagcga ccaggtgacc cagaaggtcc gcaacgagac ccgggccagc ctgaattacc
      901 ggctggtcga agtggtgatg atgctgctct tgccgctgct cgccgtggcg ctggccgtgc
      961 cgcccaaacg gtcgagctcg ccgctggggg tcttcgtctc gatcgtgatg gtggtggcct
     1021 atcacaaggt caacgaatac ggccaggccg ccgcggccca gggccggttc ccgcccgaac
     1081 tgatcctgtg ggggcccttc gtggtcttcg gcgcgctgat cgtgtggatg tactggcggg
     1141 ttgcctatgt ccccggcggc cagccgatcg gcgcgctgga acgcttcttc gccaacctgg
     1201 gcaagcgcct gaccgccctg tggaagcgcc gccgcggcgc ggccggagcc gcctgatgct
     1261 ggccgagatc tttccttcgc ggaccctgac ctggtatctg gccaagctgt tcgtcggccg
     1321 gatcgtgggc gtgctgatcc tgctggtgct ggtgctgcag atgatgcagc tgctgtccga
     1381 aagcggcgac atcctggccg tgcccggcaa cggcgaggcc gagctgtggt actacgtctc
     1441 gctcaacgtg ccgcagctga tcgcccggtt cctgccctat tcggtgctgc tggccaccct
     1501 gttcaccttc tggccgctga accagaacag cgaggtcatc gcgatgcgcg cggccgggct
     1561 ttcggcgcac cagatcctgg cgccgctgct gctcaccgcc ggggtcgtca gcctggccag
     1621 cttcactttc aacgaaaccg tggtgacccg cgccgccggc acgctgaagg aatggaccaa
     1681 gctgaaatac cacccgatcc cgaaggacag cggcgtgcgc agcaatgtct acctgcgcga
     1741 cgggaccgac atcctcaacg ccgccagcgt gaccggcaca ggcacgggaa tcgtgatgac
     1801 cggggtcacc tggtaccgcc gcgatgccgg cggaatggtg gtggagcagc tgcgcagccc
     1861 gcgcgcgaca tgggccgggc cgggctggca actggaaagc ccgcagcgct tcgacgtgcg
     1921 caccaccacg ctggaaccgg tgccgagcca ggtggtcggc cgcggcatct cgcccgatca
     1981 ggtccggatc agccgggtcg atcccgacgg gcagaacgtg ttcgaactgg cccgctcgat
     2041 cgacgcgctg aaggccgccg ggcgccgcac cagcgagctg gaggcgaagt ggtggcacaa
     2101 gttctcggcc ccgctgtcgg cactgctgat gccgctgctg ggttcggtcg cggcctttgg
     2161 cctcgcccgg tcaggcaagc tgctggtccg cgccgtggcc gggatggcgc tgggctttgc
     2221 ctatttcgtg gtcgacaacg ccgcgctggc gatgggcaat ttcggcggat accccgccct
     2281 gatcgctgcc tgggcgccgt tcctgctgtt tgccctggtg ggcgaaacgg tgctggtccg
     2341 gaccgaggaa tgatccttga actggcttcg gcctggccga agccgttgca tcaccggccc
     2401 actcccccgg cccaaccacc ccaagggtac catgtaacgg gtggtagggc cgggggagtg
     2461 ggccggtgat gccgccacgt cagtggcgca aaggactcac cccccaatcg gccccaatcc
     2521 cacgaaccag ccgtaacgca catcgggccg gttgaagacc gcaagtccgg tttcctggat
     2581 tgcgtcgccg gtccgggtgt cgttgccctt ggagcaatgg atcaccttgc tggccgaagt
     2641 caggatcccg atgtgaccct gcgcgcccag cgcatcgggg aagaccacca cggccccgcg
     2701 ccgcggcttg tcgagctgtt cgaagatccc gaccgagcgg ccgatatcgg tccagaccgc
     2761 ggtcgtttcg atccacccgc cgttgaaggc cacgtagaac gcgtccggcg tgtggcggct
     2821 catcccgaag caccaggaca cgaagccggt gcaatcgcac tgcccggccg ccgccggggt
     2881 ggggctgccc ggcttcatcc cgcccttgcc cagcttgtag cggatgccct ggccaacggc
     2941 cgagagcgcc cgggaaatcg cggcatcgac atccattgcg gttctccttg cgcggcggat
     3001 caggacgcga tcagcgcgtt gatcgcttcg gcctccttgg ccgagagatc cgcgatctgg
     3061 gcgtggaggg tgtcgaccag ggtccggcgc tgctgcagca tcgcgtcgcg ctgggcgacc
     3121 atctgggcat agtcgtcgtc ggtgctcggc accccgcggc cggccatgcc ggaaatcgcc
     3181 ccgttgagct ggtcgatcac cggatcgaag cgctgcggca gcgaaaccag ctgctgttcc
     3241 aggttggcgc ggtggcaggc aaagacgccg gagatgcgcg gagcgatccg gtccttggcg
     3301 cgttccttgg ccgccttggc caggccggcg gcgcggtcct ggaactggcg ggtcagctcc
     3361 agcgcggcat cggcggcgcg ctcggccgcg tcctgccggc tttcgatctt gatgctgggc
     3421 acatcgaaca ggatttcctg gtcctcgatc cgcacgtccg gcacgtcaaa acgccagtcg
     3481 ttgcgctgca tggtgatttc cggaatcgcc aggacgatgc ggttttcctg catgtaaggc
     3541 tccggcacgt cggcccagat cggcgtccag cgcacggtgc actgcggcag tcccagccag
     3601 ccaccgccgc tgcaggtcgt ctcgggcttc tcgccaatct tgacccggtt catccggacc
     3661 gagggcaggt ggaagatgat gtcgcggttg cgcatggtca cgctgggcag gtcgaacgcg
     3721 acccgctggc tggtcatccg cacggtcggc aaaccgatca ccatccgggt gcggcgcatt
     3781 tcgacgctga tcacgaaaag cttgcccagt tcgcggccga tcaccgcgcc cacggcggtg
     3841 ccgcccgggc cggcggtcga tccgatcgca ccgcccaccg cggccccgac cgcgccggac
     3901 aggccgtcct cgcccagcgc ctcgccggcg ttcttcagcg cggcatcggc ttcggcggtc
     3961 agccggtcgg tttcttcctt ggcgatttcg gcaatcgcct gaatttcggc gtccagcgcc
     4021 gcctgttgct cttcgcaggc catgttcgtc tcccgtttgc acgcacgcca cagggcgtgg
     4081 gtgttgggag agagactagg gcaaggcgcg ccgggcgaac gtgacgggca tcacgcccgc
     4141 gcttagcccc gcaccatcgt gctgcgggca atcttggcga gcagcggcat gaagccggta
     4201 tagctggcct tttcaaaggt cgaacccagg cccaggtgcg cgctgatccg gcggtgcgcc
     4261 tcgcccagcg agtgataggg catcgacggc agcaggtggt gcgtcgcgtg atagcgcagg
     4321 cccaccggcg cccacagcgg cgagagcagg ttgggcggcg ggacgttgac cgaatcgagg
     4381 tactgcccgg tcacggtcat ggcatcgccg tcattctccc acaggtgcgc gacgagcgtg
     4441 cggatctggt tgagcagcgc cgtgaccgcc accacggcca ccgcgatcgc cagccagcgc
     4501 cagccccaga cctgggtgct ggccagcagg gcccaggccc agaggctggc acccagttcc
     4561 tgccagaaca ccatccgcgc gaaatcgcct tcgggcgggc ggcggcggaa atcgggattg
     4621 atcgacagcg acgaggcgcg ttcccacacc agcttgcgca gcggcggcac gatcacgccc
     4681 agcggcacca gaacggccga acggatcagc aggccgaccg gcagcagcag cgagaccagc
     4741 aggaaggccg gcaggctcca cggcttcatc agcgccagcg gcaggtattc cggatcctcg
     4801 gccgtgccat agcgggtgcg ggcatggtgc agcgtgtgca ccccttcgta catgaacgac
     4861 ggggtcagca tcggaatgcc gaccagcagg ttccagacaa agcggaagcc gggcagcgcg
     4921 gtcttgtgga tgtgggtcag ctcgtgaatg aacagcagcg cgcggtagag cgtgaacacc
     4981 gccagcgcgc cgcaggccca ggccgcccag ccatcgagca ggatcgcccc ggcaaggccg
     5041 gcatagccca gcgcggccga aaccagcatg tccggccaat agatttccgg ccgcgcggcg
     5101 gaaatgtcgc gggtcagctc aaccgcggcg cgcagcatcg ccatgtcgtc gggaatggcc
     5161 gacttggccg gcgcggcagc gatgttttcc tgaacagtca tttgcagttc gatccgttgg
     5221 cgggccggct aagccgaacc ctcggcttga caacctgccc atagcagaga caggtcgcat
     5281 tgaccgtgaa caaaggcagc atcgtggcaa accagcagat aaatgtcgca cccgtggcca
     5341 gcaaggccga cctgaacgct tttatcgatc ttgcttatcg gctgaaccgt tcggacccga
     5401 actgggtggc gccgctgcgc ttcgaagtgg ccgaactgct gacgccgggc aagaacccgt
     5461 tcttcgacca tgcccgggtc cagctgttcc tggcccgccg cggcgatgcg gtggtcggcc
     5521 ggatttcggc ccacatcgac gagctggccc tggcccagcc ggccgagcag ggcatgggcc
     5581 cgggcaccgg caactggggc ctgctggagg ccgaggacga agccgcggcc caggccctga
     5641 tcgcccgggc ggaagactgg ctgcgagaac agggcatgac ccgcgtgctc gccccgctgt
     5701 cgatgtcggt ctgggaagag ccgggccagc tgaccaaggg ttttgaccat ccgccgaccg
     5761 tgatgatggg ccaccagccg cagcgttatc agcactatat cgaggcg
//
LOCUS       DASUDV010000228         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_426769,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000228 DASUDV010000000
VERSION     DASUDV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426769"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..736
                     /locus_tag="VFV30_04930"
     CDS             77..736
                     /locus_tag="VFV30_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX4847465.1"
                     /translation="MSRSGKDPGERLRTARKRSTSSARWLTRQLNDPYVKQAKAQGWR
                     SRAAFKLLELDEKYALIKGARRVVDLGIAPGGWAQVVRKRNPKAAVVGIDLLPTDPIE
                     GVTIFQMDFMADEAPAALTEALDGPPDLVISDMAANTVGHKQTDHLRTMGLVETAADF
                     AIANLAPGGAFVAKVLAGGTDTDLLALLKKHFATVKHAKPPASRKDSSEWYVIAQGFK
                     G"
     gene            complement(803..1099)
                     /locus_tag="VFV30_04935"
                     /pseudo
     CDS             complement(803..1099)
                     /locus_tag="VFV30_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
     gene            complement(1196..1678)
                     /locus_tag="VFV30_04940"
                     /pseudo
     CDS             complement(1196..1678)
                     /locus_tag="VFV30_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
     gene            1839..>2795
                     /locus_tag="VFV30_04945"
     CDS             1839..>2795
                     /locus_tag="VFV30_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4847466.1"
                     /translation="MVWKKEVEELRERRKLAEQMGGPEKVARQHARGKLDARARIKGL
                     VDPGSFREIGKIAGKGHYGPDGELLDLSPSNLVFGRANIEGRPVVASADDFTVRGGAA
                     DAAIHRKFVQCEQMASTLKIPLIRMIDGTGGGGSVKTLEEIGATYVPATPGWGEIVKN
                     LETVPVVALALGPTAGLGAARVVASHYSIMVKGLSQLFAAGPAVVEAIGEASDKESLG
                     GSIIHTRNGVVDEEVASEAEALATARRFLSYLPSHVGQHAPRMANTDPVDRKEQSLIS
                     LVPREAKQVYSMRKLLDAAFDKGSVFEMGRQWGRAAITAFARL"
BASE COUNT          459 a          933 c          963 g          440 t
ORIGIN      
        1 tgcgcagcct gatggccgcc ggcggcgccg gcgaacggac ccgcgcggcg ctgcggcaac
       61 tcaagcgggg tgaacgatga gccgttccgg caaggatccg ggggagcgcc tgcgcactgc
      121 ccgcaagcgc agcaccagct ctgcccgctg gctgacccgc cagctcaacg acccctatgt
      181 caagcaggcc aaggcccagg gctggcgcag ccgcgccgcc ttcaagctgc tggaactgga
      241 tgagaagtac gccctgatca aaggcgcccg ccgggtggtc gatctgggca ttgccccggg
      301 tggctgggcc caggtggtcc gcaagcgcaa ccccaaggcg gcggtggtgg ggatcgacct
      361 gctgccgacc gacccgatcg agggggtgac gatcttccag atggatttca tggcggacga
      421 ggcccccgcc gcgctgaccg aagcgctgga cggcccgccc gatctggtga tttccgacat
      481 ggccgccaac accgtgggcc acaagcagac cgatcacctg cgcaccatgg gcctggtcga
      541 aaccgccgcc gatttcgcga ttgcgaacct ggcccccggc ggcgcttttg tggccaaggt
      601 cctggccggc ggcaccgaca ccgatctgct ggccctgctg aaaaagcact tcgccacggt
      661 caagcacgcc aagcccccgg cgagccgcaa ggattcgtcg gaatggtacg tgatcgcgca
      721 gggcttcaag ggctgaagcg aaaagggcgg agccttgcgg ccccgccctt tcgttcgtcc
      781 cggaaggccg gtcttacttg accgacttca ggtaggcgat gatgttggcg cgttcctgcg
      841 catcggccag accggcgaag gtcatcttgt tgccgggcgc gaacttgctc ggatcggtca
      901 gccaggcgtc catggtcgca tcgtcccagt tgccgccctt ggtcttcagc gcatccgaga
      961 aggcatagcc gccgcggccc tgaccgattc cttcgccgaa cacgccggcc aggttcggac
     1021 cgatgccgtt ggcgccgccc gggttgatcg tgtggcaggc cgcgcacttg gcgaagctct
     1081 tctggccctt ggcggcatcg ccgaccacgg cggccgcttc gggcgcggca ccggcagcgg
     1141 gagcttccgg cacggccggg agcggcagcg ggctggcgct cttcgaattg aggtagacca
     1201 tcaggttggc gcgttcctgc gggtccgtca ggccggcgaa gctcatcttc gtgcccgggg
     1261 catacttggc cgggttttca agccaggcgc tcatgcttgc ccagtcccac ttgccgccct
     1321 tgcccttgag ggcttcggag taggagaacg gatggctcgc cacattggcg cccagcgtgc
     1381 cgtagagatt cgggccaaga ccggccgcgc cgccctgctc gatcgtgtgg cagctggcgc
     1441 acttggcaaa ggtcttctcg cccttggcga tgtcggcctt ggccagatag gcttcgaacg
     1501 gggtggcgtc cttgccgcct tcgccttcct ggacgactcc ggcaatctca tagcccatct
     1561 tctcgggccg ctgttccttg ccggcgtgga aataacgcgt gctgacggcc gaaaggccga
     1621 gcaggacgat gccggcaccc aaggtccatc cggcgaaggt attgaagcga tcagacatgg
     1681 atagtgcccc gttgggttgt gcctgtgact tttcggggcc gctctagtag cgcgccgcgc
     1741 cgcgcgcaag gggcattgca gccctgccaa ttgcgcccta gccactgtcc ccaagaaaac
     1801 aggaccgccg cgccagcgcg gccggggtgg aggcggattt ggtctggaaa aaggaagtcg
     1861 aggaactgcg cgaacggcgc aagctggccg agcagatggg cgggccggaa aaggtcgcgc
     1921 gccagcacgc ccgcggcaag ctggacgccc gggcgcggat caagggcctg gtcgatcccg
     1981 gctcgttccg cgaaatcggc aagatcgccg gcaagggcca ttacgggccc gacggcgagc
     2041 tgctcgacct ctccccctcc aacctggtct tcggccgggc caatatcgaa ggccgcccgg
     2101 tggtggccag cgccgacgat ttcaccgtcc gcggcggagc ggccgacgcg gcgatccacc
     2161 gcaagttcgt gcagtgcgag caaatggcga gcacgctgaa gatcccgctg atccggatga
     2221 tcgacggcac cggcggcggc ggatcggtca agacgctgga ggaaatcggc gcgacctatg
     2281 tgcccgccac gccgggctgg ggcgagatcg tcaagaacct ggagacggtg ccggtcgtcg
     2341 ccctcgcgct gggccccacg gcaggcctgg gcgcggcgcg ggtggtggcc agccactatt
     2401 cgatcatggt caagggcctg tcccagctgt tcgccgccgg ccccgcggtg gtcgaggcga
     2461 tcggggaggc gagcgacaag gaatcgctgg gcggatcgat catccacacc cgcaacggcg
     2521 tggtcgacga ggaagtggcg agcgaggccg aagccttagc cacggcgcgg cgcttcctgt
     2581 cctacctgcc cagtcacgtt ggccagcacg ccccgcgcat ggccaatacc gacccggtgg
     2641 accgcaagga gcaaagcctg atcagcctgg tcccgcgtga ggccaagcag gtctattcga
     2701 tgcgcaagct gctggatgcc gccttcgaca agggctcggt ctttgaaatg ggccgccagt
     2761 ggggccgcgc cgcaatcacc gcctttgccc ggctg
//
LOCUS       DASUDV010000229         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1884736,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000229 DASUDV010000000
VERSION     DASUDV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884736"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VFV30_04950"
     CDS             <1..114
                     /locus_tag="VFV30_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX4847467.1"
                     /translation="PMARALRGAAVGDLRKVHLPGGEKEWEVIAIRYPESA"
     gene            117..296
                     /locus_tag="VFV30_04955"
     CDS             117..296
                     /locus_tag="VFV30_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEX4847468.1"
                     /translation="MAEVINLRLARKAKARASAAQQAAENRVRHGRSKTERQIQQDEA
                     DRTARVLDGAKRDDS"
     gene            296..499
                     /locus_tag="VFV30_04960"
     CDS             296..499
                     /locus_tag="VFV30_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847469.1"
                     /translation="MRTTYDGATVRLYHLDSASEGGAATTLLYGPLAEALRLAEQQPA
                     ELQEGLFIATDNDVVAYLDLIGE"
     gene            553..1080
                     /locus_tag="VFV30_04965"
     CDS             553..1080
                     /locus_tag="VFV30_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847470.1"
                     /translation="MVSASRPRSLRKPAWIAAGLLGLGLIASPVLARESLGMFGGWAA
                     FVDRQAPRCYAIAKAAPSTMQRDYQPYMSVAWWPRAQVRGQVHFRLSRKLQPGAPITV
                     SVGGQKIPLVGGGGDAWAADKRGDAAIMAAMRSSGELTVSARDANGRGFSNTYSLSGA
                     ATAMDAAAVGCAQLR"
     gene            1116..2378
                     /gene="rlmN"
                     /locus_tag="VFV30_04970"
     CDS             1116..2378
                     /gene="rlmN"
                     /locus_tag="VFV30_04970"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX4847471.1"
                     /translation="MADTALMPIPGQIDPVPTPRDVTPREDGRVDLIGLPKKRITELF
                     EAAGLDAKAAKLRAKQVFHWLYHRGVTDFEAMTDIAKTMRPWLAERFVIGRPDIVLAQ
                     HSSDGTRKWLLRTADGHEFEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNL
                     TPGEIVGQVMLARDALGEWPRAGQADLAALGDDLVDEDGGYSSDGRLLTNIVMMGMGE
                     PLYNFDNVRDALKLVMDGDGLALSKRRITLSTSGVVPMMAKCGEEIGVNLAVSLHAVS
                     KDVRDEIVPINRKYGLEELLQACADYPGASNARRITFEYVMLKDKNDSDEDARELVRL
                     IKQYKLPAKVNLIPFNPWPGAIYECSSPERIRRFSEIVFEAGISAPVRTPRGRDIDAA
                     CGQLKTAAKKMSRAELDRLAEEKQAALG"
     gene            2437..2703
                     /locus_tag="VFV30_04975"
     CDS             2437..2703
                     /locus_tag="VFV30_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847472.1"
                     /translation="MKRSIFVLAAAALALGGCSSEPKPDDAAAKTAEKSEGKKAEKGK
                     DKKDSKGKKAAADKPHYNPWAKDQPAAAAGAEVADAGEGAKKDS"
     gene            2799..3152
                     /locus_tag="VFV30_04980"
     CDS             2799..3152
                     /locus_tag="VFV30_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX4847473.1"
                     /translation="MKKALTASVIAASLLSAAAPAMADPPRWAPAHGWRDHHGDVRPK
                     RTSNGIQYWRGEDGRYYCRRGDGTVGLLVGAALGALIGRAVDNRGERATGTILGAAAG
                     AFIGNELAKGPTRCR"
     gene            3230..>3743
                     /locus_tag="VFV30_04985"
     CDS             3230..>3743
                     /locus_tag="VFV30_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847474.1"
                     /translation="MARPAVLITGAAKRIGAVLARRFAAAGWHVVVHAGHSPALACDF
                     AATLPSAEVVEADLVDREAALAMIADLAARLEDWRVLVNSAAVFKPDTAAGIDPAIFA
                     EAVRVNAETPTAMAQAFLASARTKGGKRVIQFLDQKLLNPNPDFFSYTMAKHAFASTV
                     KMLAMAQDDPA"
BASE COUNT          632 a         1315 c         1239 g          557 t
ORIGIN      
        1 ccgatggccc gggccctgcg cggcgcggcg gtgggggatc tgcgcaaggt ccacctgcca
       61 ggcggcgaga aggaatggga agtgatcgcg atccgctatc cggagagtgc ctgagcatgg
      121 ccgaagtcat caacctgcgc ctcgcccgca aggccaaggc gcgcgcctcg gcggcccagc
      181 aagcggccga gaaccgcgtc cgccacggcc gcagcaagac cgagcggcag atccagcagg
      241 acgaggccga ccgcaccgcc agggtgctcg acggcgccaa acgggacgac agctgatgcg
      301 caccacctat gacggggcca cggtccggct ctaccacctc gatagcgcca gcgaaggcgg
      361 cgcggccacc accctgctct acggcccgct ggccgaagcg ctgcggctgg ccgaacagca
      421 accggccgag ctgcaggaag gcctgttcat cgccaccgac aacgatgtgg tcgcctacct
      481 cgacctgatc ggggaatagc ggcggcgcgg ctgccctttc cctgtccgcc gctttctgca
      541 atgaggccgg ccatggtttc cgcttcccgc cctcgttcgc tgcgcaagcc ggcctggatc
      601 gcggccggcc tgctgggcct gggcctgatc gccagcccgg tgctggctcg ggagagcctg
      661 ggcatgttcg gcggctgggc ggctttcgtc gatcgccagg caccgcgctg ctatgccatc
      721 gccaaggcgg cgccatcgac catgcagcgc gactatcagc cctatatgtc ggtcgcctgg
      781 tggccgcgcg cacaggttcg cgggcaggtg cacttccgcc tttcgcgcaa gctgcagccc
      841 ggcgcgccga tcacggtttc ggttggcggg cagaaaatcc cgctggtcgg cggcggcggc
      901 gatgcctggg cggccgacaa gcgcggcgac gcggcgatca tggcggcgat gcgatcgtcc
      961 ggtgaattga ccgtcagcgc ccgcgatgcg aacggccgcg gcttctccaa cacctattcg
     1021 ctgagcggcg ccgccaccgc gatggatgcc gccgcggtcg gctgcgcgca gctgcgctag
     1081 cttttccggc ccgatcgccc tataggcccg cgcctatggc cgataccgcc ctcatgccga
     1141 tcccgggaca gatcgatccc gtccccaccc cgcgcgacgt gaccccgcgc gaggatggtc
     1201 gcgttgacct tatcggcctg ccgaaaaagc ggatcaccga actgttcgaa gccgccgggc
     1261 tggatgccaa ggccgccaag ctgcgcgcca agcaggtgtt ccactggctc taccaccgcg
     1321 gcgtcaccga tttcgaggcg atgaccgaca tcgccaagac catgcgcccg tggctggccg
     1381 aacggttcgt gatcggccgc ccggacatcg tcctggccca gcattccagc gacggcaccc
     1441 gcaagtggct gctgcggacc gcggacggtc acgagttcga gatggtcttt atccccgatg
     1501 cggaccgcgg cacgctgtgc gtttccagcc aggtcggctg cacgctcaat tgccggttct
     1561 gccacaccgg cacgatgcgg ctggtgcgca acctgacccc gggcgagatt gtcggccagg
     1621 tcatgctggc gcgcgacgcg ctgggcgaat ggccgcgcgc cgggcaggcg gatctggccg
     1681 cgctgggcga cgatctggtc gatgaggacg gcggctattc gtccgacggg cgcctgctga
     1741 ccaacatcgt gatgatgggc atgggcgagc cgctctacaa tttcgacaac gtccgcgatg
     1801 cgctgaagct ggtgatggac ggcgatggcc tggccctttc gaagcgccgg atcacccttt
     1861 cgaccagcgg cgtggtgccg atgatggcca agtgcggcga ggagatcggc gtcaacctgg
     1921 ccgtttcgct ccacgccgtg tccaaggacg tgcgcgacga gatcgtgccg atcaatcgca
     1981 agtacggcct cgaagagctg ctgcaggcct gcgccgacta tcccggtgcc agcaacgccc
     2041 ggcgcatcac gttcgaatac gtgatgctga aggacaagaa cgacagcgac gaggacgcgc
     2101 gcgagctggt tcgcctgatc aagcagtaca agctgccggc caaggtcaac ctgatcccgt
     2161 tcaacccctg gcccggcgcg atctacgaat gctccagccc tgagcggatc cgccgctttt
     2221 ccgagatcgt cttcgaagcc gggatcagcg ccccggtgcg caccccgcgc gggcgcgaca
     2281 tcgatgccgc ctgcggccag ctcaagaccg cggccaagaa gatgagccgg gcggagctgg
     2341 accggctggc cgaagaaaag caggccgcgc tcggctgacg cttaccgcct gttaggaccc
     2401 tgccgctatg ggggagcctt caggaggttc ttaaccatga aacggtctat tttcgttctc
     2461 gccgctgctg ccctcgcttt ggggggctgt tccagcgaac ccaaacccga tgatgccgct
     2521 gccaagacgg ccgagaaatc ggaaggcaag aaggccgaaa agggcaagga caagaaggat
     2581 tccaagggca agaaggcggc tgccgacaag ccgcactaca acccctgggc caaggaccag
     2641 cccgccgctg cggccggtgc cgaagtcgcc gacgccggcg aaggcgccaa gaaagacagc
     2701 tgatcgcgct ttcggcacgg ctcgaccgtg ccggaaatct gcggttcatt tagccgggcc
     2761 cagcctgctc ccgtgacgaa aacaacggga gcacgatcat gaaaaaggcc cttaccgctt
     2821 cggtgattgc cgccagcctg ctgtctgccg ccgcacccgc catggccgat ccgccgcgct
     2881 gggctccggc ccacggctgg cgcgatcacc acggcgatgt ccgccccaag cggaccagca
     2941 acggcatcca gtactggcgc ggtgaagacg gccgctacta ctgccgccgc ggcgacggga
     3001 ccgttggcct gctggtcggt gccgcgctcg gcgcgctgat cggccgtgcg gtcgacaacc
     3061 gcggcgaacg cgccacaggc acgatcctgg gcgccgccgc cggggccttc atcggcaacg
     3121 agctggccaa ggggccgacc cgctgccgct gaacccggcg ctgaaccgcc cggaacctga
     3181 acctctgggc ggttgacctg gcgggccatc ccccgcaaag ccccaaggga tggcccgacc
     3241 cgcagtcctg attaccggcg ccgccaagcg cattggtgcc gttctggccc gccgctttgc
     3301 cgcggccggc tggcacgtgg tggtccacgc cggccattcc cccgcccttg cctgcgattt
     3361 cgccgccacc ctgccctcgg ccgaagtggt cgaggccgat ctggtggacc gcgaggccgc
     3421 attggcgatg atcgccgatc tggccgcccg gctggaggac tggcgggtgc tggtcaacag
     3481 cgcggcggtg ttcaagccgg acactgccgc gggaatcgac ccagcgatct ttgccgaggc
     3541 cgtgcgggtc aacgccgaaa cccccaccgc catggcccag gccttcctcg cctcggcccg
     3601 gaccaagggc ggcaagcggg tgatccagtt cctcgatcag aagctgctca accccaatcc
     3661 cgatttcttc agctacacca tggccaagca cgcctttgcc agcacggtga agatgctggc
     3721 catggcccag gacgatccgg cca
//
LOCUS       DASUDV010000230         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_912982,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000230 DASUDV010000000
VERSION     DASUDV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912982"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2392)
                     /locus_tag="VFV30_04990"
     CDS             complement(<1..2392)
                     /locus_tag="VFV30_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX4847475.1"
                     /translation="MTSKPALAAILAKRLDGSLLPGDAPFASGGLDAAAAFVLAAAET
                     RGAGETAIAIDSVSGAAGERFMRVALINDDMPFLVDSIAACVAAHGLGIDRLLHPVLA
                     VRRDGARKLEAIVEGDAAGEKRESMIYLETERADAKLRRQLETSLASTLADVRAAVAD
                     WPRMQEAMGSDADSLADGEGAALLRWFKDGMLTQLGHVVRRRDGSQSEALGICRTSAK
                     ALLSPASFERAFAWFEGAGKAGHVPLIIKSNLIAQVHRRVPLDLFIVPRFEGGKLAAL
                     SIHAGVWTSAALAAAPDRVPRMRAQLAELMSRFGFNPQAHAGKALVHALTALPHDILI
                     GFSDADLTRIATAMMSLVDRPRPRLALVRAPLSRHLYAFVWLPRDSMSTTIRLQIADM
                     LSKAAEAPVLDWSLQIEGSSLAMLRFVLDIRDGAEAPDEAALEARLQAMVRGWADAVE
                     AEIAASEEPSRAAAIAARFAEAFPVSYRTAYGPAEAATDIARLRRLAAEGEEGPRRGA
                     RLLAGEGANELRLKLYQRSGAIALSDAVPALENFGFKVIEDLPTDLDEGRAGTIHDFA
                     LALPAAQAAADLLARADVLEAAVADVLNGQAEDDPFNRLIAAASLSAQEANWLRAWYR
                     YLRQAGMNFGIPTVVDALQHAPAVTRGLIALFVTRHDPGFKGDRAKAESAAQDQIREG
                     LARVSAINDDRLLRQYRALVEAVLRTNAFAPASAEALAFKLDSAAVPGLPKPVPYREI
                     FVYSRRVEGIHLRSGPIARGGLRWSDRRDDFRTEILGLMKAQRVKNAVIVPTGAKGG"
     gene            complement(2524..>2733)
                     /locus_tag="VFV30_04995"
     CDS             complement(2524..>2733)
                     /locus_tag="VFV30_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX4847476.1"
                     /translation="NYRASLPPAALAMLEHMRSVSAVGSPETVASALARVIERTGADE
                     IIVSGATFDPAARRRSLELTAKILT"
BASE COUNT          457 a          920 c          944 g          412 t
ORIGIN      
        1 acccgccctt ggccccggtc ggcacgatca ccgcgttctt gacccgctgg gccttcatca
       61 gcccgaggat ttcggtgcgg aagtcgtcgc gccggtccga ccagcgcagg ccgccgcggg
      121 cgatcgggcc cgagcgaagg tggatcccct cgacccggcg tgaatagacg aagatctcgc
      181 ggtagggcac cggcttgggc aggcccggaa ccgcggcgga atcaagcttg aaggccaacg
      241 cttcggcgct tgccggggca aaggcattgg tccgcagcac cgcttcgacc agcgcgcggt
      301 actggcgcag cagccgatcg tcgttgatcg cgctgaccct ggccagccct tcgcggatct
      361 ggtcctgcgc ggcactttcg gccttggccc gatcgccctt gaaacccgga tcgtggcggg
      421 taacgaacag cgcgatcagc ccgcgggtga cggcgggcgc atgctggagc gcatcgacca
      481 cggtcgggat gccgaaattc atccccgcct ggcgcagata gcgataccag gcgcgcagcc
      541 agttggcttc ctgggcggag agcgaggcgg cggcgatcag ccggttgaac ggatcgtctt
      601 cggcctgacc attgagcacg tcggccacgg ccgcctcgag cacgtcggcg cgggccagca
      661 ggtcggcggc ggcctgggca gcgggcagcg ccagcgcgaa atcgtggatc gtccctgccc
      721 ggccctcgtc gagatcggta ggcaggtcct cgatcacctt gaagccgaag ttttcaagcg
      781 cgggcaccgc atcagacaac gcaatcgcgc cgctgcgctg atagagcttg aggcgcaatt
      841 cattcgcgcc ctctccagcc agcagccggg caccgcggcg cgggccttcc tcaccctcgg
      901 cggccaggcg tcgcagccgg gcgatgtcgg tcgcggcctc ggccgggcca taggcggtgc
      961 gataggaaac cggaaaggct tcggcaaagc gggcagcgat cgcggcggcg cgcgatggtt
     1021 cctcgctcgc ggcgatttcc gcctcgaccg catcggccca gccgcgaacc atggcctgca
     1081 ggcgggcttc cagcgccgct tcatcgggcg cttcggcgcc atcgcggatg tcgagcacga
     1141 agcgcaacat cgccaggctc gaaccctcga tctgcaggct ccagtcgagc accggcgcct
     1201 cggcggcctt ggacagcatg tcggcgatct gcagccggat cgtggtcgac atcgaatcgc
     1261 gcggcagcca gacgaatgca tagaggtggc gcgaaagcgg ggcacgaacc agcgccaggc
     1321 gcgggcgcgg gcgatcgacc aggctcatca tcgctgtggc gatgcgggtc aggtcggcat
     1381 cggaaaagcc gatcaggata tcgtgcggca gcgcggtcag cgcatggacc agcgccttgc
     1441 cggcgtgggc ctggggattg aagccgaacc ggctcatcag ttcggccagc tgcgcgcgca
     1501 tccgcggcac ccgatcgggc gcagcagcca gcgcggcgct ggtccagacc ccggcatgga
     1561 tcgacagcgc ggcaagcttg ccaccttcga agcgggggac gatgaacagg tcgagcggaa
     1621 cccggcggtg aacctgggcg atcaggttgg acttgatgat cagcggaacg tggcccgcct
     1681 tgccggcccc ctcgaaccag gcaaaggccc gttcgaacga ggcgggagag agcagcgcct
     1741 tggcgctggt gcggcagatc cccagcgctt ccgactggct gccatcgcgg cggcgcacga
     1801 catggcccag ctgggtcagc atgccgtcct tgaaccagcg cagcagcgcc gcgccttcgc
     1861 catcggcgag gctatcggcg tccgaaccca tggcttcctg catgcgcggc caatcggcca
     1921 ccgcagcgcg gacatcggcg agcgtgctgg cgagcgaggt ttcaagctgg cggcgcagct
     1981 tggcatcggc gcgctcggtc tcgagataga tcatcgattc gcgcttttcg ccggcggcat
     2041 cgccctcgac gattgcctca agcttgcgcg cgccatcgcg gcgcaccgcc agcacggggt
     2101 gcagcagccg atcgatcccc aggccatgcg cggcaacgca ggccgcgatc gaatcgacca
     2161 ggaacggcat atcatcgttg atcagcgcca cgcgcatgaa acgctcgccg gccgcgcccg
     2221 aaaccgaatc gatcgcaatc gcggtttcgc ccgcgccgcg ggtttcggcc gcggccagga
     2281 caaaggccgc tgccgcatcg agcccgccgc tcgcaaaggg cgcatcgccc ggcaggagcg
     2341 agccgtccag gcgtttggcc aggatcgcgg caagggcggg cttcgatgtc attgcaatgc
     2401 tcctcaggcg gcgttggcca cccgttcctg cttgttatat tattgctgcc gtcggcagca
     2461 tttgttccga ttgtagaggc aactggtcac cactgcaacc accttgccgc tagggaaatg
     2521 ggcctaagtg agtatttttg ccgttaattc gagcgagcgg cgccgcgccg ctggatcgaa
     2581 ggtcgcgccc gaaacgatga tctcgtcggc gccggtgcgc tcgatcaccc gggcaagggc
     2641 gctggccaca gtctcgggcg aacccaccgc gctgactgaa cgcatgtgtt cgagcatggc
     2701 cagcgcggcg ggcggcaggc tggcgcgata gtt
//
LOCUS       DASUDV010000231         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1266180,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000231 DASUDV010000000
VERSION     DASUDV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266180"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="VFV30_05000"
     CDS             <1..1663
                     /locus_tag="VFV30_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HEX4847477.1"
                     /translation="AKVQEGLDVIDPEGRWQVRASGAATGTVHGRGCTLGIDFSAVPM
                     PAYLPELALWRSFWVRSGEPLRALRRHGAYVTIGCDLDCEAAEFVDIRQSAKAMCLAL
                     AVIAKFGPCVGVMNAAQNCVYTEDQLDMLVGPLGSDEVSIKLFLWTAFHSTSDDAVSL
                     STAGLLPFVGREVECWNAPGTLDFVGNKLNGVLRYLLINGPVIRNGDTIGEVEGDRSI
                     RIYHGESDDRSARPHSVPVLYMEFEGPGGASAPRPDPAVPALDADPQIQLMLEALRAD
                     ASPQSRAMLDELEELIANPPPREVIESDANPIARLLMRWIALEQGEGKLPGGQDGPWQ
                     PPLDSVGALALIALTQGIGYPREQLEEMLGEAFPTYTWKAEEGPGGLNNPKAVLGQMP
                     GRTIGVWIEARKEALPAAIAAPPHRVHVRVAVDAGRDAALARRVALVVASTLFLFQDK
                     AGHVQLGPDGNWLAHDDALTGVILPRHTTDISGFEQASGIPVVKLRGGNWMDGAAPPS
                     NDRLEEVYFAQAPAAAPSPPVQPAAPGALGFGRRAAGGGFGRKGL"
     gene            complement(1660..>2069)
                     /locus_tag="VFV30_05005"
     CDS             complement(1660..>2069)
                     /locus_tag="VFV30_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX4847478.1"
                     /translation="PAARDPGRSVLTVRNPESPFWIGNLKNSEFRCLVPASGLMEWGK
                     GHDAEGKRRRHWLGCADQPLFALAGVWKDSEVPGFALLTTEPNAHLRALGREAMPVIL
                     PPDPAAWQVWLCGSWDRAARLIAPYSSSLMTEI"
BASE COUNT          322 a          684 c          733 g          330 t
ORIGIN      
        1 ggccaaggtc caggaagggc ttgacgtgat cgaccccgaa gggcgctggc aggtgcgtgc
       61 cagcggggcc gcaacgggca cggtccacgg ccgcggctgc acgcttggaa tcgatttcag
      121 cgcggtgccg atgcccgcct acctgcccga actggcgctg tggcgcagtt tctgggtgcg
      181 ttcgggcgaa ccgctgcgcg cgctgcgccg gcacggcgcc tatgtcacga tcggctgcga
      241 cctcgattgc gaggcggccg aattcgtcga catccggcaa agtgccaagg caatgtgcct
      301 ggccctggcg gtgatcgcca agttcggccc ctgcgtaggg gtgatgaacg cggcgcagaa
      361 ctgcgtctat accgaagatc agctcgacat gctggtcggc ccgctgggca gcgacgaggt
      421 ttcgatcaag ctgttcctgt ggaccgcgtt ccattcgacc agcgacgatg cggtttcgct
      481 gtccacggcg gggctgctgc ctttcgtcgg gcgcgaggtc gaatgctgga acgcgccggg
      541 cacactcgac ttcgtgggca acaagctgaa cggggtgctg cgctatctgt tgatcaacgg
      601 cccggtgatc cgcaacggcg ataccatcgg cgaggttgag ggcgatcgct cgatccggat
      661 ctaccacggc gaaagcgatg atcgctcggc ccgtccgcat tcggtgccgg tgctctatat
      721 ggaattcgaa ggtccgggcg gggccagcgc accgcggccc gatccggcgg tgccggcgct
      781 cgatgccgat ccgcagatcc agctgatgct cgaggcgctg cgcgccgatg cttcgcccca
      841 gtcgcgcgcc atgctcgacg agctggaaga gctgatcgcc aacccgccgc cgcgcgaagt
      901 gatcgaaagc gatgccaatc cgatcgcccg gctgctgatg cgctggatcg cgctcgaaca
      961 gggcgagggc aagctgccgg gcggtcagga cgggccctgg cagccgccgc tcgactcggt
     1021 cggcgcgctg gcgctgatcg cactcaccca ggggattggc tatccgcgcg aacagcttga
     1081 agaaatgctg ggcgaagctt tcccgactta tacctggaag gccgaagaag gcccaggcgg
     1141 gctcaacaac cccaaggcgg tgctgggcca aatgcccggg cggacaatcg gggtgtggat
     1201 cgaggcgcgc aaggaagcat tgcccgccgc gattgccgcc ccgccgcacc gggtccacgt
     1261 gcgcgttgcg gttgatgccg ggcgcgatgc ggcgctggcc cggcgcgttg cgctggtggt
     1321 ggcatcgacg ttgttcctgt tccaggacaa ggcaggtcac gtccagctcg gcccggatgg
     1381 caactggctg gcgcacgacg atgcgctgac cggcgtgatc ctgccgcgcc acaccaccga
     1441 catatcgggc ttcgagcagg ccagcggcat tccggtggtc aagctgcgcg gcggtaactg
     1501 gatggatggc gccgcgccgc cttcgaacga ccggcttgaa gaagtttact tcgcccaggc
     1561 ccctgcggca gcaccgtcgc cgcccgtgca gcccgcagcg cccggtgcac tgggcttcgg
     1621 acgccgtgct gccggcggcg ggttcgggcg caaggggctc tagatctcgg tcatcaggct
     1681 ggagctgtag ggcgcgatca gcctggccgc ccggtcccag ctgccgcaca gccagacctg
     1741 ccaagccgcc ggatcgggcg gcaggatcac cggcatggcc tcgcgcccca gcgcgcgcag
     1801 gtgggcgttg ggctcggtgg taagcagcgc gaagcctggc acctcgctgt ccttccacac
     1861 cccggccagc gcgaacagcg gctgatcggc gcagcccagc cagtggcgtc ggcgcttgcc
     1921 ctcggcgtcg tgacccttgc cccattccat caagccactg gccgggacca ggcagcggaa
     1981 ttcgctgttc ttgaggttgc cgatccagaa cgggctttcg ggattgcgca cggtcagcac
     2041 gctgcggccg ggatcgcgcg cggcgggcg
//
LOCUS       DASUDV010000232         6277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1553625,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000232 DASUDV010000000
VERSION     DASUDV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553625"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFV30_05010"
     CDS             complement(<1..281)
                     /locus_tag="VFV30_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847479.1"
                     /translation="MSEPAGHPHHEPTAKEMRLVIGASSAGTVFEWYDFFIYGALGAI
                     LSKTFFPTGNATLEMLLFWLVFAVGFGFRPLGAILFGYLGDKLGRKYTFL"
     gene            complement(333..752)
                     /locus_tag="VFV30_05015"
     CDS             complement(333..752)
                     /locus_tag="VFV30_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX4847480.1"
                     /translation="MSSAAALYTPEVLALATGLAGYPLSDGLALRGSARAPLCGSTIA
                     IGLALDADERIAGLGIKAHACAIGQAAAGIFAAAARGQDRAGIAKALAEVELWLTDEG
                     AGLPDWPGLSAIAAARAYPARHGAILLPWKAALSALP"
     gene            complement(749..1306)
                     /locus_tag="VFV30_05020"
     CDS             complement(749..1306)
                     /locus_tag="VFV30_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX4847481.1"
                     /translation="MTGFDIAVLVLVGLGAITGFMRGFVQEVFALAAWVLAIVALRTL
                     HTPVSEGLMPYIGTETGSAVLAFSLLLLVPYAIVKLLSDRLGSASRNSVLGPIDRVLG
                     FGFGAIKGMIIVVLAFSVLALGYDTVWGAKGRPDWITLSRSYPFVNAGSKYVIQLIAE
                     RHKEAAKAEAEEIGKEAIKTKLSGK"
     gene            complement(1319..2686)
                     /gene="radA"
                     /locus_tag="VFV30_05025"
     CDS             complement(1319..2686)
                     /gene="radA"
                     /locus_tag="VFV30_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX4847482.1"
                     /translation="MAKPKRRYICQACGSVSHRWQGQCADCGEWNTLAEEAPATVFSA
                     KHDLSSGGRAIAFEALDAPGEVLKRKSTGLAEFDRALGGGLVPGSAVLMGGDPGIGKS
                     TLLLQAAARIAQSGASAVYISGEEAAGQVRMRADRLGLAGAPISLAAATSVRDILTTL
                     ATMPPPALLVIDSIQTMHSDQIEGAPGTVSQVRASAFELIRYAKENGVALFLVGHVTK
                     DGSIAGPRVLEHMVDVVMSFEGERSHQYRILRCIKNRFGPVDEIGVFAMEGAGLAEVG
                     NPSMLFLSGRETPVAGSAVFPAIEGTRPVLIEVQALIVRLQSGATPRRAVVGWDSGRM
                     AMLLAVLEARCGLNFSSAEVYLNVAGGYRLADPAADLAVAAALVSALADKPLPTEAVW
                     FGEVSLAGEIRPVAHAAIRLREAAKLGFGQAFGPGEGTGGAEGSRFTRLAMLPNLVDR
                     IMGGA"
     gene            complement(2730..3233)
                     /locus_tag="VFV30_05030"
     CDS             complement(2730..3233)
                     /locus_tag="VFV30_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847483.1"
                     /translation="MFADVNIEMLINPSVQPVVMATATRRKSRPAGAGLSSVILQCTG
                     TSQIRSNDGTVTSRDTQDIRVNLDFRNKTGQVFGVVPTLGGELIFKEPQPLTITNGIA
                     SFQSAPLGPERTTAPLQIDLNSGAMSIRNRHLLGRAFGAIPLYNTFEMAGYCKPLNQR
                     LPIVPRT"
     gene            3331..5658
                     /locus_tag="VFV30_05035"
     CDS             3331..5658
                     /locus_tag="VFV30_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HEX4847484.1"
                     /translation="MRQKELRIALVCYGGVSLAVYMHGVTKELWKLARASRAFHGGAA
                     ISGGTEAVYVRLLERIEKHFKLRLRVLPDIVAGASAGGINGVFLAQAIHSGQSLEPLT
                     ELWLERADVDVLLDPDARPGSRIAKFWAMPIVYWLLKRPGNVVSSSVAPETRSEVRAK
                     LSRLIRSRWFEPPFGGLGFSRLLCEALGTMKAGGADAPLLPAGHPIDLFVTATDFRGH
                     AQVLRLHSPEQIEESEHRLSIGFRSPTPAEGGGALAAPIELVFAARATASFPGAFPAL
                     QLAEIDSLAREHGDPWHSRTAFIERIMPEHSHRGDAEQVALIDGSVLVNAPFAEAMAV
                     LRDRPAQREVDRRFVYIDPHPDEVGGQRHSETGPPGFFQVIFGSLSSIPREQPVRDNL
                     EAIARQSRQLAGLKAIVDSLRPDVEEAVERLFGRTLFLDRPTVKRLSAWRAKAQQAAA
                     ERAGFTFQAYAQVKLAGIVQSLAATIQQAAPELGMAGDEAVADRLSAWLATHGLDHLT
                     ASSGGGSAEAVAFFREHDLGFRIRRLRLLARRLSENWDDIDAQAPEAREAARTVVYKA
                     LSLYFERDASGSLGRDFPDTARRVLHHPGEVLSAIAQRRQLPATDLVVDAMLVECLAT
                     LPRELKRRILLTYLGFPFYDTVTLPLLRGEGQTEFEPAKVDRISPDDCNSIRGGGASA
                     VLRGTEFYNFGAFFSRAYRENDYLWGRLHGAERMIDLIASALPTGMVLDPAELARFKR
                     DAFLAVLDEEEGRLKADPGLVAGVRAEVLGEHESI"
     gene            5645..6022
                     /locus_tag="VFV30_05040"
     CDS             5645..6022
                     /locus_tag="VFV30_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847485.1"
                     /translation="MNQSDRMNSASNGWEADPWDASDAVADLQLEGFQIRAAKAVSWS
                     AISLVEGPIFGHDIEWLNSLEVEYVATHDLEDWLLMRLVYHGFPDPPEWRLATRAAGK
                     FSHPWNSWGYFAQLPDAWKIPGI"
     gene            complement(6074..>6277)
                     /locus_tag="VFV30_05045"
     CDS             complement(6074..>6277)
                     /locus_tag="VFV30_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413517.1"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX4847486.1"
                     /translation="LRKRGSGMPVLQGRGRGDLVIEIMVETPTKLTSRQKELLRELQA
                     TETGEETPQSKGFFDRIKEAFGG"
BASE COUNT         1060 a         2138 c         2027 g         1052 t
ORIGIN      
        1 aggaaggtgt acttgcgccc cagcttgtca cccaggtatc cgaacaggat cgcgccgagc
       61 gggcggaagc caaagccgac cgcgaagacc agccagaaca gcagcatttc cagcgtggcg
      121 ttgccggtgg ggaagaaggt cttcgacagg atcgcgccga gcgcgccgta aatgaagaaa
      181 tcgtaccatt cgaacacggt gccggccgag gacgcgccga tgaccaggcg catttccttg
      241 gcggtgggtt cgtggtgcgg gtgcccggca ggctcgctca tgttcggctt ctccccatgg
      301 acttttgccg gtctctagcc cggcgcaagc gcttagggaa gcgcactcag cgccgccttc
      361 cacggcaaca ggattgcccc gtgccgggcc ggataggcgc gcgccgcggc aatggcggaa
      421 aggcccggcc aatccggcag gccggcgcct tcgtcggtca gccagagctc cacttcggcc
      481 agcgccttgg cgatcccggc gcggtcctgt ccgcgcgccg cggccgcgaa gattccggcc
      541 gcggcctggc cgatggcgca ggcatgggcc ttgatcccca gccccgcgat ccgctcatcc
      601 gcatcgagtg ccaggccaat cgcgatggtc gagccgcaca gcggcgcccg cgccgatccg
      661 cgcagggcaa gcccgtccga cagcggatag cccgcgagac ccgtcgccag ggcgaggacc
      721 tctggcgtgt agagcgcggc agcgctgctc acttgcccga aagcttggtc ttgatggctt
      781 ccttgccgat ctcttcggct tcggccttgg cggcttcctt atgccgctcg gcgatcagct
      841 ggattacgta tttgctgccg gcgttgacga agggatagga ccgcgaaagc gtgatccagt
      901 ccggccggcc ctttgcgccc cagaccgtgt catagccgag cgccaggacc gagaaggcca
      961 gcacgacaat gatcatcccc ttgatcgcgc caaagccgaa gcccagcacc cggtcgatcg
     1021 ggcccaggac ggaattgcgg ctggccgatc cgagccggtc cgagagcagc ttgacgatcg
     1081 cataggggac cagcagcagc agcgagaagg ccagcacggc ggatccggtt tcggtgccga
     1141 tgtagggcat cagcccttcg ctcaccggag tgtgcagggt gcgtagcgca acgatggcca
     1201 gaacccaggc cgccagggcg aacacctctt gcacgaagcc gcgcatgaac ccggtgatgg
     1261 ccccaagccc caccagaacg agaactgcga tatcgaaacc ggtcatggtt tgggaaagct
     1321 acgctccgcc catgatccgg tcaacgagat ttggcagcat ggcgagccgg gtgaagcggc
     1381 ttccttcggc cccgccggtg ccctcacccg ggccaaaggc ctgcccgaag cccagcttgg
     1441 ccgcttcgcg caggcggatc gcggcgtggg cgaccgggcg gatttccccg gcgagcgaga
     1501 cctctccgaa ccagaccgcc tcggtcggca gcggcttgtc cgccagggcc gagaccagcg
     1561 ccgcggcgac cgccagatcg gccgcgggat cggccaggcg atagccgccc gcgacgttga
     1621 gatagacttc ggccgaggag aagttgagcc cgcagcgcgc ctccagcacg gccagcagca
     1681 tcgccatccg cccgctgtcc cagcccacca cggcccggcg gggggtggcc ccgctctgca
     1741 accgcacgat cagggcctga acctcgatca gcacgggccg cgtgccctcg atcgccggga
     1801 acaccgcgct gccggccacc ggcgtctcgc ggcccgacag gaacagcatc gacgggttgc
     1861 cgacctcggc cagcccggcc ccttccatcg cgaaaacgcc gatctcgtcg accggaccga
     1921 accggttctt gatgcaacgc aggatccggt actggtggct gcgctcgccc tcaaagctca
     1981 tcaccacgtc gaccatgtgc tcgagcaccc gcgggccggc aatcgaaccg tccttggtga
     2041 catggcccac caggaacagc gccacgccgt tctccttggc atagcggatc agctcgaagg
     2101 ccgaggcgcg gacctggctg accgtgccgg gtgcgccctc gatctggtcg gaatgcatgg
     2161 tctggatcga atcgatcacc agcagcgccg ggggcggcat ggtggccagc gtggtcagga
     2221 tgtcgcgcac cgatgtcgcc gccgccagac tgatcggcgc cccggccagc cccagccggt
     2281 cggcccgcat acgcacctgg ccggccgctt cctcgccgct gatatagacc gcgctggccc
     2341 cgctttgggc gatgcgcgcg gccgcctgca gcagcagcgt gctcttgccg atgccgggat
     2401 cgccgcccat cagcacggcc gagccgggaa ccagcccgcc gcccagcgcg cggtcaaact
     2461 ccgcaaggcc ggtcgatttc cgcttcagca cctcgcccgg cgcatccagc gcttcgaacg
     2521 cgatggcccg cccgccgctg gacagatcat gtttcgccga aaagaccgtc gccggggctt
     2581 cctcggccag cgtgttccac tccccgcaat cggcgcattg gccctgccag cggtgggaga
     2641 cgctgccgca tgcctgacag atgtaacggc gtttcggttt ggccatggct caccgcatag
     2701 tgagaacaaa ccgggaccgc aaggcgaatt caggtgcgag ggacaatggg caggcgctgg
     2761 ttcagcggct tgcaatagcc ggccatctca aacgtattgt agagaggtat ggcgccaaag
     2821 gctctcccaa gcaggtggcg gttgcggatc gacatggcgc cggagttcaa gtcaatctgc
     2881 aacggagccg tcgttctctc gggcccgagc ggagcggact ggaaactggc gattccattg
     2941 gtaatggtca gcggttgcgg ctccttgaaa atcagctcgc cgccaagcgt cggcactacc
     3001 ccgaaaacct gaccggtctt gtttcggaaa tcgagattga cccgaatgtc ctgggtgtca
     3061 cggctggtca ccgttccatc gttgctgcgg atctggcttg tccccgtgca ttgaaggatc
     3121 accgagctta gacccgcgcc cgctggccgg gacttgcggc gcgtcgccgt ggccatcacg
     3181 accggctgga cggacggatt gatcagcatc tcaatattga cgtcggcaaa catagccttc
     3241 tcgtcctcca actaacctca aaccggctca agtccttgct ttcgcatctt gaacatttgc
     3301 agggaccgga atcaaggcta cttgttggcc atgcgccaaa aggaactccg tatcgccctc
     3361 gtctgctacg gcggagtgag cctggcggtc tacatgcacg gggtgaccaa ggagctgtgg
     3421 aagcttgccc gggcgagccg ggcctttcac ggcggcgcgg cgatttccgg cgggaccgaa
     3481 gcggtctatg tccggctgct ggagcggatc gaaaagcact tcaagctgcg cctgcgggtg
     3541 ctgcccgata tcgtcgccgg ggccagcgcg ggcgggatca acggggtgtt cctggcccaa
     3601 gcgatccatt ccgggcaaag cctggagccg ctgaccgagc tgtggctgga acgcgccgat
     3661 gtcgacgtat tgctcgatcc cgatgcccgg cccggatcgc ggatcgccaa gttctgggcc
     3721 atgccgatcg tctattggct gctcaagcgg cccggcaacg tggtctcgtc cagcgtcgcg
     3781 ccggaaaccc gcagcgaagt gcgggccaag ctctcgcgcc tgatccgctc gcgctggttc
     3841 gaaccgccgt tcggcgggct cggcttctcg cggctgctgt gcgaggcgct tggcacgatg
     3901 aaggcgggcg gcgccgacgc cccgctgctg ccggccggcc atccaattga cctgttcgtc
     3961 accgccaccg attttcgcgg ccatgcccag gtcctgcgcc tgcacagccc cgagcagatc
     4021 gaggagagcg agcaccgcct gtcgatcggc tttcgcagcc cgaccccggc cgagggcggc
     4081 ggcgctctgg ccgcgccgat cgagctggtc tttgccgcgc gggccacggc cagctttccc
     4141 ggcgcctttc cggccctgca gctggccgag atcgacagcc tggcccgcga gcacggcgat
     4201 ccctggcaca gccgcaccgc ctttatcgaa cggatcatgc ccgaacacag ccaccgcggc
     4261 gatgccgagc aggtggccct gatcgacggt tctgtgctgg tcaacgcgcc ctttgccgag
     4321 gccatggccg tgctgcgcga ccgtccggcc cagcgcgagg tggaccggcg cttcgtctat
     4381 atcgatcccc atcccgacga ggtgggcggc cagcgccatt ccgaaaccgg gccgccgggc
     4441 ttctttcagg tgatcttcgg ctcgctctcc tcgatcccgc gcgagcagcc ggtccgcgac
     4501 aacctggaag cgattgcccg ccagtcgcgc cagctggcgg gcctcaaggc catcgtcgat
     4561 tcgctccgcc ccgatgtgga ggaggcggtc gaacggctgt tcggacggac cctgtttctc
     4621 gaccggccga ccgtgaagcg gctctccgcc tggcgcgcca aggcccagca ggccgcggcg
     4681 gaacgcgccg ggttcacctt tcaggcctat gcccaggtca agctcgccgg gatcgtgcag
     4741 agcctggccg cgacgatcca gcaagccgcg cccgagctgg gcatggcggg ggacgaggcc
     4801 gtggccgacc ggctctccgc ctggcttgcc acccacggtc tcgatcacct cacggcatcg
     4861 agcggtgggg gcagtgccga ggcggtcgcc ttcttccgcg agcacgacct cggcttccgg
     4921 atccgccggc tgcggctgct cgcccggcgg ctgagcgaga actgggacga tatcgatgcc
     4981 caggcccccg aagcacggga agcggcccgc accgtcgtct acaaagcgct ctcgctctat
     5041 ttcgaacgcg acgcgagcgg atcgctgggc cgggatttcc cggacactgc ccgccgcgtc
     5101 ctgcaccatc ccggagaggt gctgagcgcc attgcccagc gccgccagct gccggcgacc
     5161 gacctcgtgg tcgatgccat gctcgtcgaa tgcctcgcca cgctcccgcg cgagctcaag
     5221 cggcggatcc tgctgaccta tctgggcttt ccgttctacg acaccgtcac cctgcccctg
     5281 ctgcgcggcg aaggacagac cgaattcgaa cccgccaagg tcgaccggat ttcccccgac
     5341 gattgcaact cgatccgcgg cggcggagcg agcgcggtgc tgcgcgggac cgaattctac
     5401 aacttcggcg ccttcttcag ccgcgcctac cgcgagaacg actatctctg gggccggctc
     5461 cacggggcgg aacggatgat cgacctgatc gcctcggccc tgcccacggg catggtgctc
     5521 gatccggccg agctggcccg gttcaagcgc gatgcctttc tggcggtgct ggacgaagag
     5581 gaagggcggc taaaggccga tccggggttg gtggctggag tacgggcgga agtgttaggc
     5641 gagcatgaat caatctgatc gcatgaattc agcttcaaac gggtgggaag ctgatccttg
     5701 ggatgcttcc gacgcggtcg ccgacctcca gcttgagggt tttcaaatca gagcagcgaa
     5761 ggcagtgagt tggtcggcaa tttctttggt cgaaggccca atttttggac atgatataga
     5821 gtggttgaat tcgctcgaag ttgaatatgt cgcaacccac gatctcgagg actggttgct
     5881 aatgcgcctc gtctatcatg gatttcccga tcctccagaa tggcggctgg cgacgagagc
     5941 cgctggaaaa ttcagccatc catggaattc gtggggatac ttcgcgcagt tgcccgacgc
     6001 gtggaagatt cccggtattt gaacacttgc tcgtaagatt tcatccgtcg atgcctgacg
     6061 gaaggacaag cacctacccc ccgaatgctt ccttgatccg gtcaaagaac cccttcgatt
     6121 gcggcgtttc ctcacccgtc tcggtggcct gcagttcgcg cagcagttcc ttctggcggc
     6181 tggtcagctt ggtcggggtt tcgaccataa tctcgatcac cagatccccg cggccccgtc
     6241 cctgcaggac cggcatgccc gaaccgcgct tgcgcag
//
LOCUS       DASUDV010000233         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1332541,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000233 DASUDV010000000
VERSION     DASUDV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332541"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VFV30_05050"
     CDS             complement(<1..1167)
                     /locus_tag="VFV30_05050"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX4847487.1"
                     /translation="MASDWNPDSWTAKEARHLPRYPDAAALDKVTGTLGGFPPLVFAG
                     EARALKADLAEVAAGKGFLLQGGDCAESFAEFHPNNIRDTFRVLLQMAVVLTFASKQP
                     VVKVGRMAGQFAKPRSSPVEVIDGVELPSYLGDNINGIEFSAESRIPDPQRLMQAYSQ
                     SAATLNLLRAFSTGGYANLRQVHQWTLDFMGRSPWAAKFAEVSTRIGEALDFMEACGI
                     DPETVPQLKGTTFYTSHEALHLPYEQAMTRRDSLTGDWYDTSAHMLWIGDRTRFEGSA
                     HVEFLRGVGNPIGFKCGPSLAPDELLRQLDVLNPGREAGRITLISRFGDDKVEAGLAP
                     LVRAVKREGHPVVWSCDPMHGNVIKADSGYKTRPFDRILGEVRGFFAVHRAEGTH"
     gene            complement(1296..4292)
                     /locus_tag="VFV30_05055"
     CDS             complement(1296..4292)
                     /locus_tag="VFV30_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX4847488.1"
                     /translation="MHKPSRAARFFAFVLPALAMIATPALEAATAKGKRPAAARAAPA
                     KAPAKAPAPKCDQSVPWLYRCSDIPIDKEWQFGELPNGVRYVVRRNGVPPGQVSIRVR
                     MDVGSLYERDNELGFSHLLEHMVFRQSKYLGDGEAIPTWQKMGATFGSDTNAETSPIA
                     TTYKLDLPNATEASFDESMKLLSGMMIAPVLSEKNVRTDVPIVLAEMRERGGTAARVF
                     EQTQQTLFAGQKLAVRPVIGTEATLNGANAASVQAFHQRWYRPENAVVIVVGDLPPAA
                     MEAAIRKWFADWQGVGPRTPEPPFGDPLPPKGAAGVAPVGETRVIVEPDLPRSITYAV
                     MRPWRPVRDTIVYNQGIMVESLAQAIINRRLEAKARAGGSFLVAQVSKDQVVRSADAT
                     YVMITPLGPDWKSALGDTRAVIADAMAKPPTQEEIDREVKEFGVAFESSVEQRRLLPG
                     ARLADNMVTALDIRETIASPEVVQDIFNRSKPLFTPANVLAATRAMFRGSVTRSVYVT
                     PQAGEATPAALRAALAVPVAANSGSRLAAANIKFSDQPAIGAVATPTSERATGLLEIK
                     QIDYANGVRVQLWPTEDEPGRVTLKVRWGGGWRAFTAQTAPYATLGDMALIDSGVGQW
                     GREEMDRAMTGRKMGFDFEIGDTSFLFKADTRSADLDDQLYLFAAKFAAPRWDANPVL
                     RAKAAAKINYESYASSPQGVLERDLKFYERGRDPVFRTPTPAELEQTTPEGFRQVWEP
                     ILKQGPMEIQLFGDFDEAKAIAALNRTFGALPARGELPAAQTSLAARTLPPGGDPILL
                     FHRGDGNQAAGIIGWPTAGGQAALRESRQLEILSQVFQNRLMNKLRESLGEAYSPSVI
                     NAWPQELNGGGNILAISNLQPKTVPVFFTVADAIAQDLVDKPITEEELALVTEPLKQQ
                     LTRASSSSAFFMYLIEGATTDPVRYRGVRNVMNDYTMTTPEAMQALARKYLGTGKSWR
                     IAIIPQGQSLAPMPVPASLAAPAPGR"
BASE COUNT          657 a         1462 c         1463 g          732 t
ORIGIN      
        1 gtgggtgcct tcggcgcggt gcaccgcgaa gaagccgcgc acttcgccca ggatccggtc
       61 gaacggccgg gtcttgtagc cgctgtcggc cttgatcacg ttgccgtgca tcggatcgca
      121 cgaccagacc accggatggc cttcgcgctt caccgcgcgg accagcgggg ccaggcccgc
      181 ttcgaccttg tcatcgccaa agcggctgat cagcgtgatc cgcccggctt cgcggcccgg
      241 gttgagcacg tcgagctggc gcagcagctc gtccggcgca aggctgggac cgcacttgaa
      301 gccgatcgga ttgccgaccc cgcgcaggaa ctcgacatgg gccgagcctt cgaaccgggt
      361 ccggtcgccg atccacagca tgtgggcgct ggtgtcatac cagtccccgg tcagcgaatc
      421 ccgccgggtc atggcctgct catagggcag gtgcagcgcc tcgtggctgg tgtagaaggt
      481 cgtgcccttc agctgcggca cggtttcggg atcgatcccg caggcttcca tgaagtccag
      541 cgcttcgccg atccgggtcg aaacctcggc gaacttggcc gcccacgggc tgcggcccat
      601 gaaatcgagc gtccactggt ggacctggcg caggttggca tagccgccgg tcgagaaggc
      661 gcgcagcagg ttgagcgtcg ccgcgctttg cgaataggcc tgcatcagcc gctgcggatc
      721 ggggatccgg ctttcggccg aaaattcgat accgttgatg ttgtcaccca ggtagctcgg
      781 cagctcgacc ccgtcgatca cctcgaccgg cgaagagcgc ggcttggcga actggccggc
      841 catccggccc accttcacca ccggctgctt gctggcaaag gtcagcacga ccgccatctg
      901 cagcagcacg cggaaggtgt cgcggatgtt gttggggtga aactcggcaa agctttcggc
      961 gcagtccccg ccctggagca ggaaaccctt gccggccgcc acttcggcca ggtccgcctt
     1021 cagcgcgcgg gcttccccgg cgaagaccag cgggggaaat ccgcccagcg tcccggtcac
     1081 cttgtccagc gcggcagcat cgggatagcg cggcagatgc cgtgcttcct tggccgtcca
     1141 gctatcaggg ttccagtcac tcgccacggg tcacgctccg gttagaaaca aagggcggcc
     1201 catagccgcc ctttgctgca aatgcaaaac ctgcaaaccc gatgagtgca atcgggcaat
     1261 aatgttgcgc gcagaccggg cggtctgccc cgccctcagc gcccgggcgc cggtgcggcc
     1321 aggctggccg gcaccggcat cggggccagg ctttgcccct gcgggatgat cgcgatccgc
     1381 cacgacttgc ccgttcccag gtacttgcgg gccagggcct gcatcgcctc gggcgtggtc
     1441 atcgtgtaat cgttcatcac gttgcgcacc ccgcggtagc ggaccggatc ggtggtcgcc
     1501 ccttcgatca ggtacatgaa gaacgcgctg gacgaggacg cccgggtcag ctgctgcttc
     1561 agcggctcgg tcaccagggc cagttcctct tcggtgatcg gcttgtcgac cagatcctgg
     1621 gcgatggcgt cggccacggt gaagaacact ggcacggtct tgggctgcag gttggaaatg
     1681 gcgaggatgt tgccgccccc gttcagttcc tgcggccagg cgttgatcac cgacggcgaa
     1741 taggcttcgc ccaggctttc gcgcagcttg ttcatcagcc ggttctggaa cacctgcgag
     1801 aggatctcca gctggcggct ttcgcgcagc gcggcctgcc cgccggcggt cggccagccg
     1861 atgatcccgg cggcctggtt gccgtcaccg cggtggaaca gcaggatcgg atcgccgccc
     1921 ggaggcaggg tgcgggccgc caggctggtc tgcgccgcgg gcagttcgcc ccgtgccggc
     1981 agtgcgccga aggtgcggtt gagcgcggca atcgccttgg cttcgtcaaa gtcgccgaac
     2041 agctggatct ccatcggacc ctgcttcagg atcggttccc acacctggcg gaagccttcc
     2101 ggcgtggtct gctccagctc ggccggggtc ggggtgcgga acaccggatc cctgccgcgt
     2161 tcgtagaact tgaggtcgcg ttccagcacg ccctgcgggc tggaggcata gctttcgtaa
     2221 ttgatcttgg ccgcggcctt ggcccgcagc accgggttgg catcccagcg cggcgcggcg
     2281 aacttggcgg cgaacagata gagttggtcg tccagatcgg cagagcgggt atcggccttg
     2341 aacaggaagc tggtgtcccc gatctcgaaa tcgaagccca tcttccgccc ggtcatcgcc
     2401 cggtccatct cttcccggcc ccactggccg acgccggaat cgatcagcgc catgtcgccc
     2461 agcgtggcat agggcgcggt ctgggcggta aaggcccgcc agccgccgcc ccagcgcact
     2521 ttcagcgtca cccgcccggg ctcgtcctcg gtcggccaga gttggacccg gaccccgttg
     2581 gcatagtcga tctgcttgat ttccagcagc ccggtcgccc gttcagaggt cggcgtggcc
     2641 accgcgccga tcgcgggctg gtcggagaac ttgatgttgg ccgcggccag gcgcgatccg
     2701 gaattggccg ccaccggcac ggccagcgcg gcgcgcagcg cggccggcgt ggcttccccg
     2761 gcctgcgggg tgacatagac cgagcgggtc acgctgccgc ggaacatcgc gcgggtcgcc
     2821 gccagcacat tggccggggt gaacagcggc ttggaccggt tgaagatgtc ctgcaccact
     2881 tcgggcgagg caatcgtctc gcgaatgtcg agcgcggtga ccatgttgtc ggccagccgg
     2941 gccccgggca gcaggcggcg ctgctcgacg ctgctttcaa aggcgacgcc gaattccttc
     3001 acctcgcggt cgatttcctc ctgcgtcggc ggcttggcca tggcatcggc aatcaccgcg
     3061 cgggtatcgc ccagcgccga tttccagtcc ggccccagcg gggtgatcat cacataggtc
     3121 gcatcggccg agcggacgac ctggtccttg ctgacctggg ccaccaggaa cgaacccccg
     3181 gcccgggcct tggcctccag ccggcggttg atgatcgcct gggccagcga ttccaccatg
     3241 atcccctggt tgtagacgat cgtatcgcgg accggacgcc acgggcgcat caccgcatag
     3301 gtgatcgagc gcggcagatc gggttcgacg atgacgcggg tttcgcccac cggggccacg
     3361 ccggccgcgc ccttgggcgg cagcggatcg ccgaacggcg gctccggcgt gcgcgggcca
     3421 acgccctgcc aatcggcaaa ccacttgcgg atcgcggctt ccatcgccgc cggcggcaga
     3481 tcgccgacca cgatcaccac cgcgttttcc gggcggtacc agcgctggtg aaacgcctgg
     3541 accgaggccg cgttggcgcc gttcagcgtc gcttcagtcc cgatcacggg gcgcacggcc
     3601 agcttctgac cggcaaacag ggtctgctgg gtctgctcga acacgcgggc cgcggtgccg
     3661 ccgcgctcgc gcatttcggc cagaacgatc ggcacgtcgg tgcgcacgtt cttttcgctc
     3721 agcaccgggg caatcatcat gcccgacagc agcttcatcg attcatcgaa gctcgcctcg
     3781 gtcgcgttgg gcaggtcgag cttgtaggtc gtcgcgatgg gcgaggtttc ggcattggtg
     3841 tcgctgccaa aggtcgcgcc catcttctgc caggtcggga tcgcctcgcc atcgcccagg
     3901 tacttcgact ggcggaacac catgtgctca agcaggtgcg agaagcccag ctcgttgtcc
     3961 cgttcgtaca gcgagccgac gtccatccgc acccggatcg aaacctggcc cggcggcacg
     4021 ccgttgcggc gcacgacata gcgcaccccg ttgggcagct cgccgaattg ccattccttg
     4081 tcgatcggga tgtcgctgca gcggtagagc cacggcacgc tctgatcgca cttcggcgcc
     4141 ggggccttgg cgggggcctt ggccggggcg gcgcgggccg cggcgggccg cttgcccttg
     4201 gccgtcgccg cctccagcgc gggcgtggca atcatggcca gggcgggaag aacgaaggcg
     4261 aagaagcgcg cggcgcggga aggcttgtgc atagggctgc gactataggc agcg
//
LOCUS       DASUDV010000234         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_758457,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000234 DASUDV010000000
VERSION     DASUDV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758457"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFV30_05060"
     CDS             complement(<1..502)
                     /locus_tag="VFV30_05060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX4847489.1"
                     /translation="MSDFLLELRSEEIPARMQAGARADLEKLFRKEMDAAGVAVGDIT
                     VWSTPRRLALIARGLPAATAAVSEEVKGPRVGAPPQALEGFLRKTGLTEDQLVERDGV
                     LFAVTEKPGRATAEVLAEAIPAIVRAFPWPKSQRWGAASISTESLRWVRPLSAIVALF
                     DGQVVDC"
     gene            complement(499..1401)
                     /locus_tag="VFV30_05065"
     CDS             complement(499..1401)
                     /locus_tag="VFV30_05065"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX4847490.1"
                     /translation="MSDNTRPLSFQEMILRLHAFWSAQGCLILQPYDMRMGAGTFHPA
                     TTLRALGPEPWKAAYVQPSRRPTDGRYGENPNRLQHYYQYQVILKPNPENLQELYLQS
                     LAEIGVDPLAHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVTQFTYFQQVGGFDCKPV
                     AGELTYGLERLAMYIQGVDNVYDLAFNAEGVSYGQVFLKNEQEHSKYNFEVADTAQLF
                     EGFRNAERECQRCIEAQVPLAAYDQAIEASHLFNLLQARGVISVQERASYIGRVRDLA
                     KGSCQAWIEKNKAEWEAKYPGWNV"
     gene            1521..>2295
                     /locus_tag="VFV30_05070"
     CDS             1521..>2295
                     /locus_tag="VFV30_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX4847491.1"
                     /translation="MIARFLSPLALLLSAAVPAQQSLPTEQPVVAEPAAVEVHPALWR
                     VADEDTTVYLFGTIHLLPQGLNWFQGPLAEAFAAADELVTEIPEIDPQASQAVLLKRG
                     ILPAGQSLRDQMTPKQRLLFEKTLASFDLAPAAFDRFKPWYAAVVLSTLPLQRKGYSL
                     ANGVETDLANRNAALQRSRIGLETLDYQLGLFDAFPAKVQRRYLFEVIAALPQIDKDV
                     GEMVEAWRSGDAAKLATLMNADQDDPAMVAALLTNRNKAW"
BASE COUNT          382 a          770 c          722 g          421 t
ORIGIN      
        1 cgcaatcgac gacctgccca tcaaacagcg ccacaatggc cgatagcggc cgcacccagc
       61 gcaggctttc ggtgctgatc gaggccgccc cccagcgctg gctcttgggc caggggaaag
      121 cgcggacgat cgcggggatc gcttcggcaa gcacttcggc ggtggcgcgg ccgggctttt
      181 cggtgaccgc gaacagcacg ccatcgcgtt cgaccaactg gtcttcggta agaccggtct
      241 tgcgcaggaa accttccagc gcctgcggcg gcgcgccgac acgcggcccc ttcacttctt
      301 ccgaaaccgc cgccgtcgcc gccggcaggc cgcgcgcgat cagcgcaagg cggcgcgggg
      361 ttgaccagac ggtgatatcg cccacggcaa cgccggcggc gtccatttcc ttgcggaaca
      421 gcttttccag gtcagcgcgc gcgccggcct gcatccgcgc agggatttcc tcggagcgga
      481 gctcaagcag gaagtcgctc atacattcca ccccgggtac ttcgcttccc attcggcctt
      541 gttcttctcg atccaggcct ggcacgatcc cttggcgaga tcgcggaccc ggccgatgta
      601 gctggcgcgt tcctggacag agatcacgcc gcgcgcctgc agcaggttga acaggtggct
      661 cgcctcgatc gcttggtcat aggccgccag cggcacctgg gcttcgatgc agcgctggca
      721 ttcgcgctcg gcattcctga agccttcgaa cagttgggcc gtatcggcca cttcgaagtt
      781 gtacttcgaa tgctcctgct cgttcttcag gaagacctgg ccatagctga cgccttcggc
      841 gttgaaggcc aggtcataaa cgttgtcgac gccctggatg tacatcgcca ggcgctcaag
      901 cccgtaggtc agctcgcccg ccaccggctt gcaatcgaac ccgccgacct gctggaaata
      961 ggtgaactgg gttacttcca tcccgtcgca ccagacttcc cagcccaggc cccaggcgcc
     1021 cagcgtcggg ctttcccagt catcctcgac aaagcggatg tcgtgcgcca gcggatcgac
     1081 gccgatttcg gccagtgact gcagatacag ttcctgcagg ttttccgggt tcggcttcag
     1141 gatcacctgg tactggtaat agtgctgcag ccggttcggg ttctcgccat agcgaccatc
     1201 ggtcgggcgg cggctcggct ggacataggc ggccttccac ggctccggcc ccagcgcgcg
     1261 cagcgtggtg gcggggtgaa aggtgccagc ccccatgcgc atgtcatagg gctgcaggat
     1321 caggcagccc tgcgcgctcc agaacgcgtg gagccgcagg atcatttcct ggaacgaaag
     1381 cgggcgagtg ttgtccgaca tggccgaggc ctttggcgga tgggctcttt ccggtcaacc
     1441 ggcgaggcaa tcctctgggc aaggttccgc taagcgtgat cgttcatcga cgcgcccact
     1501 tcttgcgcta tagcggcgcc atgatcgcgc gtttcctctc gcccctcgcc ctgctgctct
     1561 cggccgctgt cccggcccag caatcgctgc cgaccgagca gccggtggtg gcggagcccg
     1621 ccgcagtaga ggttcacccg gcactttggc gcgtagccga tgaggatacg acggtttacc
     1681 tgttcggcac gatacacctg ctgccgcagg gtctgaactg gtttcaggga ccgcttgccg
     1741 aggcattcgc cgcggctgac gagctggtga ccgaaattcc cgagattgat ccgcaagcca
     1801 gccaggctgt gctgctgaag cggggcatcc ttccggccgg gcaaagcctg cgcgatcaga
     1861 tgacgcccaa gcaacgcctg ctgtttgaaa agacgctggc cagcttcgac cttgcgccag
     1921 cggcctttga ccggttcaag ccgtggtatg ccgccgtcgt gctttcgacc ctgccgttgc
     1981 agcgcaaggg ctattcgctg gccaacgggg ttgagaccga ccttgccaac cgcaacgcgg
     2041 cgcttcagcg gtcacggatc gggctggaaa cgctcgacta ccagcttggc ctgttcgatg
     2101 ccttcccggc caaggtccag cgccgctacc tgttcgaagt gatcgccgcc ctgccccaga
     2161 tcgacaagga cgtgggcgag atggtcgagg cctggcgctc cggcgatgcc gccaagctcg
     2221 ccacgctgat gaacgccgat caggacgatc cggccatggt cgcggcgctg ctgaccaatc
     2281 gcaacaaggc ctggg
//
LOCUS       DASUDV010000235         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1863104,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000235 DASUDV010000000
VERSION     DASUDV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863104"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFV30_05075"
     CDS             complement(<1..1162)
                     /locus_tag="VFV30_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX4847492.1"
                     /translation="MNQGTATSVRPQRSGGRSARREARGGGPQAAGKPYIIRNIPTYD
                     IMGEESLLRIEQTADRILAEVGIEFRDDPETLELWKRAGARVDGVLVRFEPGMLREIL
                     KTAPQEFTQHARNPANNVEIGGRNVVFSPSYGSPFVMDLDKGRRYGTIEDFRNFIKLA
                     QSSPWLHHSGGTICEPVDVAVNKRHLDMVYSHVRYSDRAFLGSVTAESRAQDSIEMAR
                     IVFGRDFVDRNCVIMGNVNVNSPLVWDGVMTSVIRAYADANQGSVIVPFILAGAMGPV
                     TNAGAIAQSLAETMAGCALTQVVRPGAPVIFGNFLSTMSLRSGSPTFGTPEPAIGSMV
                     VGQLARRLNLPLRCSGSFTTSKLPDAQAMNESTMSMLAAVHCGANFLLHSAGF"
     gene            1258..2145
                     /locus_tag="VFV30_05080"
     CDS             1258..2145
                     /locus_tag="VFV30_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4847493.1"
                     /translation="MLRRSIPSLNSLTVFEAAGRLASFSAAARELGVSQAAVSYAIAR
                     LEEQLGATLFHRQYRRVRLTEAGQKFHADVSIGLLHIQRSATELRAGQSGHVTLACST
                     AFAAYWMVPRMQALREALPGTDLRIQTADRDLDLLDEGIPLGIRGGLAEDWPDLESRQ
                     LSGETIYPVCSPLYLARFARPESPEALLEHRLIHLEEPFRTAATWAEWFAAAGISAGR
                     VPGGLQINDYVLVIQAVIEGQGIALGWHHLVQDLVARGVLVRLTDVALETGRGFHIIW
                     RRGELLSPAAERVRDWLLA"
     gene            complement(2157..>2570)
                     /locus_tag="VFV30_05085"
     CDS             complement(2157..>2570)
                     /locus_tag="VFV30_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX4847494.1"
                     /translation="GDASIAAVVSGQAADLSAASLETQLFVLLKSLPLAQVTSFAAVV
                     LILVFFVTGWDSGTLVIDTMTSGGRTDTPLRQKVFWLLAVGGIGVMLLLTGGLNSLQA
                     GAIATGLPLALVLLAMCVGTLRGLLALHRRHSHED"
BASE COUNT          450 a          812 c          841 g          467 t
ORIGIN      
        1 ggaacccggc cgaatggagc aggaagttcg cgccgcaatg caccgccgcc agcatcgaca
       61 tggtgctttc gttcatcgcc tgggcatcgg gcagcttcga ggtggtgaag ctgcctgaac
      121 agcgcagtgg caggttgagc cgccgggcca gctggcccac aaccatcgat ccgattgccg
      181 gttcgggcgt gccgaaggtg gggctgcccg agcgcagcga catcgtcgag aggaaattgc
      241 cgaagattac cggcgcgccg gggcggacca cctgggtcag cgcgcagccg gccatggttt
      301 cggccagcga ctgcgcgatt gctccggcgt tggtcaccgg ccccatcgcg ccagccagaa
      361 tgaaaggcac gatcaccgaa ccctggttgg catcggcata ggcgcggata acgctggtca
      421 tcaccccgtc ccagaccagc ggggaattga cgttgacgtt gcccatgatc acgcagttcc
      481 ggtcgacaaa gtcgcgcccg aacacgatcc gcgccatctc gatcgaatcc tgtgcgcggc
      541 tttcggcggt gaccgatccc aggaacgcgc ggtccgaata gcggacatgc gaatagacca
      601 tgtcgaggtg gcgcttgttg acggcaacgt ccacgggctc gcagatcgtc ccgcccgaat
      661 ggtgcagcca gggagaggac tgggccagct tgatgaaatt gcggaagtcc tcgatcgttc
      721 cgtagcggcg gcccttgtcc agatccatca cgaaggggct gccatagctg ggcgaaaaga
      781 ccacgttgcg cccgccgatt tcaacgttgt tggcggggtt gcgggcgtgc tgggtgaatt
      841 cctgcggtgc ggtcttcagg atctcccgca gcatgccggg ttcgaacctg accagcacgc
      901 cgtcgaccct ggccccggcg cgcttccaca gttcgagcgt ttcgggatcg tcgcgaaatt
      961 cgattcccac ctcggccagg atccggtcgg cggtctgctc gatccgcagc agcgattcct
     1021 cgcccatgat atcgtaggtg gggatgttgc ggatgatgta gggcttgcct gccgcctgcg
     1081 gtccgccccc gcgcgcctcg cgccgggcgc tgcggccacc gctacgttgt ggccggacgc
     1141 ttgttgcggt cccctggttc atcacagcct ccataaattc gagtcgaaac tagggtcggg
     1201 gagggcctgt gtaacgctgg aaaagtttca tcgaactgat aaggtgaact tatggaaatg
     1261 ctgcgtcgtt cgattccttc gctcaattcg ctcacggttt tcgaagcggc ggggcggctt
     1321 gccagcttca gcgccgccgc gcgcgaactc ggagtcagcc aggcggcggt gtcctatgcg
     1381 atcgcgcgac tggaggaaca gctgggcgca acgctgttcc atcgccagta tcgccgggtt
     1441 cgcctgaccg aggccgggca gaaattccac gctgacgtct cgatcggact gctgcatatc
     1501 cagcgttccg caaccgagct gagagctggc cagagcggtc atgtgaccct ggcctgttcg
     1561 actgcgtttg cagcctactg gatggttccg cgcatgcagg cgctgcgcga agcactgccg
     1621 ggaaccgacc tgcggattca gaccgccgac cgcgacctcg acctgctcga tgaaggtata
     1681 ccgctcggca ttcgcggcgg gctggccgag gattggcccg acctggaaag ccggcaactg
     1741 tccggagaaa cgatataccc ggtttgcagt ccgctctatc tggcccgctt cgcccgtccc
     1801 gaaagcccgg aagcattgct tgagcatcgg ctgatccacc ttgaagagcc tttccgcacg
     1861 gcggcgacct gggccgaatg gttcgcagcc gcggggatct ccgccgggcg ggtgccaggc
     1921 gggctgcaga tcaatgatta tgtgctggtg atccaggcgg ttatcgaggg tcaggggatc
     1981 gcgctgggtt ggcaccatct ggttcaggat ctggtggcca ggggcgtgct tgtgcgcctg
     2041 accgatgttg cgctcgaaac cggccgcggc tttcacatca tctggcggcg cggcgaactg
     2101 ctttctccgg cggccgaacg ggttcgcgac tggcttttgg catagcgggc tgcggttcag
     2161 tcctcgtgac tgtggcgccg gtgcagcgcc agcagccctc gcagggtgcc tacgcacatt
     2221 gccagcagca ccagcgccag tggcagcccg gtggctatcg ccccggcctg gagggagttc
     2281 agcccgccgg tcagcagcag catcaccccg attccgccaa ccgcaagcag ccagaacacc
     2341 ttttgcctca gcggcgtgtc ggtccgcccg cccgaggtca tggtatcgat caccagggtg
     2401 cccgaatccc agcctgtgac gaagaacacc aggatcagta ccaccgcggc gaatgaagtg
     2461 acctgcgcca gcggcagcga tttgagaagt acgaacagct gcgtctccag gctggctgcg
     2521 gaaagatcgg cagcctggcc tgaaaccacc gcagcgatcg acgcatcgcc
//
LOCUS       DASUDV010000236         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_206370,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000236 DASUDV010000000
VERSION     DASUDV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206370"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFV30_05090"
     CDS             <1..597
                     /locus_tag="VFV30_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847495.1"
                     /translation="EAVLGGKTALGEALRYLADVPRDAKGKLPKLSKRSVILFARPVA
                     GRPGELQLVAPDAQLLWDLTLEARVKALLGEFYAPSAPRAISGLREAIHVPGALAGEG
                     ETQFFLTTTGGEPAAITVTRAPGSEPRWTVSFSELVETGAPPPARETLAWYRLACFLP
                     RQLVPGANVSSSPEDRQAAARDYRFVLEQLGPCPRLRR"
     gene            637..1947
                     /locus_tag="VFV30_05095"
     CDS             637..1947
                     /locus_tag="VFV30_05095"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX4847496.1"
                     /translation="MAEPLRVALAGLGTVGAGVIRLVEANADLITRRAGRPIQITAVS
                     ARDRSRDRGVDISRYAWEDDMTVMAGRGDVDLVVELVGGSDGPALALARRTLGAGKGL
                     VTANKAMIAHHGMELAALAEQHGAPLRFEASVAGGIPVIKGLREGVAANRVERIYGIL
                     NGTCNYILSTMEDTGRDFAEVLAEAQAKGYAEADPTFDIEGTDAAHKLSILSAIAFGS
                     TIDFASVETSGISRILAADIAQADALGYVIRLIGMAEAGDGGLFQRVQAHLVPFDNLL
                     AHVDGPTNAVVVEGNFSGRLLFQGAGAGDGPTASAVVADIIDIARGMDTGFEADRAFS
                     IPVAGLEAMAVADSGTRQSRAYIRFMVADRPGVLAELTAAMRDAGVSIESLIQKGRAG
                     DAGAVMVAMVTHEGPESAVAEALRLLDGSASLTEPPLVMHLLGE"
     gene            complement(1951..2367)
                     /locus_tag="VFV30_05100"
     CDS             complement(1951..2367)
                     /locus_tag="VFV30_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847497.1"
                     /translation="MAALAAMPLLAQSADPEPDPQIAQRLAAAKSRLSVETVQDRCRS
                     ARKAGEIVVCVDRGEDLRVPTTAESDPDSLAARRALNNGVPRAPAFSRSCKGQPGCIT
                     GGWAPPPIYVIDPAALPEAPEGSDADKIAKGELSDR"
BASE COUNT          371 a          794 c          856 g          410 t
ORIGIN      
        1 gaagccgtgc tcggcggcaa gaccgccttg ggcgaagcgc tgcgctatct ggctgacgtg
       61 ccgcgcgatg cgaaaggcaa gttgcccaag ctgtcgaagc gtagcgtgat cctgtttgcg
      121 cggcccgtgg cgggccgtcc cggcgaattg cagctggtag cgcccgatgc ccagctgctg
      181 tgggatctga ccctggaagc gcgcgtgaag gcgctgctgg gcgaattcta tgccccctcg
      241 gccccgcgtg cgatcagcgg cctgcgcgaa gccatccacg tgccgggcgc gctggcgggt
      301 gagggtgaaa cccagttctt cctgaccacc accggcggtg agccggcggc gataacggtg
      361 acccgtgcgc cgggcagcga accgcgctgg acggtatcct tttcagaact ggttgagacc
      421 ggggcgccgc ccccggcgcg tgaaacgctg gcctggtatc gcctggcctg cttcctgccg
      481 cgccagcttg tgcctggtgc caacgtctcg tcctcacccg aggatcggca ggcggccgcg
      541 cgcgactatc gcttcgtgct ggaacaattg gggccttgcc cgcggttgcg tcgctagcac
      601 agctcgctta gggcgcgcgg gcgaaagggg gcgatcatgg ccgaaccgtt gagagtggca
      661 ctggcaggac ttggcaccgt gggggccggg gtaatccgcc tggtcgaggc caatgccgac
      721 ctgatcaccc gccgggccgg ccgcccgatc cagatcaccg cagtcagcgc gcgcgatcgc
      781 agccgcgacc gcggcgttga tatttcccgc tacgcctggg aagacgacat gaccgtgatg
      841 gcgggtcgcg gcgacgtcga cctcgtggtc gaactggtcg gtggctcgga tggtccggcg
      901 cttgcgctgg cgcgcaggac gcttggcgcc ggcaaggggt tggtcaccgc caacaaggcg
      961 atgatcgcgc atcacgggat ggagctggcc gcgctcgccg aacagcatgg tgcgccgctg
     1021 cggttcgaag cttcggtcgc agggggcatc ccggtgatca agggcctgcg cgaaggggtg
     1081 gccgcaaacc gggtcgagcg gatctacggg atcctcaacg gcacctgcaa ctatatcctc
     1141 tcgaccatgg aggacaccgg ccgcgacttt gccgaggtcc tcgccgaggc gcaggccaag
     1201 ggctatgccg aggccgaccc gactttcgat atcgaaggca ccgatgcggc ccacaagctt
     1261 tcgatccttt cggcaatcgc tttcggctca acgatcgatt tcgcctcggt cgagaccagc
     1321 ggcatttcgc gaatccttgc cgccgatatc gcccaggccg atgcgctggg ctacgtcatc
     1381 cgcctgatcg gcatggccga agctggtgat ggtggcctgt tccagcgggt ccaggcgcac
     1441 ctcgtcccgt tcgacaatct cctcgcccat gtcgatggtc caaccaatgc ggtggtggtc
     1501 gagggcaact tttccggccg gctgctgttc cagggggccg gtgccggtga cgggccgacc
     1561 gccagcgccg tggtcgcaga tatcatcgat atcgcgcgcg ggatggacac cgggttcgag
     1621 gccgaccgtg ccttctcgat tcctgtcgcc gggctcgaag ccatggcggt tgccgacagc
     1681 ggaacgcgcc agagccgggc ctatatccgc ttcatggtgg ccgaccggcc tggcgtgctg
     1741 gctgagctca ccgccgccat gcgcgatgcc ggggtctcga tcgaaagcct gatccagaag
     1801 ggccgggcgg gcgatgctgg cgccgtgatg gttgcgatgg tgacgcacga gggcccggaa
     1861 agcgccgtgg ctgaggcgct gcgcctgctc gatggatcgg ccagcctgac cgagccgcca
     1921 ttggtgatgc atctgctggg cgaatagccc tcagcggtcg gacagttcgc ccttggcgat
     1981 cttgtcggca tccgaacctt ccggggcttc cggcaaggcg gcaggatcga tcacgtagat
     2041 cggcggcggg gcccagccgc cggtgatgca gcctggctgg cctttgcagg acctagaaaa
     2101 ggccggcgcg cgcggcacgc cattgttgag cgcgcggcgg gcggcgagcg aatctggatc
     2161 gctctcagcc gtggtcggca cgcgcaggtc ctcgccgcga tcgacgcaga caacgatttc
     2221 gcctgccttg cgggccgagc ggcaacgatc ctggaccgtt tcgacgctga gccgcgactt
     2281 ggcggcagcc aggcgctggg cgatctgcgg atcgggttcc gggtcggcgc tctgggccag
     2341 cagcggcatg gccgccaagg cggccaacag tccaattgat cctcccggcc agcccatcac
     2401 cgaacttgac cgcgctggcg cttaatcccc g
//
LOCUS       DASUDV010000237         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_140069,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000237 DASUDV010000000
VERSION     DASUDV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140069"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFV30_05105"
     CDS             complement(<1..378)
                     /locus_tag="VFV30_05105"
                     /inference="COORDINATES: protein motif:HMM:NF022436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEX4847498.1"
                     /translation="MPDPLFDSRTVRLELPLLFAGQSQKENYVNEIAARIDALLHGAI
                     EAEANSPPTSPADGACWLVGSSPTGDWTGKAGKIAARQAGNWLFFTPRDGMVLLNRST
                     GQQVRFVAGWQAPARPTAPTGGTT"
     gene            complement(398..2587)
                     /locus_tag="VFV30_05110"
     CDS             complement(398..2587)
                     /locus_tag="VFV30_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX4847499.1"
                     /translation="MATLVLTVVGTMLGGPLGGAIGSMVGRQVDSMIFGRGGSREGPR
                     LKELSVTTSSYGMPIPRHFGTIRVPGQIIWATDLVEHKEKQGGGKGRPSVVTYTYSAS
                     FAVALSSRPVGAIGRIWADGKLLRGAAGDLKVAGTMRLHSGRGDQGADPLLVAAEGAS
                     LCPAYRNLAYVVFEDLQLAEYGNRIPTLSFEVIADPGPLGLAPVFVGAVDDIDADVPL
                     PGLTGLTCDGSVGELLGVLDPLFPVDCDACDERLAIRPDRNQAAVIALPEAATSTRRE
                     DFGGNAGFARKRGAQPEAPIAVLRYYDVERDYQPGAQRAAGRPHPGEPRTIELPAALN
                     AAAARQLVEGAAKRINWARQTISWRVTQIDPAVRPGAVVSLPNHAGLWRVREWEWHEQ
                     GIDLSLARLSPTLELPAAPADPGRTNPPLDGALATTSLAACELPWDGNPATPVPLILA
                     ACSSPGSSWGGASLFVDQGDGSLLPVGSSGRDRAVLGTAITGLGSATPLLFDRHNSVT
                     IQLVAPDLVMGNATMRQLAMGANRALLGSEIIQFAEAVQLSASQWHLSGLWRGRGGTE
                     QAVASHASGESFVLLDGSGTQLDPALVGQVPDTLVAALGLGDTAPVTAPIALRGIGAR
                     PLSPVHLRLETLPSGTTRLSWVRRARGAWFWQDGAEVALGEQTEAYGVTFGTATTVLA
                     TWQTGQPWLELDAAEFAALASAAPGGSFTVRQIGDRAQSLPASLAIA"
     gene            complement(2599..>2791)
                     /locus_tag="VFV30_05115"
     CDS             complement(2599..>2791)
                     /locus_tag="VFV30_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847500.1"
                     /translation="VAERPFVPGDVVLLAPGPAQFHLAIAAADGAWIHAHAGLRRVVR
                     EPGLPAGTQTHHWRLLPNP"
BASE COUNT          458 a          945 c          961 g          427 t
ORIGIN      
        1 ggttgttcct ccggttggcg ccgttggcct tgccggggcc tgccagccgg ccacaaaccg
       61 gacctgctgg ccggtgctgc gattgagcag taccatcccg tcgcgcgggg tgaagaacaa
      121 ccagttgccc gcctgccggg ccgcgatctt gccggccttg ccggtccagt caccggtcgg
      181 gcttgagccg accagccagc atgcgccatc ggccgggctg gtcggaggac tgttcgcctc
      241 ggcctcgatc gcgccgtgca gcagcgcatc gatccgggcc gctatctcgt tgacatagtt
      301 ttctttctga gattgacccg cgaaaagcag gggcaactcc agccgcaccg tccggctatc
      361 gaacagaggg tcgggcatga tcggatcctt tcaggtttca ggcgatggcc aggctggccg
      421 gcagtgattg tgcgcgatcg ccgatctggc gcacggtgaa gctgccgccg ggcgcggcgc
      481 tggcgagcgc ggcaaattcg gcggcgtcca gttccagcca gggctgcccg gtctgccagg
      541 tggcgagaac ggtggtggca gtgccaaagg tcaccccata ggcttcggtc tgttcgccga
      601 gtgcgacctc cgccccgtct tgccagaacc aagccccccg ggcccgcctg acccaggaca
      661 ggcgggtggt gccggaaggc agcgtttcca gtcgcaggtg gaccgggctg agcggtcgcg
      721 caccgatccc tcgcagcgcg atcggcgcgg tgaccggggc cgtatcgcca agaccaagcg
      781 ctgcgaccag cgtatcggga acctgcccga ccagcgcggg atcgagctgg gtccccgaac
      841 cgtcgagcaa gacaaagctc tcgccgctgg catggctggc cacggcctgt tcggtgccgc
      901 cgcgcccacg ccacagcccc gacaggtgcc actggctcgc gctcaattga accgcctcgg
      961 cgaactggat gatctcgctg cccagcagcg cgcggttggc gcccatcgca agctggcgca
     1021 tggtggcgtt gcccatcaca agatcgggcg caaccagttg aatggtaacg ctgttgtgac
     1081 ggtcgaacag caatggcgtc gcactgccca gaccggtgat ggccgtaccc agcaccgcgc
     1141 ggtcgcggcc gcttgacccc accggaagca gcgagccatc accctgatcg acaaagaggc
     1201 tggccccgcc ccagctgctg ccaggcgatg agcaggcggc caggatcaac gggaccggag
     1261 tggctggatt gccatcccag ggcagttcgc aggcggccag gctggtggtg gccagcgccc
     1321 cgtcgagcgg cggattggtc cgccccggat cggccggcgc ggcggggagt tcgagtgttg
     1381 gcgacaggcg tgcgagcgaa aggtcgatcc cctgttcgtg ccactcccat tcgcgcaccc
     1441 gccagagccc ggcatgattg ggaagcgaaa caaccgcgcc cgggcgaacc gccggatcga
     1501 tctgggtcac gcgccaggat atggtctggc gtgcccagtt gatccgcttg gccgcgcctt
     1561 ccaccagctg gcgcgcagcc gcagcgttga gcgcggcggg cagttcgatc gtgcgcggct
     1621 ctccgggatg agggcgcccg gcggcgcgct gcgcgccagg ctggtaatcg cgctcgacat
     1681 cgtaataacg cagtaccgcg atcggagctt cgggttgggc gccgcgcttg cgggcaaaac
     1741 ccgcattgcc gccaaaatcc tcgcgccggg tcgatgtggc ggcttcgggc agggcaatga
     1801 ccgcggcctg attgcgatcg gggcggattg ccaggcgttc gtcgcaggca tcgcaatcga
     1861 ccgggaacag cggatcgagc acgcccagca gttcgccaac cgatccgtca caggtcagcc
     1921 cggtcaggcc cggcaggggc acgtcggcat cgatatcgtc gaccgcgccg acaaacaccg
     1981 gggcgaggcc gagcgggccc ggatcggcga tcacttcgaa cgacagcgtg gggatgcggt
     2041 tgccgtattc cgccagctgc aggtcttcga acaccacata ggcaaggttg cggtaggccg
     2101 ggcacaggct tgcgccctct gccgcgacca gcagcggatc ggccccctga tcgccgcggc
     2161 ctgaatgcag ccgcattgtc ccggcaacct tgagatcgcc ggccgcgccg cgcagcagct
     2221 tgccatcggc ccagatccgg ccgatcgccc caaccgggcg cgaagacagc gcaaccgcaa
     2281 acgacgccga ataggtgtag gttactaccg acgggcggcc cttgccgccg ccctgctttt
     2341 ccttgtgctc gaccaggtcg gttgcccaga tgatctgccc gggcacacgg atagttccga
     2401 agtggcgggg gatcggcatg ccatagcttg acgtggtgac cgacagctcc ttgagccgtg
     2461 gcccttcacg gctgccaccg cggccgaaga tcatcgaatc gacctggcgt ccgaccatcg
     2521 atccgatcgc accgccaagc gggccgccga gcatggtgcc gacgactgtg agaaccaggg
     2581 ttgccatggc tgagactttc aggggttggg cagcaggcgc cagtgatggg tctgtgttcc
     2641 ggcgggcaga ccgggctcgc gcaccacccg ccgcaatccg gcatgggcgt ggatccaggc
     2701 tccgtccgcc gctgcaatcg cgaggtggaa ttgcgctggc ccgggcgcca gcagcaccac
     2761 gtcgcccggg acaaaggggc gctcggcaac g
//
LOCUS       DASUDV010000238         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1487671,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000238 DASUDV010000000
VERSION     DASUDV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487671"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFV30_05120"
     CDS             complement(<1..309)
                     /locus_tag="VFV30_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847501.1"
                     /translation="MRKFATAVIAATALLAAPSLLAKPRLTGEERLAKRLEGREAGKP
                     QSCLPSWETRDLEIIDGVALVYGHGDTIWVNRPDNAKDLDDDEILVTKTTGSQLCRLD
                     I"
     gene            complement(394..1827)
                     /gene="tldD"
                     /locus_tag="VFV30_05125"
     CDS             complement(394..1827)
                     /gene="tldD"
                     /locus_tag="VFV30_05125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX4847502.1"
                     /translation="MTATDPRSLLYRQLDPAQAQAITREALAACDDGELFLQFTASET
                     FAFDDGRLKTADYSREAGFGLRGVSGEMTGFAHANELSADAIRRAGQTLQLLDPARGQ
                     AAPAPRASNRHLYTDASPLDGLDFAAKVKLLEAIDAAARARDPRVAQVTASLAASWSV
                     VEIVRADGFVATDVRPLVRLNVSIVAESNGRREVGSFGIGGRRLYDDLFQPETWNRAI
                     DSALAQALVNLESVDAPAGEFQVLLGPGWPGVLLHEAVGHGLEGDFNRKGTSAFSGRI
                     GERVAASGVTVVDDGTLLGVNGQGRRGSLSIDDEGTPTQETVLIEDGILKGYMQDRLN
                     ARLMGVPATGNGRRESYAHAPMPRMTNTFMKAGNDDPAELLSRMKNGIFAKSFGGGQV
                     DIVSGKFVFSCTEAYKVENGKLGAPIKGATLIGDGPTVLTKVSGIGNDLAIDEGIGIC
                     GKGGQSVPAGVGQPTLLVEGLTVGGTA"
     gene            complement(1859..2056)
                     /locus_tag="VFV30_05130"
     CDS             complement(1859..2056)
                     /locus_tag="VFV30_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX4847503.1"
                     /translation="MTSTSETVITATTRVSCDGASDIPGGAALGHPRVWLEIDESGEA
                     TCGYCDKRFVLKGGPADQAAA"
     gene            2132..>2707
                     /locus_tag="VFV30_05135"
     CDS             2132..>2707
                     /locus_tag="VFV30_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4847504.1"
                     /translation="MELPPAISIRNLVKRYAPAGNAPAKLALDDVSFDVPQGQIFGLL
                     GPNGAGKSTLINTLAGLVMKTSGEAKIWGFDIDTDSRNAKRSIGIVPQEVVFDPFFTP
                     LEVLEIQAGLYGVPKAARRSLELLGQVHLSDKANAYARTLSGGMKRRLLIAKALVHAP
                     PVIVLDEPTAGVDVELRRQLWDLVSELNAGGV"
BASE COUNT          455 a          895 c          877 g          480 t
ORIGIN      
        1 gatatcgagc cggcacagct ggctgccggt ggtcttggtg acgaggatct cgtcgtcatc
       61 aaggtccttg gcattgtcgg gccggttgac ccagatcgtg tcgccatggc catagaccag
      121 cgcgacccca tcgatgattt ctagatcgcg cgtttcccag ctgggcaggc acgattgcgg
      181 cttgcctgct tcgcgccctt cgagccgctt ggccaggcgt tcctcgccag taaggcgggg
      241 ttttgcgagc agcgatggcg cggcaagcag ggcggttgca gcgatcacag cagttgcgaa
      301 tttgcgcatg agccagattc cttgtctctc cgccccccgg agatgtttca ccgtatcatg
      361 ccaacctgaa cagggcctga aaagccctgt ttcctaggcc gttccgccca cggtcagccc
      421 ttcgaccaag agagtgggct ggccgacgcc cgctggcacc gattgcccgc ccttgccgca
      481 gatgccgatc ccttcgtcga tcgccagatc gttgccgatt ccgctcacct tggtcagcac
      541 ggtcggcccg tcgccgatca gcgtcgcgcc cttgatcggc gcgccaagtt tgccgttctc
      601 gaccttgtag gcctcggtgc aggagaacac gaacttgccc gagacgatat cgacctgccc
      661 cccgccaaag ctcttggcga agatcccgtt cttcatccgg ctgagcagtt cggccggatc
      721 gtcgtttccg gccttcatga aggtgttggt catccgcggc atcggcgcgt gagcatagct
      781 ttcacgccgc ccgttgccgg tggcgggcac ccccatcagc cgggcgttga gccggtcctg
      841 catgtagccc ttgaggatcc cgtcctcgat cagcacggtt tcctgggtgg gcgtgccttc
      901 atcgtcgatc gaaagcgagc cgcgccgccc ctggccattg acgcccagca gcgtgccatc
      961 gtcaaccaca gtcaccccgc tcgcggccac gcgttcgccg atgcggccgg aaaaggcact
     1021 ggtgcccttg cggttgaaat caccttccag cccatggccg accgcctcgt gcagtagaac
     1081 accgggccag ccagggccca gcagtacctg gaattcgccc gccggggcat cgacgctctc
     1141 aaggttgacc agggcctggg ccaaggcgct gtcgatcgcg cggttccagg tttcgggctg
     1201 gaacagatcg tcgtaaagcc gccgcccgcc gatcccgaag ctgcccactt cgcgccgacc
     1261 attgctttcc gcgacgatcg acacgttgag ccgcaccagc ggccgcacat cggtggccac
     1321 gaagccatcg gcgcgcacga tctccaccac cgaccatgag gcggcaaggc tggctgtaac
     1381 ctgggcgacg cgcggatcgc gggcgcgcgc ggcggcatcg atcgcctcga gcagcttgac
     1441 cttggcggca aagtccagcc cgtcgagcgg cgaagcatcg gtatagaggt ggcggttcga
     1501 cgcgcgcggc gcaggcgcgg cctggccccg agcgggatcg agcagctgca gcgtctgccc
     1561 ggcgcggcgg atcgcatcgg cgctcagttc gttggcatgg gcaaagccgg tcatctcgcc
     1621 cgaaaccccg cgcaggccaa agccagcctc gcgcgaatag tcagccgttt tcagccgccc
     1681 atcgtcgaag gcaaaggttt ccgaagcagt gaactgcagg aacagctcgc catcgtcgca
     1741 ggcagcgagc gcctcgcggg tgattgcctg ggcctgggcc ggatcgagtt ggcggtaaag
     1801 cagcgaacgg ggatcggttg cggtcatgca accgatatag ggcattctcg gcgcttgact
     1861 aggccgcggc ctgatccgcc ggtccgccct tcagcacgaa gcgcttatcg caatagccgc
     1921 aggtcgcctc gccgctttcg tcgatttcca gccagacgcg cggatggcca agcgccgccc
     1981 cgccggggat gtcgctggcg ccgtcgcacg agacgcgggt ggtggcggtg atcacggttt
     2041 cgctggtgct ggtcatggcc tgcccgttag caagtggaag ccgcgatttc cagcctttac
     2101 gaaagcggga ttgtgcagca agagcggggc gatggaactg cctcccgcga tctcgatccg
     2161 caacctggtg aaacgctatg ccccggccgg caacgccccg gccaagctgg cgctggacga
     2221 tgtcagcttc gacgtgccgc aagggcagat ctttggcctg ctgggcccga acggcgcggg
     2281 caaaagcacg ctgatcaaca cgctggccgg gctggtgatg aaaacctcgg gcgaagcgaa
     2341 gatctggggc ttcgatatcg ataccgacag ccgcaacgcc aagcgttcga tcgggatcgt
     2401 cccgcaggaa gtggtgttcg atccattctt caccccgctt gaagtgctgg aaatccaggc
     2461 tgggctctac ggtgtgccca aggcggcgcg acgcagtctg gaactgctgg gccaggttca
     2521 cctgtccgac aaggccaacg cctatgcccg cacgctttcg ggcgggatga agcgccggtt
     2581 gctgatcgcc aaggcgctgg tccacgcccc gccggtgatc gtgctcgacg agccgaccgc
     2641 gggcgtcgat gtcgaactgc gccggcagct gtgggatctg gtgagcgaac tgaacgccgg
     2701 aggggtc
//
LOCUS       DASUDV010000239         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1355429,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000239 DASUDV010000000
VERSION     DASUDV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355429"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..839)
                     /locus_tag="VFV30_05140"
     CDS             complement(135..839)
                     /locus_tag="VFV30_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4847505.1"
                     /translation="MSTLERIDYADGETALTGWLVRPAGSARAAVAVYPTFMNKTPGV
                     EAKARALAEHGYTAFIADFYGPDSPHDFDSANSAMIKLRADPRAMRQRLRATLAKLAE
                     LEPGLPQAAIGFCLGGMAVLELARDGADLALVASFHGLLQSPLPATAPIKPRILVCHG
                     DVDFLVPRSHVTAFWEEMDSVKANWHFHSYAGVDHGFTNPLTPAGAPNPSYNASADRQ
                     SWSALLGLLDEVLPAT"
     gene            complement(836..1753)
                     /gene="cysK"
                     /locus_tag="VFV30_05145"
     CDS             complement(836..1753)
                     /gene="cysK"
                     /locus_tag="VFV30_05145"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX4847506.1"
                     /translation="MKADSILATIGNTPHIRLSRLFPDHEVWVKAERANPGGSIKDRI
                     GLAMIEAAEADGSLKAGGTIVEPTSGNTGIGLAMAAAVKGYKLVLVMPESMSIERRRL
                     MLAYGATFDLTPREKGMKGAIERAHELVASTPGAWMPQQFENPANIAVHVRTTAQEIL
                     ADFADTPIDALITGVGTGGHLTGCAEALKAKWPGMKAWAVEPTLSPVISGGQPSPHPI
                     QGIGAGFIPANLHTQAIDGAIQVDPADAKEMARRCAREEGMLVGISSGATLAAIAQKL
                     AELSAGSRVLGFNYDTGERYLSVPDFLPE"
     gene            complement(1750..3015)
                     /locus_tag="VFV30_05150"
     CDS             complement(1750..3015)
                     /locus_tag="VFV30_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847507.1"
                     /translation="MSAASHPFQIRDYRLFWIARFAAVAATMAMVVVLAAQTYLIARR
                     EYGMAKDEAAFLIGLLGLAQFLPLLLLTPVAGWAADRFDRRRLAALANLLDLGVCLLL
                     ALANMAGVLTLPLLFAMAAAHGAARVFVGPAMSSIVANVVPAEVLPRAVAFNSIAWQS
                     ASVIGPAVGGFLLARSGDAPYWFGVVLLALASVAVIAIRPFPKPPQSNAHPLRQMADG
                     ARYVWHERFLLGCISLDLFAVLLGGATAMLPAFAYDVLHVGDEGLGLMRAAPAAGAAL
                     VAVWLGFRPLHHNVGVKMLWSVVVFGAATLAFGLSNNFLLSLGLLALLGGADMVSVFI
                     RGTLVQLNTPDEMRGRVSSISGLAISASNELGEMQSGLAAAALGATGAVIFGGVGAIV
                     VTAIWAVLFPELKNAKTFAPQYRNKEPAP"
     gene            complement(3086..>3236)
                     /locus_tag="VFV30_05155"
     CDS             complement(3086..>3236)
                     /locus_tag="VFV30_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4847508.1"
                     /translation="MPEVGRIEAHLIWASEGRAGFQFERIIRLEDFMRMIEALQPNPR
                     LRRSR"
BASE COUNT          583 a         1041 c         1107 g          505 t
ORIGIN      
        1 cgcgcgctgc gccagggcat agggtagggc gaagatgccg gccccgatca cgccgttgat
       61 gaccaatgcg cacaggccgg cgagcccgac gcccctgatc agccctgtct gtccccccat
      121 ttgccccccg ggtgctaagt cgctggcaat acctcgtcga gcaggcccaa cagtgccgac
      181 cacgattggc gatcggcgct ggcgttgtag ctgggattgg gtgcgccggc aggggtcagc
      241 ggattggtga acccgtgatc gaccccggca tagctgtgga agtgccaatt ggccttcacg
      301 ctgtccattt cctcccagaa agcggtgacg tggctgcgcg gtacgaggaa atcgacatcg
      361 ccgtggcaaa ccaggatgcg cggcttgatc ggggctgtag ccggcagggg cgattgcagc
      421 aggccgtgga agctggcgac gagggcgagg tcggccccgt cgcgcgccag ttcgagcacg
      481 gccatgccgc caaggcagaa cccgattgct gcctgcggca gacccggttc gagctcggcc
      541 agcttggcga gggtggcgcg tagccgctgg cgcatagcgc ggggatcggc gcgcagcttg
      601 atcattgcac tattggcgct gtcgaagtcg tgcggcgaat ccgggccgta gaaatcggcg
      661 atgaaggccg tgtaaccgtg ctcagccagt gcccgcgctt tggcctctac cccgggagtc
      721 ttgttcatga aggtcggata gaccgctacg gctgcgcgcg cactcccggc cgggcggacg
      781 agccagccgg tcagggctgt ctcgccgtcc gcgtagtcga tccgctccag cgtgctcatt
      841 cgggcaggaa gtccggtacc gaaagataac gctcaccggt atcgtagttg aagccaagca
      901 cgcggctgcc ggcagacagt tcggccagct tttgggcaat cgcggccagg gtcgcgcccg
      961 agctgatgcc gacgagcatg ccttcctcgc gcgcgcagcg gcgggccatt tccttggcat
     1021 cggccggatc gacttgaatc gcgccgtcga tcgcctgggt gtggaggttg gccgggatga
     1081 agcccgcccc aatcccctgg atcggatgcg gcgagggttg cccgcccgag atcaccggcg
     1141 aaagcgtcgg ttcgaccgcc caggctttca tgccgggcca cttggccttg agggcctcgg
     1201 cacagccggt caggtgcccg ccggtgccaa ccccggtgat cagcgcatcg atcggggtat
     1261 cggcgaaatc ggcgagaatt tcctgagccg tggtgcggac atggaccgcg atgttcgcgg
     1321 ggttttcgaa ctgctgcggc atccacgcgc cgggggtcga ggcgaccagt tcgtgcgcgc
     1381 gttcgatcgc gcccttcatg cctttttcgc gcggggtaag gtcaaaggtc gcgccatagg
     1441 ccagcatcag ccggcgccgc tcgatcgaca tcgattcggg catgaccagc accagcttgt
     1501 aacccttgac cgcggcggcc atggcgaggc caatcccggt gttgccgctg gtcggctcga
     1561 caatcgtgcc gcccgccttc aggctgccat cagcctcggc cgcctcgatc atggcaagac
     1621 cgatccggtc cttgatcgaa ccgcccgggt tggcgcgttc tgccttcacc cacacttcgt
     1681 gatcgggaaa caggcgcgaa aggcggatgt gcggggtgtt gccaatcgtg gcgaggatcg
     1741 aatcggcctt catggggcag gctccttgtt gcgatattgc ggggcaaagg ttttggcgtt
     1801 cttgagttcg ggaaacagca cggcccagat tgcggtgacg acgatagcac ccacgccgcc
     1861 gaaaatcacc gccccggttg cgcccagcgc cgcggcggca aggcccgact gcatttcgcc
     1921 cagttcgttc gaggcggaga tcgccaggcc cgagatcgag gatacgcgtc cgcgcatttc
     1981 atcgggtgtg ttgagctgca ccagggtgcc acggatgaac accgaaacca tgtcggcgcc
     2041 gcccagcagg gccagcaggc caagcgaaag caggaagttg ttcgacaggc cgaaggccag
     2101 cgtcgcggcg ccgaatacca ccactgacca cagcatcttc actccgacat tgtggtgcag
     2161 ggggcgaaag cccagccaga ctgcaaccag cgcggcgccg gcggcgggcg cggcgcgcat
     2221 cagccccagg ccctcatcgc ccacgtgcag cacgtcatag gcgaaggcgg gaagcatggc
     2281 agtggcgccg cccagcagca cggcgaacag atcaagcgag atgcagccaa gcagaaaccg
     2341 ttcgtgccac acatagcgcg caccatcggc catctggcgc aatgggtggg cattgctttg
     2401 cggcggcttg gggaaggggc ggatcgcgat caccgccacc gatgccaggg ccagcaggac
     2461 cacgccgaac cagtacgggg catcgcccga gcgggcgagc agaaacccgc ccaccgctgg
     2521 accgatcacc gatgcgcttt gccaggcgat cgaattgaat gccaccgcgc gcggcaggac
     2581 ttcggccggg accacgttgg caacgatcga actcatcgcc ggccccacga aaacccgggc
     2641 cgcgccgtgg gccgctgcca tggcaaacag cagcggcagg gtcagcacgc cggccatgtt
     2701 ggcaagggcc agcagcaggc aaactccaag atcgagcaga ttggccagcg cggccagccg
     2761 gcgccggtca aagcggtcgg ccgcccagcc ggccaccggg gtgagcagca gcaggggcag
     2821 gaattgcgcc agtcccagca ggccgatcag aaacgcggcc tcgtccttgg ccatgccgta
     2881 ttcgcgccgg gcgatcagat aggtttgggc cgccagtacc acgaccatgg ccatggtggc
     2941 cgccaccgcc gcgaagcggg cgatccagaa caggcggtag tcgcggattt gaaagggatg
     3001 actcgcggcg ctcacggcca aggcatggca gcgccgaccg tgattgccaa ggcgtcagaa
     3061 gcttgcttgt gggaaagcgg tgctttcagc gcgaacggcg caggcgcgga ttgggctgaa
     3121 gggcctcgat catgcgcatg aagtcttcga ggcggatgat ccgctcgaac tggaagccgg
     3181 cgcgtccttc gctggcccag atcaggtgcg cctcgatccg gcccacttcg ggcatg
//
LOCUS       DASUDV010000240         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_162629,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000240 DASUDV010000000
VERSION     DASUDV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162629"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFV30_05160"
     CDS             complement(<1..571)
                     /locus_tag="VFV30_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX4847509.1"
                     /translation="MVQSRLFALIALAIPLSAFGARYPENTWLQVGPVALLLPAAWWA
                     LRRYPISDAAAGCLAAFVLLHLFAARWSYSFVPYDHWLPGLGSAFGFERNMFDRLVHF
                     LFGVLAVLPFAEIATRHGGLSLRAALWAAMAFVLAVGGLYEIFEWTLTLALAPEDAGA
                     YNGEQGDRFDAQKDMAIAAFGAILALIPVR"
     gene            complement(728..1753)
                     /locus_tag="VFV30_05165"
     CDS             complement(728..1753)
                     /locus_tag="VFV30_05165"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX4847510.1"
                     /translation="MGYRVVVVGATGNVGREMLNVLAEREFPIDELAALASSRSTGDE
                     IEFGETGKMLKVRNIEHFDFAGWDIALFSAGSEVSKVFAPKAAAAGCVVIDNSSFYRM
                     DPDVPLIVPEVNPDAIDGYKAKMIIANPNCSTAQMVVALKPLHDAAKIKRVVVATYQS
                     VSGAGKQGMDELFEQSRNIFVGDSSEPVKFTKQIAFNVIPHIDSFLDDGSTKEEWKMV
                     VETKKILDPKIKVTATCVRVPVFVGHSEAINLEFEDEISAEQAQDILREAPGVMLVDK
                     REDGGYVTPVECVGDGATFVSRVREDPTVESGLSLWCVSDNLRKGAALNAVQIAELLG
                     RRHLKKG"
     gene            complement(1792..2406)
                     /locus_tag="VFV30_05170"
     CDS             complement(1792..2406)
                     /locus_tag="VFV30_05170"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEX4847511.1"
                     /translation="MTLLTRRAVLAGGAGLALAGCGAATAQSRPAAPRALHLIQAARG
                     QVGVTLRYDPAYTRIGYPGGDVPRAKGVCTDVVIRAYRDALAIDLQSEVHGDMAANFA
                     RYPRRWGLRRPDRNIDHRRVPNLETFWTRQAARLPIPARPGDWRPGDLFTALIDGRLP
                     HTGIVSDRIAANGNPLVIHNIGAGTREEDMLFAHRLTGRFRWRL"
     gene            complement(2403..4577)
                     /locus_tag="VFV30_05175"
     CDS             complement(2403..4577)
                     /locus_tag="VFV30_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX4847512.1"
                     /translation="MSASRIAAPQLSFERVFASPSLNGAVPRGVKLSPDGRWLTVLRS
                     RADDRERYDLWGFDRTTGQWVMLVDSLKLGSGKDLSEAEKMQRERQRIGDLKGIVNYE
                     WTADGSAILVPLDGDLFLARLDGSVRKLTESKEDELNPALSPKGAYVSFVRDRRLWAG
                     KVGEEPRPVTPEEAAETVHWGEAEFVAQEEMGRFTGYWWAPDDSRIAVQRFDDAPVGI
                     VTRTAIGAEGTKTFQQRYPVAGSANSLVSLFVMAPDGSNRVEVDLGPNRDIYLARVNW
                     SPDGKVLYVQRQNREQTVLEMLAVDPATGKAKVLFTEKAAPRSWINLSNDYRFLKDGS
                     LVWWSERDGYGHLWHFKDGKWKQLTKGSWVVQSLVGIDEKTGRVYFTANRDDVLAANV
                     YALNLSSPGKVERLGEPGYVHAAAMDKAGQTLLITRSSPSQPPQVYIADAKGKRLAWV
                     EENRIDAKHPYAPFLAAHRAPTFGTIKAADGTELHWRMITPPLVPGKRYPVFFSHYGG
                     PTSQVVTRGWGGGLEQAIVAKGYIHFQIDNRGSENRGVAFASTLYRAMGGVEVEDQKA
                     GALFLKTLPQVDGDRIATYGWSYGGYMTIKMLQADPGLYAAGIAGAPVTKWELYDTHY
                     TERYLGDPNKEPKVYETANALADAGKIADPLLLIHGMADDNVVFENTSMLVSRMQAQA
                     VPFEMMLYPGYTHRVGGPKVGVHLWETIFAFLDKNMPERSAK"
     gene            complement(4748..>5941)
                     /locus_tag="VFV30_05180"
     CDS             complement(4748..>5941)
                     /locus_tag="VFV30_05180"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX4847513.1"
                     /translation="MAFFADFRTMGWSQADQQVWLFLETFWEGTQRGMLELLFGAGLM
                     VTAAKAMEHDGPVGVADLYIRRNLWLLLFGLIDIFALLWFGDILLIYALCALALFPFR
                     KLAVRWLLVMGLALATFMAVIGGIEYVSRGELQETHTVAVEKQQSGQKLTGEEAEAIK
                     KWDTIIARATGKEPEMQKLAKDEVAARAGSFGDYAEFLTSTYVLIVSKGSLLGGVAEA
                     FSVMLIGIALWKLGFIQGQRSTRDYVIVLILAYGFGLTARYVGGLERMTFLPVPKTLW
                     MTQEYARIAVSLGHVALINLLMRFAPGRAALKPFIAAGQMAFSLYFLQQFIGIYVLYS
                     PLGLHLPGGQGWAHTSALAGGIVIGLLIFANLWMRLFASGPLEWLWRSLSYWRRQPFR
                     RIAAR"
BASE COUNT         1038 a         2025 c         1897 g          981 t
ORIGIN      
        1 ggcgcacggg gatcagtgcc aaaattgcgc cgaaggccgc gatcgccatg tccttctggg
       61 cgtcgaaacg gtcgccctgc tcgccgttgt aggccccagc atcctccggc gcgagggcca
      121 gggtcagcgt ccattcgaag atctcgtaaa gcccgccgac ggccaggacg aaggccatgg
      181 ctgcccacag cgccgcccgc aggctcagcc caccgtggcg cgtcgcaatc tcggcaaagg
      241 gcagcacggc gaggacgccg aacaggaaat ggaccagccg gtcgaacatg ttgcgctcaa
      301 acccgaacgc ggaccccagg cccggcagcc agtggtcata gggcacaaag ctgtaggacc
      361 aacgcgcggc gaacaggtgc agcaggacga aggcggcaag acagcccgcc gccgcatcgg
      421 agatcggata gcgccgcaag gcccaccagg cggccggcag caacagcgcg accgggccga
      481 cctgcagcca ggtgttttcg ggatagcgcg cgccaaaagc gctgagcggt atcgccagag
      541 cgatcagcgc gaagaggcgg ctctggacca tggccaaagc ttgctcaatc ccggtcggac
      601 ttgcaagcca gtgccgcccc gctattttcg cgtggctctc agccaagccg gcagacttga
      661 cttggcgagg ggatcagctg gggcttcgag agaagcagaa gggtttctgc cgccctccca
      721 gcaccaatca ccccttcttc agatgccgcc gccccagcag ttccgcgatc tgcaccgcgt
      781 tgagcgcggc gcccttgcgc aggttgtcgg acacgcacca cagcgacagg ccgctttcca
      841 cggtcggatc ctcgcgcacg cgggagacga aggtggcgcc atcgccgacg cattccaccg
      901 gcgtgacgta accgccgtcc tcgcgcttat cgaccagcat cacgccgggg gcttcgcgca
      961 ggatgtcctg ggcctgttcg gcgctgatct cgtcctcgaa ttccaggttg atcgcttcgg
     1021 aatggccgac gaacaccggc acgcggacgc aggtggcggt gaccttgatc ttcggatcca
     1081 ggatcttctt ggtttccacc accatcttcc attcttcctt ggtcgatccg tcgtccagga
     1141 agctgtcgat atgggggatc acgttgaagg cgatctgctt ggtgaacttc accggctcgc
     1201 tgctgtcgcc cacgaagatg ttgcggctct gctcgaacag ctcgtccatg ccctgcttgc
     1261 ccgcgccgga caccgattga taggtcgcga cgaccacccg cttgatcttc gccgcatcgt
     1321 gcagcggttt cagcgcgacg accatctgcg cggtcgagca gttggggttg gcgatgatca
     1381 tcttggcctt gtagccgtcg atcgcatcgg ggttcacttc gggcacgatc agcggaacgt
     1441 ccggatccat ccggtagaac gaggaattgt cgatcaccac gcagccggcc gcggcggcct
     1501 tgggcgcgaa gaccttcgac acttcggacc cggcggaaaa cagcgcgatg tcccagccgg
     1561 cgaaatcgaa atgctcgatg ttgcggacct tcagcatctt gccggtttcg ccgaactcga
     1621 tctcgtcccc ggtggagcgc gacgaggcga gcgccgccag ctcgtcgatc ggaaactccc
     1681 gttcggccag cacgttgagc atttcccgcc cgacattgcc cgtcgcaccg acgacgacta
     1741 cccgataacc catggctgaa actccgttgt gttcggcccg cccctagcgg tttagagccg
     1801 ccagcggaag cgcccggtca gccggtgggc gaacagcatg tcctcttcgc gggtcccggc
     1861 gccgatattg tggatcacca gcgggttgcc gttcgccgcg atccggtccg aaacgatccc
     1921 ggtgtggggc aagcggccgt cgatcagcgc ggtgaacagg tcgccgggcc gccagtcccc
     1981 gggccgcgcc gggatcggca ggcgggcggc ctggcgggtc cagaaggttt ccagattggg
     2041 gacccggcgg tgatcgatgt tgcggtctgg tcggcgcagc ccccagcggc ggggatagcg
     2101 ggcgaagttt gccgccatgt cgccgtgaac ctcgctttgc aggtcgatcg cgagggcatc
     2161 gcggtaggcg cggatcacca cgtcggtgca gacgcccttg gcgcgcggca cgtcgccgcc
     2221 cgggtagccg atccgggtgt aggccggatc gtaacgcagc gtgacgccca cctgcccccg
     2281 cgccgcctgg atcaggtgca gcgcgcgcgg cgcggcgggg cggctttgcg cggtggccgc
     2341 gccgcagccg gccagcgcca gcccggcccc accggccagc acggcgcggc gggtcagcag
     2401 ggtcatttcg cgctccgttc cggcatgttc ttgtcgagga aggcgaagat cgtctcccac
     2461 aggtgcacgc ccactttcgg cccgccgacc cggtgggtgt agccggggta gagcatcatt
     2521 tcgaacggca ccgcctgggc ctgcatccgc gagaccagca tcgaggtgtt ttcgaacacc
     2581 acgttgtcat cggccatgcc gtggatcagc agcagcggat cggcgatctt gccggcatcg
     2641 gccagcgcgt tggcggtttc atagaccttg ggctccttgt tcggatcgcc caggtaccgt
     2701 tcggtgtaat gggtgtcgta aagctcccac ttggtcaccg gcgctccggc gatcccggcg
     2761 gcgtaaagcc ccggatcggc ctgcagcatc ttgatcgtca tatagccgcc ataggaccag
     2821 ccgtaggtgg cgatccggtc cccatcgacc tgcggcaggg tcttcaggaa cagcgccccg
     2881 gccttctggt cttccacctc aaccccgccc atcgcgcggt agagggtgga ggcaaaggcc
     2941 acgccgcggt tctcgctccc gcggttgtcg atctggaagt ggatatagcc cttggcgacg
     3001 atcgcctgtt ccagcccccc gccccagccg cgggtgacga cctgcgaggt cggcccgcca
     3061 tagtgcgaga agaacaccgg atagcgcttg cccggcacca gcggcggggt gatcatccgc
     3121 cagtgcagct cggtcccgtc ggcggccttg atcgtgccaa aggtcggcgc gcgatgggcg
     3181 gccaggaacg gcgcataggg gtgcttggcg tcgatccggt tctcttccac ccaggccagc
     3241 cgcttgccct tggcatcggc gatatagacc tgcggcggct ggctgggcga ggaacgggtg
     3301 atcagcaggg tctgcccggc cttgtccatc gcggcggcgt ggacatagcc cggctcgccc
     3361 agccgctcca ccttgccggg actggacaga ttcagggcat agacgttggc cgccagcaca
     3421 tcgtcgcggt tggcggtgaa atagacccgt ccggtcttct catcgatccc gaccagcgat
     3481 tgcaccaccc agctgccctt ggtcagctgc ttccacttgc cgtccttgaa gtgccacaag
     3541 tggccgtagc cgtcgcgttc cgaccaccag accaggctgc cgtccttgag gaagcggtaa
     3601 tcgttcgaca ggttgatcca gctgcgcggg gcggcctttt cggtgaacag caccttggcc
     3661 ttcccggtgg ccggatcgac cgccagcatt tccagcacgg tctgttcgcg gttctggcgc
     3721 tggacataga gcaccttgcc gtcgggcgac cagttgaccc gggcgaggta gatgtcgcgg
     3781 ttcgggccca aatccacttc gacccggttc gatccgtccg gcgccatgac gaacagcgag
     3841 accagcgaat tggccgatcc cgccaccgga tagcgctgct ggaaggtctt ggtcccttcc
     3901 gcgccgatcg cggtgcgggt gacgatgccg accggcgcgt catcgaaacg ctgcaccgcg
     3961 atgcggctgt catcgggcgc ccaccagtag ccggtgaagc ggcccatctc ttcctgcgcg
     4021 acgaattccg cctcgcccca gtggacggtc tcggcggctt cctccggcgt caccgggcgc
     4081 ggttcctcgc cgaccttgcc ggcccacagc cgccggtcgc ggacgaacga gacataggcc
     4141 cccttggggc tcagcgccgg gttgagctcg tcctccttgc tctcggtcag cttgcggacc
     4201 gaaccgtcca gccgggccag gaacaggtcg ccgtccagcg gaaccaggat cgcgctgcca
     4261 tcggcggtcc actcgtaatt gacgatgcct ttcagatcgc caatccgctg ccgctcgcgc
     4321 tgcatcttct cggcctcgga cagatccttg cccgacccca gcttcagact gtccaccagc
     4381 atcacccatt ggccggtggt ccggtcaaag ccccacaggt catagcgctc gcggtcgtcg
     4441 gcgcggctgc gcagcacggt cagccagcgg ccgtcgggcg aaagcttcac cccgcgcggc
     4501 accgcgccgt tcaggctggg gctggcgaag acccgttcga acgagagctg cggggccgcg
     4561 atccgcgacg cgctcacggt ttcgcccacg gatggcgccg cggtaacggc aagcaaggca
     4621 gcagcagcgg aaagcaaggc aaggcgcatg gcaaggcagg atccccaaaa ggaaaaaggc
     4681 gccccggccg aaaccgggac gcctctccta attcgctttc gcgcgtcaac gctagaacgt
     4741 tttcgcgtca gcgcgctgca atgcgccgga aaggctggcg gcgccaatag gacaggctgc
     4801 gccagagcca ttccagcgga ccgcttgcga acaggcgcat ccacaggttg gcgaagatca
     4861 gcagcccgat cacgatgccg cctgccagcg ccgaggtgtg cgcccagccc tgcccgcccg
     4921 gaaggtgcag ccccagcggg gaatacagca cgtagatgcc gatgaactgc tgcaggaaat
     4981 agagcgagaa agccatctgc ccggctgcga taaacggctt cagcgcggcc cgaccgggag
     5041 caaagcgcat cagcaggttg atcagcgcga cgtggcccag cgataccgcg atccgggcat
     5101 attcctgggt catccacagc gtcttgggca cgggcagaaa ggtcatccgc tcaagcccgc
     5161 cgacatagcg cgcggtcagg ccgaagccat aggccaggat caggacgatg acatagtccc
     5221 gggtggagcg ctggccctgg ataaagccca gcttccacag cgcgatcccg atcagcatga
     5281 cgctgaacgc ctcggccacc ccgccgagca gcgaaccctt gctgacgatc agcacatagg
     5341 ttgaagtcag aaactcggca tagtcgccaa acgagccggc ccgcgcggcc acctcgtcct
     5401 tggccagttt ctgcatctcg ggctctttgc cggtggcccg ggcaatgatc gtgtcccact
     5461 tcttgatcgc ctccgcctct tcgccggtca gcttctggcc gctttgctgc ttttcgacgg
     5521 caaccgtgtg ggtttcctgc agctcgccgc ggctcacata ttcaatcccg ccgatcaccg
     5581 ccatgaatgt cgccagggcc agccccatga ccagcagcca gcgcaccgcc agtttgcgga
     5641 acgggaacag cgccagcgcg cagagggcat agatcagcag gatgtcccca aaccacagca
     5701 gcgcgaagat atcgatcagc ccgaacagca gcagccacag gttgcgccgg atatagagat
     5761 cggccacgcc gaccgggcca tcgtgctcca tcgccttggc cgccgtcacc atcagccccg
     5821 caccgaacag cagttcaagc atgccgcgct gggtgccttc ccaaaaggtt tccaggaaca
     5881 gccagacctg ctggtcggcc tggctccagc ccatcgtgcg gaaatccgcg aagaaggcca
     5941 t
//
LOCUS       DASUDV010000241         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_626272,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000241 DASUDV010000000
VERSION     DASUDV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626272"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFV30_05185"
     CDS             <1..231
                     /locus_tag="VFV30_05185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX4847514.1"
                     /translation="LGQPLLLGASRKRMIGALNHEAPAHKRLGGSVALAMKGMEAGVQ
                     LLRVHDVPETVQARDVWQGLRDAALTDFGQLA"
     gene            complement(228..728)
                     /locus_tag="VFV30_05190"
     CDS             complement(228..728)
                     /locus_tag="VFV30_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847515.1"
                     /translation="MPRKAILAAAALLLAAPALAQRAAPAPAGTIAVGQVLNDTLGKV
                     VNGWTHNVGAMFKRYETRDGVTTGTLDCCVAVFSRGSSYIVARTEPLARTANGGVIRE
                     KVIAVQRLDLRPGEIETTCDLFNLDIAMSVRNPKTNWVRSVVISYGEIQMLEWRDTSN
                     RCGEES"
     gene            complement(718..2091)
                     /locus_tag="VFV30_05195"
     CDS             complement(718..2091)
                     /locus_tag="VFV30_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEX4847516.1"
                     /translation="MIARFARVLALPLALALSACSAEEAPEPKKADEFALALAVAPEQ
                     EGKLGRVVLPPAALAELKRTDLGDVRIFDAKGRKLSLALGVDKSGQSSTLKANTLPAI
                     PIAAGDKAVGGDVKVEVKAGDATVEVTASDAAMAEDQRVSVLIDTRALSLPVAAIDID
                     ATLPRQVPVTFTLEWSADLKGWVPLADKVLLRPGSDPDLLGTARVALPAVSLRDRYVR
                     ISWQAAPEIAVKGATVFEAVERQPGRVDIDTSGAALTSPHEMRFAPQIVAPISAFKLE
                     MTGPDGVVPVELYGRNDPSQPWGLLAKATLEQGGGAATLELSGATLREYRVVADPRSP
                     GFSKVPKVVIAVEPVVLLAAFNGQGPFNLAVGHANAKPAYFDPADLGKPGELQRAWRR
                     PAEVATAGDAPVILLAPAAPEPAFDPRKWALWGALLLGAAVLGFAAWRLARAGSAAPK
                     KEEADAA"
     gene            complement(2091..>4831)
                     /locus_tag="VFV30_05200"
     CDS             complement(2091..>4831)
                     /locus_tag="VFV30_05200"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX4847517.1"
                     /translation="FGAIFGAGMGAWLRSVLKAEVAREVHLHLAGLNLAPAAPEEAEA
                     PLEDWPEPATAPPAPEPVAKPARRPEPADALAQTWPETPPAPAVTAPAATYRDEPNLA
                     EQLIQRTVEWFTGGNTIIRVGLVVLFVGLVFLARLVANAGLFPLEARLATVGLAGAAL
                     LGVGWWKRIERPDFGLHLQGAGVAVMYLTVFAAARIYEVMPPLAAFAFMIVFAGLGAV
                     LAVLQNSRVLALGSFLGGYAVPVLLGGEAETPLGLFTYITVLNLALMGIAWKKSWRVL
                     NLLGFFATFGLATLWGFGAYEDRHFLICEAFLAVSVGIYLATALLYAHNTPGKLGNVA
                     DSTLLFGTALAGFGLQAGLVHDKPYAAAWSALAFGAVYLAVAGWALKRREQGMGLLFE
                     CVLAIGIGFITLAIPLALDVKWTGAAWALEGAGAYWIGARQGRWMPRAFGLALQAVGA
                     FLTLGTLAPAQSAIPLANTGFFGPLLIAVPVLLTAWWLRRAWPHSGSRFARSWEPVEA
                     ALAPPWFLAGFLFAVLACLMEFARILPQATDADMAMPVFAEANQTMLSLLAVLGLMAA
                     ADLFGRRHDWAVASWPGLLSLPVIAYAYLLLLIMGRRVYDLPELAGWVLAFGIHLWLL
                     RRRDRANAARPSRWDGAVHTGGALLLTAMLADALFGLIERASLWDSSWAGVVFLVAAT
                     AMLFALTRWAGRGAPGGVAGLGWPLHPHARAYWWRAGTVLAFATYAGALGAGWFAQGD
                     AAPLPYVPLLNPIDLAVGLAIAALAYRRQVLKTANPQPRLAAWIAGNGGLGALALLGF
                     VAVNGIWVRTAHHFMAVPWDYTTLSDPTVLTGFSILWTLIAMGLMLHARRSAQRGAWI
                     SGAALLGVVVAKLLFVDMSQAEGIARIVAFIGVGVLMLLIGYFVPLPPRKGDEEKL"
BASE COUNT          826 a         1716 c         1654 g          635 t
ORIGIN      
        1 ctgggccagc cgctgctgct gggggccagc cgcaagcgga tgatcggcgc gctcaaccac
       61 gaggctccgg cgcacaagcg gctgggcggc tcggtcgcgc tggcgatgaa ggggatggaa
      121 gccggggtcc aactgctgcg ggttcacgac gtgcctgaaa cggtccaggc ccgcgatgtg
      181 tggcaggggc tgcgcgatgc ggcgctgacc gatttcggcc agctcgccta agactcctca
      241 ccacagcgat tgctggtgtc gcgccattcc agcatctgga tctcgccata gctgatcacg
      301 accgagcgga cccagttggt cttcgggttg cgcacggaca tcgcgatatc aaggttgaac
      361 aggtcgcaag tggtctcgat ctcgccgggg cgcagatcga gccgctgcac cgcgatcacc
      421 ttctcacgga tcaccccgcc gttggcggtg cgggccagag gttcggtccg ggccacgata
      481 tagctgctgc cgcggctgaa cacggcgacg cagcaatcga gcgtgccggt ggtcacccca
      541 tcccgcgttt catagcgctt gaacatggcg ccaacattgt gggtccagcc gttgaccacc
      601 ttgcccagcg tgtcgttgag cacctggccg accgcgatcg tacccgccgg ggccggcgcg
      661 gcgcgctggg caagagcggg ggcggcgagc agcagggctg cggcggcgag gatggcttta
      721 cgcggcatcg gcttcctcct tcttcggcgc cgcggagcca gcccgggcca ggcgccaggc
      781 ggcgaagccc agcacggcgg cgcccagcag cagcgcgccc cacagcgccc acttgcgcgg
      841 atcgaaggct ggctcgggcg cggccggggc cagcaggatc accggggcat cgccggcggt
      901 tgccacttcg gccgggcggc gccaggcgcg ttgcagttcg cccggcttgc cgaggtcggc
      961 cgggtcgaaa taggccggct tggcattggc atggccgacc gccagattga acgggccctg
     1021 gccgttgaac gcggccagaa ggaccaccgg ttccaccgcg atcacgacct tcggcacctt
     1081 ggagaagccg gggctgcggg gatcggccac cacccggtat tcgcgcagcg ttgcgcccga
     1141 cagttccagc gtggccgcgc cgccgccttg ttccagcgtc gccttggcca gcaggcccca
     1201 gggctgcgac ggatcgttgc gtccataaag ctccaccggg accacgccgt ccggcccggt
     1261 catttccagc ttgaaggcgg aaatcggcgc aacgatctgc ggggcaaagc gcatctcgtg
     1321 cgggctggtc agagcggcgc cgctggtgtc gatatcgacc cggccgggct ggcgctccac
     1381 cgcttcgaac acggtggcgc ccttgaccgc aatctccggc gcggcctgcc agctgatccg
     1441 gacatagcga tcgcgcaagc tgaccgcggg cagggcgacc cgcgccgtgc ccagcaggtc
     1501 cggatcgctg cccgggcgca gcaggacctt gtcggccagc ggcacccagc ctttcagatc
     1561 cgcgctccat tccagcgtga aggtgaccgg cacctggcgc ggcaaggtgg catcgatgtc
     1621 gatcgcggcc acgggcaggc tcagcgcgcg ggtgtcgatc agcacggaca cgcgctgatc
     1681 ctcggccatg gcggcatcgc tggcggtcac ttcgaccgtc gcgtccccgg ccttgacctc
     1741 aactttgacg tcgccgccca cggccttgtc ccccgccgcg atcgggatcg cgggcagggt
     1801 gttggccttc agcgtgctgg actggcccga cttgtcgacg cccagcgcca gcgacagctt
     1861 gcgccccttg gcatcgaaga tccgcacatc gcccagatcg gtccgcttca gttcggccag
     1921 cgccgccggc ggcagtacga cccggcccag cttgccctcc tgctcgggcg cgacggccag
     1981 ggcgagggcg aattcgtccg ccttcttcgg ctccggcgct tcctcggccg aacaggcgga
     2041 cagcgccagg gccagcggca gggccagaac gcgggcgaaa cgggcaatca tcacaacttc
     2101 tcctcatcgc ccttgcgggg cggaaggggc acgaaatagc cgatcagcag catcaataca
     2161 cccacgccga tgaacgcaac gatgcgggct atgccttcgg cctgtgacat atcgacgaac
     2221 agcagcttgg cgacaaccac ccccagcagc gccgcgccgg atatccacgc cccgcgctgg
     2281 gcgctgcgcc gggcatggag catcagcccc atggcgatca gcgtccacag gatcgaaaat
     2341 ccggtcagga ccgtgggatc ggacagggtg gtgtaatccc acggcacggc catgaagtga
     2401 tgcgccgtcc gcacccagat accgttgacc gcgacaaagc cgagcagcgc cagagcgccc
     2461 agcccgccgt tgccggcaat ccatgccgcg aggcgcggct ggggattcgc ggtcttcagc
     2521 acctgccggc gataggccag ggcggcgatt gccagcccga cggccaggtc gatcgggttg
     2581 agcagcggca catagggcag gggggcggca tcgccctgtg cgaaccagcc tgcgcccagc
     2641 gccccggcat aggtggcgaa ggccagcacc gttccagccc gccaccaata ggcccgggca
     2701 tgggggtgga gcggccagcc caacccggcg actccgcccg gcgcaccgcg cccggcccag
     2761 cgggtcaggg cgaagagcat cgcggttgcc gccaccagga agaccacacc ggcccaggag
     2821 ctgtcccaca gactggcccg ctcgatcagg ccgaacagcg cgtcggccag catggcggtt
     2881 agcagcagcg ccccgccggt gtggaccgcg ccgtcccagc ggcttggccg ggcagcgttt
     2941 gcccggtcgc gccgccgcag cagccatagg tggatgccaa aggccaggac ccagcccgcc
     3001 agttccggca ggtcatagac ccgccggccc atgatcagca gcaggagata ggcataggcg
     3061 atgaccggaa ggctgagcag gcccggccag ctggccacgg cccaatcgtg gcggcggccg
     3121 aacaggtctg cggccgccat caggcccagc accgccagca ggctcagcat ggtctggttg
     3181 gcctccgcaa agaccggcat ggccatgtcc gcatcggtgg cctgcggcag aatccgcgcg
     3241 aactccatca ggcaggccag gaccgcaaac aggaacccgg cgaggaacca cggcggcgca
     3301 agcgcggctt caaccggctc ccagctgcgg gcaaagcgcg atccgctgtg cggccaggcg
     3361 cggcgcagcc accaggccgt caacagcacc ggcacggcga tcagcagcgg gccgaagaag
     3421 ccggtgttgg ctagcggaat ggccgactgc gcgggggcta gcgtgccgag cgtcaggaac
     3481 gcgccgaccg cctgcaaggc caggccaaag gcccgcggca tccagcggcc ctgccgggcg
     3541 cctatccaat aggctccggc cccttccagc gcccaggccg cgccggtcca tttgacatcg
     3601 agcgccagcg ggatcgccag ggtgatgaaa ccgatcccga tcgccagcac gcattcgaac
     3661 aacagcccca tgccctgttc gcgccgtttc agcgcccagc cggcgaccgc cagatagacc
     3721 gcgccaaagg ccagcgccga ccaggccgcg gcatagggct tgtcgtggac cagcccggcc
     3781 tgcaggccga acccggccag cgctgtgccg aacagcaggg tcgaatccgc cacgttgccg
     3841 agcttgcccg gggtgttgtg ggcatagagc agcgcagtgg cgaggtagat gccgaccgag
     3901 accgccagga acgcctcgca gatcaggaaa tgccggtcct cgtaagcgcc aaagccccac
     3961 agcgttgcca gaccgaaggt ggcgaaaaag cccagcaggt tgagtacccg ccagctcttc
     4021 ttccaggcga tccccatcag cgccaggttc agcacggtga tataggtgaa cagccccagc
     4081 ggcgtttccg cttcgccgcc cagcagcacc ggcacggcat agccgcccag aaacgagccc
     4141 agggccagga cccgcgaatt ctggagcacc gcgaggaccg cccccaatcc cgcgaagacg
     4201 atcatgaagg caaaggcggc cagcggcggc atgacctcat agatccgcgc ggcggcgaag
     4261 accgtgaggt acatcaccgc caccccggcg ccttgcaggt gcaggccgaa atccggccgc
     4321 tcgatccgct tccaccagcc gacccccagc aaggccgcgc cggccaagcc gaccgtcgcc
     4381 agccgggctt cgagcgggaa cagaccggcg ttggcaacca gccgcgcgag gaacaccagc
     4441 ccgacgaaca gcacgaccag gccgacccgg atgatcgtgt tgccgccggt gaaccactcc
     4501 acggtgcgct ggatcagctg ttcggccagg ttgggttcgt cgcggtaagt ggccgccggt
     4561 gccgtgacgg cgggggcggg cggggtttcc ggccaggtct gggccagcgc gtcggccggt
     4621 tccgggcggc gggccggctt agccaccggt tcgggtgcgg gcggggcggt cgccggttcc
     4681 ggccaatcct ccagcggggc ctcggcctct tccggcgcgg cgggggcaag gttcaggccg
     4741 gcgaggtgca agtgcacttc ccgcgccact tcggccttca gcacgctgcg cagccaggcg
     4801 cccatcccgg cgccgaagat tgcaccaaat a
//
LOCUS       DASUDV010000242         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_272872,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000242 DASUDV010000000
VERSION     DASUDV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272872"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFV30_05205"
     CDS             <1..286
                     /locus_tag="VFV30_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEX4847518.1"
                     /translation="ASREASQPLRSKTGGSTFKNPAGHKAWQLVDEAGCRGLIHGGAQ
                     VSEKHTNFLLNLGDATSADIEALGEEVRRRVKDKSGVELEWEIQRVGKAK"
     gene            283..1257
                     /locus_tag="VFV30_05210"
     CDS             283..1257
                     /locus_tag="VFV30_05210"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX4847519.1"
                     /translation="MTLPKLHVAVLMGGWSNERPVSLMSGEGVAAALEERGHRVTRID
                     MDRDVARKLADVAPDVVFNALHGTPGEDGTVQGMMDLMGLTYTHSGMVTSVIAIDKEL
                     TKQALVPHGIPMPGGRIVDVAEIHERDPLERPYVLKPVNEGSSVGVAIVTAEGNYGNP
                     ISRDAKGPWQEFDRLLAEPFIRGRELTTAVLGEKALMVTELKPKSGFYDFDAKYTEGM
                     TEHVCPAQIPEEITQACLDIALRCHKLLGCKGCSRTDFRWDDERGIEGLFVLETNTQP
                     GMTPLSLVPEQAKHLGISYGELVEIIIADALADKQGRKDALAEKEKAK"
     gene            1254..2255
                     /locus_tag="VFV30_05215"
     CDS             1254..2255
                     /locus_tag="VFV30_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX4847520.1"
                     /translation="MSARTIKRGGKGVRRTAAAHSKTRKVQTARARTGSVIDTLMGWL
                     PFSEAQLHRIFLAMILGVIAVGLWFAASLAGVPAMAREQVARFSHDAGFELHNVTVRG
                     TDHLNELKVYQIALADRNRAMPFVDVDALRARLLELSWVQDARVSRQLPDTLVIDIIE
                     RKPVAVVQKADRLVLIDPEGRELEVVSAARAKGKLLLKGPGAGRQVQALTALLEAAPA
                     LRPQVASAEWVGNRRWNLTFKTGQLLALPEGGDKAAKALVSFAQLDGRNRLLGGKAVA
                     FDMRAPDRIYFRLPGRTDAIEQPRPAANDSARNDAAEKDPAKPAAAAKPSAAKKETP"
     gene            2255..3535
                     /gene="ftsA"
                     /locus_tag="VFV30_05220"
     CDS             2255..3535
                     /gene="ftsA"
                     /locus_tag="VFV30_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX4847521.1"
                     /translation="MAPPRISRVYGAVNIGSFRISAMVAGLSDTGEMVVLGSGHRAAQ
                     GIKRGYVTDMAAATYAIRDAVERAEKMANTSVSSVWIGCSGAGLASQVAQVEVDIGGR
                     RIEVEDVEHLLVSARDVIQPDGRMVLHAQPAHYTLDGADGVSDPKGLHAERLGVDIHV
                     MLADGAPVRNITEAVQNAHLQVEAIVGSPIAAGHACLTAEERELGVALVEFGGEVTNV
                     SVYAAGMLLGLVTIPLGSGDITDAVASAFGIRRFQAERLKCVHGSAIASPSDHRELIP
                     VNAPGEEATGPTARHADDKNRIPRAELVSVITGQLAVWVDEVGKALKALGFTGQRGQL
                     VVLTGGGSELAGLADFAQGALGKPVRLGKPQHLKGLAEAHATPGFSTLAGLVLYAAAD
                     PIDIRAIGPSYQPIVRYKGFGLVNRIYRAMREYF"
     gene            3670..>4797
                     /gene="ftsZ"
                     /locus_tag="VFV30_05225"
     CDS             3670..>4797
                     /gene="ftsZ"
                     /locus_tag="VFV30_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX4847522.1"
                     /translation="MSINIGPPAVEELRPRITVIGVGGAGGNAIANMIVAGIEGVDFV
                     VANTDAQALNNSVAEHRIQLGTDITQGLGAGARPEVGRAAAEESLDQIDRALEGVHMC
                     FIAAGMGGGTGTGAAPVVAKAARDKGVLTVGVVTKPFLFEGTRRMRAAEAGIEELQKH
                     VDTLIVIPNQNLFLVAKAETTFKEAFQMADEVLQQGVRSITDLMVMPGLINLDFADVR
                     SVMAEMGKAMMGTGEGSGENRALEAAEKAIANPLLDGVSMQGAKGVIISIIGGDDMKL
                     LEVDEAANHIRELVDPNANIIWGSAFNPDLQGKIRVSVVATGIEQSAAQAEEASRSFS
                     LGTSRGPARPAMAAPAAEPLFERAQPEPQPEPEPEPEPVAEE"
BASE COUNT          851 a         1572 c         1668 g          706 t
ORIGIN      
        1 cgccagccgc gaggcttcgc agccgctgcg cagcaagact ggtggttcca ctttcaagaa
       61 ccccgccggg cacaaggcct ggcagctggt cgatgaagcc gggtgtcgcg ggctgatcca
      121 tggcggcgcg caggtcagtg agaagcacac caacttcctg ctcaacctgg gcgatgcgac
      181 cagcgccgac atcgaagcgc tgggcgagga agtgcggcgg cgggtgaagg acaagtcggg
      241 cgtggagctc gagtgggaaa ttcagagagt aggcaaggca aagtgacgct ccccaaactt
      301 catgtcgcgg ttctgatggg cggctggtcg aacgagcggc cggtgtcgct gatgagcggc
      361 gaaggcgtcg ctgcggcgct ggaggagcgc ggccatcgcg tgacccggat cgacatggac
      421 cgcgatgtcg cgcgcaagct ggccgatgtc gcgccggacg tggtgttcaa cgcgcttcac
      481 ggcacccctg gcgaagacgg caccgtccag ggcatgatgg acctgatggg gctgacctat
      541 acccattccg gcatggtcac ctcggtcatc gcgatcgaca aggagctgac caagcaggcg
      601 ttggtgcccc acggcattcc gatgccaggc ggacggatcg tcgatgtcgc cgaaatccat
      661 gagcgcgacc cgctggagcg gccctatgtg ctcaagcccg tcaacgaagg ctcgtcggtc
      721 ggcgtggcga tcgtcacggc cgagggcaat tacggcaacc cgatcagccg cgatgccaag
      781 gggccgtggc aggaatttga ccgtctgctg gccgaaccct tcatccgcgg gcgcgaactg
      841 accactgcgg tgctgggcga aaaggcgctg atggtcaccg agctgaagcc caagagcggc
      901 ttctacgatt tcgacgccaa gtacaccgag gggatgaccg agcatgtctg cccggcgcag
      961 atccccgaag agatcaccca ggcctgcctc gacatcgcgc tgcgctgcca caagctgctg
     1021 ggctgcaagg gctgcagccg caccgatttc cgctgggacg acgagcgcgg gattgagggc
     1081 ctgttcgtgc tggaaaccaa cacccagccg ggcatgaccc cgctgagcct ggtgccggaa
     1141 caggccaagc accttggcat ttcctatggc gagctggtcg agatcatcat cgccgatgcc
     1201 cttgcggaca agcagggcag aaaagacgcg ctggctgaga aggagaaggc gaagtgagcg
     1261 cgcggacgat caagcgcggc ggcaagggcg tgcgccgcac cgccgcggcc cacagcaaga
     1321 cccgcaaggt ccagaccgcc cgggcccgca ccggatcggt gatcgacacc ctgatgggct
     1381 ggctgccgtt cagcgaggcg cagctgcacc ggatcttcct ggcgatgatc ctgggcgtca
     1441 ttgccgtggg gctgtggttt gccgccagcc tggccggggt gccggccatg gcccgcgaac
     1501 aggtcgcccg cttctcgcac gacgccgggt tcgaactcca caacgtcacc gtgcgcggga
     1561 ccgatcatct caacgagctg aaggtctatc agattgccct ggccgaccgg aaccgggcca
     1621 tgcccttcgt cgatgtcgac gcgctgcggg cccggctgct ggagctgtca tgggtgcagg
     1681 acgcgcgggt ttcgcgccag ctgcccgata cgctggtgat cgacatcatc gagcgcaagc
     1741 cggtcgccgt ggtccagaag gccgaccggc ttgtcctgat cgatcccgaa gggcgcgaac
     1801 tggaagtggt ttcggccgcc cgggccaagg gcaagctgct gctcaagggc cccggcgcgg
     1861 gccgccaggt ccaggccctg accgccctgc tcgaagccgc gccggcgctg cggccgcagg
     1921 tggcgagcgc cgaatgggtc ggcaaccgcc gctggaacct gaccttcaag accggccagc
     1981 tgctcgccct tcccgaaggc ggcgacaagg cggccaaggc gctggtctcc ttcgcccagc
     2041 tcgacgggcg caaccgcctg ctgggcggca aagccgtggc ttttgacatg cgcgcgcccg
     2101 accggatcta tttccgcctg cccggccgga ccgacgcgat cgaacagccg aggcctgcgg
     2161 ccaacgacag cgcccgcaac gatgcggccg aaaaggatcc cgccaagccg gcggctgccg
     2221 ccaagccgtc cgcagccaag aaggaaacgc cctgatggcg ccgccccgca tttcccgcgt
     2281 ttacggcgcg gtcaacatcg gttcgttccg gatctcggcc atggtggccg ggctttcgga
     2341 taccggcgag atggtggtgc tcggctctgg ccaccgcgcc gcccagggga tcaagcgcgg
     2401 ctatgtgacc gacatggcgg cggcgaccta tgcgatccgc gacgcggtgg agcgggccga
     2461 gaagatggcc aacacctcgg tctccagcgt ctggatcggc tgttcggggg cagggctggc
     2521 cagccaggtc gcccaggtcg aagtcgatat cggcgggcgc cggatcgagg ttgaggatgt
     2581 cgaacacctg ctggtctcgg cgcgcgatgt gatccagccc gacgggcgca tggtcctcca
     2641 cgcccagccg gcgcattaca cgctggacgg ggcggacggg gtgtccgatc ccaagggcct
     2701 ccatgccgag cggctgggcg tggacatcca cgtcatgctg gcggacggcg cgccagtccg
     2761 caacatcacc gaagccgtgc agaacgcgca ccttcaggtc gaagcgatcg tcggctcgcc
     2821 gattgccgcc ggccacgcct gtttgactgc cgaagagcgc gagctgggcg tggccctggt
     2881 cgaattcggc ggcgaggtga ccaatgtttc ggtctatgcc gcgggcatgc tgctggggct
     2941 ggtcacgatc ccgctgggct cgggcgacat caccgatgcc gtcgccagcg cctttggcat
     3001 ccgccggttc caggccgagc ggctgaagtg cgtccacggc tcggccatcg ccagcccgtc
     3061 ggaccaccgc gagctgatcc cggtcaacgc gcccggcgag gaagccaccg gcccgaccgc
     3121 gcgccacgcc gacgacaaga accgcatccc ccgggccgag ctggtctcgg tcatcaccgg
     3181 ccagctggcg gtgtgggtgg acgaagtggg caaggcgctg aaggcgctgg gctttaccgg
     3241 ccagcgcggc cagctggtgg tgctgaccgg cggtggatcc gaactggcgg gactggccga
     3301 ctttgcgcaa ggcgcgctcg gcaagccggt gcggctgggc aagccgcagc atctcaaggg
     3361 cctggccgag gcccacgcca cccccggatt ttcgaccctg gcgggcctgg tgctctacgc
     3421 cgcggccgat ccgatcgaca tccgcgccat cggcccgagc tatcagccga tcgtgcgcta
     3481 caaggggttt ggcctggtca accggattta ccgggccatg cgggagtatt tttaaaccgt
     3541 ttcgggggca cgaaggccac ggaacggctg tggaaaatgg ttaagcggcc tttgattcgc
     3601 cgaatcgaag ggtgcaagga ttgttacagt cgcacgaaat gcatccgccg tgcggacagg
     3661 agagcgtaga tgagcatcaa tatcggaccg cccgctgtcg aggaactgcg tccccggatc
     3721 accgtgatcg gggtcggcgg ggccggcggc aacgcgatcg ccaacatgat cgtggcgggg
     3781 atcgaaggcg tcgactttgt cgtggccaac accgatgccc aggcgctcaa caattcggtg
     3841 gcggaacacc gcatccagct ggggaccgac atcacccagg gcctgggcgc cggggcgcgg
     3901 cccgaagtgg gccgggcggc agccgaggaa agcctcgacc agatcgaccg cgcgctggaa
     3961 ggcgtgcaca tgtgcttcat cgccgccggc atgggcgggg gcaccggcac cggtgccgct
     4021 ccggtcgtcg ccaaggcggc gcgtgacaag ggcgtgctga ccgtcggcgt cgtcaccaag
     4081 ccgttcctgt tcgaaggcac ccgccggatg cgcgcggccg aggccgggat cgaggagctg
     4141 cagaagcacg tcgacaccct gatcgtgatc cccaaccaga acctgttcct ggtcgccaag
     4201 gcggaaacca ccttcaagga agccttccag atggcggacg aggtgctcca gcagggggtg
     4261 cgctcgatca ccgacctgat ggtcatgccg ggcctgatca acctcgactt tgccgacgtc
     4321 cgctcggtca tggccgaaat gggcaaggca atgatgggca ccggcgaggg cagcggcgaa
     4381 aaccgcgcgc tcgaagccgc cgaaaaggcg atcgccaacc cgctgctcga cggcgtgtcg
     4441 atgcagggcg caaagggcgt catcatctcg atcatcggcg gcgacgacat gaagctgctc
     4501 gaagtcgacg aagccgcgaa ccacattcgc gaactggtcg atcccaatgc caacatcatc
     4561 tggggcagcg ccttcaatcc cgacttgcag ggcaagatcc gggtttcggt ggttgccacc
     4621 gggatcgagc agagcgcggc gcaggccgaa gaggccagcc gctcgttcag cctgggaacc
     4681 tcgcgtggtc cggcccgccc ggccatggcc gcaccggctg ctgaaccgct gtttgagcgg
     4741 gcccagcccg agccgcagcc tgaacccgag ccggagcccg aaccggttgc cgaggaa
//
LOCUS       DASUDV010000243         8780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_516161,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000243 DASUDV010000000
VERSION     DASUDV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516161"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFV30_05230"
     CDS             <1..739
                     /locus_tag="VFV30_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4847523.1"
                     /translation="LLEGFLAAGCERVYITARKREQIAETLAEFEAQYPGKVIGLPGD
                     LSQMDEIKRLADTLAERETRLDVLINNAGAAWGAEFDQFPEAGWDKVMDLNVKSLFFL
                     TQKLHPLLNAAASFERPAKVINIASIDGLRPNPWETYSYQASKAAVIHLTKRMAARLI
                     KDNIVVTCIAPGAYPSEMNKAAARNPEGSARGIPYARVGVAEDIAGGAIYLASRAGDY
                     VVGTTIPIDGGIVNGWIPTEFNDPSGH"
     gene            743..1627
                     /locus_tag="VFV30_05235"
     CDS             743..1627
                     /locus_tag="VFV30_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX4847524.1"
                     /translation="MSRAVRTLAEGIYFGEGPRWHQGRLWFSDFYSSRVCSLSLEGDL
                     RTELELDGRPSGLGWMPDGSLLVVRMERRQVWRRWPDGRFELHADLADHSAFLCNDMV
                     VDGHGRAYVGNFGFDLDAELHARGPESVIADHPQTVLALVQPDGTVSDAAPGERFSFP
                     NGMVITPDGCTLIVGETLGGRLSAFAIGADGTLSDRHDYAPTWPRVPDGICLDATGAV
                     WIANPLAPECARIAPGGEVLDVIETGLPCYACMLGGPEGKHLFMLVAPSSDATEAAKT
                     PLGKVLVAEVDASRAGWP"
     gene            complement(1787..2422)
                     /gene="gmk"
                     /locus_tag="VFV30_05240"
     CDS             complement(1787..2422)
                     /gene="gmk"
                     /locus_tag="VFV30_05240"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX4847525.1"
                     /translation="MASNTLQRRGLMFILSSPSGAGKTTISRMLLDRDSEIALSVSAT
                     TRPMRPGEVDGVHYHFVDQATFDRMVDEDAFYEWATVFGHSYGTPKAQIRAGLKEGQD
                     YLFDIDWQGTQQLYQKDQQDVVRVFILPPSIDELRRRLTGRGTDSPEVIAARMERARA
                     EISHWDGYDYVVVNDDVEDCYTQVCEILHAERMKRARQTGLIGFVRGLMKD"
     gene            complement(2454..2717)
                     /locus_tag="VFV30_05245"
     CDS             complement(2454..2717)
                     /locus_tag="VFV30_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847526.1"
                     /translation="MADNRLIRQIARTNGGKLLNAAIDTLLPVDGSGPGRKTLLGGIA
                     GAVAVRVATRSVPGAIVVGGAVIAKRLYDRKRAKSAQKGKPKA"
     gene            complement(2717..3199)
                     /locus_tag="VFV30_05250"
     CDS             complement(2717..3199)
                     /locus_tag="VFV30_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX4847527.1"
                     /translation="MSETPPDSLIPYDEIVQEALRAVVGRVLGQVVDSGGTLPGDHHF
                     YITFKTGAPGVDIPTHLRERFPDEMTIVLQNKFWDLVVEPELFSVGLSFNQIPAKLVV
                     PFSAITAFVDPAVDFGLQFQAAGTDEDLPEHDPAENDTPAAVAEDGSNVVTVDFGRKK
                     "
     gene            complement(3247..3930)
                     /locus_tag="VFV30_05255"
     CDS             complement(3247..3930)
                     /locus_tag="VFV30_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847528.1"
                     /translation="MFWFSLALAGVLALGFARTFFLQPVFQARPLTVPLVIHGICGTG
                     WFALLVWQALQVRKGNMAGHRTIGGQLGMVLATLAVLSAMWIVALTAFDGKNTGSGLS
                     DQSGLFIQLGTSTWFAVLVVLGFRATRRPDYHKRYMIMASITMLAPAYSRIYRLFAGP
                     GPSVVDSAVIAVPLVAALALHDFRTLRRLHPVTLWAGLGYLAYVAVRLPIAKSDLWTQ
                     TIVPALFGI"
     gene            4071..4664
                     /gene="hisB"
                     /locus_tag="VFV30_05260"
     CDS             4071..4664
                     /gene="hisB"
                     /locus_tag="VFV30_05260"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX4847529.1"
                     /translation="MRTAQIARKTHETDIAVVVNLDGTGAYDVSTGIGFLDHMIEQFS
                     RHSLIDITCHIKGDLHVDQHHTTEDSAIAIGQAISQALGDKAGIGRYGTAYSPMDEAL
                     ARVALDISGRPWLVWNARFTQERLGEMDTELFEHWFQSIAQAVGITLHIELLYGSNNH
                     HICEGIYKGFARAMRQAVELDPRKAGAIPSTKGQLGG"
     gene            4657..5277
                     /gene="hisH"
                     /locus_tag="VFV30_05265"
     CDS             4657..5277
                     /gene="hisH"
                     /locus_tag="VFV30_05265"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX4847530.1"
                     /translation="MAEVLALVDYGAGNLHSVANALKAAGAENVTVTANPDVVRAADR
                     IVLPGVGSFKACAEGLRGIPHLVEAMAERVLVGGAPFLGICVGMQLLATTGVEHGETA
                     GLGWIGGEVRLIERTDPAIKIPHMGWNDVEVTRHAALVEAGEAYFLHSYHFAPEDGHH
                     VAAMTDHGGGLVAAVARDNLVGVQFHPEKSQAYGLALLARFLEWKP"
     gene            5279..6007
                     /gene="hisA"
                     /locus_tag="VFV30_05270"
     CDS             5279..6007
                     /gene="hisA"
                     /locus_tag="VFV30_05270"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX4847531.1"
                     /translation="MIVFPAIDLKAGQVVRLAEGDMDRATVYGDNPAAQALLFAEAGS
                     QFLHVVDLDGSFAGRAENRAAVEAILEAFPGHVQLGGGIRTREAVEGWFDLGVSRVVM
                     GSAALKDPEFVKDMAREFPGGIVVAVDAKDGMVATEGWADVSDVEVVDLARRFEDAGV
                     ASLLFTDIGRDGLLKGVNIDATVGLARRVDIPVIASGGVKGLDDIHVLALHAHEGIEG
                     VITGRALYEGRLDLAAAIAMANRA"
     gene            6004..6765
                     /gene="hisF"
                     /locus_tag="VFV30_05275"
     CDS             6004..6765
                     /gene="hisF"
                     /locus_tag="VFV30_05275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX4847532.1"
                     /translation="MTVRIRVIPCLDVRDGRVVKGVNFVDLKDAGDPVEQARAYDAAG
                     ADELCFLDISASHEGRGTLLDVVRRTAEVCFMPLTVGGGVRSVEDARALLLAGADKVA
                     VNSAAVSRPEVVADIAGKMGSQCVVASVDARRTPRGNWEIFTHGGRKATGIDAVEHAV
                     KLAELGAGELLVTSMDGDGTQAGYDLELTRTIADAVSVPVIASGGVGTLDHLVEGVTK
                     GHASAVLAASIFHFGTHTIAEAHAALRAAGLPARG"
     gene            6840..7016
                     /locus_tag="VFV30_05280"
     CDS             6840..7016
                     /locus_tag="VFV30_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX4847533.1"
                     /translation="MVARIASLVLLAAALSTPALAQAASTAIPDPSGVALFGLGLAGV
                     ILGRHLSRRRPGGE"
     gene            7076..7390
                     /locus_tag="VFV30_05285"
     CDS             7076..7390
                     /locus_tag="VFV30_05285"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX4847534.1"
                     /translation="MDTLARLEATIAARHGGDPDASYVARLLHKGLPEVARKVGEEAV
                     ETAVAALAHEREQVVGEAADLIFHLMVLLRAREIPLADVLAELDRREGTSGIAEKAAR
                     GA"
     gene            7391..7768
                     /locus_tag="VFV30_05290"
     CDS             7391..7768
                     /locus_tag="VFV30_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX4847535.1"
                     /translation="MPIDATQPYDDQNIFAKILRGEIPNRTVYEDEWALAFHDINPQA
                     PCHVLVIPKGAYVSWDDFSAKAGAEEIAGFVRAVGHVARTLGLVEPGYRLLANIGGHG
                     HQEVPHLHVHLFGGRPLGPMLAR"
     gene            7862..8320
                     /locus_tag="VFV30_05295"
     CDS             7862..8320
                     /locus_tag="VFV30_05295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX4847536.1"
                     /translation="MTSQPSPPSGLIEGTLHRFAVRAYFEDTDLSGVVYHANYLRWFE
                     RARSDFVRLLGIDQRAVNESGGGAFAVADLTIRYAAPARLDDAVLIETTCTELGAASC
                     RMHQKAFREDGLLLTEASFRIGFVSPDGRPRRMPDGWRAAFAPIMPEGTR"
     gene            8317..>8780
                     /locus_tag="VFV30_05300"
     CDS             8317..>8780
                     /locus_tag="VFV30_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4847537.1"
                     /translation="MTVLALLNAAAAAPKHLDPLALFLQADIVVQAVMAGLVLASVWT
                     WTIIAGFSLRHARLRKDCDSYERDFWKAPDLDAFQAERGKADIPSARVAAAGIGEWRR
                     STGGKTLDRDGTRQRLSQAMDSAIAMEADKLAERLNFLATVGSVAPFVGLFGT"
BASE COUNT         1420 a         3005 c         2964 g         1391 t
ORIGIN      
        1 gctgctcgaa ggcttcctcg ccgccggttg cgagcgggtc tacatcaccg cccgcaagcg
       61 cgagcagatt gccgaaaccc tggccgagtt cgaggcccag taccccggca aggtgatcgg
      121 cctgcccggc gacctcagcc agatggacga aatcaagcgc ctggccgaca ccctggccga
      181 gcgtgaaacc cggctcgacg tgctgatcaa caacgccggt gcggcctggg gcgcggagtt
      241 tgaccagttc cccgaagccg gctgggacaa ggtgatggac ctcaacgtca agagcctgtt
      301 cttcctgacc cagaagctgc acccgctgct gaacgccgcc gccagctttg agcggccggc
      361 caaggtgatc aacatcgcct cgatcgacgg gctgcgcccc aacccgtggg aaacctattc
      421 ctaccaggcc agcaaggcgg cggtgatcca tctgaccaag cggatggcgg cgcggctgat
      481 caaggacaac atcgtggtca cctgcatcgc gccgggcgcc tatccgagcg agatgaacaa
      541 ggccgcggcc cgcaaccccg aaggctcggc ccgcggcatc ccctatgccc gtgtcggcgt
      601 ggccgaggac attgccggcg gcgcgatcta tctcgccagc cgcgcgggcg actatgtcgt
      661 cggcaccacg atcccgatcg acggcggcat cgtcaacggc tggatcccga ccgagttcaa
      721 cgatccgtcg gggcactgag acatgagccg cgcggtccgc accctcgccg aaggaatcta
      781 tttcggcgag gggccgcgct ggcatcaggg ccggctgtgg ttttcggatt tctactccag
      841 ccgggtctgc tcgctctcgc ttgagggcga tctgcgcacc gaactggagc tggatggccg
      901 cccctcgggc ctgggctgga tgcccgacgg ctcgctgctg gtggtgcgga tggaacggcg
      961 gcaggtctgg cggcgctggc cggacggccg gttcgaattg cacgccgacc tggccgacca
     1021 ttccgccttc ctctgcaacg acatggtggt cgatggccac ggccgggcct acgtcggcaa
     1081 cttcggcttc gatctcgatg ccgagctcca tgcccgcgga cccgaaagcg tgatcgccga
     1141 tcacccgcag accgtgctcg cgctggtcca gccggacggc acggtcagcg atgccgcgcc
     1201 gggcgagcgg ttcagctttc ccaacggcat ggtcatcacg cccgatggct gcaccctgat
     1261 cgtcggcgaa acgctgggcg gacggctttc cgcctttgcc atcggcgcgg acggaacgct
     1321 ctcggaccgt cacgactatg ccccaacctg gccgcgcgtg cccgacggga tctgcctcga
     1381 tgcgaccggc gcggtgtgga tcgccaaccc gctcgccccc gaatgcgccc ggatcgcgcc
     1441 gggcggcgaa gtgctggacg tgatcgagac cggcctgccc tgctacgcct gcatgctcgg
     1501 cgggccggag ggcaaacacc tgttcatgct cgtcgcgccg agttcggatg cgacggaggc
     1561 tgcgaaaacg ccgctgggca aggtgctggt ggccgaagtg gacgcgagcc gggccggctg
     1621 gccgtgatcc cctaaatcct ccctgtgcgc gcagcgcatg gggaggtggc agccctgaaa
     1681 gggctgacgg aggggccatg gcgcaacggc ggaaaagccc ctccgtcagc gctgcgcgct
     1741 gccacctccc catcggctcc gccgacaggg aggagctttt ggaccctcaa tccttcatca
     1801 gcccgcgcac gaaaccgatc agcccggtct ggcgcgcgcg cttcatgcgt tcggcgtgga
     1861 ggatttcgca gacctgggtg tagcaatcct cgacatcgtc gttgaccacc acatagtcgt
     1921 acccgtccca gtggctgatc tcggcccggg cgcgttccat gcgggcggcg atcacttcgg
     1981 ggctgtcggt cccccgcccg gtcaggcggc ggcgcagttc gtcgatgctg gggggcagga
     2041 tgaacacccg gaccacgtcc tgctggtcct tctggtacag ctgctgggtg ccctgccagt
     2101 cgatatcgaa cagatagtcc tgcccttcct tcaggccggc gcggatctgc gccttgggcg
     2161 tgccatagga atggccgaag accgtggccc attcgtagaa ggcgtcctca tcgaccatcc
     2221 ggtcaaaggt ggcctgatcg acaaagtgat agtgcacccc gtccacttcg ccggggcgca
     2281 tcggccgggt ggtggccgat accgacaggg cgatttcgct gtcgcggtcc agcagcatcc
     2341 tgctgatcgt ggtcttgccc gcgcccgacg gcgaggacag gatgaacatc aggccgcggc
     2401 gctggagagt gttggaagcc atgcgtgctg atggcccaac ggcggggggc aaatcaagcc
     2461 ttgggcttgc ccttttgcgc actcttcgcg cgcttgcggt cgtaaagccg cttggcgatg
     2521 accgcgccgc ccaccacgat cgcgcccggt accgagcggg tcgccacccg caccgccacc
     2581 gccccggcga tcccgccgag cagggtcttg cgccccgggc ccgatccatc gaccggcagc
     2641 agggtatcga tcgccgcgtt gagcagcttg ccgccgttgg tgcgggcgat ctggcggatc
     2701 agccggttgt cggccatcac ttcttccgcc cgaaatcgac cgtgacgacg ttggagccgt
     2761 cctcggccac ggcggcgggg gtgtcgttct cggccggatc gtgctcgggc agatcctcgt
     2821 cggtgccggc cgcctggaac tgcaggccga aatcgaccgc cggatcgacg aaagcagtga
     2881 tcgccgagaa cgggaccacc agcttggccg ggatctggtt gaacgacagg ccgaccgaaa
     2941 acagctccgg ctcgaccaca aggtcccaga acttgttctg caggacgatg gtcatttcgt
     3001 cgggaaaccg ctcgcgcagg tgggtcggga tgtcgacccc gggggccccg gtcttgaagg
     3061 tgatgtagaa atggtggtcg cccggcaggg tcccgccgct gtccaccacc tggcccagca
     3121 cccgccccac cacggcgcgc agcgcctcct gcacgatttc gtcgtagggg atcaggctgt
     3181 cgggcggggt ctcgctcatg gagggacaag tggcctcgca tggggccggg gtcaagtgcc
     3241 gaacggtcag attccgaaca gggcgggtac gatcgtctgg gtccacaggt cggatttggc
     3301 gatcggcagc cgcacggcaa cataggccag atagcccagc ccggcccaca aggtaaccgg
     3361 atgaagccgc cgcagggtcc tgaagtcatg cagtgccaga gccgcgacca ggggaacggc
     3421 gatcacggcc gaatccacga cgcttggccc gggccctgca aacagccggt aaatccgcga
     3481 ataagccggg gcgagcatgg tgatactggc catgatcata tagcgcttgt gatagtctgg
     3541 ccgcctggtc gcgcggaagc ccagcacaac cagcacggca aaccaggtgc tggtccccag
     3601 ctggatgaac agtcccgatt ggtcagacag cccgctgccg gtgttctttc cgtcaaacgc
     3661 ggtcaaagcc acgatccaca tcgcgctgag cacggcaagc gtggccagca ccatgcccag
     3721 ctgcccgccg atcgtgcgat gcccggccat attgcccttg cgcacttgca gcgcttgcca
     3781 caccagtagc gcgaaccagc ccgtgccgca gatcccgtgg atcaccagcg gcacggtcag
     3841 cgggcgggcc tgaaaaaccg gctggagaaa gaaggtacgg gcaaagccca gcgccagcac
     3901 cccggcaagg gccagactaa accagaacac aaaacccgaa cgccggcctt tgcgcgtgct
     3961 cgtggcaacc gtatccatat gcgactcccc catcgcaaat gatcgtcaga atggagctta
     4021 tcggcccgtt cccgccttgc caagggacca gcggtgccta tagggacccc atgcgcaccg
     4081 cccagatcgc ccgcaagacg catgaaaccg acattgccgt cgtagtgaac ctcgacggca
     4141 ccggggccta tgacgtctcc accgggatcg gctttctcga tcacatgatc gagcagttca
     4201 gccgccattc gctgatcgac atcacctgcc acatcaaggg cgacctgcac gtcgaccagc
     4261 accacaccac cgaggacagc gcgatcgcga tcgggcaggc catttcccag gccctgggcg
     4321 acaaggccgg gatcggccgc tacggcaccg cctacagccc gatggacgag gcgctggccc
     4381 gggtcgcgct ggatatctcg ggccggccct ggctggtctg gaacgcgcgc ttcacccagg
     4441 aacggctggg cgaaatggac accgagctgt tcgagcactg gttccaatcg atcgcccagg
     4501 cggtggggat cacgctgcac atcgagctgc tctatggcag caacaaccac cacatctgcg
     4561 aaggcatcta caagggtttt gcccgggcga tgcggcaggc ggtggagctg gacccgcgca
     4621 aggccggggc gatcccttct accaaaggcc agctcggtgg ctgaggttct cgcgctggtc
     4681 gattacggcg cgggcaatct tcactcggtg gcaaatgcgc tcaaggccgc cggcgcggag
     4741 aacgttaccg ttaccgccaa tcccgatgtg gtccgcgcgg cggaccggat cgtcctgccc
     4801 ggggtcggca gcttcaaggc ctgcgccgaa gggttgcgcg gcatccccca cctggtcgag
     4861 gccatggcgg agcgggtgct ggtgggcggc gcgccgttcc tgggcatctg cgtcggcatg
     4921 cagctgctgg ccaccaccgg cgtcgagcac ggtgaaaccg cgggcctcgg ctggatcggc
     4981 ggcgaagtgc ggctgatcga gcggaccgat ccggccatca agatccctca catgggctgg
     5041 aacgatgtcg aggtgacccg ccacgccgcg ctggtcgagg ccggcgaggc ctatttcctc
     5101 cattcctatc acttcgcgcc cgaggacggc caccacgttg cggcgatgac cgaccacggc
     5161 ggcgggctgg tcgcggccgt ggcgcgcgac aacctggtcg gcgtgcagtt ccatcccgaa
     5221 aagagccagg cctatgggct ggcgctgctg gcccgcttcc tggaatggaa accctgagat
     5281 gatcgtcttc cccgcaatcg acctcaaggc cggtcaggtc gtgcgcctgg ccgaaggcga
     5341 tatggaccgc gccaccgttt atggtgacaa tcccgcggcc caggccctgc tgtttgccga
     5401 ggccggatcg cagttcctcc acgtggtcga tctggacggt tccttcgccg gccgggccga
     5461 gaaccgcgcg gcggtggaag cgatcctcga ggcctttccc ggccacgtcc agctgggcgg
     5521 cgggatccgc acccgcgagg cggtggaagg ctggttcgat ctcggcgttt cccgcgtcgt
     5581 catgggttcg gccgcgctca aggaccccga gttcgtcaag gacatggcgc gcgagtttcc
     5641 cggcggaatc gtcgttgcgg tggacgcgaa ggacggcatg gtcgcgaccg agggctgggc
     5701 cgatgtgtcg gacgtcgaag tggtcgacct cgcccgccgg ttcgaggatg ccggggtggc
     5761 cagcctgctg ttcaccgaca tcggccgcga cgggctgctg aaaggcgtca atatcgacgc
     5821 cacggtcggc ctggcgcgcc gggtcgatat cccggtcatc gccagcggcg gggtgaaagg
     5881 cctcgacgac atccacgtcc tcgcgctcca cgcccacgaa gggatcgaag gcgtgatcac
     5941 gggccgcgcg ctttacgaag gccggctcga tctggcggcg gcaattgcga tggcgaaccg
     6001 ggcgtgaccg tccgtatccg cgtcatcccc tgtcttgatg tccgcgacgg gcgggtcgtg
     6061 aagggcgtca attttgtcga tctgaaggac gccggcgatc cggtcgagca ggcccgcgcc
     6121 tatgacgcgg ccggggcgga cgagctgtgc ttcctcgaca tttcggccag ccacgagggg
     6181 cgcggcaccc tgctcgacgt ggtgcggcgc accgccgaag tctgcttcat gccgctgacc
     6241 gtcgggggcg gggtccgctc ggtggaggat gcccgcgcgc tgctgctggc cggggccgac
     6301 aaggtggcgg tcaattccgc cgcggtcagc cggcccgaag tggtggccga cattgccggc
     6361 aagatgggca gccagtgcgt cgtcgcctcg gtcgatgccc ggcgcacgcc ccgcgggaac
     6421 tgggaaatct tcacccacgg cgggcgcaag gcgaccggga tcgacgcggt cgaacacgcg
     6481 gtgaagctgg ccgagcttgg cgcaggcgaa ttgctcgtca cctcgatgga cggcgacggg
     6541 acgcaagccg gttacgacct ggagctgacc cgcacgattg ctgacgcggt gtcggtgccg
     6601 gtgattgcca gcggcggggt cggtacgctc gatcacctgg tcgagggcgt gaccaagggc
     6661 cacgccagcg cggtgctcgc cgcctcgatc ttccacttcg gcacccacac cattgccgag
     6721 gcccacgccg ccttgcgcgc ggccggattg ccggcccggg gctgagcccg cagtcccggc
     6781 ttgggacggg gcggaatcgg cggttgccag ccggacgggc tgggcgcagg ggccgggcca
     6841 tggtcgcccg cattgcatcc cttgtccttc ttgccgccgc gctgtccact ccggcgctgg
     6901 cgcaggccgc cagcacggcg attcccgatc cctcgggcgt ggcgctgttc ggactgggcc
     6961 tggccggggt gatcctgggc cgccacctgt cgcgccgccg ccccggcggg gagtgaccct
     7021 cgcggcccgc ccgccccact tgacctgatc cgcgccgcgc cgcatggctt gggccatgga
     7081 cacgcttgcc cgcctcgaag ccacgatcgc cgcgcgccac ggcggcgatc ccgacgccag
     7141 ctatgtcgcc cggctgctcc acaaggggct gcccgaagtg gcccgcaagg tgggcgagga
     7201 agcggtcgaa accgccgttg cggccctggc gcacgagcgc gagcaagtgg tcggcgaagc
     7261 ggccgacctg atctttcacc ttatggtcct gctccgcgcg cgcgaaatcc cgctggccga
     7321 cgtgctggcc gagcttgacc gccgcgaggg caccagcgga atcgccgaaa aagcagcaag
     7381 gggcgcttaa atgccgatcg acgccaccca gccgtacgac gaccagaaca tcttcgcgaa
     7441 gatcctgcgc ggggagattc cgaaccggac ggtttacgag gacgaatggg cgctggcctt
     7501 tcacgatatc aacccgcagg cgccctgcca cgtgctggtg atccccaagg gggcctacgt
     7561 cagctgggat gacttttcgg ccaaggccgg ggccgaggag atcgccgggt ttgtccgcgc
     7621 ggtcggccat gtcgcgcgca cgctggggct ggtcgagccg ggctaccgcc tgctcgccaa
     7681 catcggcggc cacggccacc aggaggtgcc gcacctccac gtccacctgt tcggcgggcg
     7741 tccgctgggg ccaatgctgg cgcgctgaac gggccactcg gctcgcttca acgcagctga
     7801 agcacacaac cggcatggcc ccttgccctg cgactcgcgg acttgctaag ggtccgccgg
     7861 catgacgtca cagccttcgc ccccctccgg cctgattgaa ggcacgctgc accgctttgc
     7921 cgtgcgcgcc tatttcgagg acaccgacct gtcgggcgtg gtctatcacg ccaattacct
     7981 gcgctggttc gaacgcgccc ggtcggactt cgtccggctg ctggggatcg accagcgcgc
     8041 ggtgaacgaa agcggcgggg gcgcctttgc cgtggccgac ctgacgatcc gctatgccgc
     8101 cccggcccgg ctggacgatg ccgtgctgat cgagaccacc tgcaccgagc tgggcgcggc
     8161 ctcgtgccgg atgcaccaga aggccttccg cgaggacggc ctgctgctga ccgaagcgag
     8221 ctttcgcatc ggtttcgttt cccccgatgg ccgcccgcgc cggatgcccg atggctggcg
     8281 cgcggccttt gccccgatca tgcccgaagg aacccgatga ccgttcttgc cctgctgaat
     8341 gccgcggccg ctgcgcccaa gcacctcgat ccgctggcgc tgttcctcca ggccgacatt
     8401 gtcgtccagg cggtgatggc cgggctggtc ctggcctcgg tctggacctg gacgatcatc
     8461 gccggcttca gcctgcgcca cgcgcggctg cgcaaggatt gcgacagcta tgagcgcgat
     8521 ttctggaaag cgcccgacct cgatgccttc caggcagagc gcggcaaggc cgacatccct
     8581 tcggcccggg tcgccgcggc cgggatcggc gaatggcgcc gttccaccgg cggcaagaca
     8641 ctggaccgcg acggcacccg ccagcgcctg tcccaggcga tggacagcgc gatcgccatg
     8701 gaggcggaca agctggccga acggctcaac ttcctcgcca cggtcggctc ggtcgcgccc
     8761 ttcgtcgggc tgttcgggac
//
LOCUS       DASUDV010000244         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1487857,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000244 DASUDV010000000
VERSION     DASUDV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487857"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFV30_05305"
     CDS             complement(<1..629)
                     /locus_tag="VFV30_05305"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX4847538.1"
                     /translation="MPGDILDSQGRGDAHWSWPAFHPESHKFAGIVAAAAALSAYAAW
                     ETVAWPLAALVPCILAFFRDPRRVTPLDDRLIVAPADGLITLIQKVDPPRELVIDDGT
                     GAPGLGAEPLTRISIFMSVFDVHINRAPIGGTVRRVVYIPGKFMNADLDKASEENERQ
                     HILIDRGDGLMIGFTQIAGLIARRIVPFVKPGDMLAAGQRVGLIRFGSRV"
     gene            780..1844
                     /locus_tag="VFV30_05310"
     CDS             780..1844
                     /locus_tag="VFV30_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX4847539.1"
                     /translation="MTASTALGQKPALLSQLPQRLREDLPVYAISSLLLALCLAVLAA
                     KGIFFGLDGVFANTRFFLLSSAVVLVAGALWHLATVRPDRPLSHLSAHFLADPARREV
                     LLGAIPGLALLCLFMPFFSALKATIPLFATYDWDRTFIDLERSLLFGHDAWELLQPVL
                     GYPLITATLAVLYHLWILLCYPGTALFLFLNIDNRLRRRFLLSFVLTWSLVGGVLATL
                     LASYGPVFVGALAGIPDFDPQMAYLRAANEQVPVMTLVVQDLLLARFHEAESGLGSGI
                     SAMPSMHIAMCYLFYLAIRQVDRRLGWAAMAFGIAIWLGSVHLAYHYALDGVVSVIAV
                     AAIWKGCGWLIDRWDARLAA"
     gene            complement(1841..>2872)
                     /locus_tag="VFV30_05315"
     CDS             complement(1841..>2872)
                     /locus_tag="VFV30_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX4847540.1"
                     /translation="VDLFNARHAAEIEAGTIAALAPSSVEFIEKASGIKARHVMSKAS
                     ILDPEIMAPRWAERPDEELSVLAEMGVKAARDALAAAGRDPKDVDAVLCACSNLQRPY
                     PAIAIEIQNELGIEGFGFDMNVACSSATFGIQTAADFIRAGNARSVLVVSPEICTGHN
                     NMKNRDSHFIFGDVATAVLVEAKDMAPAVHWDIIGTRLKTVYSNNIRNNFGFLNCATP
                     ETIGTPDKLFVQEGRKVFKEVVPMVATMIQEHATDLGLDPAALRRLWLHQANQGMNRL
                     IAQRVLGHEASEDESPTVLDTYGNTSSAGSIIAFHRNSADLAAGDKGVICSFGAGYSA
                     GTVFVRKAA"
BASE COUNT          413 a          977 c          932 g          550 t
ORIGIN      
        1 acccggctgc cgaagcggat caggccgacg cgctggccgg cggcaagcat gtcgcccggc
       61 ttgacgaagg gcacgatccg ccgcgcgatc agcccggcga tctgggtgaa gccgatcatc
      121 agcccgtcac cccggtcgat caggatgtgc tggcgctcgt tttcttcgct cgccttgtcg
      181 agatcggcgt tcatgaactt gccgggaata tagaccaccc gccgcaccgt gccgccgatc
      241 ggggcgcggt tgatgtgcac gtcgaacacc gacatgaaga tcgagatccg ggtcagcggc
      301 tccgcaccca ggccgggcgc gccggtgccg tcgtcgatca ccagctcgcg cgggggatcg
      361 accttctgga tcagcgtgat cagcccgtcg gcgggcgcaa cgatcagccg atcgtccagc
      421 ggagtgacgc ggcgcggatc gcggaaaaag gccagaatgc agggcaccag cgcggccagc
      481 ggccaggcga cggtttccca cgcagcatag gcgctcagcg ctgcggcagc cgcaacgatg
      541 ccagcgaact tgtggctttc gggatggaag gcgggccagc tccaatgcgc atcgccgcgc
      601 ccctggctat cgaggatatc tccgggcatg gagggccatc taggcttttg ccgcgcgact
      661 aacaagcgcg cttgccattc gctcccccac gccgccctgc tatcccgtcc gcgaccggcc
      721 tgcctgcgcc acccggcatg cttaacactt tattgacagg ccatgcttat tccgggccca
      781 tgaccgcctc gaccgccctc gggcagaagc ctgccctgct gtcccagctg ccgcagcgtc
      841 tgcgcgaaga tctgccggtt tatgcgattt ccagcctgct gctggccctg tgcctggcgg
      901 tgctggccgc caagggcatc ttcttcgggc tcgacggggt ctttgccaac acccgcttct
      961 tcctgctgtc cagcgcggtg gtgctggtgg ccggggcgct gtggcacctc gccacggtcc
     1021 gaccggaccg cccgctcagc cacctatccg cccatttcct ggctgatccg gcccgccgcg
     1081 aggtgctgct gggggccatt ccgggccttg ccctgttgtg cctcttcatg ccgttctttt
     1141 cggcgctgaa ggccaccatt ccgctgttcg cgacctatga ctgggatcgc accttcatcg
     1201 atctggagcg cagcctgctg ttcgggcacg acgcctggga gctgctccag ccggtgctgg
     1261 gctatccgtt gatcaccgcg acgcttgcgg tcctctatca cctgtggatc ctgctctgct
     1321 atccgggcac cgcgctgttc ctgttcctga acatcgacaa ccggctgcgc cgccgctttc
     1381 tgctgagctt cgtgctgacc tggtcgctgg ttggcggggt gctggcgaca ctgctggcct
     1441 cctacggccc ggtcttcgtc ggcgcgctgg ccgggattcc cgatttcgat ccgcaaatgg
     1501 cctacctgcg cgccgccaac gagcaggtgc cggtgatgac cctggtggtg caggacctgc
     1561 tgctggcccg cttccacgag gccgaaagcg gccttggcag cgggatcagc gccatgcctt
     1621 cgatgcacat cgcgatgtgc tatctgttct atctggcgat ccgccaggtg gaccggcggc
     1681 tgggctgggc ggcgatggcc tttggcatcg cgatctggct cggctcggtc caccttgcct
     1741 accactacgc gctggacggc gtggtttcgg tgatcgccgt tgccgcgatc tggaagggct
     1801 gcggctggct gattgatcgg tgggacgcgc ggctggcggc ctaggctgcc ttgcggacga
     1861 agaccgtccc ggccgaataa cccgcgccaa acgagcagat cacacccttg tccccggccg
     1921 ccagatcggc gctgttgcgg tgaaaggcga tgatcgaccc cgcgctggag gtgttgccat
     1981 aggtgtcgag caccgtcggg ctttcgtcct cgctcgcttc atgacccagc acgcgctggg
     2041 cgatcaggcg gttcatgccc tggttggcct ggtgcagcca cagccggcgc agcgcggccg
     2101 gatcgagccc caggtcggtc gcgtgctcct ggatcatggt ggcgaccatc ggcaccactt
     2161 ccttgaacac cttgcgccct tcctggacga acagcttgtc cggcgtgccg atcgtctcgg
     2221 gcgtcgcgca gttgaggaag ccgaagttgt tgcggatgtt gttcgaatag accgtcttca
     2281 gccgggtgcc gatgatgtcc cagtgcaccg ccggggccat gtccttggcc tccaccagca
     2341 ccgcggtcgc cacgtcgcca aagatgaagt ggctgtcgcg gttcttcatg ttgttgtggc
     2401 cggtgcagat ttccgggctg accaccaaca ccgagcgggc gttgccggcg cgaatgaaat
     2461 cggccgcagt ctggatcccg aaggtcgcgc tggagcaggc gacattcatg tcaaagccga
     2521 agccctcgat ccccagctcg ttctggattt cgatcgcgat cgccggatag gggcgctgca
     2581 ggttggagca ggcgcacagc accgcatcga catccttggg atcgcgcccg gccgcggcca
     2641 gcgcgtcgcg cgccgccttg acgcccattt cggccagcac cgaaagctct tcgtccggcc
     2701 gctccgccca gcgcggggcc atgatctcgg gatcgaggat cgaggccttg ctcatcacgt
     2761 ggcgggcctt gatcccgctg gccttctcga tgaattccac cgaactgggg gccagcgcgg
     2821 cgatcgtccc ggcctcgatc tcggcggcgt ggcgcgcgtt gaacaggtca ac
//
LOCUS       DASUDV010000245         7233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_294942,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000245 DASUDV010000000
VERSION     DASUDV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294942"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFV30_05320"
     CDS             complement(<1..331)
                     /locus_tag="VFV30_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847541.1"
                     /translation="MFKTFGLTLALVASVASAPMAIASPASDQAAMLAQGLQSMVGPQ
                     EGKPLYVSKIVAEGEVLVIVVNGPAGWRQNMKAEDISAVFVAGLCSEASDVFNGGMKM
                     RLDTTEAG"
     gene            481..2262
                     /locus_tag="VFV30_05325"
     CDS             481..2262
                     /locus_tag="VFV30_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4847542.1"
                     /translation="MAAAFDFDPTSSLGDALVILGAAGIVIPLFARFRITPVIGFILA
                     GIVVGPFGLGALVEDRPWLDYLTISDPARLTPFAEFGIVLLLFSIGLELSFKRLWQMR
                     RTIFRIGPMELFGGGALIAMVLMVLGNSLPTALALGLALALSSTALVLKIADTRTPVG
                     RAALGTLLFEDIAIVPIIFLLGAMGRGDAAGDWGDFVRTLALGSLVIVALFGFGRFFL
                     PILFAQAARTKSPELFLSACLLVVIVAALVTGAVGLSPIFGALLAGLLIAETEYHGEV
                     EAMIEPFKGLALGIFLLTVGMSLDLGLIWERLGEIVVATALVLAAKALVTGMVLRLHG
                     FRTATATEAGILLASPSEMTLIVLSSALAVNLIRPDAAQFWQIVTALGLTITPLLAIL
                     GRWLGRRVDHASAALEDAEDGDHRRTLVIGGGRVGKLIADMLTTHAKPYLIIDADADQ
                     VRENRLNGYKTAFGDAARNDALARFGAAEASAVILTMDEPVAVQRMVRQLRKAYPDLP
                     IIARARDASHAAALYRAGASHAVPETLEASLQLSEAVLVDIGVPMGPVIASIHEKRDE
                     FRAQIMEEGELDAKPLLKSSSLRERGA"
     gene            complement(2267..2749)
                     /locus_tag="VFV30_05330"
     CDS             complement(2267..2749)
                     /locus_tag="VFV30_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847543.1"
                     /translation="MRIAEGLRKAIAAFGGVLFMAVSTFPMLGGAAKWEVQCRGRDFI
                     GRWDDCFNDQLPFFEMAAPFLALMLLWPFLRFANTLWSPDPQLRTKDWRLASENTKQV
                     LWPLLQWSGLALGIWCFHRAALYPIDPVTAPYQLTWVVFGLWALAGAVMAWPQRKAED
                     "
     gene            complement(2752..5397)
                     /gene="alaS"
                     /locus_tag="VFV30_05335"
     CDS             complement(2752..5397)
                     /gene="alaS"
                     /locus_tag="VFV30_05335"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX4847544.1"
                     /translation="MTSTNDIRRSFLDYFGSNGHEVVPSAPLVPYNDPTLMFTNAGMV
                     PFKNVFTGLETRAVPRATSSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KEQAITHAWTLLTKEWGLNPNRLTATVYHTDDEAAALWQKIAGLPEERIIRIPTKDNF
                     WAMGSDGPCGPCSEIFYDHGDHIFGGPPGSPDEDGDRFVEVWNLVFMQYEQAADEIVS
                     ELPKPSIDTGMGLERIAAVMQGVTDNYDTDTFKALIAASEGLTGVKAEGESKASHRVI
                     ADHLRSTSFLLADGVLPSNEGRGYVLRRIMRRAMRHAHLLGAKDPLMHRLVPALVQEM
                     GAAYPELGRAQPLIEETLQREEIQFRRTLANGLKLLDEATAGLGEGGELPGETAFKLY
                     DTYGFPYDLTEDALRSRGIGIDRAGYDAAMAQQKAAARAAWKGSGQAADEEVWFDIAE
                     RAGATEFTGYTATTGEAQVVALVKDGKEVDSAAAGDTVSVIVNQTPFYGESGGQMGDA
                     GTITGADGLVLAVSDTGKPLGRLHAHHAAVTAGTIKVGDTVKLDIDVARRDATRANHS
                     ATHLLHQALRNRLGEHVTQKGSLVAPDRLRFDFSHPTALSAEDIAVIEAEVNAQVRGN
                     EPVVTRLMTPEDAIEAGAMALFGEKYGDEVRVLSMGKTTERTYSTELCGGTHVRALGD
                     IGVFRIVSESAVSSGVRRIEALTGEGARQWLVGREDALKTAASLLKTTPDEVEARVAA
                     LLDERRKLERELAEAKKALALGGGGAKAEAADEAIGGVKFSGQVLDGLDPKELRGLLD
                     QEKARLGSGVAVVVAVNEGKASIAAAVTDDLTGSVSAVDLVRAGVEALGGKGGGGRPD
                     MAQGGGPDGDKAAEAIAAVKAVLAG"
     gene            complement(5453..6268)
                     /locus_tag="VFV30_05340"
     CDS             complement(5453..6268)
                     /locus_tag="VFV30_05340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4847545.1"
                     /translation="MTTGSDWREQVGQSWADNYRLTDRAFSGLTERLLARIGGCEGQR
                     VLDIGCGAGELSLAVARGNQGADVIGVDVSPALLEAARQRAGDHTNIRFVEADAGSWQ
                     AADFRPDKLISRHGVMFFDAPRQAFGNLRTNASPDAELVFSCFRSPRLNPWASELASL
                     LELQPSSDPHAPGPFAFADEGHVRGILGDAGWRDVALAEVDFAYVAGLGEDPVADALN
                     LFTRIGPAAPALRALQGDARERALGWMRDWLEQHRSGEIVALPAAAWIVTARG"
     gene            6429..>7233
                     /locus_tag="VFV30_05345"
     CDS             6429..>7233
                     /locus_tag="VFV30_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4847546.1"
                     /translation="MGNTQNGTNGGRAATRAVALVGPAGTGKTSLAEALLFASGTITR
                     QGSVEAGSTVGDASPEARARGSSTEINLLHFDYMGDRFALIDVPGGPGFSADGLAALQ
                     SADMALVVIDPVPERAALAEPMLRQLDTLGVPHAVFVNKIEHARAGAIRDLISELQPM
                     SREPLALRQIPIRTRDATGAEQVTGYVDLALERAYRYRPGKESERIAIPGELLEREQQ
                     ERAHLLETLADFDDALMEALLMDEVPDPALVLADLAADTRTNTIVPVLLG"
BASE COUNT         1115 a         2519 c         2396 g         1203 t
ORIGIN      
        1 tgccggcctc ggtcgtgtcg agccgcatct tcatgccgcc gttgaacacg tcgctggctt
       61 ccgagcagag cccggccacg aagaccgcgc tgatgtcttc ggccttcatg ttctggcgcc
      121 agccggccgg gccgttgacc acgatcacca gcacttcgcc ttcggccacg atcttcgaca
      181 cgtagagcgg cttgccctct tgcgggccaa ccatcgattg caggccctgg gccaacatcg
      241 ccgcctggtc gctcgccggg ctggcgatcg ccatcggagc gctggccacg ctggccacca
      301 gcgccagggt cagtccgaac gtcttgaaca tagggtattc cttccaacag gtttgaccac
      361 gccagctttt gcgaaagcgg ccgcgcagtc aagccaatcc cgccgctcgc accggaagac
      421 tgtgccagtc cggtgcagga cgaggtgaca aggccccgcg cggccgatat gccggaagcc
      481 atggccgcag cattcgattt cgaccccacc tcctcgctcg gcgatgcact ggtgatcctg
      541 ggcgcggcgg gaatcgtgat cccgctgttc gcccggttcc gcatcacccc ggtcatcggc
      601 ttcatcctgg ccgggatcgt ggtcggcccc tttggcctgg gcgcactggt ggaggaccgg
      661 ccgtggctgg actatctgac catcagcgat ccggcccggc tgacaccgtt cgctgagttc
      721 gggattgtcc tgctgctgtt ctcgatcggg ctggaactgt cgttcaagcg gctgtggcag
      781 atgcggcgga cgatcttccg gatcgggccg atggagctgt tcggtggcgg ggcgttgatt
      841 gccatggtgc tgatggtcct cggcaactcg ctgcccaccg cgctggcgct gggcctggcc
      901 ctggcgctgt cctctaccgc gctggtgctc aagatcgcgg acacccgcac cccggttggc
      961 cgggcggcgc tgggcaccct gctgttcgag gatatcgcga tcgtgccgat catcttcctg
     1021 ctgggcgcga tgggccgcgg cgatgccgcc ggcgattggg gcgatttcgt gcgcacgctg
     1081 gcgctgggca gcctggtgat cgtggccctg ttcgggtttg ggcgtttctt tctgccgatt
     1141 ctgttcgccc aggcggcccg caccaaaagc ccggagctgt tcctttccgc ctgtctgctg
     1201 gtggtgatcg tggccgcgct ggtcaccggg gcggtcggcc tctccccgat tttcggggcg
     1261 ctgctggccg gcctgctgat tgccgagacc gaataccacg gcgaagtcga ggccatgatc
     1321 gagccgttca agggcctggc cctggggatc ttcctgctga ccgtggggat gagcctggac
     1381 cttggcctga tctgggaacg gctgggcgag atcgtggtgg ccactgcgct ggtactggcc
     1441 gccaaggcgc tggtgacggg catggtcctg cggctgcacg gtttccgcac cgcgaccgcg
     1501 accgaagccg ggatcctgct ggccagccct tcggaaatga ccctgatcgt cctgtccagt
     1561 gcgctggcgg tcaacctgat ccggccggat gccgcgcagt tctggcagat cgtgaccgcg
     1621 ctggggctga ccatcacccc cctgctcgcg atcctgggac gctggctggg gcggcgggtg
     1681 gatcacgctt cggccgcgct ggaggatgcc gaggacggcg atcaccgccg caccctggtg
     1741 atcggcggcg gccgcgtcgg caaactgatc gccgacatgc tgaccaccca tgccaagccc
     1801 tacctgatca tcgatgccga tgccgatcag gtgcgcgaga accggctgaa cggctacaag
     1861 accgcctttg gcgatgccgc ccggaacgat gccctggccc gcttcggcgc cgcggaggcc
     1921 agcgcggtga tcctgaccat ggacgagccg gtggccgtgc agcgcatggt tcgccagctg
     1981 cgcaaggcct atcccgacct gccgatcatc gcccgcgccc gcgacgccag ccacgccgcc
     2041 gcgctgtacc gcgccggggc gagccatgcg gtgccggaaa cgctggaggc ctcgctgcag
     2101 ctgtccgaag ccgtgctggt cgatatcggc gtgccgatgg ggccggtgat cgccagcatc
     2161 cacgaaaagc gcgacgaatt ccgcgcgcag atcatggaag aaggcgaact ggacgcaaag
     2221 ccgctgctga agagctcgag cctgcgcgag cgcggagcct gagccgctag tcctcggcct
     2281 tgcgctgcgg ccaggccatg acagcaccag ccagagccca caggccgaac acgacccagg
     2341 tgagctggta gggagccgtt accggatcga tgggatagag cgcggcccgg tgaaagcacc
     2401 agatcccgag tgcgaggccg ctccattgca gcagcggcca caaaacctgc ttggtgtttt
     2461 cgctggcaag ccgccaatct ttcgttcgca attgcggatc aggcgaccag agcgtgttcg
     2521 cgaaacgcaa gaagggccac aatagcatca aggctaggaa aggcgccgcc atctcaaaga
     2581 agggcagctg gtcgttgaag caatcatccc acctgccgat gaaatcacgc ccgcggcatt
     2641 gcacctccca cttcgctgca ccaccaagca tcgggaacgt cgacacggcc atgaacagaa
     2701 caccgccgaa ggccgcgatc gctttgcgaa ggccttcggc tatgcgcatg gctagcctgc
     2761 cagaaccgct ttgaccgccg cgatggcctc ggccgccttg tcgccgtcgg gaccgccgcc
     2821 ctgggccatg tcgggccggc cgccgccgcc cttgccgccc agcgcctcga ctccggcgcg
     2881 gaccagatcg acagcgctga ccgagccggt cagatcgtcg gtcacggccg cggcgatgct
     2941 ggccttgcct tcgttgaccg cgacaaccac ggccacgccc gagccaagcc gggccttttc
     3001 ctgatcgagc aggccgcgca gctccttggg atcgagcccg tccagcactt ggccggagaa
     3061 cttgaccccg ccgatcgctt cgtcggccgc ttcggccttg gccccgccgc cgcccagcgc
     3121 cagcgccttc ttcgcttccg ccagttcgcg ttccagcttg cgccgctcgt cgagcagcgc
     3181 ggccacgcgg gcttccactt cgtcgggcgt ggtcttgagc aggctcgccg cggtcttcag
     3241 cgcgtcttcg cgcccgacca gccactgccg cgcaccttcg ccggtcagcg cctcgatccg
     3301 gcgcacgccg ctggagacgg cgctttccga cacgatccgg aacaccccga tatcgccgag
     3361 cgcgcggaca tgggtgcccc cgcacagttc ggtcgaatag gtccgctcgg tggtcttgcc
     3421 catcgacagg acgcgcacct cgtcgccgta cttttcgcca aacagggcca tggccccggc
     3481 ctcgatcgcg tcttcggggg tcatcagacg ggtcacgacc ggctcgttgc cgcggacctg
     3541 ggcgttcact tcagcctcga tcaccgcaat gtcctcagcg ctgagcgcgg tcgggtgcga
     3601 gaagtcgaac cgcaggcggt ccggcgcgac cagcgagccc ttctgcgtca cgtgctcgcc
     3661 caggcggttg cgcagcgcct ggtgcagcag gtgcgtggcc gagtggttgg cgcgggtggc
     3721 atcgcggcgc gcgacatcga tgtcgagctt gaccgtatcg ccaaccttga tcgtgcccgc
     3781 ggtcaccgcg gcgtgatgcg cgtggagccg gccgagcggc ttgccggtgt cgctgaccgc
     3841 cagcaccagg ccgtcggcgc cggtgatggt cccggcatcg cccatctggc cgccgctttc
     3901 gccatagaac ggcgtctggt tcacgatcac gctgaccgtg tcgccggccg cggcgctgtc
     3961 cacttccttg ccgtccttga ccagggcaac aacctgcgcc tcgccggtgg tggcggtgta
     4021 gccggtgaat tcggtcgccc cggcacgctc ggcgatgtcg aaccacacct cttcgtcggc
     4081 ggcctgcccg ctgcccttcc aggcggcgcg cgcggcggcc ttctgctggg ccatggcagc
     4141 gtcatagccg gcccggtcga tcccaatccc gcgggaccgc agcgcgtcct cggtcaggtc
     4201 ataggggaag ccgtaagtgt cgtagagctt gaaggcggtt tcacccggca gttccccgcc
     4261 ctcgcccaga ccggccgtcg cctcgtcgag cagcttgagc ccgttggcca gcgtacggcg
     4321 gaactggatc tcctcgcgct gcagggtttc ctcgatcagc ggctgggccc ggcccagctc
     4381 cggataggcc gcgcccattt cctggaccag cgccggcacc aggcggtgca tcagcggatc
     4441 cttcgcgccc agcaggtgcg catggcgcat cgcccggcgc atgatccggc gcagcacata
     4501 gccgcgccct tcgttgctgg gcagaacgcc atcggccagc aggaagctgg tcgaacgcag
     4561 atggtccgcg atcacccggt ggctggcctt gctctcgccc tcggccttca ccccggtcag
     4621 cccttcgctc gccgcgatca gcgccttgaa ggtgtcggta tcgtagttgt cggtcacgcc
     4681 ctgcataacc gcggcaatcc gctccagccc catgccggtg tcgatgctcg gcttgggcag
     4741 ctcgctgacg atctcgtcgg cggcttgctc atactgcatg aacaccaggt tccagacttc
     4801 gacaaagcgg tcgccgtctt cgtccgggct gcccggaggg ccgccgaaga tgtggtcacc
     4861 gtgatcgtag aagatctcgc tgcacgggcc gcaggggccg tccgaaccca tcgcccagaa
     4921 gttgtccttg gtcggaatgc ggatgatccg ctcttccggc aggccggcga tcttctgcca
     4981 cagcgccgcg gcttcgtcat cggtgtgata gacggtggcg gtcaggcggt tcggattgag
     5041 gccccattcc ttggtcagca gggtccaggc gtgggtgatc gcctgttcct tgaaatagtc
     5101 cccgaagctg aagttcccca gcatctcgaa aaaggtgtgg tgccgcgcgg tgtagcccac
     5161 gttgtcgaga tcgttgtgct tgcccccggc gcgcacgcat ttctggctgc tggtggcgcg
     5221 cggcacggcg cgggtttcca ggcccgtgaa cacgttcttg aacggcacca tcccggcgtt
     5281 cgtgaacatc agggtgggat cgttgtaggg caccagcggc gcagacggca ccacttcgtg
     5341 cccgttcgag ccgaagtaat cgaggaagga gcggcggatg tcgttcgtcg aggtcatgcc
     5401 gccgccgata aggcaggcgc ggcccagcgg caagcgtcaa aagccgggcg tttcagccgc
     5461 gcgcggtgac aatccaggcg gcggccggca aggcgacgat ctcgccgctg cggtgctgct
     5521 ccagccagtc gcgcatccag cccagcgccc gctcgcgcgc atcgccctgc agcgcgcgca
     5581 gcgccggggc ggccggaccg atccgggtga agagattgag cgcatccgcc accgggtctt
     5641 cgcccagccc ggcgacatag gcaaagtcca cttcggccag cgcgacatcc cgccaaccgg
     5701 catcgcccag gatgccgcgg acatggccct cgtcggcaaa ggcgaagggc ccgggggcgt
     5761 gcggatcgct ggatggctgc agttccagca ggctcgcaag ctcgctggcc caggggttca
     5821 gccgcgggct gcggaaacag gaaaagacca gctcggcatc ggggctggca ttggttcgca
     5881 ggttgccaaa ggcctggcgc ggggcatcga agaacatcac cccgtggcgc gagatcagct
     5941 tgtcgggccg gaaatcggcc gcctgccacg atccggcatc ggcctcgaca aagcggatat
     6001 tggtgtgatc cccggcgcgc tgccgcgctg cctcaagcaa ggccggggag acatcgacgc
     6061 cgatcacatc ggccccctgg ttgccgcgcg ccacggccag cgacagctcg cccgcgccgc
     6121 agccgatatc gagcacgcgc tgcccttcgc agccgccgat ccgggccagc agccgctcgg
     6181 tcaggcccga aaaggcccga tccgtcagcc ggtagttgtc ggcccagctc tggccaacct
     6241 gctctcgcca atccgatccc gtcgtcatgc ccgcaggcta ggccttgccc cggtgcggca
     6301 caaggcacaa atgctgcgca aaaatcactt gccagagcgg cgcgagaggc agagcatcgc
     6361 cccagtcctt gcgcgcccga gtgcaagtga gaatgcgccc ggcgcgcctc atgcaaaagg
     6421 aggcgacgat ggggaacacc cagaacggaa cgaacggcgg cagagcagcg acccgtgcgg
     6481 ttgcgctggt gggaccggcc ggcacgggaa agaccagcct ggcagaggcc ttgctgtttg
     6541 ccagcggcac catcacccgc cagggcagcg tcgaggccgg ctccacggtc ggcgatgcca
     6601 gcccggaagc ccgcgcccgc ggcagttcca ccgaaatcaa cctgcttcat tttgactaca
     6661 tgggtgaccg gttcgccctg atcgacgtgc ccggcggccc gggcttttcc gccgacggcc
     6721 tggccgcgct gcaatcggcc gacatggcgc tggtggtgat cgatccggtg ccggaacgcg
     6781 ccgccctggc cgagccgatg ctgcgccagc tcgatacgct gggcgtgccc cacgcggtgt
     6841 tcgtcaacaa gatcgagcat gcccgcgccg gggcgatccg cgatctgatc agtgaactgc
     6901 aaccgatgag ccgcgagccg ctggccctgc ggcagatccc gatccgcacc cgcgacgcga
     6961 ccggggccga gcaggtgacc ggctatgtcg atctcgcgct ggaacgggcc taccgctacc
     7021 gtcccggcaa ggagagcgag cgcatcgcca tccccggcga gctgctggag cgcgagcaac
     7081 aggaacgcgc ccacctgctc gaaaccctgg ccgatttcga cgatgcgctg atggaggcgc
     7141 tgctgatgga cgaagtgccc gatccggcgc tggtcctggc cgatctcgcc gccgataccc
     7201 ggaccaacac gatcgtgccc gtgctgctcg gct
//
LOCUS       DASUDV010000246         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_935749,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000246 DASUDV010000000
VERSION     DASUDV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935749"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..963)
                     /locus_tag="VFV30_05350"
     CDS             complement(91..963)
                     /locus_tag="VFV30_05350"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX4847547.1"
                     /translation="MTSIRTALLAGLALLSATPALAQEGLPPGAQSGPPSGPPPGMRD
                     DGKDRLTVGFGLGLAPDYEGSDDYELQPGGIFQGRVSGIEFQMRGLNLYTDLVPDKPG
                     SKTRLVLGPVVQVRLDRTQDLADPRVVQLGDRKTAVEVGLTAGIGKRGVLIPPASLNF
                     DVTFLHDINGAHRSYVITPSLALSSPVSQRSFARIGVSADYVGAGYARTYFDVAPGFT
                     LPAYSAGGAGFKSLGASVLYTHDLGGNPRKGWGLFGLVNGKRMLGRFADSPVVKDAGS
                     ASQVFAVAGLSYSF"
     gene            1053..2033
                     /locus_tag="VFV30_05355"
     CDS             1053..2033
                     /locus_tag="VFV30_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847548.1"
                     /translation="MTYRLFGALGSPYSQKLRALLRYRRIPHLWIDGAAAREALGQVR
                     APVIPVLQYPDGHYDNDSTPVIYDLEARHAERRVVPDDPGQAFIAHLLEDFADEWLTK
                     AMFGYRWLEEVDQVQMSRWLAFDNLKGGGLETSQKFAALFRDRQVGRMALVGCTAENF
                     PLIEASTRAVLSALEAHVVNRHCLFGTRPSLAEFGIFGQLSQLGTDPTPQAMMRADYP
                     YTYRWLAHIDDMSGVEGEWEPGFAPVVAELVRIAGDVYVPFLRANELAAEAGAETFRM
                     EAMGHPYEQGVFKYQLKCLRELRSRHAALPPASREEVDALLGPAWLALLG"
BASE COUNT          346 a          709 c          740 g          317 t
ORIGIN      
        1 cggcccagcc acccgatatg gttaccctgt gggcggctgg gccgggggag cgggccggtg
       61 ttgccggttt cagctctgct gaaacccaaa tcagaagctg taggacagcc cggccacggc
      121 gaagacctgg ctggcgctgc ccgcgtcctt caccaccggg ctgtcggcga accggcccag
      181 catccgcttg ccgttgacca gcccgaacag gccccagccc ttgcgcggat tgccgcccag
      241 gtcgtgggtg tagagcaccg atgcgcccag gctcttgaac ccggccccgc cggccgaata
      301 ggcgggcagg gtaaagccgg gggcgacatc gaaataggtc cgggcatagc cggccccgac
      361 atagtccgcg gaaaccccga tccgggcaaa gctgcgctgc gacacgggcg agctgagcgc
      421 gagcgagggg gtgatcacat agctgcggtg cgcgccgttg atatcgtgca ggaaagtgac
      481 gtcgaagttc agactggccg gtgggatcag caccccgcgc ttgccgatcc cggcggtcag
      541 ccccacttcg accgcggtct tgcggtcgcc cagctggacg acgcgcggat cggccaggtc
      601 ctgggtccgg tcgagccgca cctggaccac cgggcccaac accagccggg tcttgctgcc
      661 gggcttgtcg ggcaccagat cggtgtagag gttgagcccg cgcatctgga attcgatgcc
      721 cgaaacccgg ccctgaaaga tcccgccggg ctgcagctcg tagtcgtcgg agccttcgta
      781 gtccggcgcc aggcccaggc caaagcccac ggtcagccgg tccttgccat cgtcgcgcat
      841 gccgggaggc gggccggacg gcggacccga ttgggcaccc ggaggcagac cttcctgcgc
      901 caaagcggga gtggcgctca acagggcaag gcccgcgagc agggcggtgc ggatgctggt
      961 catggtgttg ctatactccc gatctcgccc cacaccggcg atataggcac gaagctggca
     1021 ggctcaacag tacgaaagga caggtacgcg gcatgacata ccggctcttc ggcgcgctcg
     1081 gctcgcccta ttcgcagaaa ctgcgcgccc tcttgcgcta ccgccggatc ccgcacctgt
     1141 ggatcgacgg ggccgccgcg cgcgaggcgc tgggccaggt ccgcgcgccg gtgatcccgg
     1201 tgctgcagta cccggacggg cattatgaca acgacagcac cccggtgatc tacgatctgg
     1261 aggcgcgcca tgccgagcgg cgggtggtgc cggacgaccc agggcaggcc ttcatcgccc
     1321 acctgctcga ggactttgct gacgaatggc tgaccaaggc gatgttcggc taccgctggc
     1381 tggaagaggt cgaccaggtg cagatgagcc gctggctggc ctttgacaac ctgaagggcg
     1441 gggggctgga aaccagccag aaattcgccg cgctgtttcg cgaccgccag gtcgggcgga
     1501 tggcgctggt aggctgcacc gcggaaaact tcccgctgat cgaggccagc acccgcgcgg
     1561 tcctctccgc gctggaagcc catgtggtga accgccactg cctgttcggg acgcggccca
     1621 gcctggccga attcggcatc ttcggccagc tatcccagct gggcaccgat cccacgccgc
     1681 aggcgatgat gcgcgccgac tatccctata cctaccgctg gctggcccac atcgacgaca
     1741 tgagcggggt cgagggcgaa tgggagccgg gctttgcgcc ggtggtggcc gagctggtcc
     1801 gcatcgcggg cgacgtttac gtgccgttcc tgcgcgccaa cgaactggcg gccgaggcgg
     1861 gggcggagac cttccggatg gaggcgatgg gccaccctta cgaacaaggc gtgttcaagt
     1921 accagctcaa atgcctgcgc gagctgcgca gccgccatgc cgccttgccg ccggcctcgc
     1981 gggaagaggt cgatgcgctg ctgggcccgg cctggctggc gctgctcggc tgattttcgc
     2041 agcggcccta cccatcgggg tagggcggcg gctccccggc cgtggcacgg cgagagggtc
     2101 cggacgcacc gg
//
LOCUS       DASUDV010000247         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1443584,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000247 DASUDV010000000
VERSION     DASUDV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443584"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VFV30_05360"
     CDS             complement(<1..1153)
                     /locus_tag="VFV30_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX4847549.1"
                     /translation="MTDLFRQEVFDHRADRLYGEVIINQPVAQRYYVAALGLICALAV
                     AWVCLGTYARTETAPGILVPDRPAPKILPLAPGNVRELKVQEGSEVRAGDILAVIEID
                     RQTESGDGVAAASLSTIDARAALGEEQVRLSSSRLRGEQARLSAVAATADSQIADLSG
                     QIELQREIVKSNKTLFEQAGTLVEKGFVSKIEYERRRQAWLGSLQQLSGLEQQRTNQL
                     GQAAQARAQLGSAQAQSLSEQSEIRAGLQSLEQQRAGIESQKSYVVRAPVTGRVTTVL
                     TNIGSTANPGSPLMTIVPEGARMKAEIYAPSRAIGFVHAGQETKLLYDAFPYQRFGSF
                     KGRVESISRVLIDPRETPIPLKLEEPVYKVTVLLERQDVQAFRQVYPLQP"
     gene            1439..>3087
                     /locus_tag="VFV30_05365"
     CDS             1439..>3087
                     /locus_tag="VFV30_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4847550.1"
                     /translation="MTGRRIGAVWAQLTGLGARLWRDRTARYVIVIILVLGALGSNLP
                     FEPYDWLQKSVTARLNAKPYTGDAIIIEIDNQTFEGLGRPALDDRDLARLVRNLDAAK
                     ASRIVLGRIRQRSASQRSSPELLEALDAASPKVVLFTELVPKKSSAFSWATFRPANGQ
                     AYFHDPGIDPPTARRVQLASEISSTTPFGAPWALPPTVRLGAQDYPSIAQIMVGQPQP
                     IFDKYDVALQVDPGSIPTLNAIDVISGRFDPAAIRGKQALIYSSSDVTRDAIVTPFGR
                     LPSAAPITVMAAETLRNGPPLMLGWIPAFVVAVLGIVGWMTLRSPLLRRGAFALAFVC
                     VVASPLVLERLLIFQATSNAVFLMILVALGRQWSKFRATLALARSAAETKSWFLAQAS
                     HDLRQPIHAIGMLAARLGQTDLNPAQADLARKIDRSIEAANRMLQSLLDLATIESGSL
                     SPQPAPISVNALLAELDEQAGPLAERAGVELRFVPCEATIMADRTLTLAMLQNIVANS
                     IKYATGKRVLVGARRTGKTIALCVHDVGQGISDSDMRHVRKA"
BASE COUNT          505 a         1034 c          983 g          565 t
ORIGIN      
        1 cgggctggag gggatagacc tggcggaagg cctgcacgtc ttgccgttca agcagaaccg
       61 tgaccttgta gaccggctct tcgagcttga gcgggatggg ggtctcgcgc ggatcgatca
      121 ggacccgcga gatcgattca acccggccct tgaagctgcc gaagcgctgg taggggaagg
      181 catcgtaaag cagcttggtt tcctggccgg catggacgaa tccgatcgcc cggctggggg
      241 catagatttc ggccttcatc cgcgcgcctt cgggaacgat ggtcatcaac ggactgccgg
      301 gattggcggt gctgccgatg ttggtcagga ccgtggtgac ccggccggtc accggggcgc
      361 ggaccacata ggatttctgg ctctcgatcc cggcgcgctg ctgctcgagc gattgcagcc
      421 cggcccggat ctcgctctgt tcgctcaggc tctgggcctg ggcgctgcca agctgggcgc
      481 gggcctgtgc ggcctggcca agctggttgg tgcgctgctg ctcgagcccg gacaattgct
      541 gcaggctgcc aagccaggcc tgacgccggc gttcgtattc gatcttgctg acaaagccct
      601 tctcgacgag cgttccggcc tgttcgaaca gggtcttgtt ggacttgacg atttcgcgct
      661 gcagttcgat ctggcccgac agatcggcaa tctggctgtc ggcggtcgcc gccacggccg
      721 aaagccttgc ctgctcaccc cggagccgcg aggaggacag gcgaacctgc tcttcgccaa
      781 gcgcggcccg ggcatcaatg gtcgagagcg atgcggctgc aacgccatcg ccggattcgg
      841 tctggcgatc gatttcgatc accgccagaa tatcgccggc ccttacctcg ctgccttcct
      901 ggaccttgag ttcgcggaca ttgcctgggg ccagcggcag gatcttgggt gccggccggt
      961 cgggtaccag aatgcccggc gcggtctcgg ttcgggcata ggtgccaaga cagacccagg
     1021 ccacggccag ggcgcagatc agacccagcg ccgcgacgta atagcgctgg gcgaccggct
     1081 ggttgatgat aacctcgcca taaagccggt cggcacgatg gtcgaagacc tcctggcgga
     1141 acagatcggt caccgcgcgc cttccccctt gccagcttgc ccgccgcgcc cggcgatgcc
     1201 agtcgcaatg ccgcctgggc accgggaccg gttacgagct aggggcaacg gcagatacat
     1261 ggcaagaccc gatcactctg ttaagtgcct gggcagactg ctttgcttgc cggtgccatg
     1321 caataggaca gctgcactaa agcgggcttc cccaccccga tacgcttgac cctggcgcaa
     1381 caagccggaa caatcgcggg caaggctgcg cagccggaat tcgatttggg ggcacaggat
     1441 gacaggacgc cgcatcgggg cggtttgggc acagctgacc ggccttggcg cccgcctctg
     1501 gcgggatcgc acggcgcgtt atgtgatcgt gatcatcctg gtcctgggcg cgctcggcag
     1561 taacctgccg ttcgaaccct acgactggct gcaaaagtcg gtgaccgccc ggctcaacgc
     1621 caagccctac acgggtgatg cgatcatcat cgagatcgac aaccagacat tcgaagggct
     1681 tggccgccct gcccttgacg acagggatct cgccaggctg gtccgcaatc ttgacgcggc
     1741 caaggccagt cggatcgtgc tgggccggat tcgccagcgt tcagcctcgc agcgctccag
     1801 ccccgaactg ctcgaagcgc tggatgcggc cagcccgaag gttgtcctgt tcaccgagct
     1861 tgtccccaag aagtcgagcg ccttttcctg ggctactttc cggccggcca atggccaggc
     1921 ctatttccac gatccgggca tcgatccgcc gaccgcgcgg cgggtccagc tggcaagcga
     1981 aatcagttcc accacgcctt tcggcgcgcc atgggccctg cccccgacgg tgcggctcgg
     2041 cgctcaggac tatccctcga tcgcccagat catggttggc cagccgcaac cgatcttcga
     2101 taagtacgac gtcgccctgc aggtcgatcc gggttcgatc cccacgctca atgcgatcga
     2161 cgtgatctcg ggccggttcg atccggccgc aatccgcggc aagcaggcgc tgatctattc
     2221 ctcgtccgac gtcacccgcg atgccatcgt gacgccattc ggccgcctgc cttcggccgc
     2281 gccgatcacc gtcatggctg ccgagaccct gcgcaacggc ccgcccctga tgctgggctg
     2341 gattccagcg ttcgtggttg cggtcctggg tatcgtgggc tggatgaccc tgcgttcgcc
     2401 gctgctgcgg cgcggcgctt tcgcgctggc gttcgtctgc gtggtcgcat cgccgctggt
     2461 ccttgaacgg ctgttgatct tccaggcgac ctcgaacgcg gtgttcctga tgatcctggt
     2521 cgcgctcggc cgccaatgga gcaagttcag ggcaaccctg gccctggccc gctcggccgc
     2581 cgaaaccaag tcctggttcc tggcccaggc cagccacgat ctgcgccagc cgatccatgc
     2641 gatcggcatg ctcgccgcgc gccttgggca aaccgatctc aatccggccc aggccgacct
     2701 tgcccgcaag atcgaccgct cgatcgaagc ggccaatcgc atgctgcaat cactgctcga
     2761 tctggcgacg atcgaaagcg gctcgctatc gccgcagcct gccccgatat ccgtcaacgc
     2821 cttgctcgcg gagctcgacg agcaggccgg gccgctcgcc gaacgcgccg gtgtggagct
     2881 gcgcttcgtc ccctgcgagg ccacgatcat ggccgatcgt acgctcacgc ttgcgatgtt
     2941 gcagaacatc gtcgccaatt cgatcaagta cgcaaccggc aagcgcgttc tggtcggcgc
     3001 ccggcggact ggcaagacca ttgcgctttg cgtccacgac gtcggccagg gcatatcaga
     3061 ctccgatatg cggcatgtgc gcaaggc
//
LOCUS       DASUDV010000248         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1112481,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000248 DASUDV010000000
VERSION     DASUDV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112481"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1117)
                     /locus_tag="VFV30_05370"
     CDS             complement(62..1117)
                     /locus_tag="VFV30_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4847551.1"
                     /translation="MKLWLYRIGTALALAFLVLTFVNASWLADSPRGYIKLIAHRGAY
                     QLFDHAGVERDTCTASRIEPPVHDFLENTLPGMSQAVRSGAQMIELDIALTRDGKLAV
                     FHDWTLDCRTNGTGHVRDATMAELKALDAGYGYSADGGKTFPFRGKGVGLIPELSEVL
                     QAFPDKPILFNFKSRDHEEAEVLARWLKAAGRDPVKLGDGFYGATESGPVSRIRRLFP
                     GAWVFSKESVKACTSAYAWQGWLAMTPAVCKNGTVAIPLDRQWAFAGWPNRMIERMNA
                     VGARIILVGPQNGPGGLGLDLPEQVREIPAGFTGYVWVEDIWSVGPAVRPGFNKRNPR
                     EEEELARALEARRKARE"
     gene            complement(1114..2037)
                     /locus_tag="VFV30_05375"
     CDS             complement(1114..2037)
                     /locus_tag="VFV30_05375"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX4847552.1"
                     /translation="MPRLDARDAMPERLRGAIIALGNFDGFHRGHQAVAGAAIARARA
                     EGRPAIVATFDPHPVRFFAPDAPPFRLTTLDQRQELFEAAGADAMLVFGFDAALAATS
                     AEDFVADLLARRLAAAGVVTGEDFTFGKGRKGNVSLLAELAAAAGMSAEAVRAVAQGG
                     EVVSSSRIREALKAGNCEEATRLLTRPFAIRGLVQHGDKLGRTIGFPTANLPLGNYLR
                     PRYGVYAVSARLADGRVLKGAANLGIRPQFDPPKELLEPHFFDFAEDLYGREIEVALH
                     HFLRGEEKYASLEALTAQIARDCDTARELLG"
     gene            complement(2040..>2326)
                     /locus_tag="VFV30_05380"
     CDS             complement(2040..>2326)
                     /locus_tag="VFV30_05380"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX4847553.1"
                     /translation="GIGYWDGAVCDNSPRSIARSIKYVRDLVGIQHVALGSDYDGATT
                     VRFDTSGLAQLTQALLDEGFTPDEIRAVMGGNALRVIRAGIVPMGAAAPQ"
BASE COUNT          395 a          787 c          775 g          369 t
ORIGIN      
        1 gcgcttcagt catagcgggc tgccgttaga gcagaagcgg caaacgcgct agtccccgcc
       61 cttattcgcg cgccttgcgc cgcgcttcga gtgcgcgggc gagttcctct tcctcgcgcg
      121 gattgcgctt gttgaagccg ggacgcaccg ccgggccgac cgaccagatg tcctccaccc
      181 agacatagcc ggtaaagccg gcgggaatct cgcggacctg ttcgggcagg tcgagcccca
      241 ggccgcctgg cccattctgc gggccgacca ggatgatccg cgcgcccacc gcgttcatcc
      301 gctcgatcat ccggttgggc cagccggcga aggcccattg ccggtcgagc gggatcgcca
      361 ccgtgccgtt cttgcagacc gccggagtca tcgccagcca gccttgccag gcataggcgc
      421 tggtgcaggc cttgacgctc tccttgctga acacccaggc gccgggaaac agccgccgga
      481 tccgcgaaac cggcccgctt tcggtggcac cgtaaaaacc atcgcccagc ttcaccggat
      541 cgcgcccggc ggctttcagc cagcgggcca gcacttcggc ctcttcgtgg tccctgctct
      601 tgaaattgaa caggatcggc ttgtcgggga aggcctggag gacttcggac agttcgggga
      661 tcagccccac gcccttgccg cgaaagggaa aggtcttgcc gccatcggcg ctgtagccat
      721 agccggcatc aagtgccttc agctcggcca tggtcgcatc gcggacatgg ccggtgccgt
      781 tggtgcggca atcgagcgtc cagtcatgga acacggccag cttgccatcc ctggtgagcg
      841 cgatgtcgag ctcgatcatc tgcgcgcccg aacgcaccgc ctggctcatg ccgggcagtg
      901 tgttttcaag gaaatcgtgg accggcggct cgatccggct ggcggtgcag gtatcgcgtt
      961 ccaccccggc gtggtcgaac aactggtagg cgccgcgatg cgcgatcagc ttgatatagc
     1021 cgcgcggcga atcggccagc cagctggcat tgacgaaggt gagcaccaga aaggcaaggg
     1081 ccagggccgt tccgatccgg taaagccaca gcttcatccc agcagttccc tcgccgtgtc
     1141 gcagtcgcgt gcgatctgcg cggtcaatgc ctcgagcgag gcgtattttt cctcgccgcg
     1201 caggaaatgg tgcagcgcaa cctcgatctc ccggccgtag aggtcttcgg caaagtcgaa
     1261 gaagtgcggc tcgagcagtt ccttgggcgg atcgaattgc gggcggatcc caagattggc
     1321 cgcgcctttc agcacccggc catcggccag ccgcgcggag accgcataga ccccgtagcg
     1381 cgggcgcaga tagttgccga gcgggaggtt ggcggtggga aagccgatcg tgcggcccag
     1441 cttgtcgcca tgctgaacca ggccgcggat cgcgaagggg cgggtgagaa gccgggtggc
     1501 ctcttcgcag ttcccggcct tcagcgcctc gcggatccgg ctggaggaga caacctcgcc
     1561 gccttgcgcc accgcgcgca ccgcctcggc gctcatgccc gccgccgcgg cgagttcggc
     1621 gagcagcgaa acattgccct tgcggccctt gccgaaggtg aaatcctcgc cggttacaac
     1681 ccctgcggca gcaagccggc gggcgagcag gtcggccacg aaatcttcgg cgctggtcgc
     1741 ggccagcgcc gcatcgaaac cgaagaccag catcgcatcg gccccagcgg cttcgaacag
     1801 ttcctggcgc tgatccagcg tggtcagccg gaacggcggg gcatcgggcg caaagaagcg
     1861 caccggatgc ggatcaaagg tggccacgat cgccgggcgc ccctcggccc gggcgcgggc
     1921 aatcgcggcg cccgcaacgg cctggtggcc acggtggaac ccgtcgaaat tgcccagcgc
     1981 gatgatcgcg ccgcgcaagc gttcgggcat ggcgtcgcgc gcatcgagac gaggtatggt
     2041 cattgcgggg cagcggcccc catcggcacg atcccggcgc ggatcacgcg cagcgcattg
     2101 ccgcccatca ccgcgcggat ctcgtcagga gtgaaacctt cgtcgagcaa cgcctgggtc
     2161 agctgggcga ggcccgaggt gtcgaagcgc acggtggtcg ccccgtcata gtcgctgccc
     2221 agggcaacgt gctggattcc caccagatcg cggacatatt tgatcgaccg ggcgatcgag
     2281 cgcggcgaat tgtcgcagac cgcgccgtcc cagtacccga tcccga
//
LOCUS       DASUDV010000249         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_692598,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000249 DASUDV010000000
VERSION     DASUDV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692598"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1445)
                     /locus_tag="VFV30_05385"
     CDS             complement(219..1445)
                     /locus_tag="VFV30_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEX4847554.1"
                     /translation="MSQLLSLAGIALILAIAYILSSDRKAIRLRVVGAAFALQAGFAA
                     LVLYVPWGNQLLQGIAAGVSALLGYAGKGTEFLFGALASDKLGQNFAIQALPVIIFFA
                     ALISVLYYLGIMQLVIKWVGGALEKITGISKVESLSAASNIFVGQSESPLVIRPYLAA
                     LTPSQLFCLMTVGMAGVAGTILAAYASMGIRIDYLVAAAFMSAPGGILMAKIIMPDPK
                     GETIVEPDEIKIPDEEERPANVIMAAAQGAQTGVKLAVMVGAMVLAFVALVALANGIL
                     GGIGGLFGQPDLSFQMILGYVFAPVFVLLGVPDWAEAMQAGGLFGTKVVLNEFVAFIE
                     LGAASALSEKTVAIVTFALCGFANFSSIAIQMAVTGGLAPNQRPMIAKLGIKALAAGS
                     LSNLMSAALAGLFLSL"
     gene            1590..>2119
                     /locus_tag="VFV30_05390"
     CDS             1590..>2119
                     /locus_tag="VFV30_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX4847555.1"
                     /translation="MQNPAPAIPRSLFVLAILYGGMVPLGGFLGAKQVTLGPLAVEAG
                     IFPFLTLVALSSAIAELHGSDTANRLVRYGFVPLIVAIFWTFLVVRLPTDPGMYEPAK
                     EAFPIIMAQGARMMAAGIVAYGVSVTLNIWLFDRLRNAVGKFAALRGFIAAALSQIVD
                     TILFIGISFYGVRPIM"
BASE COUNT          360 a          702 c          699 g          358 t
ORIGIN      
        1 gcgttgatgt cggccagccg caccttctct ccggcgatcc agaaggtgtc gccatcgacc
       61 acgcagttga tccggggggc atcgccgcac agggtgaagg cggcagcgga aagggcagca
      121 agaaacgggt ccatgcacgc ggcatggccc ctgtttccta acaggcggta tccgggaaac
      181 tacgcggttt cgctccgctc cggaggctta gcgccggctt agaggctgag gaacaggccg
      241 gccagcgccg cgctcatcag gttggacagg ctgcccgcgg ccagggcctt gatccccagc
      301 ttggcgatca tcggacgctg gttgggcgcc aggcccccgg tcaccgccat ctggatcgcg
      361 atcgagctga agttggcaaa gccgcacagc gcgaaagtga cgatcgccac ggtcttttcc
      421 gaaagcgcgc tggccgcgcc cagttcgatg aaggcgacga attcgttgag caccactttg
      481 gtgccgaaaa gcccgccggc ttgcatcgct tcggcccagt ccggcacgcc cagcaggacg
      541 aagaccgggg cgaagacata gccgaggatc atctggaacg agaggtcggg ctggccgaac
      601 aggccgccga tcccgcccag gatgccgttg gccagcgcca ccagcgccac gaaggccagc
      661 accatcgcgc cgaccatcac cgccagcttg accccggttt gtgcgccctg ggcggcggcc
      721 atgatcacgt tggcggggcg ctcttcctcg tccgggatct tgatctcgtc gggctcgaca
      781 atggtttcgc ccttgggatc gggcatgatg atcttggcca tcaggatccc gcccggggcc
      841 gacatgaagg cggcggcgac caggtagtcg atccggatcc ccatgctggc ataggccgcc
      901 aggatcgtgc ccgccacgcc ggccatgccc acggtcatca ggcagaacag ctggctgggg
      961 gtcagcgcgg caaggtaggg ccggatcacc agcgggcttt ccgactggcc gacaaagatg
     1021 ttcgaggcgg ccgacaggct ttccaccttg ctgatcccgg tgatcttttc cagcgcgccg
     1081 ccgacccatt tgatcaccag ctgcatgatc cccaggtaat agaggaccga aatcagcgcg
     1141 gcgaagaaga tgatcaccgg cagggcctgg atcgcgaaat tctggcccag cttgtccgag
     1201 gcgagcgccc cgaacaggaa ttcggtgccc ttgccggcat agcccagcag cgccgaaacc
     1261 ccggcggcga tcccctgcag caactggttg ccccacggca cgtacagcac cagcgccgca
     1321 aagccggcct gaagcgcaaa ggccgcgccg accacgcgca ggcggatcgc cttgcggtcg
     1381 gacgagagga tgtaggcaat cgccagaatc agggcgatgc cggccaggct gagcagttgc
     1441 gacatggtgg tgcggtcccc cggggaatag gtgtcgggcc cggtggtaac ggctttgcgc
     1501 ttccggtcaa ttcccgcgcg ggcgcaagct gtgggtggag cgcaccgggg gcatttactt
     1561 gccgcgccgg tgcgttaagc cttggcgcca tgcagaaccc ggcccccgcg atcccgcgct
     1621 cgctcttcgt ccttgccatc ctctatggcg gcatggtgcc gctcggcggc ttccttggcg
     1681 ccaagcaggt cacgctcggg ccgctggcgg tagaggccgg gatctttccc ttcctgaccc
     1741 tggtggcgct gtcctccgcg atcgccgaac tgcacgggtc ggacacggcc aaccggctgg
     1801 tccgctatgg cttcgtgccg ctgatcgtgg cgattttctg gaccttcctc gtcgtccgcc
     1861 tgccgaccga tccgggcatg tacgaaccgg ccaaggaagc cttcccgatc atcatggccc
     1921 agggcgcgcg gatgatggcg gccgggatcg tcgcctatgg cgtgtcggtc acactcaaca
     1981 tctggctgtt tgaccggctg cgcaacgcgg tgggcaaatt tgccgcgctg cgcgggttca
     2041 tcgccgcggc gctttcgcag atcgtcgaca cgatcctgtt catcggcatc agcttctacg
     2101 gggtgcgccc gatcatgga
//
LOCUS       DASUDV010000250         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_295027,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000250 DASUDV010000000
VERSION     DASUDV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295027"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /gene="crtI"
                     /locus_tag="VFV30_05395"
     CDS             complement(<1..1164)
                     /gene="crtI"
                     /locus_tag="VFV30_05395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX4847556.1"
                     /translation="MAALKMDHCSRRAKHYNPRAMAPRKKKAPVQPSGPRACVVGAGL
                     GGLALAIRLQAGGVQTVLVEARDAPGGLIRTWQQGGFTFDAGPAALADPGPLRELWSL
                     TGEDMADALALHEVTPAWRCNWPDGTVLDLPADPAQLARLAPDDLPGMEDFATWNRTA
                     RADGWQRLAEAAPSGLRALAGALPALARNQAWRSAAGLVASMVKEPHLREALAMPALL
                     AGANPYATSALTLIGQQGPGLGPAWWPEGGMGRLIAALADRFTRLGGELRLHDPVVRF
                     HAIGNRVHEVECASGWRGTFDTVASNADVVHTYRDLLAETPRGPDKAAKLTGQRFSPS
                     AFTVHFALEGTWPGIPHRSVLFAPRFKGLIEDVFVHGVLPQDMLIWLAHPSVTD"
     gene            1163..1693
                     /locus_tag="VFV30_05400"
     CDS             1163..1693
                     /locus_tag="VFV30_05400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847557.1"
                     /translation="MLIRPATPADAPALAAIYADHVLGGTASYEIVPPDAAEMAARLA
                     RIQAAGWPWLVAADADGTVTGYAYASQFRDRAAYGFTCENSIYIRADRRGEGLGTALM
                     GALIEAAKACGFRQMIAVIGGGEPASVALHAKAGFAHAGRLTAIGRKHGRWLDTVYMQ
                     LPLGPGDSLPPDAEPG"
     gene            complement(1694..>2145)
                     /locus_tag="VFV30_05405"
     CDS             complement(1694..>2145)
                     /locus_tag="VFV30_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX4847558.1"
                     /translation="RAIAIRRDRVAVAFASVLTPSEAKDLIAFYSSPVGRKMMKAVSN
                     NLVSDSQIDTAFEKDDPKINKAARDQDHQVTVNSTMADLLPNLTREEIRQLEAFGKTP
                     GFRKMDAVGQVLESIPEPSFEEISTPAERDAFRKAIVGALVPAVSGS"
BASE COUNT          311 a          750 c          767 g          317 t
ORIGIN      
        1 atcggtcacg ctggggtggg ccagccagat cagcatgtcc tgcggcagca cgccgtggac
       61 gaagacgtcc tcgatcagac ccttgaagcg cggggcgaac agcacgctgc ggtggggaat
      121 gcccggccag gtgccttcca gcgcgaaatg gacggtgaag gccgagggcg aaaagcgctg
      181 cccggtcagc tttgcggcct tgtccggccc gcgcggcgtt tcggccaaca gatcgcggta
      241 ggtgtggacc acgtcggcgt tgctggccac cgtatcgaag gtgccgcgcc agccgctggc
      301 gcattccact tcgtgcacgc ggttgccgat cgcatggaac cgcaccaccg gatcgtgcag
      361 ccgcagctct ccgcccagcc gggtgaaccg atcggccagg gcggcgatca gccggcccat
      421 cccgccttcg ggccaccagg ccggccccag ccccgggccc tgctgcccga tcagggtcag
      481 ggcgctggtg gcataggggt tggcgcccgc cagcagggcc ggcatggcca gcgcctcgcg
      541 caggtgcggt tccttgacca tcgaggcgac cagcccggcg gcgctgcgcc aggcctggtt
      601 gcgggccagc gcgggcaggg ccccggccag ggcacgcagc ccgctgggcg cggcttcggc
      661 cagccgttgc cagccatcgg cccgcgcggt ccggttccag gtggcgaaat cctccatccc
      721 cggcaagtcg tccggcgcca ggcgggccag ctgggcggga tcggccggca ggtccagcac
      781 ggtcccgtcg ggccagttgc agcgccaggc tggggtcacc tcgtgcaggg ccagcgcatc
      841 ggccatgtcc tcaccggtca gcgaccacag ttcgcgcaag gggcccggat cggccagggc
      901 cgcggggccg gcgtcaaagg tgaagccacc ctgctgccag gtacggatca gcccgcccgg
      961 cgcatcgcgc gcctccacca gcacggtctg caccccgccg gcctgcaacc ggatcgccag
     1021 cgccagaccg cccagccccg cgccgaccac gcaggcgcgc gggccgctgg gctggacggg
     1081 ggccttcttc ttgcgcggtg ccatggcccg ggggttgtag tgcttggcgc ggcggctgca
     1141 atggtccatc ttcaaggccg ccatgctgat ccgccccgcc acccctgccg atgccccggc
     1201 gctcgccgcg atctatgccg accatgtcct gggcggcacc gccagttacg agatcgtccc
     1261 gcccgatgcg gccgagatgg cggcccggct ggcgcggatc caggcggctg gctggccctg
     1321 gctggttgcg gccgacgcgg acgggacggt caccggctat gcctatgcca gccagttccg
     1381 cgaccgggcg gcctacggct tcacctgcga gaacagcatc tacatccgcg cggaccgccg
     1441 cggcgaaggg ctgggcacgg cgctgatggg ggcgctgatc gaagcggcga aagcctgcgg
     1501 gttccggcag atgatcgcgg tgatcggcgg aggcgagccg gcctcggtcg cgctccacgc
     1561 gaaagccggc tttgcccatg ccgggcggct gacggcgatc ggccgcaagc acggccgctg
     1621 gctcgatacg gtctacatgc agttgccgct tggccccgga gacagcctgc ccccggacgc
     1681 cgagccgggc tgatcaggat cccgagacgg ccggcaccag cgcgccgaca atcgccttgc
     1741 ggaacgcgtc gcgctctgcc ggagtagaga tttcctcaaa cgagggttcc ggaatggact
     1801 cgagaacctg gcccacggcg tccatcttgc ggaagcctgg ggtcttgccg aaggcttcca
     1861 gttggcggat ttcctcgcgg gtcaggttcg gtagcaggtc ggccatggtg gagttgaccg
     1921 tgacctggtg atcctgatcg cgggcggcct tgttgatctt ggggtcatcc ttttcgaacg
     1981 cggtgtcgat ctgggaatcg gacaccaggt tgttggacac ggctttcatc atcttgcggc
     2041 ccaccggaga gctgtagaag gcaatcaggt ccttcgcttc ggacggcgtc aggaccgaag
     2101 caaaggctac ggccacccgg tcccgccgga tcgcgatggc ccggc
//
LOCUS       DASUDV010000251         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1841646,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000251 DASUDV010000000
VERSION     DASUDV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841646"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /locus_tag="VFV30_05410"
     CDS             complement(<1..2006)
                     /locus_tag="VFV30_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4847559.1"
                     /translation="MSGYRMEQGGRLDRAQRVTFTFDGQRYEGLAGDTLASALLANGV
                     SLFGRSFKYHRPRGLLGAGVEEPNALISVDRGEGRFTPNLRATQIAIYEGLNARSQNR
                     WPSLKADLGAINDRLGRFFPAGFYNKTFMWPRSFWEKLYEPAIRRMAGLGDAPTAIDP
                     DHYAATHVHCELLVVGGGPAGIAAALEAAGTGQRVYLIDEQDELGGGALADPAQWAWL
                     EDSRKALAAADNITVLTRTTAFGYFHDNFIAAVERITEHLAEPGEGPREKLWKIRAGE
                     VVLAQGAIERPLVFEGNDVPGVMLASAARTWALRYGVAVGRRVVVMAAHDSGWNDAFA
                     LAGAGVGVAAIIDVRGRVDPALTDQAAALGIPVELGSSVIGVSGRHAVSAVTIARNDG
                     QGQRKLACDAVLMAGGWTPSIHLWSHSKGSIAWSEDWGAFLPDQPNEKVRCVGACGGA
                     WSFGSGINLGQLPTPKDPATIKAFVDFQNDVTAKDIALAVREGYKSVEHIKRYTTNGM
                     ATDQGKTSNLNALQLASGFLDRPIEKIGLTTFRPPYTPQTFGALAGHAKDALFQPTRK
                     TPIDGWAEAHGAVYEPVALWRRARYFPKPGEDMLAAVNRECLAVRNAVGIFDASTLGK
                     IEVVGPDAAEFLNRRYTNPWKALEPGRCRYGLLLKEDGFITDDGV"
     gene            complement(2003..2275)
                     /locus_tag="VFV30_05415"
     CDS             complement(2003..2275)
                     /locus_tag="VFV30_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX4847560.1"
                     /translation="MLLIHCPYCDEHLPEVEFTYAGEAHIARPEDPSQLSDEEWERYL
                     FIRANPKGRHNERWRHSFGCGRFFNAVRDTVTDKFELTYKAGEPRR"
     gene            complement(2277..>2735)
                     /locus_tag="VFV30_05420"
     CDS             complement(2277..>2735)
                     /locus_tag="VFV30_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4847561.1"
                     /translation="EPVKPVFPCVVMSNTVHAYMSQSDKGELVIGAGTDQYVSYSQRG
                     ALHILEHNIAAICEIFPIFRRMRMLRTWGGIVDVTPDRSPILGKTPVPGLYVNCGWGT
                     GGFKATPGAGDLLAYTMAKDEPHRINAPFNLDRFRGGRLIDEAAAAAVAH"
BASE COUNT          427 a          947 c          911 g          450 t
ORIGIN      
        1 acgccatcgt cggtgatgaa gccgtcttcc ttcagcagca ggccatagcg gcagcggccg
       61 ggctccagcg ccttccacgg gttggtgtac ctccggttca ggaactcggc ggcgtccggg
      121 ccaaccactt cgatcttgcc aagggtcgag gcgtcgaaga tccccaccgc attgcgcaca
      181 gccaggcatt cgcggttgac cgccgccagc atgtcctcgc ccggcttggg gaagtaacgc
      241 gcacggcgcc acagcgcgac cggttcatag accgcgccgt gggcctcggc ccagccatcg
      301 atcggggtct tgcgggtcgg ctggaacagc gcgtccttgg catggccggc cagcgcccca
      361 aaagtctgcg gggtataggg cgggcggaag gtggtcagcc cgatcttctc gatcggccgg
      421 tccagaaagc ccgaggccag ttgcagggcg ttgaggttcg aggtcttgcc ctggtcggtc
      481 gccatgccat tcgtggtata gcgcttgatg tgctcgaccg acttgtagcc ttcgcgcacg
      541 gccagggcga tgtccttggc ggtcacgtcg ttctggaaat cgacgaaggc cttgatcgtg
      601 gccggatcct tgggcgtggg cagctggccc aggttgatcc cgctgccaaa gctccacgcg
      661 ccaccgcaag cgccgacgca gcggaccttt tcgttcggct gatcgggcag gaacgcgccc
      721 cagtcctcgg accaggcgat gctgcccttg ctgtgcgacc acaggtgaat gctgggggtc
      781 cagcccccgg ccatcagcac cgcatcgcag gccagcttgc gctgcccctg tccgtcgttg
      841 cgggcgatgg tgactgcgct caccgcatgg cggcccgaaa cgccgatcac gctgctgccc
      901 agttccaccg gaatgccgag tgcggcggcc tgatcggtca gcgccggatc gaccctgccg
      961 cgcacgtcga tgatcgcggc cacgccaacg cctgccccgg ccagggcaaa ggcatcgttc
     1021 cagccgctgt cgtgggcggc catcaccacc acccgccggc caaccgccac gccatagcgc
     1081 agcgcccagg tccgcgcagc agaggccagc atgacgcccg gcacgtcgtt gccttcgaac
     1141 accaacgggc gttcaatcgc gccctgggcc agaacgacct ctccggcgcg gatcttccag
     1201 agcttctcgc gcgggccctc gccgggttcg gccagatgct cggtgatccg ctccaccgcg
     1261 gcaatgaagt tgtcgtggaa atagccgaac gcggtggtcc gggtcagcac ggtgatattg
     1321 tccgccgcgg ccagggcctt gcggctgtcc tccagccagg cccactgcgc cggatcggcc
     1381 agcgcgccgc cgcccagttc gtcctgctcg tcgatcaggt agacccgctg gccggtgccg
     1441 gcggcttcca gcgcggccgc gatgccggcc ggcccgccgc ccacgaccag cagttcgcag
     1501 tgcacatggg tggcggcata gtgatcggga tcgatcgcgg tcggcgcatc gcccaggccg
     1561 gccatgcgcc ggatcgccgg ctcatagagc ttttcccaga acgagcgcgg ccacatgaag
     1621 gtcttgttgt agaagcccgc cgggaagaac cggcccagcc ggtcgttgat cgcgccaagg
     1681 tccgccttca gcgaaggcca gcggttctgg ctgcgggcgt tcagcccctc atagatcgcg
     1741 atctgggtgg cgcgcaggtt gggcgtgaag cgcccctccc cccggtcgac cgagatcagg
     1801 gcgttgggct cttccacccc ggcacccagc agcccgcgcg ggcggtggta tttgaacgag
     1861 cggccgaaca ggctgacccc gttcgccagc agcgcggagg ccaaggtatc cccggccagc
     1921 ccttcatagc gctgcccgtc gaaagtgaag gtgacccgct gcgcgcggtc cagccgcccg
     1981 ccctgctcca tccggtaccc gctcatcgcc gcggctcccc ggccttgtag gtcagctcga
     2041 acttgtcggt caccgtgtcg cgcaccgcgt tgaagaagcg tccgcagcca aagctgtggc
     2101 gccagcgctc gttgtgccgc cctttcgggt tggcccggat gaacaggtag cgctcccact
     2161 cctcgtcaga gagctgcgag gggtcttcgg ggcgggcaat gtgcgcctcg ccggcatagg
     2221 tgaattccac ctccggcagg tgctcgtcgc aataggggca atggatcagc agcatatcag
     2281 tgcgccaccg cagcggccgc ggcctcgtcg atcaggcgcc cgccgcggaa ccggtcaagg
     2341 ttgaacgggg cgttgatccg gtgcggctcg tccttggcca tggtgtaggc gaggagatcg
     2401 ccggcgccgg gcgtggcctt gaacccgccg gtgccccagc cgcaattgac gtagaggccg
     2461 gggaccggcg tcttgcccag gatcggcgag cgatcggggg tgacgtcaac aatcccgccc
     2521 caggtgcgca gcatccgcat ccgccggaaa atcgggaaaa tctcgcagat tgcggcgata
     2581 ttgtgctcca gaatgtgaag cgcgccgcgc tgcgaatagc tgacgtactg gtccgtcccg
     2641 gcgccgatca ccagctcgcc cttgtcggac tggctcatat aggcgtggac cgtgttcgac
     2701 atgaccacgc aggggaacac cggcttgacc ggttc
//
LOCUS       DASUDV010000252        12384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2018435,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000252 DASUDV010000000
VERSION     DASUDV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018435"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..740
                     /gene="infC"
                     /locus_tag="VFV30_05425"
     CDS             219..740
                     /gene="infC"
                     /locus_tag="VFV30_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX4847562.1"
                     /translation="MMTPPVKSGPRYNQMIVSDKVRVIDENGENLGVMYTREAIEQAA
                     DVGLDLVEISPNADPPVAKFLDVGKFRYEAQKKANAARKNQKTQEIKEIKMRPNIDDH
                     DYDTKMRNVNRFIEDGDKVKITLRFRGRELSHQQLGMNLLKRVQEDVAEIAKIESYPR
                     MEGRQMLMVLSPK"
     gene            769..2172
                     /locus_tag="VFV30_05430"
     CDS             769..2172
                     /locus_tag="VFV30_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX4847563.1"
                     /translation="MTAKRIGFWLGLAGLVATLVLPAPAGMPGPAWIAAGLVWIMALW
                     WMTEALPLSATALLPFLVLPLTGIADATKTAGAYYSPIMFLFIGGAFLALAIERTGLH
                     RRLALFIMGLAGATPGGLLLGVMLATALLSGMISNTSTALIMMPMALAMLAAGGIREG
                     ETNGIAGALPMGIAFAASIGGLGTMIGTPTNAIAAGLLDKTLGVKIGFLEWSLYGMPI
                     VLLGVPLAALIIARVQKLGTEQFDPRAARTAIATADKWTAPERRLMPVVALALAAWIA
                     QPWLEGLFPKGGLTDGTIAAVVGLVLFFLPDGTGRPMLTWPEANRAPWDVVLMFGGGL
                     AMAMGMTESGLAAWLGKMLLPLSAVPLPLVALILVAFVIVVTEFASNIATASGIMPVV
                     AALVAALGADPMLLALPAAMASTWGFMLPAGTGPNAIAWSTGHLRMGRMIRAGFLLDL
                     AGVFLIVGVVWTVAAIT"
     gene            complement(2174..2644)
                     /locus_tag="VFV30_05435"
     CDS             complement(2174..2644)
                     /locus_tag="VFV30_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847564.1"
                     /translation="MTRLRIALLVLLGLFSAGAAMGAQAKERCLDGLKPALLAGGFSG
                     STDCGRDALSIARIGTVRAHGREYTVFDYRYQLASKCRDCAIHGGQRILFFRAGRYVG
                     QYKPDSADLAIVGGRLVLSPTSGMPGARPVTLRFTRRGPPMQVWFDGSNLEFFR"
     gene            2848..4140
                     /locus_tag="VFV30_05440"
     CDS             2848..4140
                     /locus_tag="VFV30_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX4847565.1"
                     /translation="MADTPARKPKASITRIGNRDLSPATLMMGHGYDPVLSEGSLKAP
                     IFLTSTFAFPSAADGKRFFEGITGKVPGGTDGLVYSRFNGPNQQILEGRLAVWDGAEE
                     ALCFSSGMTAITVLLLALCNAGDVIVHSGPLYAASEGFVAKHMSRFGVTYRDFPAGAT
                     RAEIDAVLEEAKAQAARQGGKVAMIYLESPANPTNALVDVEAVRDARDAALGADCPIA
                     IDNTFLGPLWARPIDQGADLVVYSLTKYVGGHSDLVAGSVSGAKRWITPVRMLRNTMG
                     GIADPNTAWMLMRSLETVELRMTRAGENAAKVCAWLKDHPKVAGLGYLGMIDDPRQQD
                     IFDRHCSGAGSTFSLLLKGGEAECFRFLDSLKIAKLAVSLGGTETLASHPASMTHLSV
                     PDERKAALGITDNLIRISIGIEDADDLIADFEQALAAV"
     gene            4150..5310
                     /locus_tag="VFV30_05445"
     CDS             4150..5310
                     /locus_tag="VFV30_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX4847566.1"
                     /translation="MPLRIAAASVLLAGMSAPAPDRPGSEAATLDAATAALCGRQIAV
                     LGEAEHGDARTEAFKIALIERLVSRCGYTAVVFEASFYEFVQLDREQRAGRPLTPGQI
                     ATAVGGLWKWNREFQPLLPYLASQANSGRIRLGGFDFQQGGREQDFSNTGVVAELTAE
                     LPPERRQTCRALFRDLVFKGSSPERRQAVAPCLTEMGALPPSADRTVRRERRELLANL
                     AAFTAADPSDSTGYTAQRDRAMFANFERWKARWPAGSKIIVWTATAHAARAPHGHKGF
                     QGVQPFGAYLAGRYGQAAFALGFAAQGGTTRGRPQPRVIAPPAPGTLEAWAGPIAPGG
                     ALFLDRAALARAGERPAGLFNHGPVTATWSRLLDGVVVFDAERAPADIRKGG"
     gene            5355..5843
                     /locus_tag="VFV30_05450"
     CDS             5355..5843
                     /locus_tag="VFV30_05450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4847567.1"
                     /translation="MSHEPHDKLQSAADVPGTSRLVEMTAGEWAGWYHWEPVDDFEEH
                     TGPFYCKPDGDALVCGFRPEAKNRNGGGNVHGGALMTFADYALFMTASGMDGSVHGVT
                     LTMNCEFVGPAETGRLLTARGEIVRQGFSIVFVRGIIDDEGRPVLTFSGTIKRLTPRE
                     KR"
     gene            5935..6081
                     /locus_tag="VFV30_05455"
     CDS             5935..6081
                     /locus_tag="VFV30_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847568.1"
                     /translation="MTKTSQPTPRWTKPELEKLGTLKDVAGGTFAGNDSNGQGQGSNR
                     NPVS"
     gene            6154..8022
                     /locus_tag="VFV30_05460"
     CDS             6154..8022
                     /locus_tag="VFV30_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX4847569.1"
                     /translation="MASSLGLPAPETLRFHRDGAAGYALTPGLFGAPAGRPGRIPVPA
                     ILPSGDRVLFTGWFDNRAAIAARLGLPDADPATVYGHAVARWGDEAECHVIGSYAAVV
                     DRPGRGEVRLARSPFSAPPLHFHHSARMAAAASVPRALFALGLPAELDEERLAMHLHA
                     AVTDRPGGWYKGIEALQLGAVATLTRAGVRRARPYDSAANPPVRLKRDADYVAAAEAL
                     LDEAIDATLPGFRQPATLLTGGLDSTLVARHLLDRMPADQRLPSFTWTCEPGHEGRDS
                     AFHFADERPRVEAFAAMHPRIEPHFLDNAGLGFDDGMQDLFLLAGVAPAAIGLIYPYH
                     GALAAARQRGCDLVIGAGGGNTTFSAEGRSAYTEYLLSGRWRQLWRALAARPGDTRPV
                     WRRLVSLSLLRALPEPLWRAASRWRGAIPGNPHRTFGALNPAWPGRAALERQAAADDP
                     GLTRPFYRTRAEEVRAIAAQTDADGFDLMQGLQQKYRIAFRDVTLYRPLVEFCRGLPV
                     DQLMRDGESRYLARRMGQGRLPEAIRTETRYGLQHGDWHWRIGQRRAALLAELRQLRE
                     DPAVGRMVDVGRLIALLEAFPDSPGTDPDLAYQYQIALPSGIAAARLIRYVRGTNA"
     gene            8015..9697
                     /locus_tag="VFV30_05465"
     CDS             8015..9697
                     /locus_tag="VFV30_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4847570.1"
                     /translation="MPDAPPLPPRLIGPEHHRPIAALVALMTLAAATEGLGIILLVPL
                     LGALGQGDSGSIGAMLRTIGLPLRLDLLLALFVGLVALRAVVVHLRTLAGMRFETAVV
                     DALRHRAWAALLHCEWRALLGMSRSNTASLLINRVDRAGTYVNQGILAASTLVTLGGV
                     ALAALAIAPLLTAGAVLAGALVLLAFHGMRRRAAALGDALGKAYAGVHGRLDEGLGAL
                     RAIKGLGREDKALTDLVGEFAGLRETSLAYLRDVGRGQIVLQAGGAAVLAVLVWLALE
                     RWHSDAAVVLPMVALFARALPLIGTLQQAGLNAAHARPAVDEALALIARAEAAREADA
                     GPGAPPALDRAIRLEGVTVRFEGEPRPALDTVTASIPARGITAITGPSGAGKSTLADL
                     IGGLLSPDDGTVRIDDTVLAGPARRAWRSRVAYVQQDPVLLAASLRDNLRWAAPEADD
                     AALAAALRAASAGFALDLPLGLDTRLGDGGRALSGGERQRLMLARALLRDPALLILDE
                     ATSALDPGNEAEIAAALEGLRHRMAVVVIAHRGTLADRADQVIRLENGRIAA"
     gene            complement(9694..10428)
                     /locus_tag="VFV30_05470"
     CDS             complement(9694..10428)
                     /locus_tag="VFV30_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4847571.1"
                     /translation="MDALIIAAGFGSRLRDISDSKPMTPIAGIPLLELGVRQAKAAGV
                     ERVVVVTGHEADRLEAALPALSERAGIPVVAQRVEDWSKPNGWSVLAGAAVIPGNYLL
                     MMSDHIFSGSILTRLAQQHGPDRGVTLAIDTRCDHPLIDPDDATWVKRDEAGFIRAIG
                     KTIDPYDAVDCGAFLCTPELAEAIRTAIAQGKAGSLSEGMQILADAGRAATMDIGEAW
                     WLDVDDPRAYELAEAQAPRELPEVYG"
     gene            complement(10496..11596)
                     /locus_tag="VFV30_05475"
     CDS             complement(10496..11596)
                     /locus_tag="VFV30_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX4847572.1"
                     /translation="MKPIKVAMIGVGNCASSLVQGVAYYRQNNSSQGLIHDKIGGYGA
                     GDVDFVLGIDVDARKVGKDIAEAIFAQPNNTKVFMPTVPATGAKVIMGKVSDGVAAHM
                     TTVGEKGFIVSDEAEADHARIVQAIKDSGAEVLINFLPVGSQEATEFYMECALEAGVA
                     VVNCMPVFIASKPEWEARFREKNIPIVGDDIKAQVGATIVHRVLSSLFAARGVTVERT
                     YQLNTGGNTDFMNMLDRQRLGSKKESKTEAVQAMLAQRLEDENIHVGPSDYVPWQKDN
                     KLCFLRLEGLQWGNVPMELELRLSVEDSPNSAACVMDAIRCAKVALERGEGGALIGPS
                     AYFCKHPPQQFNDDLAAQMVDEYVAEAPLAAE"
     gene            11851..>12384
                     /locus_tag="VFV30_05480"
     CDS             11851..>12384
                     /locus_tag="VFV30_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4847573.1"
                     /translation="MQRPQRPRELQDPLNHYLYHPLAWQFARLLAPTPITPNMVSVLG
                     AGFVVAAAFAYTQLTWPVSAALGMILHMTWHVVDGADGDLARMTGRSSPIGEMVDGLC
                     DYLSHAVLYIALAILLAGGAGVFSGWAAWVLVIGAGISHAVQANHVEVQRRQYQWWVY
                     GTAWIRNSHGGSDSATSR"
BASE COUNT         1835 a         4302 c         4291 g         1956 t
ORIGIN      
        1 ccggcccgct cccccggccc aaccacccca agggtaccat gtcacgggtg gttgggccgg
       61 gggagcgggc cggtgatgcc atggacaagg cggatgcctt gtccgcaagg ggtcccgaca
      121 aaacaagaca ccttccccaa accggcaatt cgttttaagg acccgcgcga gctttcgctg
      181 caacaggaga gaatactata gcacccccac cccggcgcat gatgacgccc cccgtgaaga
      241 gcgggccgcg ctacaaccag atgattgtct cggacaaggt tcgcgtcatc gacgagaacg
      301 gcgagaatct gggcgtcatg tacacccgcg aggcgatcga acaggctgcc gatgtcggcc
      361 tcgatctggt cgagatctct cccaacgccg atccgccggt ggccaagttc cttgatgtcg
      421 gcaagttccg ctacgaggcc cagaaaaagg ccaacgccgc gcgcaagaac cagaagacgc
      481 aggagatcaa ggagatcaag atgcgtccga acatcgacga tcacgattac gacaccaaga
      541 tgcgcaacgt gaaccggttc atcgaggacg gcgacaaggt caagatcacc ctgcgcttcc
      601 gcgggcgcga actgtcgcac cagcagctgg gcatgaacct gctcaagcgg gttcaggaag
      661 acgtggccga gatcgccaag atcgagagct atccgcgcat ggaaggccgc cagatgctga
      721 tggtgctttc gcccaaataa ggccggaccg gcagaacagg ggcccgggat gaccgccaag
      781 cgaatcggtt tctggctggg cctggcgggc ctggtcgcga ccctggtcct gcccgccccg
      841 gccggcatgc ccggccccgc ctggatcgcg gccggcctgg tctggatcat ggcgctgtgg
      901 tggatgaccg aggcgctgcc actgtcggcc accgcgctgc tgccgttcct ggtcctgccg
      961 ctgaccggaa tcgccgatgc caccaagacc gccggggcct attactcgcc gatcatgttc
     1021 ctgttcatcg gcggggcctt tctggccctg gcgatcgagc ggaccggcct gcaccggcgg
     1081 ctggcgctgt tcatcatggg cctggccggg gcgacccccg gcgggctgct gctaggggtg
     1141 atgctggcca cggcgctgct ttcggggatg atttccaaca cctcgaccgc gctgatcatg
     1201 atgccgatgg ccctggccat gctggccgcc ggcgggatcc gcgaggggga gaccaacggg
     1261 atcgccggcg cgctgccgat gggcatcgcc tttgccgcct cgatcggcgg gctggggacg
     1321 atgatcggca cgccgaccaa cgccatcgcc gccgggctgc tcgacaagac cctgggggtc
     1381 aagatcggct ttctcgaatg gtcgctctac ggaatgccga tcgtgctgct gggggtgccg
     1441 ctggcggcgc tgatcattgc ccgggtccag aagctgggca ccgaacagtt cgatccgcgc
     1501 gcggcccgca ccgccatcgc cacggccgac aagtggaccg cacccgaacg ccgcttgatg
     1561 ccggtcgtcg ccctggccct ggcggcctgg atcgcccagc cctggctgga gggcctgttc
     1621 cccaagggcg ggctgaccga cggcacgatt gccgccgtgg tcgggctggt gctgttcttc
     1681 ctgcccgacg gcaccggccg cccgatgctg acctggcccg aagcgaaccg cgcgccgtgg
     1741 gacgtggtgc tgatgttcgg cggcgggctg gccatggcca tgggcatgac cgaaagcggg
     1801 ctggcggcct ggctcggcaa gatgctgctg ccactgtcgg ccgtgccgct gccgctggtc
     1861 gcgctgatcc tggtggcctt cgtgatcgtg gtgaccgaat ttgccagcaa catcgccacc
     1921 gccagcggga tcatgccggt ggtggcggcg ctggtcgccg cgctgggcgc cgatccgatg
     1981 ctgctggccc tgcccgccgc gatggccagc acctggggct tcatgctgcc ggccggaacc
     2041 ggccccaacg ccatcgcctg gagcaccggc cacttgcgga tggggcggat gatccgggcc
     2101 gggttcctgc tcgacctggc cggcgtgttc ctgatcgtcg gcgtggtctg gaccgtcgcc
     2161 gcgatcacct gatctagcgg aagaattcca gattggagcc gtcgaaccag acctgcatgg
     2221 gcgggccgcg ccgcgtgaac cggagtgtca ccggccgggc ccccggcatc ccggacgtgg
     2281 gcgacaggac gagacggccg ccaacgattg ccagatcggc gctgtccggc ttgtactggc
     2341 ccacatagcg gcccgcgcgg aagaaaagga tccgctggcc gccgtgaatc gcgcagtccc
     2401 ggcacttgga ggccagttga tagcgataat cgaagaccgt gtattcgcgc ccgtgcgcgc
     2461 ggacagtgcc aatccgcgcg atgctcagcg cgtcgcgtcc gcagtcggtc gatccgctga
     2521 aaccgcccgc cagcaaggcc ggcttcaggc cgtccaggca gcgctccttg gcctgcgcgc
     2581 ccatggcagc gccggcggag aaaaggccca gaaggacaag gagggcaatg cgcagcctgg
     2641 tcatgaggcg aggcctacca tggcccgcga gtcgctcaaa ggcgcccgta tccggttgca
     2701 gttgaaatca aatcgccggc tgggtatgca cgccccatcc gggtccggga ggagatgcgc
     2761 gcctgcggag gttaggcgcg ccagtgcccc tgcggcccct ttggctcccg ccttcctggg
     2821 agcgtgatcg aaggaaggat gccgcacatg gcagataccc ccgcgcgcaa acccaaggcc
     2881 agcattaccc ggatcggcaa ccgcgatctt tcgcccgcca cgctgatgat gggccacggc
     2941 tatgatcccg tgctctccga aggcagcctc aaggcgccga tcttcctgac cagcaccttt
     3001 gcctttccca gcgccgccga tggcaagcgg ttcttcgaag ggatcaccgg caaggtgccc
     3061 ggcggcaccg atggcctggt ctattcgcgc ttcaacgggc ccaaccagca gatcctggaa
     3121 ggccgcctgg cggtgtggga cggggccgag gaggcgctgt gcttttccag cgggatgacc
     3181 gcgatcaccg tgctgctgct ggcgctgtgc aacgctggcg acgtgatcgt ccattccggc
     3241 ccgctctatg ccgccagcga aggctttgtc gccaagcaca tgagccggtt cggcgtgacc
     3301 tatcgcgatt tccccgccgg ggcgacccgc gccgagatcg acgcggtgct ggaagaggcc
     3361 aaggcccagg ccgcgcgcca gggcggcaag gtggcgatga tctatctgga aagccccgcg
     3421 aacccgacca acgccctggt cgatgtcgag gcggtgcgcg acgcccgcga tgccgcactg
     3481 ggagccgact gcccgatcgc gatcgacaac accttcctgg gcccgctgtg ggcccgcccg
     3541 atcgaccagg gcgcggacct ggtggtctat tcgctgacca aatatgtcgg cggccattcc
     3601 gatctggtcg ccggctcggt ctcgggcgcg aagcgctgga tcacgccggt ccggatgctg
     3661 cgcaacacca tgggcgggat cgccgatccc aacaccgcct ggatgctgat gcgcagcctg
     3721 gaaacggtcg agctgcggat gacccgcgcc ggtgagaacg ccgcaaaggt ctgcgcctgg
     3781 ctgaaggatc accccaaggt tgcgggcctc ggctatctcg gcatgatcga cgatccgcgc
     3841 cagcaggaca tctttgaccg ccactgctcg ggcgcgggca gcaccttttc gctgctgctg
     3901 aagggcggcg aggcggaatg cttccgcttc ctcgattcgc tcaagatcgc caagctggcg
     3961 gtcagcctgg gcgggaccga gacactggcc agccaccccg ccagcatgac ccacctgtcc
     4021 gtccccgacg agcgcaaggc agcgctgggg atcaccgaca acctgatccg gatctcgatc
     4081 gggatcgagg acgcggacga cctgatcgcc gatttcgaac aggccctggc cgcggtatag
     4141 cctgcggcca tgcccctgcg aatcgccgcc gcgtcggtcc ttctggccgg gatgtccgcc
     4201 ccggccccgg accggcccgg atccgaagcc gccaccctgg acgcggcgac tgcggcgctg
     4261 tgcgggcggc aaatcgcggt cctgggtgag gccgaacacg gcgacgcgcg gaccgaggcc
     4321 ttcaagatcg cgctgatcga gcggctggtt tcgcgctgcg gctataccgc ggtcgtgttc
     4381 gaggcgagct tctacgaatt cgtccagttg gaccgtgaac agcgggcggg ccggcccctg
     4441 actcccgggc agatcgccac cgcggtgggc gggctgtgga aatggaaccg ggagtttcag
     4501 ccgctcctgc cctacctcgc cagccaggcg aacagcggca ggatccggct gggcggcttc
     4561 gatttccagc aaggcggccg cgaacaggat ttcagcaaca ccggggtcgt ggccgagctg
     4621 acggcagagc tcccgccgga gcggcgccag acctgccgcg ccctgtttcg cgacctggtg
     4681 ttcaagggct ccagcccgga gcggcgacag gcggttgcac cctgcctgac ggagatgggc
     4741 gcactgccgc cctccgccga tcgcacggtg cggcgtgagc gccgcgaatt gctggccaac
     4801 ctggccgcct tcaccgccgc ggatccgtcg gacagcaccg gctataccgc ccagcgcgac
     4861 cgcgcgatgt ttgccaattt cgaacgctgg aaagcgcgct ggccggccgg ttccaaaatc
     4921 atcgtctgga ccgccaccgc ccatgccgcc cgcgccccgc acgggcacaa gggctttcaa
     4981 ggggtgcagc cgttcggcgc ctatctcgcc gggcgttacg gccaggcggc tttcgccctc
     5041 ggctttgccg cgcaaggcgg aaccacccgc ggccggcccc agccccgcgt gatcgcgccg
     5101 cccgcgcccg gcacgctgga ggcctgggcc ggaccgatcg caccgggcgg ggcgctgttt
     5161 ctcgaccgtg cggccctggc ccgggccggg gaaaggcccg ccgggctgtt caaccatggg
     5221 ccggtcacgg ccacctggtc gcgcctgctt gacggcgtgg tcgtgtttga cgccgaacga
     5281 gcccccgccg atatccgcaa aggcggctga ccgcagaggt tgcaaggcgc gtggggttct
     5341 gcttgggaca gggcatgagc cacgaaccgc acgacaagct tcagtccgcc gccgatgtgc
     5401 ccggcacgtc gcgcctggtg gaaatgaccg ccggcgaatg ggccggctgg taccactggg
     5461 aaccggtcga cgatttcgag gagcacaccg ggccgtttta ctgcaagccc gatggcgacg
     5521 ccctggtctg cggcttccgg cccgaggcga agaaccgcaa cggcggcggc aatgtgcacg
     5581 gcggggcgct gatgaccttt gccgactatg ccctgttcat gaccgccagc gggatggacg
     5641 ggtcggtcca cggcgtgacg ctgaccatga actgcgaatt cgtcggcccg gccgagaccg
     5701 gccgcctgct gaccgcgcgg ggcgagatcg tccggcaggg cttctcgatc gtcttcgtgc
     5761 gcgggatcat cgacgacgag gggcgtccgg tgctgacctt ctctggcacg atcaagcggc
     5821 tgaccccgcg cgagaagcgc tgactatgct gcggcgcaca acgcgccgct tgcactggcg
     5881 cggaatttcg cggataaggc tggcaaatca cgccttcccg tggagtttcc gaccgtgacc
     5941 aagacctcgc agcccacccc gcgctggacc aagcccgagc tggaaaagct cggcactctc
     6001 aaggatgtcg ccggtgggac ctttgccggc aacgattcca acggccaggg ccagggatcg
     6061 aaccgcaacc ccgtttccta agcccacccg caccccatcg gtgtgctggc ctttgctcgc
     6121 ggctgggatg atttcatccc cgatgcggcg cgaatggcgt cgtcgctggg cttgccggct
     6181 cctgaaaccc tacgctttca ccgcgacgga gcggccggtt acgccctgac tccggggctg
     6241 ttcggcgcgc cggccggccg gccgggccgg attccggttc ccgccatcct gccttctggc
     6301 gaccgtgtgc tgttcaccgg ctggtttgac aaccgcgccg ccatcgccgc ccggctgggc
     6361 ctgcccgatg ccgatccggc cacggtctat ggccacgcgg tagcgcgctg gggcgatgag
     6421 gccgagtgcc acgtgatcgg cagctatgcc gcggtggtcg accggcccgg gcgcggcgag
     6481 gtgcgcctgg cccgcagccc gttcagcgcc ccgccgctcc atttccacca ctccgcccgg
     6541 atggccgcgg cggccagcgt cccgcgggcc ctgttcgcgc tgggcctgcc ggccgagctc
     6601 gacgaggagc ggctggccat gcacctccac gccgccgtga ccgaccggcc gggcggctgg
     6661 tacaagggga tcgaggccct gcaactgggc gcggtcgcca ccctgacccg cgccggggtg
     6721 cggcgggccc ggccctatga ttccgccgca aacccgccgg tgcggctgaa gcgcgatgcc
     6781 gattacgtcg ccgcggccga ggccttgctg gacgaagcga tcgacgcgac cctgccgggc
     6841 tttcgccagc ccgcaactct gctgaccggc gggctcgatt cgaccctggt cgcccgccac
     6901 ctgctggacc ggatgccggc ggaccagcgc ctgcccagct tcacctggac ctgcgagccg
     6961 ggccatgaag gccgcgacag cgccttccac tttgccgacg agcggccgcg ggtcgaggcc
     7021 tttgccgcga tgcacccccg gatcgagccg cacttcctcg acaacgccgg gctgggcttt
     7081 gacgacggga tgcaggacct gttcctgctg gccggagtgg cgccggcggc gatcggcctg
     7141 atctatccct accacggcgc gctggccgcg gcccggcagc gcggctgcga cctggtgatc
     7201 ggggccggcg ggggcaacac caccttctcg gccgaggggc gcagcgccta taccgaatac
     7261 ctgctgagcg ggcgctggcg ccagctgtgg cgggccctgg ccgcccgccc gggggacacg
     7321 cgcccggtct ggcggcggct ggtctcgctc tccttgctgc gggccctgcc agaaccgctg
     7381 tggcgcgcgg caagccgctg gcgcggggcg atcccgggca atccgcaccg gaccttcggg
     7441 gcgctcaatc cggcctggcc cggccgcgcc gcgctggaac gccaggccgc cgcggacgat
     7501 ccgggcctga cccggccgtt ctaccgcacc cgcgccgaag aggtccgcgc catcgccgcc
     7561 cagaccgatg ccgacgggtt cgacctgatg cagggcctgc agcagaagta ccggatcgcg
     7621 ttccgcgacg tgacgctgta ccgcccgctg gtggagttct gccggggcct gccggtcgat
     7681 cagctgatgc gcgacgggga aagccgctat ctggcccggc ggatgggcca ggggcgcctg
     7741 cccgaggcga tccgcaccga gacccgctat ggcctgcagc acggcgactg gcactggcgg
     7801 atcggccagc gccgcgcggc cctgctggcg gagctgcgcc agctgcgcga ggatccggcg
     7861 gtgggccgaa tggtcgacgt cgggcgcctg atcgccttgc tcgaggcctt tcccgacagc
     7921 cccggcaccg atccggacct ggcctatcaa taccagattg ccttgccgag cgggatcgcc
     7981 gcggcccgcc ttatccgcta tgtccgcggc accaatgcct gatgccccgc ctttgccgcc
     8041 gcgcctgatc gggccggagc accaccgccc gatcgccgcg ctggtcgcgc tgatgaccct
     8101 tgccgccgcg accgaggggc ttggcatcat cctgcttgtc ccgctgctcg gcgcgctggg
     8161 ccagggcgat tccggatcga tcggggcaat gctgcggacg atcggcctgc cgctgcggct
     8221 tgatctgctg ctggcgctgt ttgtcgggct ggtggcgctg cgggccgtgg tggtccacct
     8281 gcggaccctg gccgggatgc ggttcgagac ggcggtggtc gatgccttgc gccaccgcgc
     8341 ctgggccgcc ctgctgcact gcgaatggcg cgccctgctg ggaatgagcc gctcgaacac
     8401 cgccagcctg ctgatcaacc gggtcgaccg ggccggaacc tatgtcaacc agggcatcct
     8461 ggcggcctcg acgctggtca cgctgggcgg ggtggccctg gccgccctgg cgatcgcccc
     8521 gctgctgacc gccggagcgg tgctggccgg ggccttggtg ctgctggcct tccacgggat
     8581 gcggcggcgg gccgcggcgc tgggcgatgc tctgggcaag gcctatgccg gggtccacgg
     8641 ccggctcgac gaagggctgg gcgccctgcg cgcgatcaag gggctgggcc gggaggacaa
     8701 ggccctgacc gatctggtgg gcgaattcgc cgggctgcgc gaaacctcgc tggcctatct
     8761 gcgcgatgtc gggcgcgggc agatcgtgct ccaggccggc ggcgcggccg tgctggccgt
     8821 gctggtctgg ctggcgctgg aacgctggca cagcgatgcc gcggtcgtcc tgccgatggt
     8881 ggcgctgttc gcccgggccc tgccgctgat cggcaccctg cagcaggccg ggctcaacgc
     8941 cgcccatgcc cgcccggcgg tggacgaggc gctggccctg attgcccggg ccgaagccgc
     9001 acgcgaggcc gatgccgggc ccggcgcccc gccggcgctg gaccgggcga tccggctgga
     9061 aggcgtcacg gtccggttcg aaggcgagcc gcgcccggcg ctcgacacgg tgaccgcgtc
     9121 cattccggcg cgcgggatca ccgcaattac cggcccctca ggcgcgggca agagcacgct
     9181 ggccgacctg atcgggggtc tgctgtcgcc cgacgacggg acggtccgga tcgatgacac
     9241 cgtgctggcc ggtccggccc gccgggcctg gcgcagccgg gtggcctatg tccagcagga
     9301 cccggtgctg ctcgccgcca gcctgcgtga caacctgcgc tgggccgcgc cggaggccga
     9361 tgacgcggcc ctggccgccg cgctgcgggc ggcttcggcc ggattcgcgc tggacctgcc
     9421 gctggggctc gacacccggc tgggcgatgg cggccgcgcg ctgtcgggcg gcgaacggca
     9481 gcggctgatg ctggcccggg cgctgctgcg cgatccggcg ctgctgatcc tggacgaggc
     9541 caccagcgcg ctggaccccg gcaacgaggc ggaaatcgcc gccgcgctgg aggggctgcg
     9601 gcaccgcatg gcggtggtgg tgattgccca ccgcggcacc ctggccgacc gggccgatca
     9661 ggtcatccgg ctggagaacg gccggatcgc ggcctagccg tagacctcgg gcagctcgcg
     9721 cggggcctgg gcctcggcca gttcgtaagc gcgcggatcg tcgacatcga gccaccaggc
     9781 ttcgccgata tccatcgtcg ccgcgcggcc ggcatcagcc aggatctgca tcccttcgga
     9841 caggcttccg gccttgccct gggcaatcgc ggtgcggatc gcctcggcca gttcgggcgt
     9901 gcacaggaac gccccgcaat cgaccgcgtc ataggggtcg atggtcttgc cgatcgcccg
     9961 gatgaacccg gcttcgtccc gtttcaccca ggtggcgtcg tcgggatcga tcagcggatg
    10021 atcgcagcgg gtgtcgatcg ccagggtcac gccccggtcc ggcccgtgct gctgcgccag
    10081 ccgggtcagg atcgatcccg aaaagatgtg gtcgctcatc atcagcaggt aattgccggg
    10141 aatcactgcg gccccggcca gaaccgacca gccgttgggc ttcgaccagt cctcgacccg
    10201 ctgcgccacc accgggatgc cggcgcgctc ggacagggcg ggcagggccg cctcgagccg
    10261 gtcggcctcg tgcccggtca ccaccacgac ccgctccacc ccggcggcct tggcctggcg
    10321 cacgcccagt tcgagcagcg ggatgcccgc aatcggggtc atcggcttgg aatccgaaat
    10381 gtcccgcagg cggctgccga acccggcggc gatgatcagc gcgtccatct gatgatcctc
    10441 atacagaaac ggcgccagtt tcccggcgcc gcttccaata cctgtccgtt cgcgcttatt
    10501 cggcggcgag cggggcttcg gccacgtatt cgtcaaccat ctgggcggcc agatcgtcgt
    10561 tgaactgctg cggcgggtgc ttgcagaaat aggccgacgg gccgatcagg gccccgcctt
    10621 cgccgcgctc cagcgcgacc ttggcgcagc ggatcgcgtc catgacgcag gccgccgagt
    10681 tcgggctgtc ttcaaccgag aggcgcagtt ccagttccat cggcacgttg ccccactgca
    10741 ggccttccag gcgcaggaag cacagcttgt tgtccttctg ccagggaaca tagtccgacg
    10801 ggccgacgtg gatgttttcg tcttcgaggc gctgggcgag catcgcctgg acggcttcgg
    10861 tcttcgattc cttcttcgaa cccagacgct gacggtccag catgttcatg aagtcggtgt
    10921 tgccgccggt gttgagctgg taggtccgct caaccgtcac gccgcgggcg gcgaacaggc
    10981 tggagagcac gcggtggacg atcgtggcgc caacctgggc cttgatgtcg tcgccgacga
    11041 tcggaatgtt cttttcgcgg aagcgggctt cccattccgg cttgctggcg atgaacaccg
    11101 gcatgcagtt gaccacggca acgccggctt ccagcgcgca ttccatgtag aattcggtgg
    11161 cttcctgcga gccgaccggc aggaagttga tcagcacttc cgcgcccgaa tccttgatcg
    11221 cctgaacgat gcgggcatgg tcggcctcgg cttcgtccga aacgatgaag cccttctcgc
    11281 ccacggtggt catgtgggcg gcaacgccgt ccgagacctt gcccatgatc accttcgcgc
    11341 cggtggccgg cacggtgggc atgaacacct tggtgttgtt gggctgggca aagatcgctt
    11401 cggcgatgtc cttgccgacc ttgcgggcgt cgacgtcgat gccgagcacg aagtccacgt
    11461 cacccgcgcc atagccgccg atcttgtcgt ggatcaggcc ctgcgaggaa ttgttctggc
    11521 ggtaataggc gacgccctgg acgagcgagc ttgcgcagtt accgacgccg atcatggcga
    11581 ccttgatagg cttcattctg tttctgtccc ttttcgagcg gcacgacccc gaccgcgaaa
    11641 ggccgggtat tggaaaaatc ggtcgccaaa agcaagccaa ttggtccgcg tcaagccgaa
    11701 tgcgcgcttt cgccccccgg tgcgacattt ctgttcgcag cggcaaggcg gcggttgcgc
    11761 tcggcgcaga gggccttatc ccctcgaatc gcacggaatc actagggagt aacacgggtg
    11821 gcaacgggga atcagggggc tggaacggcg atgcagcggc cgcagcgccc gcgcgaactg
    11881 caggacccgc tgaatcacta tctctaccac ccgctggcct ggcagttcgc ccggctgctc
    11941 gcgccgactc cgatcacccc caacatggtc tcggtcctgg gggccggatt cgtggtggcg
    12001 gcggcctttg cctataccca gctgacctgg ccggtttcgg ccgcgctggg gatgatcctg
    12061 cacatgacct ggcacgtggt ggacggggcc gacggcgatc tggcgcggat gaccggccgc
    12121 tccagcccga tcggcgaaat ggtcgacggg ctgtgcgact atctgagcca cgcggtgctc
    12181 tatatcgcgc tcgccatcct gctggcgggc ggcgcggggg tcttctcggg ctgggcggcc
    12241 tgggtgctgg tgatcggtgc cgggatcagc cacgcggttc aggccaacca tgtcgaggtg
    12301 cagcgccgcc agtaccagtg gtgggtctat ggcacggcct ggatccgcaa ttcccacggg
    12361 ggcagcgatt cggccacctc gcga
//
LOCUS       DASUDV010000253         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2195334,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000253 DASUDV010000000
VERSION     DASUDV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195334"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFV30_05485"
     CDS             complement(<1..382)
                     /locus_tag="VFV30_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4847574.1"
                     /translation="MTDTAPLTGPLAGLKVLDLSRVLAGPWCGQMLGDLGAEVTKVEQ
                     PGQGDDTRRWGPPFLPDGSRDSAYYLCVNRNKRSIAIDISRSEGADLVRALAAQADVV
                     LENFRVGGLAKYGLDYASLKAIKPD"
     gene            520..1422
                     /locus_tag="VFV30_05490"
     CDS             520..1422
                     /locus_tag="VFV30_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX4847575.1"
                     /translation="MTGITILDGGMGRELKRIGAPFRQPEWSALAMIEAPEAVLAVHR
                     SYVLAGAQVITTNSYALVPFHIGEERFASLGEALADRAGKAARQAADEAGADRVRVAA
                     SLPPLFGSYRPDLFNPDEAARVLAPLVAGLAPHADLWLAETLSATAEARVVADMLKVD
                     HRPLWLSFTLEDSAEDAAANPRLRSGEPVARAVDLAIDSGAAALLFNCSQPEVMEGAV
                     LAALAAMKERGGSIPVGVYANAFPPQPKEATANETLLPIREDLDPDGYAAWIERWLGA
                     GATIVGGCCGIGPEHIARIAQLRG"
     gene            complement(1419..>2006)
                     /locus_tag="VFV30_05495"
     CDS             complement(1419..>2006)
                     /locus_tag="VFV30_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847576.1"
                     /translation="LHWEAAEEFGRRYPRIPVSVELYERHGRLFTCAGGTAAMDMMLD
                     EIAGSHGISLANAVSDQLIHERIRPPSSPQRGSQAERTGVTNPLVQRLVKRMERGIAR
                     GESLAAILAAEAISRRQAERLFEAQIGKPPGTFYRELRIRHAVELMRNARLPVHEAAL
                     AAGFSSQPVFSRACKQAFAQTPRELSNKPNLPWDD"
BASE COUNT          327 a          641 c          688 g          350 t
ORIGIN      
        1 ggtcgggctt gatcgccttg agcgaggcat agtccaggcc atacttggcc agaccgccga
       61 cccggaagtt ttccagcacc acatcggcct gtgctgccag tgcgcggacg agatctgctc
      121 cttcgctgcg cgaaatgtcg atcgcgatcg aacgcttgtt gcggttgacg cacaggtaat
      181 aggccgaatc gcgtgatcca tcaggcagga acggcgggcc ccagcggcgg gtgtcgtcgc
      241 cctggccggg ctgctccacc ttggtcacct cggcccccag atcgcccagc atctgcccgc
      301 accatgggcc ggccagtacc cgcgacagat cgaggacctt gagcccggcg agcggtccgg
      361 tgaggggggc ggtatcggtc atgggcgggg caatcatgct tgaggaatcg agccgctgcg
      421 ctagcgatcc gccggttgct tcgtcaaggt gaagcgggtt cgcctgcccg ccatcgcggt
      481 tgacggatcg cgtcggtgtc cggatagact ggccccggca tgacaggaat cacaattctc
      541 gatggcggca tgggccgcga actcaaacgg atcggcgcgc cctttcgcca acccgaatgg
      601 tcggccctgg ccatgatcga agcgcccgaa gcggtgctgg cggtgcatcg ctcctatgtc
      661 ttggccgggg cacaggtgat aaccaccaac agctatgcac tggttccctt ccatatcggg
      721 gaagagcgct ttgccagtct gggcgaagcc ctggccgatc gggccggcaa ggcggcacgc
      781 caggcggctg acgaggccgg tgccgatcgt gtcagggttg cggcgagcct gccgccgctg
      841 ttcggatcat accggcccga tctgttcaac ccggacgaag ccgcgcgggt tctggcgccg
      901 ctggttgccg ggctcgcgcc gcacgccgat ctgtggctgg cggaaacgct ttcggccact
      961 gcggaagcgc gggttgttgc cgatatgctg aaagtggatc accggccctt gtggctctca
     1021 ttcacgcttg aagatagcgc cgaagacgcc gccgccaatc cgcgcctgcg ttcgggtgag
     1081 ccggttgcca gggccgtaga cctggcgatc gatagcggcg cggcggcgct gctgttcaat
     1141 tgcagccagc ctgaagtgat ggaaggtgcg gtgttggccg cgctggcggc gatgaaggag
     1201 cggggcggct cgatcccggt cggggtctat gccaacgcct tcccgcccca gcccaaggag
     1261 gcgactgcca acgaaaccct gctaccgatc cgcgaggatc tcgatcccga tggctatgcc
     1321 gcctggatcg aacgctggct tggcgcgggg gcaacgatcg tgggcggttg ctgcgggatc
     1381 gggcctgagc acatcgcgcg gatcgcgcag ctgcgaggct aatcatccca gggcaggtta
     1441 ggcttattcg agagttcgcg gggagtttgg gcaaaggcct gcttgcaagc ccgcgagaag
     1501 actggctgcg atgaaaagcc ggcggccagg gcggcttcgt gcaccggcag gcgggcattg
     1561 cgcatcagtt ccaccgcgtg cctgatccgc agttcgcggt agaaggtgcc gggcggcttg
     1621 ccgatctgcg cctcgaacaa gcgttcggcc tggcgccgcg agatcgcttc ggccgcaagg
     1681 atcgcagcga ggctttcgcc ccgggcgatc ccgcgctcca tccgcttgac caggcgctgg
     1741 accagcggat tggtaacccc ggtgcgctcg gcctgcgatc cgcgctgcgg cgaactgggc
     1801 gggcggatcc gttcatgaat caactggtcc gacactgcat tggccagcga gatcccgtgg
     1861 ctgccggcaa tctcgtcgag catcatgtcc atcgctgccg tgcccccggc gcaggtgaac
     1921 agccgtccgt ggcgttcata cagttccacc gaaaccggaa tccgtggata gcgccggcca
     1981 aactcctctg ccgcctccca atggag
//
LOCUS       DASUDV010000254         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1201263,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000254 DASUDV010000000
VERSION     DASUDV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201263"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV30_05500"
     CDS             complement(<1..336)
                     /locus_tag="VFV30_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4847577.1"
                     /translation="MKRLIASVLLAALPLVAATAQVAPAPTAPATTPIPATDFARIPY
                     VSRPELSPDGLYLGGLMGIEGRQVIAIVSLFDKAKPVVLALPEDNQAAWVRWVNNDHL
                     IVGIRALMPV"
     gene            complement(336..1688)
                     /locus_tag="VFV30_05505"
     CDS             complement(336..1688)
                     /locus_tag="VFV30_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX4847578.1"
                     /translation="MTAATEKQQATVWGHPKGLVFLASTEMWERFSFYGMQALLTLYM
                     VKQLLLPGFAEQVWGLAALRSLLGGGGPISDTDFAGLIAGFYSGLVYFTPIVGGWIAD
                     RLLGAKRTVLIGVLLMSAGHLAMTFDQLFLVALVLLILGSGALKGNISAQVGTLYPPE
                     ARSMRDRGFSIFSTGINIGAVSGPLVTGAVAAIWGWHAGFAVAAALMLVALVTYLAGQ
                     RHFPEAPPRRADPEAKPLTKAEKRKVWALIAVIALTIPAEIAYPMVWTIGAVWVDSYV
                     ALGNIPAPWFGSADSLGAILAAAPLVALWAAQARKGREPSSVGKIAIGTGLTGVGAMI
                     LAAGSATQTGPDTINIIWPILGYFTMGLAWMYYWPTTLSLVSRVAPPAVAATLVGGAF
                     FSPFIAHTLAGVIGTRFDNMTPAAFWAMDGAIGLAGAVLLFALKNPINRVIEAKAEAD
                     "
     gene            complement(1752..2231)
                     /locus_tag="VFV30_05510"
     CDS             complement(1752..2231)
                     /locus_tag="VFV30_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX4847579.1"
                     /translation="MKGEGPPITPAGMAALRARYDHLLGTERPAIVEIVSWAAGNGDR
                     SENGDYLYGRKRMREIDRELAHLARRMKVARVIDPARQEDRARVWFGAEVTIADEDDN
                     HRTLTLVGDDEQDASAGRIGWSAPLARALRGAAVGDLRRVVLPGGEKEWEVLKIVYP"
     gene            complement(2228..>2873)
                     /locus_tag="VFV30_05515"
     CDS             complement(2228..>2873)
                     /locus_tag="VFV30_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4847580.1"
                     /translation="QAQSEADHVLVADLARELGRRDLGVILGQAAHADGHGVFRKTSF
                     PLIPVPQGTNWTMVHAITRQESQFAQNAMSHAGARGLMQLMPGTAREQAGKMAMSYEQ
                     QALVGDAGYNLMLGDGYFRRMLDYYGGSHPLAVAAYNAGPGNVNAWLRANGDPRNGGP
                     DWVEWVEKIPISETRNYVQRVLENAVVYDAMNPARAGYRGANPLSHYLGKRYPG"
BASE COUNT          506 a          992 c          919 g          456 t
ORIGIN      
        1 aaccggcatc agcgcccgga tcccgacgat cagatgatcg ttgttgaccc agcgcaccca
       61 ggccgcctgg ttgtcttcgg gcagggccag gaccaccggc ttggccttgt cgaacaggct
      121 gacgatcgcg atcacctggc gaccttcgat ccccatcagc ccgcccaggt aaaggccatc
      181 gggggaaagc tcgggccggg agacataggg aatgcgggca aaatcagtcg ccgggattgg
      241 cgtggtggct ggcgcggtgg gggcgggcgc aacctgcgcc gttgccgcaa cgagcggcag
      301 tgccgccagc aggaccgatg cgatcagccg cttcatcagt cggcttctgc cttggcttcg
      361 atcacccggt tgattgggtt cttcagcgcg aacagcagca cggcgccggc caggccgatc
      421 gctccgtcca tcgcccagaa cgcggccggg gtcatattgt cgaaccgggt accgatcacg
      481 ccggccaggg tatgggcgat gaagggtgaa aagaacgcgc cccccaccag cgttgccgcc
      541 accgccggcg gcgccacccg gctgaccagc gaaagcgtgg tcggccagta gtacatccag
      601 gccagcccca tggtgaaata gccgaggatc ggccaaataa tgttgatcgt atcgggcccg
      661 gtctgggtgg cgctgccggc cgcaaggatc attgccccga ccccggtgag cccggtgccg
      721 atcgcgatct tgccgacgct gctcggctcg cggcccttgc gtgcctgggc cgcccacagc
      781 gcgaccaacg gcgccgcggc gaggatcgcg ccaagcgaat ccgccgagcc gaaccaggga
      841 gccgggatgt tgcccaatgc aacatagctg tcgacccaga ccgcgccgat ggtccagacc
      901 atgggatagg cgatttcggc cggaatggtc agcgcgatca ccgcgatcag cgcccagacc
      961 ttgcgctttt cagccttggt cagcggcttg gcttcgggat cggcgcggcg cggcggggct
     1021 tcggggaaat ggcgctgccc ggccaggtac gtgaccagcg ccaccagcat cagtgcggcg
     1081 gccacggcaa agcccgcgtg ccagccccag atcgccgcaa ccgcgccggt gaccagcgga
     1141 ccagacaccg cgccgatatt gatcccggtc gaaaagatcg aaaagccgcg atcgcgcatc
     1201 gagcgggcct cgggcggata gagcgtgccg acctgggccg aaatattgcc cttgagcgcg
     1261 cccgaaccca ggatcagcag caccagcgcc accaggaaca gctggtcgaa ggtcatcgcc
     1321 aggtgacccg cgctcatcag cagcacaccg atcagcacgg tgcgcttggc gcccagcaac
     1381 cggtcggcaa tccagccgcc gacgatcgga gtgaaataga ccaggcccga atagaagccc
     1441 gcgatcagcc cggcaaaatc ggtatccgaa atcggtccgc cgccgcccag caggctgcgc
     1501 agcgcggcca ggccccagac ctgttcggca aatccgggca gcagcaactg cttgaccatg
     1561 tagagcgtga gcagcgcctg catcccgtag aacgagaagc gttcccacat ttcggtcgat
     1621 gcaaggaaca ccagaccctt ggggtgaccc cagacggttg cctgctgctt ttcagttgcc
     1681 gccgtcatcc cgctctcccc tttgcgcttt ggctgcaacg atagagccaa accaagcact
     1741 tgcaagctgg ctcagggata gacgattttc agcacttccc attccttctc gccgccgggc
     1801 aggaccacgc ggcgcagatc gcccaccgcc gcgccccgca gtgcgcgggc cagcggcgcg
     1861 ctccagccga ttcgcccggc cgatgcgtcc tgctcgtcat cgcccaccag ggtcagggtg
     1921 cggtggttgt cgtcctcgtc ggcgatggtc acttcggcgc caaaccagac ccgggcgcga
     1981 tcctcctggc gggcggggtc gatcacgcgg gccaccttca tccgccgcgc caggtgcgcc
     2041 agttcgcggt cgatctcgcg catacgcttg cggccataaa ggtagtcgcc gttctcgctg
     2101 cgatcgccat tgcctgccgc ccacgacacg atctcgacga tcgcggggcg ttcggtgcca
     2161 agcaggtgat catagcgcgc cctgagcgca gccatgccgg cgggcgtgat cggcggacct
     2221 tcgcccttca tccggggtaa cgcttgccca ggtagtggct gagcggattg gcgccgcgat
     2281 agccggcccg ggccggattc atcgcatcat aaaccaccgc gttttccagg acccgctgga
     2341 catagttgcg ggtttccgaa atcgggatct tctcgaccca ttcgacccag tcgggcccgc
     2401 cattgcgcgg atcgccgttg gcgcgcaacc aggcgttcac attgccgggc cccgcattat
     2461 aggcggccac cgccagcggg tgactgccgc catagtaatc gagcatccgg cggaaatagc
     2521 catcgcccag catcaggttg tagccggcat cgcccaccag cgcctgttgt tcatagctca
     2581 tcgccatctt gccggcctgt tcacgcgcgg tgccgggcat cagctgcatc aggccgcgcg
     2641 cgccggcgtg gctcatcgcg ttctgggcaa actggctttc ctggcgggta atggcgtgaa
     2701 ccatggtcca gttggtgccc tgaggcaccg gaatcagcgg gaagctggtc ttgcggaaca
     2761 ccccgtggcc atcggcatgc gccgcctggc ccaggatcac ccccaggtcg cgccggccca
     2821 gttcgcgtgc cagatcggcc accagcacgt gatcggcctc gctctgcgcc tgg
//
LOCUS       DASUDV010000255         6940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1443977,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000255 DASUDV010000000
VERSION     DASUDV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443977"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFV30_05520"
     CDS             <1..838
                     /locus_tag="VFV30_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4847581.1"
                     /translation="AKHQLIAFGTQEADEDRAWELSLMSELDAAIDAGDIWVAYQPQF
                     SLKTSGITGVEALVRWQHPERGAISPEAFIAPAEAHNRMLRLTLNILRQATIACRPII
                     DANPGFRLSVNLSASLLEHPQLADKIGAILFMTGFPASSLTLEVTESAPFSEHAAVTA
                     NLNELAGIGIELSIDDYGTGNATLEYLRTVPCQEIKIDRRFVSGLTTNASDRLLVEST
                     IELAHGLGRRVIAEGIEDQATLERLRAAGCDIAQGYFLAKPMRIEALVKLVADHGNTR
                     AA"
     gene            complement(1259..3151)
                     /locus_tag="VFV30_05525"
     CDS             complement(1259..3151)
                     /locus_tag="VFV30_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HEX4847582.1"
                     /translation="MCGIAGIFHLATPKPVDPARIERMCAAMAHRGPDGQGVWTAPGV
                     GLGHLRLSIIDLAGSPQPMASTDGRAMLVFNGEIYNYRELREELRAAGAQFHTDGDSE
                     VILAAWQRWGPDCLPRLHGMFAFAIYDTEQRTLFLARDRLGVKPLFYAPLSDGSLAFA
                     SELKGLVAHPLLRRDIDPLAVEDYLAWGYVPDHRSILAGVHKLPAGHSLLLRHGAPLP
                     APRQWWDISFAERRKGRAADLEAELLHLLRQAVTSRMVSDVPLGAFLSGGVDSSSVVA
                     LMAEASASPTKTCSIGFDVESLDETAYAGQVAKLFGTDHRARQVSPNDFEHVDTLAAM
                     FDEPFADASALPTWRVCQLARETVTVALSGDGADEAFAGYRRHKFHHAEDRLRSLLPQ
                     GLRGPVFGALGAVYPKADWAPRPLRAKATFQSLAQSSEAGYARAVGVTPPELRQTLYS
                     PDFLALRGDYRAEQAWEDTMRRAPARSGLDRAQYADLKFWLPGDILTKVDRTSMAVSL
                     EAREPLLDHRLLEFAATLPEGLRMHRGQGKWLMKRAMRRYLPDDILFRQKQGFVTPLT
                     DWFRGPLAGAAREIGSGAALARTGWFDSARLSALADSHITGRSDHARLLWQLLMLERS
                     LSKLGV"
     gene            complement(3163..4686)
                     /gene="xrtA"
                     /locus_tag="VFV30_05530"
     CDS             complement(3163..4686)
                     /gene="xrtA"
                     /locus_tag="VFV30_05530"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HEX4847583.1"
                     /translation="MGLSALSPAWRAACRHLLLAWLALIAVFHRDWAAMFAQWWDSST
                     YNHILLVPVIVAWLVWQRLPEVLQLEPRVWRGGLVAFALCVFGWVLGSFADFNLLRQA
                     AAVAMLPATVLLLLGPRLCAALLFPLCYMAFLVPFGDELVPPLQTITAALTIALVNLS
                     GVPASIDGVFIDTPAGLFEVAEACSGVKFLIAMVALGVLVANVGFKGWGKRAAFLVLC
                     VVVPILANGVRAWGTIFAAQYVGVERAAGIDHLIYGWIFFAVVIAAVLGLSWRFFDRS
                     LDEPMIDAAGIAADSRFDRFERAIPAPRLALAAAALTVAVGLGWSALAMNLSAPLPRQ
                     VFLPEVPGWQRTDYAPRAPWEPLAGGADHRLLGSYTDGRGRRVDVFFALYAKQGEGKE
                     AGGFGQGALRPDTGWAWTSNGPAVAAGRSERLIHNGTVERLAYTWYRSGDLLTGSNMQ
                     LKLANIRDRLMLRARPTATLILSAEQAPSGDAKAALADFQAAVGPLGPWMDRIGEAR"
     gene            complement(4709..5947)
                     /locus_tag="VFV30_05535"
     CDS             complement(4709..5947)
                     /locus_tag="VFV30_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HEX4847584.1"
                     /translation="MSGEVLFLAHRMPFPPDRGDKIRSSNILKHLAGLAPVHVATFAE
                     TDADLAQETQLAGVATSHCLVRRTKPLPLAGAEALLRGLPVSLTAFHSPRIAAYVRQV
                     LAERPITAIYVFSGQMGQYVPADFAGRVILDFVDADSAKFEAYAQDGSGPRAWIDGRE
                     GRLLLREEARLAGRAGVSLLATDEEAALFSARLSPSERAAATVQTLRNGIDSVAFDPA
                     QVAPEPQLRAQPGPRLIFTGQMDYAPNIAAVERAVHRLMPAIRAVHPDAGFHVVGRNP
                     AEAVRRLNGLNGCTVWGEVPDIRTWLAAADLALVPLDIARGVQNKVLEAMAMALPVVL
                     SPGAATGIGASDGAHLLIADRDAALVEAVLALLADPARARSLGAAAREFVVAEASWAA
                     AMAPLAGLIGAGEAEARRAA"
     gene            complement(5944..>6940)
                     /locus_tag="VFV30_05540"
     CDS             complement(5944..>6940)
                     /locus_tag="VFV30_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEX4847585.1"
                     /translation="RFVTEHPGGTAFHRPTWLTAVARGTGNRAVALVAERQGALTGYL
                     PLTEAHSPLFGRLLVSSGFGVEGGILADDEGAGKALIAAAEELALRRSCPAIELRGGL
                     LPAERTGWRIRHDSHCGYSAPLAADDEAQLTWIPRKQRAEVRKGLAGELEVSVGTAPE
                     DRAAHYGVYAESVRNLGTPVFPRKLFEAVLDAFGGDADILTVRHAGQPVASVLSLYHK
                     GAVMPYWGGGTWDARRLRANDRMYFELMLHARRRGCDRFDFGRSKTGSGAGDFKKNWG
                     FEGAALGYASWTAPGAEARDADPTSASHAAKIALWKRLPLPLANRIGPLIARGLA"
BASE COUNT         1194 a         2440 c         2347 g          959 t
ORIGIN      
        1 ggccaagcac cagctgatcg ctttcggcac ccaggaggcc gacgaggacc gcgcatggga
       61 actctcgcta atgagcgagc tcgacgcagc catcgacgcc ggcgacatct gggtggccta
      121 tcagccgcaa ttcagcctca aaacaagcgg aatcaccggg gtggaggcgc tggtacgctg
      181 gcaacacccg gaacgcggcg cgatctcgcc ggaagccttc atcgcacctg ctgaagccca
      241 caaccggatg ttgcgcttga cgctcaacat cctgcgccag gcaaccattg cctgcaggcc
      301 gatcatcgat gccaatccgg gtttccggct tagcgtcaac ctgtccgcca gcctgcttga
      361 gcatccgcaa ctggccgaca agatcggcgc gatcctgttc atgaccggct tccccgcgtc
      421 cagtctgacc cttgaagtga ccgaatccgc gccgttcagc gagcacgccg cggtgaccgc
      481 gaacctcaat gaactggccg gcatcgggat cgaactctcg atcgacgact acggcaccgg
      541 caacgcgacg ctggaatacc tgcgcactgt accgtgtcag gaaatcaaga tcgatcgccg
      601 cttcgtctcg ggcctgacca ccaacgccag tgatcggctg ttggtggaat cgaccatcga
      661 actggcccat gggctgggtc gccgggtgat cgccgaaggg atcgaggatc aggccaccct
      721 ggaacgcctc cgcgcagcgg gctgcgacat cgcccagggc tatttcctgg ccaagccgat
      781 gcggatcgag gcgcttgtca agctggttgc cgaccacggg aacacccgcg cggcctgacc
      841 gggcaaccga atcgcgcttt tcaaagccgc aatccaggtg tgcaaggcgc gcccggacgg
      901 accgcgcgtg tctcagaatg agacactttg cgacacccta acaacctgaa atcgagagcg
      961 gaatcgcgcc accggcacgt gccttacaag gccactcgcg cctttgcgcg gttaatgcag
     1021 gttgctttgg caccccctcc accaacagtg tggttaagaa cttcttcttg tctttcctta
     1081 aaaaattact aacaatcggg gatgacaggt tccttggggg gacctgcgtg gtgacaggag
     1141 aatgaccatg tgggcatggt ggcgtactta tttcggtagc ccgtaaggca ttgccgaaac
     1201 cgatattcgg ccccgggatt ccggggccgt tttcgtttgt gcggaaggtg ttagacgctc
     1261 agacgcccag tttgctgagc gagcgttcca gcatcagcag ctgccacagc agccgggcgt
     1321 gatccgagcg gcccgtgatg tgcgaatcgg ccagcgcgga caggcgcgcc gaatcgaacc
     1381 agccggtccg cgccagggct gcaccggatc caatctcccg tgcggcgccc gccaagggcc
     1441 cccggaacca gtcggtcagc ggcgtgacaa agccctgctt ctgccggaac aggatatcat
     1501 cgggcagata gcgccgcatg gcccgcttca tcagccactt gccctgcccc cggtgcatcc
     1561 gcagcccttc cggaagggtc gccgcgaatt cgagcaggcg gtgatcgagc agcggctcgc
     1621 gcgcttccag gctgaccgcc atgctggtgc ggtccacctt ggtcaggata tcgccgggca
     1681 gccagaactt gagatcggcg tattgcgccc ggtccagacc cgaccgggcc ggggcgcggc
     1741 gcatcgtgtc ttcccaggcc tgttcggccc ggtagtcccc gcgcagcgcc agaaagtccg
     1801 gcgaatagag cgtctggcgc agctccggcg gtgtcacgcc gaccgcccgg gcatagccgg
     1861 cctcgctgct ctgggcgagc gactggaacg tcgccttggc gcggagcggg cgcggcgccc
     1921 agtccgcctt gggatagacc gcgcccagcg ccccgaacac cggcccgcgc aggccctgcg
     1981 gcagcagcga acgcagccgg tcttcggcgt ggtggaactt gtggcggcgg tacccggcga
     2041 aggcctcgtc cgcgccatcg cccgacaggg ccacggtcac cgtttcgcgg gccagctgac
     2101 agacccgcca ggtcggcagg gccgaggcat cggcaaaggg ttcgtcaaac atcgccgcca
     2161 gcgtgtcgac atgctcgaaa tcgttcggcg aaacctgccg cgcgcggtga tcggtgccga
     2221 acagcttggc gacctggccg gcataggccg tctcgtccag gctttcgacg tcgaacccga
     2281 tcgagcaggt cttggtcggg cttgcgctgg cctcggccat cagggcgacc acgctggagc
     2341 tgtcgacccc gcccgagagg aacgcgccca gcggcacgtc ggacaccatc cggctggtca
     2401 cggcctggcg cagcaggtgc agcagctccg cctccagatc ggcggcgcgg cccttgcgcc
     2461 gctcggcaaa ggaaatgtcc caccactgcc gcggagcggg cagcggcgcg ccgtggcgca
     2521 gcagcagcga atgcccggcc ggcagcttgt gcaccccggc caggatcgag cggtgatcgg
     2581 ggacatagcc ccaggccagg tagtcctcca ccgccagcgg atcgatatcc cggcgcagca
     2641 gcgggtgggc gaccaggccc ttgagctccg aagcgaaggc caggctgccg tcgctgagcg
     2701 gggcgtagaa cagcggcttc acgcccagcc ggtcacgggc caggaacagg gtgcgctgct
     2761 cggtgtcgta gatcgcgaag gcgaacatcc cgtgcaggcg gggcaggcaa tccgggcccc
     2821 agcgctgcca ggcggcaagg atcacctcgc tgtcaccgtc ggtgtggaac tgggccccgg
     2881 cggcgcgcag ctcttcgcgc agctcgcggt agttgtagat ctcgccgttg aagaccagca
     2941 ttgcccggcc atcggtggag gccatcggct gcggcgatcc ggccaggtcg atgatcgaca
     3001 ggcgcaggtg ccccagcccc acgcccggcg cggtccacac cccctgcccg tccggcccgc
     3061 gatgggccat ggccgcgcac atccgctcga tccgcgccgg gtcgaccggc ttgggcgtgg
     3121 caaggtgaaa gatcccggcg attccgcaca tgggacaggc gcttagcggg cttcgccgat
     3181 gcggtccatc cacggaccca gcggacccac cgcggcctga aaatcggcca gcgcggcctt
     3241 ggcatcgccg gatggggcct gctcggccga aaggatcaga gtcgcggtcg gccgggcgcg
     3301 cagcatcagg cggtcgcgga tgttggccag cttcagctgc atgttgctgc cggtcagcag
     3361 atcgccgctg cggtaccagg tataggccag ccgctcgacc gtgccgttgt ggatcagccg
     3421 ctcgctgcgg ccggccgcca cggcggggcc gttgctggtc caggcccagc cggtatcggg
     3481 ccgcagcgcc ccctgcccga aaccgccggc ctccttgcct tcgccctgtt tggcatagag
     3541 cgcgaagaac acgtcgaccc ggcggccgcg gccatcggtg taggagccca gcaagcggtg
     3601 atccgcgccg ccggccagcg gctcccacgg cgcgcgcggg gcatagtcgg tccgctgcca
     3661 gcccggcact tcgggcagga acacctggcg gggcagcggc gccgagaggt tcatcgccag
     3721 cgccgaccag cccagcccca ccgccaccgt cagcgcggcc gccgccagcg cgaggcgcgg
     3781 tgccgggatg gcgcgttcaa accggtcaaa ccggctgtcg gcagcgatac ccgcagcgtc
     3841 gatcatcggc tcgtccagcg agcggtcaaa gaaccgccac gacaggccca gaaccgcggc
     3901 aatcaccacg gcgaagaaga tccagccata gatcaggtga tcgatcccgg cggcgcgctc
     3961 cacgccgaca tactgggcgg caaagatcgt gccccaggcc cgcaccccgt tggccaggat
     4021 cggaaccacc acgcacagca ccagaaaggc ggcgcgcttg ccccagccct tgaacccgac
     4081 attggcgacc agcaccccca gcgcgaccat ggcgatcagg aacttcacgc ccgaacaggc
     4141 ctcggcaacc tcgaacaggc ccgcgggggt atcgatgaac accccgtcga tcgaggccgg
     4201 cacgcccgac aggttgacca gcgcgatcgt cagcgcggcg gtgatggtct ggagcggcgg
     4261 caccagttcg tcgccgaacg gcaccagaaa ggccatgtag cagagcggaa acagcaaggc
     4321 ggcgcagagc cgcggcccca gcagcagcag gaccgtggcc ggcagcatgg caaccgccgc
     4381 ggcctggcgg agcaggttga agtcggcaaa gctgcccagc acccagccaa agacgcacag
     4441 cgcaaacgcc accagccccc cgcgccacac gcgcggctcc agctgcaaga cctcgggcag
     4501 acgctgccag accagccagg cgacgatcac cgggaccagc aggatgtggt tgtaggtcga
     4561 actgtcccac cactgcgcga acatggccgc ccaatcgcgg tggaacaccg cgatcagcgc
     4621 cagccaggcc agcagcagat gccggcaggc cgcgcgccag gccggcgaca gtgccgacaa
     4681 gcccacggag ggagcggcgg gaagcgcgtc aggcggcacg acgggcctcc gcctcgccag
     4741 cgccgatcag cccggcaagc ggagccatgg ccgccgccca gctggcttcg gcaacgacga
     4801 attcccgcgc cgcggcgcca agcgaccggg cccgcgccgg atcggccagc agggccagta
     4861 cggcttcgac cagcgccgca tcgcggtctg caatcagcag gtgcgccccg tcgcttgcgc
     4921 cgatgccggt cgcggccccg ggggacagga ccaccggcag tgccatggcc atggcctcca
     4981 ggaccttgtt ctgcaccccg cgcgcgatgt cgagcggaac tagggccagg tcggccgctg
     5041 ccagccaggt gcggatatcc ggcacttcgc cccagaccgt gcagccattc agcccgttca
     5101 gccgccgcac cgcctcggcc gggttgcggc caaccacgtg gaaaccggca tcgggatgga
     5161 ccgcgcggat cgccggcatc agccggtgca ccgcccgctc caccgcggcg atgttggggg
     5221 catagtccat ttgcccggta aagatcaggc gcggccccgg ctgggcgcgc aactgcggtt
     5281 cgggcgcaac ctgggccgga tcgaaggcca cgctgtcgat tccgttgcgc agggtctgca
     5341 cggtcgcggc ggcccgttcg gacggcgaaa ggcgggcgct gaacagcgcg gcctcctcgt
     5401 cggtggcgag caggctgact ccggcccggc cggccaggcg cgcttcctcg cgcagcagca
     5461 ggcggccttc gcgcccgtcg atccaggccc gcgggccgct gccgtcctgg gcataggctt
     5521 cgaacttggc cgaatccgcg tcgacgaaat cgaggatgac ccgcccggca aagtcggcgg
     5581 ggacatattg ccccatctga ccggaaaaga cgtagatcgc ggtgatcggc cgttcggcca
     5641 gcacctggcg gacataggcg gcgatccgcg ggctgtgaaa ggcggtcagg ctgaccggca
     5701 ggccgcgcag cagcgcttcg gccccggcca gcggcaacgg cttggtccgg cggaccaggc
     5761 aatggctggt ggccacaccg gccagctgcg tttcctgggc caggtccgca tcggtctcgg
     5821 caaaggtcgc aacatggacc ggggccagcc cggccaggtg cttcaggatg ttgctcgacc
     5881 ggatcttgtc gccgcgatcg ggaggaaagg gcatccggtg ggcaaggaac aggacctcgc
     5941 cactcacgcc agcccccggg cgatcaaggg tccgatccgg ttggcgagcg gcagcggcag
     6001 gcgcttccac agcgcgatct tggcagcgtg gctggcgctg gtgggatcgg catcacgcgc
     6061 ttcggcaccg ggcgcggtcc agctggcata gcccagcgcc gcgccttcga agccccagtt
     6121 cttcttgaag tccccggccc cgctgccggt cttggagcgg ccgaaatcga accggtcgca
     6181 gccgcgccgg cgggcgtgga gcatgagttc gaaatacatc cggtcgttgg cgcgaagccg
     6241 ccgcgcgtcc caggtgcccc cgccccaata aggcatgacc gcgcccttgt ggtacagcga
     6301 gaggacgctg gcgaccggct ggccggcgtg gcgcacggtc aggatatcgg catcgccccc
     6361 aaaggcatcg agcacggcct cgaacagctt gcgcgggaag accggggtgc cgaggtttcg
     6421 cacgctttcg gcatagaccc cgtagtgcgc cgcgcggtcc tcgggcgcgg tgccgaccga
     6481 cacctccagt tcgcccgcca gacccttgcg cacttcggcg cgctgcttgc gcgggatcca
     6541 ggtcagctgg gcctcgtcgt cggcggcgag cggcgcggaa taaccgcagt gcgaatcgtg
     6601 ccggatcctc cagccggtgc gttcggccgg cagcaggccg ccgcgcagtt cgatcgccgg
     6661 gcaggaccgg cgcagcgcca gttcctcggc cgcggcgatc agcgccttgc cggccccctc
     6721 gtcgtcggcc agaattccgc cttcaacgcc aaaaccgctg gagaccagca gccgcccgaa
     6781 cagcggcgaa tgggcctcgg tcaggggcag atagccggtc agggcacctt ggcgttcagc
     6841 gaccagcgcc acggcccggt tgcccgtccc ccgcgccacg gcagtcagcc aggtcgggcg
     6901 gtgaaaggcg gtgccgcccg gatgttcggt tacaaagcgt
//
LOCUS       DASUDV010000256         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1311619,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000256 DASUDV010000000
VERSION     DASUDV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311619"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..91)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            283..1425
                     /locus_tag="VFV30_05545"
     CDS             283..1425
                     /locus_tag="VFV30_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX4847586.1"
                     /translation="MSFESRPRDFAARFTRRGARGLGTRRARRHLGRRALVLLAAIAV
                     PAVAAPGEWRPFDIGNAGSADLAMEPMPFERSGESFPGSAFYYLAPTEALPGEIGSFS
                     LISEAAPIGTTDFSGGPVARAIRFDLSGVDRTRALDCLTAAVYYEARSEPDHGQRAVA
                     QVVLNRVAHPAYPNTVCGVVYQGSERTTGCQFSFTCDGSLAKRPNPYFWERSRRVAEM
                     ALSGHVETSVGLATHYHTIQIYPYWAPSLNFITTIGAHRFYSFKGKAGQSGTFRFAYA
                     GGEPMAAPHARNPAADKAPDPVLDPLALQKSYEDGFKASPAGTGLVQPGDVGKAPAKP
                     APPPRYAPEVEKRGGDRQYSGEKLPGSTGVRPEYENSGKWIGQPGG"
     gene            1792..2046
                     /locus_tag="VFV30_05550"
     CDS             1792..2046
                     /locus_tag="VFV30_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847587.1"
                     /translation="MAARIAARAANDNGDGDSHADTLRAALKHFAAYGMAAAGEARDR
                     ARKAHFAGDRDGYRHWLGICRALDRRMAQALVANLAKRRR"
     gene            complement(2216..>2403)
                     /locus_tag="VFV30_05555"
     CDS             complement(2216..>2403)
                     /locus_tag="VFV30_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847588.1"
                     /translation="AHFLLRGEVGEGAADHAGADKGNLLASHGANSSFPECGIAAGIR
                     TTLPIPQGAGIRLTVLP"
BASE COUNT          393 a          866 c          773 g          371 t
ORIGIN      
        1 ccccggtcac agttgcgggg gcagcggggg aattgaaccc ccttccctct tagacccggt
       61 ttcccgggta acccgtgacg agccccgccc atagccgggg ccgggccggg gcgcaagttg
      121 ggccaattct taaccgctta aagctatgcc cggtccccgt cccggttcgg tacgccgccc
      181 acggcgcgct ggccgggacc gcccttcccg gtttacgcgc ggaggcgcgt aaccgaaccc
      241 gagagccgat gatccccgaa ttcgatgata cacgttagcg ccttgtcctt tgaatcccgg
      301 ccgcgcgatt ttgccgcacg gttcacccgc cggggtgcgc ggggcctggg cacgcgccgg
      361 gcgcgccgcc acctgggccg ccgggcgctg gtactgctgg cggcgatcgc ggtgccagcc
      421 gtggccgcgc cgggggaatg gcgcccgttc gacatcggca acgccggaag cgccgacctg
      481 gcgatggaac cgatgccgtt cgaacgcagc ggcgaaagct ttcccggatc ggcgttttat
      541 taccttgccc caaccgaggc cctgcccggc gagatcggct cgttctcgct gatcagcgaa
      601 gccgcgccga tcggcacgac cgacttttcg ggcggcccgg tcgcccgcgc gatccggttc
      661 gacctgtcgg gcgtggaccg gacccgcgcg ctcgattgcc tgaccgcggc ggtctattac
      721 gaggcgcgca gcgagcccga ccacggccag cgcgccgtgg cccaggtggt gctgaaccgg
      781 gtggcccatc cggcctatcc caacacggtc tgcggcgtgg tctatcaggg cagcgagcgc
      841 accaccgggt gccagttcag ctttacctgc gacggatcgt tggccaagcg gcccaacccc
      901 tatttctggg agcgctcgcg ccgggtggcc gagatggcgc tgtcaggcca tgtcgaaacc
      961 agcgtgggcc ttgcgaccca ttaccacacg atccagatct acccctattg ggcgcccagc
     1021 ctcaacttca tcaccacgat cggcgcgcac cggttctaca gcttcaaggg caaggccggc
     1081 caatcgggca ccttccgctt tgcctatgcc gggggcgagc cgatggccgc gccccacgcc
     1141 cgcaacccgg cggcggacaa ggcgcccgat ccggtgctcg atccgctggc gctgcagaaa
     1201 tcgtacgagg acggcttcaa ggcctctccc gccgggaccg ggctggtcca gcccggcgat
     1261 gtcggcaagg ctccggccaa acccgccccg cccccgcgct atgcccccga agtggaaaag
     1321 cgcggcggcg accgccagta cagcggcgag aagctgcccg gatcgaccgg cgtgcggccc
     1381 gagtacgaga attcgggcaa gtggatcggc cagccggggg gatagtttgt ttgcccgcct
     1441 gacggcggtg cgggaaagcc atccggcttt ccctgcctgg ccggcccgct cccccggccc
     1501 aaccacccgt taaaggtacc ctgtgggtgg ttggggcgcc ccgacaaaac gtcccccgga
     1561 cgttttgcga tctcgcggca cgggagcggg ccggccaggc caagccaatc gcggatgcga
     1621 atggcgcacc gacaacaaaa gtaaaccctt ggcaaccata gccccacacg aaggcttagc
     1681 gcgccccggc tagagcggcc ttccagaaaa caaaattcga gtacaggttg catgtccttg
     1741 cgctttgccg ctgcccgatc ttcggttttt cccagctatc tgccccgcgc catggcagcg
     1801 cggatcgccg cccgcgccgc caatgacaac ggcgacgggg acagccacgc cgatacgctg
     1861 cgcgccgcgc tgaagcactt tgccgcctat ggaatggccg cggccggcga agcgcgcgac
     1921 cgcgcccgca aggcccattt cgcgggggat cgcgacggat accgccactg gctggggatc
     1981 tgccgcgcgc tcgaccgccg catggcccag gccctggtcg ccaacctggc caagcgccgc
     2041 cgctagggcg gccatcccct ttcctgccgg accgagcctt cgggccgcca ccgcggcggg
     2101 cgtttccgca cgcccggcgc ccgcgcgccc ttgcatcttt tgctattgcg aactattatc
     2161 agcaaatagt tggtcgtacg accttttacc agcttgcaat cgccaaaaca gccgcctagg
     2221 gcaggaccgt tagccggata cctgcgccct gcgggatggg taaggtcgtc cgaatgccag
     2281 ccgcgatccc gcattcaggg aaggacgaat tcgcaccatg gctcgcaaga agattgccct
     2341 tatcggcgcc ggcatgatcg gcggcaccct cgcccacctc gccgcgaaga aggaaatggg
     2401 cga
//
LOCUS       DASUDV010000257         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_626839,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000257 DASUDV010000000
VERSION     DASUDV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626839"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VFV30_05560"
     CDS             complement(<1..1251)
                     /locus_tag="VFV30_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX4847589.1"
                     /translation="MPGLNNREAALAALAEGRPALVWQRLIADTETPVGAAVKLIEPG
                     RGDFLLESVQGGEVRGRYSLLGLDPDLVFRAQGTSAEINRAWRHDRSAFAPLPGDSLT
                     ELRALVEACKIDVPQPLPSALACLVGYFGYETYGLIEKLPRPAPNALALPDMLFVRPT
                     LLLVFDRLSDELFAIAPVWPGGGAPERMVELAGERIEAALARLTLPAPAPTRTTDLPL
                     PALEPVLARETYAEMVLKAKDYIAAGDIFQVVLAQRFTTPFALPALDLYRALRRINPS
                     PFLYFLDLPGFALTGSSPEILVRVRDGEVTIRPIAGTRPRGKTPEEDRENEVSLLADP
                     KERAEHLMLLDLGRNDVGRVAESGTVQVTESYTVERYSHVMHIVSNVVGKLDSARHDA
                     LDAMFAGFPAGTVSGAPKIRACEII"
     gene            complement(1264..3198)
                     /locus_tag="VFV30_05565"
     CDS             complement(1264..3198)
                     /locus_tag="VFV30_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX4847590.1"
                     /translation="MLQFFRKSFNSKFGLALVMGFLLLLGLAFAAGDIAGNQTFGGVA
                     GGDRAATVGKGRVDTSEVERAVTRTVENLRREQPTVTLKDFIAQGGFDQVLRNVIDLA
                     AIHEFGKKHGLFIGDRLIDSEIAKLPDVQGPDGKVSDQLYQAFLRQRGLTDAQLRREL
                     GNSLMARQLLANADLGVAVPSAVVQRYAAVVTERRTGQIGLLPSAAFAPKTVPSDSEL
                     GTWYAANQKTFVRPERRVIRFATFTDAVLKTVPAPTEAEIAAAYNANKAKYEASESRK
                     VSQLILPTEAAAKAALAETAGKPLEAVAAGKGLSVATLAPLGKSQLAIQTSQAAANAT
                     FDAPKGKVLGPIKAPLGWVLLRVDAIEGKAGKTLDQARAELLPELTAQKRRAALTDFS
                     ARIEEEFDNGSTLTDVAKELGLTLTETPPLLANGQVFGQDGKTAPAELARVFTTAFAM
                     ESEGQPQLAEVDPGKQFIVFDVAQIQPAAPPPLAEVKQQAIAEYQLAKGAIAARAAAL
                     KIEAQVKQGKDLGAAMAALGLPLPPVDQVNLPREQVQQMGQQTPPPLAMLFALAKGQV
                     KLMGAPRNRGWYVVKVTDVIPGQVAANDPRLPEFQQTMARVTSQEYSQQLRAAMRGEV
                     GAKRNDAAINALKTRLSGGN"
     gene            3415..>3642
                     /locus_tag="VFV30_05570"
     CDS             3415..>3642
                     /locus_tag="VFV30_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX4847591.1"
                     /translation="MANRPFIVGNWKMNGTRAMLAEARAIDRGAARHPGVRVAVAPPF
                     TLIQAMAENAETMAIGGQDCHAVASGAYTGDV"
BASE COUNT          578 a         1184 c         1276 g          604 t
ORIGIN      
        1 gatgatctcg caggcgcgga ttttcggcgc accgctgacc gtcccggcgg gaaagccggc
       61 gaacattgca tcaagggcat cgtgccgcgc gctgtcgagc ttgccaacga cgttcgagac
      121 gatgtgcatg acgtggctgt agcgctcgac cgtgtagctt tcggtgacct gcacggtgcc
      181 agactcggcc acccggccga catcgttgcg gcccagatcg agcagcatca ggtgctcggc
      241 gcgttccttg ggatcggcca gcaggctgac ctcgttctcg cggtcttcct cgggcgtctt
      301 gccgcgcggg cgggtgccgg cgatcgggcg gatcgtcact tcgccatcac ggacgcggac
      361 gaggatttcc gggctcgatc ccgtcagggc aaagcccggt aggtcgagga aatagagaaa
      421 cggcgaggga ttgatccgcc gcagcgcgcg gtagaggtcg agcgcgggga gggcgaaagg
      481 cgtggtgaaa cgctgcgcca gcacgacctg gaagatgtcc ccggcggcga tgtagtcctt
      541 cgccttcagc accatctcgg cataggtttc gcgggcaagg accggttcca gcgcaggcag
      601 gggcaggtcg gtggtgcggg tcggggcggg agcgggcaga gtcaggcggg ccagcgccgc
      661 ctcgatccgc tccccggcca gctcaaccat ccgttcgggc gcgccgccac cgggccagac
      721 cggggcgatg gcgaagagct cgtcgcttag ccggtcgaac accagcagca gggtcgggcg
      781 cacgaacagc atgtccggca gcgccagggc attgggcgcc gggcgcggca gcttttcgat
      841 caggccatag gtttcatagc cgaaatagcc gaccaggcag gccagcgccg aaggtagcgg
      901 ctgcggcacg tcgatcttgc aggcttcgac cagcgcgcgc agctcggtca ggctgtcacc
      961 gggcaggggc gcaaaggcgc tgcggtcatg ccgccaggcg cggttgatct cggccgaggt
     1021 accctgggcg cggaacacca ggtccggatc gagcccgagc aggctatagc gcccgcgcac
     1081 ttcgccgccc tggaccgatt ccaacaggaa atcgccgcgg cccggttcga tcagcttcac
     1141 cgccgcgccg accggagtct cggtatcggc gatcagccgc tgccagacga gcgcgggccg
     1201 cccctcggcc agcgccgcca gcgcggcttc gcggttgttc aggcccggca cgcgcgcgtc
     1261 ccgtcagttg ccgccggaca ggcgggtctt gagcgcgttg atcgcggcgt cgttgcgctt
     1321 ggcgcccact tcgccccgca tcgcggcgcg cagctgctgc gagtattcct gcgaagtgac
     1381 gcgcgccatg gtctgctgga attcgggcag gcgcggatcg ttggcggcga cctggccggg
     1441 gatgacgtcg gtcaccttga ccacgtacca accacggttg cgcggcgcgc ccatcagctt
     1501 gacctggccc ttggcaaggg caaacagcat ggccagcggc ggcggggtct gctggcccat
     1561 ctgctggacc tgctcacgcg gcaggttgac ctgatcgacc ggcggcagcg gcagaccaag
     1621 cgcggccatg gccgcaccca ggtccttgcc ctgcttgacc tgggcttcga tcttcagcgc
     1681 cgcggcgcgc gctgcgatcg cccccttggc cagctggtat tcggcaatcg cctgctgctt
     1741 gacctcggcc agtggtggcg gggcggcggg ctggatctgg gcgacatcga acacgatgaa
     1801 ctgcttgccg ggatcgacct cggcgagctg cggctggcct tcgctttcca tcgcaaaggc
     1861 ggtggtgaag acgcgggcca actcggccgg ggcggtcttg ccgtcctggc caaagacctg
     1921 gccattggcg agcagcggcg gcgtttcggt cagcgtcaga cccagttcct tggcaacatc
     1981 agtgagggtc gagccattgt cgaactcttc ctcgatccgg gccgagaagt cggtcagcgc
     2041 ggcacggcgc ttttgcgcag tcagttcggg caacagttcg gcgcgggcct ggtcaagcgt
     2101 cttgccggcc ttgccctcga tcgcgtcgac ccgcagcagc acccagccga gcggggcctt
     2161 gatcggaccc agcaccttgc ccttgggtgc atcgaaggtg gcattggctg cagcctgcga
     2221 ggtctggatc gccagctgcg acttgcccag cggcgccagc gtggcaacgc tgaggccctt
     2281 gccggcggca acggcttcca gcggcttgcc ggcggtttcg gccagtgcgg ccttggcggc
     2341 ggcttcggtc ggcaggatca gctggctgac cttgcggctt tcgctggctt catacttggc
     2401 cttgttggcg ttgtaggcgg cggcaatctc ggcctcggtc ggcgccggca cggtcttcag
     2461 cacggcgtcg gtgaaggtcg caaaacggat cacgcggcgt tcggggcgga caaaggtctt
     2521 ctggttggcc gcgtaccagg tgccgagttc gctgtcgctt gggacggttt tcggcgcgaa
     2581 tgcggccgaa ggcagcaggc cgatctggcc ggtgcggcgc tctgtcacca cggcggcata
     2641 gcgctgcacc acggccgagg ggacagcgac gccaaggtcg gcattggcaa ggagctggcg
     2701 ggccatcagc gaattgccca gctcgcgccg caactgcgcg tcggtcagcc cacgctggcg
     2761 caggaaggct tggtacagct ggtcgctgac cttgccatcg gggccctgga catcgggcag
     2821 cttggcgatt tcgctgtcga tcagccggtc accgatgaac agcccgtgct tcttgccgaa
     2881 ttcgtggatc gcggcgaggt cgatcacgtt gcgcagcacc tgatcgaagc cgccctgagc
     2941 gataaagtcc ttgagcgtga cggtcggctg ctcgcgccgc aggttctcga ctgtgcgggt
     3001 taccgcgcgt tcgacttcag acgtatcgac ccggcccttg ccgaccgtcg ccgcccggtc
     3061 acccccggcg acgccgccaa aggtctggtt tccggcgatg tcgcccgccg caaaggccag
     3121 gcccagcagc agcaggaagc ccatgaccag ggccaggccg aacttcgaat tgaaggactt
     3181 gcggaagaac tgcagcatgc caactcgcca gaattaagtg cttggcccgc gattgcagac
     3241 ccgaggcgcg gctttaggca gcatttgccg cgcgggcaat gccgcttggc cacggaacgg
     3301 acaaagcccg cgatgatcca cagcctgctt tgacaagcgg ccccccgctg gcctaggcgc
     3361 atggtttaag ccgggtgcga ccccgtgaaa gaacaagata gcaggacaat aggcatggct
     3421 aaccgaccct tcattgtcgg caactggaag atgaacggca cgcgcgcgat gctggccgaa
     3481 gcccgggcaa tcgatcgcgg cgcagcccgg catcccggcg tgcgcgttgc cgttgccccg
     3541 ccgttcacgc tgatccaggc gatggccgag aatgccgaaa ccatggcgat tggcgggcag
     3601 gattgccacg cggtcgccag tggggcctat accggcgatg tt
//
LOCUS       DASUDV010000258         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_958041,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000258 DASUDV010000000
VERSION     DASUDV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958041"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2034)
                     /locus_tag="VFV30_05575"
     CDS             complement(<1..2034)
                     /locus_tag="VFV30_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847592.1"
                     /translation="MDILGTSGNDILDGTSGNDLIVLGGGVDTVHADDGDDEIVIAAP
                     IGNGSVIDGGLGFDTMVIRPAPGYPSNAGPDTSGYIAAYPTSIYGVEAMRFESVANSQ
                     LYMAVLHPVVEGGLTTMVGGAGADILLDVVFAAGTYTMANYSLSNWTNDINDPNYDYV
                     SLYVAPGAPGDFTLIAREGLQSVQSLFAGAGNDTLIGSSGSELLTAGAGFDQLFGNGG
                     DDRLFADNLTAYGASPTVRSFSGVFDGGDGNDVLAVGGIVDLTGATISNIETLSLLGS
                     FAAPFPGGNGRAPASVTLNASQIAAGFGASPVLAGTGLVNVQLASGQSFNASAWQFSA
                     GSDITVNFIGTSGTETITGTTGDDVILPAGGVDTIHADAGNDEIVLTAPIGAGSILDG
                     GIGYDTLVLRPQSTYPNATGGTVTQYNAYFPTQIQGLEAVRFESNPGDFLSLVVLDIQ
                     RAGSGMTTFIGGAGRDMVYDIAHAAGTYTMPEVSFSNWNTTLNDPNSDFIVLFGSAPG
                     DYVLNAREGLGSNQGLIGNAGNDILNGSSGSDSLDGRGGVNQLYGNGGNDWLFAENRT
                     YFGEPTTTFTFAGNIFDGGSGDDLLLVGGMVNFQGTLSNLEGIYFQPSFGNSGPGTYG
                     IAPAHLILGASNAYAMPGNTRLGGVGTLQIDMGTATVLNLSGYQYSPGANVQVI"
     gene            complement(2160..>3483)
                     /locus_tag="VFV30_05580"
     CDS             complement(2160..>3483)
                     /locus_tag="VFV30_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4847593.1"
                     /translation="GSTEVAKLIQAELAGHTAPHGAPIPLIAETGGQNAMIVDSSALA
                     EQVVFDVIASAFDSAGQRCSALRVLCLQDDVADRQLAMLKGALAELALGRPDRLSVDI
                     GPVISAEARWGIEDHVARMRAAGCRVEQLPLPAETARGTFVAPTIIEIESIAQLSREV
                     FGPVLHVLRWKRDELDALIDAINATGYGLTFGLHTRLDEVVERVTARAGAGNVYVNRN
                     VIGAVVGVQPFGGRGLSGTGPKAGGPLYLGRLVQSAPQTGLAGQAPDPVLGELAGWLE
                     QHGEPELAQRARSYRALNGHAAELPGPVGERNLYALHPRGRVLALPNSREGLLAQLVA
                     ALGEGNRVAVVWPGITLDTLPAQVAQRIDWLPALPERSDFAAVLIEPRSGGIAELLAR
                     VAAMDGPIPLVQMADGAGHYRADWLVEEVSVSINTTAAGGNASLMALV"
BASE COUNT          590 a         1124 c         1179 g          590 t
ORIGIN      
        1 gatcacctgg acattcgcgc cgggcgaata ctggtagccc gacaggttga ggacggtggc
       61 cgtgcccatg tcgatctgga gcgtgccgac cccgcccagt cgggtgttgc ccggcatggc
      121 ataggcattg ctcgcgccaa ggatgaggtg ggccggtgcg atgccatagg tgcccggacc
      181 tgaattgccg aaacttggct ggaaatatat gccttcaagg ttggacagcg taccctggaa
      241 gttgaccatg ccgccaacca gcagcagatc atcgcccgag ccgccatcga agatgttgcc
      301 ggcaaaggtg aaggtggtgg tcggctcgcc aaagtaggtg cggttttcgg caaacagcca
      361 gtcattgccg ccgttgccgt aaagctggtt taccccgccg cgcccgtcga gcgagtccga
      421 tccgctcgat ccgttgagga tatcgttgcc cgcgttgccg atcaggccct gattgctgcc
      481 aagtccttcg cgggcattga gcacatagtc acccggcgcg ctgccaaaca gcacgatgaa
      541 atcgctgttc ggatcgttca gggtggtgtt ccagttgctg aacgacacct cgggcatggt
      601 gtaggtaccg gcagcgtggg cgatatcgta gaccatgtcg cggccagcgc cgccgatgaa
      661 ggtggtcatt cccgatccgg cgcgttggat gtcgagcacg accaggctca ggaagtcgcc
      721 cggattggat tcgaaccgca ctgcctcgag gccctggatc tgggtcggga aataggcgtt
      781 gtactgggtg acggttcccc ccgtggcgtt gggataggtg ctctgcggac gcaggacgag
      841 cgtatcgtag ccgatcccgc catcaaggat cgatcccgca ccgatcggcg cggtcagcac
      901 gatttcgtcg ttcccggcat cggcgtggat cgtgtcgacc ccgccggccg gcaggatcac
      961 atcgtcgccg gtggtgccgg tgatggtctc ggtgccgctg gtgccgatga agttgaccgt
     1021 gatgtcgctt cccgcggaga actgccaggc cgaggcattg aagctttgcc cgctggcaag
     1081 ctgcacgttg accaatccgg tcccggccag aaccgggctg gcgccgaacc cggcggcgat
     1141 ctggctggcg ttcagagtca cgctggccgg cgcacggcca ttgccgccgg ggaagggggc
     1201 ggcgaacgat ccgagcagcg agagcgtttc gatgttgctg atcgtcgccc cggtcaggtc
     1261 cacgatcccg ccaactgcca gaacgtcgtt gccatcaccg ccatcgaaaa caccggaaaa
     1321 gctgcgcacg gtcgggctgg cgccgtaagc ggtcagatta tcggcaaaca agcggtcatc
     1381 accaccattg ccgaacaact ggtcgaatcc tgcaccggcg gtgagcagtt cggaaccgct
     1441 cgagccgatc agggtgtcgt tgccggcccc ggcgaagagg ctctggacgc tctgcagccc
     1501 ttcgcgcgcg atgagcgtga aatcaccggg cgcgcccggt gcgacataga gagagacgta
     1561 gtcgtagttg ggatcgttga tgtcgttggt ccagttgctc aggctgtaat tggccattgt
     1621 gtaggtgcct gcggcgaaca ccacgtcgag caggatgtcg gccccggcgc cgcccaccat
     1681 ggtggtcagt ccgccttcga ccaccgggtg gagcaccgcc atgtagagct gggaattggc
     1741 caccgattca aaccgcatgg cttcaacccc gtagatgctg gtggggtagg cggcgatata
     1801 gcccgaggtg tccggacctg cgttcgaggg gtagcctggc gctggcctga tcaccatcgt
     1861 gtcgaagccc agtccgccgt cgatcacgga cccgttgccg atcggggcgg cgatcacgat
     1921 ctcgtcatcg ccgtcatcgg cgtggaccgt gtccaccccg ccgcccagga cgatcagatc
     1981 gttgcccgag gtgccatcga ggatgtcgtt gccgctggtg ccaagaatgt ccatgcccgc
     2041 ctccaattgg tgtttggaag cgatgctagg actcggcgtg gctgcgcgat gtgacaaacg
     2101 ccacaaggct tcggccggag ccgcgaaaat gtcccggatc gggactttgc ggccagaccc
     2161 tagactagcg ccataaggct ggcattgccg ccggcagcgg tggtgttgat cgagaccgac
     2221 acttcctcga ccagccagtc ggcccggtaa tggccggcgc catcggccat ctgcaccagc
     2281 gggattggcc cgtccatcgc cgcgacccgg gcgagcagtt cggcaatgcc gccagagcgc
     2341 ggttcgatga gcacggcggc gaagtcgctg cgctcgggca gggcgggcag ccagtcgatc
     2401 cgctgggcca cttgtgcggg cagggtgtcg agggtgatcc ccggccagac caccgccacc
     2461 cggttgcctt cgccaagcgc ggcgaccagc tgggcgagta ggccctcgcg gctgttgggc
     2521 agggcgagca cgcggccgcg gggatgaagg gcatagagat tgcgctcgcc caccggcccg
     2581 ggcagttccg cggcatggcc gttgagcgcg cggtagctgc gggcgcgctg ggccagctcg
     2641 ggttcgccgt gctgttcaag ccagccggcc aattcgccca gcacgggatc gggcgcctgg
     2701 ccagccagcc cggtttgggg cgccgactgc accagccggc ccagatagag cggcccgccg
     2761 gccttgggcc cggtgcccga caggccgcgc ccgccaaacg gctgaacgcc caccaccgcg
     2821 ccgatcacgt tgcgattgac gtagacgttg cctgcgcccg cccgcgcggt gacgcgttcg
     2881 accacctcgt ccagccgggt gtgcaggccg aaggtcaggc catagccggt ggcattgatc
     2941 gcatcgatca gcgcgtcgag ctcgtcgcgc ttccagcgca gcacgtgcag caccgggccg
     3001 aacacctcgc ggctcagctg ggcgatgctc tcgatctcga tgatcgtcgg ggcaacgaag
     3061 gtgccgcgcg cggtttcagc gggcaggggc agctgctcta cccggcagcc cgcggcccgc
     3121 atccgcgcga catgatcctc gatcccccaa cgcgcctcgg cactgatcac cgggccgata
     3181 tcgaccgaaa ggcgatcggg ccggcccagc gcaagttcgg ccagcgcgcc cttgagcatg
     3241 gccagctgac ggtcggccac atcgtcctgc aggcacagca cgcgcagggc cgagcagcgc
     3301 tgcccggcac tatcgaaggc cgatgcgatc acgtcgaaaa ccacctgttc cgccagagcc
     3361 gaactgtcga cgatcatggc gttctggccg ccggtttcgg caatcagcgg gatcggcgcg
     3421 ccgtgcggtg cggtgtgacc ggcgagttcc gcctggatca gcttcgcaac ctcggtcgat
     3481 ccg
//
LOCUS       DASUDV010000259         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1731639,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000259 DASUDV010000000
VERSION     DASUDV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731639"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFV30_05585"
     CDS             <1..298
                     /locus_tag="VFV30_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847594.1"
                     /translation="YAYNTYAVASKIMAGGKIGAEASLNKIFWSELDRAMHRTAMQLL
                     GAHAELKRFGNGQVNQWLEGYIFSLSGPIYAGSNEVQRNIIAERLLGLPRVGAG"
     gene            298..1242
                     /locus_tag="VFV30_05590"
     CDS             298..1242
                     /locus_tag="VFV30_05590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847595.1"
                     /translation="MDFRFSDDQLTLVDSVRDYLAGTHGPEVLRRLDAESNRDPAIWQ
                     GLVEMGLTALCVPEEHGGLGMGLLEAVVIAAECGRACLAEPLVDTAFVAVPWLVAKGE
                     TADLAAIVAGERKIALPHVLNPWVADGAEDFGDKPLQSVDPLRNLVRFPATGSTDDHL
                     LNLGALMSAAQLLGLADAMLHQAVEYAKVRTQFGQPVGAFQGLKHQLSSCAVAIEFAK
                     PVVWRAAQALQDGAASAALHVSHAKLAATDAAIQTAETAIQVHGAMGYTYEVDLHFWM
                     KRAWALAGAWGDRSFHYKRVDKAVLSGAHPIGPEHTFA"
     gene            1251..>2235
                     /locus_tag="VFV30_05595"
     CDS             1251..>2235
                     /locus_tag="VFV30_05595"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4847596.1"
                     /translation="MPEAYIIDAVRTPIGRKKGSLAHLHPADLGAHPIKALVERTGID
                     ANAVDDVVWGCCDTIGPQAGDIGRTVWLVAGLPLHVPGTTIDRQCGSSQQAVHFAAQG
                     VMSGTQDLVVAGGSQAMNAVPISAAMFAGQPYGFGDPFTGSKGWVERFGDGLLNQINS
                     AEMIAAKWQISREAMEAFALASHQRAQAASEGGLFANEIAPLEGLGADETIRPGTTLE
                     GLASLKTVQEGGVITAGVASQNCDGAAALLIASEQAVKDHGLKPRARIHHISVRADDP
                     VWMLTAPIPATRHALKKAGMSVGDIDLFECNEAFASVPMAWMKELGIPHEKV"
BASE COUNT          402 a          702 c          749 g          382 t
ORIGIN      
        1 ctatgcctac aacacctacg cggtcgcctc gaagatcatg gccgggggca agattggggc
       61 cgaagccagc ctcaacaaga tcttctggtc cgaacttgac cgggcgatgc accgcaccgc
      121 gatgcagttg ctgggcgccc atgccgaact caagcggttt ggcaacgggc aggtgaacca
      181 gtggctggaa ggctatatct tctcgctttc gggtccgatc tacgccggtt cgaacgaggt
      241 tcagcgcaac atcatcgccg agcggctgct gggcttgcca agggtaggag cgggctgatg
      301 gacttccgtt tctcagacga tcagctgacc ctggtagaca gcgtgcgcga ctacctggcc
      361 ggaacacacg ggcccgaagt gctgcgccgg ctcgacgccg aaagcaaccg cgatccggcg
      421 atctggcagg gcctcgtcga gatgggcctg accgccctgt gcgtacccga agagcatggt
      481 ggtcttggta tggggctgct cgaagctgta gtgatcgccg ccgagtgtgg ccgcgcctgt
      541 ttggccgagc cgctggtcga taccgccttc gtcgccgtgc cctggctggt ggcgaagggc
      601 gaaaccgccg atcttgcggc aatcgtcgct ggtgagcgca agatcgcctt gccgcacgtg
      661 ctcaatccct gggtggctga cggcgccgag gactttgggg acaagccgct gcaaagcgtc
      721 gatccgctgc gcaatctggt ccgcttcccg gcgacgggca gcaccgatga tcacctgctc
      781 aacctcggcg cgctgatgag cgcggcccag ctcttggggc tggccgatgc catgcttcac
      841 caggcggtcg aatatgccaa ggtccgcacc cagttcggcc agccggtcgg cgcgttccag
      901 gggttgaagc accagctttc cagctgtgcg gtggcgatcg agtttgccaa accggtggtc
      961 tggcgcgcgg cgcaggcgct gcaggatggc gctgccagcg cggcgctgca tgtcagccac
     1021 gccaagctcg cggcaaccga tgccgcgatc cagaccgccg agaccgcgat ccaggtccat
     1081 ggggcgatgg gctataccta tgaagtcgat ctgcacttct ggatgaagcg cgcctgggct
     1141 ctggccgggg cctggggcga tcgttctttc cactacaagc gggttgataa agccgtgctg
     1201 agcggtgccc atccgatcgg ccccgaacac acctttgcgt gagaaaacaa atgcctgaag
     1261 cctatatcat cgacgccgtc agaaccccga tcgggcgcaa gaagggcagc cttgcccatc
     1321 ttcacccggc agaccttggc gcgcatccga tcaaggcgct ggtcgagcgc accgggattg
     1381 acgccaatgc ggtggatgat gtggtctggg gctgctgtga taccatcggc ccgcaggccg
     1441 gtgacatcgg ccgcaccgta tggctggttg cgggactgcc gcttcacgtg cccggcacca
     1501 cgatcgaccg gcaatgcgga tcaagccagc aggcggtgca ctttgcagca cagggcgtga
     1561 tgagcggcac ccaggatctg gtcgtggcgg gcggcagcca ggcgatgaac gcggttccca
     1621 tctcggccgc gatgtttgcg gggcagccct atggctttgg cgatccgttc accggatcga
     1681 agggctgggt cgagcggttt ggcgatggcc tgcttaacca gatcaattcg gccgaaatga
     1741 tcgccgccaa gtggcagatc agccgcgagg cgatggaagc gttcgcgctg gccagccacc
     1801 agcgtgccca ggccgcttcc gaaggcgggt tgtttgccaa cgaaatcgcc ccgctcgagg
     1861 ggctgggcgc ggacgaaacg atccgtcccg gcaccacgct cgaagggctc gccagcctca
     1921 agaccgtgca ggaaggcggc gtgatcaccg ccggggtcgc cagtcagaac tgcgatggcg
     1981 ccgctgcctt gctgatcgcc agcgaacagg cggtgaagga ccacgggctg aaaccgcgtg
     2041 cccgcatcca ccacatttcg gtgcgggccg acgatcccgt ctggatgctc accgcgccga
     2101 tccccgccac ccggcacgcg ctgaagaagg ccggaatgag cgtgggcgac atcgacctgt
     2161 tcgaatgcaa cgaagccttt gcatcagtgc cgatggcctg gatgaaggag ctgggcattc
     2221 cgcacgagaa ggtca
//
LOCUS       DASUDV010000260         6675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1444113,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000260 DASUDV010000000
VERSION     DASUDV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444113"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VFV30_05600"
     CDS             complement(<1..1488)
                     /locus_tag="VFV30_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4847597.1"
                     /translation="MKAAAAASGALLPERLALGAALLASLVLVWFATRDALVMAVFAA
                     GLVGLGALAWFLTRKAPTAAEAEYALPDWSVTVAAIDRSDAAIAVTDRAGRLVCANPL
                     YEKWFTAGHAPPRLPVDNTSLERLAKAGRAAWRDGSAAADLLESAEGGRWKAQALRAG
                     RGDDYLVWTITALTPPDLVGELVPHLDGKLGRALSQAGFAVAIVDPDGKIRAASSGFA
                     QRATGDAAAILTGQEFTSLLRQDDRDTITWAREGRRGAPLSLFYLPVADPDAVASVDY
                     DNTPSLILIADHGVGIGGGGSEATAAVPHLEALLAQLPLGLAMADRDGRLLFANVAFM
                     RAAGREGEAPPTYPTDLVVQEDKGPISDAIRRFAQGPASSGEIGVRLRSQPEEPVTLS
                     LAGVRGLGEAAVLLGLTDNTEETRLKRQVAQATKMQAVGQLAGGVAHDFNNVLTAILG
                     TCDLMLMRHTPGDSDYDDIQQIRANSNRAASLTRQLLAFSRQQTLR"
     gene            complement(1485..2051)
                     /locus_tag="VFV30_05605"
     CDS             complement(1485..2051)
                     /locus_tag="VFV30_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX4847598.1"
                     /translation="MSKLVSRITAWSHRNMPKREELEHNRWVGPFARRPELWRFTRRS
                     VPRGVAVGLLVGIFALIPGIQIVGAALMCVPSRGNIPLAALMTFLSNPATTPFIIAAS
                     IFLGNKLGFHADVTTFYALMNKGAGMREWAYWLLSDAAPAMLLGLFVISVITASIGYL
                     VSSFTWTWWIRHKRKVALERIRETRGEG"
     gene            complement(2048..2530)
                     /gene="smpB"
                     /locus_tag="VFV30_05610"
     CDS             complement(2048..2530)
                     /gene="smpB"
                     /locus_tag="VFV30_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX4847599.1"
                     /translation="MARPTHAQFDKKKIVAENRRARFDYFIEDTTEAGIALTGTEVKS
                     LRFGEGSIAESYAEVKNGEVWLINSNVPEFSHGNRFNHEPKRPRKLLLKAREIDRFTG
                     AVERKGMTLVPLSIYFNGRGRAKVELALAKGKNVADKRATIKERDWKRDQQRIMRDYK
                     "
     gene            complement(2642..3031)
                     /locus_tag="VFV30_05615"
     CDS             complement(2642..3031)
                     /locus_tag="VFV30_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX4847600.1"
                     /translation="MAARSADGGVIVRNPPAHDYAKARRLRGSMSLPEVLLWEYLRKK
                     PNGIKFRRQHPVGNYVIDFYCPLKRIGIEIDGIAHEMGDRPLKDEQRDRWLADQHIRM
                     MRIPASEVLRSVADAATAIVALCGDPD"
     gene            complement(3063..3944)
                     /gene="dapA"
                     /locus_tag="VFV30_05620"
     CDS             complement(3063..3944)
                     /gene="dapA"
                     /locus_tag="VFV30_05620"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX4847601.1"
                     /translation="MFSGSIPALVTPFKDGAVDEAAFRRLIDWQIAEGSGALVPCGTT
                     GEAPTLSNEEQHRLFAICVEQAAGRVPVIAGCGSNDTQTALWHMREAKAVGAAAALMV
                     APYYNRPSQAGLLAHFSYLAEAVDLPIILYNVPGRTVTDIKPETTIELARRFPDRIVG
                     IKDASGDMTRLTAHRLALGAGFVQLCGNDDMALHYNAGGGSGCISVTANIAPRLCAEF
                     QAASLAGDTARAHALNEKLFPLHQALFTDASPGPIKYAMAKVLGWIGEEVRLPLVSAS
                     AESRKAVDAGLAHAGLT"
     gene            4010..6007
                     /locus_tag="VFV30_05625"
     CDS             4010..6007
                     /locus_tag="VFV30_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4847602.1"
                     /translation="MSSMLRTSMISALAMLALLPAPALAQDGALDSGGKDEEAVQWDQ
                     ARTALRALPPGDMVQAVDRWKMLVASDRFGFGDYAGFLLAYPGLPEETKLRGFAERAL
                     DREAVPPAQVAAFFDRFPPQSNAARAHYAMALIALGRPDANRVALEAWRGGAMSEANE
                     AALLARLGPGLTLADHDSRMDALLWAGSSAAATRQLAYVSPERRMRFSARLGLVNGQD
                     TVAAGYDAGPDALSDPGYVFNRSRMLRTGGQPGAAAAMLATRPRLNSLPREPRRWIGE
                     LLAVARKADASSAVRIAASIDDTFPPGTDVSQQSFAIRDDYTSLMWLGGTRALWDMGD
                     GARAAPLFYRYGAAARSPQTRSKGFYWAGRSAAQAGNQAEANRYFEMAAIYPDQYYGM
                     LALERLGRPLPSLMTAPRAVPTPAERQAFNDRPLTRAVREVARESDWPTSVRFFKEIA
                     EQAVTESDHVLVADLARETGRRDLAVILGQAAHSDGHGVFRQTSFPLIPTPAGSNWTF
                     VHAITRQESQFSMNAISHAGARGLMQLMPGTAREQAGKMGFEYDPQALTRDAGYNLTL
                     GDGYFRRMLDYYGGSYPLAVAAYNAGPGNVNKWLRLNGDPRNGGIDWVEWVEKIPVSE
                     TRNYVQRVLENAVVYEALNPKRAIQRGPNPLSQFLGKRYPG"
     gene            6154..6648
                     /locus_tag="VFV30_05630"
     CDS             6154..6648
                     /locus_tag="VFV30_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX4847603.1"
                     /translation="MSIVRDGPPITPAGMAALRARYDHLLGTERPAIVEIVSWAAGNG
                     DRSENGDYLYGRKRMREIDRELAHLARRMKNCRVIDPARQEDQSRAWFGAVVTIADED
                     DNQRTVTLVGDDEQDASAGLIGWSAPMARALRGAAVGDLRKVHLPGGEKEWEVIAIRY
                     PESA"
BASE COUNT         1077 a         2262 c         2258 g         1078 t
ORIGIN      
        1 ccgcaatgtc tgctgccggg aaaacgccag caactgccgg gtcagcgagg cggcgcggtt
       61 ggagttggcg cgaatctgct gaatgtcgtc gtaatccgaa tcgccgggcg tgtgccgcat
      121 cagcatgaga tcgcaggtgc cgaggatcgc ggtcaacacg ttgttgaaat cgtgcgccac
      181 cccgccggcc agctggccga ccgcctgcat cttggtggcc tgggcaacct gccgcttgag
      241 ccgggtttcc tcggtgttgt cggtcaggcc cagcaagacc gccgcctcgc ccagtccgcg
      301 caccccggca aggctcagcg tcaccggctc ttccggctgg ctgcgcagcc gcacgccgat
      361 ttcgccgctg ctggcgggac cttgcgcaaa gcggcggatc gcgtcggaga tcgggccctt
      421 gtcttcctgc acgaccagat cggtcggata ggtcggcggt gcctcccctt cccgcccggc
      481 ggcgcgcatg aaggccacgt tggcgaacag cagccgccca tcgcggtcgg ccatggcgag
      541 gcccagcggc aattgcgcca gcagcgcctc caggtgcggc accgcggcgg ttgcctcgct
      601 cccgccgccg ccgatcccca cgccgtgatc ggcgatcagg atcagcgagg gggtgttgtc
      661 gtaatcgacc gaagccaccg catcgggatc ggccaccggc aggtagaaca ggctgagcgg
      721 cgcgccgcgg cgcccttcgc gggcccaggt gatggtatcg cggtcatcct gccgcagcag
      781 cgaggtaaac tcctgaccgg tcaggatcgc cgccgcatcg cccgttgcgc gctgggcaaa
      841 gccgctgctg gcggcgcgga tcttgccatc gggatcgacg atcgcgacgg caaagccggc
      901 ctgggacagc gcccggccga gcttgccatc aagatgcgga accagttcgc cgaccagatc
      961 cggcggggtc agcgccgtga tcgtccagac caggtaatcg tccccgcgcc ctgcgcgcag
     1021 ggcctgcgct ttccaccggc cgccctcggc gctttcgagg aggtccgccg cggcgctccc
     1081 atcgcgccag gcagcgcggc cggccttggc cagccgttcc aggctggtgt tgtcgaccgg
     1141 caagcgcggc ggagcatggc cggcggtgaa ccacttttcg tagagcgggt tggcgcagac
     1201 caggcgcccg gcccggtccg tcaccgcgat cgcggcgtcg ctgcggtcaa tcgcggcgac
     1261 cgtgaccgac cagtccggca gggcgtattc cgcctcggcg gcagtggggg ccttgcgcgt
     1321 caggaaccag gccagcgcgc ccaggccgac gaggccggcg gcaaagaccg ccatgaccag
     1381 tgcgtcgcgc gtcgcgaacc acaccagcac caggctggcg agcagcgccg ctcccagcgc
     1441 cagccgctcg ggcagcagcg cgccggaggc ggcggcggcc gccttcatcc ctcgccccgc
     1501 gtttcgcgga tccgctccag cgcaaccttg cgcttgtggc ggatccacca ggtccaggtg
     1561 aaggagctga ccagatagcc gatcgaggcg gtgatcaccg aaattacgaa cagccccagc
     1621 agcatcgccg gggcggcatc ggacagcagc caataggccc attcccgcat gccggcgccc
     1681 ttgttcatca gcgcatagaa ggtggtgaca tcggcatgga agcccagctt gttgccgagg
     1741 aagatcgagg cggcgatgat gaacggcgtg gtggccgggt tcgacaggaa ggtcatcagc
     1801 gcggcgagcg gaatgttgcc gcggctgggc acgcacatca gcgctgcgcc gacgatctgg
     1861 atccccggga tcagcgcaaa gattccgacc agcagaccga ccgcgacccc gcgcggcacc
     1921 gagcggcggg taaagcgcca cagctccggc cggcgcgcaa aggggccgac ccagcggttg
     1981 tgctcaagct cctcgcgctt gggcatgttg cggtgggacc aggcagtaat gcgggaaacc
     2041 agcttgctca tttgtaatcc cgcataatcc gctgctggtc tcgcttccag tcgcgttcct
     2101 tgatcgtggc gcgcttgtcc gcaacgttct tgcccttggc gagcgccaat tccacttttg
     2161 cgcggccgcg gccgttgaaa tagatcgaaa gcggcaccag cgtcatgccc ttgcgctcga
     2221 ccgctccggt gaagcggtca atctcgcgcg ctttcagcag cagcttgcgc gggcgcttcg
     2281 gctcgtggtt gaagcggttg ccgtggctga attcgggcac gttcgaattg atcagccaga
     2341 cctcgccgtt cttcacctcg gcataggact ccgcgatcga gccttcgccg aaccgcaggc
     2401 tcttcacctc ggtccccgtc agcgcgatcc cggcctcggt cgtatcctcg ataaaatagt
     2461 caaaccgcgc ccgccggttc tcggcgacga tcttcttctt gtcgaactgg gcgtgggtcg
     2521 ggcgggccat gatcgggggg catgtagggt gtgtgggagg aaaagggaac ggggtgagag
     2581 aaaatcctcc ctgtttgcgg agcgaatggg gaggtggcag cgcgcagcgc tgacggaggg
     2641 gtcaatcggg gtcgccgcaa agcgcgacaa ttgccgttgc tgcatcggca acagatctga
     2701 gcacctcgct ggccggaatc cgcatcatgc ggatatgctg atcagcaagc catctgtccc
     2761 gttgctcgtc cttcagagga cgatcaccca tttcgtgggc gatcccgtca atctcgatcc
     2821 cgatccgttt caatggacag tagaaatcga tgacatagtt accgaccgga tgttggcggc
     2881 gaaacttgat gccattcggc tttttgcgga ggtattccca gagtagaact tcgggcaaac
     2941 tcattgatcc acgcaacctg cgcgccttgg catagtcatg ggcgggtgga ttgcgcacaa
     3001 tcacccctcc gtcagcgctt cgcgctgcca cctccccatc gcttcgcgac agggaggatt
     3061 tttcatgtca gccccgcatg cgccagtccg gcgtccaccg ccttgcggct ttcggcgctg
     3121 gcactcacca gcggcaggcg gacctcttcg ccgatccagc ccagcacctt ggccatggcg
     3181 tacttgatcg ggccggggct ggcgtcggtg aacagcgcct ggtgcagcgg gaacagcttc
     3241 tcgttgagcg cgtgggccct ggccgtatcg ccggccaggc tggcggcctg aaactccgcg
     3301 cagagccgcg gcgcgatgtt ggcggtgacc gagatgcagc ccgatccgcc cccggcgttg
     3361 tagtgcagcg ccatatcgtc gttgccgcac agctgaacga agcccgcgcc cagcgccagg
     3421 cggtgcgcgg tcagccgggt catgtcgccg ctggcgtcct tgatcccgac gatccggtcc
     3481 ggaaagcggc gcgccagttc gatcgtggtt tccggcttga tgtccgtcac cgtgcggccg
     3541 ggcacgttgt aaaggatgat cggcaggtcg accgcttcgg ccagatagct gaaatgggcc
     3601 agcaacccgg cctggctggg ccggttgtaa tagggcgcga ccatcagcgc ggcggccgcg
     3661 ccgaccgcct tggcctcgcg catgtgccac agcgcggtct gggtgtcatt gctgccgcag
     3721 ccggcgatca ccgggacccg gcccgcggcc tgctccacgc agatcgcgaa cagtcggtgc
     3781 tgttcctcat tgctcagcgt cggcgcttcg ccagtcgtgc cgcagggaac cagcgcaccg
     3841 gagccttcgg caatttgcca atcgatcagg cggcgaaagg ccgcttcgtc caccgctccg
     3901 tccttgaacg gggtgaccag agccggaatt gagccggaga acatggactt taaccctgct
     3961 aatgcgccat acggcctgtt cagcctgcca taaggagcct tcccccagta tgtccagcat
     4021 gcttcgcacc tcgatgattt ccgcgcttgc catgctggcc ctgcttcccg cccccgccct
     4081 tgcccaggac ggagcgttgg acagcggcgg caaggacgag gaagccgtgc agtgggatca
     4141 ggcccgcacc gcgctgcgcg cgctgccgcc gggcgacatg gtccaggcgg tcgatcgctg
     4201 gaagatgctg gtggcaagcg accggttcgg ctttggcgat tacgccgggt tcctgctggc
     4261 ctatccgggc ctgccggaag agaccaagct gcgcggcttt gccgaacgcg cgctggaccg
     4321 cgaggcggtg ccgccggcgc aggtcgccgc cttctttgac cggtttcccc cgcaatcgaa
     4381 cgcggcgcgg gcccactatg ccatggcgct gatcgcgctg ggccggcccg atgccaaccg
     4441 cgtcgcgctg gaggcctggc gcggcggagc gatgagcgag gccaacgagg ccgcgctgct
     4501 cgctcggctg ggccccggcc tgaccctggc cgaccatgac agccggatgg acgcgctgct
     4561 gtgggccgga tcaagcgccg cggcgacccg ccagctggcc tatgtctcgc ccgaacggcg
     4621 gatgcgcttt tccgcgcggc tggggttggt caacgggcag gacacggtcg ccgccggata
     4681 cgatgccggg ccggacgcgc tgagcgatcc cggttatgtc ttcaaccgct cgcggatgct
     4741 gcgcaccggc gggcagccgg gcgcggccgc ggccatgctc gccacccgcc cgcggctcaa
     4801 ttccctgccg cgcgagccgc gccgctggat cggcgaactg ctcgccgtgg cgcgcaaggc
     4861 cgatgcgagc agcgcggtgc ggatcgccgc ctcgatcgac gataccttcc cgccgggcac
     4921 cgacgtcagc cagcaatcct tcgcgatccg cgacgattac acctcgctga tgtggctggg
     4981 cggaacccgc gcgctttggg atatgggcga cggcgcccgc gcggcaccgc tgttctaccg
     5041 ctatggcgcg gcggcgcgct ctccgcagac ccgctccaag gggttctact gggccggccg
     5101 ttccgccgcc caggccggca accaggcaga ggcgaaccgc tatttcgaaa tggccgcgat
     5161 ctatccggac cagtattacg gcatgctagc gctggagcgg ctgggccggc cgctgccctc
     5221 gctgatgacc gcgccgcgcg ccgttcccac cccggccgag cgccaggctt tcaacgaccg
     5281 tccgctgacc cgcgccgtgc gggaagtcgc gcgggaaagc gattggccca cctcggtccg
     5341 gttcttcaag gaaatcgccg aacaggccgt gaccgaatcc gatcacgtgc tggtggccga
     5401 tctggcgcgg gaaaccgggc ggcgcgatct ggccgtcatc ctgggccagg ccgcccattc
     5461 cgatggccac ggggtgttcc gccagaccag ctttcccctg atcccgaccc cggccggttc
     5521 gaactggacc ttcgtccacg cgatcacccg gcaggaaagc cagttctcga tgaacgcgat
     5581 cagccacgcc ggggcgcgcg gcctgatgca gctgatgccg ggcacggcgc gcgagcaggc
     5641 cggcaagatg ggctttgagt acgatcccca ggccctgacc cgcgatgccg gttacaacct
     5701 gaccctgggc gacggctatt tccgccgcat gctggattat tacggcggat cctatccgct
     5761 ggccgtcgcc gcctacaacg cggggcccgg caacgtgaac aagtggctgc ggctgaacgg
     5821 cgatccgcgc aacggcggga tcgactgggt cgaatgggtg gagaagatcc cggtatccga
     5881 aacccgcaac tatgtgcagc gggtgctgga aaacgcggtg gtctatgaag cgctgaaccc
     5941 caagcgggcg atccagcgcg ggcccaatcc gctcagccag tttctgggca agcggtatcc
     6001 tggctaacgc gctgctcagc aagcgcacct cggactcccc tcccgcttgc gggaggggcc
     6061 gggggagggc atgttgacac cggataagaa caccccctcc cctaacccct cccgcaggcg
     6121 ggaggggaaa ttgaggaatg gcgactgcgt gccatgtcga ttgtaagaga cggtccgccg
     6181 atcactcccg ccggtatggc ggcgctgcgc gcgcgctacg atcacttgct gggcaccgag
     6241 cgtccggcaa tcgtggagat cgtcagctgg gccgcgggca acggcgaccg cagcgagaac
     6301 ggcgactacc tctatggccg caagcggatg cgcgagattg accgcgagct ggcgcatctg
     6361 gcgcggcgca tgaagaattg ccgggtgatc gatccggccc ggcaggagga ccaaagccgg
     6421 gcctggttcg gcgcggtggt gaccattgcc gacgaggacg acaaccagcg caccgtcacg
     6481 ctggtcggcg atgacgagca ggacgcctcg gccgggctta tcggctggtc cgccccgatg
     6541 gcccgggccc tgcgcggcgc ggcggtgggg gatctgcgca aggtccacct gccaggcggc
     6601 gagaaggaat gggaagtgat cgcgatccgc tatccggaga gtgcctgagc atggccgaag
     6661 tcatcaacct gcgcc
//
LOCUS       DASUDV010000261         9031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_362069,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000261 DASUDV010000000
VERSION     DASUDV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362069"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..2287
                     /locus_tag="VFV30_05635"
     CDS             41..2287
                     /locus_tag="VFV30_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEX4847604.1"
                     /translation="MILAVVLSGATAARGEATKASPLLFPAPAKVEPEAGAFALAGAQ
                     VHVDDAGGRAAAARLAELLRRSGKPAPALAAKGKIRFVRDPSIASAEGYRLTVRPEAV
                     TLAASGDAGLFYGAQALWQLMVESRGGTIGAVTITDAPKYAWRGTMLDSVRHMQSPAF
                     IKQVIDRMALVGLNRFHWHLTDDQGWRFPVPGYPRLTQVAAWRQQAGAAGFDPQTGQP
                     VRYGGYYTPAQIREIVAYARARHIEVVPEVDFPGHATAAIAAYPGLASNFAPPAAPGA
                     DWGVYHNIIKPDEQGLKFARAVLDEVMRLFPGKHIHVGGDEAVKDYWKANPAIQAQIR
                     ALGLKDENALQGWFMAQLGAHVGRQGRRIVGWDEVLEGEVPGDAVVMSWRGASGALVA
                     ARAGHDAILAPAPDYYIDNKQSQSDEEPSGRGLVISWQRLYDFDPGISALSETERERI
                     IGLQANLWTEHVRTEDYAQAMLWPRAAVLAQLAWSGRAGDWNRFGPLLVWQIEREARL
                     GYGSSRVSLDPLARFDAGSGPGRVAVELTQPAAIGTLRYTLDGSVPQAGSPRYAGPLE
                     LAEGTRLRVRAFLDARPLGQERSWQAGIALARTRAGNDLGACAAGVELRMEDDAPTNG
                     KRLVHQAEIFNPCWIWPKAPLDGVTRLAARVGTRPYNFQLSHDAGRIQTGQPQTEAGE
                     LVVRLDGCAGPELARLPLAPLVGKQGINELGARLKAPLSGRHDLCITFARPRIDPLWV
                     LDRLTLEP"
     gene            2284..4773
                     /gene="ptsP"
                     /locus_tag="VFV30_05640"
     CDS             2284..4773
                     /gene="ptsP"
                     /locus_tag="VFV30_05640"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX4847605.1"
                     /translation="MITIASPLTGWVSPLGDVPDAAFAQKMLGDGLAVDPVKGSLRAP
                     GDGTIVSVHSAGHAVTIALDAGPVVLLHLGLDTVALGGKGFDPQCSEGQQVRLGEELI
                     RFDLGFLARHARSLITPVIVTNGEEFRIVGLAGEGAIAAGDELLRLEPLVQAPVAAPR
                     SAGGPRHERSLALPLAHGLHARPAARVAELAKGFAAAIELGGEGGRMVSARSAVAMLA
                     LSLRHGSRLTIAASGDDAEAAVAALAELIESGMGEYLTVADPVVAPVAPPPLSVPDRI
                     AGITAVAGTVVGPAFPIVRTRTAPPVDAADAQAERAALDTALDQVRAGLREALANSGP
                     QQAIAEAHLVLLDDPELAAFAQRHIAAGRSAGHAWNTAIESFARPLRASADKRFAERV
                     ADLDDLAEQVLAALAGEDGSAALPPAGAILVGEDLLPSHLMRFAGAQLGGLASGAGGA
                     SSHVAIIAAGLGLPMLCGLGEVVGEIAPGTRLAIHDGALLHDPADAEVARAQQAAAVA
                     ARLRETAQRHAHEPAITRDGTAIEVFANLGSADDARRAAQGGAEGCGLLRTEFLFLDR
                     ASPPSRDEQQACYQAIADALGPRPLIVRTLDIGADKPVPWLAQPHEENPALGLRGIRL
                     QLANPALLEDQFGALLACNVPGGLKIMLPMVSELAEIVAARAVLESLAAAAGKPVPEL
                     GIMIETPAAALLAPVLAASADFFSIGSNDLSQYTQARDRTASSVACGLDGLHPAVLRL
                     VAATVAGAEQHGRWAGLCGSLAADRLAIPVLIGLGLSELSVPPAALAVTKQVIRGLDL
                     EQCRGLARQALDAPDAQTVRRLSSALLETCP"
     gene            4770..6488
                     /gene="nagE"
                     /locus_tag="VFV30_05645"
     CDS             4770..6488
                     /gene="nagE"
                     /locus_tag="VFV30_05645"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HEX4847606.1"
                     /translation="MKFGLDRLQPLGRALMLPIAVLPVAGLLLRLGQPDLLDIGFIAA
                     AGAAIFDNLGLLFALGIGVGLARDNNGAAGLAGLVCFIVATRGAQALLEVPASVTAAV
                     PADLAAMAEAAWAQKAIAKLSVPIGILSGLVAGWAYNRFSTIRLPSYLAFFGGKRFVP
                     IVAGLAGLVIAALFGEGFAAIDAGMTRFSQLVIGSGGLGLMAYGLLNRLLIVTGLHHI
                     LNNVAWFIVGEHNGATGDLNRFFAGDPAAGAFMAGFFPVMMFALPAACLAMYHAALPE
                     RRKEVGGMLGSMALTAALTGVTEPIEFTFMFLAPALYLFHALMTGVAMALLDWLQVRL
                     GFGFSAGLFDYVLNYGKATRPLALLPIGLAYAAIYYGAFRFAIARFDLKTPGREPLGE
                     DPGQGGDAAGELGPAYLAALGGAGNLAAIDACTTRLRLRVHDQAKADEAWLKALGARG
                     VLRLSDHDLQVVIGPEADLIAGQMRAAAGTLNSLVGAGLASDSAAQAGEAIDPVPWVQ
                     ALGGRANITASGAASSRVWIDLADSDRVDRAGLEALGVRMIARDGRGLQLLVADASAL
                     AAALQA"
     gene            complement(6479..8170)
                     /locus_tag="VFV30_05650"
     CDS             complement(6479..8170)
                     /locus_tag="VFV30_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX4847607.1"
                     /translation="MREWLEGARNAARSGRWDEAQGLARRLLAADPHSLAAIEILVLA
                     SRARGDLARAEALLRQAIAIDPAQRWPYGDLARLLIDQARGAEAETLAWQALRADPGN
                     ADALMLLGRLELERGLLIEAEGHLAAAIALVGRHPQLVLLLARAIMFQSRIEEARRLF
                     GEALAGQPQSIEGLAALAELEERAGNFDAAVQLLDRAEPLARRQGDNLDLQRAVLLAR
                     MGRQRDALVLLEGAGALSGAARLQRGRLRDQLGQHGAAWDDWTAAKQQIAAQTGRGYD
                     RGAVEREAAVLRSFAERLPAGAAAATTDGAQPVFIVGFPRSGTTLVEQIAAAHDQVHA
                     GGELPFGPELRDLAASRLGGPTALHAWLEQGGDETAGQLAQVLRDHYLSRAASYRLTG
                     GKTALFTDKMPLNELWLPLIRLAFPAARIVRIVRHPLDILVSALSHDMTHGHNFAYRL
                     EDAVHYMALIDRQLRAYRQSGLRIDHVLRYEDLIGDQKGETARLMAVLGLELQDAQLS
                     FHRAERIAPTPSYAQVREPLNKRSIARWHHYAGQLAPARVALGEMIAALGYEDQA"
     gene            8387..>9031
                     /locus_tag="VFV30_05655"
     CDS             8387..>9031
                     /locus_tag="VFV30_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methylproline demethylase"
                     /protein_id="HEX4847608.1"
                     /translation="MSSDPLLQPFQLKHLTLKNRLIVTAHEPAYPEDGMPKGRYRAYH
                     VERARGGVALTMTAGSAAVSRDSPPVFNNILAWQDEVVKWTRELTDAVHEAGSAIMIQ
                     LTHLGRRTRWDKADWLPVVSPSHHRERAHRAFPKKIEDWDIARIIRDFGDAAERMQAA
                     GMDGVEVEAYGHLLDQFMSPLTNELDGPYGGSLDNRMRFTFDVLTEMRRRVGPDF"
BASE COUNT         1356 a         3027 c         3129 g         1519 t
ORIGIN      
        1 ggtacccgca atggctgcgg gattcctcag gaacggttcg ttgatccttg ccgtcgtgct
       61 ttcgggcgcg acggcagccc ggggcgaagc caccaaggct tcgcccctgc tgtttccggc
      121 gccggccaag gttgaaccgg aagccggagc cttcgcgctt gccggggcgc aagttcacgt
      181 tgacgatgcc gggggcaggg ccgctgcagc gcggcttgcc gaactgctca ggcgctctgg
      241 caaacccgct cctgcccttg cagccaaggg caagatccgt ttcgtgcgcg atccatcaat
      301 tgcatcggct gaaggttacc ggcttaccgt gcggcccgaa gcggtcacgc tggccgcgag
      361 cggcgatgcc gggctgttct atggcgcgca ggcgctgtgg cagttgatgg tcgagtcacg
      421 gggcggcacg atcggcgccg tgacgatcac cgatgcgccc aaatatgcct ggcgcggcac
      481 catgctcgat tcggtgcggc acatgcagag ccccgccttc atcaagcagg tgatcgaccg
      541 gatggcgctg gtcgggctca accgttttca ctggcatctg accgacgatc agggctggcg
      601 gttcccggtt ccgggctatc cccgcctgac ccaggtggca gcctggcgcc agcaggcggg
      661 ggccgcgggc ttcgatccgc agactggcca accggtccgc tacggcggct attatacccc
      721 ggcgcaaatt cgcgagatcg tggcctatgc cagggcgcgg cacatcgaag tggtgccgga
      781 agtcgatttt cccggccatg ccaccgcagc aattgccgcc tatcccgggc tggcatcgaa
      841 tttcgctccg cccgccgcgc cgggtgcgga ctggggggtc tatcacaaca ttatcaagcc
      901 cgacgaacag ggcctgaaat tcgcccgcgc cgtgctggat gaggtgatgc ggctgtttcc
      961 cggcaagcat atccacgttg gcggcgatga ggcggtgaag gactactgga aggccaatcc
     1021 cgcgatccag gcgcagatca gggcgctggg gctgaaggac gagaacgccc tgcagggctg
     1081 gttcatggcc cagcttggcg cgcatgtagg caggcaggga cggcgcatcg tcggctggga
     1141 cgaggtgctg gaaggcgaag tgccgggcga tgccgtggtc atgtcgtggc gcggggcgag
     1201 cggggcgctg gtcgccgcgc gggccggaca cgatgcgatc ctggcgccgg cgcccgacta
     1261 ttacatcgac aacaagcaga gccagtcgga cgaagaacct tcggggcgcg ggctggtgat
     1321 cagctggcag cggctttacg atttcgatcc cggcatttcg gcgctgagtg aaaccgaacg
     1381 cgagcggatc atcggcctgc aggccaactt gtggaccgaa catgtccgca ccgaggatta
     1441 tgcccaggcc atgctctggc cgcgcgccgc cgtgctcgcc cagctggcct ggagcggcag
     1501 ggccggggac tggaaccggt tcggtccgct gctggtctgg cagatcgagc gcgaagccag
     1561 gctgggctat ggttcaagcc gggtcagcct tgatccgctg gcccggttcg atgccggatc
     1621 ggggccgggc agggtggcgg tcgaactgac ccagcccgcg gcgatcggca cgcttcgcta
     1681 tacgctggat ggcagcgtgc cgcaggcggg ttcgccgcgc tatgccgggc cgctcgaact
     1741 ggccgaaggc acccggctca gggtccgcgc ctttctcgat gcgcggccgc ttggccagga
     1801 acgcagctgg caggcgggga tcgcgctagc ccgcacccgg gcgggcaacg atctgggcgc
     1861 ctgcgcggcc ggggtcgagc tcaggatgga agacgatgcc ccgaccaacg gcaagcggct
     1921 ggtccaccag gccgaaatct tcaatccctg ctggatctgg cccaaggcgc cgctcgatgg
     1981 cgtcacccgg cttgccgcgc gggtcgggac gcggccgtac aacttccagc tcagccatga
     2041 tgccggacgg atccagaccg gccagccgca gaccgaagcg ggcgagctgg tggtgcggct
     2101 cgatggctgc gccgggccgg aactggcccg cctccctctg gccccgctgg tcggcaagca
     2161 gggcatcaac gaactggggg cacgcctcaa ggcgccgctc tcgggcaggc acgacctgtg
     2221 catcacattc gcccggccgc ggatcgatcc gctctgggtg ctggacaggc tgacactcga
     2281 accatgatca ccatagcaag cccgctcacc ggctgggtca gcccgcttgg cgacgttccc
     2341 gatgctgcgt ttgcccagaa gatgctgggc gatggcctgg cggtcgatcc ggtcaagggc
     2401 tcgctgcgtg cgcccggcga tggtacgatc gtttcggtcc acagtgccgg ccacgcggtg
     2461 acgatcgcgc ttgatgccgg gccggtggtg ctgctgcacc ttgggctcga tacggtggcg
     2521 ctaggcggta agggctttga tccgcagtgc agcgaagggc aacaggtgcg ccttggcgag
     2581 gagctgatcc ggttcgacct tggcttcctg gcccgccatg cccgcagcct gatcaccccg
     2641 gttatcgtca ccaatggcga ggaattccga atcgtcggct tggcgggcga gggggcgatt
     2701 gcggcgggcg acgaattgct gcggcttgaa ccgctggtcc aggcgccggt cgccgcgccg
     2761 cgcagtgctg gcggtccgcg ccacgaacgc agcctggcgc tgccgctggc ccatgggctg
     2821 cacgcccgcc cggcggcgcg ggttgccgag ctggcgaagg gctttgccgc cgcgatcgaa
     2881 ctgggcggtg aaggcgggcg gatggtttcg gcgcgcagcg cggttgccat gctggcgctg
     2941 tcgttgcggc atggctcgcg cctgacgatt gccgccagtg gcgatgatgc cgaagcggcc
     3001 gtggctgccc tcgccgagtt gatcgaaagc ggcatgggcg aatatcttac cgtggccgat
     3061 ccggttgttg cgccagtcgc accgccgccg ctatcggtgc ccgatcggat tgcagggatc
     3121 accgccgtgg ccgggaccgt ggttggcccg gcctttccga tcgtccggac cagaactgcg
     3181 ccgccggtcg atgccgcgga tgcccaggcc gaacgcgccg cgctcgacac cgcgctcgat
     3241 caggttcgcg ccgggctgcg cgaggcgctg gccaacagcg ggccgcagca ggcgattgcc
     3301 gaagcccacc tggtgctgct ggacgacccc gagctggccg cattcgcgca aaggcacatc
     3361 gccgccgggc gcagcgccgg tcatgcctgg aacacggcaa tcgaaagctt tgcccggccc
     3421 ttgcgcgcct cagccgacaa gcgctttgcc gaacgcgtcg ccgatctcga tgaccttgcc
     3481 gagcaggtgc tggcggcgct ggcgggtgag gatggcagtg ccgccctacc gcccgccggg
     3541 gcgatcctgg tgggcgaaga cctgctgcca tcgcatctga tgcgctttgc cggggcgcaa
     3601 ctgggcgggc tcgcttcggg tgcgggcggg gcaagctcgc acgttgcgat catcgccgcc
     3661 gggcttggtc tgccgatgct gtgcgggctg ggcgaggtgg tgggcgagat cgccccggga
     3721 acacgcctcg cgatccacga tggcgccttg ctccacgatc ccgccgacgc cgaggttgcc
     3781 cgcgcccagc aggccgccgc cgtcgccgcc aggctgcgcg aaaccgcgca gcgccatgcc
     3841 cacgaaccgg cgatcacccg cgatggcacg gcgatcgaag tcttcgccaa tctcggttcg
     3901 gccgacgatg cccgccgcgc ggcgcagggc ggggccgagg gttgcggatt gctgcgcacc
     3961 gaattcctgt ttctcgaccg cgccagcccg cccagtcggg atgaacagca ggcctgctat
     4021 caggcgattg ccgatgccct ggggccgcgt ccgctgatcg tgcgcacgct cgatatcggc
     4081 gcggacaaac cggtaccctg gctcgcccag ccgcacgagg aaaacccggc gctgggcctg
     4141 cgtggcatcc gcctgcaact ggccaacccg gctttgctgg aagaccagtt tggcgccctg
     4201 ctggcctgca atgtacccgg cgggctcaag atcatgctgc cgatggtttc cgaactggca
     4261 gaaatcgtcg cagcgcgcgc cgtgctcgaa tcgcttgccg ccgcggccgg aaagccggtg
     4321 cccgaacttg ggatcatgat cgaaaccccg gccgcggcgc tgctcgcccc agtgctggcc
     4381 gccagtgccg atttcttctc gatcggcagc aacgacctca gccagtatac acaggcgcgc
     4441 gatcgcaccg catcgagcgt ggcgtgtggc cttgacggac ttcacccggc ggtgctgcgg
     4501 ctggttgcgg caaccgttgc gggcgccgaa cagcatggcc gctgggctgg actgtgcggc
     4561 tcgctcgcgg ctgaccggct tgccatcccg gtgctgatcg ggctggggct gagcgaactt
     4621 tcggtgccgc ccgccgcact cgccgtgacc aaacaggtga tccgagggct cgatcttgaa
     4681 cagtgtcgcg ggctggcccg ccaggcgctg gacgcgcccg atgcgcaaac cgtgcgccgg
     4741 ctgagcagcg cattgctgga gacctgccca tgaaattcgg cctcgatcgc cttcaaccgc
     4801 tgggccgcgc gctgatgctg ccgatcgcgg tgctgcctgt ggccggcctg cttctgcggc
     4861 tgggccagcc cgatctgctc gacatcggct ttatcgctgc tgctggcgcc gcgattttcg
     4921 acaatctcgg cctgctcttc gcgcttggca ttggcgtcgg cctggcccgc gacaacaacg
     4981 gcgcggcggg gcttgccggg ttggtctgct tcatcgtcgc cacccgcggc gcgcaggcct
     5041 tgctcgaggt gcccgctagc gtcactgccg ccgtgcccgc cgatcttgcg gccatggccg
     5101 aggcggcctg ggcccagaag gcaatcgcca agctttcggt gccgatcggg atcctttcgg
     5161 gcctggtggc aggctgggcc tataaccgct tcagcacgat ccgcctgccc tcgtacctcg
     5221 cctttttcgg gggcaagcgg ttcgtcccga tcgttgccgg gctggcgggc ctggtcatcg
     5281 ccgctctgtt cggcgaaggc tttgccgcga tcgatgccgg gatgacgcgc ttttcccaac
     5341 tggtgatcgg atcgggcggg ctggggctca tggcctatgg cctgctcaac cggttgctga
     5401 tcgtcaccgg attgcaccac atcctcaaca acgtcgcctg gttcatcgtt ggcgaacaca
     5461 acggggcgac cggcgatctc aaccgctttt tcgctggcga tccggcagcg ggcgccttca
     5521 tggcgggctt cttcccggtg atgatgttcg cgctgccagc cgcgtgcctg gcgatgtacc
     5581 acgccgcctt gccggaacgc cgcaaggaag tgggcggcat gctgggttcg atggcgctca
     5641 ccgcagcgct gacaggggtg accgaaccga tcgagtttac cttcatgttc ctcgctccgg
     5701 cgctctacct gttccacgcg ttgatgaccg gggtggcgat ggcgctgctc gattggctgc
     5761 aggtgcggct tggtttcgga ttttcggccg ggctgttcga ctatgtgctc aactatggca
     5821 aggccacccg gccgctggcg ctgctgccga tcgggctggc ctatgccgcg atctattatg
     5881 gcgcgttccg ctttgcgatc gcgcgcttcg atctgaagac cccggggcgc gagccgctgg
     5941 gcgaggatcc ggggcagggc ggtgatgccg ctggcgaact tggcccggcc tatctcgccg
     6001 cgctgggcgg ggcgggcaac cttgctgcga tcgatgcctg caccacccgg ctgcggctgc
     6061 gggtccacga tcaggccaag gccgacgagg catggctgaa ggcgctgggc gcgcgcggcg
     6121 tgctgcgcct gtccgatcac gatctgcagg tggtgatcgg ccccgaagct gatctgattg
     6181 ccgggcagat gcgcgctgcg gcggggacct tgaacagcct ggtcggcgcc ggtctggctt
     6241 cagacagcgc ggcacaggcc ggcgaggcga tcgatcccgt gccgtgggtt caggcgctgg
     6301 gcggacgcgc caacatcacc gcttcgggtg ccgcgagcag ccgggtctgg atcgatctgg
     6361 cggacagcga ccgggtcgat cgggccgggc tcgaagccct gggggtgcgg atgatcgcgc
     6421 gggacgggcg cgggctgcaa ctgctggtcg ccgatgcgtc ggcactggcc gcggcgcttc
     6481 aggcctgatc ttcatagccc agggctgcga tcatttcgcc cagcgccacg cgggccgggg
     6541 ccagctgtcc ggcgtaatgg tgccagcgcg cgatcgacct tttgttcagt ggttcgcgga
     6601 cctgggcata gctgggcgtg ggcgcgatcc gctcggcgcg gtgaaagctc agctgcgcgt
     6661 cctgcagttc cagtcccagc accgccatca gccgcgcggt ttcccccttc tgatcgccga
     6721 tcaggtcttc ataacgcagc acgtgatcga tccgcaggcc tgattgccga taggcgcgca
     6781 gctgccgatc gatcagcgcc atatagtgca ccgcatcttc cagccggtag gcgaagttgt
     6841 ggccgtgggt catgtcgtgg ctcagggccg aaaccagaat gtcgagcgga tggcgcacaa
     6901 tccgaacgat ccgcgccgcc ggaaaggcca ggcggatcag cggcagccac agctcgttaa
     6961 gcggcatctt gtcggtgaac agcgcggtct tgccgccggt aagccgatag gatgcggcgc
     7021 gggagaggta atgatcgcgc agcacctggg cgagttgacc ggccgtttca tcgccgccct
     7081 gttccagcca ggcgtgcagg gccgtgggtc cgcccagtcg gctggcggca agatcgcgca
     7141 gttcgggacc gaacggcaac tcgccaccgg cgtgcacctg gtcgtgcgcg gcggcgatct
     7201 gttcgaccag ggtggtaccc gatcgcggaa agccgacgat gaaaaccggt tgcgcaccat
     7261 cggttgtggc cgccgccgca ccagccggca gtcgttcggc aaatgaccgc agcaccgcgg
     7321 cttcgcgctc aaccgcgccg cgatcatagc ctcgcccggt ttgcgccgcg atctgctgct
     7381 tggccgcggt ccaatcgtcc caggccgcgc catgctggcc caactgatcg cgcaggcgcc
     7441 cgcgctgcag ccgggcggca cccgacagag cgcccgcgcc ttcaagcagc accagggcat
     7501 cgcgctgccg gcccatcctc gccagcagca ccgcgcgctg caggtcgaga ttgtcgcctt
     7561 gccggcgcgc cagcggttcg gcccgatcaa gcagttgcac ggccgcatcg aagttgccgg
     7621 cgcgttcttc aagttcggcc agcgcggcca gtccctcgat cgattgcggc tggccggcca
     7681 gcgcttcgcc gaacaggcgc cgggcctcct cgatccggct ctgaaacatg atcgcgcggg
     7741 ccagcaacaa caccagttgc ggatgccggc cgaccagggc gatagccgct gcaagatgcc
     7801 cttcggcttc gatcagcaag ccgcgttcaa gctcgagccg gcccagaagc atcagcgcat
     7861 cggcgttgcc gggatcggcc ctcagcgcct gccaggccag tgtctcggcc tcggcaccgc
     7921 gggcctgatc gatcagcagc cgggccagat cgccataagg ccagcgctgc gcgggatcga
     7981 tcgcgatggc ctggcgcaag agcgcttcgg cccgggcaag atcgccgcgc gcccggctgg
     8041 ccagcaccag gatctcgatc gcggcaagcg aatgcggatc ggcggccagc aagcgccggg
     8101 ccagcccctg tgcctcgtcc cagcggccgg aacgggccgc gtttctggcg ccttcaagcc
     8161 actcgcgcat tcacttcctg aagggcaaaa gccggcgcgt ttcaatcaaa atcgatggat
     8221 cgcggcactg ccgcctgcct gggtgcggca ttgccccgca tcgccgggaa atccggcttg
     8281 cctgccatcg ctggcaaatc acagaccatt ggagaatgta atctttactg taggggccag
     8341 ttcttgttga accggggcgg tcccttccag tcgagtcccc gccgccatgt ccagcgatcc
     8401 gcttctccag ccctttcagc tcaagcatct gacgctgaag aaccgcctga tcgttaccgc
     8461 gcacgaaccg gcctatcccg aagacgggat gcccaagggc cgctaccggg cctatcatgt
     8521 cgaacgggcg cggggcgggg tggcgctgac catgaccgcc ggttctgcag cagtctcgcg
     8581 cgatagcccg ccggtgttca acaacatcct cgcctggcag gacgaggtgg tgaaatggac
     8641 ccgcgagctt actgacgcgg tacatgaggc cggatcggcg atcatgatcc agcttaccca
     8701 cctggggcgg cgtacccgct gggacaaggc cgattggctg ccggtggtct cgccctcgca
     8761 ccaccgcgaa cgcgcgcacc gcgccttccc caagaagatc gaggattggg acatcgcccg
     8821 gatcatccgg gacttcggcg atgccgccga acggatgcag gcggcaggca tggatggggt
     8881 cgaggtcgag gcctatggcc acctgctcga ccagttcatg tcgccgctga ccaacgagct
     8941 cgacgggccc tatggcggca gcctcgacaa ccggatgcgc ttcaccttcg atgtcctcac
     9001 cgaaatgcgc cgtcgcgtcg ggcccgattt c
//
LOCUS       DASUDV010000262         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_560732,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000262 DASUDV010000000
VERSION     DASUDV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560732"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFV30_05660"
     CDS             complement(<1..645)
                     /locus_tag="VFV30_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HEX4847609.1"
                     /translation="MRMAYLIIAHHDRAGLARLIGALLPPGAPDLAVIHADARSDLWV
                     ELRENPLSNDPRVHVLPDPVPVIWGHWSQVEAARRLIAAALRLGCDFAHTLSGVDWPV
                     ADRTRIIADIEAAPAGTCFVEAMPRVQEERMQVYRLDTRWLRLDPRRDRLAYAATWEL
                     RRLSRIGQHLADRLGMARSRPLGPWHKGSCWWSLPERALTQAEQDLGQLIANGRL"
     gene            complement(652..2112)
                     /locus_tag="VFV30_05665"
     CDS             complement(652..2112)
                     /locus_tag="VFV30_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4847610.1"
                     /translation="MSIRRAALWSLASQYASFAIQFVTSIIISRLYLLPADVGLFSIA
                     LAAAMMVSIFQDMGFSRFITGQPQMRMEHIRDYAAVAVGAGVLVMAVLLGAAVPLASF
                     YGEPGLSGLMMIIAASFLVQAFSIVPVALLTRELDFRRLFTVNAGSALAGALASIGLA
                     AKGFGPSALAWGMLASALFRVIVALGFRPVLPGVIRRFETIRPLLGFSSSSFLISLSG
                     SIGQRSQDLIVGRILGTAATGLFTRASALAGQMSTLVVGAINSVFYPAFARKRDAGEP
                     LGEPYLHLIACNTALNFAAAIGLALAAEPLVHLLYGPRWMEVAPLLRWTAIAEVFFVA
                     VPLQMDIPILLGRIRTLVWINFLDTALTVALLAIFARWGLETAAISRVVVAVLWFAIY
                     ITYIKRLLGLRTRQLADVYLRSAVVGLAAGLPLALAIHFEWFGTIMGFLPLLALSLGG
                     VASWLLAMIIVRHPAREEIRMILDRLAAGLLRRKAA"
BASE COUNT          456 a          709 c          707 g          297 t
ORIGIN      
        1 caatcggccg ttcgcgatca actgacccag gtcctgttcc gcctgggtga gcgcgcgttc
       61 gggcagcgac caccagcacg agcccttgtg ccagggcccg agcggccggc tgcgcgccat
      121 gcccagtcga tcggccaggt gttgaccgat ccgtgacagg cggcgcagtt cccaggttgc
      181 ggcgtaggcc agccgatcgc gccgcggatc aagccgcagc cagcgggtat caagcctgta
      241 aacctgcatc cgttcttcct ggactcgagg catggcctcg acaaagcagg tgccggcagg
      301 tgccgcctca atatcggcga tgatccgggt ccgatcggcc accggccagt cgaccccgct
      361 cagcgtatgg gcaaagtcgc agcccagccg cagtgcagcg gcgatcaacc gccttgcggc
      421 ttctacctgg ctccagtgcc cccagatgac cggaaccgga tcgggcagga catgaacccg
      481 cggatcattg gataagggat tttcacgcag ttcgacccac aggtccgatc gggcatcggc
      541 atggatcacg gccaggtccg gtgcgccagg cgggagcagc gccccgatca gcctggccag
      601 gccggcccgg tcgtggtgcg cgatgatcag ataggccata cgcatggctg gtcaggcggc
      661 tttgcgccgc agcaggccag cagcaagccg atcgaggatc atcctgatct cttcgcgcgc
      721 gggatgccga acaatgatca tggcaagcag ccagctcgca accccgccca ggctaagcgc
      781 cagcagcggc aggaagccca tgatcgtgcc gaaccattcg aagtggatcg ccaaggcaag
      841 tggcagcccg gctgccaagc ccaccacggc gctgcgcaaa tagacatcgg ccaactggcg
      901 ggtgcgcagg ccaagcaggc gcttgatata ggtgatgtag atcgcgaacc acagcacggc
      961 caccacaacc ctgctgatcg cggcggtttc cagcccccag cgcgcgaaga tcgcgagcag
     1021 tgccaccgtc agcgcggtat cgagaaagtt gatccacacc agcgtgcgga tccgccccag
     1081 caggatcgga atatccatct gcagcggcac cgcaacgaag aatacctcgg cgattgctgt
     1141 ccagcgcagc aggggggcaa cttccatcca gcgcggacca taaagcagat ggacgagcgg
     1201 ttcggcggcg agcgcgagcc cgatcgccgc agcgaaattg agcgccgtgt tgcaggcgat
     1261 gaggtgcagg tacggttcgc ccagcggctc acctgcatcg cgcttgcggg cgaaggccgg
     1321 gtagaacacg ctgttgattg ccccgaccac cagggtcgac atctgcccgg cgagagcgga
     1381 agcgcgggtg aacagaccgg ttgccgcggt tcccaggatc cggccgacga tcaggtcttg
     1441 cgagcgctgt ccgatcgatc ccgaaagact gatcaggaac gaggaagaac tgaagcccag
     1501 cagcggccgg attgtctcga accggcggat caccccgggc aggaccgggc gaaagcccag
     1561 cgccacgatc actcggaaca gggcgctggc cagcatgccc caggcaaggg cagacgggcc
     1621 gaatcccttc gcggcaaggc cgatcgaagc cagggcaccg gccagcgcgc tgccggcgtt
     1681 gaccgtgaac agcctgcgaa aatcgagttc gcgcgtcagc agtgccacgg gcacgatcga
     1741 gaaggcctgg accaggaagc ttgcggcgat gatcatcatc agcccggaaa gccccggctc
     1801 gccataaaag cttgcgagcg gcaccgccgc gcccagcagc accgccatca ccagaacacc
     1861 ggcgccaacg gccactgcag catagtctcg gatgtgttcc atgcgcatct gcggctggcc
     1921 ggtgatgaac cggctgaagc ccatgtcctg gaagatcgat accatcatcg ccgcagccag
     1981 cgcgatcgag aacagcccga catcggcagg cagcagatag agccgcgaaa tgatgatgct
     2041 ggtgacgaac tggatcgcaa acgaggcgta ttgcgaggcg agcgaccaca gggcggcgcg
     2101 gcggatagac atggttcagc ccagcaccag ggtacttgcc agcagccagg cgcggcgcgg
     2161 ttcgccaaa
//
LOCUS       DASUDV010000263         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1554839,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000263 DASUDV010000000
VERSION     DASUDV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554839"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..600)
                     /locus_tag="VFV30_05670"
     CDS             complement(100..600)
                     /locus_tag="VFV30_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847611.1"
                     /translation="MRRALRILPFLVLAAGSAGQAAVDDLSYETAAVSQSFAAIYAPA
                     GSDLALGQAALAAGDYPRAVRLLEPLADGSRNSQIKLLAGYANLGAGRIGKAERHFGA
                     ALKLSYDAPFAHLGLGLAAVARGDRAEAALRLERIEQARVRCAQACSRAAQFDQAAQS
                     LRRALD"
     gene            complement(783..1193)
                     /locus_tag="VFV30_05675"
     CDS             complement(783..1193)
                     /locus_tag="VFV30_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847612.1"
                     /translation="MTRLPTWRIALLRLCYLVIAGGLSTIVVPTLIDLPTDWTNSRAV
                     VVSFLTAMMTLCALGVFRPIAMLPVMVFELIWKFVFMFRIALELWLGGRLNPAFEQTF
                     WDCVPILLFVPIIPWRLVWHRMMPRRRPDPAPAS"
     gene            complement(1217..2269)
                     /locus_tag="VFV30_05680"
     CDS             complement(1217..2269)
                     /locus_tag="VFV30_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX4847613.1"
                     /translation="MRKVLLLLCLMLFGLTGTAVRAANAPAPARDESPRVLLVVSSHG
                     RGEGKIQPGFEMDELTQAWLVLRANGFAVDIASPEGGRVQADEFDPAKPYNAAFLADP
                     EAKARLDATQRLDPAMAGRYAAIMVIGGKGAMFDLPFSQILQDLLLEADRRGAVIAAV
                     CHGPAVLARLRQPDGSNWVSGRLLTGFSDEEEAMFGKRWVPHFPFLLETELRRLDARF
                     DEAPIMLPRVAVDGRLVTGQNPYSVAGAADAVVRLLGQTPVARTPWPDEWGLTLVARA
                     LDGDEAPLIAGLARGDGSVDAPLVAVWGYYRALQAGENRAQLAQALRIMEIAYPHFPQ
                     PELRDAISETRKRLAP"
     gene            complement(2379..>2549)
                     /locus_tag="VFV30_05685"
     CDS             complement(2379..>2549)
                     /locus_tag="VFV30_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4847614.1"
                     /translation="ARRMTLTELAERVDITLANLSILKTGKAKAVRFSTLEAICEVLE
                     CQPGDLLGYQPD"
BASE COUNT          440 a          839 c          886 g          384 t
ORIGIN      
        1 ctgtctcgga cggatgaacg gacgcgaacg cccggtcggg gcgggccatt ggcccagacc
       61 cccggtaaag tcaagtcccg gcgcggcggc gcggcgggat cagtccagcg cgcggcgcag
      121 cgactgggcg gcctgatcga actgggcggc gcggctgcag gcctgggcgc agcggacacg
      181 ggcctgttcg atccgctcca gccgcagcgc ggcctcggcc cggtcgcccc gggcaacggc
      241 ggcgagaccc agcccgagat gggcgaacgg cgcatcgtag ctgagcttca gggctgcacc
      301 gaaatgccgt tctgccttgc cgatccggcc cgcgccgagg ttggcatagc cggccagcag
      361 cttgatctgc gaattgcgcg atccgtcggc cagcggctcc agcaggcgca ccgcgcgcgg
      421 ataatcgccc gcggccagcg cggcctggcc cagcgccagg tccgacccgg cgggcgcgta
      481 gatcgcggcg aacgactgcg agacggcggc ggtttcgtag gacaggtcgt ccaccgcggc
      541 ctgtccggcc gagccggcgg cgagaaccag gaacggaaga atgcgaagtg cacggcgcat
      601 ggtacgactc cttcggcgat cttgtgatgg cgcggaggct atcacgggat ctgtggatct
      661 ccaaattcct gccgcgcaac gaaaaacggg gcggagtcca ggataaggac tccgcccctg
      721 agcgtccggg ccgtggggac ttcgggggga ctgaccggcc cggatgaagc tgtgcaggcc
      781 gctcagctgg ccggggccgg atcaggccgg cgccgcggca tcatccggtg ccagaccagc
      841 cgccacggga tgatcggcac gaacagcagg atcggcacgc aatcccagaa ggtctgctcg
      901 aaggctgggt tcagccggcc gcccagccac agttccagcg cgatccggaa catgaagacg
      961 aacttccaga tcagttcgaa caccatcacc ggcagcatgg cgatcggacg gaacacaccc
     1021 agcgcgcaga gcgtcatcat cgcggtgagg aagctgacca ccacggcccg gctgttggtc
     1081 cagtcggtgg ggaggtcgat cagcgtcgga accacgatcg tgcttagccc accggcgatc
     1141 acgaggtagc acaggcgcag cagcgcgatg cgccaggtgg gcaggcgggt catgccgggc
     1201 ctccgcgcgg tgccggtcat ggtgccagcc gcttgcgcgt ttcggagatc gcgtcgcgca
     1261 gttcgggttg cgggaagtgc ggataggcga tctccatgat cctcagcgcc tgagccagtt
     1321 gagctctgtt ctcgccggcc tggagggccc ggtaatagcc ccagacggcc acgagcggtg
     1381 catcgacaga gccatctccg cgcgccagcc cggcaatcaa cggagcctcg tcgccgtcaa
     1441 gggcccgggc caccagcgtc agaccccatt cgtcaggcca gggcgtgcgg gcgactggag
     1501 tctgtcccag caggcgaacc acggcatcgg cagcccccgc gaccgaatag ggattctgtc
     1561 cggtcaccag gcggccatcg accgcgaccc tgggcagcat gatcggcgct tcatcaaaac
     1621 gggcatccag ccgccgcagt tcggtttcca gcaggaaggg aaaatgcggc acccaacgct
     1681 tgccgaacat ggcttcttcc tcgtcgctaa aaccggtcaa gagacggcca ctgacccagt
     1741 tggagccatc gggctgccgc agccgggcca gaaccgccgg gccgtggcag accgcagcaa
     1801 tcaccgcgcc tcgccggtct gcttccagca gcaggtcttg caggatctgg ctgaaaggca
     1861 ggtcgaacat ggcgcccttc ccgccgatga ccatgatcgc agcgtaccgg cccgccatcg
     1921 ccggatcgag ccgctgggtg gcatcaagcc gggccttggc ctccggatcg gcaaggaagg
     1981 cggcgttgta gggtttggct ggatcgaatt cgtcggcctg aacgcgtccg ccttcggggc
     2041 tggcaatgtc gacggcgaag ccgtttgccc gcaagaccag ccaggcctgg gtcagctcgt
     2101 ccatttcgaa gccgggctgg atcttgcctt cgccgcgtcc atggctgctt actacgagca
     2161 acacccgcgg cgactcatca cgtgccggcg ccggggcatt ggccgctcgg accgccgtac
     2221 cggtcaagcc gaacagcatc aggcaaagca gcaagagaac tttgcgcatt tcggggtttc
     2281 ctttcgcatt tccttcgggc gcaaggaacc agccggcacc tttgcgggtg ccggtggtgc
     2341 cgtgctcggg tttgtcgggg tcaggcttgg ggagcgcctc agtccggctg gtagccgagg
     2401 agatcgcccg gctggcattc cagcacttcg cagatcgcct cgagggtgga gaagcggacg
     2461 gccttggcct tgccggtctt gaggatcgac aggttggcca gcgtgatgtc gacgcgttcg
     2521 gccagttcgg tcagggtcat ccgccgggc
//
LOCUS       DASUDV010000264         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1400454,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000264 DASUDV010000000
VERSION     DASUDV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400454"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VFV30_05690"
     CDS             complement(<1..1722)
                     /locus_tag="VFV30_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847615.1"
                     /translation="MKRALLLSIGGLLTLSSAWAVALASPESLLPPGFERPKARPGAA
                     AAPGGTSTPVVQPLPGAAGAAGKAVAAPAGTRLPSLREIEAMSPDELDELLGLKPKYD
                     IPGAARRSLKQVGVLAEYEGGLPAASLALQSGQLVQAVLDRNKGQLVSRWGHILLRRA
                     LASRLDAPAGMNPADFTASRAALLVRMGEGAAARAVVQDVDAGNYNPALTQAAIESYA
                     LTADITGICPVIAVQGGVRKDADWRVLRAICAAFQGDGASGMAQLERLEREGAWQKID
                     MLLARKYAGAAGKSRQAVKIEWDGVSNITPWRFALTTAVGLEPPASLMEDAPVRFAYA
                     TATAPMVGLEARAKAADRAAATGILSSAAMVDLYAQIYAQDDISGAWATSSETLREAY
                     VAASEADRLAAIRSLWDGAGDAQARYSRQVLTAYAAARLPAGDSLAPYTGDLVASMLT
                     AGLDRNAMRWLSQVEEGSLGWAQLVLVNPLRPAAVTAGALDSFHDADESDQYRKSRFL
                     VAGLAGLGRVDTGVANDFAAQVGVDLTRKTRWTRLIDQAADVNNQALVVLLAGVGMQG
                     DGWDKMTS"
     gene            complement(1719..1865)
                     /locus_tag="VFV30_05695"
     CDS             complement(1719..1865)
                     /locus_tag="VFV30_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847616.1"
                     /translation="MNKQNKVLIMGGLVAMALGALLLWAWRDGGIEPVRPLSAPAMLP
                     GEAA"
     gene            1933..2502
                     /locus_tag="VFV30_05700"
     CDS             1933..2502
                     /locus_tag="VFV30_05700"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX4847617.1"
                     /translation="MEHDATPLSDAQIRDLARRIDRPIVLVGLMGVGKSSVGKKLSAL
                     LNLPFVDADEEIEKAAQMSIPEIFAQFGEGYFRDGERRVIARLIDGSAKVIATGGGAF
                     VNDQTRELILDRAIAVWLDSDIETLLERVGRKDNRPLLKQGDPREILTRLKAEREPAY
                     AQAPIHVKSTSGPHGRSVQAVLKGIDEWL"
     gene            2493..>3119
                     /locus_tag="VFV30_05705"
     CDS             2493..>3119
                     /locus_tag="VFV30_05705"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX4847618.1"
                     /translation="MAVIPVDVAGRPYEVRIGTGLLAALVEQCQGRLRKRRVPVITDV
                     NVHARWGETVEHALHAGGHEAAWRILPPGEASKSWGQLAATVDWMLAEEVERGDHVLA
                     LGGGVIGDLTGFAAAILKRGCNFIQLPTTLLAQVDSSVGGKTAINTPAGKNLVGAFHQ
                     PSLVLADLAVLDTLPRRELLAGYAEVVKYGLLGDADFFSWCDTHGAAML"
BASE COUNT          549 a         1084 c          993 g          493 t
ORIGIN      
        1 gctggtcatc ttgtcccagc catcgccctg catgccaacc ccggccagca gcacgaccag
       61 cgcctgattg ttgacgtcgg ccgcctggtc gatcagccgg gtccagcggg tcttgcgggt
      121 cagatcgacg ccaacctgcg cagcgaaatc gttcgccacg ccggtatcga cccgacccaa
      181 gccggcaagc ccagccacaa gaaagcgcga tttgcggtac tggtcggatt cgtcggcatc
      241 atggaaactg tcgagcgctc cggcggttac cgccgctggc cgcaacggat tgaccaggac
      301 tagctgggcc cagccaagcg aaccctcttc gacctgcgac agccagcgca tcgcgttgcg
      361 atcgagacct gcggttagca tcgaggccac cagatcgccc gtatacgggg caagactgtc
      421 accggccggc agccgcgcgg cggcataggc ggtgagtacc tggcgcgaat agcgcgcctg
      481 ggcatcgccc gcaccatccc atagcgaccg gatcgcggcc agccgatcag cctcgctcgc
      541 cgcgacatag gcttcgcgca gggtttccga actggtcgcc caggccccgg aaatgtcatc
      601 ctgagcatag atctgggcgt agagatcgac catcgccgcg ctcgacagaa tcccggttgc
      661 cgccgcccga tcggccgcct tggcccgggc ttcaaggccg accatcggtg cggttgccgt
      721 ggcataggca aagcgcaccg gcgcatcctc catcagcgac gccggcggtt caagcccgac
      781 cgcggtggtc agggcaaagc gccacggtgt gatattgcta acgccgtccc attcgatctt
      841 caccgcctgg cgcgacttgc ccgccgcccc ggcatatttg cgggccagca gcatgtcgat
      901 cttctgccag gccccttccc gctcaagccg ttcgagctgg gccatgcccg aagccccgtc
      961 gccctggaag gccgcgcaaa ttgcgcgcaa gacccgccag tcggcatcct ttcgcacccc
     1021 gccttgaacc gcgatcaccg ggcagatccc ggtaatgtcg gcagtcagcg catagctttc
     1081 gatcgcggcc tgggtcagcg ccgggttgta gtttccggca tcgacatcct gcaccaccgc
     1141 ccgcgcggcc gccccttcgc ccatccgcac cagcagcgca gcgcgcgagg cggtgaaatc
     1201 ggccgggttc atgccggccg gcgcatcgag ccgcgaggcc agcgcgcggc gcagcaggat
     1261 atggccccaa cgcgaaacca gctgaccctt gttgcgatca agcaccgcct ggaccaactg
     1321 gccgctttgc agcgccagcg aagccgccgg cagaccgcct tcgtattctg ccaggacccc
     1381 aacctgtttg agcgaacggc gggccgcgcc agggatgtcg tacttgggct tgaggcccag
     1441 aagttcatcg agttcgtcgg ggctcatcgc ctcgatctcg cgcagcgacg gcaggcgtgt
     1501 ccccgctggt gctgccaccg ccttgcctgc agcgcccgca gcgccgggca gcggctgaac
     1561 cactggggtg gacgtaccgc ctggcgccgc agcggcacca ggccgggcct tggggcgttc
     1621 gaaacctggc ggaagcaggg attcgggcga tgccagagcc accgcccatg ccgaactgag
     1681 tgtcagcaga ccaccgatcg agagcagcaa tgcgcgcttc atgcggcctc tccgggcagc
     1741 atggccgggg cgctcaaggg gcgcaccggc tcgatcccgc catcgcgcca ggcccagagg
     1801 agcagcgcgc ccagcgccat cgccaccagc ccacccatga tcagcacctt gttctgcttg
     1861 ttcaacgcca cccgacagcc tcttgctcca cagcttgcaa cgcggagtag cgcatctata
     1921 ggcagccaag caatggaaca tgatgcaacc ccgttgagcg atgcccagat ccgtgatctg
     1981 gcccgccgga tcgatcgccc gatcgtgctg gtcgggctca tgggcgtggg caagtcgagc
     2041 gtgggcaaga aactttcggc actgctcaat ctgcccttcg tcgatgccga cgaggaaatc
     2101 gagaaggcag cgcagatgtc gatcccggaa atcttcgccc agttcggcga aggctatttc
     2161 cgcgatggcg aacgccgcgt gatcgcccgg ctgatcgacg gttcggccaa ggttatcgcc
     2221 accgggggcg gagccttcgt caacgaccag acccgcgaac tgatcctcga tcgcgccata
     2281 gcggtctggc tggacagcga tatcgaaacc ctgctcgaac gggtcgggcg caaggacaat
     2341 cgcccgctgc tcaaacaggg tgatccgcgc gagatcctga cccggctcaa ggccgaacgc
     2401 gaaccggcct atgcccaggc gccgatccat gtgaaaagca ccagtggccc gcacggccga
     2461 tcggtccagg cggttctgaa aggcatcgac gaatggctgt aatcccggtt gacgttgctg
     2521 gccgccctta cgaagtgcgg atcggcaccg gcctgctcgc cgcgctggtc gaacagtgcc
     2581 agggccggct gcgcaagcgc cgcgtgccag tgattaccga tgtcaatgtc catgctcgct
     2641 ggggcgagac ggtcgaacat gcgctgcatg ccggcgggca cgaggcggcc tggcggatcc
     2701 tgcctcctgg tgaagccagc aagagctggg gccagctggc ggccacggtt gactggatgc
     2761 tggccgaaga ggtggaacgc ggcgatcacg tgctggcgct aggcggcggg gtaatcggcg
     2821 atctgaccgg ctttgccgcc gcgatcctca agcgcggatg caacttcatc cagctgccaa
     2881 ccacgctgct ggcccaggtc gattcgagcg tgggcggcaa gaccgcgatc aacacgcccg
     2941 ccggcaagaa cctggtgggc gcgttccacc agccttcgct ggtcctggcc gatctggccg
     3001 tgctcgacac gctgccccgg cgcgaactcc tcgccggcta tgccgaggtc gtgaaatacg
     3061 gcctgctggg cgatgccgat ttcttctcct ggtgcgacac ccacggcgcc gcgatgctg
//
LOCUS       DASUDV010000265         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1731812,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000265 DASUDV010000000
VERSION     DASUDV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731812"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1927)
                     /locus_tag="VFV30_05710"
     CDS             complement(<1..1927)
                     /locus_tag="VFV30_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX4847619.1"
                     /translation="MAGTLAVAAVIGWTALFVLANLAAMQAGGSLAEWTAWIRDWSVP
                     VLLVAVAWLIGMRNSRREALRFGETARLLGEESARLESRLTTVNRELSLAREFISAQA
                     RDIDTLGRVAGERLSQHADKLAGLIADNGTRLDSIQTVSAAALENMEALRGHLPVIAS
                     SAKDVTNNIGNAGRTAHAQVEELITGFNRLNQFGQASERQVMALRTTVDETLAEFTRQ
                     TEQLDVLATQRFAALAEKGAEFRTQLETQEVEALAAIRNRAAAMAAELEEARAALDAQ
                     EGESLTSLRARLTAVRDESAAIGRSLREGEDHALIAWKTAIGTLENDLTDAIARVGVI
                     DEKAMASARNRLTELAKEAEEVDSRMADRDRLFVEEIERRRAEFEERQAAFITQLGEQ
                     MADLDARIAERRAAQEEHGRKLVVHSETMATQLDHFARKMEEIASLGGEAEGRVAASL
                     AALGEKLAASREALSGTDTAIAGLTDGSVRLLELIQASVQHSTRDLPEAMGVSEARLS
                     TVETRLSALKDSLADAEGKGQALSGHVSEAAGGLTQALTQVEALSTILTAEGTRQAEQ
                     VDAMRASLESVRSESLGLAEQAQAELRKAIDELNASAKAAVSGIAELSAGSISKLAAS
                     LGEESGAAIEKAMRSKAA"
     gene            2157..2459
                     /locus_tag="VFV30_05715"
     CDS             2157..2459
                     /locus_tag="VFV30_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4847620.1"
                     /translation="MAYEAGALDATLAAAAGADAVLFSELRQAFMESVARQLDLLRRS
                     RCDGNWQMSALRLKGIAATFHAEQLIALAEEAAEGAPGDPAVLRRIQNFLDDFAAA"
     gene            2605..3762
                     /locus_tag="VFV30_05720"
     CDS             2605..3762
                     /locus_tag="VFV30_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847621.1"
                     /translation="MPRAFLRVGGVTVARQQLALALELKCERIICFAAALSGEVLELQ
                     HLAEAAGASFHVIGQARALSGLVTAVDEVVALSDGLFVSIPQAAALLEEGQAVLVQPI
                     EQGLAGGFERIDLNHAAAGAMRLPGRLIERLVELPADCDAASALQRIALQAGIRQRAI
                     PTPGQDGLFWTLVRSEDEAHALEPQWIRQRTQDEGALSPSRGVALFAVRSLGPAMLHA
                     GSGARALVIAAALMALLALGSGWFNLLTLGLGFAALGWLLRECAVLLARIETESPHRR
                     AGLDSKEIYGWLTDGIIVVLTALAVEAHAGQHFIDRLFPPFMLVALLRILPRLVSPRW
                     AAWFGDRALLALVLGGAVASGAGSAAVHIAAALAALAGIVLPMAQSRLTRP"
     gene            3787..>4426
                     /locus_tag="VFV30_05725"
     CDS             3787..>4426
                     /locus_tag="VFV30_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847622.1"
                     /translation="MIETAAPANPGVSVETVLREELAHGDAMLATIAPILRHLLANDE
                     HSVFSDEIVAATRGMMSHVARQLLDRIADPAVESADGAHDPAVVAALVDGFVAHPAFL
                     AHVHALSLEWQLTQRLEARLGLDPVLSPLLQALLASTDGGVAASAMALLAAQARFCQA
                     QRRMELPLAELPGDLLHAALLGMRGYASADPEALGRAIEAEQAIRSSFDESRS"
BASE COUNT          566 a         1534 c         1541 g          785 t
ORIGIN      
        1 ccgccgcttt tgatcgcatg gccttctcga tcgccgcgcc gctttcctcg cccaggctgg
       61 cggcaagctt cgagatcgag ccggcgctca gttcggcaat accgctgacg gccgccttgg
      121 ccgaggcgtt gagttcgtcg atcgccttgc gcagttccgc ttgcgcctgt tcggccaggc
      181 ccaggctttc gctgcggacc gattccagcg aggcgcgcat cgcgtcgacc tgttcggcct
      241 ggcgggttcc ctcggcggtc aggatcgtgc tgagcgcttc gacctgggtt agggcctggg
      301 tcaggccgcc ggccgcttcg ctgacatggc cggacagcgc ctggcccttg ccctcggcat
      361 cggccaggct gtccttcagc gcgctcagcc gggtttcaac cgtgctcagg cgcgcttcgc
      421 tcacgcccat ggcttcgggc aggtcgcggg tggaatgctg gacgctggcc tggataagct
      481 cgagcaggcg gacgctgcca tcggtcagcc cggcaatcgc ggtgtcggtg cccgacagcg
      541 cctcgcggct cgcggcaagc ttctcaccca gcgcggccag gctggccgcc acccggcctt
      601 cggcctcgcc gcccaggctg gcgatctctt ccatcttgcg ggcgaagtga tcgagctgcg
      661 tcgccatggt ttcgctgtgg accaccagct tgcgcccgtg ctcttcctgc gcggcgcggc
      721 gttcggcgat ccgcgcgtcg agatcggcca tctgctcgcc cagctgggtg atgaaggcgg
      781 cctggcgttc ctcgaactcg gcgcggcgcc gttcgatctc ctcgacgaac aggcggtccc
      841 ggtcggccat ccggctgtcg acctcttccg cttccttggc cagttcggtc agacggttgc
      901 gggccgaggc catcgccttt tcgtcaatca cgccgacccg ggcgatcgcg tcggtcaggt
      961 cgttctccag tgtgccgatg gcggtcttcc aggcgatcag ggcgtggtct tcgccttcgc
     1021 gcagcgaacg gccgatcgcg gcgctctcgt cgcgcaccgc agtcaggcgg gcgcgcagcg
     1081 aagtgaggct ttcgccttcc tgggcatcga gcgcggcgcg ggcctcttcc agctccgctg
     1141 ccatggccgc cgcgcggttg cggatcgcgg ccagggcctc aacctcctgc gtttccagct
     1201 gagtgcggaa ttccgcgccc ttttcggcca gcgcggcaaa ccgctgggtc gccagcacat
     1261 cgagctgctc ggtctggcgg gtgaactcgg ccagggtctc gtccacagtg gtgcgcagcg
     1321 ccatcacttg ccgctcgctg gcctggccga actggttgag ccggttgaag ccggtaatca
     1381 gttcctcaac ctgggcgtgc gcggtgcggc cggcgttgcc gatgttgttg gtgacgtcct
     1441 tggcggagct tgcgatcacc ggcaggtggc cgcgcagggc ctccatgttc tccagcgccg
     1501 cggcgctgac ggtctggatc gaatccagcc gggtgccgtt gtcggcgatc aggccggcca
     1561 gcttgtcggc atgctgcgac aggcgctcac cggcgacccg gcccagcgtg tcgatatcgc
     1621 gggcctgggc cgaaatgaat tcacgcgcca ggctgagctc gcggttgacg gtggtcaggc
     1681 ggctttccag gcgggccgat tcctcgccca gcaggcgggc ggtttcccca aagcggagcg
     1741 cttcgcggcg gctgttgcgc atgccgatca gccaggccac cgccacaagc aggaccggca
     1801 ccgaccagtc gcggatccac gcggtccatt cggccagcga accgccggcc tgcatcgccg
     1861 ccaggttggc caagacgaac agcgcggtcc agccgatcac cgcggccacc gccagcgtgc
     1921 ccgccacgat cgccagccac gggcgggaag ggcgcgccgg ctcttcctcc cactaggtct
     1981 cctccgcgtc cgcctcggca gcggcagggg tttccggggt cggttccacc gccacgccat
     2041 cgggggcggg ggtttcgacg gcgacaatgc gggttccgcg gctcatggcg ggctgtttac
     2101 catgttgcgg cgcgggataa accccagttt aaccaccgcc tgctaggcca gcgggcatgg
     2161 cttatgaagc aggtgccctc gatgctacgc ttgccgctgc ggcgggggcg gatgccgtgc
     2221 tcttttccga gctgcgccag gcgtttatgg aaagcgtcgc gcggcagctc gatctgctgc
     2281 gccggtcgcg ctgcgatggc aactggcaga tgagcgcgct gcggctcaag ggcattgccg
     2341 ccacctttca cgccgaacag ctgatcgcgc tggccgagga agcggcggaa ggggccccgg
     2401 gcgatcccgc tgtcctgcgc cggatccaga attttctcga cgattttgca gcggcttgac
     2461 gccgcccggg cggcgcaggc cgcctagacc cttggcatga ccggcccgct cacctagaac
     2521 gcaggcccac ttgcgcaagc gcggagaaag acgctgcggg tcgccgtcct gacaatgctg
     2581 gagcctgcca cagccggcgg ccgggtgccg cgggcgttcc tgcgcgtagg cggggtcacc
     2641 gtcgcccgcc agcagctggc gctggcgctt gaactcaagt gcgaacggat catctgcttc
     2701 gccgccgcgc tttcgggcga ggtgctggaa ttgcagcatc tggccgaagc tgccggggcc
     2761 agtttccatg tcattggcca ggcccgggcc ctgtccggcc tggtgaccgc ggttgacgag
     2821 gttgttgcgc tgagcgacgg cctgttcgtc tccatccccc aggccgccgc cttgctggaa
     2881 gagggccagg cggtgctggt ccagccgatc gagcaagggc tggccggcgg gtttgagcgg
     2941 atcgacctca accacgccgc cgccggggcg atgcgcctgc cgggccgcct gatcgagcgg
     3001 cttgtcgagc tcccggccga ttgcgatgcg gcgtcggcgc tgcagcggat cgccttgcaa
     3061 gccgggatcc gccagcgcgc aatccccacg ccgggccagg atggcctgtt ctggaccctg
     3121 gtccgtagcg aggacgaagc ccacgcgctc gagccgcagt ggatccgcca gcgcacgcag
     3181 gacgaaggcg cgctcagccc gtcgcggggc gtggccctgt tcgcggtgcg cagcctgggt
     3241 ccggccatgc tccacgccgg cagcggggcc cgcgcgctgg tgatcgcggc ggcgctgatg
     3301 gcgctgctgg ccctcggttc gggctggttc aaccttctca ccctgggcct gggctttgcc
     3361 gcgctgggct ggttgctgcg cgaatgcgcg gtcttgctgg cgcggatcga aaccgaaagc
     3421 ccgcaccgcc gggccgggct cgacagcaag gaaatctatg gctggctgac cgatgggatc
     3481 atcgtcgtgc tgacggcgct ggcggtggaa gcccatgccg ggcaacactt tatcgatcgc
     3541 ctgttcccgc cgttcatgct ggtggcgctg ctgcggatcc tgccgcggct tgtctcgccg
     3601 cgctgggcgg cctggttcgg ggaccgcgcc ttgctggcgc tggtgctggg cggggcggtg
     3661 gcatcggggg ccggttcggc ggcggtgcat atcgccgcag cactggccgc gctggccgga
     3721 attgtcctgc cgatggccca atcgcggcta acgcgacctt aaccaagcag gcctatgaac
     3781 acgggcatga ttgagacagc tgcccctgcg aaccccggcg tttcggtaga gacggtgctg
     3841 cgcgaggaat tggcgcatgg cgatgcgatg ctcgctacga ttgccccgat cctgcgccac
     3901 ctgctggcca acgacgaaca ttcggtgttc agcgacgaga tcgtcgccgc cacccgcggc
     3961 atgatgagcc acgtcgcccg ccagctgctg gaccggatcg ccgatccggc cgtggaaagc
     4021 gccgatggtg cgcacgatcc ggctgtcgta gctgcgctgg ttgacggttt cgtcgctcat
     4081 ccggccttcc tggcccatgt tcacgcactg tctttggaat ggcagctgac ccagcggctt
     4141 gaagcccggc ttggccttga tcccgtgctt tcgccgctgc tgcaggcgct gctcgcctcg
     4201 accgatggcg gcgtggcggc ctcggccatg gcgctgctgg ccgcccaggc ccggttctgc
     4261 caggcccagc gccgcatgga actgccgctc gccgaactgc cgggcgatct gctccacgcc
     4321 gccctcctgg gcatgcgcgg ctatgcttct gccgatcccg aggccctggg ccgcgcgatc
     4381 gaggcggagc aggcgattcg cagctctttc gacgaaagcc gcagcc
//
LOCUS       DASUDV010000266         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2195654,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000266 DASUDV010000000
VERSION     DASUDV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195654"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFV30_05730"
     CDS             complement(<1..856)
                     /locus_tag="VFV30_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX4847623.1"
                     /translation="MRINSALTTAASSLAIGVAALSAPAFAQSTGSIEFEKEIIVTGT
                     RTGTQQVGGVSAPDTSKAKAVLTQENIERQNPGQTILDTINQIPGVSFQNNDAYGSSG
                     GTLNIRGFSADRISLTFDGIPLNDSGNYAIYSNQQVDPELIEQVNVNLGTTDVDSPTA
                     SAVGGTVNLRTKRPDRDFGVMLSGSAGQFDFMRMFAKIETGDLTASGLRAWVSASRAT
                     NDNPFNNYGKVNKQQYNAKIYLPLAGDDFISVAGHYNQNRNNFFGSLPLRLDANRTVG
                     SASINRYPA"
     gene            1186..2154
                     /locus_tag="VFV30_05735"
     CDS             1186..2154
                     /locus_tag="VFV30_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4847624.1"
                     /translation="MSGAHHHGPGVHHHRGHGHHHHDHHDHQHPVAGSGRAFALAVAL
                     NVGFVIIEVAAGLWSGSMALLADAGHNLSDVLALLLAWGASVLAARPAQAGYTYGFKS
                     SSILAAIANAALLWVALGAILFETIHRFFYPQPIEGGVMMGVAAAGIAINALSAALFA
                     SGQKADLNLRAAFVHLLADAAVSAGVVVAGAAIWLTGKAWIDPLTSLLITFAIGWGSW
                     GLLKNAVRMGLLAVPPGIDPDEVRRWLMARPGVSTVHDLHIWPMSTTETALTAHLEMP
                     GGHPGDAALHQLAEELAQRFGIDHSTLQIELGVGRCRLAGDLLRPH"
BASE COUNT          361 a          661 c          765 g          419 t
ORIGIN      
        1 tggccggata gcgattgatg ctggccgaac cgacggtgcg attggcgtcg aggcgcagcg
       61 gcagcgagcc aaagaagttg ttgcggttct ggttgtagtg gcccgcgacc gagatgaagt
      121 catcgcccgc cagcggcaga taaatcttgg cgttgtactg ctgcttattg accttgccgt
      181 agttgttgaa cgggttgtcg ttggtggcgc ggctggccga aacccaggcg cgcaggccgc
      241 tggcggtcag atcgccggtt tcgatcttgg cgaacatgcg catgaaatcg aactggccag
      301 ccgagccaga gagcatcacg ccaaaatcgc ggtccgggcg cttggtgcgc aggttgaccg
      361 tgccgccgac ggccgaggcg gtcgggctgt cgacatcggt ggtgccgagg ttgacgttga
      421 cctgctcgat cagttccgga tcgacctgct ggttcgaata gatcgcgtag ttgcccgaat
      481 cgttcagcgg aatgccgtcg aaggtcaggc tgatgcggtc ggccgagaag ccgcggatgt
      541 tgagcgtgcc gcccgacgaa ccgtaggcgt cgttgttctg gaagctgacg cccgggatct
      601 ggttgatcgt gtcgaggatg gtctggcccg ggttctgacg ctcgatgttt tcctgggtca
      661 ggacggcctt ggccttcgag gtatccggag ccgaaacgcc gccaacctgc tgggtgccgg
      721 tgcgggtccc ggtgacgatg atttcctttt cgaactcgat cgagccggtc gactgggcga
      781 aggccggggc gctcagcgcg gcgacgccga tggcgaggct gctggcggcg gtggtcaggg
      841 cagagttgat gcgcatgagg aagggattcc ctgttgtggc aagcttgcga aaacacgtgg
      901 cttctggcgc cgggcgaatc gccccaacgc cggctgcgga cgctaatagc ggcgctgcgc
      961 cgcgcactaa acagccctgt gtgacaagcc tgtgactagc ttcggccccg ggctggacca
     1021 gcgcgggaca ctcgacagaa agtttcgtaa atgccgcagt cagcataact ttgctgctta
     1081 cagtgctgca aatagcccaa ttcccccacc ttagtccggc aggcggcgat aacgattctt
     1141 gctgcactgc ggcaattgtg caacggttgg gtggaaggga ccagaatgag cggcgcgcat
     1201 catcatggac cgggggttca tcaccaccgc ggccatggcc atcaccatca cgatcatcat
     1261 gatcaccagc accctgttgc cgggtcgggc agggcctttg ccctggccgt ggcgctcaac
     1321 gtcgggttcg tcatcatcga ggtcgcggcc gggctgtgga gcggctcgat ggcgctgctg
     1381 gccgatgccg ggcataacct gtcggacgtg ctcgcgctgc tgctggcctg gggagccagc
     1441 gtgctggccg cgcggccggc gcaggccggc tatacctatg gcttcaagag ctcgtcgatc
     1501 ctggcggcga ttgccaatgc ggcgctgctg tgggtcgcgc tgggcgcaat cctgttcgag
     1561 acgatccacc gcttcttcta tccccagccg atcgagggcg gggtgatgat gggcgtggcg
     1621 gccgccggga tcgccatcaa cgccctctcc gccgcgctgt ttgccagcgg gcagaaggcg
     1681 gacctcaact tgcgcgccgc cttcgtccac ctgctggccg atgcggcggt ttcagcggga
     1741 gtcgtggtgg cgggggccgc gatctggctg accggcaagg cctggatcga tccgctgacc
     1801 agcctgctga tcaccttcgc gatcggctgg ggcagctggg gtctgctcaa gaatgcggtg
     1861 cggatggggc tgctggccgt tccaccggga atcgatccgg acgaggtgcg gcgctggctg
     1921 atggcccggc ccggcgtttc gaccgtccac gaccttcaca tctggccgat gagcacgacc
     1981 gaaaccgcgc tgactgccca tctcgaaatg cccggcggac atccgggcga cgcagcgctg
     2041 caccagctgg ccgaagagct ggcccagcgc ttcgggatcg atcactcaac cctgcagatc
     2101 gaactcggcg ttggtcgatg ccgccttgca ggcgatctgt tgcgtcccca ttaatcttgg
     2161 tgggccgata gggccggcag gggctcaacc cacgaaagga taccca
//
LOCUS       DASUDV010000267         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1510711,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000267 DASUDV010000000
VERSION     DASUDV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510711"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFV30_05740"
     CDS             complement(<1..739)
                     /locus_tag="VFV30_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX4847625.1"
                     /translation="MSTDIIARALSARQAARLASGAQGDGAGLVTFRGTSAGSSARTV
                     ESRLRDTASLKDFGAVGDGVANDTAAVQAWATHCATNHVRGYAPPGTYRLTATIAVPV
                     SEGLTLRGAGPTQTVFSMATDNTAIFDLGGAVGDTYLRYVDFGGFTLTYANVQTSAMT
                     SALPIRFSAMMFQSRVTEIRFLRGYYCMSVGSGVLAPWGCEFNNLFLDREVTGGFYDM
                     LASGASSVPNNFYGRLGADALGCTQTLF"
     gene            complement(739..1161)
                     /locus_tag="VFV30_05745"
     CDS             complement(739..1161)
                     /locus_tag="VFV30_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847626.1"
                     /translation="MPGTPIQSPASYVPSRATAFADADGTSLLVSSSNPLPVTLNGGS
                     TTPLAGSTSATGSAGPFTPVIGRPVMLMLSGTWTGLVKVMRSTDGGTTRLPLTVAGGS
                     WAQFSANCCEPVWEESDPAATLYLDVTLASGTLAYRIS"
     gene            complement(1258..>2246)
                     /locus_tag="VFV30_05750"
     CDS             complement(1258..>2246)
                     /locus_tag="VFV30_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4847627.1"
                     /translation="TALVLPRWGLNALGQVQLAQAAFLLVAGLVVLASLLRCRPGAYF
                     GYDGARFRELLKFGGGMQLGAIPTLLFDPLIRVLLTTYSGLTLTGYFEMANRIVTRFY
                     VIAIAPLGALTPHVASASLDGSGEREEVRKIYGESFSLLLFLQLPYYACLAAAMALVL
                     TLWKGQFDHLFLAVALLQLLGAFAKSLAVPAYMIYLGTGRLRWNILSQFVTCLLGLVL
                     GVGLGELFGGNGVLVAACLALGGGLQICLAALHREYRIPLRELLSARAAPGALLLAAA
                     LAASTLLALSGSTPGWWVMLGLPGLVGLAALAIVWRDPRRAALLASLSSLRK"
BASE COUNT          451 a          750 c          733 g          312 t
ORIGIN      
        1 ggaacagggt ctgggtgcac cccagcgcat cggcgcccag ccggccatag aaattgttcg
       61 gcaccgaact cgcgcccgat gcgagcatgt catagaagcc gccggtaacc tcgcgatcga
      121 ggaacaggtt gttgaattcg cagccccatg gcgccaacac gccggagccg accgacatgc
      181 agtagtagcc gcgcaggaag cggatctcgg ttacccggct ctggaacatc atcgccgaaa
      241 acctgatggg cagggcactg gtcatcgccg atgtctggac gttggcatag gtcagggtga
      301 acccgccaaa atcaacatag cgcagatagg tgtcgccaac cgcgccccca agatcaaaga
      361 tcgcggtatt gtcggtggcc atcgagaata cggtctgggt cggtcccgcg ccgcgcaggg
      421 tcaaaccctc gctgaccggt acggcgatcg tcgctgtcag ccggtaggtg cccggcgggg
      481 catagccgcg gacatggttg gtggcgcaat gcgtcgccca ggcctggacc gcggcggtat
      541 cgttggccac gccatcgccc accgcaccga aatccttgag gctggcggta tcgcgcagcc
      601 ggctctcgac ggtacgggcc gagcttccgg cgctggtgcc gcggaaagtg acgagccccg
      661 ccccgtcacc ctgcgcaccc gaggcgagcc gcgcggcctg gcgcgcgctg agcgcgcggg
      721 caatgatatc ggtgctcatc agctgatcct gtaagcaaga gtgccggaag cgagggtgac
      781 atcgaggtag agcgtcgcgg caggatcgct ttcctcccac accggttcgc agcaattggc
      841 gctgaactgc gcccagctgc cccccgccac ggtgagcggc agccgggtgg tgccgccatc
      901 ggtggagcgc atgaccttga ccagcccggt ccaggttccc gacagcatca gcatcaccgg
      961 gcggccgatc accggcgtaa agggcccggc cgagcctgta gcgctggtcg atcccgccag
     1021 cggcgtggtg ctgccgccgt tcagcgtgac cggcagcggg ttgctggagc tgaccagcag
     1081 cgaagtgcca tcggcatcgg caaaggcggt ggcccggctc ggtacgtaac tggcggggga
     1141 ttgaatcggc gttcctggca tgacggctcc tggcgaatcg gggaaccgta aactaaccaa
     1201 tatggttcat tgtaggaaag cgctatgtca ctattggctc gttcagccgt aaccgcctta
     1261 tttgcgcagc gaggacaggc tggcgagcag ggcagcccgg cgcggatcgc gccagacgat
     1321 cgccagtgct gcgaggccca ccagcccggg caagcccagc atcacccacc agcccggcgt
     1381 ggaaccggac agcgccagca aagtgctcgc cgccagcgcc gcagcgagca gcaaggcccc
     1441 gggcgcggcc ctggccgaaa gcagttcgcg cagcggaatg cggtattcgc ggtgcagcgc
     1501 cgccaggcag atctgcaggc cgccgcccag cgccaggcag gccgccacca gcaccccgtt
     1561 accaccgaac agttcgccca gccccacccc cagcaccagg ccaagcaggc aggtgacgaa
     1621 ttgcgacagg atgttccagc gcagccgtcc ggtgcccagg tagatcatgt aggccggcac
     1681 cgcgagcgac ttggcgaaag ctcccagcag ttgcagcagc gcgaccgcca ggaacaggtg
     1741 atcgaactgc cccttccaca aggtcagcac cagcgccatc gccgcggcaa ggcaggcgta
     1801 atagggcagc tgcaggaaca gaagcagcga aaagctttcg ccatagatct tgcgcacctc
     1861 ctcgcgctcg cccgacccat caaggcttgc gctggcaaca tgcggggtca gcgcgcccag
     1921 cggggcaatg gcgatcacgt agaagcgggt aacgatccgg ttggccattt cgaaatagcc
     1981 ggtcagggtc agccccgaat aggtagtgag caacacccgg atcagcggat cgaacagcaa
     2041 ggtgggaatc gcccccagct gcatcccgcc gccgaatttc agcaattcgc gaaaccgcgc
     2101 gccgtcatag ccgaagtagg cgccaggccg gcagcgcagc agactggcca gaaccaccag
     2161 tcccgcaacc agcaggaacg ccgcctgcgc tagttggacc tggcccagag cattaagccc
     2221 ccaccgcggc aggaccagcg cggtga
//
LOCUS       DASUDV010000268         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_803589,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000268 DASUDV010000000
VERSION     DASUDV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803589"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFV30_05755"
     CDS             complement(<1..285)
                     /locus_tag="VFV30_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4847628.1"
                     /translation="MSAETPPTGPLAVIVLAAGKGTRMKSDLHKVLHPIAGRPMLMHL
                     LGSLGELRPARQVIVAGDRREQIEAALAGSGADVVVQDPQLGTAHAALMAK"
     gene            348..1007
                     /locus_tag="VFV30_05760"
     CDS             348..1007
                     /locus_tag="VFV30_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4847629.1"
                     /translation="MTNHRFSIVGFDLDGTLLDTSGDLGVALNHALATIGRPAVPQAE
                     VMNLIGGGSGLMLRRALALTGGDEGVEFEALRQVLIAHYADNIAHHTALYPGAAEMLD
                     GLAASGTKIAVVTNKPEPLAVKLFDALGLSPRFASVIGGGRYPLKPAPDALHAMVEQC
                     GGGAAAFVGDTTFDTGAARAAGLPSVAVSFGFCDAPVADLGADAVIDHFDALIPALAG
                     L"
     gene            complement(1004..>2407)
                     /locus_tag="VFV30_05765"
     CDS             complement(1004..>2407)
                     /locus_tag="VFV30_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4847630.1"
                     /translation="ADAEAAKAEAARLAAASKPAEPPVAVAWKGAPELSHKDGWSFKP
                     RGRLHLDGGSVSSPGAFTSPTLGGNLRVRRARLGAEGTMPGGFGYKAEVDFANGNVAM
                     ADFFLSYSPRKTPLTIRIGNFETLNGLEQVTSSNNVSVIERASFNDAFLNSRRLGVAV
                     AVKSKSDDLRAEVAVFAAHSMDSSLDNDGWIGAARLVYAPKALGGQLHLGVNYQYRDF
                     GSNISGGTSTGSNMPSVNQLARYRARPNTQLTDVRFVDTGSFAAQGDSILGLEGAAIF
                     KGIYFAGEAQWVNTRAYSAGDIATGTDTFSGGNLAVVPTENPGFFGAYGEVGYFLTGE
                     TRGYKRGDGTWARTKVLNPVSKGGSGAFQIALRYEYLDLDDDALIAGPTNNFTTGTTS
                     LASLTTRQGRGGLQSTYLFGLNWYPSDYVRLMFNYGRVEVQGGPLAATVDPTSSLTPN
                     QREYGVNVVQTRLQIEF"
BASE COUNT          357 a          797 c          843 g          410 t
ORIGIN      
        1 cttggccatc agggccgcgt gggccgtgcc gagctgcggg tcctgcacca cgacatcggc
       61 gcccgatccg gccagcgccg cttcgatctg ttcgcgccgg tcgcccgcga cgatcacctg
      121 ccgcgccggg cgcagttcgc ccagactgcc cagcaggtgc atcagcatcg gccggccggc
      181 gatcggatgg aggaccttgt gcaggtccga tttcatgcgg gtgcccttgc ccgcggcaag
      241 aacgatgacg gccagcgggc cagtgggggg agtctcggcg ctcatggtta actgccatgc
      301 catcaaattg ttgccgtttc cacaacgctg cgccacggaa cgcggccatg accaatcacc
      361 gcttttcgat cgtcgggttc gacctcgacg gaaccctgct cgatacctcg ggcgatctgg
      421 gcgtcgcgct caaccacgcg ctggccacga tcgggcgccc ggccgtgccc caggccgagg
      481 tgatgaacct gatcggcggc ggatcgggcc tgatgctgcg ccgcgcgctg gcgctgaccg
      541 gcggggacga aggcgtggag ttcgaggcgc tgcgccaggt cctgatcgcg cattatgccg
      601 ataacatcgc ccaccacacc gcgctctacc ccggcgcggc ggagatgctg gacggtctgg
      661 ccgccagcgg cacgaagatc gcggtggtga ccaataagcc cgagccgctg gcggtgaagc
      721 tgttcgatgc gctgggtctc tcgccccgct ttgccagcgt gatcggtggc gggcgctatc
      781 cgctcaagcc cgcccccgat gcgctgcacg cgatggtgga gcagtgcggc ggcggcgcgg
      841 cggctttcgt tggcgacacg accttcgata ccggcgcggc gcgcgcggcg ggcctgccct
      901 cggtcgcggt cagcttcggg ttctgcgacg cgccggtggc cgatctgggc gccgatgcgg
      961 tcatcgacca cttcgacgcc ctgatcccgg cgctggccgg cctttagaat tcgatctgca
     1021 ggcgggtctg caccacgttg accccgtatt cgcgctggtt gggcgtcagg gacgaggtgg
     1081 gatcgaccgt cgcggccagc gggccaccct gcacttcgac ccggccatag ttgaacatca
     1141 gccggacata gtcgctggga taccagttca gcccgaacag ataggtcgat tgcaggccgc
     1201 cgcggccctg gcgggtggtc agcgatgcca ggctggtcgt gccggtggtg aagttgttgg
     1261 tcggcccggc gatcagcgca tcgtcatcga gatcgaggta ctcatagcgc agtgcgatct
     1321 ggaacgcacc cgatccgccc ttgctgaccg ggttcagcac cttggtccgc gcccaggtgc
     1381 cgtcaccgcg cttgtagccg cgggtttcgc cggtgaggaa atagccgact tcgccatagg
     1441 ccccgaagaa gccggggttc tcggtcggca ccacggccag gttgccgccg ctgaaggtat
     1501 cggtcccggt cgcgatatcg ccggcggaat aggcccgggt gttcacccac tgggcttcgc
     1561 cggcaaaata gatccccttg aagatcgccg cgccttccag gcccaggatg ctgtcgccct
     1621 gggccgcgaa gctgccggtg tcgacaaagc ggacgtcggt cagctgggtg ttcggccggg
     1681 cgcggtagcg ggccagctgg ttgaccgacg gcatgttcga tccggtggag gtcccgcccg
     1741 aaatgttgga gccgaaatcg cggtactggt aattcacgcc caggtgcagc tggccgccca
     1801 gcgccttggg cgcatagacc agccgcgccg cgccgatcca gccatcgttg tcgaggctgc
     1861 tgtccatcga atgggcggca aagacggcga cttcggcgcg caggtcgtcg ctcttgctct
     1921 ttaccgcaac ggcgacgcca aggcggcgcg aattgaggaa ggcatcgttg aagctggccc
     1981 gttcgatcac cgaaacattg ttggagctgg tcacctgttc cagcccgttg agcgtttcaa
     2041 agttgccgat gcggatggtc agcggggtct tgcgcgggct gtaggacagg aagaagtcgg
     2101 ccatcgccac gttgccgttg gcaaagtcga cttcggcctt gtagccgaac ccgcccggca
     2161 tggtgccctc ggcgcccagg cgggcgcggc gcacgcgcag gttgccgccc agcgtgggcg
     2221 aggtgaaggc gcccgggctg gagaccgaac cgccgtcgag gtgcaggcgc ccgcgcggct
     2281 tgaacgacca gccgtccttg tgggacagtt ccggcgcgcc cttccaggcc acggccaccg
     2341 gcggttcggc cggcttgctg gcagcggcaa gccgcgcggc ttcggccttg gcggcttcgg
     2401 catcggc
//
LOCUS       DASUDV010000269         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_781809,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000269 DASUDV010000000
VERSION     DASUDV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781809"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFV30_05770"
     CDS             complement(<1..411)
                     /locus_tag="VFV30_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847631.1"
                     /translation="MRKPRIAHGLFVGLADPAAIAAGQRCRDRAGRAIKVAAHVPRKA
                     ALQPGKQVALITGSQNLDRPENASGGRNPAEPGAAGKIVPARQHWRCGRNQPRPQAHQ
                     RTFGDRGAIIIQVKIEPHLDCPFCAGLASSQQQTD"
     gene            388..1779
                     /locus_tag="VFV30_05775"
     CDS             388..1779
                     /locus_tag="VFV30_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX4847632.1"
                     /translation="MRNAGLTHLLSISGLHVSAVIAAGYFLALRILGLFPWLALRVRL
                     PILAAASGAGLGIAYTLLTGAEVPTIRSCIGAVLVMLALVLGREPLSMRMVAVAAFVV
                     LLLWPEALMGPSFQMSFAAVIAIVALHGAAPLRAFTAPGEQPWTIKLARNLAMLLLTG
                     LVIELALMPIGLFHFHRAGVYGALANVIAIPLTTFVSMPLIALALLFDLFGLGGPFWW
                     LAGQSLEALLVLARFTSGMPGSTSTMPAFGAAIFGLFLSGGLWLALWHGKVRLLGLVP
                     AAIGALLLASHDAPDLLVSGDGRHVGITGLGPELVVLRESRSEFTRENLTEIAGMSGA
                     TVPLDSYPGARCNRDVCLVRLRRSRREWNLLITRSKDLIAERQLAAACDQADIVIADR
                     RLPRSCQPRWIKADRPFLDRSGGLAFDLAAGRITSVAQGQGEHGWWRPQTRAAFSAGA
                     KARVAGRDLAKPQ"
     gene            complement(1773..2465)
                     /gene="lexA"
                     /locus_tag="VFV30_05780"
     CDS             complement(1773..2465)
                     /gene="lexA"
                     /locus_tag="VFV30_05780"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX4847633.1"
                     /translation="MLTRKQHELIRFIQTRLEETGVSPSFEEMKEALDLKSKSGVHRL
                     ISALEERGFIRRLPNRARALEVLRQPDNVGAKTPAPAANSNSAAAAVVRPPEARPTPA
                     NDVIEIPLHGRIAAGVPIEALETSATLPVPAALLGAGEHYALEVSGDSMVEAGILDGD
                     YALIRRTDSARDGEIVVALVRGEEATLKYLRRENGRIRLDPANAAYEPQVYDPREVLI
                     QGKLAGLLRRYH"
BASE COUNT          398 a          778 c          870 g          507 t
ORIGIN      
        1 atccgtctgt tgctggctac tcgcgagccc ggcgcagaaa gggcaatcca ggtgcggctc
       61 aatcttgacc tggatgatga tcgcgccgcg atcgccgaag gtgcgctggt gcgcttgcgg
      121 gcgcggctgg ttccgcccgc accgccaatg ctgccgggcg ggtacgattt tgcccgcagc
      181 gcctggttca gcgggattgc ggccaccgga ggcgttctcg ggccggtcga ggttctggct
      241 cccggtgatc agggcgactt gcttgccagg ttgaagcgca gcctttcgcg gcacgtgcgc
      301 ggcaaccttg atggctcgcc cggcacgatc gcggcagcgc tggccagcgg cgatcgcggc
      361 gggatcagca aggccgacga agaggccatg cgcaatgcgg ggcttacgca cctgctgtcg
      421 atcagcgggc tgcacgtcag cgcggtgatt gccgccgggt atttcctggc tttgcggatt
      481 ttgggactgt tcccctggct ggcattgcgg gtgcgactgc cgatcctcgc ggccgcgagc
      541 ggggcggggc ttggcattgc ctataccctg ttgactggcg cggaagtgcc gacaatccgt
      601 tcctgcatcg gcgcggttct ggtgatgctt gcgctggtac tggggcgcga accgctctcg
      661 atgcggatgg tggcggtagc agcctttgtg gtgctgttgc tttggccaga agccttgatg
      721 gggcccagct ttcagatgag ttttgcggct gtaatcgcga tcgtcgccct gcatggcgcc
      781 gcgccgctgc gcgcattcac cgcgccgggc gagcagccgt ggaccatcaa acttgcccgt
      841 aacctggcca tgctgctgct gaccgggctc gtcatcgaac tcgccttgat gccgatcggt
      901 ctgttccact ttcatcgagc cggggtttat ggcgcgcttg ccaatgtgat cgccattccc
      961 cttacaacct tcgtctcgat gccgctaatt gccctggcgc tgttgttcga cctgttcggc
     1021 cttggcggtc cgttctggtg gcttgctggc cagagcctgg aggcgctgtt ggttttggcc
     1081 cggttcacgt cgggcatgcc ggggtccact tcaaccatgc ccgcgttcgg ggcagccatc
     1141 ttcggcttgt tcctgagtgg cgggctgtgg ctggcgctgt ggcatggcaa ggttcgcctg
     1201 ttgggcctgg ttccggcggc aattggggcc ttgctgctgg catcccacga tgcacccgat
     1261 ctgctggtat cgggggacgg gcgtcatgtc gggattaccg gactgggccc cgaattggtg
     1321 gtactgcgcg agagccggag cgaattcacc cgcgaaaacc tcaccgagat cgcagggatg
     1381 tccggagcta cggtgccgct ggacagctat ccaggcgcca ggtgcaacag ggatgtctgc
     1441 ctggttcggc tacggcgaag caggcgcgaa tggaacctgc tgatcacccg tagcaaggac
     1501 ctgattgccg aacggcaact agcggcggcc tgcgatcagg ccgatatagt gatcgccgat
     1561 cgccgtcttc cccgatcgtg ccaaccgcgc tggatcaagg ccgaccgacc gttccttgat
     1621 cgtagcggcg ggctcgcctt cgatctggcc gcggggcgga tcaccagcgt ggcccagggt
     1681 cagggagaac acggctggtg gcgcccgcag acaagagcgg ccttctcagc tggagctaaa
     1741 gcgcgagtcg ccggccgcga cctcgccaag cctcagtgat agcggcgcag cagcccggcc
     1801 agcttgccct ggatcaacac ttcgcgcgga tcgtacacct gaggttcgta ggcggcattg
     1861 gccgggtcga gtcggattcg tccgttctcg cgccgcaggt acttcaaggt tgcttcctca
     1921 ccgcggacaa gggcgaccac aatctcacca tcacgggcgc tgtcggtgcg gcggattagg
     1981 gcatagtcgc catcgagaat gccggcttca accatcgagt cgcccgaaac ctcaagcgcg
     2041 taatgctcgc cagcgccgag cagcgcggca ggaaccggca gggtggccga ggtttcgagc
     2101 gcttcgatcg ggacgcccgc ggcaatccgg ccgtgcagcg gaatttcgat cacatcgttg
     2161 gccggggtag gccgcgcttc gggcggacgg accaccgcgg cggcggcgct gttgctgttg
     2221 gccgcggggg cgggggtctt cgccccgaca ttgtcaggtt gacgcagcac ttcaagggcg
     2281 cgggcgcgat tgggcaggcg ccggatgaaa ccgcgttctt ccaatgccga aatcagacga
     2341 tgaacccctg acttggactt gagatcgagt gcttccttca tctcctcgaa cgagggggaa
     2401 acgccggttt cttcaagccg ggtctggata aagcggatca attcgtgctg cttgcgggtc
     2461 agcatgcgcc catcactccg ttgggacgga acagggccat tgcaagaatg ttcccgcgcc
     2521 gttcagtgaa cgaatgagga acaagtatgc aac
//
LOCUS       DASUDV010000270         5555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1930518,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000270 DASUDV010000000
VERSION     DASUDV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930518"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /gene="ispF"
                     /locus_tag="VFV30_05785"
     CDS             <1..684
                     /gene="ispF"
                     /locus_tag="VFV30_05785"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX4847634.1"
                     /translation="RREELRRVQTPQAFRYPAILAAHRSWSGLPDAGDDAQVARAAGL
                     AVALVDGDEALHKLTFASDFGAAAPAVRIGTGYDVHRLAEGEELWLCGVKIDHPRGLA
                     GHSDADVAIHALVDAILGAIGSGDIGSHFPPSDPQWKGASSERFLTHAVKLAAEVGYR
                     IGNVDVTIICEAPKIGPHREAMRARLAELLGTGIEAVSVKATTTERLGFTGRGEGIAA
                     QAAATLLRD"
     gene            758..1249
                     /locus_tag="VFV30_05790"
     CDS             758..1249
                     /locus_tag="VFV30_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX4847635.1"
                     /translation="MNLLPEDLTELAQRVVAANREAGRMVAMAESCTGGLVSAALTEV
                     AGSSAVLDRGFVTYSNEAKHELLGVSNDIIDAFGAVSIACAWAMAQGALARSKADVAV
                     AITGIAGPDGGSEFKPVGTVVFARAVRGSDELVAEERFFAAESRGEVRRQAALVALEL
                     LLP"
     gene            complement(1210..1668)
                     /locus_tag="VFV30_05795"
     CDS             complement(1210..1668)
                     /locus_tag="VFV30_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX4847636.1"
                     /translation="MPGIREIRRLPYSAEQMFDLVADVARYGEFLPWVVATRVKSDSE
                     TEMVADMLVGFNALREKFTSRVIKDRPRRIEVIYVDGPMRDLDNVWEFRPTEHGCEIE
                     FHVDFTFRNAVFEKLAGQYFDRAFRKMVGAFEERAEKLYGSSSSSATSAA"
     gene            complement(1668..2594)
                     /gene="lipA"
                     /locus_tag="VFV30_05800"
     CDS             complement(1668..2594)
                     /gene="lipA"
                     /locus_tag="VFV30_05800"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX4847637.1"
                     /translation="MNDLSSPPKPERQRKPDWIRVKAPTSKGYGETRQLMRDLKLNTV
                     CEEAACPNIGECWTKKHATVMILGDVCTRACAFCNVKTGMPRAVDPLEPEHVAIAAAK
                     MGLEHIVITSVDRDDLPDGGASQFVKVIKALRANTPDTTIEILTPDFRGKMREAVEAI
                     VAAGPDVYNHNLETVPRLYPTIRPGARYYASLRLLEEVKAHDPLVFTKSGIMLGLGEE
                     RLEVHQVMDDMRSAGIDFITMGQYLQPTPKHTKVIEFVPPKTFDAYGAIARAKGFLQV
                     AASPLTRSSYHAGDDFKAMRAARQAQVARKGA"
     gene            2660..3313
                     /locus_tag="VFV30_05805"
     CDS             2660..3313
                     /locus_tag="VFV30_05805"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX4847638.1"
                     /translation="MPNRFSPALQHMIEGYQRFRKGAWTSNRERWTSLREGQKPEVMV
                     IACSDSRVDPAQIFDVDPGEIFVVRNVAALVPPFETTPGHHGVSAALEFAVQVLKVKK
                     IIVLGHGMCGGCKAALTKELHGTVPGEGGFIADWIALLDEARDEVAHDHGTEGRVAER
                     AMEQAGVKVSLANLRTFPCIREKERSGELRLRGAFFAISDGVLHLLDEEAGAFFPAP"
     gene            complement(3374..4759)
                     /locus_tag="VFV30_05810"
     CDS             complement(3374..4759)
                     /locus_tag="VFV30_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847639.1"
                     /translation="MVRAPFFARTALGMALALGVAAGGLSSVAVAKDKKPEAPKIQPT
                     KAFVPFYQAAAQGLDKASKRQDVIDARAAVTAAEGAYRSAQGKKARDEAKAAYDGAIA
                     KLIALLAPEYDLVEKALTAATAPEDKFYAGQLGLNLGNMAYDKRMQRRGILTVVESGK
                     LPAADAAKYQYFAGALSYDLKEYPAARTSLQAAIAGGYAGDEVTALLVDAYINDNMAV
                     EGLKVLEAASTGRSTPAPEAWLQKGVVVGYKGKFAEQANRFAYMLVDGYPTQSNWALS
                     IATVRDMSKFANQELIDLMRLMDRTNSFSEGRDYFDYIQSADARRLPGETLKIIDKGI
                     AAGKLEANDPAVKDQREIAAGRIAPDKASLAGLERDARAPNATAATAMAAGDAFLSYD
                     NPAKAVEMYTIALAKPGVDSPRVLTRLGIAQTDLGLYADAQATFAKVTGIRQPIAQLW
                     MAYAKSKAAGK"
     gene            5061..>5555
                     /locus_tag="VFV30_05815"
     CDS             5061..>5555
                     /locus_tag="VFV30_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4847640.1"
                     /translation="MSDETEIPESTPEPIGPLGEFQRVDIVDEMKTSYLDYAMSVIVS
                     RALPDVRDGLKPVHRRILFASQEGGFVAGRPYRKSAKIVGDVMGNYHPHGDSAIYDAL
                     ARMTQDWSMRLPLIDGQGNFGSMDPDPPASMRYTEARLAKVANSLLEDLDKDTVDFVD
                     NYDGS"
BASE COUNT          894 a         1843 c         1852 g          966 t
ORIGIN      
        1 cggcgcgagg agctgcgccg ggtgcagacc ccgcaggcgt tccgctatcc cgccattctg
       61 gccgcgcacc gcagctggag cggcctgccc gatgccggcg acgacgccca ggttgcccgc
      121 gcggcgggcc tggccgttgc cctggtcgat ggagacgaag ccttgcacaa gctgaccttt
      181 gccagcgatt tcggcgcggc cgccccggcg gtccggatcg gcaccggcta tgacgtccac
      241 cgcctggccg agggcgagga gctgtggctc tgcggggtga agattgacca cccgcgcggc
      301 cttgccgggc acagcgatgc cgacgtggcg atccacgcgc tggtcgacgc aatcctcggc
      361 gcgatcggca gcggcgatat cggcagccat ttcccgccca gcgacccgca gtggaagggc
      421 gccagttccg aacggttcct gacccacgcg gtcaaattgg cggccgaggt cggctaccgg
      481 atcggcaatg tcgacgtgac gatcatctgc gaagccccca agatcggccc ccaccgcgaa
      541 gcgatgcggg cccggctggc cgagctactg ggcactggga tcgaggcggt cagcgtcaag
      601 gcgactacca ccgagcgcct gggcttcacc ggccggggcg aagggatcgc ggcccaggcc
      661 gccgccaccc tgctgcgcga ctgaacgaat agcgctggat tttcccaccc gacgggacca
      721 aggggcttcg cgcgattcgc aacggacgga ttttcccttg aacctgcttc ctgaagacct
      781 gaccgaactg gcccagcgcg tggttgccgc caaccgcgag gcgggccgca tggtcgccat
      841 ggcggaaagc tgcaccggcg ggctcgtctc cgccgcgctg accgaagtgg ccgggtcctc
      901 ggccgtgctc gaccgcgggt tcgtgaccta ttcgaacgag gccaagcacg agctgctggg
      961 cgtttcaaac gacatcatcg atgcgttcgg cgccgtctcg atcgcttgtg cctgggccat
     1021 ggcccagggt gcactggccc gcagcaaggc cgatgtggcc gtggcgatca ccgggattgc
     1081 cggccccgat ggcggcagcg agttcaagcc ggtgggaacc gtggtctttg cccgcgccgt
     1141 gcggggcagc gacgagctgg tggccgagga gcgcttcttc gccgccgaat cgcggggcga
     1201 agtgcgccgt caggccgcgc tggtcgcgct ggagctgctg ctgccgtaaa gcttttcggc
     1261 gcgttcctcg aaagcgccga ccatcttgcg gaaggcccgg tcaaagtact ggccggcgag
     1321 cttttcaaac accgcgttgc ggaacgtgaa atcgacgtgg aattcgatct cgcagccgtg
     1381 ttcggtcggg cggaattccc agacgttgtc cagatcgcgc atcggcccgt cgacgtagat
     1441 cacctcgatc cggcggggcc ggtccttgat cacccgcgag gtgaactttt cccgcaaggc
     1501 gttgaagccg accagcatgt cggcgaccat ctcggtctcg ctgtcggact tgacccgggt
     1561 ggcgaccacc caaggcagga actcgccata gcgggccaca tcggcgacca gatcgaacat
     1621 ctgctcggcc gaatagggca gccggcggat ttcgcggatc cccggcatca ggcgcccttg
     1681 cgcgcgacct gcgcctgacg ggcggcgcgc atcgccttga aatcgtcgcc ggcgtgatag
     1741 ctggagcggg tcagcgggct ggccgcgacc tgcaggaaac ccttggcccg ggcaatcgcg
     1801 ccataggcat cgaaggtctt cggcggcacg aattcgatca ccttggtgtg cttgggcgtg
     1861 ggctgcaggt attggcccat ggtgatgaaa tcgatcccgg cgctgcgcat gtcgtccatc
     1921 acctggtgga cctccagacg ctcctcgccc agccccagca tgatcccgct cttggtgaag
     1981 accagcggat cgtgggcctt gacctcttcc agcaggcgca gcgaggcgta atagcgcgcg
     2041 ccggggcgga tcgtgggata gagccgcgga acggtctcga ggttgtggtt gtaaacgtcc
     2101 ggtcccgccg cgacgatcgc ctccaccgct tcgcgcatct tgccgcggaa atcgggggtc
     2161 aggatctcga tcgtggtatc gggggtgttg gcgcgcagcg ccttgatcac cttgacgaac
     2221 tggctggcac cgccgtccgg caggtcgtcc cggtcaaccg aggtgatgac gatgtgctcc
     2281 agccccatct ttgccgcggc gatggcgacg tgttcgggct ccagcggatc gaccgcgcgg
     2341 ggcatcccgg tcttcacgtt gcagaaggcg caggcccgcg tgcagacatc gcccaggatc
     2401 atgaccgtgg cgtgcttctt ggtccagcat tcgccgatgt tcgggcaggc ggcctcttcg
     2461 cacacggtgt tgagcttgag gtcccgcatc aactggcggg tctcgccata gcccttgctg
     2521 gtcggcgcct tgacccggat ccagtccggc ttgcgctggc gttcgggttt cggcggggag
     2581 gagaggtcgt tcatggctgc cacttaggcc gttcgttcgc cccttgccag aggcaattgt
     2641 gacgggcatt tggagcgcca tgcccaaccg cttctccccc gccctgcagc acatgatcga
     2701 gggctaccag cgcttccgca agggcgcctg gaccagcaac cgcgaacgct ggaccagcct
     2761 gcgcgaaggg cagaagcccg aagtgatggt catcgcctgc agcgacagcc gggtcgatcc
     2821 ggcccagatc ttcgatgtcg atccgggcga gatcttcgtg gtccgcaacg tcgccgcgct
     2881 ggttccgccg ttcgaaacca cgcccggcca ccacggcgtg tcggccgcgc tggaattcgc
     2941 ggtccaggtg ctgaaggtga agaagatcat cgtgctgggc cacggcatgt gcggcggctg
     3001 caaggcggcg ctgaccaagg aactgcacgg cacggtcccg ggcgaaggcg gcttcattgc
     3061 cgactggatc gccctgctgg acgaggcccg cgacgaagtg gcccacgatc acggcacaga
     3121 agggcgcgtg gccgagcggg cgatggagca ggccggggtc aaggtcagcc tggccaacct
     3181 gcgcaccttc ccctgcatcc gcgagaagga acgcagcggc gaattgcgcc tgcgcggcgc
     3241 ctttttcgcc atttcggacg gcgtgctgca cctgctggac gaagaggccg gcgcgttctt
     3301 tcccgccccc tgaccaacgg aaaagggcgg ccgtttccgg ccgcccttcc cgtgccttcc
     3361 agctgcgccg ggcttacttg ccggcggcct tgctcttggc ataggccatc cacagctgcg
     3421 cgatcggctg gcggatcccg gtaaccttgg cgaaggtcgc ctgggcatcg gcatagagcc
     3481 ccagatcggt ctgggcgatg ccgaggcggg tcagcacgcg cggcgaatcg acacccggct
     3541 tggccagcgc gatggtgtac atttcgaccg ccttggccgg gttgtcatag ctcaggaacg
     3601 catcgccagc ggccatcgcg gtcgccgcgg tggcgttggg cgcgcgggca tcacgctcca
     3661 gcccggccag cgaggccttg tccggagcga tacggcccgc ggcgatttcg cgctggtcct
     3721 tgaccgccgg atcgttcgct tcgagcttgc cggcggcaat gcccttgtcg atgatcttga
     3781 gcgtttcgcc cggcaggcgg cgcgcgtcgg ccgactggat gtagtcgaaa tagtcgcgcc
     3841 cttcggagaa gctgttggtc cggtccatca gccgcatcag gtcgatcagc tcctggttgg
     3901 caaacttgct catgtcgcga accgtggcga tcgacagggc ccagttgctc tgggtcggat
     3961 agccatcgac cagcatgtag gcaaagcggt tcgcttgctc cgcgaacttg cccttgtagc
     4021 caacgacgac gcccttctgc agccaggctt cgggggccgg agtggaccgc ccggtcgaag
     4081 ccgcttcaag gaccttcagg ccttcgacgg ccatgttgtc gttgatgtag gcatcgacca
     4141 gcagcgcggt cacttcgtca ccggcatagc cgcccgcgat cgccgcctgg agcgaggtcc
     4201 gcgcggccgg gtattccttc aggtcatagg acagcgcgcc ggcgaagtac tggtacttcg
     4261 cggcatcggc cgccggcagc ttgccgcttt ccaccacggt cagaatgccg cggcgctgca
     4321 tgcgcttgtc atacgccatg ttgccgaggt tgaggcccag ctggccagcg tagaacttgt
     4381 cttccggagc agtcgcggcg gtcagcgcct tttcgaccag atcgtattcc ggggcgagca
     4441 gcgcgatcag cttggcaatc gcaccgtcat aggcggcctt cgcttcatcc cgggccttct
     4501 tgccctgggc gctgcgatag gcgccttcgg ccgcggtcac cgcggcgcgg gcgtcgatca
     4561 cgtcctggcg cttgctggcc ttgtccagcc cctgcgcggc ggcctggtaa aacggcacga
     4621 aggccttggt cggctggatc ttgggggcct cgggcttctt gtccttggca accgccaccg
     4681 acgaaagacc accggcggca acacccagcg ccagcgccat gcccagggcg gtacgggcga
     4741 aaaacggggc acgaaccatt gaattcttct ccttggcaac tgccgcttgg agccgttctg
     4801 gcgccgcctg gcagggcaac ttcctcgtgt ccgccatagg cgttcccacg cccccttggc
     4861 aacagttagg cgcactaggg acagacggtt gaacggcaat tgaatgaagc cgcgcccgcc
     4921 tgttcagctt ggcttgccaa ggtgtggaaa aaacccggct tgaccccgct ttgcgcgcgc
     4981 ccgcgctggc cttcgcccgg cccatgccct agggccaagg gtgcgaattt ggtgccaaca
     5041 gaacaagtga tatttcccgc gtgagcgacg aaaccgaaat ccccgaatcc accccggaac
     5101 cgatcggccc gctgggcgaa tttcagcgcg tcgacatcgt cgacgaaatg aagaccagct
     5161 acctcgatta cgcgatgagc gtgatcgtca gccgcgccct gcccgatgtg cgcgacggtc
     5221 tgaagccggt tcaccgccgc attctgttcg ccagccagga aggcggcttc gtcgccggcc
     5281 ggccctatcg caagagcgcc aagatcgtcg gcgacgtgat gggcaactat cacccgcacg
     5341 gcgacagcgc gatctacgat gccctggccc gcatgaccca ggactggtcg atgcgactgc
     5401 cgctgatcga cggccagggc aacttcggct cgatggatcc cgatccgccg gcgtcgatgc
     5461 gctataccga agcgcggctg gccaaggtcg ccaattcgtt gctggaagac ctcgacaagg
     5521 acacggtcga tttcgtcgac aactatgacg gatcg
//
LOCUS       DASUDV010000271         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_759742,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000271 DASUDV010000000
VERSION     DASUDV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759742"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFV30_05820"
     CDS             complement(<1..881)
                     /locus_tag="VFV30_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847641.1"
                     /translation="MQFTPPTADQVLAIRVNAGIEELAGHDRFAAASPDMVEAIVEGI
                     GQFAAGEFAPLNRKGDLEGARLADGTVTLPDGFAAAYRAFVEQGWNAVAGDEAFGGQG
                     LPFTLAANVLENLGAANMAFSLLPMLTVGAIEALHHHGSEAQKATYLAKLVSGEWSGT
                     MNLTEPQAGSDVGALRTVATPRGDGTWLIKGTKIFITWGEHELAENIVHLVLARTPGA
                     PAGSRGISLFVVPKYHVNADGSPGARNDLRCAGLEHKLGIMASPTCVMSFGDNDQCVG
                     ELVGHENEGLKAMFTMM"
     gene            928..1875
                     /locus_tag="VFV30_05825"
     CDS             928..1875
                     /locus_tag="VFV30_05825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX4847642.1"
                     /translation="MTGKPDQLLIRAADSAGVAVAAARLRAGALVAVPTETVYGLAAR
                     ADSADAVAAIYRAKGRPDFNPLIVHVPDLDAAGALAEVGEAARLLARHFWPGALTLVL
                     PLKEGARIAPAVTAGLPTIALRCPAHPVMREVLEAAGLPLAAPSANRSGEVSPTRAEH
                     VAASLGAAAGLVLDGGACAAGIESTIVALRADGWQVLRPGPVTEAQISALLGPPLDAK
                     TEGRIEAPGQLASHYAPGKPVRLNAAAAAADEFLIGFGPVAGETSLSPSGDLAEAAAR
                     LYDALHRAAAAPQPRIAVAPIPAEGIGAAINDRLQRAAV"
     gene            complement(1872..2690)
                     /locus_tag="VFV30_05830"
     CDS             complement(1872..2690)
                     /locus_tag="VFV30_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX4847643.1"
                     /translation="MRFGKFAKALGPVIAVALAAGVAGCDGTVTFNGEEGKALSELDL
                     SGKAPEELVLAGPDEVRVTTGDKLAITVEGDPEVRDQVRFVLKDGSLAIHRKDKSVGS
                     GGKVAIINVTMPAPREVVMAGSGKITTAALASKAEVTVAGSGTVESTAVSGESLGLTI
                     AGSGNFRAAGAVKSLDMTIAGSGSAQMDTLKVDKADVTIAGSGSTAFSSDGEVEASIA
                     GSGNVTVKGRARCTVSSMGSGKLVCEGPVQESGKDAAEPPAPPQPPAPPEAPKS"
     gene            complement(2824..3360)
                     /locus_tag="VFV30_05835"
     CDS             complement(2824..3360)
                     /locus_tag="VFV30_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX4847644.1"
                     /translation="MVAKAHAFDVGDYVVYPKHGVGRVIELQEQEIAGMTLELYVLRF
                     EKERMTLRVPTNKVEAIGMRKLSSDKTLREALDTLKGKPKVKRTMWSRRAQEYEAKIN
                     SGDLVSIAEVTRDLFRADDQPEQSYSERQIFEAASSRLARELAAMEKTDEPAALKKIL
                     AILNEHAPKYYAEAAATV"
     gene            complement(3641..3979)
                     /gene="fdxA"
                     /locus_tag="VFV30_05840"
     CDS             complement(3641..3979)
                     /gene="fdxA"
                     /locus_tag="VFV30_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX4847645.1"
                     /translation="MTYVVTDACIKCKYTDCVEVCPVDCFYEGENMLVINPSECIDCG
                     VCEPECPAEAILPDTESGLEQWLELNAKYSAEWPNITQKKEPPADADEHKGEDGKFDK
                     YFSTEAGEGD"
     gene            complement(3994..4287)
                     /locus_tag="VFV30_05845"
     CDS             complement(3994..4287)
                     /locus_tag="VFV30_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX4847646.1"
                     /translation="MRIDKLLWFLRLARTRGAAQAVVAEGHIRLNGRRVERTAQAVAV
                     GDVMVLPLGRGVTVIELVALPGRRGPAEEARQCYRVLDGAPANPIAASDNNAP"
     gene            complement(4303..>4871)
                     /locus_tag="VFV30_05850"
     CDS             complement(4303..>4871)
                     /locus_tag="VFV30_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEX4847647.1"
                     /translation="ADSGLDRLAPEQRDRLRKLGVRVGALDLFVPDMLKPAALGAWRT
                     LLAARGAALPAPVPGMPPVVAVPGKTATPPGYRRLGKQALRIDLAEKLLRAAHERRVA
                     AGNRPFVIDPALAVSTGLATVGYAHLLRLGGFQANPARILPEGSFGPPQPARWRWRPL
                     RRETPGREAPPQPPRPGNAFAALAALVR"
BASE COUNT          678 a         1696 c         1675 g          822 t
ORIGIN      
        1 ttcatcatcg tgaacatggc cttcaggccc tcgttctcgt gcccgaccag ttcgcccacg
       61 cactggtcgt tgtcgccaaa gctcatcacg caggtcgggc tggccatgat ccccagcttg
      121 tgttccagcc cggcgcagcg caggtcgttg cgcgcgcccg gcgatccgtc ggcgttcacg
      181 tgatacttgg gcaccacgaa caggctgatc ccgcggctgc cggccggtgc gccgggggtg
      241 cgggccagga ccaggtggac gatgttctcg gccagctcgt gctcgcccca ggtgatgaag
      301 atcttggtgc ccttgatcag ccaggttccg tccccgcgcg gggtggcgac ggtgcgcagc
      361 gcaccgacgt ccgatccggc ctgcggctct gtcaggttca tcgtgcccga ccattcgccg
      421 ctgaccagct tggccagata ggtcgccttc tgggcctcgc tgccgtggtg gtgcagggcc
      481 tcgatcgcgc caacggtcag catcggcagc aggctgaagg ccatgttggc ggcgccgagg
      541 ttttccagca cgttggccgc cagggtaaag ggcaggccct gtccgccaaa ggcctcgtcg
      601 ccggccacgg cgttccagcc ctgctcgacg aaggcgcggt aggccgcggc aaagccgtcc
      661 ggaagggtca cggtcccgtc cgccagccgc gcgccttcca gatcgccctt gcggttcagc
      721 ggggcaaatt cgcccgccgc gaactggccg atcccttcga caatggcttc gaccatgtcg
      781 gggctggcgg cggcgaaacg gtcatggccg gccagctcct cgatcccggc gttgacccgg
      841 atggccagaa cctgatcggc ggtggggggc gtgaactgca tggcgctttt ccttggcttt
      901 gtcgccaacc gcctatagcg ggagcgcatg acgggcaagc cggaccagct cctgatccgc
      961 gccgcggact ccgccggggt ggccgtggcc gccgcgcggc tgcgcgccgg cgccctggtc
     1021 gcggtgccga ccgagacggt ctatggcctg gccgcccggg ccgacagcgc cgatgccgta
     1081 gcggcgatct accgcgccaa gggccggccg gacttcaacc cgctgatcgt ccatgtgccc
     1141 gatctggacg cggccggggc actggccgaa gtcggcgaag cggcccggct gctggcgcgg
     1201 catttctggc cgggcgcgct gaccctggtc ctgccgctga aagagggcgc gcgaatcgct
     1261 ccggcggtga ccgctggcct gccgaccatc gcgctgcgct gcccggccca tccggtgatg
     1321 cgcgaggtgc tggaggcagc cggcctgccg ttggccgcgc cttcggccaa ccgttcgggc
     1381 gaggtcagcc cgacccgggc cgagcatgtc gccgcttcgc tcggcgcggc ggcggggctg
     1441 gtgctggacg gcggggcctg cgcggccggc atcgaaagca cgatcgtggc gctgcgggcc
     1501 gatggctggc aggtgctgcg ccccggcccg gttaccgaag cgcagatttc cgcgctgctc
     1561 ggcccgccgc ttgacgccaa gacggagggc cggatcgaag cgcccggcca gctggccagc
     1621 cactatgcgc cgggcaagcc ggtccggctg aacgccgcag cggctgcggc ggacgagttc
     1681 ctgatcggct tcggaccggt ggcgggagag accagcctgt cgccctcggg cgatctcgcc
     1741 gaagcggcag cacggcttta cgatgcgctg caccgcgcgg ccgccgcgcc ccagccgcga
     1801 atcgccgtgg cgccgatccc cgcggaggga atcggcgccg cgatcaacga ccggctgcag
     1861 cgggccgcgg tttagctctt gggcgcttcc ggcggggccg ggggctgcgg cggagcgggc
     1921 ggttcagcgg cgtccttgcc gctttcctgg accgggcctt cgcagaccag cttgcccgaa
     1981 cccatcgagc tgaccgtgca gcgggcgcgg cccttgaccg tgacattgcc cgaaccggca
     2041 atgctggcct cgacttcgcc gtcagaggaa aaggcggtgc tgcccgatcc ggcgatcgtc
     2101 acatcggcct tgtcgacctt gagtgtgtcc atctgggccg agccgctgcc ggcaatggtc
     2161 atgtcgaggc tcttgaccgc gccggcggcg cggaaattgc ccgatccggc gatcgtcagg
     2221 cccagcgatt cgcccgaaac ggcggtgctt tcgaccgtgc ccgatccggc caccgtaact
     2281 tcggccttgc tggccagggc ggcggtggtg atcttgcccg atccggccat caccacttcg
     2341 cgcggggcgg gcatggtgac gttgatgatc gcgaccttgc cgcccgaacc aacgctcttg
     2401 tccttgcggt ggatcgccag gctgccgtcc ttcagcacga agcggacctg gtcgcggact
     2461 tcgggatcgc cttcgaccgt gatggccagc ttgtcgccgg tggtgacgcg cacttcgtcg
     2521 ggcccggcca ggaccagctc ttcgggcgcc ttgcccgaca ggtccagctc ggacagggcc
     2581 ttgccctctt ccccattgaa cgtgaccgtg ccgtcgcagc cggcaacacc ggcggccagc
     2641 gccacggcga tgaccggacc cagggccttg gcaaactttc caaacctcaa cttcatggcg
     2701 attctcccgc tatgtgtgtt ggggatataa tacagcaagg cgggcccttc aagggcacaa
     2761 acgaaaaggg ccgccccgtg gggggcagcc ctttcgctgt ccggggaaat ccggggcggc
     2821 cgatcagacg gtcgcggcgg cttccgcgta gtacttgggc gcgtgctcgt tgaggatcgc
     2881 gaggatcttc ttcagcgcgg ccggctcgtc ggtcttttcc atcgcggcca gctcacgcgc
     2941 aaggcgcgag gaggcggctt cgaagatctg ccgttccgaa taggactgtt ccggctgatc
     3001 atcggcgcgg aacaggtcgc gggtcacttc ggcaatcgag acgagatcgc ccgaattgat
     3061 cttggcttcg tattcctggg cgcggcgcga ccacatggtg cgcttgacct tgggcttgcc
     3121 cttcagcgtg tcgagcgctt cgcgcagggt cttgtcgctc gacagcttgc gcatcccgat
     3181 cgcttcgacc ttgttggtcg gcacgcgcag ggtcatgcgc tctttctcga accgcagcac
     3241 atagagttcc agagtcatcc cggcgatttc ctgctcttgc agttcgatca cccggccgac
     3301 cccgtgcttg ggatagacaa cgtagtcacc aacgtcgaag gcgtgggcct tggccaccat
     3361 gcaaaacgtc ctttcttgtg cgggatcggc accccccggt gcctgatgca gtcctgcggc
     3421 tgggcgaagc ggacggacaa tggggtcgcg acccggcccg atgcttccat caaccgcttt
     3481 cctgcatccc gcctttcacg atttgcggca gtttcccggg gcacagccca cctgccgtcg
     3541 cccgattata taacacaact gtaacataat tgccagcgcc gagaatcccg gcgctgacag
     3601 gcagttgttt cagtgctgga cgggccggtc cggcggggga tcaatcccct tcgccggcct
     3661 cggtggagaa gtacttgtcg aacttgccgt cttcaccctt gtgctcgtcg gcgtcggccg
     3721 gcggttcctt cttctgggtg atgttgggcc attcggccga gtacttggcg ttgagctcaa
     3781 gccactgctc cagcccgctt tcggtatcgg gcaggatcgc ttcggccggg cattccggct
     3841 cgcacacgcc gcaatcgatg cattcgctgg gattgatcac cagcatgttc tcgccctcgt
     3901 agaagcaatc gaccgggcag acctcgacgc agtcggtgta cttgcacttg atgcaggcgt
     3961 cggtgacgac ataggtcatg gcggctttgt cccctaaggt gcgttgttat cgcttgctgc
     4021 tatgggattt gcaggcgctc cgtcaagcac gcgatagcat tggcgcgctt cctcggccgg
     4081 tccgcgccgg ccgggcaggg ccacaagctc gatcacggtc accccgcggc ccagcggcag
     4141 gaccatcaca tcgcccaccg ccacggcttg cgcggtgcgc tccacccgcc gcccgttgag
     4201 ccggatgtgc ccctcggcca cgaccgcctg ggccgcgccg cgggtgcgcg ccaggcgcag
     4261 gaaccacagc aacttgtcga tccgcaccgc gccgccgccc gctcagcgca ccagggcggc
     4321 cagcgcggca aaggcgttgc cggggcgggg cggctggggc ggggcttcgc ggccgggcgt
     4381 ttcgcggcgc agcgggcgcc agcgccagcg cgccggttgc ggcgggccaa agctgccctc
     4441 gggcaggatc cgcgcggggt tggcctgaaa gccgccgagc cgcagcaggt gggcatagcc
     4501 gaccgtcgcc agcccggtcg acacggccag cgcgggatcg atcacgaagg gccggttgcc
     4561 cgccgccacc cgccgttcgt gcgcggcgcg cagcagcttt tcggcaagat cgatgcgcag
     4621 cgcctgcttg cccaggcggc ggtatccggg cggcgtggcg gtcttgcccg gcaccgcgac
     4681 caccggcggc atgcccggca ctggggccgg caaggccgcg ccgcgcgcgg ccagcagggt
     4741 gcgccaggcg cccagcgccg cgggcttcag catgtcgggc acgaacaggt cgagcgcgcc
     4801 gacccgcacg cccagcttgc gcagccggtc gcgctgttcg ggcgccagcc ggtccagccc
     4861 cgaatccgcc c
//
LOCUS       DASUDV010000272         9594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_406465,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000272 DASUDV010000000
VERSION     DASUDV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406465"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VFV30_05855"
     CDS             <1..149
                     /locus_tag="VFV30_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX4847648.1"
                     /translation="AGGLLLLIADCLCRILPLASELRLGIALSLLGAPFFLMLLLRMR
                     RGLA"
     gene            146..880
                     /locus_tag="VFV30_05860"
     CDS             146..880
                     /locus_tag="VFV30_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4847649.1"
                     /translation="MTIGAENLHLGQRLQGVSLTLNQGEVTAICGPNGAGKSSLLAAL
                     AGIQPLDGGTVRLDGDLLAGLDRADRARRIGLLPQQAELAWDLSVEVLAGLGRLPWKL
                     GPAEDAHAIDRALADTGLEALRHRPLSRLSGGERARAMLARVLAGEPCWLLADEPLAN
                     LDLAHQLSLLGHFRTMAQRGLGVVVVLHDLGQAMNHADRVVVLDGGRIAADGPPEQAL
                     SEAVIARIWNVQGRWLGEAGQRALVI"
     gene            complement(1016..3199)
                     /locus_tag="VFV30_05865"
     CDS             complement(1016..3199)
                     /locus_tag="VFV30_05865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX4847650.1"
                     /translation="MRKLTLVSMSALVLAGCAASSAEPAAAPVATATPVPAPYAPVIP
                     KGTGIFAEPSDLPFQAPDFSKIKESDYEPAIEQGITILLAEVDAIANNPEAPTFENTM
                     VALEKSGSMLTRVMYVFGALTSANTNDNLDAIDTRTAPKLAAMRDSITLNPKLFGRIK
                     TLYDNRASLNLAPDQLQVLELTYDGMVRNGALLSDADKAKLQDINGQLSSLQTDFGQK
                     LTKGTKDGALVVTDRKALAGLSAADIAAAEKGAADRGLPKGSYVLALQNTTQQPLLAA
                     LTTRSTRQALFERSWNRSERGDASDTRPLIVKIAALRAEKAKLLGFESFAAYQLSDQM
                     AKTPQAAISFLESFVPALGPAQKREAGELNALIKKEGGKFTVTPADWDHYAEKLRKQK
                     YALDQNEVKPYLEITNVLENGVFYAANQLFGLTFKKRTDIPVYHPDVTVYTVYDADGS
                     ELALFYFDPWKRDNKSGGAWMSNFVDQNGLTGTKPVVFNVSNYTKPAPGQPALVTWDD
                     ANTMFHEFGHALHGMLSNQRYPSIAGTNTARDFVEFPSQWFENFMTEPTVLANFAKHY
                     KTGEPMPAALVAKIDKASTFNQGYALGELLTAALLDLKWHSIQPGAAVGDVNAFENTA
                     LASLSEKGLQTTLVPPRYRTSYFRHIWGGGYAASYYAYSWTEMLNHDAYAWFKENGGM
                     TRKNGQHMRDTILSQGHARDYGVMYRNFAGRDPRVEPMLKARGLK"
     gene            complement(3293..5323)
                     /locus_tag="VFV30_05870"
     CDS             complement(3293..5323)
                     /locus_tag="VFV30_05870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX4847651.1"
                     /translation="MKSRFLIAGAALAVLAVPFATNPTLAQAVSAAAGKATIGAWGFD
                     LSGQDTSVKPGDDFFRFASGKWADRTEIPADKSLTGAFDDLRESTQAQLKDLITAAPK
                     TGQIGGFYASFMDEARLEKIGLTPLKADLAKVNAIKTKAQFSRYMGGTNGMFGAAVVD
                     FGVGADTANPDLNVLYLGQSGLGLPEREYYFADQFKKQREAYVAYIARTMKVLGVPNP
                     DQAAQTVMAFETGIAERSWKAAERRQIEKLNNPMTTAELMAFAPGIDWTQYFAGAKIP
                     AQKRMIVGENTAVKAISAFYAQTPLATLKLWQSFHIAHQASPYLNKAMVDSRFEFTKT
                     LSGVTANRPRWKRGVDLVGGALGELVGQDYVAKHFPASSKAKMEVLVANLKAAMAKRV
                     KANDWMAASTKAAALEKLEKMEVMVGYPDKWRSYKGLVILPGDLYGNVQRAGKFNADY
                     QMKDLGKPVDRKQWGMSPQTVNAYNGGLENKIVFPAGILQAPFFDPNADDAVNYGAIG
                     GVIGHEIIHGFDDQGRKIDSKGAVRDWWTPEDAKRFEEQAKIFGDQYAQFEVAPGAFI
                     NPKLSMGENIADLGGISVALEAYHASLGGKEAPVIDGLTGDQRFFLAWAQVWRTKMRE
                     AALRNQVNTGPHSPGMYRAFAPLRNVDGWYAAFNVEAGQKLYIAPEKRARIW"
     gene            complement(5466..6947)
                     /locus_tag="VFV30_05875"
     CDS             complement(5466..6947)
                     /locus_tag="VFV30_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847652.1"
                     /translation="MRLISHRSFASVPSLVAVGLLAAFAAPGAARAQDDPEGEAQASA
                     SLIATNTCNGVVNTVPPQATPDIDYKNDKWRAVPNSRLAYFAASHSGTMVAPGEALAL
                     VRKLDAPNPEDRPKELKPLRSLMYHLQNVLMTEELSGLRAFDKDGKPVTYTDSDHNRA
                     WLVRPEVVLRCKLPKKDEAAPQSAGNDGSGEPPVKKGKGWKIDPRLAGSVEALGASGE
                     ARKGAAAATVGYNRTRTFQADGSRTQTTEFSVKAFLGAAFDTGKPNEDLMVYGGYELK
                     RSRATPPPVLVPPATARDGDTEVVTLGAGISKLVPIGGSRNPYSSSLTFRFDGAYKFD
                     LVKDAERTEGKFSVSLYTPPRSVLGVCGFGGYTDFGNGFWSLCELSGGFSFNKVMKRG
                     TLVPSGKDSYAHAGGKASIALFYGDPSENSAFLSGEYLIMPRISGDQAEIKTIRRHKI
                     ALGYRWWSGTSHALEVKAELTDGINPDSYADENAVTIGFGILF"
     gene            complement(6938..7219)
                     /locus_tag="VFV30_05880"
     CDS             complement(6938..7219)
                     /locus_tag="VFV30_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847653.1"
                     /translation="MAHDHIDQANDAIAATVRKFRPSNIVGWNQTVPAKGLNMAGDAA
                     WMGFLGEIVAKYRELVGPCTCPDAETFADFKSKRLGLLRDALTEATQCA"
     gene            complement(7360..7716)
                     /locus_tag="VFV30_05885"
     CDS             complement(7360..7716)
                     /locus_tag="VFV30_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847654.1"
                     /translation="MKTTQMALAVLGSLALAATASAATGGNKKPGTTAPATAAAPTLS
                     PEEERAKANAEQAKAAAEQNAQNAANQQAYQEAVKARDAEIARQKAEHEAAVKKWEAD
                     VAACQAGDHSRCAKPN"
     gene            complement(7774..9462)
                     /locus_tag="VFV30_05890"
     CDS             complement(7774..9462)
                     /locus_tag="VFV30_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847655.1"
                     /translation="MIALRAKTAVSALALMALGTAAQPGATLTLPAGPGHSLALPLSL
                     TGTAQAATAKKPAPKKKTPPKKKAATKKTTKAAATKRVCTTKKVKGKAVRTCRTVKVA
                     PLAPPVVPVIVSTPVVQPVPVTPVPVVQIQPPPMPTPVPPPDPGPPPAAAFAWIDQAD
                     SLAGALGDAPPDFTFRYDGIDSWAWLTRGGEMLIVEPGRDGVVQYYFDRGANAPYLVR
                     GAFYSYAFDGPNLVQIYDNRGRLYVGELTYEQEYEARRLLARGQALLSASWRRRVWNG
                     DTAITWYDTLFTSGYYSWYGSNGWRGDWRDDWRRYPGWNDFHRDHRRNKPPRRLEEER
                     NNRKESAWNYARWRRDGGHGAPPPTGNPVIPNPAPSTLNPPPSRPAPPPAPAPAPVTP
                     QADPVPVAPPPPAPPPRRRPAWGTNPDGGTSPGSQAEPPRPAVPTVPESGASEPDRPR
                     PRMTRLLPPPPPPSEPPVQVQSVTPTTPPPAPAPVYQAPPPPPPPAPAYQAPPPPPPP
                     APVYVAPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPPALDRPAPITTGEP"
BASE COUNT         1397 a         3050 c         3427 g         1720 t
ORIGIN      
        1 tggccggcgg gctgctgctg ctgatcgccg attgcctgtg ccggatcctg ccgctggcca
       61 gcgagctgcg gctgggcatt gccctgtcgc tgctgggcgc gccgttcttc ctgatgctgc
      121 tgctgcgcat gcgccggggg ctggcatgac gattggcgcc gaaaacctgc acctgggcca
      181 gcgcctgcaa ggcgtgtcgt tgaccctgaa ccagggcgaa gtgaccgcga tctgtggtcc
      241 caacggcgcg ggcaagtcca gcctgctggc cgcgctggcg gggatccagc cgctggatgg
      301 cggcacggtg cggctcgacg gcgatctgct ggccggactg gaccgggccg accgcgcgcg
      361 ccggatcggc ctgctgccgc aacaggccga actggcctgg gacctgtcgg tcgaagtgct
      421 ggctggcctc ggccgcctgc cgtggaaact gggtccggcc gaggacgccc acgcgattga
      481 ccgggcgctg gccgataccg ggctggaggc gcttcgccac cggccgctgt cgcgcctgtc
      541 cggcggggag cgggcccggg cgatgctggc gcgggtcctg gccggagagc cgtgctggct
      601 gctggccgac gaaccgctgg ccaatctcga tctggcgcac cagctcagcc tgctgggcca
      661 tttccgcacc atggcgcagc gcgggctggg ggtggtggtg gtgctccacg atctgggcca
      721 ggcgatgaac cacgcagacc gggtggtggt gctggacggc gggcgcattg ccgccgacgg
      781 gccgcccgaa caggcgctga gcgaagcggt gatcgcccgg atctggaacg tgcaggggcg
      841 ctggctgggc gaggccggac agcgcgcctt ggtcatttag ttcctccccg gaacggggag
      901 ggggaccacg aagtggtgga ggggcacggg cgggtgatcc cgacagctca ggatcacccg
      961 cccgggcccc tccaccatcc ctgcgggatg gtccccctcc cccaaggggg aggatttact
     1021 tcaggccgcg ggccttcagc atcggttcga cccgggggtc gcggccggcg aagttgcggt
     1081 acatcacgcc gtaatcgcgc gcgtggccct gcgagaggat cgtgtcgcgc atgtgctggc
     1141 cgttcttgcg ggtcatgccg ccgttttcct tgaaccaggc ataggcatcg tggttcagca
     1201 tctcggtcca gctgtaggcg taatagctgg ccgcatagcc gccgccccag atgtggcgga
     1261 aatagctggt gcggtagcgc ggcgggacca gcgtggtctg caggcccttt tcgctgagcg
     1321 aggccagcgc ggtgttttca aaggcgttga cgtcgcccac cgcagcgccc ggctggatgc
     1381 tgtgccactt cagatcaagc agcgcggcgg tcagcagttc gcctagggca tagccctggt
     1441 tgaaggtgct ggccttgtcg atcttggcga ccagcgcggc cggcatcggc tcgccggtct
     1501 tgtagtgctt ggcgaagttg gccagcacgg tcggctcggt catgaaattc tcgaaccact
     1561 ggctcgggaa ttccacgaag tcgcgcgccg tgttggtgcc cgcgatcgag gggtagcgct
     1621 ggttcgagag catgccgtgc agggcatggc caaactcatg gaacatggtg ttggcatcgt
     1681 cccaggtgac cagcgccggc tggcccgggg cgggcttggt gtagttggac acgttgaaca
     1741 ccaccggctt ggtcccggtc agcccgttct ggtcgacgaa gttcgacatc cacgccccgc
     1801 ccgacttgtt gtcgcgcttc cacgggtcga aatagaacag cgccagttcg ctgccgtcgg
     1861 cgtcatagac ggtgtagacg gtcacgtccg ggtgatagac cggaatgtcg gtgcgcttct
     1921 tgaaggtcag cccgaacagc tggttggcgg catagaacac gccgttttcc agcacgttgg
     1981 tgatttccag gtagggcttc acctcgttct ggtcgagcgc gtacttctgc ttgcgcagct
     2041 tttcggcata gtgatcccaa tcggccgggg tgacggtgaa cttgccgcct tccttcttga
     2101 tcagggcgtt gagctcgccc gcttcgcgct tttgcgccgg gcccagggcg gggacgaagc
     2161 tttcgaggaa gctgatcgcg gcctgcggcg tcttggccat ctggtcggac agctgatagg
     2221 cggcgaagct ttcaaagccg agcagcttgg ccttttcggc ccgcagcgcg gcgatcttga
     2281 cgatcagcgg gcgggtgtcg ctggcatcgc cgcgttcgga gcggttccag ctccgctcga
     2341 acagcgcctg gcgggtgctg cgggtggtca gcgcggccag cagcggctgc tgggtggtgt
     2401 tctgcagcgc caggacatag ctgcccttgg gcaggccgcg gtcggccgcg cccttttcgg
     2461 cggcggcaat gtctgccgcg gaaaggccgg ccagcgcctt gcggtcggtc accaccagcg
     2521 cgccgtcctt ggtgcccttg gtcagcttct ggccgaaatc ggtctgcagg ctggaaagct
     2581 ggccgttgat gtcctgcagc ttggccttgt cggcatcgga cagcagcgcg ccgttgcgga
     2641 ccatgccgtc ataggtcagt tccagcacct gcagctggtc cggggccagg ttcaggctgg
     2701 cgcggttgtc atacagggtc ttgatccggc caaacagctt ggggttgagc gtgatcgaat
     2761 cccgcatggc ggccagcttg ggcgcggtgc gggtgtcgat tgcatccagg ttgtcgttgg
     2821 tgttggccga ggtcagcgcg ccgaacacgt acatcacccg ggtcagcatc gaacccgact
     2881 tttccagcgc gaccatggtg ttttcaaagg tcggcgcttc ggggttgttg gcgatcgcgt
     2941 cgacttcggc cagcaggatc gtgatcccct gttcgatcgc cggttcgtaa tcgctttcct
     3001 tgatcttcga gaagtcgggc gcctggaacg gcaggtccga aggctcggcg aagatcccgg
     3061 tgcccttggg gatgaccggg gcataggggg ccggaaccgg cgtggcggtg gccaccgggg
     3121 cggcggcggg ttcggcgctg gaggccgcgc aaccggccag gaccagggcg gacatcgaga
     3181 caagggtcag cttgcgcatg gaacaatcct cattcgggaa acgaatgggg gcagcctaac
     3241 gctgccccca tggagataca acgaacttgc accggcaggc gggagggccc gcttaccaga
     3301 tgcgggcgcg cttttcgggg gcgatgtaca gcttctggcc cgcttcgacg ttgaaggcgg
     3361 cgtaccagcc atcgacgttg cgcagcggag cgaaggcgcg gtacatgccc gggctgtgcg
     3421 gcccggtgtt gacctggttg cgcagcgcgg cttcgcgcat cttggtgcgc cacacctggg
     3481 cccaggccag gaagaagcgc tgatcgccgg tcagcccgtc gatcaccggg gcttccttgc
     3541 cgcccagcga ggcgtggtag gcctcgagcg cgaccgagat cccgccgagg tcggcgatgt
     3601 tttcgcccat cgacagcttc gggttgatga aggcgcccgg agcgacttcg aactgggcgt
     3661 actgatcgcc gaagatcttg gcctgctctt caaagcgctt ggcgtcttcc ggggtccacc
     3721 agtcgcgcac cgcgcccttg gaatcgatct tgcggccctg gtcgtcgaag ccgtggatga
     3781 tttcgtggcc gatcacgccg ccgatcgcgc cgtaattgac ggcatcgtcg gcgttgggat
     3841 cgaagaacgg cgcctgcagg atgccggccg ggaacacgat cttgttttcc aggccgccgt
     3901 tgtaggcgtt cacggtctgc gggctcatgc cccactgctt gcggtcaacc ggcttgccca
     3961 gatccttcat ctggtaatcg gcgttgaact tgcccgcgcg ctggacgttt ccgtaaaggt
     4021 cgcccggcag gatcaccagg cccttgtagg accgccactt gtcgggatag ccgaccatga
     4081 cttccatctt ctcgagcttt tcgagcgcgg cggccttggt cgaggcggcc atccagtcgt
     4141 tggccttgac tcgcttggcc atggcggcct tcaggttggc aaccaggact tccatcttgg
     4201 ccttggacga ggccgggaag tgcttggcga catagtcctg accaaccagc tcacccagcg
     4261 caccgccgac cagatcgacg ccgcgcttcc agcgcgggcg gttggcggtc acgcccgaca
     4321 gggtcttggt gaattcgaac cggctgtcga ccatcgcctt gttgagatag ggcgaggcct
     4381 ggtgcgcgat gtggaagctc tgccacagct tcagcgtggc gagcggggtc tgcgcgtaga
     4441 aggccgaaat cgccttcacc gcggtgtttt cgcccacgat catgcgcttc tgcgccggga
     4501 tcttggcgcc ggcgaagtac tgggtccagt cgatgccggg cgcaaaggcc atcagttcgg
     4561 ccgtggtcat cgggttgttg agcttttcga tctggcggcg ctcggcggcc ttccagctgc
     4621 gctcggcaat gccggtttca aaggccatca cggtctgcgc ggcctggtca ggattgggaa
     4681 cgcccagcac cttcatcgtg cgggcgatat aggcgacata ggcttcgcgc tgcttcttga
     4741 actggtcggc gaagtagtat tcgcgttccg gcaggcccag gcccgactgg cccaggtaga
     4801 gcacgttgag atccgggttg gcggtatccg caccgacccc gaaatcgacc acggcggcgc
     4861 cgaacatgcc gttggtgccg cccatgtagc ggctgaactg ggccttggtc ttgatcgcgt
     4921 tgaccttggc caggtccgcc ttcagcggcg tcaggccgat cttttccagc cgcgcctcgt
     4981 ccatgaagct ggcatagaag ccgccgatct ggccggtctt cggcgcggcg gtgatcaggt
     5041 ccttgagctg ggcctgggtg gattcgcgca gatcgtcgaa cgcgccggtc agcgacttgt
     5101 cggccgggat ctcggtccgg tcggcccact tgcccgaggc gaagcggaag aagtcgtcac
     5161 ccggcttgac cgaggtgtcc tggcccgaga ggtcaaagcc ccaggccccg atggtggcct
     5221 tgccggccgc ggcgctgacc gcctgggcca gggtcgggtt ggtggcaaac ggcaccgcca
     5281 gcacggccag cgccgcaccc gcgatcagaa aacgcgattt catcatggca attcccatcc
     5341 caagctttgc ggaccggatg cgccggcccc gaaaaagact gcccctgccc tcgcgcgcgg
     5401 gggcagggga gtcaatgtcg tttgccgaca ggcgtcgggc gttggtggaa ggggcggagc
     5461 ggcgcctaga acaggatgcc gaacccgatc gtcaccgcgt tctcgtcggc gtaggagtcc
     5521 ggattgatcc cgtcggtcag ctcggccttc acttcgaggg cgtgggacgt cccggaccac
     5581 cagcggtagc caagcgcaat cttgtgccgc cggatcgtct tgatctcggc ctgatcaccg
     5641 ctgatccggg gcataatcag atactccccg ctcaggaacg ccgagttttc gctcggatcg
     5701 ccgtagaaca aggcgatcga ggccttgccg ccggcgtggg cgtaactgtc tttgccgctc
     5761 ggcaccagcg tgccgcgctt catgaccttg ttgaagctga agcccccgga cagttcgcac
     5821 agcgaccaga acccgttgcc gaagtcggtg tagccgccaa aaccgcagac gcccaagacg
     5881 ctgcgcggcg gtgtgtacag gctgaccgag aacttgcctt cggtgcgttc ggcatccttc
     5941 accagatcga acttgtaggc cccgtcgaag cggaaggtca gcgaagagga gtagggattg
     6001 cgagacccac caatcgggac aagcttggat atccctgcgc ccaaagtgac gacttcggta
     6061 tcgccatcac gcgccgtcgc cggcgggacc aggaccggcg gcggcgttgc ccggctacgc
     6121 ttgagctcgt agccgccgta gaccatcaga tcctcgttag gcttgccggt gtcgaaggct
     6181 gcgcccagga aggccttgac cgagaactcc gtggtctggg tgcggctgcc atcggcctga
     6241 aaggtgcggg tgcggttgta gccgacggtg gctgctgctg cgcccttgcg ggcctcgcct
     6301 gaagctccca aagcctcgac cgaaccggcc agtcgcgggt cgattttcca gcctttgcct
     6361 ttcttcaccg gaggctctcc cgagccgtcg ttccccgcgc tctgaggagc ggcctcatcc
     6421 ttcttcggca gcttgcaacg caggaccact tccggccgga ccagccacgc gcggttgtga
     6481 tcggaatcgg tgtaggtcac cggctttcca tccttgtcga aggcgcgcag gccgctcagt
     6541 tcctcggtca tcagcacgtt ttgcaggtgg tacatgaggc tgcggagcgg cttcagctct
     6601 ttcggacggt cttccggatt gggcgcgtcc agcttgcgga caagcgcgag ggcttcaccc
     6661 ggagcaacca tcgttccgct gtggctggcc gcgaaatagg ccagccggga attcggaacc
     6721 gcgcgccatt tatcgttttt gtagtcgatg tccggcgttg cctgcggcgg gaccgtattg
     6781 accacgccgt tgcaggtgtt ggtggcgatc aggctggccg aggcctgcgc ctcgccttcc
     6841 ggatcgtctt gcgcccgcgc cgcacccggt gccgcgaaag ctgcgagcag tccgaccgca
     6901 accagtgaag gcacggaggc gaacgaacga tgagagatca ggcgcattgg gtggcctccg
     6961 tcagagcatc gcgcaacagg ccgaggcgct tggatttgaa atcggcgaac gtctccgcat
     7021 ccgggcaggt gcaagggccg acaagttcgc ggtatttcgc aacgatctct cccagaaagc
     7081 ccatccaggc cgcgtcgccg gccatgttca gcccctttgc aggcaccgtt tgattccatc
     7141 ccacaatgtt gctgggcctg aacttgcgaa ccgtcgccgc aatcgcgtcg ttggcctgat
     7201 cgatatggtc gtgcgccatg cgaatctccc ctgtttggca agcagcttag taaccgccgc
     7261 gccaaagcaa caccgtgatc cccgtcacgc acagcaaaac accgccgcgc cccgggtcgg
     7321 ggcaggcggt gtttgcgcaa ccggcggtct aggccgcggt cagttcggct tggcgcagcg
     7381 gctgtggtcg ccggcctggc aggcggcgac gtcggcttcc catttcttca ccgcggcctc
     7441 gtgctcggcc ttctggcggg cgatctcggc gtcgcgggcc ttgaccgctt cctgataggc
     7501 ctgctggttc gccgcgttct gggcgttctg ctcggccgcg gccttggcct gttcggcgtt
     7561 ggccttggcg cgctcttcct cgggcgagag ggtcggcgcg gccgcagtgg ccggggctgt
     7621 ggtaccgggc tttttgtttc cgccggttgc cgcgctggcg gtcgccgcca gggcgagcga
     7681 gcccagcacg gccagagcca tctgagtggt cttcatcgca ttcctccatt cgcggcgcag
     7741 gccgccgtcg cgagtccctg accgcagctt agcctatggc tcgccggttg taatgggcgc
     7801 cggacgatcc agcgccggtg gcggcggcgg aggtggcggc ggcggaggcg gaggcggcgg
     7861 aggcggaggc ggagggggcg gaggtggcgg aggcggaggc ggaggcggcg gcgggggcgg
     7921 cgcgacatag accggcgccg gcgggggcgg cggaggcggt gcctgatagg ctggagccgg
     7981 cggcggcggg ggtggcggag cctgatagac cggagccggc gcgggcggcg gcgtggtcgg
     8041 cgtcaccgac tggacctgca cgggcggctc gctcggcggc ggaggcggcg gcaacaggcg
     8101 ggtcatccgc gggcgcggac ggtccggttc gctcgcgccg ctttcgggca cggtcggaac
     8161 ggccgggcgc ggcggctcgg cctgggaacc ggggctggtc cccccatccg gattggtccc
     8221 ccaggccggg cggcggcgcg gcggcggggc cggcggcggc ggcgcgaccg gcacgggatc
     8281 ggcctgcggc gtaaccgggg ccggagccgg cgcgggcggc ggcgcgggcc gcgagggcgg
     8341 gggattgagc gtcgagggcg cggggttggg gatcaccggg ttgccggtcg gcggcggcgc
     8401 gccgtggccg ccatcgcgcc gccagcgggc atagttccac gccgattcct tgcggttgtt
     8461 gcgctcttcc tccagccgcc ggggcggctt gttgcggcgg tgatcgcggt ggaaatcgtt
     8521 ccagcccgga tagcggcgcc agtcgtcgcg ccagtccccg cgccagccgt tgctgccgta
     8581 ccaggaatag tagccgctgg tgaacagggt gtcgtaccag gtgatcgcgg tatcgccgtt
     8641 ccacacccgc cgccgccacg aggcggagag cagcgcctgc ccccgggcga gcagccgccg
     8701 ggcctcgtac tcctgctcat aggtcagctc gccgacatag agccggccgc ggttgtcata
     8761 gatctggacc aggttcggcc cgtcgaaggc gtagctgtag aacgcgccgc ggaccagata
     8821 gggggcgttg gcaccgcggt cgaaatagta ctggaccacg ccgtcgcggc ccggctcgac
     8881 aatcagcatc tcaccgccgc gggtcagcca ggcccagctg tcgatcccgt catagcggaa
     8941 agtgaagtcg ggcggggcat cgcccagcgc gccggccaga ctgtcagcct ggtcgatcca
     9001 ggcaaaggcc gcggccgggg gcgggcccgg gtccggcggg ggcacgggag tcggcatcgg
     9061 cggcggctgg atctggacca ccggaaccgg ggtgaccggc accggctgga ccaccggcgt
     9121 gctgacgatc accggcacca cgggcggcgc gagcggagca accttgaccg tgcggcaggt
     9181 ccgcaccgcc ttgcccttga ccttcttggt cgtgcagacc cgcttggtgg cggcggcctt
     9241 cgtcgtcttc ttggtcgcgg ccttcttctt gggcggggtc ttcttcttcg gtgccggctt
     9301 cttcgccgtg gcggcctggg ccgtgccggt cagcgacagc ggcagggcca ggctatggcc
     9361 cggtccggcc ggcagggtca gcgtggcccc gggctgggcc gccgtgccca gcgccatcag
     9421 cgccagggcc gaaacggcgg tcttcgcgcg caacgcaatc atacgaatct ccccaaaatc
     9481 cgaaccgggc gcgaccccct ttgaaggtgt tgtgccgcaa ggcaatttgc ccggagatga
     9541 acgaaccggg gggcggtgtg tcagacaagt gcgcgcccgg ctaggacaga cgcg
//
LOCUS       DASUDV010000273         6430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1378308,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000273 DASUDV010000000
VERSION     DASUDV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378308"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFV30_05895"
     CDS             complement(<1..673)
                     /locus_tag="VFV30_05895"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX4847656.1"
                     /translation="MASFTLPANSVIGRDGIHHKAEGATRARKFTVYRYDPDSGKNPH
                     YDVFEIDLDSCGPMVLDALIKMKSEMDPTLTFRRSCREGICGSCAMNINGRNGLACTT
                     AIEDLDGDIRITPLPAMEVIKDLVPDFTHFYAQYASIRPWLQTVSTTPSGKERLQSPE
                     QREKLDGLYECILCACCSTSCPSYWWNSDKFLGPAILLQAYRWLADSRDEMTGERLDE
                     LEDPFR"
     gene            complement(769..1305)
                     /locus_tag="VFV30_05900"
     CDS             complement(769..1305)
                     /locus_tag="VFV30_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4847657.1"
                     /translation="MTGNHQTAKSLPELTKKQRDVLALVADNRTSKEIAARLGISESA
                     VNQRIEMVRARLGGLPRGELARLYRQEFAPQDEVDPAAEPIWQKIHLPDSARNGQKGG
                     AEGISLPRSGGSQLVSRQSGDGSQLSAFFFEQDRTWLGREDRTTVLARRAAIVLIVAA
                     SLGIAMGVTQLLGPIGGG"
     gene            complement(1315..1758)
                     /locus_tag="VFV30_05905"
     CDS             complement(1315..1758)
                     /locus_tag="VFV30_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847658.1"
                     /translation="MVLFPFPPDGSSSALSIAFPLILLLTAAANLARAGSARRPAGRL
                     AFALAAHALTARFAPAASVTALLIDAALLAALSFDAVRRPSLLPVPIASLALIAVMAH
                     LLWQSGLLSSRFAYLTLVSCSWYGMVGLLGAGALLQAFRRRHVAQ"
     gene            complement(1770..2297)
                     /locus_tag="VFV30_05910"
     CDS             complement(1770..2297)
                     /locus_tag="VFV30_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4847659.1"
                     /translation="MPDILKQTALLDALTDKQREVIALVAEGMTSKEIARKIGISESA
                     VNQRIEVIRQRLDGMSRAQIARLYRQTSTLVITIPTSNSLTGKPIQLQSDYEVEQQSS
                     PEGVEDVLALANDEIDELQPSTFSPLPASWVALHGPSGPWIRAAAIVLIAVGLLAAAI
                     LVLAVARTVTDLAGH"
     gene            complement(2344..3237)
                     /locus_tag="VFV30_05915"
     CDS             complement(2344..3237)
                     /locus_tag="VFV30_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX4847660.1"
                     /translation="MQVYRTPDECFASLPDFPFAPHYLTLRGGLRLHYLDEGPRDAPV
                     VLLLHGEPTWCYLYRHMIGPIVAAGYRVVAPDLIGFGKSDKPLERAAYSYAGQVAWIR
                     EAIEALDLQRITLGCQDWGSLIGLRLAAEMPERFAGVALSNGGLPAGQEPPRAFAIWR
                     AFSRYSPVFPIGGIVKRGTRRPLSEAEVAAYNAPFPTRASKVATRVYPGLVPFAGNPA
                     VPDQLRAWEVFDRWEKPFLCCFSDSDPITRGGDAIWTERVPGTRGMAHRTLRGGHFIQ
                     EDDPAGFAAAIVEVARSDTPV"
     gene            3307..3939
                     /locus_tag="VFV30_05920"
     CDS             3307..3939
                     /locus_tag="VFV30_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4847661.1"
                     /translation="MGLGTWYDEHIVPRFIQCACSSPAIMKLRQKVVPLAAGKVFEIG
                     CGGGINQTFYDPAKVSSFSGMDPSAKGLEFTREAVARKGWQADVRQGFGEAIPFPDAS
                     FDCVVCTFTLCSVESQAQTLRELSRILRPGGRLLYAEHGRAPDPSVQKWQGRIEPVWK
                     KLAGGCHLTRPIGAPIADAGFHLNVLGQQYAPKTPRFAGWMEWGEAVKSG"
     gene            3944..5047
                     /locus_tag="VFV30_05925"
     CDS             3944..5047
                     /locus_tag="VFV30_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4847662.1"
                     /translation="MAKAKRKTLPKDFAELVRAGDAGRIRAVFDSCALDATGGYEKDT
                     ALAFVGLPEEVTRWLVEQSADVNARNSYQRTPLHRLAGHWQGEIALLLELGADPNAAD
                     QKGQTPLHFAARVGNEAAVRALLAAGADPGARNDAGQTPLQLALDQCHNAQIARVARV
                     AEVLLDATPAKPAGLLGRLLGRVPVNPASAPRLAEAVVRIGEQFEFHRAGFNPDTVDE
                     ADAGLQRLYELFGVPPVPRRVMHDGKARIDPGPGSWQDQHQALWELLVPSSGAAATVQ
                     GEVIRLSGRIDYEIAVNGGANWDEGYRKMADALLIHLASGTPLSEAEQAEAATAVRNV
                     RTKDDASLLCELAVAWVTRNPQPVALGRVAYNR"
     gene            complement(5131..>6430)
                     /locus_tag="VFV30_05930"
     CDS             complement(5131..>6430)
                     /locus_tag="VFV30_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX4847663.1"
                     /translation="QLRLRDMAGLVVIDFIDMEYNSNIRKVEKAMKDALKNDRARIQV
                     GRISSFGLMEMSRQRLRTGVLEATTRSCPHCDGTGLVRTASSAGLSALRLIEDEAAKG
                     RGSVISLYASTEAAVYLLNAKRADLAEIEERYGVRVEVLPEGENEGAKMRVASSGPRP
                     EFVPRFEPIAIEEDDDDVIEEDEDDFDGDAEERGGDDGDGRGKRRRKRRRRGGRGRDG
                     EGQPRDGDDADRDGEDRDEGDEDGERDERDDRAQGEDGEGPRKKRRRGRRRRGGRGRN
                     EGEGLNEAGEATDENGEGAAGAEAEAPAEAPVADEAPAKPKRTRKKKADAEAEAVTEA
                     EPAPAEEPAAVAEEAPAKPKRTRKKKVEAEAEAEAAAPELAAEEPAAEAPAKPKRSRK
                     KAVEPAAEVATAEATEAPAETPEEAPAAPRRGWWQRTFGE"
BASE COUNT         1031 a         2082 c         2216 g         1101 t
ORIGIN      
        1 ggcggaaagg atcttccagc tcgtcgagcc gctcgccggt catctcgtcg cggctgtcag
       61 ccagccagcg ataggcctga agcaggatcg ccgggcccag gaacttgtcg ctgttccacc
      121 agtaggaggg gcagctggtc gagcagcagg cgcacaggat gcactcgtag aggccgtcca
      181 gcttctcgcg ctgttcgggg ctctgcaggc gttccttgcc cgacggcgtg gtcgacacgg
      241 tctgcagcca gggacggatc gaggcgtact gggcgtagaa gtgggtgaag tccggaacca
      301 ggtccttgat cacttccatc gccggcagcg gggtgatgcg gatgtcgcca tccagatcct
      361 cgatcgcggt ggtgcaggcc aggccgttgc ggccgttgat gttcatcgcg caggacccgc
      421 agatcccttc gcggcaggaa cggcggaagg tcagcgtcgg atccatttcc gacttcatct
      481 tgatcagcgc gtccagcacc atcgggccgc aggaatcgag atcgatctcg aacacgtcgt
      541 agtgcgggtt cttgccgctg tccggatcat agcggtagac ggtgaacttg cgggcgcggg
      601 tggcgccttc ggccttgtga tggatgccgt cgcggccgat cacgctgttg gcgggaagag
      661 tgaagctcgc catttcgatc cctgttcgct ggtgggcaac gcccgtgggc ttggggcctc
      721 tctagcgatt cagcgcgccg gggcaaggtg gcccaacagg aaatttgttc agccgccgcc
      781 gatcgggccc agcaactggg taacccccat cgcaatgccg agcgaggcag cgacgatcag
      841 aacgatcgcg gcccggcgcg caaggaccgt tgtgcgatcc tcgcggccga gccaggtgcg
      901 atcttgctcg aagaagaatg cggaaagttg cgacccatcc cctgactgcc gcgatacgag
      961 ttgcgaccct ccggaccgag gcaaggagat accctccgcg ccgcccttct gtccgtttcg
     1021 ggccgaatcc ggaaggtgaa ttttctgcca tatcggctct gccgcagggt caacctcgtc
     1081 ctggggggcg aattcctgcc ggtaaagccg ggccagttcg ccgcgcggca ggccgcccag
     1141 gcgggcgcgg accatctcga tccgctggtt cacggcgctt tcggaaatgc ccagccgggc
     1201 ggcgatttcc ttgctggtcc ggttgtccgc caccagggcc agcacatcgc gctgcttctt
     1261 cgtcagctcg gggagcgact tggcagtctg gtgattgccg gtcatgacgg gtccctattg
     1321 cgcgacgtgc ctgcgccgaa acgcctggag cagtgcgcct gcccctagca ggccaaccat
     1381 gccataccat gaacaggaca caagcgtcag ataggcaaag cggcttgaaa gcaagccgga
     1441 ctgccacagc agatgggcca tgaccgcgat cagggcgagg gacgcgatgg gcaccggcag
     1501 cagggatggg cggcgcactg catcaaatga caaggccgcc agcagcgccg catcgatcag
     1561 cagcgcggtc acgctggctg ccggggcgaa ccgggcggtc agggcatggg ccgccaaggc
     1621 gaaggccagt ctgccggccg gccggcgggc cgacccagcc cgcgcaagat tggccgctgc
     1681 cgtcagcaga aggatgagcg ggaacgcaat cgataaggcg ctggatgagc cgtcaggcgg
     1741 gaaggggaac agcaccatgg cgctgaccgt cagtgtccgg ccaggtcagt gacggttctg
     1801 gccactgcca ggaccaggat cgcggcggcc agcaggccga ccgcaatcag cactatcgcg
     1861 gcggcgcgga tccatggccc ggaaggacca tggagcgcga cccaggaggc gggaaggggg
     1921 gaaaaggtgg agggttgcaa ctcgtcgatt tcatcgttgg ccagcgccag gacgtcctcg
     1981 acaccctccg gggacgattg ctgctccacc tcgtagtcgc tctgaagctg gattggctta
     2041 cctgtaagcg aattactggt gggaatcgtg atcaccaaag tgctcgtctg gcggtacaga
     2101 cgcgcgatct gggcgcgcga cataccgtcc agccgctgcc ggatcacttc gatccgctgg
     2161 ttcaccgcgc tctcggaaat acctattttt cttgcaattt ctttgctcgt catgccctcg
     2221 gcaaccagcg cgatcacttc gcgttgcttg tccgtcaggg catcgagcag ggctgtctgt
     2281 ttgagaatat ccggcatcgc ccggccggtt tacccgctta ccgggctggt agcaaatagg
     2341 tcattatacc ggggtgtcgg aacgggccac ctcgacaatc gcggcggcga atccggcggg
     2401 atcgtcttcc tggatgaagt ggccgccgcg cagggtgcgg tgggccatgc cgcgggtgcc
     2461 gggaacccgc tcggtccaga tcgcatcgcc gccgcgggtg atcggatcgc tatccgaaaa
     2521 gcagcacagg aacggcttct cccagcggtc gaacacctcc caggcgcgga gctgatccgg
     2581 caccgcggga ttgccggcaa aggggaccag gccgggatag acccgggtgg cgaccttgct
     2641 ggcgcgggtc ggaaaggggg cgttgtaggc cgcaacctcg gcctcgctga gcgggcggcg
     2701 ggtaccccgc ttcacgatcc cgccaatcgg gaacaccggg ctgtagcggg aaaaggcgcg
     2761 ccagatcgca aaggcgcggg gcggttcctg cccggcggga aggccgccgt tggacagggc
     2821 gaccccggca aaccgctccg gcatttcggc cgccagccga agcccgatca gcgagcccca
     2881 gtcctggcag cccagcgtga ttcgctgcag gtccagcgcc tcgatcgcct cgcggatcca
     2941 ggcgacctgc ccggcatagg aataggccgc gcgctccagc ggcttgtcgc tcttcccgaa
     3001 gccgatcagg tctggggcca ccacgcggta accggccgcc acgatcgggc cgatcatgtg
     3061 ccggtagagg taacaccagg tcggctcgcc gtggagcagc aggacgaccg gcgcgtcgcg
     3121 cggcccctca tcaaggtagt gcagccgcag cccgccgcgc agcgtcaggt aatgcggcgc
     3181 gaaggggaaa tcgggcaagc tggcaaagca ttcgtccgga gtgcgataga cctgcatctt
     3241 gaacctttcc ctcggcgtgg tttgtgccat catggcccat gatcaacggc aaaggaagga
     3301 ccggctgtgg gactgggcac atggtacgac gagcatattg tcccgcgctt catccagtgc
     3361 gcctgctcgt ctccggcgat catgaaactg cggcagaagg tggttccgct ggccgccggc
     3421 aaggtgttcg agatcggctg cggcgggggc atcaaccaga ccttctatga cccggccaag
     3481 gtcagcagct tttcgggcat ggacccgtcg gccaaggggc tggagttcac ccgcgaggcg
     3541 gtggcgcgca agggttggca ggccgatgtc cgccagggct ttggcgaggc gatcccgttt
     3601 cccgatgcca gcttcgactg cgtggtctgc acattcaccc tctgctcggt ggaaagccag
     3661 gcacagaccc tgcgcgagtt gagccgcatc ctgcgtcccg gtggccgcct gctctatgcc
     3721 gaacacggcc gggcgcccga tccttcggtc cagaagtggc aggggcggat tgagccggtc
     3781 tggaagaagc tggccggggg ctgccacctg acccgcccga tcggcgcgcc gattgccgat
     3841 gccggatttc acctcaacgt gctgggccag caatacgccc ccaagacccc gcgctttgcc
     3901 gggtggatgg aatggggcga ggccgtgaag tcgggttagg ttgatggcca aggccaagcg
     3961 caagaccctg cccaaggatt tcgccgaact ggtccgcgcc ggcgatgccg gccggatccg
     4021 tgccgtgttc gacagctgtg cgcttgatgc gaccggcggc tatgaaaagg atacggcgct
     4081 ggcctttgtc gggttgccgg aagaggtcac ccgctggctg gtggagcaga gcgccgatgt
     4141 gaacgcccgc aacagctatc agcgcacgcc gctgcatcga cttgccggcc attggcaggg
     4201 ggagattgcg ttgctgctgg aactgggcgc ggatccgaat gccgcggacc agaagggcca
     4261 gaccccgctg catttcgccg cccgggtagg aaatgaggcg gctgtccgcg ctctgcttgc
     4321 cgccggcgcc gatcctggag cgcgcaatga tgcaggccag acgccgctgc aactggcgct
     4381 tgatcaatgc cacaacgccc agattgccag agtggcccgg gtcgcggagg tcttgctgga
     4441 cgcaacaccg gccaagccgg cgggtctgct gggcaggctg ctcggccgag tgccggtcaa
     4501 tccagcctcc gccccccgcc tggcagaggc ggtcgtacgg attggcgagc agttcgaatt
     4561 tcacagggcc ggcttcaacc ccgacacggt ggacgaggca gatgccgggc tgcagcggct
     4621 ctatgaactg ttcggcgtgc ccccggtgcc gcgccgggtg atgcacgatg gcaaggcccg
     4681 gatcgatccc gggccgggca gctggcagga tcagcaccag gccctgtggg agctgctggt
     4741 tccatcaagc ggagcggctg cgactgttca gggcgaagtg atccggctgt ccgggcggat
     4801 cgactacgag attgcggtga acggcggagc gaactgggac gagggctatc gcaagatggc
     4861 cgatgccctg ctgattcatc tggccagcgg aacgccgttg agcgaagcgg agcaggccga
     4921 ggccgcaact gcagttcgaa acgtgcgcac gaaggacgat gcaagcctgc tgtgcgaact
     4981 ggcggtggcc tgggtgacgc gcaatccgca gccggtggcg ctgggccgcg tcgcctacaa
     5041 ccgctgaagc ccgcagcgat cgcgctcatc gggcaatgaa aaccccgccc cgtcggtttg
     5101 acggggcggg gtgtttcatg tgtacgaagc ttattccccg aaggtccgct gccaccagcc
     5161 gcggcgcggg gcggcggggg cttcctcggg cgtttcggcc ggggcttcgg tagcctctgc
     5221 ggtcgccact tcggcagccg gttcgacggc cttcttgcgg ctgcgcttgg gcttggccgg
     5281 agcttcggcg gccggttctt ccgcagccag ttccggagcg gccgcttcgg cttcagcttc
     5341 agcctcaacc ttcttcttgc gggtgcgctt cggcttggcc ggggcttcct cggcaaccgc
     5401 ggcgggttcc tcggccggag ccggctcggc ctcggtcaca gcttcagcct cggcgtcggc
     5461 cttcttcttg cgggtccgct tgggcttggc cggggcctcg tccgccaccg gggcttcagc
     5521 cggagcctct gcctcggcgc cagccgcgcc ttcgccgttc tcgtccgtgg cttcgccggc
     5581 ttcgttcagg ccttcgccct cgttccgccc gcgaccgccg cggcggcgac gaccccggcg
     5641 gcgcttcttg cgcggacctt cgccgtcttc gccctgggcg cggtcgtcac gctcgtcgcg
     5701 ctcgccgtct tcgtcgccct cgtcacggtc ctcgccatcg cgatcggcgt cgtcgccgtc
     5761 acggggctgg ccttcgccat cgcggccacg gccgccacgc cggcggcgct tgcggcggcg
     5821 cttgccacgg ccatcgccat cgtcgccgcc gcgctcttcg gcgtcgccgt cgaaatcgtc
     5881 ctcgtcctct tcgatcacgt cgtcatcgtc ttcctcgatg gcgatcggtt cgaagcgggg
     5941 cacgaattcc ggacgcggac cggacgaggc gacgcgcatc ttggcgcctt cgttctcgcc
     6001 ttccggcagg acttcgaccc ggacgccata gcgttcctcg atctcggcga gatcggcgcg
     6061 cttggcgttg agcaggtaaa ccgccgcttc ggtgctggca taaagcgaga tcaccgaacc
     6121 gcggcccttg gcggcctcgt cctcgatcag gcgcagcgcc gaaaggcccg cgctggaagc
     6181 ggtgcggacc aggccggtgc cgtcgcagtg cgggcacgag cgggtggtgg cttcgagcac
     6241 gccggtgcgc aggcgctggc ggctcatttc catcaggccg aagctggaaa tccggccgac
     6301 ctggatgcgg gcgcggtcgt tcttcagcgc atccttcatg gccttttcga ccttacggat
     6361 gttggagttg tactccatgt cgatgaagtc gatcacgacc agcccggcca tgtcgcgcag
     6421 gcgcagctgg
//
LOCUS       DASUDV010000274         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_362436,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000274 DASUDV010000000
VERSION     DASUDV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362436"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFV30_05935"
     CDS             <1..881
                     /locus_tag="VFV30_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4847664.1"
                     /translation="VGSGDLKERFLTPLVEGRARSAFFMTEPAELNGAGADPSMMITT
                     CRKDGNHWVINGKKCFITGAEGAKVGIVMAKSEDPDSAGGACMFLVDLPDPAIQITGV
                     PNTIDSSMPGSHAEITIDNLRVPADQMLGDAGEGFKYAQIRLSPARLSHCMRWHGCCQ
                     RAHEIATDYANRRHAFGKALIDHEGVGFMLAENLIDLKQAELMIAWCAEVLDSGSLGT
                     AESSMTKVAVSEALMRIADRCVQVMGGMGVTDHTIVEQVFREVRAFRIYDGPTEVHKW
                     SLAKKIKRDWKAALPG"
     gene            complement(882..1487)
                     /locus_tag="VFV30_05940"
     CDS             complement(882..1487)
                     /locus_tag="VFV30_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX4847665.1"
                     /translation="MTEIEFLYDFGGPNSWFVHRAIPAVEAQGKVRFRYSPVLLGGLF
                     KATGNKPPLMTYGHVAAKVAYDRLEMDRFMARHGITGFQFNPHFPVNTLLAMRGAIAV
                     ERLGCGPAYREAMYTALWQDGLKLDDPEVWAGAVAAAGLDASALGALVQDDAVKAELV
                     SNTEAAVARGAFGVPTFFLGGEMWFGKDRLRDVVEAAGGTL"
     gene            complement(1487..2209)
                     /locus_tag="VFV30_05945"
     CDS             complement(1487..2209)
                     /locus_tag="VFV30_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847666.1"
                     /translation="MTAPQQSVAVIGAGDFIGAAIVRKFAAEGFAVHAGRRNGDKLAP
                     LLGGAVTGRSLDAREPDDVAAFLAEAEAIAPLALVVFNVGANVQFSLTETTDRVFRKV
                     WEMACFAGFVSVREAARIMAPRGAGKIFVTGATAGLRGNPGYAAFAAAKAGLRAMAQS
                     AARELWPQGIHVAHLVIDAGVDTEWVRARVTERIGAEALAAQPDLLMPPEAVAEAYLA
                     LFGQPKAAWTFEQEIRPYGETW"
     gene            complement(2206..2652)
                     /locus_tag="VFV30_05950"
     CDS             complement(2206..2652)
                     /locus_tag="VFV30_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX4847667.1"
                     /translation="MSNASIWWGGKRPPLEPMTALGENALPGHIGIEFVDCGDDWIRA
                     RMPVDHRTQQPFQRLHGGASVALAETIGSVAASYCVDRSTHVAVGMEINANHIRPAYS
                     GWVYATARPEALGRTTQVWTIRIEDEAGKLVCISRFTVAVIPAERK"
BASE COUNT          477 a          959 c          917 g          410 t
ORIGIN      
        1 aggtcggcag cggcgatctg aaggagcggt tcctgacccc gctggtggaa ggccgcgccc
       61 gttctgcctt cttcatgaca gaaccggccg agctgaacgg cgcgggcgcc gatccttcga
      121 tgatgatcac cacctgccgc aaggacggca atcactgggt gatcaacggc aagaagtgct
      181 tcatcaccgg agccgagggc gcgaaggtcg ggatcgtgat ggccaagtcc gaagacccgg
      241 acagcgcagg cggggcctgc atgttcctcg tcgatctgcc tgaccccgcg atccagatta
      301 ccggcgtgcc caataccatc gacagctcga tgcccggcag ccatgccgaa atcaccatcg
      361 acaacctgcg agtgcccgcc gaccagatgc tgggcgatgc gggcgagggg ttcaaatatg
      421 cccagatccg cctgtccccg gcgcggcttt cgcactgcat gcgctggcac ggctgctgcc
      481 agcgcgcgca cgagatcgcg accgactatg ccaaccgccg ccacgccttc ggcaaggccc
      541 tgatcgatca cgaaggggtc ggcttcatgc tggcggagaa cctgatcgac ctgaagcagg
      601 ccgagctgat gatcgcctgg tgcgccgagg tgctggacag cggctcgctc ggcacggcgg
      661 aaagctcgat gaccaaggtg gccgtgtccg aagcgctgat gcggatcgcg gaccgctgcg
      721 tccaggtgat gggcggcatg ggggtgaccg accacaccat cgtcgaacag gtgttccgcg
      781 aagtgcgcgc gttccggatc tatgacggcc cgaccgaggt ccacaagtgg agcctggcca
      841 agaagatcaa gcgcgactgg aaggccgcgc tgccgggctg atcagagcgt tccgccggcc
      901 gcttccacca cgtcacgcag gcggtccttg ccgaaccaca tctcgccgcc gaggaagaag
      961 gtcggcacgc caaaggcccc gcgcgccacg gcggcttcgg tgttggaaac cagctcggcc
     1021 ttgaccgcgt cgtcctgaac cagcgcgccc agcgccgagg catcgagccc ggccgccgca
     1081 accgcaccag cccagacctc cggatcgtcc agcttcagcc cgtcctgcca cagcgcggtg
     1141 tacatcgcct cgcggtaggc cggtccgcag cccagccgct cgaccgcgat cgcgccgcgc
     1201 attgccagca gggtgttgac cgggaaatgc gggttgaact ggaacccggt gataccgtgg
     1261 cgggccatga agcggtccat ctccagccgg tcataggcca ccttggccgc gacatggcca
     1321 taggtcatca gcggcggctt gttgccggtg gccttgaaca ggccgcccag cagcaccggg
     1381 gaatagcgga agcgcacctt cccctgggcc tcgaccgcgg gaatggcgcg gtggacgaac
     1441 cacgaattgg gcccgccgaa gtcgtagagg aattcgatct ccgtcatcac catgtctccc
     1501 cgtacgggcg gatttcctgc tcaaaggtcc aggccgcttt cggctggcca aagagcgcga
     1561 ggtaggcctc ggccacggcc tctggcggca tcagcagatc gggctgggcg gcaagggcct
     1621 cggccccgat ccgctcggtc acgcgggcgc ggacccattc ggtatcgacc ccggcatcga
     1681 tcaccaggtg ggccacgtgg atcccctggg gccacagctc gcgcgccgcg ctttgcgcca
     1741 tggcgcgcag gccggccttg gcggcggcaa aggcggcata gcccggattg ccgcgcagcc
     1801 cggcggtggc accggtgacg aagatcttgc cggccccgcg cggcgccatg atccgcgccg
     1861 cttcccgcac ggacacgaac ccggcaaagc aggccatttc ccagaccttg cggaacaccc
     1921 ggtcggtcgt ttccgtcagc gaaaactgca cgttggcgcc cacgttgaac accaccagcg
     1981 ccagcggggc aatggcctcg gcctcggcca gaaaggccgc gacatcgtcc ggctcgcgcg
     2041 cgtcgaggct gcggccggtg accgcgccgc ccagcagcgg ggcgagcttg tccccattgc
     2101 ggcgcccggc gtggacggca aagccttcgg cggcgaactt gcggacaatc gccgcgccga
     2161 tgaaatcgcc cgcgccgatc accgcgaccg attgctgcgg cgcggtcact tgcgctccgc
     2221 cgggatcacc gcaacggtga agcgcgaaat gcagaccagc ttgccggcct cgtcctcgat
     2281 ccggatcgtc cagacctggg tggtccgacc cagcgcctcg ggccgggcgg tggcatagac
     2341 ccagccggaa taggccgggc ggatgtggtt ggcgttgatt tccatcccca cggcgacatg
     2401 ggtgctgcgg tcgacgcaat agctggccgc gaccgaaccg atcgtttccg ccagcgcgac
     2461 cgaagcccca ccgtgaagcc gctggaacgg ctgctgggtg cggtgatcga ccggcatccg
     2521 cgcccggatc cagtcatcgc cgcagtcgac gaattcgatg ccgatatggc ccggcagggc
     2581 gttctcgccc agcgcggtca tgggttccag gggcggacgc tttccgcccc accagatcga
     2641 tgcgttgctc attgcaactg tctgacgcaa agcggcggcg ctggcaatcc ggcaccgccg
     2701 cctgtccggc caagaaaaac tgtcaggccg cttcggtgac cttcttgccg gcacggaccc
     2761 cgt
//
LOCUS       DASUDV010000275         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1002799,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000275 DASUDV010000000
VERSION     DASUDV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002799"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFV30_05955"
     CDS             <1..743
                     /locus_tag="VFV30_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847668.1"
                     /translation="LAGVFLVLPSFAVGILAPPPEPPAGADGDKLMAILSTYYSSAWP
                     WLLGMAVCQVIGTLAMLALYTDRTRPTVAEALQLALRGALPVILAQILAGMAFAGVAL
                     ALISLAAMTGSQAAATLMVTLSLIALIYVIVRISLVAPVVMVDGERNPIAALKRSWAL
                     TRGNAGRLLLFYALLLIGFGIVILLVGGLADLLLRLALGMELGTALGTLVSAALQAVM
                     AIFFMAVYAATHRQLARPEAPTAVIPIE"
     gene            complement(747..1373)
                     /locus_tag="VFV30_05960"
     CDS             complement(747..1373)
                     /locus_tag="VFV30_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4847669.1"
                     /translation="MTSTQLARKPLSARIKQRVMRAMGPWGVFLQGFIEHPVMVGSII
                     PSSRFTIARMLGPVKWDECKLFVEYGPGVGTFCRPVLERLPRDGRLIVIDTNPLYIDY
                     LKATIADSRFVPVLGSAADVEDIVHAHGFDHADYVLSGLPFSTLPDGVGPAIAAATHR
                     VLRPGGAFLVYQFSAKARDFMARHFNRIDADFEWLNVLPCKLFWGWKE"
     gene            1473..2825
                     /locus_tag="VFV30_05965"
     CDS             1473..2825
                     /locus_tag="VFV30_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847670.1"
                     /translation="MTTPATPPPGPTDTVTASLPRSRVALLFTVMLVTAAGNTAMQSV
                     MPSIGTALKVHDFWISLAYTWSALLWMLCAPFWARRSDRRGRKAMMALGLTGFISSFG
                     LCGMALWLGLNGWLGALGTLLLFAACRSLYGGFGSAAPPAVQAYVASRTSRAERTQAL
                     SLISSSFGLGTVLGPALAPLLIVPGLGLVSPFFAFTLIAVIVLVALRLRLPDDAPAYA
                     ARGEVLAAPFSANSDSRVRGEGEEADDGALPAQVPDLKWTDRRIRPWLIAGLLGGHAQ
                     AMVMGIVGFLILDRLGLRATPMAGAGPTGLVLMSGAIATLLAQWGIIPLWKLGPRASA
                     LWGVSLAALGVALLAVAQDLHAITIGTAIACLGFGLFRPGFTAGASLAVSRAEQGQSA
                     GIVASVNGAAYILAPAVGVWLYGHSSWIGFAVIELLCLAVLALGIKGLGRDEDLIERR
                     "
     gene            complement(2822..3166)
                     /locus_tag="VFV30_05970"
     CDS             complement(2822..3166)
                     /locus_tag="VFV30_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX4847671.1"
                     /translation="MKATIWHNPACGTSRKTLAILQETPGVDVTVVEYLVNPPSAEKL
                     AQLYRDAGITPQQGLRLRGTDAEERGLPSADAATVLAAMAAEPKLIERPLVETEKGVR
                     LCRPQDKVLEIL"
BASE COUNT          467 a         1103 c         1119 g          611 t
ORIGIN      
        1 cgctggccgg ggtcttcctc gtcctgccga gcttcgccgt cgggattctc gccccgccgc
       61 ccgagccgcc agcaggggcg gatggcgaca agctgatggc gatcctgtcc acctattaca
      121 gcagcgcctg gccgtggctg ctgggcatgg cggtgtgcca ggtaatcggc accctggcga
      181 tgctggcact ctataccgac cggacccgcc cgactgtcgc cgaggcgctg caactggcgc
      241 tacgcggcgc cctgccggtg atcctggcgc agattctggc gggcatggcc ttcgccggag
      301 ttgcgctggc gctgatctcg ctcgccgcga tgaccggatc gcaggctgcc gcaacgctga
      361 tggtcacgct gagcctgatc gcgctgatct atgtgatcgt ccgcatctcg ctggtcgcgc
      421 ccgtcgtgat ggtcgatggc gagcgcaatc cgatcgcggc gctcaagcgt tcctgggctc
      481 tcacccgggg caatgccgga cggctgctgc tgttctatgc tctgctgctg atcggcttcg
      541 ggatcgtaat tctgctggtc ggtggcctgg ccgatctgct gctgcgactg gcgctgggaa
      601 tggaactcgg cacggcactg ggcacgctgg tgtcggcagc gctgcaggcg gtgatggcga
      661 tattcttcat ggcggtctat gccgccacgc accgccagct ggcccgcccg gaagcgccga
      721 cagcggtcat cccgatcgaa taagggctac tctttccagc cccagaacag cttgcagggc
      781 agcacgttca gccactcaaa atcggcatcg atccggttga agtgccgcgc catgaaatcg
      841 cgcgccttgg cgctgaactg atagaccaga aaggccccgc ccggccgcag cacgcggtgg
      901 gtggccgcgg cgattgctgg accaacgcca tcgggcaggg tcgagaacgg caggcccgac
      961 aggacatagt cggcatgatc gaagccgtgg gcgtggacga tatcttccac gtccgccgcc
     1021 gatcccagca cgggcacgaa ccggctgtcg gcaatcgtgg ccttcaggta atcgatatag
     1081 agcgggttgg tatcgatcac gatcagccgc ccgtcgcgcg gcaaccgctc aagcaccggg
     1141 cggcagaacg tgccgacacc ggggccatat tcgacgaaca gcttgcactc gtcccacttc
     1201 accggcccga gcatccgcgc aatcgtgaag cgcgacgacg ggatgatcga gccgaccatc
     1261 accggatgct cgataaagcc ctgcaggaag accccccacg gtcccatcgc gcgcatgacg
     1321 cgctgcttga tccgggcgga gagcggcttg cgggccagct gggtcgaagt catgcggggg
     1381 gctggttcct gtattaggca gctatagcgc cgctttcaca aatttgcccg accattgcaa
     1441 ggcggttgcg accaacatag ggggagaagg cgatgaccac ccccgcaacg cccccgcccg
     1501 gtcccactga cacggtcacg gccagcctgc cgcgcagccg cgtggcgctg ttgttcacgg
     1561 tcatgctggt cacggcagcg ggcaatacgg cgatgcaatc ggtcatgccc tcgatcggca
     1621 cggcgctgaa ggtccacgat ttctggatca gcctggccta tacctggtcg gcgctgctgt
     1681 ggatgctctg cgccccgttc tgggcgcggc gcagcgaccg gcgcgggcgc aaggcgatga
     1741 tggcgctggg gctcaccggg ttcatctcca gcttcggcct gtgcggcatg gcgctgtggc
     1801 tgggcctcaa tggctggctt ggcgcgctgg gcaccttgct gctgtttgcc gcctgccgct
     1861 cgctctacgg cgggttcggc tcggccgcgc cgccggcggt tcaggcctat gtcgccagcc
     1921 gcaccagccg ggccgagcgg acccaggcgc tttcgctgat ctcctccagc ttcggcctgg
     1981 gcacggtgct cggcccggcg ctcgccccgc tgctgatcgt gccggggctg ggcctggtca
     2041 gcccgttctt tgccttcacc ctgatcgccg tgatcgtgct ggtcgcgctg cgcttgcgcc
     2101 tgcccgacga tgccccggcc tatgccgcgc gcggcgaagt gctggccgcg ccgttcagcg
     2161 ccaattcgga ctcgcgcgtg cgcggcgagg gcgaagaggc tgacgacggc gcgttgccgg
     2221 cgcaagtgcc cgacctcaag tggaccgacc gccggatccg cccctggctg atcgccggcc
     2281 tgctcggcgg ccatgctcag gcgatggtca tgggcattgt cggcttcctg attctcgacc
     2341 ggctgggcct gcgcgccaca ccgatggccg gcgccgggcc gaccgggctt gtgctgatgt
     2401 ccggcgcgat cgccacgctg ctggcgcagt gggggatcat tccgttgtgg aagctgggtc
     2461 cgcgggcttc ggcgctgtgg ggcgtcagtc tggcagcgct gggcgtggcg ctgctggccg
     2521 tggcgcagga cctccacgcc atcaccatcg gcacggccat cgcctgcctg ggcttcggcc
     2581 tgttccgccc gggctttacg gctggcgcca gcctggcggt cagccgcgcc gagcagggcc
     2641 agtcggctgg gatcgtcgcc tcggtcaatg gcgcggccta tatcctcgcc ccggcggtcg
     2701 gggtctggct gtatggccac tcgtcatgga tcggctttgc cgtgatcgaa ctgctctgcc
     2761 tcgcggtgct ggcccttggc atcaagggcc tgggccgcga cgaggacctg atcgagcggc
     2821 gttagaggat ctccagcacc ttgtcctgcg gccggcacag ccgcacgccc ttctcggttt
     2881 cgaccagcgg gcgctcgatc agcttgggct ctgccgccat cgccgccagc acggtagccg
     2941 catcggctga aggcaggccg cgctcttccg catcggtgcc gcgcaggcgc aatccctgct
     3001 gcggggtgat cccggcatcg cgatagagct gggcgagctt ttcggcgctg ggcgggttca
     3061 ccagatattc gaccacggtg acatcgactc cgggggtctc ctgcaggatc gccagcgttt
     3121 tgcgcgaggt gccgcaggcc gggttgtgcc agatcgttgc cttcatggtt ggggtccttt
     3181 gcattcgtcc cggctccata gcgaatacga tgagccgagc aatttgatcc ttgtcaatgc
     3241 gagcgattcg cattagcggc attctcgtta ttgcaaatca ttcgcaagat ggggatctga
//
LOCUS       DASUDV010000276         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_251575,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000276 DASUDV010000000
VERSION     DASUDV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251575"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFV30_05975"
     CDS             complement(<1..441)
                     /locus_tag="VFV30_05975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEX4847672.1"
                     /translation="MTELSVLDLVPVREGGTLAQAYADAAELARCAEAAGYKRFWVAE
                     HHAMDGVAGAATSVVLAHIGQATQTIRIGAGGIMLPNHNPFVIAEQFGTLDALFPGRI
                     DLGLGRAPGADMRIAQALRRDLARVSEYFPQDVVELRALLSGQPE"
     gene            complement(438..1295)
                     /locus_tag="VFV30_05980"
     CDS             complement(438..1295)
                     /locus_tag="VFV30_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847673.1"
                     /translation="MAEPDDMNLTFLLPLFAQVGPAGAMEHAPLEIPRAKKAAAPVIE
                     GRLARCIELAHSAPLDAIRETDKWLAEAKGSARADPGECKGLALTRLERWAQAAATFA
                     AAHDDTPVFEQALRARRGAMAGNAALAAGQAEAALAALDSAHGEARRAKETALAGGIA
                     LDRARTLVALGRIEEAASALAEARGALAGDAQTWLLSATLSRRLGKLPEAQGQIEQAA
                     ALDPRDPAIGLEAGVIAMLAGREEAARKSWEAVVAMAPDSAEATTARGYLDQLGLDPS
                     DKPAQSSRR"
     gene            complement(1295..2026)
                     /locus_tag="VFV30_05985"
     CDS             complement(1295..2026)
                     /locus_tag="VFV30_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847674.1"
                     /translation="MTPARFHSLPDGRRIAFRHSPGSGPALVFLPGYMSDMAGGKATA
                     VFDWARAQGRECLLLDYSGCGESEGHFADGSLSRWRDEVLALIEAQCRGPVVLIGSSM
                     GGWLMLLCALADPARVHALVGIAAAPDFTQWGTSAADKARLAAGETVYEDNPYGPEPT
                     PTHARFWADGQAQLLLDQPIPIDCPVRLLHGQADADVPWEISLRLAQALRSADVQVHL
                     IKDGDHRLSRDEDIALLLRTLAALP"
     gene            2193..>2801
                     /locus_tag="VFV30_05990"
     CDS             2193..>2801
                     /locus_tag="VFV30_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847675.1"
                     /translation="MTLPQPEAAPITHTTRRSLFKLAALGLAGASTPLLAQDAARGFT
                     HGVASGEPGANQVLLWTRYVGSGDVRLTFEVAEDEQFTRAIASGECTASPGSDHCAKA
                     WARGLAPGKWYFYRFIAPSGEMSCVGRTRTLPVGPTARFRIAVFSCSNYGFGHFNAYA
                     HAAEANEFDLAVHLGDYFYEYERGEYPTLRQVIDQRLVGLEET"
BASE COUNT          428 a          973 c          976 g          424 t
ORIGIN      
        1 ttcgggctgg ccgctcagca gcgcgcgcag ctcgaccaca tcctgcggaa aatattccga
       61 aacccgcgcc agatcgcggc gcagggcctg ggcgatccgc atgtccgcgc ccggcgcccg
      121 gcccaggccc agatcgatcc ggcccgggaa cagcgcatcg agcgtgccga actgctcggc
      181 gatcacgaag ggattgtggt tgggcagcat gatcccgccc gcgccgatcc ggatggtctg
      241 cgtggcctgg ccgatatggg ccagcaccac cgaggttgcg gcgccggcga ccccgtccat
      301 cgcatggtgt tcggccaccc agaagcgctt gtagccggcc gcttcggcgc agcgggcaag
      361 ttcggcggca tcggcatagg cctgagccaa agtgccgcct tcgcggaccg ggacaagatc
      421 gaggactgaa agttcggtca ccgccgggat gattgggccg gcttgtccga ggggtcaagc
      481 ccgagctgat cgagatagcc gcgcgcggtc gtggcctcgg cgctgtcggg ggccatggcg
      541 accaccgcct cccagctctt gcgcgcggcc tcctcgcgcc cggccagcat cgcgatcacc
      601 ccggcctcga gcccgatcgc cggatcgcgc ggatctaggg cggcggcctg ctcgatctgg
      661 ccctgggctt cgggcaattt gcccagccgg cgcgaaagcg tggccgagag cagccaggtc
      721 tgggcatcgc cggccagcgc gccgcgtgcc tcggcgagcg ccgaagcggc ttcctcgatc
      781 cggccgagcg ccaccagcgt tcgcgcccga tcaagcgcga tgcccccggc cagcgcggtt
      841 tccttggccc gccgggcctc accatgggcc gaatcgagcg cggccagcgc ggcttcggcc
      901 tggcccgccg ccagcgcggc attgccggcc atggccccgc gccgtgcccg cagcgcctgt
      961 tcgaagaccg gggtgtcatc atgcgccgcg gcaaaggtgg cggccgcctg cgcccagcgt
     1021 tcgagccggg tcagcgccag ccccttgcac tcgcccggat cggcgcgggc cgatcccttc
     1081 gcttcggcga gccacttgtc ggtttccctg atcgcatcga gcggggcgct gtgcgccagt
     1141 tcgatgcagc gcgccagcct gccttcgatc accggggcgg cagccttttt ggcgcgggga
     1201 atttccagcg gggcatgttc catcgcgccg gccgggccga cctgggcaaa caggggaaga
     1261 agaaaagtca ggttcatgtc gtccggttcc gccatcaggg cagtgcggcc agggtccgca
     1321 gcagcagcgc gatatcctca tcgcggctga ggcgatgatc gccgtccttg atcaggtgca
     1381 cctgcacatc ggctgaacgc agcgcctgcg cgagccgcag cgatatctcc cagggaacgt
     1441 cggcatcggc ctggccgtgg agcaggcgga ccgggcaatc gatggggatt ggttggtcca
     1501 gcagcagctg cgcctggcca tcggcccaga accgggcgtg ggtcggggta ggttcggggc
     1561 cataggggtt gtcttcatag accgtttcgc ccgcggcgag ccgggccttg tcggcagcgc
     1621 tggtacccca ttgggtaaaa tcgggcgcag cggcgatccc gaccagcgcg tgaacccgcg
     1681 cgggatcggc cagcgcgcac agcagcatca gccagccgcc catcgagcta ccgatcagca
     1741 ccaccgggcc gcggcactgg gcctcgatca gcgccagcac ctcgtcgcgc cagcggctga
     1801 ggctcccatc ggcaaagtgg ccttcgcttt ccccgcagcc cgaatagtca agcagcaggc
     1861 attcgcgccc ctgcgccctg gcccagtcga acaccgcggt ggccttgccg cccgccatgt
     1921 cgctcatgta gccgggcagg aacaccagcg ccgggccgga gccgggacta tggcgaaagg
     1981 cgatgcggcg gccatcgggc agggagtgga atcgggctgg cgtcatgcct tcaggacatg
     2041 ccgcaagcgg cccggccagt cacgctggaa gatcgccgcg atcggcctgc gctgcgatat
     2101 cggtcaaatc gatttgcctg gtcgcggggc ctgtccttgc tcggtcaaaa aagcgcgcta
     2161 gtctggccgg cgaacccaac aggaaggccg cgatgaccct gccccagccc gaagcagcgc
     2221 cgatcaccca cactacccgc cgcagcctgt tcaagctggc cgcgctgggc cttgccgggg
     2281 cgagcacgcc gctgctggcg caggatgcgg cgcggggctt cacccacggg gtggcgagcg
     2341 gcgagccggg ggcgaaccag gtgttgctgt ggacgcgcta tgtaggctcg ggcgatgtgc
     2401 ggctgacctt cgaggtggcc gaggacgagc agttcacccg cgccatcgcc agcggcgagt
     2461 gcacggcatc gcccggcagc gatcattgcg ccaaggcctg ggcgcgcgga ctggcgccgg
     2521 gcaagtggta cttctaccgc ttcatcgcgc cgtcgggcga aatgtcgtgc gtgggccgca
     2581 cccgcaccct gccggtaggg ccgaccgcgc ggtttcggat cgcggtgttt tcctgttcga
     2641 actatggctt tggccatttc aacgcctatg cccatgccgc cgaggccaac gaattcgacc
     2701 tggcggtgca tctgggcgac tacttctacg aatacgagcg cggcgaatat cccacgctgc
     2761 gccaggtgat cgaccagcga ttggtgggcc ttgaggagac c
//
LOCUS       DASUDV010000277         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1776167,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000277 DASUDV010000000
VERSION     DASUDV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776167"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="fliI"
                     /locus_tag="VFV30_05995"
     CDS             complement(<1..341)
                     /gene="fliI"
                     /locus_tag="VFV30_05995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012317.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEX4847676.1"
                     /translation="MLKLFDPLLEELGGLGIDLSPGRYGLVTACDGGLIEVSGLAAPV
                     GALCRLAHGRDQSLTAEVIGFRNGRSLMMLLGDTVMLRPGARVRPEGRPGMLSVGEAF
                     LGRAVDGEGQP"
     gene            complement(335..910)
                     /locus_tag="VFV30_06000"
     CDS             complement(335..910)
                     /locus_tag="VFV30_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX4847677.1"
                     /translation="MRPGPSLPLGALAASSTFISDPRFSDGTPPAEPVRADPASDAWG
                     EGYAAGRAEAEAQAALAAQQSQADQARIELSLAHLDADLQEGLRHKLHATIVALCEAT
                     LAPLALDREALARRVAKASEMLARADDDKLLRLHPEDLALVADRLPAGLATEPDPGLE
                     RGALRIETSTGGVEDGPAHWRRAIGEALAQC"
     gene            complement(1160..2188)
                     /gene="fliG"
                     /locus_tag="VFV30_06005"
     CDS             complement(1160..2188)
                     /gene="fliG"
                     /locus_tag="VFV30_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX4847678.1"
                     /translation="MSEELQQVAGLGEAERAAVMIMLLEEEQAAAILGELGPDELQLL
                     GEKMCALGDIGPEQITQAISGFVQNSEHSGISARGRTGHVRSLMNRAVGEVKAESLMQ
                     RILPMGEEAGPAIELARWLTPQALIPLIKDEHPQAIAVLLVQLDPEIAAEVLHSLPPE
                     SQTAVVHRVATLGPVSAEAIAMLEAVLDQKIAQCHGKAALTMGGPREAANIINNAGKA
                     AEKRIMPEINKLDKALAREIEAEMFKFEHLFALSPQDMGALLRDVESEVLIDALKGTA
                     EEDREAFFRAMSSRAADGVKDEIAARGRLKMAEVLEAQKAIVTIARRLAGEGTIQFGV
                     GGGDDDYV"
     gene            complement(2185..>3270)
                     /locus_tag="VFV30_06010"
     CDS             complement(2185..>3270)
                     /locus_tag="VFV30_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HEX4847679.1"
                     /translation="VRDNAPPSASVMVRMARGRQLGDSQVAAVVNLVAGSVPGMSPDA
                     VRVVDQHGRLLSAKGSAENDRLELQGRMEDKLRAQIDQLLTPMLGANMFSSEIQIELD
                     LDQVTSARESYDKDGVVRSESQAQSQSTGGAQAGGVPGVLSNTPPPASAVRAGAPQPG
                     MVPGAPAGAGSGESSATRTYELGREVSVANAAPGRVKRLSVAVAISQAALKKAKPEEI
                     EQIKALVTAAVGADPQRGDQVQVLVRPFEPISDEAPPFYEAPWFAMVVRSAVALIGVL
                     LVLLLAVKPLIGVLRRRGENATGGDGTPGTEPSAAQSGNPAATGTDPAMLGRQVSLAQ
                     RIVEEKPEDALVALRQMLAQSRPETAA"
BASE COUNT          511 a         1132 c         1044 g          583 t
ORIGIN      
        1 atcggctggc cttcgccatc gaccgcgcgg cccagaaagg cttcgcccac gctcagcatg
       61 ccgggtcgcc cttcgggccg aacccgcgcc cccgggcgca gcatcaccgt atcgcccaga
      121 agcatcatca ggcttcggcc gttgcgaaag ccgatcacct cggcggtcag cgactgatcg
      181 cgtccgtgtg ccagccggca cagcgccccg accggcgcgg caaggccgga aacctcgatc
      241 agcccgccat cgcaggcggt gacaaggccg tagcgaccgg gcgatagatc gatgccaagt
      301 ccgcccagtt cctccagcag ggggtcgaac agcttcagca ctgggccagc gcctcgccaa
      361 tcgcgcggcg ccagtgcgcc ggaccatcct cgaccccgcc ggtcgaggtt tcgatcctga
      421 gtgccccgcg ttcaaggccg gggtcgggtt cggttgcaag accggctggg aggcgatcgg
      481 caaccagggc cagatcttca ggatgaagcc gcagcagctt gtcgtcatcg gcccgtgcga
      541 gcatctcgct ggctttggca acgcggcgcg caagggcttc gcgatcgagc gccagcgggg
      601 ccagggtcgc ctcgcacagc gcaactattg tggcgtgcag cttgtggcgc aggccttcct
      661 gaagatcggc atcgagatgc gcgagcgaca gctcgatccg tgcctggtcc gcctggcttt
      721 gctgcgctgc cagcgcggcc tgggcctctg cttcggcacg cccggcggca tagccctcgc
      781 cccaggcatc ggaagcggga tcggcgcgaa ccggttctgc tggcggcgtg ccatccgaaa
      841 agcgcggatc gctgatgaag gtgctcgatg cggccagggc tccaagcggc aggctagggc
      901 caggacgcac tatccgcacc ttcgaggttt gaggcaaaat ggctcagtgc taggaaggaa
      961 ggcgatggaa tatgtcgcta tttcaaggct tcctgacgca gcaatgggcc attttgacca
     1021 aatcgcgcag cgacggcgca atggcttcca tctcgaacgc aaattccctt gtaaaggcaa
     1081 ccagccttcc ccgcgggaat tgccgtccga tctgttcgcc attccgccgc ctcgaaggtg
     1141 cggatagtgg gtcctgaccc tagacataat cgtcgtcccc gccgccaacc ccgaactgga
     1201 tcgtgccttc gccggccagg cgccgggcga tggtgacgat cgccttctgc gcctcgagca
     1261 cttcggccat cttgaggcgg ccgcgcgcgg cgatctcatc cttgacgccg tcggcggcgc
     1321 ggctcgacat ggcgcggaag aaggcttcgc ggtcttcttc ggccgtgccc ttcagcgcgt
     1381 cgatcaggac ctcgctttcc acatcgcgca agagggcgcc catatcctgc gggctgagtg
     1441 cgaagagatg ctcgaacttg aacatctcgg cctcgatctc gcgcgcgagc gctttgtcga
     1501 gcttgttgat ctcgggcatg atccgctttt cggcagcctt gccggcattg ttgatgatgt
     1561 tcgccgcctc gcgcgggccg cccatggtga gggcagcttt gccgtggcac tgggcgatct
     1621 tctgatcgag caccgcctcg agcatggcga tcgcttcggc ggaaaccggg ccaagcgttg
     1681 ccacccggtg caccaccgcg gtctggcttt ccggcggcag cgaatgcagc acttcggcgg
     1741 cgatttcggg atcgagttgc acgagcagca ccgcgatcgc ctggggatgt tcatccttga
     1801 tcagcgggat cagtgcctgc ggggtcagcc agcgcgcgag ttcgatcgcg gggccggctt
     1861 cctcgcccat cggcaggatc cgctgcatca ggctttcagc ctttacctcg cccaccgcgc
     1921 ggttcatcaa cgatcggaca tggcccgtgc ggccgcgcgc gctgatgccg ctgtgttcgg
     1981 aattctgaac gaagccggaa atcgcctggg tgatctgttc ggggccgata tcgcccagcg
     2041 cgcacatctt ttcgcccagc agctggagct cgtcaggccc cagttcgccc aggatcgccg
     2101 ctgcctgctc ttcttcgagc agcatgatca tcaccgccgc gcgttccgcc tcacccagcc
     2161 cggcaacctg ctgaagttcc tcgctcatgc cgccgtctcc ggcctggact gggcaagcat
     2221 ctggcgcagc gccaccaggg catcttcagg cttttcttcg acaattcgct gcgccaggct
     2281 gacctggcgg cccagcatgg caggatccgt tcctgtcgcc gcaggattgc cggactgcgc
     2341 cgcgctcggt tcggttccag gtgtgccatc gccgccggtc gcattctcac cgcgccgtcg
     2401 cagcacgccg atcagcggtt tgaccgccag cagcagcacc agcagcacgc cgatcagcgc
     2461 aactgcactg cgcacgacca tggcgaacca cggcgcctcg tagaacggcg gggcctcgtc
     2521 gctgatcggt tcgaacgggc ggaccagcac ctgaacctga tcgccgcgct gcggatctgc
     2581 ccccacggcc gcggtgacca gggccttgat ctgctcgatc tcttcgggtt tggccttctt
     2641 gagagccgcc tggctgatcg cgaccgccac cgaaagccgc ttgaccctgc ccggcgcggc
     2701 gttggccacc gaaacctcgc ggccaagctc gtaggtgcgc gttgcactgg attcgccgct
     2761 gcccgcgccg gctggcgcgc cgggcaccat gccgggctgc ggggcgccgg cgcgaaccgc
     2821 gctggccggc ggcggagtat tcgaaagcac tccgggcact ccgccggcct gggcgccgcc
     2881 ggtactttgc gactgggcct ggctttcgct gcggaccacg ccgtccttgt catagctttc
     2941 gcgggccgag gtaacctgat cgagatcgag ttcgatctgg atttcactgg agaacatgtt
     3001 tgcgcccagc atcggggtca gcaactggtc gatctgcgcg cgcagcttgt cttccatccg
     3061 accctgcagt tccagccggt cgttttcggc gctgcccttg gccgaaagca gccgaccgtg
     3121 ctgatcgaca acccgcaccg catccgggct catacccggg accgatccgg caaccaggtt
     3181 gaccactgcg gcaacctggc tgtcgccgag ctgccgaccg cgtgccatgc gcaccatgac
     3241 cgaggcggaa ggcggggcat tgtcgcgcac
//
LOCUS       DASUDV010000278         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1488844,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000278 DASUDV010000000
VERSION     DASUDV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488844"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFV30_06015"
     CDS             complement(<1..1166)
                     /locus_tag="VFV30_06015"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011318.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX4847680.1"
                     /translation="MSERVEKAGLKVDAALAAFVEGQVLAPLGKDPAAFWTGFAALLD
                     RFVPVNRALLAKRDDLQAQIDAWHQARAGKPIDQAEYQAFLREIGYLVPEPAPFTIGT
                     QNVDAEIATMAGPQLVVPVLNARFLLNAANARWGSLYDAYYGTDALDAPPARPGGYDT
                     DRGAAVIKAVRAFLDGAIPGWQAVFEGGSSAYLVASREGGYLFKNNGLHIEVVVDPQH
                     PVGRDDPLHIADVILESALTTICDMEDSVAAVDGEDKLAAYANWLGVIRGDLTDTFEK
                     GGRTLTRQLNGDRVWGDLTLPGRSLLFVRNVGHLMTNPAILLADGSEIPEGIMDGVIT
                     SAIGAIGLAGLGQYANSRSGSIYIVKPKQHGPEECAFTNDLFDAVEDLIGLPRHT"
     gene            1346..2557
                     /locus_tag="VFV30_06020"
     CDS             1346..2557
                     /locus_tag="VFV30_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX4847681.1"
                     /translation="MQQLSPSEAALLAAVDASPMLGQVQAWSAINTGTANLAGLAQQA
                     ALLADAFAALPGEVCLEQPGPVQAVSPAGAEVPVEYGQHLVLRVRPDAPRRFLLTGHM
                     DTVFPASDPFQSQTWLDAETLNGPGVADMKGGIAVILAALKAFETDPAAAGVGYDVMI
                     NSDEETGSLSSAPLIAALAQGKAAALTYEPSALPDGTLAGARAGSGNWSLTIRGRSAH
                     AGRNPDDGRNAVVAAAALALGLKALHRDGLSVNPAKIDGGSANNVVPDLAILRFNIRP
                     REEAAAEEFLLALKALIARIEAEHGVHVHTHGGISRPPKPLDARAEKLFGIVEECSAL
                     LGHPITRKASGGVCDGNNIAACGVPVVDTMGVRGGAIHSPDEFLIVPSLEERAKLSAL
                     VLHRLATGAPL"
     gene            2554..3585
                     /locus_tag="VFV30_06025"
     CDS             2554..3585
                     /locus_tag="VFV30_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX4847682.1"
                     /translation="MTFVIRAARADDLQHLYEMAKSTGGGFTNLPPDRKALTTKLERA
                     AQGFARTEETVGDDLFLFMLENTATGEVRGTCQIFSAVGLKWPFYSYRIGALTQHSEE
                     LGRTFRADILNLSTDMEGQSEVGGLFLHPGERSGGQGMLLARSRYLFIRKHRSRFADK
                     TFAELRGVIDESGGSPFWDGVAGRFFGMNFQEADEFNAKFGNQFIADLMPKHPVYIAM
                     LSEAARHVIGLPHPSGRAAMRMLENEGFAWENYIDIFDGGPTMTARTDQIRSVREAVD
                     ETIVAIDDRLGEHTGGEKRLCAFGQLGAFRAAYGWVEARDGGIAIDPDCARLLGVESG
                     QSITHVARW"
     gene            3589..>4010
                     /locus_tag="VFV30_06030"
     CDS             3589..>4010
                     /locus_tag="VFV30_06030"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX4847683.1"
                     /translation="MATVEINFDGIIGPSHNYAGLSLGNLASASHAGDISHPRAAALQ
                     GLAKMRHNLGLGLAQGFLLPLPRPNPRFLTAIGADGTENPRLTAAAWSASSMWTANAA
                     TVSPAADTADGRCHLTAANLVTMPHRSQEWPDTVRQLRL"
BASE COUNT          646 a         1409 c         1328 g          627 t
ORIGIN      
        1 gtgtggcgcg gcaggccgat caggtcttcc accgcatcga acaggtcgtt ggtgaaggcg
       61 cactcttccg gcccgtgctg cttgggcttg acgatgtaga tgctgcccga gcggctgttg
      121 gcgtactgcc ccagcccggc caggccgatc gcgccgatcg cgctggtgat cacgccgtcc
      181 atgatccctt ccggaatctc gctgccatcg gccagcagga tcgccgggtt ggtcatcagg
      241 tggccgacat tgcgcacgaa cagcaggctg cgccccggca gggtcagatc gccccagacc
      301 cggtcgccgt tcagctggcg ggtcagggtg cggccgccct tttcgaacgt gtcggtcaga
      361 tcgccgcgga tcacgcccag ccagttggca taggccgcca gcttgtcctc gccgtccacc
      421 gccgcgaccg aatcctccat gtcgcagatc gtggtcaggg cgctttccag gatcacgtcg
      481 gcgatatgga gcgggtcgtc cctgcccacc ggatgctgcg gatcaaccac aacttcgatg
      541 tgcaggccgt tgttcttgaa caggtatccg ccctcgcgcg acgccaccag gtatgcgctc
      601 gatccgcctt cgaacacggc ctgccagccg ggaatcgcgc catcgaggaa ggcgcggacc
      661 gccttgatca cggccgcgcc gcggtcggtg tcatagccgc ccgggcgggc cggcggagca
      721 tccagcgcgt cggtgccgta gtaggcgtca tagaggctgc cccagcgggc gttggcggcg
      781 ttgagcagga accgggcgtt gagcaccggc accaccagct gcggcccggc catggtcgcg
      841 atttcggcat cgacgttctg cgtgccgatc gtgaacgggg ccggttcggg caccagatag
      901 ccgatctcgc gcaggaaggc ctggtactcg gcctggtcga tcggcttgcc ggcgcgggcc
      961 tggtgccatg cgtcgatctg cgcctgcagg tcgtcgcgct tggccagcag cgcgcggttg
     1021 accggaacga accggtccag cagcgcagca aagccggtcc agaacgcagc cggatccttg
     1081 cccagcggtg ctagcacctg cccctcgaca aaagcggcca gcgccgcatc gaccttcaga
     1141 cccgcctttt caacccgttc gctcacatga acctcgcaca gcaatgttcg aaggcacatg
     1201 cgtgccgccg atggtgatag tcaacatttc cggggaggat gcccccgcct atgccgcagt
     1261 tgcgaagagt tgccaaccgc tttccgtgcc ggggtttggg ccgggggttg gaagcatccg
     1321 gccactcggc tagaggcggt ttcatatgca acagctttcg ccctccgaag ccgcgctgct
     1381 ggcagccgtg gacgcctcgc cgatgctggg gcaggtccag gcgtggagcg cgatcaacac
     1441 tggtaccgcc aacctggccg ggctggcgca gcaggccgcg ttgctggccg atgcctttgc
     1501 cgccctgccg ggcgaagtct gcctggaaca gccgggtccg gttcaggcgg tttcccccgc
     1561 cggggcagaa gtgccggtgg aatacggcca gcacctggtc ctgcgcgtcc ggccggatgc
     1621 gccgcggcgg ttcctgctga ccgggcacat ggacacggtc tttccggcca gcgatccctt
     1681 ccagagccag acctggctcg acgcggagac gctcaacggc cccggcgtgg ccgacatgaa
     1741 gggcgggatc gcggtgatcc tggccgcgct gaaggccttt gaaaccgatc cggccgcggc
     1801 cggcgtcggt tatgacgtga tgatcaattc ggacgaggag accggcagcc tctcctccgc
     1861 gccgctgatc gccgccctgg cccagggcaa ggccgccgcc ctcacctatg aaccctctgc
     1921 cctgcccgat ggcacgctgg caggggcgcg ggccggcagc ggcaactgga gcctgacgat
     1981 ccgcggccgt tccgcccacg ccgggcgcaa tccggatgac ggccgcaacg cggtggtcgc
     2041 cgcagcggcc ctggccctcg gcctgaaggc cctgcaccgc gacgggctga gcgtgaaccc
     2101 ggcgaagatc gacggcggca gcgccaacaa cgtggtgccg gacctggcga tcctgcgctt
     2161 caacatccgc ccccgcgaag aggctgccgc cgaggaattc ctgctggcgc tgaaggctct
     2221 gatcgcccgg atcgaggccg agcacggcgt ccacgtccac acccacggcg ggatcagccg
     2281 cccgcccaag ccgctggatg cccgggccga aaagctgttc ggcatcgtcg aggaatgcag
     2341 cgcgctgctc ggccatccga tcacccgcaa ggccagcggc ggggtctgcg atggcaacaa
     2401 cattgccgcc tgcggtgtgc ccgtggtcga taccatgggc gtgcgcggcg gcgcgatcca
     2461 ttcaccggac gaattcctga tcgtccccag tctggaggaa cgcgcgaaac tctctgcgct
     2521 ggtccttcac cgtctagcta ccggagcccc cctgtgacct tcgtgatccg cgccgcgcgc
     2581 gccgacgacc ttcagcacct ctacgagatg gccaaaagca ccggcggcgg gttcaccaac
     2641 ctgcccccgg accgcaaggc gctgaccacc aagctggagc gcgcggcgca gggctttgcc
     2701 cgcaccgaag agacggtggg cgacgacctg ttcctgttca tgctggaaaa caccgcgacc
     2761 ggcgaagtgc gcggcacctg ccagatcttc tctgccgtcg ggctgaagtg gccgttctat
     2821 tcctaccgga tcggcgcgct gacccagcac agcgaggaac tgggccggac cttccgcgcc
     2881 gacatcctca acctctccac cgacatggag ggacagtccg aagtgggcgg gctgttcctg
     2941 catccgggcg aacgctcggg cgggcaaggc atgctgctgg cccgcagccg ctatctgttc
     3001 atccgcaagc accgcagccg ctttgccgac aagacctttg ccgaactgcg cggggtgatc
     3061 gacgaaagcg gcggatcgcc gttctgggac ggggtggccg ggcgcttctt cgggatgaat
     3121 ttccaggaag ccgacgaatt caacgccaag ttcggcaacc agttcatcgc cgatctgatg
     3181 cccaagcatc cggtctacat cgccatgctg agcgaggccg cgcgccacgt gatcggcctg
     3241 ccccacccca gcggccgcgc ggcgatgcgg atgctggaga acgagggctt tgcctgggaa
     3301 aactacatcg acatcttcga tggcggccca accatgaccg cccggaccga ccagatccgt
     3361 tcggtgcgcg aggcggtgga cgagacgatc gtcgctatcg acgaccgcct gggcgagcac
     3421 acgggcggcg aaaagcggct ctgcgccttc ggccagctgg gcgcgttccg cgcggcctat
     3481 ggctgggtcg aggcccgcga cggcggcatc gcgatcgacc cggactgcgc ccgcttgctg
     3541 ggtgtcgaaa gcggccagtc gatcacccac gtggcacggt ggtgaaccat ggcaacggtt
     3601 gaaatcaact tcgacgggat catcggtccc agccacaatt acgccgggct gagcctgggc
     3661 aatctggcct cggccagcca cgccggcgat atctcgcacc cgcgcgccgc ggcgctgcag
     3721 gggctggcca agatgcgcca caacctgggc ctgggcctgg cccagggctt ccttctgccc
     3781 ctgccccggc ccaacccccg cttccttacc gcaatcggcg cggacgggac cgagaatccg
     3841 cggctgaccg cggcggcctg gtcggccagc tcgatgtgga cggccaatgc cgccaccgtt
     3901 tcgcccgccg ccgatacggc cgacgggcgc tgccacctga ccgcggccaa cctggtcacc
     3961 atgccgcacc gcagccagga atggcccgac acggtgcgcc agttgcgcct
//
LOCUS       DASUDV010000279         6093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1290411,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000279 DASUDV010000000
VERSION     DASUDV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290411"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..320)
                     /locus_tag="VFV30_06035"
     CDS             complement(147..320)
                     /locus_tag="VFV30_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX4847684.1"
                     /translation="MKFIKKLFRNEDGATAIEYGLIAALISVAAIGAMSSLGGELSST
                     FNHTSSTMANARA"
     gene            complement(422..604)
                     /locus_tag="VFV30_06040"
     CDS             complement(422..604)
                     /locus_tag="VFV30_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX4847685.1"
                     /translation="MIKTITRMIADTRAATAVEMGLVVALLVVAVMGAMQAFAGEAIN
                     LWDDIAAKSRDANSRP"
     gene            685..1101
                     /locus_tag="VFV30_06045"
     CDS             685..1101
                     /locus_tag="VFV30_06045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX4847686.1"
                     /translation="MKKIPTVIPVVAVALIDPQGRVLMHQRRRASEHGGLWEFPGGKV
                     ETGESLAESLCREISEELGIALDPAALEPLSFAASDDLPYVILLYTCRRWSGEPACLE
                     GEAIGWFAPDAIAALPMPPLDVPLARALTEHLAKAI"
     gene            1147..1223
                     /locus_tag="VFV30_06050"
     tRNA            1147..1223
                     /locus_tag="VFV30_06050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1181..1183,aa:Arg,seq:acg)
     gene            complement(1401..2117)
                     /locus_tag="VFV30_06055"
     CDS             complement(1401..2117)
                     /locus_tag="VFV30_06055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4847687.1"
                     /translation="MDRFEQLRRLARLVPGGMAAGRAARRWLDPDLREIIRLKREESA
                     ALLQPFPDTFEDRYPALFDALALRLADHPAPRILSFGCSSGAEIRALRRRLPNARFTG
                     IDPNRRALARARAADPHGDYRAGTAPAEGDSFDAILAMAVLRHGALEAERPDRCDAVL
                     PFERVAETVAGLERSLAPGGWLAVGNAHFRVSDCPAAAALWPDPQDFGQLSRPSLLYG
                     ADGRRIESPAPVPVLFHKPD"
     gene            2205..3698
                     /locus_tag="VFV30_06060"
     CDS             2205..3698
                     /locus_tag="VFV30_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX4847688.1"
                     /translation="MALPETDPAPSPSVWRFARASRAHVVIDAAAYFAHMQQAMLAAR
                     QRIHMIGWDFDTRVRLGPGRKIYNLPDRDVQPARLGPFVLWLVRRTKGLQVRVLKWNF
                     GALKFIFRGSMILDLIRWFLSPAIDFKFDAAHPLGCSHHQKIIVIDDRFAVCGGIDMT
                     SDRWDTPEHLEEDPRRRRPYGTRLYGPWHDLSMLVEGDAAAALAELGRERWTQAGGAP
                     LLPCAPQPETPWPANLKAEFRDVEVGIARTRSAWAGLDEVREIEALFVEHIRRAKRFI
                     YAESQYFASRAVAEAIAERVAEPDPPEIVLINPESAEGWLEQAAMDGARVRLCHAIAE
                     RDHRQRFRIFIPYNSAGTPIYVHSKTMIVDDEIVRIGSANMNNRSMGLDSECDLFIDA
                     ARPGNDHARAPIERLRITLLAEHLGMAPEKVAAELAALGSMIALIETAPKDRKRLEPF
                     GLRPLTDAEKAVADNALLDPERPEELFEPFSRKRGLFRRGGFLLRPR"
     gene            3710..4318
                     /gene="folE"
                     /locus_tag="VFV30_06065"
     CDS             3710..4318
                     /gene="folE"
                     /locus_tag="VFV30_06065"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX4847689.1"
                     /translation="MSCAHDGPDEGPMDKIPVPDDVQDAIRTLIRWSGDDPAREGLLD
                     TPARVARAWKEYCGGYEADPAHHLARQFEEVGGYDEVVLLKDIPFQSHCEHHMAPITG
                     KAAIAYMPRDRVVGISKLARVLHGYARRLQIQERLTAEVAQCIWDNLNPHGVAVVIEA
                     QHGCMTGRGVKTPGVGMVTSRLMGCFLDDHRSRKEVLSLMGY"
     gene            4324..4749
                     /locus_tag="VFV30_06070"
     CDS             4324..4749
                     /locus_tag="VFV30_06070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4847690.1"
                     /translation="MAAEPVMTVEALEAFFRAAFPDVPEAAHARIDAIEPGRVRLTLP
                     EAALGLRPGGIVSGPTQMALADRAAYAVILAHIGPVAMAVTSNLNISFLRAVEAKPVC
                     ADAVLLKLGRRLATVDVRLWQDSEATVLAQASVTYAIPG"
     gene            complement(4944..5300)
                     /locus_tag="VFV30_06075"
     CDS             complement(4944..5300)
                     /locus_tag="VFV30_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEX4847691.1"
                     /translation="MDWGQLAIKALVSGVLIAAASELARRSPGWGGLIASLPLVSTLA
                     MIWLWRDTGDGGRVAAMAVSSSLYVLASLPAFAVIALLLRKGIAFPAALIAFALTGWL
                     GYWLMQVLGRRWGWPV"
     gene            complement(5305..5784)
                     /locus_tag="VFV30_06080"
     CDS             complement(5305..5784)
                     /locus_tag="VFV30_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4847692.1"
                     /translation="MTIAVGDKLPDVKLVKATEAGPEAVQSAEFFAGKRVALFSVPGA
                     FTPTCSAKHLPGFVEKAADLKAKGIDEIACTAVNDPFVMGAWGKAGGSEDVTMLADGN
                     GDFAEALGLVMDGSAFGLGKRGQRFSMVVNDGTVEQLHVEGPGEFKVSSAEYMLEQL"
BASE COUNT         1029 a         1966 c         2105 g          993 t
ORIGIN      
        1 gtgctgaacg ggctgcaggc caatgcgcgg attaccgcgg ggcagcggat caagctggtt
       61 acctactgat tttcgcgggt ttcgggcaaa aagaaagggc ggcaggtttc cctgccgccc
      121 tcgcttctcg aatggcttcg cggcgcttag gcgcgggcgt tcgccatggt gctcgaggtg
      181 tggttgaagg tgctcgagag ctcgccaccc aggctgctca tggcgccgat ggcggcaacc
      241 gagatcagag cggcgatcag gccgtattcg atggcagtcg caccgtcttc gttgcggaac
      301 agcttcttga tgaacttcat ggtcagtctc ctagtaggca gtcaatttac ccggctcttt
      361 ccctgtcacc cggattgagc aaacaccgga ctaaggggaa gttcttgaaa aaggtttaac
      421 gtcagggccg cgaattcgcg tcgcggctct tggcggcgat atcgtcccac aggttgatgg
      481 cttcgccggc gaaggcctgc atcgccccca tcacggcgac gacgagcagc gccaccacga
      541 gacccatttc gacagcggtc gcggcgcgcg tgtcggcgat cattctggta atggtcttga
      601 tcactggctt catccccgct ttgggtgctt tgcaggccag ttaaggagcg catcgctaac
      661 aattggttta gacgggggcc taacgtgaaa aaaatcccga cagtgatccc cgtggtggcg
      721 gtggccttga tcgacccgca agggcgggtg ctgatgcacc agcgccgccg cgccagcgaa
      781 cacggcgggc tgtgggagtt tcccggcggc aaggtggaaa cgggagaatc gctggccgaa
      841 tcgctctgcc gcgagatttc cgaagagctg gggattgctc tcgatcccgc cgcgctggag
      901 ccactgagtt tcgccgcaag cgacgatctg ccctatgtca tcctgcttta cacctgccgc
      961 cgctggtcgg gcgagccggc ctgcctggaa ggcgaggcga tcggctggtt cgcgccggat
     1021 gcgattgcgg cgctgccgat gcccccgctc gacgtgccgc tggcaagggc gctgacggaa
     1081 catctcgcca aggcaattta gcggcttgcc aagcgcaaac gacctgccta tgtgcgccac
     1141 tccaacgcgc ccgtagctca gctggataga gcatcagact acgaatctga gggtcggacg
     1201 ttcgaatcgt tccgggcgcg ccattaccaa aagaggctca ccgcaaggtg ggcctttttt
     1261 ggtaacgggg ggtcacggac cggggcggac cttcggagtc ggagcgcagc ggagacgacc
     1321 gagcccgcca gggcgagggc aatcgttccg ggcgcgtcct taccaaaaga ggctcaccgc
     1381 aaggtgggcc ttttttgttt tcagtccggt ttgtggaaca ggaccgggac cggggcgggg
     1441 ctctcgatcc ggcgcccgtc tgcaccgtag agcaggctgg gccggctgag ctggccgaag
     1501 tcctgcggat cgggccagag cgccgccgcg gccgggcagt cgctgacccg gaaatgggcg
     1561 ttgcccaccg ccagccatcc gccgggggcc aggctccgct ccagccccgc cacggtttcg
     1621 gccacccgtt caaacggcag caccgcatcg cagcgatccg ggcgctccgc ttccagcgcg
     1681 ccgtggcgca gcaccgccat ggccaggatc gcgtcaaagc tgtcgccctc cgccggggcc
     1741 gtcccggcgc ggtagtcgcc gtgcggatcg gccgcccggg cccgggccag cgcgcggcgg
     1801 ttgggatcga taccggtaaa gcgggcgtta ggcaggcggc ggcgcagggc ccggatctcg
     1861 gccccgctgg aacagccgaa cgacaggatc cgcggggcgg ggtgatcggc cagccgcagg
     1921 gccagggcat cgaacagggc cggatagcgg tcctcgaacg tgtcggggaa gggctgcagc
     1981 agggcggcgc tctcttcgcg tttcagccgg atgatctcgc gcaggtccgg atccagccag
     2041 cgccgcgccg cgcgccccgc ggccatgccg cccggcacaa ggcgcgcaag ccgccgcaat
     2101 tgttcgaacc ggtccatcat cggccgtcct ttcgcacagg ccgcgccgcc gggaaagcgg
     2161 cgagagggcg tgacggcgca gcgcggcggg gctatgggga gggcatggcc ttgccggaaa
     2221 ccgaccccgc accgtctccg tcggtctggc gctttgcccg ggccagccgc gcccacgtgg
     2281 tcatcgatgc cgcggcctat ttcgcgcaca tgcagcaggc gatgctggcg gcccggcagc
     2341 ggatccacat gatcgggtgg gatttcgata cccgcgtgcg gttggggccg gggcgcaaga
     2401 tctacaatct gcccgaccgc gatgtgcagc cggcccgcct ggggcccttc gtgctgtggc
     2461 tggtgcggcg caccaagggc ctgcaggtgc gggtgctgaa atggaacttc ggcgcgctga
     2521 agttcatttt ccggggctcg atgatcctgg acctgatccg ctggttcctc agcccggcga
     2581 tcgatttcaa gttcgacgcc gcccacccgc tgggctgcag ccatcaccag aagatcatcg
     2641 tgatcgacga tcgctttgcg gtctgcggcg ggatcgacat gaccagcgac cgctgggaca
     2701 cgcccgagca cctggaagag gacccgcggc ggcggcggcc ctatggcacg cggctctatg
     2761 gtccgtggca cgatctttcg atgctggtgg agggcgatgc ggccgcggcc ctggccgaac
     2821 tggggcggga acgctggacc caggccggcg gggcgccgct gctgccctgc gcgccccagc
     2881 cggaaacgcc ctggccggcc aacctcaagg ccgaattccg cgatgtcgaa gtgggcatcg
     2941 cccggacccg ctcggcctgg gcgggtctgg acgaggtgcg cgagatcgag gccctgttcg
     3001 tggagcatat ccgccgggcc aagcggttca tctatgccga gagccagtac ttcgccagcc
     3061 gcgccgtggc cgaggcgatt gccgagcggg tggccgagcc cgatcccccg gaaatcgtgc
     3121 tgatcaatcc ggaaagcgcc gaagggtggc tggaacaggc ggcgatggac ggggcccggg
     3181 tgcggctgtg ccacgcgatt gcggagcgcg accaccgcca gcggttccgg atcttcattc
     3241 cctacaattc ggccggcacg ccgatctatg tccattccaa gacgatgatc gtcgatgacg
     3301 agatcgtgcg gatcggttcg gccaacatga acaaccggtc gatggggctg gacagcgaat
     3361 gcgacctgtt catcgatgcc gcccgccccg gcaacgatca cgcccgcgcc ccgatcgagc
     3421 ggctgcggat cacgctgctg gccgaacacc tggggatggc gccggaaaag gtggcggccg
     3481 aactggccgc gctgggatcg atgatcgcgc tgatcgagac tgcccctaaa gacaggaagc
     3541 ggctggagcc ttttggcttg cgtccgctga ccgatgcgga gaaagcggtg gccgacaacg
     3601 cgttgctcga tccggaacgg cccgaggaac tgttcgaacc gttcagccgc aagcgcggcc
     3661 tgtttcgccg gggcggcttt ctgctgcgcc cgcgctgagg gagatgagga tgtcttgcgc
     3721 gcacgatggc ccggacgaag ggccgatgga caagattccg gtgccggatg acgtgcagga
     3781 tgcaatccgc acgctgatcc gctggtcggg cgacgatccg gcccgcgaag gcctgctcga
     3841 tacgccggcc cgcgtcgcgc gcgcctggaa ggaatactgc ggtggctacg aagccgatcc
     3901 ggcgcaccac ctggcccgcc agttcgagga agtgggcggc tatgacgaag tcgtgctgct
     3961 gaaggacatc cccttccagt cgcactgcga acaccacatg gccccgatca ccggcaaggc
     4021 ggcgattgcc tatatgccgc gcgaccgggt ggtggggatt tccaagctgg cgcgggtgct
     4081 gcacggctat gcccggcgcc tgcagatcca ggaacgcctg accgccgaag tggcgcagtg
     4141 catctgggac aacctcaatc cgcacggcgt tgccgtggtg atcgaggcgc agcacggctg
     4201 catgaccggc cgcggggtca agactccggg cgtcggcatg gtcaccagcc gcctgatggg
     4261 ctgcttcctg gacgaccacc gcagccgcaa ggaagtgctc agcctgatgg gctattgatc
     4321 gccgtggccg cggagccggt catgacggtc gaggcgctgg aagccttctt ccgcgccgct
     4381 tttcccgatg tgcccgaggc ggcccacgcc cggatcgatg cgatcgagcc gggccgggtc
     4441 cgcctgaccc tgcccgaagc cgcgctgggc ctgcgcccgg gtggtatcgt gtccggcccg
     4501 acccagatgg ccctggccga ccgggccgcc tatgcggtga tcctggcgca tatcggcccg
     4561 gtcgccatgg cggtgaccag caacctcaac atcagcttcc tgcgcgcggt ggaggcaaag
     4621 ccggtctgcg ctgacgcggt tctgctcaag ctggggcggc ggctcgccac ggtcgacgtg
     4681 cggctgtggc aggacagcga ggccacggtt ctggcccagg ccagcgtcac ttacgccatt
     4741 cccggctagt ccggaaaaca gaaggcccgg aagaccgttg cttgtcttcc gggccagtag
     4801 agggcttggg gggagatcca tccctctggg tcgcagcacc ttaatcgctc gattaagggc
     4861 gattcgcttt gacgcgcgtc aaaaatcctc ccgcagcgcg ggacgggcga aaaggcgtca
     4921 ggaagggcag ggggaggagg cggctagacc ggccagcccc agcggcggcc gaggacctgc
     4981 atcagccaat agcccagcca gccggtgagc gcgaaggcga tcagggcggc gggaaaggcg
     5041 atccccttgc gcagcaaaag ggcgatcacc gcaaaagcgg gaagggaggc cagcacatag
     5101 aggctggagg aaacggccat ggcggcaacc cgcccgccat cgccggtatc gcgccacagc
     5161 cagatcatcg ccagggtgga gacgagcggc aggctggcga tcagcccgcc ccagccgggg
     5221 ctgcggcggg ccagttcgga ggcagcggca atcaggacgc ccgaaacgag cgccttgatt
     5281 gccagctgcc cccaatccat cggcttagag ctgctcgagc atgtactcgg cgctcgagac
     5341 cttgaactcg ccggggcctt cgacgtggag ctgctcgacg gtgccgtcgt tcaccaccat
     5401 cgagaagcgc tggccgcgct tgcccaggcc aaaggcgctg ccgtccatca ccaggcccag
     5461 ggcttcggcg aaatcgccgt tgccgtcggc cagcatggtc acgtcttcgc tgccgccggc
     5521 cttgccccag gcgcccatca cgaagggatc gttgaccgcg gtgcaggcga tctcgtcgat
     5581 ccccttggcc ttgaggtcag cggccttttc gacaaagccc ggcaggtgct tggccgagca
     5641 ggtcggggtg aaagcccccg ggaccgagaa cagcgccacg cgcttgccgg cgaagaattc
     5701 ggcgctctgc accgcttcgg ggccggcttc ggtggccttg accagtttca catcgggcag
     5761 cttgtcgcca acagcgatcg tcatcatgcg tctccttgca gggggttctt gcgattctct
     5821 cgcaggatat agggtgccgt ggctccgcga caacccgcag gatcgtggta aatttcgctt
     5881 tactgcgccg ctttaagaac gggtaaacag cctgagcgat gctgtgtctt gcggggatca
     5941 acccgtcggg agactgcaca tgaaaatcgt aaccggggtc gcggccggtc tggccgccgc
     6001 atcaatgctt ttgtccgttc cggtcagcgc caattccggc atgagccagg ccgagaaact
     6061 gcgccggctc gacatcatgc tgatggtcac cgg
//
LOCUS       DASUDV010000280         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_141498,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000280 DASUDV010000000
VERSION     DASUDV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141498"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1827
                     /locus_tag="VFV30_06085"
     CDS             124..1827
                     /locus_tag="VFV30_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4847693.1"
                     /translation="MSVNSTAAPSAADPWRHHYGHPGAWDLDLPPLSLPAMFAAAAAR
                     HGPRPLVDFLGRTYSYAALHAEAQAFAAGLQRLGVKKGDRVGLFLPNVPIYVSAYYGA
                     ALAGATLVNFSPLYTAQELAAQVEDSGARLLVTIDSAALLPTALEVQRTSSLETLVVG
                     SLGAMLPWTKRVLLKVFGRKAVVPIPAGPSVLRWAEVLGPGAPQPVPLDPAADLALLQ
                     YTGGTTGTPKGAMLTHQNLTANTRQVNALDPQTSERDVIVAVLPLFHVFANIAVLNRT
                     VLNGGCIAMLPRFEAGQALKTLDRVQATSFPGVPTMYQALLDHPSVGKTDFSSLRVCI
                     SGGAPLPLPLKQRFEAVSGARIVEGYGLTECAGVASCNPYEGEERPGTIGQPIPATRV
                     RLLDREDPTRDAAPGEPGELAIQGPQVMQGYWNRPEAAAAAFAERPDGRWLRTGDVAT
                     IDADGYISIVDRIKDMIAVGGFKVFPSQVEAVLEEHPAVKEVLVIGVPEERLGEVPRA
                     YVTLRDGADTTGEALRDWLNARIGKHERVDAVVVRESLPKTMIGKLDRKALRAELAAA
                     G"
     gene            complement(1843..2196)
                     /locus_tag="VFV30_06090"
     CDS             complement(1843..2196)
                     /locus_tag="VFV30_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4847694.1"
                     /translation="MANSTFSVFGADIRIKGDVHASADLHIDGKVEGDVACTALVQGE
                     SGEIVGAVKAESARLSGTVRGTITVRDLVVLKTARIHGDVHYDSLTIEQGAQVDGRFA
                     PRAVGEDSAVVSFPG"
     gene            complement(2201..>2716)
                     /locus_tag="VFV30_06095"
     CDS             complement(2201..>2716)
                     /locus_tag="VFV30_06095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4847695.1"
                     /translation="SIDPRFQRFGLSLARMAALERSVAALPQALPASLEYISSGFGYR
                     SDPFTGGADFHPGLDFKGPMGAPIYAAAKGTVSFVGWKSGYGNVVEIDHGNGLVTRYA
                     HMSGFRTVAGKPVMPGELIGLIGSTGRSTGPHLHFEVRVHDQPVNPRPFLEAVPNVFQ
                     EAGGNAAQRGR"
BASE COUNT          426 a          935 c          945 g          410 t
ORIGIN      
        1 agccgcgcag gaggccgaag cggccgcctg gcttgaggca aagcgtgccg ccgaagcggc
       61 gggcgaggaa taaaatcctg acagggcata ggctttcgcc cggcgaacgc ctatgctctt
      121 tacatgagtg tcaatagcac cgcggcccca tccgccgccg atccctggcg ccaccactac
      181 ggccaccccg gcgcgtggga tctggacctg ccgccgctca gcctgccggc catgttcgct
      241 gccgccgcgg cccgccacgg gccccgcccg ctggtcgact tcctcggccg gacctacagc
      301 tatgccgcgc ttcacgccga ggcccaggcc tttgccgccg ggctgcagcg gctgggggtg
      361 aagaagggcg accgggtcgg cctgtttctg cccaatgttc cgatctatgt ttcggcctat
      421 tacggcgcgg ccctggccgg agcgacgctg gtcaatttca gcccgctcta caccgcgcag
      481 gaactggccg cgcaggtcga ggattcgggc gcgcgcctgc tggtgacgat cgattcggcc
      541 gcgctgctcc ccaccgcgct ggaagtgcag cggacctcct cgctcgaaac gctggtcgtc
      601 ggctcgctgg gggcgatgct gccctggacc aagcgggtct tgctgaaggt cttcggccgc
      661 aaggcagtgg tgccgatccc ggccgggccc agcgtgctgc gctgggccga agtgctcggc
      721 cccggcgcgc cgcagcccgt gccgctcgat cccgccgcgg acctcgcatt gctgcaatac
      781 accggcggaa ccaccggcac gcccaagggg gcgatgctca ctcaccagaa cctcaccgcc
      841 aacacccggc aggtcaacgc gctcgacccg cagaccagcg agcgcgacgt gattgtcgcg
      901 gttctgccgc tgttccacgt cttcgccaac atcgcggtgc tcaaccgcac ggtgctgaac
      961 ggcgggtgca tcgcgatgct gccgcggttc gaggccggcc aggcgctgaa gacgctggac
     1021 cgggtccagg ccacttcgtt cccgggcgtg ccgaccatgt accaggcgct gctcgatcat
     1081 ccttcggtcg gcaagaccga cttttcctcg cttagggtct gcatttcggg cggggcgccg
     1141 ctgccgctcc cgctcaagca gcgtttcgag gcggtcagcg gggcgcggat cgtcgaaggg
     1201 tacggactca ccgaatgcgc cggggtggcc tcgtgcaatc cctatgaggg cgaggagcgg
     1261 cccggtacga tcggccagcc gatccccgcc acccgagtcc gcctgctcga ccgcgaagac
     1321 cccacccgcg atgccgcgcc gggcgaaccg ggcgaactgg cgatccaggg accgcaggtg
     1381 atgcagggct actggaaccg ccccgaagcg gccgccgccg cctttgccga gcggcccgac
     1441 gggcgctggc tgcggaccgg ggatgtcgcg acgatcgacg ccgatggcta catttcgatc
     1501 gtcgaccgga tcaaggacat gatcgccgtg ggcggcttca aggtcttccc cagccaggtc
     1561 gaggccgtgc tggaagagca tccggcggtg aaggaagtgc tggtgatcgg cgtgcccgag
     1621 gaacgcctgg gcgaagtgcc gcgggcctat gtcacgctgc gcgacggtgc cgacaccact
     1681 ggcgaggcgc tgcgcgactg gctcaacgcg cgcatcggca agcacgagcg ggtcgatgcc
     1741 gtggtggtgc gcgaaagcct gcccaagacg atgatcggca agctcgaccg caaggcgctg
     1801 cgcgcggaac tggctgccgc cgggtgacgg cggcggcggg gatcagcccg ggaagctgac
     1861 gaccgcgctg tcttcgccga ccgcgcgcgg ggcgaagcgg ccgtcgacct gtgcgccctg
     1921 ctcgatcgtc agcgaatcgt aatggacatc gccgtggatc cgggcggtct tcagcaccac
     1981 caggtcgcgc acggtgatcg tgccgcggac cgtgcccgac agccgggcgc tctcggcctt
     2041 gaccgcgccg acaatctcgc cgctttcgcc ctggaccagc gcggtgcagg cgacatcgcc
     2101 ttccaccttg ccgtcgatgt gcagatcggc cgaggcgtgg acatcgccct tgatgcggat
     2161 gtccgcgccg aacaccgaaa acgtcgaatt ggccatggtt ctacctgccg cgctgtgcgg
     2221 cgttgccgcc ggcttcttgg aaaacattgg gtacagcctc aaggaagggg cggggattga
     2281 ccggctggtc gtggacccgc acttcgaaat gaaggtgcgg cccggtcgag cggccggtgc
     2341 tgccgatcag gccgatcagc tcgcccggca tgaccggctt gccggccacg gtgcgaaagc
     2401 ccgacatgtg ggcatagcgg gtgaccaggc cgttgccgtg atcgatctcg accacgttgc
     2461 catagcccga tttccagccg acgaagctga ccgtgccctt ggcagcggca tagatcggcg
     2521 cgcccatcgg gcccttgaaa tcgaggccgg ggtggaaatc ggccccgccg gtgaagggat
     2581 cggagcggta gccgaagccc gaggaaatgt attcgaggct ggccggcagc gcctggggca
     2641 gggccgcgac cgagcgctcc agcgcggcca tccgcgccag gctcaggccg aagcgctgaa
     2701 agcgcgggtc gatcga
//
LOCUS       DASUDV010000281         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1732273,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000281 DASUDV010000000
VERSION     DASUDV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732273"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFV30_06100"
     CDS             <1..1464
                     /locus_tag="VFV30_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4847696.1"
                     /translation="QHDNRIYRPPFYSRLYSAHSQLPDFLINTHTVADETDLKNYIAR
                     LRAMPAVLDLAIARSKVSDAAGIRAPRFQIETVIAGSEKLTTGAPFGPGPDAALWADV
                     QAKTGALVKAGKLTQAEGEAHLQQARAAILDLKPAYDRVIAWARGNLATAPSGRVGAV
                     TLPGGLAYYANELKLNTTTDLTADQIHAIGLSEVARIEAEQDALARQAGFADRHAFYA
                     ERARLYPPVPYTDALRKEYLDTANAFVAHTRTLLGPYFGTLPAYGIEVVREPAFSEVP
                     GGAAHASAPSPDGKRPARTYVHLAGMRPDPAAMYTLMCHEAVPGHNMQGDIQVRQQGG
                     PKFRAVTGYVAFGEGWALYAEALCKEMGAFPDVAADFMRLDAELFRAARLVVDTGLHA
                     KGWTEEEAVTYMHKTGRQPIERSRSEVRRYITLPGQATGYKIGMLKIMELRAKAEREL
                     GPKFDIKGFHDLLIASGSQPLSILERRVNDWIAKRKG"
     gene            1484..2293
                     /locus_tag="VFV30_06105"
     CDS             1484..2293
                     /locus_tag="VFV30_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4847697.1"
                     /translation="MNQFEYIDGQLRPAHPALFEAFAAESEAAVQSAQPTLDLAYGPH
                     PRQRLDLFRASGPRRGAIAYFHAGYWQSRDKSGFRFLAPPLAADGFDVAIVNYPLSPE
                     AGVGDIVCTAAAALPALAALTDHAPQILVGHSAGAQIAAELAMAPPPGAATVAGIVSI
                     SGVFDLEPLIGTTVNAKLGLDPQTARAASPVHRVTPDAPPTLFAVGALETRSFQDQTT
                     AMAQAWAAAGNTARELTVAEADHFSILRELSREGGVLRAAIGDLLEAVPAG"
     gene            complement(2303..>2641)
                     /locus_tag="VFV30_06110"
     CDS             complement(2303..>2641)
                     /locus_tag="VFV30_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4847698.1"
                     /translation="DGALTEVLGLVEKPKREEAPSNLIISGRYILQPEVMRVLESQGK
                     GAGGEIQLTDAMARMIGQQPFHAVTFAGKRYDCGSKTGFVEATLAIALEREDMADDVR
                     AMAKRLLAEG"
BASE COUNT          413 a          970 c          867 g          391 t
ORIGIN      
        1 cagcatgaca atcgcatcta tcgcccgccc ttctattcgc ggctctattc ggcgcacagc
       61 cagctgcccg atttcctgat caacacccac accgtggccg atgagaccga cctgaagaac
      121 tacatcgccc ggctgcgcgc catgcccgcg gtgctcgatc tggcgatcgc gcgcagcaag
      181 gtgagcgacg ccgccgggat ccgcgcgccg cggttccaga tcgaaacggt catcgccggc
      241 agcgagaagc tgaccaccgg ggcgccgttc ggccccggcc cggacgccgc gctgtgggcc
      301 gacgtgcagg ccaagaccgg cgcgctggtc aaggccggca agctgaccca ggccgagggc
      361 gaagcgcacc ttcagcaggc gcgcgccgcg atcctggatc tcaaacccgc ctatgaccgg
      421 gtgatcgcct gggccagggg caacctggcg accgcgccca gcggccgggt cggcgcggtc
      481 accctgccgg ggggcctcgc ctattacgcc aacgagctga agctcaacac cacgaccgac
      541 ctgaccgccg accagatcca cgccatcggc ctgtcggaag tggcgcggat cgaggcggag
      601 caggatgcgc tggcccgcca ggccggcttt gccgaccgcc acgccttcta cgccgagcgg
      661 gcgcggctct atccgccggt tccctatacc gacgcgctgc gcaaggaata cctcgacacc
      721 gccaacgcct ttgtcgccca cacccgcacg ctgctggggc cctacttcgg cacgctgccg
      781 gcctatggga tcgaagtggt gcgcgaaccg gccttttccg aagtgccggg cggggctgcc
      841 catgcctcag cccccagccc cgatggcaag cgcccggcgc ggacctatgt ccacctggcg
      901 ggcatgcggc ccgatccggc cgcgatgtac accctgatgt gccacgaggc cgtgcccggc
      961 cacaacatgc aaggcgatat ccaggtgcgc cagcagggcg gtccgaagtt ccgcgcggtg
     1021 accggctatg tcgctttcgg cgaaggctgg gcgctctatg ccgaggcgct gtgcaaggaa
     1081 atgggcgcct tcccggatgt cgcggccgat ttcatgcggc tggatgccga actgttccgc
     1141 gccgcgcggc tggtggtcga taccggcctc cacgccaagg gctggaccga ggaagaggcg
     1201 gtcacctaca tgcacaagac cggccgccag ccgatcgagc gctcgcgctc cgaagtgcgc
     1261 cgctacatca cgctgcccgg gcaggccacc ggctacaaga tcggcatgct caagatcatg
     1321 gagctgcgcg ccaaggccga gcgcgagctg gggccgaagt tcgatatcaa gggcttccac
     1381 gatctgctga tcgcgtcggg ttcgcagccg ctttccatcc tggagcggcg ggtgaacgac
     1441 tggatcgcga agcgcaaagg ctgagacccg gggccaaagc gcgatgaacc agttcgaata
     1501 catcgacggg cagctgcgcc cggcgcaccc ggcgctgttc gaagcctttg cggcggagag
     1561 cgaagccgcg gtgcagtcgg cgcagccgac gctcgacctt gcctatggcc cgcacccgcg
     1621 ccagcggctc gacctgttcc gcgcgagtgg tccgcggcgc ggcgcgatcg cctatttcca
     1681 cgccgggtac tggcaatcgc gcgacaagag cgggttccgc ttcctcgccc cgccgctcgc
     1741 cgccgacggg tttgacgtgg ccatcgtcaa ctacccgctc agccccgagg cgggcgtcgg
     1801 cgacatcgtc tgcacggcgg cggcggcact gcccgccctt gccgcgctga ccgaccatgc
     1861 cccgcagatc ctggtcggcc attcggcggg cgcgcagatc gcggccgaac tggcgatggc
     1921 accgccgccc ggcgccgcca cggttgccgg gatcgtctcg atcagcgggg tgttcgatct
     1981 ggagccgctc atcggcacca cagtaaacgc caagctgggc cttgatccgc agaccgcccg
     2041 tgcggcatcc ccggtccacc gcgtcacccc cgacgcaccg cccaccctgt tcgcggtcgg
     2101 cgcgctcgaa acgcgctcgt ttcaggacca gaccaccgcg atggcccagg cctgggccgc
     2161 ggcgggcaac accgcgcgtg aactcaccgt ggcggaggcc gatcacttct cgatcctgcg
     2221 cgaactcagc cgcgaaggcg gcgtgctgcg cgccgcgatc ggcgatctgc tggaagcggt
     2281 gccggccggc tagggtcgcg cctcagcctt cggcgagcag ccgcttcgcc atggcccgca
     2341 cgtcgtccgc catgtcctcg cgctccagcg cgatggccag ggtcgcctcg acgaagccgg
     2401 tcttgctgcc gcagtcatag cgcttgccgg caaaggtaac ggcgtggaac ggctgctggc
     2461 cgatcatccg cgccatcgcg tcggtcagct ggatttcccc gcccgcgccc ttgccctggc
     2521 tttccagcac ccgcatcact tcgggctgca ggatatagcg gcccgagatg atcaagttcg
     2581 acggggcctc ttcgcgcttg ggcttttcga ccaggcccag cacttcggtc agcgcgccgt
     2641 c
//
LOCUS       DASUDV010000282         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_561264,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000282 DASUDV010000000
VERSION     DASUDV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561264"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VFV30_06115"
     CDS             <1..1299
                     /locus_tag="VFV30_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX4847699.1"
                     /translation="QFKRLGLVIVDEEQRFGVNHKEALKQLRNNVHVLTLTATPIPRT
                     LQMAMSGLRELSTIQTAPVDRLAVRTYVMEWDDMVMREALLREHHRGGQSFIVVPRIA
                     DMAEVEDWLHKTVPEVRYISAHGQMAAGEVEERMSAFYEKKYEVLLSTTIVESGLDIP
                     SANTIIIHRADRFGLAQLYQLRGRVGRAKLRAYAYLTTPADRQLSEVAEKRLKVLGDL
                     DSLGAGFQLASHDLDIRGAGNLLGDEQSGHIREVGFELYQSMLEDAILAAKAGDLGVV
                     KERHALSPQITVDAPIMIPEDYVPDLAVRMALYRRLNDAEDTGEVEALSAEMIDRFGP
                     LPQPTANLIKLIQIKRQAIEANIAKIDVGPKGTLVSFHNDEFPDPAGLLNYAQRLEGT
                     IKLRPDSKIVVVRAWGSAEARLNGLFQLTKGLSAIARKAR"
     gene            complement(1325..2611)
                     /locus_tag="VFV30_06120"
     CDS             complement(1325..2611)
                     /locus_tag="VFV30_06120"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEX4847700.1"
                     /translation="MDALQSEPLPLRSDALTGLPGRDAARARLAEWLDRGEPVHALLI
                     GLRRFDAVNLAYGSAAGDAALVEVAARIRHFAHGELDGAWIAARGGGGQFLLLSTEPC
                     SRERWQMVGAQVLEALARPLALGGSTLRLSPRGALLRSLGGEDADSLLDRLGQALETV
                     MAAPGRRLSWADGEAIRAGRSAAQLEADLLRAIDLGEIEVVYQPQWSCADDRLTGAEA
                     LARWNHPRLGRIGAGALFAIAERADHVPQLSRHIAKTALAGARDWPDPLRLSLNVTPN
                     DLAEANFAAGFADLVAASGFPAARLTLEVTEQVLLADVAQAALALERLTGLGMRIALD
                     DFGAGFCNFRYLKLLPLHYLKLDRSMIEGAGDPRDLAVLRAIVAMAGALDLAVIAEGV
                     ETEAQRRLAAGEGCAFYQGFLRAQPLGAAEFRQLAG"
     gene            2674..3453
                     /locus_tag="VFV30_06125"
     CDS             2674..3453
                     /locus_tag="VFV30_06125"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX4847701.1"
                     /translation="MSKNPALALLASDTPRAQEAADHLRQDFAFVPLAEAEVAVVLGG
                     DGQMLHALHEMLDSDHVIPAYGVNYGTVGFMMNKPKSSRPLLERISRAKRLTITPLAM
                     TATLGSGKRVTGLAINEVSLLRETRQTARLEVSVNGKVRMAELFCDGVLVATPAGSTA
                     YNLSANGPILPLTSNLVALTPISPFRPRRWRGAILPDHYEMGFRVLDPDKRPVAVVAD
                     QKELRDILEVEVRVARDQQLTLLFDPGKTLEERIFAEQFQV"
     gene            3525..3599
                     /locus_tag="VFV30_06130"
     tRNA            3525..3599
                     /locus_tag="VFV30_06130"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3557..3559,aa:Asn,seq:gtt)
     gene            complement(3639..4379)
                     /locus_tag="VFV30_06135"
     CDS             complement(3639..4379)
                     /locus_tag="VFV30_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4847702.1"
                     /translation="MPDWLTPAFFGTALLYASVGFGGGSTYSALLSLAEFDFRLLPIV
                     SLCCNVLVVSGATLHFARAGVIPWKRALTLALVAAPLAFLGGLTPIREETFLLLLGAS
                     LVLAGLALFVPRAPDVAARAAPAARWMGLAAAPLGYLSGLVGIGGGIFLAPLLHLTRW
                     AGARQIAATTSLFILINSLAGLAGQLTKSGAERFGEALVHAVPLLVAVVLGGQLGSLL
                     ALRWLPEKVIRWLTAALTIWAGSRLILA"
     gene            complement(4387..>4764)
                     /locus_tag="VFV30_06140"
     CDS             complement(4387..>4764)
                     /locus_tag="VFV30_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX4847703.1"
                     /translation="AYAQKQPINEYKQEAFGLFEKMLEAIRIDVTKILMNSELRMPDP
                     VDLPELPDFLTSHIDPFTGENDAGARTPGAEAMLGAMAAGAALGAPPVAGEDPYAGMG
                     LSRNALCPCGSGQKYKHCHGAVG"
BASE COUNT          817 a         1683 c         1571 g          693 t
ORIGIN      
        1 cagttcaagc gccttggcct tgtaatcgtt gacgaagagc agcgctttgg cgtcaaccac
       61 aaggaagcgc tgaagcagct gcgcaacaac gtccacgtcc tcacccttac cgccacgccg
      121 atcccgcgca cgctgcagat ggcgatgagc gggctgcgcg agctgtccac gatccagacc
      181 gcgccggtcg accgcctggc ggtgcggacc tatgtgatgg aatgggacga catggtgatg
      241 cgcgaagccc tgctgcgcga acaccaccgc ggcgggcaga gcttcatcgt tgtcccccgg
      301 atcgccgaca tggccgaggt ggaggactgg ctgcacaaga ccgtgccgga agtgcgctat
      361 atctccgccc acggccagat ggccgccggc gaggtggaag agcggatgag cgccttctac
      421 gagaagaagt acgaagtgct gctgtccacc accatcgtcg agagtgggct ggatattccg
      481 agcgccaaca cgatcatcat ccaccgcgcc gaccggtttg gcctggccca gctctatcag
      541 ctgcgcgggc gggtcggccg ggccaagctg cgcgcctatg cctatctgac caccccggcg
      601 gatcggcagc tgtccgaagt cgccgaaaag cggctcaagg tgctgggcga tctcgacagc
      661 ctgggcgcgg gcttccagct tgccagccac gatctcgaca ttcgcggcgc gggcaacctg
      721 ctcggcgacg agcagagcgg tcatatccgc gaggtgggct tcgagctgta ccaatcgatg
      781 ctggaggacg cgatcctggc ggccaaggcc ggcgatcttg gcgtggtcaa ggaacgccac
      841 gcgctcagcc cgcagatcac ggtcgatgcg ccgatcatga tcccggaaga ctatgtgccc
      901 gatctggccg tgcggatggc gctctaccgc cgcctgaacg acgccgagga caccggcgag
      961 gtcgaagcgc tctcggccga gatgatcgac cggttcgggc ccctgcccca gccgaccgcc
     1021 aacctgatca agcttatcca gatcaagcgc caggcgatcg aggccaacat cgccaagatc
     1081 gatgtcgggc cgaaaggcac gctggtttcg ttccacaacg acgaatttcc ggatccggcg
     1141 ggcctgctca attacgccca gcgcttggaa gggacgatca agctgcgccc ggacagcaag
     1201 atcgtggtgg tccgcgcctg gggcagcgcc gaagcgcggc tcaacggcct gttccagctg
     1261 accaaggggc tcagcgcgat tgcgcgaaag gcgcggtagc ccaattcctc gcctttgggg
     1321 aggatcagcc tgccagttgc cggaactccg ccgcgccaag cggctgggcg cgcaggaagc
     1381 cctggtaaaa ggcgcagccc tcgcccgcgg caaggcggcg ctgggcttcg gtttcgacgc
     1441 cctcggcaat caccgccagg tccagcgccc cggccattgc cacgatcgcc cgcaggaccg
     1501 ccaggtcgcg cgggtcgccg gcgccttcga tcatgctgcg gtccagtttc aggtaatgga
     1561 gcggcagaag cttgaggtag cggaagttgc agaaccccgc cccgaaatcg tcgagcgcga
     1621 tccgcatgcc gagcccggtc agccgttcca gcgccagcgc ggcctgggcc acgtcggcca
     1681 gcaggacctg ttcggtcact tccagcgtca gccgcgcggc cggaaagccg ctggcggcaa
     1741 ccagatcggc aaacccggcg gcgaagttgg cttcggccag atcgttcggc gtgacattca
     1801 acgacaggcg cagcggatcg ggccagtccc gcgccccggc cagcgcggtc ttggcgatat
     1861 ggcgggagag ttgcggcacg tggtcggccc gttcggcaat cgcgaacagg gcgccggccc
     1921 cgatccggcc aagccgggga tggttccacc gcgccaatgc ctcggcgccg gtcagccggt
     1981 catcggcgca ggaccactgc ggctggtaga ccacctcgat ttcgcccaga tcgatcgcgc
     2041 ggagcaggtc ggcctccagc tgggccgcgc tgcgcccggc gcggatcgcc tcgccatcgg
     2101 cccagctgag ccgccgtccg ggggcggcca tgaccgtttc cagcgcctgc cccagccggt
     2161 cgagcagcga atccgcgtcc tctccgccca gcgaacgcag cagcgccccg cgcggagaca
     2221 gccgcagcgt gctaccaccc agcgccagcg gccgggccag cgcttccagc acctgggcgc
     2281 cgaccatctg ccagcgctcg cggctgcacg gttcggtgga aagcagcagg aactgcccgc
     2341 ccccgccgcg cgcggcgatc caggcgccgt ccagctcgcc gtgggcaaag tgccggatcc
     2401 gcgccgccac ctcgaccagc gcagcatcgc cggccgccga tccataggcc agattgaccg
     2461 catcgaaccg ccgcagaccg atcagcagcg cgtgaaccgg ctcgccccga tcgagccatt
     2521 cggccagccg cgcccgcgcg gcatcgcggc cgggcaggcc ggtcagcgcg tcgctgcgca
     2581 gcggcaaggg ttcggactgc agcgcatcca tcccgccgcc catagcacaa ggcccctacc
     2641 aaagtgttcc aattgcccat atgcaccagg ccgatgagca agaacccggc cctcgccctg
     2701 ctggcatcgg acactccgcg cgcgcaggag gcggccgacc acctgcgtca ggacttcgcc
     2761 ttcgtgcccc tggccgaggc cgaggttgcg gtggttctgg gcggcgacgg gcagatgctc
     2821 cacgcgctgc acgaaatgct cgattccgat cacgtgatcc cggcctatgg cgtcaactac
     2881 ggcacggtcg ggttcatgat gaacaagccg aagtccagcc gcccgctgct ggaacggatt
     2941 tcccgggcca agcgcctgac catcaccccg ctcgccatga ctgccaccct gggcagcggc
     3001 aagcgggtga cgggcctggc gatcaacgaa gtctcgctgc tgcgcgaaac ccgccagacc
     3061 gcgcggctgg aagtgtcggt caacggcaag gtgcgcatgg ccgaactgtt ctgcgacggc
     3121 gtgctggtgg cgaccccggc cgggtccacc gcctacaacc tctcggccaa cggcccgatc
     3181 ctgccgctga cctccaacct ggtcgcgctg accccgatca gtccgttccg cccgcgccgc
     3241 tggcgcgggg cgatcctgcc cgatcattac gagatgggct ttcgcgtgct cgatccggac
     3301 aagcgcccgg tcgccgttgt cgccgaccag aaggagctgc gcgacatcct ggaagtggaa
     3361 gtgcgggttg cccgcgacca gcagctgacc ctgctgttcg atcccggcaa gacgctggag
     3421 gagcggatct tcgccgaaca gttccaggtc tgattttttg gccagacccc gcttgccaaa
     3481 ccgtttcgcc ctgctatagg cgctcacctg cccgcaaggg ttgctccccg atagctcagc
     3541 ggtagagtag gtgactgtta atcacttggt cgtaggttcg aatcctactc ggggagccac
     3601 ttttcaaaag ccctgccgca aggcggggct ttttgctgtc aggccaggat aagccggctt
     3661 ccggcccaga tcgtcagcgc ggcggtgagc cagcggatca ccttttccgg cagccagcgc
     3721 agcgccagca ggctgcccag ctgaccgccc agcaccaccg cgaccagcag cggcacggca
     3781 tggaccagcg cttcgccaaa ccgttcggcg ccgctcttgg tcagctgccc ggccagcccg
     3841 gccagcgaat tgatcaggat gaacaggctg gtcgtcgccg cgatctgccg ggcgccggcc
     3901 cagcgggtca ggtgcagcag cggcgcgagg aagatcccgc cgccgatccc gaccagtccg
     3961 gagagatagc ccagcggcgc ggcggccagg cccatccagc gcgcggccgg agccgcccgc
     4021 gccgccacgt ccggcgcgcg cggcacgaac agcgccagcc cggccaggac caggctggct
     4081 cccagcagca gcaggaaggt ttcctcgcgg atcggggtca gcccgcccag gaaagcgagc
     4141 ggcgcggcga ccagcgccag ggtcagggcc cgtttccacg ggatcacccc ggcccgggcg
     4201 aagtgcagcg tcgctccgct gaccaccagg acgttgcagc acaggctgac gatcggcagc
     4261 aggcggaaat cgaactccgc caggctgagc agcgcgctgt aggtcgatcc gccgccgaaa
     4321 ccgacggagg cgtaaagcag cgccgtgccg aaaaaggctg gggtcagcca gtccggcacg
     4381 gcgggatcag cccactgccc cgtggcagtg cttgtacttc tggcccgaac cgcacgggca
     4441 cagcgcgttg cgcgacaggc ccatgccagc ataggggtct tccccggcca ccggcggcgc
     4501 gcccagtgcg gcaccggccg ccatggcccc cagcatcgct tccgcgcccg gggtccgcgc
     4561 accggcgtcg ttctcgccag tgaagggatc gatgtggctg gtcaggaagt cgggcagctc
     4621 gggcaggtcg accggatcgg gcatccgcag ctcgctgttc atcaggatct tggtcacgtc
     4681 gatccggatc gcctcgagca tcttttcgaa caggccgaag gcttcctgct tgtattcgtt
     4741 gatcggctgc ttctgcgcat aggc
//
LOCUS       DASUDV010000283         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_186056,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000283 DASUDV010000000
VERSION     DASUDV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186056"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFV30_06145"
     CDS             <1..505
                     /locus_tag="VFV30_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX4847704.1"
                     /translation="ASLLRTTPDEVEARVAALLDERRKLENELAGLKKERALQSSTFV
                     NQSTFFPATVTQEPPLVNGIKFTHLVLADLEPKELRSLIDEKKKDMGSGVAAIVTIFD
                     GKASIAVGVTDDLTGKVSAVDLVRAGVEALGGKGGGGRPDLAQGGGPDGSKATEAIAA
                     VKAVLAG"
     gene            complement(502..2283)
                     /locus_tag="VFV30_06150"
     CDS             complement(502..2283)
                     /locus_tag="VFV30_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4847705.1"
                     /translation="MAGPVELVPTSALSDALVILGAAGIVIPLFARLRITPVIGFILV
                     GLLVGPFGLGAAVNDQPWLAYVTISDPARLAPFAEFGIVLLLFSVGLELSFGRLWSMR
                     KQVFGLGSRELLGSALLIAIGLMVLGNSPPTALALGLALALSSTALVLRITDPRTPVG
                     KAALAMLLFEDIALVPIIFLLGAMGRGDAAQDAGDFVHTLWQGGLVVVALLVAGRFLL
                     PGLFGQAARTKNPELFLAASLLVVIASSLATSAVGLSPIVGALIAGLLIAETEYQGEV
                     EHITEPFKGLALGVFLITIGMSLNLDLLWTQFGEVALAVALVFGAKALVTGFLLKMSG
                     ARVGTATETGILLASPSETTLIVLTAALSARLISPDAAQFWQLVTALGLTITPILAIA
                     GRWLARRVDAVAYLDQAVDDPDQNRAIVVGCGRVGRLVADMLAEHGKPYVAVDFDIEQ
                     VRAAQGAGVNAVFGDAARGKALDRIGASHASAVILTMNEPVAAQRMVRSLRALYPDLP
                     IIARARDVSHAAALYRAGASHAVPETLESSLQLSEAVLVDLGVPMGPVIASIHEKRDE
                     FRQQIMHEGELAEMPKLKSASLRERIG"
BASE COUNT          464 a          777 c          801 g          358 t
ORIGIN      
        1 ggccagcctg ctgcgcacca cgcccgacga ggtcgaggcg cgcgttgccg cgctgctcga
       61 cgagcgccgc aagctcgaga acgagctggc ggggttgaag aaggaacgag cacttcaatc
      121 aagtacgttt gtcaaccagt ccaccttttt ccccgcgacg gtgacccaag agcccccttt
      181 ggtgaacgga attaaattca cacacctggt cttggctgac ctagagccaa aggaacttcg
      241 ctcgcttatc gacgagaaga agaaagatat gggttcgggt gttgcagcga ttgtcaccat
      301 ctttgatgga aaggcttcaa ttgcggtggg tgtcaccgat gacctgaccg gcaaggtcag
      361 cgcggtcgat ctggtccgcg ccggcgtcga agcgctgggc ggcaagggcg gtggtggccg
      421 ccccgacctg gcccagggcg gcggccccga cgggagcaag gcgaccgagg cgatcgcggc
      481 ggtcaaggcg gttttggcgg gctagccgat acgttcgcgc aagcttgcgg atttcagctt
      541 cggcatttcg gcgagttcgc cctcgtgcat gatttgctgg cggaactcgt cgcgcttctc
      601 gtggattgaa gcgattacgg ggcccatcgg tacgccaaga tcgaccagca cggcctcgga
      661 cagctggagc gagctttcca gcgtttccgg cacggcgtgg cttgccccgg cgcgatagag
      721 tgcggcggcg tggctgacgt cgcgggcgcg ggcaatgatc ggcagatcag ggtaaagcgc
      781 acgcagcgag cggaccatcc gctgggcggc gacaggctcg ttcatggtca ggattaccgc
      841 actggcatgg ctggcgccga tccggtcaag cgccttgccg cgcgcggcat cgccgaacac
      901 cgcattaacc ccggccccct gcgccgcgcg cacctgttct atatcgaaat cgaccgcgac
      961 atagggcttg ccgtgttcgg ccagcatatc tgccaccagc cgcccgacgc gcccgcagcc
     1021 caccacgatc gcgcggttct gatcgggatc gtcgaccgcc tgatcaagat aggccaccgc
     1081 atcgacccgc cgcgccagcc agcgtccggc aatcgccagg atcggggtga tggtgagccc
     1141 cagcgcggtc accagttgcc agaactgggc cgcgtccggg ctgatcagcc gggccgaaag
     1201 cgcggcggtg agcacgatca gggtggtttc ggatggactg gcgagcagga taccggtttc
     1261 ggtggccgtt ccgacgcgcg cgccgctcat cttcagcagg aagccggtga ccagggcctt
     1321 ggcgccgaac accagggcca ccgccagcgc aacctcgccg aactgggtcc agagcaggtc
     1381 gaggttcagg ctcatgccga tggtgatcag gaaaacgccc agcgccagcc ccttgaatgg
     1441 ttcggtgatg tgctcgacct cgccctgata ctcggtctcg gcgatcagca ggccggcgat
     1501 cagcgcgccg acaatcggcg aaaggccaac cgccgaggtg gcgagcgacg atgcgatcac
     1561 caccagcagg ctggccgcga ggaacagctc ggggttcttg gtccgcgcgg cctggccgaa
     1621 caggcctggc agcaggaacc gtccggcaac cagcaatgcc accacaacca gcccgccctg
     1681 ccacagggtg tgaacgaaat cgccggcatc ctgtgccgca tcgccgcgcc ccatcgcgcc
     1741 cagcaggaag atgatcggca ccagcgcgat atcctcgaac agcagcattg cgagtgctgc
     1801 cttgcccacc ggggtcctcg gatcggtaat gcgcagcacc agcgcggtcg aggacagggc
     1861 cagcgctagc cccagggcca gggcggtagg cggcgaattg cccagcacca tgaggccgat
     1921 cgcgatcagc agggcagagc cgagcagttc ccgcgaaccc agcccgaaga cctgcttgcg
     1981 catcgaccac aggcgcccga acgacagttc cagtcccacc gaaaacagca gcagcacgat
     2041 cccgaattcg gcaaagggcg cgagtctggc gggatcggag atcgtcacat aggccagcca
     2101 tggctgatcg ttcaccgccg cgccaagccc gaaggggcca accagcaggc caaccagaat
     2161 gaagccgatc accggggtga tccgcagccg cgcgaacagc gggatcacga tccccgccgc
     2221 gcccaggatc accagggcat cgctgagtgc ggaagttggg acaagttcga cggggccggc
     2281 catgcgcgca accctaaagg agggcgggcc ttcttgtcac ggggccgggc atgaaaaagg
     2341 cggcgccggg tgccggggcc gcctctttcg cgattgggtt gatcaatcga ggaagggtta
//
LOCUS       DASUDV010000284         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1069611,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000284 DASUDV010000000
VERSION     DASUDV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069611"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFV30_06155"
     CDS             complement(<1..332)
                     /locus_tag="VFV30_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX4847706.1"
                     /translation="MKHDHRSFAPAAAAAYRPDAGERIRRFVPMVRRLAWHVHGTGRA
                     GVEVEDLIQAGMVALTEAAQRHAGPGEDGFAAYAKLRVRGAMVDLIRRAVPLSRGGTE
                     KRRQLRDA"
     gene            complement(329..>2178)
                     /locus_tag="VFV30_06160"
     CDS             complement(329..>2178)
                     /locus_tag="VFV30_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX4847707.1"
                     /translation="QGGAAAGHVFEAVGAFLVGGNFAVGLFVFMILMIINMVVITKGA
                     GRVSEVSARFTLDALPGKQMAIDADLAAGLLSADEAKARRREVATEADFYGSMDGASK
                     FVKGDAIAALLILGVNIIAGFCLGMISHGLSAGEAAQVYITLAVGDALVAQVPALLLS
                     IAAAVIVTRVADSRDLAGQIGGQLANPATWLPVAAILAAIGLIPAMPQTIFLPAALVA
                     AWLWRRLAARAARPAPAAEEPPPAEPGRIALDEVSDHTLVTIELGFGLVHLVDDRRGS
                     PLVARITGVRKQLSQTFGFVVPQFRVRDSLDLAPNDYRILLGGVALGAGAIQPDRILA
                     IDAGDVRDGARLNGEATLDPSFGCPAVWIDLAQRDHAIAEGYLTVDASTVIATQLNQL
                     LGVRAPALFGPDELKAILDAVRDHSAGLVDAVTPQPLSLAALTRLFRTLLEDGIPIGH
                     PVPILSALALGLQQTSEHERLVDLVRAELGPLIVGRICAPGERLPVLTLDAQLEAAIV
                     TGMADPASGQPVIEPDLARSIGEYVAALIAERGASAPPPALVVQPRARRALAALLKLR
                     APACTVLSIAELPAAQPIEVVAVIGAPPPPAPGLPQPHILPAELEGMPA"
BASE COUNT          379 a          771 c          736 g          292 t
ORIGIN      
        1 ctggcatcgc gcagctgtcg gcgcttttcc gtacccccgc gcgaaagcgg cacggcgcgg
       61 cggatcagat cgaccatcgc cccgcggacc cgaagcttgg cataggcggc aaagccatcc
      121 tcgccagggc cggcgtgacg ctgcgccgct tcggtcagcg cgaccatccc ggcctggatc
      181 agatcctcga cctcgacccc ggcgcgtccg gtgccgtgca cgtgccaggc caggcgcctc
      241 accatcggca cgaagcggcg gattcgttcg cctgcatcgg ggcgataggc cgccgcggcg
      301 gcgggtgcga aactgcggtg atcgtgtttc atgcgggcat accttcaagt tcggcaggca
      361 ggatgtgggg ctgcggcagg cccggggccg gcggcggcgg cgcgccgatc acggcgacaa
      421 cctcgatcgg ctgcgcagcc ggcaattcgg cgatcgaaag cacggtgcag gccggcgcgc
      481 gcagcttgag cagcgccgcc agtgcgcggc gcgcgcgcgg ctgcaccacc agcgcgggcg
      541 gcggcgcgga tgccccgcgc tcggcaatga gtgcagcgac atattcgccg atcgagcggg
      601 cgagatccgg ttcgatcacc ggctggccgc tggccggatc ggccatcccg gttacgatcg
      661 ccgcctcgag ctgggcatcg agggtgagca ccggcaggcg ttcgccaggc gcacagatcc
      721 gcccgacgat cagcggcccg agttcggccc ggaccagatc gaccagccgc tcgtgctcgc
      781 ttgtctgttg aagcccgagc gccagcgccg aaaggatcgg caccggatgg ccaatcggga
      841 tgccgtcttc gagcagggtg cgaaacagcc gggtcagcgc ggcgagcgag aggggctggg
      901 gcgttaccgc atcgaccagt ccggccgaat gatcgcgcac cgcatcgaga atggccttca
      961 gctcatccgg cccgaacagc gccggggcgc gcacgccgag cagctggttg agctgggtgg
     1021 caatcacggt cgatgcatcg acggtcagat agccctcggc aatggcatga tcgcgctggg
     1081 cgagatcgat ccacaccgcc gggcagccga aactcggatc gagcgttgct tcgccattga
     1141 gccgcgcgcc atcgcgcacg tcgccggcat cgatcgccag gatccggtca ggctggatcg
     1201 cccccgcgcc cagcgccacc ccgcccaaca ggatgcgata gtcgttgggc gcgaggtcga
     1261 ggctgtcgcg caccctgaat tgcggaacca cgaagccgaa ggtctggctg agctgctttc
     1321 tgactccggt gatccgcgcc acgagcggcg aaccgcgccg gtcatcgacc aggtggacca
     1381 ggccaaagcc cagttcgatc gtcaccaggg tgtgatcgga cacttcatcg agcgcgatcc
     1441 ggcccggctc cgcgggcggc ggctcttcag ccgccggtgc gggccgcgcc gcacgcgccg
     1501 ccagcctgcg ccacagccac gccgcaacca gggcggcggg cagaaagatc gtctgcggca
     1561 tggccgggat cagcccgatc gccgccagga tcgccgcaac cggcagccag gtggcgggat
     1621 tggccagctg tccgccgatc tgcccggcga gatcccggct gtcggcaacg cgggtaacga
     1681 tcaccgccgc ggcgatcgag agcagcagcg ccggcacctg cgccaccagc gcatcgccca
     1741 cggcaagggt gatatagacc tgcgccgcct cacccgccga aaggccgtgg ctgatcatgc
     1801 caaggcagaa gccggcaatg atattgaccc ccaggatcag cagcgcggcg atcgcatcac
     1861 ccttcacgaa cttgctggcg ccgtccatcg aaccatagaa atcagcctcg gtcgccactt
     1921 cgcggcggcg cgccttggcc tcgtcggcgc tcagcaggcc ggcggccaga tcggcgtcga
     1981 tcgccatctg cttgccgggc agcgcatcaa gagtgaagcg cgccgaaact tccgaaacgc
     2041 gccccgcgcc cttggtgatc accaccatgt tgatgatcat caggatcatg aaaacgaaca
     2101 ggccaaccgc gaaattgccg ccaaccagga aggcaccaac ggcctcgaac acatggcccg
     2161 ccgccgcccc gccctgat
//
LOCUS       DASUDV010000285         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1732346,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000285 DASUDV010000000
VERSION     DASUDV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732346"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFV30_06165"
     CDS             <1..696
                     /locus_tag="VFV30_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4847708.1"
                     /translation="ELGGKSPNIVFDDAGLDAAVTRGVRHCFNNTGQSCNAPTRMLVE
                     QSAYDRAVDAARAAAAQVALGDPAEPGAHIGPVVSKAQYDKIQRLIETGMAEGAQLVC
                     GGPGRAEGFNRGYYVRPTIFAGVTNQMTIAREEIFGPVLVMIPFADEAEAVEIANDTP
                     YGLAAYIQSQDLERARRVARQIRAGSVHLNGAGQDYCSPFGGFKQSGNGREWGVWGLH
                     DFLELKVINGYGA"
     gene            693..1883
                     /locus_tag="VFV30_06170"
     CDS             693..1883
                     /locus_tag="VFV30_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX4847709.1"
                     /translation="MKLTGIQTFVVGNPPPHFGGRYFVFVKLTTDNGSTGLGEAYCVP
                     FSPHLVAQMCEDVFARYMEGQDPHDIEKMWRRVYSSGFTQHPDLTLMGVMSALEMACW
                     DIVGKEANQPVYKLLGGQVHEKLRAYTYIYPRPGDQTDVYHDPDLAAERAQLYLDMGF
                     TALKFDPAGPYSAFDGRQLSLEALDLSERFCRALREAVGTKADLLFGTHGQMTAAGAI
                     RLAKRLEPYDPLWLEEPIPPDAPEEMAKVARATSIPIATGERLTTKYDFARLLDCGAA
                     AILQMNLGRVGGLLEAKKIAGMAEVRHVQIAPHLYCGPVVGAANVQLATCSPNFLILE
                     SIEDWGGFHSQILKRGIQFEQGHVIPPTAPGLGIELDEEVALAHPYTGDMLHLEMTQH
                     PVSQ"
     gene            1880..>2569
                     /locus_tag="VFV30_06175"
     CDS             1880..>2569
                     /locus_tag="VFV30_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847710.1"
                     /translation="MSASYDYIVVGAGTAGCVVASRLADSGARVLLLEAGGSDRTLWI
                     QLPIGYGRTYFDPKVNWMYETEGVPGLDGRTSYWPRGKVVGGSGSINAMVHVRGQPHD
                     FDDWRDAGNPGWGWHDVLPHFIRAEDSDIAEPGWRGQGGPQHVTDISAKVHPLCHRFL
                     AAGQTLGLPLSPDFNGSQPEGLGLFQITTRNGRRASTANEYLRPALRRGRIDLQTHAR
                     ATAIRFDGKRAS"
BASE COUNT          437 a          887 c          852 g          393 t
ORIGIN      
        1 gaactgggcg ggaaatcgcc caacatcgtg ttcgacgatg ccgggctgga cgcggcggtg
       61 acgcggggcg tgcgccactg cttcaacaac accggccagt cctgcaacgc gccgacccgg
      121 atgctggtgg agcaatcggc ctatgaccgc gcggtcgacg cggcccgcgc ggccgccgcg
      181 caggtggcgc tgggcgatcc ggccgaaccg ggggcgcata tcggaccggt ggtcagcaag
      241 gcccagtacg acaagatcca gcggctgatc gaaaccggca tggccgaagg cgcgcagctg
      301 gtctgcggcg gccccggccg cgccgaaggg ttcaaccgcg gctactatgt ccgcccgacg
      361 atctttgccg gcgtgaccaa ccagatgacc atcgcccgcg aggagatctt cggcccggtg
      421 ctggtcatga tcccctttgc cgacgaggcc gaggcggtgg agatcgccaa cgacacccct
      481 tatggcctgg ccgcctatat ccagagccag gatttggagc gggcccgccg tgtcgcgcgc
      541 cagatccgcg ccggcagcgt ccacctcaac ggcgccgggc aggactattg ctcgcccttc
      601 ggaggcttca agcaatcggg caacggccgc gaatggggcg tgtggggtct gcacgacttc
      661 ctcgaactca aggtcatcaa cgggtacggc gcatgaagct taccggcatt cagaccttcg
      721 tggtcggcaa cccgcccccg catttcggcg ggcgctattt cgtgttcgtc aagctcacca
      781 ccgacaacgg ctcgaccggg ctgggcgagg cctattgcgt gcccttctcg ccccacctgg
      841 tggcgcagat gtgcgaggat gtcttcgccc gctacatgga agggcaggat ccccacgaca
      901 tcgagaagat gtggcggcgg gtctattcca gcggttttac ccagcatccg gacctgaccc
      961 tgatgggggt gatgagcgcg ctggaaatgg cctgctggga cattgtcggc aaggaagcga
     1021 accagccggt ctacaagctg ctgggcggcc aggtgcacga gaagctgcgc gcctatacct
     1081 acatctatcc gcgccccggc gaccagaccg acgtctacca cgatcccgac ctcgcggcgg
     1141 agcgggcgca gctctatctc gacatgggct tcaccgcgct gaagttcgat ccggcaggac
     1201 cctattcggc ctttgacgga cgccagctga gcctggaggc gctggacctt tcggagcggt
     1261 tctgccgtgc cttgcgcgaa gcggtcggca ccaaggccga tctgctgttc ggcacccacg
     1321 gccagatgac cgcggcgggc gcgatccggc tggccaagcg gctggaaccc tatgacccgc
     1381 tgtggctgga agaaccgatc ccgcctgatg cgcccgagga gatggccaag gtcgcccgcg
     1441 cgacctcgat cccgatcgcg actggcgagc ggctcaccac caagtacgat ttcgcccgcc
     1501 tgctcgattg cggcgcggcg gcgatcctgc agatgaacct gggccgggtt ggcggcctgc
     1561 tggaagccaa gaagatcgcc ggcatggccg aagtgcgcca cgtccagatc gccccgcacc
     1621 tctattgcgg cccggtggtc ggcgcggcca acgtccagct ggccacctgt tcgcccaact
     1681 tcctgatcct ggaaagcatc gaggactggg gcgggttcca ttcgcagatc ctgaagcgcg
     1741 ggatccagtt cgagcagggc cacgtgatcc cgccgaccgc gccgggcctg ggcatcgaac
     1801 tggacgagga ggtggccctg gcccacccct acaccggtga catgctgcac ctggaaatga
     1861 cgcagcaccc ggtcagccag tgagcgcttc ctacgactat atcgtggtcg gcgcgggcac
     1921 cgccggctgc gtggtggcaa gccggctggc cgacagcggc gcgcgcgtgc tgctgctgga
     1981 agccggcggg agcgaccgca cgctgtggat ccagctgccg atcggatacg gccgcaccta
     2041 tttcgatccc aaggtcaact ggatgtacga gaccgagggc gtgcccggac ttgacgggcg
     2101 cacgtcctac tggccgcggg gcaaggtggt gggcggatcc ggctcgatca acgccatggt
     2161 ccatgtccga ggccaaccgc acgatttcga tgactggcgc gatgccggca accccggctg
     2221 gggctggcac gatgtcctgc cccatttcat ccgcgccgaa gacagcgaca ttgccgaacc
     2281 cggctggcgc ggccagggcg ggccacagca cgtgaccgac atttcggcca aggtgcatcc
     2341 actctgccac cgcttcctgg cggcgggcca gacgctgggc ctgccgctca gccccgactt
     2401 caacggcagc cagccggaag gcctcggtct gttccagatc accacccgga acggccgccg
     2461 cgcctccacc gccaacgaat acctgcgccc ggcgctgcgc cgcggccgga tcgacctgca
     2521 gacccacgcc cgcgccaccg cgatccgctt tgacggcaag cgcgccagc
//
LOCUS       DASUDV010000286         9639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_936895,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000286 DASUDV010000000
VERSION     DASUDV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936895"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFV30_06180"
     CDS             <1..566
                     /locus_tag="VFV30_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX4847711.1"
                     /translation="VGNTVLYGAIAGEAFFNGVAGERFAVRNSGAVAVVEGCGDHGCE
                     YMTGGVVAVLGKTGRNFAAGMSGGIAYVYDPDGTFAQLCNPAQVDLLPISPERDEDDG
                     AGRPQQRPQSVYDNGMGDPLRHDAERLKVLLERHHLHTGSKRARALLDDWAGTLGKFV
                     KVMPRDYAKALKQLEAERLGAASVAAE"
     gene            578..2014
                     /locus_tag="VFV30_06185"
     CDS             578..2014
                     /locus_tag="VFV30_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX4847712.1"
                     /translation="MGKETGFLELDRQDRAYADPKERVQHYHEFVVPPAEPALKAQAS
                     RCMNCGIPYCHNGCPVNNIIPDWNHLVYEGDWQNALEVLHSTNNFPEFTGRICPAPCE
                     ASCTLNITDQPVTIKSIECAIVDKGWDEGWIVPQIPARRTGKSVAVVGSGPAGLAAAQ
                     QLARAGHSVTVFEKSDRVGGLLRYGIPDFKLEKHLINRRIVQMEAEGVQFRTSTEVGV
                     AVSVASLKENFDAIVLSGGAEDPRPLSIPGFELPGVRFAMEFLTQQNKRNAGDDEVRA
                     APRGSLLATGKHVVVIGGGDTGSDCVGTSIRQGAASVTQIEIMPKPPEKENKLLTWPD
                     WPMKLRTSSSHEEGCARDWAILTKRVVGKNSQVTGLECVRIEWVDGKMQEIPGSEFTL
                     QADLIFLAMGFLGPKKQGLLDQAGVALDPRGNVAADTASYRTSDPQIFACGDMRRGQS
                     LVVWAIREGRQAARAVDEALMGVSELPR"
     gene            complement(2040..2567)
                     /locus_tag="VFV30_06190"
     CDS             complement(2040..2567)
                     /locus_tag="VFV30_06190"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEX4847713.1"
                     /translation="MTRLILTYGMIAGTIELALLALSMGLVSDHGSLGMALGYLSMLI
                     AMSLVFVGVKRFRDEHQGGVIRFGKAFLVGLGIAGIACLFYVIGWELYMWATDHRFMA
                     EYMAKALAEKQASGASAADLAAFKAEMEGFAEMYRNPLLRMLITLSEIAPVGLLVPLI
                     SAAILRKSEVLPARA"
     gene            complement(2666..3076)
                     /locus_tag="VFV30_06195"
     CDS             complement(2666..3076)
                     /locus_tag="VFV30_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4847714.1"
                     /translation="MARLILSYGAALAALALLVEWLDWRHATRAWSTSAYVLCVALLF
                     AGLGIWLGNRLSPRPRGPYRRNEAALASLGISPREVEVLDLLAAGHSNKVIARQLDIS
                     PNTVKTHLAHLFEKLEAQSRTQAIARARALELVP"
     gene            complement(3109..3807)
                     /locus_tag="VFV30_06200"
     CDS             complement(3109..3807)
                     /locus_tag="VFV30_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX4847715.1"
                     /translation="MIELRPFSTLGAANHGWLDAHHHFSFASYHDPARVQWGALRVWN
                     DDAIAPKSGFPPHPHNDMEIITYVRQGAISHRDSLGNEGRTAAGDVQVMSAGTGIRHA
                     EFNLEDEECRLFQIWILPDRGGHAPSWGTRPFPREARDGRFTVLASGLPEDADALRIN
                     TSGRVAGAFVKAGETVRYETAPERHLYLVPATGRVRIGAVEAAARDGVAITGESAIEV
                     TALEDAELVLVDAA"
     gene            3927..4814
                     /locus_tag="VFV30_06205"
     CDS             3927..4814
                     /locus_tag="VFV30_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847716.1"
                     /translation="MLRIAFLACPDTLPGSPTRRPDAFEHDLLFDALNAGLAGRAGLM
                     GVDWRAPLDELAGFDLAYLGTPWDYTEAKDEFLERLAALEEAGVTVCNPVEVIRWNSD
                     KLYLRELDEGGAVSIPTLWPDQAGPAEVLAAFDHFGCDRVVVKRRVGAGAIGQDSFTR
                     ESPPPPGWRIDQPAMIQPFLPAIQSEGELSFIFVSGRLSHALVKQAAAGDYRIQSLYG
                     GREVPLTPAPADRAAAEAVMALLPFDQAPLYARIDMVRLDSGDLAVIEAELIEPYLYP
                     EQGPEFGSLLADAMLGRLG"
     gene            4830..7505
                     /locus_tag="VFV30_06210"
     CDS             4830..7505
                     /locus_tag="VFV30_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX4847717.1"
                     /translation="MLSRMSFGGQWRDYQQRVLEEFEGLIADRRLHVVAAPGSGKTVL
                     GLELIRRFGHPALVLAPSRTIRDQWAERLVPLFLPEPPGAEELSRDLAALRGLTCTTY
                     QALHAHWADDDGGARFAALVEQLKAHGALTLVLDEAHHLRREWWSALTALVDALPQAH
                     LVALTATPPYDAPAAEWARYDAMCGPIDLEIGAPELVRRGDLCPHQDHVLFSRPDQDA
                     LDLLEARRAWIAQLSADLRADGGFLTWLGVHPWLVEPDRHAEAILEAPELLSAVLVLL
                     ASAGRELPSAPLKLLGVKARDVPPPSAFWLEVLLNGFLFSHAETFRLGEERVKHLRSG
                     LSKHGLIEGSRVKLEESKRIFTLMAGSLAKLDSIAAIAAAEAAALGGALRLLVLSDHV
                     RAEELPRRPMADYRPAKLGVVPVFEVLRRSLPPGQRPGVLTGTLVILPDSAVPALHQL
                     GRARGVEPEDLRAAALPGCPGYARIEASGKGETALVGLVTALFQAGDITLLVGTQALL
                     GEGWDAPCVNSLVLASNSAAFMLSNQMRGRAIRIDPAAPDKVANIWHLASVETLPAGP
                     LPGLAQRLEWGRLDRGDGPTSDYDLLERRFRAFEGIANGESARITNGIERLGLWQAES
                     LDACNARTLALAADRAGTAARWQRSFGCAAPRAHVREVASPNYAPQRLAWGHTLSWLA
                     ISAGSAGALAGANELRTASTAGNLPVLAMAVAGAATLASLPRLAQAARLAWRNGSLES
                     SLTQVGQAVLHGLREAGAISDAQWHNAQLVVERDSSGVIDLLVEGVPRAAERTVLAAM
                     AEVLGPVGNPRYLLVRKSRLGLKHRTDYHAVPEAIGRQKAWAEAFHREWEQQVGPSDL
                     VFTRTGEGRIALLRARARSFAAGFQRRVDRRSAWR"
     gene            7558..8694
                     /locus_tag="VFV30_06215"
     CDS             7558..8694
                     /locus_tag="VFV30_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847718.1"
                     /translation="MTEPDDLAAFRAEVRTFLDESLTPELARAGRRATSVFAHRDHAL
                     AWQKILTARGWGAIDWPVEYGGTGWSPMQKYIFAAECARAGAPNLLPQGQKMVAPVLM
                     HYGTQEQKDYFLPRILSGEHYWCQGFSEPGSGSDLASLKLKAERDGDHYVLNGSKIWT
                     THAHFANWMFALVRTSSEGKPQAGISFLLIDMNTPGLKVDPIISLAGEHELNQVFFDN
                     VRVPVANRLGEENDGWSVAKYLLNYERGGRYAPGLFAQLAKLEDEAGDDPVLRERLRR
                     EAVTLEALEALEMKSIAAQTAGDGAKLALYASVLKVQGTEAAQRIDVLACEITGLAGW
                     ALADGQSPDLLATALPRYLNGRAATIYAGSNEIQRDLIAKVVLT"
     gene            complement(8691..>9639)
                     /locus_tag="VFV30_06220"
     CDS             complement(8691..>9639)
                     /locus_tag="VFV30_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847719.1"
                     /translation="TETATLLQQSLQRYLADHPRPAFAGLSELGLMAVGVPEEAGGVG
                     GGGIERAAIMADLGPALAGSDWLAHHLGASLLARLAPDHGLLAALAAGEARIALALGD
                     SGQVPLVAGAAEADWLVLAGEAQTLLVAADAPGLSRRHRAMMDGSVTADLSIGTGLDA
                     DTVLAEDADARAARDWAKDARDAALCAEAAGLMGRMVADTAAYLQQRKQFGVPIASFQ
                     ALRHRLADMQMAALKAEALTERAVLAEDGDGWARAVSAAVVETIDAVRVVGEGAVQLH
                     GGMGVTEELALGGQFKRGLAIAATLGSKSQHLARHAEQV"
BASE COUNT         1481 a         3368 c         3360 g         1430 t
ORIGIN      
        1 tcgtcggcaa caccgtgctt tacggcgcga tcgcgggtga ggcgttcttc aacggcgtgg
       61 ccggcgagcg ctttgcggtg cgcaattcgg gcgcggtcgc ggtggtcgag ggctgcggcg
      121 atcacggatg cgaatacatg accggcgggg tcgtcgccgt gctgggcaag accgggcgca
      181 actttgccgc cgggatgtcg ggcgggatcg cctatgtcta tgaccccgac ggcaccttcg
      241 cccagctgtg caacccggcc caggtcgatc tgctgccgat ttcgcccgag cgtgacgagg
      301 acgacggcgc cggccgcccg cagcagcgcc cgcaaagcgt ctatgacaac gggatgggcg
      361 atccgctgcg ccacgacgcc gaacggctga aggtgctgct ggaacgccac cacctccaca
      421 ccggttcgaa gcgcgcccgc gcgctgctgg atgactgggc cggaacgctt ggcaaattcg
      481 tcaaggtgat gccgcgcgac tatgcgaagg cgttgaagca actcgaggcc gaacggctgg
      541 gcgccgcttc ggtggccgcg gaatagggat caggaccatg ggcaaggaaa ccggctttct
      601 cgagcttgac cgccaggatc gcgcctatgc cgatcccaag gagcgggtgc agcactacca
      661 cgaattcgtg gtgccaccgg ccgaaccggc gctgaaggcg caggccagcc gctgcatgaa
      721 ctgcggcatc ccctattgtc acaacggctg tccggtgaac aacatcatcc cggactggaa
      781 ccacctggtc tatgaagggg actggcagaa cgcgctggag gtgctgcaca gcaccaacaa
      841 cttccccgag ttcaccggcc ggatctgccc cgcaccgtgc gaggcgagct gcacgctgaa
      901 catcaccgac cagccggtga cgatcaagag catcgaatgc gcgatcgtcg acaagggctg
      961 ggacgaaggc tggatcgtgc cgcagatccc ggcgcgccgc accggcaagt cggtggcggt
     1021 ggtcggctcc ggcccggccg gcctggccgc ggcccagcag ctggcccgcg ccggccattc
     1081 ggtgaccgtt ttcgaaaagt ccgaccgggt cggcgggctg ctgcgctatg gcatccccga
     1141 cttcaagctg gaaaagcacc tgatcaaccg ccggatcgtg cagatggagg ccgaaggcgt
     1201 ccagttccgg acctcgaccg aagtcggcgt ggcggtctcg gtcgcgagcc tcaaggaaaa
     1261 cttcgatgcc atcgtcctgt cgggcggggc agaggatccg cgcccgcttt cgatccctgg
     1321 gttcgaactg ccgggcgtgc gctttgcgat ggaattcctg acccagcaga acaagcgcaa
     1381 cgccggggac gacgaagtcc gcgccgcgcc gcgcggctcg ctgctggcca ccggcaagca
     1441 cgtggtggtg attggcggcg gcgacaccgg atcggactgc gtcggcacct cgatccgcca
     1501 gggcgcggcc tcggtcaccc agatcgagat catgcccaag cctcccgaaa aggagaacaa
     1561 gctgctgacc tggcccgact ggccgatgaa gctgcgcacc tcgtccagcc acgaagaagg
     1621 ctgcgcccgc gactgggcga tcctgaccaa gcgcgtcgtc ggcaagaaca gccaggtcac
     1681 cggtctggaa tgcgtccgga tcgaatgggt cgacggcaag atgcaggaga tcccgggcag
     1741 cgaattcacg ctccaggccg atctgatctt cctggccatg ggcttcctcg gccccaagaa
     1801 gcagggcctg ctcgaccagg ccggggttgc gctcgatccg cgcggcaacg tcgccgcgga
     1861 caccgccagc taccgcacca gcgacccgca gatcttcgcc tgcggcgaca tgcggcgcgg
     1921 gcaatcgctg gtcgtctggg cgatccgcga aggccgccag gccgcccgcg cggtggacga
     1981 ggcgctgatg ggcgtgagcg agctgccgcg ctaggcggca gcgcgccacc gcaacaggat
     2041 caggcccggg ccggcaggac ttcgctcttg cgcaggatcg cggccgagat cagcggcacc
     2101 agcagcccta ccggggcgat ctcgctcagc gtgatcagca tccgcagcag cgggttccgg
     2161 tacatttctg caaagccttc catctcggcc ttgaaggcgg cgaggtcggc cgccgaggcc
     2221 ccgctggcct gcttttcagc cagggccttg gccatgtatt cggccatgaa gcggtggtcg
     2281 gtcgcccaca tatagagctc ccagccaatc acgtagaaca ggcaggcaat cccggcgatg
     2341 ccgaggccga ccaggaaagc cttgccgaac cggatcaccc cgccctggtg ctcgtcgcgg
     2401 aagcgcttca cgcccacgaa aaccaggctc atcgcgatca gcatcgacag gtaacccagc
     2461 gccatgccga ggctgccgtg gtcgctgacc agccccatcg acagcgccag cagggccagc
     2521 tcgatggtgc cggcaatcat gccgtaagtc agaatcaggc gcgtcatagt cgtctctccc
     2581 cgtgttgcag agggctgccc agctatcgcc ccgcgccccg ccccgccatc gcccaaacgg
     2641 gtgaatcggc cccgatcacc cgcgtttacg gcaccagttc cagagcccgg gcccgggcaa
     2701 tcgcctgggt ccggctttgc gcctcaagct tttcgaagag gtgcgccagg tgggtcttga
     2761 ccgtgttggg cgagatgtcc agctgccgcg cgatcacctt gttggagtgg ccggcggcca
     2821 gcagatcaag cacttcgacc tcgcgcgggc tgatccccag gctagccagc gcggcttcgt
     2881 tgcggcggta gggcccgcgc ggccgcgggc tcagccggtt gcccagccag atacccagcc
     2941 ccgcgaacag cagcgccacg cacagcacat aggccgaagt gctccaggcg cgggtggcgt
     3001 ggcgccagtc gagccattcg accagcagcg ccagcgccgc aagggcggct ccataggaga
     3061 ggatcaggcg ggccatggca cgcgatggtg ccacgcccgc ccggcaaatc aagccgcgtc
     3121 gaccagcacc agctcggcgt cttccagcgc ggtcacttcg atcgcgcttt cgccggtgat
     3181 cgccaccccg tcgcgcgcgg ccgcctccac cgcgccgatc cgcacccgcc cggtggccgg
     3241 gacgaggtag agatgccgtt cgggtgcggt ctcgtaacgc accgtttccc cggccttgac
     3301 gaaggccccg gcaacccggc cgctggtgtt gatccgcagc gcgtcggcgt cttcgggcaa
     3361 gcccgaggcg agcacggtaa agcggccgtc gcgcgcttcg cgggggaaag gccgcgtccc
     3421 ccagctgggg gcatggccgc cgcgatcggg caggatccag atctggaaca ggcggcactc
     3481 ctcgtcctcc agattgaatt cggcgtggcg gatgccggtc cccgcgctca tcacctggac
     3541 atcgcccgcc gcggtgcggc cttcgttgcc caggctgtcg cggtggctga tcgcgccctg
     3601 gcggacatag gtgatgatct ccatgtcgtt gtggggatgg ggcggaaagc cgcttttcgg
     3661 ggcgatcgca tcgtcgttcc acacccgcaa ggcgccccac tgaacccgcg ccggatcgtg
     3721 gtagctggcg aaggagaaat ggtggtgcgc atccagccag ccgtggttgg ccgcgccaag
     3781 ggtggaaaag ggacgcaatt cgatcatcgg gtgtctccgc atccggccgc cgcgatcggc
     3841 acccgggtat tgcgcccaag atagttattg tctcggcccc cgccagatgg ttaccgggtg
     3901 gtcactgcct gtccaaagga tcacccgtgc tccgcatcgc ctttcttgcc tgccccgata
     3961 ccctgcccgg atcccccacc cgccgccccg atgcctttga gcacgacctg ctgttcgatg
     4021 cgctgaatgc ggggctggcc ggccgggccg ggctgatggg ggtggactgg cgcgcgccgc
     4081 tggacgaact cgccggcttc gatctggcct acctcggcac gccgtgggac tataccgagg
     4141 cgaaggacga attcctggag cggctcgccg cgctggaaga ggccggggtg acggtctgca
     4201 atccggtcga ggtgatccgc tggaattcgg acaagctcta cctcagggag ctggacgagg
     4261 gcggcgcggt ttctatcccc acgctctggc cggaccaggc ggggccggcc gaagtgctgg
     4321 ccgccttcga ccatttcggc tgcgaccggg tggtggtgaa gcgccgggtt ggcgccgggg
     4381 cgatcgggca ggacagcttc acccgcgaaa gcccgccccc gcccggctgg cggatcgacc
     4441 agccggcgat gatccagccg ttcctgcccg cgatccagag cgagggcgag ctgagcttca
     4501 tcttcgtctc cggccggctc agccacgcgc tggtcaagca ggccgccgcg ggcgattacc
     4561 gcatccagtc gctctacggc ggccgcgaag tgccgctgac ccccgctccg gctgaccggg
     4621 cggcggccga agcggtgatg gcgctgctgc cgttcgatca ggccccgctc tatgcccgga
     4681 tcgacatggt ccggctggac agcggggacc ttgccgtgat cgaggcggag ctgatcgagc
     4741 cctacctcta tcccgagcag gggcccgagt tcggcagcct gctggccgat gccatgctcg
     4801 gccggctggg atagagcggc gcggcgccga tgctctcgcg gatgtccttt ggcggccagt
     4861 ggcgcgacta tcagcagcgg gtgctggagg aattcgaagg gctgatcgcc gatcgccggc
     4921 tccacgtggt ggccgcgccc ggatccggca agaccgtgct ggggctggag ctgatccgcc
     4981 ggttcggcca tcccgccctg gtcctggccc ccagccgcac gattcgcgat caatgggccg
     5041 aacggctggt cccgctgttc ctgcccgaac cgcccggcgc ggaggagctg tcgcgcgatc
     5101 tggccgccct gcgcggcctg acctgcacca cctatcaggc gcttcacgcg cattgggccg
     5161 atgatgatgg cggcgcgcgg tttgccgcgc tggtcgagca gctgaaggcc cacggcgcgc
     5221 tgactctggt gctggacgag gcgcaccacc tgcgccgcga atggtggagc gcgctgactg
     5281 ccctggtcga tgccttgccc caggcccatc tggtcgcgct gaccgcgacc ccgccctatg
     5341 atgccccggc cgccgaatgg gcccgctatg atgcgatgtg cggcccgatc gatctggaaa
     5401 tcggcgcgcc cgaactggtc cggcgcggcg atctgtgccc gcaccaggac catgtgctgt
     5461 tttcccgccc cgatcaggat gcgctggacc tgctggaggc ccgccgcgcc tggatcgcac
     5521 agctttccgc cgatttgcgg gccgacggcg ggttcctcac ctggctgggc gttcatccct
     5581 ggctggtcga accggaccgt cacgccgaag cgatccttga agcgccggaa ttgctgtctg
     5641 ccgtgctggt cctgctggcc tcggccgggc gggagctccc gtcggccccg ctcaagctgc
     5701 tgggggtcaa ggcgcgcgac gtgcccccac cgtccgcctt ctggctggaa gtcctgctga
     5761 acggcttcct gttcagccat gccgagacct ttcgtctggg cgaggagcgg gttaagcact
     5821 tgcgcagcgg gctgagcaag cacggcctga tcgagggcag ccgggtcaag ctggaagaaa
     5881 gcaagcggat cttcacgctg atggccggga gcctggccaa gctggacagc atcgccgcca
     5941 tcgccgcggc cgaagccgcc gcgctgggcg gggcgctgcg cctgctggtc ctgtcggacc
     6001 atgtccgcgc cgaggaattg ccgcgccgcc ccatggccga ttaccgcccg gccaagctgg
     6061 gggtggtccc ggtcttcgaa gtgctgcgcc ggtcgctgcc cccggggcaa aggccgggcg
     6121 tgctgaccgg gaccctggtg attctgccag acagcgccgt gcccgcgctg caccaactgg
     6181 gccgcgcccg cggggtggag cccgaagatc tgcgcgccgc cgccctgccc ggctgccccg
     6241 gctatgcccg gatcgaggcc agcggcaagg gtgagaccgc gctggtgggg ctggtgacgg
     6301 cgctgttcca ggccggggac atcaccctgc tggtcggcac ccaggcgctg ctgggcgaag
     6361 gctgggacgc gccctgcgtc aacagcctgg tgctggccag caattcggcc gccttcatgc
     6421 tgtccaacca gatgcgcggg cgggcgatcc ggatcgatcc cgccgcgccg gacaaggtcg
     6481 ccaacatctg gcatctggct tcggtcgaga cgctgccggc cgggccgctg ccggggctgg
     6541 cgcagcggct ggaatggggc cggctcgatc gcggcgacgg cccgacgtcg gactacgacc
     6601 tgctggaacg ccggttccgc gccttcgaag ggatcgccaa cggcgaaagc gcgcggatca
     6661 ccaacgggat cgaacggctg ggcctgtggc aggccgaaag ccttgatgcc tgcaacgccc
     6721 gtaccctggc gctggcggcc gaccgggccg ggacggcagc gcgctggcag cgctcgttcg
     6781 gctgcgccgc gccgcgggcc catgtccgcg aagtggccag tcccaattac gccccgcagc
     6841 ggctggcctg gggccacacg ctgagctggc tggcgatcag cgcgggcagc gccggagccc
     6901 tggcgggggc caacgagctg cgcaccgcga gcacggccgg caacctgccg gtgctggcca
     6961 tggccgtggc cggtgccgcc acgctggcca gcctgcccag gctggcccag gccgcgcgcc
     7021 tcgcctggcg caacggatca ctcgaatcga gcctgacgca agtgggccag gcggtgctgc
     7081 acggactgcg ggaggccggc gcgatcagcg acgcgcagtg gcacaacgcg cagctggtgg
     7141 tcgaacgcga tagctccggg gtcatcgatc tgctggtcga aggcgtgccg cgcgcggccg
     7201 agcggacggt cctggcggcc atggccgaag tgctggggcc ggtcggcaac ccgcgctacc
     7261 tgctggtccg caagtcgcgc ctcgggctga agcaccggac cgattaccac gccgtgcccg
     7321 aagcgatcgg gcggcagaag gcctgggccg aagcgttcca ccgcgaatgg gagcagcagg
     7381 tcggtccgtc ggacctggtc ttcactcgca ccggcgaagg ccggatcgcc ctgctccgcg
     7441 cccgggcccg ctcgttcgcc gccgggttcc agcggcgggt cgaccggcgt tcggcctggc
     7501 gctgaaattc cgcagccggg ccttgaacca gcgggccagt gcgggcactg ttcgcccatg
     7561 accgaacccg acgatcttgc cgccttccgc gctgaagtgc gtacctttct ggacgagagc
     7621 ctgacgcccg agctggcgcg ggccgggcgg cgggcgacgt cggtctttgc ccaccgcgac
     7681 catgccctgg cctggcagaa gatcctgacc gcgcgcgggt ggggggcgat cgactggccg
     7741 gtggaatacg gcggcaccgg gtggagcccg atgcagaagt acatcttcgc ggccgaatgc
     7801 gcccgcgccg gcgcgcccaa cctgttgccg caagggcaga aaatggtcgc cccggtgctg
     7861 atgcattacg gcacgcaaga gcagaaggat tacttcctgc cccggatcct gtcgggcgag
     7921 cattactggt gccagggctt ttcggagccg ggttcgggat cggacctcgc ctcgctcaag
     7981 ctcaaggccg agcgcgacgg ggaccactat gtcctcaacg gctctaagat ctggaccacc
     8041 cacgcccact tcgccaactg gatgttcgcg ctggtccgca ccagcagcga gggcaagccg
     8101 caggccggga tctcgttcct gctgatcgac atgaacaccc cggggctgaa ggtcgacccg
     8161 atcatttcgc tggccggaga gcacgagctg aaccaggtgt tcttcgacaa tgtccgggtc
     8221 ccggtggcga accggctggg cgaagagaac gacggctggt cggtcgccaa gtacctgctc
     8281 aattacgagc gcggcgggcg ctatgccccg ggcctgttcg cccagctggc caagctggag
     8341 gacgaggccg gggacgatcc ggtgctgcgc gaacggctgc ggcgcgaggc ggtgacgctg
     8401 gaagcgctgg aagcgctgga gatgaaatcg atcgccgcgc agaccgccgg ggatggcgcc
     8461 aaactggcgc tctatgcctc ggtcctgaaa gtccagggca ccgaggcggc gcagcggatc
     8521 gacgtgctgg cctgcgaaat caccggcctg gccggctggg ccctggccga cgggcagagc
     8581 ccggacctgc tggccaccgc gctgccgcgc tatctcaacg gccgggccgc gacgatctat
     8641 gccggatcga acgagatcca gcgcgacctg atcgcgaaag tggtgctgac ctagacctgc
     8701 tcggcgtgac gggccaggtg ctggctcttt gaccccagcg tcgcggcgat tgccaggccg
     8761 cgcttgaact ggccgcccag cgccagttcc tcggtcacgc ccattccgcc gtggagctgg
     8821 accgcgcctt cgccgaccac ccgcaccgcg tcgatcgtct cgaccacggc ggcgctgacc
     8881 gcccgggccc agccgtcgcc gtcttcagcc agcacggcgc gctcggtcag ggcctcggcc
     8941 ttgagcgcgg ccatctgcat gtcggcgagg cggtggcgca gcgcctggaa gctggcaatc
     9001 ggcacgccga actgcttgcg ctgctgcagg taggccgcgg tatcggccac catccggccc
     9061 atcagcccgg ccgcctcggc gcagagcgcg gcatcgcggg catccttggc ccagtcccgc
     9121 gcggcgcggg catcggcgtc ctcggccagc acggtgtcgg cgtccagccc ggtcccgatg
     9181 ctgagatcgg cggtgaccga gccgtccatc atcgcccggt gccggcgcga caggcccggg
     9241 gcatcggccg ccaccagcag cgtctgcgcc tcgcccgcca ggaccagcca gtccgcctcg
     9301 gccgcgcccg cgaccagcgg cacctggccg ctatcgccca gtgccagcgc gatccgcgcc
     9361 tctccggcgg ccagcgcggc cagcaggccg tggtctggcg ccaaccgcgc gagcaggctg
     9421 gcgccgaggt ggtgggccag ccagtccgac ccggccagcg ccgggcccag atcggccatg
     9481 atcgccgccc gctcgatccc gccgccgcca accccgccgg cctcttccgg cacgcccacg
     9541 gccatcaggc ccagttcgga caggcccgca aaggccggcc gggggtgatc ggccagatag
     9601 cgctgaagcg actgctggag cagggtcgcg gtctcggtc
//
LOCUS       DASUDV010000287         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_31477,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000287 DASUDV010000000
VERSION     DASUDV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31477"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFV30_06225"
     CDS             <1..571
                     /locus_tag="VFV30_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HEX4847720.1"
                     /translation="WPGKNPPEHEANIVFTRMLEGPMDFTPGILSLKGSDDSNILSTI
                     SKQLALYVVIYSPVQMVADTPENYAKYPGPMKFIRDVPTDWQDTRVLNGEIGDYVTIV
                     RKERGGEDWYLGAVGDEEARTVSFQLDFLTPGTRYVAEIYRDGDDADYRTDKRHSIVI
                     EQRPVTSADSMTLRLAPGGGAAVRFRRVK"
     gene            568..1488
                     /locus_tag="VFV30_06230"
     CDS             568..1488
                     /locus_tag="VFV30_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4847721.1"
                     /translation="MIRRLLAVWAALLALVLAATPAAAGAENGRFVEVERMAAPGLPD
                     QRLTIWLPPGYDAGKRRYPVLYMHDGHNLFDPAKSNFNKVWAADKAMLDLIRAGTVEP
                     HIIVGVWAPGKDRYRQYLPKTAYLAASPALRAVMDGLAGGPIVSDRYLAWLAGPLKSW
                     VDGKFRTRRGPRDTAIAGSSMGGLMSCYAFVERPDVFGRAACVSTHWPMVMPGEREQA
                     DPERIAVHKAWFLDRLGRPAGRRLWLDIGTGTLDAFYPPYQAAVEQGIARTPWKRGRD
                     WQGTTYEGAEHEENAWARRLPEVLGWLLRK"
     gene            complement(1492..1971)
                     /locus_tag="VFV30_06235"
     CDS             complement(1492..1971)
                     /locus_tag="VFV30_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX4847722.1"
                     /translation="MNGQTAQLDSIDWAILEVLQADAALAVHEVGDRVGLSSNACWRR
                     IRRLEDSGIIARRVALLDPEKLGLATTVFVAIRTNRHDPQWLEAFSQGVAAIPEISEC
                     HRMAGDVDYLLKIVVRDIAHYDRIYRRLIAAVPDIADVSSSFSMEKMKATTALPRPA"
     gene            2071..2316
                     /locus_tag="VFV30_06240"
     CDS             2071..2316
                     /locus_tag="VFV30_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HEX4847723.1"
                     /translation="MNSSKLFTEHPASVGETYGEHLVMAGGFGIRMILGGIACLIHGL
                     LPFLFVKTGSAQISELHDVMVKNRQRGQMPAVLDFVI"
BASE COUNT          392 a          777 c          779 g          379 t
ORIGIN      
        1 ctggccgggc aagaacccgc ccgagcacga agccaacatc gtcttcaccc ggatgctgga
       61 agggccgatg gatttcaccc cgggcatcct cagcctcaag ggctcggacg acagcaacat
      121 cctctcgacc atttccaagc agctggcgct ttacgtggtg atctattcgc cggtgcagat
      181 ggtggccgac acgccggaaa actatgccaa gtatcccggc ccgatgaagt tcatccgcga
      241 cgtgccgacc gactggcagg acacccgcgt cctcaacggt gaaatcggcg actatgtcac
      301 catcgtccgc aaggagcgcg gcggcgagga ttggtacctg ggcgcggtcg gcgatgaaga
      361 ggcccgcacg gtcagcttcc agctcgactt cctgaccccc ggcacccgct atgtcgccga
      421 gatctaccgc gacggcgacg atgccgatta ccgcaccgac aagcgccatt cgatcgtgat
      481 cgagcagcgc ccggtgacca gcgccgacag catgaccctg cgcctcgccc ccggcggcgg
      541 tgcggcggtc cggttccggc gggtcaagtg atccggcggc tgctggcggt ctgggcggcg
      601 ctgctcgccc tggtgctggc agccaccccg gccgcggccg gggccgaaaa cggccgcttt
      661 gtcgaagtgg agcggatggc cgcgccgggc cttcccgacc agcggctgac gatctggctg
      721 ccgcctggct atgacgcggg caagcgccgc tacccggtgc tctacatgca cgacgggcac
      781 aacctgttcg atccggccaa gtccaatttc aacaaggtct gggccgccga caaggcgatg
      841 ctggacctga tccgcgccgg cacggtggag ccgcacatca tcgtcggggt ctgggcgccg
      901 ggcaaggacc gctaccgcca gtacctgccc aagaccgcct accttgccgc ctcgcccgcc
      961 ttgcgcgcgg tcatggatgg cctggccggc gggccgattg tctcggaccg ctacctcgcc
     1021 tggctggctg ggccgctgaa gagctgggtc gacggcaagt tccgcacccg ccgcggcccg
     1081 cgcgacaccg ccattgccgg atcgagcatg ggcgggctga tgagctgcta tgcctttgtc
     1141 gagcggcccg acgtgttcgg ccgcgcggct tgcgtttcaa cccattggcc gatggtcatg
     1201 ccgggcgagc gcgagcaggc cgatcccgaa cggatcgcgg tgcacaaggc gtggttcctg
     1261 gatcggctgg gccggcccgc cgggcggcgg ctgtggctcg atatcggcac cggcaccctc
     1321 gatgccttct acccgcccta tcaggcggcg gtggagcagg ggatcgcccg cacgccatgg
     1381 aagcgcggac gcgactggca aggcacgacc tatgagggcg ccgagcacga agagaacgcc
     1441 tgggcccgcc gcctgcccga agtgctcggc tggctgctgc gcaaatagcc gtcaggccgg
     1501 gcgcggcagg gcggtggtgg ccttcatctt ttccatcgag aagctggaac tgacatcggc
     1561 gatgtccggc accgcggcga tcagccggcg gtagatccgg tcgtaatggg caatgtcgcg
     1621 caccacgatc ttcagaaggt aatcgacatc gccggccatg cggtggcact cgctgatctc
     1681 gggaatcgcg gccacgccct ggctgaaggc ctccagccat tgcggatcat ggcggttggt
     1741 gcggatcgcg acgaacaccg tggttgccag gcccagcttt tcgggatcga gcagggccac
     1801 ccggcgggcg atgatcccgc tgtcctccag ccgccggatc cgccgccagc aggcgttgct
     1861 ggacaggcct acccggtcgc ccacctcgtg cacggccagg gcggcgtcgg cctgcaggac
     1921 ctccaggatc gcccagtcga tcgaatccaa ttgcgcggtt tggccgttca ttgggataaa
     1981 ctaatcgcct tggcggcaaa tagctagaaa aacggtgatg caattgccgc taggtcgggt
     2041 cataattgcg gccagacagg gagaagccgc atgaattcgt cgaagctgtt taccgaacac
     2101 cccgccagcg tgggcgaaac ctatggcgag catctggtca tggccggcgg gttcgggatc
     2161 cggatgatcc tgggcgggat cgcttgcctt atccacggcc tgctgccgtt cctgttcgtg
     2221 aagaccggca gcgcccagat ttcggagctg cacgacgtga tggtcaagaa ccggcagcgc
     2281 gggcagatgc ccgccgtgct ggacttcgtg atctagccca gcagcgc
//
LOCUS       DASUDV010000288         9027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2041973,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000288 DASUDV010000000
VERSION     DASUDV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041973"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFV30_06245"
     CDS             complement(<1..623)
                     /locus_tag="VFV30_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX4847724.1"
                     /translation="MRKLPATLSALALAFSLTGLVAVSQAPAATDAALPASEDARLTA
                     FLDGEFAELLKQQPQLATRLGLKEGGDRWNDTSDAAAEALVAWRKASVARMKAQFDRA
                     KLSPEGQVNYDIWELEAERAALSQANRIYRPPFYSRLYSAHSQLPDFLINTHTVADET
                     DLKNYIARLRTIPAVLDEAIRRSKVSDAAGIRAPRFQVETVIAGSEKL"
     gene            750..1304
                     /locus_tag="VFV30_06250"
     CDS             750..1304
                     /locus_tag="VFV30_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4847725.1"
                     /translation="MALILFNKPMNVLSQFTDKGTAGSARATLSDYIAVPGVYPAGRL
                     DRDSEGLLLLTDDGRLQARIADPKFKLPKTYLVQVEGDIGDAALEQLRRGVALSDGMT
                     RPAAAEAIAPPELWPRDPPVRFRKSVPDGWIRLTIHEGRNRQVRRMTAAVGLPTLRLV
                     RWAVGDWTLDGLAPGAWRAAGARP"
     gene            1277..2110
                     /locus_tag="VFV30_06255"
     CDS             1277..2110
                     /locus_tag="VFV30_06255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4847726.1"
                     /translation="MARSRGAAVNPDDVVSLGPLVLALDRVVALGLFALFLWAIDWIV
                     RRHGAGGASLGWWALAAGLIAARLDYVAVHRDSFALEPLEALRVWLGGWTWFSGVIAA
                     LAVLGARLGRTRAMAEAAGVLGLLALVWGGFAENRAAPTPLRLPPDLVFQRLDGTPLR
                     LDELRGQPVILNLWATWCPPCRRETPMLSRAASAAGAAPILLVNQGEERAAVQRYLAA
                     EGLPGQAFVLDPSGTLTTLTGSRALPTTLLLDEEGTVQAVHLGEISRVQIDILRRRAQ
                     R"
     gene            complement(2340..3647)
                     /locus_tag="VFV30_06260"
     CDS             complement(2340..3647)
                     /locus_tag="VFV30_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4847727.1"
                     /translation="MSAAIGPVASPEDGAAVIDPKSYADWDAALDLFDRLRAETPVVR
                     IESPTGEHDPFWLLTRHEDALRVSKDNVRFLNAPRPTVFTNKEGERLARDVTAKQGDE
                     SPNLVSSLVALDAPKHPKLRRLTQDWFMPKNLKTIEAAVRELAGKTADRLIAAAAENG
                     GEADFVPLVAAPYPLHVVMQILGVPEEDEPKMLFLTQQMFGGQDEDLSKSGMAQMTPE
                     MISQIVFGAVAEFETYFEGLAAERRKNPTEDVATILANADISARDRAGYYIIVAAAGH
                     DTTSASTAGAMLALAKDPDQFALVKADRSLVPGIVEEAIRWTTPVQHFMRTAMEDAQF
                     GDVTIKAGDWLMINYVAANHDPAQFDNPRKFDATREANRHLAFGAGTHQCLGLHLARL
                     EMRLLFEALLDRIDSVELAGEPKRAKSTFVGGLKTLPLRIKAA"
     gene            complement(3644..5197)
                     /locus_tag="VFV30_06265"
     CDS             complement(3644..5197)
                     /locus_tag="VFV30_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX4847728.1"
                     /translation="MHPSIHAKNQPDRPAIIMAGSGETITFAELDRRSNQVAQLLRSR
                     GIQVGDTVALCMENHPWFFCLTWGFQRSGVHYVGISSRLTPPEIAYILEDSGAKILFG
                     SAYLAPTLDEVAKLAPQVEQLRFDTPGALSAEDALAAMPESPIADERAGVDMLYSSGT
                     TGRPKGVKIPLPEDPAIDQANALVGLTMMAFGIKPDSVYLSPAPLYHAAPLRWSMTIQ
                     KLGGTVVVMEKFDPENALAAIEKYKITDSQFVPTHFVRMLKLPEDVRKKYDVSTLKCA
                     IHAAAPCPVPVKQAMIEWWGPVIYEYYAGTEGNGFTFVTSPEWLQKPGTVGKALTGII
                     RVCDESGDEVPRGTEGQIFFEPTEGMVPFEYHNDPQKTADARNKHGWTSLGDVGYQDE
                     DGYLFLTDRKSFMIISGGVNIYPQEIENLLVTHPKVADAAVIGAPDPDFGEKVVAVVQ
                     PLNMADAGPALAEELTEFLAPQLSRVKMPRQIDFREQLPRELTGKLYKRLLRDEYKEA
                     YEKAQGGAA"
     gene            5443..6933
                     /locus_tag="VFV30_06270"
     CDS             5443..6933
                     /locus_tag="VFV30_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4847729.1"
                     /translation="MEKPRQSWAGLANISFGFFGIQIGFALQNANMSRIFQTLGANMD
                     DLAWLWAAAPLTGLLVQPIIGHMSDKTWLGKLGRRRPYFLAGAVLAALSLFLMPISPM
                     LMMAAMLLWMLDASLNISMEPFRAFVGDMLRKDQHTAGYAVQTAFIGLGAVVGSLFPW
                     LLDHWGVANEAAPGVIPDTVRYSFWAGGAALFAAVMWTVFSTSEYSPEQMAGFGEAAE
                     SGAPSGANLAGRDSGGPFAWIMAGAALMVVTHQFALEKEVYLLSALLIGYGLISHIAI
                     RLVRAGRSAGMLTAIVGDFSGMPDIMKRLAVVQFFSWSALFTMWIYTTGIVAQRSFGT
                     TDKASAAFQEAGNWVGVLFSVYNGVAAVAALTLLPFLARRFGKARTHMFGLLCGAAGY
                     ASFFVITDPQWLILSEVGVGIAWASILAMPYAILASSLPQAKLGIYMGLFNVFVVVPQ
                     LLVATVMGTIMKHFFPGDPIWTMAFAAGTLVVAALAMTRVKVPDEA"
     gene            complement(6930..8873)
                     /locus_tag="VFV30_06275"
     CDS             complement(6930..8873)
                     /locus_tag="VFV30_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847730.1"
                     /translation="MALVLALLPQAVLVLALLPQAALAQRADENAATQSSDAFGRSVG
                     SDRSGLYSSDDVRGFDPVDAGNVRLEGLYYDQVDRLSPRLSDGSTIRVGPAGLRYPFP
                     APTGLVDFSLTQPRGATSYSFNLELAGNNAAGPGGSFEFKQPIDGSRLGISGGFGFRN
                     ARRWEGGDALYRTFGATLAWRPTDQISVAVFGGAFLFRSEEAKPIYYPSGTTPPPEAP
                     RGTDLTQPWTPRNAANWAYGGIVKAPLGPFRLEAGLFYNGRDQRSIFSDLYVGVNQDG
                     SVNARRVVADGNNRDASLSGEVRLVRQWQTGGINQRLTFSLRGRSRDRRFGGSQSIAL
                     GPSTILTRDVRPEPVYTIGPKNEDRIRQLTYGASWSVVGEGRFGLDVSLSKTAYRKTT
                     DFADPLLADQIARDGPLVWNVTGTVTLAPGLAVYGGYSTGLEEALAAPDVAINRAEAP
                     PAVRTRQFEAGLKYAVTPRLTLIAGAFSITKPYYNLDPALRYRQLGSLRNRGLEFSLT
                     GQIVPGLTVVGGTLLLDPEISGEAVRSGLIGGRPVGQVRRRSVLTLDWRSGHGKGPLS
                     FDLAVESLSSRAGNAANTLFAPPRTAVNLGARYRFKAAGGTWLVRPVLLNVFDNYGWN
                     VSSSGGFTYTTPRAVTLQLVADF"
BASE COUNT         1297 a         2996 c         3194 g         1540 t
ORIGIN      
        1 agcttctcgc tgccggcaat caccgtttcg acctgaaagc gcggggcgcg gatcccggcg
       61 gcatcgctga ccttgctgcg ccgaatcgcc tcgtccagca ccgccggtat ggtccgcagc
      121 cgggcgatgt agttcttgag gtcggtctca tcggccacgg tgtgggtgtt gatcaggaaa
      181 tcgggcagct ggctgtgcgc cgaatagagc cgcgaataga acggcgggcg atagatccgg
      241 ttggcctgcg acagcgcggc gcgctccgcc tccagctccc agatgtcgta attgacctgg
      301 ccttcggggg agagcttggc ccggtcgaac tgcgccttca tccgcgcgac gctggccttg
      361 cgccaggcca ccagcgcttc ggcggcggca tcgctggtgt cgttccagcg gtcgccgccc
      421 tctttcaggc ccagccgggt agcaagctgc ggctgctgct tcagcagctc ggcaaattcg
      481 ccgtcgagga aggcggtcag ccgcgcgtcc tcgctggccg gcagggcggc atcggtggcg
      541 gccggggcct ggctgacggc aaccagcccg gtcaggctga acgcaagggc gagggcggaa
      601 agggtggcgg ggagcttgcg catgggcatg attccttgtg tcttcgcagc gatccttagc
      661 aggcgctcgc cccgggtgaa gtccccgaaa ggacaggtgc gccgcattcc ccgctttcgc
      721 gcgcccgcgg cctcgcctaa gccggcgtca tggcgctgat cctgttcaac aagccgatga
      781 acgtcctctc gcagttcacc gacaagggga cggcgggttc ggcgcgggcg acgctgtcgg
      841 actatatcgc ggtgccgggg gtctatcccg ccgggcggct cgaccgggac agcgaggggc
      901 tgttgctatt gaccgacgac ggacggctgc aggcgcggat tgccgatccg aagttcaagc
      961 tgcccaagac ctatctggtc caggtggaag gggacatcgg cgatgcggcg ctggagcagc
     1021 tgcgccgggg cgtggcgctc agcgacggga tgacccgccc ggccgcagcc gaggcgatcg
     1081 cgccgccgga gctgtggccg cgcgatccgc cagtgcggtt tcgcaagtcg gtgcccgatg
     1141 gctggatccg cctgaccatc cacgaaggcc gcaaccgcca ggtccggcgg atgaccgctg
     1201 cggtcggcct gcccacactg cggctggtcc gctgggcggt gggggactgg acgctggacg
     1261 gactggctcc gggcgcgtgg cgcgcagccg gggcgcggcc gtgaacccgg acgacgtcgt
     1321 ttcgctgggg ccgctggtgc tggcgctcga ccgggtggtc gcgctcggcc tgttcgcgct
     1381 gttcctctgg gcgatcgact ggatcgtgcg ccgccacggc gccggcgggg ccagcctggg
     1441 ctggtgggcg ctcgccgccg ggctgatcgc ggcgcggctg gactatgtgg cggtgcaccg
     1501 ggacagcttc gcgctggaac cgctcgaagc cttgcgggtc tggctgggcg ggtggacctg
     1561 gttcagcggg gtcattgcgg cgctggccgt gctcggcgcc cggctcggcc gcacgcgcgc
     1621 gatggccgag gccgccggcg tgctcggcct gctggcactg gtctggggcg gctttgcgga
     1681 gaaccgggcc gctccaacgc ccctgcggct gccgcccgat ctggtgtttc agcggctgga
     1741 cggcaccccg ctgcggctcg acgagctgcg cggtcagccg gtgatcctca acctctgggc
     1801 gacctggtgt ccgccctgcc gccgcgaaac cccgatgctg agccgcgccg cgtcagcggc
     1861 gggagcagcg ccgatcctgc tcgtcaacca gggcgaagag cgcgccgcgg tgcagcgcta
     1921 tctcgcggcg gaagggctgc cggggcaggc cttcgtgctc gatccgtccg gcacgctgac
     1981 caccctcacc ggcagccgcg cgctgccgac cacgctgctg ctcgatgagg agggcaccgt
     2041 ccaggcggtc cacctgggtg agatcagccg cgtgcagatc gatatcctgc gccgccgcgc
     2101 acagcgctag gccctgtcac agcgccgcaa catcgcggcg ctacgaatcg gggcatggac
     2161 caagcacccg cccaattcga ctggatcggc tggctcgcca cgatccccgg aatccctgcc
     2221 ggctggggcg ttgccccggc cgaccccgcg cccgcgttcg cggccccttc ggcggagtcc
     2281 ctcccgcccc gggccgcggc gcgggttgcg gagcctcacg cgggtgaccg cctcgccgct
     2341 taggcggcct tgatccgcag cggcagggtc ttcagcccgc cgacgaaggt gctcttggcg
     2401 cgcttcggtt cgccggccag ttcgacgctg tcgatccggt cgagcagcgc ttcgaacagc
     2461 agccgcattt ccagccgcgc caggtgcagg cccaggcact ggtgggtgcc cgcgccgaag
     2521 gcgaggtggc ggttcgcctc gcgggtggcg tcgaacttgc gcggattgtc gaactgcgcc
     2581 ggatcgtggt tggcggcgac atagttgatc atcagccagt cgccggcctt gatcgtcacg
     2641 tcgccgaact gtgcgtcttc catcgccgtg cgcatgaagt gctggaccgg agtggtccag
     2701 cggatcgctt cctcgacgat ccccggcacc aggctgcggt cggccttgac gagggcgaac
     2761 tgatccgggt ccttggccag ggcgagcatg gccccggcgg tgctggcgct ggtggtgtcg
     2821 tgcccggcgg cggcgacgat gatgtagtat ccggcgcggt cgcgggcgga aatgtcggcg
     2881 ttggccagaa tcgtcgcgac gtcctcggtc gggttcttgc gccgctcggc cgccaggccc
     2941 tcgaaatagg tctcgaattc ggccacggcg ccgaacacga tctggctgat catttcgggc
     3001 gtcatctggg ccatgccgct tttcgacagg tcctcgtcct gcccgccgaa catctgctgg
     3061 gtcaggaaca gcatcttcgg ctcgtcctct tccggaacgc cgaggatctg catgacgacg
     3121 tggagcggat agggcgcagc gaccaagggc acgaaatcgg cctcgccgcc gttctcggcc
     3181 gccgccgcga tcagccggtc ggcggtcttg ccggccagct cacgcaccgc cgcctcgatc
     3241 gtcttgaggt tcttgggcat gaaccagtcc tgcgtcaggc ggcgcagctt gggatgcttg
     3301 ggcgcgtcga gcgcgaccag cgaggagacc aggttggggc tttcgtcgcc ctgcttggcg
     3361 gtgacgtcgc gggccagccg ctcgccctcc ttgttggtga acacggtcgg gcgcggggcg
     3421 ttgaggaagc gcacgttgtc cttcgaaacg cgcagcgcgt cctcgtgccg ggtcagcagc
     3481 cagaacgggt cgtgttcgcc ggtcgggctc tcgatccgca ccaccggcgt ttcggcgcgc
     3541 aggcggtcaa acaggtccag cgccgcgtcc cagtccgcat aggacttggg atcgatcacc
     3601 gccgcgccgt cttcgggcga agcgaccggt ccgatggccg cgctcatgcc gcgccccctt
     3661 gcgccttttc ataggcttcc ttgtactcgt cgcgcagcag ccgcttgtag agcttgccgg
     3721 tcagttcccg gggcagctgt tcgcggaaat cgatctggcg cggcatcttc acgcgagaga
     3781 gctgcggggc gaggaattcg gtcagctcct cggccagggc cgggccggca tcggccatgt
     3841 tgagcggctg gaccacggcc acgaccttct cgccaaagtc gggatcgggc gcgccgatca
     3901 cggcggcatc ggccaccttg gggtgggtga ccagcaggtt ctcgatctcc tgcgggtaga
     3961 tgttgacccc gcccgagatg atcatgaagc tcttgcggtc ggtcaggaac agatagccgt
     4021 cctcgtcctg gtagccgaca tcgcccaggc tggtccaacc gtgcttgttg cgggcatcgg
     4081 ccgtcttctg cgggtcgttg tggtattcga acggcaccat gccttcggtc ggttcgaaga
     4141 agatctgccc ttccgtgccg cgcggcactt catcgccgct ctcgtcgcag acgcggatga
     4201 tcccggtcag cgccttgccg accgtgccgg gcttctgcag ccattccggg ctggtgacga
     4261 aggtgaagcc gttgccctcg gtcccggcgt agtattcata gatcaccggg ccccaccatt
     4321 cgatcatcgc ctgcttgacc ggcaccgggc acggcgcggc ggcgtggatc gcgcacttca
     4381 gggtcgagac gtcgtacttc ttgcgcacgt cctcgggcag cttcagcatc cgcacgaagt
     4441 gggtcggcac gaactggctg tcggtgatct tgtacttctc gatcgcggcc agggcgtttt
     4501 cgggatcgaa cttttccatc accaccacgg tgccgcccag cttctggatg gtcatcgacc
     4561 agcgcagcgg cgcggcgtga tagagcgggg cgggggagag atagaccgaa tccggcttga
     4621 tcccgaaagc catcatggtg aggccgacca gggcattggc ctgatcgatc gccggatcct
     4681 cgggcagggg gatcttcacg cccttgggcc gcccggtcgt gccgctggaa tagagcatgt
     4741 ccacgccggc ccgttcgtcc gcgatcgggc tctcgggcat ggcggccagg gcgtcctcgg
     4801 ccgaaagcgc gccgggggtg tcaaagcgca gctgctccac ctgcggggcc agcttggcca
     4861 cctcgtccag ggtcggcgcg agataggccg aaccaaacag gatcttcgca ccgctgtctt
     4921 ccaggatata ggcaatctcg ggcggggtca ggcggctgga aatgcccaca tagtgcacgc
     4981 ccgaacgctg gaagccccag gtcaggcaga agaaccaggg gtggttttcc atgcacagcg
     5041 ccaccgtatc gccgacctgg atcccgcggc tacggagcag ctgggcgacc tggttggaac
     5101 ggcggtccag ctcggcaaag gtgatcgtct caccgcttcc ggccatgatg atggcgggac
     5161 gatcgggctg gttcttggca tggatcgacg gatgcatggg aatcactctc ctgttgcggt
     5221 cagcgccgct tggggggagt tttaaccgca cggttaaacg gcgcaagcgc tagttgcatt
     5281 gcccgcgcgg gcgcggtgcg gcgatcggcg caggcctggc acggcccggc gcagcggggc
     5341 gatcccgggt taggtattcg tatgcctatt gccccggctc cgccgccgtg caacccacgg
     5401 gggcaatgcc ataaggcaag ctaaagggag tgggaccgcg ccatggaaaa gccgcgtcaa
     5461 tcgtgggccg ggctggccaa tatcagtttc gggttcttcg gcatccagat cggcttcgca
     5521 ctgcagaacg ccaacatgag ccggattttc cagacccttg gcgccaacat ggacgatctg
     5581 gcgtggctgt gggcggccgc gccgctgacc gggctgctgg tccagccgat catcggccac
     5641 atgagcgaca agacctggct gggcaagctg ggacggcgcc ggccctattt tctggcgggt
     5701 gcggtgctgg ccgcgctctc gctgttcctg atgccgatca gcccgatgct gatgatggcg
     5761 gcgatgctgc tgtggatgct ggacgccagc ctgaacattt cgatggagcc gttccgcgcc
     5821 tttgtcggcg atatgctgcg caaggaccag cacaccgccg gctatgccgt gcagaccgcc
     5881 tttatcgggc tgggcgcagt cgtcggttcc ctgttcccgt ggctgctcga tcactggggc
     5941 gtcgccaacg aagccgcgcc cggggtgatc cccgataccg tccgctacag cttctgggcg
     6001 ggcggcgcgg cgctgtttgc cgcggtgatg tggaccgtgt tcagcacaag cgagtattcg
     6061 cccgagcaga tggccggctt tggcgaagcc gctgagtccg gcgccccctc gggcgcgaat
     6121 ctggctgggc gggacagcgg cgggcctttt gcctggatca tggccggggc ggcgctgatg
     6181 gtggtcactc accagttcgc gctggaaaag gaggtctacc tgctttcggc gctgctgatc
     6241 ggttacggcc tgatcagcca catcgcgatc cgcctggtcc gtgccggccg ttcggccggg
     6301 atgctgaccg cgatcgtcgg ggatttttcg gggatgcccg acatcatgaa gcggctggca
     6361 gtggtccagt tcttcagctg gtcggcgctt ttcacgatgt ggatctacac gaccgggatc
     6421 gtcgcccagc gctcgttcgg aacaaccgac aaggccagcg cggcctttca ggaggccggc
     6481 aactgggtcg gcgtgctgtt ctcggtctac aacggggtgg ccgccgtcgc cgcgctgacc
     6541 ctgctgccgt tcctggcccg gcggtttggc aaggcccgga cccacatgtt cggcctgctg
     6601 tgcggggcgg cgggctatgc cagcttcttc gtgatcaccg atccgcagtg gctgatcctg
     6661 agcgaagtgg gcgtcggcat tgcctgggcc tcgatcctgg ccatgcccta tgccattctg
     6721 gccagcagcc tgccgcaagc caagctgggc atctacatgg ggctgttcaa cgtcttcgtg
     6781 gtggtgccgc agctgctggt cgcaaccgtc atgggcacga tcatgaagca cttcttcccg
     6841 ggtgacccga tctggaccat ggcattcgcg gcgggcacgc tggtggtggc cgcgctcgcc
     6901 atgacccggg tcaaggtgcc cgacgaagcc tagaaatcgg cgaccagctg gagcgtgacg
     6961 gcccgcggcg tggtgtaggt gaacccgccc gaggacgaga cgttccagcc gtagttgtcg
     7021 aacacgttga gcaggaccgg gcgcaccagc caggtccccc cggccgcctt gaagcggtag
     7081 cgtgcgccca ggttcaccgc ggtgcgcggc ggggcgaaca gcgtgttggc ggcgttcccc
     7141 gcccggctgg agaggctttc caccgccagg tcgaacgaga gcgggccctt gccgtgcccg
     7201 ctgcgccagt cgagcgtcag caccgagcgc cgccgcacct ggccgaccgg ccggccgccg
     7261 atcaatccgc tgcgcaccgc ctcgcccgag atttccggat caagtagcaa ggtgccgccg
     7321 acgaccgtca gccccggcac gatctgcccg gtcagcgaga attcgaggcc gcggttgcgc
     7381 aggctgccaa gctggcgata gcgcagcgcc ggatcgaggt tgtagtaagg cttggtgatc
     7441 gagaaggccc cggcgatcag ggtcagccgc ggggtgacag cgtacttcag tccggcctcg
     7501 aactggcggg tgcggaccgc cggcggggcc tcggcccggt tgatcgccac gtcgggcgcg
     7561 gccagcgcct cctccagccc ggtcgaatag ccgccataga ccgccaggcc cggcgccagg
     7621 gtgaccgtgc cggtgacgtt ccagaccagc ggcccgtcgc gggcgatctg gtcggccagc
     7681 agcggatcgg cgaaatcggt ggtcttgcgg taggcggtct tggacaggct gacgtccagc
     7741 ccgaagcgcc cttcgcccac caccgaccag gacgcgccat aggtcagctg gcggatccgg
     7801 tcctcgttct tggggccaat cgtgtagacc ggttcgggcc gcacatcgcg ggtcaggatc
     7861 gtgctggggc ccagcgcgat cgactggctg ccgccaaagc ggcggtcgcg cgaacggccg
     7921 cgcaggctga aagtcagccg ctgattgatc ccgccggtct gccattgccg gaccagccgc
     7981 acttcgcccg aaagcgaggc atcgcggttg ttgccatcgg ccacgacccg ccgtgcgttc
     8041 accgatccgt cctggttcac gccgacatag agatcggaaa agatgctgcg ctggtcgcgc
     8101 ccgttgtaga acagcccggc ctccagccgg aacgggccga gcggggcctt gacgatcccg
     8161 ccataggccc agttcgccgc gttgcgcggg gtccagggct gggtcaggtc ggtcccgcgc
     8221 ggcgcttcgg gcggcggcgt ggtgccggac ggatagtaga tcggcttcgc ctcttcgctg
     8281 cggaacagga aggccccgcc gaacaccgcc acgctgatct ggtcggtcgg gcgccaggcc
     8341 agggtcgcgc caaaggtgcg gtacagcgca tccccgcctt cccagcgccg cgcgttgcgg
     8401 aagccgaacc cgccggaaat gcccagccgg ctgccgtcga tcggctgctt gaattcgaac
     8461 gatccgccgg ggccggcggc gttgttgccg gccagttcca gattgaagct gtaactggtc
     8521 gcgccgcgcg gctgggtcag cgaaaaatcg accagcccgg tcggggcggg gaagggatag
     8581 cgcagcccgg cggggccaac gcggatcgtg ctgccatcgc tcagccgggg ggacagccgg
     8641 tccacctggt cgtagtaaag cccttccagc cggacgttgc cggcatcgac cggatcgaac
     8701 ccgcgcacgt catcgctgga atagagcccg gaccggtccg agccgaccga gcggccgaag
     8761 gcgtcgctcg attgggtggc ggcgttctcg tccgcgcgct gggccaatgc cgcttgcggt
     8821 agcagcgcca gcacaagcac cgcctgcggc agcagcgcga gcacaagcgc caatccggcc
     8881 gaagcccccc atttcgattt gcaacgcatc gcgcgcccat agcgcgggcg cgcgttttgc
     8941 aaagcgcttt gttgggaccg ggcgtcaggc gtcgccgcgc tcggccgggt cctcgtgcag
     9001 ccaggcggcg tggcgcgggg cgcgctt
//
LOCUS       DASUDV010000289         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1069933,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000289 DASUDV010000000
VERSION     DASUDV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069933"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VFV30_06280"
     CDS             complement(<1..1829)
                     /locus_tag="VFV30_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4847731.1"
                     /translation="MLGSGKADSGQPARDRPASGQELQQALELLRDYESSGLGWFWAS
                     DGEGKITYVSDCIAALLGCSRSDILGQPVQSLFILERDEEDTIERPLPLILSAHKTFS
                     DLQVRAALGEKEVWWSISGRPQFDKDCNFVGYRGNGTDITASRQSQRDASRLAQYDSL
                     TGLANRHRMGKRLNATLTAYQSAKRCCAIMMMDLDRFKAVNDTLGHPAGDELLKQVAQ
                     RLQRVLNDKGFEIGRLGGDEFQIMLPDIDDRGRLGEIAKTIITMVSQPYQIEGSRCVI
                     GASVGIAIAPYDGINSEELVRSADLALYAAKGGGRGQFRFYSSDLHSEAERRRQIEED
                     LRDALSSDQMRVAYQPIIDTKTNKVVSLEAFARWEHPELGDVPPGVFIPIAEESNLIG
                     QLGDWVLKQACTEAAQWPGGVRIAVNVSAAQFGNAGFASVVAQALAHTGLPPERLELE
                     LTESIFLADHHDTNEMFKQLKLLGVRLALDDFGTGYSSLSYLQHAPFDKIKIDKSFIR
                     GVTQEGNRNAAIISSIVSLAKALDMETTAEGIEAHDELNAMRKLGVGQIQGFIYAAAV
                     SAEAVSEAMTTGEWVIEPEGPSKYRTERRTVLRKVGLIHEDHR"
     gene            complement(1981..>2260)
                     /locus_tag="VFV30_06285"
     CDS             complement(1981..>2260)
                     /locus_tag="VFV30_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4847732.1"
                     /translation="GGQHYHGTIRNISATGAMVEGLWNVPPGTIFTIIFAEGQMVTAT
                     ARWCQQDRMGVEFASPLEIDEAGRISLLAPQASDPRRSLLRRESGIAA"
BASE COUNT          366 a          743 c          742 g          409 t
ORIGIN      
        1 tagcggtgat cctcgtggat caggccgacc ttgcgcagca cggtgcggcg ttcggtgcgg
       61 tacttgctgg ggccttcggg ctcgatcacc cattccccgg tggtcatggc ctcgctgacc
      121 gcctcggcgc tgaccgcggc ggcatagatg aagccctgga tctgcccgac gcccagcttg
      181 cgcatggcgt tcagctcgtc gtgcgcctcg atcccttcgg ccgtggtctc catgtcgagc
      241 gccttggcca ggctgacgat cgaggaaatg atcgccgcgt tgcggttgcc ttcctgggtg
      301 acgccgcgga tgaaggactt gtcgatcttg atcttgtcga acggcgcgtg ctgcagatag
      361 ctgagcgagg aatagccggt cccgaaatcg tcgagcgcca gccgcacgcc cagcagcttc
      421 agctgcttga acatttcgtt ggtgtcgtga tggtcggcca ggaagatcga ctcggtcagt
      481 tccagttcca gccgctcggg cgggaggccg gtgtgggcca gcgcctgggc cacgaccgag
      541 gcaaagccgg cgttgccgaa ctgcgccgcc gaaacgttga ccgcgatccg caccccgccc
      601 ggccactgcg cggcttcggt gcaggcctgc ttcagcaccc aatcgcccag ctggccgatc
      661 aggttggact cttccgcgat cgggatgaag acgcccggcg gcacgtcgcc cagttcgggg
      721 tgctcccagc gggcaaaggc ttcgagcgaa acgaccttgt tggtcttggt gtcgatgatc
      781 ggctgatagg cgacccgcat ctgatcgctg gacagcgcat cgcgcaggtc ttcctcgatc
      841 tggcggcggc gctcggcctc ggaatgaagg tcgctggaat agaaccggaa ctgcccgcgc
      901 cccccgccct tggcggcata gagcgcgagg tcggccgaac ggaccagctc ttcggaattg
      961 atcccgtcat aaggcgcgat agcaatgccg accgaagccc cgatgacaca gcggctgccc
     1021 tcgatctgat agggctggct gaccatggtg atgatcgtct tggcaatctc gcccagccgc
     1081 ccgcggtcgt cgatatcggg cagcatgatc tggaattcgt cgccgccgag acggccgatc
     1141 tcgaagccct tgtcgttgag cacgcgctgc agccgctggg cgacctgctt gagcagctcg
     1201 tccccggccg ggtggcccag ggtgtcgttg accgccttga accggtccag gtccatcatc
     1261 atgatcgcgc agcagcgctt ggccgactgg taggcggtca gcgtggcatt gagccgcttg
     1321 cccatgcggt ggcggttggc gaggccggtc agcgaatcgt actgcgccag acgcgaggca
     1381 tcgcgctggc tctggcggct ggcggtaatg tcggtgccgt tgccgcgata gcccacgaag
     1441 ttgcagtcct tgtcgaactg cggccggccc gagatcgacc accacacttc cttttcgccc
     1501 agcgcggcgc gcacctgcag gtcggaaaag gtcttgtggg cggacaggat cagcggcagc
     1561 ggccgctcga tcgtgtcttc ctcgtcgcgt tccaggatga acagcgattg gaccggctgg
     1621 cccaggatgt ccgaacggct gcagcccagc agggcggcaa tgcagtccga gacataggtg
     1681 atcttgcctt cgccgtcgct ggcccagaac cagccaaggc ccgaggattc gtaatcgcgg
     1741 agaagttcca gcgcctgctg cagttcctgg ccgctggcgg gccggtcacg cgcgggctgc
     1801 ccggaatcgg ccttgccgct tcccagcatg gtgcgaaacc gcccgaaagg tgaaccccca
     1861 ctcatttcaa aaccgaccag tcacattccc cgcaagaccg gatggtccgg tcgcttgccc
     1921 gggaataagg ggatatggtt accatgcgcc taacggatgc gctgccggga cgcgccgcga
     1981 tcatgccgca atccccgatt cgcgccgcag cagcgaccgc cgcggatcgc ttgcctgggg
     2041 ggccagcaac gagatccggc cggcctcgtc aatttccagc ggactggcga attccacgcc
     2101 catccggtcc tgctggcacc accgcgccgt ggcggtaacc atctggcctt cggcgaagat
     2161 gatcgtgaag atcgtgcccg gcggcacgtt ccacagccct tcgaccatcg ccccggtggc
     2221 cgagatgttg cggatcgtgc cgtgatagtg ctgcccgccg
//
LOCUS       DASUDV010000290         6183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_782479,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000290 DASUDV010000000
VERSION     DASUDV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782479"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFV30_06290"
     CDS             complement(<1..617)
                     /locus_tag="VFV30_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX4847733.1"
                     /translation="MKRISIAERPNWTQIARDNGFTFHHVGGAKYWDESAYWQLSLGE
                     LEREIEDPTVELYAMCLALVDDVVRSEELMERMAIPAAMRDPIARSWFSGAPSLYGRF
                     DFAYDGSGPAKMLEFNADTPTSIYETAFFQWQWLEDTVAAGKLPAGADQFNRLHEALI
                     ERFARIFTPGSLVHFASVDDHVEDRQTVLYLEDLASQAGMEARFVA"
     gene            713..1363
                     /locus_tag="VFV30_06295"
     CDS             713..1363
                     /locus_tag="VFV30_06295"
                     /inference="COORDINATES: protein motif:HMM:NF018408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HEX4847734.1"
                     /translation="MTERATPFANRPKRSGRVGLSRMAGAVSGVALLAGCGSNEPAPV
                     QAPEEKGPKIEVAAFENQFECQANSGLGEEGCAEARKQAVATAAETAPRFAAAYDCEA
                     EWGQGNCMEQTQGGQSFFMPFVTGFLLGKVLTGNKRDIVPLFRKTKDGPLQTANGVRL
                     GYGGAPGKYVAAARALERPTAIPKIRSASPASSQAGMTQQQESSGRRGFAVVRTGG"
     gene            complement(1380..2513)
                     /locus_tag="VFV30_06300"
     CDS             complement(1380..2513)
                     /locus_tag="VFV30_06300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847735.1"
                     /translation="MQPTAAERPAVPAACAAADWADRQDPDVQRAWDDLALRASEPSP
                     FQERWYLQPALEQLDPQGTVRLVRVDSGGTLIGLIPVRAEPRYYRRPIPHLASWVHGN
                     CFLGLPLVAAGQEQPFWRAFLHWADRNAGRALFLHLSTLPLEGPLWQALQAVTAEQGR
                     LAGLVYREERALLASELSPADYFEASLSGKKRKELRRQMNRLAELGELHFERQRNAEG
                     LEQWNEEFLALEHSGWKGTAGSALASHHTSADMFRAAMQGAAVLGKLERLTLRLDGAP
                     IAMLINFLTPPGSFSFKTAFDERYSRFSPGVLIQRENLDLLADPAVEWCDSCASADHP
                     MIDHIWRERRAIGHVSIAIGGRLRRAAFGALLRRELGGSPTGL"
     gene            complement(2635..4728)
                     /locus_tag="VFV30_06305"
     CDS             complement(2635..4728)
                     /locus_tag="VFV30_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4847736.1"
                     /translation="MKSKTRFLRSAVAAALALTLAAPAVSQTTPGKPVATKVPLNVMA
                     ELPAMDDIAISPDGTKAAITLGSGNDFAYAIIDLVKGGKPKIYATAATYKEAGQRAVQ
                     GYRWVNDRYLLITLSSRENIFGQRIDLRRLAAYDTETGKVTPIAWRDTTGDGADVLYI
                     DKDKARILLARQVDTGNTETRFRYRVEWVDVATGQPIEIVQQTNPIVGSWFADNKGVV
                     RLATSSDRDSGESKLLYRSKAGEPITTVQKVKDTNFTGAGMQPLGFLDEPDMAVVSSN
                     HEGRRAIYKANMKTMTIVEKLFEAPEGYDAGRAIIDTDTNRILGYTYASDRPRQVWVD
                     PELKTIAEFLDEGFGEGNWYLLSRSRKNAKLVIGLAQPNQLASYYVYDTASGQMQLIG
                     YDNATIKDGLMNPVSTVRYKASDGLEIEAVVTTPRLRAGQKKLPVVVLVHGGPYGVRD
                     YAMYDQWAQSIAEQGYVVVQPNYRGSGGYGTEFVKAGRQDGFGTRMQDDLNDVVDFLA
                     AQGTVDPSRACMMGWSYGGYASARAAQRDAARWRCTIAGAGVYDLPMMRDYDQGYLGS
                     FGANYLAKGANSLVDVSPARNAGGKWSPILIVHGVRDPRVPIAQGRALASALRGAGKK
                     EGVEWAYLEQPKNGHYGVFFTKEERLEWLGGASAWLDRFNPAYVPSDPDYAKKPEADP
                     AVTAMGQRLFAPASK"
     gene            complement(4858..>6183)
                     /locus_tag="VFV30_06310"
     CDS             complement(4858..>6183)
                     /locus_tag="VFV30_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX4847737.1"
                     /translation="MALPLLIGGVCIVTSIIGTYFVRLGDGTNVMGAMYKGFLVSAVL
                     AVPAIWWATGYALGGMEAALPGQTFNGRELFYCSLLGLVITGLIIWITEYYTGTNYRP
                     VRSIAKASETGHGTNVIQGLAISLESTALPTLVIVAGIIIAHQLAGLIGIAYAATAML
                     ALAGMVVALDAYGPVTDNAGGIAEMAGLDDSVREKTDLLDAVGNTTKAVTKGYAIGSA
                     GLAALVLFAAYTEDLKYFSSALGLSGPVNFSLENPYVIVGLLLGALLPYLFGAMGMTA
                     VGRAAGDVVVDVREQFKNNPGIMTYETKPDYARTVDLVTKAAIKEMIVPSLLPVLAPI
                     VVYFVIAAVAGPSNGFAALGALLLGVIVGGLFVAISMTSGGGAWDNAKKYIEDGNHGG
                     KGSEAHKAAVTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAALAGGH"
BASE COUNT         1037 a         2006 c         2091 g         1049 t
ORIGIN      
        1 gccacaaacc gcgcttccat cccggcctgg ctggccagat cctccagata gagcacggtc
       61 tggcgatcct cgacatggtc gtcgaccgaa gcgaagtgga ccaggctgcc gggcgtgaag
      121 atccgggcga accgctcgat cagcgcctcg tgcaggcggt tgaactggtc agcgccggcc
      181 ggcagcttgc ccgccgcgac cgtgtcttcc agccattgcc actggaagaa cgcggtttcg
      241 tagatgctgg tcggggtgtc ggcgttgaat tccagcatct tggccgggcc gctgccgtca
      301 taggcaaagt cgaaccggcc atagagcgag ggcgcaccgg aaaaccacga ccgggcaatc
      361 ggatcgcgca tcgccgcggg aatggccatg cgctccatca gctcttccga gcggaccaca
      421 tcgtccacca gggccaggca catcgcgtaa agctcgaccg tggggtcctc gatctcgcgc
      481 tccagttcgc cgagggacaa ttgccaatag gcgctttcgt cccaatactt ggcgccgccg
      541 acgtggtgaa aggtaaagcc gttgtcgcgc gcgatctgcg tccagttggg ccgctctgca
      601 attgaaatgc gcttcatgat cggcactatc cccgtcagaa tttactaacc atatggtaat
      661 tcggccttgt tatggcaatc acgataccga actgatccac atggatgctg ccatgaccga
      721 acgtgcgacc ccttttgcaa accggcccaa gcgctctggc cgtgtcggcc tgtcccggat
      781 ggcgggggcc gtcagcgggg ttgccctgct ggccgggtgc ggcagcaacg agcccgcgcc
      841 ggtccaggcg cccgaggaaa agggcccgaa gatcgaggtt gccgcgttcg agaaccagtt
      901 cgaatgccag gccaattcgg gtctgggcga agaaggctgc gccgaagcgc gcaagcaggc
      961 cgttgccacc gccgccgaaa ccgcaccgcg ctttgccgcg gcctatgatt gcgaagccga
     1021 atggggccag ggcaactgca tggagcagac ccagggcggg cagagctttt tcatgccctt
     1081 cgtcaccggc ttcctgctgg gcaaggtgct gaccggcaac aagcgcgata tcgtgccgct
     1141 gttccgcaag accaaggacg gtccgctgca gaccgccaac ggcgtgcggc tgggctatgg
     1201 cggcgctccg ggcaagtatg tcgccgcggc gcgcgcgctg gaacggccga cggccattcc
     1261 caagatccgc tcggcttcgc cggcctcgtc gcaggcgggc atgacccagc agcaggaatc
     1321 ctccggccgc cgcggctttg ccgtagtccg caccggcggc tgatccgccg ctttccggtt
     1381 tacaggcccg tcgggctgcc gcccagttcg cggcgcagca aggcgccgaa ggccgcgcgg
     1441 cgcaggcgcc cgccaatcgc aatcgaaacg tgcccgatcg cgcggcgttc gcgccagatg
     1501 tgatcgatca tcgggtgatc ggccgacgcg cagctgtcgc accattccac cgctggatcc
     1561 gccagcaggt cgaggttttc gcgctggatc aggacgcccg gcgaaaagcg cgaatagcgc
     1621 tcgtcaaagg cggtcttgaa cgagaacgag cccggcgggg tcaggaagtt gatcagcatg
     1681 gcgatcggtg caccgtcgag ccgcagggtg agccgttcca gcttgcccag cacggccgcg
     1741 ccctgcatcg cggcgcggaa catgtccgcg ctggtgtggt gcgaggcgag ggccgaaccg
     1801 gcggtcccct tccagccgga atgctccagc gcgaggaact cttcgttcca ttgctccagc
     1861 ccctccgcgt tgcgctgccg ttcaaagtgc agctcgccca gctcggcgag gcggttcatc
     1921 tggcggcgca gttccttgcg cttcttgccg gacaggctgg cttcgaaata gtccgccggg
     1981 ctgagctctg aagccagcag cgcgcgctct tcgcggtaga ccagcccggc cagccggccc
     2041 tgttcggcgg tcaccgcctg aagggcctgc cacagcggcc cctcgagcgg cagggtggag
     2101 aggtggagga acagcgcgcg cccggcgttg cggtccgccc agtgcaggaa ggcgcgccag
     2161 aacggctgct cctgaccggc ggcgaccagc ggcaggccaa gaaagcagtt gccgtggacc
     2221 cacgacgcga ggtgcgggat cgggcggcgg taatagcgcg gctccgcgcg gaccgggatc
     2281 agcccgatca gcgtcccgcc gctgtcaacc cggaccagtc ggactgtgcc ctgcgggtcc
     2341 agctgttcca gcgcgggctg caggtaccag cgttcctgaa agggggaggg ctcggacgcg
     2401 cggagggcca gatcgtccca ggcacgctgg acatcggggt cttgccggtc tgcccagtcc
     2461 gccgccgcgc aggcggccgg aaccgccggc cgctcggccg cggttggctg cacgcgcaaa
     2521 tcggttcgtg tttcagccgc ggtggccaaa ccctggatcc ctggcaccgg cggccggatt
     2581 gccgccgggc agggatttag cattgagttg ccaaggaaag gctaacgtgg agccttactt
     2641 ggacgccgga gcgaacaggc gctggcccat ggccgtcacc gccggatcgg cttccggctt
     2701 cttggcatag tccggatcgc tggggacgta ggccgggttg aaccggtcca gccaggccga
     2761 ggcgccgccc agccattcca gccgctcttc cttggtgaag aagacgccgt agtggccgtt
     2821 cttgggctgc tccagatagg cccattccac gccttccttc ttgcccgcgc cgcgcagcgc
     2881 cgaggcaagg gcacggccct gggcgatggg tacgcgcgga tcgcgcacgc cgtggacgat
     2941 caggatcggc gaccacttgc ccccggcgtt gcgggccggc gagacatcga ccagcgaatt
     3001 ggcgcccttg gcaaggtagt tcgcgccgaa cgagccgagg taaccctggt cgtaatcgcg
     3061 catcatcggc aggtcataga ccccggcgcc ggcaatggtg cagcgccagc gggccgcatc
     3121 gcgctgggcc gcgcgggccg aggcgtagcc gccataggac cagcccatca tgcaggcgcg
     3181 gctcggatcg acggtgccct gcgcggcgag gaaatcgacc acgtcgttga gatcgtcctg
     3241 catccgggtg ccaaagccgt cctggcgccc ggccttgacg aactcggtgc catagccgcc
     3301 cgagccgcgg tagttcggct ggaccaccac atagccctgt tcggcaatcg actgggccca
     3361 ctggtcgtac atcgcatagt cgcgcacgcc gtagggaccg ccgtggacca gcaccacgac
     3421 gggcagcttc ttctgcccgg cgcgcaggcg cggggtggtg acgacggcct cgatttccag
     3481 cccgtcgctg gccttgtagc ggaccgtcga gaccgggttc atcagcccgt ccttgatggt
     3541 cgcgttgtca tagccgatca gctgcatctg gccgctggcg gtatcgtaga cgtaatagga
     3601 cgccagctgg ttgggctgcg ccagcccgat gaccagcttg gcgttcttgc ggctgcgcga
     3661 gagcaggtac cagttgccct caccgaagcc ttcatccagg aattcggcaa tggtcttgag
     3721 ctccggatcg acccagacct ggcggggacg gtcgctggca taggtgtagc cgaggatccg
     3781 gttggtgtcg gtgtcgatga tcgcgcggcc ggcgtcatag ccttccggcg cttcgaacag
     3841 cttttcgacg atggtcatcg tcttcatgtt ggccttgtag atcgcccggc ggccttcgtg
     3901 gttgctggaa acgacggcca tgtcgggctc gtccagaaag cccagcggct gcatcccggc
     3961 gccggtgaaa ttggtgtcct tgaccttctg caccgtggtg atcggttcac ccgccttcga
     4021 gcggtagagc agcttgcttt cgccgctgtc gcggtccgac gaggtcgcca ggcggacgac
     4081 acccttgttg tcggcaaacc agctgccgac gatcgggttg gtctgctgga caatctcgat
     4141 cggctggccg gtggcgacat cgacccattc gacgcggtag cggaaccggg tttcggtatt
     4201 gccggtatcg acctggcggg cgagcaggat gcgggccttg tccttgtcga tgtagagcac
     4261 gtccgcaccg tcgccggtcg tatcacgcca ggcgatcggg gtaaccttgc cggtttcggt
     4321 gtcataggcc gccaggcggc gcaggtcgat ccgctgaccg aagatgttct cgcggctgga
     4381 cagggtgatc agcaggtagc ggtcgttgac ccaacggtag ccctggaccg cgcgctggcc
     4441 ggcttccttg taggtggccg ccgtggcata gatcttgggc ttgccgccct tgaccagatc
     4501 gatgatcgca taggcgaaat cgttaccgct gcccagcgtg atcgcggcct tggtgccatc
     4561 gggcgagatg gcgatatcgt ccatcgccgg cagttcggcc atgacgttga gcgggacctt
     4621 ggttgccacc ggcttgccgg gggtggtttg cgaaaccgcc ggggcggcaa gggtcagggc
     4681 cagggcagcg gcaaccgcgc tgcgcagaaa acgggtcttg gatttcacgc gacgtaatcc
     4741 ttcttccagc cgcgagtgcg gcatcgatga aggaagtgta gcagcttgct cccaaaggaa
     4801 aaccccgccc ggatcgctcc ggacggggtt tttccttcac ctttgtacag gtggggctca
     4861 gtgcccgccc gccagcgcgg cgagcaggag cagcgccacg atgttggtga tcttgatcat
     4921 cgggttcacg gccggaccgg cggtgtcctt gtagggatcg cccacggtgt cacccgtgac
     4981 ggcggccttg tgggcttcgc tgcccttgcc gccgtggttg ccgtcttcga tgtacttctt
     5041 ggcgttgtcc cacgcgccgc cacccgaggt catcgagatc gcgacgaaca ggccgccgac
     5101 gatcacgccg agcagcaggg cgccgagcgc ggcaaagccg ttcgacggac ccgcaacggc
     5161 ggcgatcacg aagtagacca cgatcggggc cagcaccggc agcagcgacg gaacgatcat
     5221 ttccttgatc gccgccttgg tcaccaggtc cacggtccga gcatagtccg gcttggtttc
     5281 gtaggtcatg atgccggggt tgttcttgaa ctgctcgcgc acgtccacca ccacgtcgcc
     5341 ggcggcgcgg cccacggcgg tcatgcccat cgcaccgaac aggtacggga gcagcgcgcc
     5401 gagcagcagg ccaacgatga cataggggtt ttcaaggctg aagttcaccg gaccggacag
     5461 gcccagcgcc gaggagaagt acttcaggtc ctcggtatag gcggcgaaca ggaccagcgc
     5521 ggcaaggccg gccgaaccga tcgcataacc cttggtgacg gccttggtgg tgttgccgac
     5581 ggcgtcgagc aggtcggtct tttcacggac cgaatcgtcg agacccgcca tttcggcaat
     5641 gccgccggcg ttgtcggtca ccggaccata ggcgtcgagc gcgacgacca tcccggccag
     5701 cgccagcatg gcggtggcgg cgtaggcaat cccgatcagg cccgccagct ggtgggcgat
     5761 gatgatgccc gccacgatga ccagggtcgg cagcgcggtc gattccaggc tgatcgccag
     5821 gccctggatc acgttggtgc cgtggcccgt ttccgaagcc ttggcgatcg agcgcaccgg
     5881 acggtaattc gtaccggtgt agtactcggt gatccagatg atcaggccgg tgatgaccag
     5941 gcccagcagc gagcagtaga acagctcgcg tccgttgaag gtctgacccg gcagggcggc
     6001 ttccatgccg cccagggcat agccggtggc ccaccagatc gccggaacgg cgagcacggc
     6061 cgaaaccagg aagcccttgt acatcgcacc catcacgttc gtgccgtcac ccaggcggac
     6121 gaagtaggtg ccgatgatgc tggtaacgat gcagacgccg ccgatcagca gcggcagcgc
     6181 cat
//
LOCUS       DASUDV010000291         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_693861,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000291 DASUDV010000000
VERSION     DASUDV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693861"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1443)
                     /locus_tag="VFV30_06315"
     CDS             complement(58..1443)
                     /locus_tag="VFV30_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEX4847738.1"
                     /translation="MIRLFKHYIPHAVVLLSLIDLLLLLLAGDLSWRLRAAQIGMDPG
                     AVVERIWPLGGLTAVVFTAMIAVGVYGADALRSMRFATARILVAISLAIIALAFIDFV
                     LGGGNFWRSTLAYAMFGSVILLVLNRLVVGGILGAAAFRRRVLVMGAGHRADRLRKLS
                     ERPESGFSIVGYLAMTDNQPVVREAISRGAIENLTRFVEDRGASEVVLALEERRNALP
                     LADLLRIKTTGVHVNDFSSFIERETGRVDLDTVNPSWLIFSDGFSSGRAISSAAKRIF
                     DILASSLLLVLSGPLILLFALLVKVDSRGPAFFRQRRVGLYGQHFDVIKLRSMRIDAE
                     ANGAQFAQKDDPRVTRLGRFIRKVRIDELPQTWSVLKGEMSFVGPRPERPEFVCELEQ
                     QLPYYAERHMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYSPFLDLLILLQT
                     VRVVLWPEGAR"
     gene            complement(1528..2919)
                     /locus_tag="VFV30_06320"
     CDS             complement(1528..2919)
                     /locus_tag="VFV30_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX4847739.1"
                     /translation="MSETAILGGPVIRRLRRKESLTQAAMAARLGISPSYLNLIERNQ
                     RPLSARLLVQLAEQFDFDPRSLRQADVVGGIDGLRRRLADDRFSDLSIDRDEIAEWLS
                     AAPQTALAFARLFDTAGQGGAAAPSDPMEQVRREIERWRNHFADLDVAAEELSDELRL
                     SNAEIGAALVERLRQRHQLSVRILPFDVMPDALRRLDLHARQLQLSELLDQSSRNFHI
                     AVQLALLEQKDAITALADGARFADRAAWRLFRRHLAGYFAAAVLMPYGRFMRACEATG
                     YDLAILQRRFTVGFEQLAHRLTTLQRVGQRGLPFFMARVDRAGQFSKRYAGASGTLLL
                     ESDRSCPLWVVHQAFERAGRICRQLVEFHDAASQPSQWLTLARTVDGSGTLGGGNAQF
                     AVILGLEAGLAGQLAQSGAMEIAPERAIPIGPGCARCHRADCQQRSLPPLGMTLEFDE
                     RSRGLTPFDFNRR"
     gene            3070..>3792
                     /locus_tag="VFV30_06325"
     CDS             3070..>3792
                     /locus_tag="VFV30_06325"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX4847740.1"
                     /translation="MTYSDSISKAGEIISQNSNWDGINAESVARMRLQNRFKTGLDIA
                     RYTAGIMRADMAAYDKDPAAYTQSLGCWHGFIGQQKLISIKKHFGTTKGRYLYLSGWM
                     VAALRSDFGPLPDQSMHEKTSVAALIEELYTFLRQADARELGMLFRELDEARKTGDEV
                     KAKATQAKIDNYETHVVPIVADIDAGFGNAEATYLLAKKMIEAGACCIQIENQVSDEK
                     QCGHQDGKVTVPHEDFIAKIRAV"
BASE COUNT          783 a         1232 c         1159 g          618 t
ORIGIN      
        1 ccggcaaacc aggccagctg gccaacgaag ctccaggcaa gaccggccac gcccatctca
       61 gcgcgcccct tcgggccaga gcacgaccct tacggtctgc aacaggatca gcagatcgag
      121 gaacgggctg taattcttgg cgtaatagag atcgtattcc agcttgtgcc gggaatcctc
      181 gatcgaggca ccataggggt aattgatctg cgcccacccg gttatgccgg gcttcaccat
      241 gtggcgttcg gcataatagg gaagctgctg ttccagctcg caaacgaatt ccgggcgctc
      301 gggacgcggg ccgacaaagc tcatttcgcc cttcagcacg ctccaggtct gcggcagttc
      361 gtcgatccgg actttgcgga tgaagcggcc caggcgggtc acgcgcggat cgtccttctg
      421 cgcgaactgc gcgccgttcg cttcggcatc aatccgcatc gatcgcagct tgatcacgtc
      481 gaagtgctgg ccatagaggc ccacgcgccg ctgcctgaag aacgccggcc cgcggctgtc
      541 gaccttcacc agcagggcaa acagcaggat cagcgggcca gaaagcacca gcagcagcga
      601 actggccagg atatcgaaaa tccgcttcgc cgcgctcgag atcgctcggc cggacgagaa
      661 accatcggaa aagatcagcc agctgggatt gaccgtatcg agatcgaccc ggccggtctc
      721 acgttcgatg aagctggaaa agtcgttgac atggaccccg gtcgtcttga tccgcaacag
      781 atcggccagc ggcaaggcgt tgcgccgttc ttccagcgcc agcaccacct cgctcgcgcc
      841 gcgatcctcg acaaagcggg tcaggttttc gatcgcgccg cgactgatcg cctcgcgcac
      901 cacgggctgg ttgtcggtca tcgccaggta gccgacgatc gaaaagccgc tttcgggccg
      961 ttcggaaagc ttgcgcaggc gatcggcgcg gtggcccgcc cccatcacca gcacccgccg
     1021 ccgaaacgcc gccgctccca gaatgccgcc cacgaccagg cggttcagca ccagtagaat
     1081 aaccgaaccg aacatcgcgt aggccagtgt cgagcgccag aagttgccgc cgcccagcac
     1141 gaaatcgatg aatgccagcg cgatgatcgc cagcgatatc gccaccagga tacgggccgt
     1201 ggcgaagcgc atcgagcgca gcgcatcggc gccatagacc ccgacggcaa tcatcgcggt
     1261 aaagaccacc gccgtcagac cgcccagggg ccagatccgc tctaccaccg cgccgggatc
     1321 cattccgatc tgcgcggcgc gcagccgcca cgacaggtcg cccgccaaca gcaacagcaa
     1381 gaggtcgatc aggctgagca ggacgaccgc atgcggaatg tagtgtttga acaggcggat
     1441 catgccgctg cctggccttc cctcaaagtc tcgagggttc ggcctagcgc cgagaagtga
     1501 aatgtcggta aattttacag gctttggtta acgccggttg aagtcgaacg gagtgaggcc
     1561 acggctgcgt tcgtcgaatt ccagggtcat gcccagcggc gggagcgatc gctgctggca
     1621 gtccgcacga tggcaccggg cacagccagg accaatcgga atcgcccgtt cgggcgcgat
     1681 ctccatggcg ccagactggg cgagctggcc tgcaagccct gcctcaaggc ccaggatcac
     1741 cgcgaactgg gcattccctc cgcccagcgt gccgctgcca tcgacggttc gggccagggt
     1801 cagccattga ctgggctggc tggcggcatc gtggaattcg accagctggc ggcaaatccg
     1861 gcctgcccgt tcgaatgcct ggtgcaccac ccacaagggg caagagcgat cgctttccag
     1921 cagcagggtg ccgcttgccc cggcatagcg cttggaaaac tgtccggcgc ggtcgacccg
     1981 ggccatgaag aagggcaagc cgcgctgccc gacccgttgc agcgtggtca gccggtgggc
     2041 gagctgttcg aaccccaccg tgaagcgccg ctgcaggatt gccagatcat agccggtcgc
     2101 ctcgcaggcc cgcatgaaac ggccataggg catcagcacc gcagccgcga aatagccggc
     2161 cagatgacgc cgaaacagcc gccaggcggc ccgatcggca aagcgcgcgc catcggccag
     2221 ggcggtgatc gcatccttct gctcaagcag ggcaagctgc accgcgatgt gaaagttgcg
     2281 cgacgactga tcgagcagtt cagacaactg caactgccgg gcgtgaagat cgagccggcg
     2341 caaggcatcg ggcatcacat cgaagggcag gatccggacc gatagctgat ggcgctggcg
     2401 caagcgctcg accagggcag cgccgatttc ggcgtttgac aggcgcagtt cgtcggaaag
     2461 ctcctcggcg gcgacatcca ggtcggcaaa atggttgcgc cagcgttcga tctcgcggcg
     2521 cacctgctcc atcggatcac tcggcgccgc cgcgccgcct tgcccggcgg tatcgaacag
     2581 ccgggcaaag gccaaggcag tctgcggcgc ggcgctcagc cattcggcga tctcgtcgcg
     2641 gtcaatcgac agatcggaaa agcgatcatc ggcaaggcgc cgccgcaagc cgtcgatccc
     2701 gcccaccaca tcggcctggc gcaggctgcg cggatcgaaa tcgaactgtt cggcaagctg
     2761 gaccagcagg cgtgccgaaa gcggacgctg gttgcgttcg atcaggttga gatagctggg
     2821 cgaaatgcca aggcgtgcgg ccatggcagc ctgggtcagg ctttccttac ggcgcagccg
     2881 gcggatgacc ggcccaccca ggatggcggt ttcgctcatg agatccgtgt atgtaaatta
     2941 ttttacatct ttgcaaacaa tttacgccgt tcacgcgcat acgtacaata ttttctgtaa
     3001 tttttattac cgtccggcgg gtcaagcgag ttagtgattg gtcacttcct cccgcctgga
     3061 gatacttccg tgacctattc cgattcgatc agcaaagccg gcgaaatcat cagccagaac
     3121 agcaactggg atggcatcaa tgccgaatcc gttgcccgca tgcggttgca gaaccgcttc
     3181 aagaccgggc tcgacatcgc ccgctacacc gccgggatca tgcgcgccga catggccgcc
     3241 tatgacaagg atccggccgc ctacacccag tcgctgggtt gctggcacgg cttcatcggc
     3301 cagcagaagc tgatctccat caagaagcat ttcggcacca ccaagggccg ctacctctac
     3361 ctctcgggct ggatggttgc cgcgctgcgc agcgactttg gcccgcttcc ggaccagtcg
     3421 atgcacgaga agacctcggt cgccgcgctg atcgaggagc tctacacctt cctgcgccag
     3481 gctgatgccc gcgaactggg catgctgttc cgcgagctcg atgaagcccg caagaccggc
     3541 gatgaagtca aggccaaggc aacccaggcc aagatcgaca actacgaaac ccatgtggtt
     3601 ccgatcgtcg ccgatatcga tgccggcttc ggcaacgccg aggcgaccta tctcctcgcc
     3661 aagaagatga tcgaggccgg ggcctgctgc atccagatcg aaaaccaggt ttcggatgaa
     3721 aagcagtgcg gccaccagga cggcaaggtt accgtgccgc acgaagactt catcgccaag
     3781 atccgcgccg tg
//
LOCUS       DASUDV010000292         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1820907,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000292 DASUDV010000000
VERSION     DASUDV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820907"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /gene="metX"
                     /locus_tag="VFV30_06330"
     CDS             <1..401
                     /gene="metX"
                     /locus_tag="VFV30_06330"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834121.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX4847741.1"
                     /translation="GRKLQDRDQKTFGFDADFQVESYLRYQGLSFTDRFDAHSYLYIT
                     RAMDYFDLAEEHGGRLADAFAKSTARFCLVSFDTDWLYPTSESRTVAHALNASGARVS
                     FVELSAPFGHDSFLLDVPALDRVVEGFLSA"
     gene            398..988
                     /gene="metW"
                     /locus_tag="VFV30_06335"
     CDS             398..988
                     /gene="metW"
                     /locus_tag="VFV30_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX4847742.1"
                     /translation="MSALRSDLAIIAANVAKGARVLDVGCGDGALMAALRDESGVDAR
                     GMELDAQNVAACVARGLSVIQGDADADLANYPDAAFDYAILSQTLQTTKRPDRVLDEL
                     LRVGRKGFVSFPNFAHWRVRFSLLWNGKMPVTRLIPVAWYATENIHHLTVADFRALVS
                     ERGYKVEGAWFLANGKPIGPMAANWRAEHAVFLLSR"
     gene            complement(980..1756)
                     /gene="fdhD"
                     /locus_tag="VFV30_06340"
     CDS             complement(980..1756)
                     /gene="fdhD"
                     /locus_tag="VFV30_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX4847743.1"
                     /translation="MSQRLIEIGADGTHHAANREWVPEAPVALEYNGLAYAVMMATPQ
                     QLDDFATGFALSEGLATTASDVGPCDIAQVEDGWVVRTMLSGLGIDKLTERVRARVAE
                     SSCGLCGIENLAEVARPLPPVAAHQAIEPGAIFSALAALRGHQDLGNRTGAAHAAAWA
                     NTQGQIGMVREDVGRHNAMDKLAGAMARSDQALSPGFVLSTARCSYEIVEKAVRNGAT
                     TLVTVSLPTSLAVERARQAGLSLWVLARGDSVLLANDASA"
     gene            complement(1753..2130)
                     /locus_tag="VFV30_06345"
     CDS             complement(1753..2130)
                     /locus_tag="VFV30_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4847744.1"
                     /translation="MYSHNMVGSNDIARSKTFYDALFTAVGGKPGIQDDKGRLIYMHN
                     GGLFLVTQPIDGAPATAGNGCTIGFAMESPAQADAWHAAGVAHGGTAIEDPPGVREGG
                     FGNLYLAYLRDPDGNKLCALHRM"
     gene            2247..2798
                     /locus_tag="VFV30_06350"
     CDS             2247..2798
                     /locus_tag="VFV30_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4847745.1"
                     /translation="MAQINPGSGPARPCRINQILHRAAAIFQRDGKPRELIVPTAFAN
                     LSLLPADESLRQLPMQLARLGQRRRLANMANFLRSDFNRIVLDCPAGLSEVSDQVIAA
                     ADLLIVPLPASPLSARALDMLRRELSGNHHRHPPILPVLSMFDASRAMKRLWTGIENK
                     LAEMNAAERKLHASFSPMKAPAP"
     gene            2894..>3159
                     /locus_tag="VFV30_06355"
     CDS             2894..>3159
                     /locus_tag="VFV30_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847746.1"
                     /translation="MLALSAFPVQARETGPGELARELDNPRPQKAMGDMLGSMMGAML
                     DIKADPLARAMERMGEGEAARRIPKGATIGELAGPEARQVPAEVR"
BASE COUNT          524 a         1012 c         1036 g          587 t
ORIGIN      
        1 tcggccgcaa gctgcaggac cgtgaccaga agaccttcgg gttcgatgcc gatttccagg
       61 tcgaaagcta tctgcgctat caggggctga gctttaccga ccggttcgat gcacactcct
      121 acctttatat cacccgggcg atggactatt tcgacctggc ggaagagcat ggcggacggc
      181 tcgccgatgc tttcgccaaa tcgaccgcgc gcttctgtct ggtcagtttc gataccgact
      241 ggctctatcc aacctcggaa agccgcacgg ttgcccatgc gctcaacgcc tcgggcgcgc
      301 gggtcagctt tgtcgagctg tccgcgccct tcggacatga cagcttcctg ctggatgtgc
      361 cggcgcttga ccgggttgtc gaagggttcc tttcggcatg agcgcgctgc gatccgatct
      421 ggcgatcatc gccgccaatg tcgccaaggg tgcgcgggtg ctcgatgtcg ggtgcggcga
      481 tggcgcgctg atggctgcgc tgcgcgatga gagcggggtc gatgcccgcg gcatggagct
      541 tgatgcgcaa aacgtcgccg cctgcgtggc gcgcgggctt tcggtgatcc agggtgatgc
      601 cgatgccgat ctggccaact atcccgacgc cgccttcgat tatgcgatcc tcagccagac
      661 gctgcagacc accaagcggc ccgaccgggt gcttgacgaa ctgctgcggg tcgggcgcaa
      721 gggcttcgtc agctttccca atttcgcgca ctggcgggtg cggttctcgc tgctgtggaa
      781 cggcaagatg ccggtcaccc ggctgatccc ggtggcctgg tacgcgaccg aaaacatcca
      841 ccatctgacc gtggccgatt tcagggcgct ggtaagcgaa cgcggctaca aggtcgaagg
      901 ggcgtggttc cttgccaatg gcaaaccgat cgggccaatg gccgccaact ggcgggccga
      961 acacgcggtg ttcctgctgt cacgctgagg cgtcgttcgc cagcaagacc gaatcgccgc
     1021 gcgccaggac ccacaggctg agcccggcct gccgtgcgcg ctcgaccgca agcgaggtgg
     1081 gcagcgacac cgtaaccagc gtagtggctc cgttgcgcac cgccttctcg acgatttcat
     1141 aggaacagcg cgccgtgctg aggacaaagc cggggctcag cgcctggtcc gagcgggcca
     1201 tggcgccagc cagcttgtcc atcgcgttgt gccggccaac atcttcgcga accataccga
     1261 tttgcccctg ggtgttggcc caggctgcgg cgtgggcagc gccggtgcga ttaccgagat
     1321 cctggtgacc gcgaagcgcc gcaagcgccg agaaaatcgc gcccggttcg atcgcctggt
     1381 gcgccgcgac tgggggcagg gggcgggcca cttcggcaag gttttcgatc ccgcacagcc
     1441 cgcagctcga ttcggccacg cgtgcccgca cgcgctcggt cagcttgtcg ataccaaggc
     1501 cggacagcat ggtgcggacc acccagccgt cttcaacctg agcgatgtcg catgggccga
     1561 cgtccgaggc cgtcgtggcc aggccttcgc tcaaggcaaa gccggttgcg aagtcatcga
     1621 gctgctgcgg tgtggccatc atcaccgcat aggcaaggcc attgtattca agcgccaccg
     1681 gggcttcggg cacccattcg cggttggccg cgtggtgggt gccgtccgcc ccgatttcga
     1741 tcaggcgctg gctcacatcc ggtggagcgc gcagagcttg ttgccgtccg gatcgcgcag
     1801 ataggccagg taaaggttgc cgaagccgcc ttcacgcacg cccggcggat cttcgatcgc
     1861 ggtgccgcca tgggccacgc ctgcggcatg ccaggcgtcc gcctgggccg ggctttccat
     1921 ggcaaagccg atcgtgcagc cattgccggc cgtggccggc gcgccatcga tcggctgggt
     1981 caccaggaac aggccgccat tgtgcatgta gatcagccga cccttgtcgt cctggatccc
     2041 cggcttgccg cccaccgcgg tgaagagggc atcgtagaag gtcttcgagc gggcaatgtc
     2101 attgctgccc accatgttgt gactgtacat ggaccggttc ctttcagttg cgaaagaaaa
     2161 ctgcataagc gattcacgcg gggcgtcaat tgccacgtgc ggccaggacc gattgggtcg
     2221 ctgcgggggc agcttattct tccgatatgg cacaaatcaa tccggggtcg ggtcccgcta
     2281 gaccttgtag aatcaatcaa atcttgcatc gtgccgcggc catctttcag cgcgatggca
     2341 agccgcgcga actgattgtg ccaaccgcct ttgccaatct ctctttgctg ccggcagacg
     2401 agagtttgcg tcagttgccg atgcagctgg cccggcttgg ccagcgccgc cgcctggcca
     2461 acatggccaa tttcctgcgc agcgatttca accgcatcgt gctcgattgt cccgccgggc
     2521 tcagcgaggt gtccgaccag gtgatcgccg cagccgacct gctgatcgtg cccttgccgg
     2581 cttcgccgct ttcggcccgg gcgctcgata tgctgcgccg cgagttgagc ggcaaccatc
     2641 atcgccatcc gccgatcctg ccggtcctat cgatgttcga cgccagcagg gcgatgaagc
     2701 ggctttggac cgggatcgaa aacaagcttg cggaaatgaa cgcggctgag cgcaagctgc
     2761 atgcttcgtt cagccccatg aaagcgccgg cgccctagcg gcgcgccaaa tcccggtgcc
     2821 ccgggatgcg gagattggcg ccatgactat tcgttctggt tcgatcaggt tgcttggggg
     2881 tgccgctggc gctgtgctgg cgctgtctgc gtttccagtc caggcccgtg aaaccgggcc
     2941 tggtgaactg gcccgcgaac tcgacaatcc caggccgcaa aaggccatgg gcgacatgct
     3001 gggttcgatg atgggcgcca tgctcgacat caaggccgat ccgctggcgc gggcgatgga
     3061 gcggatgggc gagggggaag ccgcgcgccg gatccccaag ggtgcgacga tcggcgaact
     3121 ggccggtccc gaggctcgcc aggttcccgc cgaagtgcg
//
LOCUS       DASUDV010000293         3939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1599772,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000293 DASUDV010000000
VERSION     DASUDV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599772"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFV30_06360"
     CDS             complement(<1..509)
                     /locus_tag="VFV30_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX4847747.1"
                     /translation="MDRAVFPPETLAGLAAIYPGTPGKLRHNLLVHPLLTIDALADLA
                     ARLPGDSIEYNPSELPIGIAPEAVPQADLGVVETIRSIAKAGSWVALKRIEQDQAYGQ
                     LLRDILAELRGVVEPRTGAMMQLEGFIFISSPGSVTPFHFDPEHNILLQIMGSKTMTL
                     FPVEDEDLFA"
     gene            650..2578
                     /locus_tag="VFV30_06365"
     CDS             650..2578
                     /locus_tag="VFV30_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX4847748.1"
                     /translation="MDADGKKAATVFLSYARADQKRALPVIKALEQAGIEVWWDGLIE
                     GGDSFLPTTEAALEDADAVVVLWSKTSVDSHWVRDEATRGRDRGCLVPLSIDGTHPPL
                     GFRQFQVIDFAKWRGRQDATELRRALRAIHGFAGHAAPHPAPAAAARGRIDRRMVIGG
                     GAALLAGGAALAVWKGGLIGGADSPGNSVAVLPFRNLSGDPAQDFFAEGIAEQIRTTL
                     SRNEKLLVLAPASIASVAKDGVEDLAAIAEKLGVAFLLGGSVRRSGDQLRIAATLTDA
                     KSGFSSWNEQFEKRLEDVFAVQDEIADAVAAVMAAQTAGGGKSNSRELGGTSSVRAYD
                     AYLRGNAFYNLRSGEAAYRSALAQYDAALERDADFALALAARARVIVVLTNNYAQASQ
                     FKAAYDDALASARKAVQLAPQLASAQSTLGYVLVQAKLDLKGAWKHHQEARRLGSGDA
                     GVLNLYSAYMAQMGRKREAAEGIERAIKLDPLNPGVFRIAAFVAYCARDYPRVIEHAR
                     KGIALNPKMESIHAYLGDALLQLGKPEEARAEYLREGGGLRGDTGLAIVEHKLGNAAA
                     AQAAMDALIKAFGDATTYQQAQILAQWGEADKAMAKLAFAREIGDVGLAQAYTDPLLD
                     PLRGRPDFSRLLTELGFG"
     gene            2610..2843
                     /locus_tag="VFV30_06370"
     CDS             2610..2843
                     /locus_tag="VFV30_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847749.1"
                     /translation="MKKMLLAGLAGSIMLLAACGQKAEEAAPAETAAATDAAAADASA
                     APAADASAAPAADASAAPSAASTDAGTSGGVEP"
     gene            complement(2918..>3939)
                     /locus_tag="VFV30_06375"
     CDS             complement(2918..>3939)
                     /locus_tag="VFV30_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX4847750.1"
                     /translation="GPDPKAPSGYLARKWFGAADPGAARALANRRPAPQLRLVSSISG
                     GFGLSRGASGVYLVKDGAAPYQAQINYATNPAKWGGEFAEFGRFDDWEARHICGGTLI
                     APEWVLTAAHCFVPKPTSNAQRKLNKGINVLLGAENLARPQSGMTYRVDRVIIHGGFR
                     PGFIYRHDIALVHIVANRSTSRLNQISTIQRYAGTEPADKTGVSVTGWGKTRELKTEL
                     AATELWRADVKLIASGRCRALPSFGKIDPKKSGPANEKLSAGSLCAGESRGKACSGDS
                     GGPLVFTNGAPPRLLGVTSWTVSESCGKPQIPNVYTRVAAYEQWIRDAMAAPAKPWTV
                     TRFGK"
BASE COUNT          655 a         1253 c         1357 g          674 t
ORIGIN      
        1 gcgaacaggt cttcgtcctc caccggaaac agggtcatcg tcttcgaacc catgatctgc
       61 agcaggatgt tgtgttccgg atcgaagtgg aacggggtga ccgatccagg cgaggagatg
      121 aagatgaagc cttcgagctg catcatcgct ccggtccgcg gctcaaccac cccgcgcagt
      181 tcggccagga tatcgcgcag cagctggcca taggcctgat cctgctcgat ccgcttgagc
      241 gccacccacg atcccgcctt ggcgattgaa cggatcgtct cgaccacgcc cagatcggcc
      301 tggggcaccg cttcgggggc aatcccgatc ggcagctcgc tggggttgta ttcgatgctg
      361 tcgccgggca gccgcgcggc cagatcggcc agggcatcga tggtgagcag gggatggaca
      421 agcaggttgt gacgcagctt gcccggcgtt ccaggataga tcgcggccag gccggcaagc
      481 gtttcgggcg gaaaaacagc gcggtccata agggtctcct ggaccgcgcg ctagcatcag
      541 gaagttaaga tcgggttggg caggctcgcg attgacgaac gcgcggcgga ctggcacttc
      601 gcagggcgag aacaagggtt gccgcacaag gcacggggga tccaccgcaa tggacgcgga
      661 tggcaagaag gcagcaaccg ttttcctgag ctatgcccgg gccgaccaga agcgcgccct
      721 gccggtgatc aaggcgctgg aacaggccgg catcgaggtg tggtgggacg gcctgatcga
      781 aggcggcgac agcttcctgc caaccaccga ggcggcactg gaagacgccg atgcggtggt
      841 ggtgctgtgg tcaaagacct cggtggattc gcactgggtg cgcgacgagg cgacccgcgg
      901 gcgcgatcgt ggctgcctgg tgccgctgtc gatcgatggc acccatcccc cgctgggttt
      961 tcgccagttc caggtgatcg actttgcgaa gtggcgcggc cgccaggatg cgaccgagct
     1021 gcggcgtgcg ctccgggcga tccatggctt tgccggacac gctgcgcccc atcccgcccc
     1081 ggcagctgcc gcgcgggggc ggatcgaccg gcgcatggtg atcggtggcg gcgccgcgct
     1141 gctggccggc ggggcggcgc tggcggtgtg gaagggcggg ctgatcggcg gcgcggacag
     1201 ccccggcaac agcgtggcgg tgctgccgtt ccgcaacctt tcgggcgatc cggcgcagga
     1261 tttcttcgcc gaggggatcg ccgaacagat ccgcaccacg ctttcgcgca acgaaaagct
     1321 gctggtgctg gctccggcct cgatcgcatc ggtggctaag gacggggtgg aagacctcgc
     1381 cgcgatcgcc gaaaagctgg gggttgcctt cctgctgggc ggttcggtgc gccgttcggg
     1441 cgaccagctg cggatcgccg ccaccctgac cgatgccaag agcggctttt ccagctggaa
     1501 cgaacagttc gaaaagcggc tggaagacgt cttcgcggtg caggacgaga ttgccgatgc
     1561 ggtggccgcg gtcatggccg cgcagaccgc gggcggcggc aagtccaaca gccgcgagct
     1621 gggcggcaca tcgagcgtgc gggcctacga tgcctatctg cgcggcaacg ccttctacaa
     1681 cctgcgctcg ggcgaggcgg cttatcgttc ggcgctggcc cagtacgatg ccgcgctgga
     1741 gcgcgatgcc gatttcgccc tggcgctggc agcgcgggcc cgggtgatcg tggtgctgac
     1801 caacaattat gcccaggcca gccagttcaa ggccgcctac gacgatgccc tggcatcggc
     1861 ccgcaaggcg gtgcagctgg cgccgcagct ggccagcgcg caatcaacgc ttggttatgt
     1921 cctagtccag gccaagcttg atctgaaagg tgcctggaag caccaccagg aggcccgccg
     1981 gctgggatcg ggcgatgccg gggtgctcaa cctctacagc gcctacatgg cgcagatggg
     2041 ccgcaagcgc gaggcggccg aagggatcga acgggcgatc aagctcgatc cgctcaatcc
     2101 aggcgtgttc cggatcgcgg ctttcgtggc ctattgcgcg cgcgattatc ccagggtgat
     2161 cgaacacgcc cgcaagggca tcgcgctcaa ccccaagatg gaatcgatcc atgcctatct
     2221 tggcgatgcc ctgctgcagc tgggcaagcc cgaagaagct cgggccgaat acctgcgcga
     2281 aggcggcggg ctgcgcggcg atacgggtct ggccattgtc gaacacaagc tgggcaatgc
     2341 cgctgcggca caggcggcga tggacgcgct gatcaaggcc tttggcgatg ccacgaccta
     2401 tcagcaggcg cagatcctgg cgcagtgggg cgaggccgac aaggccatgg ccaagctcgc
     2461 ttttgcccgc gagatcggcg acgttggcct ggcccaggcc tacaccgatc cgctgctcga
     2521 tccgctgcgc ggcaggcccg atttttcccg gttgctaacc gagctgggct tcggatagtc
     2581 tcatcacggt taaccaggag gggaatgcca tgaaaaagat gctgcttgcc gggcttgccg
     2641 gttcgatcat gctgctggcc gcttgtggtc agaaggccga agaagccgct ccggctgaaa
     2701 cggctgccgc caccgatgct gccgctgccg atgcttcggc tgcgcccgcc gccgatgcct
     2761 cggccgctcc ggctgccgat gcttctgccg ctcccagcgc ggcttcgacc gatgccggaa
     2821 ccagcggcgg ggtcgaaccc tgatccaatc cgccggcacg ggctgaacaa ttcggggcgt
     2881 ggtcaccagg caggtggcca cgccctgatt gtttgagcta cttgccgaac cgggtcacgg
     2941 tccagggctt ggccggtgcg gccatcgcat ccctgatcca ttgttcgtag gccgccaccc
     3001 gggtatagac gttggggatt tgcggcttgc cgcagctttc gctgaccgtc caggaggtga
     3061 ctcccaacag ccttggtggc gcgccattgg tgaacaccag cggtccgccg ctgtcaccgc
     3121 tgcaggcctt gccgcggctt tcgcccgcac acagcgatcc cgcgctgagc ttttcgttgg
     3181 cgggaccgct tttcttcgga tcgatcttgc cgaaactggg cagcgcccgg caccggcccg
     3241 aggcaatcag cttgacatcg gcccgccaca attcggttgc cgccagctcg gtcttcagtt
     3301 ccctggtctt gccccagccg gttaccgaaa cgccggtttt gtcggcaggt tcggtgccgg
     3361 catagcgctg gatcgtcgaa atctggttga gccgcgatgt cgatcggttg gcaacgatgt
     3421 gcacaagggc aatgtcgtgg cgatagatga agcccgggcg gaagccgcca tggataatca
     3481 cccgatcgac ccggtaggtc atcccgctct ggggccgggc caggttttcg gcccccagca
     3541 acacgttgat ccccttgttc agcttgcgct gggcgttgga ggtgggcttg ggcacgaaac
     3601 agtgcgccgc ggtcagcacc cattcgggcg cgatcagcgt gccgccgcag atgtggcgag
     3661 cttcccagtc gtcgaacctg ccgaactcgg cgaattcgcc gccccatttg gccgggttgg
     3721 tcgcatagtt gatctgcgcc tggtagggtg cggcgccatc cttgaccaga tagacccccg
     3781 aggcgccccg gctgagcccg aacccgccgg aaatgctgct gaccagccgc aactgcgggg
     3841 ccgggcggcg gtttgccaat gcccgggcag cgccaggatc ggccgcgccg aaccatttac
     3901 gggcgagata gcctgatggc gccttgggat ccgggccgg
//
LOCUS       DASUDV010000294        10356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_672117,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000294 DASUDV010000000
VERSION     DASUDV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10356
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672117"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..2814)
                     /locus_tag="VFV30_06380"
     CDS             complement(43..2814)
                     /locus_tag="VFV30_06380"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX4847751.1"
                     /translation="MGSRAEDHRVTAPRIANQRAVIDRRALAGAITDLVEAQGAAKAR
                     PKIVELLRGALAAGREEIARRLLERPSAGHLSAEAQAFLVDQLVRLIHDHVTTDVYPS
                     GNRSSGERLTIMAVGGYGRGEMAPHSDVDIAFITPIKQTPWCEQVIEAMLYFLWDLGL
                     KVGQSSRSLDEMVRMGRGDLTIRTAMLEGRYVWGDQALFDEAQSRFWAEVVAGTERQF
                     VTEKLAERNERHKRLGDSRYVVEPNVKEGKGGLRDLHTLYWIGKYIHHVRGASELVRV
                     GLLTPAEYRAFARAEDFFWAVRCHLHTITKRAEDRLTFDLQREVAARMQFADRPGKSA
                     VERFMQYFFLQAKRVGHLTGLFLAQLDDQFARKQPRGLLAGFRARRRMVKGYPVFGGR
                     INVPSEDWFTQDPVRLIEIFVLADAQGLELHPDAMRLAVRDARAVDAVRDDPRANALF
                     MDLLSSRNDPEVALRWFNEAGVFGRFMPDFGRVNAQMQFDMYHHYTVDEHTIRAIGLM
                     ARIEKGEMKQDHPLASAIVHKVVNRRALYVSILLHDIAKGRRGDHSVLGAEVALKLCP
                     RLGMSETETQLVSWLVRNHLLMSAVAFKRDLADWKTITDFVTQVQAIERLRLLAMLTI
                     VDIRAVGPGVWNGWKRQLIHELYEAAEERLRLGHVQHGREQRIAAKQRQVAERLGTSG
                     KLVDKYARQFNDAYWIAEPEDVIAKNLAQLDAAKGKALDVQTEYYAARGATLVTVIAA
                     DHPGLFYRIAGGIHLAGGNIIDARIHTARNGMAVDNFLVQDPLGRPFSEDSQLVRIET
                     MIADALGGRIKLVPQLAARPLARPRADAFDVRPSVEFDNTASNRFTVIEVNARDRPAL
                     LNRLARALFEAKLMVHSAHIATYGERAVDTFYVTDLLGEKVDAGARLKAVEKKLLEAA
                     DEDVIEVAA"
     gene            complement(2834..3529)
                     /locus_tag="VFV30_06385"
     CDS             complement(2834..3529)
                     /locus_tag="VFV30_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847752.1"
                     /translation="MIAEQITRNAQAIGRPVAKPGCQPNVWIAFLSDSKAQVAQLRKL
                     DPAMFDTLQSYEIERIFSGSGAAQVWHSTEIRNADGRPIQIMEFETTAGRKVEVGFTS
                     QYSAGRLVSPIRNDINATIVVFDRDRANGKTVRQLADYATMRIFAPVRDFDAVPEDVV
                     PTILLLFTPGADAPGGLTEFDWAYLAAYYKLDRGAQPSAVHDATRRTMLDGTGQKLSE
                     KAGQDGQGLTSPP"
     gene            complement(3980..5692)
                     /locus_tag="VFV30_06390"
     CDS             complement(3980..5692)
                     /locus_tag="VFV30_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX4847753.1"
                     /translation="MPTELDTALNSVVDRLTAPGGMLETTTFERFGRQLPMLKNAPPT
                     LPVMFEMFCAQHGDAEFLVDGEIRMTFAQTYAAARAVAGGLVAGHGVETGDRIGIAAR
                     NSTNWIVAYMAVLMAGGCATLLNGWWSGDEMAGGIELAGCKLVLADAQRAARIADLGL
                     ATKVVVFEHDCAWDKGLAALLANGGGAETALPALTPDDLATILYTSGSTGLSKGAYSD
                     HRGVTQGVFSYVAQTLMILTYLTERNEAPSVQPCSLVNVPLFHVTGEVPLFLQSFVIG
                     RKLVLMPKWNAEDAMRLIEQEKVTYFVGVPLMSFEIATHPNRDKYDLSSCTSFAAGGA
                     PRPVDHVKRIKDALPHAFPLLGYGLTETNAVGCGNLNENYLAKPGSTGTASKPLVELG
                     ILDDAGAELAAGQVGEVSIRSICNFLGYWNNTEATKDAITPDGWFRTGDLGYLDADGY
                     LFIVDRKKDIIIRGGENISCIEVEQAIYAHPDVAEASVFGMPDEKFGEQPTGVYLVKE
                     GRSLSEDDLKAYLLTLIAPFKVPVRLWQEHETLPRLGTEKVDKRTLKARYLTVWEAEQ
                     SASA"
     gene            5852..6760
                     /gene="lgt"
                     /locus_tag="VFV30_06395"
     CDS             5852..6760
                     /gene="lgt"
                     /locus_tag="VFV30_06395"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4847754.1"
                     /translation="MLSLSLATLAAAAGSAPIHFDDLGLSRSLFSLDTGWGTFELRWY
                     SLAYLAGILLAYWHTTKAAKLPGAPLAQRHIDDLFFYCTLGVILGGRLGYATFYTGGD
                     TGLPSLWAHPGELLQLWHGGMSFHGGLIGVALAIIWVCWRNKLAMLRVGDYIAVAVPF
                     GMLFGRLANFVNGELWGRVAGPDVPWAIVFPGAGEAARHPSQLYQAGLEGLLLAVILV
                     PLFWKTRARFRPGLLMGIFLFGMGLGRFVAEYFREPDEQLQEFARQTGLTMGQWLTIP
                     LILIGLFFVVRALRRPELGSGTPAPQ"
     gene            6757..7815
                     /locus_tag="VFV30_06400"
     CDS             6757..7815
                     /locus_tag="VFV30_06400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4847755.1"
                     /translation="MTDEAPAESLATIFRRLIANTGPISLMHYMGESNARYYGAKDPL
                     GAAGDFVTAPEISQMFGELIGLWLADMWINAGREEPVHYVELGPGRGTLAQDALRVAR
                     RYGLEPQVHFVETSTALKDVQLAGLPGAQWHDDLSTVPPYGPILLVANEFLDALPVRQ
                     LVMTPDGWRERMVVPEGDRFVCVAGSQPMDAAVPEARRQAEVGTILETCPGAAATVYE
                     VAGRLVEQGGAALFIDYGHDAPRDGSTLQAVRDHLKVDPFVGPGEADLTAHVDFATLA
                     PIALSRGCKWLGTVPQGRWLRNLGIDARAQALAERAPQHAAAIETAKERLVGEGQMGV
                     LFKVMGLCSPAWPGGAGF"
     gene            7924..8550
                     /locus_tag="VFV30_06405"
     CDS             7924..8550
                     /locus_tag="VFV30_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4847756.1"
                     /translation="MDKPHVRRPTLLSRVAKQVLLWFYHGKGWRLFGGLPETRKFVIA
                     GAPHTSNWDFVFFTGATHEMGIQPNFMGKHTLFKWPLKRFMEDMGGIPVDRTKRANYV
                     EQIAAEFARRDELALVIAAEGTRSTDGSWKSGFYHIAQAAGVPIVPTWVCNATKRLGF
                     GPALMPSGDYPADLAKIAAYLRSVLPDEPRFKVLEAQAMRLLKGEQDV"
     gene            8543..9343
                     /locus_tag="VFV30_06410"
     CDS             8543..9343
                     /locus_tag="VFV30_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX4847757.1"
                     /translation="MFEALLINAAILIATVLVLWAISVRINDVSFIDSFWGAGMGLMA
                     LISWMQVEPLPGALGTLIMAMTCAWGFRLGIYLLRRWRREGEDPRYVRILRKDREAGR
                     FAFAALTKVWLGQAFLLFLVSSPAQVAILASGAPAPVPPLAWAGLALYLVGIFFEWVG
                     DWQLARFKANKANEGRILDTGLWRYTRHPNYFGDCLAWWGIWLCCAGAGWPYALATLP
                     GPLFLTFTLTKWSGAALLEAGMKKKRGDKYADYIARTSAFVPWPPKQL"
     gene            complement(9347..>10356)
                     /locus_tag="VFV30_06415"
     CDS             complement(9347..>10356)
                     /locus_tag="VFV30_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX4847758.1"
                     /translation="ADAIVVLIHQGGQQGEGGDPNSCTGFTGEIRPILDRLDPRVDLV
                     VSGHTHRAYICDYSAYNPAKPFLLTSAEKNGMMVTDIALDIDPVAGRVVARRARQVVA
                     QSLPYASPRGGQVANTALFPLFTPRADVAAYVQRYTDAAKAFSSRPAGKLAGPASGWA
                     LGMLIADGQLEATRIAGAQIAFMNPFGVRAPLNPGEGGLLTFGDLYAAQPFNNTLITQ
                     SLTGAELKAVLEQSFDGNGPEQPLIPSAGFTYSYDKSRPVGQRIVAMRLNGQPIDPNG
                     MYRVTTNSFLAQGGDAFTVFAGQRTAQVGISDLDALEAWLKGDAPRAVPSETRAVAVG
                     N"
BASE COUNT         1573 a         3487 c         3500 g         1796 t
ORIGIN      
        1 gtggtgccaa ggacagggcg gatgccctgt ccgcaccgca aactaagccg cgacctcgat
       61 cacgtcctcg tcggccgcct ccaacagctt cttctccacc gccttcagcc gtgcgccggc
      121 gtcgaccttt tcgcccagca ggtcggtgac atagaaggtg tctaccgcgc gttcaccgta
      181 agtggcgatg tgggcggaat ggaccatcag cttggcttcg aacagcgccc gggcgagccg
      241 gttgagcagg gccggccggt cgcgcgcgtt cacttcgatc acggtgaagc ggttgctggc
      301 ggtgttgtcg aactccacgc tggggcgcac atcgaaggca tcggcgcgcg gccgggccag
      361 cgggcgcgcg gccagctgcg ggaccagctt gatccgaccg cccagcgcat cggcgatcat
      421 cgtttcgatc cggaccagct ggctgtcttc cgaaaacggc cgaccgagcg ggtcctggac
      481 caggaaattg tcgaccgcca tgccgttgcg cgcggtgtgg atccgcgcgt cgatgatgtt
      541 cccgccggcc aggtggatgc cgcccgcgat gcggtagaac aggcccgggt ggtcggccgc
      601 gatcacggtg accagcgtgg ccccgcgcgc ggcgtagtat tcggtctgga cgtcgagcgc
      661 cttgcccttg gccgcgtcga gctgggccag gttcttggcg atgacatcct ccggttcggc
      721 gatccagtag gcgtcgttga actggcgggc gtacttgtcg accagcttgc cggaggtccc
      781 cagccgctcg gccacctggc gctgcttggc cgcgatccgc tgttcgcgcc cgtgctggac
      841 atggcccagc cgcagccgtt cctcggccgc ttcgtaaagc tcgtggatca gctggcgctt
      901 ccacccgttc cacacgcccg gcccgaccgc acggatatcg acgatggtca gcatcgccag
      961 cagccgcagc cgctcgatcg cctggacctg ggtgacgaaa tcggtgatgg tcttccagtc
     1021 ggcgaggtcg cgcttgaagg cgaccgcgct catcagcagg tggttgcgca ccagccagga
     1081 caccagctgg gtctcggtct cgctcatccc cagccgcggg cagagcttca gcgccacctc
     1141 cgcacccagc accgaatggt cgccgcggcg gcccttggcg atgtcgtgca gcaggatcga
     1201 gacatagaga gcgcggcggt tgaccacctt gtggacgatc gcgctggcca gcggatggtc
     1261 ctgcttcatc tcgcccttct cgatccgcgc catcaggccg atcgcgcgga tggtgtgctc
     1321 gtcgaccgtg tagtggtggt acatgtcgaa ctgcatctgc gcgttgaccc gcccgaaatc
     1381 gggcatgaag cggccgaaca ccccggcttc gttgaaccat cgcagcgcca cttcgggatc
     1441 gttccggctg gacagcaggt ccatgaacag cgcgttggcg cgcggatcgt cgcgcaccgc
     1501 atccaccgca cgggcatccc gcaccgccag gcgcatggca tcggggtgca gttccagccc
     1561 ctgggcatcg gccaacacga aaatctcgat caggcggacc ggatcctggg tgaaccagtc
     1621 ctccgagggc acgttgatcc gcccgccgaa caccggatag cccttcacca tccgccgcct
     1681 ggcacgaaag ccggccagca gcccacgcgg ctgcttcctg gcgaactggt catccagctg
     1741 ggccaggaac agcccggtca ggtggccgac ccgcttcgcc tggaggaaga agtactgcat
     1801 gaaccgctcg accgcgctct tgcccgggcg gtcggcaaac tgcatccgcg ccgcaacctc
     1861 gcgctgcagg tcgaaggtca gccggtcctc ggcccgcttg gtaatggtgt gaaggtggca
     1921 gcgcaccgcc cagaagaagt cctcggcgcg ggcgaaggcg cggtattcgg ccggggtcag
     1981 caggccaacc cggaccagtt ccgacgcccc ccggacatgg tggatgtact tgccgatcca
     2041 gtagagcgtg tgcagatcgc gcaggccgcc cttgccctcc ttgacgttgg gttcaaccac
     2101 atagcggctg tcgcccagcc gcttgtgccg ctcgttgcgc tcggccagct tctcggtgac
     2161 gaactggcgc tcggtcccgg ccaccacttc ggcccagaac cggctctgcg cctcgtcgaa
     2221 cagggcctga tcgccccaga catagcgccc ttccagcatc gccgtgcgga tcgtaaggtc
     2281 gccgcgcccc atccgcacca tctcgtccag cgagcggctc gactggccca ccttgagccc
     2341 caggtcccac aggaaataga gcatcgcctc gatcacctgc tcgcaccagg gggtctgctt
     2401 gatcggggtg atgaaggcga tgtcgacgtc cgaatggggc gccatctcgc cccggccata
     2461 gccgccgacc gccatgatcg tcagccgttc gcccgatgag cggttgccgg agggatagac
     2521 atcggtggtc acgtggtcgt ggatcaggcg gaccagctgg tcgaccagga aggcctgggc
     2581 ctcggcgctg aggtgcccgg ccgaggggcg ctccagcagc cgccgggcaa tctcctcgcg
     2641 accggccgcc agcgcgccgc gcagcagctc gacgatcttc gggcgggcct tggccgcgcc
     2701 ctgcgcctcg accagatcgg tgatcgcgcc cgccagcgca cggcgatcga tcaccgcgcg
     2761 ctggttggcg atccgcggcg cggtcacccg gtggtcttct gctcggcttc ccataccgtc
     2821 aggtaacggg ccttcagggc gggcttgtca agccttgccc gtcttgcccg gccttttcgc
     2881 tcagcttctg gccggtcccg tccagcattg tccgccgggt tgcgtcatgc accgcgctgg
     2941 gctgcgcgcc gcgatcgagc ttgtaatagg cggccagata ggcccaatcg aactcggtca
     3001 ggccgcctgg cgcatcggcg ccgggggtga acagcaggag gatggtgggc accacgtcct
     3061 ccggcacagc atcaaaatcg cgcaccggcg cgaaaatccg catggtcgca tagtcggcca
     3121 gttgccgcac ggtcttgccg ttggcgcggt cccggtcgaa caccacgatc gtggcattga
     3181 tgtcgttgcg gatcggcgaa accagccgcc ctgcggaata ctgcgaagtg aaaccgacct
     3241 cgaccttccg gccagcggtt gtttcaaatt ccatgatctg gatcgggcgg ccgtcggcgt
     3301 tgcggatctc ggtggagtgc cagacttgtg cggcgccgct gccagagaag atccgctcga
     3361 tctcatagga ctgcagggta tcgaacatcg ccgggtcgag cttgcgaagc tgcgcaacct
     3421 gcgccttgct gtcgctgaga aatgcgatcc agacattggg ctggcacccc ggctttgcga
     3481 ccggccggcc gatcgcctgg gcgttccggg tgatctgttc cgcaatcaaa gcgccgtagg
     3541 ccgggtcaat tccgaacaac ttgacgcaga tcggggcgta tcgccggggc agcgggctac
     3601 cgatcggtgg ccgcaaggtc accaatttcc cctgttgcgc ggccgcctcg gccttttcga
     3661 cccggtcgcc gaccacgacg atcgagccgg aatcgccggc cccgctatgc gcaatgctgg
     3721 cggtcgctga caaaaaggct gccgccgcca gaagcgggcc aaatcgcctt cgcgcaattg
     3781 cagaaaccgg agaatatcgc atcttgcaag cagtccttga gctctgcggc cggccactcc
     3841 aggagcaatg gcacgcgtcc gtctggtcaa caaaacccgt tgcaaaggca ggaaaataat
     3901 aggctgaagc gaaaatgcgt caagccgaaa cacccgttcg gtgttccggc ttgcgcatta
     3961 cttggatcgt ccggtttgtt taggcggaag cgctctgctc ggcttcccat accgtcaggt
     4021 agcgggcctt cagggtgcgc ttgtcaacct tttcggtgcc gaggcgcggc agagtttcgt
     4081 gttcctgcca cagccgcacg ggcaccttga acggcgcgat cagcgtcagc aggtaggcct
     4141 tgaggtcgtc ctcggacagg ctgcggcctt ccttgacgag atagaccccg gtcggctgct
     4201 cgccgaactt ctcgtccggc atgccaaaga cgctggcctc ggccacgtcg gggtgggcat
     4261 agatcgcctg ctccacctcg atgcagctga tgttctcgcc gccgcggatg atgatgtcct
     4321 tcttgcggtc gacaatgaac agatagccgt cggcatcgag gtagccgaga tcgccggtgc
     4381 ggaaccagcc gtccggggtg atcgcgtcct tggtcgcctc ggtgttgttc caatagccga
     4441 ggaagttgca gatcgagcgg atcgaaacct cgcccacctg gccggcggcc agttcggccc
     4501 cggcatcgtc aaggatgccc agctccacca gcggcttgct ggcggtgccg gtcgatcccg
     4561 gcttggccag gtaattctcg ttgaggttgc cgcagccgac cgcgttggtt tcggtgaggc
     4621 catagcccag cagcgggaag gcgtgcggca gcgcgtcctt gatccgcttg acgtggtcca
     4681 ccggccgcgg cgcgccgccg gcggcgaacg aggtgcagct ggagaggtcg tacttgtccc
     4741 ggttcgggtg ggtcgcgatc tcgaagctca tcagcggcac gccgacgaaa taggtgacct
     4801 tctcctgctc gatcagccgc atcgcgtctt cggcattcca cttgggcatc agcaccagct
     4861 tgcgcccgat cacgaagctc tgcaggaaca gcggcacttc gccggtgacg tggaacagcg
     4921 gcacgttgac caggctgcac ggctggaccg agggggcttc gttccgttcg gtcaggtagg
     4981 tcaggatcat cagcgtctgg gcgacatagc tgaacacccc ctgggtcacc ccgcggtggt
     5041 ccgaataggc ccccttggag agcccggtgg agcccgaggt gtagaggatc gtggcgagat
     5101 cgtccggcgt cagcgccggc agggcggtct ccgccccgcc gccgtttgcc agcagcgcgg
     5161 ccaggccctt gtcccaagcg caatcgtgtt cgaacaccac caccttggtg gccagcccca
     5221 gatcggcaat gcgggcggcg cgctgtgcat cggccagcac cagcttgcac ccggcgagtt
     5281 cgatcccgcc ggccatttca tcgccgctcc accagccgtt gagcagggtg gcgcagccgc
     5341 cggccatcag cacggccatg taggcgacga tccagttggt cgaattgcgc gcggcgatcc
     5401 cgatccggtc gccggtctca acgccgtgcc ccgcgaccag gcccccggcc acggcgcggg
     5461 ccgcggcata ggtctgggca aaggtcatgc ggatttcgcc atcgaccagg aattcggcat
     5521 cgccgtgctg ggcgcagaac atctcgaaca tcaccggcag ggtcggcggc gcgttcttga
     5581 gcatcggcag ttgccggccg aagcgttcga aagtggtggt ttccagcatg ccgccggggg
     5641 cggtcagacg gtccacgaca gagttgaggg cagtatccag ttcggtgggc atcggcgtcc
     5701 ttctttcccg tttgacccgc ccgcgcccct ctcacgccgg cggacgccgg ggtttgtttc
     5761 ccggcttgct ctgtgccaca agcagcggcg ctcgcagagg aatctccgtg cgcgccgcgc
     5821 ggcggcgtcg atcatctaag gggttacgga cttgctgtca ctatcgctcg ccacgctggc
     5881 cgctgctgcc ggttccgcgc cgatccactt cgacgacctg ggcctgtcgc gcagcctgtt
     5941 ctcgctcgac accggctggg gcacgttcga gctgcgctgg tacagcctgg cctatctggc
     6001 ggggatcctg ctggcctact ggcacaccac caaggcggcc aagctgcccg gcgcgccgct
     6061 ggcccagcgc cacatcgacg acctgttctt ctattgcacg ctgggcgtga tcctgggcgg
     6121 gcggctgggt tatgccacct tctacaccgg cggcgacacc ggcctgccca gcctgtgggc
     6181 ccatccgggt gaactgctgc agctgtggca cggcggcatg agctttcacg gcgggctgat
     6241 cggggtggcc ctggcgatca tctgggtctg ctggcggaac aagctggcga tgctgcgggt
     6301 gggcgattac atcgccgtcg cggtgccgtt cgggatgctg ttcgggcggc tggccaactt
     6361 cgtcaacggc gagctgtggg gccgggtggc cgggccggac gtgccctggg cgatcgtctt
     6421 tcccggtgcc ggtgaggccg cccgccatcc cagccagctc tatcaggccg ggctggaagg
     6481 gctgctgctg gccgtgatcc tggtgccgct gttctggaag acccgcgccc ggttccgccc
     6541 gggtctgctg atggggatct tcctgttcgg catggggctg ggacggttcg tggccgaata
     6601 cttccgcgag ccggacgagc agctgcagga gtttgcccgc cagaccgggc tgaccatggg
     6661 ccagtggctg accattccgc tgatcctgat cggcctgttc ttcgtggtcc gcgcgctgcg
     6721 ccggcccgaa ctcggcagcg gaaccccggc tccgcaatga ccgacgaggc cccggccgaa
     6781 tcgctggcca cgatctttcg ccgcctgatc gccaacaccg ggccgatcag cctgatgcat
     6841 tacatgggcg aatccaacgc ccgctattac ggcgcgaagg acccgctggg cgcggccggc
     6901 gatttcgtca ccgcgccgga aatcagccag atgttcggcg aactgatcgg gctgtggctg
     6961 gccgacatgt ggatcaacgc ggggcgcgag gagccggtcc actatgtcga gctggggccc
     7021 ggccgcggca ccctggcgca ggacgcgctg cgggtggcgc ggcgttacgg gctggagccg
     7081 caggtccact tcgtcgagac ctcgaccgcg ctgaaggatg tgcagctggc cggactgccc
     7141 ggcgcgcagt ggcacgacga cctgtcgacc gtgccgccct atggcccgat cctgctggtc
     7201 gcgaacgagt tcctcgatgc cttgccggtg cgccagctgg tcatgacccc cgatggctgg
     7261 cgcgagcgga tggtggtgcc cgagggcgac cggttcgtct gcgtggccgg cagccagccg
     7321 atggacgccg ccgtgcccga agcccggcgc caggccgagg tcggcacgat cctggaaacc
     7381 tgccccggcg cggccgccac ggtttacgaa gtggccgggc ggctggttga acaaggcggg
     7441 gcggcgctgt tcatcgatta cggccacgat gccccgcgcg acggatcgac cctgcaggcc
     7501 gtgcgcgatc acctgaaggt ggaccctttc gtgggcccgg gcgaggccga tctgaccgcc
     7561 catgtcgatt tcgccaccct ggccccgatc gccctgtcgc gcgggtgcaa gtggctgggc
     7621 acggttccgc agggacgctg gctgcgcaac ctggggatcg atgcccgcgc gcaggccctg
     7681 gccgaacgcg cgccccagca cgccgccgcg atcgagaccg cgaaggagcg gctggtcggc
     7741 gaagggcaga tgggggtgct gttcaaggtg atggggctgt gttctccggc ctggcccggc
     7801 ggggccgggt tctaagccgc gcccttccga acctgcacct ctgtgcgctt gccagcgcgg
     7861 gccacttgcc tctaagcctt gggcagaagc cggcgggttt ggaccggcct gatgggagag
     7921 cacatggaca agccgcatgt ccgccggccg accttgctgt cgcgcgtggc gaagcaggtc
     7981 ttgctctggt tttaccacgg aaagggctgg cggctgttcg gcggcctgcc ggaaacccgc
     8041 aagttcgtga tcgccggcgc gccgcacacc tccaattggg acttcgtgtt cttcaccggc
     8101 gccacccacg aaatgggcat ccagcccaac ttcatgggca agcacaccct gttcaaatgg
     8161 ccgctgaagc gcttcatgga agacatgggc gggatcccgg tcgaccggac caagcgggcc
     8221 aactatgtcg agcagatcgc cgccgagttc gcccggcggg acgagctggc gctggtgatc
     8281 gccgccgaag gcacgcgcag caccgacgga tcgtggaaat cgggctttta tcacatcgcc
     8341 caggcggccg gcgtgccgat cgtgccgacc tgggtctgca acgcgaccaa gcggcttggc
     8401 tttggcccgg cgctgatgcc cagcggcgac tatccagccg acctggccaa gatcgccgcc
     8461 tatctgcggt cggtcctgcc cgatgagcca cgtttcaaag tgctggaagc gcaggcaatg
     8521 cgtctgctga agggagagca ggatgtttga ggcgctgctg atcaatgccg cgatcctgat
     8581 cgcgaccgtg ctggtgctgt gggcgatctc ggtccgcatc aacgatgtct cgttcatcga
     8641 ttcgttctgg ggtgccggga tggggctgat ggccctgatc agctggatgc aggtggagcc
     8701 gctgcccggt gcgctgggca cgctgatcat ggcgatgacc tgcgcctggg gctttcgcct
     8761 ggggatctac ctgctgcgcc gctggcgccg cgagggcgag gacccgcgct atgtccggat
     8821 cctgcgcaag gaccgcgagg ccggccgctt tgcctttgcc gcgctgacca aggtctggct
     8881 gggccaggcc tttctgctgt tcctggtctc gagcccggcc caggtggcga tcctggccag
     8941 cggcgcaccc gcgccggtgc cgccgctggc ctgggccggt ctggcgctct acctggtcgg
     9001 gatcttcttc gaatgggtcg gcgattggca gctggcccgg ttcaaggcca acaaggccaa
     9061 cgagggccgg atcctcgata ccgggctgtg gcgctacacc cgccatccca actacttcgg
     9121 cgattgcctg gcctggtggg gtatctggct gtgctgcgcc ggggccggct ggccatacgc
     9181 gctggcgacc ctgccggggc cgctgttcct gaccttcacc ctgaccaagt ggtccggcgc
     9241 ggcgctgctg gaagcgggca tgaagaagaa gcgcggcgac aagtacgctg actatatcgc
     9301 ccgcacctcg gccttcgtgc cctggccgcc caagcagctc tgacgcctag ttcccgactg
     9361 ccaccgcgcg cgtctcgctc ggcacggcgc ggggcgcgtc gcccttcagc caggcctcca
     9421 acgcgtcgag atcgctgatc ccgacctgcg cggtgcgctg tccggcgaag acggtgaagg
     9481 catcgccgcc ctgggccagg aacgaattgg tcgtcacccg gtacatcccg ttcgggtcga
     9541 tcggctgccc gttcagccgc atggccacga tccgctgccc gaccgggcgc gacttgtcat
     9601 agctgtaggt gaacccggcc gaggggatca gcggctgttc cggtccgttc ccgtcaaagc
     9661 tctgctccag cacggccttc agttcggccc cggtcaggct ctgggtgatc agcgtgttgt
     9721 tgaacggctg cgcggcatag agatcgccga aggtcagcag cccgccttcc cccgggttca
     9781 gcggcgcgcg cacgccgaag gggttcatga aggcgatctg ggccccggcg atccgggtcg
     9841 cttccagctg cccgtcggcg atcagcatgc ccagcgccca gcccgaagcc gggccggcca
     9901 gcttgcccgc cgggcgcgag gagaaggcct tggccgcatc ggtgtagcgc tggacatagg
     9961 cggcgacatc ggcgcgcggg gtgaacagcg ggaacagcgc ggtgttcgcc acctgcccgc
    10021 cgcgcgggct ggcatagggc agcgactggg cgacaacctg ccgcgcccgc cgggccacca
    10081 cccgcccggc caccggatcg atatccagcg cgatgtcggt caccatcatc ccgttctttt
    10141 ccgcgctggt cagcaggaac ggcttggccg ggttgtaggc cgaatagtcg cagatatagg
    10201 cgcggtgggt atggcccgag acgaccagat cgacccgcgg gtcgagccgg tcgaggatcg
    10261 ggcggatttc gccggtgaag ccggtgcagc tgttcggatc gccgccttcg ccctgctggc
    10321 cgccctggtg gatcagcacg acgatcgcgt cggcgc
//
LOCUS       DASUDV010000295        10094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_230481,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000295 DASUDV010000000
VERSION     DASUDV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230481"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFV30_06420"
     CDS             <1..438
                     /locus_tag="VFV30_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4847759.1"
                     /translation="AYVEGWALYAETLGYEMGFYADPMQHWGTLDDEMLRAMRLVVDT
                     GLHAKGWTRDQAIEFMLANSGMGRSDATAEVERYIVWPGQALGYKIGALTIQRLRRKA
                     EAALGKRFDIRAFHDEVLGSGALPLPVLEAKIDRWIAGQTAQG"
     gene            491..877
                     /locus_tag="VFV30_06425"
     CDS             491..877
                     /locus_tag="VFV30_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4847760.1"
                     /translation="MSAPAAIPLPRTGKRCHARLRVRLPAKLITLDGDYRVVLCDLSQ
                     SGARIGQPGLPLLQGQAILLWDKYEAFGRFAWCRAGLAGLAFLDPIPPAWVLATRDLD
                     DAHHLPDDSELQRRHAREWVTGRNRV"
     gene            complement(882..2474)
                     /locus_tag="VFV30_06430"
     CDS             complement(882..2474)
                     /locus_tag="VFV30_06430"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX4847761.1"
                     /translation="MTDEALLTAAQVSKAWPFEEARKLLKRYPDGKPDGAPVLFETGY
                     GPSGLPHIGTFQEVLRTTLVRRAYETLTGGAPTRLVAFSDDMDGLRKVPDNVPNQGLL
                     TAYLGHPLSRIPDPFGKYESFAHHNNAMLREFLDRFGFDYEFVSSSDQYNSGAFDEAL
                     RGILRNWQAIMDIMLPTLREERRQTYSPVLPVSPKTHQVLQVPVEVVDAEAGLVRFED
                     HGEMVESCIFGGNSKLQWKVDWAMRWVALGVDYEMCGKDLTDSVRESGRITRALGGRP
                     PEGLIYELFLDEEGQKISKSKGNGLTIDQWLTYGSEESLGLFLFREPKSAKSLHTGII
                     AKNVDEYWQFREKLPSQPIEQQLGNPVWHLLRANGYHGGDGDKLPVTYGLLLNLVGVL
                     GAQATREQIWSYLGNYVPDADPAAHPELDKLVTNALAYNRDLIAPTLKRRKPEGGEVA
                     ALAALDEELAATSDDATAEELQNIVYEIGKDPHYGFEQLRDWFKCLYETLLGSSQGPR
                     MGSFIALYGIANTRALIADALS"
     gene            complement(2521..3321)
                     /locus_tag="VFV30_06435"
     CDS             complement(2521..3321)
                     /locus_tag="VFV30_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847762.1"
                     /translation="MVLRPFLLTLAALLLGGAAAAPTFLREQKAVTVDGRAEQWQLVW
                     EGRPKPICGPQDVEMAITCPCTGFAYGELGRLSLVRKQGGQVVDRLALGPYFDELPAD
                     NSKGLAAMQWRPMDPRDFDEMADGGATPAQLAQIRQRPGPRVMRMGDYDRDGNASEFL
                     VQVSAGPCGHTEYMLFGVSRANPRLHAFGSADHPGDPLVLPGQAWQALLTRPGPSKVT
                     AWGCGDHGSDVRQELVLSSAKGAIRVRQRTWSCPEDGSAERLIREAVL"
     gene            3427..5130
                     /locus_tag="VFV30_06440"
     CDS             3427..5130
                     /locus_tag="VFV30_06440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX4847763.1"
                     /translation="MIARLAKLALAACAALTLGAAGTAKPPAPPATLWHGGPIITMDG
                     ATPATVQAVVERGGRIVFAGSLARARKLAGPAATDRDLKGQTLLPGFIDAHSHFSMLM
                     QIETGIDLDAGDAPPQDIPALIALLRQGVAAKGIGETDWIIGWNYKDAALAEKRHVTR
                     DDLDAAFPGRKVVLIHFTGHGLVASSPALEAAGVKDDTPNPPGGLFLRDAKGRPNGVV
                     FEAALLPFYKALPRPTPAQMLAALEATQMGYASKGFTHAQDGATQIGEMKFLLSPAAR
                     ERMKIDLAILPSFAGLDALLAMPDLKFGQYQGRTKVQGVKFVLDGSPQARTAYFTRAY
                     ANGSPEGHHPWHGQPIVSQDEFIKQARLVHARGWQLFVHANGDAAIDMAIKGFDALGI
                     KAADNRRPVVIHSQFQRPDHLPAYARIGVGPAYFSNHTLYFADIHRSNFPAEVVAFIS
                     PFKAAMKAGLHPSNHSDAPVTRLDPFVQLWSSMTRASLSGVVSGPDQRLSAYEALQML
                     TTGPAWQVFEENRKGRIKAGLLADFVVLDRNPLTTPVDGIRSIRVVETVKEGRTIWKR
                     S"
     gene            complement(5127..5681)
                     /locus_tag="VFV30_06445"
     CDS             complement(5127..5681)
                     /locus_tag="VFV30_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX4847764.1"
                     /translation="MADKVYLTADRLLEDSFALANLVLESGFRPTHIVGIWRGGAPVG
                     IAVQELLAYRGVETDHIAIRTASYTGIDQQAKEVRVYALGYLIDVLDPEDRLLVIDDV
                     FDTGRSIEAFLAELKARCRHNTPETIRIATAYYKPSRNQTSLKPDYFVHQTEEWLVFP
                     HEICGLTEDEIRQHKPGARLILGD"
     gene            complement(5671..6690)
                     /locus_tag="VFV30_06450"
     CDS             complement(5671..6690)
                     /locus_tag="VFV30_06450"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX4847765.1"
                     /translation="MPNPSRAALIDRLPKAELHLHIEGSLEPELLFALATRNGVAIPF
                     ASVEDVRAAYSFSNLQDFLDIYYQGMSVLLTEQDFYDLTWAYLERAHADTVRHVEIFF
                     DPQGHTERGVAFETVLNGIERALKDGETQLGISYRLIMCFLRHLSEEDAFATLDQALP
                     HLERIHGVGLDSSEVGHPPAKFQRVFARARELGLHVTAHAGEEGPPEYVHEALDLLQV
                     ERIDHGNRALEDAALVRRIADEGLTLTVCPLSNLRLCVIKDIAESPVKRMLEMGLKAT
                     VNSDDPAYFGGYINANFHAVAEALDLTEAEIVRLAENSFTGSFLPMADQIQHLAEIAE
                     VAGGR"
     gene            complement(6730..7509)
                     /locus_tag="VFV30_06455"
     CDS             complement(6730..7509)
                     /locus_tag="VFV30_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847766.1"
                     /translation="MAYGYEDPDRDGLGLGAKIAIGLGVLVGLPLIAAFAYGIYVTLL
                     MTPEQKFDRMLEAEPEARIFIDPLKKHYPEEYEDLRGSVLVALQGTNGELNGRLASMG
                     FMNGFVVRHGYEAALAPDADLKPLLDAQTNAFAVFAANPNACESAIRQVPLADDKMKK
                     QDKVTLIRFSGQFVKTAAAGRDRPVRRDRAGPEDQTALIEAMRAEGVSPSDLRDLDPA
                     GGAREEAVRRCGVLHKTLRAVQKLPPERSVRIYALLLGSSR"
     gene            complement(7538..8029)
                     /locus_tag="VFV30_06460"
     CDS             complement(7538..8029)
                     /locus_tag="VFV30_06460"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX4847767.1"
                     /translation="MFQRKAKLGATGFASLMMLAGFPATSAEPQPVFPQMLPGRYEEA
                     KQMVDIVGNVTAGARDRAVSKPPEIGTVCYSKAMAAEKSLFENRFGQTCEVRTAEVNG
                     TAVRGTLFCKNPGMDGIMTYEGTASETAADLRVTYTFGSGKSGAETQFIASIKLKRLS
                     PDC"
     gene            8149..>10094
                     /locus_tag="VFV30_06465"
     CDS             8149..>10094
                     /locus_tag="VFV30_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX4847768.1"
                     /translation="MSDALPLPALHASHGGCWLRDGHGQTRTVSKGDAIVAAADTPLL
                     LLNAPLVATRLGYPDLSGLDLLELFAFVHPARFVVPTAKGLAHALDLPEPEGDDGVPA
                     LLQQAAEALLSVCQQDGWAEREGAWSALQNLTRLRWAWAPLLAARIAAPAKAERWLFS
                     RLPEWEDAPERPQPAQVVLRPDDVNARLARLTGDGAESRRAQAAYAQEAAHVFEPRRR
                     EGLPHLLLAQAGTGIGKTLGYLAPASLWAEQSGGTVWVSTYTKALQRQLRRESRRAWP
                     ATRPDGTPPVVVRKGRENYLCLLNLEDALQGGFTGRPAIMAQLVARWAAYSQDGDMIG
                     GDLPGWLGTLFRQRGIKALTDQRGECVYAGCPHYRKCFIERAARASAQADLVIANHAL
                     VMVNAARGRDTAQRPTRIVFDEGHHVFEAADSTFAAALTGAETIELRRWVIGPERGSK
                     GRRRGLSARLADLASYDEAGGKAIAAARHAAEALPGDGWLQRLHEGSPSGPLEELLAA
                     VRATVYARDESGGQEAGYGLETEAAQLDGDFIERAGAAQAALAELRSPLIRLGVRLEA
                     LLAEPPDWLDGPGRARIEGARHSLAWRIDLLAAWEALLERLGGPADPEFVDWLAVERA
                     EAREFDIGIHRRWLDPMKPFARTVL"
BASE COUNT         1563 a         3491 c         3392 g         1648 t
ORIGIN      
        1 gcctatgtcg aaggctgggc gctctatgcc gaaacgctcg gctacgagat gggcttctac
       61 gcagacccga tgcagcactg gggcacgctg gacgacgaga tgctgcgcgc gatgcggctg
      121 gtggtcgaca ccgggcttca cgccaagggc tggacccgcg accaggcgat cgagttcatg
      181 ctggccaatt ccggcatggg ccgcagcgac gcgaccgccg aggtggagcg ctacatcgtc
      241 tggccgggcc aggcgctggg ctacaagatc ggcgcgctga cgatccagcg cctgcgccgc
      301 aaggccgagg ccgcgcttgg caagcgcttc gacatccgcg ccttccacga cgaggtgctg
      361 ggatcgggcg cgctgcccct gccggtgctg gaagccaaga tcgaccgctg gatcgccggg
      421 cagaccgctc agggatagcc cctaggccgc tggcagcggt gcattagccg taagcgggca
      481 tggtggcggc atgtccgctc ctgccgccat tccccttccc cgtaccggca agcgctgcca
      541 cgcgcggctg cgggtgcgcc tgccggccaa gctgatcacg ctggacggcg attaccgggt
      601 ggtgctgtgc gacctgtcgc aatccggcgc gcggatcggc cagccgggcc tgccgctgct
      661 gcagggccag gcgatcctgc tgtgggacaa gtacgaggcc ttcggccgct tcgcctggtg
      721 ccgcgcgggc ctggcgggcc ttgcctttct cgacccgatc ccgccggcct gggtcctcgc
      781 cacgcgcgat ctggacgacg cccatcacct gccggacgac agcgaactgc agcgccgcca
      841 tgcccgcgaa tgggtcactg gccggaaccg ggtctgacgg cttagctgag cgcatcggcg
      901 atcagcgcgc gggtgttggc aatgccgtag agcgcgatga agctgcccat gcgcgggccc
      961 tggctggagc caagcagcgt ctcgtacagg cacttgaacc agtcgcgcag ctgctcgaac
     1021 ccgtagtgcg ggtccttgcc gatctcgtag acgatgttct gcagctcttc cgccgtggca
     1081 tcgtcgctgg tcgcggccag ttcctcgtcc agcgcggcca gcgccgccac ttccccgccc
     1141 tcgggcttgc ggcgcttcag cgtgggcgca atcagatcac ggttgtaggc cagcgcgttg
     1201 gtgaccagct tgtcgagctc cgggtgcgcg gccggatcgg catcgggcac atagttgcca
     1261 aggtaggacc agatctgctc gcgcgtggcc tgggccccca gcacgccaac caggttgagc
     1321 aacaggccat aggtgactgg cagcttgtcg ccatcgccgc cgtggtagcc gttggcgcgc
     1381 agcaggtgcc agaccgggtt gcccagctgc tgctcgatcg gctggctggg aagcttttcg
     1441 cggaactgcc agtattcatc gacgttcttg gcgatgatcc cggtgtgcag gctcttggcg
     1501 cttttcggct cgcggaacag gaacagcccc aggctttcct cgctgccata ggtcagccat
     1561 tggtcgatgg tcagtccgtt gcccttggac ttggagatct tctggccttc ctcgtcgagg
     1621 aacagctcat agatcagccc ttccggcggg cggccgccca gcgcgcgggt gatccgcccg
     1681 ctttcgcgca cggaatcggt caggtccttg ccgcacattt cgtaatcgac gccgagggca
     1741 acccagcgca tcgcccagtc gaccttccac tgcagcttgg aattgccgcc gaagatgcag
     1801 ctttccacca tctcgccgtg atcctcgaac cggaccaggc cggcttcggc atcgaccacc
     1861 tcgaccggca cctgcagcac ctgatgggtc ttggggctga ccggcaggac cggcgaatag
     1921 gtctgccggc gctcctcgcg cagggtcggc agcatgatgt ccatgatcgc ctgccagttg
     1981 cgcaggatgc cgcgcagcgc ctcgtcaaag gcgccggaat tgtactggtc gctggacgag
     2041 acgaattcgt aatcgaaccc gaaccggtcg aggaattcgc gcagcatcgc gttgttgtga
     2101 tgggcgaaac tctcgtactt gccgaaggga tcggggatcc ggctgagcgg atggccgaga
     2161 taggcggtca gcagcccctg gttggggaca ttgtccggca ccttgcgcag accatccatg
     2221 tcgtcgctga aggcgaccag gcgcgtcggt gcgccgccgg tcagcgtctc ataggcgcgg
     2281 cggaccagcg tggtgcgcag cacttcctgg aaggtgccga tatgcggcag gcccgagggg
     2341 ccatagcccg tctcgaacag caccggcgcc ccatccggtt tgccgtccgg ataccgcttc
     2401 agcagcttgc gcgcttcctc gaagggccaa gccttggaaa cctgtgcggc ggtaaggagt
     2461 gcttcgtcgg tcatagtgcc cgcgcctttg cgcgaatggg ccggtttcgc aagtgcgaag
     2521 tcaaagcacc gcttcgcgga tcaagcgttc ggcgctgcca tcctccgggc aggaccaggt
     2581 tcgctgccgc acccggatcg cgcctttggc cgacgacagc accagttcct gccgcacgtc
     2641 cgagccgtga tcgccgcagc cccaggcggt gactttcgac ggaccgggcc gggtcagcag
     2701 cgcttgccag gcctgcccgg gcaggaccag cggatcgccc ggatggtccg cgctgccaaa
     2761 ggcgtggagc ctgggattgg cgcggctcac cccgaacagc atgtattccg tgtggccgca
     2821 gggcccggcc gaaacctgga ccaggaactc gctggcgttg ccgtcgcggt catagtcccc
     2881 catccgcatc acccgcggcc ccggccgctg gcggatctgc gccagctgcg ccggggtggc
     2941 gccgccatcg gccatttcgt caaaatcgcg cgggtccatc ggccgccact gcatcgccgc
     3001 cagccccttg gaattgtcgg ccggcagctc gtcgaaatag gggcccagcg ccagccggtc
     3061 gaccacctgg ccgccctgct tgcggaccag gctgagccgg ccgagctcgc cataggcaaa
     3121 gccggtgcac gggcaggtta tcgccatttc gacatcctgc ggaccgcaga tcggcttggg
     3181 ccgcccttcc cagaccagct gccactgctc ggcccggcca tcgacggtga cggccttctg
     3241 ctcgcgcagg aacgtggggg ccgcggccgc accgcccagc agcagcgcgg ccaatgtcag
     3301 caggaacggg cgcaggacca tgccggtgtt caaggcccaa ttgcgccgcg gtgcaagcgc
     3361 caagttgcac ccggccccgg ccctgtgcat ggtggcgcaa acaggatcct ggaggggatg
     3421 aggacgatga ttgcacgcct ggcaaagctg gcgctggccg cctgcgcggc gttgaccctt
     3481 ggtgcggcgg ggacggccaa gccgccggcc ccgcccgcga ccctgtggca cggcggcccg
     3541 atcatcacca tggacggggc cacgcccgcg acggtgcagg ccgtggtcga acgcggcggc
     3601 aggatcgtct ttgccggaag cctggcccgg gcgcgcaagc tggccggccc cgccgcgacc
     3661 gaccgcgacc tgaaggggca gaccctgctg cccggcttta tcgacgcgca ctcgcacttt
     3721 tcgatgctga tgcagatcga aaccgggatc gacctggatg ccggcgatgc cccgccgcag
     3781 gacattcccg ccctgatcgc cctgctccgg caaggggtgg cggccaaggg cattggcgag
     3841 acggactgga tcatcggctg gaattacaag gacgcggcgc tggccgaaaa gcgccacgtc
     3901 acccgcgacg atctcgatgc ggcctttccg ggccgcaagg tggtgctgat ccacttcacc
     3961 ggccacggcc ttgtcgccag cagccccgcg ttggaagcgg ccggggtgaa ggacgatacg
     4021 cccaacccgc ccggcggcct gttcctgcgc gatgctaagg ggcgccccaa cggcgtggtc
     4081 ttcgaagcgg cgctgttgcc gttctacaag gccctgcccc gcccgacccc ggcgcaaatg
     4141 ctggccgcgc tggaggcgac gcagatgggc tatgccagca agggcttcac ccacgcgcag
     4201 gacggcgcga cccagatcgg ggaaatgaag ttcctgctca gccccgccgc acgcgagcgg
     4261 atgaagatcg accttgcgat cctgccgagc tttgccgggc tggatgccct gctcgccatg
     4321 ccggatctga agttcggcca gtaccaaggg cggaccaagg ttcagggggt gaagttcgtg
     4381 ctcgatggct caccccaggc ccgcaccgcc tatttcacgc gggcctatgc caacggctcg
     4441 cccgaggggc atcatccctg gcacggccag ccgatcgtct cgcaggacga gttcatcaag
     4501 caggctcggc tggtccacgc gcgtggctgg cagctgttcg tccacgccaa cggcgatgcg
     4561 gcgatcgaca tggcgatcaa gggctttgac gcgctgggga tcaaggccgc cgacaaccgc
     4621 cgcccggtgg tgatccattc gcagttccag cggcccgatc acttgccggc ctatgcccgg
     4681 atcggcgtgg ggccggccta tttctccaac cacacgctct atttcgcgga catccaccgc
     4741 agcaatttcc cggccgaggt ggtcgccttc atcagcccgt tcaaggcggc gatgaaggcg
     4801 gggctgcacc cctccaacca ttccgacgcg ccggtcaccc ggctcgatcc ctttgtccag
     4861 ctgtggtcca gcatgacccg cgcctcgctc tcgggcgtgg tcagcggccc cgaccagcgc
     4921 ctcagcgcct acgaagccct gcagatgctg accaccggcc ccgcctggca agtgttcgag
     4981 gaaaaccgca agggccggat caaggccggg ctgctcgccg atttcgtggt gctggaccgc
     5041 aatccgctga ccacgccggt ggacggcatc cgctcgatcc gggtggtgga aacggtcaag
     5101 gaaggccgga cgatctggaa gcggagctag tcgcccaaga tcaggcgcgc ccccggcttg
     5161 tgctggcgga tctcatcctc ggtcaggccg cagatctcat gcgggaagac cagccattcc
     5221 tcggtctgat ggacgaagta atcgggcttc agcgatgtct ggttgcggct cggcttgtaa
     5281 taggccgtcg cgatgcggat cgtttcgggg gtgttgtggc ggcagcgggc cttgagctcg
     5341 gccaggaacg cctcgatcga gcggccggta tcgaacacgt cgtcgatcac cagcagccgg
     5401 tcctccggat cgagcacgtc gatcagatag cccagcgcat agacccgcac ttccttggcc
     5461 tgctggtcga tcccggtgta gctggcggtg cggatcgcga tgtgatcggt ttccaccccg
     5521 cgataggcga gcagttcctg caccgcgatg ccgaccggcg ccccgccgcg ccagatgcca
     5581 acgatgtggg ttggccgaaa gcccgattcg agcaccagat tggccagcgc gaagctgtct
     5641 tccagcaggc ggtcggcggt gaggtagacc ttatcggcca ccggccacct ccgcgatctc
     5701 ggccaggtgc tggatctggt cggccatcgg caggaacgat ccggtgaagc tgttctcggc
     5761 cagcctgacg atctccgctt cggtcaggtc cagcgcctcg gcgacggcgt ggaaattggc
     5821 gttgatgtag ccgccgaaat aggccggatc gtcggaattg accgtggcct tcaggcccat
     5881 ttcgagcatc cgcttgaccg ggctttcggc aatgtccttg atcacgcaga gccgcaggtt
     5941 ggacagcggg cagaccgtca gcgtcaggcc ctcgtcggcg atgcggcgaa ccagcgcggc
     6001 atcctccagc gcgcggttgc cgtggtcgat ccgctccacc tgcaagaggt ccagcgcctc
     6061 gtgcacgtat tcaggcgggc cttcctctcc agcatgggcg gtcacgtgca gacccagttc
     6121 ccgcgcccgg gcgaacaccc gctggaactt cgccggtggg tggccgacct ccgaggaatc
     6181 gaggccgacg ccgtggatcc gctccagatg cggcagggcc tggtccagcg tggcgaaggc
     6241 gtcctcctcg ctgaggtgcc gcaggaagca cataatcagg cggtagctga tccccagctg
     6301 cgtctcgccg tccttcagcg cgcgttcgat accgttcagc acggtttcaa acgccacgcc
     6361 gcgctcggta tggccctggg gatcgaagaa gatctcgaca tggcggacgg tgtcggcatg
     6421 ggcccgttcc agataggccc aggtcaggtc atagaagtcc tgctcggtca gcagaaccga
     6481 catgccctgg tagtagatat ccagaaagtc ctgcaggttg gagaacgaat aggccgcccg
     6541 cacgtcctcc accgaagcga acgggatcgc caccccgttg cgtgtcgcca gggcgaacag
     6601 cagctccggc tccagcgagc cttcgatgtg gaggtggagt tcggccttgg gcaggcggtc
     6661 gatcagcgcg gcgcgggacg gattcggcat gggcggacgc tggcccagcc gctctggcgg
     6721 gtcaaccgct cagcggctcg atcccagcag caacgcataa atccgcaccg accgctcggg
     6781 cggaagcttt tgcaccgcgc ggagcgtttt gtggagcacg ccgcagcgcc gcaccgcctc
     6841 ttcgcgcgcg ccgcccgccg ggtcgaggtc gcgcagatcg gacgggctca cgccctcagc
     6901 ccgcatcgcc tcgatcagcg cggtctggtc ctctggccct gccctgtcgc gccgcaccgg
     6961 acggtcccgt ccggcggcgg cggtcttcac gaactggccg gaaaagcgga tcagcgtgac
     7021 cttgtcctgc ttcttcatct tgtcatcggc cagcggcact tgccggatcg cgctttcgca
     7081 agcgttgggg ttggcggcaa acaccgcgaa agcgttggtc tgggcatcga gcagcggctt
     7141 cagatccgcg tcgggggcga gcgccgcctc gtacccgtgg cgcaccacga aaccgttcat
     7201 gaagcccatc gaggcgagtc gcccgttcag ttccccgttg gtgccctgca gggcgaccag
     7261 caccgatccg cgcagatcct cgtactcttc gggatagtgc tttttcagcg ggtcgatgaa
     7321 gatccgtgcc tcgggctcgg cctccagcat ccggtcaaac ttctgctcgg gcgtcatcag
     7381 cagggtgacg tagatgccgt aagcaaaggc cgcgatcagc ggcagaccga ccagcacccc
     7441 cagcccgatc gcgatcttcg cccccaggcc cagcccgtcg cgatccggat cctcatagcc
     7501 ataggccatc gccgttcccc cttcctctcc ggccggatca gcagtccggc gagagccgct
     7561 tgagcttgat ggaggcgatg aactgcgttt cagccccgga cttgccagat ccaaaggtgt
     7621 aggtgacccg caggtccgcc gcggtttcgc tggcggtacc ctcgtaggtc attatcccat
     7681 ccatcccggg gttcttgcag aagagcgtgc cgcgcaccgc cgtgccgttc acttcggcag
     7741 tgcggacttc gcaggtctgg ccaaaccggt tttcgaacag tgacttttcc gcagccatcg
     7801 ccttgctgta gcacaccgtg ccgatttcgg gcggcttgct gaccgcccgg tcgcgggccc
     7861 cggccgtgac attgccgacg atgtcgacca tctgcttcgc ttcctcgtag cggccgggaa
     7921 gcatctgcgg gaacacgggc tgcggttcgg cgctggtcgc gggaaagccc gccagcatca
     7981 tcaacgacgc gaaccccgtg gcccccagct ttgccttgcg ttggaacatc ttgcgctttc
     8041 ccctctccgc catcgcgttg tagtcaacgc caagtcgcgc cgtggcacaa ggggaaagtg
     8101 gcgcgcaccg ccttgcctct tgttccattt ccgttctaac gctgcgcggt gagcgacgcc
     8161 ctgccccttc ccgcgctgca cgccagccac ggcggctgct ggctgcgcga tggccacggc
     8221 cagacccgca cggtgagcaa gggcgacgcg atcgtcgccg cggccgacac gccgctgctg
     8281 ctgctcaacg cgccgctggt cgccacccgg ctgggctatc cggacctgtc ggggctggac
     8341 ctgctggagc tgttcgcgtt cgtccatccg gcccgcttcg tggtgccgac cgccaagggc
     8401 ctggcccatg cgctggacct gccggagccc gagggcgatg acggtgtccc cgcgctgctc
     8461 cagcaggccg ccgaagcgct tctgtccgtc tgccagcaag acggctgggc ggagcgcgaa
     8521 ggagcctggt cggccttgca gaacctcacc cggctgcgct gggcctgggc cccgctgctc
     8581 gccgcgcgaa tcgcggcacc ggccaaggcc gaacgctggc tgttttcccg cctgccggaa
     8641 tgggaggacg cgcccgagcg gccccagccg gcccaggtcg tgctgcgtcc ggacgatgtc
     8701 aacgcccggc tggcgcggct gaccggcgac ggcgcggaaa gccggcgcgc ccaggccgcc
     8761 tatgcgcagg aagccgccca cgtgttcgaa ccgcgcaggc gcgaaggact gccgcacctg
     8821 ctcctcgctc aggccgggac cgggatcggc aagacgctgg gctatctcgc cccggcctcg
     8881 ctgtgggcgg agcaatcggg cggaacggtc tgggtctcca cctataccaa ggcgctgcag
     8941 cgccagctgc gccgcgaaag ccgccgggcc tggccggcaa cccgccccga tggcaccccg
     9001 ccggtggtgg tgcgcaaggg gcgggagaat tacctgtgcc tgctgaacct ggaagacgcc
     9061 ttgcagggtg gtttcaccgg tcgcccggcg atcatggcgc agctggtggc ccgctgggcg
     9121 gcctacagcc aggacggcga catgatcggc ggggacctgc ccggctggct cggcaccctg
     9181 ttccgccagc gcgggatcaa ggcgctgacc gaccagcgcg gcgaatgcgt ctatgccggg
     9241 tgcccgcatt accggaagtg cttcatcgaa cgcgcggcgc gggccagcgc ccaggccgat
     9301 ctggtgatcg ccaaccacgc gctggtgatg gtcaacgccg cgcgcggccg cgacaccgcc
     9361 cagcgcccga ctagaatcgt gttcgacgaa ggccaccacg tgttcgaagc ggcggattcg
     9421 accttcgccg ccgcgctgac cggggccgag acgatcgaac tgcgccgctg ggtgatcggg
     9481 ccggaacgcg gctccaaagg ccgccgccgg ggcctgtccg cccggctggc cgaccttgcc
     9541 agctatgacg aggccggcgg caaggcgatc gctgccgcgc gccacgcggc cgaggcgctg
     9601 cccggcgacg gctggctcca gcgcctgcac gagggttcgc cctccggccc gctggaggag
     9661 ctgctcgccg ccgtccgtgc cacggtctac gcccgcgacg aaagcggcgg gcaggaagcg
     9721 ggctatggcc tggaaaccga ggccgcgcag ctcgacggcg atttcatcga gcgcgccggg
     9781 gcggcccagg cagcgctggc cgaactgcgc agcccgctga tccgtctggg cgtgcggctg
     9841 gaggcgctgc tggccgaacc gcccgactgg ctcgacgggc ccggccgcgc ccggatcgaa
     9901 ggcgcgcgcc attcgctcgc ctggcggatt gacctgctgg ccgcgtggga ggccctgctg
     9961 gaacggcttg gcggcccggc cgatcccgaa ttcgtcgact ggctggcggt ggaacgggcc
    10021 gaggcgcgcg aattcgacat cggcatccac cgccgctggc tcgatccgat gaagcccttt
    10081 gcccgcaccg tgct
//
LOCUS       DASUDV010000296         4435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1312819,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000296 DASUDV010000000
VERSION     DASUDV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312819"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VFV30_06470"
     CDS             complement(<1..85)
                     /locus_tag="VFV30_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX4847769.1"
                     /translation="MRIDRLLWFLRLAKTRSLAQALVGEGHI"
     gene            complement(85..2616)
                     /locus_tag="VFV30_06475"
     CDS             complement(85..2616)
                     /locus_tag="VFV30_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX4847770.1"
                     /translation="MTRRASAHSDSRIKAVLGPTNTGKTHLAIERLCAHSSGAIGFPL
                     RLLAREVYDKVCKIKGVDRVALITGEERIEPRHARWLLCTAEAMPVGADLSFVGIDEA
                     QLGADRERGHIFTDRLLHARGRDETMILGSATLEPLVKALVPQAEIVTRPRFSTLSHV
                     GAKKLSRVPPRSAIVAFSAEQVYAMAEMLRRFRGGAAVVMGALSPETRNRQVELYQAG
                     EVDYLVATDAIGMGLNLDIEHVAFAGLSKFDGQRQRRLTPAEMAQIAGRAGRHQKDGS
                     FGTLAGPGGREAEFSDEEIYAIEEHRFAPLTRLFWREAEPDCTSLRSLIADLERAPDR
                     PELAPAPEAIDLAVIKRLAEDPEIAGSLRGEAAVRRFREVCSLPDFRQHGAETHSRFV
                     ARLWQDLRRGQLGQDFVARAIADLDNPAGDIDTLQGRIASVRSWAYIAQRPDWVLARE
                     EMAARARAAEARLSDALHARLTERFVNRRTAVLMKKLGSDASLLPVRLVDDEVLVDGE
                     HIGVLAGFRLRIDPQARLADRKLLLAAAERHLPALLCERAARLGEALAAASPDLGLDG
                     ARICWEGEVVAQLAKGRGLLSPRIELDAALGALPALARPSLQAGLEQWLGHALAALAP
                     LTALEAASRQEQAGPELRALLIRLVEAGGTLERAGSGLERLNETQRGFMRRLGVRIGA
                     LDLFMPAMLKAAALAAWRTLLAASGRAVAAPVPGMPPVIALAARSATPPGYRSLGRQA
                     IRLDIAEKLLRAAHEARVAAKGKGFVIDPALAISTGLGTAAFAQLLRLAGFTASVHRP
                     LPEGAFGPPVPPRWRWRPPRREVEAAGAARAVPGTAFAALAALVS"
     gene            2778..4352
                     /locus_tag="VFV30_06480"
     CDS             2778..4352
                     /locus_tag="VFV30_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4847771.1"
                     /translation="MTKRGEQAGWAAGTGMARAMAIPAGAIVLAPTRAERLMRLREVL
                     AQRFAHLEISYDLAEDIGSAPWFRGLGLMLGLGLFASLFWHDSGAIAAPPGMVLDQDQ
                     RDEFRSQMIMPLALGADSGRRMGATSALVALAAAPERPTLQLVATLGQGDSLSRMLVR
                     AGVDSGDALTAAGLVAMVMPKGEVAPGTQADLTLGKIDPATGTRPLERMAMRARFDLD
                     LALTRTPSGQLTIQPHAIAVDTTPLRIRGTVGTSIYRSARAAGAPIEAIQQYLQVLDQ
                     HLSLDTQIANGDTFDLIVSYKRSARGEAQVGELLFAGLERDGKPQAQLLRWGKDGQFF
                     EASGMGAQRTGLIMPVVGRITSNFGARRHPVLGYTRMHAGVDFGAAHGSPIYAVGDAR
                     VIYSGWHGGHGNYVKLDHGGGFATAYAHMSRIAVSSGSVVRTGQIIGYVGSTGLSTGP
                     HLHYELYRNGQAVNPLSVRFTVSSQVDPAELKAYKARMAALKAVKPGAAMNSLAPRQA
                     SVAGIGPQREIDRLAN"
BASE COUNT          674 a         1537 c         1538 g          686 t
ORIGIN      
        1 ggatatggcc ttcgccgacc agcgcctggg caaggctgcg ggtcttggcc agccgcagga
       61 accacaggag cctgtcgatc cgcatcagct gaccagcgcg gccagcgcgg caaaggccgt
      121 tcccggcacc gcccttgccg cccctgcggc ttcaacctcg cgccgcggcg ggcgccagcg
      181 ccagcgcggc ggcaccgggg ggccgaaggc gccttcgggt agcggccgat ggaccgaggc
      241 ggtaaagccc gcgaggcgca gcaactgcgc gaaggccgcc gtgccaagcc cggtcgaaat
      301 cgccagcgcc ggatcgatca cgaagccctt gcccttcgcg gcaacgcgcg cttcgtgcgc
      361 cgcgcgcaag agcttttcgg cgatgtcgag ccggatcgcc tgccggccaa ggctgcgata
      421 gcccggcggc gtggccgagc gcgcggccag ggcaatgacc ggcggcatgc cgggtaccgg
      481 tgcggccact gcccgcccgc ttgccgccag cagggttcgc caggccgcaa gcgcggccgc
      541 cttgagcatt gcgggcatga acagatcgag cgcaccgatc ctgacgccaa gccgccgcat
      601 gaaaccgcgc tgcgtctcgt tgagccgttc aagccctgat ccggcgcgtt cgagcgtgcc
      661 gccggcttcg accaggcgga tcagcagcgc gcgcagttcc ggcccggcct gttcctggcg
      721 cgaggccgct tcgagcgcgg taagcggggc cagggccgcc agtgcatgcc ccaaccactg
      781 ctcgaggccg gcctgcagcg acgggcgggc gagtgcgggc aaggcgccca gcgcggcatc
      841 cagctcgatc cgggggctga gcaggccgcg acccttggcg agctgtgcca ccacttcgcc
      901 ttcccagcag atccgcgcgc catcgaggcc gagatccggc gatgctgcgg ccagggcttc
      961 acccagccga gcggcgcgtt cgcacagcaa tgcgggcagg tgccgttccg ccgccgccag
     1021 cagcagcttg cggtcggcta gccgcgcctg gggatcgatc cgcagccgaa agcctgccag
     1081 cacgccaatg tgctcgccat cgaccagcac ttcgtcatcg accagtctga ccggcagcag
     1141 gctggcatcg ctgccaagct tcttcatcag caccgcagtg cggcgattga cgaagcgttc
     1201 ggtcagccgt gcgtgcaggg catcggagag gcgcgcttcg gcggcgcggg cccgcgctgc
     1261 catttcctcg cgcgccagca cccagtcggg gcgctgggcg atataggccc agctgcgcac
     1321 cgaggcgatc cgtccctgca gcgtgtcgat atctccggcc ggattatcga gatcggcgat
     1381 cgcccgggcg acaaaatcct ggccaagctg gccgcggcgc agatcctgcc acagccgggc
     1441 cacgaagcgc gaatgggttt cggcgccgtg ctggcggaaa tcgggcagcg agcagacctc
     1501 gcggaaccgg cgcaccgccg cctcgccgcg caggcttccg gcgatttcgg gatcttcggc
     1561 caggcgcttg atcaccgcca gatcgatcgc ttcgggggcg ggggccagtt cgggccggtc
     1621 gggcgcgcgt tcgaggtcgg cgatcaggct gcgcaggctg gtgcaatcgg gttcggcctc
     1681 gcgccagaac agccgcgtca gcggggcgaa gcggtgttcc tcgattgcgt agatttcctc
     1741 gtcgctgaac tcggcctcgc gcccgcccgg accggccagc gtgccgaagc tgccgtcctt
     1801 ctggtggcgc ccggcgcgcc cggcgatctg ggccatttcg gccggggtca gccggcgctg
     1861 gcgctgcccg tcgaacttcg acagaccggc gaaggcaaca tgttcgatat cgaggttgag
     1921 ccccatgccg atcgcgtcgg tcgccaccag atagtcgact tcgcccgcct ggtagagctc
     1981 gacctggcgg ttgcgggttt cgggcgagag cgcgcccatc accaccgccg cgccgccgcg
     2041 gaagcgtcgc agcatctcgg ccatggcata gacctgctcg gccgagaagg cgacgatcgc
     2101 gctgcgcggt ggcacccggc tgagcttttt cgccccgaca tggctgagcg tagaaaagcg
     2161 cggacgggtg acgatctcgg cctgtggcac gagcgccttg accagcggct cgagcgtggc
     2221 cgaccccagg atcatcgtct cgtcccggcc gcgcgcgtgc agcagtcggt cggtgaagat
     2281 gtggccgcgt tcgcgatcgg cgcccagctg ggcctcgtcg atccccacga acgaaaggtc
     2341 ggcgcccact ggcatggcct cggcggtgca cagcagccag cgcgcgtgcc tgggttcgat
     2401 ccgttcctcg ccggtgatca gtgcaacccg gtccacaccc ttgattttgc agaccttgtc
     2461 gtagacttcg cgggccagga gccgcagcgg aaagccgatc gcgcccgatg aatgggcgca
     2521 gagccgctcg atcgcgaggt gggtcttgcc ggtattggtg gggcccagca ccgccttgat
     2581 acggctgtcc gaatgagcgc ttgcacggcg ggtcatggta aggatcaatg tggcgtctcg
     2641 ccgccgcgat gcaaggcagg cccgttcttc gtccccagcc aggcgcgcgc acgctgattt
     2701 tccagcccgt cttaggcgaa gattaaccac ctttcgggca aggatttggg caggctgcag
     2761 ctgccgggga cgtgttagtg acaaagcggg gcgaacaggc cggttgggcc gcgggaacgg
     2821 gcatggcacg ggccatggcg atcccggcgg gcgcgatcgt gctggcaccg acccgggccg
     2881 aacgcctgat gcggctgcgc gaagttctgg cgcagcgttt cgcccatctg gaaatcagct
     2941 atgacctggc cgaggacatc ggcagcgcgc cctggttccg cgggctgggc ctgatgctgg
     3001 gcctgggcct gtttgccagc ctgttctggc acgattcggg cgcgattgcc gcgccgccgg
     3061 gcatggtgct cgaccaggac cagcgcgacg aattccgcag ccagatgatc atgccgctgg
     3121 cgctgggcgc cgatagcggg cgacggatgg gggcaaccag cgcgctggtc gcgctggccg
     3181 ccgcgcccga acgccccacg ctgcagttgg tggccaccct gggtcagggc gacagcctga
     3241 gccggatgct ggttcgcgcc ggggtcgaca gcggcgatgc cctgaccgcc gccggactgg
     3301 tggcgatggt catgcccaag ggcgaggttg cccctggcac gcaggccgac ctgacgctgg
     3361 gcaagatcga tccggccacc ggcacccgcc cgcttgaacg catggcgatg cgcgcgcgct
     3421 ttgatcttga tcttgcgctt acccgcaccc cttccgggca gctgacgatc caaccccatg
     3481 cgattgcggt cgacaccacc ccgctgcgca ttcgcggcac cgtgggcacc tcgatctacc
     3541 gctcggcgcg ggccgccggg gcgccgatcg aggcgatcca gcaatacctc caggtgctcg
     3601 accagcatct cagcctcgac acccagatcg ccaacggcga cacctttgac ctgatcgttt
     3661 catacaagcg ttcggcccgc ggcgaggcgc aggttggcga actgctgttc gccgggctcg
     3721 agcgcgatgg caagccgcag gcgcaactgt tgcgctgggg caaggatggg cagttcttcg
     3781 aagcttccgg catgggcgcc cagcgcaccg gcctgatcat gccagtggtt ggccggatca
     3841 cctcgaactt cggggcgcgg cgccatccgg tgctgggcta tacccggatg cacgccgggg
     3901 tcgatttcgg cgccgcgcac ggatcgccga tctacgcggt cggcgatgcc cgggtaatct
     3961 attcgggctg gcacggcggt catggcaact acgtcaagct tgaccatggc gggggctttg
     4021 ccacggccta tgcgcacatg agccggatcg cggtttcctc gggttcggtg gtccgcaccg
     4081 gccagatcat cggctatgtc ggctccactg gcctttcgac cgggccgcac ctgcattacg
     4141 agctttaccg caatggtcag gcggtcaatc cgctgtcggt gcgcttcacc gtctccagcc
     4201 aggtcgatcc ggccgagctc aaggcctaca aggcgcggat ggcggccctt aaagcggtca
     4261 agcccggcgc ggcgatgaac agcctggccc cgcgccaggc cagcgtggcg ggcattggcc
     4321 cccagcgcga gattgatcgc ctcgccaatt gatccagtgc cgattgatga accggcaatc
     4381 ccggcctaaa cagccgggca tgaccggaat gttccccgcc acccgcctgc gccgc
//
LOCUS       DASUDV010000297         4901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_473664,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000297 DASUDV010000000
VERSION     DASUDV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473664"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFV30_06485"
     CDS             complement(<1..434)
                     /locus_tag="VFV30_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847772.1"
                     /translation="MPRTEDAYDFIIVGAGSAGCVLADRLSADGTNKVLLLEAGGSDR
                     SIFIQMPAALAYPMNTKRWNWGYYSEPEPHLNGRRLHCPRGLGLGGSSSINGLVYVRG
                     NPLDFERWQDEDGARGWGYADVLPYFKRAETRAEGGNEYRGG"
     gene            complement(421..2442)
                     /gene="betB"
                     /locus_tag="VFV30_06490"
     CDS             complement(421..2442)
                     /gene="betB"
                     /locus_tag="VFV30_06490"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HEX4847773.1"
                     /translation="MRRREPEDRRIQLLHATMATLAETGVGDLTLAEVARRADVSPAL
                     IVHYFGDREQLLENAFRLLVERATTAPMQALASTHDAEARLIGFIRAHLCATESTSEA
                     SRAWLSLWGLALYQPALARLQRVHQERMASNLRHELRSLVVADQVERIGETIAALIDG
                     FWLRAALARAWNPDAELQVDWILLEVSRMLADARLPEAPAETGTPAKTFDTLNPATGE
                     VLATIKIDGQAEVDKAVARAAEAQKAWGRTPGAERGRILNRAAQLLIERNEGLARLET
                     LDTGKPIQETIAVDVLSGADCIEYFAGVARTIAGEHHDFGPGAFGYTRREPLGVVAGI
                     GAWNYPIQIACWKSAPALACGNAMLFKPAELTPLTAIELEKIYREAGLPEGLFQVLQG
                     IGDTGRLLSRHPGIAKVSLTGEVGTGKKVMADAAPTLKHVTLELGGKSPLIVFDDADL
                     DNAVSGALLANFYSAGEVCSNGTRVFVHRGVMKPFLAKLKARTEAMSVGDPLDPATQV
                     GALISRGHLEKVLGYVARGQAEGATLLTGGERLTEGLLGKGNFMRPAIFAGCHDDMAI
                     VREEIFGPVMAVLEFETEEEVLARANDTQFGLSAGVFTNDLTRAHRVVAGLEAGTCWI
                     NTYNITPIELPFGGNKQSGLGRENGLVAIEHFTQLKSTYVAMEPIDAPY"
     gene            2793..>4901
                     /locus_tag="VFV30_06495"
     CDS             2793..>4901
                     /locus_tag="VFV30_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847774.1"
                     /translation="MTNRSFATRCGQAMRLGTALGLVAAALVATPALAQDAAEEADAG
                     DIVVTAQKREQKVRDVGIAISVLGEEDVQNMGITSATDVVRAVPNLKFNAYGSSQVVF
                     NIRGVSQNDYGDQQEPPVAVYQDDSYASSITTASFPVFDTARVEAVRGPQGTLFGRNA
                     TGGAVQFISNKPTDTAEGYLSATYGRYNQTIIEGAVSGPLGEGVSVRVSGIYNRDDGY
                     IQNITPGEKDRGANNHWALRGIMVIEPSTNFKATVTLRYAQADKERQAGIYNYAPTCP
                     NAQFQGEFMSPTDTCGYWGTTGTAGPGYFNPSIAASQGGDPWATAGTGPSYVDRKIFG
                     ATLRLDATIGALDVVSITDYQHFDKFYIEQGDGQPEIPYVENVTPPATAPCPAPASSV
                     TCYASGTVFYQDANVNQASQELRFSLSSDRNFLTFGAFGMIIDGDYEAKYAVPFIAYD
                     PRVSFSQRTESFALFAQDEFKLSDKVKLIAGVRYWRDNKEGNYSAVHTLAPWALSLNF
                     GPGGVSYTDLTGTNTPASWSFGSGPSGVTITPADASPSFDGITARAEIDYKPSENVLV
                     YASYNRGSKSGGFTFSTGTPFPGALVDTLNNLGYDPETLNAYEVGVKATLTPGTTLNV
                     TGFHYDYKNYQAFVQVGFTQVVRNLPARNTGVEVEFATKPIDGLTIQLSGSWQDSEVK
                     GVLLPDGVTLVNNDLPQTPGF"
BASE COUNT          826 a         1737 c         1531 g          807 t
ORIGIN      
        1 tccccgccgc ggtattcgtt gccgccctcg gcccgggttt cggcccgctt gaaatagggc
       61 aggacatcgg catagcccca accgcgcgcg ccgtcctcgt cctgccagcg ctcgaaatcg
      121 agcgggttgc cgcgcacgta aaccagcccg ttgatcgagc tcgatccgcc cagccccagt
      181 ccgcgcgggc aatgcaaccg ccgaccattg aggtggggtt ccggctcgct gtaatagccc
      241 cagttccacc gcttggtgtt catcggatag gccagcgccg ccggcatctg gatgaagatc
      301 gaacggtccg atccgcccgc ctccagcagc aggaccttgt tggtcccgtc cgcgctcagc
      361 cggtcggcca gaacgcagcc ggccgatccg gcgccaacga tgatgaaatc gtaggcgtcc
      421 tcagtacggg gcatcgatcg gctccatcgc cacataggtg ctcttgagct gggtgaagtg
      481 ctcgatcgcg accaggccgt tctcgcgccc cagccccgat tgcttgttgc cgccgaacgg
      541 cagctcgatc ggggtgatgt tgtaggtgtt gatccagcag gtcccggcct ccagccccgc
      601 caccacgcgg tgcgcgcggg tcaggtcgtt ggtgaagacc ccggccgaga gcccgaactg
      661 ggtgtcgttg gcgcgggcca gcacttcctc ttcggtttcg aattccagca ccgccatgac
      721 cgggccgaag atttcctcgc gcacgatcgc catgtcgtcg tggcaaccgg caaagatcgc
      781 cgggcgcatg aagttgccct tgcccagcag tccctcggtc agccgctcgc ccccggtcag
      841 cagggtggcg ccttcggcct ggccgcgcgc gacatagccc agcacctttt cgaggtgccc
      901 gcgcgagatc agcgcgccga cctgggtcgc gggatccagc ggatcgccca cgctcatcgc
      961 ctcggtccgc gccttcagct tggccaggaa cggcttcatc accccgcggt gcacgaagac
     1021 ccgggtcccg ttcgaacaga cctcgcccgc cgaatagaag ttcgccagca gcgcgcccga
     1081 caccgcgttg tcgagatcgg catcgtcgaa cacgatcagc ggcgatttgc cgcccagttc
     1141 cagcgtgacg tgcttcagcg tcggggcggc atcggccatc accttcttgc cggtgccaac
     1201 ctcgccggtc agcgagacct tggcgatccc cggatggcgg ctcagcagcc ggccggtatc
     1261 gcctatgccc tgcaacacct ggaacagccc ctcgggcagc ccggcttcgc ggtagatctt
     1321 ttccagctcg atcgcggtca gcggggtcag ctcggccggc ttgaacagca tcgcgttgcc
     1381 gcaggccagc gccggggcgc ttttccagca ggcgatctgg atcggatagt tccaggcccc
     1441 gatcccggcg accacgccca gcggctcgcg ccgggtatag ccaaaggcgc cggggccgaa
     1501 atcgtggtgt tccccggcaa tcgtccgggc caccccggcg aagtactcga tgcagtccgc
     1561 gcccgacagc acgtcgaccg cgatagtttc ctgaatcggc ttgccggtat cgagcgtttc
     1621 gagccgggcc agaccttcgt tgcgctcgat cagcagttgc gcggcgcggt tcaggatccg
     1681 gccgcgttcg gcgcccgggg tccgccccca ggccttctgc gcttcggccg cgcgggctac
     1741 ggccttgtcc acttcggcct ggccgtcgat cttgatcgtg gccagcactt cgccggtggc
     1801 cgggttcagg gtgtcaaagg ttttcgccgg cgtgccggtt tcggccggtg cctcgggcag
     1861 gcgggcgtcg gccagcatcc gcgacacttc cagcagaatc cagtcgacct gcagttccgc
     1921 gtccgggttc caggcccggg ccagcgcggc gcgcagccag aacccgtcga tcagcgccgc
     1981 gatcgtctct ccgatgcgct ccacctgatc ggcgacgacc aggctgcgca gctcgtgccg
     2041 caggttcgat gccatccgtt cctggtgcac ccgctgcagc cgggccagcg ccggctggta
     2101 gagcgccagt ccccacagcg agagccaggc gcgcgaggct tccgaggtcg attcggtggc
     2161 gcaaaggtgc gcccggatga acccgatcag ccgcgcctcg gcatcgtggg tagaggccag
     2221 agcctgcatc ggcgcggtcg tcgcgcgctc caccagcagg cggaaggcgt tttccagcag
     2281 ctgctcgcga tcgccgaaat agtgcacgat cagcgccggc gagacatcgg cccggcgcgc
     2341 cacttcggcc agagtcaggt cgcccacccc ggtttccgcc agggtcgcca tggtggcgtg
     2401 aagcagctga attcgccggt cttcgggttc gcgtctgcgc atgagtctgg ccttgtccga
     2461 tcggttgaat attcgttcaa taatccaatc cacagccctt ggcgatgcct gttttcaagg
     2521 cggcggggcg cgattaggcg ttttgatgat tgagttcagc ccgcttgcac aagcgccaat
     2581 cccccgcgat tccggcccgc ttgaccagcc gcaaagcccc tcattccggc gcgatcacgg
     2641 caaaatggcc acagcgggcc ggcaatcgac cggcaatccg gccccggggt tgcacgcgca
     2701 ttcaatatga gtcacctttc gccccggagt ttaggtcgga ccgcgccagt gccgcatgag
     2761 ggcggcgggc gcggaacaag agggagcacg ccatgaccaa ccgttcattc gccacccgct
     2821 gcggacaggc catgcgcctc ggcacggcgc tgggccttgt cgccgccgcg ctggtcgcca
     2881 ccccggccct tgcccaggat gctgcggaag aggccgatgc cggcgatatc gtcgtcaccg
     2941 cccagaagcg cgagcagaag gtccgcgatg tcggcatcgc gatctcggtg ctcggtgaag
     3001 aggacgtcca gaacatgggc atcaccagcg cgaccgacgt ggtccgcgca gtccccaacc
     3061 tgaagttcaa cgcctatggc tccagccagg tggtcttcaa catccgcggc gtttcgcaga
     3121 acgactatgg cgaccagcag gaaccgccgg tcgcggtcta tcaggatgac agctacgcca
     3181 gctcgatcac cacggccagc ttcccggtgt tcgacacggc ccgggtcgaa gccgtgcgcg
     3241 ggccgcaagg caccctgttc ggccgcaacg ccaccggcgg cgcggtgcag ttcatttcga
     3301 acaagccgac cgacaccgcc gaaggctatc tcagcgccac ctacggccgc tacaaccaga
     3361 cgatcatcga aggcgcggtc tccggcccgc tgggcgaagg cgtttcggtc cgcgtgtcgg
     3421 gcatctacaa ccgcgacgac ggctatatcc agaacatcac cccgggtgag aaggaccgcg
     3481 gcgcgaacaa ccactgggcc ctgcgcggga tcatggtgat cgagccttcg accaacttca
     3541 aggccaccgt caccctgcgc tatgctcagg ccgacaagga acgccaggcc gggatctaca
     3601 actacgcgcc gacctgcccc aacgcgcagt tccagggcga attcatgtcg ccgaccgaca
     3661 cctgcggcta ctggggcacc accggcacgg ccggtccggg ctatttcaac ccgtcgatcg
     3721 cggcctcgca gggcggcgat ccctgggcca ccgccggcac cggcccgtcc tatgtcgatc
     3781 gcaagatctt cggcgcgacg ctgcgcctcg atgccacgat cggcgcgctc gatgtggtct
     3841 cgatcaccga ctaccagcac ttcgacaagt tctacatcga acagggcgat ggccagccgg
     3901 aaatccccta tgtcgagaac gtgaccccgc cggccaccgc gccctgcccg gccccggcct
     3961 ccagcgtcac ctgctatgcc tcgggcacgg tgttctacca ggacgccaac gtgaaccagg
     4021 cctcgcagga actgcgcttc tcgctcagca gcgaccgcaa cttcctgacc ttcggcgcct
     4081 tcgggatgat catcgatggc gattacgagg ccaagtatgc ggtgcccttc atcgcctatg
     4141 atccgcgggt cagcttcagc cagcggacgg aaagcttcgc cctgttcgcg caggacgaat
     4201 tcaagctcag cgacaaggtc aagctgatcg ccggtgtgcg ttactggcgg gacaacaagg
     4261 agggcaacta cagcgccgtc cacaccctcg ccccctgggc gctgtcgctc aacttcggcc
     4321 cgggcggggt gtcctacacc gacctgaccg gcaccaacac gcccgcctcg tggagctttg
     4381 gcagcgggcc ttcgggcgtc acgatcaccc cggccgatgc ctcgcccagc tttgacggga
     4441 tcaccgcccg ggcggaaatc gactacaagc cgagcgagaa cgtgctggtc tatgccagct
     4501 acaaccgcgg gtcgaagtcg ggcggcttca ccttctcgac cggtacgccg ttccccggcg
     4561 cgctggtcga tacgctgaac aacctgggct acgatccgga aaccctgaac gcctacgaag
     4621 tgggcgtgaa ggccacgctg acgccgggca cgacgctcaa cgtcaccggc ttccactatg
     4681 actacaagaa ctaccaggcc ttcgtgcagg tcggcttcac ccaggtggtg cgcaacctgc
     4741 cggcccgcaa caccggcgtg gaagtggaat tcgccaccaa gccgatcgac gggctgacga
     4801 tccagctgag cggttcgtgg caggacagcg aggtcaaggg cgtgctcctg cccgacggcg
     4861 tcaccctggt gaacaacgat ctgccgcaga ccccgggctt c
//
LOCUS       DASUDV010000298         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_363274,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000298 DASUDV010000000
VERSION     DASUDV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363274"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1186
                     /gene="purM"
                     /locus_tag="VFV30_06500"
     CDS             83..1186
                     /gene="purM"
                     /locus_tag="VFV30_06500"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX4847775.1"
                     /translation="MANNPPPKSYSYEQAGVSIAAGNALVKAIGPLARATARPGADAE
                     LGGFGGFFDLKAAGYKDPLLVAGNDGVGTKVKLAIDHDRHDAIGIDLVAMCVNDLIVQ
                     GAEPLFFLDYFATGKLDNGVAERVVAGIAEGCKISGCALIGGETAEMPGMYAAGDYDL
                     AGFCVGAVERGEQLTGDRVAAGDVLLGLASSGVHSNGYSLVRRLAADLGWKMDRPALF
                     DPERLLIDTLIEPTRIYVKSLLPLVRSGRIHALAHITGGGLLENVPRVLPAHLHARID
                     AGAWAQPRLLAFLQAQGHIEPAEMARTFNCGIGMVLAVSADEVTGVQAELEAAGETVH
                     VIGAIEPGQRGCTVIGAGEVWSAREPWEAVHLA"
     gene            1230..>2011
                     /gene="purN"
                     /locus_tag="VFV30_06505"
     CDS             1230..>2011
                     /gene="purN"
                     /locus_tag="VFV30_06505"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX4847776.1"
                     /translation="MAALLYAAKAADCPYEIVLVASNNPDAGGLKLAAAEGVQTFVLP
                     HKGMPREEHDGAMDRAIRGSGARYVALAGYMRILTHGFVAGWEGRMLNIHPSLLPSYK
                     GLHTHERAIAAGDSHAGCTVHLVTAQLDDGPILGQTRVAILPGDTADSLAARVLIAEH
                     QLYSRCLAALVSRETSPDWLAGQVRQRALALPETDEVQSHGMPCFGIVKGKKFAYVSS
                     DHHGDGKVALLVKISGADEQAQLIENDPERYYRPAYFGDGWIG"
BASE COUNT          348 a          643 c          680 g          340 t
ORIGIN      
        1 cagtagccat cggggaaaac cgtttgcctt ttgcccaatc atccgtggaa atccaagccg
       61 gaaagcgtta ggcggcgaag cgatggcgaa caacccaccc cccaagagct attcctatga
      121 acaggccggc gtctcgattg ccgctggcaa cgcgctggtc aaggcgatcg gcccgctggc
      181 ccgcgccacc gcgcggcccg gcgccgatgc cgaactgggt ggctttggcg gcttcttcga
      241 tctgaaggcg gccggctaca aggatccctt gctggttgcc ggcaacgacg gggttggcac
      301 caaggtgaag ttggcgatcg atcacgaccg gcacgatgca atcgggatcg atctggtggc
      361 gatgtgcgtc aacgatctga tcgtccaggg cgccgaaccg ctgttcttcc ttgactattt
      421 tgccaccggc aagctcgaca atggcgtggc cgaacgggtg gtggccggga tcgccgaagg
      481 ctgcaagatt tcgggctgcg ccctgatcgg cggcgaaacc gcagaaatgc cggggatgta
      541 cgccgcgggc gattacgacc tggcgggatt ctgtgtcggc gcggtcgaac gcggcgaaca
      601 gctgaccggt gaccgggtgg ccgccggcga cgtacttttg ggcctggcca gttcgggcgt
      661 tcattccaac ggctattcgc tggtccgccg gctcgccgcc gatcttggct ggaagatgga
      721 tcgccctgcc ctgttcgatc ccgagcgcct gctgatcgat acgctgatcg aacccacccg
      781 gatctatgtg aaaagcctgc tgccgctggt ccgttcgggc cggatccatg ccctggccca
      841 tattaccggt ggcggactgc tggaaaacgt gccgcgcgtg ctgcccgcgc acctccacgc
      901 ccggatcgat gccggcgcct gggcccagcc gcggctgctg gccttccttc aggcccaggg
      961 ccacatcgaa ccggcggaaa tggcccgcac cttcaactgc gggatcggca tggtcctggc
     1021 ggtcagcgcc gatgaagtga cgggcgttca ggccgagctt gaagcggcgg gcgaaacggt
     1081 tcacgtgatc ggtgcgatcg agccggggca gcgcggctgc accgtgatcg gcgctggcga
     1141 ggtgtggagc gcgcgcgaac cctgggaagc ggtgcatctt gcctgatcgc gccaaggtag
     1201 ccgtgctcat atcaggcagc ggcaccaata tggcggccct gctctatgcc gcaaaggcgg
     1261 ccgattgccc ctatgagatc gtgctggttg ccagcaacaa tcccgatgcc ggcgggctga
     1321 aactggcggc ggccgaaggg gtgcaaacct tcgtcctgcc gcacaagggc atgccccggg
     1381 aagagcatga cggggcgatg gaccgggcaa tccggggcag tggcgcgcgc tatgtcgcgc
     1441 tggccgggta tatgcggatc cttacccacg gcttcgttgc cggctgggag gggcggatgc
     1501 tcaacattca ccccagcctg ctgcccagct acaagggcct gcacacccac gagcgcgcga
     1561 tcgcagcggg cgacagccat gccggctgca ccgtgcacct tgtcaccgcg cagctcgacg
     1621 atggcccgat cctgggccag acccgggtgg cgatcctgcc aggcgatacg gcggacagcc
     1681 ttgccgcgcg ggtgctgatc gccgaacacc agctctattc gcgctgcctt gcagcactgg
     1741 tgagccgcga gacttcgccc gactggctgg ccgggcaagt acgccaacgc gcgctggccc
     1801 tgcccgaaac cgacgaggtg cagagccacg gcatgccctg cttcgggatc gtgaagggca
     1861 agaagttcgc ctatgtttca agcgatcacc acggcgatgg caaggtcgcg ctgctggtca
     1921 agatcagcgg cgctgacgag caggcccagc tgatcgagaa cgatcccgaa cgctattatc
     1981 gcccggccta tttcggcgat ggctggatcg g
//
LOCUS       DASUDV010000299         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_716456,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000299 DASUDV010000000
VERSION     DASUDV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716456"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFV30_06510"
     CDS             complement(<1..429)
                     /locus_tag="VFV30_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847777.1"
                     /translation="MAVKSNLTWRRSSILALAVAALTLPGAAQADPDRGRAWGREARS
                     EAPPPPPPAPAPQQQRSWSPPPQRQAPQAAPPQAAPQQAAPQQRWGGNRESGWSGGNR
                     GGRQGNAEAPPVQQQPAQGSWNNRGGNDDRREWRGERRDRG"
     gene            555..1148
                     /locus_tag="VFV30_06515"
     CDS             555..1148
                     /locus_tag="VFV30_06515"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX4847778.1"
                     /translation="MSDGIETLEAVLADCTARLQQAARDRRSAMHVPVVGTADGDLRM
                     MVLRACAPDLALLRLHTDARSPKARTIGAGGRVSLLAFDGAAKIQLRLRGAGRIEQRG
                     PVADAAWAEATAFARRCYLAEAAPGTAAERPLSGLPEDVEGTMPSEAQLIPARENFAV
                     LLIEPDSLDWLYLANSGHRRAQFTRGDGGWQGTWVVP"
     gene            complement(1153..>2299)
                     /gene="ettA"
                     /locus_tag="VFV30_06520"
     CDS             complement(1153..>2299)
                     /gene="ettA"
                     /locus_tag="VFV30_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX4847779.1"
                     /translation="RLLIQKPDILLLDEPTNHLDAESVEWLENHLKEYAGAVLMITHD
                     RYFLDNVVGWILELDRGKYFPYEGNYSTYLEKKAKRLEQESREESGKQKALQRELEWI
                     RQTPAARQTKSKARIRKFEELQNAQDNRPVGKAQIVIQVPERLGGKVIEVKNISKAYG
                     DKLLFENLSFMLPPGGIVGIIGPNGAGKSTLFRILTGKEEPDTGTVEIGSTVHLGYVD
                     QSRDDLNPDNNVWQEISGGHDYMTVNKQEMSTRAYVGAFNFKGADQQKNVGKLSGGER
                     NRVHMAKMLKQGGNVLLLDEPTNDLDVETLAALEDAIENFAGCAVVISHDRFFLDRLA
                     THILAFEGNSHVEWFEGNFAMYEEDKRRRLGDAADRPTRLAYKKLTR"
BASE COUNT          304 a          747 c          806 g          442 t
ORIGIN      
        1 accgcgatcg cggcgttcgc cgcgccattc gcgccggtca tcgttgccgc cgcggttgtt
       61 ccagctgccc tgggcgggct gctgctgcac cggcggcgcc tcggcattgc cctggcgtcc
      121 gccgcggttg ccgcccgacc acccgctctc acggttgccg ccccagcgct gctgcggcgc
      181 ggcctgctgc ggagccgcct gcgggggtgc ggcctgcgga gcctggcgct gcggcggggg
      241 cgaccagctg cgttgctgct gcggagccgg agccgggggc ggaggcggcg gggcttcgct
      301 gcgggcttca cgcccccagg cccggccgcg atccggatcg gcctgggccg caccgggcag
      361 cgtcagcgcc gcgacggcca gggcaagaat gctggagcga cgccaggtaa ggtttgactt
      421 cacggccatg gccgcttctc catctagaaa gcaccgcccg attgcggcga cattgaggca
      481 tgggctacgc gagtcgtgct gtcggcaggg tgaatgactt ggtaaggttt gagttcagct
      541 ttgtggggct ttgaatgagt gacggaatcg agacgctgga ggcggtgctg gccgattgca
      601 ctgcgcggct gcagcaggcc gcgcgcgacc ggcgcagcgc gatgcatgtg ccggtggtcg
      661 gcaccgccga tggcgatctt cgcatgatgg tcttgcgcgc ctgcgctccg gacctggcgc
      721 tgctgcgcct ccacaccgac gcccgcagcc ccaaggcccg cacgatcggt gctggcggcc
      781 gggtcagcct gctggctttc gacggggcgg ccaagatcca gctgcggctg cgcggggcgg
      841 ggcggatcga gcagcgcggg cccgtggccg acgccgcctg ggccgaagcc accgcctttg
      901 cccgccgctg ctatctggcc gaggccgcgc ccggcaccgc ggccgagcgg ccgctgtccg
      961 gcctgccgga agatgtcgaa gggacgatgc ccagcgaagc ccagctgatc cccgcgcggg
     1021 agaatttcgc ggttctgctg atcgagcccg attcgctcga ctggctgtac ctcgccaaca
     1081 gcggccaccg ccgcgcgcag ttcacgcgcg gggatggggg ctggcagggg acctgggtgg
     1141 tcccctgacc ggtcagcgcg tcagcttctt gtaggcaagc cgggtcggac gatccgccgc
     1201 gtcgcccagg cggcggcgct tgtcttcctc gtacatcgcg aagttgcctt cgaaccattc
     1261 gacgtggctg ttgccttcga aggcgaggat gtgggttgcc aggcgatcga ggaagaagcg
     1321 gtcgtgcgag atgaccacgg cgcagccggc gaagttctcg atcgcgtctt ccagcgcggc
     1381 gagggtttcg acgtcgaggt cgttggtcgg ttcgtcgagc agcagcacgt tgccgccctg
     1441 cttcaacatc ttggccatgt ggacgcggtt gcgttcaccg cccgagagct tgccgacgtt
     1501 cttctgctgg tccgcgccct tgaaattgaa cgcgccgaca taggcccgcg tgctcatttc
     1561 ctgcttgttg acggtcatgt aatcgtgccc gccagagatt tcctgccaca cgttgttgtc
     1621 cgggttcagg tcgtcgcggc tctggtcgac atagcccagg tgaaccgtgc tgccgatttc
     1681 gaccgtgccg gtatcgggct cttccttgcc ggtcaggatc cggaacaggg tggacttgcc
     1741 cgcgccgttc ggcccgatga tcccgacgat gccgcccggg ggcagcatga acgagaggtt
     1801 ttcgaacagc agcttgtcgc cataggcctt cgagatgttc ttcacctcga tcaccttgcc
     1861 gcccaggcgc tcgggcacct ggatgacgat ctgggccttg ccgaccgggc ggttgtcctg
     1921 cgcgttctgc agttcctcga acttgcggat acgggccttg gacttggtct ggcgggccgc
     1981 cggggtctgc cggatccact ccagctcgcg ctggagcgcc ttctgcttgc ccgattcctc
     2041 gcggctttcc tgttcaaggc gcttggcctt cttttccagg taggtcgaat agttgccctc
     2101 gtaggggaag tactttccgc ggtcgagttc gaggatccag cccaccacgt tgtcgaggaa
     2161 atagcggtcg tgggtgatca tcagcaccgc gccggcatat tccttgaggt ggttttccag
     2221 ccattcgaca ctctcggcat cgaggtggtt ggtcggttcg tcgagcagca ggatgtccgg
     2281 cttctggatc agcaggcgg
//
LOCUS       DASUDV010000300         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1003606,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000300 DASUDV010000000
VERSION     DASUDV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003606"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFV30_06525"
     CDS             complement(<1..1395)
                     /locus_tag="VFV30_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX4847780.1"
                     /translation="MAAADGAPVPAAAPQVETVGAARVLARPLVAPPPAWVDTPAMPR
                     PPRDAEGAATLDLLGDVQVLFGKESDTFYYGQAWQIGTAQGLDGGSLQIDWDPALEKL
                     TIHRYRVLRDGQPIDLLGDGSRLSVIQREKRMESAMLDGRLTTTYQPEDLRVGDVIDL
                     AYTIERRDPALAGRSQYVVGPKDGATFGRFRARMLWGNDKAMRWRVYPGILQPHLIKT
                     AKGSELVADLGEMSAPLAPEGAPSRFQLVNAIEITEFADWQGVSRSFAPLYAEAIKLA
                     PNSPIRAEAARIAAQTRDPRRRAELALQLVQEQIRYLFMGMNAGGFVPASAEQTWARR
                     FGDCKAKTVLLIALLGELGIEARPVLVHTQQGDLLTARLPNMESFDHVIVEAKIAGKS
                     HWLDGTRLGDLSLDQLETPNWKAGLPVTAQGSALVRMEPDPLDAPREFVSLTLDASRG
                     LDLPASAVGEMRFHG"
     gene            complement(1494..2345)
                     /locus_tag="VFV30_06530"
     CDS             complement(1494..2345)
                     /locus_tag="VFV30_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847781.1"
                     /translation="MEHHTVRVAVADGVEIAADVAGRHGAPTVVLGHGGGQTRHSWDK
                     CELQLAEAGYFAINYDLRGHGDSDWSSDGDYSLETRADDLAAVAALGSRPFALVGASL
                     GGITAMVAASRGLDPAALVLVDIVPKMSPKGVAKITGFMTANPDGFASLEEAADAIAE
                     YYPDRPRPKDNSGLHKNLRLGEDGRYRWHWDPRWLQSPRGDPHFLLEMMDAADWTDRV
                     PTLLVRGMKSDIVDDDGVEDLRRRIPQLEVADIRGAGHMVAGDKNDEFNAAVIEFLAR
                     VMPARQV"
     gene            complement(2403..2567)
                     /locus_tag="VFV30_06535"
     CDS             complement(2403..2567)
                     /locus_tag="VFV30_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX4847782.1"
                     /translation="MRKWKCRNCGFIYDEALGWPEEGIAPGTRFEDIPDDWYCPDCGT
                     EKDDFELVEY"
     gene            complement(2579..>2780)
                     /locus_tag="VFV30_06540"
     CDS             complement(2579..>2780)
                     /locus_tag="VFV30_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4847783.1"
                     /translation="EQNPQIAFGYGVHVCLGQHLARMEMRILWEELFKRLKSVEMNGK
                     GERTISNFVCGPKHVPIRFKAN"
BASE COUNT          486 a          941 c          863 g          490 t
ORIGIN      
        1 gccgtgaaag cgcatttcgc caaccgcgct ggccggcaga tcgagcccgc ggctggcatc
       61 gagcgtgagg ctgacgaatt cgcgcggcgc atcgagcgga tcgggttcca tcctgaccag
      121 cgccgaaccc tgtgcggtaa ccggcaatcc ggccttccag ttgggcgttt caagctgatc
      181 gaggctgaga tcgcccagcc gggtgccatc gagccagtgg ctctttccgg caatcttcgc
      241 ctcgacgatc acgtgatcga agctttccat gttgggtagc cgggcggtca aaagatcacc
      301 ctgctgggtg tgcaccagca ccgggcgcgc ctcgatcccc agttcgccca gaagcgcgat
      361 cagcagcacg gtcttggcct tgcaatcgcc gaaccggcgc gcccaggtct gttcggcgct
      421 ggccggaacg aaaccgcccg cgttcatccc catgaacagg taacggatct gttcctggac
      481 cagttgcagc gccagttcgg cacggcgccg gggatccctg gtctgcgcgg caatccgggc
      541 ggcttcagcc cggatcgggc tgttgggcgc aagctttatg gcttcggcat aaagcggggc
      601 aaagctgcgc gaaacgccct gccagtcggc aaattcggta atctcgatgg cattgaccag
      661 ctgaaagcgc gatggcgcac cttcaggggc cagcggcgcg ctcatttcgc ccaggtcagc
      721 caccagttcg ctgcctttcg cggtcttgat caggtggggc tggagaatgc cgggatagac
      781 ccgccaacgc atcgccttgt cattgcccca cagcatccgg gcgcggaacc ggccgaaggt
      841 ggcgccatcc ttgggcccga ccacgtactg gctgcgcccg gccagcgccg gatcacgccg
      901 ttcgatggta taggcaagat cgatcacgtc gcccacgcgc aggtcttcgg gctgataggt
      961 cgtggtcagc cgtccatcga gcatcgcgct ttccatgcgc ttttcgcgct ggatcaccga
     1021 aagcctcgaa ccatcgccca gcaggtcgat cggctggcca tcgcgcagca cccggtagcg
     1081 gtgaatggtc agcttttcga gcgccgggtc ccagtcgatc tgcaaggatc cgccatcaag
     1141 cccctgcgcg gtgccgatct gccaggcctg gccatagtag aaggtatcgg actcctttcc
     1201 gaacagcacc tggacatcgc ccaacaggtc aagcgttgcg gcgccttcgg cgtccctggg
     1261 cgggcggggc atggcaggcg tatcgaccca ggccggcggc ggcgccacca gcgggcgcgc
     1321 cagcacccgt gccgctccaa ccgtttcaac ctgcggcgcg gctgccggca ccggcgcgcc
     1381 atcggcggca gccatggcca tggatggcat gcccgcaagc agaagggcga aacatttgaa
     1441 ccgcatgacc actccagact gcgcactgtc gcagccaaga gttacgcaat tgcttagact
     1501 tgtcgagccg gcatcacccg cgcgaggaat tcgatcaccg cggcgttgaa ttcatcgttc
     1561 ttgtccccgg caaccatgtg cccggcgccg cgaatatcgg caacctcgag ctgcgggatg
     1621 cggcggcgca ggtcctccac cccgtcatcg tcgacgatat cgctcttcat gccgcgcacc
     1681 agcagggtcg gcacccgatc ggtccagtct gcggcatcca tcatttccag caggaaatgc
     1741 ggatcgccgc gcggtgattg cagccagcgc ggatcccagt gccagcgata acgcccgtcc
     1801 tcgcccagcc gcaggttctt gtgcagcccg gaattgtcct tggggcgggg ccggtcggga
     1861 taatattcgg cgatcgcatc ggcggcctct tcaaggctgg caaagccatc gggattggcg
     1921 gtcatgaaac cggtgatctt ggccacgccc ttggggctca tcttggggac gatatcgacc
     1981 agcaccagcg ccgccggatc aagcccgcgc gaggccgcga ccattgcggt gatcccgccc
     2041 agcgaagcgc cgaccagcgc aaacggccgc gatccaagcg cggccaccgc ggcaagatca
     2101 tcggcccggg tttccaggct gtaatcgcca tcgctcgacc agtcgctatc gccatggccg
     2161 cgcaggtcat agttgatcgc aaagtagccg gcttcggcga gctgcagttc gcacttgtcc
     2221 cacgaatgcc gggtctgccc gccgccgtgc cccagcacca cggttggcgc gccgtggcgc
     2281 ccggcgacat cggcggcgat ttcaacccca tcggcaacgg caacgcggac tgtgtgatgt
     2341 tccatcaggc acagggatgc ccgccgcggc aggcccggtc aaccgctcat ttaatcgagc
     2401 ggttaatatt ccaccagttc gaagtcgtcc ttctcggtcc cgcagtcggg gcaataccag
     2461 tcgtcgggaa tatcctcgaa gcgagtgccc ggcgcgatcc cttcttcggg ccagcccagc
     2521 gcctcgtcat agatgaagcc gcagttgcgg cacttccact tgcgcatcgc cgcccccgtc
     2581 agttggcctt gaagcggatc ggcacgtgct tgggcccgca cacgaagttc gaaatggtgc
     2641 gctcgccctt gccgttcatc tcgaccgact tgaggcgctt gaacagctct tcccacagga
     2701 tccgcatttc catgcgcgcc aggtgctggc caaggcatac gtggacgcca tagccgaagg
     2761 cgatctgcgg gttctgctcg
//
LOCUS       DASUDV010000301         4292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_672384,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000301 DASUDV010000000
VERSION     DASUDV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672384"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFV30_06545"
     CDS             <1..709
                     /locus_tag="VFV30_06545"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX4847784.1"
                     /translation="VPETGSTNADLAARLVGAEGLGEGTWLVADRQTAGRGRQGRTWF
                     DGHGNFMGSTVVRPGPGDPPAASLALLSGLAVHEAVAPLIPPPATALLKWPNDVMVGP
                     AKLAGILLERVGDAVIVGIGVNLAQAPDVKGRQTVALSAFGPAPDRDHFASALARQFD
                     LELERWRAYGLDPLIRRWQAAAHPPGTPLLVGEPGEAALEGRFAGLTGDGALQLRLAD
                     GTTRVIHAGEVRLAGGQ"
     gene            720..1505
                     /locus_tag="VFV30_06550"
     CDS             720..1505
                     /locus_tag="VFV30_06550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX4847785.1"
                     /translation="MLLAIDNGNTNVKFALVNTAGDIVQRWRLATDIKRTADEYAVWL
                     DQLLRMEGYARSDVNAVVIASVVPRALHNLQELAAKYFGVEALVGGRPPVEWGIALKV
                     DEPRALGADRAVNAIAAQALEAGDKIVISFGTATTFDHIGADGAYLGGSIATGVSLSL
                     DALYNAAAMLPRIAIEPPPSASVIGRDTVGQMQIGIYWGYVSMIEGMIARMKAEIGRP
                     VKVIATGGLAPLFQQHGHLFDRVEPDLTLRGIALLYANRAEAK"
     gene            1502..3136
                     /locus_tag="VFV30_06555"
     CDS             1502..3136
                     /locus_tag="VFV30_06555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX4847786.1"
                     /translation="MKPGNELLFCALGGSGEIGMNVNLYGCQGKWLMVDLGMTFGANE
                     YPGTELVFADLEFIEDRKADLLGVVLTHAHEDHIGAVPYFAEELGVPLYATPFTARLV
                     AEKLEEAGLSHSVELRVVDHLDRFDLGPFGIRYLPLAHSIAEGNALVIETPHGRIFHT
                     GDWKLDDDPLVGVPATAEELTQIGDEGVLALVCDSTNVFNPKASGSEGGVRDALMEEV
                     AKHHGRRVLVTTFASNVARLQTLGDVARASNRQLCVAGRSLDRIIRTAQASGYLRDLP
                     DRIDFDTAMGLPRGEVLIVATGGQGEPRAALARIAEGNHPLALEAGDVVLFSSRQIPG
                     NEIAIGRIQNRLSGRDVVLVTDRQSPIHVSGHPGRPELEAVYGWLRPEILLPVHGEVR
                     HMKEQARLGRACGIPKTVVQQNGDMVRLAPGKPGKISEVRAGRLILDGDIIAPADGEA
                     MVMRRRLAANGLVIVVLDAKGRVHVEAIGLPLDEDMDDFVKEAREDVAAALAKLKGGQ
                     RHDREAVSEAARLAARRAAQRWSGKKPQVRVILPEQ"
     gene            3141..3407
                     /locus_tag="VFV30_06560"
     CDS             3141..3407
                     /locus_tag="VFV30_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX4847787.1"
                     /translation="MKLTSILAIYFLFWVMSAMFILPFGQRTHDEAGAELVPGQVSSA
                     PVNFDPKKVAKRATVLSLVLFGLYYLNYVQGWFTVEDIDITNYI"
     gene            complement(3415..>4292)
                     /locus_tag="VFV30_06565"
     CDS             complement(3415..>4292)
                     /locus_tag="VFV30_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX4847788.1"
                     /translation="QALSGHVSEAAGGLTQALTQVEALSTILTAEGTRQAEQVDAMRA
                     SLESVRSESLGLAEQAQAELRKAIDELNASAKAAVSGIAELSAGSISKLAASLGEESG
                     AAIEKAMRSKAAEVAGQLELAASHAAGVSREAAVQLRDQLAKVNELTAHLERRVAHAR
                     SKAEDQVDNDFARRVALITESLNSNAIDIARGLDTDVTDTAWAAYLKGDRGIFTRRAV
                     KLLDSGEAKAVAQLYEQDRTFREHVSRYIHDFEAMLRQLLSTRDGHALGVTILSSDMG
                     KLYVALAQAIERLRN"
BASE COUNT          665 a         1412 c         1497 g          718 t
ORIGIN      
        1 cgtccccgaa accggatcga ccaacgccga tctcgccgcg cgcctggtcg gcgcggaagg
       61 gctgggcgag ggcacctggc tggttgccga ccgccagacc gccgggcgcg gccgccaggg
      121 gcggacctgg tttgacgggc atggcaattt catgggctcc accgtggtcc gccccgggcc
      181 gggcgatccg ccggcggcct cgctcgcgct gctgagcggg ctggcagtgc acgaagcggt
      241 cgcgccgctg atcccgccgc cggcaaccgc gctgctcaaa tggccgaacg acgtgatggt
      301 cggcccggcc aagctggccg gcatcctgct ggaacgggtg ggcgatgcgg tgatcgtcgg
      361 gatcggggtc aacctcgccc aggctccgga tgtcaagggg cggcagacgg tggcgctctc
      421 cgccttcggc cctgcgccgg accgcgatca cttcgcctcc gcgcttgccc gccagttcga
      481 tctggaattg gaacggtggc gggcctatgg cctggatcca ctgatccgcc gctggcaggc
      541 tgcggcccat ccgccaggca ctccgctgct ggtcggggag ccgggcgagg cggcgctgga
      601 agggcgcttt gccggactga ccggcgatgg ggccttgcaa ctgcggcttg ccgacggcac
      661 gacgcgcgta atccacgctg gcgaggttcg ccttgccggc ggccaatagg gacagggcca
      721 tgctgctggc gatcgacaac ggcaatacca atgtgaagtt cgcgctggtc aacacggcgg
      781 gcgatatcgt gcagcgctgg cggcttgcca ccgatatcaa gcgcacggcc gacgaatacg
      841 ccgtctggct ggaccagttg ctgcggatgg agggctatgc ccggtcggac gtgaacgcgg
      901 tggtgatcgc ctcggtcgtg ccgcgcgcgc tgcacaacct gcaggagctg gccgccaagt
      961 acttcggggt agaggcgctg gtcggcgggc ggccgccggt cgaatggggc atcgccctca
     1021 aggttgacga gccgcgcgcg ctcggagcgg accgcgcggt caacgccatt gccgcccagg
     1081 cgctggaggc cggggacaag atcgtgatct cgttcggcac ggccaccacc tttgaccaca
     1141 tcggcgccga cggggcctat ctcggcggct cgatcgccac cggggtctcg ctctcgctcg
     1201 atgcgcttta caacgcggcc gccatgttgc cgcggatcgc gatcgaaccg ccgcccagcg
     1261 ccagcgttat cgggcgtgac acagtcgggc agatgcagat cggcatctat tggggctatg
     1321 tcagcatgat cgaaggaatg attgcccgca tgaaagccga aatcggacgc cccgtgaaag
     1381 tgatcgccac cggcgggctc gcgccgctgt tccagcagca cggccacctg tttgaccggg
     1441 tcgagccgga cctgaccctg cgcggcattg ccctgcttta cgccaaccgg gcagaggcca
     1501 agtgaagccc ggcaacgaac tgctgttctg cgcgctcggc gggtcgggcg agattggcat
     1561 gaacgtcaat ctctatggct gccagggcaa gtggctgatg gtcgatctgg gcatgacctt
     1621 cggcgccaac gagtacccgg ggaccgagtt ggtctttgcc gacctggaat tcatcgagga
     1681 ccgcaaggcg gacctgctgg gcgtggtgct gacccacgcg cacgaggacc acattggcgc
     1741 cgtgccctat ttcgccgaag agctgggcgt gccgctctat gccacgccgt tcaccgcgcg
     1801 gcttgtcgcg gaaaagctgg aagaggccgg gctgagccac agcgtcgaat tgcgggtggt
     1861 ggaccacctc gaccggttcg atctggggcc gttcggcatc cgctatttgc cgctcgccca
     1921 ctcgattgcc gagggcaacg cgctagtaat cgaaaccccg cacggccgca tcttccacac
     1981 cggcgactgg aagctggatg acgatccgct ggtcggcgtg ccggccacgg ccgaggagct
     2041 gacccagatc ggcgacgagg gcgtgctggc gctggtctgc gattccacca atgtgttcaa
     2101 ccctaaggcc tccggttcgg aaggcggggt gcgcgatgcg cttatggagg aagtggccaa
     2161 gcaccatggc cgccgggtgc tggtcaccac ctttgcctcg aacgtcgccc ggctccagac
     2221 cctcggcgat gtcgcccgtg cgtcgaaccg ccagctgtgc gtcgcgggcc gctcgctcga
     2281 ccggatcatc cggaccgccc aggccagcgg ttacctcagg gacctgcccg accggatcga
     2341 tttcgatacc gcgatgggcc tgccgcgcgg cgaagtgctg atcgtggcga ccgggggaca
     2401 gggcgagccg cgagcggccc tggcccggat tgccgagggc aaccacccgc tcgcgctgga
     2461 agcgggcgat gttgtcctgt tttccagccg ccagattccg ggcaacgaga ttgcgatcgg
     2521 gcggatccag aaccgccttt cgggacgcga cgtggtgctg gtgaccgacc ggcagagccc
     2581 gatccacgtt tccggccacc cgggacggcc ggagctcgaa gcggtctatg gctggctgcg
     2641 cccggagatc ctgctgccgg tccacggcga ggtgcggcac atgaaggaac aggcacggct
     2701 tggccgggcc tgcggcattc cgaagaccgt ggtccagcag aatggcgaca tggtgcgcct
     2761 ggctccgggc aagccgggca agatttccga agtccgcgcc gggcgcctga tcctggatgg
     2821 cgatatcatc gcgccggccg atggcgaggc gatggtgatg cgccgccgcc tggccgccaa
     2881 cgggttggtg atcgtcgtcc tcgatgccaa gggccgggtc catgtcgaag cgatcggctt
     2941 gccgctcgat gaggacatgg acgatttcgt gaaggaagcg cgcgaggacg ttgcggcggc
     3001 gctggccaag ctcaagggcg gtcagcggca tgaccgcgag gccgtgtccg aagccgcccg
     3061 cctggctgcc cgccgggcgg cgcagcgctg gtcggggaag aagccgcaag ttcgagtcat
     3121 cctgccggag caatgacccc atgaaactca cctcgatcct ggcgatctac ttcctgttct
     3181 gggtgatgag cgcgatgttc atcctgccgt tcgggcagcg cacccatgac gaggccggtg
     3241 ccgaactggt cccggggcag gtcagcagcg ccccggtcaa cttcgatccg aagaaggtgg
     3301 ccaagcgcgc caccgtgctc tcgctcgtgc tgttcgggct ctactatctc aactacgtgc
     3361 agggctggtt cacggtcgag gatatcgaca tcacgaacta catctaggcg ctgcctagtt
     3421 gcgcaggcgc tcgatcgcct gggccagcgc gacatagagc ttgcccatgt cggacgagag
     3481 gatcgtcacg cccagcgcgt ggccatcccg cgttgacagc agctgccgta gcatcgcctc
     3541 gaaatcgtgg atgtagcggc tcacatgctc gcgaaaggtg cggtcctgtt cgtaaagctg
     3601 ggccacggcc ttggcctcgc cactgtcgag cagcttgacc gcgcggcggg taaagatccc
     3661 gcggtcgccc ttgaggtacg ccgcccaggc cgtgtcggtg acatcggtgt cgagcccgcg
     3721 ggcaatgtcg atcgcgttgg aattgaggct ttcggtgatc agcgccacgc gccgggcaaa
     3781 gtcattgtcg acctgatcct cggccttgga ccgggcatgg gccacgcgcc gctccaggtg
     3841 ggcggtcagt tcgttaacct tggcaagctg atcgcgcagc tgcactgcgg cctcgcggct
     3901 gacaccggcg gcgtgcgagg cggccagctc cagctgaccc gccacctcgg cggccttgga
     3961 gcgcatcgcc ttctcgatcg cggcgccgct ttcctcgccc aggctggcgg caagtttcga
     4021 tatcgagccg gcgctcagtt cggcaatccc gctgacggcc gccttggccg aggcgttcag
     4081 ttcgtcgatc gccttgcgca gttccgcttg cgcctgttcg gccaggccca gactttcgct
     4141 gcggaccgat tccagcgagg cgcgcatcgc gtcgacctgt tcggcctggc gggtcccctc
     4201 ggcggtcagg atcgtgctga gcgcttcgac ctgggtcaga gcctgggtca gaccgccggc
     4261 ggcttcgctg acatggccgg acagcgcctg gc
//
LOCUS       DASUDV010000302        14018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1777039,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000302 DASUDV010000000
VERSION     DASUDV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777039"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFV30_06570"
     CDS             <1..240
                     /locus_tag="VFV30_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4847789.1"
                     /translation="GVNLGVLAQHGSLFVTRMTLFDYYREPAEALAGAAKLWDMVGSG
                     KLTVAIGQTYALTDAAQAHRDLEARQTTGSTLLLP"
     gene            complement(287..904)
                     /locus_tag="VFV30_06575"
     CDS             complement(287..904)
                     /locus_tag="VFV30_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847790.1"
                     /translation="MSGRQQMGPGGWWLVAAMVMGLGAVLWLGVLLAGQIPEYRALRS
                     EGRTVDGVIRSKEVESTTSSRAKRNNTSENRYFVIAFNSRAGVPFGTAPAADAAVTAK
                     PRSGAEIVAGLTIGGSGAAASGPTDTTVRINAGSFERFEAHAIGSAISVTYLPADPKV
                     AKLTDSVRAFNPWLRLGGAIVLALAALGAAWIGWNRRKTHLGRGV"
     gene            complement(924..2036)
                     /locus_tag="VFV30_06580"
     CDS             complement(924..2036)
                     /locus_tag="VFV30_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847791.1"
                     /translation="MRKLAILAEFALIAGLTTSATVAQIPSVGDAIGGAKTPRPARPD
                     RGGKQEQPNSNQGTSFSAPAAPQGASQGITSDLHRQNIGRIVFSGQDMPLDRMNAGML
                     KSAFNLGDEIYFRVYMAEPAPTRLYGRVPGMDDHNTAYFTQYKMLFTVDGRPYEVSQR
                     PWGTAEEYKTWTTWRGQLLSRGERFFPGQEGFGELLSRATRKGDLGPGTYKITLEMIP
                     VVIVNEGTSGKNNFVEKARGAAVAKGEFTLTVRPGAYNRSDPKICGTQAVQANPALEG
                     TILSHARSVWNRPSAPPVGVRITHSGWTIVRHPVTGIILHRQIDAAIVSRGAEFCDIQ
                     GYSYSQPYVGGAFSAGNAKFNGAETYGTYIPCSCIG"
     gene            complement(2050..5385)
                     /locus_tag="VFV30_06585"
     CDS             complement(2050..5385)
                     /locus_tag="VFV30_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX4847792.1"
                     /translation="MSSNLRPKSTRHVRRAALGTAALWCLLDPGQAWASCALTSGSGT
                     VASPNAGSTVTCTNATNGLSINSSADDVTVVVDPNALVQGNPGDTVALTGNRAFMILN
                     SLATVNDLSINLGNSGATGPVMLALNGGTLTNTNAANTIALSGNGVRLTIDGGQLTTA
                     STVSLSGTNARFELSGGGAIVAAPTLNGPLVQGGSSNEQFFLHGRMIGRDSGGTIAYL
                     DAGDGDDTVVISSRTELTELSTTTPLASAISIRGGAGFDTLEGDGGFGDGQIAFSTSD
                     FERLHLYGGSNWQLGGSHAFQDIRVDTGVTLTANTFTALGQATSTLDLRGGVLGAGRL
                     VVGDSGTHQFQHVLLGDGEIEFINTGDYTFASASPSFSGRVINTSAVLRVSHAQAFGL
                     AEVVNNGWLHLNDVTLGNRLSGNGIIVVSGTSSTITNSFNSIGGLVNVTSGDLRIQDL
                     RVLGGGNTASPAIVSIGFGGRLVLDIGGSSFPDPLDPSQIFHNTLNGYGTLVKQGTGA
                     VVISRDNQGFFGQTRIEGGWLHAGHQNALGSGTVTITAGGLSLGDITFGNTLVGSGPV
                     TKDGTGRTTMTGLNQGFTAIWSVNAGTLAGTSSGNFGGSGSVINLSGQATFELTTATN
                     HDLDLRVSGFAGSTFRKLGAGRLTFVDLFTVGTLAVDAGSVRVNRPISANAVVGSAAR
                     LDGTGSITGSLTNNGTVAPGNSIGTLTINGNYTHNAGSVLEIEFDANGNIDLLAVNGS
                     VSLNGGTLRFISVGGAEGNGGTFLTASGGITGTFATVDTVGAQLPLAVIYQPSSALMA
                     PSVLTARPSTFNAQFLAAAETGSGFADLSESAIRRMPRGTRLWLDGFAGDAQRSASGQ
                     TLAYGHDSYGLSGGVTFQLGETTTLGLSAGWARGDIELGAGGGGGKQSSVLGGVTLRH
                     DIGDAKLTGGVLLGRIDQDTVRNVSFNGFSATVQGETESTLYGGFIGLDLPLGRIGKW
                     SLGGTLRGTWIRQDQDGYTESGTSPLRLSLGDFSADTLEGQAGLRIGRHFGPLGGGAD
                     FRVEFGGRVLGVNGDRLIPVSFAASNAGVVLQGDTRDSLHGYLGAALDVAVGRSAQLH
                     IGYSGQFGTSDRHLGRVGFSLGF"
     gene            complement(5475..6122)
                     /locus_tag="VFV30_06590"
     CDS             complement(5475..6122)
                     /locus_tag="VFV30_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4847793.1"
                     /translation="MQTQVAIVEDDPLVRGYLADVIAADGGYALVGTAASLAAARGLL
                     DRGVDVYLLDLGLPDGNGASFIPQVRAAGEAKVIVLTSFGDRDTVVRTIEAGADGYLL
                     KDSATPDILAAIEAALAGGAPISAAAAVHLLERLRSFPAPTPGEDAATPLSPRETELL
                     QLFAKGYSYKEAARILAISPLTVGNHVRSIYRKLDVHTRSEAVYEALRSGKLNLG"
     gene            complement(6172..8091)
                     /locus_tag="VFV30_06595"
     CDS             complement(6172..8091)
                     /locus_tag="VFV30_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX4847794.1"
                     /translation="MGGLADRVAAFWANRAPVDRRSLGLSLLCIAAVQALYWFVVQPS
                     LFAKAPPVETLEVLDPHWAALPAPTRSAIAAARFEPVELPYDGCCAPGYGVLRTEFEL
                     TAIPEAGLELATPRGADNAHILLNGAWIHQPGRIALPDITYHGTLRSVIRIPASALKI
                     GRNRLEAVMVRSATPYMDAWPPQLAPAPTLSQGTARSLFLGNEYRVLSSSIAAIVALL
                     ALMLLLRSEQRALPFWGFVLAAAWGLRTHYYLWSDPPFSGPGRLAYYFTVTGLVPVAW
                     ANLANEWTGRPIRAVRWLATLGGLAMIAVMVWALLAGWDGAYDLASQITDLGGVALGV
                     IACAVLIWHLVTRRDDRHWEVAILILVITLLTSDYLKQYTQEKSNVFMANTVPLLLIG
                     FAVAFLARNVRLFRSVSAINQLLAGQLKEREAELAVQYARQEELARRETLVGERQRLM
                     RDMHDGIGGQLMSLLFAARQKVLPPEELAQSLQAVIDELRLIIDSLDTVGESLSAALA
                     SFRGRIEPRLSAAGITIHWSNTLPDALPELPPRTILQVFRIVQEAITNAIKHSGTRSL
                     AVSVALADAPGRPLEIRIADQGQGFDPVTALGGRGLESMEARAAAIGGTLTVTPSAEG
                     TAVILTVPIPAEGAA"
     gene            8235..11579
                     /locus_tag="VFV30_06600"
     CDS             8235..11579
                     /locus_tag="VFV30_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX4847795.1"
                     /translation="MDLSSAIALTLGLVAGLFALATLVERRAAQLDARPRLRHLAYTL
                     ALGVYCSSWTFYGAVGSAVRDGWNYLPIYLAPILLLLLAPALLRRLAEAVAQERATTV
                     SDFIAARFGHDVVVARLVTVIALLGSIPYIALQLRSIGNALSLVSGQAVVVPAMIGAA
                     ALLALFTMLFGARRYEVAGRSEGLVYAIGIDSLVKLIALAAVAALAVAVIRGLDPASL
                     SAATAQLAGQFQPERITLDTAVIMLIAGGAILTLPRQFYMGLVEAREPGDFARARHGQ
                     AAYLAIMAALVVPISLAGIAVLGPAASPDSYVLQLPADQGTALVIALALLGGVSAAAS
                     MAIVDSTALATMVSNDLVFPALLRGQRQAPPGAIGARMLAVRRISVVAIMALALAWGL
                     QVSASNSLASIGLIAFAAMAQFAPHLLLAAYGSGRDPLAARASLTAGLVLWLYTLALP
                     PILPAGGLEALSGTPADPLRLFGIGSASPLVHGVLWSLGVNLAVYGLLSARTMRAPKL
                     PRFLRGQSAVTDLAGLVDLTAGFVGRERAELEFPQVRRGQPVDRRSARRAQDLIAGVV
                     GAASARALVTSALAGGTMSLPEVTRLLDEGGQSLRFSRQLLAATFENIDAGISVVDAD
                     MNLVAWNSRYLELFDYPLGMVRVGVPVADLIRHNAQRGDFGPGDAEFHVQKRLGHLRR
                     GLEHSFERLRKDGRVIKTVGGPMPGGGYVMSFTDISEEARVREELRRTLDELEHRVTD
                     RTSELSEANRRLAESDRDKTRFLAAASHDLLQPLHAARLFTAALEREVDERPRGLVHR
                     IESAIVAAEDLLRALLDISKLDAGGVTPRPEPVDLAGFLTDLTDSFRPRAEEKGLTLR
                     LAAVPGRVETDPALLRSVMQNFLTNAVRYTRSGGIVVGVRRRGAMWRIDVIDSGVGIA
                     PDKLDAVFGEFTRLGDVEVEGLGLGLALVQRIARLLGGRISLSSRPGRGSRFSFALPA
                     ADGLAAPAEPRAAERAAEARPLTVLVVDNDARIVEATAALLAGMGHTPLGAGDIAGAL
                     GLCDRADAVLADYQLDNGEDGLSLIAQIRARRPGLPVRLITAETGAAMRARAAAMKVG
                     VLAKPVDPAKIGRFLAEASVAEIEAE"
     gene            complement(11553..12185)
                     /locus_tag="VFV30_06605"
     CDS             complement(11553..12185)
                     /locus_tag="VFV30_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4847796.1"
                     /translation="MTGTVLIADDHPLFRQALSLAVQRVSPAARLVEAGTLAAAARAV
                     EAASDLRLILLDLKMPGAVGYSGIALLHAERPEVPILVVSSAEGEAAAQEARAFGAIG
                     FLRKDSDLSAIEAAIAKALSGTAPAPASGAAPAPEEQVRQTVAGLTPTQLKVLLAVLD
                     GQLNKQIAFSLGMSEATVKAHMTAIMRKLDVRNRTQAALVARSLGLDLSH"
     gene            complement(12182..>14018)
                     /gene="acs"
                     /locus_tag="VFV30_06610"
     CDS             complement(12182..>14018)
                     /gene="acs"
                     /locus_tag="VFV30_06610"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX4847797.1"
                     /translation="RAEAQRLDWIKPFTTVKETSFHEADFGIKWFADGSLNLAANALD
                     RHLAQNGESVAIIWDPDSPDEPARHITYRELHADTCRFANLLKARGVKKGDRVTIYLP
                     MVPEAAVAMLACARIGAIHSVVFGGFSPDSLASRIEDCDSTVVLTADEGLRGGKAVPL
                     KANVDEALTKVTSVDTVIVLKRTGANVPMVPGRDVDWHEGVAAQSADCPAEEMNAEDP
                     LFILYTSGSTGKPKGVLHTTGGYGVWASMTHQYVFDYRPGQIYWCTADVGWVTGHSYI
                     VYGPLINGATTLMFEGVPNYPDPSRFWQVIDKHKVEIFYTAPTALRALMREGDDFVKR
                     TSRSSLRLLGSVGEPINPEAWEWYHRVVGDGRCPIVDTWWQTETGGVLISPLPGATAL
                     KPGSATKPFFGVQPMLVDNEGVPIKGPGDGCLVITDSWPGQMRTVWGDHERFFQTYFS
                     TFKGLYFTGDGCRRDADGYYWITGRIDDVINVSGHRMGTAEIESALVAHAKVAEAAVV
                     GMPHDIKGQGIYAFVTTNADVEPDEALRRELVQWVRHEIGPIATPDVIQFAPGLPKTR
                     SGKIMRRILRKIGENDVGNLGDTSTLADPSVVDHLLANRPSLTAA"
BASE COUNT         2069 a         4885 c         4841 g         2223 t
ORIGIN      
        1 ggcgtgaacc tgggcgtgct ggcccagcac ggctcgctgt tcgtcacccg gatgaccctg
       61 ttcgactatt accgcgagcc ggccgaggcg ctggccggcg cggccaagct gtgggacatg
      121 gtcggcagcg gcaagctgac cgtcgccatc ggccagacct atgccctgac cgacgccgcc
      181 caggcccacc gcgatctgga agcgcgccag accaccggat cgaccctgct gctaccctga
      241 gccaagggaa aaccggcccg ggcgggggcc cgggccggcc ccggggctag acgccgcgac
      301 ccaagtgggt cttgcgccgg ttccagccga tccaggccgc gccgagtgcc gcgagggcta
      361 gcacgatggc accgcccagc cgcagccagg ggttgaaggc ccggacactg tcggtcagct
      421 tggcgacctt gggatcggcg ggcagatagg taacgctgat ggccgagccg atcgcgtggg
      481 cctcgaaccg ttcgaacgag ccggcgttga tccgcacggt ggtgtcggtc ggcccgctcg
      541 ccgccgcgcc gctgccgcca atggtgagcc cggccacgat ctctgccccg ctgcgcggct
      601 tggccgttac cgcggcgtcc gcggccggcg cggtaccaaa gggaaccccg gcgcgcgaat
      661 tgaaggcaat cacgaaatag cggttttccg aagtgttgtt gcgcttggcc cggctgctgg
      721 tggtgctttc cacctccttg ctgcggatca ccccgtccac cgtgcgccct tcgctgcgca
      781 aggcccggta ttcggggatc tggccggcca gcagcacgcc cagccacagc acggccccca
      841 gccccatgac cattgccgcc accagccacc agccgccggg gcccatctgt tgccgtccgc
      901 tcatcgccat ctccgtgtcc ctcctacccg atgcacgagc agggaatgta ggtgccgtag
      961 gtctcggccc cgttgaactt cgcattcccg gccgagaacg cgccgccgac atagggctgg
     1021 ctgtaggaat agccctggat gtcgcagaat tccgccccgc gcgagacgat cgccgcgtcg
     1081 atctggcggt gcaggatgat cccggtgacc ggatggcgga cgatcgtcca gccgctgtgg
     1141 gtgatccgca cccccaccgg cggagcgctg ggccggttcc agaccgaacg ggcgtgggac
     1201 aggatcgtcc cttccagcgc cgggttggcc tgcaccgcct gggttccgca gattttcgga
     1261 tcggaccggt tgtaggcccc gggccgcacc gtcagggtga attcaccctt ggccacggcc
     1321 gcgccgcgcg ccttttcgac gaagttgttc ttgccgctgg tgccttcgtt gacgatcacc
     1381 accgggatca tctccagcgt gatcttgtaa gtgcccggcc ccagatcgcc cttgcgggtg
     1441 gcgcgggaca gcagctcgcc aaagccctcc tgcccgggga agaaccgctc gccccggctc
     1501 agcagctggc cgcgccaggt ggtccaggtc ttgtattcct ccgccgtgcc ccaggggcgc
     1561 tgcgacactt cgtagggccg cccgtccacc gtgaagagca tcttgtactg ggtgaaatag
     1621 gcggtgttgt gatcgtccat ccccgggacc cggccataga gccgcgtcgg ggcgggttcg
     1681 gccatgtaga cccggaaata gatctcgtcg cccagattga aggcggactt cagcatcccg
     1741 gcgttcatcc ggtcgagcgg catgtcctgg cccgaaaaga cgatccggcc gatgttctgc
     1801 cggtgcaggt cgctggtgat cccctggctg gcgccctgcg gcgcggccgg ggcggaaaag
     1861 ctggtgccct ggttggaatt gggctgttcc tgcttgccgc cgcggtcggg gcgcgccgga
     1921 cggggcgttt tcgccccgcc gatcgcatcg ccgaccgaag ggatctgggc aacggtggcg
     1981 ctggtcgtca ggccggcgat cagggcgaat tcggccagga tggcaagttt gcgcatgggt
     2041 gtcctcccgt cagaagccaa ggctgaagcc gacccggccg aggtgccggt cgctggtgcc
     2101 gaactgtccg gaatagccaa tgtggagctg ggccgagcgg cccaccgcca cgtcgagcgc
     2161 cgcgccgaga tagccgtgca ggctgtcccg cgtgtcgccc tgcagcacga ccccggcgtt
     2221 gctggcggcg aagctgaccg ggatcagccg gtccccgttg acgcccagca cccgtccgcc
     2281 gaactccacg cggaaatcgg ccccgccgcc cagcggacca aagtgccgcc cgatccgcag
     2341 gcccgcttgc ccttccagcg tgtcggccga gaagtcgccc agcgacagcc gcagcgggct
     2401 ggtgccgctt tcggtgtagc cgtcctgatc ctgccggatc caggtgccgc gcagggttcc
     2461 gcccagcgac cacttgccga tccgccccag cggcaggtcc agcccgatga agccgccata
     2521 gagggtcgat tccgtctcgc cctgcacggt cgcggaaaag ccgttgaagc tgacgttgcg
     2581 aaccgtgtcc tgatcgatcc ggcccagcag caccccgccg gtcagcttgg catcgccgat
     2641 gtcatgccgc agggtcaccc cgcccagcac gctcgattgc ttgcccccgc cgccgcccgc
     2701 gcccagctcg atatcgccgc gggcccagcc ggccgacagg cccagcgtgg tcgtctcgcc
     2761 cagctgaaag gttaccccgc cgctcaggcc atagctgtcg tgcccatagg ccagcgtctg
     2821 cccgctggcc gaacgctgcg catccccggc aaagccatcc agccacagcc gggtgccgcg
     2881 tggcatgcgg cggattgccg actcgctcag gtcggcgaag cccgatccgg tttccgccgc
     2941 cgcgaggaac tgggcgttga aggtggaggg ccgcgcggtc agcaccgagg gcgccatcag
     3001 cgcgctgctg ggctgataga tcaccgccag cggcagctgc gcgccgaccg tgtcgaccgt
     3061 cgcaaaggtg ccggtgatcc cgccggacgc ggtcaggaag gttccgccgt tgccctctgc
     3121 cccgcccacc gagatgaacc gcagggtccc gccgttgagc gagacgctgc cgttcacggc
     3181 cagcaggtcg atgttgccgt tggcatcgaa ctcgatctcc agcaccgacc cggcgttgtg
     3241 ggtgtagttg ccgttgatgg tcagcgtgcc gatcgaattg cccggcgcga ccgtgccgtt
     3301 gttggtcagg ctgccggtga tcgaacccgt cccgtcaagc cgcgcggcgc tgccgacgac
     3361 ggcgttggcg ctgatcggcc ggttgacccg gaccgaaccg gcatcgacgg ccagggtccc
     3421 gaccgtgaac agatcgacga aggtcagccg cccggccccc agcttgcgga aggtgctgcc
     3481 ggcaaaaccc gagacccgca ggtcgagatc gtggttggtg gccgtggtca gctcaaacgt
     3541 ggcctgaccg ctcaggttga tgacgctgcc cgacccgccg aaattgcccg aggaggtgcc
     3601 cgcgagcgtt ccggcgttga ccgaccagat cgcggtgaag ccctggttga ggcccgtcat
     3661 ggtggtgcgg ccggttccgt ccttggtaac cggacccgat ccgaccagcg tgttgccgaa
     3721 cgtgatgtcg cccagcgaca gcccgccggc ggtgatcgtc accgtgccgg agccgagcgc
     3781 gttctggtgg ccagcgtgga gccagccgcc ctcgatccgg gtttgtccga agaagccctg
     3841 attgtcgcgg ctgatcacca ccgcgccggt gccctgcttg accagggtgc catagccatt
     3901 gagcgtgttg tggaagatct ggctgggatc gagcggatcg gggaagctgc ttccgccgat
     3961 gtcgagcacc agccggccgc caaagccgat cgagacgatc gccgggctgg ccgtattgcc
     4021 cccgcccagc acgcgcaggt cctggatccg cagatcgccg ctggtgacat tcaccaagcc
     4081 gccgatgctg ttgaagctgt tggtgatcgt gctcgacgtg cccgagacaa cgatgatgcc
     4141 gttgcccgac aggcggttgc cgagagtcac gtcgttgagg tgcagccagc cgttgttgac
     4201 cacttcggcc agcccgaagg cctgcgcatg gctcacccgc aggacggcgg aggtgttgat
     4261 cacccggccg ctgaacgaag gcgaagcgga agcgaaggtg tagtcgccgg tgttgatgaa
     4321 ctcgatctcg ccatcgccca gcagcacatg ctgaaactgg tgggttccgc tatcgccgac
     4381 caccagccgg ccggcgccga gcaccccgcc gcgcaggtcc agcgtgctag tcgcctgccc
     4441 caacgcggtg aaggtgttgg cggtcagggt gacgcccgta tccacgcgaa tgtcctggaa
     4501 ggcatggctg ccgcccagct gccagttgga gccgccgtaa aggtgcagcc gctcaaaatc
     4561 gctggtggag aaggcgatct ggccatcgcc aaagccgcca tcgccctcca gcgtatcgaa
     4621 cccggccccg ccccggatcg aaatcgccga ggcgagcggc gtggtggtgc tcagctcggt
     4681 cagttcggtc cggctggaaa tgaccacggt gtcgtcgccg tcgccggcat cgagataggc
     4741 aatcgtgccg cccgaatccc ggccgatcat gcgcccgtgc aggaagaact gctcgttgct
     4801 gctgccgccc tggaccagcg gcccgttcag cgtcggcgcg gcgacgatcg cccctccgcc
     4861 ggacagctcg aaccgggcgt tggtgccgct cagcgaaacc gtgctggcgg tggtcagctg
     4921 gccgccatcg atcgtcagcc ggacgccgtt gccgctgagc gcaatggtgt tggcggcatt
     4981 ggtgttggtc agcgttcccc cgttcagtgc cagcattacc ggcccggtcg cgccggaatt
     5041 gcccaggttg atggacaggt cgttgaccgt cgccaggctg ttcaggatca tgaaggcccg
     5101 gttgccggtc agcgccacgg tatcgccggg attgccctgc accagcgcgt tgggatccac
     5161 caccacggtc acgtcgtcgg ccgaggaatt gatgctgagg ccgttggtgg cgttggtgca
     5221 agtgacggtg gaaccggcgt tggggctggc aacggtgccg ctgcccgatg tcagcgcgca
     5281 ggacgcccag gcctgcccag ggtccagcag gcaccacaag gccgcggtgc ccagcgccgc
     5341 gcgccggacg tgccgcgtgg atttcgggcg gagattcgaa gacatgccat gctccctgaa
     5401 caacgcgaag gatcgatcgg catgttcagg caggcgcgcg cacctgggta tgacataggt
     5461 atgtcagggg cggtttaacc caggttcaac ttgccgctgc gcagcgcctc gtaaaccgcc
     5521 tcgctgcggg tgtggacgtc aagcttgcgg tagatcgagc ggacgtggtt gccgaccgtc
     5581 agcggcgaaa tcgcgaggat ccgggccgcc tccttgtagc tgtagccctt ggcgaagagc
     5641 tgcagcagct cggtctcgcg cggggacagc ggggttgcgg catcctcgcc cggggtgggc
     5701 gcgggaaagc tccgcaaccg ttcgagcagg tgaaccgccg ccgccgcgct gatcggcgcg
     5761 ccgccggcca gcgcggcctc gatcgcggcg aggatatcgg gagtggcgct gtccttgagc
     5821 agatagccat cggcacccgc ctcgattgtg cggaccacgg tgtcgcggtc gccaaagctg
     5881 gtcaggacga tcactttcgc ctcgcccgcg gcgcggacct ggggaatgaa ggacgccccg
     5941 ttgccgtccg gcaggccaag gtccagcaga tagacatcga ccccccggtc cagcagcccg
     6001 cgggccgccg ccagcgaagc cgccgtgcca accagcgcat agccgccgtc ggccgcgatc
     6061 acgtcggcca gatagccgcg cacgagcgga tcgtcctcga cgatggcaac ctgggtctgc
     6121 atcacgccac cctgccacgg tgccgatgcg gcgcaaactg acgcggatat gtcatgccgc
     6181 gccctccgcc ggaatcggca cggtcaggat cacggcggtc ccctcggccg agggcgtcac
     6241 ggtcagcgtt ccgccgatcg cggcggcccg cgcctccatg ctctccagcc cgcgcccgcc
     6301 cagcgcggtg accggatcaa agccctggcc ctggtcggcg atccggatct ccagcgggcg
     6361 gcccggcgca tcggccagcg cgacggaaac cgccaggctg cgcgtgccgg aatgcttgat
     6421 cgcgttggtg atcgcctcct gcacgatccg gaacacctgc aggatggtgc gcggcggcag
     6481 ctcgggcaag gcatcgggca gagtgttgga ccagtggatc gtgatccccg ccgcgctcag
     6541 ccgcggttcg atccgccccc ggaacgaggc cagcgccgcg ctgagcgatt cccccaccgt
     6601 gtcgagcgag tcgatgatca gccgcagctc gtcgatcacc gcctgcaggc tctgcgccag
     6661 ctcctcgggc ggcaggacct tttgccgggc ggcgaacagc aggctcatca gctggccgcc
     6721 gatcccgtcg tgcatgtcgc gcatcagccg ctggcgctcg cccaccagcg tctcgcgccg
     6781 ggccagctcc tcctgccggg cgtactgcac cgccagctcg gcctcgcgct ctttcagctg
     6841 gccggccagc agctggttga tcgcgctgac cgagcggaac aggcggacgt tgcgggccag
     6901 aaaggcaacc gcaaagccga tcagcagcaa cggaaccgtg ttcgccatga acacgttgct
     6961 cttttcctgg gtgtattgct tcagatagtc gctggtcagc agggtgatca ccaggatcag
     7021 gatggccact tcccaatggc ggtcgtcgcg gcgggtcacg aggtgccaga tcagcaccgc
     7081 acaggcgatc acgcccagcg ccactccgcc gagatcggtg atctgcgagg ccagatcgta
     7141 ggccccgtcc catccggcca gcagcgccca gaccatcacc gcgatcatcg ccagaccgcc
     7201 cagcgtggcc agccagcgca ccgcgcggat cggacggccg gtccattcgt tggccaggtt
     7261 ggcccaggcg accggcacca gcccggtgac cgtgaagtaa taggccagcc gccccgggcc
     7321 gctgaacggc ggatcggacc acaggtagta atgggtccgc agcccccagg cggcggccag
     7381 gacgaacccc cagaacggca gcgcgcgctg ttcggaccgc agcagcagca tcagcgccag
     7441 caaggcgacg atggcggcaa tgctggagga cagcacccgg tattcgttgc caaggaacag
     7501 actgcgcgcc gttccttgcg acagcgtcgg cgcgggggcc agttgcggcg gccaggcgtc
     7561 catatagggg gtggcgctgc gcaccatgac ggcttccagc cggttgcgcc cgatcttcag
     7621 cgcactggcc gggatgcgga tcaccgaacg cagcgtgccg tgataggtaa tgtccggcag
     7681 cgcgatccgc ccgggctgat ggatccacgc gccgttgagc aggatatggg cgttgtccgc
     7741 cccgcgcggg gtcgccagct ccagcccggc ttcggggatc gcggtcagtt cgaactcggt
     7801 ccgcagcacg ccataccccg gcgcgcagca cccgtcatag ggcagctcga ccggctcgaa
     7861 acgggccgcg gcaatcgcgc tgcgggtcgg cgcgggcagc gcggcccagt gcggatcgag
     7921 cacctccagc gtttccaccg gaggcgcctt ggcgaacagg ctgggctgga ccacgaacca
     7981 gtagagcgcc tgcacggccg cgatgcacag cagcgacagg cccaggctgc gccggtccac
     8041 cggggcacgg tttgcccaga acgctgccac ccggtccgcc aagccgccca tccttcgtcc
     8101 ttccgcggga atagcaccat attcatggca tgcgggcaat gccatgttcg tgtcatggac
     8161 aggccgatca gcccgattgc ccccgctcca attgccgtta caacggacgc gggagagggg
     8221 gagtaagcca ggccgtggac ctgagttccg ccattgcgct gacgctgggg ctggttgccg
     8281 gcctgttcgc gcttgccacc ctggtggagc ggcgggcggc gcagctggat gcgcggccgc
     8341 ggctgcggca cctggcctat accctggcgc tgggggtcta ttgctccagc tggaccttct
     8401 acggcgcggt cggcagcgcg gtgcgcgacg ggtggaatta cctgccgatc tatctcgcgc
     8461 cgatcctgct gctgctgctt gccccggccc tgctgcgccg cctagccgaa gctgtggccc
     8521 aggaacgcgc gaccaccgtg tccgatttca tcgccgcccg gttcgggcac gatgtggtgg
     8581 tggcgcggct ggtcaccgtg atcgcgctgc tcggctcgat cccttatatc gcgctgcaat
     8641 tgcgctcgat cggcaacgcg ctgtcgctgg tttcggggca ggcggtcgtc gtcccggcaa
     8701 tgatcggcgc ggccgcgctg ctggcgctct tcaccatgct gttcggcgcg cggcgctatg
     8761 aagtggcggg ccgcagcgag ggcctggtct atgcgatcgg gatcgattca ctggtcaagc
     8821 tgatcgcgct ggcggccgtc gcggcgctgg ccgtggcggt gatccgcggg ctcgatcccg
     8881 ccagcctgtc cgccgccacc gcccagctcg ccgggcagtt ccagccggaa cggatcacgc
     8941 tggataccgc cgtgatcatg ctgatcgcgg ggggcgcgat cctgaccctg ccgcgccagt
     9001 tctacatggg cctggtcgaa gcgcgcgagc cgggcgactt tgcccgggcc cggcacggcc
     9061 aggcggccta ccttgccatc atggcggcgc tggtggtgcc gatctcgctg gccgggattg
     9121 ccgtgctcgg cccggccgcc tcgcccgaca gctacgttct gcaactgccg gccgaccaag
     9181 gcaccgcgct ggtcatcgcc cttgccctgc tgggcggggt cagcgccgcg gcctcgatgg
     9241 cgatcgtcga ttccaccgcg ctcgccacca tggtttccaa cgacctggtt ttccccgcgc
     9301 tgctgcgcgg ccagcgccag gctccgccgg gcgcgatcgg cgcgcggatg ctggcggtgc
     9361 ggcggatctc ggtggtggcg atcatggcgc tggcgctggc ctgggggctg caggtctcgg
     9421 ccagcaattc gctcgcttcg atcgggctga tcgcctttgc ggccatggcc cagttcgccc
     9481 cgcacctgct gctggcggcc tatggcagcg ggcgcgatcc gctggccgca cgcgccagcc
     9541 tgaccgccgg gctggtgctg tggctctaca ccctggccct gcctccgatc ctgcccgccg
     9601 gcgggctgga ggcgctgtcc ggaaccccgg ccgatccgct gcggctgttc gggatcggat
     9661 cggcctcgcc gctggttcac ggcgtgctgt ggagcctggg ggtgaacctg gcggtctatg
     9721 gcctgctctc cgcccggacc atgcgcgcgc ccaagctgcc gcggttcctg cgcgggcaaa
     9781 gcgcggtgac ggacctggcc gggctggtcg atctgaccgc cggctttgtc gggcgcgaac
     9841 gggccgagct ggaattcccc caggtccgcc gcggccagcc ggtcgaccgc cgctcggccc
     9901 gccgcgcgca ggatctgatt gccggcgtgg tcggcgcggc ctcggcccgg gcgctggtca
     9961 cttcggcgct ggccggcggc acgatgagcc tgcccgaagt gacccggctg ctcgacgagg
    10021 gcgggcaaag cctgcgcttc tcgcgccagc tgctggcggc cacgttcgaa aacatcgacg
    10081 ccgggatcag cgtggtcgat gccgacatga acctggtggc ctggaacagc cgctatctgg
    10141 aactgttcga ctatccccta ggcatggtcc gggtcggggt gccggtggcc gacctgatcc
    10201 gccacaacgc ccagcgcggc gatttcgggc ccggcgatgc cgaattccac gtccagaagc
    10261 ggctgggcca cttgcgccgc gggctggagc acagctttga acggctgcgc aaggacggac
    10321 gggtgatcaa gaccgtgggc gggccgatgc ccggcggcgg ctatgtgatg agcttcaccg
    10381 acatttccga ggaagcgcgc gtgcgcgaag agctgcgccg gacgctggac gagctggaac
    10441 accgcgtcac cgaccggacc agcgaactgt ccgaagccaa ccgccgcctc gccgaaagcg
    10501 accgcgacaa gacccgcttc ctggccgccg ccagccacga tctgctccag ccgctccacg
    10561 ccgcgcggct gttcaccgcc gcgctggagc gcgaagtgga cgaacgcccg cgcggcctgg
    10621 tccaccggat cgaaagcgcg atcgtcgccg ccgaagacct gctgcgcgcc ctgctcgaca
    10681 tctccaagct tgacgccggc ggggtcaccc cgcggcccga gccggtcgac ctcgccgggt
    10741 tcctgaccga cctgaccgac agcttccgcc cccgggccga ggaaaagggc ctgaccctgc
    10801 ggctggccgc ggtgccgggg cgggtcgaga ccgatcccgc gctgctgcgc tcggtcatgc
    10861 agaacttcct gaccaacgcg gtgcgctata cccgcagcgg cgggatcgtg gtcggggtgc
    10921 ggcggcgcgg cgcgatgtgg cggatcgacg tgatcgacag cggcgtcggc attgccccgg
    10981 acaagctgga cgcggtgttt ggcgagttca cccggctcgg cgatgtcgag gtggagggcc
    11041 tgggcctcgg cctcgcgctg gtccagcgga tcgcccggct gctgggcggg cggatcagcc
    11101 tgtcctcgcg ccccggacgc ggcagccgct tttccttcgc cctccccgcc gccgatggcc
    11161 tggcggcccc ggccgaaccg cgcgcggccg agcgcgccgc cgaggcccgg ccgctgaccg
    11221 tgctggtggt cgacaacgac gcccgcatcg tcgaggccac tgccgccctg ctggcgggca
    11281 tgggccacac cccgctcggc gcgggcgata tcgccggtgc cctggggctg tgcgaccggg
    11341 ccgatgcggt gctggccgat taccagctgg acaacggcga ggacggcctg agcctgatcg
    11401 cccagatccg cgcccgccgg ccgggcctgc cggtgcggct gatcaccgcc gaaaccggcg
    11461 cggcgatgcg ggcgcgggcg gcggccatga aggtcggagt gctggccaag ccggtcgatc
    11521 cggccaagat cggccggttc ctggctgaag cctcagtggc tgagatcgag gccgagtgac
    11581 cgcgccacca gcgcggcctg ggtgcggttg cgcacgtcaa gcttgcgcat gatcgcggtc
    11641 atgtgggcct tcacggtcgc ctcgctcatt cccagcgaga aggcgatctg cttgttgagc
    11701 tgaccgtcga gcacggccag cagcaccttg agctgggtcg gggtcaggcc ggcgacggtc
    11761 tggcggacct gctcttccgg cgccggcgca gcgcccgatg cgggcgccgg ggcggtgccg
    11821 gacagggcct tggctattgc cgcctcgatc gcggagagat cgctgtcctt gcgcaggaaa
    11881 ccgatcgcgc caaaggcccg cgcctcttgc gcggcggctt cgccttcggc gctggagacg
    11941 accaggatcg gcacttcggg ccgctcggca tgcagcagcg cgatcccgga atagccgacc
    12001 gcgccgggca ttttcagatc gagcaggatc agccgcaagt cgctcgccgc ctccaccgca
    12061 cgggcggcgg cggccagggt cccggcctcg accagccggg ccgccgggct gacccgctgc
    12121 accgccagcg acagcgcctg gcggaacagc ggatggtcgt ccgcaatgag gacggtgccg
    12181 gtcacgccgc cgtaaggctg ggccggttgg ccagcaggtg atcgaccacg gacggatcgg
    12241 ccagggtcga ggtgtcgccc aggttgccga cgtcgttctc accgatcttg cgcaggatcc
    12301 ggcgcatgat cttgcccgaa cgggtcttgg gcaggcccgg cgcgaactgg atcacatcgg
    12361 gcgtggcgat cgggccgatc tcgtgccgga cccactgcac cagttccctg cgcagcgcct
    12421 cgtccggctc gacatcggcg ttggtggtga cgaaggcata gatgccctgc cccttgatgt
    12481 cgtgcggcat gcccaccacg gcggcctcgg ccaccttggc gtgggcaacc agcgcgcttt
    12541 cgatttcggc cgtccccatc cggtggcccg agacgttgat cacgtcatcg atccggccgg
    12601 tgatccagta gtacccgtcg gcatcgcggc ggcagccgtc gccggtgaag tacaggccct
    12661 tgaaggtgct gaaataggtc tggaagaacc gctcgtgatc gccccagacg gtgcgcatct
    12721 ggcccggcca gctgtcagtg atgaccaggc agccatcgcc cggtcccttg atcggcacgc
    12781 cctcgttgtc gaccagcatc ggctgaacgc cgaagaacgg cttggtggcc gaaccgggct
    12841 tgagcgcggt ggcgcccggc agcggcgaaa tcagcacgcc gccggtctcg gtctgccacc
    12901 aggtgtcgac aatcgggcag cgcccgtcgc cgaccacgcg gtgataccat tcccacgcct
    12961 cggggttgat cggttcgccg acgctgccca gcagccgcag gctggaacgg ctggtccgct
    13021 tcacgaaatc atccccttcg cgcatcagcg cgcgcagcgc ggtgggggcg gtgtagaaga
    13081 tctccacctt gtgcttgtcg atcacctgcc agaaccggct gggatcggga tagttgggca
    13141 ccccttcgaa catcagcgtg gtcgcgccgt tgatcagcgg gccgtagacg atgtagctgt
    13201 ggcctgtgac ccagcccaca tcggccgtgc accagtagat ctgccccggc cggtaatcga
    13261 acacgtactg gtgggtcatc gaggcccaga ccccgtagcc gccggtggtg tggagcacgc
    13321 ccttgggctt gccggtggag ccggaggtgt agaggatgaa cagcgggtct tccgcgttca
    13381 tttcctccgc cgggcaatcg gcgctctgcg cggccacgcc ctcgtgccaa tcgacatcgc
    13441 ggcccggcac catcggcacg ttcgccccgg tgcgcttcag tacgatcacg gtgtcgaccg
    13501 aagtgacctt ggtcagcgcc tcgtcgacat tggctttcag cggcacggcc ttgccgccgc
    13561 gcaggccttc gtcggcggtc agcaccacgg tgctgtcgca gtcctcgatc cggctggcga
    13621 gcgaatccgg gctgaagccc ccgaacacga ccgagtggat cgccccgatc cgcgcgcagg
    13681 ccagcatggc aacggcggct tcgggcacca tcggcaggta gatggtgacg cggtcgccct
    13741 tcttgacgcc gcgcgccttc agcaggttgg cgaagcggca ggtatcggcg tgcagttcgc
    13801 ggtaggtgat gtggcgggcg ggctcgtccg ggctgtccgg gtcccaaatg atcgccacgc
    13861 tctcgccgtt ctgggccagg tgccggtcca gcgcgttggc ggccaggttc aggctgccgt
    13921 cggcgaacca cttgatcccg aaatcggcct cgtgaaagct ggtctccttg accgtggtga
    13981 acggcttgat ccagtccagc cgctgcgctt cggcgcgc
//
LOCUS       DASUDV010000303         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1291251,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000303 DASUDV010000000
VERSION     DASUDV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291251"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..399
                     /locus_tag="VFV30_06615"
     CDS             151..399
                     /locus_tag="VFV30_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847798.1"
                     /translation="MKGMGGGEGEDCPFQFNFDPATFKVGDTVSYRVSSMEDWPFVGT
                     LLEVHADHVVISPGPAEPDARYKGTRESRPMVDGSEIG"
     gene            complement(815..1768)
                     /gene="purN"
                     /locus_tag="VFV30_06620"
     CDS             complement(815..1768)
                     /gene="purN"
                     /locus_tag="VFV30_06620"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX4847799.1"
                     /translation="MAERAKVAVLVSGGGTNMAALLYASRAADCPYEIVLVGSNNPDA
                     GGLKLAAAEGVPTFALPHKGMARADHDAAMDAAIRASGAQFVALAGYMRILTPEFVSG
                     WDGRMLNIHPSLLPKYKGLHTHERALEAGDSHGGCTVHLVTAELDDGPILGQTPVAVL
                     PGDTAESLAARVLIAEHQLYSRCLADLVSRETSPEWLTQQVRTRAMALPEAEETLSHG
                     MPCFGIIKGKKFAWVSLDHHGDGKTALLVKISGPEEQAQLIEMDEARYYRPAYFGDGW
                     IGIRLDLGDTDWDAVADWLARSWRSVAPKKLTALMDAADGF"
     gene            complement(1761..>2339)
                     /locus_tag="VFV30_06625"
     CDS             complement(1761..>2339)
                     /locus_tag="VFV30_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX4847800.1"
                     /translation="GDKVASGDVLLGLASSGVHSNGYSLVRRLAADKGWKLDRPALFD
                     PDRLLIDHLIEPTRIYVKSLLPVIRSGRIHALAHITGGGLLENIPRVLPEGLHARVDA
                     GAWEQPRLMAFLQAQGNIEPAEMARTFNCGIGMVLAVSADDAASVQADLEAAGETVFV
                     IGAIEPGARGCTVQGSAEDWSARETWEASHLG"
BASE COUNT          365 a          754 c          805 g          415 t
ORIGIN      
        1 tgatcgacga gatcgccgcg atcatcgcca aggccaaggc cgacgcctga ccgaccgcga
       61 tgccgaccgg ataggggctg ccgggcagac cggcagcccc ttttcgtttg gcggccgctg
      121 tcctatcctg ccggcaaagg agaatgcggc atgaaaggca tgggcggcgg cgagggcgag
      181 gattgcccgt tccagttcaa tttcgatccg gccacgttca aggtcggcga tacggtcagc
      241 taccgggtca gctcgatgga agactggcct ttcgtcggca ccctgctcga agtgcacgcc
      301 gatcacgtgg tgatcagccc cggcccggcc gaacccgacg cgcgctacaa gggcacgcgg
      361 gaaagccggc cgatggtgga cgggagcgag atcggttagt ggcgcattgc gcaaagtggt
      421 gctgtcgtcc aacttgatgc gatggacttg catccaaacc gacgagacga gccagactgg
      481 ctaaagatca agcggcgtaa cgagactgct gagcgatcat ggagttacgg gggctggacg
      541 gcaatgacgc tgtttcctgt tccctacgca gcgatacaag ccgttctgcc aagcgtgacg
      601 ggggctcttc ttgccgctgc cacggctctc actgctttgg caattgcggt ttatctgcgt
      661 tctgagaagg gcacattcac ccgtggctgt gccgtttgga gcgcgccggt tcatgggctg
      721 gctgcggctg ttttcctaac catgatgctt cagtagatcg gggtgaattc gccgtctctg
      781 ttgatcggca atggtccagt tggatgcgcg ccccctaaaa cccgtctgcc gcatccatca
      841 gtgcggtcag cttcttcggt gcgacgctgc gccaggaccg ggccagccag tcggccaccg
      901 cgtcccagtc ggtatcgccc agatccagcc ggatgccgat ccagccgtcg ccgaaatagg
      961 ccgggcggta atagcgcgct tcgtccattt cgatcagctg ggcctgctct tccgggccgc
     1021 tgatcttgac cagcagcgcg gtcttgccat cgccgtggtg gtccaggctg acccaggcga
     1081 acttcttgcc cttgatgatc ccgaagcacg gcatgccgtg gctcagcgtt tcctcggctt
     1141 ccggcagggc catggcgcgg gtgcggacct gctgggtcag ccattcgggc gaagtctcgc
     1201 ggctgacgag gtcggccagg cagcgcgaat agagctggtg ctcggcgatc agcacccgcg
     1261 cggccaggct ctcggccgtg tcgccgggca gcacggccac cggggtctgg cccaggatcg
     1321 gtccgtcatc cagctcggcc gtgaccaggt ggacggtgca gccgccgtgg ctgtcgcccg
     1381 cttccagcgc gcgttcgtgg gtgtggaggc ccttgtactt gggcagcagg ctggggtgga
     1441 tgttgagcat ccggccgtcc cagccgctga cgaattccgg ggtaaggatc cgcatgtagc
     1501 cggccagcgc gacgaattgc gcgccgctcg cccggatcgc cgcgtccatt gccgcgtcat
     1561 ggtccgcgcg ggccatgccc ttgtgcggca gggcgaaggt cggcacccct tcggccgcgg
     1621 ccagtttcag gccgccggcg tcggggttgt tggagccgac cagcacgatc tcataggggc
     1681 agtccgccgc gcgggaggca tagagcaacg ccgccatgtt ggtgccgccg ccggaaacca
     1741 gcacggcgac cttcgcgcgc tcagccaagg tggctggctt cccaggtttc gcgggcggac
     1801 cagtcctcgg cgctgccctg cacggtgcag ccgcgcgcgc cgggttcgat cgcgccgatc
     1861 acgaagaccg tttcgcccgc tgcttccaga tcggcctgga ccgaagcggc atcgtcggcg
     1921 ctgaccgcca gcaccatgcc gatcccgcag ttgaaggtgc gggccatttc ggccggttcg
     1981 atgttcccct gggcctggag gaaggccatc agccgcggct gttcccaggc cccggcatcg
     2041 acccgggcgt ggagcccttc gggcaggacg cgggggatgt tttcgaggag gccgccgccg
     2101 gtgatgtggg ccagggcgtg gatccggccg ctgcggatca ccggcagcag gcttttgacg
     2161 taaatccgag tcggctcgat caaatggtcg atcagcaggc ggtcgggatc gaacagggcg
     2221 gggcgatcca gcttccagcc cttgtccgcg gccagccgcc ggaccagcga atagccgttg
     2281 gagtggacgc ccgaggaggc gaggccgagc agcacgtcgc cgctcgccac cttgtcccc
//
LOCUS       DASUDV010000304         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_540049,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000304 DASUDV010000000
VERSION     DASUDV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540049"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1267)
                     /gene="lysA"
                     /locus_tag="VFV30_06630"
     CDS             complement(5..1267)
                     /gene="lysA"
                     /locus_tag="VFV30_06630"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX4847801.1"
                     /translation="MDHFAYKNGVLHAEDLPLTAIAEAVGTPVYVYSRATLERHARVF
                     RDALSILPRVHIAFAIKANPNLAVLRVMRREGLGADVVSGGEMARALAAGIPAEDIVF
                     SGVGKQHHEMIAGLEAGIGQFNLESEEEGIELAELAAERGLTARCALRVNPDVDARTH
                     AKISTGKAENKFGVPIDRGVEIYSRLAALPGLEMRGIAVHIGSQLNSLEPFEEAFAKV
                     GALIADLRAAGQSVTHADLGGGLGVPYKAGEVFPTPEAYAAMVARVTADWDVTLMFEP
                     GRVICGNAGVLLTKVVRVKRSSNQVPFVVVDAAMNDLARPAMYGAWHDFEAVKPTGER
                     MTAHIVGPICETGDTFAMDRETDAIGVGDLAVFRTAGAYGATMASSYNSRGFVAEVMV
                     DGDKWAVVADRILPEAITAAERVPDFLD"
     gene            complement(1274..1519)
                     /locus_tag="VFV30_06635"
     CDS             complement(1274..1519)
                     /locus_tag="VFV30_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847802.1"
                     /translation="MRRLAVSLLLAVLAGCGATAPLKPKAGESLPVARYGREGRESAE
                     DLLKAAPQAAPERSVELRKRSEERLQDPFDLPPPDDE"
     gene            complement(1525..>2661)
                     /gene="argH"
                     /locus_tag="VFV30_06640"
     CDS             complement(1525..>2661)
                     /gene="argH"
                     /locus_tag="VFV30_06640"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX4847803.1"
                     /translation="DIHMTTESRLAELIGPAAGRLHTARSRNDQVATDFKLWVREAMD
                     AADAGLKALQVALVTRAGEHADSIMPGFTHLQTAQPVTLGHHLMAYYEMAARDRSRFA
                     DARARAGQSPLGAAALAGTGFPIDRQRTAAALGFDGPTRNSLDSVSDRDFALDYLMAA
                     AQTALHLSRLAEEFIIWASQPFGFVTLPDSLSTGSSIMPQKKNPDAAELVRGHAGRIV
                     GSLTSLMITMKGLPLAYSKDMQDDKPPVFEAAGLLTLCLAAMTGMVEGATFRTERMRA
                     AAELGYATATDLADWLVRQAGIPFREAHHITGAAVKLAESKGLALDQLALTVLQAIDA
                     RIDERVYAALSVEASVAARASYGGTAPDQVRARVAEARVVLGLE"
BASE COUNT          397 a          930 c          909 g          425 t
ORIGIN      
        1 gcgctcagtc caggaagtcc ggcacccgtt cggccgcggt gatggcttcg ggcaggatcc
       61 ggtcggccac cacggcccac ttgtcgccat cgaccatcac ttcggccacg aagccgcggc
      121 tgttgtagga gctggccatg gtcgcgccgt aggccccggc ggtgcggaac acggccagat
      181 cgcccacgcc gatcgcgtcg gtttcgcggt ccatcgcaaa ggtatcgccg gtttcgcaga
      241 tcgggccgac gatgtgcgcg gtcatccgct cgccggtggg cttcaccgct tcgaaatcgt
      301 gccaggcccc gtacatcgcc gggcgggcga gatcgttcat cgccgcatcg accaccacga
      361 agggcacctg gttggagctg cgcttgaccc gcaccacctt ggtcagcagc accccggcgt
      421 tgccgcagat cacccggccc ggttcgaaca tcagcgtcac gtcccagtcc gccgtgaccc
      481 gcgcgaccat cgccgcatag gcttccgggg tcgggaagac ctcacccgcc ttgtagggca
      541 cgcccagccc gccgccgaga tcggcatggg tcacgctctg ccccgcagcg cgcagatccg
      601 cgatcagcgc gccgaccttg gcaaaggctt cctcgaacgg ctccagactg ttcagctggc
      661 tgccgatgtg gacagcgatc ccgcgcatct ccagccccgg cagggcggcg aggcggctgt
      721 agatttccac gccccggtca atcggcacgc cgaacttgtt ctcggccttg ccggtcgaga
      781 tcttggcgtg ggtgcgggca tcgacatcgg gattgacccg cagcgcgcag cgcgcggtca
      841 gcccgcgttc ggcggccagt tcggccagct cgatgccttc ttcctcgctc tcgaggttga
      901 actggccgat cccggcttcc agcccggcga tcatctcgtg gtgctgcttg ccgacgccgg
      961 aaaagacgat gtcctcggcc gggatcccgg cggcgagcgc gcgggccatt tccccgccgg
     1021 agacgacatc ggcacccagc ccttcgcggc gcatcacgcg cagcacggcc aggttcgggt
     1081 tggccttgat cgcaaaggcg atgtgaaccc gcggcaggat cgagagggca tcgcggaaca
     1141 cccgggcgtg gcgttccagc gtggcgcgcg aatagacgta aaccggggtg ccaaccgcct
     1201 cggcgatggc agtcaagggc aggtcttcgg cgtggagaac gccgttcttg taagcgaaat
     1261 ggtccatggg aaattactcg tcgtcgggag ggggcaggtc gaacggatct tgcaaacgct
     1321 cttcggaacg ttttcgcagt tcgacgctgc gctccggcgc agcctggggt gcggctttca
     1381 gcaggtcttc ggccgattcc cggccttccc gtccgtaccg ggcgaccggc aggctctcac
     1441 ccgccttggg cttgagcggc gcggtggcac cgcaaccggc cagcacggcg agcagcaggg
     1501 aaacggcaag gcggcgcatc gggatcactc caggccgagc acgacgcgcg cttcggccac
     1561 ccgcgccctt acctgatccg gcgcggttcc gccatagctg gcgcgcgcgg cgaccgaagc
     1621 ctcgaccgag agcgccgcat agacccgttc gtcgatccgc gcgtcgatcg cctgcaggac
     1681 ggtcagggcc agctggtcca gcgccaggcc cttgctctcg gccagcttca ccgccgcgcc
     1741 ggtgatgtgg tgggcttcgc ggaacgggat cccggcctgc cgcaccagcc agtcggcgag
     1801 gtccgtggcg gtggcatagc ccagttcggc cgcggcacgc atccgctccg tccggaaggt
     1861 cgcgccctcg accatgccgg tcatcgcggc aaggcacagg gtcagcagcc cggcggcttc
     1921 gaacaccggc ggcttgtcgt cctgcatgtc cttcgaatag gccagcggca ggcccttcat
     1981 ggtgatcatc aggctggtca ggctgccgac gatccgcccg gcatggccgc gcaccagctc
     2041 ggccgcatcg gggttcttct tctgcggcat gatcgagctg ccggtcgaca ggctgtccgg
     2101 cagggtgacg aacccgaacg gctggctggc ccagatgatg aattcctcgg ccagccgcga
     2161 aaggtgcagc gcggtctgcg cggcggccat caggtaatcg agcgcaaagt cccggtcgga
     2221 caccgaatcg aggctgttgc gggtcggccc gtcaaagccc agcgccgcgg cggtgcgctg
     2281 gcggtcgatc ggaaagccgg ttccggccag cgccgcggcg cccagcggcg attggccggc
     2341 ccgggcgcgg gcatcggcaa agcgcgaccg gtcccgcgcg gccatttcgt aataggccat
     2401 caggtggtgc cccagcgtga ccggctgggc ggtctgcaga tgggtgaagc ccggcatgat
     2461 gctgtccgcg tgctcgcccg cgcgggtgac cagcgcgacc tgcagcgcct tcaacccggc
     2521 atcggcggcg tccatcgcct cgcgcaccca cagcttgaaa tcggtcgcca cctgatcgtt
     2581 gcggctgcgc gcggtgtgga gccgtccggc ggccggtccg atcagctcgg ccagccggct
     2641 ttcggtggtc atgtggatgt c
//
LOCUS       DASUDV010000305         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1688902,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000305 DASUDV010000000
VERSION     DASUDV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688902"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1028)
                     /locus_tag="VFV30_06645"
     CDS             complement(252..1028)
                     /locus_tag="VFV30_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4847804.1"
                     /translation="MSLPQTLAAARPIDNGFAVRIPENWMQGRTAYGGFSAALALVAA
                     QQMAPDLPPLRSATVNFVGPLAGEAEVRAKILRRGRNASWISTEVTGEGGVGLSATFV
                     FMGAVEASSLHLHDVPMPADVVPFDQAVPLPERAGPSFAPNLDRRFALPRSDAKVPEI
                     CWWERIKDAEGLDPMVALILLADALPPGVMPLTGPAPISSMTWLINLLTPLPQTHDGW
                     FLLRAVGNYAEKGCSSQDMAIWNTAGEAVAVGMQSIAIFG"
     gene            1076..1849
                     /locus_tag="VFV30_06650"
     CDS             1076..1849
                     /locus_tag="VFV30_06650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX4847805.1"
                     /translation="MATFIDLSIPITTKVVSDPPVMLPKIEYVDHCETWQQIALFFPG
                     LEKADLPDGEGWAVETLTLSTHNGTHMDAPWHYHSTTDSGASPAPTIDQAPLDRFFRP
                     AVKLDFSHLPHGTVVSAAEVEAELARIGHDLQPLDIVLIQSGAIYGTDNFTDQGVGLG
                     AEATLWLTERGVEVVGTDAWSWDAPFSHTARRWAETRDPSIIWEGHKAGRIRPYWQIE
                     KLMNLAALPPHGFTLSCFPVKIEGASAGWIRAVAIIEGP"
     gene            complement(1884..>2848)
                     /locus_tag="VFV30_06655"
     CDS             complement(1884..>2848)
                     /locus_tag="VFV30_06655"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847806.1"
                     /translation="PSSLAAEDFIADAYAVSVQNDVTYAQGKVGASGVSPHLRDLKLD
                     IYRPTSAGKPLSGRPAVVLAYGGAFHRGSKGTTRFEEDGASDSAMGDWCQTFAAAGYV
                     CLSIEYRLVPDDPALPDAIDPAGLVPKAMLKDPVATARIDVIRRRMGLPLLDDQSREQ
                     LWNTLFAAAQDMGNAVGYARSHARDLGIDPDRIAVGGFSAGGATAVNLAYGMGVPVQA
                     VLSLSGGVAGYDLRKTARAGMPPGLFVMGQNDLEGVQLGTRALLGALGAAGIRSETAW
                     MPGFGHFYPMGAPSLGGDMTRMTLKTRALRFLDRTLGGSKTAKP"
BASE COUNT          539 a          953 c          885 g          471 t
ORIGIN      
        1 tgtgctagcc gacgtggcca gtttcctcga tcaggaactg gcaggctgaa ccggggtggc
       61 acacgggtta caaaggttac accgcgcaac ccattcgatc atatttaaat cagcaggtta
      121 gatcgaaagt cacacttttc cataagcgga aagcctgact gttcaaagtg cagacactag
      181 gccgctcccg cgataatgac agcgcggcgc gatgcaggaa aaggctttgt tgcggccctg
      241 gtgcgcgcca cctagccgaa aatcgcgatc gattgcatcc ccaccgcaac cgcctcgccc
      301 gcggtgttcc agatcgccat gtcctggctc gaacagcctt tttccgcata gttgccgacg
      361 gcgcgcagca ggaaccagcc gtcatgagtc tggggcagcg gagtcaacag attgatcagc
      421 caggtcatcg aactgatcgg cgccgggccg gtcagcggca tcaccccggg cggcagcgca
      481 tcggccagca ggatcagcgc gaccatcgga tcgagccctt cggcatcctt gatccgttcc
      541 caccagcaga tttctggcac cttggcatcg ctgcgcggca gggcaaagcg ccggtcgaga
      601 ttgggggcaa agcttggccc ggcccgttcg ggcaggggca ccgcctgatc gaatggcacc
      661 acatcggccg gcatcggcac atcgtgcagg tgcaggctgc tcgcctcgac cgcgcccatg
      721 aaaacgaagg ttgcgctaag ccccaccccg ccctcgccgg tcacctcggt gcttatccag
      781 ctggcgttgc gcccgcgccg caggatcttg gcgcgcactt ccgcctcgcc cgccagcggg
      841 ccgacgaaat tgaccgtggc cgaacgcagc ggcggcagat cgggtgccat ctgctgtgcg
      901 gcaaccagcg ccagagcggc ggagaacccg ccataggcgg tgcgcccctg catccagttt
      961 tcgggaatcc ggacggcaaa gccgttatcg atcggccgcg cggcggcaag ggtttggggc
     1021 aggctcatcg cgctttccat tgacgtaaag ggaaagcagg tcaacactgc ttggcatggc
     1081 gacgttcatt gatctctcga tcccgatcac caccaaggtg gtgtccgatc cgccggtgat
     1141 gctgccgaag atcgaatatg tcgatcactg cgaaacctgg cagcagatcg cgctgttctt
     1201 tcctggcctt gaaaaggccg atctgcccga tggcgaaggc tgggcagtgg aaacgctgac
     1261 gctttccacc cacaacggca cccacatgga tgcgccgtgg cactaccatt caaccaccga
     1321 ttccggcgcc agccccgccc caacgatcga ccaggccccg cttgaccggt tcttccgccc
     1381 cgccgtgaag ctcgactttt cgcacctgcc gcacggcacc gtggtcagcg cggctgaggt
     1441 tgaggccgaa ctggcgcgga tcggccacga tctccagccg ctcgacatcg tgctgatcca
     1501 gtcaggcgcg atctatggca ccgacaattt caccgaccag ggcgtgggcc tgggggcaga
     1561 agcaaccctg tggctgaccg agcgcggggt ggaagtcgtc ggcaccgatg cctggtcgtg
     1621 ggacgcgccg ttcagccaca ctgcgcggcg ctgggcagaa acccgcgatc catcgatcat
     1681 ctgggaaggt cacaaggccg gccggatccg tccctactgg cagatcgaaa agctgatgaa
     1741 cctggccgcg ctgccgccac acggcttcac gctcagctgt tttccggtca agatcgaagg
     1801 cgccagcgcg ggttggatcc gcgcggtcgc gatcatcgag gggccatgag cgccggggcg
     1861 cattttcggg gcgactgacc cgatcagggt ttggcggttt tgctgccgcc cagtgtgcga
     1921 tcaaggaagc gtagcgcgcg ggtcttcagc gtcattctgg tcatgtcgcc gcccaggcta
     1981 ggcgcgccca tcggatagaa gtggccaaag cccggcatcc aggcagtttc gctcctgatc
     2041 ccggcggcgc ccagcgcgcc cagcagcgcg cgggtgccga gctggacccc ttcaaggtca
     2101 ttctggccca tcacgaacag ccccggcggc ataccggccc tggcggtctt gcgcaggtca
     2161 tagcctgcga ccccgcccga cagcgaaagc accgcctgga ccggaacgcc catgccatag
     2221 gccaggttga ccgccgtcgc cccgccggcg gaaaacccgc caaccgcaat gcgatcagga
     2281 tcgataccca ggtcgcgggc gtggctgcgg gcatagccca cggcattgcc catatcctgc
     2341 gccgcggcga acagggtgtt ccacaactgc tcgcgcgatt gatcgtcaag cagcggcagg
     2401 cccatccgcc gccggatcac atcgatccgc gcggttgcca ccggatcctt gagcatggcc
     2461 ttgggcacca gcccggccgg atcgatcgca tcgggcaggg ccggatcatc gggaaccagg
     2521 cggtattcga tcgaaaggca gacatatccg gcggcggcaa aggtctggca ccaatcgccc
     2581 atcgccgaat ctgaagcgcc gtcttcctcg aagcgggtcg tgcccttcga tccgcgatgg
     2641 aacgcaccgc cataggccaa caccactgcg gggcggccgc tcagcggctt tccagccgag
     2701 gtcggacgat agatatcaag cttcagatcg cgcagatgcg gcgatacacc cgatgcgcca
     2761 accttgccct gggcataggt gacgtcgttc tgcaccgaga cggcataggc atcggcaatg
     2821 aaatcctccg ccgccaggct cgatggca
//
LOCUS       DASUDV010000306         3939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_164602,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000306 DASUDV010000000
VERSION     DASUDV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164602"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VFV30_06660"
     CDS             complement(<1..1209)
                     /locus_tag="VFV30_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX4847807.1"
                     /translation="MAADPTKGYRPETLAVAHGYDPEMGMWAAKPPIYMTSTFVYPSA
                     QYAKDVHEAFFDGTGPLKGTTHHIYSRLGHPGLDFLEKRLAAMDGAEAAAAFASGMAA
                     HSAIALAFMRPGDTMIHGRPIYGGVDMLYNQFLTDFGIGGEAYLDGTDEAEVRRAADA
                     AMARGTLKLIVAETPANPTAAIVDLELMVRLADEIGARQGHRPLVVVDNTFLGPFAQR
                     PIELGVDLNMTSLTKYCAGHSDLLAGGVSGKAELIEKLRFQRVIWGSHLDPFTSWLVL
                     RSMESVAVRMERAMDNARAVAEWLRDHPKVAGVTYLGFLPEGSRPRAVYDRQCKAAGS
                     TFSFHLHGGEAEAFRMLDGLKLLKLAVSLGGSETLICHSASTTHYAVPREQRLAGGIT
                     DGTMRLSVGLE"
     gene            complement(1199..2416)
                     /locus_tag="VFV30_06665"
     CDS             complement(1199..2416)
                     /locus_tag="VFV30_06665"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4847808.1"
                     /translation="MSGIDFERYGALLATRKPGHTLPQAFYTDAASFDFDLAAVFGQT
                     WLMAGLECELPQPGSYMAMTVGKWPVIITRDQGGTVHAFHNSCRHRGSILCQPGQGSA
                     PKLVCPYHRWTYNLDGSLFAASRMPQGFDKSGHGLKPVALRLVAGAIFICLSDDPPPF
                     EEFAEQFAAYAGPLDFAHLKLAATDVMVEQANWKLVMDNARECYHCATGHPELSTSFP
                     VQFRGLFELSENQRAAEYFAQMEALGLPHAPQQGPWWQIGRFPLNEGSSTISMDGQHL
                     VGKLLVEANGGAVGSMRWAVDPHLFGHATADHVFTCSFLPVSATETHAFSKWYVHKDA
                     VEGVDYHLDELKELWVKTNQQDRELAENNQRGVTSPGYTPGPYSPEAESMALRFTDWY
                     CDKARSMIATHGS"
     gene            2505..3503
                     /locus_tag="VFV30_06670"
     CDS             2505..3503
                     /locus_tag="VFV30_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4847809.1"
                     /translation="MCHQSTVLSGWKEGKISKQTKIDRRWLPLNALRAFEAVGKQLSF
                     TGGAATLSVSQSAMSRHVSALEDLLGEQLFERDAGRLSLTPAGQELLAVVSKSFERIE
                     QTLNAIRDESVPARAMRLHVPPSFLQQLFMPMLGEFRREHPEIRIDICSANVTGLPPT
                     EIDMAIVYDRPNIDDWVTDLLWMVRVEPLCSPETAEAAKGKSMQEFLSGQELLHLKLD
                     NQPRDLLWSDYLRRNGVALTVSGGLAFDTSVALAKYAMASGGVMLGDVDMFAAEIKQG
                     LLVAPYDAVIEDGYGYYLKLRADDLGDPAVSAFRSWLIGQFANFGVHAAAPARAKR"
     gene            complement(3493..>3939)
                     /locus_tag="VFV30_06675"
     CDS             complement(3493..>3939)
                     /locus_tag="VFV30_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4847810.1"
                     /translation="RRGARVIAVTGADKAAQVRDLGADRLVDRGADLRAELGRDAVDV
                     VVDIVGGPQFPALLDCLRPGGRYGSSGAIAGPVVELDLRTFYLKDLRMFGCTVLDPEV
                     FGNLVGYIERGEIRPVIDQTYPLAEIVAAQQRFQEKRHVGKIVLTV"
BASE COUNT          647 a         1287 c         1342 g          663 t
ORIGIN      
        1 ttccagcccc accgacaggc gcatggtgcc atcggtgatc ccgccggcca gccgctgctc
       61 gcgcgggacg gcgtaatggg tggtgctggc cgaatggcag atcagcgttt ccgatccgcc
      121 caggctgacc gccagcttca acagcttgag cccgtcgagc atccggaacg cctcggcttc
      181 gccgccgtgc aggtggaacg agaaggtcga tccggcggcc ttgcactggc ggtcatagac
      241 tgcgcggggg cgcgagccct cgggcaggaa gcccagatag gtgacgccgg ccaccttggg
      301 atgatcgcgc agccattcgg ccacggcccg ggcgttgtcc attgcccgct ccatccgcac
      361 cgccaccgat tccatcgagc gcagcaccag ccacgaggtg aagggatcga ggtggctgcc
      421 ccagatcacc cgctggaacc gcagcttctc gatcagctcg gccttgccgc tgaccccgcc
      481 ggccagcaga tcgctgtgcc cggcgcagta cttggtgagc gaggtcatgt tgagatcgac
      541 gcccagctcg atcgggcgct gggcgaaggg gcccaggaag gtgttgtcga ccaccaccag
      601 cgggcggtgg ccctggcgcg cgccgatctc gtccgcgagc cgcaccatca gttccagatc
      661 gacgattgcc gcggtcgggt tggccggggt ttcggccacg atcagcttga gggtgccccg
      721 ggccatggcc gcatcggcgg cgcggcgcac ctcggcctcg tcggtcccgt ccagataggc
      781 ttccccgccg atcccgaagt cggtcaggaa ctggttgtag agcatgtcga cgccgccata
      841 gatcgggcgg ccgtggatca tcgtgtcgcc gggacgcatg aaggccagcg cgatcgcgct
      901 gtgggcggcc atgccgctgg caaaggcggc ggccgcttcg gccccgtcca tcgcggccag
      961 gcgcttttcc aggaaatcca gccccgggtg gccgaggcgc gaatagatat ggtgggtggt
     1021 gcccttgagc ggcccggtcc cgtcgaagaa cgcctcgtgc acgtccttgg cgtattgggc
     1081 cgaggggtag acgaaggtcg acgtcatgta gatcggcggc ttggccgccc acatgcccat
     1141 ttcgggatca tagccgtgcg caacggcgag ggtttcgggg cgatagccct tggtcggatc
     1201 agctgccatg ggtcgcgatc atgctgcggg ccttgtcaca ataccagtcg gtaaagcgca
     1261 gggccatcga ctcggcttcg ggcgaatagg gaccgggggt gtagccgggg ctggtcacgc
     1321 cgcgctggtt gttctcggcc agctcgcggt cctgctggtt ggtcttgacc cacagctcct
     1381 tcagctcgtc gaggtggtaa tcaacccctt cgaccgcgtc cttgtggaca taccatttgc
     1441 tgaaggcgtg ggtctcggtc gcggagaccg gcaggaagct gcaggtgaag acgtggtcgg
     1501 ccgtggcatg gccgaacagg tgcggatcga ccgcccagcg catcgagccg acggcgccgc
     1561 cgttggcctc aaccagcaat ttgccgacca ggtgctgccc gtccatgctg atcgtgctgg
     1621 acccctcgtt gagcgggaaa cggccgatct gccaccacgg gccctgctgc ggtgcatggg
     1681 gcaggcccag cgcctccatc tgcgcaaagt attcggccgc gcgctggttc tcgctcaatt
     1741 cgaacaggcc gcggaactgg accgggaacg aggtggacag ttccgggtgc ccggtggcgc
     1801 agtgatagca ctcgcgcgcg ttgtccatca ccagcttcca gttggcctgc tccaccatca
     1861 cgtcggttgc ggccagcttc aggtgcgcga aatcgagcgg gccggcatag gcggcgaatt
     1921 gctcggcaaa ttcctcgaac ggcggcggat cgtcggacag gcagatgaag atcgcgcccg
     1981 ccaccagccg cagcgccacc ggcttcaggc cgtggccgct cttgtcgaag ccctgcggca
     2041 tccggctggc ggcaaacagc gaaccgtcga ggttgtaggt ccagcggtga taggggcaga
     2101 ccagcttggg cgcgctgccc tggccgggct ggcacaggat cgagccgcgg tgccggcagc
     2161 tgttgtgaaa ggcgtggacc gttccgccct ggtcgcgggt gatgatcacc ggccatttgc
     2221 cgaccgtcat cgccatgtag ctgcccggtt gcggcagctc gcactccagc ccggccatca
     2281 gccaggtttg gccaaacact gccgccagat cgaaatcgaa cgaggcggca tcggtgtaga
     2341 aagcctgggg cagggtgtgg ccgggcttgc gggtcgccag aagcgcgccg tagcgctcga
     2401 aatcaatgcc gctcatggtc cccgtctcgc ctgttcggaa gcggtttgac ccagtgcctg
     2461 ccttgcgcca agtatggttt ttgcatggcc gtatctcaaa agttgtgtgc catcagagca
     2521 ccgttctcag cggatggaag gaggggaaaa tcagcaagca gacgaagatc gatcgccgct
     2581 ggctgccgct caacgcgctg cgggctttcg aggccgtggg caagcaattg agctttaccg
     2641 gcggcgcggc cacgctctct gtttcgcaga gcgcgatgag ccgtcacgtc agcgcgctgg
     2701 aagacctgct gggcgagcag ctgttcgaac gcgatgccgg ccggctttcg ctgacccccg
     2761 ccgggcagga gctgctggcg gtcgtcagca agtcgttcga gcggatcgag cagaccctca
     2821 acgcgatccg cgacgagagc gtgccggccc gggcgatgcg gctgcacgtg ccgccctcgt
     2881 tcctgcagca gctgttcatg ccgatgctgg gcgaattccg gcgcgagcat ccggaaatcc
     2941 ggatcgacat ctgcagcgcc aacgtcactg gcctgccgcc gaccgagatc gacatggcga
     3001 tcgtctatga ccgccccaac atcgacgact gggtgaccga ccttttgtgg atggtccggg
     3061 tcgaaccgct ctgctcgccg gaaacggccg aagccgccaa gggcaagtcg atgcaggaat
     3121 tcctgtccgg gcaggagctg ctccacctca agctcgacaa ccagccgcgc gatctgttgt
     3181 ggagcgatta cctgcgccgc aacggcgtgg ccctgaccgt cagtggcggg ctggccttcg
     3241 acacctcggt ggcgctcgcc aaatacgcca tggcctcggg cggggtgatg ctgggcgatg
     3301 tcgacatgtt cgcggccgag atcaagcagg gcctgctggt cgcgccctat gacgcggtga
     3361 tcgaggacgg ctacggatac tacctgaagc tgcgggccga cgatctgggc gatccggccg
     3421 tttccgcttt ccgcagctgg ctgatcggcc agttcgccaa cttcggcgtc catgccgccg
     3481 cgccggcgcg ggctaaacgg taagcacgat cttgccgacg tggcgcttct cctgaaagcg
     3541 ctgctgcgcg gcgacgatct cggccagcgg ataggtttgg tcgatcaccg gacggatctc
     3601 gccgcgctcg atatagccga ccaggttgcc gaacacttcg ggatcgagca cggtgcaccc
     3661 gaacatccgc aggtccttga ggtagaaggt gcggagatcg agctccacca ccgggccggc
     3721 gatggcgcca gatgagccgt agcgcccgcc agggcgcagg caatcgagca gcgccggaaa
     3781 ctgcggtccg ccgacgatgt ccaccaccac gtcaaccgca tcacggccca gttcggcgcg
     3841 cagatcggcg ccgcggtcga ccaggcggtc cgcgcccaga tcgcgcactt gcgcggcctt
     3901 gtcggcaccc gtgaccgcga tcacccgggc cccgcggcg
//
LOCUS       DASUDV010000307         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_164653,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000307 DASUDV010000000
VERSION     DASUDV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164653"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..2815)
                     /locus_tag="VFV30_06680"
     CDS             complement(125..2815)
                     /locus_tag="VFV30_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX4847811.1"
                     /translation="MRGTRPPLRHLAALIGAAFGVVGAGASAQAQAWSANEDDSLLLE
                     LRSGQYRLGEPLRGYQTPAGVCVDFADLIQALDLPVRLDKKSRRATGWLFAEDQRFTL
                     DRDSDTVQNVNGSRAIARDAIHDTPEGWCVDLKALSGWMGVRFRADLSNLAVVLESDR
                     KLPFLEAIERKSRAARLRAPKFAEFDLSKLPQAQTPYQNWRTPSIDVQIQGQWSRNMG
                     ARAQFEALASGEALGLSYTARLAGSTDNGADSLRLKLFRNDPSAELLGPLKATQVALG
                     DVESLRGALTAQTSYGRGAFVSNRPLNRPGRFGVTSLSGTLPAGWDAELYRNGELRAY
                     QADRGDGRYRFDDIELLWGENDFEVVLYGPQGQIKRERSSAPVGIESLPAGKTWYWAG
                     VVDAGRDLIDLSKTFADPNTGWRWGVGVERGIDRRTTAGVEYQSVMFAGRRRHYIEGT
                     VQRALGRMLLEVSGAQQLGAGRAFQAKALGRFGAIRYDAEALWVDGDFESELVEAQQR
                     REYGLRLSTSVKMGSWRLPVETGIRHFQTKTGAKVTEWLIRSALQLKRTSLTTELSRR
                     TARGPATALVGEDLGTKLNLIANTAIGPVRLRGAARFGLDGQQRGLENAQAVAETAIG
                     QSSTLRLGYEHDATSRRDEVMLGWVRQFNRFALRTEGRLDTRGRLTLGLTLGFSLGPD
                     PVDGGWRMSRQRLAESGQAAVEVFRDENGDGFRQAGEGVVEGVAAEAGFRHTDAPTNK
                     AGRAVIDGLTPYVPVLVSIDAGTLPDPLLQPKGQGVVVVPRPGVMAKVSLPLAPTGEL
                     EALLLGPDGEPREGVVIELTDAAGHLMRQVQSDFDGYVLFDTVPYGDYRLRIGAASAA
                     ALGVKADLGVVVRIDRANPSLRLGRIRLQSLVSAQVAVAP"
     gene            complement(2853..3680)
                     /locus_tag="VFV30_06685"
     CDS             complement(2853..3680)
                     /locus_tag="VFV30_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEX4847812.1"
                     /translation="MNFTASLYRLSLGLAALAAAAAAPARAQGDLLVAPTRVVINGGG
                     TAEVVLSNIGSQAATYRIGVELRRMEPDGDFADVTEAEANATEKAALEMLRYAPKRIT
                     LLPGQPQAIRLSARPAPELPDGEYRVHMSFRAVPPALSPEAAQEQVAAGQLAIRLTPV
                     YGITIPVFIRKGRLEAGATIANPRLGGNATSKWLELDMRRTGQRSVYGEILGKRGGEQ
                     VFQIKGVAIYPEVQGRTIKVPLTAEQAAKLTGPIRIEYRELPENGGQLIAEVTSTVR"
BASE COUNT          666 a         1322 c         1260 g          665 t
ORIGIN      
        1 gtcttcggtg taggcagtgc gggcaagcga atcgagcgac atggggtttc cctctggctg
       61 atttggattg gccagagatt tgacgctgcc ccgcgcggcg gtcaaccgct atttaatcga
      121 acgattaagg cgccaccgca acctgggcgc taaccagcga ttgcagccgg atccgaccga
      181 ggcgcagcga cggattggcc cggtcgatcc gcaccaccac gccaaggtcc gccttcacgc
      241 ccagtgccgc cgcgctcgct gctccgatcc gcaggcggta gtcgccatag gggactgtat
      301 cgaaaagcac gtagccatca aaatcgctct gcacctgccg catcaggtgg cctgcggcat
      361 ccgtcagttc gatcacaacc ccttcgcgcg gttccccgtc cgggcccagc agcagtgcct
      421 caagctcgcc ggttggcgcc agcggcaggc tgaccttggc catgacgccc ggacgcggca
      481 ccacaaccac gccctggccc ttgggctgga gcagcggatc gggcagcgtc ccggcatcga
      541 tcgagaccag cacgggcaca tagggtgtca ggccatcaat cacggcgcgg ccggccttgt
      601 tggtcggcgc gtcggtgtgg cggaagccgg cttcggccgc gacaccttcg accacgcctt
      661 caccggcctg gcggaagcca tcgccattct cgtcgcggaa cacctcgacc gccgcctggc
      721 cgctctcggc cagccgctga cgcgacatcc gccagccacc atcgaccgga tccggcccca
      781 ggctgaagcc cagggtcagg cccagggtca ggcggccgcg cgtatcgagc cggccttcgg
      841 tgcgcagggc aaagcgattg aactgacgga cccagccaag catgacctca tcgcggcgcg
      901 aggtggcatc gtgctcatag ccgagccgca gggtcgagct ctgtccgatc gcggtttccg
      961 caaccgcctg ggcgttctcc agcccgcgct gttggccatc caacccaaac cgcgccgccc
     1021 cacgcaggcg gaccggtccg atcgcggtgt tggcgatcag gttgagcttg gtgcccaggt
     1081 cttcgccaac cagcgccgtc gccggaccgc gcgcggtgcg gcgcgacagt tcggtggtca
     1141 gcgaggtccg cttgagctgc agcgccgaac gaattaacca ttcggtaacc tttgccccgg
     1201 ttttggtctg gaaatggcgg attccggtct cgaccggcag acgccaggac cccattttga
     1261 ccgaagtgct cagccgcagg ccatattcgc ggcgctgctg ggcctcgacg agctcgcttt
     1321 cgaagtcgcc atcgacccac agcgcctcgg cgtcatagcg aatcgcgcca aaacgcccga
     1381 gcgccttggc ctggaaggcc cggcccgcgc caagctgctg cgcgccgctc acttcgagca
     1441 gcatgcggcc cagcgcgcgc tggacagttc cttcgatgta atggcggcgg cggccggcga
     1501 acatcacgct ctgatattcg acgccggccg tggttcttcg gtcaattccg cgttccacgc
     1561 caacccccca gcgccagccg gtgttgggat cggcaaatgt cttcgagagg tcgatcagat
     1621 cgcgcccagc atcgaccacg ccggcccagt accaggtctt cccggcaggc aggctctcga
     1681 tcccgacggg cgcactgctg cgctcgcgct tgatctggcc ttgcgggcca tagagcacga
     1741 cttcgaagtc gttttcgccc cacagcagct cgatatcgtc aaaacggtag cgcccatcac
     1801 cgcgatcggc ctgataggcg cgaagctcgc cattgcgata gagttcggca tcccaaccgg
     1861 ccggcagcgt gccactcagc gatgtcaccc cgaaccggcc gggccggttg agcgggcgat
     1921 tggaaacaaa ggcgccgcga ccatagctgg tctgggcggt aagcgcgccg cgcagcgatt
     1981 ccacgtcgcc cagggcaacc tgcgtggcct tgagcggccc gagcagttca gccgaggggt
     2041 cgttgcggaa cagcttgagg cgcagcgagt cggcaccgtt atctgtgctg ccagcaaggc
     2101 gcgccgtata actgagcccg agcgcctcgc ccgaggcgag cgcctcgaac tgggcgcgcg
     2161 cgcccatgtt gcggctccac tgcccctgga tctggacatc gatcgagggc gtgcgccagt
     2221 tctggtaagg cgtttgcgct tgcggcagtt tggacaggtc gaattcggcg aatttcgggg
     2281 cgcgaagtcg cgcggcgcga cttttgcgtt cgatcgcctc gaggaacggc agcttgcgat
     2341 cggattccag caccaccgca agattggaaa ggtcggcgcg gaaccgcaca cccatccaac
     2401 cggacaaggc cttgagatcg acgcaccagc cttcaggcgt atcgtgaatc gcatcgcggg
     2461 cgatcgcacg gcttccgttc acgttttgta ccgtatcgga atcgcgatcg agggtgaagc
     2521 gctgatcctc cgcgaacagc cagccggttg cgcggcgcga cttcttgtca agtcgcaccg
     2581 gcaaatcgag cgcctggatg agatcggcaa agtcaacaca aacaccggcc ggggtctgat
     2641 agccgcgcag cggttcaccg aggcgatact ggccgctgcg cagttcgagc agcagcgaat
     2701 cgtcctcgtt cgcgctccat gcctgggcct gagcagatgc gcccgcacca acaacgccga
     2761 aagccgcccc gatcagggcg gccaggtggc gcagcggtgg gcgagtgccc ctcatgacgt
     2821 cagctttcgt ctttcattgt cggattgatg gatcagcgga ctgtcgaagt gacctcggcg
     2881 atcagctgtc cgccgttttc cggaagctcg cggtattcga tgcggatcgg cccagtcagc
     2941 ttggcggcct gctcggcggt cagcggcacc ttgatcgtgc ggccctggac ctcagggtag
     3001 atcgccacgc ccttgatctg gaacacctgc tcgccgccgc gcttgcctag gatctcgcca
     3061 tagaccgagc gctggccggt gcggcgcatg tcgagctcca gccacttgct ggtcgcgttg
     3121 ccgcccagcc gcgggttggc gatcgtcgcc ccggcttcga gccggccctt gcggataaag
     3181 accgggatgg tgattccgta gactggggtc aaccggatag cgagctggcc cgccgcgacc
     3241 tgttcctgcg ccgcttcggg cgaaagcgcg ggcggcacag cgcggaagct catgtgaaca
     3301 cggtactcgc cgtcgggcag ttccggcgcc ggccgtgcgc tcaggcggat cgcctggggc
     3361 tggccaggca gtagagtgat ccgcttgggc gcatagcgca gcatttcgag cgcggccttt
     3421 tcggtcgcgt tcgcttccgc ctcggtcacg tcagcaaaat cgccgtcggg ttccatccgg
     3481 cgcagttcga cgccgatacg ataggtggcg gcctggctgc cgatattgct cagcaccact
     3541 tcggccgtgc cgccgccatt tattaccacc cgggtcggcg ccaccagcag atcgccctgc
     3601 gccctggccg gagcggccgc agccgcggcc agggccgcca gcccaagcga caggcgataa
     3661 agcgaagcgg tgaagttcat ggctggaccc ctcgagacag gcttgccgaa atgccaacgg
     3721 cggcatcgcg gatttcccgc agggtctagg aaatcagggt taatatcccg ctaaggacga
     3781 ttgctttcgg acagcttgaa gaatgcacag ccaaagggcc gcacccgcgc gcggcggatg
     3841 cggcccatgg tctcccctgg ctgcggtaag acagccgggt tcgcagttct tactggtatt
     3901 cgaccgagac gct
//
LOCUS       DASUDV010000308         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1092415,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000308 DASUDV010000000
VERSION     DASUDV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092415"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VFV30_06690"
     CDS             complement(<1..1069)
                     /locus_tag="VFV30_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX4847813.1"
                     /translation="MQQLTPAEAALVASVDAAPMLDQVLAWSAVNTGTGNLPGLAQYV
                     AMLADAFATLPGEITLVDPAKVDAVAANGREIEVSYGKHLVLRVRPGANRRFLLTGHM
                     DTVFPADHSFQTTRWLDDETLNGPGVADMKGGIAVILAALKAFEGSPAASGVGYDVLL
                     NSDEETGSLSSAPLIAELAAGKVAALTYEPSALPDGTLAGARGGSGNYSLIITGKSAH
                     AGRNPQDGRNAIVAAAALALGLKALERPGLSINPARIDGGSANNVVPDHAVLRFNIRP
                     METELAEWFEQQIQQLIADVATEHGVAIHRHGGISRPPKPLDPPAERLFELVRDCGAA
                     LDQAEQFFGLFVDRLGRPADPA"
     gene            1331..3388
                     /locus_tag="VFV30_06695"
     CDS             1331..3388
                     /locus_tag="VFV30_06695"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX4847814.1"
                     /translation="MSDRVEKSGLQVDAALAAFIENEVVTPLGQDLNQFWAGFAALVE
                     RFVPVNRALLAKRNDLQVQIDAWHTARAGQPIDGGEYQAFLREIGYLVPEPAPFQVGT
                     QNVDPEIATMAGPQLVVPVLNARFLLNAANARWGSLYDAYYGTDALDAAPARPGGYDE
                     VRGAAVIEAVRKFLNSAIPGWEAALSGGACEHLVATKPGGYLFRHNGLHIEVVVDPAH
                     PVGKTDPLGIADVLLESALTTICDLEDSIAAVDAEDKLNAYRNWLGVIRGDLADTFEK
                     GGKTMTRTLNGDREWGDVTLSGRSLLFVRNVGHLMTNPAILLADGSEIPEGIMDAVIT
                     SAIGAHDVRGLSRYRNSRHGSIYIVKPKQHGPEEVAFTNDLFGAVEDLLDLPRHTLKV
                     GVMDEERRTSANLAACIHAVKDRVVFINTGFLDRTGDEIHTSMRAGPMIRKGDMKKSG
                     WIAAYEARNVQIGLACGLSGKAQIGKGMWAAPDLMGEMMVQKIGHPKSGANTAWVPSP
                     TAATLHAMHYHAVDVFAVQKELAGQPTPPLVALLSVPLAEGTNWSEDEIREELDNNAQ
                     GLLGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQHMANWLLHGVATRDQVMDS
                     LRRMAAKVDAQNAGDPAYEPLVGNEDAPAFRAAMDLVFKGVEQPSGYTEPLLHAWRRR
                     KKG"
     gene            complement(3385..>3900)
                     /locus_tag="VFV30_06700"
     CDS             complement(3385..>3900)
                     /locus_tag="VFV30_06700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEX4847815.1"
                     /translation="TSVAHGIEGPFHIVANLPYNVGTALFTGWLGGEHWPPQWASLTL
                     MFQLEVAQRIIAEAGSDAYGRLAVLAQWRSEARIAMKVHRSAFTPPPKVMSAIVHVTP
                     GQMPEGISARKLERLTEAAFGQRRKMLRQSLKGLPGALEALERLGIDPARRAETLSVA
                     EFVSVARELSA"
BASE COUNT          664 a         1284 c         1297 g          655 t
ORIGIN      
        1 cggcgggatc agccggccgc ccaagccgat cgacgaaaag gccgaaaaat tgttcggcct
       61 ggtcgagcgc ggcgccacaa tcgcgaacca gttcgaacag gcgttcggcc ggcggatcga
      121 gcggcttggg cgggcggctg attccgccat gacggtggat tgccacgcca tgctcggtcg
      181 cgacatcggc gatcagttgc tgaatctgct gctcgaacca ttccgccagc tcggtctcca
      241 tcgggcggat gttgaagcgc agcacggcgt gatcgggcac cacattgttg gcgctgccgc
      301 cgtcgatcct ggcggggttg atgctgaggc cagggcgctc cagtgccttg aggcccaggg
      361 ccagggcagc cgcagcaacg atcgcgttgc gcccgtcctg cgggttgcgc ccggcatggg
      421 ccgacttgcc ggtgatgatc aggctgtaat tcccgctacc accgcgggcg ccggccaggg
      481 tgccgtcggg cagggccgac ggttcatagg tcagcgccgc cactttcccc gccgccagtt
      541 cggcgatcag cggggcggag gagaggctgc cggtttcctc gtccgaattg agcagcacgt
      601 catagcccac gcccgaggca gcgggactgc cctcgaacgc cttcagcgcg gcgaggatca
      661 ccgcaatgcc gcccttcatg tccgcaacac cggggccgtt cagggtctcg tcgtcgagcc
      721 agcgggtcgt ctggaacgaa tggtcggcgg ggaagaccgt gtccatgtgc ccggtcagca
      781 ggaagcggcg gttggcaccg ggccgcaccc gcagcaccag gtgtttgccg tatgacactt
      841 cgatctcgcg cccgttggcg gcgacagcgt caaccttggc cgggtcaacc agcgtgatct
      901 cccccggcag cgtggcgaac gcatcggcca gcatggcaac atactgcgcc aaccccggca
      961 ggttgcccgt gccggtgttg accgcgctcc aggccagcac ctgatccagc attggcgcag
     1021 catcgacgct ggcaaccagc gcggcttcgg cgggggtaag ttgttgcatg tgttaccacc
     1081 tctagccgag gcgcgtgggg gttccaaccc ccggcacaaa agtccccctc ccgcttgcgg
     1141 gaggggctag gggtgggcct ggttaaagca agcagcgggc ccacccccga cccctcccgc
     1201 aagcgggagg ggagacagtt gccatccctt cgcaactgcg gcataggcgc gtgacttcct
     1261 ccccgggatc gttgatgaac cacttgccag cacaccttgg tgctgcgcga ttcaatgcgt
     1321 gcgaggtaga atgagcgatc gggttgagaa gtcgggcctg caagtcgatg cggcgctggc
     1381 cgcttttatc gagaacgaag ttgtcacccc gctcgggcag gacttgaacc agttctgggc
     1441 aggtttcgcc gcgcttgtag agcggttcgt gccggtcaac cgcgcgctgc tagccaagcg
     1501 caatgacctg caggtgcaga tcgacgcctg gcacaccgcc cgcgccgggc agccgatcga
     1561 cggcggcgaa taccaggctt tcctgcgcga aatcggctac ctggttcccg agcccgcccc
     1621 gttccaggtc ggcacgcaga acgtcgatcc cgagatcgcg accatggccg ggccgcagct
     1681 ggtcgtgccg gtgctcaatg cccggttcct gctcaatgcc gccaatgccc gctggggtag
     1741 cctttacgat gcctactacg gcaccgatgc gctcgatgcc gcgccggccc gtccgggtgg
     1801 ctatgacgag gtgcgcggtg cagcggtgat cgaagcggtg cgcaagttcc tgaacagcgc
     1861 gatccccggc tgggaagcgg cgctgagcgg tggtgcctgc gagcacctcg ttgctaccaa
     1921 acccggcggc tatctcttcc gccacaacgg cctgcacatc gaagtggtgg tcgatccggc
     1981 gcatccggtc ggcaagaccg acccgctcgg cattgccgat gtcctgctcg aatccgcgct
     2041 gaccacgatc tgcgacctcg aggattcgat tgcggcggtg gatgccgaag acaagctcaa
     2101 cgcctatcgc aactggctcg gcgtgatccg cggcgatctc gccgatacct ttgaaaaggg
     2161 cgggaaaacc atgacccgca cgctcaacgg cgatcgcgaa tggggcgatg tgaccctgtc
     2221 cgggcgcagc ctgctgttcg tccgcaatgt cggccacctg atgaccaacc cggcgatcct
     2281 gctcgccgat ggcagcgaga tccccgaagg gatcatggac gcggtgatca ccagcgcgat
     2341 cggggcccac gatgtgcgcg gcctttcgcg ttatcgcaac agccgccatg gttcgatcta
     2401 tatcgtcaag cccaagcagc acgggcctga agaagtagcc ttcaccaatg atttgttcgg
     2461 cgctgttgag gacctgcttg atcttccgcg ccacaccctg aaggtcgggg tgatggacga
     2521 ggagcgccgc acttcggcca atcttgccgc ctgcatccac gcggtgaagg accgggtggt
     2581 gttcatcaac accggcttcc tcgatcgcac cggggatgaa atccacacct cgatgcgcgc
     2641 cgggccgatg atccgcaagg gcgacatgaa gaagagcggc tggatcgccg cctacgaagc
     2701 gcgcaacgtc cagatcggcc tggcctgcgg gctttccggc aaggcgcaga tcggcaaggg
     2761 gatgtgggcc gcgcccgacc tgatgggcga gatgatggtc cagaagatcg gccacccgaa
     2821 aagcggcgca aacaccgcct gggtcccttc gcccactgcc gccacgctgc acgcgatgca
     2881 ttatcacgcg gtcgatgtct ttgccgtcca gaaggagctg gctggccagc ccaccccgcc
     2941 gcttgtcgcg ctgctgagcg tgccgctcgc cgaaggcacc aactggtcgg aggacgagat
     3001 ccgcgaggaa ctggacaata acgcgcaggg gctgctcggc tatgtcgtgc gctggatcga
     3061 tcagggcgtg ggctgttcga aggtgcccga catcaacgac gtgggcctga tggaagaccg
     3121 cgcgaccttg cgcatttcca gccagcacat ggccaactgg ctgctccacg gggttgctac
     3181 ccgcgaccag gtgatggact ccttgcgccg catggccgcc aaggtcgatg cccagaatgc
     3241 cggcgatccg gcctatgagc ccttggtggg caacgaggat gcaccggcct tccgcgccgc
     3301 gatggacctg gtgttcaagg gcgtggaaca gcccagcggc tacaccgaac cgctgctcca
     3361 cgcctggcgc aggcgcaaga agggttaggc tgacagctcc cgcgccacgc tcacgaattc
     3421 cgccacgctc agcgtttcgg cgcggcgggc cgggtcgatg cccagcctct ccagcgcctc
     3481 gagcgcgccg ggcaggccct tgaggctctg gcgcagcatt ttgcggcgct ggccgaaggc
     3541 ggcttcggtc aggcgttcaa gtttgcgggc ggagatgccc tcgggcatct gtccgggggt
     3601 gacgtggacg atcgcgctca tcaccttggg cggcggggtg aaggcgctgc ggtgaacttt
     3661 catcgcaatc ctcgcctcgc tgcgccactg cgccagcacc gccagccggc cataggcatc
     3721 gctgcccgct tcggcgatga tccgctgcgc cacttcgagc tggaacatca gcgtcaggct
     3781 ggcccattgt ggcggccagt gctcaccccc cagccagccg gtgaacagcg cggtgccgac
     3841 gttgtagggc aggttggcga cgatgtggaa cgggccctcg atgccatgtg caacgctggt
//
LOCUS       DASUDV010000309         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1445569,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000309 DASUDV010000000
VERSION     DASUDV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445569"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFV30_06705"
     CDS             <1..957
                     /locus_tag="VFV30_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847816.1"
                     /translation="SGQTVLVSVTNGGNPALRKETRRDWKFGATWQLPVLSGSSAVIE
                     YIRNRSDDVSANFPLLTPQIEAAFPGRITRDGTGKLVAIDSRPVTFAQQTSSRIRWGF
                     SLSDTLGKAAPGGGGGGMAMRPGGPGPGQRPPGAGGGRPGGGGGGRMMAMMMGGGGQG
                     RWSLGIYHTVQLDSRVLIAPGVPLLDLLDGDSLSASGTPRHAIEFNGGLFHKGKGMFF
                     QGTWNAPTTLVASGVPGSSDLRFGAVTKANVNFFVELGDQGKLAEKSPLFKGLRLSLR
                     IENLFDSRQKVTDANGLVPLGYQADYLDPRGRVIEFELRKMF"
     gene            complement(986..1417)
                     /locus_tag="VFV30_06710"
     CDS             complement(986..1417)
                     /locus_tag="VFV30_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4847817.1"
                     /translation="MIGMEILKPLAVLAGWTMVMWLWMYVTRIPAMNAAKIDTKNLTG
                     GTGKDLEAVLPGKVQWIAHNYNHLHEAPTVFYAVALALALVGQGDGLNATLAWAYVGL
                     RIAHSLVQAVWNRVMVRFTLFALSSLALMALTLHLLIAVFH"
     gene            complement(1421..1858)
                     /locus_tag="VFV30_06715"
     CDS             complement(1421..1858)
                     /locus_tag="VFV30_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4847818.1"
                     /translation="MQAQMLAPAAVLVVWTLIMLTWLAATRLPPLFKQPGGLGSAKRG
                     GRGQDLEGVLPDSVQWKSHNYTHLLEQPTLFYAASVIIAIMGAEGHDVLLAWSYVVLR
                     VVHSVWQAVVNTVPVRFTLFILSTVCLLLLAIRAVELTLMAGA"
     gene            complement(1932..>2298)
                     /locus_tag="VFV30_06720"
     CDS             complement(1932..>2298)
                     /locus_tag="VFV30_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4847819.1"
                     /translation="PVEPEGWDDVRLSLEQGAIVGVPDMKFPTQCDALQTPATWPINF
                     AVLKKRVARGYAVSAAEVRAAQRWAFANLHLVVEPGGAAALAAVLAGKVPVDGRTAVI
                     LSGGNTDPEAFANVLLGSD"
BASE COUNT          412 a          755 c          749 g          382 t
ORIGIN      
        1 agtggccaga ccgtgctggt cagcgtgacc aatggcggca atccggcgct gcgcaaggaa
       61 acccggcgcg actggaaatt cggcgcaacc tggcaattgc cggtgttgtc gggttcaagc
      121 gcagtgatcg aatatatacg caaccgctcg gatgatgtca gcgccaactt tccgctgctg
      181 accccgcaga tcgaggcggc gtttcccggc cggatcaccc gcgatggcac cggcaaactg
      241 gtggcgatcg acagccgccc ggtaaccttc gcccagcaga ccagctcgcg catccgctgg
      301 ggcttctcgc tgagcgacac gctgggcaag gcggcacccg gcggcggtgg cggcggcatg
      361 gcgatgcgcc ccggcggtcc cgggcccggc cagcgccctc ccggtgcggg cggcgggcgt
      421 cccggcggcg gtggtggcgg ccggatgatg gcaatgatga tgggcggtgg tggtcagggc
      481 cgctggagcc ttggcatcta tcacacagtg cagctcgaca gccgggtgct gatcgcaccg
      541 ggggtacctc tgctcgatct gcttgatggc gattcgctct ccgccagtgg caccccgcgc
      601 cacgcgatcg aattcaatgg tgggctgttc cacaagggca aggggatgtt ctttcagggc
      661 acctggaacg cgccgaccac cctggttgcc agcggggttc cgggcagcag cgatctgcgc
      721 tttggcgccg tgaccaaggc gaatgtcaac ttcttcgtgg aactgggcga tcagggcaag
      781 ctggccgaaa agagcccgct gttcaaagga ctgcgcctgt cgctgcggat cgagaacctg
      841 ttcgattcgc gccagaaggt taccgatgca aacggcctgg tgccgctggg ctaccaggcc
      901 gattaccttg atccacgcgg ccgcgtgatc gaatttgaac tcaggaagat gttctagggg
      961 gcagtccagc gaccgccccc gttgctcagt gaaacactgc gatcagcagg tgcagggtca
     1021 gcgccatcag cgccaggctg gacagcgcaa acagcgtgaa gcgcaccatc acccggttcc
     1081 aaaccgcctg gacgagcgag tgcgcgatcc tgaggccgac ataggcccag gccagggtgg
     1141 cgttcagccc atcgccctgg ccgaccagcg ccagtgccag cgctaccgca tagaacacgg
     1201 tcggcgcttc atgcaggtgg ttgtagttgt gggcaatcca ctggaccttg cccggcagca
     1261 ccgcttccag gtccttgccg gtcccgcccg tcaggttctt ggtatcgatc ttcgcggcgt
     1321 tcatcgccgg gatgcgggtg acgtacatcc acagccacat caccatcgtc cagccggcca
     1381 gtaccgccag cggcttcaag atttccattc cgatcatcgt tcaggctcct gccatgaggg
     1441 taagttcgac cgcgcggatc gccagcagca gcaggcacac ggtcgaaagg ataaacagcg
     1501 tgaagcgcac cggcacggtg ttgaccaccg cctgccacac cgagtggacc acccgcagca
     1561 cgacgtagga ccaggccagc agcacatcgt ggccttccgc gcccatgatc gcgatgatca
     1621 cgctggctgc gtagaacagc gtcggctgtt cgagcagatg ggtgtagttg tgcgatttcc
     1681 actgcacgct gtcgggcagg accccttcga ggtcctggcc acggccaccg cgcttggccg
     1741 accccagccc gccgggctgc ttgaacagcg gcggcaggcg ggttgccgcc agccaggtaa
     1801 gcatgatgag cgtccacacc accagcacgg cggcgggtgc gagcatttgt gcctgcatcg
     1861 atttttctcc cccgggattg ttgacccagg ggcgggaagg tgggtcaggc ccgaacgatt
     1921 gtcaaatgca actaatcgct gccaagcagc acgttggcaa aggcctcggg atcggtgttg
     1981 ccgcccgaca ggatcaccgc ggtacggcca tcgaccggta ccttgcctgc cagcaccgcc
     2041 gccagcgccg ccgcaccgcc cggttcgacc accaggtgca gattggcaaa ggcccagcgc
     2101 tgcgccgcgc gcacctcggc agcggaaacc gcgtagccgc gcgcaacccg cttcttgagc
     2161 accgcgaagt tgatcggcca ggtggcgggg gtctgcagcg cgtcgcactg ggtcgggaac
     2221 ttcatgtccg gcacgccaac gatcgcgccc tgttccaggc tcaggcgaac atcatcccag
     2281 ccttcgggtt cgaccggc
//
LOCUS       DASUDV010000310         9665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1578332,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000310 DASUDV010000000
VERSION     DASUDV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578332"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFV30_06725"
     CDS             <1..733
                     /locus_tag="VFV30_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4847820.1"
                     /translation="GQAALVEAARFDEFNRRIAFVMHDIKNLASQMALLARNAESHAD
                     NPDFRADMLVTLRNSTDKLNALIARLSRYGTANIGAPRPVRADLIAKDVVERLAKAHP
                     VTLVESADFSVAADPDSLEQVLTHLIQNAIDASAPDAPVFVAVSNDGLNGAFEVVDSG
                     TGMSPEFVRSRLYKPFESTKQGGFGIGAYEARELVKAMRGRIEVESREGLGTRFIVRL
                     PLAEAAALYENFNVNTPGQTGKKVA"
     gene            734..2098
                     /gene="prsR"
                     /locus_tag="VFV30_06730"
     CDS             734..2098
                     /gene="prsR"
                     /locus_tag="VFV30_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX4847821.1"
                     /translation="MSESKPKLLIVEDDAGLQAQLKWAYPDFEVIQATDRAGAIAALR
                     SEEPAVVTLDLGLPPDPDGTSEGFAVLDEIMALKPDTKVIVASGHGARESALQAIAKG
                     AYDFYQKPVDIEALGLIVRRALQLHRIEAENRQLASKSGEEHRVLGRLITAAPEMVKV
                     ARTIERVANTKVSVMLLGASGTGKELLARGLHEASDRAGGAFVAINCAAIPENLLESE
                     LFGHEKGAFTGAVKTTEGKIEMADGGTLFLDEVGDIPLPLQVKLLRFLQERVIERIGS
                     RKSIAVDTRIVCATHQNLEAMIAEGRFREDLFYRLAEIVVKIPSLAERPGDPALVAKA
                     FLARFAKEMNPAVKGFAPDALTAIDAWHWPGNVRELENRVKRAVIMAEGKLIGAADLD
                     LAEPDEDEGTVLNLKCAREIADRRVIRHALARSEGNISNTAKLLGISRPTLYDLLKQY
                     DLQA"
     gene            2103..3854
                     /locus_tag="VFV30_06735"
     CDS             2103..3854
                     /locus_tag="VFV30_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4847822.1"
                     /translation="MRRLTLALALAALSAPVWAVPEAAQESLDKARAALSRGDGIAAE
                     AELQRTLGAGATRADIAADMGEAYIQQGERKKAREWLGPAQFAKGGEARGWRLLGLLE
                     RLDGNLPAAGRALDRAIAAGPKDPLVWVEVGRLRYQGGEQLQAIEAAEEALKHGPEEP
                     RALEFMAQLVRDSAGDAAALPFYERALEVTPDDLSLLGGYAAALGEMGRGREMLAVTR
                     KMIGIDPGAPLPFYLQAVLAARAGKTELARAMLNRCGNRLDGMPAAKLLLGALELEAG
                     NANLAVQHLSRLADQQGANPWVQLLLARALYEAGDTKQLFARYGAQAGRGDASPYLLT
                     VLARAYEDMGDRAAAAPLLERAAAALPAAVMAIPDGSGASVRGLLAAGNVAGAVRVGS
                     AAVDQRPGSSEALAQLGDAELLAGQPGRAFDRYSLAARVRFTDQLALRSAIALEQGGR
                     ANQVPALAAGYLGIYPSSRLMARLAANLAIGAGDWAQARLILENLRLRGGSRDARLMA
                     DLGLAQLRTGDAEAALNSAARARAIAPASSFAVLVHALALAEREETRDQARQLVAQAA
                     KIDPKHPLLAEARRKLR"
     gene            3931..4989
                     /locus_tag="VFV30_06740"
     CDS             3931..4989
                     /locus_tag="VFV30_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4847823.1"
                     /translation="MAGVLQGVTVIELAGIGPGPFCGMMLADHGARVIRIERPGTSGR
                     FGDGGNRDILNRNRERIELDLKDPAAIEQLKELVKTADALIEGYRPGVIERMGIGPDV
                     LLGINPQLVIGRMTGWGQDGPMAPLAGHDINYIALSGALHSYGRKGEKPIFPVNAVGD
                     FGGGGMMLAFGVVAAILHARSGGTGQVVDCAMVDGAAILSAMTYTFLGNGQWRDERGV
                     NLLDSGTHFYDTYETSDGKYISLGSIEPQFYAILMEKTGLAGDPDFAQQMNPMKWDEL
                     KERMTALILTKTRDEWCAIMDGTDICFAPVLSLKEAPQHPHNVARATFVEDGGMVQPA
                     PAPRFSATPAPAPKLAGR"
     gene            4986..5942
                     /locus_tag="VFV30_06745"
     CDS             4986..5942
                     /locus_tag="VFV30_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX4847824.1"
                     /translation="MSLLNRIDPMVRLLVLAIALALVVPVTGGAREIAQFVANAAVFL
                     LFFLNGVRLPRHEVAQGLSNHRLLWPLVAWCFGGMALAGWGLWQAGQGWMPPLIALGF
                     LYLGCLPSTVQSATAYSSLAGGNVASSVVAAALLNLLGVFVTAPLFSLLGGGGAAEFH
                     GDGLLKVVMILLVPFMLGQVMQGRLSGWVRDNKDLTTWFDRGSIAIAVYVAFSGAVEQ
                     RFWQQVDPAGWGWLLGGTALFLVIGHGGAWLLGGMLRLDRGNRVSMLFAGAQKSIAMG
                     APLAAVLFAPAVAGVVLLPILLYHLAQLVLAAPIAARLNPPG"
     gene            complement(5926..6996)
                     /locus_tag="VFV30_06750"
     CDS             complement(5926..6996)
                     /locus_tag="VFV30_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEX4847825.1"
                     /translation="MLMKFYKGSILFTVVCLALGVWYGWSQTHDLGATASMLWIIFVL
                     SILEVSLSFDNAVVNAAVLKDMDEVWQRRFLTWGMVIAVFGMRIVFPLAIVAVAAGLG
                     PMDAINLSLNSPEEYEAIVSSAHVGIAGFGGAFLAMVGLSFFFDGEKDVHWIEWIEHK
                     LSVVSNIKAAEIALLLLVLYGISLLLPPADALTFMVSGTLGLVTFIAVEAISTILEMR
                     EEAMKLQGAVVRSGLGGFLYLNILDASFSFDGVIGAFALSNNMVVIALGLSIGAMFVR
                     SMTIHLVKQGTLAEYRYLEHGAFWAIIALGGIMLFSAVMHIPETVTGLIGAVLIGLSL
                     WWSIRHNKRNPDREIEDAIRAD"
     gene            complement(7007..7876)
                     /gene="panB"
                     /locus_tag="VFV30_06755"
     CDS             complement(7007..7876)
                     /gene="panB"
                     /locus_tag="VFV30_06755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX4847826.1"
                     /translation="MSTTYQLDTATSRAHPTPAPLRRLTIPTIRQRKQDGVTAEPVVM
                     LTAYTARQAQLLDVHCDLLLCGDSLGQVIYGLPSSVPVTLEMMAAHGAAVVRGSYHAA
                     VVVDLPFGTYEGSKEQAFTSAARLLKETGCDAIKLEGGAAMAETVDFLTRRGIPVMGH
                     VGLTPQAVNLLGGYGARGRSEAEAEKIVSDAKALDQAGAFAIVIEGVVEPVAIAATKA
                     VACPTIGIGASAQCDGQVLVTEDMLGMFERVPRFVKRYEEIAETISAAAARYAAEVRD
                     RSFPGIEQTYQPK"
     gene            8019..9575
                     /locus_tag="VFV30_06760"
     CDS             8019..9575
                     /locus_tag="VFV30_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4847827.1"
                     /translation="MFGRVKPLDAILATAEKKSLHRSLGAFQLTMLGIGAIIGTGIFV
                     LTAEAAQKAGPGMMLSFIMAGFVCAVAALCYAEMASMVPVSGSAYTYSYASMGEIVAW
                     IVGWALILEYAIAASAVSVGWSNYFVGLVQSGLGMELPTFFTKGAFAAVGGGINIPAI
                     FVALLVTALLVIGTKESATFNAVLVVIKVVALTAFIILAVPVMKMENFEPFSPLGFGG
                     VSAAAASIFFAYVGFDAVSTAAEETKNPQRNMPIGLIGSLVICTIFYLLVAAGAIGAV
                     GAQPVMGAAGEALATGSKELQAQCAALTSAGTVPLVCSDEALAHVLREIGHVQVGNLI
                     GLAAFLALPSVVLMMLFGQTRIFFVMARDGLLPEGLAKVHPKFKTPHVVTIITGIFVT
                     MAAAFFPVGQLADISNSGTLFAFMTVAIGVMILRRTAPDRHRPFRTPFVWLVGPAAVA
                     GCAYLFFNLSGYTELMFLGWAAIGLVVYFAYGRRKSHVGRGLVEVHEQDADAPPQPVP
                     PLPGGIDIND"
BASE COUNT         1537 a         3191 c         3357 g         1580 t
ORIGIN      
        1 cgggcaggcg gcgctggtcg aagcggcccg gttcgatgaa ttcaaccgcc ggatcgcctt
       61 cgtgatgcac gacatcaaga acctggcgag ccagatggcg ctgctggcgc gcaacgccga
      121 aagccatgcc gacaatccgg acttccgggc cgacatgctg gtcacgctgc gcaattcgac
      181 tgacaagctc aacgccctga tcgcccggct ctcgcgctat ggcacggcca acatcggcgc
      241 cccgcgtccg gtgcgggccg atctgatcgc gaaggacgtg gtcgagcggc tggccaaggc
      301 gcacccggtt accctggtcg aatccgcgga cttcagcgtc gcggccgatc cagattcgct
      361 ggagcaggtg ctgacccatc tgatccagaa cgcgatcgat gccagtgcgc ccgatgcccc
      421 ggtcttcgtg gcggtcagca acgacgggct caacggcgcg ttcgaagtgg tcgattccgg
      481 cacagggatg agccccgaat tcgtccgcag ccggctctac aagccgtttg aatcgaccaa
      541 gcagggcggg ttcgggatcg gggcctacga ggcgcgcgag ctggtcaagg ccatgcgcgg
      601 ccggatcgag gtggaaagcc gcgaggggct cggcacccgc ttcatcgtcc gcctgccgct
      661 ggccgaggcc gccgcgcttt acgagaattt caacgtcaac acgccgggcc aaaccggcaa
      721 gaaggtcgcc tgaatgtctg agagcaaacc caaactgctg attgtcgagg atgacgccgg
      781 gctgcaggcg cagctcaagt gggcctatcc cgacttcgag gtgatccagg ccaccgaccg
      841 ggccggtgcg atcgcggcgc tgcgcagcga ggagccggcc gtagtgacgc tcgacctggg
      901 gctgccgccc gatcccgacg gcactagcga gggctttgcc gtgctggacg agatcatggc
      961 gctgaagccc gataccaagg tgatcgttgc ctcgggccac ggcgcgcgcg aatccgcgct
     1021 ccaggccatc gccaagggcg cctatgattt ctaccagaag ccggtcgata tcgaggcgct
     1081 gggcctgatc gtccgccgcg cgctgcaact gcaccggatc gaggcggaga accgccagct
     1141 ggccagcaag agcggcgagg agcaccgcgt gctcggccgc ctgatcaccg ccgcgccgga
     1201 aatggtgaag gtggcccgca cgatcgagcg ggtggccaac accaaggttt cggtcatgct
     1261 gctgggcgcc agcggcaccg gcaaggaatt gctggcgcgg ggcctgcacg aagcgagcga
     1321 ccgtgcaggc ggcgccttcg tcgcgatcaa ctgcgccgcg atccccgaaa acctcctcga
     1381 aagcgagctg ttcgggcacg agaagggggc ctttaccggg gcggtgaaga ccaccgaggg
     1441 caagatcgag atggccgacg gcggcacgct gttcctggac gaagtgggcg acattccgct
     1501 gccgctccag gtcaagctgc tgcgctttct gcaggagcgg gtgatcgagc ggatcggatc
     1561 gcgcaagtcg atcgcggtcg acacccggat cgtctgcgcc actcaccaga acctggaggc
     1621 gatgattgcc gaaggccggt tccgcgaaga cctgttctac cgtctggccg aaatcgtggt
     1681 gaagatcccc agcctggccg aacggcccgg tgatccggcg ctggtcgcca aggcgttcct
     1741 ggcccgcttt gccaaggaaa tgaacccggc ggtcaagggc tttgcccccg acgccttgac
     1801 cgcgatcgat gcctggcact ggccgggcaa cgtccgcgag ctggaaaacc gcgtgaagcg
     1861 cgcggtgatc atggccgagg gcaagctgat cggcgcggcc gatctggacc tggccgagcc
     1921 cgacgaggac gagggcacgg tgctgaacct gaaatgcgcg cgcgagatcg ccgatcggcg
     1981 ggtgatccgc cacgcgctgg cccgcagcga aggcaatatc tcgaacaccg ccaagctgct
     2041 cgggatcagc cgtccgacgc tttacgatct gctcaagcag tacgacctgc aggcctgagg
     2101 caatgcggcg gctgaccctt gccctggccc tggcggcgct ttccgcgccg gtctgggccg
     2161 tgcccgaagc ggcgcaggag agcctggaca aggcccgcgc ggcgctttcg cgcggggacg
     2221 ggatcgccgc cgaggccgag ctgcagcgga ctctgggtgc cggggcgacc cgcgccgaca
     2281 tcgccgccga tatgggcgag gcctatatcc agcagggcga gcgcaagaag gcccgcgaat
     2341 ggctaggccc ggcccagttc gccaagggcg gggaagcacg cggctggcgc ctgctcggtt
     2401 tgctcgaacg gctggacggc aatctgccgg cggccggacg cgcgcttgac cgggcgattg
     2461 cggcggggcc caaggatccg ctggtctggg tcgaggtcgg gcggctgcgc tatcagggcg
     2521 gcgaacagct tcaggcgatc gaagccgccg aagaagcgct caagcacggc cccgaagagc
     2581 cgcgcgcgct ggaattcatg gcgcagctgg tgcgggattc ggccggggac gccgccgcat
     2641 tgccgttcta cgaacgggcg ctggaggtca ctccggacga tctgtcgctg ctgggcggct
     2701 atgccgccgc actgggcgaa atgggccggg gccgcgagat gctggccgtc acccgcaaga
     2761 tgatcgggat cgatcccggc gcgccgctgc cgttctatct gcaggcggtg ctggcagccc
     2821 gggccggaaa gaccgagctg gcccgcgcga tgctcaaccg ctgcggcaac cggctggacg
     2881 gcatgccggc ggccaagctg ctgctgggcg cgctggaact ggaagccggc aacgccaacc
     2941 tggccgtgca gcacctgtcc cgcctggccg atcagcaggg cgccaacccc tgggtgcagc
     3001 tgctgctggc ccgggcgctt tacgaagcgg gcgacaccaa gcagctgttc gcgcgctatg
     3061 gcgcccaggc ggggcggggc gatgcctcac cctatctgct aaccgttctg gcccgcgcct
     3121 atgaggacat gggcgaccgg gctgctgccg ctccgctgct ggaacgggcc gctgccgcgc
     3181 tgcccgcggc ggtgatggcg atccccgatg gcagcggtgc cagcgtgcgc ggcctgctgg
     3241 ctgcgggcaa tgtcgccggt gcggtccggg tgggaagcgc cgccgtggac cagcgccccg
     3301 gctcgtccga agccctggcc cagcttggcg atgcggagct gctggccggg caaccgggcc
     3361 gggccttcga tcgctacagc ctggccgcgc gggtgcgctt taccgaccag ctggcgctgc
     3421 gctcggccat tgcgctggaa cagggcggac gcgccaacca ggtgccggcc ctggcggcgg
     3481 gctacctggg gatctacccc tcgagccggc tgatggcgcg gctggcggcc aacctggcaa
     3541 tcggcgcggg cgactgggcg caggcccggc tgatcctgga aaacctgcgg ctgcgcggcg
     3601 gcagccggga tgcgcggctg atggccgatc tgggcctggc ccagttgcgg accggcgatg
     3661 ccgaagccgc cctgaacagc gcggcgcggg cccgggcgat cgccccggcg agttcctttg
     3721 ccgtgctggt ccacgccctg gccctggccg agcgcgagga gacccgcgac caggcccggc
     3781 agctggtggc ccaggcggcc aagatcgatc ccaaacaccc cctgctggcc gaggcccgcc
     3841 gcaaattgcg ctaagccccc tcgacaagcc gcccgcgcgc cccataaagg cgaatttcat
     3901 cgcgcgatta aatttggcgg agaggatggc atggctggtg tacttcaggg cgtaaccgtt
     3961 atcgaactgg ccgggatcgg cccggggccg ttctgcggca tgatgctggc cgaccacggc
     4021 gcgcgggtga tccggatcga acggcccggc accagcggcc ggttcggcga cggcggcaac
     4081 cgcgacatcc tcaaccgcaa ccgcgagcgg atcgagcttg acctcaagga cccggccgcg
     4141 atcgagcagc tcaaggagct ggtgaagacc gccgacgcgt tgatcgaagg ctatcgcccg
     4201 ggcgtgatcg agcggatggg cattggcccc gacgtgctgc tggggatcaa tccccagctg
     4261 gtgatcggcc gcatgaccgg ctggggccag gacgggccga tggccccgct ggccgggcac
     4321 gacatcaact acatcgccct ttcgggcgcg ctccacagct atggccgcaa aggcgaaaag
     4381 ccgatcttcc cggtcaacgc cgtgggcgat ttcggcggcg gcgggatgat gctggccttc
     4441 ggcgtggtcg cggcgatcct gcacgcgcgt tcgggcggga ccgggcaggt ggtcgactgc
     4501 gcgatggtcg atggtgcggc gatcctttcg gccatgacct acaccttcct tggcaacggc
     4561 cagtggcggg atgagcgcgg ggtcaacctg ctcgacagcg gcacgcactt ctacgacacc
     4621 tacgagacca gcgacggcaa atacatctcg ctcggctcga tcgagccgca gttctacgcg
     4681 atcctgatgg agaagacggg cctcgccggc gatcccgatt tcgcccagca gatgaacccg
     4741 atgaagtggg acgagctgaa ggagcggatg accgcgctga tcctgaccaa gacccgcgac
     4801 gaatggtgcg cgatcatgga cgggaccgac atctgtttcg ccccggtgct gagcctgaag
     4861 gaagcgcccc agcatccgca caacgtggcc cgcgccacct tcgtcgagga cggcggcatg
     4921 gttcagccgg ccccggcacc gcgcttctcc gccaccccgg ctccggcgcc gaagctggcg
     4981 gggcgttgag cctcctgaac cgcatcgacc ccatggtgcg gctgctggtg ctggcgattg
     5041 cgctggcgct ggtggtgccg gtgacgggcg gcgcgcgcga aattgcgcag ttcgtcgcca
     5101 acgccgcggt gttcctgctg tttttcctga acggtgtgcg cctgccccgg catgaggtgg
     5161 cgcagggcct gtccaaccac cggctgctct ggccgctggt cgcctggtgc tttggcggca
     5221 tggcgctggc cgggtggggg ctgtggcagg ccgggcaggg ctggatgccg ccgctgatcg
     5281 cgctggggtt cctctatctc ggctgcctgc cgtcgacggt ccagtcggcc acggcctatt
     5341 cctcgctggc cgggggcaat gtcgccagct cggtcgtggc ggccgcgctg ctcaacctgc
     5401 tcggcgtatt cgtcaccgcg ccgctgttct cgctgcttgg cggcggcggt gcggcggagt
     5461 ttcatggcga cgggctgctg aaagtcgtga tgatcctgct ggtgcccttc atgctggggc
     5521 aggtgatgca ggggcgcctg tcgggctggg tccgggacaa caaggatctg accacctggt
     5581 tcgatcgcgg ctcgatcgcc attgcggtct atgtcgcctt ttccggcgcg gtcgagcagc
     5641 ggttctggca gcaggtcgat ccggccggtt ggggctggct gctgggcggc accgcgctgt
     5701 tcctggtgat tggccacggc ggggcctggc tgctgggcgg catgctgcgg ctggaccggg
     5761 ggaaccgcgt ttccatgctg ttcgccggcg cgcagaagag catcgcgatg ggcgcgccgc
     5821 tggccgcggt gcttttcgcg ccggccgtgg cgggtgtcgt gctgctgccg atcctgctct
     5881 atcacctggc gcagctggtg ctggcggcgc caatcgccgc ccggctcaat ccgcccggat
     5941 agcgtcctcg atctcgcggt cggggttgcg cttgttgtgg cggatcgacc accacagcga
     6001 cagcccgatc agcaccgcac cgatcagccc ggtgaccgtt tcggggatgt gcatcacggc
     6061 cgagaacagc atgatcccgc ccagcgcgat gatcgcccag aacgccccgt gctccagata
     6121 gcggtattcg gccagggtgc cctgcttgac caggtggatc gtcatggacc ggacgaacat
     6181 cgccccgatc gacaggccca gggcaatgac caccatgttg ttggacaggg caaaggcgcc
     6241 gatcacgccg tcaaagctga acgaggcgtc gaggatgttg aggtaaagga acccgccaag
     6301 gcccgagcgg accaccgcgc cctgcagctt catcgcttcc tcgcgcattt cgaggatcgt
     6361 ggagatcgcc tcgaccgcga tgaaggtgac caggcccagc gtgcccgaga ccatgaaggt
     6421 cagcgcgtcg gccgggggca gcagcagcga aatgccgtag agcaccagca gcagcagcgc
     6481 gatttcggcg gccttgatgt tggagacgac gctgagcttg tgctcgatcc attcgatcca
     6541 gtggacgtcc ttctcaccat cgaagaagaa cgacaggccg accatggcga ggaacgcccc
     6601 gccgaaaccg gcgatgccga catgggcgct ggaaacgatc gcttcgtatt cttccgggct
     6661 gttgagcgag aggttgatcg cgtccatcgg ccccaggcca gccgcgactg ccacgatcgc
     6721 cagcggaaag acgattcgca tcccgaacac cgcgatcacc atgccccagg tcaggaagcg
     6781 gcgctgccag acctcgtcca tgtccttcag caccgcggcg ttgaccaccg cgttgtcgaa
     6841 cgacagcgag acttcgagaa tcgagagcac gaagatgatc cacagcatcg aggccgtggc
     6901 accgagatcg tgggtctgcg accagccata ccagacgccc agcgccaggc agacgacggt
     6961 gaacaggatc gagcccttgt agaacttcat cagcatggga gccgccctat ttcggctggt
     7021 aggtctgttc aatccccggg aaggatctat cccgaacttc cgccgcatag cgggcagcgg
     7081 cggcggagat cgtctcggcg atctcttcgt aacgcttcac gaaccgggga acgcgctcga
     7141 acatgccgag catgtcctcg gtcaccagca cctgcccatc gcactgcgcc gaggcgccga
     7201 tgccgatcgt cgggcaggcc accgccttgg tcgcggcgat cgccacgggt tcgaccacgc
     7261 cttcgatcac gatcgcgaag gccccggcct ggtccagcgc cttggcgtcg gacacgatct
     7321 tctcggcctc ggcctcgctg cgcccgcggg cgccatagcc gccgagcagg ttgaccgcct
     7381 gcggggtcag gccgacatgg cccatcaccg gaatgccccg gcgggtcagg aagtcgaccg
     7441 tctcggccat cgccgcgccg ccttccagct tgatcgcgtc gcagccggtt tccttgagaa
     7501 ggcgcgccgc gctggtgaag gcctgttcct tggatccctc gtaggttccg aacggcaggt
     7561 cgacgaccac cgccgcgtga taggacccgc gcaccacggc ggcaccgtgc gccgccatca
     7621 tttccagcgt caccggcacc gacgagggca ggccgtagat cacctggccc agcgaatccc
     7681 cgcacagcag cagatcgcaa tgcacatcaa gcagttgcgc ctggcgggcg gtataggcgg
     7741 tcagcatgac caccggctcg gccgtgacgc cgtcctgctt gcgctgacgg atcgtgggga
     7801 tcgtcagccg ccgcagcggg gcgggggtgg gatgggcgcg gctggtggcc gtatcgagct
     7861 ggtaggtggt cgacatgggg gccctttaga cccgttccac aatctgggga aggcttggca
     7921 gttagcgctt gccaatacgg ggcgggccgc tagcttccgc gcccagggaa agacacgcgg
     7981 tttcggcacg tatcgaaccg ccaaattagg ggaaatccat gttcggacgc gtcaaaccac
     8041 tagacgccat cctggccact gccgaaaaga agtcgctcca ccgctcgctt ggcgctttcc
     8101 agctgaccat gctcgggatc ggggcgatca tcggcaccgg gatctttgtt ctgaccgccg
     8161 aagcggcgca gaaggccggc ccggggatga tgctcagctt catcatggcc ggcttcgtct
     8221 gcgccgttgc ggcgctctgc tacgccgaaa tggcgtcgat ggtgccggtt tccggttcgg
     8281 cctacactta ttcctatgcc tcgatgggcg aaatcgtcgc ctggatcgtg ggctgggcgc
     8341 tgatcctcga atacgcgatc gcggcctccg cggtgtcggt cgggtggtcg aactacttcg
     8401 tcgggctggt ccaatccggc ctggggatgg agttgccaac cttcttcacc aagggcgcct
     8461 ttgccgcggt gggcggcggg atcaacatcc cggcgatctt cgtggccctg ctggtgaccg
     8521 ccctgctggt gatcggcacc aaggaatcgg ccacgttcaa cgccgtgctg gtggtgatca
     8581 aggtggttgc gctgaccgcg ttcatcatcc tggcggtgcc ggtgatgaag atggaaaact
     8641 tcgagccgtt ctcgccgctc ggctttggcg gggtctcggc ggccgccgcc tcgatcttct
     8701 tcgcctatgt cggctttgac gcggtttcga ccgcggccga ggaaaccaag aacccgcagc
     8761 gcaacatgcc gatcggcctg atcggcagcc tggtgatctg caccatcttc tatctgctgg
     8821 tggcggcagg cgcgatcggc gctgtcggcg cgcagccggt gatgggtgcg gcgggcgaag
     8881 ccttggctac cggcagcaag gaactgcagg cccagtgcgc cgccctgacc agcgcgggca
     8941 ctgttccgct ggtctgctcg gacgaagcgc tggcccatgt cctgcgtgag atcgggcatg
     9001 tccaggtcgg caacctgatc ggcctggccg cgttcctggc gctgccgtcg gtggttctga
     9061 tgatgctgtt cggccagacc cgcatcttct tcgtgatggc ccgtgacggc ctgctgcccg
     9121 aagggctggc caaggtccac ccgaagttca agaccccgca cgtggttacc atcatcaccg
     9181 gtatcttcgt gaccatggcg gccgcgttct tcccggttgg ccagctggcg gacatctcga
     9241 actcgggcac gctgttcgcc ttcatgaccg tggcgatcgg cgtgatgatc ctgcgccgca
     9301 ccgcgccgga ccggcaccgt ccgttccgca ccccgttcgt ctggctggtc ggcccggcgg
     9361 cggtggcggg ctgcgcctac ctgttcttca acctgtcggg ctataccgag ctgatgttcc
     9421 tcggctgggc cgcgattggc ctggtggtct acttcgccta tggccgccgc aagagccacg
     9481 tcggccgcgg gctggtggaa gtgcacgagc aggatgccga tgctccgccg cagccggttc
     9541 cgccgctgcc cggcgggatc gacatcaacg actgacctgg tttccaggtg gggagagggg
     9601 ccgttcccgg tggggagcgg cccttttctt gtgtccgccc tgtccctgcg gacagggccc
     9661 tgcaa
//
LOCUS       DASUDV010000311         9676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2086534,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000311 DASUDV010000000
VERSION     DASUDV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086534"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="gcvT"
                     /locus_tag="VFV30_06765"
     CDS             complement(<1..1041)
                     /gene="gcvT"
                     /locus_tag="VFV30_06765"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX4847828.1"
                     /translation="MSDEPETEDESPIETLILPLDAWHRARGGRMVEFAGYWMPVQYE
                     GILAEHQWTREHAGLFDVSHMGQLYLSGEGVEAAIEAVLPIDLATLKPFGPRYSMLLD
                     EDGGILDDLMVTRWSTGFYLVVNGATKWDDMAHLRENLPDEVTMTHLEDSALLALQGP
                     EAFAALERVTTGEHPLSALTFMKGGQFKIDGVDAWISRSGYTGEDGFEIAIPAEEAAR
                     VADLICAQPEVKPIGLGARDSLRLEAGLPLYGHDLSPERSVVEADLLFAVNKRRRSEG
                     GFLGAERVLRELAEGTAQVRVGLSLDGRQAAREGAEVYAGATKVGVLTSGGFSPTLGH
                     PIAMAYVDRAQAA"
     regulatory      complement(1072..1175)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(1176..1283)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1486..2172)
                     /locus_tag="VFV30_06770"
     CDS             complement(1486..2172)
                     /locus_tag="VFV30_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX4847829.1"
                     /translation="MSKPTRIALVGATGLIGMSLIRLAVNRPDIRVIAISRREVALPP
                     GARMEVLLAQPEGWGDAIAAANADVLVSALGTTWKKAGKDEAAFRSVDQELVLACARA
                     AKAAGIRQMISVSSVGADPLSKTFYLRVKGETDQALGKVGIPRLDILRPGLLRGPREE
                     LRPAEHLGRILSPVADLLMQGAYRRYRSIRSETMAEAIVGLTREAMAGRFVFEHDAIL
                     RAARKGQSIL"
     gene            complement(2165..3334)
                     /locus_tag="VFV30_06775"
     CDS             complement(2165..3334)
                     /locus_tag="VFV30_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX4847830.1"
                     /translation="MTVGGPSPFACDPAQSRGREFPIEMALARGPRSAFQRDRDRVVH
                     SIAFRRLRHKTQVFIAPDGDHYRVRLTHSLEVAQIGRVIARALGLDEDLTEALCLAHD
                     IGHPPFGHAGEEALDNALIRHGGFDHNAHCLRVLMRLESPYCEHEGLNLSWEMLEGLA
                     KHNGPLGMVPWALAELDAAFPLELREWGSLEAQVAALADDIAYDNHDIDDGLRAGFLD
                     LEELLTLDFVADQWRAIETKFPGAPQDRRLRELVRSQIGLMVNDLIGETAVRAKGMAS
                     AADVRAAGRATAAFTPAMAESERQLKRFMYEKLYHHPQQLQTAERARSVTAELYAAYA
                     QEPKLMDAGWFERLPDQEPERSRHIADFIAGMTDRYAISAHARIYGRTPEGLRNV"
     gene            complement(3331..4263)
                     /locus_tag="VFV30_06780"
     CDS             complement(3331..4263)
                     /locus_tag="VFV30_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847831.1"
                     /translation="MSAPTESASALELPGRVVWFGNQPVLEWRRLDLADALGPRFYES
                     ALKAPVVRIQALDTIPEEGLERVAGLILHAWRCGSTLLCALFNTAPRTVALSEPSCFS
                     DLLVGIEQEPALAAARIRKLAACFGHALAGVADRVVIKLPGLLAQHAEEIEDALPEVP
                     VIFLHREAGAVVQSLLREPIGNLSAAPARYRGSMPASAAPESLLFTASIIAENTRAIA
                     KTRRVHCCDYRHLPALTWQGIAGHFGLALSEADCQRMAEAAQWHSKFRPGVRRFTGDA
                     AVDPVPGAEQAVAVIASALHYLEEAGPLVIEGVE"
     gene            complement(4281..5012)
                     /locus_tag="VFV30_06785"
     CDS             complement(4281..5012)
                     /locus_tag="VFV30_06785"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX4847832.1"
                     /translation="MEQGLPTRRSIVLRKIGLIGGMSWLSTRTYYEHINKKVQARVGD
                     HASAPMLIESLDFAGLARLSSPDAWKQAAEVLAGSAQRLEAAGATALLIGANSMHKVY
                     DQVAAAVAVPVIHIADCVASKMEADGVKKAALIGTRNVMVESFYRQKLIKRDIELLPP
                     EMGFVETLDRIIYDELLLGKASRQAERELKTILTNLAQDGAEAVVLGCTELEMIVDVD
                     ANVLPIYDCTRLHAQAAVDWILSGG"
     gene            complement(5042..6031)
                     /locus_tag="VFV30_06790"
     CDS             complement(5042..6031)
                     /locus_tag="VFV30_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HEX4847833.1"
                     /translation="MRLLAAALLLAGAAFASRAEPAAPGVNARAVADAGYPASLSAYG
                     FFADPAGQVPAAGVTPYRVNTPLWSDGAEKLRFVYVPPGSRALADGDGLLKFPVGSAL
                     IKTFKLEGRLIETRVLLHRAEGWVALPYQWNAEQTDARLVLAGARLDAKTPAGEAISY
                     AIPNKNQCKECHGLGGEVVPLGPKARNLSTAWLQAFAGSGRLDRAPKVARPVPLWEDR
                     AKVPVADAARGYLDANCAHCHNPQGAASNSGLYLAWEVTDPVMLGIGKRPVAAGRGSG
                     GLDFGIVPGKPEQSILYHRMASLEGGVSMPEVGRASLDRDGLAVVHAWIRGLR"
     gene            complement(6028..7227)
                     /locus_tag="VFV30_06795"
     CDS             complement(6028..7227)
                     /locus_tag="VFV30_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HEX4847834.1"
                     /translation="MSALLIAAATALALAAPVHAKTITVAAGEGAQTRLQEALIVAEP
                     GDVVQLEAGRFHLTDGLSLDVKGVTVRGAGMDATVLDFSGQKGAGEGLLVTSDEVTLR
                     DFAVENTRGDGIKSKGADNIVYYRVRVTWTGGPKTTNGAYGIYPVSSTGVLVDGCKVS
                     GASDAGIYVGQSKGITVRYNFVENNVAGIEIENSRDALVTDNLVTRNTGGILVFDLPN
                     LPVMGGGNTIIKNNTVVGNDTPNFAPKGNIVAGVPRGTGIMVMANEGVWVSQNRMAHN
                     PTAHVLVVAYPQPFTDKTYNPLPRSVLIEDNSFVGGGDDPQMPGGQQLVQMLGGSLPP
                     VVWDGVGPNPPVVPDGTSFLSLGLPGQGVGMAEARPRMIENTKTAAMVPPGRFGAPAA
                     LDARARP"
     gene            complement(7286..7612)
                     /locus_tag="VFV30_06800"
     CDS             complement(7286..7612)
                     /locus_tag="VFV30_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847835.1"
                     /translation="MTDRSALGLALCLTAALAACAPSKLAYGQVKSAMLEAGLSEPNA
                     ACMAERMTDRLSLGQLNKLKQLRGEKRSLTDYVNAVRRVGDAEALSVTASSAALCASG
                     LAGEKR"
     gene            7727..8245
                     /locus_tag="VFV30_06805"
     CDS             7727..8245
                     /locus_tag="VFV30_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847836.1"
                     /translation="MTEPPKPQYTVVEREGRLVVEKAGEPAKGRLGKIPFPGLTPSNF
                     DWLKTGQALLTRYYSPKLTDDGQAVMPASQLEPYIKLSARPDDLYYLTPRQQAIIGYL
                     TALHRNGIIVFVVITAMAVIFESGVFLLAIALAIATATLTTYFKDEYEAIRQQLYVPA
                     TPPAPEGGPPLA"
     gene            complement(8242..>9676)
                     /locus_tag="VFV30_06810"
     CDS             complement(8242..>9676)
                     /locus_tag="VFV30_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX4847837.1"
                     /translation="AWAMLAYLASGVLFILALRGLSSPGTSRAGNRYGMLGMTIAVVT
                     TLVTHVPMITGDATNSYVGPDFLSLGEILAAVAIGGGIGWVIARRIAMTDMPQLVAAF
                     HSLVGLAAVLVGWAAYLNPEAFAIVGADGQINPVSRLELGLGIAIGAITFSGSVIAFL
                     KLAGKMSGAPILLPARHVINLGTLLAILGLTGFFMTDQSLWVIATITVLAFLIGFLLI
                     IPIGGADMPVVVSMLNSYSGWAAAAMGFTLSNTAMIITGALVGSSGAILSYIMCKAMN
                     RGFLSVIAGGFGAEAAATGEAREQRPWKRGSAEDAAYMLAEAEKVIIIPGYGMAVSQA
                     QHALREMGDLLKAKGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFA
                     TADVAFIIGANDVVNPAAKTDKSSPIYGMPVFDVDKAKQVFFIKRSMGGVGYAGVDND
                     VFYMDQTLMLLADAKKMVEDINKGLAH"
BASE COUNT         1609 a         3356 c         3138 g         1573 t
ORIGIN      
        1 cgcggcctgg gcgcggtcga cataggccat ggcaatcggg tggccgagcg tgggcgagaa
       61 cccgccggag gtcagcacgc cgaccttggt ggcgccggca tagacctcgg ccccttcgcg
      121 cgcggcctgg cggccatcga gggaaaggcc gacgcggacc tgggcggtcc cttcggccag
      181 ctcgcgcagc acccgctcgg cgccgaggaa cccgccctcg ctgcggcggc gcttgttgac
      241 cgcgaacagc aggtcggcct caaccaccga acgctcgggc gagagatcgt ggccgtaaag
      301 cggcagcccg gcttccagcc gcagcgaatc ccgcgcgccc aggccgatcg gctttacctc
      361 cggctgggcg cagatcagat cggccacgcg ggcggcctct tcggcgggga tggcgatctc
      421 gaacccgtcc tcgccagtat agccagagcg gctgatccag gcatcgaccc cgtcgatctt
      481 gaactgcccg cccttcatga aggtcagcgc cgaaagcgga tgttccccgg tggtgacgcg
      541 ctccagcgcg gcaaacgcct ctggcccctg cagcgccagc agcgcgctgt cttccaggtg
      601 ggtcatggtc acttcgtcgg gcaggttctc gcgcaggtgg gccatgtcgt cccacttggt
      661 cgcgccgttg accaccaggt agaacccggt cgaccagcgg gtgaccatca ggtcgtccag
      721 aatgccgccg tcttcatcga gcagcatcga atagcgcgga ccgaacggct tcagcgtggc
      781 cagatcgatc ggcaggaccg cttcgatcgc ggcctccacg ccctctccgg agagatagag
      841 ctggcccatg tggctgacat cgaacagccc cgcgtgctca cgcgtccact ggtgttcggc
      901 caggatccct tcgtattgca ccggcatcca gtagccggcg aattcgacca tgcggccgcc
      961 ccgggcgcga tgccaggcgt cgagcggcag gatcagggtt tcaatcgggg attcgtcttc
     1021 ggtttcaggt tcatcagaca cagggcacgg ctccgcacag aacagacagt ctgtgccccc
     1081 tctgtcacgg aaacctgaga gctttcccgg gcacgggccc ggttacccct tcggcggccc
     1141 cggacggatc cggaacactt tccagagcat cgctatcgac ccacgcggtc cgttggcctg
     1201 agagattccg gggcggttgc tccttcggcg cccggtgaac cgtccgttgg gacgaatcac
     1261 cggaactctc ccgcgcgatc agcgacgctt atgggcatga cggcccgggc taatggtgtc
     1321 aagcagcggc ggcgcgttcc tccccggaac ggggaggggg accgccgcga agcgggggtg
     1381 gaggggcacg ggcctcaatt ccggaccttg ccgcgccgta gggaagaact gtgcgtgccc
     1441 ctccaccatc ctgcggatgg tacccctccc ccagcggggg aggaactaca agatgctctg
     1501 ccccttgcgc gcggcgcgca ggatcgcgtc gtgctcgaac acgaagcgcc cggccatcgc
     1561 ttcgcgcgtc agcccgacga tcgcttcggc catcgtttcg ctgcggatcg agcggtagcg
     1621 gcggtaggcg ccctgcatca gcagatcggc caccgggctg aggatccgcc ccagatgctc
     1681 cgccgggcgc agctcttcgc gcgggccgcg cagcaggccg gggcgcagga tgtcgagccg
     1741 ggggatcccg accttgccca gcgcctggtc ggtctcgccc ttgaccctga ggtaaaaggt
     1801 cttggacaac ggatcggccc cgacagagga aacggaaatc atctggcgga tcccggccgc
     1861 cttggccgcg cgggcgcagg ccagcaccag ttcctgatca acgctgcgga acgcggcttc
     1921 gtccttgccg gccttcttcc aggtggtgcc cagggccgat accagcacgt cggcattggc
     1981 ggcggcgatc gcatcgcccc atccctcggg ctgggccagc agcacttcca tccgcgcgcc
     2041 gggcggcaag gccacttcgc gccgggaaat ggcgatgacc cggatatcgg ggcggttcac
     2101 cgccagccgg atcaggctca tcccgatcag tccggtcgcg ccgaccaggg caatgcgggt
     2161 gggcttagac attgcgcaaa ccctccggcg tgcggccata gatgcgggcg tgggcggaaa
     2221 tggcgtagcg gtccgtcatc ccggcgatga aatcggcgat gtggcggctg cgctccggct
     2281 cttgatccgg cagccgttcg aaccagccgg cgtccatcag cttcggctcc tgggcatagg
     2341 cggcatagag ctcggccgtg accgagcggg cccgctcggc ggtctgcagc tgctgcgggt
     2401 ggtgatagag cttctcgtac atgaagcgct tcagctgccg ctcgctctcg gccatggccg
     2461 gggtgaaggc cgcggtggcc cgcccggcag cgcggacatc ggcggcactg gccatgccct
     2521 tggcccggac cgcggtttcg ccgatcagat cgttgaccat cagcccgatc tggctgcgga
     2581 ccagttcgcg cagccgccgg tcctgcggcg cgcccgggaa cttggtctca atcgcgcgcc
     2641 actggtcggc cacgaaatcg agcgttagca gctcttccag atcgaggaac ccggcgcgca
     2701 ggccatcgtc gatatcgtgg ttgtcatagg cgatgtcatc cgccagcgcg gcgacctgcg
     2761 cttccagcga accccattcg cgcagctcca gcggaaaggc cgcgtccagt tcggccagcg
     2821 cccaaggcac catgcccagc ggcccgttgt gcttggccag cccttccagc atttcccacg
     2881 acaggttcag gccttcgtgt tcgcaatagg ggctttccag ccgcatcagc acgcgcaggc
     2941 agtgggcgtt gtgatcgaat ccgccgtgcc ggatcagcgc gttgtccagc gcctcttccc
     3001 cggcgtggcc gaaaggcggg tggccaatat cgtgggccag gcacagcgcc tcggtcaggt
     3061 cctcgtccag ccccagggcg cgggcgatga cccggccgat ctgggccact tcgaggctgt
     3121 gggtcagccg gacgcggtaa tgatcgccat cgggcgcaat gaacacctgg gtcttgtggc
     3181 gcagccggcg aaacgcgatc gaatggacca cccggtcgcg gtcgcgctga aaggcgctgc
     3241 gcggcccgcg ggccagcgcc atctcgatcg ggaactctcg cccccggctt tgggccggat
     3301 cgcaggcgaa tggtgatggc cccccgacag tcattccacg ccctcgatca caagcggtcc
     3361 tgcttcttcc agatagtgaa gcgcggaagc gataacggca acagcctgtt ccgcaccggg
     3421 aacgggatca accgcggcat cgccggtaaa gcggcggacg cccggacgaa acttcgaatg
     3481 ccattgcgcg gcctctgcca tccgctgaca gtcggcttcc gacagcgcca gtccgaaatg
     3541 ccccgcaatg ccctgccacg tcagagcggg caaatgccgg tagtcgcagc agtggacacg
     3601 ccgggtcttc gcgatcgccc gggtattctc ggcgatgata ctggccgtga agagaaggct
     3661 ctccggagcc gcagaagcag gcatggaacc gcggtagcgt gctggtgcgg cggacaggtt
     3721 gccgattggc tcgcgcagca gcgactgcac caccgcgccg gcttcccggt gcagaaagat
     3781 cacgggaacc tcgggcaaag cgtcctcgat ctcctccgcg tgctgcgcca gcaggcccgg
     3841 aagcttgatc accacccgat ccgcaacacc ggccagcgca tgaccgaagc aggccgcaag
     3901 tttccgaatc cgggccgctg cgagggccgg ttcctgttcg atgccaacca ggagatcgga
     3961 aaagcaagag ggttcactca gcgcaacagt gcgcggggcg gtattgaaca gggcgcagag
     4021 cagggttgac ccgcagcgcc atgcgtgaag aatcaaaccc gcaacgcgtt cgaggccctc
     4081 ctctggaatg gtgtcgagag cctggatccg cacgaccggc gccttcaggg cactttcgta
     4141 aaagcgggga ccaagtgcat cggccagatc gagccgacgc cactccagga ccggctggtt
     4201 gccgaaccag acgacccgcc ctggcagttc gagcgcgctt gcggattcgg ttggcgcgct
     4261 catggcatcc gcatccgccc tcacccgccg ctcaggatcc agtcgaccgc cgcctgggcg
     4321 tggaggcggg tgcagtcgta gatcgggagg acattggcat ccacgtcgac gatcatttcc
     4381 agttcggtgc agcccagcac cacggcctcg gcgccgtcct gggccaggtt ggtcaggatc
     4441 gtcttcagct cgcgctcggc ctggcggctc gccttgccga gcagcagctc gtcatagatg
     4501 atccggtcca gcgtttcgac aaagcccatt tccgggggaa gcagttcgat atcgcgcttg
     4561 atcagcttct ggcggtagaa gctttccacc atcacgttgc gggtgccgat cagcgcggcc
     4621 ttcttgaccc cgtcggcttc catcttgctg gcgacacagt cggcaatgtg gatcaccggc
     4681 accgcaaccg cggcggccac ctgatcatag accttgtgca tcgaattggc accgatcagc
     4741 agcgcggtgg ccccggccgc ttcgagccgc tgggccgatc cggccagcac ctcggccgcc
     4801 tgcttccacg cgtcgggcga ggacagccgg gcaaggcctg cgaaatcgag gctctcgatc
     4861 agcatcggcg cgctggcgtg atcgccgacg cgggcctgca ccttcttgtt gatgtgctcg
     4921 taataggtac gggtcgacag ccagctcatg ccgccgatca ggccgatctt gcgcaaaacg
     4981 atactcctgc gggttggaag cccctgttcc atcgcgcctt aggcgaggcg cgggccaagg
     5041 tctagcgcaa gccccttatc caggcgtgaa cgacggccag cccgtcgcgg tccaggctcg
     5101 cccggccgac ctcgggcatg gaaaccccgc cttccaggct ggccatgcgg tgatagagga
     5161 tcgactgttc gggcttgccc ggcacgatcc cgaagtccag cccgcccgat ccgcgccccg
     5221 ccgcgaccgg gcgcttgccg atccccagca tgaccggatc ggtcacctcc caggccaggt
     5281 agagcccgga attggacgcc gcgccctgcg ggttgtggca atgcgcgcaa ttggcatcga
     5341 gatagccgcg cgcggcatcg gccaccggca ccttggcccg gtcttcccac agcggcaccg
     5401 gccgggcgac cttgggcgcg cggtccagcc ggccggagcc ggcaaaggcc tgcagccagg
     5461 cggtggagag gttgcgcgcc ttcgggccca gcggcaccac ctctccccca agcccgtggc
     5521 attccttgca ctggttcttg ttgggaatgg cataggagat tgcctccccc gccggggtct
     5581 tcgcatcgag ccgcgcgccg gccagaacca gccgcgcatc ggtctgctcc gcgttccact
     5641 gatagggcag cgccacccag ccttcggcgc ggtgcagcag cacccgggtc tcgatcaggc
     5701 gcccttccag cttgaaggtc ttgatcaggg ccgatccgac cggaaacttc agcaggccat
     5761 cgccatcggc cagcgcgcgg ctgcccggcg gcacatagac gaaccgcagc ttctctgccc
     5821 cgtctgacca cagcggcgtg ttgacccggt agggagtgac cccggccgct ggcacctgcc
     5881 cggccggatc ggcgaaaaag ccgtaggcgg agaggctggc cggatagccc gcatccgcca
     5941 ccgcgcgcgc attgaccccg ggcgctgccg gctcggcgcg cgaggcaaag gcggcgcccg
     6001 ccagcagcag cgcggcggcc agcagcctca tggccgggcg cgggcgtcca gcgcggcggg
     6061 ggctccgaac cgacctggcg gaaccatggc cgcggtcttg gtattttcga tcatccgcgg
     6121 ccgcgcttcg gccatgccga ccccttgccc cggcagcccg agcgagagga acgaggtgcc
     6181 gtccggcacg accggcggat tggggccaac cccatcccac acgaccgggg gcaggcttcc
     6241 gcccagcatt tggacgagct gctgcccgcc tggcatctgc ggatcgtctc cgccgccgac
     6301 aaagctgtta tcctcgatca gcaccgaccg cggcaggggg ttgtaggtct tgtcggtaaa
     6361 cggctgcgga taggcgacca ccagcacgtg ggctgtggga ttgtgcgcca tccggttctg
     6421 gctgacccag acaccttcgt tggccatcac catgatcccc gtgccgcgcg gcactccggc
     6481 gacgatgttg cccttgggcg caaagttggg cgtgtcgttg cccaccacgg tgttgttctt
     6541 gatgatcgtg ttgcccccgc ccatcaccgg caggttgggc agatcgaaca ccaggatccc
     6601 gccggtgttg cgggtgacca gattgtcagt gaccagcgca tcgcggctgt tctcgatctc
     6661 gatcccggcc acgttattct caacgaaatt gtagcggacc gtgatgccct tggactggcc
     6721 gacatagatc ccggcgtcgc tcgcccccga taccttgcag ccgtcaacca gcaccccggt
     6781 gctcgacacc ggatagatcc cgtaagcgcc gttggtggtc ttgggaccgc cggtccaggt
     6841 cacgcggacg cggtaataga cgatattatc cgcgcccttg ctcttgatcc cgtcgccacg
     6901 cgtgttctcc accgcgaaat cgcgcagggt gacctcgtcg gaggtgacca gcagcccctc
     6961 gcccgccccc ttctggccgg aaaagtccag cacggtcgcg tccatcccgg ccccgcggac
     7021 cgtcacgcct ttcacgtcca gactaagccc gtcggtcagg tggaaccgcc cggcctcaag
     7081 ctgcaccacg tcgcccggct cggccacgat cagcgcctcc tgcagccggg tctgcgcgcc
     7141 ctctccggcg gcgaccgtga tggtcttggc gtgaacgggg gccgccaggg caagcgccgt
     7201 ggccgcagcg ataagcagtg cggacatcct catggggcgc gatgatggac gggccgccgg
     7261 gtaagtcaat cccgggtcct gcggcctagc gcttctctcc ggccagtccg ctggcgcaca
     7321 gcgccgcgct gctggcggtg accgagagcg cctcggcatc gccgacccgc cgcacggcgt
     7381 tgacatagtc ggtcaggctg cgcttctcgc cgcgcagctg cttcagcttg ttgagctgcc
     7441 ccaggctgag ccggtcggtc atccgctcgg ccatgcaggc ggcgttcggc tcgctcaggc
     7501 ccgcctccag catggcgctc ttgacctggc cataggccag cttgctgggc gcacaggcgg
     7561 ccagtgcagc cgtgagacac agagcaaggc caagtgccga tcgatccgtc atgacgtttc
     7621 ccccagggtg atcccgataa cgaggccggg ccgataatct gcggcaactt tcgcacggct
     7681 gaaccggccc ttgtcaaccc gcgccgaccg ggcctaccct cgccggatga ccgagcctcc
     7741 caagccccag tacaccgtgg tcgaacgcga aggccgcctg gtggtcgaaa aggccggtga
     7801 gcccgccaag ggccgccttg gcaagatccc gttcccgggg ctgacgccgt cgaatttcga
     7861 ctggctgaaa accggccagg ccctgctgac ccgctactac agtccgaagc tgaccgacga
     7921 cggccaggcc gtgatgcccg ccagccagct ggaaccttat atcaagctct ctgcccggcc
     7981 tgacgatctc tattacctga ccccccgtca gcaggcgatc atcggctacc tcaccgcgct
     8041 gcaccggaac ggcataattg ttttcgtggt gatcacggca atggcggtga tctttgaatc
     8101 gggcgtgttc ctgctggcca ttgcccttgc catcgcaacg gcaacgctga ccacgtactt
     8161 caaggacgag tacgaggcga tccgccagca gctttacgtg ccggccacgc cccctgcgcc
     8221 agagggcggg ccgcccttgg cctagtgcgc caagcccttg ttgatgtcct cgaccatctt
     8281 cttcgcatcg gccagcagca tcagggtctg gtccatgtag aacacgtcgt tgtcgacccc
     8341 ggcatagccc acgccgccca tgctacgctt gatgaagaac acctgcttgg ccttgtcgac
     8401 gtcgaacacc ggcatgccat agatcggcga tgacttgtcg gtcttggctg ccgggttgac
     8461 cacgtcgttc gcgccgatga tgaaggcaac gtcggcggtg gcgaactcgc tgttgatgtc
     8521 ttccagctcg aacacctcgt cataaggtac gttggcttcg gccagcagca cgttcatgtg
     8581 cccgggcatg cggcccgcca cggggtggat cgcgtacttc acttccacgc ccttggcctt
     8641 cagcagatcg cccatttcgc gcagcgcgtg ctgggcttgg gacaccgcca tgccatagcc
     8701 ggggatgatg atgaccttct ccgcctcggc cagcatatag gccgcgtcct cggcgctgcc
     8761 gcgcttccac gggcgctgtt cgcgcgcttc gccggtggcc gccgcttcgg cgccaaagcc
     8821 gcccgcgatc acgctcagga acccgcggtt catcgccttg cacatgatgt aagacaggat
     8881 cgcgccggac gagccgacca gcgccccggt gatgatcatc gcggtgttgg aaagggtaaa
     8941 acccatcgcc gccgcagccc agcccgagta cgagttgagc atcgagacca cgaccggcat
     9001 gtccgccccg ccgatcggaa tgatcagcag gaagccgatc aggaaggcca gaaccgtgat
     9061 cgtggcgatc acccacaggc tctggtcggt catgaaaaag ccggtcaggc cgaggatcgc
     9121 cagcagcgtg cccaggttga tcacgtgccg cgccggcagc aggatcggcg cgccgctcat
     9181 cttgccggcg agcttgagga aagcgatgac cgagccagag aaggtgatgg ccccgatcgc
     9241 gataccaagg cccagttcca gccgcgaaac cgggttgatc tgcccgtccg ccccgacaat
     9301 cgcgaaggct tcgggattga ggtaggccgc ccagccgacc agcactgcgg ccaggccgac
     9361 caagctgtgg aacgccgcga ccagctgcgg catgtcggtc atcgcgatgc gccgcgcgat
     9421 gacccagccg atgccgccgc cgatggcgac cgcggccagg atctcaccca gcgagaggaa
     9481 atcggggccg acatagctgt tggtggcatc gccggtgatc atcggcacgt gcgtaaccag
     9541 cgtggtcacc accgcgatcg tcatgcccag catgccatag cggttgcccg cgcggctggt
     9601 gccggggctg gacagcccgc gcagcgccag gatgaacagc acgcccgaag cgagataggc
     9661 cagcatggcc caggcc
//
LOCUS       DASUDV010000312         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_54436,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000312 DASUDV010000000
VERSION     DASUDV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54436"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VFV30_06815"
     CDS             complement(<1..1325)
                     /locus_tag="VFV30_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4847838.1"
                     /translation="MASLAPEAPKFTHWNQSQPKDALAHIPGEDGWPILGNTLQLLAD
                     PTGFGNRMRAKYGDIYRNNSFGRRGVIMFGADANELVLFDRDKIFSSEQGWGPVLDQL
                     FPRGLMLLDFDHHRADRRALSIAFKPGPMRHYSGALNAGISARAEEWRGQSILFYPAI
                     KQLTLDLAADSFLGVPLGPEADAINKAFVDMVAASIGVVRKPLPFTAMGRGVAGRKFL
                     LDYFTRETLKRREAGGGQDMFSQFATATREDGSLLPVDEVVDHMIFLMMAAHDTITSS
                     ATSLVWLLAKNPEWQDKLRAEILAVTGGEGRPLSYDDLGKLELTEMAFKEALRLIPPV
                     PSLPRRALKDFTFKGVRVPAGTPVGISPAAVHMEEEHWPEPAKFDPMRFTPEQVAGRH
                     KYAWVPFGGGAHMCLGLHFAYMQVKILLAHLLSRYQIEIEPGYAPEWQP"
     gene            complement(1376..>2120)
                     /gene="hemH"
                     /locus_tag="VFV30_06820"
     CDS             complement(1376..>2120)
                     /gene="hemH"
                     /locus_tag="VFV30_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX4847839.1"
                     /translation="GQAEALQARLGDGVMVRWAMRYGSPAIGAVLAEMKAAGCERILV
                     APLYPQYSAATTATAIDELGRQLTAMRWQPSTRTLPPYYDDPLHVAALRDDLARQLRA
                     LPFVPEVLLLSFHGMSERTLHLGDPYHCQCRKTARLLGEALTADFPGLRVETSFQSRF
                     GRAKWLEPYTEDTLTALATGGIKRLAIAAPGFSADCLETREELAIRGREVFEEAGGLE
                     FAALDCLNTSDAGMAMLEALVRRELGGWI"
BASE COUNT          379 a          710 c          684 g          347 t
ORIGIN      
        1 cagggctgcc attcaggcgc gtaaccgggc tcgatctcga tctggtagcg gctcagcagg
       61 tgcgccagca ggatcttcac ctgcatatag gcaaagtgca gccccaggca catgtgcgcc
      121 ccgccgccga acgggaccca ggcgtacttg tgccgccccg cgacctgttc aggcgtgaag
      181 cgcatgggat cgaacttggc cggttcgggc cagtgctctt cctccatgtg gaccgccgcc
      241 gggctgatgc ccacgggggt gccggccggc acgcgcacgc ccttgaaggt aaagtccttc
      301 agcgcgcggc gcggcagcga ggggaccggc ggaatcagcc gcagcgcttc cttgaaggcc
      361 atctcggtca gttccagctt gccgagatcg tcataagaca gcgggcgtcc ttcaccgccg
      421 gtgacagcca ggatctcggc gcgcagcttg tcctgccatt cggggttctt ggccagcagc
      481 cagaccagcg aagtggccga gctggtgatc gtgtcatgcg ccgccatcat caggaagatc
      541 atgtgatcga ccacttcgtc gaccggcagc agcgaaccat cctcacgcgt cgctgtcgcg
      601 aactggctga acatgtcctg cccgccgcct gcttcgcgcc gcttcagcgt ttcacgggtg
      661 aagtaatcga gcaggaactt gcggccagca acgccgcggc ccatcgcggt aaagggtagc
      721 ggcttgcgca ccacgccgat cgaggcagct accatgtcga caaaggcctt gttgatcgca
      781 tcggcttcgg gcccgagcgg cacgcccagg aagctgtccg ccgccagatc gagcgtcagt
      841 tgcttgatcg ccggatagaa caggatcgac tgcccgcgcc attcctcggc ccgggcggaa
      901 atgccggcgt tgagcgcccc ggaatagtgc cgcatcggcc ccggcttgaa ggcaatcgac
      961 agcgcccggc gatcggcgcg gtggtgatcg aaatcgagca gcatcagccc gcgcgggaac
     1021 agctggtcca gcaccgggcc ccagccctgt tcggaggaga agatcttgtc gcgatcgaac
     1081 agcaccagct cgttggcatc ggcgccgaac atgatcaccc cgcgccggcc aaagctgttg
     1141 ttgcgataga tatcgccata cttggcgcgc atccggttgc cgaaacccgt ggggtcagcc
     1201 agcaattgca aggtgttgcc caggatcggc cagccatcct cgccggggat gtgggcgagc
     1261 gcgtccttgg gctgcgactg gttccagtgg gtaaacttcg gggcttcggg ggcaagggag
     1321 gccattagtc atccttttgg acacttactg acaacgatgt aacttaatcg aacgattaaa
     1381 tccatccccc aagctcgcgc cgcaccagcg cctcgagcat ggccatcccc gcatctgacg
     1441 tattaaggca atcgagcgcc gcaaattcaa gccccccggc ctcttcgaac acctcgcggc
     1501 cgcggattgc cagctcctcg cgcgtttcca gacagtcggc cgaaaagccc ggcgcagcga
     1561 tggcgaggcg cttgatcccg ccggtggcga gcgcggtcag cgtgtcttcg gtatagggct
     1621 cgagccactt ggcgcggcca aagcgcgact ggaagctggt ttccacccgc aggccaggga
     1681 aatccgccgt cagcgcctcg cccagcagcc gcgcggtctt gcggcactgg cagtgatagg
     1741 gatcgcccag gtgcagcgtc cgttcggaca tgccgtggaa cgacagcagc agcacctcgg
     1801 gcacgaaggg caaggcgcgc agctgccgcg ccagatcatc gcgcagcgcg gccacgtgca
     1861 gcggatcgtc gtaatagggc ggcagggtgc gggtgctggg ctgccagcgc atcgctgtca
     1921 gctgccggcc cagctcgtcg atcgccgtgg cggtcgtggc ggcggagtat tggggataga
     1981 gcggcgcaac caggatccgc tcgcagcccg ccgccttcat ttcggccagc accgcgccga
     2041 tcgccggact gccatagcgc atcgcccagc ggaccatcac gccatcgccc agccgcgcct
     2101 gcaaagcttc ggcctgcccc
//
LOCUS       DASUDV010000313         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1247359,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000313 DASUDV010000000
VERSION     DASUDV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247359"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFV30_06825"
     CDS             <1..431
                     /locus_tag="VFV30_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4847840.1"
                     /translation="SFGFGADDYVTKPFHREELVARIHAVVRRSKGHSQSVIRTGKLA
                     VNLDAKTVEVDGARVHLTGKEYAMLELLSLRKGTTLTKEMFLNHLYGGMDEPELKIID
                     VFICKLRKKLAHACGGENYIETVWGRGYVLRDPGEEAQAA"
     gene            complement(514..1317)
                     /locus_tag="VFV30_06830"
     CDS             complement(514..1317)
                     /locus_tag="VFV30_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4847841.1"
                     /translation="MQRPPSVLDALTDKEVEILRLLTAGHTVKTIAARLGRSEASINE
                     RLRDARRKTGVGSSRELARLLDAQKIWDKNIDLSPQGSLADRLAQPPVAGRHRSKGTL
                     VMLIASIALAFAAAGPAQQAAAPEAGQAAMAKLPPLVGRWSLDVMRIPEEERPQRVTI
                     EFALAPDKKWTTQVEIVGADGSTMRGQSSAAPDGVPVASTGNMPFIDTVSLRHPSPDT
                     LVMTLGKNGAPVSTRVYTVGKDRKTMTETIIWASTGIPKLETTYFNRID"
     gene            1469..2188
                     /locus_tag="VFV30_06835"
     CDS             1469..2188
                     /locus_tag="VFV30_06835"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEX4847842.1"
                     /translation="MTAHKPGDPTTLNRLYGRSKGKPLRAFQQELVDTLLPQIAVPAE
                     GEVSAERLFGQTCPLHFEIGFGGGEHMAARADMLPDHGFIGAEPFINGVAQALVHVSG
                     DHGAHPPLGNVRIFHGDALDVLRRIPDGTLSFLYLLHPDPWPKARHAKRRMMNDGPVD
                     LFAAKLRPGGEFRFGTDHAVYVRHALMVMRRHRHQFEWLIDKPEDFQKRPGGWPETRY
                     EHKARTVYGHEVWYFRYRKRA"
     gene            2197..2895
                     /locus_tag="VFV30_06840"
     CDS             2197..2895
                     /locus_tag="VFV30_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX4847843.1"
                     /translation="MRTLGLIGGMSWESSAHYYRLINQGVRDARGPTASARCLMWSFD
                     FSEIEQLQRAGDWARLEELMIDAGQRLVRGGAEALVICTNTMHRMADAVEAGAGVPLL
                     HIADPTGAAVRAAGCAKVGLLGTAFTMEQDFYKARLARLHGLDVLVPESADRELVHRV
                     IYDELVAGVVNEHSRAAYRAVIARLVDRGAEAVILGCTEIMLLVGADDSAVPLFDTTA
                     LHARAAVDLALSID"
BASE COUNT          504 a          985 c         1017 g          552 t
ORIGIN      
        1 gcagcttcgg cttcggcgct gacgattacg tcaccaagcc gttccaccgc gaggaactgg
       61 tcgcccgcat tcacgccgtg gtgcgtcgtt ccaagggcca ttcgcagtcg gtgatccgca
      121 ccggcaagct ggcagtgaac ctcgatgcca agaccgtgga agtcgatggc gcccgcgttc
      181 acctgaccgg caaggaatat gcgatgctcg agctgctttc gctgcgcaag ggcaccacgc
      241 tgaccaagga aatgttcctc aaccacctct atggcgggat ggacgaaccc gagctcaaga
      301 tcatcgacgt gttcatctgc aagctgcgca agaagctggc gcatgcctgc ggtggggaaa
      361 actacattga aaccgtctgg ggccgcggct acgttctgcg cgatccgggt gaagaggcgc
      421 aggccgcctg atcttccaga ttaccttgaa gtaaccttgt accggcccgc ttccctggaa
      481 gcgggccgat tttcttgggg ccaccgcggc ggctcaatcg atccggttga agtaggttgt
      541 ttccagtttg gggatgccgg tgctggccca gatgatcgtt tcggtcatgg tcttgcgatc
      601 cttgcccacg gtatagaccc gggtcgaaac cggcgcgccg ttcttgccca gggtcatcac
      661 cagggtgtcc ggcgaagggt gccgcagcga taccgtatcg atgaagggca tattgccggt
      721 gctggcaacg ggcacaccat cgggcgcggc gctcgattgt ccgcgcatgg tggaaccgtc
      781 ggcgccgacg atctcgacct gggtggtcca cttcttgtcg ggcgcgaggg cgaattcgat
      841 ggtcacccgc tgcgggcgtt cctcctcggg gatgcgcatg acatcgagcg accagcgccc
      901 gaccagcggc ggcagcttgg ccatggcggc ctggcccgct tcgggtgcgg cggcctgctg
      961 ggccgggccc gccgcggcga aggcaagcgc gattgaagca ataagcatga caagggttcc
     1021 tttcgacctg tggcgccccg caaccggggg ctgcgccaat cgatcggcca aggaaccctg
     1081 cggcgataga tcgatattct tgtcccagat tttctgggcg tccagcaggc gcgccagttc
     1141 gcggctgctg ccaacaccgg tcttgcggcg tgcatcgcgc aggcgttcgt tgatcgatgc
     1201 ttccgaacgg cccaggcgcg cggcgatcgt cttgaccgta tgcccggccg tcagcagacg
     1261 caggatttcg acttccttgt ctgtaagcgc gtcaagcacg ctgggggggc gttgcatcac
     1321 cgacatgcca cgtgatcacc acaagctctg ccatgcgtcc atcacaaatt ttttgttcag
     1381 acccccgttc tggccgggcc tggcaggctc tcaagctgac cttctgcggc cttgaccccc
     1441 gtcgggcccg ctgccagacg ctcgggccat gactgcgcac aaacctggcg atccgaccac
     1501 gctcaaccgg ctctacggcc gctcgaaggg caagccgctg cgcgccttcc agcaggaact
     1561 ggtcgatacc ctgctgccgc aaatcgcggt tcccgccgag ggcgaggtga gcgccgagcg
     1621 gctgttcggc cagacttgcc cgcttcattt cgagatcggc tttggcggcg gcgaacacat
     1681 ggcggcgcgg gccgacatgc tgcccgatca cggctttatc ggcgccgaac cgttcatcaa
     1741 cggggtagcc caggcgctgg tccatgtatc gggcgatcac ggtgcgcatc cgccgcttgg
     1801 caatgtccgg atcttccacg gcgatgcgct cgatgtgctg cgccgcattc ccgatggcac
     1861 gctgtcgttt ctctacctgc tgcaccccga tccctggccc aaggcgcgcc atgccaagcg
     1921 gcggatgatg aacgacgggc cggttgacct gttcgcggcc aagctcaggc ccggcggcga
     1981 atttcgcttc ggcaccgatc atgcggtcta cgtccgccat gcgctgatgg tgatgcgccg
     2041 ccaccgccac cagttcgaat ggctgatcga caagccggag gacttccaga agcgtcccgg
     2101 cggctggccc gaaacccgct acgaacacaa ggcccgcacc gtctacgggc acgaagtctg
     2161 gtatttccgc tatcggaagc gggcgtgacg gcgcgtttgc ggaccctggg cctgatcggc
     2221 gggatgagct gggaaagctc ggcccattac tatcggctga tcaaccaggg ggtgcgcgat
     2281 gcgcgcgggc ccaccgcttc ggcgcgctgc ctgatgtggt cgttcgattt ttccgagatc
     2341 gagcagctgc agcgcgcggg cgactgggcc cggctcgaag agctgatgat cgatgccggc
     2401 cagcggctgg tgcggggcgg ggcagaagcg ctggtgatct gcaccaacac gatgcaccgc
     2461 atggccgatg cggtagaggc cggcgccggg gtgccgctgc tgcatatcgc cgatccgacc
     2521 ggcgccgcgg ttcgcgccgc cggctgtgcg aaggttggcc tgcttggcac cgccttcacg
     2581 atggaacagg atttctacaa ggcgcgcctt gcgcggctgc acgggctgga cgtgctggtt
     2641 cccgagtccg cggaccgcga actggtgcac cgggtgatct acgatgaact ggtggccgga
     2701 gtggtgaacg agcactcgcg cgccgcctac cgcgcggtga tcgcccggct ggtggatcgc
     2761 ggcgccgagg cggtgatcct gggttgcacc gagatcatgc tgctggtcgg cgcggatgac
     2821 agcgcggtgc cgctgttcga taccaccgcg ctccacgcgc gggccgcagt ggacctcgcg
     2881 ctgtccatcg actgagcgcg atcaggcggg aaccgccaca gcggcgggcg gtcccgcagc
     2941 cagcatggcc tgccattgcc gcgccgagat ggtctggcga aagtgcgcgg cctgggccgg
     3001 atcgaacaat tcgaccagga aggcattctc gacccacagt tcgaccagtt cgaacaag
//
LOCUS       DASUDV010000314         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1026196,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000314 DASUDV010000000
VERSION     DASUDV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026196"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="thrS"
                     /locus_tag="VFV30_06845"
     CDS             <1..963
                     /gene="thrS"
                     /locus_tag="VFV30_06845"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX4847844.1"
                     /translation="DADWMAIKPMNCPAHVLIFKQGIKSYRDLPLRLVENGCCHRNEP
                     HGALHGIMRVRQFTQDDGHIFCREDQIVSEVRSFCQLADRVYKDFGFTYAVKLALRPA
                     KRFGSEADWDKAEQELRQAVIDAGLATEEYGWEELPGEGAFYAPKLEWHLTDAIGRTW
                     QVGTMQSDRVLPQRLDASYVGEDGERHRPVMLHRAIFGSYERFIGILIEHYAGKLPVW
                     LAPVQAVVATIVSDADGYAAEVTRQLAEAGIRVETDVRNEKINYKVRDHSLKKVPHLL
                     VVGKREAEEGTVALRTLGAEHQKVMPLAEAIALLRAEATPPDLR"
     gene            965..1711
                     /locus_tag="VFV30_06850"
     CDS             965..1711
                     /locus_tag="VFV30_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4847845.1"
                     /translation="MIAGLSPEQIAIALAAALAAAFVRGLAGFGMAILLVPVLGLAIP
                     PAEAVVTGNWLGIFIGLVGLRQILSASERSAFIICGFAMAATPLGVWLLAQTDPAMAR
                     LLIALVALGAFVMVLLPKRDEGHQPSGIETGATGIACGVLTGFAGMPGVPVVPFYLRR
                     HLAPVVARASMMTIFLATSVAGVGSAVVLRVATVRELVLGLVLFPAVLLGNWLGHRAF
                     GRISDTAWRSFVGLVLGLSALAALWRLLHA"
     gene            complement(1700..1831)
                     /locus_tag="VFV30_06855"
     CDS             complement(1700..1831)
                     /locus_tag="VFV30_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847846.1"
                     /translation="MARAIAFAAQTSLPPIAVLRPLVVYGCALALILAGPALPALGV"
     gene            1947..3374
                     /locus_tag="VFV30_06860"
     CDS             1947..3374
                     /locus_tag="VFV30_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX4847847.1"
                     /translation="MGQEIATTGFCEGDFARFSARLGAETALLRDLWAGHGLSRQGPI
                     AGFELEAWLIDRNCFPAPHNQAFLARLADPQVVPELSRFNIELNGDPQPLAGDGLARL
                     EQALGHSWQRCVANAHADVDTAIAIGTLPTLRESDLSLAMMTPSNRYAALNAELARQR
                     GGKPVRIDIASAFAGGEHLVSVHDDVMLEAATTSFQLHLQVPADEIAATLNASLALSA
                     PLLAISANSPFLFGKALWHETRIALFEAAFEQAPINGSLRRVSFGSGWCGTDPTAQFA
                     ENHELFPPLLPIVGAEPAQRFGCLRLHNGTIWRWNRLLVGFDDDGQPHLRIEQRVMPA
                     GPSTIDMFANAALYYGAVHMLARRPVKVDHALPFAAARDNFYAAARHGLDAELAWLDG
                     QRLPARKVLASLIALAREGLERLGVDAALVDRYLDLVAGRLHSGRTGAAWQLAHHARH
                     GDLFRLTADYLENQRSGAPVHEWAL"
BASE COUNT          538 a         1123 c         1203 g          609 t
ORIGIN      
        1 gatgccgact ggatggcgat caagccgatg aactgtccgg cgcacgtgct gatcttcaag
       61 caggggatca agagctaccg cgacctgccg ctgcggctgg tcgaaaacgg ctgctgccac
      121 cgcaacgaac cgcacggcgc gctgcacggg atcatgcggg tgcgccagtt cacccaggac
      181 gacgggcata tcttctgccg cgaagaccag atcgtttccg aagtgcgcag cttctgccag
      241 ctggccgacc gggtctacaa ggacttcggc ttcacctatg cggtcaagct ggcgctgcgc
      301 ccggccaagc gcttcggttc ggaagccgat tgggacaagg ccgaacagga actgcgccag
      361 gcggtgatcg atgccggtct cgccaccgag gagtatggct gggaagaact gcccggcgaa
      421 ggcgccttct atgcccccaa gctcgaatgg cacctgaccg acgcgatcgg gcggacctgg
      481 caggtcggca ccatgcaatc cgaccgggtg ctgccgcagc ggctcgatgc gagctacgtt
      541 ggcgaggacg gcgagcgcca ccgcccggtc atgctgcacc gcgcgatctt tggttcctac
      601 gaacgcttca tcggcatcct gatcgaacac tatgccggca agctgccggt gtggctggcg
      661 ccggtacagg cggtggtggc gacgatcgtt tcggatgccg atggctatgc cgcagaggtt
      721 acccgtcagc tggccgaagc tggaatccgg gtcgaaaccg acgtgcgcaa cgaaaagatc
      781 aactacaagg tgcgcgatca ttcgctcaag aaggtcccgc acctcctggt ggtgggcaag
      841 cgcgaggccg aggaaggcac cgtcgcgctg cgcacgctgg gcgctgaaca ccagaaggtg
      901 atgccgctgg ccgaggcgat cgccttgctc agggccgaag caactccgcc ggacttgcgt
      961 tgacttgatc gccggtcttt cgcccgagca gatcgccatc gcgctcgccg cggcgctggc
     1021 cgcggccttc gtgcgggggc tggcgggctt tggcatggcg atcctgctgg tgccggtgct
     1081 ggggctggcg atccccccgg ccgaagcggt agtgaccggc aactggctgg ggatcttcat
     1141 cggcctggtg ggcctgcgtc agattctgag cgcgagcgag cgatcggctt tcatcatctg
     1201 cggcttcgcg atggcggcaa ccccgctggg cgtatggctg ctggcccaga ccgatccggc
     1261 catggcccgg ctgctgatcg cgctggtggc gctgggtgcc ttcgtgatgg tcctgctgcc
     1321 caagcgcgac gagggccacc aaccatcggg aatcgaaacc ggcgccaccg ggatcgcctg
     1381 cggggtgctg accggctttg ccgggatgcc cggggtgccg gtggtgccgt tctatctgcg
     1441 tcggcacctc gcgccggttg tcgcgcgtgc ctcgatgatg acgatcttcc ttgccacctc
     1501 ggttgccggg gtgggatcgg ccgtggtctt gcgcgtggca accgtgcgcg aactggtgct
     1561 ggggctggtg ctgtttcccg cggtgctgct gggcaactgg ctgggacaca gggccttcgg
     1621 gcggatcagc gatactgcct ggcgcagctt tgtcggactg gtgctggggc tttcggcgct
     1681 ggccgcgctg tggcggctgt tacacgccta gcgctggcaa ggcagggccg gccaggatca
     1741 gggccagcgc gcagccatag accaccagcg ggcgcagcac cgcgatcggg ggcaggctgg
     1801 tctgggcggc aaaggcaata gcgcgggcca tgaccctatt gtccgctgtt tgtcttaccg
     1861 aagcgtaaat tgatcgagat cgttgtcgcg gcctgctttg cgctggcacg aaggttccag
     1921 ggcaactggc cggaaagggg cagggcatgg ggcaggagat cgccacaacg ggcttttgcg
     1981 aaggtgactt tgcgcgcttt tcagcgcggc tcggcgccga aaccgcgctg ctgcgcgacc
     2041 tgtgggccgg gcacggattg tcccgccagg ggccgatcgc cgggttcgag cttgaggcct
     2101 ggctgatcga tcgcaactgt tttcccgccc cgcacaacca ggccttcctg gcgcggctgg
     2161 ccgatccgca ggtggtgccc gaactgtcgc gcttcaatat cgaactgaac ggcgatcccc
     2221 agccgctggc gggcgatggg ctggcccggc ttgaacaggc gctggggcac agctggcagc
     2281 gctgcgtggc caatgcccac gccgatgtcg ataccgcaat cgcgatcggg acgctgccca
     2341 cgctgcgcga aagcgatctg tcgctggcga tgatgacccc gtcgaaccgc tatgccgcgc
     2401 tcaatgccga actggcgcgc cagcgcggcg gcaagccggt gcggatcgac attgccagcg
     2461 cctttgccgg gggcgagcat ctggtcagcg tgcatgacga tgtcatgctc gaagccgcca
     2521 ccacctcgtt ccagctgcat cttcaggttc ccgcagacga gatcgccgcc acgctcaacg
     2581 cctcgcttgc gctctcggcg ccgctgctgg cgatcagtgc caattcgccg ttcctgttcg
     2641 gcaaggcact gtggcacgaa acccggatcg cgctgttcga ggccgcgttc gaacaggctc
     2701 cgatcaacgg cagcctgcgc cgggtcagct tcggcagcgg ctggtgcggc accgatccga
     2761 ccgcgcagtt cgcggaaaac cacgaactgt tcccgcccct gctgccgatc gtcggcgctg
     2821 aaccggcgca gcggttcggc tgtctcaggc tgcataacgg cacgatctgg cgctggaacc
     2881 gcctgctggt gggcttcgac gatgatggcc agccgcacct tcggatcgag cagcgggtga
     2941 tgcccgccgg gccgagcacg atcgacatgt tcgccaatgc cgcgctctat tacggcgcgg
     3001 tgcacatgct ggcgcgccgc ccggtcaagg ttgaccatgc cctgcctttt gccgcggcgc
     3061 gcgacaattt ctatgccgcc gcgcgccatg gccttgatgc cgaacttgcc tggctcgatg
     3121 gccagcggct gccggcccgc aaggttctgg ccagcctgat tgcgctggcc cgcgaagggc
     3181 tggagcggct gggggtcgat gcggcgctgg tcgatcgcta cctcgatttg gtggccgggc
     3241 ggctccattc agggcggacc ggcgcggcct ggcagcttgc ccatcatgcc cgccacggcg
     3301 atctgttccg gctgaccgcc gattacctcg aaaaccagcg cagcggcgcg ccggtacacg
     3361 aatgggcgct ttagctagta gagctgggca agcacggcgc cgattgcaat cgccacgccg
     3421 aagggcagct tgtcggcatc ggcctcggcc ttgcgtggca ccggattgcc cga
//
LOCUS       DASUDV010000315         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1092649,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000315 DASUDV010000000
VERSION     DASUDV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092649"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFV30_06865"
     CDS             <1..581
                     /locus_tag="VFV30_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX4847848.1"
                     /translation="ALAEALAGEWAAQGETIDPAGFVLRDLADFALDVVAGEPAPTRA
                     ALLRYAETDTLCYRADPDEPFHARQVAEWEPLVQAAEARWDVRFVRVSGIIHRPQPAE
                     TLQRMEAVLAARDDFTVAALTTLASLSASLIVALTALEPGADPQALWQAAELEALWQA
                     EQWGKDDEAEARHARRFASFAAAVRFAGLAAN"
     gene            complement(588..1193)
                     /locus_tag="VFV30_06870"
     CDS             complement(588..1193)
                     /locus_tag="VFV30_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX4847849.1"
                     /translation="MARTLLRTAALAALPLALSACVSLGGGGGKPPASLLTLTPAESA
                     PAGSAASASAAETLMVLEPETDQSLAVLRVPVQVDDANIAYLQGAVWVERPSRLFRAL
                     LAETLRAKTGRLVLEDSQATVPASGTRLSGRLIAFGYDARSGSVVVRYDAIRTGPKGE
                     VQTKRFESVVPGVAPTAAAVGPALNKAANDVAGQVAAWMAG"
     gene            complement(1193..2161)
                     /locus_tag="VFV30_06875"
     CDS             complement(1193..2161)
                     /locus_tag="VFV30_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX4847850.1"
                     /translation="METRANHVWVGAVTLGLLALIAAVILWIARINDGGRHEYDIFFK
                     QSVDGLSRGSGVTFSGVPVGQVVEIELWKKDPSFVRVRIAVEKGSPIQLGTTATIQGS
                     FTGVSNIQLEGASKDAPVLSCKGELAQACPEGVPVIPTKRGGLGELLNSAPVLLERLA
                     TLTERLSQVFSDKNLAAIDGILANTNRMTGGLADATPEFRATLMELQGTLVDARKALA
                     AFESTTMATNETLNGEGKAMLVQFRQSLKSIETVANQLNGLVGDTRPAARQLQANTLP
                     QAEAAIRDLRASTKALRDLTEKLTNEGAGSLLGGQRLPDYKPGRNR"
     gene            complement(2171..>2840)
                     /locus_tag="VFV30_06880"
     CDS             complement(2171..>2840)
                     /locus_tag="VFV30_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4847851.1"
                     /translation="MRAIIGLQVPEAGRIDVLGDNITAARDDDDIDIRSRWGVLFQGG
                     ALFSTLTVAENIEVPLKEFYPEIGPELRREIARYKVMLSGLPDEAADKYPSELSGGMK
                     KRAGLARALAMDPELLFLDEPTAGLDPIGAAAFDKLTRELQETLGHTVFLITHDLDTL
                     YEICDRVAVLADKRVIAVGTIPELLALDHPWIQEYFNGPRGRAASAARSRAKAMDKPV
                     GRGA"
BASE COUNT          398 a          988 c          978 g          476 t
ORIGIN      
        1 gcgccctggc cgaggccctg gccggcgaat gggccgcgca aggcgagacg atcgatcctg
       61 ccgggttcgt gctgcgcgat ttggccgatt tcgcgctgga cgtggtcgcg ggtgagcctg
      121 cgccgacccg cgccgcgctg ctgcgctatg ccgaaaccga cacgctgtgt taccgcgccg
      181 atccggacga gccgttccac gcgcgccagg ttgccgaatg ggagccgctg gtgcaggccg
      241 ccgaagcgcg ctgggacgtg cggttcgtgc gggtttcggg gatcatccac cgcccccagc
      301 cggccgagac gctgcagcgg atggaagcgg tgctggcggc gcgggacgat ttcaccgtgg
      361 cggcgctcac caccctcgcc agcctgtccg cctcgctgat tgtcgccttg accgcgcttg
      421 agccgggagc cgatccgcag gcgctgtggc aagcggccga gttggaagcg ctgtggcagg
      481 ccgaacagtg gggcaaggac gacgaagccg aagcccgcca cgcgcgccgc tttgcaagct
      541 ttgccgcggc ggtgcggttt gccggactgg ccgcgaactg aacggcgcta gccggccatc
      601 caggccgcga cctggccggc gacatcgttg gcggccttgt tgagcgccgg accaacggcg
      661 gcggcagtgg gtgccacgcc ggggaccacg ctttcgaacc gcttggtctg gacctcgccc
      721 ttggggccgg tgcggatcgc gtcatagcgc accaccaccg atccgctgcg cgcgtcataa
      781 ccaaacgcga tcagccgccc ggacaggcgc gtgcccgatg ccgggaccgt ggcctggctg
      841 tcttccagca ccagccgccc ggtcttggcg cgcagagttt cggccagcag cgcgcggaac
      901 aggcgcgaag gccgctccac ccagaccgcg ccctgcagat aggcgatgtt ggcatcgtcg
      961 acctgcaccg gcacccgcag cacggccagc gactggtcgg tttccggttc cagcaccatc
     1021 agcgtttcgg ccgcactggc gctggcggcg gagccggccg gggcgctttc cgccggggtc
     1081 agggtcaaga gcgaagccgg cggcttgccc ccgcctccgc ccaggctgac gcaggcggac
     1141 agggccagcg gaagcgcggc cagcgcggcg gtgcgcagca gggtgcgggc cattatcggt
     1201 ttctcccggg cttgtaatcg ggcagccgct ggccgcccag cagcgatccc gcgccttcgt
     1261 tggtgagctt ttcggtgagg tcgcgcagcg ccttggtgct ggcgcgcaga tcgcggatcg
     1321 ccgcttcggc ctggggcagg gtgttggcct gcaattgccg cgccgccggg cgggtatctc
     1381 cgaccagccc gttcagctgg ttggccacgg tctcgatcga tttcagcgac tggcggaact
     1441 ggaccagcat ggccttgccc tcgccgttca gcgtctcgtt ggtcgccatg gtggtgcttt
     1501 cgaaggcggc cagcgccttg cgcgcgtcca ccagcgtgcc ttgcagctcc atcagggtgg
     1561 cgcggaattc cggcgtggca tcggccagcc cgccggtcat ccggttggtg ttggccagga
     1621 tcccgtcgat cgcggccagg tttttgtcgg aaaagacctg gctcagccgc tcggtcagcg
     1681 tggccagccg ttccagcagg accggcgcgg aattgagcag ttcgcccagg cccccgcgct
     1741 tggtcgggat caccggcacg ccttcagggc aggcctgggc cagttcgccc ttgcagctca
     1801 gcaccggggc gtccttgctg gcgccttcga gctggatgtt ggaaacgccg gtgaagctgc
     1861 cctggatcgt ggcagtggtg ccgagctgga tgggcgagcc cttttccacc gcgatccgca
     1921 cccggacgaa gctggggtcc ttcttccaca gctcgatctc caccacctgg cctaccggga
     1981 cgcccgagaa ggtcaccccc gaaccgcgcg acaggccgtc gaccgattgc ttgaagaaga
     2041 tgtcgtattc gtggcgtccg ccgtcgttga tccgggcgat ccacaggatc accgcggcga
     2101 tcagcgccag cagccccagg gtgaccgccc ctacccagac gtgattggct ctggtctcca
     2161 tcgctgctgc ttatgccccc ctgcccaccg gcttgtccat cgccttggca cgggaccgcg
     2221 cggcgctggc cgcacggccg cgcgggccgt tgaagtattc ctggatccac gggtgatcca
     2281 gcgcgagcag ttccggaatg gtccccaccg cgatcacccg cttgtcggcc agcaccgcca
     2341 ccctgtcgca gatctcgtac agcgtatcga gatcgtgggt gatcaggaac accgtgtggc
     2401 ccagcgtttc ctgcagttcg cgggtcagct tgtcgaaagc ggccgcgccg atcgggtcga
     2461 gcccggcggt cggctcgtcc aggaacagca gttccggatc catcgccagg gcccgggcca
     2521 ggcccgcgcg cttcttcatc ccgccggaca gttcggacgg gtacttgtcc gccgcctcgt
     2581 ccggcaggcc ggagagcatc accttgtaac gggcaatctc gcgccgcagc tcggggccga
     2641 tttcgggata gaattccttc agcggcactt cgatgttttc ggccacggtc agggtcgaga
     2701 acagcgcccc gccctggaac agcacgcccc agcggctgcg gatgtcgata tcgtcatcgt
     2761 cgcgcgcggc ggtgatgttg tcgcccagca cgtcgatccg cccggcttcg ggcacctgca
     2821 gcccgatgat cgcgcgcatc
//
LOCUS       DASUDV010000316         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1932083,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000316 DASUDV010000000
VERSION     DASUDV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932083"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFV30_06885"
     CDS             complement(<1..317)
                     /locus_tag="VFV30_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4847852.1"
                     /translation="MTEARDLKDTVFLPKTEFPMKAGLPQKEPGILARWNEQGLYRKL
                     RDARAGREKFILHDGPPYANGDMHIGHALNHILKDMVCRTQNLAGKDAPYVPGWDCHG
                     LPI"
     gene            complement(373..1341)
                     /locus_tag="VFV30_06890"
     CDS             complement(373..1341)
                     /locus_tag="VFV30_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4847853.1"
                     /translation="MKLIAQRGVAQQFERKGVTENSCTATRIEKPAHDFVENTVTSLQ
                     QATRLGANMVLVDVTATADGRLVLFRDWSLDCRTDGKGPVAKLPLSDVQMLDAGYGYT
                     ADGGRSFPLRYNQTARIPTVEEAVAGLPSTPILFSLKGRTAADGALLVKALKAAGRDP
                     AKRGDGFTAPDEAALAPLRQAFPGAWSFSPQSARECASRYKLMGWFTLVPEACKNGAI
                     FVPLDGQWAYPGWPDRMLDRMAKANVRIILTAPRGEGEGQAGLDLPEQIAQVPFSYTG
                     YVWVDDIWTMGPAFQPAANRRGPEAEFTLQSNHTARRKARGLPEPQ"
     gene            complement(1450..>2223)
                     /gene="ribF"
                     /locus_tag="VFV30_06895"
     CDS             complement(1450..>2223)
                     /gene="ribF"
                     /locus_tag="VFV30_06895"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEX4847854.1"
                     /translation="ATFDPHPVRYFAPDAPPFRLTTLDQRQELFEAAGADAMLVFAFD
                     GELAGTTAEAFVTALLAGRLGAAGVVTGEDFTFGKGRGGNVRVLAELGAAAGMRARAV
                     GPVSEDGAVVSSSRIREALKSGDCGAATHLLTRPFAIRGVVEHGAKLGRTIGYPTANL
                     RLGSYLRPAYGIYAVTGVLPGGRVVHGAANLGIRPSFDPPIELLEPYFFDFSGDLYGQ
                     EIEVALHHYLRPEAKFDSLEALTAQMEEDCKTARQLLGG"
BASE COUNT          339 a          754 c          771 g          359 t
ORIGIN      
        1 tcgatcggca ggccgtggca atcccagccg ggcacatagg gcgcgtcctt gccggccagg
       61 ttctgggtgc ggcagaccat gtccttcagg atgtggttca gcgcgtggcc gatgtgcatg
      121 tcgccgttgg catagggcgg gccatcgtga aggatgaact tctcgcgccc ggcgcgggca
      181 tcgcgcagct tgcggtacag gccctgctcg ttccagcgcg ccagaatgcc cggctccttt
      241 tgcggcaagc cggccttcat cgggaactcg gtcttcggca ggaagacggt gtctttaaga
      301 tcgcgcgctt cagtcatagc gcgcgggcct tagcgggccg cagcgccaag cgcaatttct
      361 gcgtcgcagg gcttactggg gctcgggcag gccgcgggcc ttgcgccggg cggtgtggtt
      421 gctctgcagc gtgaattcgg cctccggccc gcggcggttg gcggccggtt gaaaggccgg
      481 gcccatggtc cagatgtcat ccacccagac atagccggtg tagctgaagg gcacctgggc
      541 gatctgttcg ggcaggtcga gcccggcctg gccctcgcct tcgccgcgcg gcgcggtcag
      601 gatgatccgg acattggcct tggccatccg gtccagcatc cggtcgggcc agccgggata
      661 ggcccattgg ccgtcgaggg gcacaaagat cgccccgttc ttgcaagcct cgggcaccag
      721 ggtgaaccag cccatcagct tgtagcggct ggcgcattcg cgcgcgcttt gcggcgagaa
      781 cgaccaggcc ccggggaagg cctggcgcag cggagccagc gcggcctcgt ccggcgcggt
      841 aaagccgtcg ccgcgcttgg cgggatcgcg cccggcggct ttcagcgctt tcaccagcag
      901 cgccccatcg gcggcggttc ggccctttag cgagaacagg atcggggtgc tgggcaggcc
      961 ggccacggcc tcttccaccg tggggatccg cgcggtctgg ttgtagcgca gcgggaagct
     1021 gcgcccgccg tcggcggtat agccatagcc ggcatccagc atctgcacgt cgctgagcgg
     1081 cagtttggcg accgggccct tgccatcggt gcggcagtcc agcgaccagt cgcggaacag
     1141 caccagccgc ccatcggcgg tcgcggtcac gtcgaccagc accatgttcg cgcccagccg
     1201 ggtggcctgc tgcaggctgg taacggtgtt ctcgacaaaa tcgtgcgccg gcttctcgat
     1261 ccgggtggcg gtgcagctgt tctcggtcac gcccttgcgt tcgaactgct gggccacgcc
     1321 gcgctgggcg atcagcttca tgtatccgcg cggcgccggg gccagccaac tggcattcgc
     1381 ggcaaagacc cccacaagaa ccagtccgac accgctgaac gcccaactga cccaaggttt
     1441 catgccgaac tatcccccca acagctgccg cgccgtcttg cagtcttctt ccatctgggc
     1501 ggtaagcgct tccaggctgt cgaatttcgc ttccggccgc aggtaatggt ggagcgcaac
     1561 ttcgatctct tgtccataga gatcgccgga aaaatcaaag aaatagggtt ccagcagttc
     1621 gatcggcgga tcgaaactgg ggcggatgcc caggttggcc gcgccgtgga ccacccgccc
     1681 gccgggcagc accccggtga cggcatagat cccataggcc ggccgcaggt aagagccgag
     1741 ccgcaggttg gcggtgggat agccgatggt ccggcccagc ttggcgccgt gctccaccac
     1801 gccccggatt gcgaagggcc gggtcagcag atgggttgcc gcgccgcaat cgccgctttt
     1861 cagcgcctcg cggatccggc tggaggagac gaccgcgccg tcctcgctga ccgggccgac
     1921 cgcgcgggcc cgcatgccgg ccgccgcgcc cagctcggcc agcacccgca cgttgccccc
     1981 gcggcccttg ccgaaggtga aatcctcgcc ggtgaccact ccggccgcac ccagccggcc
     2041 ggccagcagg gcggtgacga aggcttcggc cgtggtcccg gccagctcgc cgtcgaaggc
     2101 gaagaccagc atagcgtcgg ccccggcggc ctcgaacagc tcctgccgct ggtccagcgt
     2161 ggtcaggcgg aacggcgggg catcgggggc gaaatagcgc accgggtggg gatcgaaagt
     2221 ggc
//
LOCUS       DASUDV010000317         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_252923,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000317 DASUDV010000000
VERSION     DASUDV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252923"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFV30_06900"
     CDS             <1..661
                     /locus_tag="VFV30_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4847855.1"
                     /translation="DDVMLNRRDAQALIPVVTGKQPLYVRVERAADIRMVLTLKKEFP
                     AVRLVIVGATEGWMVAGELARSGVPVLATPLNNLPDTFEQLGATQSNVGRMKAGGVKV
                     SLGAFFDQPRYAPQYAGNLVAVGRVPGASGLTWNQAFSAISSGPAEAMGLGDRFGSLR
                     PGRAGDVVIWDGDPLEVTSGVTMVFIDGVEQPLKDHQTRLRDRYKSGPAEGALPKAYE
                     W"
     gene            813..1493
                     /locus_tag="VFV30_06905"
     CDS             813..1493
                     /locus_tag="VFV30_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX4847856.1"
                     /translation="MDAPLANLIAAATAFVGTHFALSHPLRAPLVRITGEKAFPGLYS
                     LVAFATFGWLIMAFRAVAPAAPLWDGMGTAPWIAASVLMLVASVLLAGSFIGNPAMPA
                     PGAAAAAARGPHGVFHVTRHPMMWSFALWSAAHALLSPTPRVLVLTLAVAILALVGSH
                     LQDRKKAVLMGPAWQGWQAQTAYWPRLTALGKAGAVPWLGGTALWLAASWSHIPLAAI
                     PAGVWRWL"
     gene            complement(1495..2295)
                     /locus_tag="VFV30_06910"
     CDS             complement(1495..2295)
                     /locus_tag="VFV30_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847857.1"
                     /translation="MTLRRLLLAAAVLLGLYAAGLGALYAGQRSLIFPAPQGPVPLPS
                     GYSAVALKTADGLSLKAFYRPPAAGQPTLVFFHGNGDSLNGSLVAVERYAAAGYGVLL
                     PEYRGYGGNPGSPSEEGLYADGRAARAWLAQQGVAPARQVLIGFSLGTGVVTRLAEEQ
                     PPAALILIAPYTSIPDVVTHRFAGLVPGALARDRLDSLSRIDRIAAPLLILHDRDDLS
                     IPVSHGQRLAERSGRAKLVLFSGHGHQLGFAGEAQEAGLSWLAGLGLR"
     gene            complement(2292..2795)
                     /locus_tag="VFV30_06915"
     CDS             complement(2292..2795)
                     /locus_tag="VFV30_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847858.1"
                     /translation="MNRPLRRLLPTLLVAAVAFGLWQLAASFTVAADLARILLFAAAG
                     IGLVAVLEFRGAGNMKGCMTFMALLLLPAFGLAMAGVDRLAAMPELDPLVGWAGGFRV
                     PAFLAVAAGALGFVFVARQKFFGKSRIRISSYTETALFTLFFALVGGVTGLVWMALDY
                     GLRQVGL"
     gene            complement(2792..3343)
                     /locus_tag="VFV30_06920"
     CDS             complement(2792..3343)
                     /locus_tag="VFV30_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847859.1"
                     /translation="MAEAAAAPFEARYSVSKMLVWTLLAGIMTAGALGMLLPALEQGS
                     LGGGVIGGCGLVVFGGALLGFLRCAVDRRVQLRIDGKGMYLRPHSDQTIPLRSIRGVE
                     VAPGQVKVRLYKPSKYPIQSRLRRFIFRINGSASREYFGDAWVWTAHYDCTWQQIMDA
                     INVHTVPTEFERQLAERMKAGHE"
     gene            complement(3346..>5101)
                     /gene="dxs"
                     /locus_tag="VFV30_06925"
     CDS             complement(3346..>5101)
                     /gene="dxs"
                     /locus_tag="VFV30_06925"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX4847860.1"
                     /translation="ELTVAIHYVFNTPDDKLVWDVGHQAYPHKIITGRRDRIRTLRQG
                     GGLSGFTKRSESEYDPFGAAHSSTSISAALGFAIANKLAGTPGKGIAVIGDGAMSAGM
                     AYEAMNNAAEAGNRLVVILNDNDMSIAPPVGGLSAYLARLVSSRPFLSLREIAKRLSR
                     KLPEPLHRAAKKTDEFARGMAMGGTLFEELGFYYVGPIDGHNLDQLIPVLENVRDAAE
                     GPCLIHVVTQKGKGYAPAEASADKYHGVQKFDVITGQQVKPAASAPAYQNVFGETLAK
                     LADTDPRICAITAAMPSGTGVDKFAKAHPDRAFDVGIAEQHGVTFAAGLAAQGMRPFC
                     AIYSTFLQRAYDQVVHDVAIQNLPVRFAIDRAGLVGADGATHAGSFDITYLASLPNMV
                     VMAAADEAELVHMTYTAALHDSGPIAFRYPRGNGVGVALPEVPQALEIGKGRIVRQGT
                     KVAILSLGTRLAEALKAADQLEAKGLSTTVADLRFAKPLDTDLIRQLMTTHEVVLTVE
                     EGSIGGLGAHVLTMASDEGLTDAGLKIRTLRLPDTFQDHDSPDKQYDAAGLNAPHIVD
                     AVLKALRHNSAGVEEARA"
BASE COUNT          804 a         1761 c         1762 g          774 t
ORIGIN      
        1 cgacgacgtc atgctcaacc gccgcgatgc ccaggcgctg atcccggtcg tcaccggcaa
       61 acagccgctc tacgtgcggg tcgagcgcgc ggccgacatc cgcatggtcc tgacgctcaa
      121 gaaggagttc ccggcggtcc ggctggtgat cgtcggcgcg accgagggct ggatggtggc
      181 gggcgaactg gcccgctccg gcgtgcccgt tctggccacc ccgctcaaca acctgccgga
      241 caccttcgaa cagcttggcg cgacccagtc caacgtcggg cggatgaagg ccggcggggt
      301 caaggtctcg ctcggcgcgt tcttcgatca gccgcgctat gccccgcaat acgccggaaa
      361 cctggtggcg gtcggccgtg tgccgggcgc cagcgggctg acctggaacc aggccttcag
      421 cgcgatcagc tctggcccgg ccgaagcgat ggggctgggg gaccggttcg gatccctccg
      481 cccgggccgc gccggcgacg tggtgatctg ggacggcgat ccgctggaag tgaccagcgg
      541 cgtgaccatg gtgttcatcg acggcgtcga acagccgctg aaggaccacc agacccgcct
      601 gcgcgaccgc tacaagagcg gcccggccga aggcgcgctg cccaaggcct acgaatggta
      661 agggcccccg cgccgcctgg cgccatctga ggccgaagcc catcacaagg ccgtcaccgg
      721 gagaacgccc ggtggcggcc tttgtgtttg catccgcccg aaagtgtgct agccatgttt
      781 acgccgtaaa cataagggca gggagaaccc cgatggacgc accgctcgcc aacctgatcg
      841 ccgccgccac cgcctttgtc ggcacccact ttgccctgtc gcacccgctg cgcgcgccgc
      901 tggtccggat caccggggaa aaggctttcc ccgggctcta ttcgctggtc gcctttgcca
      961 cctttggctg gctgatcatg gcgttccgcg ccgtggcgcc ggccgcgccg ctgtgggacg
     1021 ggatgggcac cgcgccctgg atcgccgcca gcgtcctgat gctggtcgcc tcggtcctgc
     1081 tggccggatc gttcatcggc aacccggcca tgcccgcccc cggcgcagcg gccgccgccg
     1141 cgcgcggccc gcacggggtg ttccatgtca cccgccatcc tatgatgtgg tccttcgcgc
     1201 tgtggagcgc ggcccacgcg ctgctcagcc cgaccccgcg ggttctggtc ctcactctcg
     1261 ccgtggcgat cctcgcgctg gtcggctcgc acctgcagga ccgcaagaag gcggtgctga
     1321 tggggcctgc ttggcagggc tggcaggccc agaccgccta ctggccgcgc ctcaccgcac
     1381 tgggcaaggc cggggctgtc ccctggctgg gcggcaccgc gctgtggctg gcggccagct
     1441 ggtcgcacat cccgctcgcg gcaatcccgg cgggggtgtg gcgctggctc tagtctaacg
     1501 caggcccagt ccggccagcc agctcagacc ggcctcctgc gcctcgccgg caaagccaag
     1561 ctggtggcca tggccgctga acagcaccag ctttgcccgc ccggagcgtt ccgccagccg
     1621 ctggccgtgg ctgaccggga tcgacaggtc gtcgcggtcg tgcaggatca gcagcggcgc
     1681 ggcgatgcga tcgatccggg acaggctgtc gagccggtcg cgggccagcg cgccgggcac
     1741 cagtccggca aagcggtggg tcactacatc gggaatgctg gtgtagggcg cgatcaggat
     1801 cagcgccgcc gggggctgct cctcggccag ccgggtgacc accccggtac ccagcgaaaa
     1861 gccgatcagc acctgccggg cgggcgccac gccctgctgc gcgagccagg cccgcgccgc
     1921 gcggccatcg gcgtagaggc cttcctcgct cggcgaaccg ggattgccgc catagccgcg
     1981 gtattcgggc agcagcacgc cgtatccggc cgcggcatag cgttcgacgg caaccaacga
     2041 tccgttcagg ctgtcgccgt tgccgtgaaa gaacaccagg gtcggctgcc ccgcggccgg
     2101 cgggcggtag aacgccttga gggatagccc gtcggcggtc ttcagcgcga cggcgctgta
     2161 gcctgatggc aacggcacgg ggccctgcgg cgccggaaag atcaggctgc gctgcccggc
     2221 ataaagcgcg cccagccccg cggcataaag ccccagcagc accgcggcgg ccagaagcag
     2281 gcggcgcagc gtcacagccc aacctgccgg aggccatagt ccaaggccat ccagaccagc
     2341 cccgtcacgc cgccgacaag tgcgaagaag agcgtaaaca gcgcggtttc agtataggag
     2401 gaaatccgta tccggctctt tccgaagaac ttctgacggg cgacgaacac gaagcccagg
     2461 gcgcccgcgg caaccgccag gaaggcaggg acgcggaagc caccggccca gccgaccagc
     2521 gggtcaagct ccggcatcgc cgccagccgg tccacaccgg ccatggcaag gccaaaggcc
     2581 ggaagcagga gcagggccat aaaggtcatg caccccttca tgttgcccgc gccccggaat
     2641 tccaggactg cgaccaggcc aatgccagcc gccgcgaaca gcaggatccg ggccaggtca
     2701 gcggcaacgg tgaagctggc agcaagctgc cacaggccga atgccaccgc ggcgaccagc
     2761 agcgtgggca ggagccggcg gagcggccgg ttcattcgtg cccggccttc atccgttcgg
     2821 ccaactggcg ctcgaactcg gtcggcacgg tatggacgtt gatcgcgtcc atgatctgct
     2881 gccaggtgca atcgtaatgc gcggtccaga cccaggcatc gccgaagtac tcgcgagacg
     2941 cgctgccgtt gatgcggaaa atgaagcggc gcagccgact ctggattggg tactttgatg
     3001 gcttgtagag ccgcaccttg acctgtccgg gggccacttc gacgccccgg atcgagcgga
     3061 gggggattgt ctggtccgaa tggggccgca gatacatccc cttgccgtcg atccgcaatt
     3121 gcacccgccg gtcaaccgcg caacgcagga agcccagcaa ggccccgccg aaaaccacca
     3181 gaccgcaacc gccgatcacg ccgccgccga ggctgccctg ttccagcgcg ggaagcagca
     3241 tcccgagcgc gcccgctgtc atgattcccg cgagcagggt ccagacgagc atcttggaaa
     3301 ccgaatagcg cgcctcgaac ggcgcagccg ccgcctcggc catgctcagg cccgcgcttc
     3361 ctctaccccg gccgaattgt ggcgcagcgc cttcagcacg gcatcgacga tgtgcggggc
     3421 gttgaggccg gccgcgtcgt actgcttgtc ggggctgtcg tgatcctgga acgtgtccgg
     3481 caggcgcagg gtgcggatct tcaggccggc gtcggtcagg ccttcgtcgc tcgccatggt
     3541 cagcacgtgg gcgcccaggc cgccaatgct gccttcctcg accgtcagca ccacttcgtg
     3601 cgtggtcatc agctggcgga tcaggtcggt atccagcggc ttggcaaagc gcaggtcggc
     3661 caccgtggtg gacaggccct tggcctccag ctggtcggcc gccttcagcg cttcggccag
     3721 gcgcgtgccg agcgagagga tcgccacctt ggtgccctgg cgcacgatcc ggcccttgcc
     3781 gatttccagc gcttgcggaa cttcgggcag ggccacgccc accccgttgc cgcgcggata
     3841 gcgaaaggcg atcggaccgc tgtcatgaag cgcggcggta taggtcatgt ggaccagctc
     3901 ggcctcgtcc gcggcggcca tgacgaccat gttgggcagg ctcgcgaggt aggtgatgtc
     3961 gaacgagccg gcatgggttg ccccgtctgc gccgaccagc ccggcgcggt cgatcgcgaa
     4021 gcggaccggc aggttctgga tcgcgacgtc atggaccacc tggtcgtagg cgcgctgcag
     4081 gaaggtggaa tagatcgcgc agaacgggcg catgccctgc gccgccaggc cggcggcaaa
     4141 ggtcaccccg tgctgttcgg caatgccgac gtcgaacgcg cggtccgggt gggccttggc
     4201 gaacttgtcg accccggtgc cgctgggcat ggcggcggtg atcgcgcaga tccggggatc
     4261 ggtgtcggcc agcttggcaa gcgtctcgcc gaagacgttc tgataggccg gggcgctggc
     4321 cgcgggcttg acctgctggc cggtgatcac gtcgaacttc tgcacgccgt ggtacttgtc
     4381 ggcgctggct tcggccgggg catagccctt gcccttctgc gtgacgacat ggatcaggca
     4441 cggcccttcg gcggcgtcgc ggacgttttc cagcaccggg atcagctgat ccaggttgtg
     4501 gccgtcgatc gggccgacgt agtagaagcc cagctcctcg aacagggtgc cgcccatcgc
     4561 catgccgcgg gcgaactcgt cggtcttctt ggcggcgcgg tgcagcggtt cgggcagctt
     4621 gcgcgacagc cgcttggcaa tttcgcgcaa ggacaggaac gggcgcgagg agaccagccg
     4681 cgccagatag gccgaaagcc cgcccaccgg cggcgcgatc gacatgtcgt tgtcgttcag
     4741 gatcacgacc agccggttgc cggcttcggc cgcgttgttc atcgcctcat aggccatgcc
     4801 ggcgctcatc gcgccgtcgc cgatcacggc gatgcccttg cccggggtgc cggccagctt
     4861 gttggcgatg gcaaagccca gcgcggccga gatcgaggtg gagctgtgcg ccgcgccgaa
     4921 cggatcgtat tcgctctcgc tgcgcttggt gaagcccgaa aggccgccgc cctggcgcag
     4981 ggtgcggatc cggtcgcgcc ggccggtgat gatcttgtgg ggataggcct ggtggccaac
     5041 gtcccagacc agcttgtcgt ccggcgtgtt gaagacatag tggatcgcga cggtcagttc
     5101 g
//
LOCUS       DASUDV010000318         5658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1070893,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000318 DASUDV010000000
VERSION     DASUDV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070893"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFV30_06930"
     CDS             <1..474
                     /locus_tag="VFV30_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX4847861.1"
                     /translation="MASPDDCALALRGLRTMGVRLERSSATAITVAEWLQGREEIGAV
                     LFPMLPGAPGHDLWKRDFTGGCGLMTIIMAGKDLKARDAFIDALSLFGIGYSWGGFES
                     LALGSHPERDRDVMPWPPRGIAPENRHAVRLWIGLEDPADLIADLERGLAAWDAA"
     gene            471..1307
                     /locus_tag="VFV30_06935"
     CDS             471..1307
                     /locus_tag="VFV30_06935"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX4847862.1"
                     /translation="MSWPEFLGWLDNIALDVGGYHISLLGGLKVITVAVLVLIAGWLL
                     TRLVRRFFRRLDRLDPARQVLGEKLVGVVVWTALVLIGIDALGISLTALTVFSGAFGL
                     AIGFGLQKTLGNLISGIILLMDRSIEPGDVIAVGSGSDKTVGKVDRIGMRAVAVTTRD
                     RIKYMIPNELLMTSAVENWTASTKNERMKVAVRVAYGTDLELAERLMLQATEGVERVQ
                     SEPKPAVWLVAFAETAIEYEIQVSISDPELGNGGIRSDILGKIWRLFRDHGIALPAPP
                     DA"
     gene            1384..2157
                     /gene="cobA"
                     /locus_tag="VFV30_06940"
     CDS             1384..2157
                     /gene="cobA"
                     /locus_tag="VFV30_06940"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX4847863.1"
                     /translation="MSIASSKPGKVWLVGAGPGDPDLLTLRAARLIMRAALIVHDGLV
                     DPAILALAGPKARLISVAKSRSRHTLQQDEINALLVREALAGSDVVRLKGGDPFIFGR
                     GGEEAEACRAAGVEVEVVPGISAAIGAAAAAQMPLTHRKSASIVSFVAGQCKGLSEQD
                     WTGLAGKGRTLVIYMGLATADAIAEKLIADGLAPDVPVAVVENATRPAMRILRSPLAG
                     LGDLVARHKVKSPALIVIGEVAAEPADEPLKTLALEAFQ"
     gene            2154..2450
                     /locus_tag="VFV30_06945"
     CDS             2154..2450
                     /locus_tag="VFV30_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEX4847864.1"
                     /translation="MKILTGNDLKTGAVTWWDGVQWSLHVEDAVDVGEHADTILAREQ
                     AARRVNAAYAIEAVKDEGGVRPAHIKDRIRALGPTVRPDLTLKPADPTAGDWVI"
     gene            2450..4078
                     /locus_tag="VFV30_06950"
     CDS             2450..4078
                     /locus_tag="VFV30_06950"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX4847865.1"
                     /translation="MYKYDTYDQAMVDARVEEFRDQVRRRLEGHITEDQFKPLRLMNG
                     LYLQLHAYMLRVAIPYGTLNSAQMRMLGDIADKYDRGYGHFTTRQNIQYNWIKLEDAG
                     DILADLAKVEMHAIQTSGNCIRNTTSDQYAGAAADEVVDPRPYAELIRQWSTFHPEFS
                     FLPRKFKMAVIASDTDRAAMKLHDIGIQIVRNDADEIGAAFYVGGGMGRTPMIAPCIN
                     PFVPIDRMITYAEACLRVYNRYGRRDNKYKARIKILVHELGKDEYTRQVEAEFAHLLG
                     QNIEPPLAELERIKAMFADPPFEVGIADEVDRSDPDFALWVDTNCLAHKQPGYIAATI
                     SLKPVGGIPGDATGEQIRLMADLAKQYSFDELRVTHAQNIVLPHVKKADLYALWQVLD
                     ANGLGTANLDLVGDIIACPGLDYCSLANARSIPVAQKISQRLSDKAQDIGELKLKISG
                     CINACGHHHAGHIGILGVDKKGVENYQLLLGGCEGADTSLGQITGPGFSEDGIVDAVE
                     KATEVYLANKQQGERFLDTYRRIGMAPFKEAIYE"
     gene            4071..4499
                     /locus_tag="VFV30_06955"
     CDS             4071..4499
                     /locus_tag="VFV30_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX4847866.1"
                     /translation="MSDVQFRFRSDEPVADPAVTVDSFGDQSNASAVRLEPGDDARDL
                     IPHLSRLQLIEVNFPVFGDGRGYSAARILREAGYQGELRAVGEVLVDQIAFMRRCGFD
                     AFAPEVPLEPADAEAAFARYDHVYQPTTDGQVPIWARRHA"
     gene            4492..5277
                     /locus_tag="VFV30_06960"
     CDS             4492..5277
                     /locus_tag="VFV30_06960"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX4847867.1"
                     /translation="MPEAARRIDRLDTAPRFTAEQVAALNARFADVPTIAMLRAVLRE
                     GLAGDIAAVSSFGAESAVLLHLIATIDRSVPVLFLETGKHFPETLAYRDQIVKRLGMT
                     NLVSLTPDAAALAKRDESGLRWSYDPDGCCEIRKVQPLAAALSHFDASFTGRKGFQNA
                     ARAGLPTFELDQTDARGRLKINPLATWSGDQVRGYFEATGLPPHPLVAQGYPSIGCSP
                     CTTKVAPGEDPRSGRWKGWDKTECGIHVDGAASQLPPGFDPAF"
     gene            5362..>5658
                     /locus_tag="VFV30_06965"
     CDS             5362..>5658
                     /locus_tag="VFV30_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4847868.1"
                     /translation="MEAPVYPFQLTQSYSPAQTDEDLLDSTVGSVLRDAAALSPGSTA
                     LIEADGDGIPRRSWTYAELLADSERLARALLTRFEPGERVAVWAPNVPEWILAEF"
BASE COUNT          957 a         1940 c         1873 g          888 t
ORIGIN      
        1 atggcatcgc ccgatgattg cgcgctggcg ctgcgcgggc tgcggaccat gggcgtgcgg
       61 ctggaacgat caagcgccac ggcaatcacc gtggccgaat ggctgcaagg ccgcgaagaa
      121 attggcgcgg tgctgttccc gatgctgccc ggcgcgccgg ggcatgacct gtggaagcgc
      181 gactttaccg gcggctgcgg gctgatgacc attatcatgg ccggcaagga cctcaaagcg
      241 cgcgatgcct tcatcgacgc gctcagcctg ttcgggatcg gctacagctg gggcggtttt
      301 gaaagcctgg cgctgggcag ccatccggag cgtgaccgcg acgtgatgcc ctggccgccg
      361 cgcgggatcg caccggaaaa ccgccacgcg gtgcggctgt ggatcgggct ggaagatccg
      421 gccgacctga tcgccgatct ggagcgcggc ctggcggcct gggacgcggc atgagctggc
      481 cagaattcct cggctggctg gacaatatcg cgctggatgt cggcgggtat cacatctcgc
      541 tgctgggcgg gctgaaggtt atcaccgtgg ccgtgctggt gctgatcgcc ggctggctgc
      601 tcacccgtct ggtgcgccgc tttttccgcc gcctcgaccg gctcgatccg gcccggcagg
      661 tgctgggcga aaagctggtc ggcgtggtgg tctggaccgc gctggtcctg atcgggatcg
      721 acgcgctggg catcagcctg accgcgctga ccgtcttttc cggcgccttt ggcctcgcca
      781 tcggctttgg cctgcaaaag acgctgggca acctgatttc gggcatcatc ctgctgatgg
      841 accgctcgat cgaaccgggc gacgtgatcg cggttggcag cggcagcgac aagaccgtcg
      901 gcaaggtcga ccggatcggg atgcgcgccg tggcggtgac cacgcgcgac cggatcaagt
      961 acatgatccc caacgagctt ctgatgacct cggcggtgga gaactggacc gccagcacca
     1021 agaacgagcg gatgaaggtg gccgtgcggg tcgcctatgg caccgatctg gagctggccg
     1081 agcggctgat gctgcaggcc accgaaggcg tggaacgggt gcagagcgaa ccgaagcccg
     1141 ccgtctggct ggtcgccttt gccgaaaccg cgatcgaata cgaaatccag gtctcgatct
     1201 ccgatccgga actgggcaac ggcgggatcc gttcagacat cctgggcaag atctggcggc
     1261 tgttccgcga tcacggcatc gcgcttcccg ccccgcctga tgcgtaaacg gcgaaagctg
     1321 acctaatccc ctgcaaagcc attctggctg gcaccgcgcg ggccaagcgc gcatttcggc
     1381 cccatgagca ttgcatcctc caaacccgga aaggtctggc tggtcggcgc cggtcccggc
     1441 gatcccgatc tgctgaccct gcgcgccgcg cggctgatca tgcgcgcggc gctgatcgtg
     1501 cacgacgggc tggtcgatcc ggcgatcctg gccctggccg ggcccaaggc gcggctgatc
     1561 tcggttgcca agagccgctc gcgccacacc ctgcagcagg acgagatcaa cgccctgctg
     1621 gtgcgcgaag cgctggccgg cagcgatgtg gtccgcctga agggcggcga tccgttcatt
     1681 ttcggccgcg gcggggaaga ggccgaggcc tgccgcgccg ccggggttga ggtggaagtc
     1741 gtccccggga tcagcgccgc gatcggcgcc gccgccgccg cgcagatgcc gctgacccac
     1801 cgcaagagcg cctcgatcgt cagcttcgtc gcgggccagt gcaagggcct gtccgagcag
     1861 gattggaccg gcctggccgg caagggccgc acgctggtga tctacatggg cctcgccacc
     1921 gccgatgcca ttgccgaaaa gctgatcgcc gatggcctgg cgcccgatgt gccggtcgcc
     1981 gtggtcgaga acgccacccg cccggcaatg cgcatcctgc gcagcccgct ggcgggcctg
     2041 ggtgatctgg tggcgcgcca caaggtgaag agcccggccc tgatcgtgat tggcgaagtc
     2101 gcggccgagc ccgccgacga gcccctgaaa acccttgccc tggaagcgtt ccaatgaaga
     2161 ttctgaccgg aaacgatctg aagaccggcg ccgtcacctg gtgggacggc gtgcaatggt
     2221 cgctccatgt ggaggacgcg gtcgatgtcg gcgagcacgc cgacacgatc ctggcccgcg
     2281 agcaggccgc ccgccgcgtc aacgccgcct atgcgatcga ggcggtgaag gacgaaggcg
     2341 gagtgcgccc cgcgcacatc aaggaccgca tccgcgcgct cggcccgacc gtgcgccccg
     2401 acctgacttt gaagccggcc gatcccaccg ccggcgattg ggtgatctga tgtacaagta
     2461 tgatacctat gaccaggcca tggtcgatgc ccgcgtcgag gaattccgcg accaggtccg
     2521 ccgccggctg gaaggccaca tcaccgagga ccagttcaag ccgctgcggc tgatgaacgg
     2581 cctgtacctg cagcttcacg cctacatgct gcgggtcgcc attccctatg gcacgctgaa
     2641 ttcggcgcag atgcggatgc tgggcgacat cgccgacaag tacgaccgcg gctatggcca
     2701 cttcaccacc cgtcagaaca tccagtacaa ctggatcaag ctggaggatg ccggcgatat
     2761 cctggccgat ctggccaagg tggaaatgca cgcgatccag accagcggca actgcatccg
     2821 caacaccacc agcgaccagt acgccggcgc ggcggcggac gaagtggtcg atccccggcc
     2881 ctatgccgag ctgatccgcc agtggtccac cttccacccg gaattcagct tcctgccgcg
     2941 caagttcaag atggcggtga tcgcctctga caccgaccgc gcggcgatga agctgcacga
     3001 catcggcatc cagatcgtgc gcaacgacgc ggacgagatc ggcgcggcgt tttatgtcgg
     3061 cggcggcatg ggccgcaccc cgatgatcgc gccctgcatc aatcccttcg tgccgatcga
     3121 ccggatgatc acctatgccg aggcctgcct gcgggtctac aaccgctatg gccggcgcga
     3181 caacaagtac aaggcgcgga tcaagatcct ggtccacgaa ctgggcaagg acgaatacac
     3241 ccgccaggtc gaagccgaat tcgcccacct gcttggccag aacattgaac ccccgctggc
     3301 cgagctggag cggatcaagg cgatgttcgc cgatccgccg ttcgaagtcg ggattgctga
     3361 cgaagtggac cgcagcgatc ccgacttcgc gttgtgggtg gacaccaatt gcctggccca
     3421 caagcagccg ggctacatcg ccgcgacgat cagcctgaag ccggtcggcg gcatcccggg
     3481 cgacgccacg ggcgagcaga tccggctgat ggccgacctg gccaagcagt attcgttcga
     3541 cgagctgcgc gtcacccacg cccagaacat cgtgctgccg cacgtgaaga aggccgatct
     3601 ctatgcgctg tggcaggtgc tcgacgcgaa cggcctcggc actgccaatc tcgacctggt
     3661 gggcgatatc atcgcctgcc cggggctgga ttactgcagc ctcgccaacg cccgctcgat
     3721 cccggtcgcg cagaagatca gccagcgcct gtcggacaag gcccaggaca tcggcgaact
     3781 gaagctcaag atctcgggct gcatcaacgc ctgcgggcac caccacgccg gccacatcgg
     3841 catcctcggc gtcgacaaga agggggtgga aaactaccag ctcctgctcg gcgggtgcga
     3901 gggcgcggac acctcgctgg gccagatcac cggccccggc ttcagcgaag acgggatcgt
     3961 cgacgcggtg gaaaaggcca ccgaggtcta tctggccaac aagcagcagg gcgagcgctt
     4021 cctcgatacc taccgccgca tcggcatggc cccgttcaag gaggcgatct atgagtgacg
     4081 tccagttccg cttccgcagc gacgagccgg tggccgatcc ggccgtcacg gtcgattcct
     4141 tcggcgacca gtccaacgcc agcgccgtgc ggcttgaacc gggcgacgat gcccgcgacc
     4201 tgatccccca cctgtcccgg ctgcagctga tcgaggtgaa cttcccggtg tttggcgacg
     4261 ggcgcggcta ttccgccgcc cggatcctgc gcgaggccgg ctatcagggc gagctgcgcg
     4321 cggtgggcga agtgttggtc gatcagatcg ccttcatgcg ccgctgcggg ttcgatgcct
     4381 tcgcccccga agtgccgctc gaaccggccg acgccgaagc ggcctttgcc cgctacgatc
     4441 acgtgtacca gcccaccacc gacggccagg tgccgatctg ggcccgccgg catgcctgaa
     4501 gccgcgcgcc gcatcgaccg cctcgacacc gctccgcgct ttaccgcgga gcaggtcgcg
     4561 gcgctcaatg cgcgctttgc ggacgtcccc acgatcgcca tgctccgcgc tgtgctgcgc
     4621 gaagggctgg cgggcgatat cgccgcggtc tccagcttcg gcgcggagag cgcggtgctg
     4681 ctgcacctga tcgccacgat cgaccgttcg gtcccggtgc tgttccttga aacgggcaag
     4741 cactttcccg aaaccctggc ctaccgcgac cagatcgtga agcggctggg catgaccaac
     4801 ctggtaagcc tgacccccga tgccgcggcg ctggccaagc gcgacgaaag cgggctgcgc
     4861 tggtcctacg atccggacgg ctgctgcgag atccgcaagg tccagccgct ggccgcggcc
     4921 ctttcgcact tcgacgccag cttcaccggc cgcaagggct ttcagaacgc ggcccgggcc
     4981 ggcttgccga cctttgagct ggaccagacc gacgcccgcg gccggctgaa gatcaacccg
     5041 ctggccacct ggagcggcga ccaggtccgg ggctacttcg aagccaccgg cctgccgccg
     5101 cacccgctgg tggcgcaagg ctatccctcg atcggctgct ccccctgcac caccaaggtc
     5161 gcccccggcg aagacccgcg ctcgggccgc tggaagggct gggacaagac cgaatgcggc
     5221 atccatgtcg atggcgcggc cagccaactg ccgccgggct tcgatccggc gttctgatcc
     5281 gcgccaattc cggcgttgca ttcgcgcgcg gcgctgtcac actccgccca cggtcgcttg
     5341 acgctacggc tttgcggccg ggtggaggcg ccggtgtatc cctttcagct gacccaatcc
     5401 tattcccccg cccagactga tgaagacctg ctggacagca cggtcggcag cgtcctgcgc
     5461 gatgcggcgg ccctatcgcc cggcagcacc gccctgatcg aggccgacgg cgacggcatt
     5521 ccgcggcgca gctggaccta tgccgagctg ctggccgaca gcgaacggct ggcccgcgcc
     5581 ctcctgaccc ggttcgagcc cggcgagcgg gtggccgtct gggcgccgaa cgtgcccgaa
     5641 tggatcctgg ccgaattc
//
LOCUS       DASUDV010000319         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1932137,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000319 DASUDV010000000
VERSION     DASUDV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932137"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /gene="argJ"
                     /locus_tag="VFV30_06970"
     CDS             <1..1164
                     /gene="argJ"
                     /locus_tag="VFV30_06970"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX4847869.1"
                     /translation="TPRVARAGYKDWGRCDLTYVELAEGTAVAGVFTRNVCCSSEVEL
                     GRANIAQGQARALVVNAGNSNAFTGYRGREAVEAIMAQVATHLGCPAEQVFVSSTGVI
                     GVPLPKDKARAGIEAALVAEPCGWEDVANTIGTTDTFAKGAMAQAVVDGRTVTLAAVI
                     KGSGMIAPDMATMLGYIFTDCAIAPGYLQELLGAANQRTFSCITVDSDTSTSDTVLAF
                     ATGMAGNRLITSADCPGADAFAAALEDVCRQLAHLVVRDGEGAQKFIAISVTGAASDA
                     SARKVGLAIANSPLVKTAIAGEDANWGRVVMAVGKAGEPADRDRLSIGFGGTWTAREG
                     QPLPDYDEAPVAAHLKGQDIAIAVDLGLGEGRATVWTCDLTHGYISINADYRS"
     gene            1161..1964
                     /locus_tag="VFV30_06975"
     CDS             1161..1964
                     /locus_tag="VFV30_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4847870.1"
                     /translation="MTEHLTGGVLAVIQAAADRAVMPRYQTLGADQIDAKAADDVVTV
                     ADREAELILAEGLSKLLPEAAIVGEEAVHADPALADRLGDALCWIIDPIDGTNNYAAG
                     KPPFGIMIALAEAGETVAGWIFDPLSGRLCHAHKGKGAFLGEDRIKARGSGVNPPIAA
                     ISLVFADPARREALKTHIAPHYTLVDIPRCAAEQYPRIVLGQNDITIFERTLAWDHAA
                     GVLFTNEAGGKVARPDGRAYRVDEHLRPSLIGASSPALWDELAARMAQL"
     gene            complement(1961..2281)
                     /gene="trxA"
                     /locus_tag="VFV30_06980"
     CDS             complement(1961..2281)
                     /gene="trxA"
                     /locus_tag="VFV30_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX4847871.1"
                     /translation="MATKAVTDASFAADVLGSDKPVLVDFWADWCGPCKMIAPALEEI
                     SEELADQVTIAKVDIMENTDISAKYGVQSIPLMVLFKNGEPVAQKLGAAPKSQLKGWL
                     ESVL"
     gene            complement(2349..>3046)
                     /locus_tag="VFV30_06985"
     CDS             complement(2349..>3046)
                     /locus_tag="VFV30_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX4847872.1"
                     /translation="SSKLPDRPAGPAALELPLPPPWLSRAAPDEPRPPRPLAPSSLGE
                     EQEADPPWPPGSGSGAARRGVLMHRLLERLPEVPAGERQAAAQAWLARMAGAFDAPAR
                     AEMVASALAVLEQKGWADLFGPGALAEVPIAATVGGQVIAGTIDRLLIGETTIRVVDF
                     KTARRPPASLDQVPVAILRQMAAYAAALEVTYPGRKVEAALLYTQVPRLIAIPDDLLA
                     VHKQALTRAQESF"
BASE COUNT          511 a         1013 c         1018 g          504 t
ORIGIN      
        1 acgccgcgtg ttgcccgggc cggctacaag gactgggggc gctgcgacct gacctatgtc
       61 gaactggccg agggaaccgc ggttgccggg gtgttcaccc gcaacgtgtg ctgttcgtcc
      121 gaagtcgagc tcggtcgcgc caacatcgcc cagggccagg cccgcgccct ggtggtcaat
      181 gccggcaatt cgaatgcctt caccggctat cgcgggcgcg aggcggttga ggcaatcatg
      241 gcccaggtgg ccacacacct tggctgcccg gccgaacagg tgttcgtctc ctcaaccgga
      301 gtgatcgggg ttcctttgcc aaaagacaag gcgcgcgccg ggatcgaagc cgcgctggtg
      361 gctgagccat gcggctggga agacgtggcc aacacgatcg gcaccaccga tacctttgcc
      421 aagggcgcga tggcccaggc cgtggtcgat ggccgcaccg tcacgctcgc cgccgtgatc
      481 aagggcagcg gcatgatcgc gcccgacatg gcaaccatgc tgggctatat ctttaccgac
      541 tgcgcgattg ccccgggcta tctccaggaa ctgcttggcg ccgccaacca gcgcaccttc
      601 agctgcatca ccgtcgatag cgatacctcg accagcgata ccgtgctggc ctttgccacc
      661 ggcatggccg ggaaccgcct gatcaccagc gcggattgtc caggcgctga tgcctttgcc
      721 gccgcgctgg aggatgtgtg ccgccaactg gcccacctgg tggtccgcga cggcgaaggt
      781 gcgcagaagt tcatcgcgat cagcgtgacc ggcgcggcaa gcgatgccag cgcccgcaag
      841 gtggggctgg ccatcgccaa ttcgccgctg gttaagaccg caatcgccgg cgaggatgcg
      901 aactggggcc gtgtggtcat ggccgtgggc aaggcgggcg agccggctga ccgcgatcgc
      961 ctgtcgatcg gctttggcgg cacctggacc gctcgcgaag gccagcctct gcccgactat
     1021 gacgaggccc cggttgccgc tcacctcaag ggacaggaca tcgcgattgc ggtcgatctg
     1081 ggcttgggcg aaggccgcgc cacggtgtgg acctgcgacc tgacccacgg ctacatctcg
     1141 atcaacgcgg attaccgctc gtgaccgagc atctgaccgg cggcgtgctg gcagtgatcc
     1201 aggccgcggc cgatcgagca gtgatgcccc gctaccagac gcttggcgcc gatcagatcg
     1261 acgccaaggc ggccgacgac gtggtgaccg tggctgaccg cgaagccgag ctgatcctgg
     1321 cagaagggtt gtcgaagctc ctgcccgaag cggcaatcgt cggcgaggaa gcggtgcacg
     1381 ccgatcctgc actggccgac cggctgggtg atgccctgtg ctggatcatc gatccgatcg
     1441 acgggaccaa caactatgcc gccggcaagc cgccgttcgg gatcatgatt gccctggccg
     1501 aagcgggcga gaccgtggcc ggctggatct tcgatccgct gtcgggccgg ctatgccatg
     1561 cccacaaggg caagggcgcc tttttgggcg aggaccggat caaggcccgc ggcagcgggg
     1621 tcaatccgcc gattgcggcg atttcgctgg tgtttgccga cccggcccgg cgcgaggcgc
     1681 tcaagaccca tatcgccccg cactacaccc tggtcgacat tccgcgctgt gcggccgagc
     1741 agtacccgcg catcgtgctg gggcagaacg acatcacgat cttcgaacgg acgctggcct
     1801 gggatcacgc cgccggcgtg ctgttcacca acgaggccgg cggcaaggtc gcgcggcccg
     1861 acggacgagc gtaccgggtc gacgagcacc tgcgcccaag cctgatcggc gcgtccagcc
     1921 cggccctgtg ggacgaactg gccgcgcgga tggcccagct ttaaagcacg ctttccagcc
     1981 agcccttgag ctggctcttg ggcgcagcac caagcttctg ggcaaccggt tcgccgttct
     2041 tgaacagcac catcagcggg atcgactgga cgccgtactt ggccgagata tcggtgtttt
     2101 ccatgatgtc gaccttggcg atggtcacct gatcggcaag ttcctcgctg atttcctcaa
     2161 gcgccggggc gatcatcttg cacgggccgc accagtcggc ccagaaatca accagaacgg
     2221 gcttgtcgct gcccagcacg tcggcggcga agctggcatc ggttacggct ttggtggcca
     2281 tcggttatct ccttggatgt cttgcctgcc aatgtagggc gcgctgcctg catggcaatc
     2341 gctgccggtc aaaaggattc ctgcgcccgg gtcaatgcct gcttgtggac agccagcaaa
     2401 tcatccggaa tcgcgatcag ccgcggaacc tgggtgtaaa gcagtgcggc ctcgaccttg
     2461 cgccccggat aggtcacttc gagcgcggcg gcataggccg ccatctgccg caggattgcc
     2521 accggcactt gatcgagact ggctggcgga cgccgcgcgg tcttgaaatc gaccacgcgg
     2581 atcgtggtct cgccgatcag cagccggtcg atcgtgccgg cgatgacctg cccgccaacc
     2641 gtggcggcaa tcggcacttc ggcaagggcg cctggaccaa acaggtctgc ccatcctttt
     2701 tgttcgagca cggcaagcgc cgacgccacc atctctgcgc gcgccggtgc gtcgaacgcg
     2761 ccggccatgc gcgccagcca ggcttgcgcg gcagcttggc gctcaccggc gggcacttca
     2821 ggcagccgtt cgagcaatcg gtgcatcaga accccgcgcc gtgccgcgcc agagcccgaa
     2881 cctggcggcc agggcggatc ggcttcctgc tcttcaccca gcgaagatgg tgcaagcggc
     2941 cgcggcggac gcggttcgtc gggagcagcc cggcttagcc agggtggcgg caagggcagc
     3001 tccagtgccg cagggccagc tggccgatcg ggcagcttgc tcgatc
//
LOCUS       DASUDV010000320         4382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_297291,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000320 DASUDV010000000
VERSION     DASUDV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297291"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFV30_06990"
     CDS             complement(<1..977)
                     /locus_tag="VFV30_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847873.1"
                     /translation="MKPIRFAFGLGLSLAAVPALAQQAPVSATSPDGAVRVSLSIDGD
                     GRAAYAVTRKGKQILAPSRLGFLFTDVVKIDRRLTVTGTEQRDFDETWTQPWGEWAEI
                     RNRYREFKVHLKETTALARAFTVTFRLYEDGVGFRYEFPEQPSMARTRIAEELTEFAF
                     AQDGTAWWKPAFLWNREEYLYNKTPLSSVSNAATPITIKLADGTHVALHEAALVDYSG
                     MAVARTEGTTLRASLTPGAGEPKVQKTGAWTTPWRTLIIADDAPGIYHSHLMLNLNEP
                     NKLGDVSWFKPGKFAGVWWNMIKGEWSWARGPKHGATTENVKRYIDFAAA"
     gene            complement(984..2615)
                     /locus_tag="VFV30_06995"
     CDS             complement(984..2615)
                     /locus_tag="VFV30_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX4847874.1"
                     /translation="MNSHNRIQPETCTDAATTPWWRGAAIYQVYPRSFQDSNGDGVGD
                     LPGITSRLEYIARLGVDAIWISPFFTSPMRDFGYDVADYCDVDPIFGTLADFDALVAR
                     AHDLGLKVLIDQVYAHTSDEHAWFAESRSSRDNPKADWYVWADPKADGSPPSNWQSVF
                     GGPAWTWDARRGQYYLHNFLSSQPQLNCHLPAVQDALLGVARFWLDRGVDGFRLDALN
                     FAMHDPQLRDNPPAPVNGKPRTRPFDFQQRIYNQSHADIPLFLERIREVLDQYDAIFT
                     VAEVGGEGALGEMHAFTQGENRLNSAYGFNFLYADRLTPRLVAGALGEWPEVAGTGWP
                     SWALENHDAPRALSRWCRPEHRDAFARLKVMLLASLRGNIIIYQGEELGLTQVEIAFG
                     QLMDPEAIANWPLTLSRDGARTPMPWTAQDEQGGFTQGQPWLPLGDENMARAVDRQEG
                     DETSLLALTRELVALRRGNDVLRSGTCEVLVADDTRLVLRRTLNEQAVLAVFNFSPEP
                     ADWPAEAGIDGEIIKSVNGAEPGHLPPFGAIIIGR"
     gene            complement(2637..4364)
                     /locus_tag="VFV30_07000"
     CDS             complement(2637..4364)
                     /locus_tag="VFV30_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX4847875.1"
                     /translation="MRARPPEQEVVYFVLPDRFENGDPKNDRGGIKGDRLRHGFDPTA
                     RGFYHGGDLKGLTSRLDYIQALGASAIWFAPIFRNKPVQGPKGDESAGYHGYWVTDFT
                     TVDPHFGTEADFKAFVDAAHARGMKVYMDILVNHTADVIQYRNPGDYSYRSKADYPFS
                     RRGGVQGAAINAGFAGDHLADPANWARLTDPSFAYEPFVPKGEEQAKTPAWLNDPIYY
                     HNRGNSNWVGEGSIYGDFVGLDDLATEHPRVVAGMIEIYASWIDRFGIDGFRIDTARH
                     VNPEFWQAFVPATLERARARGIPNFHIFGEVATGEVDPALTARWTRIAGLPSNLDMAF
                     AAAAQRVVSGKAGTDMLARLIEDDALYEGGELAANRLPTFLGNHDFGRFSMFVKQANP
                     AIGDAELLARVKLGHAMLLTLRGVPTIYYGDEQGFVSDGNDQKAREDMFASQVAEYND
                     NDLIGTDATTAQANFDQNHPLYRQIAELAALRRSTPALTSGKTRIRAFSDKPGLFAVS
                     RTDPQTGAEVVLAFNTSATALSARIAVDPLSGSFVTLVGQCSPKADAPGSLSINLPAF
                     GYAVCAARN"
BASE COUNT          780 a         1371 c         1444 g          787 t
ORIGIN      
        1 gcggcagcga aatcgatgta gcgcttgacg ttctcggtcg tcgcgccgtg cttcggcccg
       61 cgcgcccacg accattcgcc cttgatcatg ttccaccaca cgccggcgaa cttgccgggc
      121 ttgaaccagc tgacatcacc cagcttgttg ggttcgttga ggttgagcat caggtggctg
      181 tggtaaatgc ccggcgcatc atcggcgatg atcagggtgc gccagggtgt ggtccaggcg
      241 ccggtcttct gcaccttggg ctcacccgcg cccggggtga gcgaggcgcg cagggtcgtg
      301 ccttcggtgc gcgccacggc catgcccgaa tagtcgacca gcgcggcctc gtgcagggcc
      361 acatgggtac cgtcggccag cttgatggta atcggggtgg cggcgttgct gaccgaagac
      421 agcggggtct tgttgtagag gtattcctcg cggttccaca ggaaggccgg tttccaccag
      481 gccgtgccat cctgggcaaa ggcgaattcg gtcagctcct cggcaatcct ggtcctggcc
      541 atgctgggct gctcgggaaa ttcatagcgg aagcccacgc cgtcttcgta aagccggaag
      601 gtgacggtga aggcacgggc cagcgcagtg gtttccttga ggtggacctt gaattcgcga
      661 tagcggttgc ggatctcggc ccattcaccc cagggctggg tccaggtttc gtcgaagtcg
      721 cgctgttcgg tgccggtaac cgtcagcctg cggtcgatct tgacgacatc ggtgaacagg
      781 aagcccagcc ttgaaggcgc caggatctgc ttgcccttgc gggttaccgc ataggccgcg
      841 cgaccatcgc catcgatcga caggctgacc cgcaccgcgc catcgggcga agtggcgctg
      901 accggtgcct gctgggccag ggccggaacc gcagcaaggg aaaggccaag accaaaggcg
      961 aagcggatcg gcttcattgc agattatctc ccgatgatga tcgccccgaa gggcggcagg
     1021 tggccgggtt cagccccgtt cacgcttttg atgatttcgc cgtcgatgcc ggcttcggcg
     1081 ggccaatccg ccggctctgg cgagaagttg aacaccgcca gcacggcctg ttcgttcagt
     1141 gtgcgccgca ggaccagacg ggtgtcgtcg gccacaagca cttcgcaggt gccgctgcgc
     1201 aacacatcat tgccccggcg cagggcaaca agttcgcggg tcagcgccag cagcgaggtt
     1261 tcatcgcctt cctgccgatc aaccgccctg gccatgttct cgtcgccaag cggcagccag
     1321 ggctggccct gggtaaatcc gccctgctcg tcctgcgcgg tccagggcat tggggtgcgc
     1381 gcgccatcgc gcgaaagggt cagcggccag ttggcgatcg cttcgggatc catcagctgg
     1441 ccaaaggcga tctcgacctg ggtcagcccc agttcctcgc cctgatagat gatgatgttg
     1501 ccgcgcagcg atgccagcag catcaccttg agccgggcaa aggcatcgcg gtgttcgggc
     1561 ctgcaccagc gcgaaagcgc acgcggcgcg tcgtggttct ccagcgccca gcttggccag
     1621 ccggtccccg caacttcggg ccattcgccc agcgccccgg ccaccaggcg cggggtcagg
     1681 cgatcggcgt agaggaaatt gaagccatag gcgctgttca gccggttttc gccctgggtg
     1741 aaggcgtgca tttcgccaag cgcgccctcc ccgccaacct cggccacggt gaagatcgca
     1801 tcgtactgat ccagcacttc gcgaatgcgt tcgaggaaca agggaatgtc ggcgtgcgac
     1861 tggttgtaga tccgctgctg gaaatcgaac ggccgggtgc gcggcttgcc attgaccggc
     1921 gccggcggat tgtcgcgcag ctggggatcg tgcatcgcga agttcagtgc gtcgagccgg
     1981 aacccgtcca ccccgcgatc cagccagaac cgcgcgaccc ccagcagcgc gtcctgaacc
     2041 gctggcaggt ggcagttgag ctgcggctgc gagctgagaa agttgtgcag gtaatactgc
     2101 ccgcgccgcg catcccaggt ccaggccgga ccgccgaaca ccgattgcca gttcgatggc
     2161 ggcgatccgt cggccttggg atcggcccag acataccaat cggccttcgg gttatcgcgg
     2221 cttgagcggc tttcggcaaa ccaggcgtgc tcgtcagagg tatgggcata gacctgatcg
     2281 atcagcacct tgaggcccag atcatgcgcc cgcgcaacca gcgcatcgaa atcggccagg
     2341 gtgccgaaga tcggatcgac atcgcaatag tcggccacgt cataaccgaa atcgcgcatc
     2401 ggcgaggtga agaacggcga aatccagatc gcgtccaccc ccagccgggc gatgtattca
     2461 agccgggaag ttatgccggg cagatcgccc accccgtcgc cattcgaatc ctggaaactg
     2521 cgcggataga cctgatagat cgccgcgccg cgccaccagg gcgtggtggc ggcatcggtg
     2581 caagtttcag gctggattcg attgtgactg ttcatcggtc ttgaattcgg acttgctcag
     2641 tttcgtgcgg cgcaaacggc atagccgaat gcgggaagat tgatggacag gctgcccggc
     2701 gcatcggcct tgggcgaaca ctgtccgacg agtgtgacaa acgatccgct gagcggatca
     2761 accgcgatcc gggccgaaag cgccgtagcg gaggtgttga aggccagcac cacttcagcc
     2821 ccggtttggg gatcggtgcg ggaaaccgcg aacaggcccg gcttgtcgga aaaggcgcgg
     2881 atccgggtct tgccgctggt caatgccggg gtgctgcggc gaagcgccgc aagctcggcg
     2941 atctggcggt agagcggatg gttctggtcg aaattagcct gggccgtggt ggcatcggtg
     3001 ccgatcaggt cgttgtcgtt gtattcggca acctgcgaag cgaacatgtc ctcgcgcgcc
     3061 ttctggtcgt tgccatcaga aacgaagccc tgctcgtcgc cgtaatagat cgtcggcacc
     3121 ccgcgcaggg tcagcagcat ggcatggccc agcttgaccc gcgcgagcag ttcggcatcg
     3181 ccgatcgcgg gattggcctg cttcacgaac atcgagaagc ggccaaaatc gtgattgccc
     3241 aggaacgtcg gcaggcggtt ggccgcaagc tcgccccctt cataaagcgc atcgtcttca
     3301 atcagccggg ccagcatgtc ggttcctgcc ttgccggaaa ccacccgctg ggcggcagcg
     3361 gcaaaggcca tgtcgagatt gctgggcagc ccggcaatcc gcgtccagcg cgcggtcagc
     3421 gcggggtcaa cctcgccggt ggcaacttcg ccgaagatgt ggaagttggg aatgccgcgg
     3481 gcccgtgcgc gttcgagcgt ggccggcacg aaggcctgcc agaattcagg gttcacgtgc
     3541 ctggccgtat cgatgcggaa gccgtcgatc ccgaaccggt cgatccagct ggcgtagatc
     3601 tcgatcatcc cggcgaccac ccgcgggtgt tcggtcgcca gatcgtcaag ccccacgaaa
     3661 tcgccataga tcgaaccctc gccaacccag ttcgaattgc cgcgattgtg gtaatagatc
     3721 ggatcgttga gccaggccgg ggtcttggcc tgttcttcgc ccttgggcac gaaaggctcg
     3781 taggcgaacg agggatcggt gagcctggcc cagttggccg gatcggccag gtgatcgccg
     3841 gcaaagcccg cgttgatcgc cgcgccttgc acgccgccgc ggcgcgaaaa gggatagtcg
     3901 gccttgctgc gataggaata gtcgccagga ttgcggtact ggatgacatc ggccgtgtga
     3961 ttgaccagga tgtccatgta gaccttcatg ccccgggcat gggcggcatc gacaaaggcc
     4021 ttgaaatccg cctcggtgcc gaaatgcgga tcaaccgtgg tgaaatcggt tacccagtag
     4081 ccgtgatagc ccgcgctttc atcgcccttg gggccctgca ccggcttgtt gcggaagatc
     4141 ggcgcgaacc agatcgcgct ggcacccagc gcctggatat agtccagccg gctggtcagc
     4201 cccttgagat cgccgccgtg gtaaaagccg cgcgcggtcg gatcgaaacc gtgccgcagc
     4261 cggtcgccct tgatcccgcc cctgtcgttc ttgggatcgc cgttctcgaa ccggtcgggc
     4321 aggacgaaat agacaacttc ctgctcgggc gggcgggccc gcagctctgc cgccggctgc
     4381 gc
//
LOCUS       DASUDV010000321         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_871885,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000321 DASUDV010000000
VERSION     DASUDV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871885"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFV30_07005"
     CDS             <1..437
                     /locus_tag="VFV30_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847876.1"
                     /translation="AFVRTDHATAFAVLFFGGMLIFPVSSLIGKIALGRSASAKGNPL
                     GLLALESTIAMIGCLIAAWLLLKDRPDWVFPVAAIAVGTHYLPFKTAYGDRTYWLLGA
                     AITAIGCAGIFLLPGQTLAVILAVAVTELVFGLILIRAGTRA"
     gene            complement(434..835)
                     /locus_tag="VFV30_07010"
     CDS             complement(434..835)
                     /locus_tag="VFV30_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847877.1"
                     /translation="MPAPRLASAALAAAALAFALPSAVQAKANGNGRGQGAHAGHGPA
                     AKKPPKAARAKPCPPGLAKKNTGCLPPGQWRKGDRLPEAWAVQFIAYAALPDFYRSRY
                     PAQDGRRYLYRDGRVFVIDAVTRTVLDVILR"
     gene            complement(913..1518)
                     /locus_tag="VFV30_07015"
     CDS             complement(913..1518)
                     /locus_tag="VFV30_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX4847878.1"
                     /translation="MRVSIFPLPGAILFPGMQLPLHIFEPRYRALVKDALARDRRIGM
                     IQPQRAEEGAPLYRIGCLGKIEEVEALDDGRFNLILEGEARFRLVRELDAATPFRQIE
                     AELLDEEDFPALSPVERAGFEREARRFSDSQGYSVDWEAVTRLDDESLINGTSQIVPF
                     DIASKQALLEAPSLAARCELLVQLMQFFGRRDSDDPKVTLQ"
     gene            complement(1536..>2357)
                     /locus_tag="VFV30_07020"
     CDS             complement(1536..>2357)
                     /locus_tag="VFV30_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4847879.1"
                     /translation="SLVILDFWAEWCGPCKALTPVLEKVAADYADKGVVLAKVNVDEE
                     GFIASQFQVRSIPTVYAIFQGQPVADLTQARSESQLKGILDQLLAKLPVKAGGAPAQD
                     VAPLLAMGEEVLSGGEADRAAAIFSQIIEIDPGSAAAYGGLIRAVTAMGETAQADEML
                     AALPPELAGDAEIARARTALELAKNRPEDSELAALRAAATADNPAGQLAYAEAAFAAG
                     DRDGAADTLLAMIAADRAWNDGAAKAKLLQIFEAVGLEDAWVAAQRRRLSLILFG"
BASE COUNT          340 a          830 c          814 g          373 t
ORIGIN      
        1 cggccttcgt ccgcaccgac catgccaccg ccttcgccgt gctgttcttc ggcgggatgc
       61 tgatcttccc agtctccagc ctgatcggaa agatcgcttt ggggcgcagc gcgtcggcca
      121 agggcaaccc gctcgggctg ctcgcgctgg aaagcaccat cgcgatgatc ggctgcctga
      181 tcgcggcctg gctgctgctg aaggaccggc ccgattgggt cttcccggtc gccgcgatcg
      241 cggtgggcac ccattacctg ccgttcaaga ccgcctatgg cgaccggacc tattggctgc
      301 tcggcgcggc catcactgcg atcggctgtg ccgggatctt cctcctgccg ggccagaccc
      361 tggcggtgat cctggccgtg gcggtgaccg agctggtctt cggcctgatc ctgatccgcg
      421 cgggcacccg cgcctagcgc aggatcacgt ccagcaccgt ccgcgtcacc gcgtcgatca
      481 cgaagacccg tccgtcacgg taaagatagc ggcgcccgtc ctgggccgga tagcggctgc
      541 ggtagaaatc gggcagcgcg gcataggcga tgaactgcac cgcccaggcc tcgggcagcc
      601 ggtcgccctt gcgccactgc cccggcggca ggcagccggt gttcttcttg gcgaggcccg
      661 gcggacaggg cttggcgcgg gcggctttgg gcggcttttt ggcagcgggg ccatgcccgg
      721 cgtgcgcgcc ctggccgcgg ccgttgccgt ttgccttcgc ctgcaccgcg ctcggcaagg
      781 caaaggccag ggcggcagcc gcaagggcgg cggatgcaag gcggggtgcg ggcatggcgg
      841 ttctccagtg cggtgaatca gggccgcatc ctagcgcgcc ctggccgcaa gcggaccccg
      901 taaaatgcgg ccctactgca atgtcacttt cggatcgtcg ctgtcgcgcc ggccaaagaa
      961 ctgcatcagc tgaaccagaa gctcgcaccg cgcggccagc gagggtgcct cgagcagcgc
     1021 ctgcttcgag gcgatgtcga acggcacgat ctggctggtg ccgttgatca gcgattcgtc
     1081 gtcgagccgg gtcacggcct cccaatcgac cgaatagccc tggctgtcgg aaaagcggcg
     1141 tgcctcgcgc tcgaacccgg cgcgctcgac cggggagagc gcggggaagt cctcctcgtc
     1201 gagcagctcg gcctcgattt ggcggaacgg ggtggcggca tccagttcgc ggaccagacg
     1261 gaaccgcgct tcgccctcca ggatcaggtt gaaccggccg tcgtcgagcg cctcgacctc
     1321 ttcgatcttg cccaggcagc cgatccggta gagcggcgcg ccctcctcgg cccgctgcgg
     1381 ctggatcatc ccgatccgcc ggtcgcgcgc cagcgcatcc ttgaccagcg cgcggtaacg
     1441 cggctcgaag atgtgcagcg gcagctgcat tcccggaaac aggatcgcgc cgggcagggg
     1501 aaagatcgaa acgcgcatcg cgggcggtcc cgcgcctagc cgaacaggat cagcgagagc
     1561 cggcggcgct gggcggccac ccaggcgtcc tccagcccca ccgcttcgaa gatctgcagc
     1621 agcttggcct tggccgcgcc gtcgttccag gcgcggtcgg cggcgatcat cgccagcagg
     1681 gtatcggccg cgccgtcgcg gtcaccggcg gcgaaggcgg cttcggcata ggccagctgc
     1741 ccggccgggt tgtcggccgt ggcggcggcg cgcagcgcgg ccagctcgct gtcctcgggc
     1801 cggttcttgg ccagctccag cgcggtgcgg gcgcgggcga tctcggcatc gccggccaat
     1861 tccggcggca gcgcggccag catctcgtcg gcctgggcgg tttcgcccat cgcggtcacg
     1921 gcgcggatca gcccgccata ggccgcggcg ctgcccggat cgatttcgat gatctgcgaa
     1981 aagatcgccg ccgcccggtc cgcctctccg ccggacagca cctcttcgcc catcgccagc
     2041 agcggagcga catcctgcgc cggggccccg ccggccttga ccggaagctt ggccagcagc
     2101 tggtcgagaa tgcccttcag ctgcgattcg ctgcgggcct gggtcaggtc ggccaccggc
     2161 tggccctgaa agatcgcata gaccgtgggg atcgagcgga cctggaactg gctggcgatg
     2221 aagccttcct cgtcgacgtt gaccttggcc aggaccacgc ccttgtcggc atagtcggcc
     2281 gcgacctttt ccagcaccgg ggtcagcgcc ttgcacggac cgcaccattc ggcccagaaa
     2341 tccaggatca cgagcga
//
LOCUS       DASUDV010000322         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1136817,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000322 DASUDV010000000
VERSION     DASUDV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136817"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /gene="rplT"
                     /locus_tag="VFV30_07025"
     CDS             <1..327
                     /gene="rplT"
                     /locus_tag="VFV30_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX4847880.1"
                     /translation="AKHKRILDQAKGYRGRRKNTIRVARQAVEKAGQYAYRDRKVKKR
                     TFRALWIQRINAAVRAEGLTYSQFIHGAKLAGIELDRKAMADLAMNEGGVFTAVIAQA
                     KAALPA"
     gene            514..1404
                     /locus_tag="VFV30_07030"
     CDS             514..1404
                     /locus_tag="VFV30_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847881.1"
                     /translation="MLKQPLTDAVRTRFFMPSPALAPYISTYYLTEFAVKPDEVVADW
                     LHPEWANIRMSDTGSWQSAVGGQGLATLPRMIGTGPTALATHFAVTGPARTWGIGILP
                     RGWLRLVDAPASQLADTTETVCPGTRFAAFAPLYDQVFAGAPDPAGEAARIDAFLLDL
                     LERRPAHEDDTRVRAAHAALVDDGVASVGELAERLGISPRSLERLSLRAFGFSPKLLL
                     RRQRFLRSLAQFLLDPSLTWIRTLDCHYVDQAHFVRDFRRFMTMSPSAYAALDHPVLR
                     SAARARAVAAGAAVQALHKP"
     gene            1490..2416
                     /locus_tag="VFV30_07035"
     CDS             1490..2416
                     /locus_tag="VFV30_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4847882.1"
                     /translation="MSPSHSVTVRFFPPPEELRPYFTTFYLTEIDPGQEAALHDSLHP
                     EWAGLRFFTPRGPLAWTDAGERLDGARFQATGPTSHPIHFALPKTRMWGIGLLPLGWA
                     RFVNQPAAEFANRVLDGQTDPAFATFRPLADALLATGGDVDAELDAITAFFQGFSAEP
                     FADERRLLAIHVALVDPEVVTVADLVQRVAASQRTVERLCDRHFGFSPKLLLRRQRFM
                     RSLAQFMLDPSLKWIGAMDPNYHDQAQFVRDFREFMGLSPREYAAQPHPVLERFLHER
                     MKAHGAPVQTLDKPIPGRARNPARPAPAKLGF"
     gene            2484..>2708
                     /locus_tag="VFV30_07040"
     CDS             2484..>2708
                     /locus_tag="VFV30_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX4847883.1"
                     /translation="MDHAATREQALARIAAAQDLDALEKLRVEFLGKQGSISGLLKTL
                     GAMSPEDRQVAGPQIHALRESVTEALAEAKA"
BASE COUNT          405 a          991 c          866 g          446 t
ORIGIN      
        1 gccaagcaca agcggattct cgaccaggcc aagggctatc gcggtcgccg caagaacacc
       61 atccgcgtcg cgcgccaggc cgtcgaaaag gccggccagt acgcttaccg cgaccgcaag
      121 gttaagaagc ggaccttccg cgccctgtgg atccagcgca tcaacgccgc tgtccgcgcc
      181 gaaggcctga cctattcgca gttcatccac ggcgccaagc tggccggcat cgagctggac
      241 cgcaaggcca tggccgatct ggcgatgaac gaaggcggtg tcttcaccgc tgtcatcgcc
      301 caggcgaagg ccgccctgcc ggcctgaacg ccgcaggtgt gaaaaccgat cgggcgcggg
      361 atgcggcaca ggccgcctcc cgcgcctttt cgtttgcgct ccccccaatc gggtattgtc
      421 cgccgcccgg cgattccaca ccatgatgcc ggtcccggtg tttgcgctgt cccgggatca
      481 gggggcgcgt gtgaccatgc cgtttgaacg ccaatgctga agcagccgct gaccgatgcg
      541 gtccggactc ggttcttcat gccatcgccg gcgctggcgc cgtacatttc cacctattac
      601 ctgaccgaat tcgcggtgaa gcccgacgag gtggtggccg actggctgca ccccgaatgg
      661 gccaacatcc ggatgagcga taccggcagc tggcaatcgg ccgtcggcgg ccagggcctg
      721 gccacgctgc cgcggatgat cggcaccgga ccaaccgcgc tggccaccca tttcgcggtc
      781 accggcccgg cccgcacctg gggcatcggc atcctgccgc gcggctggct gcggctggtt
      841 gacgccccgg caagccagct ggccgacacc accgagacgg tctgccccgg cacccgcttt
      901 gccgcctttg ccccgctcta tgaccaggtc tttgccggcg cgcccgatcc cgcgggcgaa
      961 gccgcccgga tcgacgcctt cctgctggat ctgctggagc gccgcccggc gcacgaggac
     1021 gacacccggg tccgcgccgc ccatgccgcg ctggttgacg acggggtcgc ctcggtgggc
     1081 gaactggccg agcggctggg catttcgccg cgctcgctcg agcggctctc gctcagggcc
     1141 ttcggcttct cgcccaagtt gctgctgcgc cgccagcgct tcctgcgcag cctggcccag
     1201 ttcctgctcg atccctcgct cacctggatc cgcacgctgg attgccacta tgtcgaccag
     1261 gcccatttcg tgcgcgattt ccggcggttc atgaccatga gccccagcgc ctatgccgcg
     1321 ctggatcacc cggtgctgcg ctcggccgcg cgggcccggg ccgtggcggc gggcgcggca
     1381 gtccaggcgc tccacaaacc ttgagcccgt cccggccctg aacccgacct tgtctttgcc
     1441 gctgccttgc tgcaagctgc cggcacaggg tcgaagcaag gagtcttcgg tgtcgccctc
     1501 tcacagtgtc acggtgcggt tcttcccccc gccggaagaa ttgcgcccct attttacaac
     1561 cttttacctg accgagatcg atcccggcca ggaagcggcc ctgcacgaca gcctgcatcc
     1621 cgaatgggcg gggctgcggt tcttcacccc gcgcgggccg ctggcctgga ccgacgccgg
     1681 cgaacggctg gacggcgcgc ggttccaggc caccggcccg acatcccatc ccatccactt
     1741 tgcgctgccc aagacgcgga tgtggggcat cggcctcttg ccgctgggct gggcccgctt
     1801 cgtcaaccag cccgccgccg aattcgccaa ccgcgtgctc gacggccaga ccgatccggc
     1861 ctttgccacg ttccggccgc tggccgatgc cctgctggca accggcggcg acgtggatgc
     1921 cgagctggac gcgatcacag ccttcttcca gggcttttcg gccgaaccct ttgccgatga
     1981 gcgccgcctg ctggcgatcc acgttgccct ggtcgatccc gaggtggtga ccgtggccga
     2041 tctggtccag cgggttgccg ccagccagcg cacggtggag cggctgtgcg accggcattt
     2101 cggcttttcg cccaagctgc tgctgcgccg ccagcgcttc atgcgcagcc tggcccagtt
     2161 catgctcgat ccctcgctca aatggatcgg ggcgatggac cccaattacc acgatcaggc
     2221 ccagttcgtg cgcgacttcc gcgaattcat gggcctgtcc ccgcgcgagt acgccgccca
     2281 gccccacccg gtgctggaac ggttcctgca cgaacggatg aaggcccacg gcgcgccggt
     2341 gcagaccctg gacaagccga tccccggacg ggcgcgcaat ccggcgcggc ccgccccggc
     2401 gaaattaggg ttttgacccc gcgcgcggct tggctaaagg cgcgccttca acaactctag
     2461 ccaggtgatt ggaagcaaag cccgtggacc atgccgcaac ccgtgaacag gcgctggcac
     2521 gtattgccgc cgcgcaggat ctcgacgcgc tggaaaagct gcgggttgag tttctgggca
     2581 agcagggttc gatctcgggc ctgctgaaga ccctgggcgc gatgtcgccc gaggaccgcc
     2641 aggtggccgg gccgcagatc cacgccctgc gcgaaagcgt gaccgaggcg ctggccgagg
     2701 ccaaggcc
//
LOCUS       DASUDV010000323         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1822123,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000323 DASUDV010000000
VERSION     DASUDV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822123"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFV30_07045"
     CDS             complement(<1..607)
                     /locus_tag="VFV30_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4847884.1"
                     /translation="MARYTFYTNPMSRGQIARWALHEVGADYEQVLVDWAAKPAALLA
                     ANPMGKVPTVIHHAPGGDRVVTECAAICAYLGDAHPDAGLAPTDAERADFYRWMFFAA
                     GPVEQAIVGKSMGWDVPEGREGMVGFGSFERTLGALEGHFAANAFVCGDRFTMADVYV
                     GSQVDWGLTFGSIPPREAFVAYAERLQARHAYKEAKGIDHAL"
     gene            complement(661..1173)
                     /locus_tag="VFV30_07050"
     CDS             complement(661..1173)
                     /locus_tag="VFV30_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847885.1"
                     /translation="MSPDTILRCNCGKVECRATGAPFYTAVCYCDHCQEAARLIGESG
                     NGPAVMEADGGTALCLIRDDRFQITKGANLLTPHLRDPASATSRMVASCCHSAMFLCF
                     SDGRFWKSAMVGRIVGARPEIEARLCTRFRESALPWPDNAPRYSGFPARALFKIARQW
                     LSMRLGKKAV"
     gene            complement(1170..1955)
                     /locus_tag="VFV30_07055"
     CDS             complement(1170..1955)
                     /locus_tag="VFV30_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4847886.1"
                     /translation="MATLQGSWVWYELITPDPAGAKEFYDAVVGWTMQPGTPESGGYG
                     FIANADGGLTGGILELSADMADHGANPCWLGYVGVDDVDASCAAIAAAGGAVLMPARD
                     VPMAGRIAMVADCCGAPFYVMTPTPPPGGGESTCFSALPNPGRCGWNELYCADLSAAE
                     RFYTGLFGWTLPDAMDMGPMGKYQFIAHDGVTVGAMMQKPEAVPVSVWGHYFWVDSIA
                     AAKSLVAAKGGQVVNGPHQVPGDLWIIQGIDPQGALFSLVGGE"
     gene            complement(1969..2325)
                     /locus_tag="VFV30_07060"
     CDS             complement(1969..2325)
                     /locus_tag="VFV30_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX4847887.1"
                     /translation="MSYVEGFLIPVPADKEDAYRKQAEAAAPIFLDLGATRVVENWEA
                     DVPDGTSTDFRKAVKAEPGEKIVFSWIVYPDKAARDAAHAAMMEDPRFEALGEMPFDG
                     KRMIFGGFSTFLDQGK"
     gene            complement(2322..>2646)
                     /locus_tag="VFV30_07065"
     CDS             complement(2322..>2646)
                     /locus_tag="VFV30_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX4847888.1"
                     /translation="ACMVLSDTIHVILLTHGKWQGFTTRPICPPGSSEVSLALTCASR
                     EAVDSMVAAGAAAGGTADINPPEDHGFMYQRSLLDPDGHVWEPFWMDPAFAGGEATKE
                     EEDAA"
BASE COUNT          476 a          883 c          844 g          443 t
ORIGIN      
        1 tcagcgcgtg gtcgattccc ttggcctcct tgtaggcgtg ccgggcctgc agccgctcgg
       61 cataggcgac aaaggcctcg cgcggaggga tcgagccgaa ggtcaggccc cagtcgacct
      121 ggctgccgac atagacgtcg gccatggtga agcggtcccc gcagacaaag gcgttggcgg
      181 caaaatggcc ctccagcgcg ccaagcgtgc gctcaaaact gccgaagccg accatgccct
      241 cgcgcccctc gggcacgtcc cagcccatcg acttgccgac gatcgcctgc tcgaccgggc
      301 cggccgcgaa gaacatccag cggtagaagt cggcccgctc ggcgtcggtt ggggcaaggc
      361 ccgcatcggg atgggcatcg cccagatagg cgcagatcgc ggcgcattcg gtcaccaccc
      421 ggtccccgcc gggggcgtgg tggatcacgg tcggcacctt gcccatcggg ttggcggcca
      481 gcagcgcagc gggcttggcg gcccaatcaa ccagcacctg ctcataatcc gcgccaacct
      541 cgtgcagcgc ccagcgggca atctggccgc ggctcatcgg gttggtgtag aatgtatagc
      601 gcgccatggc aggtcatctc ctttgtgaat gtggagaaca taaaaagaac aaacaaaagg
      661 tcaaaccgct ttcttgccca gccgcatgct cagccactgc cgcgcgatct tgaacagggc
      721 acgggcggga aatccgctgt aacgcggggc attatcgggc cagggcaagg cgctttcgcg
      781 aaatcgcgtg cagagccgcg cctcgatttc cggtcttgcg ccgacgatcc tgccgaccat
      841 cgccgatttc cagaaccgcc cgtcagaaaa gcacaggaac atggccgaat ggcagcagct
      901 ggcgaccatg cggctggtgg cggacgcggg atcgcgaagg tgcggggtga ggaggtttgc
      961 acccttggtg atctgaaagc gatcgtcccg gatcaggcac agcgcggtgc cgccgtcggc
     1021 ttccatcacg gcggggccat tgccgctttc cccgatcaac cgcgccgctt cctggcagtg
     1081 atcgcaatag cagaccgcag tatagaacgg agcgccggtt gcccggcatt ccaccttgcc
     1141 gcagttgcag cgcaggatgg tatccgggct cactctccgc cgaccaggct gaacagcgcg
     1201 ccttgcggat cgatcccctg aatgatccac aaatcgcccg gaacctgatg cggaccattg
     1261 accacctggc cgcccttcgc cgcgacgagc gatttggcgg cagcgatgct gtccacccag
     1321 aagtaatgac cccagaccga gaccggcacg gcttcgggct tctgcatcat cgctccaacc
     1381 gtgacaccgt cgtgggcaat gaactggtat ttgcccatcg gacccatgtc catggcatcg
     1441 ggcagggtcc agccgaacag gccggtgtag aaacgttctg ccgcggagag gtctgcgcag
     1501 tacaactcgt tccagccgca ccggccgggg ttgggcagag ccgagaagca ggtgctttcc
     1561 ccgccgccgg ggggcggggt gggggtcatc acatagaacg gcgcgccgca acaatcggcc
     1621 accatcgcga tccgcccggc catcggcaca tcgcgggccg gcatcagcac cgcgccgccc
     1681 gccgccgcaa tcgcggcgca gctggcatcg acatcgtcga ccccgacata gccgagccag
     1741 cacggattgg cgccgtgatc ggccatgtct gcggaaagct ccagaatgcc gccggtcagc
     1801 ccgccgtcgg cattggcgat gaagccatag ccgccgctct caggggtgcc gggctgcatg
     1861 gtccagccca ccaccgcgtc atagaattcc ttggctccgg ccggatcggg ggtgatcagt
     1921 tcataccaca cccagctgcc ttgcagtgtt gccatagcgc ctgcgctcct atttgccctg
     1981 atcaagaaat gtcgagaagc cgccgaagat catgcgtttc ccgtcaaagg gcatttcgcc
     2041 cagggcttcg aagcggggat cttccatcat cgccgcgtga gcggcgtcgc gggccgcctt
     2101 gtccggatag acgatccagc tgaagacgat cttctcgccc ggttcggctt tcaccgcctt
     2161 gcggaagtcg gtcgaggtgc catccggcac atccgcttcc cagttttcaa ccacgcgggt
     2221 ggcgccaagg tcgaggaaga tcggcgccgc cgcttcggct tgcttgcgat aggcgtcctc
     2281 cttgtcggca gggacaggga tcagaaatcc ttcgacatag ctcatgctgc gtcctcctcc
     2341 tccttggtgg cctcgccgcc ggcaaaggcc gggtccatcc agaacggttc ccagacgtgg
     2401 ccatcgggat cgagcagcga gcgctggtac atgaagccgt gatcctcggg cgggttgatg
     2461 tcggccgtgc cgccggctgc ggctccggcg gccaccatgc tgtcaaccgc ctcgcggctg
     2521 gcgcaggtca gggccagcga cacttcgctt gatcccggcg ggcagatcgg ccgggtggta
     2581 aagccctgcc acttcccgtg ggtcagcagt atcacgtgga tggtgtcgga caggaccatg
     2641 caggcc
//
LOCUS       DASUDV010000324         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2065004,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000324 DASUDV010000000
VERSION     DASUDV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065004"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFV30_07070"
     CDS             complement(<1..374)
                     /locus_tag="VFV30_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847889.1"
                     /translation="MIAAAGYAGLSIALAFDRTAASDPGAAARIPEPFRQEALSSQGQ
                     ALLLAGQAGQIAPLAEALVRRDPLGPQAAGLLGTARLAQGDARGAETAFRLSAKLGWR
                     DAATQVYWLQAALAAGDLTRAGT"
     gene            complement(427..2637)
                     /locus_tag="VFV30_07075"
     CDS             complement(427..2637)
                     /locus_tag="VFV30_07075"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX4847890.1"
                     /translation="MNDHPILRDIQPTNSVDLHDEPEVFQAMTRGTFDLRYIAAAIRS
                     NLLLISAIILVALGAALVITLLDTPRYTANTTIQINDSSDRVIGENDDQTAQESNLYD
                     VDRFLKTQTDILRSRGLAQRVAQKLKLQTRPEFYAAMESKAPGAGVTAEAMRNQVVGL
                     LLGNLKVNLPRDSRIVTISFESADPSMSAEIANAFATEFIQSNLQRKFDSSSYAREFV
                     ANQMAEAKQRVEQAERNLNDYSRSTGLIRTGDAAGETGEATTGGSVTAASLLQLNQAA
                     NQATAERVAAEGRWRAISSVPLLSSREVLGNATVSGLMTDRATTQAELQRELSKHLED
                     YPTVREKRAQLARLDAELQSAAQSVRNSIRAEMLAAQGTEQALLAKLENLKGETLREQ
                     DKTVQYGLLKREADTNRTLYDGLLKRYNELNATAGIASSNVSIIDSAERPTIPTSPNM
                     LKNLLLALLVGVGLAALTLFIKDQLDDAIRVPEDVESKLRLPLLGVVPKATGRDPDSE
                     MGDPKSPVSEAYNSLRAALLYSTSAGLPQVMLVTSAQSAEGKTTTSFATAASLARMGR
                     KVALIDADLRRPSIHRRFGLPNDRGLTDLLTSGDPVESAILATPQENLFVIASGPIPP
                     SPTELVSSPRMEHVVQELARTYDVVVIDSPPILGLADAPALSALADGVIFVVESDRSR
                     RGSLKTALRRLRAMRPILLGAVLTKFDPTRSGNRYSEYYGYQYYTYEQSDRERD"
BASE COUNT          444 a          858 c          920 g          498 t
ORIGIN      
        1 gtgccggccc gggtcaggtc accggccgcc aatgcggcct gcaaccaata gacctgggtc
       61 gcggcatcgc gccaacccag cttggctgac aggcgaaagg cggtttcggc cccgcgtgca
      121 tcgccctgcg ccagccgggc cgtgcccagc aggccggccg cctgcggtcc cagcggatcg
      181 cggcggacca gcgcttcggc cagtggtgca atctggccag cctggcctgc cagcagcagc
      241 gcctggcctt ggctggacaa ggcttcttgc cggaaaggtt caggaatgcg cgctgcagcc
      301 ccgggatcag aggcggcagt gcgatcgaag gccagcgcga tgctcagccc ggcatagccg
      361 gctgccgcaa tcacgactgc ggcaaggacc gccttccgcc gatcagcggt gtgcggtccg
      421 gccatcctag tcgcgctccc ggtcgctctg ttcgtaggtg tagtactggt agccgtagta
      481 ttcggaatag cggttgcccg aacgggtcgg gtcaaacttg gtcagcaccg cgcccagcag
      541 gatcggccgc attgcgcgca ggcggcgcag cgcagtcttg agcgagccgc ggcggctgcg
      601 atcgctttcg accacgaata tcaccccgtc ggccagcgcc gacagtgccg gcgcatcggc
      661 caggcccagg atcggcgggc tgtcgatcac caccacgtca taggtgcggg ccagttcctg
      721 gacgacatgt tccatccgcg gcgaggagac cagctcggtc gggctgggcg ggatcgggcc
      781 cgaggcgatg acgaacaggt tttcctgcgg cgtggccaga atcgcgcttt caaccggatc
      841 gcccgaggtc aacaggtcgg tcaggccgcg atcgttgggc aggccaaagc ggcgatggat
      901 cgaggggcgc cgcaagtcgg catcgatcag cgccaccttt cggcccatcc gcgccaggct
      961 ggcggcggtg gcgaagctgg tggtcgtctt gccttcggcc gactgtgccg aagtgaccag
     1021 catgacctgc ggcagaccgg ccgaagtcga atagagcagc gccgcgcgca gcgagttgta
     1081 ggcttcggaa accggcgact tgggatcgcc catttcactg tcgggatcgc ggccggtggc
     1141 cttggggacc acacccagca agggcagacg cagcttcgat tcgacgtctt ccggcacccg
     1201 gatcgcgtcg tcgagctgat ccttgatgaa cagcgtcagc gcagcaagac caacgccgac
     1261 cagcagcgcc agcagcaggt tcttcagcat gttgggtgag gtcgggatag tcggccgttc
     1321 ggcactgtcg atgatcgaga cgttgctcga ggcgatcccg gccgtggcgt taagctcgtt
     1381 atagcgcttc agcagcccgt catagagcgt gcggttggta tcggcctcgc gcttcagcag
     1441 accatattgc acggtcttgt cctgttcgcg cagggtctca cccttcaggt tttccagctt
     1501 ggccagcagg gcctgttcgg tgccctgcgc cgccagcatt tcggcgcgga tcgagttgcg
     1561 caccgattgc gcggccgatt gcagttccgc atcgagccgg gccagctgcg cccgcttttc
     1621 gcgcacggtc ggatagtctt cgaggtgctt ggacagctcg cgctgcagtt cggcctgggt
     1681 ggtggcgcga tcggtcatca ggcccgaaac ggttgcgttg cccagcactt cgcggctcga
     1741 cagcagcggg accgatgaaa tcgcccgcca gcgcccttcg gccgcaaccc gctcggcagt
     1801 ggcctggttg gccgcctggt taagttgcaa caggcttgcc gcggtcaccg atccacccgt
     1861 ggtcgcctcg cccgtttcgc cggcggcgtc gccagtccgg atcaggccgg tgctgcgcga
     1921 atagtcgttg agattgcgtt cggcctgctc aacccgctgc ttggcttcgg ccatctggtt
     1981 ggccacgaat tcgcgggcgt aggacgaact gtcgaacttg cgctgcaggt tcgactggat
     2041 gaattcagtc gcaaaggcat tggcgatttc ggccgacatg ctggggtcgg cgctttcgaa
     2101 cgagatcgtg acgatccgcg aatcgcgcgg cagattgacc ttcaggttgc ccagcagcag
     2161 gcccaccacc tggttgcgca tcgcctcggc cgtgacgccg gcgccgggcg ccttggattc
     2221 catcgcggca tagaactcgg gacgggtctg gagcttcagc ttttgcgcca cccgctgggc
     2281 caggccgcgg ctgcgcagga tatcggtctg cgtcttgaga aagcggtcaa cgtcgtaaag
     2341 gttcgattcc tgcgcggtct gatcgtcatt ctcaccgatc acgcgatcgc tgctatcgtt
     2401 gatctggatc gtcgtattgg cggtataacg gggcgtgtcg agcagcgtga tgaccagcgc
     2461 cgcacccagc gcaaccagga tgatcgcgct gatcagcagc aggttgctgc ggatcgctgc
     2521 ggcaatgtag cgcaggtcaa aggtcccgcg tgtcatcgcc tggaagactt ccggctcgtc
     2581 gtgcaggtca accgaattgg tcggctggat atcgcgcagg atggggtgat cgttcatgac
     2641 aggatcgagg ctccgccgag gctgcgcatt cagcgaacca ggtagaagag attgagcagc
     2701 ggggcagtct cgcgcacatc
//
LOCUS       DASUDV010000325         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_761648,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000325 DASUDV010000000
VERSION     DASUDV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761648"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /locus_tag="VFV30_07080"
     CDS             complement(<1..83)
                     /locus_tag="VFV30_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX4847891.1"
                     /translation="MKRIAVYCGSATPADPRYLEVAREVGET"
     gene            complement(80..1579)
                     /gene="crtI"
                     /locus_tag="VFV30_07085"
     CDS             complement(80..1579)
                     /gene="crtI"
                     /locus_tag="VFV30_07085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX4847892.1"
                     /translation="MPATRSEQPRACVIGAGLGGLALAIRLQTAGMATTLVEARERVG
                     GSCWAHEQDGFRFEEGPSLITDPAPLRELWALAGRDMAESISLCEVTPHCRFSWPDGT
                     TFDLSGDDAAMAREVARFAPGDLAGFEQFRRWCDEAQRDGCGQIGEGLLASPALIGRA
                     AAPFVRYQGWRSAHGLVSHFVRNERLRQALSFAALQSGGNPMRIGAFALLSHRIGRGQ
                     SGWWPEGGMSALAAAMAGLFEQLGGTIRLHDPVIAIHTLGNRAHEVETQSGWRQHCGL
                     VASNADVVHTYRDLIGASQRGADMARKLADRRYSPGLFTVHLGLEGSWPGIPHSSVLM
                     GPRYAGLFADVFDHGVLPQDFVLMLDHPSITDPTLAPPGKSVLRAAIPVANLRRLPID
                     WESHGTLIENRIIAEIGRRLVPDIEDRIVTRRHTSPRDAVLDRNVYGGSAWSLEPGQL
                     WSGRPRPANRDGRIANLYLVGSATNPGAGLPATLASAKASARLILENHR"
     gene            1666..2196
                     /locus_tag="VFV30_07090"
     CDS             1666..2196
                     /locus_tag="VFV30_07090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4847893.1"
                     /translation="MRIGPASLDDVPEITAIYAHHVLNGTASYETVPPGESEIAARME
                     KCMSAGWPWLVGRDDAGTVLGYAYVNQFRDRAAYRLTCENSIYIRHDRRGEGIGRALL
                     ALLLVEAERAGFRQMIAVIGGAEPASIRLHGACGFTQAGRLEAIGRKHGRWLDTVYMQ
                     RALGEGSATPPAEEPA"
     gene            2193..2792
                     /locus_tag="VFV30_07095"
     CDS             2193..2792
                     /locus_tag="VFV30_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HEX4847894.1"
                     /translation="MSKPADSLLPHRPALILALLIAAYGIFILFAMGRPPICECGVVR
                     LWTGAVNSPENSQQLADWYTFSHIIHGMIFYAGAHLLWRRWKLFGGAPTRWAFPIAVA
                     LEASWELLENSPMIIERYRSVTVNFGYAGDSIINSAADIVWMSLGFLIAARLNWKATL
                     ALAIGFEMMTLAVIHDNLTLNVLMLVYPLDAVRAWQGTV"
BASE COUNT          472 a          937 c          935 g          485 t
ORIGIN      
        1 gtttcgccca cttcgcgcgc cacttcgaga tagcgcggat cggctggcgt tgcggaaccg
       61 caatagacgg caatgcgctt catcggtgat tctccaggat cagtcgggcg ctcgccttgg
      121 cgctggccag cgtggctggc aagcccgcac ccgggttggt cgcgctgccg accaggtaga
      181 gattggcgat ccgcccatcg cggttggcgg ggcgggggcg gccggaccac agctgaccag
      241 gctcaaggct ccaggccgaa ccgccataga cattgcgatc gagcaccgcg tcgcgcgggc
      301 tggtgtggcg tcgggtgacg atccgatcct cgatatcggg caccagccgg cgcccgattt
      361 cggcaatgat ccggttctcg atcagggtgc cgtggctttc ccagtcaatc ggcagacggc
      421 gcaggtttgc caccgggatc gcagcgcgca gcacgctttt gcccgggggt gccagcgttg
      481 gatcggtgat gctggggtga tcgagcatca gcacgaagtc ctggggcagc accccatgat
      541 cgaacacgtc ggcaaacagc ccggcatagc gcggacccat cagcacgctg gagtggggta
      601 tgcccggcca gctgccctcc aggccaagat gcacggtgaa caggcccggg ctgtacctcc
      661 ggtccgccag tttgcgcgcc atgtcggcgc cgcgctggct ggcgccgatc agatcgcgat
      721 aggtgtgcac cacatcggca ttgctggcca ccagcccgca gtgctgccgc cagccgcttt
      781 gcgtttccac ctcgtgcgcg cgattgccca gcgtgtggat cgcaatcacc gggtcgtgca
      841 ggcggatcgt tccgcccagt tgctcgaaca ggccggccat ggcagccgcc agcgcgctca
      901 tcccgccttc gggccaccag ccggactgac cgcgcccgat acggtgcgac agcaaggcaa
      961 aggcgccgat ccgcatcgga ttgcccccac tctgcaaggc ggcaaaggac agcgcctggc
     1021 gcagccgttc gttgcggacg aagtggctca ccaggccatg ggcgctgcgc cagccctggt
     1081 agcgcacgaa aggcgcggct gcgcggccga tcagcgctgg cgatgccagc aggccttcgc
     1141 cgatctgtcc gcatccgtcg cgctgcgcct catcgcacca gcgccggaac tgctcgaagc
     1201 cggcaagatc accaggcgcg aagcgggcca cctcgcgcgc catcgccgca tcgtcgcccg
     1261 acagatcgaa ggtggtgcca tcgggccagc tgaagcggca atggggggtc acttcgcaca
     1321 gggaaatgct ctcggccatg tcgcgtccgg ccagcgccca caattcgcgc aagggcgccg
     1381 gatcggtgat cagcgatggc ccttcttcga agcggaaacc gtcctgttca tgcgcccagc
     1441 agctcccgcc gacgcgttcg cgcgcctcga ccagcgtcgt cgccatcccg gcggtctgga
     1501 gccggattgc cagggccagc ccgcccagcc cggcgccgat cacgcaggcg cgcggctgtt
     1561 cggatctggt tgcgggcatg gtcatcgccc gcgcggtttt agtgatggtg ggcaatcatg
     1621 caacgccctt ggcgaagccc acaggatagg tcattgtggg ccgcgatgcg gataggacct
     1681 gccagcctcg acgatgtgcc tgagatcacg gcgatctatg cccatcacgt gctgaacggt
     1741 accgcatcct acgaaaccgt cccgccgggc gaatccgaga tcgccgcacg gatggagaaa
     1801 tgcatgagcg ccggctggcc atggctggtc gggcgcgacg atgccggaac ggtgctgggc
     1861 tatgcctatg tcaaccagtt ccgcgaccgc gcggcctacc gcctgacctg cgagaattcg
     1921 atctacatcc gccatgaccg gcgcggcgag ggaatcggcc gggcactcct ggcgctgctg
     1981 ctggtcgaag cggaacgcgc cggctttcgc cagatgatcg cggtgatcgg tggggccgaa
     2041 ccggcctcga tccgcctgca tggcgcctgc ggctttaccc aggccggtcg gctggaagcg
     2101 atcgggcgca agcacggccg ctggctcgat acggtctaca tgcagcgcgc gcttggcgaa
     2161 ggcagcgcga ccccgcccgc ggaggaacca gcttgagcaa accagccgac agtctcctgc
     2221 cacaccgccc ggcgctgatc ctggccctgt tgatcgccgc ctatggcatc ttcatcctgt
     2281 ttgcgatggg ccgtccgccg atctgcgaat gcggggtggt caggctgtgg accggggcgg
     2341 tcaactcgcc cgaaaacagc cagcaactgg ccgactggta taccttcagc catatcatcc
     2401 acggaatgat cttctatgcc ggggcgcacc tgttgtggcg gcgctggaaa ctgtttggcg
     2461 gggcgccaac ccgctgggcc tttccgatcg cggtcgcgct cgaggcgagc tgggagctgc
     2521 tggaaaattc gccgatgatt atcgagcgct atcgctcggt cacggtcaat ttcggttacg
     2581 ctggcgatag catcatcaat tcggcggccg atatcgtctg gatgtcgctg ggtttcctta
     2641 tcgccgcgcg gctgaactgg aaggcaaccc tggctttggc gatcggcttc gagatgatga
     2701 cgcttgcggt gatccacgac aacctgacac tcaacgtgct gatgctggtc tacccgctcg
     2761 atgcggtcag ggcctggcag ggcacggttt gacgcgcatc aattaccctc ttggcaagtc
     2821 cggcaataa
//
LOCUS       DASUDV010000326         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1026607,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000326 DASUDV010000000
VERSION     DASUDV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026607"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="trpS"
                     /locus_tag="VFV30_07100"
     CDS             <1..979
                     /gene="trpS"
                     /locus_tag="VFV30_07100"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX4847895.1"
                     /translation="LGNYLGAIRNYVRLQDEAEAAGGQSLYFLADLHAISQPHVPAEL
                     KQNTLEMTAALVACGIDPDRSILFNQAQVPQHAELQWLLTGTARMGWLNRMTQWKDKA
                     GKNREGQSVALFTYPVLQAADVLLYQATHVPVGEDQKQHLELARDIAQKFNNDFASED
                     APVFTLPDPIIPPEAARIMSLRDGSAKMSKSDPSDMSRINLTDDADTIMQKVKKAKTD
                     PEPLPGDKGGLEGRPEAANLVGIYAAMAGESQDAVLARFGGEGFGKFKPALGELLVES
                     LAPINARFLDLRQDREALDAILAKGALKARELAAPTLTAAYDALGLVRG"
     gene            1120..1803
                     /locus_tag="VFV30_07105"
     CDS             1120..1803
                     /locus_tag="VFV30_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX4847896.1"
                     /translation="MDHNTRSPFAWLKLALVPLALATLAACATPFRADVQRFSAQLPA
                     PQGQTYAVVAEDPSLAGGLEFAQYAALVDAKMTGLGYVRAADPANADLLVKFDYGVDR
                     GRTQVRSSAFADPYWSSWYGYPRWGWGRRPYSPFYPRGVWGYGWYDPWFNPGYESYTV
                     YLSAVSLKIDRRADGLRLFEGRAEAASTSNRLGYLVPNLVEAMFTGFPGNSGETVRIS
                     VAPEKKKKN"
     gene            complement(1880..>2171)
                     /gene="dut"
                     /locus_tag="VFV30_07110"
     CDS             complement(1880..>2171)
                     /gene="dut"
                     /locus_tag="VFV30_07110"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX4847897.1"
                     /translation="IPDGYEIQVRPRSGLALKHGITVPNTPGTIDSDYRGEVKVILIN
                     LGDAPFDIRRGDRVAQLVLAPVTRASWLPVEDLDETARGAGGFGSTGGVVAL"
BASE COUNT          370 a          749 c          718 g          334 t
ORIGIN      
        1 ccttggcaat tacctggggg cgatccgcaa ctatgtgcgc ctgcaggacg aagccgaggc
       61 ggccggcggg cagagcctct atttcctggc cgacctccac gccatcagcc agccgcacgt
      121 gccggccgaa ctgaagcaga acacgctgga aatgaccgcc gcgctggtcg cctgcgggat
      181 cgatcccgat cgctcgatcc tgttcaacca ggcccaggtt ccgcagcatg ccgaactgca
      241 atggctgctg accggcaccg cccggatggg ctggctgaac cggatgaccc agtggaagga
      301 caaggccggc aagaaccgcg aaggccaatc ggtcgcgctg ttcacctatc ccgtgctgca
      361 ggccgccgac gtgctgctgt accaggccac ccacgtgccg gtgggcgagg accagaagca
      421 gcacctggag ctggcccgcg acatcgcgca gaagttcaac aacgacttcg cgagcgagga
      481 cgccccggtc ttcaccctgc ccgatccgat catcccgccc gaggcggccc ggatcatgag
      541 cctgcgcgac ggcagcgcca agatgagcaa gtccgatccg agcgacatga gccggatcaa
      601 cctgaccgac gatgccgaca cgatcatgca gaaggtcaag aaggccaaga ccgatcccga
      661 gccgctgccc ggcgacaagg gcgggctgga aggccggccg gaagccgcca atctggtcgg
      721 catctatgcg gcgatggccg gggaaagcca ggacgcggtg ctcgcccggt tcggcggcga
      781 aggctttggc aagttcaagc cggcgctggg cgagctgctg gtcgaaagcc tggccccgat
      841 caacgcacgg ttcctggacc tgcggcaaga ccgtgaggcg ctcgacgcaa tcctggccaa
      901 gggcgcgctc aaggcgcgcg agctggccgc gccgacgctg acggccgcct atgacgcgct
      961 gggcctggtc cggggctgat cggccgcgct cccgatccgg gacaggcgcc attcaagcac
     1021 gattaagccg gcttggcgca ccctgcccgg acaattagac ttttcgtcat tccgcgcgcg
     1081 agtcgcgcgc cgattggaca ccaactgggg atcgcagcga tggaccacaa cacccgttct
     1141 cccttcgcct ggctgaagct ggccctggtg ccgctggccc tggccacgct ggctgcctgc
     1201 gccacgccgt tccgggcgga tgtgcagcgc ttttccgccc agctgcccgc gccgcagggc
     1261 cagacctatg ccgtggtggc cgaggatccc tcgctcgccg gggggctgga attcgcgcag
     1321 tacgccgcgc tggtcgacgc caagatgacc ggcctgggct atgtccgcgc ggccgatccg
     1381 gcgaatgccg atctgctggt caagttcgat tacggggtgg accgcggccg cacccaggtc
     1441 cgctccagcg cctttgccga tccctattgg tcgagctggt acggctatcc ccgctggggc
     1501 tggggccgcc ggccctattc gccgttctac ccgcgcgggg tctggggcta tggctggtac
     1561 gatccgtggt tcaacccggg ctatgaaagc tacaccgtct atctcagcgc ggtcagcctg
     1621 aagatcgacc gccgcgccga tggcctgcgc ctgttcgaag gccgcgccga agccgcctcc
     1681 acctccaacc ggctgggcta tctggtgccc aacctggtcg aggcgatgtt caccggcttc
     1741 ccgggcaatt ccggcgaaac ggtccgcatc tctgtggcgc cggaaaagaa gaagaagaac
     1801 tgatccggca gcttcgccgg tcacggtctg aaacgcagca agggcgggtc cggaaacgga
     1861 tccgcccttt cgcttggggc tagagcgcca ccaccccgcc ggtgctgcca aagccacccg
     1921 ccccgcgcgc ggtttcgtcc agatcctcga ccggcagcca gctggcccgg gtgacggggg
     1981 ccagcaccaa ctgcgccacc cggtcgcccc ggcggatgtc gaagggagcg tcgcccaggt
     2041 tgatcaggat gaccttcact tcgccgcggt aatcgctgtc gatcgtgccg ggggtgttgg
     2101 gcacggtgat cccgtgcttc agcgccaggc cagagcgcgg gcggacctgg atttcatagc
     2161 catcgggaat c
//
LOCUS       DASUDV010000327         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1380060,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000327 DASUDV010000000
VERSION     DASUDV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380060"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /gene="lptD"
                     /locus_tag="VFV30_07115"
     CDS             complement(<1..1248)
                     /gene="lptD"
                     /locus_tag="VFV30_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX4847898.1"
                     /translation="MLLPARPHLPQSLLCPSSARAGAAALALALLCLPGAALAQAAPA
                     EPPASAEPVLPEGGTIDFEADGASYDDKADRIVVSGNVVLRRGDQSVRADRIVWDRTT
                     GTIMAEGNIRYVDEDGNQLFTERLELTDELKAGAMDNMLLALREGGRLAANKGERLED
                     GKLLLHNAAYTACAVEDDQGCPRNPTWRITARRVIHDPEKKTVRFEGARAEVFGIKLL
                     PMPGLVLATDGRPISGLLIPDVQLSASNGIQISDTWYQRLSANRDLAVTGYLFTEALP
                     MVSAQYRQLTGTGAYQVTGYLTRSSRIPIGSSSTVSQPDWRGYLATNGRFQFTPNWSL
                     TFSGRLASDRTFLRRYDISRDDRLRSMAELERIDADSYLSVSGWATQTLRVGDAQGQV
                     PFAIPVIDYRRRLTDPWLGGRIQL"
     gene            1347..>2224
                     /locus_tag="VFV30_07120"
     CDS             1347..>2224
                     /locus_tag="VFV30_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4847899.1"
                     /translation="MNVEFLSGPVSPSASPIAYVVDQGNLPAGLEPVVAEGAAASRFA
                     GKPGQCHEAFVTRDGAVVRLALAGAGEPGSKDRSAALERAGAALTAKYLASGETALTI
                     DFTGTRMTGGEAASVLLGARLRGWRYDTYRTRLPEEQKVSLKTIKVAGAPEGTEAAWA
                     VEAALAEGIEFTRELVTEPANVIYPASFVERAHQRLDGTGVRIRVLDRDEMAALGMGA
                     LLGVAQGSVREPKLLVMEWNGGAAGEKPTAFVGKGVTFDTGGISLKPPGGMEDMKWDM
                     GGAGAVAGAMLALAKR"
BASE COUNT          356 a          762 c          783 g          323 t
ORIGIN      
        1 cagctggatc cgcccgccca gccacggatc ggtcaggcgg cggcggtaat cgatcaccgg
       61 aatcgcgaag ggcacctggc cctgcgcatc gccgacccgc agcgtctgcg tcgcccagcc
      121 ggaaaccgac aggtagctgt cagcatcgat ccgctccaac tctgccatcg agcgcagccg
      181 gtcatcccgg ctgatgtcgt agcggcgcag gaaggtgcgg tcggaggcca gccggccgga
      241 gaaggtcagg ctccaattcg gggtgaactg gaagcggccg ttggtggcaa gatagccgcg
      301 ccagtcgggc tggctaaccg tgctgctgct gccgatcgga atgcgcgagg agcgggtgag
      361 gtagccggtc acctgatagg ctccggtgcc ggtcagctgg cggtactgcg ccgaaaccat
      421 cggcaacgcc tcggtaaaga gatagccggt caccgccaga tcgcggttgg ccgacaagcg
      481 ctggtaccag gtgtcggaaa tctggatccc gttcgatgcc gacagctgga catcggggat
      541 cagcaggccg gaaatcgggc gcccgtcggt cgccaggacc agccccggca tcggcagcag
      601 cttgatcccg aagacttccg cccgcgcgcc ttcaaagcgc accgtcttct tttccggatc
      661 gtggatcacc cgccgcgcgg tgatccgcca ggtgggattg cggggacagc cctggtcgtc
      721 ctccaccgcg caggcggtgt aggccgcgtt gtggagcagc agcttgccgt cctccagccg
      781 ctcgcccttg ttggccgcca gccgcccgcc ttcgcgcagc gccagcagca tgttgtccat
      841 cgccccggcc ttcagctcgt cggtcagctc cagccgttcg gtgaacagct ggttcccgtc
      901 ctcgtcgaca tagcggatgt tgccttcggc catgatcgtg ccggtggtgc ggtcccacac
      961 gatccggtcc gcccggaccg actggtcgcc ccggcgcagc accacgttgc ccgaaaccac
     1021 gatccggtcg gccttgtcgt catagctggc gccgtcggcc tcgaaatcga tcgtgccgcc
     1081 ttcgggcagg accggctcgg ccgaagcggg cggctcggcc ggggcggcct gggccagcgc
     1141 cgcgcccggc aggcacagca gggccagggc cagggcggcg gctccggcgc gcgccgaaga
     1201 ggggcatagc aaagattgcg gcaaatgagg ccgcgcgggc aggagcatgg cttgcctatc
     1261 gcaccgcccg cgcctaactg caatccacgc caacatcctg cggcaacgct ttgtcgcagc
     1321 gcaaagcctt tccccaggga gctaccatga acgtcgaatt cctctccggc cccgtttcgc
     1381 ccagcgccag cccgatcgcc tatgtggtgg atcagggcaa tctgcccgcc gggctggagc
     1441 ctgtcgtggc cgaaggcgcc gcggccagcc gctttgccgg caagcccggc cagtgtcacg
     1501 aagccttcgt cacccgcgac ggcgcggtgg tccgcctcgc gctcgccggt gcgggtgagc
     1561 cgggcagcaa ggaccgctcc gccgcgctgg aacgcgccgg cgcggcgctg acggcgaagt
     1621 atctcgcctc gggcgaaacc gcgctgacca tcgatttcac cggcacgcgg atgaccggcg
     1681 gcgaagcggc atcggtgctg ctgggcgcgc ggctgcgcgg ctggcgctat gacacctacc
     1741 gcacccgcct gcccgaagag cagaaggtca gtctcaagac catcaaggtc gccggtgcgc
     1801 ccgaagggac cgaagcggcc tgggccgtcg aagcggcgct ggccgaaggg atcgagttca
     1861 cccgcgaact ggtgaccgaa cccgccaacg tgatctaccc cgccagcttc gtggagcgcg
     1921 cgcaccagcg gctggacgga accggcgtgc ggatccgggt gctggaccgc gacgagatgg
     1981 ccgcgctcgg catgggcgcg ctactgggcg tggcgcaggg ttcggtgcgc gagcccaagc
     2041 tgttggtgat ggaatggaac ggcggcgcgg ccggggaaaa gcccaccgcc ttcgtcggca
     2101 agggcgtgac ctttgacacc ggcgggatca gcctgaagcc gccgggcggc atggaagaca
     2161 tgaagtggga catgggcggg gccggtgcgg tcgccggggc catgctcgcg ctggccaagc
     2221 gcaa
//
LOCUS       DASUDV010000328         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_805727,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000328 DASUDV010000000
VERSION     DASUDV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805727"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFV30_07125"
     CDS             complement(<1..1178)
                     /locus_tag="VFV30_07125"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX4847900.1"
                     /translation="MPRPSDLILTAAHMRAAEEALIAAGTSVDELMQRAGQGAADWVW
                     RISGGRAVTVLCGPGNNGGDGWVIAETLRQRGSDVAVVMAAEPATDAARTARALYKGQ
                     VLLPDAERHGEVLVDCLFGTGLTRPLSEDHLALMQRLAASHDKRIAVDLPSGVSSDDG
                     ALLSDGLPHNDLTIALGAWKHAHYVMPAAARMGALRRVDIGITPVRNDFTMPWVLRRP
                     VLTPPAADANKYSRGMVGIVVGDMPGAALLAAKAAMASGAGYVKLLSDGQAVAAPADL
                     VVDDRPLGQAVTDHRLDAILVGPGLGRDARAQQRLNTVLQSCQHIAVVLDADALHLYP
                     HSEWSGPDSGTYDGSRHSLLSCFEMHSKAEPSRPQGLDPSDVALLRRQIVMTPHQGEL
                     "
     gene            complement(1162..1524)
                     /locus_tag="VFV30_07130"
     CDS             complement(1162..1524)
                     /locus_tag="VFV30_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847901.1"
                     /translation="MRAAALALTLTVAACSGAPAGDPLPQGEEVFCALGGSQEFKAQC
                     RVERTAVDGAKVIVVRHPDGGFRRLEVSKDGQNLLAADGADQSQSALKGDRYEVILGD
                     DRYVIPANPPAAGDAAPK"
     gene            complement(1524..>3123)
                     /gene="ilvD"
                     /locus_tag="VFV30_07135"
     CDS             complement(1524..>3123)
                     /gene="ilvD"
                     /locus_tag="VFV30_07135"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4847902.1"
                     /translation="GHDGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGM
                     LMAAMRINIPVVFVSGGPMEAGKVVLRGKEVALDLVDAMVAAADDSYTDEEVTEIERS
                     ACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSVLATHADRQQLFLRAGRLAVELCKR
                     YYEGDDDSVLPRSIGSFEAFENAVALDIAMGGSTNTVLHLLAAAHEAGLDFTMKDIDR
                     LSRKVPCLSKVAPAKSDVHMEDVHRAGGIMAILGELDRAGLLHTHLPTVHSKTMGDAL
                     NQWDIARTQDPAVEHFYKAAPGGVPTQVAFSQDRRWKELDKDRETGVIRSKEHAFSQD
                     GGLAVLYGNIALDGCIVKTAGVDDKILKFTGPAKVYESQDAAVTAILTGQIVAGDVVV
                     IRYEGPKGGPGMQEMLYPTSYLKSKGLGAACALLTDGRFSGGTSGLSIGHASPEAAEG
                     GAIGLVETGDTIEIDIPNRTINLAVSDEELDRRRAAQDAKGWQPAKERPRKVTPALQA
                     YAAMTTSAARGAVRDVTQIMRKPG"
BASE COUNT          467 a         1095 c         1022 g          539 t
ORIGIN      
        1 tcgagttcgc cctggtgcgg ggtcatcacg atctggcggc ggagcaaggc aacgtcggat
       61 gggtccagac cctgcggtct ggatggctcc gctttgctgt gcatttcgaa gcaggaaagc
      121 agcgaatggc gggagccgtc ataagtgccc gagtccggac cgctccattc gctgtgcgga
      181 tagagatgca gcgcatccgc atcgaggacc acagcgatgt gctggcagct ttgcagcacc
      241 gtgttcaagc gctgctgtgc gcgcgcatcg cggcccagtc ccggtcccac cagaatggca
      301 tcgagccggt ggtcggtcac cgcttggccg agcggcctgt catccaccac cagatcggcc
      361 ggtgccgcga cagcctggcc atcgctcagc agcttcacat agcccgcgcc gctggccatt
      421 gcggccttgg ccgccagcaa ggccgcaccc ggcatatcgc caacgacgat accgaccatg
      481 ccgcgcgaat acttgttggc atccgctgcc ggaggcgtca gcaccggacg ccgcagcacc
      541 cagggcatgg tgaaatcgtt gcgcacgggc gtgatgccga tgtccactcg ccgcaacgcg
      601 cccatccggg ccgcggcagg catcacgtag tgcgcgtgtt tccaggcccc cagcgcaatc
      661 gtcaggtcgt tgtgcggcag gccatcgctc agcaacgcgc catcgtcact cgaaaccccg
      721 ctgggcagat cgaccgcgat ccgtttgtca tggctggccg ccagcctttg catcagcgcc
      781 agatggtcct cgctcagcgg ccgggtcagg ccggtgccga acaggcaatc gaccaacacc
      841 tcgccgtggc gttcggcatc cgggaggaga acctgtccct tgtaaagcgc ccgcgccgtc
      901 cgcgccgcat cggtcgcagg ctccgcggcc atgaccaccg ccacgtcgct gccgcgctgg
      961 cggagcgttt cggcgatcac ccagccatcg ccgccgttgt tgccggggcc gcacagcacg
     1021 gtcaccgccc gcccgccgga gatccgccag acccagtccg ccgcgccctg cccggcgcgt
     1081 tgcatcagtt cgtccacgct ggtgccggca gcgatcagcg cctcttccgc cgcgcgcatg
     1141 tgcgccgcgg tcaggatcag gtcacttggg cgcggcatcg cccgcggccg gcgggttggc
     1201 ggggatgaca tagcggtcat cccccaggat cacttcatag cgatcgccct tcagcgccga
     1261 ctggctctgg tccgcgccat cggcggcgag caggttctgc ccgtccttgc tcacttccag
     1321 acgccggaaa ccgccatcgg ggtggcgcac cacgatcacc ttcgcgccgt ctaccgcggt
     1381 gcgctccacc cggcactggg ccttgaattc ctgcgatccg cccagcgcgc agaacacctc
     1441 ttccccctgc ggcagcggat cgcccgccgg tgccccggaa caggccgcga ccgtgagggt
     1501 cagggccagc gcggcggccc gcatcagcca ggtttccgca tgatctgggt tacatcccgt
     1561 acggccccgc gcgcggcgct ggtggtcatc gcggcataag cctgcagcgc cggagtcacc
     1621 ttgcgcgggc gctccttggc gggctgccag cccttggcat cctgcgcggc gcggcggcgg
     1681 tcaagctcct cgtcgctgac ggccaggttg atggtccggt tcggaatgtc gatctcgatc
     1741 gtgtcgccgg tttcgaccag cccgatcgcc ccgccctcgg ccgcttcggg cgaggcatgg
     1801 ccgatcgaca ggcccgaggt tccgccggaa aagcgcccat cggtcagcag cgcgcaggcc
     1861 gcgcccagcc ccttcgactt gaggtagctg gtgggataga gcatttcctg catgccgggg
     1921 ccgcccttgg gcccttcata gcggatcacc accacgtccc cggcgacgat ctgtccagtg
     1981 aggatcgcgg tgacggcggc gtcctggctt tcatagacct tggccggacc ggtgaatttc
     2041 aggatcttgt cgtccacccc ggcggttttc acgatgcagc cgtcgagcgc gatgttgcca
     2101 tagagcacgg ccagcccgcc atcctggctg aaggcgtgtt ccttgctgcg gatcacgccg
     2161 gtttcgcggt ccttgtccag ttccttccag cgccggtcct ggctgaaggc aacctgggtc
     2221 ggcacgccgc ccggcgccgc cttgtagaag tgttcgaccg ccggatcctg ggtgcgggca
     2281 atgtcccact ggttcagcgc atcgcccatg gtcttgctgt gcacggtcgg caggtgggtg
     2341 tggagcagac cggcgcggtc cagctcgccc aggatcgcca tgatcccgcc ggcgcggtgg
     2401 acgtcttcca tgtggacgtc gctcttggcc ggggcgacct tgctgaggca cggcaccttg
     2461 cggctcagcc ggtcgatgtc cttcatggtg aagtccagcc cggcctcgtg cgcggcggcg
     2521 agcaggtgga gcacggtgtt ggtcgacccg cccatcgcga tatcgagcgc gacggcgttt
     2581 tcgaacgctt cgaagctgcc gatgctgcgc ggcaggacgc tgtcgtcgtc gccctcgtag
     2641 tagcgcttgc acagctccac cgccaggcgg ccggccctca ggaacagctg ctggcggtcg
     2701 gcatgggtcg ccaggaccga gccgttgccg ggcagcgaga ggcccagcgc ctcggtcagg
     2761 cagttcatcg agttggcggt gaacatgccg gaacaggacc cgcaggtcgg gcaggcggag
     2821 cgttcgatct ccgtcacttc ctcgtcggtg tagctgtcat cggcggcggc aaccatggca
     2881 tcgaccagat cgagcgccac ttccttgccg cgcagaacca ccttgccggc ttccatcggc
     2941 ccgccggaca cgaagaccac cggaatgttg atccgcattg ccgccatcag catgccgggc
     3001 gtgatcttgt cgcagttgga aatgcagacc atcgcatcgg cgcagtgggc gttgaccatg
     3061 tattcaacgc tgtcggcaat cagatcgcgg ctgggcagcg aatagagcat cccgtcgtgg
     3121 ccc
//
LOCUS       DASUDV010000329         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1557109,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000329 DASUDV010000000
VERSION     DASUDV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557109"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VFV30_07140"
     CDS             <1..1092
                     /locus_tag="VFV30_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEX4847903.1"
                     /translation="FGLAAHWAYKSHERPDGQVGWLRDLIEIVDASHDAEELLEHTRL
                     AIYQDRIFAFTPKGQLLQLPKGSTPVDFAYAVHSDLGSFAVGAKINGRHMPLRTALGN
                     GDVVEIIKSRNASPQLSWLGFVVTGKARAAIRRAVRAKEREEVAMIGRKLFDEIALRL
                     PSKIGKKAIRDAVKRLGLASDTELMIAIGTAKIDDRQVMEALVPGSTSHMPEPEHWPR
                     QERAISIRGLTAGMAFHLGDCCHPVPGDRIVGIRQPEKGVEVHSIDCRTLASGVDADW
                     LDLSWGERTTGAVGRLRMVLYNRPGTLAEAAGIFAGNRANIINLENTGRDELFGTYEI
                     DLEVNDLAHLTRIVSALRASDAVAEAERI"
     gene            complement(1148..1459)
                     /locus_tag="VFV30_07145"
     CDS             complement(1148..1459)
                     /locus_tag="VFV30_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEX4847904.1"
                     /translation="MTETITHSGPLWRWTGSSNGTWHFLTIDGEAGEALSETALMRRL
                     EKSIGGFGSLKVRARIGDSTFNTSLFPSKNLGWLLPVKASVRKAEGVSEGDLVEVVLE
                     V"
     gene            complement(1621..2670)
                     /locus_tag="VFV30_07150"
     CDS             complement(1621..2670)
                     /locus_tag="VFV30_07150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4847905.1"
                     /translation="MHKSLIALAALAVATPALPQASAAIASGKASPSPLAPSEIVARA
                     PKTEWMAIAQSDLLVMDLSPDAKGKPRRVVIQLMPAPFSQGWIGNIRKLAAARFWDGT
                     SINRVQDNYVVQWGDPEAEDKARAKALPAGLASVPQSEYQFRSEEVGSIMMERARAWK
                     VAATPNVLQRTIEGEVIDLQDLRNNPAGIPDPYAPFAGIHHGWPLATDGTNNWPVHCY
                     GMVGVGRDMPPSTGTGAELYTVIGHAPRHLDRNIALVGRVIEGIEHLSSLPRGHGDLG
                     FYDLAQGDQRVTIQSIRIGTEVNDLPAYEYLSTESASFAAYADARANRRDAFFIRPAG
                     GADICNVPVPVRRAK"
BASE COUNT          460 a          896 c          870 g          491 t
ORIGIN      
        1 ttcgggcttg ccgcgcactg ggcctacaag agccacgagc gccccgatgg ccaggttggc
       61 tggctgcgcg acctgatcga gattgtcgat gccagccacg atgccgagga actgctcgag
      121 catacgcggc tcgcgatcta ccaggaccgg atcttcgcct tcacccccaa gggtcagctc
      181 ctgcagctgc ccaagggttc caccccggtc gatttcgcct atgcggtcca ttccgacctc
      241 ggcagcttcg cggttggtgc caagatcaac ggccgccaca tgccgctgcg caccgcgctg
      301 ggcaacggcg acgtggtgga gatcatcaag agccgcaatg cctcgccgca gctgtcgtgg
      361 ctcggcttcg tcgttaccgg caaggcgcgg gccgcgatcc gccgcgcggt gcgcgccaag
      421 gagcgcgagg aagtggccat gatcggccgc aagctgttcg acgagatcgc gctgcgcctg
      481 ccctcgaaga tcggcaagaa ggcgatccgc gatgcggtga agcggctggg gctggcgagc
      541 gataccgaac tgatgatcgc gatcggcacc gccaagatcg acgatcgtca ggtgatggaa
      601 gccctggtgc ccggcagcac cagccacatg cccgaacccg aacactggcc gcgccaggaa
      661 cgcgcgatct cgatccgcgg gctgaccgcg ggcatggcct ttcacctggg tgattgctgc
      721 cacccggtgc cgggcgaccg gatcgtcggc attcgccagc cggaaaaggg ggtggaggtt
      781 cactcgatcg attgccgcac gctggccagc ggggtcgatg ccgactggct cgacctgtcc
      841 tggggcgaac gcaccaccgg tgcggtcggg cggctgcgca tggtgcttta caaccgcccc
      901 ggtacgctgg ccgaagcggc cgggatcttc gctggcaacc gtgccaacat catcaatctt
      961 gaaaacaccg ggcgcgacga gctgttcggc acctacgaga tcgaccttga ggtcaacgac
     1021 ctggcccatc ttacccggat cgtcagcgcc ctgcgtgcca gcgatgccgt ggccgaggcc
     1081 gagcggatct gaagcctgtc agatcctccc cgaatttgct gcggaaaaat gggaaggctc
     1141 tcctacttca aacctccagc accacctcga ccaaatcccc ctcgctcact ccctcggcct
     1201 tgcgcacgct ggccttgacc ggcagcagcc agcccaggtt cttgctgggg aacagcgagg
     1261 tgttgaacgt gctgtccccg atccgcgccc tgaccttgag cgagccgaac ccgccgatgc
     1321 tcttttccag ccgccgcatc agcgcggttt ccgaaagcgc ctcgcccgct tcgccatcga
     1381 tggtcaggaa gtgccaggtg ccattgctcg atccggtcca gcgccacagc gggccggagt
     1441 gggtgatggt ttcggtcaat tgattgctct cgcaaaggcg caaaggcgca aggcagtctt
     1501 cttgagccga aggctctgta aaacctcgaa agcagtctct ggcgcatgcg ttccaattac
     1561 gagcggcttt gccgcaaata ccgtctcttc gcgcctttgc gccttggcga gagaaaccat
     1621 ctatttcgcc cgccgcaccg gcaccggcac gttgcagatg tcggccccgc ctgccgggcg
     1681 gatgaagaag gcatcgcgcc ggttggcgcg ggcatcggcg taggctgcga agctggcgct
     1741 ttcggtggag aggtattcgt aagcgggcag atcgttcacc tcggtgccga tccggatcga
     1801 ttgaatcgtc acccgctgat cgccctgggc gagatcgtag aaccccagat cgccatgccc
     1861 gcgcggcagg ctggacaagt gctcgatccc ctcgatcacc cggccgacca gcgcgatatt
     1921 gcgatcgaga tgacgcgggg catggccgat cacggtgtag agctcggccc cggtgccggt
     1981 actgggcggc atgtcgcggc ccacgccgac catgccatag cagtggacgg gccagttgtt
     2041 tgtgccatcg gtcgccaggg gccatccgtg atgaattccc gcaaacgggg cgtagggatc
     2101 cgggatgccg gcagggttgt tgcgcaagtc ctgcaaatct atcacttcac cttcaatcgt
     2161 gcgttgcagc acgtttggag ttgcagccac tttccaagct cgcgcgcgct ccatcatgat
     2221 gctgccaact tcttctgatc gaaattggta ttcactttgc ggcaccgacg ccaatcccgc
     2281 gggcagcgcc ttggccctgg ccttgtcctc cgcctccgga tcgccccact gcaccacgta
     2341 gttgtcctgg acccggttga tgctggtccc gtcccagaac cgcgccgcgg ccagcttgcg
     2401 gatgttgccg atccagcctt gcgagaatgg cgcgggcatc agctggatca ccacccggcg
     2461 cggcttgccc tttgcatcgg gggaaaggtc catcaccagc aggtccgatt gcgcgatggc
     2521 catccattcc gtcttgggcg cccgggcgac gatctcgctc ggcgcgagcg gcgatgggct
     2581 tgccttgccc gaggcgatgg cggcggaagc ctgcggcagc gcgggcgtgg cgacggcaag
     2641 cgcggctagc gcgatcaggg acttgtgcat cggcggattg tggccgggtt ggccggtgct
     2701 ttcaatcctt cgcgcga
//
LOCUS       DASUDV010000330         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1579167,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000330 DASUDV010000000
VERSION     DASUDV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579167"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFV30_07155"
     CDS             complement(<1..230)
                     /locus_tag="VFV30_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4847906.1"
                     /translation="MDQATRAEGGMIANLEGRVCVVTGAARGLGRAIAGALAAAGGRL
                     AICDLRDGELQETAAALRAQGGVVHAATCDVS"
     gene            complement(231..1097)
                     /locus_tag="VFV30_07160"
     CDS             complement(231..1097)
                     /locus_tag="VFV30_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847907.1"
                     /translation="MPARFEHAHIAVSRATLHCVSAGPADGAPLLLLHGWPQTWFMWR
                     GVIPLLAEAGYRVIAPDLTGLGDSTRPDNGYDKASIARDMAELMTALGHERFAVVAHD
                     WGGPVAFFLAAQFPQRVRAMAVFDAPVLGDGGPIEHHGRWHFGFHALPGLPEALTEGR
                     EDTYLRFFFRFAGASAEAISEEAQAEYVRAYSQPGAMTAGFNYYRAVPQDIAAVTAFR
                     ESGQRLAMPLLIYGGDPATRGRGMTALESWARVADNVTGGVAASCGHWIPEERPQWTA
                     EQVLAFLSQGVA"
     gene            complement(1104..1583)
                     /locus_tag="VFV30_07165"
     CDS             complement(1104..1583)
                     /locus_tag="VFV30_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEX4847908.1"
                     /translation="MTFFADSVGTALVDVDALPWIPFTPYSDEVFLKLIKINPINGEW
                     TTLLKLPAGMALPKHHHAGTVHVYTVAGRWRYLEHDWVAGPGSMVFETAASSHTPVAL
                     PEAEVIALNIIVGDWNLLDENDNIIAVENWRTMLKRYADYCAAAGIKPVDVTSQSGN"
     gene            complement(1626..>2109)
                     /locus_tag="VFV30_07170"
     CDS             complement(1626..>2109)
                     /locus_tag="VFV30_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX4847909.1"
                     /translation="GDTMWANMELAWERLPDHIKEEIADLRARHSMEASFMAAKPIDD
                     RLAMKARFPDPEHPVVRTHPVTGNKSLFVNGFATHFTNYHTASRVRVGQDFTNGSGEL
                     LRYLTSQAAIPEYQVRWRWTPNSVAIWDNRCTQHYAVMDYAPCVRRMNRATIIGDRPF
                     "
BASE COUNT          340 a          705 c          703 g          361 t
ORIGIN      
        1 tcactgacgt cgcaggtcgc ggcatggact acgccgcctt gcgcgcgcag cgcggcggcg
       61 gtttcctgca attccccatc gcgcaggtcg cagatggcaa ggcgtccccc ggcggcggcc
      121 agcgcaccgg cgatcgcccg ccccagcccg cgtgccgccc ccgtgacaac gcaaacccgt
      181 ccctcgaggt tggcgatcat cccgccttcg gcgcgtgttg cctgatccat tcaggccacc
      241 ccttggctga ggaaggcaag cacctgttcg gcggtccatt gcggccgctc ttccgggatc
      301 cagtgcccgc aactcgcggc caccccgccg gtcacattgt ccgccacccg cgcccagctt
      361 tccagcgcgg tcatgccgcg gccgcgcgtt gccggatcgc cgccatagat cagcagcggc
      421 atggccagcc gctggccgct ctcgcgaaag gcggtgacgg cggcgatgtc ctgcggaacg
      481 gcgcggtaat agttgaagcc ggcggtcatt gcgccaggct ggctgtaggc gcggacatat
      541 tcggcctggg cttcttccga gatcgcctcc gcgctggctc cggcaaagcg gaagaagaag
      601 cgcagatagg tatcctcgcg cccttcggtc agcgcttcgg gcaggccggg cagggcatgg
      661 aaaccgaagt gccagcgccc gtgatgttcg atcggcccgc catcgcccag caccggtgca
      721 tcgaacaccg ccatggcgcg gacccgctgc gggaactgcg cggcgaggaa gaaggcaacc
      781 ggtccgcccc agtcatgcgc caccaccgcg aaccgctcgt gccccagggc ggtcatcagt
      841 tcggccatgt cgcgggcgat cgaggccttg tcatagccgt tatccggccg cgtggaatcg
      901 cccaacccgg tcaggtccgg cgcgatcacg cggtagccgg cctcggccag cagcgggatc
      961 acgccccgcc acatgaacca ggtctgcggc cagccgtgca gcagcagcag cggcgcgccg
     1021 tccgccggtc ccgcgcttac gcaatgcagc gtcgcgcggg aaacggcgat gtgggcgtgt
     1081 tcgaaccgcg ccggcatggt ggatcagttg cccgactggc tggtgacgtc gaccggcttg
     1141 atcccggcgg cggcgcaata gtcggcatag cgcttcaaca tggtgcgcca gttctcgacc
     1201 gcgatgatat tgtcgttctc gtcgagcagg ttccagtccc cgacgatgat attgagcgcg
     1261 attacctcgg cctcgggcaa ggcgaccggg gtgtgggacg aggcggcggt ttcgaacacc
     1321 atcgagccgg ggccggccac ccagtcatgc tccaggtaac gccagcgtcc ggccacagtg
     1381 tagacgtgga ccgtgcccgc gtgatggtgc ttgggcagcg ccatgcccgc cggcagcttg
     1441 agcaacgtgg tccattcgcc gttgatcggg ttgatcttga tcagcttgag gaacacctcg
     1501 tccgaatagg gcgtaaaggg gatccacggc agggcatcga cgtcgaccag agcggtcccg
     1561 acgctgtcgg caaaaaaggt cacgggcatt ctcccggttg tgtcggtttg aattttcggt
     1621 gctggtcaga acggacggtc gccgatgatc gtggcgcggt tcatccggcg cacgcagggc
     1681 gcatagtcca tcaccgcata gtgctgggtg caacgattat cccagatcgc cacgctgttc
     1741 ggcgtccagc gccagcgcac ctggtattcg gggattgccg cctggctggt caggtaacgc
     1801 agcagttcgc ctgaaccatt ggtaaagtcc tgccccaccc gcacccggct ggcggtgtgg
     1861 taattggtga agtgcgtggc gaagccgttg acgaacagcg acttgttgcc ggtgaccgga
     1921 tgggtccgta ccaccgggtg ctccggatcg gggaagcggg ccttcatcgc cagccggtcg
     1981 tcgatcggct tggccgccat gaagctggcc tccatcgaat ggcgggcgcg cagatcggcg
     2041 atctcttcct tgatgtgatc gggcaggcgt tcccaggcca gctccatatt ggcccacatc
     2101 gtgtcgccc
//
LOCUS       DASUDV010000331         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1954447,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000331 DASUDV010000000
VERSION     DASUDV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954447"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFV30_07175"
     CDS             complement(<1..879)
                     /locus_tag="VFV30_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX4847910.1"
                     /translation="MSEQAAAAPAAHSPRMFQLDAFRGIAAFMIVFYHLTIVYRLGGP
                     FERGYLFVDLFFLLSGFVLAVSTEKKLNTGIGAFEFTRARFVRLWPLVAVGAGVAVVR
                     AFVIGMADPLTLLWWLLLDLLMIPSLAGVGPFYRYNGPQWTLFYELLANFLHALVLKW
                     VPTRWMLALAIAMGAWLVYTVDRHGSDTMGVDAPTWQTWWMALPRVGFSYVLGVWIGR
                     KYREGFRTPSLPWWLALAVPVIGITAVPYLPMGKVAGDLAFVILFLPPAMWLIAMAQP
                     PQALRPAMEWLGNFSLP"
     gene            994..1266
                     /locus_tag="VFV30_07180"
     CDS             994..1266
                     /locus_tag="VFV30_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847911.1"
                     /translation="MNRIFLAILALFAGIAAQVSPAEARVRGETEIGATLVQRATARA
                     AASVQVAAFRAEPRAEATVFEPARNQQALHGVHAPSVLLKADRARE"
     gene            1367..>2011
                     /locus_tag="VFV30_07185"
     CDS             1367..>2011
                     /locus_tag="VFV30_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4847912.1"
                     /translation="MLGAITKAIFGSSNDRYVKSLDKIVKQIAAYEPNLEALTDEELQ
                     AQTPKFRRMLEEGKTLDDLLPEAFATVREASKRVLGMRHFDVQMIGGIVLHRGEIAEM
                     RTGEGKTLVATLAVYLNALEGKGVHVVTVNDYLARRDAEWMGQIYTWLGLTVGVIVPN
                     LSEYERREAYECDITYATNNELGFDYLRDNMKHERSQMVHRPFNYAIVDEVDSIL"
BASE COUNT          397 a          687 c          639 g          288 t
ORIGIN      
        1 cggcagcgag aagttgccga gccattccat cgccgggcgc agcgcctgcg gcggctgggc
       61 catcgcgatc agccacatcg ccgggggcag gaacaggatc acgaaggcca gatcgccggc
      121 gaccttgccc atcgggagat agggcaccgc cgtgatcccg atcaccggca cggccagcgc
      181 cagccaccac ggcagcgagg gcgtgcggaa gccttcgcgg tacttgcgcc cgatccacac
      241 ccccaggaca taggaaaagc ccacccgggg cagcgccatc caccaggtct gccaggtcgg
      301 cgcgtcgacc cccatcgtgt cggagccgtg ccggtccacc gtatagacca gccaggcccc
      361 catcgcgatg gccagggcca gcatccagcg ggtcggcacc cactttagca cgagggcgtg
      421 gaggaaattg gccagcagct catagaacag cgtccactgc gggccgttgt agcgatagaa
      481 cgggccgacg ccggccaggc tggggatcat cagcagatcg agcagcagcc accacagcag
      541 ggtcagcgga tcggccatcc cgatcacgaa ggcccggacc acggccaccc cggcgcccac
      601 cgcgaccagc ggccagagcc ggacgaaccg ggcgcgggta aattcgaacg cgccgatccc
      661 ggtgttgagc ttcttctcgg tcgaaaccgc caggacaaag ccggacagca ggaagaacag
      721 gtccacgaac aggtagcccc gctcgaacgg gccgccgagc cggtagacga tcgtgaggtg
      781 gtagaacacg atcatgaagg ccgcgatgcc gcggaaggcg tccagctgga acatgcgcgg
      841 ggaatgcgcc gctggcgccg ctgccgcttg ctcgctcatc gaccggtccc ctccctgttt
      901 ggctccttac accagtccgg cgggcaaaga tcaaccgcgg caggccggga tggggtggac
      961 gcttaaccac tgcgtgacta tatgctgcag ccaatgaacc ggattttcct tgccatcctg
     1021 gccctgttcg cagggattgc cgcgcaggtt tcgcctgccg aggcgcgggt gcgcggggag
     1081 accgaaatcg gtgcaaccct ggttcagcgc gccaccgccc gggcggcggc atcggttcag
     1141 gtggcggcct tccgggccga gccgcgggcc gaagccacgg tgttcgaacc ggcccgcaac
     1201 cagcaggcct tgcacggggt tcatgcccct tcggtcctgc tcaaggctga ccgcgcgcgc
     1261 gaatagccgc cgctgacctc gttttccgcc caccgcggcg cgccggcacc cgtcagggtc
     1321 cgcgcactcc catatccata cccttcaaaa cattcaggaa ttgcccatgc tcggcgccat
     1381 taccaaggcc attttcggct cgtccaacga ccgttacgtc aagtcgctcg acaagatcgt
     1441 caagcagatc gccgcctatg aacccaacct cgaggcgctg accgacgagg aactgcaggc
     1501 ccagaccccg aagttccggc ggatgctgga agagggcaag acactggacg atctgctgcc
     1561 cgaagccttc gccacggtgc gcgaagcttc gaagcgcgtg ctggggatgc gccacttcga
     1621 cgtccagatg atcggcggca tcgtgctcca ccgcggcgag atcgcggaaa tgcgcaccgg
     1681 cgaaggcaag accctggtcg ccaccctggc ggtctatctg aacgcgctgg aaggcaaggg
     1741 cgtccacgtc gtcacggtca acgattacct ggcccgccgc gacgccgaat ggatgggcca
     1801 gatctatacc tggctgggcc tgaccgtggg cgtgatcgtg ccgaacctgt cggaatacga
     1861 acgccgcgaa gcctatgagt gcgacatcac ttacgcgacc aacaacgagc tgggcttcga
     1921 ttacctgcgc gacaacatga agcacgagcg cagccagatg gtccaccggc cgttcaacta
     1981 tgcgatcgtc gacgaagtcg actcgatcct g
//
LOCUS       DASUDV010000332         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_850281,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000332 DASUDV010000000
VERSION     DASUDV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850281"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFV30_07190"
     CDS             <1..585
                     /locus_tag="VFV30_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4847913.1"
                     /translation="RKKRWSAGIMRQAGRYGGECMAAGEAAEQTFRNAVIAALPRLRR
                     FCQGLVGNAQDGDDLMQSTVERALARSHQFVPGTRLDSWMFRIAQNLHIDGARVAGRR
                     GHQVPIDDLPETMGEDGRVTVEDRSDLVAAQRALQSIPEDQRAAFLLVVVEGLSYREA
                     AEVLEVPVGTIMSRLARARGRIEQVVGRGREALP"
     gene            582..1280
                     /locus_tag="VFV30_07195"
     CDS             582..1280
                     /locus_tag="VFV30_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847914.1"
                     /translation="MSDDIDERLAAWLDGALSTEEAAAFERELESNPELARRAADWQA
                     NDTFLKGALAPLAESPIDSDLLARMGLAEPSTMQLAANDNQPFWHRHALPLGGAVAAS
                     LAALLLLAVPQRGSEPDALSMALDTTPSLTATRLADGRTIQPTLTVRSADGRWCREFR
                     EDARTALACRGEKGWIIEGQAAASGPSATNEIGLAAGGDVAGLDAAYRRIGASDPVGQ
                     QEEMALIKQGWSRR"
     gene            complement(1308..2465)
                     /locus_tag="VFV30_07200"
     CDS             complement(1308..2465)
                     /locus_tag="VFV30_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4847915.1"
                     /translation="MKYTALFMLLLAATGTAAQADGLTADDQGLTLEEGPIELNLGGR
                     VHFDVSTFDEPAAGRTGVADADFRRARLELSGKVGEVFRFRIDREFAGRSKGWRNLWA
                     GITPIDNVELRGGNMMAPFSTEDLQSSNSIPFAERSLASALGPGYGLGGLASASGKRW
                     SAALGWFDDAIANEEGRSSERGRGVVGRLTVLPVSAGKTRLHLGLAGERRSFRPGENL
                     RLSADAGSVLAPNVMATGAITGIEHLNAWNGEAGLAQGPVLIQAQISGMDLERGALPK
                     LRFNGQTLQAAWLVTGGGYDYSRGTGNFSGPDLKRQRLALELAARYSRLDLADGTFDV
                     GTGRAVTAAANAYWGRNLRLMLDYTRTTVRFTGAVPQQTTNTGVARIQLNF"
     gene            complement(2462..3265)
                     /locus_tag="VFV30_07205"
     CDS             complement(2462..3265)
                     /locus_tag="VFV30_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847916.1"
                     /translation="MNALALVPALAAVPPRPLFLRCPGQLERDYYLLIPTNLCPEAEP
                     LVLVHGISRNAPELVLRFAELANRLGVPLIAPLFRREIYGQYQQVIDPRGGQRSDLAL
                     LDILDDAARRHGLANGRIALFGFSGGAQFAHRFAMLHADRVRVCVPSSAGWYTMPDPN
                     LAWPMGLAESPLPIHPQTQSIPFHVIVGQRERDDDEALRRNPALDAMQGENRRARART
                     WHRALRRAGWNSESSLTVLPRTRHNFSCAHRNGLVETVFRLIGHQGTEQ"
     gene            complement(3262..>4735)
                     /locus_tag="VFV30_07210"
     CDS             complement(3262..>4735)
                     /locus_tag="VFV30_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4847917.1"
                     /translation="LGATIAVARTLHGSTVVWSTAALAAMLAFGCEALLRRLSEGLGL
                     DYVTAVRDALFRHLMQVDPAIVQSRRHGAMLQSFVGDLTALRQWVAEGIMRAWLALIA
                     LTGLLGWLAWTTPTLGLTAFAITALACLAGLALLRPLARAVRTVRKERGTVAAFASER
                     LVASATVLACARTSTEARRLERRVARLNTAALRRAWLTGLLRALPHLAATAIVIAAAR
                     IAPQLPASGMAGLVLVIGIIGLALRDLARAAELAVPGRVSRHRIARMLALPVLTYPDP
                     APWQRGETRTLVLEAVRLTADQPPLSARLERGEVALLDGDPAQVGALLRILAGLAQPQ
                     AGAARWNGADLLACRPARRRRIVGLASADLPLLRGSLGMNLRYRQPDLAAGELAALAA
                     AWSLDPTSNEGSPQVTVLLRALAGKPPVLLLAPDERLLSDSDAARLADVISHWPGVVL
                     LAARHPALSRRATRHWQFGPAGLIDLPRPTPSLVSQENAA"
BASE COUNT          826 a         1532 c         1659 g          718 t
ORIGIN      
        1 agaaaaaagc gttggtctgc cggaataatg cggcaggcgg gtcgttatgg cggggaatgt
       61 atggctgccg gtgaggctgc agagcagacg ttcaggaacg cggtgatcgc cgcgctcccg
      121 cggctgcgcc ggttctgcca ggggcttgtg ggcaatgccc aggatggaga cgacctgatg
      181 caatcgacgg tggaaagggc cctggcgcgc tcgcaccagt ttgtccccgg cacccggctt
      241 gacagttgga tgttccggat cgcccagaac ctgcatatcg atggcgcgcg ggtagctggc
      301 cggcgagggc accaggtgcc gatcgacgac ttgcccgaga caatgggcga ggacggccgt
      361 gtcacggttg aagaccgttc ggatctggtc gccgcgcagc gggctttgca gtctattccc
      421 gaagaccagc gtgcagcctt cctgctggtg gtggtcgaag ggctgagcta ccgcgaggcg
      481 gccgaggtgc tggaagtgcc ggttggcacg atcatgagtc gcctggcccg cgcgcgtggg
      541 cggatcgaac aggtggtcgg acgcggccgg gaggcattgc catgagcgac gatatcgacg
      601 aacgcctcgc cgcctggctc gatggcgctc tttcgaccga ggaggccgcg gcctttgagc
      661 gagaactgga aagcaatccc gaactcgctc gccgcgctgc cgactggcaa gccaacgata
      721 ccttcctgaa gggcgcgctc gcgcccttgg ccgaatcccc gatcgacagc gatctgctgg
      781 cgcggatggg cctagccgag ccgtccacca tgcaactggc ggccaatgac aaccagccgt
      841 tctggcaccg ccatgcattg ccgcttggcg gcgcggtcgc ggcgagcctt gcggcgctct
      901 tgctgctggc cgtgccacag cgcgggtccg agcccgatgc gctcagcatg gcgctcgata
      961 ccacgccttc gctcacggct acgcgccttg ccgatggccg caccatccag ccgacgctga
     1021 cggtgcgctc ggccgatggc cgctggtgcc gggaattccg cgaggacgcc agaaccgcgc
     1081 tggcctgccg tggcgagaag ggctggatta tcgagggtca ggctgctgct tctggcccat
     1141 cggccaccaa tgagatcggc ctggcagcag gcggcgatgt ggctgggctc gatgcggcct
     1201 atcgccggat cggcgcttcg gatccggtcg gccagcagga agagatggcc ctgataaagc
     1261 agggctggag ccgccgctga cccaggccag cgacggcgcc gaccagatca gaagttgagc
     1321 tggatccggg caaccccggt attcgtggtc tgctgcggca cggcgccagt gaagcgcacc
     1381 gtcgtgcggg tataatccag catcagtcgg agattgcggc cccaataggc atttgcggcg
     1441 gccgtaacag cgcggcctgt gccgacatcg aaggtgccgt cggccagatc aagccggctg
     1501 tagcgagccg cgagttccag cgcgaggcgc tgccgcttga ggtcgggccc ggagaaattt
     1561 ccggtcccgc gcgaatagtc ataaccgccg ccggtcacca gccacgccgc ctggagggtc
     1621 tggccgttga acctgagctt cggcaaggcg ccgcgttcca ggtccatgcc gcttatctgg
     1681 gcctggatca gcaccggtcc ctgggccaga ccagcctcac cgttccacgc gttgagatgt
     1741 tcgatcccgg tgatggcacc ggtcgccatg acattgggtg ccaggacgct cccggcatcg
     1801 gctgacaggc gcagattctc gcccggacgg aaagagcggc gctccccggc cagacccaga
     1861 tggagccgtg tcttgcctgc actgaccgga aggacagtga ggcggcccac caccccgcgg
     1921 ccgcgctcac tcgaacggcc ttcctcgttg gcgattgcat cgtcgaacca gcccagtgcg
     1981 gcgctccagc gcttgccgct ggcactggcc aggccgccca gcccataacc cggcccgagc
     2041 gccgaagcga gtgagcgttc ggcgaaggga atggagttcg aactttgcag gtcctcggtc
     2101 gagaacgggg ccatcatgtt gcctccgcgc agttcgacat tgtcgatcgg ggtgatcccg
     2161 gcccagaggt tgcgccaacc cttcgaccgg cccgcgaatt cgcggtcgat cctgaagcgg
     2221 aagacttcgc ccaccttgcc cgagagttcc agtcgcgcgc ggcggaaatc ggcatcggca
     2281 accccggtac ggcccgcggc gggctcgtcg aaggtgctga catcgaagtg gacccgaccg
     2341 ccaaggttga gttcgatcgg accttcctcc agcgtcaatc cttggtcatc ggccgtgaga
     2401 ccgtctgcct gggcagcggt gccggttgct gcgagcagca gcatgaagag agcggtgtat
     2461 ttcattgttc ggttccttga tgtccaatga ggcgaaagac ggtctcgacc agcccgtttc
     2521 ggtgggcgca ggagaagttg tggcgggttc gcggcagcac ggtcaggctg gattcgctat
     2581 tccacccggc gcggcgcagc gcgcgatgcc aggtgcgcgc gcgggcgcgg cggttttcgc
     2641 cctgcatggc gtcgagtgcg ggattgcggc gcagcgcttc gtcatcgtca cgctcgcgct
     2701 gaccgacgat cacatggaac gggatcgact gcgtctgggg atggatcggt aacgggctct
     2761 cggccaatcc catcggccag gccaggttcg gatcgggcat tgtgtaccag ccggccgagg
     2821 acggaacaca gacccgcaca cggtcggcgt gtagcatggc aaaacgatgg gcgaactgcg
     2881 cgccgcccga gaagccgaac agtgcgatcc gcccgttagc aagaccatgg cggcgagcgg
     2941 catcgtcgag gatgtcgagc aaggcaagat cactgcgctg gccgccgcgc ggatcgatca
     3001 cctgctggta ctgaccataa atctcgcggc ggaaaagagg ggcaatcagc gggacgccta
     3061 gccgattggc caactcggca aaacgcagca ccagttcggg ggcattgcgc gagatgccat
     3121 ggaccagcac cagcggttct gcctcggggc acaagttggt ggggatcagc aggtagtaat
     3181 cgcgctccag ctggcccggg catcgcaaaa acagggggcg cggcggcacg gcagcaagag
     3241 cagggacaag ggcaagggcg ttcatgcggc gttctcctgg ctgaccaggc tgggggtggg
     3301 gcgcggcagg tcgatcagtc cggccggacc gaattgccag tgccgggtgg cgcggcggct
     3361 tagcgccggg tgccgcgcgg caagcagaac tacacctggc cagtgcgaaa tcacgtcggc
     3421 cagccgcgct gcatcgctgt ctgacagcag gcgctcgtca ggcgccagca aaaggaccgg
     3481 tggcttgccg gccagggcac gcaggagcac agtcacctgc ggggatcctt cgttggaagt
     3541 cggatcgagg ctccaggctg cagccagggc agccagttcg ccggctgcaa gatcgggctg
     3601 acgatagcgc aggttcatgc caagactgcc gcgcaacagc ggcaggtcgg cacttgccag
     3661 gcccacgatc cggcggcgcc gggccggacg gcacgccagc aggtcggcgc cgttccagcg
     3721 ggccgctcca gcttgcggct gggccaggcc cgccaggatc cgcagcagcg ctcccacctg
     3781 tgccggatcg ccatcgagca aggccacttc gccccgctca agccgcgcgg agagcggcgg
     3841 ctggtcggca gtcaaccgga ccgcctccag caccagcgtg cgggtctcgc cgcgctgcca
     3901 gggggcggga tcgggatagg tcagcaccgg cagggccagc atcctggcaa tccggtggcg
     3961 cgatacgcgg cccggcactg ccagttcggc tgcccgcgcc agatcgcgca gcgcgagacc
     4021 gatgatcccg atcaccagca ccagtccagc cattccactg gctggaagtt gcggtgcgat
     4081 ccgggcggca gcgatgacta tggcggttgc ggcaaggtgt ggcagggcac ggagcagccc
     4141 ggtcagccag gcccggcgca atgcggcggt gttgagccgg gccacccggc gctcaagtcg
     4201 acgcgcctcg gtgcttgtcc gggcacaggc aagtaccgtc gcgctggcga caagccgttc
     4261 agaagcaaag gccgcgactg tgccacgctc cttgcgcaca gtccgcacgg ctcgcgccag
     4321 gggacgcagc agcgccagtc cggcgaggca ggcaagagcg gtgattgcga acgcagtcag
     4381 gcccagcgtc ggtgtggtcc aggccagcca gcccagcagg ccggtcagcg cgatcagcgc
     4441 cagccaggcg cgcatgatgc cttcggctac ccactggcgc agtgcggtca ggtcgccgac
     4501 aaagctttgc agcatggcgc catggcggcg gctctgcact atcgccggat cgacctgcat
     4561 caggtgccgg aacagtgcat cgcgcaccgc ggtgacatag tcgagcccca acccttcgct
     4621 gagccggcgc agaagggcct cgcagccgaa agcaagcatg gcggcaaggg ctgcggtcga
     4681 ccagaccacg gtcgacccat gcagcgtccg cgccacggca atcgttgcac caagc
//
LOCUS       DASUDV010000333         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1623444,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000333 DASUDV010000000
VERSION     DASUDV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623444"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFV30_07215"
     CDS             complement(<1..915)
                     /locus_tag="VFV30_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX4847918.1"
                     /translation="MTRIRPFTLAIPQDQIDDLNARLDRTRWPEKETVTDWSQGTPLG
                     VLQELVGYWRRGYDWRACEARLNALGQFTTEIDGLDVHFIHHRSPHADALPLVMTHGW
                     PGSVVEFLGVIEALANPQDGSLPFHVVAPSLPGFGFSGKPATTGWGVEKIGRAWGELM
                     ARLGYTRWVAQGGDWGSAVTTAIALQQIEGCLGIHLNMPIARPGPDDLAAPGPAELKA
                     LGALQHYQEWDSGYSKQQATRPQSVGYALVDSPVGLAGWIVEKMWAWTDNSGSPYDAL
                     SRDQILDNLMLYWLPASGASSARLYWESF"
     gene            1043..1879
                     /locus_tag="VFV30_07220"
     CDS             1043..1879
                     /locus_tag="VFV30_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX4847919.1"
                     /translation="MNQSGHKARFGTGESYLMRHLYYLTLPLAMTAGTAWAEDLPANT
                     DGASDTEIEIATGADYSVGDYGAADDTRVWSIPLDLKVRSGRVRLQASLPYVFVNGPG
                     QVVGGVIVPTPGGVTTSRSGIGDLNLSGGYTLVNEDGGVPMIELGGAVKLPTAKTTIG
                     TGKADFTASASVYKSLGSTVMLFGTVGYSWLGSPAQYALKNGVMASGGLNLRPDDSQN
                     YGVSLAWREPVAAGYAGQAVVSPYMTYRINKRFGITLYGMAGLNDASPRAGGGLRLSL
                     FQ"
     gene            1926..2795
                     /locus_tag="VFV30_07225"
     CDS             1926..2795
                     /locus_tag="VFV30_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847920.1"
                     /translation="MKSRISRSMGLACLALLGASAAIAGPPGGVPVGPPPGIPHGPPP
                     GVGHGPPAGIPSGPPAGIPKGPPAAATAHIPAAASARMPQGQARAQGAASHSQAQTGL
                     AHAPKDVQKGASLLKKLNAAHASDKAFEHASDKSIVGAIGAYKEDTKQALADIETYTD
                     KVATGTAAVATAEGAVSDAQGAVTTAQASVDAAQAKLDAAKAASPVDQAAIDAAQTEL
                     NTANTNLATAKTNLETANTNLATAKDALAADQAKLAEAQTLLTSAQTTLADKSGVELT
                     PEVIEKLNDLLNL"
     gene            complement(2806..>3160)
                     /locus_tag="VFV30_07230"
     CDS             complement(2806..>3160)
                     /locus_tag="VFV30_07230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4847921.1"
                     /translation="AVLEYEAGAHLCHSGGVVQGGFVTGWIDAAMAHAAIARHGQDVT
                     PMTLEMKISFFAPTRPGRVFAEGWVERDGRRTTFYEGCLKDSAGNVLAKGSSTILLAD
                     RSCVEAAARAALDAG"
BASE COUNT          579 a         1077 c         1008 g          496 t
ORIGIN      
        1 gaagctttcc caatagagcc gcgccgagga cgcgccgctg gccgggagcc agtagagcat
       61 caggttgtcg aggatctgat cgcggctgag cgcatcgtag ggcgagccgg aattgtcggt
      121 ccaggcccac atcttctcaa cgatccaccc ggccagcccc accgggctat cgaccagggc
      181 gtagccgacc gattgcgggc gtgttgcctg ctgcttcgaa tagcccgaat cccattcctg
      241 gtagtgctga agcgcgccca gcgccttcag ctcggccggg ccgggcgcgg ccagatcatc
      301 gggcccgggc cgggcgatcg gcatgttgag gtgaatgcca aggcaacctt cgatctgctg
      361 cagcgcaatc gcggtggtca ccgccgagcc ccagtctccg ccctgggcaa cccaccgggt
      421 atagcccagc cgcgccatca gttcgcccca ggcgcggccg atcttctcca ccccccaacc
      481 ggtggtggcc ggcttgccgg aaaagccgaa acccggcagc gacggggcga ccacgtggaa
      541 cggcagcgaa ccgtcctgcg gattggccag cgcctcgatc acccccagga actccaccac
      601 cgatccgggc cagccgtggg tcatcaccaa cggcagcgca tcggcatggg gcgaacggtg
      661 gtggatgaag tgcacatcca gcccatcgat ttcggtggtg aactggccga gcgcgttcag
      721 ccgtgcctcg caggctcgcc aatcgtagcc gcggcgccaa tagcccacca gttcctgcag
      781 cacgcccagc ggcgtgccct gcgaccagtc cgtcaccgtt tccttttcgg gccagcgcgt
      841 ccgatcgagt cgggcgttga gatcgtcgat ctggtcctgg ggaatggcga gcgtgaacgg
      901 gcgaatccgg gtcatgccag caccctatgc catttcgcgg cgggtgcaac gcgtgccttg
      961 ccagcggttc gcgtcagctt ggcaccggcc cgccgcaggt gagcttggtt aacccgcgct
     1021 ttcccacata aggcaaggcc aaatgaacca atcgggccac aaggctcggt tcggaaccgg
     1081 agaaagctat ctgatgcggc acttgtacta cctcaccctc cccctggcca tgacggcggg
     1141 aaccgcctgg gcggaagacc tgcctgccaa cacggacggt gcttcggata ccgagatcga
     1201 aattgccacc ggcgccgact attcggtggg cgattacggc gcggccgacg atacccgggt
     1261 atggagcatt ccgcttgacc tcaaggtgcg ttcggggcgg gtccggctgc aggccagcct
     1321 gccctatgtc ttcgtcaatg gtccgggcca ggttgtaggc ggggtgatcg tgccgacacc
     1381 gggcggggtg accacttcgc gttcggggat tggcgatctc aacctttcag gcggctacac
     1441 gctggtaaac gaagatggcg gcgtgccgat gatcgaactg ggcggcgcgg taaagctgcc
     1501 caccgccaag accacgatcg gcaccggcaa ggccgatttc accgccagcg cctcggtcta
     1561 caagtcgctc ggatcgaccg tgatgctgtt cggcacggtg ggctattcgt ggctgggcag
     1621 cccggcgcaa tatgcgctga agaacggggt gatggcatcg ggcgggctca acctgcgccc
     1681 ggatgacagc cagaactatg gcgtgagcct ggcctggcgt gaaccggttg ccgcaggtta
     1741 tgccgggcag gctgtggtct cgccctacat gacctaccgg atcaacaagc gcttcgggat
     1801 cacgctctat ggcatggccg ggctcaacga tgccagcccg cgggctggtg gcgggctgcg
     1861 gctaagcctg tttcaatgat cgcaacaggt ctgaattttc gggggccaat gaaggagaag
     1921 tcgcaatgaa gtcgcgaatt tcacgcagta tgggcctcgc ctgtctggcc ctgcttggcg
     1981 ccagcgccgc gatcgccggc ccgccgggag gtgtgccggt tggtccgccg ccgggcattc
     2041 cgcacggccc gcccccgggg gttggccatg gcccgccagc cggaatcccc tcgggcccac
     2101 cggccggtat ccccaaggga ccgcccgctg ccgccacggc tcacatccct gccgccgcat
     2161 cggcccgcat gccgcaaggc caagccaggg cccagggcgc ggcaagccat tcgcaggccc
     2221 aaaccggcct ggcccatgcg cccaaggatg tgcagaaagg cgcttcgctg ctcaagaagc
     2281 tgaacgcggc gcatgcctcg gacaaggcgt ttgagcatgc ttcggacaag tcgatcgtcg
     2341 gtgcgatcgg ggcgtacaag gaagatacca agcaggcgct ggccgatatc gaaacctata
     2401 ccgacaaggt tgcgactgga accgctgccg tggccactgc cgagggtgca gtaagcgatg
     2461 cccagggcgc ggtaaccacg gcccaggctt cggtcgacgc cgcccaggca aagcttgacg
     2521 cggcaaaggc ggccagcccg gtcgatcagg ccgcgatcga tgccgcccag accgaactga
     2581 acacggcgaa caccaacctc gccacggcga agaccaacct ggaaaccgcc aacaccaacc
     2641 ttgccaccgc caaggatgcc ctggcggcgg atcaggccaa gctggccgag gcgcagaccc
     2701 tgctgacttc ggcccagacc acgctggccg acaagtcggg cgtcgaactg acccccgagg
     2761 tcatcgaaaa gctgaacgac ctgctcaacc tctgagcttc ccggctcaac ccgcgtcgag
     2821 cgctgccctt gcggcggcct cgacgcagga tctatcggcc agcaggatgg tcgaactgcc
     2881 cttggccagc acattgccgg cactgtcctt caggcaccct tcgtagaatg tggtccgccg
     2941 cccatcgcgt tcaacccaac cttcggcaaa gacccggccc ggacgggtcg gggcaaagaa
     3001 actgatcttc atttccagcg tcatcggggt gacatcctgg ccatggcgcg caatcgcggc
     3061 atgggccatc gccgcatcga tccagccggt cacgaagccg ccctgcacca ccccgcccga
     3121 atggcacagg tgcgcacccg cttcgtattc cagcaccgcc
//
LOCUS       DASUDV010000334        12759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1998991,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000334 DASUDV010000000
VERSION     DASUDV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998991"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="VFV30_07235"
     CDS             complement(<1..1529)
                     /locus_tag="VFV30_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEX4847922.1"
                     /translation="MTLSAAAWAGIDTVLWSAGALACLIGAAWLMTRQSRFAAARPAL
                     VFALLASAGWAIAGAVFGVGTGLVAIGEGLRNLAWIYAVYRLFAIDGRHASIKPVRPL
                     LATLAGVVLLQMAGDLLVMARDANLFGQIGLRANVLLHLLIAIGGLVLAHNLYSGASQ
                     AGRQILRWPAATLAMLWTVDLNHYAIAYLSGANPEVTGALRGLLGVPMAGLLVLGAGE
                     RSSDLRFMPSRVVAFQSISLLLIGGYLAALVAAAQWLAFAGSDYSSLLQIAFVAVAAV
                     TVLALSGSARLRGWLRVTVLKHLFQHRYDYRVEWLRLTRTMGQSDADGLALDQRIIRG
                     LADLTDSPAGQLLTPSEAGDFELAARWQWPTAEVPAGGFPARLAQSIAQGGYIAEIDA
                     LRREPNPVHRLPDWLMADPRAWVVVPLLHFERMVGMVVLARPPHARQLDWEDFDLLRV
                     VGRQLATYLAEQAGQAALVEAARFDEFNRRIAFVMHDIKNLASQMALLARNAESHADN
                     P"
     gene            complement(1531..2916)
                     /locus_tag="VFV30_07240"
     CDS             complement(1531..2916)
                     /locus_tag="VFV30_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEX4847923.1"
                     /translation="MIRLFKHYVPHAVLLLGLIDVALLFFVADFGWRFRESQIGIESE
                     ALASRFWQLTGYAGIMLMAMIAVGVYGAEALRSIRFATARLLVAISLGIIGLVFVDFL
                     FGGHNFWRSVLAYAMAGSIIVLVANRLVVGGVLGAAAFRRRVLVLGAGQRADRLRKLS
                     ERPESGFVIVGYVGMTESEPVIAEAINRGAIDNLTRHVENLNVSEVVLALEERRNSLP
                     LADLLRIKTTGVHVNDFSSFIERETGRVDLDTVNPSWLIFSDGFSSGRAISSAMKRLF
                     DIAASSLLLILSAPIIALFALLVKIDSKGPAFYRQQRVGLYGQPFDVIKLRSMRTDAE
                     VNGAQFAQENDPRVTRLGRFIRKVRIDELPQTWSVLKGEMSFVGPRPERPQFVSELEE
                     QLPYYAERHMVKPGITGWAQINYPYGASLEDSRHKLEFDLYYAKNYTPFLDLLILLQT
                     LRVVLWPEGAR"
     gene            complement(3010..4398)
                     /locus_tag="VFV30_07245"
     CDS             complement(3010..4398)
                     /locus_tag="VFV30_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX4847924.1"
                     /translation="MVDSAFLAGPAIRRLRRREGLTQAAMAARLAISPSYLNLIERNQ
                     RPLSARLLVQLAEAFDFDPRSLRQDEAVGGVDGLRRRLADERFADLGIDRDEIAEWLA
                     AAPQAALAFARLYDAGGGAGAAAVSDPMDLARREIERWRNHFADLDVAAEELADELRL
                     SNAEMGAALAERLRQKHQLSVRILPYDVMPDALRRLDLHARQLQLSELLDQSSRNFQV
                     ALQLAMLEQGEAIAAIADGAQFADRAAWRLFRRHLAGYFAAALLMPYGRFMRACEATG
                     YDLTVLQRRFSVGFEQLAHRLTTLQRVGQRGLPFFMARIDRAGQFSKRYAGASGTTML
                     ESDVACPLWNVHRAFEQAGRIQVQAVDFAETSGQTSHWFTMARTVEGSAAPGGVPAQF
                     AVVLGLEAGLANQLAQARDLSLDPARAVPIGPGCARCHRSGCQQRSLPPRGAALQFDE
                     RSRGLTPFAFGR"
     gene            4544..6130
                     /locus_tag="VFV30_07250"
     CDS             4544..6130
                     /locus_tag="VFV30_07250"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX4847925.1"
                     /translation="MTYTDNITQADQTIAKNANWDGISAESVARMRLQNRFKTGLDIA
                     KYTAKIMRDDMAAYDADPAAYTQSLGCWHGFIGQQKLISIKKHFGTTKGRYLYLSGWM
                     IAALRSEFGPLPDQSMHEKTSVPALIEELYTFLRQADARELGGLFRELDEARKAGDEV
                     KAKAVQAKIDGFETHVVPIIADIDAGFGNAEATYLLAKKMIEAGACALQIENQVSDEK
                     QCGHQDGKVTVPHEDFLAKIRAIRYAFMELGVDDGIIVARTDSLGAGLTKQIAFTREA
                     GDIGDQYNSFLDCEEVTEIGHGDVLISRDGKLLKPKRLPSNLYQFRAGTGEDRCVLDC
                     ITSLQNGADLLWIETEKPHIGQIGGMVSRIREVIPNAKLVYNNSPSFNWTLNFRQQVF
                     DAWTEAGKDVSAYDRAKLMSVDYDTSELAIEADERIRTFQRDAAAQAGIFHHLITLPT
                     YHTAALSTDNLAKEYFGEAGMLGYVKGVQRQEIRQGIACVKHQNMSGSDIGDDHKEYF
                     AGEAALKAAGEHNTMNQFAA"
     gene            6290..6847
                     /locus_tag="VFV30_07255"
     CDS             6290..6847
                     /locus_tag="VFV30_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847926.1"
                     /translation="MRRQAPPLPISPKQLKHFAIATVVLTGLLALLASGTGSGVAAEI
                     DARKAKNDLVAAEQEKVGTKKVGTAIKVRDKSMNSTFADDSSLGDGGFGGSSSGGNSY
                     AGPRAGPRPMPALQSPIGLQNRPGGTVTRKRMGKPPPKPGELDDPDQAGEDGTGGAPR
                     PDMSRLEEVKQASRQRSGSANADGD"
     gene            complement(6844..7440)
                     /locus_tag="VFV30_07260"
     CDS             complement(6844..7440)
                     /locus_tag="VFV30_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX4847927.1"
                     /translation="MPRDTRTITRDDILPLDQYELIRADKKQEALARKALTRIAVGPH
                     ATVLFENWDSMWLQVQEMLRIEKGGDEQLSDELAAYDPMVPKGSELTCTLLFEIGDPV
                     RRDAFLRTIGGVENHVAIQVGDFLIRARPEGDTERTRESDNKASAVHFFHFDFPAAAI
                     AAWKSGEGNAMLVIDHPNYGHAALIGADSRAFLARECF"
     gene            complement(7450..8775)
                     /locus_tag="VFV30_07265"
     CDS             complement(7450..8775)
                     /locus_tag="VFV30_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX4847928.1"
                     /translation="MEGSLEAPTRHPIPWQDESWYDEAALEAELRRVYDICHGCRRCF
                     NLCDSFPILFDAIDEAPNEEVEDLTPVQLAQVVDACTLCDMCFMTKCPYVPPHSFDLD
                     FPHLMLRHRAVQHRKGGTSLADKQLAEMDRNGRLGTILSGAVNWATKEDNSLTRPVIE
                     KVTGIDRRAALPPFMDVSLTRQAVAIVPQPNPDGPAFGKKVAIYAGCHDEFNDGGPGL
                     AMVKVLAHNGVQVRIEHPDCCAMPKFENGDLPAVASAATRISAHFAPLIEQGWDVVAL
                     TTSCALMLKFEWPLIEPENETVKLLSRHTFDVAEYIVGLSKDTGLAPIEPMDKRIAVH
                     FACHARAQNMGPKAMEMLRLIPEAKPALTERCSGHGGKWGIFKENFDRAVKVGKPAAR
                     NLMKDQPDLVVSECPLAGPHLKQVIAANGAEPPARIGHPIEVLAQAYGL"
     gene            complement(8879..9304)
                     /locus_tag="VFV30_07270"
     CDS             complement(8879..9304)
                     /locus_tag="VFV30_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX4847929.1"
                     /translation="MGLKGSKTEENLKAAFAGESQANRRYLYFAQKADIEGHNDVAAV
                     FRSTAEGETGHAHGHLEYLEECGDPATGEPIGDTVANLKASIAGETHEYTDMYPGMAK
                     TARDEGFDEIADWFETLAKAEKSHAGKFQKTLDGLLADA"
     gene            complement(9443..9526)
                     /locus_tag="VFV30_07275"
     tRNA            complement(9443..9526)
                     /locus_tag="VFV30_07275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9491..9493),aa:Leu,seq:caa)
     gene            complement(9597..10421)
                     /locus_tag="VFV30_07280"
     CDS             complement(9597..10421)
                     /locus_tag="VFV30_07280"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4847930.1"
                     /translation="MAVVSGIIRVMEKAARKAGGRLRRDFGEIEHLQVSRKGPADFVS
                     KADQRAERTIWDELRAARPDWGFLMEEGGEIHGAEGKPRFIVDPLDGTSNFLHGIPHF
                     AISIAVQEPKLDGSGWGDVTAALVYQPITDESYWAEKSRGAWLHDGRLRVSARRHLDE
                     SLIATGIPFAGAGEPAEWMKIYSALAPQVAGIRRFGAASLDLAWVAAGRYEGFWESGL
                     SPWDTAAGCLLVREAGGFVSDWRGISQPVCDKQVLAGNDALHSRLHKVIVGALKAG"
     gene            complement(10424..10795)
                     /locus_tag="VFV30_07285"
     CDS             complement(10424..10795)
                     /locus_tag="VFV30_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847931.1"
                     /translation="MAKAATSLVGLNLQDSWLKGLLMEGSSLKLRVDLHITQDHPGHR
                     PAKEGEWACYRSGWLEFRNCRELSLEQTGKTLPRSLDCYDFGTFDHVAIRCDHFEIET
                     EIFKLNFQADGVAVSLDEERK"
     gene            complement(10802..11368)
                     /gene="efp"
                     /locus_tag="VFV30_07290"
     CDS             complement(10802..11368)
                     /gene="efp"
                     /locus_tag="VFV30_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX4847932.1"
                     /translation="MAKISGVDIRPGNIIEYEKGIWKVAKTQHTQPGKGGAFMQVEMK
                     NLMDGRKTNVRFRSADTVEKVRLDTKDFQYLYAEGDDLVFMDKDNFEQITLPRDLLGD
                     AADFLQDGMDVVLELWEERPVSVELPQHIEATIVEADAVVKGQTASSSYKPAILDNGV
                     RVMVPPHISSGTRIVVDVYERSYVGKAD"
     gene            11492..11923
                     /locus_tag="VFV30_07295"
     CDS             11492..11923
                     /locus_tag="VFV30_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847933.1"
                     /translation="MLRYALPAALLAASAAALAVPGGPIGVLAPASYACEMPGDAVSA
                     AGYRVDAENFTIVNSNTYYTASGRGTYLLTGDLLVLTSGPKRGQKFRKISANFLRKLG
                     PDGSETALRCVRRVVNNSNGSGCDSSAAGSTPGASCPAPRR"
     gene            complement(11925..>12759)
                     /locus_tag="VFV30_07300"
     CDS             complement(11925..>12759)
                     /locus_tag="VFV30_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX4847934.1"
                     /translation="LIGASNSPISGVGILASLGISLLLVAIFGQGGDEASTRALVAFA
                     LFVTAIVFGIATISNDNLQDLKTGQLVGATPWRQQVALILGVIFGAIVIPPILDLLNT
                     TFGFQGAPGAGADALAAPQAALITAIAQGVLGGNLDWGLIGLGAGIGAGVILVDEALR
                     KTGKGSLPPLAVGMGMYLPMSLTVLIPLGAYLGHRYNRWAERQAEPAFAERMGVLAAT
                     GLIVGESLFGVLFAGIAAATGSPEALAVVESGAWTPLLGALVFAGAVTWLYRWTRRTA
                     A"
BASE COUNT         2280 a         4353 c         4087 g         2039 t
ORIGIN      
        1 tccggattgt cggcatggct ttcggcgttg cgcgccagca gcgccatctg gctcgccagg
       61 ttcttgatgt cgtgcatcac gaaggcgatc cggcggttga attcatcgaa ccgggccgct
      121 tcgaccagcg ccgcctgccc ggcctgttcg gccagatagg tcgcgagttg gcggcccacg
      181 acacgcagca ggtcaaaatc ttcccaatcg agctggcggg catggggcgg acgggccagc
      241 acgaccatgc cgaccatccg ttcaaagtgc agcagcggca ccaccaccca ggcccgcgga
      301 tcggccatca gccaatcggg cagacggtgc accggattgg gttcgcgccg cagcgcgtcg
      361 atttcggcga tatagccgcc ctgcgcgatc gattgggcca gccgggccgg aaagccgcca
      421 gccggaacct cggccgtggg ccactgccac cgcgccgcca gttcgaaatc gcccgcttcg
      481 gacggagtca gcagctggcc cgccgggctg tcggtcaggt cggccaggcc gcggatgatc
      541 cgctggtcca gcgccagccc gtcagcatcg gactggccca tggtccgggt cagccgcagc
      601 cactcgacgc ggtagtcata gcggtgctgg aacaagtgct tcagcacggt cacgcgcagc
      661 cagccgcgca gccgggccga tcccgacagg gccagaaccg tgaccgccgc caccgcgacg
      721 aaagcaatct gcagcagcga ggaatagtcg ctgccggcaa aggccagcca ctgcgccgcg
      781 gccaccagcg cggccagata accgccgatc agcagcagcg agatcgactg gaacgccacc
      841 acccgcgagg gcatgaagcg caggtcgctg ctgcgttccc ccgcgcccag caccagcagg
      901 ccggccatcg gcacgcccag caacccgcgc agcgccccgg tcacttccgg attggcgccc
      961 gagagatagg cgatcgcata gtggttgaga tcgactgtcc acagcatcgc cagcgtggcc
     1021 gccggccagc gcaggatctg ccgcccggcc tgggaagcgc cgctgtagag gttgtgcgcc
     1081 agcaccagcc cgccaatcgc gatcagcagg tgcagcagga cattggcgcg cagcccgatc
     1141 tgcccgaaca ggttggcatc gcgggccatg accagcaggt cgcccgccat ctgcagcagg
     1201 accacgccgg ccagcgtggc cagcagcggc cgcaccggct tgatcgaggc gtgccgcccg
     1261 tcgatcgcga acagccggta gacggcatag atccaggcca ggttgcgcag gccttcgccg
     1321 atggcgacca gacccgtgcc gaccccgaac accgccccgg caatcgccca gccggcgctg
     1381 gccagcagcg caaagaccag ggccggccgc gctgccgcaa agcggctttg ccgggtcatc
     1441 agccaggccg cgccgatcag gcacgccaga gcccccgcac tccacagcac ggtgtcgatc
     1501 ccggcccagg ctgcggcgga aagggtcacg tcagcgcgcc ccctccggcc agagcaccac
     1561 gcgcagggtc tgcagcagga tcagcaggtc caggaacggg gtgtagttct tcgcgtaata
     1621 gagatcgaat tccagcttgt gccggctgtc ttccaggctg gcaccatagg gatagttgat
     1681 ctgcgcccag ccggtgatgc cgggcttcac catgtggcgc tcggcgtaat agggcagctg
     1741 ctcttccagt tcggagacga actgcggccg ttccgggcgc gggccgacaa agctcatctc
     1801 gcccttcagg accgaccagg tctgcggcag ctcgtcgatc cgcaccttgc ggatgaaccg
     1861 gccgaggcgg gtgacgcgcg gatcgttttc ctgcgcgaac tgcgccccgt tcacttcggc
     1921 atcggtgcgc atcgagcgca gcttgatcac atcgaacggc tggccataaa gcccgacgcg
     1981 ctgctggcgg taaaaggccg gccccttgct gtcgatcttc acgagcaacg cgaacagcgc
     2041 gatgatcggc gcggacagga tcagcagcag cgagctggcg gcaatatcga acagccgctt
     2101 catcgcgctg gaaatcgccc gtccggagga aaacccgtcg gagaagatca gccagctggg
     2161 attgaccgtg tcgagatcga cccggccggt ctcccgctcg atgaagctgg agaagtcgtt
     2221 gacgtggacc ccggtggtct tgatccgcag caggtcggcc agggggagcg aattgcgccg
     2281 ctcttccagc gccagcacca cttcgctgac gttgaggttc tcgacatggc gggtcaggtt
     2341 gtcgatcgcg ccgcggttga tcgcttcggc aatcaccggc tcgctttcgg tcatgccgac
     2401 atagccgacg atcacgaagc cgctttcggg ccgttccgac agcttgcgca gacggtccgc
     2461 gcgctggccg gcgccgagca ccagcacccg ccggcgaaag gccgccgcgc ccagaacgcc
     2521 gcccacgacc aggcggttgg ccaccagcac gatgatcgaa ccggccatcg cgtaggccag
     2581 caccgagcgc cagaaattgt gtccgccgaa caggaagtcg acaaaaacca ggccgatgat
     2641 ccccagactg atcgccacca gcagccgggc cgtggcaaag cggatcgagc gcagcgcctc
     2701 ggccccgtag acgccgaccg cgatcatcgc catcagcatg atgccggcat aaccggtcag
     2761 ctgccagaac cgcgaggcga gcgcctcgct ttcaatcccg atctgcgatt cgcggaaccg
     2821 ccagccgaaa tccgccacga aaaacagcag cgcaacgtcg atcaggccga gcagcagcac
     2881 ggcgtgggga acatagtgtt tgaacagccg gatcatggcc agcgacagcc ctcttctgct
     2941 acctggcagc cgtgctaccg ggcagaagtg aaatgtcggt aaactttaca gaacttggtt
     3001 aagccgcgtt catcttccga aagcgaaggg ggtcaggccg cggctgcgtt cgtcgaactg
     3061 cagcgccgcc ccgcgcggag gcagcgagcg ttgctggcag ccgctgcggt ggcaacgcgc
     3121 gcagcccggc ccgatcggca ccgcgcgggc cggatcgagc gacaagtccc gcgcctgggc
     3181 cagctggttt gccaggcccg cttccagccc cagcaccacg gcgaactgcg ccggaacgcc
     3241 gcccggcgcg gcgctgccct ccaccgtgcg ggccatggtg aaccagtgcg aggtctggcc
     3301 cgaagtctcc gcgaagtcga ccgcctggac ctggatccgc ccggcctgct cgaacgcccg
     3361 gtgaacgttc cacagcggac aggccacgtc gctttccagc atggtcgtgc cgctggcccc
     3421 ggcatagcgc ttggaaaact gcccggcgcg gtcgatccgg gccatgaaga acggcaggcc
     3481 gcgctggcca acccgctgca aggtggtgag gcggtgggcg agctgttcga accccacgct
     3541 gaagcggcgc tgcagcacgg tcaggtcata gccggtcgcc tcgcaggcac gcatgaaccg
     3601 gccgtaaggc atcagcagcg ccgccgcgaa gtatccggcc aggtggcggc ggaacaggcg
     3661 ccaggccgcc cggtcggcaa attgggcgcc gtcggcaatc gccgcgatcg cctccccctg
     3721 ttccaacatg gcgagttgca gcgcgacctg gaaattgcgc gaggattggt cgagcagttc
     3781 cgacagttgc agctggcggg cgtggagatc gagccgccgc agcgcatcgg gcatcacgtc
     3841 atagggcagg atccgcaccg agagctggtg cttttgccgc agccgctcgg ccagggccgc
     3901 gcccatttcg gcgttggaga gccgcaattc gtcggccagt tcctcggccg cgacatcgag
     3961 atcggcgaag tggttgcgcc agcgctcgat ctcgcgccgc gccaggtcca tcggatcgct
     4021 caccgccgcg gcccccgccc cgcccccggc atcgtagagc cgggcaaagg ccagcgcggc
     4081 ctggggcgcg gcggccagcc attcggcaat ctcgtcacgg tcgatcccga gatcggcaaa
     4141 gcgttcgtcc gccagccgcc ggcgcaggcc gtcaaccccg ccgaccgcct cgtcctggcg
     4201 caggctgcgc ggatcgaaat cgaaggcttc ggccagctgg accagcagcc gcgcggacag
     4261 cgggcgctgg ttgcgttcga tcaggttgag gtagctgggg gaaatcgcca gccgcgcggc
     4321 catggccgcc tgggtcagcc cttcgcggcg gcgcagacgg cggattgcgg gtccggccag
     4381 gaatgcggaa tcgaccatcg cggcgttttg taattatctt tacagatttg caagcataaa
     4441 ccgccccttg tccgcataga gacaagaatt gttgtaattt ctcgtacggg aatcagcctg
     4501 tgccggttag gtctggggca acccctccca ttggagacat tccgtgactt acaccgacaa
     4561 catcacccag gccgaccaga ccattgccaa gaacgccaac tgggatggga tcagcgccga
     4621 atccgtcgcc cgcatgcggc tgcagaaccg cttcaagacc gggctcgaca tcgccaagta
     4681 caccgccaag atcatgcgcg acgacatggc cgcctatgat gccgatccgg ccgcctacac
     4741 ccagtcgctg ggctgctggc acgggttcat cgggcagcag aagctcatct cgatcaagaa
     4801 gcacttcggc accaccaagg gccgttacct ctacctctcg ggctggatga tcgccgcgct
     4861 gcgcagcgag ttcggccccc tgcccgacca gtcgatgcac gagaagacca gcgttccggc
     4921 gctgatcgag gaactctaca ccttcctgcg ccaggccgac gcccgcgaac tgggcggcct
     4981 gttccgcgag ctcgacgaag cccgcaaggc cggagacgag gtcaaggcca aggccgtcca
     5041 ggccaagatc gacggcttcg aaacccacgt cgtgccgatc atcgccgaca tcgacgccgg
     5101 gttcggcaac gccgaggcga cctaccttct cgccaagaag atgatcgaag ccggcgcctg
     5161 cgcgctgcag atcgaaaacc aggtgtccga cgaaaagcag tgcggccacc aggacggcaa
     5221 ggtcaccgtt ccgcacgaag acttcctcgc gaagatccgc gcgatccgtt acgccttcat
     5281 ggaactgggc gtcgacgacg ggatcatcgt ggcccgcacc gactcgctgg gcgccggcct
     5341 caccaagcag atcgccttta cccgcgaagc cggcgacatc ggcgatcagt acaacagctt
     5401 cctcgattgc gaggaagtga ccgaaatcgg ccacggcgac gtcctgatca gccgcgacgg
     5461 caagctgctg aagcccaagc gcctgcccag caacctctac cagttccgcg ccggaacagg
     5521 tgaagaccgc tgcgttctgg actgcatcac cagcctgcag aacggcgccg acctgctgtg
     5581 gatcgaaacc gaaaagcccc acatcggcca gatcggcggg atggtcagcc ggatccgcga
     5641 agtcattccc aacgccaagc tggtctacaa caactcgccc agcttcaact ggacgctgaa
     5701 cttccgccag caggtgttcg atgcctggac agaagcgggc aaggatgtct cggcctatga
     5761 ccgcgccaag ctgatgagcg tcgactacga caccagcgag ctggcgatcg aagccgacga
     5821 gcggatccgc accttccagc gcgatgccgc ggcgcaggcc gggatcttcc accacctgat
     5881 caccctgccg acctaccaca ccgcggcgct cagcaccgac aacctggcca aggaatactt
     5941 cggcgaggcg ggcatgctgg gttacgtcaa gggcgtgcag cgtcaggaaa tccgccaggg
     6001 catcgcctgc gtgaagcacc agaacatgtc tggcagcgac attggcgacg accacaagga
     6061 atacttcgcc ggcgaagcgg ccctgaaggc tgccggcgaa cacaacacga tgaaccagtt
     6121 cgcggcgtaa cgccggactg acacccgttc gcggcccctg ccgcaccgat ctcctgggga
     6181 ggagaaacga tggccgtcgg agtgcccctt tcactccggc ggccatcaag ttttccttaa
     6241 ccacgatccc gaaccgggcg gttaatcttc gtgcgctagg cccacgccca tgcgacgcca
     6301 ggccccaccc ctgccaattt caccaaagca gctgaagcac ttcgcgattg cgaccgtggt
     6361 gctgaccggc ttgctggcgc tgcttgccag cggcaccgga tcgggcgtcg cggcggaaat
     6421 cgacgcgcgc aaggccaaga acgatctggt cgcggccgag caggaaaagg tcggcaccaa
     6481 gaaggtcggc accgcgatca aggtgcgcga caagtcgatg aattcgacct ttgccgatga
     6541 cagctcgctg ggcgacggcg gctttggcgg aagcagcagc ggcggcaatt cctatgccgg
     6601 tccgcgcgcc ggtccgcgcc cgatgccggc gctgcaatcg ccgatcggcc tgcagaaccg
     6661 ccccggcggc acggtgaccc gcaagcgcat gggcaagccg ccgcccaagc cgggcgagct
     6721 ggacgatccg gaccaggccg gcgaagacgg caccggcggc gccccgcgcc ccgacatgag
     6781 ccgcctcgaa gaggtcaagc aggcctcgcg ccagcgctca ggcagcgcca acgccgatgg
     6841 cgactagaag cactcccggg ccaggaaggc gcgcgaatcc gcgccgatca gcgcggcgtg
     6901 gccatagttc gggtggtcga tcaccagcat cgcgttgcct tccccgctct tccacgcggc
     6961 aatcgccgcg gccgggaaat cgaagtggaa gaagtgcacc gcactggcct tgttgtcgct
     7021 ttcgcgggtc cgctcggtat cgccttcggg gcgggcccgg ataaggaaat cgccgacctg
     7081 gatcgcgacg tggttttcca ccccgccgat cgtccgcagg aaagcatcgc ggcgcaccgg
     7141 atcgccgatc tcgaacagca gggtgcaggt cagctcgctg cccttgggca ccatcgggtc
     7201 ataggcggcc agctcgtcgc tgagctgctc gtccccgccc ttttcgatcc gcagcatctc
     7261 ctggacctgc agccacatcg aatcccagtt ttcgaatagc accgtcgcgt gcgggccgac
     7321 cgcaatgcgg gtcagtgcct tgcgcgccag ggcctcctgc ttcttgtcgg cccggatcag
     7381 ttcgtactgg tcgagcggca ggatgtcgtc gcgggtaatc gtgcgggtat cgcggggcat
     7441 ggtcagttcc tagagtccat aggcttgggc gaggacctcg atcgggtggc cgatccgggc
     7501 cggcggttcg gccccgttcg cggcaatcac ctgcttgagg tgcggaccgg cgagcgggca
     7561 ttcggacacc accagatcgg gctggtcctt catcaggttg cgcgcggcag gcttgcccac
     7621 cttgaccgcg cggtcgaagt tttccttgaa gatcccccac ttgccgccgt ggcccgagca
     7681 gcgctcggtc agggctggct tggcctcggg gatcaggcgc agcatttcca tcgccttggg
     7741 acccatgttc tgcgcccggg cgtggcaggc aaagtgcacc gcgatgcgct tgtccatcgg
     7801 ctcgatcggg gccagcccgg tgtccttcga gaggccgacg atgtactccg caacgtcgaa
     7861 ggtgtggcgg ctgagcagct tgaccgtctc gttctccggc tcgatcagcg gccattcgaa
     7921 cttcagcatc agcgcgcagg aggtggtcag cgccaccacg tcccagccct gctcgatcag
     7981 cggcgcaaag tgcgcggaaa tgcgggtcgc ggcgcttgcc acggccggca gatcgccgtt
     8041 ctcgaacttc ggcatggcgc agcagtccgg atgctcgatc cgcacctgga cgccgttgtg
     8101 ggccaggacc ttgaccatcg ccaggcccgg gccgccatcg ttgaactcgt catggcagcc
     8161 ggcatagatc gccaccttct tgccgaaggc cggaccatcg ggattgggct gcggcacgat
     8221 cgcgacggcc tggcgggtca gcgaaacatc catgaacggc ggcaaggcag cgcgccggtc
     8281 aatcccggtg accttttcga tcacggggcg ggtcaggctg ttgtcttcct tggtggccca
     8341 gttgaccgcg cccgacagga tcgtaccgag acggccgttg cggtccattt cggccagctg
     8401 cttgtcggcc agcgaagtgc cgcccttgcg gtgctggacc gcgcggtggc gcagcatcag
     8461 gtgcgggaaa tccagatcga agctgtgcgg cggcacatag gggcacttcg tcatgaagca
     8521 catgtcgcac agggtgcagg catcgacgac ctgcgccagc tgcaccgggg tcagatcctc
     8581 gacctcctcg ttcggggcct cgtcgatcgc gtcgaacagg atcggaaaac tgtcgcacag
     8641 gttgaagcag cggcggcagc cgtggcagat gtcatagacg cggcgcagct cggcctcgag
     8701 cgcggcttcg tcgtaccagc tttcatcctg ccacggaatc gggtggcggg tgggcgcttc
     8761 aaggctgcct tccatcgtgg cggtcccctg tttgcttcac agcaatcgca aatgtgcgga
     8821 atttaaagac aggacgggcg gcaggcgcgc aaaccgcgcc tgccgcccgc tggtaagatc
     8881 acgcgtcggc gagcaggccg tcgagggtct tctggaactt gccggcgtgg ctcttttccg
     8941 ccttcgccag ggtttcgaac cagtcggcga tttcgtcgaa accttcgtcg cgggcggtct
     9001 tggccatgcc cgggtacatg tcggtgtact cgtgggtttc gccggcgatc gacgccttca
     9061 ggttggcaac ggtgtcaccg atcggttcgc cggtggccgg atcgccgcac tcttcgaggt
     9121 attcgaggtg gccgtgggcg tggccggttt cgccttccgc ggtcgagcgg aacacggcgg
     9181 caacgtcgtt gtggccttcg atgtccgcct tctgggcgaa gtacaggtaa cggcggttgg
     9241 cctggctttc accggcgaac gcggccttga ggttttcctc ggtcttcgaa cccttgagtc
     9301 ccatgatgtc tctccaaagt ttgggtggaa tccaaccacg cttcaatgcg ccggagagcg
     9361 gcccaaggcc atgcggaaat tccgcgacca gtaatcggct aaaccgatca tattcttgcg
     9421 aataattcgc aattacgatg gatggtgccc ctggcccgac tcgaacgggc acgccttgcg
     9481 acaaacgatt ttgagtcgtt cgcgtctacc attccgccac aggggcatct tgttccgtgc
     9541 gccctcgaca tccgtcgatg gcttggcagc atccgccgtc aggcggacaa aatcacctat
     9601 cccgccttca gcgcgccgac aatcaccttg tgcaggcgcg aatgcagcgc gtcgttgccg
     9661 gccaggacct gcttgtcgca gaccggctgg ctgatcccgc gccagtcgga cacgaacccg
     9721 cccgcctcgc gcaccagcag acagccggcg gccgtatccc acggcgaaag cccgctttcc
     9781 cagaagcctt catagcggcc cgcggcaacc caggcgagat caagcgaggc cgcgccgaag
     9841 cggcggatcc ccgcgacctg cggggccagc gcggaataga tcttcatcca ttcggccggt
     9901 tcgcccgctc cggcaaaggg aatgccggtg gcgatcaggc tttcgtcgag gtgccggcgc
     9961 gccgagaccc gcaggcggcc gtcgtgcagc caggcgccgc ggctcttttc ggcccaatag
    10021 ctttcgtcgg tgatcggctg atagaccagc gccgcggtca catcgcccca gcccgaacca
    10081 tcgagcttgg gttcctgcac cgcgatcgag atcgcgaagt gcgggatgcc gtgaaggaag
    10141 ttgctggtcc cgtcgagcgg atcgacgatg aagcgcggct tgccttcggc gccgtgaatc
    10201 tccccgcctt cctccatcag gaagccccag tccggccgcg cggcgcgcag ttcgtcccag
    10261 atcgtccgct cggcgcgctg atcggccttg gacacgaaat cggccggccc cttgcggctg
    10321 acctggagat gctcgatctc gccaaagtcg cgacgcaagc ggccacccgc cttgcgggcg
    10381 gccttttcca tcacgcggat gattccggaa acgacggcca tgattacttc cgttcctcat
    10441 ctagcgagac agccacgccg tccgcttgaa agttcaattt gaaaatttcg gtttcgatct
    10501 caaaatgatc acaccgaatt gcgacgtgat caaacgtccc gaaatcgtaa cagtccagag
    10561 agcgcggcaa ggtcttgccc gtctgctcca aactcaattc gcggcaattg cgaaactcca
    10621 gccaccctga tcggtagcaa gcccactccc cctccttggc ggggcggtgg cccgggtggt
    10681 cctgcgtaat gtgcagatcc acccgcagtt tcaggctgct tccttccatc agaagcccct
    10741 tcaaccagga gtcctggaga ttgaggccaa caagggaagt ggccgccttg gccatcgcag
    10801 atcagtccgc cttgccgacg tagctgcgct cgtacacgtc gacgacgatc cgggtgccgc
    10861 tggaaatgtg cggcggcacc atcacgcgca cgccgttgtc gaggatcgca ggcttgtagc
    10921 tcgacgaagc ggtctggccc ttcaccacgg catcggcttc gacaatcgtc gcttcgatgt
    10981 gctgcggcag ctcgacgctg accggacgtt cctcccacag ttccagcacc acatccatcc
    11041 cgtcctgcag gaagtccgcg gcatcgccga gcaggtcgcg cggcagggtg atctgttcga
    11101 agttgtcctt gtccatgaag accaggtcat cgccttcggc gtagaggtac tggaaatcct
    11161 tggtgtcgag gcggaccttt tcgaccgtgt cggcgctgcg gaagcggacg ttggtcttgc
    11221 gcccgtccat caggttcttc atctcgacct gcatgaacgc gccgcccttg ccgggctggg
    11281 tgtgctgggt cttggcgacc ttccagatac ccttttcgta ttcgatgatg ttgccgggac
    11341 gaatgtccac gccgctgatc ttggccatga ctgcctcttc aaggatgaga aagagcccgg
    11401 ctgcaacggc cggaaggttc gcgcccttag cgcgtcagtc cgtgcggggc aaggtggcgc
    11461 gttggcttcg gcggtgctag gaggtgcggc gatgcttcgc tacgcccttc ccgccgcgct
    11521 tcttgccgcc tccgccgccg ccctggccgt gccgggcgga ccgatcgggg tgctcgcccc
    11581 ggccagctat gcctgcgaga tgccgggcga tgccgtctcc gccgccggat accgggtcga
    11641 tgcggagaac ttcacgatcg tcaattccaa cacctattac accgccagcg gccgcggcac
    11701 ctatctgctg accggcgatc tgctggtgct gaccagcgga cccaagcgcg gccagaaatt
    11761 ccgcaagatc agcgccaatt tcctgcgcaa gctggggccg gacggatccg agaccgcgct
    11821 gcgctgcgtg cgccgggtgg tcaacaacag caacggcagc ggctgtgaca gcagcgccgc
    11881 cggcagcacg ccgggcgcca gctgccccgc accgcgccgc taggctaggc cgccgtccgc
    11941 cgcgtccagc ggtagagcca ggtgacggca ccggcaaaga ccagcgctcc cagcagcggc
    12001 gtccaggcgc cgctttccac cacggccagc gcctcgggac tgccggttgc tgcggcgatc
    12061 ccggcaaaca gcacgccgaa caggctttcg cccacgatca ggccggttgc ggccagcacg
    12121 cccatccgct cggcaaaggc cggttcggcc tggcgctcgg cccagcggtt gtagcggtgg
    12181 ccaaggtacg cccccagcgg gatcagcacg gtcaggctca tcggcaggta catgcccatg
    12241 cccacggcca gcggcggcag gctgcccttg ccggtcttgc gcagggcctc gtccaccagg
    12301 atcaccccgg ccccgatccc tgcgccaagg ccgatcaggc cccagtcgag attgccgccc
    12361 agcacccctt gcgcgatcgc cgtgatcagc gctgcctgcg gagcggccag ggcatccgcg
    12421 ccggccccgg gcgcgccctg gaagccgaag gtggtgttga gcaggtccag gatcggcggg
    12481 atcacgatgg ccccgaagat cacccccagg atcagcgcga cctgctgacg ccacggcgtc
    12541 gcgccgacca gttgcccggt cttgaggtct tgcaggttgt cgttggaaat ggtggcgatg
    12601 ccgaacacga tggcggttac gaacagcgcg aaggccacca gcgcccgggt tgaggcttcg
    12661 tctccgccct gcccgaagat cgccaccagc agcagcgaga tccccaggct ggcgaggatg
    12721 ccgacgccgg aaatcgggct gttcgacgcc ccgatcagc
//
LOCUS       DASUDV010000335         7444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2175868,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000335 DASUDV010000000
VERSION     DASUDV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175868"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..616
                     /locus_tag="VFV30_07305"
     CDS             71..616
                     /locus_tag="VFV30_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847935.1"
                     /translation="MENFEFTFTLFGLLLGLALTEPLAGIARALKSRHRVRIGWPTAL
                     LGLTVSCDVVTFWMYGWSLRDEIKVSWPILFGGFLVTAVYYICAAMIFPDSVDEDHEA
                     HFDRVKPLVLGGMVLCNAALLGFVVHLVGLEPLLNGRAVIITWSLFPMAIAAALIKDR
                     RVVIACLLWLIALYPLSAFWH"
     gene            complement(633..1466)
                     /locus_tag="VFV30_07310"
     CDS             complement(633..1466)
                     /locus_tag="VFV30_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX4847936.1"
                     /translation="MNSSPWSHHTRNPGIADDIDFEIKGQELQFLEIELDPGESAVAE
                     AGAMVWKDATVQMSTVFGDGRGAEGGFMGKLLGAGKRLVTGESLFTTVYTHTGSGKAR
                     VAFSAPVPGAILPIKLDQVGGTLICQKDSFLAAAKGVEIGIHFQRRILTGLFGGEGFI
                     MQRLSGDGWVFVQMGGTIVERELRPGEELHVDTGCIAAMTPNVEFEIQTVGGVKSMLF
                     GGEGLFFARLRGPGKVWIQSLPFARLAGRMLQAAMPGGPGGNRGEGSALGMLGNLLDG
                     D"
     gene            complement(1543..2103)
                     /locus_tag="VFV30_07315"
     CDS             complement(1543..2103)
                     /locus_tag="VFV30_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847937.1"
                     /translation="MNPFKLVAGFVRWLWSLSMAVRHPIDSGLRTGSASAGLYMFLFL
                     LFLVIGLVLMALGFDLADIDRWLDANGGWIEGGADLLFRAFMGLIFLFSLLAAGVMLF
                     EMGRALFGARGGALDDPEAQLERAQMAEDDRMLAAELADPDEPLEDFAPPEPDAKDEP
                     TGPLGLGCGMLVALVFAYFAWFGMTG"
     gene            complement(2132..4066)
                     /gene="uvrC"
                     /locus_tag="VFV30_07320"
     CDS             complement(2132..4066)
                     /gene="uvrC"
                     /locus_tag="VFV30_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX4847938.1"
                     /translation="MTKAGRPDQPNAADRFNEERATYTVKGGQPDLEAGTAAIREVQA
                     TLKPKPGVYRMTDAKGDVLYVGKARALKNRVANYLQVDRLPLRLKRMVSQTRGMTIVT
                     TNSEAEALLLEAQLIKRYRPPYNVLLRDDKSFPFILLRADHDFPRIMKHRGARRAKGN
                     YYGPFASAGSVNTTINALQKLFLLRSCTDSFFARRDRPCLLYQIKRCAAPCVGRIDAA
                     GYGELVAEAKDFLGGKSSAVQAKLNQQMEEAAAALDFERAAMLRDRLRAATFIQGSQA
                     VNAEGVGNADVFAVAEKGGHFAVQAFFIRGGQNWGHRAFFPGHTEGLDAAEVLTAFLA
                     QFYEEVPPPRLVLVDRTLPEAALLAEALAGAAGGKVEVSVPQRGDRRRLMEQASRNAV
                     EALDRRAAESGTKARLNRELAEFLELPEPPQRIEVYDNSHIQGANALGAMIVAGPDGF
                     IKGQYRKWNIKAAQTNDDYGMMREVFQRRFARAQDEDPDRDGGTWPDLVLIDGGKGQM
                     SAARDTLEEMGIEDVPLIAIAKGPHHGREGREVFHFPDGREKMLPVNSPLLFHLQRLR
                     DEVHRFAIGAHRDKRSRAITASPLDEIPGIGPARKRALLLHFGTASKVRAASLEDLQR
                     APGVSAAVAQAVYDFYHPGG"
     gene            4189..5742
                     /locus_tag="VFV30_07325"
     CDS             4189..5742
                     /locus_tag="VFV30_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX4847939.1"
                     /translation="MKQLQGMDASFVALETRNSPMHIGSLLIYNPETAPGGFVRYKDI
                     LRFFESRMQLSKTMRQRLVRVPFDLDYPYWVEDKDFDLEYHVRHVALPRPGDWRQLCI
                     QAARIFARPLDLTRPPWEFTIVEGLDNIPGVTPGSYAMVTKVHHAAIDGMSGIDLMEA
                     LHTLDPNAPPPNQPDTWKGEDVPNFAELLTKSWINNLANPLRQLDVAAKAMPGVAKAI
                     KGLVTKDFKLHGEMLAPRTRFNAVISPNRVVEGRSIPLADIKAIREAVPGAKVNDAFL
                     AIIGGAMRKYLEHHGDLPDKTLTAMAPISVRAKDEKGDMGNQVAAMIAPLGTHLADPL
                     ERLAYVHSQTVNSKAMTDAIGARNMTEMSKVSPALFMALGAQLYTRLGLANRVGPPFS
                     TVVTNVPGPPVPIYSAGARLESMMGLLCLTDGLDLGHVVQSYVKEATIAFTACRKLMP
                     DPDFYASCIEASFNELRDAAAAAVAGGAKPAEPKPKAAKPAPKAKAAKTKTAAKKGAA
                     KPGKAAKTS"
     gene            5739..6542
                     /locus_tag="VFV30_07330"
     CDS             5739..6542
                     /locus_tag="VFV30_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847940.1"
                     /translation="MTATTAAREALDIEARPPHRLLALAEGRAVFELASFYATRHLLG
                     MLPRGDGHPVLVLPGFLASDSSTAPMRRLLKDLGYEAHGWGLGRNVRVDLERVHEMEA
                     LLKRIHAESGRKVSIVGWSLGGVFARELAKLHPDMVRQVISLGSPIHDDRRHTNASRL
                     FEFLNGKEPEPIKHGRFTGLDQAPPVPTTSILTRSDGIVHWRGSVQHADRGHGQTENI
                     VVHASHCGLGVNPSVMVAIADRLHQDEGTWKPFQPRLSQRWMFPAQRLH"
     gene            6542..>7444
                     /locus_tag="VFV30_07335"
     CDS             6542..>7444
                     /locus_tag="VFV30_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4847941.1"
                     /translation="MSTRHLVDPSLLPLLEFMPGGGFSAETLPEIRVQSEQRFAFLGA
                     PVLEPQVKVIDGPGGPLEIYWYDPDPTAGVRPALYHIHGGGMVIGSAKSMQHGPAGMA
                     AALGIPVASVEYRLAPEHPFPAPQEDCLAGLAWLAANAAELGVDPARIGIVGESAGGG
                     LAAATALMARDLGGPALAAQFLVFPMLDHRTGSEDCPYANPTTGEFVWTRDSNRFGWG
                     ALRGDYAADDHRKGWFSPSLSESLAGLPQTWIGTGSLDLFLDENLDYARRLIAAGVPV
                     ELHSYPGAIHAFQAIADGHLAKAFN"
BASE COUNT         1200 a         2582 c         2450 g         1212 t
ORIGIN      
        1 ccccgaaggc gacgcgcgcg aggcgttcta caagcaggtc caggccctga tcgacgcggg
       61 gggctgagcg gtggagaact tcgagttcac cttcaccctg ttcggcctgc tgctgggcct
      121 ggcgctgacc gagccgctgg ccggaatcgc ccgggcgctc aagagccggc accgggtgcg
      181 gatcggctgg cccactgccc tcctcggcct caccgtctca tgcgatgtgg tgaccttctg
      241 gatgtacggc tggtccctgc gtgatgagat caaggtcagc tggccgatcc tgttcggcgg
      301 gttcctggtc accgcggtct attacatctg cgcggcgatg atctttccgg acagcgtgga
      361 cgaggaccac gaggcccatt tcgaccgggt caagccgctg gtgctgggcg gcatggtgct
      421 gtgcaacgcg gccctgctcg gctttgtcgt ccatctggtc ggactggagc cgctgctcaa
      481 cggccgcgcg gtgatcatca cctggtcgct gttcccgatg gcgatcgcgg cggccctgat
      541 caaggaccgc cgcgtggtca tcgcctgcct gctgtggctg attgcgctct atccgctatc
      601 ggccttctgg cactaggcgg caggccgcag cctcaatccc cgtcgagcag gttgccgagc
      661 atccccagcg ccgatccctc gccgcggttg ccgccggggc cgccgggcat ggccgcctgc
      721 agcatccgcc cggccaggcg ggcaaacggc agcgactgga tccagacctt gcccggaccg
      781 cgcaggcggg caaagaacag gccttccccg ccgaacagca tcgacttgac gccgcccacg
      841 gtctggatct cgaattccac attgggggtc atcgcggcaa tgcagccggt gtcgacgtgc
      901 agctcctcgc cggggcgcag ctcgcgctcc acgatggtgc cgcccatctg gacgaagacc
      961 cagccatcgc ccgacaggcg ctgcatgatg aagccctcgc cgccaaacag gccggtcaga
     1021 atccggcgct ggaaatggat gccgatctcg acgcccttgg ccgcggccaa gaagctgtcc
     1081 ttctggcaga tcagcgtgcc gccgacctga tcgagcttga tcggcaggat cgcgccggga
     1141 acgggcgcgg aaaaggcaac gcgggccttg ccgctgccgg tgtgggtata gaccgtggtg
     1201 aacaggcttt ccccggtcac cagccgcttg cccgcgccca gcagcttgcc catgaagccg
     1261 ccctcggccc cgcgcccgtc gccgaacacg gtgctcatct gcaccgtggc atccttccac
     1321 accatcgccc cggcctcggc caccgcgctt tcgcccggat cgagctcaat ctcgaggaac
     1381 tggagctcct gccccttgat ctcgaagtcg atatcgtcgg caatgccggg gttgcgggtg
     1441 tgatggctcc aggggctgct gttcatcggt ggtcctctct ccgaatggga tggcgcagat
     1501 atatggctct tgcggcccgc gccaaggggg gcggtgaaat tgctagccgg tcatcccgaa
     1561 ccacgcgaaa taggcgaaga ccagcgcgac cagcataccg cagcccagcc ccagcgggcc
     1621 cgtgggctcg tccttcgcat cgggttcggg cggggcaaag tcttccagcg gctcgtccgg
     1681 atctgccagt tccgcggcca gcatcctgtc gtcctcggcc atctgggcgc gttccagttg
     1741 cgcctccggg tcgtccagtg cgccgccccg cgcgccgaac agcgcccggc ccatttcgaa
     1801 cagcatgacc ccggcggcaa gcaggctgaa caggaagatc agccccatga aggcacggaa
     1861 cagcagatcg gccccgccct cgatccagcc gccgttggcg tccagccagc ggtcgatatc
     1921 ggcaaggtca aagccgagcg ccatcagcac caggccgatc accaggaaca gcaggaacag
     1981 gaacatgtag agcccggcac tggcgctgcc ggtgcgcagt ccggaatcga tcggatggcg
     2041 caccgccatc gacaggctcc acaaccagcg gacaaagccc gcaaccagtt tgaacggatt
     2101 catcgatcgt ccccccggca gccggcctgc gctacccccc cggatggtag aagtcgtata
     2161 ccgcctgcgc aaccgccgcg ctgacgccgg gcgcgcgctg caggtcttcc agggatgccg
     2221 cccgcacctt gctcgccgtg ccgaagtgga gcagcagggc gcgcttgcgg gccggcccga
     2281 tgccggggat ttcgtccagc gggctggcgg tgatcgcacg gctgcgcttg tcgcggtggg
     2341 cgccgatcgc gaagcggtgc acctcgtcac gcaggcgctg caggtggaac agcagcgggg
     2401 aattgaccgg cagcatcttt tcccgcccat cggggaagtg gaacacctcg cggccctcgc
     2461 gcccgtggtg cgggcccttg gcaatggcga tcagcggcac gtcctcgatc cccatttcct
     2521 ccagcgtgtc gcgcgccgcc gacatctgcc ccttgccgcc gtcgatcagg acaagatcgg
     2581 gccaggtccc gccgtcgcgg tccggatcct cgtcttgcgc gcgcgcgaac cgccgctgga
     2641 agacctcgcg catcatgccg taatcgtcgt tggtctgggc cgccttgatg ttccacttgc
     2701 ggtactggcc cttgatgaac ccatcgggtc cggcgacgat catcgcgcca agggcattgg
     2761 cgccctggat gtggctgttg tcatagactt cgatccgctg cggcggttcg ggcagctcca
     2821 ggaactccgc cagctcgcgg ttgagccggg ccttggtgcc gctttcggca gcgcgccgat
     2881 ccagcgcttc caccgcgttg cggctggcct gctccatcag ccggcggcgg tcgccgcgct
     2941 gggggacgga gacttccacc ttgccaccag ccgctccggc aagcgcctcg gccagcagcg
     3001 cggcttcggg cagggtccgg tcgaccagca ccagccgggg cggcggcact tcctcataga
     3061 actgggcaag gaacgcggtc agcacctcgg cggcgtccag cccctcggtg tggccgggga
     3121 agaaggcgcg gtgcccccag ttctgcccgc cgcggatgaa gaaggcctgg accgcgaagt
     3181 gcccgccctt ctctgccacg gcgaagacat cggcattgcc gacgccttcg gcgttcaccg
     3241 cctggctgcc ctggatgaag gttgccgcgc gcagccggtc gcgcagcatg gcggcgcgct
     3301 cgaaatcgag cgcggcggcc gcctcttcca tctgctggtt gagcttcgcc tggacggccg
     3361 agctcttgcc gccgaggaag tccttcgcct cggccaccaa ttctccatag ccggccgcgt
     3421 cgatccgccc gacgcagggc gccgcgcagc gcttgatctg gtagagcagg cacggccggt
     3481 cgcgccgggc aaagaagctg tcggtgcagg accgcagcag aaacagcttc tgcagcgcgt
     3541 tgatcgtggt gttgaccgat ccggcgctgg caaaggggcc atagtaattg cccttggccc
     3601 gccgcgcgcc gcggtgcttc atgatccgcg ggaaatcgtg atcggcgcgc agcaggatga
     3661 aggggaagct tttgtcgtcc cgcagcagca cgttgtaggg cgggcggtag cgcttgatca
     3721 gctgcgcttc cagcagcagc gcctcggcct cggaattggt ggtgacgatg gtcatgccgc
     3781 gggtctggct gaccatccgc ttcagccgca gcggcaggcg atcgacctgc aggtagttcg
     3841 ccacccggtt cttcagcgcc cgcgccttgc cgacatagag cacgtcgccc ttggcgtcgg
     3901 tcatccggta aaccccgggc ttgggcttca aggtggcctg cacttcgcgg atcgcagcgg
     3961 tcccggcctc caggtccggc tgcccgccct tgacggtata ggtcgcccgc tcctcgttga
     4021 aacggtcggc agcgttgggc tgatcggggc gtccggcctt ggtcatcgcc ctccgcgaat
     4081 acagggcgtc ccgccggggc gaaagccgcg agttgacggc aatcccccgt gtgttacgct
     4141 gcgccgcaca cgacccgcca aacgggccgc aggagggaga ccaggcgctt gaagcaactc
     4201 caggggatgg atgcatcgtt cgttgcgctg gagacgcgca attcgccgat gcacatcggc
     4261 tcgctgctga tctacaatcc ggaaacggcc cccggcggct tcgtgcgata caaggatatc
     4321 ctgcgctttt tcgaaagccg gatgcagctg tcgaagacca tgcgccagcg gctggtgcgg
     4381 gtgcccttcg atctcgatta cccctattgg gttgaggaca aggacttcga cctggaatac
     4441 cacgtccgcc atgtcgccct gcccagaccg ggcgattggc ggcagctgtg cattcaggcg
     4501 gcgcggatct ttgcccgtcc gctcgacctg acccgcccgc cgtgggaatt caccatcgtc
     4561 gaagggctgg acaacattcc cggtgtcacc cccggctcct acgccatggt caccaaggtc
     4621 caccacgccg cgattgacgg gatgagcggg atcgacctga tggaggcgct ccacacgctc
     4681 gatccgaacg ccccgccgcc gaaccagccg gacacctgga agggcgagga cgttcccaac
     4741 tttgccgagc ttctgaccaa gagctggatc aacaacctcg ccaacccgct gcgccagctg
     4801 gacgttgcgg ccaaggcgat gccgggcgtg gccaaggcga tcaagggcct ggtcaccaag
     4861 gatttcaagc tccacggcga aatgctcgcc ccgcgcaccc gtttcaacgc ggtcatctcg
     4921 cccaaccggg tggtcgaagg ccgctcgatc ccgctggccg acatcaaggc catccgcgag
     4981 gcggtgccgg gggccaaggt caacgatgcc ttcctggcga tcatcggcgg ggcgatgcgc
     5041 aagtacctgg agcatcacgg cgacctgccg gacaagaccc tgaccgcgat ggccccgatc
     5101 tcggtccgcg ccaaggacga gaaaggcgac atgggcaacc aggtcgccgc gatgatcgcg
     5161 ccgctgggca cgcacctggc cgatccgctg gaacggctgg cctatgtcca ttcgcagacg
     5221 gtcaattcca aggcgatgac cgacgcgatc ggcgcccgca acatgaccga gatgagcaag
     5281 gtcagcccgg cgctgttcat ggcacttggc gcgcagctct atacccggct gggcctggcc
     5341 aaccgggttg gcccgccctt ctccaccgtg gtcaccaacg tgcccggccc accggtgccg
     5401 atctattcgg ccggcgcccg gctggaaagc atgatgggcc tgctctgcct gaccgacggg
     5461 ctggacctgg gccatgtggt gcaaagctat gtgaaggagg cgacgatcgc cttcaccgcc
     5521 tgccgcaagc tgatgcccga tccggatttc tacgcatcgt gcattgaggc cagcttcaac
     5581 gaactgcgcg atgctgcggc tgcggccgtt gcgggcgggg caaagcccgc cgaaccgaaa
     5641 ccgaaggccg ccaagcccgc ccccaaggcc aaggcagcca agaccaaaac tgcggcgaaa
     5701 aagggcgccg cgaagcccgg aaaggctgcg aagacatcat gaccgccacc actgccgcgc
     5761 gcgaagcgct ggatatcgag gcccgcccgc cgcaccgcct gctggcgctg gccgaagggc
     5821 gcgccgtgtt cgagctggcc tcgttctatg ccacccgcca cctgctgggc atgctccccc
     5881 ggggcgatgg ccacccggtg ctggtcctgc ccggatttct ggcctcggac agctcgaccg
     5941 cgccgatgcg ccggctgctg aaggatctgg gctatgaggc gcatggctgg ggcctgggcc
     6001 gcaacgtccg ggtcgatctg gaacgcgtcc atgaaatgga agccctgctg aagcggatcc
     6061 atgccgaaag cggccgcaag gtctcgatcg tcgggtggag cctgggcggc gtctttgccc
     6121 gcgaactggc caagctgcac ccggacatgg tccggcaggt gatctcgctg ggcagcccga
     6181 tccacgacga ccgccgtcac accaacgcca gccggctgtt tgaattcctg aacggcaagg
     6241 agcccgagcc gatcaagcac ggccgcttca ccgggctgga tcaggccccg ccggtgccga
     6301 ccacctcgat cctgacccgc agcgacggga tcgtccactg gcgcggctcg gtccagcatg
     6361 ccgaccgcgg ccacggccag accgagaata tcgtggtcca cgccagccat tgcggcctgg
     6421 gggtcaatcc cagtgtgatg gtggcgattg ccgaccggct gcaccaggac gaaggaacgt
     6481 ggaagccgtt ccagccgcgt ctgtcgcagc gctggatgtt ccctgcccag cgcctgcact
     6541 gatgtccacg cgccatctgg ttgaccccag cctgctgccg ctgctcgaat tcatgccggg
     6601 cggcgggttc agcgcggaaa ccttgcccga aatccgggtc cagtccgaac agcgctttgc
     6661 cttccttggc gcgccggtcc tggagccgca ggtcaaggtg atcgacggcc ccggcgggcc
     6721 gctggagatt tactggtacg atcccgatcc cacggccggc gtgcgcccgg cgctgtacca
     6781 tatccacggc ggcgggatgg tgatcggttc ggccaagtcg atgcagcacg gcccggccgg
     6841 catggcggca gcgctgggca taccggtcgc ctcggtcgaa taccggctgg cgccggagca
     6901 cccctttccc gccccgcagg aggattgcct ggccggtctg gcctggttgg cggcaaatgc
     6961 cgcggagctg ggggtcgatc cggcacggat cggcatcgtc ggtgaaagcg ccgggggcgg
     7021 ccttgccgca gccacggcgc tgatggcgcg cgatcttggc ggaccggcgc tggctgccca
     7081 gttcctggtc ttcccgatgc tcgatcaccg caccggcagc gaagattgcc cttatgccaa
     7141 cccgacgacc ggcgaattcg tatggacgcg ggacagcaac cggttcggct ggggcgcgct
     7201 gcgcggtgac tatgccgcgg acgatcaccg caagggctgg ttctcgccca gcctgtccga
     7261 aagcctggcc ggcctgcccc agacctggat cggcaccggc agcctggatc tgtttctgga
     7321 cgagaacctg gactatgcgc gccgcctgat cgccgcgggc gtgccggtgg aactgcattc
     7381 ctaccccggc gcgatccacg cgtttcaggc gattgccgac ggccacctgg ccaaggcgtt
     7441 caac
//
LOCUS       DASUDV010000336         5239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2131598,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000336 DASUDV010000000
VERSION     DASUDV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131598"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1130
                     /locus_tag="VFV30_07340"
     CDS             84..1130
                     /locus_tag="VFV30_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847942.1"
                     /translation="MRKSLLMPVTLVSLSFGVATLALANPLDRAGSIRGQKPAPSRPA
                     AAQPAGPIPDQGVHNAVHAAHQNQIVFTRIDTTVGGITEADIVRDFTLGQPMFFRVYM
                     ERSAVNAIAAATGMPASAVYADGVHYTARFTIDGQTFDTTMFPWGNPRDHQTWTTWRG
                     QFINPGTAQRTPGSDAFFEMLSRATSAGLLKPGKHTIKMELIAKTNTEKAGPISAGVV
                     ASGTFNLTVPAGIFSPSNTTICGARRGGAGSAAMEARALSEAKRISRNPELVPVRAIG
                     IGESWDVERNAVTGIPIERETYVLIQSRGPKFCTSNTHSFYEDYMGGGSFSTATGSIS
                     VNMRVGYVPCGCLP"
     gene            1135..1656
                     /locus_tag="VFV30_07345"
     CDS             1135..1656
                     /locus_tag="VFV30_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847943.1"
                     /translation="MTRTFHAPRPVRRPYSPLFMALRQACSTLIEQMERKGAVALPPL
                     DRPFQGEEWEAYHPGLILATVDWDVPRLVFNWAVDDDRPVRAARSPDYTIDLAALKSA
                     ARADPDPRGWFESQAAYALSLAQNYLIDFFRDGPTDDIPFERHLIFVRGLEGLDGTLW
                     IKNVLFAFEETPG"
     gene            1711..2124
                     /locus_tag="VFV30_07350"
     CDS             1711..2124
                     /locus_tag="VFV30_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847944.1"
                     /translation="MLVCNETHFQGIAPAIRALVERMEADWLALGFAKEDAVFVDVNA
                     TNGHLMLTWDKSGGGGSFYDENWPVYYLELKALWRESLDHPEGAYHFDNHAHIALCSV
                     VEALLIEAEAADQPERYEFYELFEGSTNGAVRVLV"
     gene            2121..2549
                     /locus_tag="VFV30_07355"
     CDS             2121..2549
                     /locus_tag="VFV30_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847945.1"
                     /translation="MTGTVAMRSQATGWRGLADDLLRAIDWLDAHGAAGEPAIRITVA
                     ADRRSLSLCACDAPGAPVLTLALGVLPETTDTAREDGQRPPNAAIDRAVCFAAAAMLS
                     WSKDPETFELLTHQRHALLLAWDDGPASPVDPWAYGLWDG"
     gene            complement(2605..2679)
                     /locus_tag="VFV30_07360"
     tRNA            complement(2605..2679)
                     /locus_tag="VFV30_07360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2645..2647),aa:Thr,seq:ggt)
     gene            2793..2927
                     /locus_tag="VFV30_07365"
     CDS             2793..2927
                     /locus_tag="VFV30_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847946.1"
                     /translation="MREDQPPLTREERLAAKLRENLRRRKEQARALGAQVLPKDDPTR
                     "
     gene            2979..3665
                     /gene="gpmA"
                     /locus_tag="VFV30_07370"
     CDS             2979..3665
                     /gene="gpmA"
                     /locus_tag="VFV30_07370"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX4847947.1"
                     /translation="MPRLILVRHGQSQWNLENRFTGWWDVDLTAKGEDEARAAGALLK
                     AKGMLPTVAFTSLQTRAIRTLHFALESAGRLWIPETKDWRLNERHYGGLTGLDKAETA
                     AKHGDEQVKIWRRSFDTPPPVLEDGSEFDLKADPRYAGIAIPSTESLKDTIARVLPYY
                     EAEIAPALKAGEVVLVAAHGNSLRALVKHLSGISDAEITGLEIPTGQPIVYELDDTLA
                     ARERYYLAER"
     gene            3667..4146
                     /gene="purE"
                     /locus_tag="VFV30_07375"
     CDS             3667..4146
                     /gene="purE"
                     /locus_tag="VFV30_07375"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX4847948.1"
                     /translation="MNAAVAIVMGSQSDWATMKCAADALDKLGVSYDARIVSAHRTPE
                     RLVKFAQGAADEGFKVIIAGAGGAAHLPGMVASMTHLPVLGVPVQSKALSGQDSLLSI
                     VQMPAGIPVGTLAIGEPGATNAGLLAAAILATTDPALAERIKAFRAAQSAGVAERPS"
     gene            4163..5218
                     /locus_tag="VFV30_07380"
     CDS             4163..5218
                     /locus_tag="VFV30_07380"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX4847949.1"
                     /translation="MIAPGETIGILGGGQLGRMLAKAAAQLGYRVHVYAPEADSVAAE
                     VCAQFTCAAWDDARAMATFAADCAVVTYEFENVPVGPLKALGSVPLHPNARALEVAQD
                     RLNEKEFVEQLGGRTAPYMAVDTREDLDEALVRIGSPGILKTRREGYDGKGQWRINYD
                     HEAEGIALGAQPLIFEGLVRFSAEFSVILARGQDGAVVFWDSAENVHEHGLLARSTAP
                     ASPAVQAQVEAARALAKKVADGLGYVGVAAFEFFATEDGPVFNEMAPRVHNSGHWTIE
                     GAVTSQFENHIRAICGLPLGDTRLAAARVEMRNIIGDAAEDWAAVLADPANHLHLYGK
                     GAARPGRKMGHITRLTF"
BASE COUNT          879 a         1797 c         1776 g          787 t
ORIGIN      
        1 ccgggcaacc ccgcgctttg gggggatggt cgcgcacggg gggtggctgc agacaccggg
       61 ggaacgtcac gtggagccgc accatgcgca agtccctgct aatgccagtc accctggtct
      121 cgctgtcctt cggtgtcgcg accctggcct tggcaaaccc cctggaccgg gccggcagca
      181 ttcgcggcca aaagccggcc ccgtccaggc cggctgccgc ccagccggcg ggtccgatcc
      241 cggatcaggg cgtccacaac gcggtccacg ccgcgcacca gaaccagatc gtcttcaccc
      301 ggatcgacac cactgtcggc gggatcaccg aggccgacat cgtccgcgat ttcacgctgg
      361 gccagccgat gttctttcgg gtctacatgg agcgctcggc ggtcaacgcg atcgccgcgg
      421 ccaccggaat gccggccagt gcggtctatg ccgacggcgt ccactacacc gcccgcttca
      481 ccatcgatgg ccagaccttc gacacgacga tgtttccctg gggcaatccg cgcgatcatc
      541 agacctggac cacctggcgg ggccagttca tcaatcccgg cactgcccag cggacgcccg
      601 gaagcgacgc gttcttcgaa atgctgtccc gtgccacttc ggcggggctg ctcaagccgg
      661 gcaagcacac gatcaagatg gagctgatcg ccaagaccaa caccgaaaag gccggtccga
      721 tctcggctgg agtggtcgca agcgggacgt tcaatctcac ggttcccgcc ggcatcttca
      781 gccccagcaa tacgaccatt tgcggagccc ggcgcggcgg cgcgggcagc gccgcgatgg
      841 aggcccgcgc cttgtccgag gccaagcgaa tctcgcgcaa cccggaactg gtccccgtgc
      901 gggcgatcgg cattggcgag agctgggatg tcgagcgcaa cgcggtcacc ggcattccga
      961 tcgagcgcga gacctatgtg ctgatccagt cccgcggacc gaagttctgc accagcaaca
     1021 cccacagctt ctacgaagac tacatgggcg gcggttcgtt ctcgaccgcg acgggcagca
     1081 tctcggtcaa catgcgggtc ggctatgtcc cgtgtggctg cctgccctga accggtgacc
     1141 cgcacctttc acgcgccccg gccggtccgc cgcccctatt cgccgctgtt catggctctg
     1201 cggcaggcct gctccaccct gatcgagcag atggagcgca agggcgccgt cgcgctcccg
     1261 ccgctcgacc gcccgtttca gggcgaggag tgggaggcct atcaccccgg cctgatcctg
     1321 gcgacggtgg actgggacgt gccccggttg gtgttcaact gggcggtcga tgacgaccgg
     1381 ccggtccgcg ccgcccgctc gcccgattac accatcgatc ttgccgcgct caaaagcgcc
     1441 gcccgggccg atcccgatcc gcgcggatgg ttcgagagcc aggcggccta tgccctgtcg
     1501 ctggcccaga attacctgat cgacttcttt cgcgacggcc cgaccgacga catcccgttc
     1561 gaacggcacc tgatcttcgt gcgcgggctg gagggcctgg acggcaccct gtggatcaag
     1621 aacgtgctgt tcgcctttga agagaccccc ggctgaaaag gaaccgccat tatctggaag
     1681 aaggcgcgcg aaagcgatgc gcacgacagt gtgctggtct gcaacgaaac ccattttcag
     1741 gggatcgccc ccgcaatccg cgcgctggtc gagcggatgg aggccgattg gcttgcgctt
     1801 ggctttgcga aggaggacgc ggtcttcgtc gatgtcaacg ccaccaacgg ccacttgatg
     1861 ctgacctggg acaagtcagg cggaggcggc tctttctatg acgagaactg gccggtctat
     1921 tatcttgagc tgaaggcgtt gtggcgcgaa tcgctcgacc atccggaagg cgcctatcac
     1981 tttgacaacc acgcccacat cgcgctgtgc agtgtcgtcg aggccttgct gatcgaggcg
     2041 gaggccgccg atcaacccga gcggtacgaa ttctacgaac tgttcgaggg cagcacgaac
     2101 ggcgcggtgc gggttctggt gtgaccggta cggtggcgat gcgcagccag gcgaccggct
     2161 ggcgcggcct ggccgatgac ctgctccgcg cgatcgactg gctcgatgcc cacggcgcag
     2221 ccggggagcc cgccatccga atcaccgtgg cggcggaccg gcgcagcctg agcctgtgcg
     2281 cctgcgacgc gcccggcgcg cccgtcctga cccttgccct gggagtgctg ccggaaacga
     2341 cggataccgc gcgcgaggac ggccagaggc cgcccaatgc cgccatcgac cgcgcggtgt
     2401 gctttgccgc ggcggccatg ctcagctggt caaaggaccc ggagaccttc gaactgctga
     2461 cccaccagcg ccacgccctg ctgctggcgt gggacgacgg gccggcaagt ccggtcgatc
     2521 cctgggccta tggcctgtgg gacggctgac ggggaccctg caggggaggg cccgggcacc
     2581 gcgcgcgcat tgggggaagg ggaatggtgc tgctggggag gattgaactc ccgacctcag
     2641 ccttaccaag gatgcgctct accactgagc tacagcagca caccattccg cttggggtcc
     2701 gggtgcggcg gggccgcccg gacaggggcg cgctattggc ggtgcgccct tgctttgtca
     2761 agccgaaccc tgatagcgca gagagattgc cgatgcgtga agaccaaccg cccctgaccc
     2821 gcgaggaacg cctggccgcc aagctgcgcg aaaacctgcg ccgccgcaag gaacaggcgc
     2881 gcgcactggg tgcgcaggtc cttcccaaag acgacccgac ccgctagggg ccagcctcgc
     2941 gctggttgcc agcctttcaa tccctgaagg agcccgcctt gccccgcttg atccttgttc
     3001 gccatggcca gagccagtgg aacctggaga accgcttcac cggctggtgg gacgtggacc
     3061 tgaccgccaa gggcgaggac gaggcccgcg ccgccggggc gctgctcaag gccaagggca
     3121 tgctgccgac cgtggccttc acatcgctgc agacccgcgc aatccgcacc ctgcacttcg
     3181 cgctggaaag cgccgggcgg ctgtggatcc cggaaaccaa ggactggcgc ctgaacgagc
     3241 ggcactatgg cggcctgacc gggctcgaca aggccgagac cgccgccaag cacggcgacg
     3301 agcaggtcaa gatctggcgc cgcagcttcg ataccccgcc tccggtgctg gaagacggca
     3361 gcgaattcga tctcaaggcc gatccgcgtt atgccgggat cgcgatcccg tcgaccgaaa
     3421 gcctgaagga cacgatcgcc cgggtcctgc cctattacga ggcggaaatc gccccggcgc
     3481 tgaaggccgg cgaagtggtg ctggttgccg cccacggcaa ttcgctgcgg gccctggtca
     3541 agcacctgtc gggcatttcc gatgccgaga tcaccgggct ggaaatcccc accggccagc
     3601 cgatcgtcta cgagctggac gacacccttg cagcgcgcga gcgctattac ctggcggagc
     3661 ggtaggatga acgcagccgt tgcgatcgtg atgggcagcc agtcggactg ggcgacgatg
     3721 aagtgcgccg ccgacgcgct cgacaagctg ggggtgtcct atgatgcccg gatcgtctcg
     3781 gcccaccgca ctccggaacg gctggtcaag ttcgcgcaag gggcggccga cgaaggcttc
     3841 aaggtgatca tcgccggggc cggcggcgcc gcgcacctgc ccggcatggt cgccagcatg
     3901 acccacctgc cggtgctggg cgtgccggtc cagtccaagg cgctgtccgg gcaggacagc
     3961 ctgctgtcga tcgtgcagat gccggccggg atcccggtcg gaaccctggc catcggcgag
     4021 cccggggcca ccaacgccgg gctgctggcc gcggcgatcc tggccaccac cgatccggcc
     4081 ctggccgagc ggatcaaggc cttccgcgcc gcgcaaagcg ccggcgtggc cgagcggccc
     4141 agctgagggc ccgcggcacg ccatgatcgc acccggcgaa acgatcggca tcctgggcgg
     4201 cgggcagctt ggccgcatgc tggccaaggc cgcggcgcag ctgggctacc gcgtccacgt
     4261 ctatgcgccg gaagccgatt cggtcgcggc cgaggtctgc gcccagttca cctgcgcggc
     4321 ctgggacgat gcccgggcca tggccacttt cgccgccgac tgcgcggtgg tgacctacga
     4381 gttcgagaac gtccccgtcg gcccgctgaa ggcgctgggc agcgtgccgc tgcaccccaa
     4441 cgcccgtgcg ctggaagtgg cgcaggaccg gctgaacgag aaggaattcg tcgagcagct
     4501 gggcggccgc accgcgccct atatggcggt cgacacccgc gaggatctgg acgaggcgct
     4561 ggtccggatc ggatcgcccg ggatcctcaa gacccggcgc gagggctatg acggcaaggg
     4621 ccaatggcgg atcaattacg accacgaggc cgagggaatc gcgctgggcg cccagccgct
     4681 gatcttcgag gggctggtcc gcttcagcgc cgaattcagt gtgatcctgg cccgcgggca
     4741 ggacggcgcg gtggtgttct gggacagcgc cgagaacgtc cacgaacacg gcctcctggc
     4801 ccgctcgacc gcgccggcca gcccggcggt ccaggcccag gtcgaggcgg ccagggccct
     4861 ggcgaagaaa gtggccgacg ggctcggcta tgtcggggtg gcggcgttcg aattcttcgc
     4921 gaccgaggac ggcccggtgt tcaacgaaat ggccccgcgg gtccacaatt cgggtcactg
     4981 gacgatcgag ggcgcggtca ccagccagtt cgagaatcac atccgcgcga tctgcggcct
     5041 gccgctgggc gacacccgcc ttgccgccgc acgggtcgag atgcgcaaca tcatcggcga
     5101 tgcggccgag gactgggcgg cggttctggc cgatccggcc aaccacctgc acctctacgg
     5161 caagggtgcg gcgcggcccg ggcgcaagat gggccatatc acccggctga ccttctagat
     5221 ggaccggcca gagatcgtc
//
LOCUS       DASUDV010000337         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1115556,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000337 DASUDV010000000
VERSION     DASUDV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115556"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..997
                     /locus_tag="VFV30_07385"
     CDS             35..997
                     /locus_tag="VFV30_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX4847950.1"
                     /translation="MSEEFAVEAELPLESVGTRLARAREAAGLSRAQIAATTRIPERH
                     LAAIETGDFAALPARTYAVGFARSYAKALGLDDKAIVEEVRAELADQAIEPPRRGVPT
                     FEPGDPARVPSARLAWLSALGVLIAVLIGYLAWPSLFAPGGTLPSILPKEAPSPAAPA
                     PAPSQAAPTGPVVFTAMEPDLWVKFYDGAGNQLLQKQLAQGETWTVPADQPQVLIWTA
                     RPDALAITIGGQAVPRLSDQQKTIKDVPVTAAALLARADPAAQSPAPVTTPAAGTTPS
                     APRRTTPQRNRQPAAALPSPEPAAAPTPAAAAAEAGSAPQPSAT"
     gene            1146..>2038
                     /locus_tag="VFV30_07390"
     CDS             1146..>2038
                     /locus_tag="VFV30_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847951.1"
                     /translation="MIKTLRKRPGFLAALALVMAAPALAQNAADPAAEARLRKLEAEV
                     AALQRKVFPGGDGTFFPQLQPGQAATTPAGTPATGPVTDILARMDALEAQIARLTAQS
                     EEGANKLGKLETRVTALESAGSAATPPAATTTPAPAPATTAPAPVATTPKPATSTPVV
                     ATPAPKPATTAPKPAATTPAVTTPKPAAAAPSATRLAAVRAIEKPQTADPGDDEYSYG
                     FRLWEAKFYPESQQQLKLYLQKYPKHSRVSFARNLLGRAYLDEGNLDEAGKWFYENYK
                     ADKTGARAPDSVLYLADTWLR"
BASE COUNT          347 a          771 c          640 g          280 t
ORIGIN      
        1 agcaagccaa taatttcaac aagacggggt cgtaatgtcg gaggaatttg cggtggaggc
       61 tgaactgcct ctcgaaagcg taggaacgcg cctggcgcgg gcccgcgagg ctgccggact
      121 ttcgcgtgcg cagatcgccg ctaccacccg cattcccgaa cgccacctcg ccgcgatcga
      181 gactggcgat ttcgcggcgc ttccggctcg gacctacgcg gtcggcttcg cccggtccta
      241 tgccaaggcg ctgggcctcg atgacaaggc gatcgtcgag gaagtccgtg ccgagctggc
      301 cgaccaggcg atcgaaccgc cgcgccgcgg ggtgccgacc ttcgaacccg gtgatccggc
      361 ccgcgtgccc agtgcccggc tggcctggct ttcggcgctg ggcgtgctga tcgcggtgct
      421 gatcggctat cttgcctggc ccagcctgtt cgcgccgggc ggcacattgc cctcgatcct
      481 gccgaaggaa gcgcccagcc ccgctgcacc cgctccggcg ccgagtcagg ccgccccgac
      541 cggcccggtg gtcttcaccg cgatggaacc tgatctgtgg gtcaagttct atgacggcgc
      601 gggcaaccag ctgctgcaga aacagctggc ccagggcgaa acctggaccg ttccggcaga
      661 tcaaccgcag gtcctgatct ggaccgcgcg gcccgatgcc ctggctatca ccatcggcgg
      721 gcaggccgtg ccccggcttt cagaccagca gaagaccatc aaggacgtgc cggtaaccgc
      781 cgccgcattg ctggcccggg ccgatccggc ggcccagtcg cccgccccgg tcacaacccc
      841 cgccgccggc accacgcctt ccgcgccgcg ccgcaccacg ccgcagcgca atcgccagcc
      901 cgctgccgcg ctgcctagcc ccgaaccggc agctgcgccc acgccggctg ccgccgctgc
      961 agaagccggg tcggctccgc aacccagcgc cacctagcct gcggatagtt gcgccggctc
     1021 gctcgacagc ccgcgcgccg cgggtttaag caggtgcaaa cggttcatcg gcggttcggc
     1081 aatatcggtg catggccatc cgccagccag acaagacagg caccagccac ggaattgaac
     1141 gaacgatgat caagaccctt cgcaagcgcc ccggtttcct tgccgccctg gcgctggtca
     1201 tggccgctcc ggccctcgcg cagaacgctg ccgatccggc cgccgaagcc cgcctgcgca
     1261 agcttgaggc agaagtcgct gcgttgcagc gcaaggtctt ccccggtggt gatggcacct
     1321 tcttcccgca gctccagccg ggccaggccg ccaccacccc tgcgggtacc ccggccaccg
     1381 gcccggttac cgatatcctg gcgcggatgg acgcgctcga agcgcagatc gcccggctga
     1441 ccgcgcaaag cgaagagggc gcaaacaagc tgggcaagct ggaaacccgg gtgaccgcgc
     1501 tggaaagcgc aggcagcgcg gcgaccccgc cggcagcaac aaccaccccc gcgcccgcac
     1561 cggcaaccac cgctccggcc ccggtagcca ccacgcccaa gccggccacc agcaccccgg
     1621 ttgttgcgac cccggcgccc aagccggcga ccaccgcgcc taagccggcc gccaccacgc
     1681 cggcggtcac tacgcccaag cccgccgccg ccgcgcccag tgccacccgg ctcgccgcgg
     1741 tccgggcgat cgaaaagccg cagaccgccg atccgggcga tgatgaatat tcctatggct
     1801 tccgcctgtg ggaggccaag ttctatccgg aatcgcagca gcagctgaag ctttacctgc
     1861 agaagtaccc caagcattcg cgggtcagct ttgcgcgcaa cctgctgggc cgggcctatc
     1921 tggacgaggg caaccttgat gaggcgggca agtggttcta cgagaactac aaggctgaca
     1981 agaccggcgc ccgcgccccg gattcggtac tctatctggc cgatacctgg ctgcgcaa
//
LOCUS       DASUDV010000338         8259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_519385,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000338 DASUDV010000000
VERSION     DASUDV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519385"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /locus_tag="VFV30_07395"
     CDS             <1..1966
                     /locus_tag="VFV30_07395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4847952.1"
                     /translation="DGAVVVWQQTETDPASYKRSTGLWRVPAKGGTPERVADLPEAGE
                     NSPAFSPDGKRLYFLSDKSGKDQVWFLDLTAPGAAPVQASDFKTDVAGFELSPDGKRI
                     LVWGDVARDCPTLGCEASGDTSQPGPGTGRHYKHGTGFVRHWDAWETAGNYSRAFALD
                     LGADGKVAGTAVALDGPPGVLTGDTPSKPMGGGEELAWAADSKSVFFTARQADAKEPL
                     STNLDVWHAMLDGKAPHSPTKANLATDTMPTPSPDGKWLAWAAMERPGYESDRLVIHV
                     MNLATHEKRVLTGAWDRSASSLAWTPDSKALIVTAEEVLDTPAFRIDLASGKVTRLAL
                     APKGLTEGHIGNVTPLPGGALLYTRDSVSGPAEVWIAEKGKAPRQLSRANDAQLAQLV
                     PLKSERFSFKGAGGATVWGQIHTPANATGKLPVLLFVHGGPQGSFGDGWSFRWNPRVF
                     AAQGYAVVSIDFHGSTGYGQAFTDSINQDWGGKPLEDLKLGLDAALAGRPQLDGSRAC
                     ALGASYGGYMMNWIEGQWPDRFKCLVNHNGVFDSRGMAYGTEELWFNEWEHGGKPYHE
                     TPEAYEKWNPANHVAKWQTPMLVVIGEKDFRIPYSQGLSAFTALQRKGIEAELLVFPD
                     ENHWVLKPKNSLQWHRTVFTWLDRWLKPGE"
     gene            1971..2402
                     /locus_tag="VFV30_07400"
     CDS             1971..2402
                     /locus_tag="VFV30_07400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4847953.1"
                     /translation="MARPDAALLDPARYPFAHTITTRFADIDPNQHLNNVALAAMMED
                     ARVRFNMSLGSAIRIGERRAMVASVAMEYLSQGHFPDPVTVHCAVEKVGNSSWSVVQL
                     LVQNGRPVAFARSALVAIADDRPTPIAEEYRAVLSAWSLRA"
     gene            2399..2785
                     /locus_tag="VFV30_07405"
     CDS             2399..2785
                     /locus_tag="VFV30_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX4847954.1"
                     /translation="MSGDSAELERARAALATNGGDKTRRHIFLCAEAQKGECCAAEVG
                     GPAWKFLKKRLKELKLDRDGGIARTKADCLKICAAGPIAVVYPEGVWYHSATEPVLER
                     IIQEHLVGGVPVEDFRLHPADNDLSA"
     gene            2887..3315
                     /locus_tag="VFV30_07410"
     CDS             2887..3315
                     /locus_tag="VFV30_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4847955.1"
                     /translation="MTIARLIEGRSGDIVSCDTRTSVAEAVKILAEKRIGALPVFAGG
                     KIAGIFSERDVVYQLAAHGPAMLEKTVGEVMTSPAITVDPATDVLDALGQMTRRRIRH
                     LPVVEGEAVTGFVSIGDLVKYRIDRIVSEAEAMRSYIQSA"
     gene            3355..3687
                     /gene="erpA"
                     /locus_tag="VFV30_07415"
     CDS             3355..3687
                     /gene="erpA"
                     /locus_tag="VFV30_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX4847956.1"
                     /translation="MDVSTMTLSPSAAARVAAIAAKQGKPAILRLAVEGGGCSGFQYK
                     FALEETPDAEDLVVERDGVRLVVDPVSLDLLAGSEVDYVESLGGAAFRVTNPNASAGC
                     GCGSSFAV"
     gene            3687..4466
                     /gene="xth"
                     /locus_tag="VFV30_07420"
     CDS             3687..4466
                     /gene="xth"
                     /locus_tag="VFV30_07420"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX4847957.1"
                     /translation="MRIATFNINGIKARLPRLLEWLAETRPAVACLQEIKTQDEGFPA
                     EEFEKLGYHALWHGQKSFNGVAILADGTAPVEIQRGLPGDPADEQSRYLEAEVFGTRV
                     ACIYLPNGNPLPGPKFDYKLAWMERLRARMDALRAEEVPAVVLGDFNVIPHDHDVWSP
                     KAMADDALMQPESRNAYFRLLHGGWTDALAAHNPQGGVWTYWDYQAGAWQRDHGFRID
                     HALLSPELADRLVACGVDKEHRGREKASDHAPVWIEIAPAG"
     gene            complement(4519..5166)
                     /locus_tag="VFV30_07425"
     CDS             complement(4519..5166)
                     /locus_tag="VFV30_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX4847958.1"
                     /translation="MSRKLHQASMISVAAMIAFVIASAGGSGAAAQDRTPAPVIQEPA
                     IQTISQPVVQPLPATAETPAVDASEPLPEARPVKAASLAAMVAAQPQPSDLPRELFCL
                     AGATYFEAKSESLAGQLAVARVVVARSKSGRFPNSYCGVVFQPSQFSFVRGGSMPRIA
                     KGSKQWKNAVAIAQIAHSNAWASPVEGALFFHAAYVSPGWKLKRVGRVDNHVFYR"
     gene            complement(5363..5704)
                     /locus_tag="VFV30_07430"
     CDS             complement(5363..5704)
                     /locus_tag="VFV30_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX4847959.1"
                     /translation="MSARLPAHLEVAGLIRAVEAAGGFAAVVRKGEREAGTILVILTE
                     KGANARLFERMPDPEGGRIWHCTAIQDTENKPEFNDQLARRASRDPDLWIVELDIADG
                     ERFIGLTGSVG"
     gene            complement(5701..6162)
                     /locus_tag="VFV30_07435"
     CDS             complement(5701..6162)
                     /locus_tag="VFV30_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX4847960.1"
                     /translation="MTALFTLMPEAVGTIDADSKPAILDQLAQRFAGVYGLDPALVLE
                     RIEEREKLGSTGFGRGVAIPHARIPGLGRPVGAFLRLTTPVAFDAADGMPVDLVFGLL
                     SPEAAGAAHLHALAAISRMMRDEAMHRALGDAPGAEALYALLAHSIEKDAA"
     gene            complement(6278..6844)
                     /gene="raiA"
                     /locus_tag="VFV30_07440"
     CDS             complement(6278..6844)
                     /gene="raiA"
                     /locus_tag="VFV30_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX4847961.1"
                     /translation="MDIRVSGHQIETGEALQVRAQERLTAIVDKYFPKALSSSVTFNR
                     APAAAFRCDIVTHAKQGVVLKGAGIAHDAHVALDQAIEKIDKQLRRYKRRLKDHHEAS
                     AHAARAEEAAYTVFVEPDIEAEEVPAEAPLVIAETRVDVPEATVADAVMMLDLRNTNA
                     LLFKNAGTGRHNMVYRRGDGSIGWVEPS"
     gene            complement(6882..7568)
                     /gene="dnaQ"
                     /locus_tag="VFV30_07445"
     CDS             complement(6882..7568)
                     /gene="dnaQ"
                     /locus_tag="VFV30_07445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX4847962.1"
                     /translation="MREIIFDTETTGLDPKSGDRLVEIGCIEMVNRVVTGNTFHCYFN
                     PDRDMPAAAEAVHGLSIAFLSDKPRFHEKAAEFLEFIGEEAKLVAHNAGFDFGFVNNE
                     LEMCGLPPLSRDRMVDTVAMARVRHPGAKNSLDALCTRYGIDRSHRTRHGALLDAELL
                     SQVYVELTGGRQIGLSLAAESNVVAITTLSAPRRERPFRPARPHAASEEEIAAHAAFL
                     ETVKSPLWRD"
     gene            complement(7619..8218)
                     /gene="coaE"
                     /locus_tag="VFV30_07450"
     CDS             complement(7619..8218)
                     /gene="coaE"
                     /locus_tag="VFV30_07450"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443777.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX4847963.1"
                     /translation="MSKPFILGLTGSIGMGKSAVAAMFRGLGVPVFDADAAVHELQGP
                     GGACVAPIERAFPGTTGPGGVDRQKLGAAVFGNPEALKRLEQIVHPEVAEMRRAFLAD
                     NAAAPLIVFDIPLLYEKTGQHGLDAVVVVSAPAAVQRERVLARPGMTPEKFEQILKLQ
                     VPDAEKRARADHVIDTGVSLAETRHAVARLVHAITGQEK"
BASE COUNT         1383 a         2757 c         2797 g         1322 t
ORIGIN      
        1 cgacggggcc gtggtggtgt ggcaacagac cgaaaccgat ccggccagct acaagcgcag
       61 caccggcctg tggcgcgttc cggccaaggg cggcacgccc gagcgcgtgg ccgacctgcc
      121 cgaggccggt gagaacagcc cggccttcag ccccgacggc aagcggctct atttcctctc
      181 ggacaagagc ggcaaggacc aggtctggtt cctcgatctg accgcgcccg gcgccgcgcc
      241 ggtccaggcc agcgatttca agaccgacgt ggccggcttt gaactgtcgc ccgacggcaa
      301 gcggatcctg gtctggggcg atgtcgcgcg cgattgcccg accctgggct gcgaggcgag
      361 cggcgacacc agccagccgg gtcccggcac cggacggcac tacaagcacg gcaccggctt
      421 tgtccgccac tgggatgcct gggaaacggc gggcaactac agccgcgcct tcgcgctcga
      481 tctcggcgcc gacggcaagg tggcgggaac cgccgtggcg ctcgacggcc ctcccggggt
      541 gctgaccggc gacaccccgt ccaagccgat gggcggcggc gaggagttgg cctgggcggc
      601 ggattcgaag agtgtgttct tcaccgcccg tcaggccgac gcgaaggagc cgctgtcgac
      661 caacctggat gtctggcacg ccatgctgga cggcaaggcc ccgcacagcc cgaccaaggc
      721 caacctggcg accgacacca tgcccacccc gtcgccggac gggaaatggc tggcctgggc
      781 ggcgatggag cggcccggct atgaaagcga ccggctggtg atccacgtga tgaacctggc
      841 gacccacgag aagcgggtgc tgaccggggc gtgggaccgg tccgccagct cgctggcctg
      901 gaccccggat tccaaggcgc tgatcgtcac cgccgaagag gtgctcgaca ccccggcctt
      961 ccggatcgac ctcgccagcg gcaaggtcac ccggctggcg ctggcgccca aggggctgac
     1021 cgagggccat atcggcaatg tcaccccgct gcccggcggg gcgctgctct acacccgcga
     1081 cagcgtctcg ggcccggccg aggtctggat cgccgaaaag ggcaaggccc cccgccagct
     1141 gagccgcgcc aacgatgccc aactggccca gctggtcccg ctgaagagcg agcggttcag
     1201 cttcaagggc gcgggcggcg ccaccgtctg gggccagatc cacacccccg ccaatgccac
     1261 cggcaagctg ccggtcctgc tgttcgtcca cggcggcccc cagggcagct ttggcgacgg
     1321 ctggtcgttc cgctggaacc cgcgggtctt tgccgcccag ggctatgccg tggtctcgat
     1381 cgatttccac ggcagcaccg gttacggcca ggcctttacc gatagcatca accaggactg
     1441 gggcggcaag ccgctggagg atctcaagct cggcctcgat gccgcgctgg ccggccgccc
     1501 gcagctggac gggtcgcggg cctgcgcgct gggcgcgtcc tatggcggct acatgatgaa
     1561 ctggatcgaa ggccagtggc ccgatcggtt caagtgcctg gtcaaccaca acggcgtctt
     1621 cgattcgcgc ggcatggcct atggcaccga agagctgtgg ttcaacgagt gggagcacgg
     1681 cggcaaaccc tatcacgaga cgcccgaggc ctatgagaag tggaacccgg ccaaccacgt
     1741 ggccaagtgg cagaccccga tgctggtggt gattggcgag aaggacttcc gcatccccta
     1801 ttcgcagggg ctcagcgcct tcaccgcgct gcagcgcaag gggatcgagg ccgagctgct
     1861 ggtcttcccg gacgagaatc actgggtgct caagcccaag aactcgctgc agtggcaccg
     1921 cacggtcttc acctggctcg accgctggct gaagccgggc gaataaggac atggcccgcc
     1981 ccgacgccgc gctgctcgat cctgcgcgct atccctttgc ccacacgatc accacccggt
     2041 tcgcggacat cgaccccaac cagcacctca acaacgtggc gctggcggcg atgatggaag
     2101 acgcccgggt gcgcttcaac atgagcctgg gcagcgcgat ccggatcggg gagcgccggg
     2161 cgatggtggc tagcgtggcg atggaatacc tctcgcaagg acacttcccc gatccggtca
     2221 cggtccactg cgcggtcgag aaggtcggca attcgagctg gagcgtggtg cagctgctgg
     2281 tgcagaacgg tcgacccgtg gccttcgcgc gctcggccct ggtggcgatt gccgacgacc
     2341 ggccgacccc gatcgccgag gagtaccgcg cggtcctgag cgcgtggagc ctgcgggcat
     2401 gagcggcgat tccgccgaac tggagcgggc ccgcgccgcg ctggccacca acggcgggga
     2461 caagacccgg cgccatatct tcctctgcgc cgaggcccag aagggcgaat gctgtgcggc
     2521 cgaagtgggc gggccggcgt ggaaattcct caagaagcgg ctcaaggagc tgaagctcga
     2581 ccgcgacggc ggcattgccc ggaccaaggc cgattgcctg aagatctgcg ccgccgggcc
     2641 gatcgcggtc gtctatcccg aaggcgtctg gtaccattcg gcgaccgagc cggtgctcga
     2701 acggatcatc caggagcacc tggtcggcgg ggttccggtt gaggacttcc ggctccatcc
     2761 cgcggataac gacctgtccg cctgatgccg tcttcggctc tgccgaagct acatccgaac
     2821 cctgactcgc caaagcgccc ggttcgtgtc atcctttcgg gatcagaaca gacccggaga
     2881 gttgccatga cgattgcgcg actgattgaa gggcgaagcg gcgacattgt cagctgcgat
     2941 acccggacaa gtgtggccga ggcggtgaag atcctggcgg aaaagcggat cggggccctg
     3001 ccggtctttg ccggaggcaa gatcgccggg atcttttccg aacgcgacgt ggtctaccag
     3061 ctggccgcgc acggcccggc gatgctggaa aagaccgtgg gcgaggtgat gacctcgccg
     3121 gcgatcaccg tcgatcccgc caccgacgta ctcgacgcgc tgggccagat gacgcgccgc
     3181 cggatccgcc atttgccggt ggtggaagga gaggccgtga ccgggtttgt ctcgatcggc
     3241 gacctggtca aataccggat cgaccggatc gtgtccgagg ccgaagcgat gcgcagctac
     3301 atccagagcg cgtgattcct tgccgctggc ccctgcaggc cctacatcgg cggcatggac
     3361 gtttcgacga tgacactcag cccctccgcc gccgcgcgcg tcgcggcgat tgcggccaag
     3421 cagggcaagc ccgccatcct gcgccttgcg gtcgagggcg gcggctgttc gggctttcag
     3481 tacaagttcg cgctggaaga gacgccggac gctgaagatc tggtggttga gcgcgacggc
     3541 gtgcgcctgg tcgtcgatcc ggtcagcctc gacctgctgg ccggcagcga ggtcgactac
     3601 gtggaatcgc tgggcggcgc ggcgttccgg gtaaccaatc ccaacgccag cgcgggctgc
     3661 ggctgcggct cgtccttcgc cgtctgatgc ggatcgccac gttcaacatc aacgggatca
     3721 aggcgcgctt gccgcggctg ctcgaatggc tggcggaaac ccgccccgcg gtcgcctgcc
     3781 tgcaggagat caagacccag gacgagggct ttccggccga ggagttcgag aagctgggct
     3841 atcacgcgct gtggcacggc cagaagagct tcaacggcgt ggcgatcctt gccgatggca
     3901 cagcaccggt cgagatccag cgcggcctgc ccggcgatcc ggccgacgag cagtcccgct
     3961 atctggaggc cgaggtgttc ggaacccggg tggcctgcat ctacctgccc aacggcaatc
     4021 cgttgccggg ccccaagttc gattacaagc tggcctggat ggaacggctg cgcgcgcgca
     4081 tggacgcgct ccgcgccgaa gaggtgccgg ccgtggtgct gggcgatttc aacgtcatcc
     4141 cgcacgatca cgatgtctgg tcacccaagg cgatggccga tgacgcgctg atgcagcccg
     4201 aatcgcgcaa tgcctatttc cgcctgctgc acggcggctg gaccgatgcc ctggccgccc
     4261 acaacccgca gggcggggtc tggacctatt gggattacca ggccggagcc tggcagcgcg
     4321 accacgggtt ccggatcgac cacgccctgc tctcgcccga actggccgac cggctggtcg
     4381 cctgcggagt cgacaaggag caccgcggcc gggaaaaggc cagcgatcac gcgccggtgt
     4441 ggatcgagat cgcgccagct ggatagagaa agggcggacc cacggcccgc ccctgtcctg
     4501 aaatccggcc tgccggactt aacgatagaa gacgtggtta tcgacgcggc cgacgcgctt
     4561 cagcttccag ccggggctga cataggccgc gtggaagaac agcgcgcctt cgaccgggct
     4621 ggcccaggcg ttgctgtgcg cgatctgggc gatcgccacg gcgttcttcc actgcttcga
     4681 gcccttggcg atccgcggca tcgagccgcc gcgcacgaag gagaactgcg agggctggaa
     4741 caccacgccg cagtaggagt tggggaagcg gcccgacttc gaacgggcca cgacgacacg
     4801 ggccacggcc agctggccgg ccagcgattc gctcttggct tcgaaatagg tcgcgccggc
     4861 gaggcagaac agctcgcgcg ggaggtccga cggctgcggc tgggccgcga ccatcgcggc
     4921 cagcgaagcg gccttgaccg ggcgggcttc gggcagcggt tccgacgcgt caaccgccgg
     4981 cgtttcggcc gtggcgggaa gcggttggac aaccggctgc gaaatggtct ggattgctgg
     5041 ttcctggatg accggagcag gagttctgtc ctgtgcggcc gcgcccgaac caccggcgct
     5101 ggcaatcacg aatgcaatca tggcggccac cgaaatcatc gaggcctggt ggagcttacg
     5161 gctcattgaa gcgtcttttt aggcggtgga cgcgcccgac gcaggatggt tgaggttgaa
     5221 actgcgagag ttcttgtgcc ccaagccgga aatcctgccg gctgcccccc gtctgcgtgc
     5281 ctgcgttgcg gaccgaaatg cccgcgcgcg ccgcctgcgc atcaaaaggt gagcgggcct
     5341 ttgattgtgc agcgcagcaa agtcaaccca ccgaacctgt caatccgatg aaccgttcac
     5401 catccgcgat atccagttcg acaatccaca gatcggggtc ccggctggcc cgccgggcca
     5461 gctgatcgtt aaattcgggt ttattttccg tatcttgaat ggcggtgcag tgccagatcc
     5521 ggccgccttc cgggtcgggc atacgctcaa aaagccgcgc gtttgcgccc ttttcggtca
     5581 ggatgacaag gattgtcccg gcttcgcgtt cacctttgcg aaccaccgcc gcaaagccgc
     5641 cggcggcctc gaccgcgcgg atcaggcccg ccacctcgag gtgggcgggc aggcgggcgc
     5701 tcaggccgcg tccttctcga tcgagtgggc cagcagcgca tagagcgcct cggccccggg
     5761 cgcgtcgccc agcgcgcggt gcatcgcttc gtcgcgcatc atccggctga tcgcggccag
     5821 cgcgtgaagg tgggccgccc cggcggcttc gggcgaaagc agcccgaaca ccaggtccac
     5881 cggcatcccg tcggccgcat cgaaggcaac cggggtggtc agccgcagaa aggcaccgac
     5941 cggccggccc aggcccggaa tccgcgcatg cggaatcgcc acgccgcggc cgaaaccggt
     6001 cgagcccagt ttctcgcgtt cttcgatgcg ttccagcacc agcgccggat cgaggccgta
     6061 aaccccggcg aatcgctgcg ccagctggtc gaggatcgcc ggcttgctat cggcatcaat
     6121 cgtgccgacc gcttcgggca taagggtaaa aagtgcagtc attacaggcg atcaatccat
     6181 cggggcacgg tcaaaggcag cctgcccgca tcatgcgggt attgccgctc caacccccct
     6241 cgtttgttcg gaattgcgcg cgcctttacc ctgcgcatca agaaggttca acccacccga
     6301 tcgatccatc cccgcggcgg tagaccatgt tgtgccgccc ggtgcccgcg ttcttgaaca
     6361 aaagcgcgtt ggtgttgcgc aggtccagca tcatcaccgc gtcggccacg gtcgcttcgg
     6421 gcacgtcgac ccgggtttcg gcaatcacca gcggcgcttc ggccgggact tcctcggcct
     6481 cgatatcggg ttcgacgaag acggtatagg cggcctcttc ggcccgcgcg gcatgggccg
     6541 aggcttcgtg gtgatctttc agccggcgct tgtagcggcg cagctgcttg tcgatcttct
     6601 cgatcgcctg gtccagcgcg acatgggcat cgtgcgcgat cccggcgccc ttcaagacca
     6661 cgccctgctt ggcatgagtc acgatgtcgc agcgaaaggc cgcggccggc gcgcggttga
     6721 acgtgaccga actggacagg gccttgggga agtacttgtc gacaatcgcg gtcaggcgtt
     6781 cctgcgcgcg cacctgcagt gcttcgccgg tttcgatctg gtggccggat acgcgaatgt
     6841 ccatggtggt cttctcctgt cctaaacggc cccgaaccgg atcagtcgcg ccacagcgga
     6901 gacttcaccg tttcgaggaa tgcggcatgg gccgcgatct cctcctcgct cgccgcatgc
     6961 ggacgggcgg ggcggaaggg gcgttcgcgc cggggcgcgg aaagggtggt gatcgcaacg
     7021 acgttgcttt cggcggccag tgaaaggccg atctggcggc cgccggtcag ctcgacatag
     7081 acctgggaga gcagctcggc gtcgagcagc gcgccgtggc gggtgcggtg gctgcggtcg
     7141 atcccatagc gggtgcagag cgcgtcgagc gagttcttgg cgcccgggtg gcggacccgc
     7201 gccatcgcca cggtatcgac catccggtcg cggctgagcg gcggcaggcc gcacatctcc
     7261 agctcgttgt tgacgaagcc gaagtcgaac ccggcgttgt gcgcgaccag cttggcttcc
     7321 tcgccgatga attcgaggaa ttcggcggcc ttttcgtgaa accgcggctt gtccgaaagg
     7381 aatgcgatcg acagcccgtg caccgcttcg gccgcggccg gcatgtcccg atcggggttg
     7441 aaatagcagt gaaaggtgtt gccggtgacg acccggttga ccatctcgat gcagccgatt
     7501 tcgaccagcc ggtcaccgct cttgggatcg agaccggtgg tttccgtatc gaagataatc
     7561 tcgcgcattg cccaaccgat atctgcctgt tggcgcaggg atacaagggg gtaggcggct
     7621 atttttcctg tccggttatc gcatggacca ggcgcgccac cgcatggcgg gtttcggcca
     7681 ggctgacccc ggtatcaatc acgtgatcgg cccgggcgcg cttctccgcg tcggggacct
     7741 gcagcttcag gatctgctcg aacttttccg gggtcatccc cggccgggca aggacccgct
     7801 cgcgctggac cgcggccggc gcggagacca ccaccacggc atcgagcccg tgctgcccgg
     7861 tcttttcgta gagcagcgga atgtcgaaca cgatcagcgg cgcggcggcg ttgtcggcca
     7921 gaaaggcccg gcgcatttcg gccacttcgg ggtgaacgat ctgttccagc cgcttcagcg
     7981 cctcggggtt gccgaacacc gccgcgccca gtttctggcg gtccacgccg ccgggaccgg
     8041 tggtgccggg aaaggcccgc tcgatcgggg ccacgcatgc cccgcccgga ccctgcaatt
     8101 cgtgcaccgc ggcatccgca tcgaacaccg gcacgcccag cccgcgaaac atcgccgcga
     8161 ccgcgctttt gcccatgccg atcgagccgg tcaggccaag gatgaaaggc ttgctcatcg
     8221 cgtcaggatc ccgcgcagct cggcatcgtg cccgcgcgg
//
LOCUS       DASUDV010000339         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_739823,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000339 DASUDV010000000
VERSION     DASUDV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739823"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFV30_07455"
     CDS             complement(<1..542)
                     /locus_tag="VFV30_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX4847964.1"
                     /translation="MSLPNTMPALVKARPEPGIWLEQQPVPEPGPDEVLIRVHRTAIC
                     GTDIHIYNWDDWAQRTIPVPMIVGHEFGGEIAAIGSNVKRPLSIGQRVSGEGHIIGEK
                     SRAVRAGHFHLDPETQGVGVNRPGAFAQYLCIPAFNVIALPDHVDMDVAAMLDPLGNA
                     VHTALAFDLIGEDVLITGAGP"
     gene            complement(544..1746)
                     /locus_tag="VFV30_07460"
     CDS             complement(544..1746)
                     /locus_tag="VFV30_07460"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX4847965.1"
                     /translation="MRDAFYAQVRETLAEIESQGLTKHERQLTSAQAVEVEVATPAGT
                     RRVINFCANNYLGLADHPDLVAAAHATSASHGFGMASVRFICGTQDIHRQLERRIAAF
                     FGYEDSITFAACFDANGAVFEPLFGEDDAIISDSLNHASIIDGVRLSKAKRFRFANND
                     MADLEVQLQAADAAGARSKIIVTDGVFSMDGFIANLGAICDLADRYGALVMVDDCHAA
                     GFMGEGGRGSIEHCGVMGRVDILTGTLGKALGGGMGGYICARQEVVDLLRQRARPYLF
                     SNALAPGLVGASLKVFDLLEAGNPFRAQLFANAARFRAGMAEAGFTLQPGEHPIIPVM
                     LGDARLAQQMAARLLDEGIYVTGFSFPVVPRGQARIRTQMSAAHTFDHIDRAIAAFTK
                     VGRELGVI"
     gene            complement(1869..>2461)
                     /locus_tag="VFV30_07465"
     CDS             complement(1869..>2461)
                     /locus_tag="VFV30_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4847966.1"
                     /translation="VDGTDPQQLVRATQEGRRRCFALASFLVDRVPGFAKARLSGLGP
                     TVGVRETRRLEAMHRIGADELAAGAKFPDGIVCCDNPIDDVMRGDSAMTHDAAVADGD
                     FYTVPFRALVPRSVENLLFAGRLVCADPAAFASVRGMPQCMAMGEATGVAAAMALADG
                     VSVQSLDPARVVAALAARGLRGIGGENLLEQPRAVG"
BASE COUNT          416 a          840 c          823 g          382 t
ORIGIN      
        1 ggcccggcgc cggtgatcag cacgtcctcg ccgatcaggt caaaggccag cgcggtgtgg
       61 accgcgttgc ccagcggatc gagcatggcc gccacgtcca tgtcgacatg gtccggcaag
      121 gcgatgacgt tgaaggccgg gatgcacagg tactgggcaa aggcaccggg gcggttgacg
      181 cccacgccct gcgtttccgg atcgaggtga aaatgcccgg cgcgcaccgc ccggctcttc
      241 tcgccgatga tgtgcccctc gcccgaaacg cgctgcccga tcgaaagcgg acgcttcacg
      301 ttgctgccga tcgccgcgat ctcgccgccg aattcgtggc cgacgatcat cggcaccgga
      361 atggtccgct gggcccagtc gtcccagttg tagatgtgga tgtcggtccc gcagatcgcc
      421 gtgcggtgaa cgcggatcag gacctcgtcc ggaccgggtt cgggcaccgg ctgctgttcc
      481 agccagatgc cgggttcggg cctggccttg accagcgcgg gcatggtgtt gggcagggac
      541 atgtcagatt actcccagtt cgcggccgac cttggtaaag gcggcaatcg cgcggtcgat
      601 gtgatcaaag gtgtgagcgg cgctcatctg ggtgcggatc cgggcctgcc cgcgcggcac
      661 caccgggaag gaaaacccgg tgacatagat cccttcgtcg agcagcctgg ccgccatctg
      721 ctgggccagt cgggcatcac ccagcatcac cgggatgatc ggatgctcgc ccggttgcag
      781 ggtgaagccg gcctcggcca ttccggcgcg gaaccgggcg gcgttggcga acagctgggc
      841 ccggaacggg ttgccggcct ccagcagatc gaacaccttc aggctggcgc cgaccagccc
      901 cggggccagg gcgttggaaa acagataggg ccgcgcgcgc tggcgcagca gatcgaccac
      961 ctcctgccgg gcgcagatat agccgcccat cccgccgccc agcgccttgc ccagcgtgcc
     1021 ggtcaggatg tcgactcggc ccatcacacc gcaatgctcg atcgagccgc gaccgccctc
     1081 gcccatgaag ccggcggcgt ggcaatcgtc gaccatgacg agcgcgccgt accggtcggc
     1141 cagatcgcag atcgcgccca gattggcgat gaagccgtcc atcgagaaga ccccgtcggt
     1201 gacgatgatc ttgctgcgcg caccggcggc atcggccgcc tgcagctgca cttccagatc
     1261 ggccatgtcg ttgttggcga accggaaccg cttggccttc gacaggcgca ccccgtcgat
     1321 gatcgaggcg tggttgagcg aatccgagat gatcgcgtca tcctcgccga acagcggctc
     1381 gaacaccgcg ccgttggcat cgaagcaggc ggcgaaggta atgctgtcct catagccgaa
     1441 gaaggcggcg atccgccgtt ccagctgccg gtgaatgtcc tgggtgccgc agatgaaccg
     1501 gaccgaagcc atgccgaagc cgtggctggc gctggtggcg tgggccgcgg cgaccagatc
     1561 ggggtgatcg gcgaggccca ggtaattgtt ggcgcagaag ttgatcaccc ggcgcgttcc
     1621 ggcgggcgtc gccacctcca cctcgaccgc ctgagccgag gtcagctgcc gctcgtgctt
     1681 ggtcagcccc tgggattcga tctccgccag ggtctcgcga acctgggcgt aaaatgcgtc
     1741 gcgcatggct gtgcctccgt tcctgaccaa agactggcag gagaggccgg gcgcgtccag
     1801 atcatgtcta tttgatatga aggcatatcc gcaactttcg ggtcgcggtg aataattaaa
     1861 ttacggcatc atcccacggc gcgcggctgc tccagcaggt tctctccgcc aatgccccgg
     1921 aggccccgcg cggccaaggc cgccaccacc cgcgccggat cgagcgactg gacgctcacc
     1981 ccatccgcca gcgccatggc cgccgccacg ccggtcgcct cgcccatggc catgcattgc
     2041 ggcatgcccc gcaccgatgc gaaagcggcc ggatcggcgc agaccagccg cccggcgaag
     2101 agcaggttct ccacactgcg gggaacaagc gcgcggaagg ggacggtgta gaaatcgcca
     2161 tcggccacgg cggcgtcgtg ggtcatcgcc gaatcgccgc gcatgacatc gtcgatcggg
     2221 ttgtcgcagc agacgatccc gtcgggaaat ttggccccgg cagcgagttc gtccgcgccg
     2281 atccggtgca tggcttccag ccgccgggtc tcgcgcacgc ccacggtcgg ccccagtccg
     2341 ctgagccgcg ccttggcaaa gccgggcacg cgatcgacca ggaagctggc cagcgcaaag
     2401 cagcgccgcc gcccctcctg cgtggcccgg accagttgct gcggatcggt cccgtcaacg
     2461 c
//
LOCUS       DASUDV010000340         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_695736,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000340 DASUDV010000000
VERSION     DASUDV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695736"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFV30_07470"
     CDS             <1..1307
                     /locus_tag="VFV30_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4847967.1"
                     /translation="DWQEIENLGGSRIITHAEGCYINDGDGNRILDGMAGLWCVNVGY
                     GRKELADVAYEQMQELPYYNTFFKTATPPTVLLAQKIASLTQNRLPHVFFNASGSEAN
                     DTIFRLVRQYWKLKGEPQRKVFISRWNAYHGSTVAGVSLGGMKAMHPIGDLPIPGVEH
                     VRQPYWFGEGFGEDPEAFGTACAAAIEERILAVGPENVAAFIGEPVQGAGGVIIPPAN
                     YWPQVEAICRKYGILMICDEVICGFGRTGNMWGHETMGVKPDIIAMAKGLSSGYLPIS
                     AVAVSDEIIKVMKTGGDFVHGYTYSGHPVAAAVALRNIEIMEREGLVEKVRSETGTYL
                     ASALARLKDHPLVGEVRSIGLLGAVEIVAEKGTNTRWGGAEGNAGPVVRDRCIANGLM
                     VRGIRDTIVMCPPLIITPAQIDDMVAIIAKSLDEALPKLQAL"
     gene            1356..>3017
                     /locus_tag="VFV30_07475"
     CDS             1356..>3017
                     /locus_tag="VFV30_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4847968.1"
                     /translation="MRRTLLTASALIAAPGLALAQPAEEPGDAPTGEIVVTGQGLDPS
                     PLLAAYSAVAIRRDSAAPPASGRLEDVLASAAGFQQFRRSDSRSANPTAQGVTLRGLG
                     GNATSRTLVVLDGVPVTDPFFGHVPFTALPFERLERATVIRGGGSGAFGAGAVAGTLD
                     LASANPADLPLLEAALLGNDRGESELSATLAPRLGAGYAVVSGRWDRGQGFWTTPLAQ
                     RVPASARARFDSWSTSIRAVAPLAEGIELQARALVFEDRRTLRFAGADSFQGGQDLSL
                     RLVGRGAWQVDVLGYVQQRNFSNVVISATSFRRTLDQQGTPATGIGGKIELRPPVDAA
                     HSLRFGIDYRRSSGELQEVAYSGVTGAVTARRRAGGTNQDLGFYLEHDRDLGPLRLTG
                     SVRLDRWSVSDGRFRESNAAGAVVSDLAYPDRSGWDLSLRGGVLADLAPGLVLRGSVY
                     SGLRQPTLNELYRPFVVFPVTTRANAALENEDLFGFEGGLDLRLGGVTLSATLFDNRI
                     AGAIANVTIGPNLRERRNVDAVHARGLELNGRAELGRVTWDGSLSL"
BASE COUNT          445 a         1033 c         1076 g          463 t
ORIGIN      
        1 ccgactggca ggagatcgaa aacttgggcg gaagccggat catcacccat gccgagggct
       61 gctacatcaa cgacggcgac ggcaaccgca tcctcgatgg catggccggg ctgtggtgcg
      121 tcaacgtggg ctatggccgc aaggagctgg ccgacgttgc ctacgagcag atgcaggagc
      181 tgccctacta caacaccttc ttcaagaccg cgacgccgcc cacggtgctg ctcgcccaga
      241 agatcgccag cctgacccag aaccgcctgc cgcacgtgtt cttcaacgca tcgggcagcg
      301 aggcgaacga cacgatcttc cgcctggtcc gccagtactg gaagctgaag ggcgagccgc
      361 agcgcaaggt cttcatcagc cgctggaacg cctaccacgg ctcgactgtc gccggcgtct
      421 cgctgggcgg gatgaaggcc atgcacccga tcggcgatct gccgatcccg ggggtagagc
      481 acgtgcgcca gccctattgg ttcggcgagg gctttggcga ggatccggag gccttcggca
      541 ccgcctgcgc cgccgcgatc gaagagcgga tcctggcggt cggcccggaa aacgtcgcgg
      601 ccttcatcgg cgagccggtg cagggcgcgg gcggggtgat catcccgcca gccaattact
      661 ggccgcaggt cgaggcgatc tgccgcaagt acggcatcct gatgatctgt gacgaggtga
      721 tctgcggatt cggccgcacc ggcaacatgt ggggccacga aaccatgggc gtgaagcccg
      781 atatcatcgc catggcgaag ggcctgtcct ctggctacct gccgatttcg gcggtcgcgg
      841 tatcggacga gatcatcaag gtgatgaaga ccggcggcga tttcgttcac ggctatacct
      901 attcgggcca cccggtggcc gccgcggtgg cgctgcgcaa catcgagatc atggagcgcg
      961 aaggcctggt cgagaaggtc cgcagcgaaa ccgggaccta tctggccagc gcgctggccc
     1021 ggctgaagga ccacccgctg gtcggcgaag tgcgctcgat cgggctgctc ggcgcggtcg
     1081 agatcgtggc cgagaagggc accaacacgc gctggggcgg ggccgagggc aacgccgggc
     1141 cggtggtgcg cgaccgctgc atcgccaacg gcctgatggt ccgcgggatc cgcgacacca
     1201 tcgtgatgtg cccgccgctg atcatcaccc cggcccagat cgacgacatg gtcgcgatca
     1261 tcgccaagtc cctggacgag gccctgccca agctgcaggc actctaagcc caagtccgct
     1321 tgcgcgcgcg cggcctagcc gataggccgc gcgccatgcg ccgcaccctc ctgactgctt
     1381 ccgccctgat cgccgcaccc ggcctggccc tggcccagcc ggcggaggaa ccgggtgacg
     1441 cgccgacggg cgagatcgtg gtgaccggcc aggggctcga tccctcgccg ttgctggcgg
     1501 cctattcggc cgtcgcgatc cgccgcgaca gcgccgctcc gccggcatcg ggccggctgg
     1561 aagacgtgct ggccagcgcc gccgggttcc agcagttccg ccgctcggac agccgctctg
     1621 ccaaccccac ggcccagggc gtcaccctgc gcgggctggg cggcaacgcg accagccgga
     1681 cgctggtggt gcttgatggg gtgccggtga ccgatccgtt ctttggccac gtgccgttta
     1741 ccgcgctgcc gttcgagcgg ctggagcggg ccaccgtgat ccgcggcggc ggctcgggcg
     1801 ccttcggggc cggggccgtg gccggaacgc tcgacctggc cagcgccaac ccggccgatt
     1861 tgccgctgct ggaagcggcg ctgcttggca acgatcgcgg cgagagcgag ctttcggcca
     1921 cgctcgcccc ccggcttggc gcgggctacg ccgtggtcag cgggcgctgg gaccggggcc
     1981 aggggttctg gaccacgccg ctggcgcagc gcgtccccgc cagcgcgcgg gcccggtttg
     2041 acagctggtc cacctcgatc cgcgcggtcg cgcctctggc cgaagggatc gagctccagg
     2101 cccgcgccct ggtgttcgag gaccgccgca ccttgcgctt tgccggggcg gacagctttc
     2161 agggcgggca ggacctgtcc ttgcggctgg tcgggcgcgg ggcctggcag gtcgatgtgc
     2221 tgggctatgt ccagcagcgc aatttctcca acgtggtgat cagcgccacc agctttcgcc
     2281 gcacgctgga ccagcagggc acgccggcca ccgggatcgg cggcaagatc gaactgcgcc
     2341 cgccggtcga cgccgcgcat tcgctgcgct tcgggatcga ttaccgccgg tccagcggcg
     2401 agctgcagga ggttgcctac agcggcgtga ccggggcggt tactgcgcgg cggcgggccg
     2461 gcgggaccaa ccaggacctg ggcttttatc tggagcacga ccgcgatctg ggtccgctgc
     2521 ggctgaccgg ctcggtccgg ctcgatcgct ggtcggtcag cgacgggcgg ttccgggaaa
     2581 gcaacgcggc cggggcggtg gtcagcgatc tggcctatcc ggaccggtcg ggctgggacc
     2641 tttcgctgcg cggcggggtt ctggccgatc tggccccggg cctggtgctg cgcggatcgg
     2701 tctacagcgg cctgcgccag ccaaccctca acgagcttta ccgccccttc gtggtcttcc
     2761 ccgtcaccac ccgcgccaac gccgcgctgg agaacgagga cctgttcggc ttcgaaggcg
     2821 ggctcgattt gcggctgggc ggggtgaccc tctcggccac attgttcgac aaccgcattg
     2881 ccggggcgat cgccaatgtc acgatcgggc cgaacctgcg cgagcggcgc aatgtcgatg
     2941 cggtccacgc ccgcgggctg gaactgaacg ggcgggccga gctgggccgg gtgacctggg
     3001 acggatcgct ctcgctg
//
LOCUS       DASUDV010000341         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1049675,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000341 DASUDV010000000
VERSION     DASUDV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049675"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="VFV30_07480"
     CDS             complement(<1..1964)
                     /locus_tag="VFV30_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847969.1"
                     /translation="MTRRFAPSLIALSALLSGTAAWACADGGCEGGFNLVSKDQSCQG
                     RLMLAPGNDSRINLMLLINDRGGRTTAGLAPPREGVSYSYLESPFLDWPMLREALYPT
                     KEIPWEERQSDYSGSRCQSYASGSGALAQAMAANSALPAAEKTALIAARAQAEAVCKL
                     GTSYERRYKDRADLTPLPVFGQWPAVSSQPGREFLAYMQAADAFYGDRFGEARSGFLA
                     LAGAGDPWVKETAAYMLIRVHFAAAQAPAFGDYGSYDGAEKVDRGSVKQGLEALAAYL
                     RAWPSGRYAGSAKGLLRRGLWLAGDYDALGSGYAGMLERADPRKADAAALVEEVDTKL
                     LFNPDAKGAGAKGPMLLAAKTLAALRDSNYEDNAPAKPGITAAEIDALQPQFAAQPEL
                     FSYLQATHAFYYGRDYRRVLTLIPDAARKPSHSNLSFSRQVLRGMALAALKDRNEAGF
                     WQELIPGAQGRWQGALAQLGLAMAWERGGKVEAVFAAGSPVADPSIRRQLITWSAGPV
                     LLRSIVRTPANPAEERDHALNTLLSNQLVLGQYAAFGTDVKLPLADPKSAASMFTRGK
                     VSDGYPCAPLAVTVAALARNPQDIAGRLCLGDFFRINGLDYLRGEYDPAPRPDELGGA
                     AKGFAGKRNFRSEFYTSIIADPRAGANDKA"
BASE COUNT          337 a          757 c          779 g          329 t
ORIGIN      
        1 taggccttgt cgttggcccc ggcgcgagga tcggcaatga tcgaagtgta gaactcgctg
       61 cggaaattgc gcttgccggc aaagcccttg gccgccccgc ccagctcgtc gggacgcggg
      121 gcgggatcgt attcgccgcg cagataatcg agcccgttga tccggaagaa atcgcccagg
      181 cacagccgcc cggcaatgtc ctgcgggttg cgggccagcg ccgcgacggt caccgccagc
      241 ggggcgcagg ggtagccgtc cgaaaccttg ccgcgggtga acatgctggc cgcgctcttg
      301 ggatcggcca gcggcagttt cacatcggtg ccgaaggcgg cgtactggcc cagcaccagc
      361 tggttggaca gcagggtgtt gagcgcgtga tcgcgctcct ccgccgggtt ggccggggtg
      421 cggacgatcg agcggagcag caccgggccg gctgaccagg tgatcagctg gcggcggatc
      481 gacgggtcgg ccaccggcga tccggcggcg aagaccgcct ccaccttgcc gccgcgctcc
      541 cacgccatgg ccaaccccag ctgcgccagc gcgccctgcc agcggccctg cgcgccgggg
      601 atcagctctt gccagaaccc ggcctcgttg cggtccttca gcgcggccag cgccatgccg
      661 cgcagcacct ggcggctgaa ggagaggttg ctgtgcgaag gcttgcgcgc ggcatcgggg
      721 atcagggtca gcacccggcg gtaatcgcgc ccgtaataga acgcgtgggt cgcctggaga
      781 taggagaaca gctccggctg ggcggcgaac tgcggttgca gcgcgtcgat ctcggccgcg
      841 gtgatgcccg gcttggctgg ggcgttgtct tcgtaattgc tgtcgcgcag ggcggccagg
      901 gtcttggccg ccagcagcat cggccccttg gccccggcgc ccttggcatc ggggttgaac
      961 agcagcttgg tatcgacctc ttcgacaagg gcggcggcat cggccttgcg cggatcggcg
     1021 cgctccagca tcccggcata gccgctgccc aacgcgtcat aatcccccgc cagccacagg
     1081 ccgcggcgca gcaggccctt ggccgatccg gcatagcggc cgctgggcca ggcgcgcaga
     1141 taggcggcga gcgcctccag cccctgcttc accgagcccc gatcaacctt ttccgccccg
     1201 tcataggacc catagtcgcc aaaggcgggc gcctgggccg cggcgaagtg aacgcggatc
     1261 agcatatagg cggcggtttc cttgacccac ggatcgcccg ccccggccag cgccagaaag
     1321 ccgctgcgcg cttcgccgaa ccggtcaccg taaaaggcat cggccgcctg catataggcg
     1381 aggaactcgc gccccggctg gctcgatacg gcgggccact ggccgaacac cggcagcggg
     1441 gtcaggtctg ccctgtcctt gtagcgccgc tcgtaggagg tgccgagctt gcacaccgcc
     1501 tcggcctggg cgcgggcggc gatcagagcg gtcttttccg ccgcgggcag cgccgaattg
     1561 gcggccatgg cctgggccaa tgcgccgctg cccgaggcgt agctttggca gcggctgccg
     1621 gaatagtcgg actgccgctc ctcccagggg atctctttgg tcgggtagag cgcctcgcgc
     1681 agcatcggcc aatcgaggaa cggcgattcg aggtaggaat agctcacccc ttcgcgcggc
     1741 ggggcaaggc cggccgtggt ccgcccgccg cgatcgttga tcagcagcat caggttgatc
     1801 cggctgtcgt tgccgggggc cagcatcagc cggccctggc agctctggtc cttggagacg
     1861 aggttgaacc cgccctcgca gccgccatcg gcgcaggccc aagcggcggt gccggacagc
     1921 agggcggaga gggcaatcag cgaaggcgcg aaacggcggg tcatggcggg tcctcctcgg
     1981 cggcgagaat cccctgcttg ccgcatcggc gcaagcaaaa agggggccgc aaacgcagcc
     2041 ccctttcgca ttttttcccg taccgggctt ggacttaatc gtcgcccttg aggaaggccg
     2101 ggacgtggtc cggagccgga ccgtcatcac ggtcgcggcg cgggccgcga tcgccaccct
     2161 cacggcgcgg accgcgatca cggtcacggc cgccacggtc gc
//
LOCUS       DASUDV010000342         6670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_894754,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000342 DASUDV010000000
VERSION     DASUDV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894754"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFV30_07485"
     CDS             complement(<1..229)
                     /locus_tag="VFV30_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX4847970.1"
                     /translation="MSLRPDLAIIAANVAPGARVLDIGCGDGALLAALRAEKQVDARG
                     MELDPHGVADCVAKGLSVMQGDADTDLADYPD"
     gene            complement(226..1347)
                     /locus_tag="VFV30_07490"
     CDS             complement(226..1347)
                     /locus_tag="VFV30_07490"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX4847971.1"
                     /translation="MAAADLNLVNDTLVLPAPLPLDGGQSLERVEVAYETYGTLNAEK
                     SNAVLICHALTGDQHVASPHPKTGKPGWWVRTVGPGKVIDTSRYFVICANVLGSCMGT
                     TGPASPAADGKPWGMRFPVITIRDMVRTQVALLDALGIDQLHAVIGGSMGGMQALSFA
                     ANWPQRTRAVLVIASTARHSAQNIAFHEVGRQAIMADPKWKGGDYYGARGSGSKGPEN
                     GLAVARMAAHITYLSEAGLTEKFGRRLQDRTEKTFGFDADFQVESYLRHQGLSFTDRF
                     DANSYLYITRAMDYFDLAEEHGGRLADAFAGVKARFCLVSFDTDWLYPTAESRVIAHA
                     LNAAGAPVSFVELSAPFGHDSFLLEVPAQDRVIQGFLNQ"
     gene            1414..2511
                     /locus_tag="VFV30_07495"
     CDS             1414..2511
                     /locus_tag="VFV30_07495"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX4847972.1"
                     /translation="MSGQPIPKPWIAGIHAYVPGKSAGADGRPLVKLSANENPLGCSP
                     DALAARETAVHPSLYPDGDSTALREGVGQLHGIDPARIIMGAGSGELLYLAASAYAGP
                     GDEVLFSQYSFSLYDIVARRSGADPVEAPARDYGTDVDAMLAAVTDRTRVVIVANPNN
                     PTGTYLPRAEIARLHAGLPAEVLLVLDQAYAEYVDPADDDGSLDLAASQPNVLVTRTF
                     SKAYGLAGDRVGWGTAAAGVIEVLNRLRGAFNVCNTAQAGALAALADQAFVENSRLHN
                     ARVRADFVAAIGQLGNHGIRAVPSEANFVMVLLEGRLSGEAAYNGLAEAGYITRWLPG
                     QGLGHGLRITIGTAAQMAEIAAILRSLAEAA"
     gene            2511..3422
                     /locus_tag="VFV30_07500"
     CDS             2511..3422
                     /locus_tag="VFV30_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEX4847973.1"
                     /translation="MAFERIAIIGLGLLGGSIGLALKEHVPGAVTTGYDTDPATRARA
                     AERGLVGTVCASAAEAVAGAGLVILCVPVGAMNTAAAEIAGHLAPDAVVSDVGSSKQS
                     VAEALAAALPGVTVIPAHPVAGTERSGPDAGFASLFQNRWCIITPPADADPVKVAALS
                     ALWEALGAKVEIMDARHHDMVLAVTSHLPHLIAYTIVGTASDLEGVTESEVIKYSAGG
                     FRDFTRIAASDPTMWRDVFLSNKDAVLAMLQRFTEDLTALQRAIRVGDGDQLFDHFTR
                     TRAIRRSIIEEGQDDARPDFGRSDHRG"
     gene            complement(3561..4817)
                     /locus_tag="VFV30_07505"
     CDS             complement(3561..4817)
                     /locus_tag="VFV30_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX4847974.1"
                     /translation="MSGEPAKRSPFEVLGPGLVTGAADDDPSGIGTYSQVGAQFGYGL
                     AWTMFFGFPLLASIQAICARIGATTGKGIAQNLRQHYSPWLLRAVVLLLLFANVINLG
                     ADLGAMGAVLALVLPGPVLAYTVAFGVVSVLLEVFLSYSVYARILKWTTLSLFAYVGV
                     VFVADVPLWEAARGTLIPSFTFDKAHAMALVAIFGTTISPYLFFWQAGQEVEEQHRRH
                     IKPLHVSPRRAGAELRRIRTDTLVGMGFSHLVALFIVIATAATLHANGVRDIESAAQA
                     AEALRPIAGDFAFALFATGIIGTGLLAVPILAGSAAYAVSETFGWTEGLDRKPREAKA
                     FYGVIALATAGGVALNFIGIDPMKALYWAAVVNGLLAPPLMVVTMLIARNRKIMGKLA
                     ISSGLELGGWAATAVMWIVAGVFLLS"
     gene            complement(4814..5512)
                     /locus_tag="VFV30_07510"
     CDS             complement(4814..5512)
                     /locus_tag="VFV30_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4847975.1"
                     /translation="MGTVLAMVRSIAFYAIFYSGSVLIVLASLAALALGRETFQWTVD
                     RWARWHRLCMRVLLGQTVRFEGTLPDGPIFVAIKHESYFEAIDLARLLRRPGVFAKAE
                     LMRIPLWGRVGDRYGLVEVQRDQGAKALRHMMAAARKLSGEEGRPLVIFPEGTRVPQG
                     ARPPLQSGFAGIYKLLGLPVVAIAVDSGRLYHRWWKLPGVITYRVVDVVPPGLPREEA
                     EARVHAAINALNTP"
     gene            complement(5514..6044)
                     /locus_tag="VFV30_07515"
     CDS             complement(5514..6044)
                     /locus_tag="VFV30_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX4847976.1"
                     /translation="MLRRVLSLILIAWALGFIWFAVALPQPAGDERTDGIVVLTGGKG
                     RIDRGLEALRGGWAPKLLISGVDRSVKPHEFAIEYRIGSRQMACCVTLGFAATDTATN
                     ARETAEWIKRQNMTSIRLVTSDWHMRRAVSELKQTIPAGVTIVPDAVATQPSLRMLFL
                     EYHKLLARPVLRLVGA"
     gene            complement(6049..>6670)
                     /locus_tag="VFV30_07520"
     CDS             complement(6049..>6670)
                     /locus_tag="VFV30_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEX4847977.1"
                     /translation="RVVSQAEVEKLIEPWLGARAGDGQIPVPALVDVRLREAVDGEAL
                     GALRRLVRQVAPAARVDAQSTWLKPVFGAIDSLRWLSLALIGLLSFALAAAVLLAVRT
                     ALGTNRDTIEIVHLLGGTDAQIARVFQRTIGLDAASGGAVGLILGVVTVVFLGRRFAG
                     LGAGMVNSGALGWIDWLLLALVPIAGVILAVVTARFTVLRALRRML"
BASE COUNT         1101 a         2351 c         2245 g          973 t
ORIGIN      
        1 catcgggata gtcggccaga tcggtatcgg cgtcgccctg catcaccgac aggcccttgg
       61 cgacgcaatc ggccaccccg tgcggatcga gttccatgcc gcgggcatcg acctgctttt
      121 cggcccgcag cgccgccagc agcgcgccat cgccgcagcc gatatccagc acccgcgcac
      181 ccggcgcgac attggcggcg atgatcgcaa gatcgggacg caggctcatt ggttgaggaa
      241 cccctgaatg acgcggtcct gggccggcac ctccagcagg aagctgtcat gcccgaacgg
      301 ggccgacagc tcgacaaagc tgaccggcgc acccgccgcg ttgagcgcgt gggcaatcac
      361 ccggctttcg gcggtgggat agagccagtc ggtatcgaag ctgaccaggc agaaccgcgc
      421 cttcaccccg gcaaaggcat cggccagccg cccgccgtgt tcctcggcca ggtcgaagta
      481 atccatcgcc cgggtgatat agagatagga attggcatcg aaccggtcgg taaagctcag
      541 cccctggtgg cgcagatagc tttcgacctg gaaatcggcg tcaaagccaa aggtcttttc
      601 ggtccggtcc tgcaggcggc ggccgaactt ctcggtcagg ccggcttcgc tgaggtaggt
      661 gatgtgcgcc gccatgcggg cgacggcgag gccgttttcc gggcccttgc tgccgctgcc
      721 ccgggctccg taatagtccc cgcccttcca cttgggatcg gccatgatcg cctggcggcc
      781 cacctcgtga aaggcgatgt tctgcgccga atggcgggcg gtgctggcga tcaccagcac
      841 ggcgcgggtc cgctggggcc agttggcggc aaaggacagc gcctgcatcc cgcccatcga
      901 tccgccgatc accgcgtgca gctgatcgat ccccagcgca tcgagcaacg ccacctgggt
      961 gcggaccatg tcgcggatgg tgatgaccgg aaagcgcatg ccccagggct tgccatcggc
     1021 ggccgggctg gcggggccgg tggtgcccat gcacgatccc agcacgttgg cgcagatcac
     1081 gaaatagcgg ctcgtatcga tcaccttgcc cggtccgacc gtgcggaccc accagccggg
     1141 cttgccggtt ttcgggtgcg ggctggccac gtgctgatcg ccggtcagcg cgtggcagat
     1201 caggaccgcg ttcgatttct ccgcgttgag cgtgccgtag gtttcatagg ccacttcgac
     1261 gcgctcaagg ctttgcccgc catcaagcgg gagcggcgcg ggaaggacca gggtgtcgtt
     1321 gaccaggttg agatcggcgg ctgccatgcc tgccgcttgg gggaggagaa gcatgctgtc
     1381 aattcccggc aagcctgcta agggcggcgc gctatgagcg gacagcctat ccccaaaccc
     1441 tggatcgcgg gtattcacgc ctatgtcccc ggcaagagcg ccggcgcgga cgggcggccg
     1501 ctggtcaagc tatcggccaa cgagaacccg ctgggctgca gcccggacgc gctggccgcg
     1561 cgcgagacgg cggttcaccc ctcgctctat cccgatgggg acagcacggc cctgcgcgaa
     1621 ggcgtcggcc agcttcacgg gatcgatccg gcgcggatca tcatgggcgc cggttcgggc
     1681 gaactgctct acctcgcagc cagcgcctat gccgggccgg gcgacgaggt gctgttctcg
     1741 cagtactcgt tctcgctcta cgatattgtc gcgcggcgca gcggggccga cccggtcgaa
     1801 gcgccggcgc gtgactatgg caccgatgtc gatgccatgc tggccgccgt gaccgaccgg
     1861 acccgggtgg tgatcgtggc caatcccaac aacccgaccg gcacctacct gccgcgcgcc
     1921 gagatcgccc ggctgcacgc cggcctgccg gccgaagtgc tgctggtgct cgatcaggcc
     1981 tatgccgaat atgtcgatcc ggccgacgat gacggttcgc tggatctggc ggcaagccag
     2041 cccaacgtgc tggtgacccg cactttctcc aaggcctatg gcctggccgg agaccgggtt
     2101 ggctggggca ccgcggcggc cggggtgatc gaagtgctga accgcctgcg cggcgccttc
     2161 aacgtctgca acaccgccca ggccggagcg ctggcggcgc tggcggacca ggcatttgtc
     2221 gaaaactcgc ggctccacaa cgcccgcgtc cgggccgact ttgtcgccgc gatcgggcag
     2281 ctgggcaatc acggcatccg cgccgtgccg agcgaggcca acttcgtgat ggtgctgttg
     2341 gaagggcggc tgagcggcga ggcggcctat aacggcttgg ccgaggccgg ctatatcacc
     2401 cgctggctgc ccggccaggg gctgggccac ggcctgcgga tcacgatcgg caccgcggcg
     2461 cagatggcag agattgccgc gatcctgcgc agcctggcgg aggcggcgta atggcgttcg
     2521 aacggatcgc gatcattggc ctcggcctgc tgggcggctc gatcgggctg gcgctgaagg
     2581 agcatgtgcc cggcgcggtg accaccggct atgacaccga tcccgccacc cgcgcccgcg
     2641 ccgccgagcg cggcctcgtc ggcacggtct gcgccagcgc ggccgaggcc gtggccggtg
     2701 ccgggctggt gatcctgtgc gtgccggttg gcgcgatgaa caccgccgcc gccgaaatcg
     2761 ccgggcatct ggcccccgac gcggtggtga gcgatgtcgg ctcgtccaag cagtcggtcg
     2821 ccgaagccct ggccgcggcg ctgccggggg tgaccgtgat ccccgcccac ccggttgccg
     2881 ggaccgaacg gtccggtccg gacgcgggct ttgccagcct gttccagaac cgctggtgca
     2941 tcatcacccc gccggccgat gccgatccgg tgaaggtcgc cgcactctcg gcgctgtggg
     3001 aggcgctggg cgccaaggtc gagatcatgg acgcgcggca ccacgatatg gtgctggccg
     3061 tcaccagcca cctgccgcac ctgatcgcct ataccatcgt tggcaccgcc agcgatctgg
     3121 agggcgtgac cgaaagcgaa gtgatcaagt attcggccgg cggcttccgc gatttcaccc
     3181 gcattgccgc ttcggatccg accatgtggc gcgacgtgtt cctgtccaac aaggacgcgg
     3241 tgctggcgat gctgcagcgc ttcaccgaag acctgaccgc gctgcaacgc gcgatccggg
     3301 tgggcgacgg cgaccagctg ttcgaccatt tcacccgcac ccgcgcgatc cgccgctcga
     3361 tcatcgagga aggccaggac gacgcccggc cggacttcgg acgcagcgac caccgggggt
     3421 gacagtcttg tccgcctcgc ggcggacgca tcaccggccc gctcccccgg cccaaccgcc
     3481 cgttaaaggt accctgtggg cggttgggcc gaggaagcgg gccggtgatg caacagcagg
     3541 ccggatggcc tgctcgaccg tcagctcaac aggaataccc cggcgacgat ccacatcacg
     3601 gctgttgccg cccatccgcc aagctcaagt ccgcttgaga ttgccaattt gcccatgatt
     3661 ttgcggttgc gggcgatcag catggttacc accatcagcg gcggggccag cagtccgttg
     3721 accaccgcgg cccagtacag cgccttcatc ggatcgatcc cgatgaagtt cagcgccacc
     3781 ccgccggccg tggcgagcgc gatcacgccg taaaaagcct tcgcttcgcg cggcttgcgg
     3841 tccagacctt cggtccagcc gaaggtttcg ctgaccgcat aggcggcgct gcccgccagg
     3901 atcggcacgg ccagcaggcc cgtcccgatg atcccggtgg cgaacagcgc gaaggcgaaa
     3961 tcgccggcga tcgggcgcag cgcctcggcc gcctgggccg cgctttcgat atcgcgcacc
     4021 ccgttggcgt gaagcgtcgc cgccgtagcg atgacgatga acagcgcgac caggtgggaa
     4081 aagcccatgc cgaccagcgt atcggtgcgg atccggcgga gctcggcccc ggcgcggcgc
     4141 ggactgacgt ggagcggctt gatgtggcgg cggtgctgtt cttccacttc ctgcccggcc
     4201 tgccagaaga acaggtaggg gctgatcgtg gtgccgaaga tcgcaaccag cgccatcgcg
     4261 tgcgccttgt caaaggtgaa gctggggatc agcgtcccgc gcgcggcctc ccacagcggc
     4321 acgtcggcga cgaagaccac cccgacatag gcaaacagcg acagcgtggt ccacttcagg
     4381 atccgggcat agaccgaata gctgaggaac acctcgagca ggacgctgac cacgccgaag
     4441 gccacggtat aggccagcac cggccccggc aggaccagcg ccagcaccgc gcccatcgcc
     4501 cccaggtccg cccccaggtt gatcacgttg gcaaacagca gcagcaggac caccgcgcgc
     4561 agcagccagg gcgaatagtg ctggcgcagg ttctgggcga tgcccttgcc ggtggttgcc
     4621 ccgatccgcg cgcagatcgc ctggatcgag gccagcagcg gaaagccgaa gaacatcgtc
     4681 caggccaggc catacccgaa ctgggcgccg acctggctgt aggtaccgat ccccgaagga
     4741 tcgtcgtcgg ccgcgcccgt caccagcccc ggaccaagca cctcgaaggg tgagcgcttg
     4801 gccggttccc cgctcacggg gtgttcagcg cgttgatcgc ggcgtggacc cgggcttcgg
     4861 cctcctcgcg cgggaggccc ggcgggacca catcgaccac gcggtaggtg atcaccccgg
     4921 gcaatttcca ccagcggtga tagagccgcc cagaatcgac cgcgatggcc accaccggca
     4981 ggcccagcag cttgtagatc ccggcaaagc ccgattgcag cggcggacgg gcgccctgcg
     5041 gcacgcgggt gccttcggga aagatcacca gcggccggcc ttcctcgccg ctcagcttgc
     5101 gggccgcggc catcatgtgg cgcagcgcct tggcgccctg gtcgcgctgc acctcgacca
     5161 ggccatagcg gtcaccgacc cgcccccaga gcgggatccg catcagctcg gccttggcga
     5221 agacgcccgg ccgccgcagc aggcgggcca ggtcgatcgc ctcgaaatag ctttcgtgct
     5281 tgatcgcgac gaagatcggg ccgtccggca gggtgccttc aaaccgcacg gtctggccca
     5341 gcaggacgcg catgcacagc cggtgccagc gggcccagcg gtccacggtc cactgaaagg
     5401 tctcgcggcc cagcgccagc gcggccaggc tggccagcac gatcagcacg ctgccgctgt
     5461 agaagatcgc atagaacgcg atcgagcgga ccatggccag cacggtcccc atatcaggcc
     5521 cccaccaggc gcaggaccgg acgcgccagc agcttgtggt attccaggaa aagcatccgc
     5581 aggctgggct gggtcgccac cgcatcgggc acgatcgtca ccccggcggg gatcgtctgc
     5641 ttcagctcgg acaccgcgcg gcgcatgtgc cagtcgctgg tcaccaggcg gatcgaggtc
     5701 atgttctggc gcttgatcca ttccgccgtc tcgcgggcgt tggtggccgt atcggttgcg
     5761 gcaaagccca gcgtgacgca gcaggccatc tggcggctgc cgatgcggta ttcgatcgcg
     5821 aattcgtgcg gcttgacgct gcggtccacg cccgagatca gcagcttggg tgcccagccg
     5881 ccgcgcagcg cttccagccc gcggtcgatc cggcccttgc cgccggtcag cacgacgatc
     5941 ccgtcggtcc gctcgtcccc ggccggctgc ggcagggcca cggcaaacca gatgaagccc
     6001 agggcccagg cgatcaggat cagggaaagg acgcgccgca gcatggccct agagcatccg
     6061 ccgcagcgcg cgcagaacgg taaagcgcgc ggtcaccacc gccaggatca ccccggcaat
     6121 cggcaccagg gccagcagca gccagtcgat ccagcccagc gcgcccgaat tgaccatccc
     6181 ggcccccagc ccggcaaagc ggcggcccag aaagaccacc gtcaccaccc ccaggatcag
     6241 gccgaccgcg ccgccgctgg cggcatcgag gccgatggtg cgctggaaca cccgggcgat
     6301 ctgggcgtcg gttccgccca gcaggtggac gatctcgatc gtgtcgcggt tggtgcccag
     6361 cgcggtgcgc accgccagca gcaccgccgc cgccagcgcg aagctgagca ggccgatcag
     6421 cgccagcgag agccagcgca gcgaatcgat cgccccgaac accggcttca gccaggtcga
     6481 ttgcgcgtcg acccgcgcgg ccggggccac ttgccgcacc agccgccgca gcgcgcccag
     6541 cgcttccccg tcgaccgctt cgcgcagccg cacgtcgacc agcgcgggca ccggaatctg
     6601 cccgtccccg gcccgcgcgc ccagccacgg ttcgatcagc ttttcgactt cggcctgcga
     6661 caccacgcgg
//
LOCUS       DASUDV010000343        11576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1159972,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000343 DASUDV010000000
VERSION     DASUDV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159972"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFV30_07525"
     CDS             <1..325
                     /locus_tag="VFV30_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX4847978.1"
                     /translation="RIIRQRKLRARFFDGDLFADPDWDMLLDLTAARAEHTRVSVTSL
                     CIASGVPPTTALRWIGQVTEAGLLERVEDETDRRRAFIALTDKAADAMARYFAELGSH
                     AARLV"
     gene            390..465
                     /locus_tag="VFV30_07530"
     tRNA            390..465
                     /locus_tag="VFV30_07530"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:423..425,aa:Val,seq:tac)
     gene            574..2934
                     /locus_tag="VFV30_07535"
     CDS             574..2934
                     /locus_tag="VFV30_07535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX4847979.1"
                     /translation="MDELLGDFIAESREMLEALESEIVAWEADPADRTRLDTIFRFFH
                     TVKGNCGFFDFPRLETLSHAAEDALSEVRSGQREPTAAFVDAVLAVIDRIAGMIDAID
                     AGKDISGGDDTALIESLAHAGSAAEAGEDEAGLTQPGLAPRSAGALRSVRLPINLLDR
                     VMSGVSDMVLARNELARRIADLGEGSGLEAPFNRLSNILTDVRDAITRTRMQRIETLF
                     AGFPRLVRDLSAELGKQVMVEIQGGDVELDREMIEMIRDPLVHILRNAIDHGIETPAQ
                     RRAAGKREIGLLRMSARQTGNQIRIAISDDGRGLDTERLIAKALSAGVLTAEQAERIT
                     PEEARMLVFEPGLSTADAVSAVSGRGVGLDVVRANIERIGGTVAVHSVPGEDCRFLLS
                     LPLTLSIVPSLTVSAGGQRFAIPRSYVDEIFLFDEGQGAFIRAGGRQFLEIRDSKIAC
                     AELAEVLDLPAPAERKARWVVLVRMMAGDVFGLIVDRVLDHHDLVAKPIAPVIMGCGL
                     YVGSTQLDDGSPILMLDVVAIGQAAGLLGELEARGYRPVGPERRDEESPGLPVLLFVG
                     LDGRRRAVAMGAVKRYEDVPVAAINASTGAPRVVIGEVILPLAGLSAALPAEGTVEVL
                     RLTDGEHELVYAVAEVLDTTTIGRDLAPVDNCDEALGLALIGGEAVEVIDPLALFERH
                     GLAEAAPRPPVCRIPSGDAWMQDFLRPLIEQAGYVVVDEHAQDPADVSVLLEGSAAAA
                     APLAAAATVVLRSTRTAASGADGTIYRYDRKAVLAALAAAAEGRAA"
     gene            2931..3377
                     /locus_tag="VFV30_07540"
     CDS             2931..3377
                     /locus_tag="VFV30_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4847980.1"
                     /translation="MSELLLVLSIAGERLAFPAESVSSVIEVESLTPVPRVPDFVAGI
                     SALRSRPLTVLDGSAALGLGPRGGADCGRLGKAVVVEQDRHLYAILVDGIEDVVSATS
                     EPAPPPAKLAPCWQRIALGLVETTAGTVLLADPALLIAGPEQSCAA"
     gene            3432..3797
                     /locus_tag="VFV30_07545"
     CDS             3432..3797
                     /locus_tag="VFV30_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4847981.1"
                     /translation="MKTCLIVDDSRVIRKVTRHMLESLGLAVDEASNGQEALERCDAA
                     MPDVILLDWNMPVMSGIEFILALRARPDGAAPKVVFCTTENDIAHIRQAIDAGADEYV
                     MKPFDHETLLIKLQLVGAA"
     gene            3873..4901
                     /gene="cheB"
                     /locus_tag="VFV30_07550"
     CDS             3873..4901
                     /gene="cheB"
                     /locus_tag="VFV30_07550"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEX4847982.1"
                     /translation="MVVDDSAIVRTVFANIIRREPDLELTATAATAEEGLALLSIRPV
                     DIVLLDLEMPGMGGLDALPRMLAAVPGLQILVVSSLTRAGAEPTLKALALGAADTLAK
                     PTSGKFDDEYRLRLVTRLRALGRLTGRQGPIPSQPPRPAVVTRRPGPKRPQVLAIGAS
                     TGGIHALSHFLGALPATIGLPILVTQHLPASFMEAFARQLVTASGREAVVAQDGEALI
                     ADRILIAPGDAHLTVMRQGKRLVVRHDCAPAASGCLPSVDPMFASLAAACDGEVLGIV
                     LSGMGRDGADGAARIVEAGGTILAQDEASSAVWGMPGAVAKAGLAAAILPPVQLALRI
                     GARPALAS"
     gene            4898..5749
                     /locus_tag="VFV30_07555"
     CDS             4898..5749
                     /locus_tag="VFV30_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX4847983.1"
                     /translation="MNSDDFTARLVGELLNARTGQQLTRDRHWRIDTVLSGLMRSHGI
                     ASAKQLAQRLLLPNSQDLAAAVVEALLNNETYFFRDRAMFDQLAVKILPDLAERRSAS
                     RRLSIWSAGCSTGQEALSLAMIFAEQRARWAGWRIEIVGTDISGKAIAAARKASYSAF
                     QVQRGLGVKQMLDHFSETPEGWQANPALLQPVRFAVHNVLEPAPAPGTFDLILCRNVL
                     LYFDSANRSRALDRLADALSPDGFLMLGGGESAAGLSQRLAPCSETSGFLRRADTQPA
                     RRAAGGR"
     gene            5983..7401
                     /locus_tag="VFV30_07560"
     CDS             5983..7401
                     /locus_tag="VFV30_07560"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX4847984.1"
                     /translation="MALIGAGALVYAVAVAFLIRFSLSRLGRLESYGLTDSLSGVRNR
                     RALHLDIKRLSEEGEERAIALVDLDGFKAVNDQYGHAVGDQVIKRCAVVFTELCGSVG
                     RFYRLGGDEFAIVVTGPIAGNVLEGICHSLLRRLAKPIDMADRSVSLGASIGMARSTG
                     KDGLTSSELLRRADMAMDASKQGGKMRTTWYSPEFDRKREAAQQIEQELREALAGDQF
                     RLQYQPLVDAQTGAIVAVEALIRWERPDGQRIGPNVFIPIAEETGLIDPIGLWVLRKG
                     CTDGLAWDGIKLSINLSSAQLRNPEFPIHLGQILEETGFPPERLELEVTETYLVGDHA
                     SISRSLDLIRGFGVGVVLDDFGTGYASIGFLRKYRFEKLKIDRTLIVDAATDEGSRAM
                     MVSSIAVARAMDMDVTAEGVETEAQADLVRAAGCDQIQGWLYYKAIDAELIPEQLAAR
                     VKRPRKAAARSKTPNGPKAAHG"
     gene            7427..9112
                     /locus_tag="VFV30_07565"
     CDS             7427..9112
                     /locus_tag="VFV30_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4847985.1"
                     /translation="MRGSIAAQLRRIFGISTSLTLVVCLLALASAVWGIRQADWIARQ
                     DQASRMVTAIGDQAAQVMEVEHAGSAAAARTALERVQAAMPAVQDQARALEALAADGS
                     VVPIEQAKALRAETDRLAETLGAAETGQDGAEDRAEASAIAIAGQTRTIAASIGAAQV
                     ASEARNLPIIRFMVAFFALAGLLALGAYLFAMKYFAREFIAPITWLRDALKKLTTGKL
                     DFEVEGTERTDELGDVARAMDELKRFSVEFATMQTQLAERAREDLRKKEEEEHARIAE
                     HNQHAELLRQLADRFEATIGEVVGGVASASTQLQVTATSMASVADQSSHQAADVTRTL
                     GDASAGVTAAAAACDEFALSIGEISRQAATSAELARQATVTASGADQTISALTQSAAQ
                     VGEIVELISSIAQRTNLLALNASIEAARGGEAGRGFAVVASEVKELATQTGKATEEVA
                     QQIRQIQDTTTASVEALRSIVRQIQQLEATSVSIASAVDQQSIAGQDLARSIDVAARN
                     TEEVSSNIVQVRETALATGAAASQVLSSSKELEAQAAALRTKVGDFLGHVRAA"
     gene            9191..10594
                     /locus_tag="VFV30_07570"
     CDS             9191..10594
                     /locus_tag="VFV30_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4847986.1"
                     /translation="MKLEKIENGEPLLDAAVATQCGDLAVGCSDAAGSIERATEHMQV
                     QVEELRALDEIAGSLEADQRHIADSTDEAKLLSAQACERLDNGAERINLAVGEFRSVI
                     DLVSRLGVHVTNFASVMEQVQQVSRSIETIAKTTNMLALNASIEAARAGEAGKTFAVV
                     ASEVKTLAQSSSSAAEEIRQAVSKLVGEAGGLVSEIQRGVDQSVKAEERLETVTIALQ
                     DATQLVARLDEQSDQIARSSAMVHAKGSQVRETVSRIVESVHENAAMLDATRASVVEM
                     EGTSCELFNSVISAGISPRDTAVIDVALGFRDQLVELTQAALDAGDLTMAQLFDSDYR
                     PVPNTNPQLFRTSLSDWADANWRPLFDRARISHPDVRMCSAADMNGFLPTHVSEHSHT
                     PTGDLAYDTKHCRNGRILLDPIDRQAKASTAPFFMAVYRQEGDGRNYLVVRNVYCPVF
                     INGRRWGDFEVAYQIRS"
     gene            complement(10691..11038)
                     /locus_tag="VFV30_07575"
     CDS             complement(10691..11038)
                     /locus_tag="VFV30_07575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4847987.1"
                     /translation="MLDRSRKGSEMPDFTLTDPAGKQVQLASFKGKPVLINLWATWCA
                     PCVAELPALDKLAAQTEGKLTVLAINQDSGQPEKAGEFLKQRGVTRLHPWLDPKNDLA
                     FQFGAETLPITIL"
     gene            11199..>11576
                     /locus_tag="VFV30_07580"
     CDS             11199..>11576
                     /locus_tag="VFV30_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX4847988.1"
                     /translation="MWGGRFAEGPAAIMREINASIPFDKALWRQDIAASKAHVDMLAA
                     QGIVSAEDAAQIAAGLNTIAGEYEQQGVPEDWDLEDIHMTTESRLAELIGPAAGRLHT
                     ARSRNDQVATDFKLWVREAMDAAD"
BASE COUNT         1844 a         3917 c         3929 g         1886 t
ORIGIN      
        1 ccggatcatc cggcagcgca agctgcgcgc gcggttcttc gatggcgatc tgttcgcaga
       61 tccggactgg gacatgctgc tcgatctcac ggccgcccgg gccgagcaca cccgcgtttc
      121 ggtcacctcg ctgtgcattg ccagcggcgt gccgcccacc accgcgttgc gctggatcgg
      181 acaggtgacc gaggccggcc tgctggaacg ggtcgaggac gaaaccgacc gccgccgcgc
      241 cttcattgcc ctgaccgaca aggccgccga tgccatggcg cgctatttcg ccgaactggg
      301 cagccacgcc gcacggctgg tctgatcggc gctttgggct ttcccaacgc gccggcttgg
      361 gctaggggca gcgcccttgg ccacggccgg ggcggttagc tcagttggta gagcatctcg
      421 tttacaccga gagggtcagc ggttcgagcc cgttaccgcc caccaggccc ggcccgcccg
      481 gtccggcaca atcccggccg cgccccccgc gctcctaacc aaagtttaac catgttcgcc
      541 ctaggtccgt gcgcaggacc aggggacatt ccgatggatg agttgctggg cgatttcatc
      601 gccgaatcgc gggaaatgct ggaggccctc gaaagcgaga tcgtggcctg ggaagccgat
      661 cccgccgacc ggacccggct cgacacgatt ttccgcttct tccacacggt caagggcaac
      721 tgcgggttct tcgattttcc ccggcttgaa accctgagcc atgccgccga agacgcgctg
      781 agcgaggtcc gcagcggcca gcgcgagccg accgccgcct ttgtcgatgc ggttctggcc
      841 gtgattgacc ggattgccgg gatgatcgac gcgatcgacg ccggcaagga catctccggt
      901 ggagacgaca ccgccctgat cgaatccctg gcccacgccg gctctgctgc cgaagcgggc
      961 gaggatgagg ccggtctgac ccagcccggc ctggccccgc gcagcgccgg agcgctccgc
     1021 tcggtccgct tgccgatcaa cctgctggac cgcgtgatga gcggcgtgtc cgacatggtc
     1081 ctggcccgca acgaactggc ccgccggatc gccgacctgg gcgaaggatc ggggctggaa
     1141 gcgccgttca accggctctc caacattctc accgatgtcc gcgacgcgat tacccgcacc
     1201 cggatgcagc ggatcgaaac cctgttcgcc ggctttccgc ggctggtccg cgatctctcg
     1261 gccgaactcg gcaagcaggt gatggtcgag atccagggcg gcgatgtcga actggaccgc
     1321 gagatgatcg agatgatccg cgatccgctg gtccacatcc tgcgcaacgc gatcgatcac
     1381 gggatcgaaa ccccggccca gcgccgcgcc gccggcaagc gcgagatcgg cctgctgcgg
     1441 atgtcggccc gccagaccgg caaccagatt cgcatcgcga tcagcgacga cggccgcggg
     1501 ctcgataccg agcggctgat cgccaaggcc ctctcggccg gcgtcctgac cgccgagcag
     1561 gccgaacgga ttacccccga agaggcccgg atgctggtct tcgaacccgg cctgtcgacc
     1621 gccgatgcgg tcagcgcggt gtccgggcgc ggggtcggcc tcgatgtcgt gcgcgccaat
     1681 atcgaacgga tcggcggcac cgtcgcggtc cattcggtgc ccggcgagga ttgccgcttc
     1741 ctgctcagcc tgccgctgac cctctcgatc gtgccctcgc tgaccgtcag cgccgggggc
     1801 cagcgctttg cgatcccgcg gtcctatgtc gacgagattt tcctgttcga cgaagggcag
     1861 ggcgccttca tccgcgccgg tggacgccag ttcctcgaaa tccgcgacag caagattgcc
     1921 tgcgccgaac tggccgaagt gctcgatctg cccgccccgg ctgagcgcaa ggcgcgctgg
     1981 gtggtgctgg tccggatgat ggccggcgat gtctttggcc tgattgtcga ccgcgtgctc
     2041 gatcaccacg atctggtggc caagccgatc gcgccggtga tcatgggctg cgggctctat
     2101 gtcggttcga cccagctcga tgatggcagc ccgatcctga tgctcgatgt ggtcgccatc
     2161 ggccaggccg cgggcctgct gggcgagctg gaggcccggg gctatcgccc ggtcggcccg
     2221 gaacgccgcg atgaagagag cccgggcctg ccggtcctgc tgttcgtcgg tctcgacggc
     2281 cgccgccgcg cggtcgcgat gggggcggtg aaacgctatg aggatgtgcc cgtggcggcg
     2341 atcaacgcca gcaccggcgc accgcgcgtg gtgatcggcg aagtgatcct gccgcttgcc
     2401 ggcctgtccg ccgccctgcc ggccgaaggc acggtcgaag tgctgcgcct gacggacggc
     2461 gagcacgaac tggtctatgc cgtggccgaa gtgctcgaca ccaccaccat cgggcgcgat
     2521 ctggccccgg tcgacaattg cgacgaagcc ctgggcctgg ccctgatcgg cggcgaggcg
     2581 gtggaagtga tcgatccgct ggcgctgttc gaacgccacg gcctggccga agccgcgccg
     2641 cggccgccgg tctgccggat cccttcgggc gatgcctgga tgcaggactt cctgcgcccg
     2701 ctgatcgaac aggccggcta tgtcgtggtc gatgaacacg cccaggaccc ggccgatgtc
     2761 tcggtcctgc tggaaggttc ggccgctgcg gctgcaccgc tggccgcggc cgccacggtg
     2821 gtcctgcgca gcacccgcac cgccgccagc ggcgcggatg gcacgatcta ccgttatgac
     2881 cgcaaggccg tgctcgccgc gctggccgct gctgcggaag ggagggcggc atgagcgaac
     2941 tgctgttggt gctgtcgatc gccggggaac ggcttgcctt cccggcggaa tcggtcagct
     3001 cggtgatcga ggtggaaagc ctgaccccgg tgccgcgggt gccggacttt gttgccggca
     3061 tttccgcgct gcgcagccgg ccgctgactg tcctggacgg cagtgccgcc ctgggcctgg
     3121 gaccgcgcgg gggcgcggat tgcgggcggc tgggcaaggc cgtggtggtg gaacaggacc
     3181 gtcacctcta cgcgatcctg gttgacggga tcgaggatgt ggtctcggcc acgtccgaac
     3241 ccgccccgcc gccggccaag ctggcgccgt gctggcagcg gatcgccctg ggcctggttg
     3301 aaaccaccgc cggaacggtc ctgctggccg atcccgccct gctcatcgcc ggtccggagc
     3361 aatcctgcgc ggcttaatcc tattgttaat gcctaagact agtttgcggc ttgggtcgaa
     3421 ggacaagaac catgaaaacg tgcctgatcg tcgatgattc ccgggtgatc cgcaaggtga
     3481 cccggcacat gctggaaagc ctgggcctgg cggtggacga agcgtccaac ggccaggaag
     3541 cgctggaacg ctgcgacgcg gccatgcccg atgtgatcct gctggactgg aacatgccgg
     3601 tgatgagcgg gatcgaattc atcctcgccc tgcgcgcgcg gcccgatggg gcggcgccga
     3661 aagtggtgtt ctgcaccacc gagaacgaca tcgcccatat ccgccaggcg atcgacgccg
     3721 gggcggacga atacgtgatg aagccgttcg atcacgaaac gctgctgatt aagctgcagc
     3781 tggtgggcgc cgcttgagcc ccgccggtgg accgcgcgcc atcccttgaa ctggatcccg
     3841 atcggtccgc ccggcttgcc ccggtgcggg tcatggtggt cgacgattcg gcgatcgtgc
     3901 gcacggtctt tgccaacatc atccggcgcg agcccgacct tgaactgacc gccaccgccg
     3961 cgaccgccga agaagggctg gcgctgctct cgatccgccc ggtcgatatc gtcctgctcg
     4021 atctggaaat gcccggaatg ggcgggctcg atgccttgcc gcggatgctg gcggcggtgc
     4081 cgggcctgca gatcctggtc gtctcctcgc tgacccgggc cggcgccgaa ccgactctga
     4141 aggccctggc gctgggcgcg gccgatacgc tggccaagcc gacatcgggc aagttcgacg
     4201 acgaataccg cctgcgcctg gtcacccggc tgcgggcgct gggccgcctg accggccgcc
     4261 agggaccgat ccccagccag ccgccgcggc ccgccgtggt aacgcgccgt ccggggccca
     4321 agcgccccca ggtcctcgca atcggggcct cgaccggcgg gatccatgcg ctgagccact
     4381 tccttggcgc gcttcccgcc acgatcggtc tgccgatcct ggttacccag catttgccgg
     4441 cctcgttcat ggaggccttt gcccgccagc tcgttacagc ttcgggccgt gaggcagtgg
     4501 tggcgcagga cggcgaggcg ctgatcgccg accgcatcct gatcgcgccc ggcgatgccc
     4561 atctcactgt catgcgccaa ggcaagcggc tggtcgtgcg ccacgattgc gcgccggcgg
     4621 ccagcggctg cctgccttcg gtcgatccga tgttcgccag cctggccgcc gcctgcgacg
     4681 gcgaggtcct gggcattgtc ctgtccggca tgggccgtga cggcgccgac ggggccgccc
     4741 ggattgtcga ggcgggcggc accattctgg cccaggacga ggccagcagc gcggtctggg
     4801 gcatgcccgg ggctgtggcc aaggccggcc tggcagcggc gatcctgccg cccgtacaac
     4861 tggcgctgcg gatcggcgcg cgcccggcgc tcgcctcatg aacagcgacg atttcaccgc
     4921 gcggctggtg ggcgaattgc tcaacgcgcg aaccggacag cagctgaccc gcgaccgtca
     4981 ctggcggatc gatacggtgc tgtcgggcct gatgcgcagc cacggcattg cctcggccaa
     5041 gcagctggcc cagcgcctgc tgctgcccaa ttcgcaggat ctggccgccg ccgtggtcga
     5101 ggcgctgctc aacaacgaga cctatttctt ccgcgaccgg gcgatgttcg atcagctggc
     5161 ggtgaagatc ctgccggacc tggccgagcg ccgctccgcg agccgccggc tctcgatctg
     5221 gtcggccggc tgctcgaccg ggcaggaggc gctctcgctg gcgatgatct ttgccgaaca
     5281 gcgcgcgcgc tgggccggct ggcggatcga gattgtcggc accgacattt ccggcaaggc
     5341 gatcgccgcc gcgcgcaagg ccagctacag cgccttccag gtccagcgcg gccttggcgt
     5401 caagcagatg ctggatcact tctcggaaac cccggagggc tggcaggcca atccggcgct
     5461 gctccagccg gtgcgctttg cggtgcacaa cgtgctcgaa cccgcgccgg caccgggcac
     5521 gttcgacttg atcctgtgcc gcaacgtcct gctgtacttt gacagcgcca accgcagccg
     5581 cgcgctcgac cggctggccg atgccctctc gccggacggt ttcctgatgc tcggcggcgg
     5641 cgaaagcgcg gccggcctgt cgcagcggct ggcgccgtgt tcggaaacca gcggctttct
     5701 gcgccgggcc gacacccaac cggcgcgccg cgctgctggt gggcgataga taagcgactg
     5761 tatagcgaag taaaaatcga gttaattcgc ttttaaccag cctgcgataa cccctgtgtc
     5821 caacggggga aattcatggg ccgtgctgcc ttcaggaact tttgggatac gcgggtcgcg
     5881 ccaaatctgt tggtgctggt cccggtattt gtcctgaccg gcgtatttgc tttaattcaa
     5941 atacttgcgg atgtcttcaa caccggtgga acccattcgc cgatggcgct gatcggtgcg
     6001 ggcgcgctgg tctatgccgt cgcggtcgcc tttctgatcc gcttcagcct gtcgcggctg
     6061 ggccgactcg aatcgtacgg cctgaccgac agcctctctg gcgtgcgcaa ccgccgggct
     6121 ctgcacctcg atatcaagcg gctgagcgaa gagggcgagg agcgggccat cgcgctggtg
     6181 gacctcgacg ggttcaaggc ggtcaacgat cagtacggcc acgcggttgg cgatcaggtg
     6241 atcaagcgct gcgcggtggt ctttaccgaa ctgtgcggct cggtcggccg gttctaccgc
     6301 ctcggcgggg atgagttcgc cattgtcgtg accggcccga ttgccggcaa cgtccttgaa
     6361 gggatctgtc acagcctgct gcgccgcctg gccaagccga tcgacatggc cgaccgctcg
     6421 gtctcgctgg gggcgagcat cggcatggcc cgctcgaccg gcaaggacgg cctgacctcg
     6481 tccgaactgc tgcgccgcgc cgacatggcg atggacgcct ccaagcaggg cggcaagatg
     6541 cgcaccacct ggtacagccc cgagttcgac cgcaagcgcg aagccgcgca gcagatcgag
     6601 caggaactgc gcgaggcgct cgccggggac cagttccgcc tgcagtacca gccgttggtc
     6661 gatgcacaga ccggggcgat tgtcgcggtt gaggcgctga tccggtggga acgccccgat
     6721 ggccagcgga tcggccccaa cgtgttcatt ccgattgccg aggaaaccgg tctgatcgat
     6781 ccgatcgggc tgtgggtcct gcgcaagggc tgcaccgacg ggctggcctg ggacggcatc
     6841 aagctgtcga tcaacctgtc ctcggcgcag ctgcgcaacc cggagtttcc gatccacctc
     6901 ggccagatcc tcgaggaaac cggcttcccg ccggaacggc tcgaactgga agtgaccgag
     6961 acctatctgg tcggcgatca cgcctcgatc agccgcagcc tcgatctgat ccgcgggttt
     7021 ggcgtgggcg tggtgctgga cgatttcggc accggctatg cctcgatcgg gttcctgcgc
     7081 aagtaccgct ttgaaaagct caagatcgac cgcaccctga ttgtcgatgc cgcgaccgac
     7141 gaaggttcgc gggcgatgat ggtgtcttcc atcgccgtgg cccgggccat ggacatggac
     7201 gtgaccgccg agggcgtgga gaccgaggcc caggccgacc tggtccgcgc cgccggctgc
     7261 gaccagatcc agggctggct ctattacaag gcgatcgacg ccgaactgat ccccgaacag
     7321 ctcgccgccc gggtcaagcg cccgcgcaag gcggcggccc gatccaagac cccgaacgga
     7381 cccaaggcag ctcatggttg atcacgcaca cgatgggagt tggttgatgc gcggttccat
     7441 cgcggcgcag ctgcgccgca tcttcggcat ttcgacaagt ctgacgctgg tggtgtgcct
     7501 gctggcgctg gccagcgcgg tctggggcat tcgccaggcg gactggattg cccgccagga
     7561 ccaggccagc cggatggtca cggcgattgg cgatcaggcc gcccaggtca tggaagtgga
     7621 gcacgccggc tctgccgccg ccgcgcgcac ggccctggaa agggtccagg cggccatgcc
     7681 ggcggtgcag gaccaggccc gcgcattgga agctcttgcg gcggacggtt cggttgtgcc
     7741 gatcgagcag gccaaggccc tgcgcgccga gaccgaccgg ctggccgaga cgctgggcgc
     7801 agccgagacc ggacaggacg gggcggaaga ccgcgccgaa gccagcgcca ttgcaattgc
     7861 cggacagacc cgcacgatcg ccgcgtcgat tggcgctgcc caggttgcat ccgaggcgcg
     7921 caacctgccg atcatccgct tcatggtggc gttctttgcg ctggccgggc tgctggcgct
     7981 gggcgcctat ctcttcgcga tgaagtattt cgcgcgcgaa ttcatcgccc cgatcacctg
     8041 gctgcgcgac gcgctcaaga agctgaccac cggcaagctc gatttcgaag tcgaaggcac
     8101 cgagcggacc gacgagctgg gcgatgttgc ccgggcgatg gacgaattga agcgcttctc
     8161 ggtcgaattc gccacgatgc agacccagct ggccgaacgc gcccgcgagg acctgcgcaa
     8221 gaaggaagag gaagagcacg cccggatcgc cgagcacaac cagcacgccg aactgctgcg
     8281 ccagctggcc gaccgctttg aagcgacgat cggcgaagtg gtgggcgggg tcgcttcggc
     8341 ctccacccag ctccaggtta ccgcgacctc gatggcctcg gtggccgacc agtcctcgca
     8401 ccaggccgcc gatgttaccc gcacgctggg cgatgcctcg gccggggtga ctgccgcggc
     8461 cgccgcctgc gacgaatttg ccctctcgat cggcgagatc agccgccagg ccgcgacctc
     8521 ggccgagctg gcccgccagg cgacggtcac cgcctcgggc gccgaccaga ccatttccgc
     8581 gctgacccag tccgccgccc aggtgggtga gatcgtcgag ctgatctcct cgatcgccca
     8641 gcgcaccaac ctgctggcgc tcaacgcctc gatcgaggcg gcccgcgggg gcgaggcggg
     8701 ccgcggcttc gccgtggtcg cgtccgaagt aaaggaactg gccacccaga ccggcaaggc
     8761 caccgaagag gtggcccagc agatccgcca gatccaggac accacgaccg ccagcgttga
     8821 ggcgctgcgc tcgatcgtgc ggcagatcca gcaactggaa gcgacgtctg tgtcgatcgc
     8881 ctcggcggtc gatcagcagt cgatcgccgg ccaggatctg gcccgcagca tcgatgtggc
     8941 cgcgcgcaac accgaagagg tttcgtccaa catcgtccag gtccgtgaaa ccgcgctggc
     9001 gactggtgcg gcggcctcgc aggtcctgtc ctcgtccaag gaactggaag cccaggccgc
     9061 ggcgctgcgc accaaggtcg gcgatttcct cggccatgtc cgggcggcct agggcgcgtc
     9121 acggttgacg ccctgttaac catgtcccgc tagttttccc gattgaaccc tttcagcctg
     9181 caggtttgcg atgaagcttg aaaagatcga aaacggcgaa cccttgctcg atgcggcggt
     9241 tgcgacccag tgcggcgatc tggccgtggg ctgttcggac gcggccggct cgatcgagcg
     9301 ggcgaccgag cacatgcagg tccaggtcga ggaactgcgc gcgctggacg agattgccgg
     9361 ttcgctggaa gccgatcagc gccacatcgc ggattcgacc gacgaggcca agctgctctc
     9421 ggcccaggcc tgcgagcggc tcgacaacgg ggccgagcgg atcaacctgg cggttggcga
     9481 attccgttcg gtgatcgatc tggtttcgcg tcttggcgtt cacgtcacca atttcgcctc
     9541 ggtgatggag caggtccagc aggtttcgcg ctcgatcgaa acgatcgcca agaccaccaa
     9601 catgctggcg ctcaacgcct cgatcgaggc ggcccgcgcg ggcgaggccg gcaagacctt
     9661 tgccgtggtc gcatcggaag tgaagaccct ggcgcagagc tccagctcgg ccgccgagga
     9721 aatccgccag gcggtcagca agctggtggg cgaggccggc gggctggtct cggaaatcca
     9781 gcgcggcgtc gaccagtcgg tcaaggccga ggagcggctg gaaacggtca ccatcgcgct
     9841 gcaggacgcg acccagctgg tcgcccgtct ggacgagcag agcgatcaga tcgcccgcag
     9901 ctcggccatg gtccacgcca agggcagcca ggtgcgcgaa acggtcagcc ggatcgtcga
     9961 atccgtgcac gagaacgccg ccatgctcga tgccacccgc gccagcgtgg tggagatgga
    10021 aggcacctcg tgtgagctgt tcaacagcgt catttccgct ggcatctcgc cgcgcgacac
    10081 cgccgtcatc gatgtggcgc tgggcttccg cgatcagctg gtcgaactga cccaggcggc
    10141 gctggacgcc ggcgacctga ccatggcgca gctgttcgac agcgattacc gcccggttcc
    10201 gaacaccaat ccgcagctgt tccgcacctc gctgtccgac tgggccgacg ccaactggcg
    10261 gccgctgttc gaccgcgccc ggatcagtca tcccgatgtc cggatgtgct cggccgccga
    10321 catgaacggc ttcctgccga cccacgtctc cgagcattcg catacgccga ccggcgatct
    10381 cgcctatgac accaagcatt gccgcaacgg ccggatcctg ctcgatccga tcgaccggca
    10441 ggccaaggcc agcaccgcgc cgttcttcat ggcggtctac cgccaggaag gtgacgggcg
    10501 gaactatctg gtcgtgcgca acgtctattg cccggtgttc atcaacgggc ggcgctgggg
    10561 cgacttcgag gtcgcttacc agatccggtc ctgatccggc ctacttcgct tcggcaatca
    10621 gcttggcggt ctccggcccg gcccattcgt gcgggccgac atagcgccac acctcgcgcc
    10681 cgtccgagcc ctataggatc gtgatcggca gcgtttccgc gccgaactgg aacgccaggt
    10741 cgttcttcgg gtcaagccag ggatgcagcc gggtgacgcc gcgctgcttc aggaattcgc
    10801 cggccttctc gggctggccg gaatcctggt tgatcgccag caccgtcagc ttgccttcgg
    10861 tctgcgcggc cagcttgtcg agcgcgggca gctccgccac gcagggcgcg caccaggtcg
    10921 cccacaggtt gatcagcacc ggcttgccct tgaacgaggc cagctgaacc tgcttgccgg
    10981 cgggatcggt cagggtgaaa tcgggcatct cgctgccctt gcgcgaccgg tcgagcacgc
    11041 cggccgcgga cggggcgccg gccggggacg cggcgggtgc agcggcttgc ggttgcgcgg
    11101 ggtccgcgct ttgcctatcg cacccggcga caagcacggc agagccaagg attgcggcag
    11161 tgagcaagcg ggaagacagc acgggtagct ccaattcgat gtggggcggg cggttcgccg
    11221 aaggaccggc cgcgatcatg cgcgaaatta atgcctcgat ccccttcgac aaggctctct
    11281 ggcggcaaga tatcgccgcg tcaaaggccc atgtggacat gttggcggcc cagggaatcg
    11341 tttcggccga ggatgcggcg cagattgcgg ccgggctgaa cacgatcgcc ggggaatacg
    11401 aacagcaggg cgtgcccgag gactgggacc tcgaggacat ccacatgacc accgaaagcc
    11461 ggctggccga gctgatcgga ccggccgccg gacggctcca caccgcgcgc agccgcaacg
    11521 atcaggtggc gaccgatttc aagctgtggg tgcgcgaggc gatggacgcc gccgat
//
LOCUS       DASUDV010000344         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2065754,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000344 DASUDV010000000
VERSION     DASUDV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065754"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..674
                     /locus_tag="VFV30_07585"
     CDS             153..674
                     /locus_tag="VFV30_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847989.1"
                     /translation="MRVALVFVLAAGTLAASPLAAGAATTASPARTQITKALKQTLKS
                     DFVPHTAAEIDAAITPVDINGDRIPDWQVDFKDLGTAWCGTGGCRYQLWLGRAKGGPV
                     RVFDRRVRAHERKMQNGRIVFEFDFHGTYCGETGAEACPGIYAWSPKRGRMIALPIPG
                     QVSAKASPIDRDQ"
     gene            complement(668..1825)
                     /locus_tag="VFV30_07590"
     CDS             complement(668..1825)
                     /locus_tag="VFV30_07590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4847990.1"
                     /translation="MTESEDIIEIRRAVQALCADFPGEYWREKDRERAYPGEFVDALT
                     RSGFLAALIPEEFGGSGLSLDAAAVIMEEIQAAGCNGASAHAQMYIMNTLLRYGSAEQ
                     KAQYLPGIANGSLRLQAFGVSEPTSGTDTLSLRTVAVRDGDEYVVNGQKIWTSRAEHS
                     DLMLLLARTTPREQVASKTEGLSIFLVDMREVVGRGMTIKPIRTMMNHSTTEVFFDDM
                     RIPASSLIGEEGKGFRYILSGMNAERILIAAECVGDAKWFIAKATAYAKDRSVFARPI
                     GQNQGVQFPIARCYAQMRAAELMVHHAAQVYDAGGNPGEEANMAKLLASEASWAAADM
                     CVQTHGGFGFAEEYDVERKFREARLYTVAPISTNLILSYLAEHVLGLPRSY"
BASE COUNT          348 a          691 c          671 g          371 t
ORIGIN      
        1 cctaggttcg agccctagtg cgtccaccac cccctcccca gacagcccaa aagtgaactg
       61 caaggcgcat ttgcgcgccg tagctgtcgc atcgttgcaa gctgccggga ttggattggc
      121 atagaaaccg gtcacttgat ggaggttgcc cgatgcgtgt tgccctggtt ttcgttcttg
      181 ccgccggcac tctggccgcg tcaccgctgg cggccggcgc agctacgacc gcatcaccgg
      241 cccggaccca gatcaccaag gcgctgaagc agaccctgaa gagtgatttc gttccgcaca
      301 ccgcggcgga aatcgatgcc gcaatcactc cggtggatat caacggcgac cgcatcccgg
      361 actggcaggt cgatttcaag gaccttggca ccgcctggtg cggcaccggc ggctgccgct
      421 atcagctgtg gctgggccgc gccaagggcg gtccggtgcg tgtctttgac cggcgggtcc
      481 gcgcccatga gcgcaagatg cagaacggcc gcatcgtctt cgagttcgat ttccacggca
      541 cctactgcgg cgagacgggc gccgaggcct gcccgggcat ctacgcgtgg agccccaagc
      601 gcggccgcat gatcgcgctg ccgatcccgg gacaggtcag cgccaaggcc agcccgatcg
      661 accgcgatca gtaagagcgc ggcaggccga gcacgtgctc ggccaggtag ctcaggatca
      721 ggttggtgct gatcggggcg acggtataga gccgcgcctc gcggaacttg cgctccacgt
      781 cgtactcctc ggcaaagccg aagccgccgt gggtctgcac gcacatgtcg gccgcggccc
      841 agctggcctc gctggccagc agcttggcca tgttggcctc ttcgcccggg ttgcccccgg
      901 catcatagac ctgcgccgca tggtggacca tcagctcggc cgcgcgcatc tgggcatagc
      961 agcgcgcgat cgggaactgg acgccctggt tctggccgat cggccgggca aagaccgagc
     1021 ggtccttggc gtaggcggtc gccttggcga tgaaccactt ggcatcgccg acgcattccg
     1081 cggcgatcag gatccgctcg gcgttcatgc cggagaggat gtagcggaag cccttccctt
     1141 cctcgccgat caggctggag gccgggatgc gcatgtcgtc gaagaacacc tcggtggtcg
     1201 aatggttcat catcgtgcgg atcggcttga tcgtcatgcc gcggcccacc acctcgcgca
     1261 tgtcgaccag gaagatcgac agcccttcgg tcttgcttgc cacctgttcg cgcggggtcg
     1321 tgcgggccag cagcagcatc aggtcggaat gctcggcgcg gcttgtccag atcttctggc
     1381 cgttgaccac atattcgtcg ccatcgcgga ccgcgaccgt ccgcagcgag agggtgtcgg
     1441 tcccgctggt cggctccgac acgccaaagg cctgcagccg cagcgaaccg ttggcgatcc
     1501 ccggcaggta ctgcgccttc tgctcggccg aaccatagcg cagcagcgtg ttcatgatgt
     1561 acatctgcgc atgggcgctg gccccgttgc agccggccgc ctggatttcc tccatgatca
     1621 ctgccgccgc atcgaggctg aggcccgatc cgccgaactc ttccgggatc agcgcggcga
     1681 gaaagcccga gcgggtcagc gcatcgacaa actcgcccgg ataggcccgc tcgcggtcct
     1741 tctcgcgcca gtattcgccc ggaaagtccg cgcacagcgc ctgcaccgcg cggcggatct
     1801 cgatgatgtc ttcgctttcg gtcatgccct agcctttaat cgatcgatta agcggtgcaa
     1861 gtcccgccgc tccgcgcttg acctgtgctg acatcatggc aagttgccaa tgaaaacggg
     1921 atggagggga ttacgatgcg caagacgtat gcgctggcgg ctgcattgct cgcaacgagc
     1981 gtgctgaccg gcggctgcga cagggtcagc cagactttgg gcggctctga agcagtcgct
     2041 gacacggctt atccggaacg ggtctattgg ggcgacaccc a
//
LOCUS       DASUDV010000345         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1204239,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000345 DASUDV010000000
VERSION     DASUDV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204239"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..105
                     /locus_tag="VFV30_07595"
     tRNA            32..105
                     /locus_tag="VFV30_07595"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Gln,seq:ctg)
     gene            210..1082
                     /locus_tag="VFV30_07600"
     CDS             210..1082
                     /locus_tag="VFV30_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX4847991.1"
                     /translation="MTEEEKEKRLHRRRFYKAEQEAGFVESQDHSTPQTRSPAYKLAF
                     RDTDFLLREELRPVRFQLELLKCEMLLDEANIGSTLVCYGSARIPSPEVAAEFVANAA
                     PENKAVAERLAANAKYYEEARKLAHIASSCGIVEQGMRQFVVCSGGGPSIMEAANRGA
                     IEAGAETIGLNIVLPHEQAPNTYVTPRLAFQFHYFALRKMHFLLRAKAVAVFPGGFGT
                     FDEFFELLTLVQTGKMKPIPILLFGREFWTKVVNFEGLAEEGVISPKDLDLFHWVETA
                     DEAWAKIVEFYGLD"
     gene            1189..3300
                     /locus_tag="VFV30_07605"
     CDS             1189..3300
                     /locus_tag="VFV30_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4847992.1"
                     /translation="MRSIHVLSLLAAGAALVPAALIAQAVVGAPRVVPLTETVHGITL
                     SDEYRWMEDPATRPEWAGWVSAESQRTGAMLGAMPQRAVFADSLARISSSLTRQSGYS
                     RAGPTEIWRKSEPGDPVARLMVRDARGTRVLVDPGKVDGDTLASMGAVTLSPDGRTVA
                     VHLSSGGSEVGSIRFFDVASGKETAQPVTGIWGEFPVTFLPGGMIAYTQMAAEPVGGD
                     PLKGMTAWIRPLAGGTPVKVMGGQTGGAAIADNAFPVVLETPGSPFVGGIAGGARVDY
                     EIFLATRTSLLAGKPEWQRLASYDDKIGGGAVRGTTAYLLSAKTNPARDVLVRPLDAK
                     GRPGAATVLMKGNDRLILTGITAARDGIYISATTDGAGRLFFTPGGKAAVREVKLPFE
                     GSILGPDAQPDGNGVSFGLTGWTNNMTSFTARGGVVRATGIASQSWSGASGIGVQRLE
                     AVSADGTRVPLVVLRSKGTSGPVPTLVEAYGGYGIDTAAPYYDRNGMAWLAKGGAMAF
                     CGVRGGGERGRSWHEGGRGPNKPRGMEDLIACAETLTAKGIAPARGPVATGASMAGTL
                     VPTAVLRKPSAFGGMITQVGVVNPVRIGQAENGANQFAEIGDPSDPKQFADLVAMDAY
                     HMIPKAAGLPPSLMVIGMNDRRVVPWMTAKFVARAQAKWPGTVWLRGDDKAGHGVGTT
                     EEVRRSEWADIYAFAWWTQTK"
     gene            complement(3306..4055)
                     /gene="thiD"
                     /locus_tag="VFV30_07610"
     CDS             complement(3306..4055)
                     /gene="thiD"
                     /locus_tag="VFV30_07610"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX4847993.1"
                     /translation="MAPPRILSIAGSDSSGGAGIQADIKTITMLGGYAMTAITAITAQ
                     NTLGITAVEVLSPELIGAQIDACVSDIGVDAVKIGMLGSPAIAAVVAERLERLGVPVV
                     FDPVMIATSGAALADAETIAAFERLMQLAVLTTPNVPELEALGGHEGMAARGIAYLAK
                     GGDAEGERVEDVLCRPGEEPHVMAATRIHTRHTHGTGCTLSSAIATYLGRGLELPEAV
                     DRARLFVRSALRAAPGFGAGSGPLGHTEARG"
     gene            complement(4055..4279)
                     /locus_tag="VFV30_07615"
     CDS             complement(4055..4279)
                     /locus_tag="VFV30_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX4847994.1"
                     /translation="MLEMRPDCEKCGTDLPADLPGAFICSFECTFCTECADGLDERCP
                     NCGGELMDRPTRAKALHAKHPPSTARKFKG"
BASE COUNT          696 a         1432 c         1528 g          683 t
ORIGIN      
        1 gccgcgcccg cgactagagg cacccgccta ttgcccgatc ctctaatggt aagagagcgg
       61 actctgactc cgtcaatcaa ggttcgaatc cttgtcgggc atccaacttc cccaaccgat
      121 cgccacggtc cggcctcgaa aagagcgccg gattttcgcg ttttagggga tgttttgaca
      181 gcggtgccgg gaaaggcgaa aagaacccaa tgactgaaga agaaaaggaa aagcggctcc
      241 accgtcgccg cttctacaag gccgagcagg aagccggttt tgtcgaatcg caggatcact
      301 ccaccccgca aacccgcagc ccggcctaca agctggcctt ccgcgacacc gatttcctgc
      361 tccgcgagga gctgcgcccg gtccgctttc agctggaact gctgaagtgc gaaatgctgc
      421 tggacgaggc gaacatcggc tcgaccctgg tctgctacgg ctcggcccgg atcccttcgc
      481 ccgaagtggc ggccgaattc gtcgccaatg ccgctccgga gaacaaggcg gtggccgaac
      541 ggctggccgc caacgccaaa tattacgagg aagcgcgcaa gctggcccac atcgccagca
      601 gctgcgggat cgtcgaacag ggcatgcgcc agttcgtggt ctgctcgggc ggcggcccct
      661 cgatcatgga agcggcgaat cgcggcgcga tcgaggcggg ggccgaaacc atcggtctca
      721 acatcgtcct gccgcatgag caggcgccga acacctatgt cacgccgcgc ctggcgttcc
      781 agttccacta cttcgcgctg cgcaagatgc acttcctgct gcgcgccaag gcggtggcgg
      841 tgttcccggg cggttttggc accttcgacg aattcttcga actgctgacc ctggtccaga
      901 ccggcaagat gaagccgatc ccgatcctgc tgttcgggcg cgagttctgg accaaggtgg
      961 tcaatttcga aggcctggcc gaagaagggg tgatcagccc caaggacctc gacctgttcc
     1021 actgggtgga aaccgcggac gaggcctggg ccaagatcgt ggaattctac gggctggact
     1081 gaccgggccg ggttgcactg cgcgccaagc aggctagtct tcgggaccag gccggaaaac
     1141 cgggtcccaa gggttgcgca tgtttgacct gctcggggga ggcctgttgt gcgctcaatc
     1201 cacgttcttt cgctgctcgc ggccggtgcc gcccttgttc ccgccgccct gatcgcccag
     1261 gcggtggtcg gcgccccgcg ggtggtgccg ctgaccgaga cggtccacgg gatcaccctc
     1321 agcgacgaat accgctggat ggaagacccg gcgaccaggc cggaatgggc cggctgggtc
     1381 agcgccgaat cgcagcggac cggtgccatg ctgggcgcca tgccgcagcg cgcggtcttt
     1441 gccgattcgc ttgcccggat ctcgtccagc ctgacccggc agtccggcta cagccgcgcc
     1501 ggaccgaccg agatctggcg caagtcggaa ccgggggatc cggttgcccg gctgatggtg
     1561 cgcgatgcca ggggcacccg cgtgctggtc gatcccggca aggtcgatgg cgacacgctg
     1621 gcatcgatgg gggcggtcac tctttcaccg gacggccgga ccgtggcggt gcacctgtcc
     1681 agcggcggat cggaagtggg cagcatccgg ttcttcgatg tcgccagcgg caaggaaacc
     1741 gcccagccgg tgaccggcat ctggggcgaa tttccggtca cgttcctgcc cggcgggatg
     1801 atcgcctata cccagatggc ggcagagccg gtcggcggcg atccgctgaa gggcatgacg
     1861 gcctggatcc gcccgctggc cgggggcacc ccggtcaagg tgatgggcgg ccagaccggc
     1921 ggcgcggcca tcgccgacaa tgccttcccg gttgtgctgg aaacgcccgg cagcccgttt
     1981 gttggcggca tcgccggcgg cgcgcgggtc gattacgaga ttttccttgc gacccggacg
     2041 tcgctgctgg ccggcaagcc ggaatggcag cgccttgcga gctatgacga caagatcggg
     2101 ggcggggcgg tgcgcggcac cacggcctat ctgctcagcg ccaagaccaa cccggcccgc
     2161 gacgtgctgg ttcgcccgct cgatgccaag ggccggcctg gcgcggcaac ggtgctgatg
     2221 aagggcaacg accggctgat cctgaccggg atcaccgcgg cccgggacgg gatctacatt
     2281 tcggccacca ccgatggcgc cgggcggttg ttcttcaccc cgggcggaaa ggccgccgtg
     2341 cgcgaggtga agctgccctt cgaaggatcg atcctgggcc cggatgcgca gccggatggc
     2401 aacggcgtct ccttcgggct gaccggctgg accaacaaca tgaccagctt caccgcgcgc
     2461 ggcggcgtgg tccgggccac cgggatcgcc tcgcaaagct ggtcgggcgc cagcgggatc
     2521 ggggttcagc ggctggaggc ggtcagcgcc gacggcaccc gggtgccgct ggtggtgctg
     2581 cggagcaagg ggaccagcgg gccggtgccg acccttgtcg aggcctatgg cggctacggc
     2641 atcgatacgg cggcgcccta ttatgaccgc aacggcatgg cctggctggc caagggcggg
     2701 gccatggcct tttgcggggt gcgcggcggc ggagagcgcg gccggtcctg gcacgagggc
     2761 gggcgcggtc cgaacaagcc acgcgggatg gaggacctga tcgcctgcgc cgaaaccctg
     2821 accgccaagg ggatcgcccc cgcgcgcggc ccggtggcga ccggtgcctc gatggccgga
     2881 acgctggtgc cgaccgccgt cttgcgcaag ccttcggcct tcggcgggat gatcacccag
     2941 gtcggcgtgg tcaatccggt ccggatcggc caggcggaaa acggcgccaa ccagtttgcc
     3001 gagatcggtg acccttcgga tccgaagcag tttgccgatc tggtggcgat ggatgcctat
     3061 catatgatcc ccaaggccgc gggcctgccg cccagcctga tggtgatcgg gatgaacgac
     3121 cgccgcgtgg tgccctggat gacggccaag ttcgtcgccc gggcgcaggc caagtggccc
     3181 ggcacggtgt ggctgcgcgg cgatgacaag gccgggcacg gcgtgggcac gaccgaggag
     3241 gtgcggcgca gcgagtgggc cgatatctac gctttcgcct ggtggacgca gacgaagtag
     3301 gagcctcagc cgcgcgcttc ggtgtggccc agcgggccgc tgccggcgcc aaagccgggg
     3361 gcggcgcgca gggcggagcg gacgaacagc cgcgcccggt ccaccgcttc gggcagctcc
     3421 agcccgcgtc ccagataggt cgcgatcgcg ctggacaggg tgcagccggt gccgtgggtg
     3481 tggcgggtgt ggatccgggt ggcggccatg acgtgcggtt cctcgccggg gcggcacagc
     3541 acgtcctcca cccgctcgcc ctcggcatcg ccgcccttgg ccagataggc aatgccgcgc
     3601 gcggccatgc cctcgtgccc gcccagcgct tccagttcgg ggacgttggg ggtggtcagc
     3661 acggccagct gcatcagccg ttcaaacgcc gcgatggtct ccgcgtcggc cagcgccgcg
     3721 ccgctggtgg cgatcatcac cggatcgaac accaccggca cgccgagccg ctccagccgc
     3781 tccgccacca cggcggcgat cgcaggggag ccgagcatgc cgatcttgac cgcatcgacg
     3841 ccgatgtcgc tgacgcaggc atcgatctgc gccccgatca gctcggggct gagcacttcg
     3901 accgcggtga tccccagggt gttctgcgcg gtgatcgcgg tgatcgcggt catcgcatag
     3961 ccgcccagca tggtgatcgt cttgatgtcg gcctgaatgc ccgcgccgcc ggaactgtcg
     4021 gacccggcga tggagaggat gcggggcggg gccatcagcc cttgaacttc cgcgccgtgc
     4081 tgggcgggtg cttggcgtgg agcgccttgg cccgggtcgg ccggtccatc agctcgccgc
     4141 cgcaattggg gcagcgttcg tccaggccat cggcgcattc ggtgcagaag gtgcattcga
     4201 agctgcagat gaaggcaccg ggcagatcgg cgggaaggtc cgtgccgcac ttctcgcaat
     4261 cggggcgcat ctcaagcact ggcggcttcc ttcacagcat cgcagatcgc atcgacgacc
     4321 tgttcgactt cacctgcat
//
LOCUS       DASUDV010000346         7561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1115797,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000346 DASUDV010000000
VERSION     DASUDV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115797"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFV30_07620"
     CDS             <1..199
                     /locus_tag="VFV30_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX4847995.1"
                     /translation="LAYRVSNIYETCAALMAAGHTIHRPPRDGHMAFVKTPDGISVEL
                     LQDGTLPPQEPWASMPNTGSW"
     gene            complement(203..460)
                     /locus_tag="VFV30_07625"
     CDS             complement(203..460)
                     /locus_tag="VFV30_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847996.1"
                     /translation="MSERPPWFGKYWVPRRGRFRYIPIVWQGWAALLGAIAAPHCVWL
                     IPADLWPESLLRLPAAIAVLLVGLALLFRLARARSVRIDTD"
     gene            complement(457..1641)
                     /gene="nhaA"
                     /locus_tag="VFV30_07630"
     CDS             complement(457..1641)
                     /gene="nhaA"
                     /locus_tag="VFV30_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX4847997.1"
                     /translation="MKRRHPVSLAASAVIRLFGGEAGAGVLLITVAVLAMVAANSPLA
                     HGYHALFHGPLGWSPIAKLNTLHLWINDALMALFFFVVGLEIKREVLDGELASAERRR
                     LPVLAAAAGMAVPALTYLAVAGGEPGLGAGWAIPAATDIAFALGVLALLGKRVPPSLR
                     LFLLTVAIVDDLGAVVIIALFYTASLKLGWLALAAALLGAMAMLNRRGVANGWAYGLL
                     GAALWYAVLHSGVHATVAGVLAALTVPMRLDRMGDSPLLRMEHALAPLSAYIVVPVFG
                     FANAGVSLAGLSLADVAAPLPLGIALGLFLGKQAGIFGAIWTARRLRLAELPEGANWL
                     HLWGMAILCGIGFTMSLFIAQLGFPGNAVLVDEAKLGILLGSLLSAVAGYGVLRLAAA
                     RP"
     gene            1888..3306
                     /locus_tag="VFV30_07635"
     CDS             1888..3306
                     /locus_tag="VFV30_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEX4847998.1"
                     /translation="MAGAASALQPWLEWLLLFEHELLAFAAFWLCVGLADELAVDLAW
                     IWLRLTGRARTTALPVGYGAEPLSGRAAVLIPAFQEAPVIGTTLAHLLAAWPQRDLTV
                     YVGCYRNDPATLDAASRAAGSDPRVRIVVHSAAGPTTKADCLNRLYRALEVDEARSGR
                     SYASVLLHDAEDMVHPAALQAIDAALVLRDFVQLPVRPELPAGSKWIAGHYADEFAES
                     HAKTMVVRAALGAPLPAAGVGCGFSRSAMAELADRRAAAGEAGPFAPECLTEDYELGL
                     VLSKGGRGSEFLRLRDDAGTLVATGSFFPTELDAAVRQKTRWIHGIAFQGWDRMGWGS
                     GLVELWMALRDRRGPLTALVLAAAYLLLLVDAVVLALGLAGFDTSQTLSTPLRVMLYI
                     SLVGFVWRAAMRCAFTTREYGLRQGLLSVLRIPVANVVAIMAGRRALVAYLRSLRTGK
                     VVWDKTAHEGHPAAAYLRRVKA"
     gene            3303..4439
                     /locus_tag="VFV30_07640"
     CDS             3303..4439
                     /locus_tag="VFV30_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4847999.1"
                     /translation="MRLPSRSTRRGQPLTALAIILVIWVGARTALWAGSRAPEEAAAA
                     SEAGKAAPSAGTPAGTTQGGSTGPALQGPATAPVAPQTEHALPPLVAPETEAPRDPQT
                     VPPRIAGGHQLLLNMALSGDMPQPDQAAATTPLAPAPASPPVPYLPHAGEQALRWSAD
                     GWILWREGGNGFNLPGAGLPGASLPSGSYGSSQAGFILRYRLAPDSPLRPTLYLRASS
                     GLYQPRGEELAAGLALRPVRQLPVALMAEARVTRTPSGTVVRPAAALVSEVPPVSLPL
                     GFRAETYAQAGWVGGKDHTPFFDGQARIDRPVARSGKTELRLGAGAWGGAQRGANRLD
                     IGPSATLDLPVGPARTRLSADYRFRVAGDAAPGSGVAATFSAGF"
     gene            4492..5406
                     /locus_tag="VFV30_07645"
     CDS             4492..5406
                     /locus_tag="VFV30_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4848000.1"
                     /translation="MDVYLPIANLAVNGLVIVALGALTGILSGMFGVGGGFLTTPLMI
                     FYGIPPTVAAASAASQVTGASVSGVFAHTRRGGVDYQMGAVLVAGGLIGTLIGAGLFR
                     LLQELGQIDVVINLLYVLLLGGIGGVMARESWQALQAQKTGVPVPARKRRHHPMVASL
                     PLRWRFYRSGLYISPLAPLLLGVFTGILTMLMGIGGGFVLVPAMLYILGMSASVVVGT
                     SLFQILFVTMATTMMHAMTTKAVDIVLAALLLLGSVSGAQIGAQLAEKIPAERLRFVL
                     AALVLMIALRMALGLGWRPDEIYTVTPS"
     gene            5403..6155
                     /locus_tag="VFV30_07650"
     CDS             5403..6155
                     /locus_tag="VFV30_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX4848001.1"
                     /translation="MRILLALLALFTLVGARDPILVPEVSQHEIQVRQGFRGTELLLF
                     GAILTPEGTRAGQDYDIVVVLKGPTRSIELREKQRIAGIWINASSTEFRSAPTFFAVA
                     SSRPIKQIVDDKTAAIYELGLPWLQLSPIGAYDPQEQARFSAGLVDLMKREGLYKDDP
                     KGVKVSEQVLYQARIALPSSVQTGTYTAETFAVSKGRVVASAISRVEVKKLGFERAVA
                     QFAQYNGFFYGLLAVALSIAMGFLAGRLFALV"
     gene            6255..>7561
                     /locus_tag="VFV30_07655"
     CDS             6255..>7561
                     /locus_tag="VFV30_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4848002.1"
                     /translation="MNARGFGAGRPASAEEIPADAAQAAGQFDNAREPIGIVLEIAGS
                     GSAIALDLQRLQECSEDLDPSIALAGQVGSQVKIRVGKGWLLASVRTQRQDAKTPGGI
                     MAQIDFLGEGDEERLTGRIHSFRRGVTRYPIPGALIYPATSNDLRQIYASDGRSSIQV
                     GTVFPTRDIRAGLYVDAMLGKHFALLGSTGTGKSTSAALILHRICEHAPEGHIVMIDP
                     HGEYSAAFKTTGMIFDVSNLNMPYWLMNFEEHCEVFLTSSGNDRQEDMDILGKCLLAA
                     RSKNRLAESMGKITVDSPIPYLLSDLTNLIQTEMGKLDKATSSAPYMRIKNKIDELKA
                     DPRFQFMFSGMLVGDTMAEFIAKIFRMPSRGKPISIIDVSGVPSDITSTVVAVLSRMV
                     FDFAIWARDEKTRPVLLVCEEAHRYVPNEKNADGSSVGRILSR"
BASE COUNT         1170 a         2645 c         2559 g         1187 t
ORIGIN      
        1 tctggcctac cgggtgagca acatctacga aacctgcgcc gcgctgatgg cggcggggca
       61 taccatccac cgccctccgc gcgacgggca catggccttc gtcaagacgc cggacggcat
      121 ctcggtcgaa ctgctgcagg acggcactct cccgccgcag gagccctggg cgagcatgcc
      181 gaacaccggc agctggtagc gttcagtcgg tatcgattcg aacgctgcgg gcccttgcca
      241 agcggaacag caaggccagc ccaaccagca gcacggcgat cgcggcgggc agcctgagca
      301 agctttccgg ccacaggtct gccgggatca gccagacgca atgcggggcg gcaatggcac
      361 ccagcagcgc ggcccagccc tgccagacaa tcggtatgta gcggaaccgc ccgcgccgcg
      421 gcacccagta cttgccgaac cagggcgggc gctcgctcac ggccgggccg cggcaaggcg
      481 cagcacgcca tagcccgcca cggccgaaag cagcgaaccc agcaggatgc ccagcttcgc
      541 ctcgtccacc agcacggcgt tccccggaaa gcccagttgg gcgatgaaca ggctcatcgt
      601 gaagccgatg ccgcacagga tcgccatgcc ccacaggtgc agccagttgg ctccctccgg
      661 cagttcggcc aggcgcaggc gccgggcggt ccagatcgcg ccgaagatgc cggcctgctt
      721 gcccaggaac agccccagcg cgatccccag cgggagcggc gcggcgacat cggccagcga
      781 gagcccggcg agcgaaaccc cggcgttggc aaagccgaac accggcacca cgatataggc
      841 gctgagcggc gccagcgcgt gctccatccg cagcagcggg ctgtcgccca tccggtcaag
      901 ccgcatcggc acggtcagcg cggccagcac gccggccacg gtcgcgtgga cgccggaatg
      961 gagcaccgca taccacagcg ccgcgcccag caggccgtag gcccagccgt ttgccactcc
     1021 gcggcggttc agcatcgcca tcgcgcccag cagcgcggcg gccagggcca gccagcccag
     1081 cttgagcgag gcggtgtaga acagcgcgat gatcaccacc gcgcccagat cgtcgacgat
     1141 cgccacggtc agcaggaaca gccgcagcga cggcggcacc cgcttgccga gcagggccag
     1201 cacgcccagg gcaaaggcga tgtcggtggc ggccgggatc gcccagccgg cgcccagccc
     1261 cggttccccg ccggccacgg ccagataggt cagcgccgga acggccatgc cggccgcggc
     1321 ggccagcacc ggcaggcggc ggcgctcggc gctcgccagc tcgccgtcca gcacctcgcg
     1381 cttgatctcc agccccacca cgaaaaagaa cagcgccatc agcgcatcgt tgatccacag
     1441 gtgcagggtg ttcagcttgg caatcgggct ccagcccagc gggccatgga acagcgcgtg
     1501 atagccgtgg gccagcggcg aattggccgc caccatggcc agcacggcca cggtgatcag
     1561 cagcacgccc gcaccggcct ctccgccgaa cagacggatc acggccgagg cggcaagcga
     1621 tacgggatga cggcgcttca tggtttgccg gatagcccaa ccggcccggc aggcaagcaa
     1681 aaccggacag cccgggtgtg gcctcttcgg gccgctgcgg tgcgcctatt gattcgcttt
     1741 actttcagca gtgcaagtaa tgatcgcggg cgcaaataat cagggacggc ttgattggcg
     1801 cagcaacaat aacccgcgtt gcatggtccc gatcttcgcg ctaggcttcg cgctttgaca
     1861 cctacagcag gatcgatcgc acagggaatg gccggggcag catccgcctt gcaaccgtgg
     1921 ctcgaatggc tcttgctgtt cgagcacgaa ctgctggcct ttgccgcgtt ctggctgtgc
     1981 gtgggcctgg ccgatgagct ggcggtggac ctggcctgga tctggcttcg gctgaccggg
     2041 cgggcgcgga ccacggcgct gccggtcggc tatggggcag agccgctgtc cggacgcgcc
     2101 gcggtgctga tcccggcctt tcaggaagcc ccggtgatcg gcaccacgct ggcccacctt
     2161 ttggcggcct ggccgcagcg cgatctgacg gtctatgtcg gctgctaccg caacgatccg
     2221 gccacgctgg atgcggccag ccgggcggcg gggtctgacc cgcgggtgcg tatcgtggtg
     2281 cacagcgccg ccgggccgac caccaaggcc gattgtctca accggctcta ccgcgcgctg
     2341 gaagtggacg aggcccgttc gggccgttcc tatgcctcgg tcctgctcca cgacgcggaa
     2401 gacatggtgc atcccgccgc gctgcaggcg atcgacgcgg cgctggtgct gcgcgatttc
     2461 gtgcagctgc cggtccgtcc ggaactgccg gcgggatcga aatggatcgc cggccactac
     2521 gccgatgaat tcgccgaaag ccacgccaag accatggtgg tgcgcgccgc gctgggcgcg
     2581 ccgctgccgg cggccggggt gggctgcggc ttttcgcggt cggccatggc cgaactggcc
     2641 gatcgccgcg ccgccgccgg agaggccgga cccttcgccc cggaatgcct gaccgaggat
     2701 tacgagctgg gcctggttct ctccaaaggc gggcgcggca gcgagtttct gcggctgcgc
     2761 gacgatgccg ggacactggt ggcgaccgga tcgttcttcc cgaccgaact ggacgcggcg
     2821 gtgcggcaaa agacccgctg gatccacggg atcgctttcc agggctggga ccggatgggc
     2881 tggggttccg gcctggtcga gctgtggatg gccctgcgcg accggcgcgg cccgctgacc
     2941 gcgctggtcc tggccgcggc ctatctgttg ctgctggtcg atgccgtcgt gctcgccctt
     3001 ggccttgccg ggtttgacac gtcccagacc ctgtccaccc cgctgcgggt gatgctctat
     3061 atcagcctgg tcgggttcgt ctggcgggcc gccatgcgct gtgccttcac cacccgcgaa
     3121 tacggcctgc gccagggcct gctttcggtg ctgcgcattc cggtggccaa cgtcgttgcg
     3181 atcatggcag ggcgccgcgc gctggtggcc tatctgcgct cgctgcggac cggcaaggtg
     3241 gtgtgggaca agaccgccca cgaagggcat cccgccgcgg cctatctgcg gagggtcaag
     3301 gcatgaggct gccttcacgc tccacccgcc gcggccagcc gctgaccgct ctcgccatca
     3361 tcctggtgat ctgggtcggc gcccgcaccg cgctgtgggc cggatcgcgc gcgccggaag
     3421 aggctgccgc cgcaagcgaa gcgggcaagg ccgcgccgtc cgccggcacg cccgccggaa
     3481 ccacgcaagg cggctccacc ggtccggcgc tccaggggcc ggcgaccgcg cccgtagccc
     3541 cgcagaccga acacgccctg ccgccgctgg tcgcgcccga gaccgaggca ccgcgcgatc
     3601 cgcagaccgt gccgccgcgg atcgcgggcg ggcaccagct gctgctcaac atggcgctgt
     3661 cgggcgacat gccccagccc gatcaggccg ccgcaaccac tccgcttgcc ccggccccgg
     3721 ccagcccgcc ggtgccctat ctgccccatg ccggcgaaca ggccctgcgc tggtcggcgg
     3781 acgggtggat cctgtggcgc gaaggcggca acggtttcaa cctgccgggg gcgggcctgc
     3841 ccggggcgag cctgccttcg gggtcctatg gctccagcca ggccggcttc atcctgcgtt
     3901 accgcctggc gccggacagc ccgctgcgcc ccacgcttta cctgcgggcc agcagcgggc
     3961 tctaccagcc gcgcggcgag gagctggccg ccgggctggc gctgcgcccg gtccgccagc
     4021 tcccggtcgc gctgatggcc gaggcgcggg ttacccgcac gcccagcggc acggtggtgc
     4081 ggccggccgc ggcgctggtc tccgaagtgc ccccggtctc gctgccgctg ggcttccggg
     4141 ccgaaaccta tgcccaggcc gggtgggtcg gcggcaagga tcacacgccg ttcttcgacg
     4201 gacaggcccg gatcgaccgg ccggtggcgc gcagcggcaa gaccgaactg cgccttgggg
     4261 ccggggcctg gggcggggcc cagcgcgggg cgaaccggct cgatatcggc cccagcgcca
     4321 cgctggatct gccggtcggc ccggcgcgga cccggctctc ggccgactac cgtttccggg
     4381 ttgcgggcga tgccgcaccg ggttcggggg tggcggcaac cttttctgcg ggtttctagg
     4441 acctccggcc tttttttggc cttgcgggtg ggctagtccc gtttccacga gatggacgtc
     4501 taccttccga ttgccaacct tgcggtcaac ggcctggtca tcgtcgcgct tggcgcgctg
     4561 acgggtatcc tctcgggcat gttcggcgtg ggcggcgggt tcctgaccac gccgctgatg
     4621 atcttctacg gcattccgcc gacggtcgcc gcggcatcgg ccgccagcca ggtgaccggc
     4681 gccagcgttt ccggcgtctt cgcccacacc cgccgcggcg gggtcgatta ccagatgggc
     4741 gcggtgctgg tcgccggcgg gctgatcggc accctgatcg gggcggggct gttccgcctg
     4801 ctgcaggaac tcggccagat cgatgtggtc atcaacctgc tctacgtcct gttgctgggc
     4861 gggatcggcg gggtgatggc gcgggaaagc tggcaggcgc tgcaggcgca gaagaccggg
     4921 gttccggtcc cggcccgcaa gcgccggcac cacccgatgg tcgccagcct gccgctgcgc
     4981 tggcgctttt atcgttcggg gctctacatc tcgcccctgg cgccgctctt gctgggggtc
     5041 ttcaccggga tcctgaccat gctgatggga atcggcggcg gtttcgtgct ggttccggcc
     5101 atgctctaca tcctggggat gagcgccagc gtggtggtcg gcacctcgct gttccagatc
     5161 ctgttcgtga ccatggcaac caccatgatg cacgcgatga cgaccaaggc ggtcgatatc
     5221 gtgctggcgg cgttgctgct gctcggctcg gtctccggcg cgcagatcgg ggcccagctg
     5281 gccgagaaga tcccggccga gcggctgcgc ttcgtgctgg ccgcgctggt cctgatgatc
     5341 gcgctgcgca tggcgctggg gctgggctgg cgcccggacg agatctacac ggtgaccccg
     5401 tcatgaggat cctgctcgcc ctgcttgcgc tgtttaccct ggtcggggcc cgggatccga
     5461 tcctggtgcc cgaggtgtcg cagcacgaaa ttcaggtccg ccagggcttt cgcggcaccg
     5521 aactgctgct gttcggcgcg atcctgacgc ccgaggggac ccgggccggg caggattacg
     5581 atatcgtcgt ggtcctgaag gggccgaccc gctcgatcga gctgcgcgag aagcagcgga
     5641 tcgccgggat ctggatcaac gcctccagca ccgagttccg ctcggcgccg accttcttcg
     5701 cggtcgcgtc ctcgcggccg atcaagcaga tcgtcgatga caagaccgcc gcgatctatg
     5761 agctgggcct gccctggctg caactctcgc cgatcggggc ctatgacccg caggaacagg
     5821 cccgcttttc ggccggactg gtcgacctga tgaagcgcga agggctctac aaggacgatc
     5881 ccaagggcgt gaaggtctcc gaacaggtgc tctaccaggc ccgcatcgcc ctgccgtcca
     5941 gcgtccagac cggcacctac accgccgaaa ccttcgccgt ttcaaagggg cgggtggtgg
     6001 cctcggcgat cagccgggtg gaagtgaaga agctgggctt cgaacgcgcg gtggcgcaat
     6061 tcgcgcagta caacggcttt ttctatggcc tgctggccgt ggcgctctcg atcgcgatgg
     6121 gctttctggc cgggcgcctg ttcgcgctgg tctagcctgc caagccttag acttagccca
     6181 attttaacgc ttctcgtcta ccccgtttcg gtccgcaact ggcggaaaac agggacgcat
     6241 tggcgatgaa cgatatgaat gcacgcggtt tcggggcggg acgtccggca tcggcggagg
     6301 aaattccggc ggatgcggct caggccgcgg ggcagttcga caacgcccgc gaaccgatcg
     6361 gcatcgtcct ggaaatcgcc ggttcgggct cggccatcgc gcttgacctg cagcgcctgc
     6421 aggaatgctc cgaagacctc gatccctcga tcgcgctggc cggccaggtc ggcagccagg
     6481 tcaagatccg ggtcggcaag ggctggctgc tcgcctcggt ccgcacccag cgccaggacg
     6541 ccaagacccc cggcgggatc atggcccaga tcgacttcct gggcgaaggc gatgaagagc
     6601 gcctgaccgg ccgcatccac tcgttccgcc gcggtgtgac gcgctatccg attcccggcg
     6661 cgctgatcta tcccgccacc agcaacgacc tgcgccagat ctacgcctcg gacgggcgca
     6721 gctcgatcca ggtcggcacc gtcttcccga cccgcgacat ccgcgccggg ctctatgtcg
     6781 atgccatgct gggcaagcac ttcgcgctgc tcggctcgac cggtaccggc aagtcgacga
     6841 gcgccgcgct gatcctccac cggatctgcg aacatgcccc cgaagggcac atcgtgatga
     6901 tcgacccgca cggcgaatat tcggcggcgt tcaagaccac cggcatgatc ttcgacgtgt
     6961 cgaacctcaa catgccgtac tggctgatga acttcgaaga acactgcgaa gtgtttctga
     7021 cctccagcgg gaatgaccgc caggaagaca tggacatcct cggcaagtgc ctgctcgccg
     7081 cgcggtcgaa gaaccgcctg gccgaaagca tgggcaagat caccgtcgac agcccgattc
     7141 cctatctgct gtcggacctg accaacctga tccagaccga gatgggcaag ctcgacaagg
     7201 cgacctcctc ggcgccctat atgcggatca agaacaagat cgacgaactg aaggccgatc
     7261 cgcgcttcca gttcatgttc tccggcatgc tggtcggcga tacgatggcc gaattcatcg
     7321 ccaagatctt ccgcatgccc agccgcggca agccgatctc gatcatcgac gtctcgggcg
     7381 tgccgtccga cattaccagc accgtggttg ccgtgctcag ccgcatggtc ttcgacttcg
     7441 cgatctgggc ccgcgacgag aagacgcggc ccgtgctgct ggtctgcgaa gaagcccacc
     7501 gctatgtgcc gaacgagaag aacgccgatg gctcgtccgt cggccggatc ctcagccgga
     7561 t
//
LOCUS       DASUDV010000347         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_430911,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000347 DASUDV010000000
VERSION     DASUDV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430911"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2416
                     /locus_tag="VFV30_07660"
     CDS             <1..2416
                     /locus_tag="VFV30_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848003.1"
                     /translation="LRLGGQDLALRREGNTWRGESRVENARMQGGLSLRGVRQPGQML
                     ITRHANGKRFFTILGQAEAAAPAKTGGRLRIYWDRSRSHQGKAAADEAEALVALAEQT
                     RPEAIDLVTFASNAPQVTTLADAAALREALGKVTYRGATSLAGLDNLKLPDARRCVLV
                     SDGQVTLDRAAPFRPDCRLSTLSADSGADGARLGRLAQRQGGQFVRIAPGKGREAATA
                     LSDSGVSVIAVTSEASGRIGWRAFSQADGGWMVVGEMPFDTRLPSAAPVTVRVVSPSG
                     RISEESFAPDMAALPLDAGGALWAATRVGELADDPAQHEAMVALSRAFQVASPAMAFL
                     VLETPEQYLNADIAPPRGFPEEWMEEYKEAKTSRDEDKQTARADRLETVLELWGERKA
                     WWNTRFPLPRSKGGANGQQEAVPVGAPPPPPSPPPPPVLNRPSLESRETVSETAEEES
                     GGNDVLVVTGTRISNPGLQSATPVSAVSGQDLDAQVEIKLETVLADRPYLKALDAAAP
                     EARLRVLAEQEAEYGSVPAFYFDTADWFRLKGDAATARELMLSALELDKADNETLQIV
                     AFRLERDGEYDLAVQLAERLAVGAKFLPQPRRALALALAARGTAAGKNGRADLERAFA
                     LLVEVALEPTDSGEYDGLEVIALMEANALIPRIEALGGTWKLDRRLVGLFDTDARIVI
                     EWTADDADIDLWVDEPGGERVMYSNKQSDFGGQISNDMTDGYGPEEYAIRRAPKGEYR
                     VRVNGYDADRINPNGPGHVLVRLIRNHGRRSEKAVLVDVDLSFQEGPNRDQAEKTRPI
                     AVMTVN"
     gene            2528..2782
                     /locus_tag="VFV30_07665"
     CDS             2528..2782
                     /locus_tag="VFV30_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848004.1"
                     /translation="MGEDAATKESFGVLVGWAHHAFGNRLDLTLQCVDSTRRADRREI
                     DDHHIVMTPQQAAVLANYLYQISGQTPPEPRKGLLRRLLG"
     gene            2906..>4036
                     /locus_tag="VFV30_07670"
     CDS             2906..>4036
                     /locus_tag="VFV30_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX4848005.1"
                     /translation="MATTPTGTEAPTLTLTPPEPVPVIPTEKAVGLVPVAPEAKSKLE
                     EKVDAFVADLIASDANSPEFGKKVDQITNMGRKEIMAAAGMSNRFLDRPIRAMDKDSG
                     VGANLAELRRTVETLDPGKQGKLSKGRKFLGIIPFGNSMKRYFQSYESAQGHIQAVLG
                     HLSSGKDELLMDNAAIDVERQKLWEAMGNLEQMIHISRTLDGKLEEKAADLDATDPAK
                     AKAIRESALFYVRQRTQDLLTQMAVTVQGYLALDLVKKNNVELVKGVDRASTTTVAAL
                     RTAVTVAQAMANQRLVLQQVTALNETTAGLIDSTGTLLRDQTGKIHEMAASSTIPLET
                     LQRAFQNIYDTMDNIDTFKVKALENMKQTVTTLTAEVEKSKGY"
BASE COUNT          729 a         1355 c         1396 g          556 t
ORIGIN      
        1 attgcggctg ggcgggcagg atctggcgct gcgccgcgaa ggcaacacct ggcgcggcga
       61 aagccgggtg gagaacgccc ggatgcaagg cgggctgagc ctgcgcggcg tgcggcaacc
      121 ggggcagatg ctgatcaccc gccacgccaa cggcaaacgc tttttcacga ttctgggcca
      181 ggccgaggcc gccgccccgg ccaagaccgg cggacggctg cggatctatt gggaccggtc
      241 gcgctcgcac cagggcaagg ccgcggccga cgaggccgag gcgctggttg ccctggccga
      301 gcagacccgg cccgaagcga tcgacctggt gacctttgcc agcaacgccc cgcaagtgac
      361 caccctggcc gatgccgccg ccctgcgcga ggcgctgggc aaggtgacct accgcggcgc
      421 gacctcgctg gccgggctcg acaatctgaa gctgcccgat gcgcggcgct gcgtgctggt
      481 gtccgacggg caggtcacgc tggaccgcgc cgccccgttc cggcccgatt gccggctctc
      541 cacgttgagc gccgattcgg gcgcggacgg cgcgcggctg ggccggctgg cccagcggca
      601 gggcgggcag ttcgtgcgaa tcgcgccggg caagggccgc gaagccgcca cggcgctgag
      661 cgattccggc gtcagcgtca tcgcggtgac cagcgaagcc tctggccgga tcggctggcg
      721 cgccttttcc caggccgatg gcggctggat ggtggtgggc gaaatgccgt tcgacacccg
      781 cctgccctcg gccgcgccgg taacggtgcg ggtggtcagc ccgtccgggc ggatcagcga
      841 ggaaagcttt gcgcccgata tggccgctct gccgctggac gcaggcgggg cgctgtgggc
      901 ggccacgcgg gttggcgaac tggccgacga tccggcgcag cacgaggcga tggttgcgct
      961 gtcgcgcgcg ttccaggtgg ccagcccggc catggccttc ctcgtgctgg aaacgcccga
     1021 acagtatctg aatgccgaca ttgccccgcc ccgcggcttc cccgaggagt ggatggagga
     1081 atacaaggag gccaagacgt cgcgcgacga ggacaagcag accgcgcgcg ccgaccggct
     1141 ggaaaccgtg ctggagctgt ggggcgagcg caaggcctgg tggaacaccc gctttccgct
     1201 gccgcgcagc aagggcgggg cgaacgggca gcaggaggcc gtgccggtgg gcgcgccgcc
     1261 cccgccgccg tctccgccgc cgccaccggt attgaaccgc cccagcctgg agagccgcga
     1321 aacggtgagc gaaaccgccg aagaagagag cggcggaaac gacgttctgg tggtaaccgg
     1381 cacccgcatc agcaatcccg gcctgcagtc cgccacaccg gttagtgccg tgagcggaca
     1441 ggacctcgac gcccaggtgg agatcaagct ggaaaccgtg ctggccgacc ggccctatct
     1501 gaaagcgctg gacgcggcgg cgcccgaggc gcggctgcgc gtcctggccg aacaggaggc
     1561 cgaatacggc agcgttcccg cgttctattt cgacacggcg gactggttcc ggctgaaggg
     1621 cgatgccgcc accgcgcgcg agctgatgct ctccgcgctg gaactggaca aggccgacaa
     1681 cgagacgctg cagatcgtcg ccttccggct ggagcgcgac ggggagtatg accttgccgt
     1741 gcagctggcc gaacggctgg ccgtgggcgc caagttcctg ccccagccgc gccgggccct
     1801 ggccctcgcg ctcgccgcgc gcggcaccgc cgcgggcaag aacggccgcg ccgatctgga
     1861 gcgggccttt gccctgctgg tcgaggtggc gctggagccg accgacagcg gcgaatacga
     1921 cgggctggaa gtgatcgcgc tgatggaggc caacgccctg atcccgcgga tcgaggcgct
     1981 gggcgggacc tggaagcttg accggcggct ggtcggcctg ttcgacaccg acgcgcggat
     2041 cgtgatcgaa tggaccgccg acgacgccga catcgacctg tgggtcgacg agccgggcgg
     2101 cgagcgggtg atgtacagca acaagcagtc ggactttggc gggcagatct ccaacgacat
     2161 gaccgacggc tacgggcccg aggaatacgc catccgccgc gcgcccaagg gcgaataccg
     2221 ggtccgggtc aacggctatg acgccgaccg gatcaacccc aacgggcccg gccacgtcct
     2281 ggtccggctg atccgcaacc acgggcggcg cagcgagaag gccgtgctgg tcgacgtcga
     2341 cctgagcttc caggaaggac ccaaccggga tcaggcggaa aagacccgcc cgatcgcggt
     2401 gatgaccgtc aactagggcg cggtcccgcc gcgctccact tcgggacagc ctgcccggtt
     2461 gcattcgccg cgcagcgcaa tagtctgggc cccggtcgca ccgaacttgc cgctgtggag
     2521 gatggcgatg ggcgaggatg ctgcaacgaa agagagtttt ggcgtgctgg tcggctgggc
     2581 gcaccatgcg ttcgggaacc ggctggacct gacactgcaa tgcgtcgatt cgacccggcg
     2641 cgcggaccgg cgcgagattg acgaccatca catcgtgatg acgccccagc aggccgcggt
     2701 gctggccaac tatctctacc agatttccgg ccagaccccg ccggaaccgc gcaagggcct
     2761 gctgcggcgc ctgctgggct gacgccgctc gtcgacgcca ctctgtctta tgtcgacaat
     2821 acagcttgca tcgcgcgggc tgtgatccca cattggcatc accccgtttt tcggaccccg
     2881 ttttttagga tacggaacaa agccaatggc caccacgccg accggaaccg aagccccgac
     2941 gctgaccctg accccgcccg aaccggtgcc ggtgatcccc accgaaaagg ccgtgggcct
     3001 tgtcccggtc gcgcccgaag ccaagtccaa gctggaggaa aaggtcgatg ccttcgtggc
     3061 cgatctgatc gcgtccgatg ccaattcgcc cgaattcggc aagaaggtgg accagatcac
     3121 caacatgggc cgcaaggaaa tcatggccgc ggcgggcatg tcgaaccgct tcctcgaccg
     3181 gccgatccgg gcgatggaca aggattcggg cgtcggcgcc aacctggccg agctgcgccg
     3241 cacggtcgaa acgcttgatc ccggcaagca gggcaagctc tccaagggcc gcaagttcct
     3301 ggggatcatt cccttcggca attcgatgaa gcgctatttc cagtcctacg aaagcgcgca
     3361 gggccacatc caggcggtgc tgggccacct gtccagcggc aaggacgagc tgctgatgga
     3421 caacgccgcg atcgatgtgg agcgccagaa gctgtgggaa gccatgggca acctggagca
     3481 gatgatccac atttcccgga ctctggacgg caagctggag gaaaaggccg ccgatctgga
     3541 cgcgaccgac ccggccaagg ccaaggcgat ccgcgaatcg gcgctgttct atgtccgcca
     3601 gcgcacccag gacctgctca cccagatggc ggtgaccgtg cagggctatc tcgcgctcga
     3661 tctggtcaag aagaacaacg tcgaactggt caagggcgtg gaccgggcca gcaccaccac
     3721 cgtcgccgcg ctgcgcaccg ccgtaaccgt ggcccaggcc atggccaacc agcggctggt
     3781 gctgcagcag gtcaccgcgc tcaatgaaac caccgctggc ctgatcgatt cgaccggcac
     3841 gctgctgcgc gaccagaccg gcaagatcca cgaaatggcg gcctcgtcga cgatcccgct
     3901 ggaaacgctg cagcgcgcgt tccagaacat ctatgacacg atggacaaca tcgacacctt
     3961 caaggtgaag gcgctcgaga acatgaagca gacggtcacc acgctgaccg ccgaggtgga
     4021 gaagtcgaag ggctat
//
LOCUS       DASUDV010000348         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_608092,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000348 DASUDV010000000
VERSION     DASUDV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608092"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFV30_07675"
     CDS             complement(<1..200)
                     /locus_tag="VFV30_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848006.1"
                     /translation="MQNRKLLFALSISTIALAACAPQKERVASTAAPEPAWAFEKSDV
                     PVDPGYRFGRLPNGMRYVIRQNA"
     gene            complement(240..2270)
                     /locus_tag="VFV30_07680"
     CDS             complement(240..2270)
                     /locus_tag="VFV30_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX4848007.1"
                     /translation="MRSLIPVAAILACTAAVAESGDKEILFAPTPAWVQLAEPLPVPD
                     NPRGLLFVRRQNIVAHLDAKGETLFMSSLLRIVHPNALQLGNISFAWSPAAGKPVIHA
                     VKVHRNGTVRDVLATTKFEILRREDQLEAAMLDGRLTATLRVPDLRVGDDLELSYSIP
                     GSDPTLGKDSFGVLFLDPNPAPGRFVLQLSWEEGQEPKYLPTPDINSKITRGARSVTY
                     AEDMPAVLKPPKDAPPRYNWQRVLAYSDYADWQSVSTRFAPLFRKAATLAPNSPIKQE
                     AAEIAAAYADPRDRAAAALKLVQQQVRYVYVGFNGGNYTPASAEETWQRRYGDCKGKT
                     ALLLALLNELGIAAEPVLANNTGGDDGLDQRLPNPAFFDHVLVRATIGGQAYWLDGTL
                     PPVATPQTTPALPYRWVLPLKDKGAALEQIPWKPATRPDRLALFEIDARDGFDVPARR
                     RMVMITRGPEAIAAHNQMSAVTDEQMLSEFKRELEGTEDWISIDKVSWRFDGKAMASV
                     IEVSGTGKIDWKSIGRSRELFLPVGGYSPPYRRQRPADQDQTVPFYKAPEYDCRVVTV
                     RLPAMTAEKDWSFGTNYFVTYFGQTFLRSYERRDRSIRMVSSNRTLLTEIDPASAARD
                     NARLDKFDNSKARIFHDPNSADIKKPGFVVPATYEIDWLNDDSACMNPQPKP"
     gene            complement(2364..2918)
                     /locus_tag="VFV30_07685"
     CDS             complement(2364..2918)
                     /locus_tag="VFV30_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX4848008.1"
                     /translation="MALPAPADVVAFWRAAGPDKWFAKDDAFDAALAARFHDAHHAAA
                     RGELDGWQADGEGVLALILLLDQVPRNLWRGSAHAFATDPLARTVAARAVDTGLDSAV
                     DPALRVFLYLPFEHSEDLADQDRCVALCQALDAESGSEWTRWAVMHRDIVERFGRFPH
                     RNQCLGRTSTAAELAFLAAGGFAG"
     gene            complement(2918..>3344)
                     /locus_tag="VFV30_07690"
     CDS             complement(2918..>3344)
                     /locus_tag="VFV30_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX4848009.1"
                     /translation="YYFKDDALGDPMIAEEHIACFGWASNEEMQDISAMAIRVNDFMC
                     GMFAAINIRLVDFKLEFGRLWDGDYSRIILADEISPDGCRLWDMVTGEKLDKDRFRRD
                     LGGEEEAYQEVARRLGLLDADNGPSEVFDLSAHRKLRGK"
BASE COUNT          547 a         1068 c         1138 g          591 t
ORIGIN      
        1 gcgttctggc ggatgacata gcgcatcccg ttgggcaggc ggccgaaccg gtagcccgga
       61 tcgaccggca cgtcgctctt ttcaaaggcc caggccggct ctggcgcggc cgtgctcgcg
      121 acccgctctt tctgcggagc gcaggcggcg agggcgatgg tggaaatcga aagggcgaac
      181 agcagcttgc ggttttgcat ggacgccttg ttgctgaggg accgggcctt cgcaagccct
      241 tacggcttgg gctgcgggtt catgcaggcg ctgtcatcgt tcagccagtc gatttcatag
      301 gtcgcgggga ccacgaaccc cggcttcttg atgtccgcgc tgttgggatc gtggaagatc
      361 ctggccttgg agttgtcgaa cttgtcgagc cgggcgttgt cgcgcgcggc gctggccggg
      421 tcgatctcgg tcagcagggt gcggttggaa ctgaccatcc ggatcgaccg gtcgcgccgt
      481 tcatagctgc gcaggaacgt ctggccgaaa taggtgacga agtaattggt gccgaacgac
      541 cagtccttct cggcggtcat ggcgggcagc cgcacggtga ccacgcggca gtcatactcc
      601 ggcgccttgt agaacggcac ggtctggtcc tgatcggccg ggcgctggcg gcggtagggc
      661 ggactgtagc cgccgaccgg caggaacagt tcgcgcgagc ggccaatcga cttccagtcg
      721 atcttgccgg ttcccgatac ctcgatcacg ctggccatgg ccttgccgtc gaaccgccaa
      781 ctgaccttgt cgatgctgat ccagtcctcg gttccttcca gctcgcgctt gaattcgctc
      841 agcatctgtt cgtcagtcac agcggacatc tggttgtgag cggcaatcgc ctcgggcccg
      901 cgggtgatca tcaccatccg gcgccgggcc ggaacgtcga atccgtcgcg ggcgtcaatc
      961 tcgaacaggg ccagccggtc tggccgggtt gccggcttcc aggggatctg ttccagcgcc
     1021 gcgcccttgt ccttcagcgg caggacccag cggtagggca gggccggcgt ggtctggggc
     1081 gtggccaccg ggggcagcgt gccatccagc caataggcct gcccgccgat cgtcgcgcgg
     1141 accaagacgt gatcgaagaa cgcgggattg ggcaagcgct ggtccagccc gtcatcgccg
     1201 ccggtgttgt tggccagcac cggttccgcg gcaatcccca gctcgttcag caaggccagc
     1261 agcagtgcgg tcttgccctt gcaatcgcca tagcggcgct gccaggtctc ctcggcgctg
     1321 gccggggtgt aattgccgcc gttgaagccg acataaacat agcggacctg ctgctgcacc
     1381 agcttcagcg ctgccgccgc ccggtcgcgc gggtcggcat aggcggcggc gatttcggcc
     1441 gcttcctgct tgatgggtga attgggggcc agcgttgccg ccttgcggaa cagcggtgcg
     1501 aaccgggtgg agaccgattg ccagtcggca tagtcgctgt aggccagcac ccgctgccag
     1561 ttgtagcgcg gcggggcgtc cttgggcggc ttgagcacgg ccggcatgtc ctcggcatag
     1621 gtcacgctgc gcgcgccacg ggtaatcttg gaattgatat cgggggtggg caggtacttg
     1681 ggttcctgac cctcttccca gctcagttgc aggacaaagc gccccggggc gggattgggg
     1741 tccagaaaca gcacgccgaa gctgtccttg cccagcgtcg ggtcggatcc ggggatcgaa
     1801 taggacagct ccagatcatc gccgacgcgc agatcgggca cgcgcagggt ggcggtcagc
     1861 cggccatcga gcatggcggc ttccagctga tcctcgcgcc gcaggatctc gaacttggtg
     1921 gtggccagga catcgcggac cgtaccgttg cggtgcacct tcaccgcgtg gatgaccggc
     1981 ttgccggccg ccgggctcca cgcgaacgag atgttgccga gctgcagcgc gttgggatgg
     2041 acgatccgca gcagcgaaga catgaacagc gtctcgccct tggcatcaag atgcgcgacg
     2101 atgttctggc ggcggacgaa caacaggccg cggggattgt ccggtaccgg caagggctct
     2161 gccagttgca cccaggccgg ggtcggagcg aacaggatct ctttgtcccc gctctcggca
     2221 accgccgcag tacaggccag aattgctgca acgggtatta aggaccgcat gtaattccta
     2281 cccctttgcg ggcaagagaa tgcgattttc cgtctcttgg caagcgtcta ttctgtgcag
     2341 acgcaagaat tgcgcgcggc cgttcagccg gcgaaaccgc ccgcggccag aaaggccagc
     2401 tccgcggcgg tgctggtccg gccgaggcac tggttgcggt gcgggaagcg gccgaaacgc
     2461 tccacgatat cgcggtgcat cacggcccag cgggtccatt cgctgccgct ttccgcgtcg
     2521 agcgcctggc acagcgcgac gcagcggtcc tggtcggcaa ggtcctcgga atgctcgaac
     2581 ggcagataga ggaacacccg cagcgccgga tcgaccgcgc tgtcgagccc ggtgtccacg
     2641 gcgcgggccg cgaccgtgcg ggccagcgga tcggtggcaa aggcatgggc cgatccgcgc
     2701 cacaggttgc gcggcacctg atcgagcagc aggatcagcg ccagcacgcc ttcgccatcg
     2761 gcctgccagc cgtccagctc gccgcgggcg gcggcgtggt gggcatcgtg aaaccgcgcg
     2821 gccagcgcgg catcaaaggc atcgtccttg gcaaaccact tgtccggccc ggccgcgcgc
     2881 cagaaggcaa cgacatcggc cggggcgggc agggccatta cttgccgcgc agcttgcggt
     2941 gcgccgaaag gtcgaacacc tcgctcgggc cgttgtcggc atcgagcagg cccaggcggc
     3001 gggcgacttc ctgataggcc tcttcctcac cgcccagatc gcggcggaag cggtccttgt
     3061 cgagcttttc gccggtgacc atgtcccaca ggcggcagcc atcggggctg atctcgtcgg
     3121 ccaggatgat ccggctgtaa tcgccgtccc acagccggcc gaattccagc ttgaaatcga
     3181 ccaggcggat gttgatcgcg gcaaacatcc cgcacatgaa atcgttgacc cggatcgcca
     3241 tggccgagat gtcctgcatc tcttcgttgc tggcccagcc gaagcaggcg atgtgctctt
     3301 ccgcgatcat cggatcgccc agcgcatcgt ccttgaaata gtat
//
LOCUS       DASUDV010000349         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_873075,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000349 DASUDV010000000
VERSION     DASUDV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873075"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VFV30_07695"
     CDS             complement(<1..1703)
                     /locus_tag="VFV30_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4848010.1"
                     /translation="MRLTALALLLSCMALASPVAAEVPGSTFSGEDLFKLESARDPQP
                     SPNGKLIAYVRQSGDVMSDRLRRSIWLIDPASGQQSPLVTGPGSYLSPRWSPDGTRIA
                     YIAANEDGAQLWVRWIATGTVARLTNLPDAPQSIAWSPDGKSIAYSMRVPEDGPRLGK
                     APAKPEGAKWAEPLEIYDRVTYRTDSGGYIRPGYNHLFLIFADGGAPRQLTFGAFDDD
                     GPLSWTPDGKAILFTSVRRPDWQREPFDSEVHQLDLASGTLTALTSRYGPDANPVASP
                     DGRMIAYLGFDDRHRSFQNTYLYVMNRDGSGARALTARHDLSIDEVQWAPDSKSLIVG
                     YDEKGAKKLARVGLDGSLRPVAAGLSGAGYDRPYTGGDYAVGRDGTIAWTAGSDQRPP
                     DVHALRAGKPAQLTRLNDGWITAKALAKVDKIPVTASDGRSIDAWLMLPPGRQPGQKV
                     PMILEIHGGPHSAYGPWFSTDYQLYTAAGYAVLYTNPRGSTSYGEAFAALIDRAYPGD
                     DYGDLMASVDSAIAGGAIDPDNLFVTGGSGGGVLTSWIVGKTDRFKAAATQKPVINWT
                     S"
     gene            1829..>2225
                     /locus_tag="VFV30_07700"
     CDS             1829..>2225
                     /locus_tag="VFV30_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX4848011.1"
                     /translation="MARSPAGTPPHPKGAERFDEGRAIDTVKAGQPDLAAGVAAIREV
                     VATLKPKPGVYRMLDARGDVLYVGKARVLRNRVANYTQVERLPIRLQRMVAQTRAMTI
                     VTTNSEAEALLLEAQLIKRFRPPYNVLLRD"
BASE COUNT          394 a          712 c          751 g          368 t
ORIGIN      
        1 tcgctggtcc agttgatcac cggcttctgc gtggccgcgg ccttgaagcg atcggtcttg
       61 ccgacgatcc acgaagtcag caccccgccg cccgatccgc cggtgacgaa caggttgtcg
      121 ggatcgatcg cgccgccggc aatcgccgag tcgaccgagg ccatcagatc gccatagtca
      181 tcacccggat aggcccggtc gatcagcgcg gcgaaggcct cgccatagga cgtgctgccg
      241 cgcggattgg tgtaaagcac ggcatagccg gccgccgtgt aaagctggta gtcggttgaa
      301 aaccaggggc cataggccga atgcgggccg ccgtggatct cgaggatcat ggggaccttc
      361 tggccgggct ggcgcccggg cggcaacatc agccaggcat cgatcgagcg gccatcgctc
      421 gccgtgacgg gaatcttgtc gaccttggcg agcgccttgg ccgttatcca gccatcgttc
      481 aggcgggtca gttgcgccgg tttgcccgcg cgcaaggcgt ggacatcggg tgggcgctgg
      541 tcgctgcccg ccgtccaggc gatcgtgcca tcgcggccca ccgcatagtc gccgccggta
      601 taggggcggt catagccagc accggagagg cccgcagcga ccggcctcag cgaaccgtcc
      661 agcccgaccc gggccagttt cttggcaccc ttttcgtcgt agccgacaat caggctcttg
      721 ctgtcgggcg cccattgcac ctcgtcgatg ctcaaatcgt ggcgggcggt cagcgcgcgg
      781 gcgccgctgc catcgcggtt catcacgtag aggtaggtgt tctggaagct gcggtgccgg
      841 tcgtcgaagc ccagataggc gatcatccgg ccatcgggag acgcgacagg attggcatcg
      901 gggccatagc ggctggtcag cgcggtcagc gtgccgcttg ccaagtcaag ctggtgcacc
      961 tcggaatcga agggttcgcg ctgccaatcc gggcggcgga ccgaggtgaa caggatcgcc
     1021 ttgccatcgg gtgtccacga aagcggacca tcgtcgtcga aggcgccgaa ggtcagctgg
     1081 cgcggcgcgc cgccatcggc gaaaatcagg aacaggtggt tgtagccagg acggatgtag
     1141 ccaccggaat cggtccgata ggttacccga tcatagattt cgagcggttc cgcccatttc
     1201 gccccttcgg gcttggccgg agccttgccc aggcgcgggc cgtcttcggg tacccgcatc
     1261 gaataggcga tcgacttgcc atcgggcgac caggcgatcg attgcggcgc atcgggcagg
     1321 ttggttagcc gggcaacggt gccggtggcg atccagcgga cccacagctg cgcgccatcc
     1381 tcgttcgcgg cgatataggc gatccgggta ccatcgggcg accagcgcgg cgaaagatag
     1441 ctgcccgggc cggtgacaag cgggctctgc tggccgctgg ccggatcgat cagccagatc
     1501 gaccggcgca gccgatcgct catcacatcg ccgctttggc ggacataggc gataagcttg
     1561 ccgttgggac tgggctgggg atcgcgcgcg gattcgagct tgaacaggtc ttcacccgaa
     1621 aaagtgctgc ccggaacctc tgcggcgacc gggctggcca gggccatgca ggacagcagc
     1681 aaggcgagag cggtcaggcg catctggatt ctccctgttc agacccagag gtcagcttgc
     1741 gaccgaacgg tactgaaagc aacgcggatt gtgcatgtcg cttgatcccc gggatgacct
     1801 ttcgaggcaa aacgactaga gccatcacat ggcccgcagt cccgccggaa ccccgccgca
     1861 tcccaaaggc gccgaacgct ttgatgaagg gcgtgcgatc gatacggtaa aggccggcca
     1921 gcccgatctg gccgcaggcg tagccgcgat ccgtgaagtg gtcgccacgc tcaagcccaa
     1981 gcccggggtc tatcggatgc tcgatgcccg cggcgacgtg ctctacgttg gcaaggcccg
     2041 ggtgctgcgc aaccgggtgg ccaattatac ccaggtcgaa cgcctgccga ttcggctaca
     2101 gcggatggtg gcccagaccc gggcgatgac gatcgtcacc accaattccg aagccgaggc
     2161 gctgctgctc gaagcgcagc tgatcaagcg cttccgcccg ccctacaacg tgctgctgcg
     2221 cgacg
//
LOCUS       DASUDV010000350         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1933391,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000350 DASUDV010000000
VERSION     DASUDV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933391"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFV30_07705"
     CDS             complement(<1..719)
                     /locus_tag="VFV30_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX4848012.1"
                     /translation="MARQFFGTDGIRGLTNAGVMTAAIAMKVGQAAGTRFLRGKHRHR
                     VVIGKDTRLSGYMIENALVAGFTSVGMDVVLTGPLPTPAIAMLTRELRADVGVMISAS
                     HNPYHDNGIKLFGPDGFKLSDEDELAIEAMLGQDLALAPAAEIGRARRIEDARGRYIH
                     AVKGSLPDDVRLDGLKIVVDCANGAAYQVAPSAMWELGAEIIAVGVSPNGVNINDKCG
                     STHLALVKETVVASGADIGIAL"
     gene            811..2055
                     /locus_tag="VFV30_07710"
     CDS             811..2055
                     /locus_tag="VFV30_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX4848013.1"
                     /translation="MTERQAASGFPEIRVPALWTFAALILGFVVASFLRGSAALDSLL
                     AVAEPMGSLWLRALQLTILPLVIGLVVTGISQTLAAAGGGALARRAVVLFVIVLLSAG
                     TMSALLVPAVLEMVPIPQQAVAALSGGAADPGKVPGVVDILNAMMPENIFAAASDGKM
                     LPVVIFAALFALALTRIGAVQRQAIMTFFEGLAGAMMVIVGWVLMLAPLGVFGLAVSL
                     GAQTGADAIGALAHYIVVVSLAGFVVLLAGYGIAVTLGRRRFVDFARAMAPVNAVAIS
                     TQSSLASLPAMLAATRKLGVREATADFVLPMAVAIFRATSPAMNMGVAIYAAYLTGTP
                     LSAAALMAGVFVAFLVSLSSVSLPGTISFVISVGPIAMAMGVPIAPLALLVAVEMLPD
                     IMRTLGNVTLDVAVTTAVDEDT"
     gene            complement(2204..>2910)
                     /locus_tag="VFV30_07715"
     CDS             complement(2204..>2910)
                     /locus_tag="VFV30_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4848014.1"
                     /translation="TVMMGHQPQRYQHYIEALESQPAKLLRTYDLDIVKPFPPLIQRI
                     VQSGHKNAKIRIREVDKSKFDDEAAIILHILNDAWSDNWGFVPFTDREIAYAGKKFKP
                     IVREDLIMIAEYEGEPVAFMMTLPDLNEVLKPLGGSLFPFGWAKLLWWLRKPRCRTMR
                     VPLMGVLKKHQSSRLASQLAFMMIEAIRQSATGRYGATRGEIGWVLDDNQGMNAIADA
                     IESTVNKEYTIYEKAL"
BASE COUNT          397 a          957 c          997 g          559 t
ORIGIN      
        1 agcgcgatgc cgatgtccgc gccgctggcg acgaccgttt ccttgaccag cgccaggtgg
       61 gtcgatccgc acttgtcgtt gatgttgacg ccgttgggcg agacgccgac cgcgatgatt
      121 tccgcgccca gttcccacat cgccgacgga gccacctgat aggccgcgcc gttggcgcaa
      181 tcgaccacga tcttgagccc gtccagtcgg acatcgtcgg gcagcgagcc cttgactgcg
      241 tggatatagc gcccgcgcgc gtcctcgatc cggcgggccc ggccgatctc ggccgccggg
      301 gcgagggcca ggtcctggcc aagcatcgcc tcgatcgcca gctcgtcctc gtccgacagc
      361 ttgaagccat cggggccgaa cagcttgatc ccgttgtcgt gataggggtt gtggctggcc
      421 gagatcatca cgccgacatc ggcgcgcagc tcgcgcgtca gcatggcgat ggcgggggtg
      481 ggcagcggcc cggtcagcac cacgtccatc cccacgctgg taaagccggc gaccagcgcg
      541 ttctcgatca tgtagcccga aagccgggtg tccttgccga tcaccacgcg gtggcggtgc
      601 ttgccgcgca ggaaccgggt gccggccgcc tggccgacct tcattgcgat tgcggcggtc
      661 atcacgcctg cgttggtgag cccgcggatc ccgtcggtgc cgaaaaattg ccgtgccatg
      721 cctgcctctt agcagcggat gccttgagaa attgctatgt cgcttcgcgg cgctaaagtc
      781 acgccttcaa acagggaccg gggagtaacc atgacagaac gccaggccgc atcgggcttt
      841 cccgaaatca gggttcccgc gctgtggacc tttgccgcgc tgatcctggg ctttgtcgtc
      901 gcctcgttcc tgcggggcag cgccgcgctc gattcgctgc tggccgtggc cgaaccgatg
      961 ggcagcctgt ggctgcgcgc gctgcagctg acgatcctgc cgctggtgat cggcctggtc
     1021 gtgaccggca tttcgcagac cctggccgcg gccggcggcg gcgccctggc gcgccgtgcg
     1081 gtggtgctgt tcgtgatcgt gctgctctcg gccgggacga tgtcggcgct gctggttccg
     1141 gcggtgctgg aaatggtccc gatcccgcag caggccgtcg cggcgctgag cggcggagcg
     1201 gccgatccgg gcaaggttcc gggcgtggtc gatattctga acgcgatgat gccggagaac
     1261 atcttcgccg ccgcttcgga tggaaagatg ctgccggtgg tgatcttcgc cgcgcttttc
     1321 gcgctggcgc tgacccggat cggcgcggtg cagcgccagg cgatcatgac cttcttcgaa
     1381 gggctggccg gggcgatgat ggtgattgtc ggctgggtgc tgatgctggc gccgctgggc
     1441 gtcttcggcc tggcggtttc gctcggcgcg cagaccgggg ccgacgcgat cggcgccttg
     1501 gcgcactata tcgtggtggt ttcgctggcc gggtttgtgg tgctgctggc cggctatggc
     1561 atcgccgtca cgctggggcg ccgccgcttc gtcgattttg cccgcgccat ggccccggtc
     1621 aacgcggtgg cgatctcaac ccagtcctcg ctcgccagcc tgccggcgat gctggccgcc
     1681 acgcgcaagc tgggcgtgcg cgaggcgacg gccgatttcg tcctgccgat ggcggtggcg
     1741 atcttccgcg cgaccagccc ggcgatgaac atgggcgtgg cgatctacgc cgcttatctc
     1801 accggcacgc cgctctccgc cgcggcgctg atggccgggg tcttcgtggc cttcctggtc
     1861 tcgctcagct cggtctcgct gcctggcacg atcagcttcg tgatctcggt cggcccgatc
     1921 gcgatggcga tgggcgtgcc gatcgcgccg ctggcgctgc tggtggcggt ggaaatgctg
     1981 cccgacatca tgcgcacgct cggcaacgtg acgctggacg ttgcggtgac cacggcggtg
     2041 gacgaggaca cgtaattcct ccccggcacg gggaggggga ccaccgaagg tggtggaggg
     2101 gcacgcgcgg gttatcccgc ggcctctccg gcgcggtaag gactgcttcc gcgtgcccct
     2161 ccaccatgct gcgcatggtc cccctccccc caagggggag gaattacagc gccttctcgt
     2221 agatcgtgta ttccttgttg accgtgcttt cgatcgcgtc ggcaatcgcg ttcatgccct
     2281 ggttgtcgtc cagcacccag ccgatctcgc cccgggtcgc gccgtagcgc ccggtcgcgc
     2341 tctgccggat cgcctcgatc atcatgaagg ccagctggct ggcgagacgg ctcgactggt
     2401 gctttttcag cacgcccatc agcggcaccc gcatggtccg gcagcgcggc ttgcgcagcc
     2461 accacagcag cttggcccag ccaaacggga acaggctgcc gcccagcggc ttcagcactt
     2521 cgttcagatc cggcagggtc atcatgaagg ccaccggctc gccctcgtac tcggcgatca
     2581 tgatcaggtc ttcgcggacg atcggtttga acttcttgcc ggcataggcg atctcgcggt
     2641 cggtgaaggg cacgaagccc cagttgtccg accaggcatc gttgaggatg tggaggatga
     2701 tcgccgcctc gtcgtcgaac ttcgacttgt cgacctcgcg gatccggatc ttggcgttct
     2761 tgtggccgga ctggacgatc cgctggatca gcggcgggaa cggcttgacg atatccaggt
     2821 cataggtccg cagcagtttg gccggctggg actccagcgc ctcgatatag tgctgataac
     2881 gctgcggctg gtggcccatc atcacggtcg
//
LOCUS       DASUDV010000351         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1933400,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000351 DASUDV010000000
VERSION     DASUDV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933400"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..357
                     /locus_tag="VFV30_07720"
     CDS             118..357
                     /locus_tag="VFV30_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX4848015.1"
                     /translation="MTLDQIPIGSSARIVAVDWPALVPEEALRLQALGIDAGARVAVD
                     HRGIFGTRDPIALAIGRMTVALRRAHARAIEVELL"
     gene            354..2192
                     /locus_tag="VFV30_07725"
     CDS             354..2192
                     /locus_tag="VFV30_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX4848016.1"
                     /translation="MTLRTAALVGNPNAGKSALFNALTGARQKIANYPGVTVERKAGR
                     LTLPSGEAIELTDLPGSYGLNATSPDEEVTRRVILGELPGEAAPQVLVVVLDASNLEQ
                     HLVFAQEVIALGRPTVVALNMVDLAERDGLVIDPAALEEALGVPVIATVAVRRRGLPE
                     LAAAIAAAEERAPEHHRPHTTLTERRVTAQAIAEAAIVSETSRHRLHARLDRLFLHPW
                     LGPMILFAMLFVVFQAVFTWATPFADALEGLIGLLSEQARAVLPEGLPRDLLTDGILS
                     GVGSVVVFLPQIVILFFFILAMEASGYMARAAFLMDRLMAGVGLSGRSFIPLLSSFAC
                     AIPGIMATRSITDPRDRLTTILVAPLMTCSARLPVYAVIIAAFIPNRSVLGPVGLQGL
                     VLLGLYVFGIVAAMLVALVLRRSVTKGAASGFIMELPKYQMPRLKDLLIGLWQRAWIF
                     LRRAGTIIFMVTVVLWVLLNFPRAGEGQNQVDASIAGKIANGLAVVVEPIGFNRDIAL
                     SLIPAMAAREVAVSSLATSYAVGADDEEAQAQELGERLKADWTLPTALAFLAWFVFAP
                     QCMSTIAVTRRETNGWKWPSFMLLYLFALAYIFAGITYWSAVALGL"
     gene            2211..2741
                     /gene="ssb"
                     /locus_tag="VFV30_07730"
     CDS             2211..2741
                     /gene="ssb"
                     /locus_tag="VFV30_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX4848017.1"
                     /translation="MAGSVNKVILIGNLGADPEVKSFQNGGRICNLRIATSENWKDKN
                     TGERKERTEWHTVVLNSDGLVGVAERYLRKGSKVYIEGSLRTRKWQDQSGNDRYSTEV
                     NVGMNGVLTMLDGAPGGGGAGGGGGGGYGGGQRSGGSDFGGGASGGQRSGGGGFGGGT
                     QGGGGFADDLDDDIPF"
     gene            2826..3233
                     /locus_tag="VFV30_07735"
     CDS             2826..3233
                     /locus_tag="VFV30_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848018.1"
                     /translation="MAGSRSYKSQMTILGTASLGAAALAGLVGSQVLNRQAGPADFWL
                     TYLPLLALCAFAFWAMTPWWRRLDDLQRQGQQSAWFWGGQIGGIVVLMALVAAAGRDS
                     DLARGGLYVVLGQAVGFAVAWLVWRWRSSGPAE"
     gene            3230..3427
                     /locus_tag="VFV30_07740"
     CDS             3230..3427
                     /locus_tag="VFV30_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4848019.1"
                     /translation="MRNRLRELRRERGLNQADLAEQLQVSRQTIIAIEADKYDPSLPM
                     AYRLAAFFDIPVEDLFFNPYR"
     gene            complement(3438..>3727)
                     /locus_tag="VFV30_07745"
     CDS             complement(3438..>3727)
                     /locus_tag="VFV30_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX4848020.1"
                     /translation="GEDLAVRIDEPLTLRFVPSGESPAGDEEIELAAEELDDIEMENG
                     RFDLGEAVAQTLALAIDPYLEGPNAEEFRRKAGILSEGEGGAFAALKNLLK"
BASE COUNT          569 a         1202 c         1282 g          674 t
ORIGIN      
        1 cggtaatggt tggtgcaagc caatcgcgga ggcgattggc gcatccagcg aatttgacct
       61 cgatcaatta ttgcgaatga cttgcaataa gcgccgggct gtggcagcgg cgggatcatg
      121 acactcgacc agatcccgat cgggagttcc gcgcgcattg ttgcggtcga ttggccggcc
      181 ctggtgccgg aagaggcgct gcgcctgcag gccctgggaa tcgatgcggg cgcgcgggtc
      241 gcggtcgatc atcgcggcat tttcggaacg cgcgatccga ttgccctggc catcggccgg
      301 atgacggtgg cgctgcgccg cgcccatgcc cgcgcgatcg aggtggaact gctgtgaccc
      361 tgcgcaccgc tgccctggtg ggcaacccca acgccggcaa gagcgccctg ttcaacgcgc
      421 tgaccggcgc gcgccagaag atcgcgaact atcccggcgt cacggtcgag cgcaaagccg
      481 ggcggctgac cctgccctcg ggcgaagcga tcgagctgac cgacctgccg gggtcctacg
      541 gcctcaatgc caccagcccg gacgaagagg tgacccgccg ggtgatcctg ggcgaactgc
      601 cgggcgaagc ggcgccgcaa gtgctggtgg tggtgctcga tgcctcgaac ctggaacagc
      661 atctggtctt tgcccaggaa gtgatcgcgc tggggcggcc gaccgtggtc gcgctcaaca
      721 tggtcgacct ggccgagcgc gacgggctgg tgatcgatcc ggccgcgctg gaagaggcgc
      781 tgggcgtgcc ggtgatcgcc accgtcgcgg tgcgccgccg cggcctgccc gaactggccg
      841 ccgcgatcgc cgccgcggaa gagcgcgcgc ccgaacacca ccgcccgcac accacgctga
      901 ccgaacggcg cgtgaccgcc caggcgattg ccgaggccgc gatcgtctcg gaaaccagcc
      961 gccaccgcct ccacgcccgg ctcgaccggc tgtttctcca cccctggctg ggcccgatga
     1021 tcctgttcgc catgctgttc gtggtgttcc aggcggtgtt cacctgggcg accccctttg
     1081 ccgacgcgct ggaaggcctg atcggcttgc tgagcgaaca ggcccgcgcc gtgttgcccg
     1141 aaggcctgcc gcgcgatctg ctgaccgacg ggatcctttc gggcgtcggc tcggtggtgg
     1201 tgttcctgcc gcagatcgtg atcctgttct tcttcatctt ggcgatggag gcttcgggct
     1261 atatggcccg ggccgcgttc ctgatggacc ggctgatggc cggtgtcggc ctgtccgggc
     1321 gcagcttcat tccgctgctg tccagctttg cctgcgcgat tcccgggatc atggcgaccc
     1381 gcagcatcac cgatccgcgc gaccggctga ccacgatcct ggtcgccccg ctgatgacct
     1441 gttcggcgcg gctgccggtc tatgcggtga tcatcgccgc cttcattccc aaccgttcgg
     1501 tgctggggcc ggtcggcctg caagggctgg tcctgctggg gctctatgtc ttcgggatcg
     1561 tcgcggcgat gctggtcgcg ctggtgctgc gccgctcggt caccaagggc gcggcctcgg
     1621 gcttcatcat ggaactgccc aagtaccaga tgccgcggct gaaggacctg ctgatcggcc
     1681 tgtggcagcg cgcctggatc ttcctgcgcc gcgccgggac gatcatcttc atggtcaccg
     1741 tggtgctgtg ggtgctgctc aacttcccgc gcgcgggcga agggcagaac caggtcgatg
     1801 cctcgattgc cggcaagatc gccaacggcc tggccgtggt ggtcgaaccg atcggcttca
     1861 accgcgatat cgccctgtcg ctgatcccgg ccatggccgc gcgcgaagtg gcggtcagct
     1921 cgctcgccac ttcctatgcg gtgggcgccg acgacgagga ggcccaggcc caggaactgg
     1981 gcgaacggct caaagccgac tggaccctgc cgaccgcgct ggccttcctg gcctggtttg
     2041 tcttcgcgcc ccagtgcatg agcacgatcg ccgtcacccg gcgtgaaaca aacgggtgga
     2101 aatggcccag cttcatgttg ctctatctgt tcgcgctggc ctacatcttc gcgggaatca
     2161 cctactggag cgccgtggcg ctcggattat agcgaagaaa gaaggacatc atggcaggca
     2221 gcgtcaacaa ggtcatcctc atcggtaacc tgggcgctga cccggaagtg aagtcgttcc
     2281 agaacggcgg gcggatctgc aacctgcgga tcgcgacctc ggaaaactgg aaggacaaga
     2341 acaccggtga gcgcaaggag cggaccgagt ggcacaccgt ggtgctcaat tccgacggtc
     2401 tggtcggcgt ggccgagcgc tatctgcgca agggcagcaa ggtctacatc gaaggcagcc
     2461 tgcgcacccg caagtggcag gaccagtcgg gcaatgaccg ctattcgacc gaagtgaacg
     2521 tcggcatgaa cggcgtgctg accatgctgg atggcgcgcc cggtggcggc ggtgcgggcg
     2581 gaggcggtgg cggcggctat ggcggcggcc agcgcagcgg tggcagcgat ttcggcggcg
     2641 gcgcttcggg tggccagcgt tcgggcggtg gcggcttcgg cggcggcacg cagggcggcg
     2701 gtgggttcgc tgacgacctg gacgacgaca tcccgttctg agcgccgacc ggatccaatg
     2761 taaaaaggta tttacattcc ctcgcctcga tgtaaaaaca tatttacatc gaggaggagg
     2821 atcgcatggc tggttcgcgt tcgtacaagt cgcaaatgac aatcctgggc accgcatcgc
     2881 tcggtgcagc ggctctggcc ggattggtcg gcagccaggt cctcaaccgg caagccggtc
     2941 cggcagactt ctggctgacc tatctgccct tgctggcgct gtgcgccttt gcgttttggg
     3001 ccatgactcc gtggtggcgc cggctggacg atctgcaacg ccagggccag cagtccgcgt
     3061 ggttctgggg cggccagatt ggcgggatcg tggtcctgat ggccctggtg gccgcggcag
     3121 gccgcgacag cgatctggcc cgcggcggcc tttatgtcgt gcttggccaa gcggtgggtt
     3181 tcgcggttgc ctggctggtc tggcgctggc gcagcagcgg gccggctgaa tgaggaaccg
     3241 gcttcgcgaa ttgcgccgcg aacgcggtct caaccaggcc gatctggccg agcagttgca
     3301 ggtttcccgc cagacgatca tcgcgatcga ggcggacaag tacgaccctt cgctgccgat
     3361 ggcctaccga ttggccgcct ttttcgacat tccggtcgaa gacctgttct tcaaccctta
     3421 ccgctgagtg gcgagggcta tttcagcagg ttcttcagcg cggcaaaggc gccgccttcg
     3481 ccctcgctga ggatgccggc cttgcggcgg aactcctcgg cgttggggcc ttccagatag
     3541 gggtcgatgg ccagggccag ggtctgggct acggcctcgc ccagatcgaa ccggccgttt
     3601 tccatctcga tgtcgtccag ctcttcggcg gcgagttcga tttcctcgtc gccggccggg
     3661 ctctcgcccg aggggacgaa gcgcagggtc agcggctcgt cgatccgcac cgccagatcc
     3721 tcgcccg
//
LOCUS       DASUDV010000352         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_188467,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000352 DASUDV010000000
VERSION     DASUDV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188467"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFV30_07750"
     CDS             complement(<1..220)
                     /locus_tag="VFV30_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX4848021.1"
                     /translation="MTQVERNAMGRRAVLAGAAASAVLTLPGCSSVGGFSMVEAIKRL
                     LTLSSQNAFAKLTAPGGFWDSSVARFEVP"
     gene            304..>2006
                     /gene="priA"
                     /locus_tag="VFV30_07755"
     CDS             304..>2006
                     /gene="priA"
                     /locus_tag="VFV30_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX4848022.1"
                     /translation="MNRVRLLVFNPVLSVLDYRLPTGMATEFGSLVLAPLGPRQVLGI
                     VWEPERLPANEVPEAKLRPILEVLPVPPLSEALRRLIEWCADYYVAPMNAVARMALGS
                     MAALRGGGTATEYRLSGIEPKRLTPQRAAAMDALHGEQASIRELAEFAGVSEGVLRGM
                     VSAGILEPVTVDLDRSYPRARPDHAQPDLSADQRAAADQFIAAVQARQFAPFLLDGVT
                     GSGKTECYFEAVAEALRLGRQVLVLLPEIALTQNFLRRFEARFGAAPVLWHSSLKASE
                     RRRAWRAIISGEAQVVVGARSALFMPYRDLGLIVVDEAHEVSFKQDDGVRYNARDVAV
                     IRARFEAVPVILASATPALESLQLAEAGVYRKIDLPDRFGGASLPEIEVIDLRTAMPE
                     RGHWIAPPLVEKLKERLARGEQSLLFLNRRGYAPLTLCRHCGYRFQCPNCSAWLVEHR
                     LSRRLACHHCGHETGLPEACPECHTGDCLVACGPGVERIADEVREILPDARVALVTSD
                     TLNSPEKIAGFVAAAEQKLIDVIIGTQLVTKGYHFPELTLVGVIDADLGLEGGDLRAG
                     ER"
BASE COUNT          311 a          666 c          683 g          346 t
ORIGIN      
        1 cgggcacttc gaagcgggca accgaactgt cccagaagcc gcccggcgcg gtcaatttgg
       61 caaaggcatt ctgcgacgag agcgtcagca gccgcttgat cgcctcgacc atcgaaaacc
      121 cgcccaccga actgcagccc ggcagggtca gcaccgcact ggccgcggca cctgccagca
      181 ccgcgcggcg acccattgcg ttgcgttcaa cctgcgtcat gatcccgcac tcccttgtct
      241 gtgcaccatc cgtgtggcgg ggctgtcgcc cgggcggctt tcgcgccata taggcaggcc
      301 ttgatgaacc gcgtccgact ccttgtcttc aaccccgtgc tttcggtgct ggactatcgc
      361 cttcctactg gaatggcgac agaattcggc tcgctggtgc tcgccccgct ggggccgcgg
      421 caggttctgg ggatcgtctg ggagcccgag cggctgcccg ccaacgaggt gcccgaggcc
      481 aagctgcgcc cgatccttga agtgctgccg gtaccgccac tgtccgaggc gctgcgccga
      541 ctgatcgaat ggtgcgcgga ctattacgtc gcgccaatga acgcggttgc tcggatggcg
      601 ctgggatcga tggcggcgct gcgcgggggc ggaacggcca ccgaataccg gctttcgggg
      661 atcgagccca agcggcttac cccgcaacgc gccgcagcga tggacgcgct gcacggcgaa
      721 caggccagca tccgcgagct ggccgaattc gccggcgttt ccgaaggcgt gctgcgcggc
      781 atggtcagcg ccgggatcct cgagccggtg acggtcgacc tggaccggtc ctatccgcgc
      841 gcccggcccg atcatgcgca gcccgatctt tccgccgatc agcgcgccgc cgccgatcag
      901 ttcatcgcag ccgtccaggc ccgtcagttc gccccgttcc tgctggatgg ggtcaccggc
      961 tcgggcaaga ccgagtgcta tttcgaagcc gtggcagagg cactgcggct gggccggcag
     1021 gtgctggtgc tgctgcccga aatcgcgctg acgcagaact ttctgcgccg gttcgaggca
     1081 cgtttcggcg cggcgccggt gctgtggcat tccagcctga aggcaagcga gcggcggcgc
     1141 gcctggcggg cgatcatatc gggtgaagcg caggtggtgg tcggcgcgcg ttcggcgctg
     1201 ttcatgcctt acagggacct tggcctgatc gtggttgacg aggcacacga ggtttcgttc
     1261 aagcaggacg acggggtgcg ttacaacgcg cgcgacgtgg cggtgatccg ggcccgcttc
     1321 gaagcggttc cggtgatcct ggccagtgct acgccggcgc tcgaatcgct tcagctggcc
     1381 gaggccgggg tctatcgcaa gatcgacctg cctgatcggt tcggcggggc cagcctgccc
     1441 gaaatcgagg tgatcgatct gcgcacggca atgcccgaac gcggccactg gatcgccccg
     1501 ccgctggtcg aaaaactgaa agagcggctg gcgcgcggcg aacagtcgct gctgttcctc
     1561 aaccgccgcg gctatgcccc gcttactttg tgccggcact gcggttatcg cttccagtgc
     1621 cccaactgtt cggcctggct ggtcgagcac cggctgtcgc ggcggctggc gtgccaccac
     1681 tgcggccatg aaaccggcct gcccgaagct tgccccgaat gccataccgg tgattgcctg
     1741 gtggcctgcg gcccgggggt tgaacggatc gccgacgaag tgcgcgaaat cctgcccgac
     1801 gcgcgggtcg cgctggtcac ttcggacacg ctgaacagtc cggagaagat tgccggcttc
     1861 gtcgccgcgg ccgaacagaa actgatcgac gtgatcatcg gcacccagct ggtgaccaag
     1921 ggctatcact ttcccgaact caccctggtg ggggtgatcg acgccgatct ggggctggaa
     1981 ggcggcgatc tgcgcgcggg cgagcg
//
LOCUS       DASUDV010000353         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_33919,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000353 DASUDV010000000
VERSION     DASUDV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33919"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VFV30_07760"
     CDS             complement(<1..1426)
                     /locus_tag="VFV30_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX4848023.1"
                     /translation="MKRCQGALGRALLLLAAAGLAVAPAHAAPRRDKAAEELKAQLLA
                     HITELSSDAYEGREPGTEGEAKTLRYLGAQWFQIGLQSGTNDPGNPWFAPVTIIAREP
                     DGSRAVFTRKGRRVFTPQGSVLALTSGRRSLVENAPVLFVGKGAEIPARAELAGRVAL
                     LLEGGNENSERQNALLAAGASAVLTVLDGDRSLENVAAHRIRKGYALADEVLGGDLEA
                     FITAEGIERALAGTGRTLASLRTTADAAGFTPIALDLAVTLEATTSETRIKTHNAIGK
                     LPGRRPELGAVVIMAHWDHFGRCVEPPAEDQICNGAIDNASGLAVMTEVARRLTKPGP
                     LDRDVYFVATTGEELGLLGAHAFAENPPLPLSRIVAAFNIDSVALAPAGRPFAVVGQG
                     MTKLDPQIAAAAKALKKKLAEGDGVNAFVRRQDGWALLQHDVPAVMVSSSWSDLALVE
                     RFMEGDYHRASDQLRPTIELGGAAE"
     gene            complement(1525..2280)
                     /locus_tag="VFV30_07765"
     CDS             complement(1525..2280)
                     /locus_tag="VFV30_07765"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX4848024.1"
                     /translation="MFLSGKRALVTGSTSGIGLAIARALHAEGAEVVLSGFGDADTIA
                     ALCDELRARHVPADLSRRDGVEALMAGSGPVDILVNNAGVQHVAPVEDFPVDKWDLII
                     ALNLTAAFDACRLAIPHMKAAGWGRIINTASAHSLTASPFKAAYVAAKHGLAGLTKTL
                     ALELATHNVTANCISPGYVWTPLVENQIPDTMAARGMSREEVIDQVLLIRQPTRKFVQ
                     PSDVGEMAVFLCRDAAANITGANISMDGGWTAE"
BASE COUNT          417 a          780 c          730 g          400 t
ORIGIN      
        1 cttcggccgc gccgcccagc tcgatcgtgg ggcgcagctg atcgctggcg cggtggtaat
       61 cgccttccat gaaccgttcg accagcgcca aatccgacca cgagcttgaa accatcaccg
      121 ccggcacatc gtgctgcaac agcgcccagc catcctggcg tcggacaaag gcgttgacgc
      181 catcgccttc ggccagcttc ttcttgagcg ccttggccgc cgccgcgatc tgcggatcga
      241 gcttggtcat gccctggcca accacggcaa agggccgccc ggcgggagcc agggcaaccg
      301 aatcgatgtt gaaggcggcg acgatccgcg aaagcggcag cggcggattt tcggcaaagg
      361 cgtgcgcccc cagaagcccc agttcctcac ccgtggtggc gacgaagtag acatcacgat
      421 cgagcggtcc tggtttggtc aggcgccgcg ccacctcggt catcaccgca aggccgctgg
      481 cgttatcgat tgcgccattg cagatctggt cttcggccgg cggctcgacg cagcgcccga
      541 agtgatccca gtgggccatg atcaccactg cccccagctc ggggcggcga ccaggcagct
      601 tgccgatcgc attgtgggtc ttgatccggg tttcgctggt agtcgcctcg agcgtcaccg
      661 caaggtcgag cgcaattggc gtgaaccctg ctgcatcggc agtggtcctg aggctggcca
      721 aagtgcggcc ggtacctgcc agggcgcgct cgatcccctc ggccgttatg aacgcctcga
      781 gatcaccgcc caggacctcg tcggccagcg catagccctt gcggatgcgg tgcgcggcca
      841 cattctcgag gctgcgatcg ccatcgagca cggtcagcac cgccgacgcg ccggcggcaa
      901 gcaacgcgtt ctggcgttcg gaattctcgt tgccgccttc gagcagcagg gcaacccgac
      961 ctgccagttc ggctcttgcc gggatctccg cacccttgcc aacgaacagc accggcgcat
     1021 tctcgacaag gctgcgtcgg cccgatgtca gcgcgagaac cgaaccttgc ggggtgaaga
     1081 cccgtctgcc tttgcgggta aatacagccc ggctgccatc gggctcgcgg gcgatgatcg
     1141 taaccggggc aaaccaggga ttgcccggat cgttggtgcc cgattgcagt ccgatctgga
     1201 accactgcgc ccccagatag cgcagcgtct tggcctcacc ctcggtcccg ggctcgcgcc
     1261 cttcataggc atcgctggag agttcggtga tgtgggctag cagctgggcc ttgagctctt
     1321 cggccgcctt gtcgcgccgg ggtgcggcgt gtgccggggc aacagccagg ccggccgcgg
     1381 caagcagcag caaggctcgt cccaacgccc cctggcaacg tttcatccca ccttgatgac
     1441 actgcgcccc aatcccgaca agcgggatcg aaaatcacac taaaaaagca agatcttgcg
     1501 tggcaggaat gtcaccaatc ctggtcactc cgcagtccat ccgccatcca tcgaaatatt
     1561 cgcgccggtg atattggctg cggcatcgcg gcacaggaac acggccattt cgccgacatc
     1621 ggatggctgg acaaacttgc gggtgggctg acggatcagc agcacctggt cgatcacttc
     1681 ctcgcggctc atcccgcggg ctgccatggt atcggggatc tggttctcga ccagcggggt
     1741 ccagacatag cccgggctga tgcaattggc ggtaacgttg tgggtggcca gttcaagcgc
     1801 cagggtcttg gtcagcccgg cgaggccatg cttggccgcg acataggcgg ccttgaaggg
     1861 cgatgcggta agcgaatggg cgcttgcggt gttgatgatc cgcccccacc cggccgcctt
     1921 catgtgcggg atcgccaggc ggcaggcatc gaaggcggcg gtcaggttga gcgcgatgat
     1981 cagatcccac ttgtcgaccg gaaagtcctc gaccggggcg acatgctgaa cgccggcgtt
     2041 gttgaccagg atatcgaccg gcccggaccc ggccataagc gcttccaccc cgtcacggcg
     2101 cgaaagatcg gcgggaacgt ggcgcgctcg caattcgtcg cacagcgcgg cgatcgtatc
     2161 ggcatcgcca aatcccgaaa gcaccacttc cgccccttcg gcgtgcagcg cccgcgcgat
     2221 cgcaaggccg atgcccgagg tcgatccggt gaccagcgcc cgctttcccg acaagaacat
     2281 tcccgactcc acctcgacaa ataaagcact atagtcagtg ccattgc
//
LOCUS       DASUDV010000354         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1601923,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000354 DASUDV010000000
VERSION     DASUDV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601923"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFV30_07770"
     CDS             complement(<1..329)
                     /locus_tag="VFV30_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848025.1"
                     /translation="MTARLRRDDPSNLNNGLAARRPRRHRLLGAGLVVLGGLGALAFY
                     PGGAEKAPQALLDAGVRKAFAMVPGGAVDCAPRPQALQAELEQIAASFDGKVGIAVTQ
                     AGCDWVVG"
     gene            complement(420..1559)
                     /locus_tag="VFV30_07775"
     CDS             complement(420..1559)
                     /locus_tag="VFV30_07775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4848026.1"
                     /translation="MNDDSSLEAAFAAASLPGMAALIVDRDGVRHAQALGLADVESGA
                     AMAIDTPCQIASMTKALTSLAAMQLVEAGRIALDDPVGPLLPELAQPRVLTGFDPQGK
                     PETRPAARAITLRHLLTHTAGCGYFFVNPEVLRYFGAVGMPAPGSRAALDVPLLFDPG
                     EGWAYSVATDWVGLAIEALTGGSLGDYLAEHLFEPLGMASTRFATELPGDAARIHTRL
                     AEGGFRAMPTFMGGGEYHNGGGGLISTASDYARFVRAMLRGGELDGRRVLSAAGVAEM
                     GRNQVGALRAGAMGSVMPGFALPFDPFPDQHSGWGLGFVINPEPGPNGRSAGSLAWAG
                     VANSYYWIDPAAGLGGVLMAQLSPFADPGALGLFGALERLAYRRD"
     gene            1602..>2436
                     /locus_tag="VFV30_07780"
     CDS             1602..>2436
                     /locus_tag="VFV30_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX4848027.1"
                     /translation="MTDRLTITLAQLNQSVGDIAGNAQAMLAARARARSAGADLIVFP
                     EMHLIGYPPEDLVLKPALIERAATQLEGLARATADGGPAMLVGSVFVVDGALHNGVAL
                     LDGGKVAAIRLKHELPNYGTFDEVRLFQAGPLPEPVEFRGVMIGLPICEDIWHPDVCR
                     HLAEFGAELFICINGSPYEIDKDTLRIDGVAKRRAVDTGLPLAYLNRVGGQDELVFDG
                     ASFVVNGDGSLAVQMKDWEESEVTTRWTRTAQGWRCDQGALAPLADHPEDIYCAMVLA
                     LR"
BASE COUNT          409 a          859 c          814 g          354 t
ORIGIN      
        1 ccgaccaccc agtcgcagcc ggcctgggtt accgcgatcc cgaccttgcc atcgaacgag
       61 gcggcgatct gttcgagttc ggcctgcagc gcctgcggcc ggggagcaca gtccaccgcg
      121 cctcccggca ccatggcaaa ggccttgcgc accccggcgt cgagcagcgc ctggggggcc
      181 ttttccgccc cgccgggata aaacgcaagc gcgcccagcc cgcccagcac caccaggccc
      241 gcgcccagca ggcgatgccg ccgtggccgc cgggcagcca ggccattgtt caggtttgac
      301 gggtcatcgc gtcgcaggcg tgcagtcatg tcgccaagag agataaccac tggatcgcgc
      361 cattccaacc actgtcgcgg caaaaaccgc cgatctcccc agccgcgacc ggccagggtt
      421 caatcgcgac gataggccag ccgttccagc gccccgaaca ggcccagcgc gccgggatcg
      481 gcgaagggcg aaagctgggc catcagcacc ccgccaagcc cggccgccgg atcgatccag
      541 taataggaat tggcaacgcc cgcccaggcc agactcccgg ccgaacggcc gttgggaccg
      601 ggttcggggt tgatcacgaa gcccagcccc cagccgctgt gctgatcggg gaacggatcg
      661 aacggcagcg cgaagccggg catcacgctg cccatcgccc cggcgcgcaa ggcgccaacc
      721 tggttgcggc ccatttccgc cacgcccgcg gccgacagca ctcggcgccc gtcaagctcg
      781 ccgccgcgca gcatggcccg cacgaaccgg gcatagtcgc tcgcggtgct gatcagcccg
      841 ccaccgccgt tgtggtattc cccgccgccc atgaaggtcg gcatggcccg aaacccgcct
      901 tcggccaagc gagtgtggat ccgcgccgca tcgccgggca attcggttgc gaaacgggtc
      961 gaggccatgc ccagcggctc gaacaggtgt tcggccaggt aatcgcccag gctgccaccg
     1021 gtcagcgcct cgatcgcgag accgacccag tcggtcgcca ccgaataggc ccagccctcg
     1081 cccggatcga acagcagcgg cacgtcgagc gccgcgcgcg atcccggcgc cggcatgccc
     1141 accgcgccga aataccggag cacttcggga ttgacgaaga aatagccgca gcccgcggtg
     1201 tgggtcagca ggtggcgcag ggttatcgcg cgcgccgcgg ggcgggtttc aggcttgcct
     1261 tgcggatcga acccggtgag cacgcgcggc tgggccagtt cgggcagcag cggcccgacc
     1321 ggatcgtcga gcgcgatccg tcccgcttcg accagctgca tcgccgcgag cgaggtcagc
     1381 gccttggtca tcgaggcgat ctggcacggg gtatcgatcg ccatggccgc gccgctttcg
     1441 acatcggcaa gccccagcgc ctgggcatgg cggaccccat cgcgatcgac gatcagggcc
     1501 gccatgcccg gcaggctggc ggcggcgaat gcggcttcaa ggctggaatc atcgttcatg
     1561 tggcattggc taatgccaag cttggcccta ggaaagcttt catgaccgac cggctcacga
     1621 ttaccctcgc ccagctcaac cagtccgtcg gcgatattgc cggcaatgcc caggccatgc
     1681 tggccgcgcg ggcgcgggcg cgcagcgccg gggccgatct gatcgtgttc cccgaaatgc
     1741 acctgatcgg ctatccgccc gaagatctgg tgctgaagcc cgcgctgatc gaacgtgccg
     1801 caacccagct cgaaggcctg gccagggcca ccgccgatgg ggggccggcg atgctggtgg
     1861 gttcggtctt cgtggtcgat ggcgcgctgc acaatggcgt ggcgctgctc gatggcggca
     1921 aggtcgcggc gatccgcctc aagcacgaac tgcccaatta tggcactttc gacgaggtcc
     1981 gcctgttcca ggccgggccg ctgcccgaac ctgtggagtt caggggggtg atgatcggcc
     2041 tgccgatctg cgaggatatc tggcaccccg atgtctgccg ccacctcgcc gaattcggcg
     2101 ccgaactgtt catctgcatc aacggcagcc cttacgagat cgacaaggat acgctgcgga
     2161 tcgatggcgt ggccaagcgc cgcgcggtcg ataccggtct gccgctggcc tacctcaacc
     2221 gggttggcgg gcaggacgag ctggtgttcg atggcgccag cttcgtggta aacggcgatg
     2281 gcagtctggc cgtgcagatg aaggactggg aggagagcga ggttaccacc cgctggaccc
     2341 gcaccgcgca gggctggcgc tgcgatcagg gcgcgctggc cccgctggcc gaccacccgg
     2401 aggacatcta ttgcgccatg gtcctggcgc tgcgcg
//
LOCUS       DASUDV010000355         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_851195,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000355 DASUDV010000000
VERSION     DASUDV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851195"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFV30_07785"
     CDS             complement(<1..255)
                     /locus_tag="VFV30_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX4848028.1"
                     /translation="MSAPQLDDLDRQLIEVLARDARVSNRKIAGDLGVTEGTVRGRIK
                     RLQQDGLISFTAITSFGLADSARMAFIGVQAEVSEVREVAR"
     gene            complement(252..731)
                     /locus_tag="VFV30_07790"
     CDS             complement(252..731)
                     /locus_tag="VFV30_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX4848029.1"
                     /translation="MSEAFSIDDVDRAIVEQLRVNGRATNQQIANTLGLTAATVSARI
                     RRMEEADKLRVVAVSDFSAHGYNVLMEVAIEVDGRPASEVASELAEFPEVFAAHLVTG
                     RYDIDMLVVLRDIGDLPHLLLDKFSKVKGIRSMVPAIAVDVIKYKFDVAPIDAREDA"
     gene            complement(803..1291)
                     /locus_tag="VFV30_07795"
     CDS             complement(803..1291)
                     /locus_tag="VFV30_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4848030.1"
                     /translation="MNAEAGFAAPQELHDLAFRYGLAVDSRDPAMLAAVFTREGTLRG
                     LGEDKARYTGLVGWERMIAEVSASFGQTLHNVFNQTFDRAGDGTVTGLTTGIASHLLP
                     VDEGTAAVPLLDFAMRYHNRYAQEDGRWKFADRQLEVVWVERRQVARFDPAMLGRELK
                     GF"
     gene            complement(1288..2571)
                     /locus_tag="VFV30_07800"
     CDS             complement(1288..2571)
                     /locus_tag="VFV30_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848031.1"
                     /translation="MHPSAAPRSNPWVVLATLLVIYIFNFADRYLLTGLIGPIKQEFE
                     IGDGMIGLLMGPAFVVLYVVMGVPFARLADRGSRIRIIAAGCVLWSLATAATGLATGP
                     VSLGLARIGVGVGEAAFVAPAYSLLTDYFKPERRGLAFAILGLATYFGQIAGQAGGPA
                     LAAEIGWRTTFFAVGLPGVALGVIALLLVREPPRVLAAGAQAVAAIPFGQLIGLLLRS
                     PGYVMMALGFGLGTLSGVTFGFWGPELFTRSFALDPVTVKTAFALNFGLAGLCGMLAF
                     GALSDKLSQRGRVWPVRLSGLALLCATALVLVTVWAPSFELAMLVAIPCGLLGGGWSV
                     GIYAGLQHMLPASYRASATAVFIAFITLLGYFVGPWATGAISQALGDDAASLQVAISV
                     VVPAGFLAALFALMAAGRVEADRELLARLEAERAA"
     gene            complement(2571..3329)
                     /locus_tag="VFV30_07805"
     CDS             complement(2571..3329)
                     /locus_tag="VFV30_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4848032.1"
                     /translation="MRGLKGKVGVVAGGGRGIGAATARRLAEEGAAVVIGDITEEWAR
                     ETAEAIRAAGGRAIGVYLDGTKAASQGAIVQAALDEFGGLDFYHSNLAGGTEGDIDAL
                     NCPEEVLDRSFAINTKSHMFATQAALPVMLERGGGAMIYTSSGAAIGGAPFQVAYPMT
                     KNAIHALVRHVAAKYGKQGIRSNGICPGVVLTEAVKQHLTDEYVAAALKTVPHTRLGD
                     PDDIAGAVAFLASDDGAWVNGQVWHVNGGMIKRD"
BASE COUNT          603 a         1214 c         1083 g          511 t
ORIGIN      
        1 gcgggccact tcgcgcactt cgctgacttc ggcctggacg ccgatgaagg ccatccgcgc
       61 ggaatcggcc aggccgaagc tggtgatcgc ggtgaacgag atcagcccgt cctgctgcag
      121 ccgcttgatc cgcccgcgca cggtgccttc ggtgacgcca aggtcgccgg caatcttgcg
      181 gttggagacg cgggcatcgc gggccaggac ctcgatcagc tggcggtcga gatcatcgag
      241 ttgcggcgcg ctcatgcgtc ctcccgcgcg tcgatcgggg cgacgtcgaa cttgtacttg
      301 atgacgtcga cggcgatcgc cggaaccatc gagcggatgc ccttcacctt ggagaacttg
      361 tcgagcagca ggtgcggcag atcgccgatg tcgcgcagca ccaccagcat gtcgatgtca
      421 tagcgcccgg tcaccaggtg cgcggcgaag acttcgggaa actcggccag ctcgctcgcc
      481 acttcggagg ccgggcggcc atcgacctcg atcgccactt ccatcagcac gttgtagcca
      541 tgggccgaaa agtcggacac cgcgacaacc cgcagcttgt cggcttcctc catccgccgg
      601 atccgcgcgg ataccgtcgc cgccgtcagc cccagggtgt tggcgatctg ctggttggtg
      661 gcgcggccat tgacgcgcaa ttgctcgaca atcgcgcgat cgacgtcatc gatcgagaag
      721 gcttcactca ttgcccggtc aaacccccaa attgccgttc cgtcaacact tcggcccccg
      781 aaatgcggca caaatccacg attcaaaagc ccttgagctc gcgccccagc atggccggat
      841 cgaaccgcgc cacctggcgc cgttccaccc agaccacttc cagctggcgg tcggcgaatt
      901 tccagcgccc gtcctcctgc gcatagcggt tgtgatagcg catggcgaaa tccagcagcg
      961 gcaccgccgc ggtgccctcg tccaccggca gcaggtggct ggcgatcccg gtggtcagcc
     1021 cggtcaccgt gccatcgccg gcgcggtcga aggtctggtt gaagacattg tgcagggtct
     1081 ggccgaagct ggccgaaacc tcggcgatca tccgttccca tccgactagg ccggtatagc
     1141 gggccttgtc ctcacccagg ccgcgcagcg tgccctcgcg ggtgaagacc gccgccagca
     1201 tggccggatc gcggctgtcg accgccaggc catagcgaaa cgccagatcg tgcagctcct
     1261 gcggggccgc aaaaccggct tcggcgttca cgccgcgcgc tccgcttcca gccgcgccag
     1321 cagttcccgg tccgcttcaa cccgcccggc cgccatcagc gcgaacagcg cggcaagaaa
     1381 gccggccggc accaccacgc tgatcgccac ttgcagcgat gccgcatcgt cgcccagcgc
     1441 ctggctgatc gcgccggtcg cccagggacc gacgaaatag cccagcaggg tgatgaaggc
     1501 gatgaacacc gcagtcgcgc tggcgcggta gctggccggc agcatgtgct gcagcccggc
     1561 atagatcccg accgaccagc ccccgccaag gagaccgcag gggatcgcca ccagcatcgc
     1621 gagttcgaaa ctgggcgccc agaccgtgac cagtaccagg gccgtggcgc agagcagggc
     1681 caggcccgag agccgcaccg gccagacccg cccgcgctgc gacagcttgt ccgacagcgc
     1741 gccgaacgcc agcatcccgc acaggccggc aaggccgaag ttgagcgcga aggcggtctt
     1801 gaccgtcacc ggatcgagag cgaagctgcg ggtgaacagt tccggtcccc agaaaccgaa
     1861 cgtcacgccg gacagcgtgc ccaggccaaa gcccagcgcc atcatcacat agccggggct
     1921 gcgcagcagc aggccgatca attgcccgaa cggaatcgcc gccaccgcct gcgccccggc
     1981 ggccaggacc cggggcggct cgcgcaccag cagcagggcg atgacgccca gcgccacgcc
     2041 cggcaggccc accgcgaaga aggtggtccg ccagccgatc tctgccgcca gtgccggtcc
     2101 gccggcctgc ccggcgatct gtccgaaata ggtcgccagg cccaggatcg cgaaggccag
     2161 gccgcgccgc tccggcttga aatagtcggt cagcagcgaa taggccgggg cgacgaaggc
     2221 cgcctcgccc acccccacgc cgattcgcgc gagcccgagc gaaaccggcc cggtggcaag
     2281 gccggttgcc gccgtggcaa ggctccacag cacgcagccc gccgcgatga tccggatccg
     2341 cgatccgcgg tcggccagcc gtgcgaaagg cacccccatc accacataga gcacgacgaa
     2401 ggccggaccc atcagcaggc cgatcatccc gtcgccgatc tcgaattcct gcttgatcgg
     2461 gccgatcagc ccggtcagca gatagcggtc ggcaaagttg aagatgtaga tcaccagcag
     2521 ggtggcaagc accacccagg ggttcgagcg gggggcggcg gacgggtgca tcagtcgcgc
     2581 ttgatcatgc cgccgttgac gtgccagacc tgcccgttga cccaggcccc gtcatccgag
     2641 gcgaggaagg ccacggcacc ggcgatgtcg tccggatcgc ccaggcgggt gtgcggcacg
     2701 gtcttgagcg cggctgcgac gtattcgtcg gtcaggtgct gcttgaccgc ttcggtcagg
     2761 accacgccgg ggcagatccc gttggaacgg atgccctgct tgccgtactt ggcggccacg
     2821 tggcggacca gcgcgtggat cgcgttcttg gtcatcgggt aggccacctg gaacggagcc
     2881 ccgccgatcg ccgcgccgga cgaggtgtag atcatcgccc cgccgccgcg ctccagcatc
     2941 accggcagcg cggcttgcgt ggcgaacatg tggctcttgg tgttgatcgc gaaggaacgg
     3001 tcgagcactt cctccgggca gttgagcgcg tcgatatcgc cctcggtccc gcctgcgaga
     3061 tttgagtggt agaaatccag cccgccgaat tcatcgagcg cggcctggac gatcgcgcct
     3121 tgcgaggcgg ccttggtgcc atcgagatag accccgatcg cccgcccgcc ggcggcgcgg
     3181 atcgcctcgg cggtttccct ggcccattcc tcggtgatgt cgccgatcac cacggcggca
     3241 ccttcttcgg ccagccgccg cgcggtggcc gcaccgattc cgcgcccgcc cccggcaacg
     3301 acaccgacct tgcccttcaa accacgcatg acactctcca tgactttgcg tttcaataat
     3361 ggctatttca tacgcaaact gctgtcaaaa ccaaagttca atttactttt t
//
LOCUS       DASUDV010000356         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1536001,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000356 DASUDV010000000
VERSION     DASUDV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536001"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFV30_07810"
     CDS             <1..511
                     /locus_tag="VFV30_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4848033.1"
                     /translation="RLGAAPGADGSADENGRLRTIATPVQPEWIDYNDHLTEYCYLKL
                     FGDATDVVLARIGAGPDYVASGHSWYTVETHIRHLGQARLGERIEVRSRVLAADSKRL
                     HLFHEMTSLAQGAVIATAEHMLVHVDAKAEKSAPAPQEMLDKAAALVAGQAHLPAPEG
                     QGRQIRMPG"
     gene            548..1957
                     /locus_tag="VFV30_07815"
     CDS             548..1957
                     /locus_tag="VFV30_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX4848034.1"
                     /translation="MALVESRSIDYVPLAERHGKVWHLWPIWFTGDAHMATLAVGAIS
                     VAAGLSLFWSAIAVLGGCALGTVLMAFHCTQGPHLGLPQMIQSRPQFGYLGALLVWVV
                     ALFTYIGYNALNQTLAADTLKELAGLPHGPTVVGFSIAAIAVAVIGYDLIHRAQRYLA
                     YAMLTVLAIYTVGAFRIVDFGTLLAPGEFKPTPFLVQFFAAAAYQLSWSIYVSDYSRY
                     LPKEVGIRDAFFWTFWGAFIGGAWMMLVGTFAAAAFASPDGIVAALRDSSDAIYPGFG
                     VVMLVTSFLGLITITSLNFYGASLTLLSVVDSLTKLKLGVKSRIASLFAVFAVALSLS
                     LMVSGDVLHGFEAFMAVLAYLFTPWTAINLVDFFFVRKGHYSIRAMFMADGLYGKWNW
                     RGLVSYFVGFAAMIPFFKTGIFVGPAAQALGNADISMLIGLPVSAAIYLYLCRDLDLK
                     SELARIAELDRNLETEPVR"
     gene            1954..3000
                     /locus_tag="VFV30_07820"
     CDS             1954..3000
                     /locus_tag="VFV30_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848035.1"
                     /translation="MKRAIALTVLAALTAPAQAKPTPANPIVFPSQFDSIGTPVQSLT
                     TKEGRKVHFTDTGEKGWKPVLFIGGVGTSGRAPELVAFLDTMRKRLKLRIISVERNGL
                     GDTAFDPAWTYADYNSEVRQVLGQLGIGQFALVAISGGGYYAGHIVSAMPERVASWHM
                     LAAVASAGGNVERCKADEAALAAQLGPQITAPRIWWDMPDDGVASKVRGFDDRTADEG
                     ARSAFMGGQMGDARGVAREYKRVCDPAADVSAFKGPAYFYYGEADKAVPPEQGRYWAS
                     QVKGKVTFRSYPGEGHDVQYRHWDQVMLDIAGYGSSTLVCSAGTARLVPGLITPAKLT
                     KGQTLGVCAWQTGG"
     gene            complement(3001..>3290)
                     /locus_tag="VFV30_07825"
     CDS             complement(3001..>3290)
                     /locus_tag="VFV30_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX4848036.1"
                     /translation="MDHWDTVLPGRVLRVNNEDVIEDLEGQVRRMLDFCGLPFEQSCI
                     EFHRNERAVRTPSSEQVRRPVNRDGTEVWKNYEPWLDPLKDALAGRSAPAA"
BASE COUNT          510 a         1091 c         1090 g          599 t
ORIGIN      
        1 gcggctgggc gcagcgccgg gcgccgatgg cagcgcggac gaaaacggcc gcctgcggac
       61 catcgccacc ccggtccagc cggagtggat cgactacaac gatcacctga ccgaatactg
      121 ctatctgaag ctgttcggcg atgcgaccga cgtggtgctg gcgcggatcg gcgccgggcc
      181 ggactatgtc gccagcggcc attcctggta cacggtggaa acccacatcc gccacctggg
      241 ccaggcgcgc ctgggcgaac ggatcgaggt gcgcagccgg gtgctggcgg ccgacagcaa
      301 acgcttgcat ctgttccacg aaatgaccag ccttgcgcaa ggtgcggtga tcgccacggc
      361 cgagcacatg ctggtccatg tcgatgccaa ggccgagaaa tcggcaccgg caccgcagga
      421 gatgctggac aaggctgcgg cccttgtcgc cgggcaggcc catctgcccg cgccagaggg
      481 tcagggccgg caaatcagga tgcccggata acgataacag ggcacgcttt ttgggggagt
      541 tgtttgcatg gccttggttg aatcccgctc gatcgactac gtccccttgg ccgaacggca
      601 cggcaaggtc tggcacctct ggccgatctg gttcaccggc gatgcgcaca tggccacgct
      661 ggcggtcggc gcgatcagcg ttgccgccgg gctgagcctg ttctggtccg ccatcgcggt
      721 gctgggcggc tgcgcgctgg gcacggtgct gatggctttc cactgcaccc aggggccgca
      781 cctgggcctg ccgcagatga tccagtcccg cccgcagttc ggctatctgg gcgcgctgct
      841 ggtctgggtc gtcgcgctgt tcacctacat cggctacaat gcgctcaacc agacgctggc
      901 ggccgatacg ctgaaggaac tggccggcct gccccacggc ccgaccgtgg tcggcttttc
      961 gattgcggcg attgcggtgg cggtgatcgg ttatgacctg atccaccggg cccagcgcta
     1021 tctggcctat gccatgctga cggtgctggc gatctacacg gtcggggcct tccggattgt
     1081 cgatttcggg acgctgctgg ccccgggcga gttcaagccg accccgttcc tggtccagtt
     1141 cttcgccgcc gccgcctatc agctcagctg gtcgatctac gtgtcggact actcgcgcta
     1201 cctgcccaag gaggtgggga tccgcgatgc cttcttctgg accttctggg gcgccttcat
     1261 cggcggggcc tggatgatgc tggtcgggac tttcgccgcc gccgccttcg ccagccccga
     1321 cgggatcgtt gccgcgctgc gcgacagttc cgatgcgatc tatcccggct ttggcgtggt
     1381 catgctggtc accagcttcc ttggcctgat cacgatcact tcgctcaatt tctacggcgc
     1441 ctcgctgacc ctgctcagcg tggtcgacag cctgaccaag ctgaagctgg gggtgaaatc
     1501 gcggatcgcc tcgctgttcg cggtcttcgc cgtggcgctc tcgctctcgc tgatggtcag
     1561 cggcgatgtg ctgcacgggt tcgaggcctt catggccgtg ctggcctatc tcttcacgcc
     1621 gtggaccgcg atcaacctgg tggacttctt cttcgtccgc aaaggccact attcgatccg
     1681 ggcgatgttc atggccgacg ggctgtacgg caagtggaac tggcgcgggc tggtgtccta
     1741 tttcgtcggg ttcgccgcga tgatcccgtt cttcaagacc gggatcttcg ttggcccagc
     1801 ggcccaggcg ctgggcaatg ccgacatctc gatgctgatc ggcctgccgg tttcggccgc
     1861 gatctacctc tacctgtgcc gcgatctgga tctgaagagc gagctggccc ggatcgcgga
     1921 actggatcgc aacctggaaa cggagccggt gcgatgaagc gcgcaatcgc cctcactgtc
     1981 ctggcggcgc tgacggcacc ggcccaggcc aagcccacgc cggccaatcc gatcgtgttc
     2041 cccagccagt tcgattccat cggcacaccg gtgcagagcc tgaccaccaa ggaaggccgc
     2101 aaggtccatt tcaccgacac cggggaaaag ggctggaagc cggtgctgtt catcggcggg
     2161 gtcggcacca gcggccgcgc gccagagctg gtcgccttcc tcgataccat gcgcaagcgg
     2221 ctgaagctgc ggatcatctc ggtcgagcgc aacggcctgg gcgatacggc cttcgatccg
     2281 gcctggacct atgccgatta caacagcgag gtccgccagg ttctgggaca gctcgggatc
     2341 gggcagttcg cgctcgtggc gatttcgggc ggcggctact acgccgggca catcgtctcg
     2401 gccatgccgg agcgggtcgc atcgtggcac atgctggccg cggtcgcatc ggcagggggc
     2461 aatgtggaac gctgcaaggc cgacgaggcc gcgctggccg cccagcttgg cccgcagatc
     2521 accgcaccgc gcatctggtg ggacatgccc gatgacggcg tcgcttcgaa agttcgcggg
     2581 ttcgatgatc gcactgccga tgaaggtgcg cgctctgcct tcatgggcgg gcagatgggc
     2641 gatgcgcgcg gcgtggcccg cgaatataag cgggtctgcg atcccgcggc cgatgtctcg
     2701 gccttcaagg gcccggccta tttctattac ggcgaggccg acaaggccgt cccgcccgaa
     2761 cagggccgct attgggccag ccaggtcaag ggcaaggtca ccttccgctc ctaccccggc
     2821 gaaggccacg atgtgcagta ccgccactgg gatcaggtga tgctcgatat cgccggctac
     2881 ggcagcagca cgctggtttg cagcgccggc acggcgcggc tggtgccggg gctgatcacg
     2941 ccggccaagc tgaccaaggg ccagaccctg ggcgtctgcg cctggcagac cggcggctga
     3001 tcaggcggcc ggcgcgctcc gcccggccag cgcatccttc agcggatcca gccacggctc
     3061 gtaattcttc cacacctcgg tcccgtcgcg gttgaccggg cggcgcacct gctcggaact
     3121 gggcgtgcgg acggcgcgct cgttgcggtg gaattcaatg cacgattgct cgaacggcag
     3181 gccgcagaaa tcgagcatcc ggcggacctg cccttccaga tcctcgatca catcctcgtt
     3241 gttcacgcgc agcacgcggc ccggcaacac cgtgtcccaa tggtccatca
//
LOCUS       DASUDV010000357         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1381345,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000357 DASUDV010000000
VERSION     DASUDV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381345"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VFV30_07830"
     CDS             complement(<1..176)
                     /locus_tag="VFV30_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX4848037.1"
                     /translation="MPIEIKMPALSPTMEEGTLAKWLVKEGDTVSSGDIMAEIETDKA
                     TMEFEAVDEGTIVS"
     gene            302..754
                     /locus_tag="VFV30_07835"
     CDS             302..754
                     /locus_tag="VFV30_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4848038.1"
                     /translation="MRVYLVIMDETEEASTALRFAASRAARTGGQVHLLALVPRQQFV
                     AFGGIQATIEEEARARAEALVTSAAGSLLSEGGLAPTIAVRQGEGVAVIRGYLAEHPE
                     VSALVLGAAASGGPGPLVSHFAGVSGQLPCPVYIIPGGMSEADLERLS"
     gene            complement(756..3008)
                     /locus_tag="VFV30_07840"
     CDS             complement(756..3008)
                     /locus_tag="VFV30_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEX4848039.1"
                     /translation="MPRTPKPQPGLPSRQQILDFIAASAEPAGKREIARAFGLKGQEK
                     IQLKALLKDMADEGLIDGNRTAFHRMGGVAKVTVLRVIDTDDGEAVAVPDSWSPDDAT
                     PPPRLRLRESGKGSALRSGDRVLARTEETATGWIAHPMKKLPAAADQVLGVVEIDKSG
                     KGWLAPVDKRVRNSSPIADLGGAEAGQLVLAEPAGRSPRSGVKVTAVLGDPLAPKAFS
                     LIAIHKHGIPFAFSPETLAEGQLAAGLPLSEERREDLRHLPIVAIDPSDARDHDDAIW
                     AEPDGEGGFRALVAIADVSFYVRPGSDLDREARRRGNSVYFPDRVVPMLPEVLSADVC
                     SLRAGEDRAAMACHLVIGPDGKVTSWRFTRAIVRIQEVIAYEEAQRRIDQGEAATNLT
                     HLWEAWRALEAARKDRDPLDLDLPERRVVLDEHGKIREIALRERLDAHRVVEDFMIAA
                     NVAAAKALESKVSPVVYRIHEPPNREKLVALKDYLATFERKLSLGQVITPGLFNRMLK
                     DIADESEKALIMEAVLRSQTQAYYGPRNAGHFGLALGSYAHFTSPIRRYADLLVHRAL
                     VDGFGLEQPAPKGDLPPTSGLSDRDRADLSRVSEAISAAERRAMEAERETIDRYVAAW
                     LSARVGEVFPCRVTGVQRFGLFATIVGLGGDGLLPVSVLGDERFFHDEKAQALVGESS
                     GERYTLGMILRLRLAEANPLTGALKFEPEDSAGRIEPRGRPQPLKKKGKHFAGPRGRP
                     GNIRHQGKRR"
     gene            complement(3063..3512)
                     /locus_tag="VFV30_07845"
     CDS             complement(3063..3512)
                     /locus_tag="VFV30_07845"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEX4848040.1"
                     /translation="MRNALAFAFAFVASAAAAQADEWRLVAENEGRPGIAMNIDQASV
                     VRTGHTVTGWVMTVREYDPKAADWTKSLIFRQIDCDRNIAQMMQSKFYGPKGNLLEDT
                     KVPANWQNIVDGSMLEGVAFVMCGKDGYASGVVPDPMADARARFAKH"
     gene            complement(3596..>4148)
                     /locus_tag="VFV30_07850"
     CDS             complement(3596..>4148)
                     /locus_tag="VFV30_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4848041.1"
                     /translation="LAAQTALGEPVRVLLDKRAGKATQKRWGWVKKGVPLILEIGGRD
                     AASGQVSAVRRDRLYREDGKVAFVGQGKEDFLAAAAAELEDIQRSLHAEATARRDSHI
                     QRGISDLAGIEAFFAENQKYPGWVEVCWSRPTGAALDGVVAKLKALKLTIRNTPMDGA
                     PVSGTCPFTGEPAVERIYVARAY"
BASE COUNT          632 a         1402 c         1392 g          722 t
ORIGIN      
        1 atcgagacga tcgtgccttc gtcgaccgct tcgaattcca tcgtcgcctt gtcggtttcg
       61 atctcggcca tgatgtcgcc ggaagagacg gtgtcgcctt ccttgaccag ccacttggca
      121 agcgtgcctt cttccatggt cggcgaaagc gcgggcatct tgatttcgat gggcatggtg
      181 tgccttcagg tcctcgctgg ggagtgcgtg tcgctacgtc tctggccaga aattcgcgaa
      241 acggcaagac gcttgcgccg aatacgattg tatcggatat cccgcccgtg ggggatcgaa
      301 gatgcgcgtt tacctggtga tcatggacga gacggaggaa gccagcaccg cgctgcgctt
      361 tgccgcaagc cgtgccgccc gcaccggcgg gcaggttcat ctgctggccc tggtcccgcg
      421 ccagcaattc gtggcctttg gcggaatcca ggcaaccatc gaggaagaag cccgcgcccg
      481 ggccgaggcc ctggtcactt cggccgccgg cagcctgctg agcgagggcg gactggcgcc
      541 gaccatcgcg gtgcggcagg gcgaaggcgt ggcggttatc cgcggctatc tggccgaaca
      601 cccggaagtg tccgccctgg tcctgggtgc ggcggcaagc ggcggcccgg ggccgctggt
      661 ctcgcacttt gccggggttt cggggcaact gccctgcccg gtctacatca tccccggcgg
      721 gatgagcgag gccgacctgg agcggctgag ctgacctagc ggcgcttgcc ctggtggcgg
      781 atgttgccgg ggcgcccgcg ggggccggcg aagtgcttgc ccttcttctt gagcggttgc
      841 ggccgcccgc gcggctcgat ccggccggcg ctgtcctccg gctcgaactt caacgcgccg
      901 gtcagcggat tggcctcggc caggcgcagg cgcaggatca tgccgagggt gtagcgttcg
      961 ccgctgctct ccccgaccaa cgcctgggcc ttctcgtcgt ggaagaagcg ttcgtcgccc
     1021 agcaccgaaa ccggcagcag cccgtctccg cccagcccga caatggtcgc gaacaggccg
     1081 aagcgctgga ccccggtgac ccggcacggg aaaacttccc ccacccgggc cgagagccag
     1141 gccgccacat agcggtcaat cgtctcgcgc tcggcctcca tcgcccggcg ctcggccgcg
     1201 ctgatcgctt cggacacgcg cgagaggtcg gcccggtcgc ggtcggacag gccagaggtc
     1261 ggcggcaggt cccccttcgg cgcgggctgt tccagaccga agccgtcgac cagcgcgcgg
     1321 tgcaccagaa ggtcggcata gcggcggatc ggcgaggtga aatgggcata ggaccccagc
     1381 gccaggccga aatggccggc gttgcggggg ccgtaatagg cctgggtctg gctgcgcagg
     1441 acagcctcca ttatcagtgc cttttcagac tcatccgcaa tatccttcag catccggttg
     1501 aacagtcccg gagtgatcac ctggcccagc gagagcttgc gttcgaaagt ggcgaggtag
     1561 tccttcagcg ccaccagctt ttcccggttg ggcggctcgt ggatccggta gaccaccgga
     1621 gaaaccttgg attccagcgc cttggccgcg gcaacgttgg ccgcgatcat gaaatcctcc
     1681 accacccggt gagcatcaag ccgctcgcgc agggcgattt cccggatctt gccgtgctcg
     1741 tccagcacca cccgccgctc cggcaggtcg agatcgagcg gatcgcggtc cttgcgcgcc
     1801 gcttccagcg cccgccacgc ctcccacagg tgggtcagat tggtagcggc ctcgccctga
     1861 tcgatccggc gctgcgcctc ttcataggcg ataacctcct gaattcgcac gattgcccgg
     1921 gtgaaacgcc atgatgtcac ttttccgtcc gggccgatca ccaggtggca ggccatggcc
     1981 gcgcggtctt ccccggcgcg cagggagcag acatcggcgc tcagcacttc gggcagcatc
     2041 ggcaccaccc ggtccgggaa atagaccgag ttgccgcgcc gccgggcctc acggtcgaga
     2101 tcgctgccgg ggcgcacgta gaagctgaca tcggcaatcg cgaccagggc gcggaaaccg
     2161 ccttcgccgt ccggttcggc ccagatcgca tcgtcgtggt ctcgcgcgtc gctggggtcg
     2221 atcgcgacga tcggcaaatg ccgcagatcc tcgcgccgtt cctcgctgag cggcaggccc
     2281 gcagcgagct gcccttcggc cagcgtttcg gggctgaagg caaagggaat gccgtgcttg
     2341 tggatcgcga tcaggctgaa ggccttgggg gccagcggat cgccgaggac ggcggtgacc
     2401 ttcactccgg aacgcgggct gcgtccggcc ggttcggcca gcaccagctg tcccgcctcg
     2461 gccccgccca gatcggcaat cgggctggaa ttgcggaccc gcttgtcgac cggggccagc
     2521 cagcccttcc cgctcttgtc gatttccacc acgcccagca cctgatcggc cgcggcgggc
     2581 agtttcttca tcggatgggc gatccagccg gtcgcggtct cctcggtccg ggccagcacc
     2641 cggtcgccgc tgcgcagggc ggaacccttt ccggattcgc gcaggcgcag gcgcggcggc
     2701 ggggtagcgt cgtctggaga ccagctgtcg ggtacggcaa ccgcctcgcc gtcgtcagta
     2761 tcgatcacgc gcagcacggt gaccttggcg accccgccca tgcggtggaa cgcggtgcgg
     2821 ttcccgtcga tcaggccctc gtccgccatg tccttgagca gggccttgag ctggatcttc
     2881 tcctgcccct tcaggccaaa ggcgcgcgcg atctcacgct tgccggccgg ttcggcgctg
     2941 gcggcgatga aatcgaggat ttgctggcgg cttggcaggc cgggttgcgg cttaggggta
     3001 cggggcatgg cgccttgtcc acgcggcgcg cggacaaggc aagctgcaaa agccgcttat
     3061 gatcagtgct tggcgaagcg ggcccgggcg tcggccatcg gatcgggaac cacgccgctg
     3121 gcatagccat ccttgccgca catcacgaag gccacgcctt caagcatcga cccgtcgacg
     3181 atgttctgcc agttggccgg caccttggtg tcttccagca ggttgccctt cgggccgtag
     3241 aacttggact gcatcatctg ggcgatgttc cgatcgcagt cgatctggcg gaagatcagc
     3301 gacttggtcc agtcggccgc cttgggatcg tattcgcgca cggtcatcac ccagccggtg
     3361 acggtgtgac ctgtgcggac cacgctggcc tggtcgatat tcatggcgat gccgggccgg
     3421 ccttcgttct ctgccaccag gcgccattcg tcggcctggg ccgccgcagc gctggcgaca
     3481 aaggcaaaag cgaaagcaag tgcgttacgc atagtcattt tcccctcaag gcgacgaatc
     3541 gcgtgatggg tgaggctatg ccggatcgcg cgccaagtca ccttggcgaa atgcgtcagt
     3601 atgcgcgggc gacgtagatc cgctcaaccg ccggttcgcc ggtgaagggg caggttccgc
     3661 tgacgggagc gccatccatc ggggtgttgc ggatcgtcag cttgagcgcc ttgagcttgg
     3721 ccacaactcc gtccagcgcg gccccggtcg gccgcgacca gcagacttcg acccagcccg
     3781 gatacttttg gttctcggca aagaaggctt cgatcccggc cagatcgctg atcccgcgct
     3841 ggatatgcga atcgcgccgc gcggtggctt cggcgtggag cgaacgctgg atgtcctcca
     3901 gctccgcagc ggcggcggcg aggaagtcct ccttgccctg gccgacgaag gcgaccttgc
     3961 cgtcctcgcg gtagagtcgg tcgcggcgca cggcggaaac ctgcccgctg gcggcatcgc
     4021 gcccgccgat ttccaggatc aggggcacgc ccttcttgac ccagccccaa cgcttctgcg
     4081 tggccttgcc cgcccgcttg tcgagcagca cgcggaccgg ctcgcccagc gcggtctgag
     4141 cggccagc
//
LOCUS       DASUDV010000358         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_254635,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000358 DASUDV010000000
VERSION     DASUDV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254635"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFV30_07855"
     CDS             <1..527
                     /locus_tag="VFV30_07855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541930.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4848042.1"
                     /translation="GGTTFRKQINRRYPDLEIQHFHHAGNSSGVVDGAAALLITSPEY
                     AKKHGLKPRARIVAYANMGDDPTLMLNAPVPAAKKVLEKAGLKKDDIDVWEINEAFAV
                     VAEKFIRDLELDRDRVNINGGAMALGHPIGATGSILIGTALDELERSGGRYGLVTMCA
                     AGGMAPAVIIERLD"
     gene            605..1987
                     /locus_tag="VFV30_07860"
     CDS             605..1987
                     /locus_tag="VFV30_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4848043.1"
                     /translation="MAQLLDEIAAIVGPQGLLTGEDVSGRAADFMGLTRIQAKALVRP
                     ASTAELSAVMKLCNAAGQVVVPAGGFTGLVQGVVCQADDIQISLERMRAIEEIDPVGR
                     TMTVEAGCPIQAVQEAAAEQGLKFPVDWGARGSATVGGGLSTNAGGNSVVRYGMMRDN
                     VLGIEAVLADGTVISSMNVLLKNNAAYDLKQLFVGSEGTLGIITRAVLKLQPAPLSTN
                     TALLAASDFAAVQRLFVHAGRRLGPALTSFEVMWASFYEPIAIQTGRHTPPLPGGHGC
                     YVIVETSGTDPESDAALFEAVLGEALEQGMAADAVIAISQTQAAQIWAIRDDIEALFH
                     YAMPSVTFDVSLPIRTMDHYVKTLEAEAAAALGETMKLVVFGHIGDGNLHILLGAQPF
                     TPETKHRIEELVYSGLRPVNGSISAEHGIGLEKRAWLGITRSPEEIALMKTIKAALDP
                     KGILNPGKVL"
     gene            complement(1982..3142)
                     /locus_tag="VFV30_07865"
     CDS             complement(1982..3142)
                     /locus_tag="VFV30_07865"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX4848044.1"
                     /translation="MAGSLNLLPASYADFRRGAERRLPRFLFDYIDGGAVSEVTLARN
                     TADWEALQLTQKVLNDASKMDCSVELFGEKLAMPLVLAPVGMGGMTARRGEVQAKRAA
                     DAAGIPFCLSTVGICPIEEVAGVSDRPMWFQLYMLKDRGIVKEILERAWNNGVRTLAF
                     TIDLPVLGTRYRDIRNGMAGGLGPWGSLRSGLIDYAIHTRWAIDVGLRGGPHGFGTIA
                     PYVNQSKNPRDYLHWTGSQFDASVTWKDIEWLRKVWQGNLVLKGVLDIDDARQSVAVG
                     ADGMIVSNHGGRQLDGVASTAAMLPRIAEAVGDSTTLLVDGGIRTGQDLVRALALGAK
                     AALIGRPWVLALGAGGEPALSRYLGLVKQDMRTALGLTGAIDARKVTGAVLL"
     gene            complement(3143..>3859)
                     /locus_tag="VFV30_07870"
     CDS             complement(3143..>3859)
                     /locus_tag="VFV30_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4848045.1"
                     /translation="FFAYPGKPSWLSPEGAALVRLASLREDAEHALGSLAEALGAPAE
                     PALVQAPTSFPAEPGKLNSASLGAILAAQLPENAIVSDEGATQGGASMLMCAGAARHD
                     WLQLTGGSIGQGLPLAVGAAVACPDRKVIALHGDGGAMYTVQALWTMARENLDVTTII
                     LNNGSYAILNIELMRVGVQNPGPKALSMLDLRNPGINWTQISQGMGVPAVRCETSEEF
                     DAALAEALAHKGPRLIEAML"
BASE COUNT          673 a         1274 c         1282 g          630 t
ORIGIN      
        1 tgggcggcac caccttcagg aagcagatca accggcgcta tcccgatctt gaaatccagc
       61 atttccacca tgccggcaac agctctggcg tggtcgatgg cgccgccgcg ctgctgatca
      121 cttcgcccga atacgccaag aagcacggcc tcaagccgcg ggcgcggatc gtggcctatg
      181 ccaacatggg cgatgatccc acgctgatgc tcaacgcgcc ggttccggcg gcgaagaagg
      241 tgctcgaaaa ggccgggctc aagaaggacg acatcgacgt ctgggaaatc aacgaggcct
      301 tcgccgtagt cgccgaaaag ttcatccgcg atctcgagct ggatcgtgac cgggtgaaca
      361 tcaacggcgg ggcgatggcg ctgggccatc cgatcggcgc aaccggatcg atcctgatcg
      421 gcaccgcgct tgacgaactg gaacgctcgg gcgggcgtta cgggctggtc accatgtgcg
      481 ccgcgggcgg catggccccg gcggtgatca tcgagcggct cgactgaacc agcccgcatg
      541 attttgccct ttgcgccccg gccgtagctg atacggtcgg ggcgttgcat ttgaaaggac
      601 acggatggcc cagcttcttg atgagatcgc cgcgattgtc ggtccccagg ggctgctgac
      661 cggcgaggac gtatcgggcc gggccgccga tttcatgggg ctgacccgca tccaggcaaa
      721 ggccttggtc cgcccggcca gcacggcaga actttcggca gtgatgaagc tgtgcaacgc
      781 cgcgggccag gtcgtggtcc cggctggcgg ctttaccggg cttgtccagg gggtggtgtg
      841 ccaggccgac gatatccaga tctcgctgga acggatgcgc gcgatcgagg agatcgatcc
      901 ggtcgggcgg accatgacgg tcgaggccgg ctgcccgatc caggcggtgc aggaagccgc
      961 ggccgagcag gggctgaagt tcccggtcga ttggggcgcg cgcggcagcg ccacggtggg
     1021 cggcgggctt tcgaccaatg ccgggggcaa ttcggtggtg cgctatggca tgatgcgcga
     1081 caacgtgctg gggatcgagg cggtgctggc cgatggcacg gtgatttcca gcatgaacgt
     1141 gctgctcaag aacaacgcgg cctatgatct caagcagctg ttcgtcggct cggaagggac
     1201 cctggggatc attacccgtg cggtgctcaa gctccagccc gcgccgcttt cgaccaacac
     1261 cgcgctgctc gcggcgagcg attttgccgc ggtccagcgg ctgttcgtcc atgccggcag
     1321 gcggcttggc ccggcgctga cctcgttcga ggtgatgtgg gccagcttct acgagccgat
     1381 tgccatccag accggacggc acaccccgcc gctgcctggt ggccacggct gctatgtcat
     1441 tgtcgagacc agcggtactg atccggaaag cgatgccgcg ctgttcgaag ccgtgctggg
     1501 cgaggcgctg gaacagggca tggcggccga tgcggtaatc gccataagcc agacccaggc
     1561 agcccagatc tgggcgatcc gcgacgacat cgaggcgctg ttccactatg ccatgccctc
     1621 ggttactttc gatgtcagcc tgccgatccg gacgatggat cactacgtca agactctcga
     1681 agccgaagct gcggccgcgc ttggcgaaac gatgaagctg gtggtgttcg ggcacattgg
     1741 cgacggcaac ctccacattc tgctgggcgc acagccgttc acgcctgaaa ccaagcaccg
     1801 gatcgaggaa ctggtctatt cggggttgag gccggtgaac ggatcgattt cggccgaaca
     1861 tggcattggc ctcgaaaaga gggcctggct ggggatcacc cgctcgcccg aagagatcgc
     1921 gctgatgaag acgatcaagg ccgcgcttga tcccaagggg atcctcaatc ccggcaaggt
     1981 gctataacaa caccgcgccc gtcaccttgc gggcatcgat cgcgccggtc aggcccagcg
     2041 cggtgcgcat gtcctgcttg accaggccga ggtagcgcga cagcgccggt tcgccgcccg
     2101 cgcccagcgc cagcacccag ggccggccga tgagcgccgc cttggcgccc agcgccagcg
     2161 cgcggaccag atcctggccg gtgcggatcc cgccatcgac cagcagggta gttgaatcgc
     2221 ccaccgcctc ggcaatccgc ggtagcatcg cggcggtcga agccaccccg tcaagctggc
     2281 gcccgccatg gttggaaacg atcatcccgt ccgcgccgac cgcaaccgac tggcgcgcat
     2341 cgtcgatatc gagcacgccc ttgagcacca ggttgccctg ccacaccttg cgcagccact
     2401 cgatgtcctt ccaggtcacc gaagcgtcga actggctccc ggtccagtgc aggtagtcgc
     2461 gcgggttctt cgactggttg acgtaagggg cgatcgtgcc gaagccatgc gggccgccgc
     2521 gcagccccac gtcgatcgcc cagcgggtgt ggatcgcata gtcgatcagg ccgctgcgca
     2581 agctacccca gggaccaagt ccgccggcca tgccgttgcg gatatcgcga tagcgcgtgc
     2641 ccagcaccgg caggtcgatg gtgaaggcca gggtccgcac cccgttgttc caggcccgct
     2701 caaggatttc cttcacgatc ccgcggtcct tgagcatgta gagctggaac cacatggggc
     2761 gatcggaaac gcccgctacc tcttcgatcg ggcagatccc cacggtcgac aggcagaacg
     2821 ggatccccgc ggcatcggcc gcgcgcttgg cctgaacctc gccgcgccgc gcggtcattc
     2881 cgcccatgcc aaccggtgcc agcaccagcg gcatggccag cttctcgccg aacagttcga
     2941 ccgagcagtc catcttcgag gcgtcgttga gcaccttctg ggtcagctgc agcgcttccc
     3001 aatcggcggt gttgcgggcc agcgtcacct cggacaccgc gccgccatcg atatagtcga
     3061 acaggaagcg cggcaggcgc cgctcggcac cgcgccggaa atcggcgtag gatgcgggca
     3121 gcaggttgag cgaaccggcc acttacagca tcgcctcgat cagtcgcggc cccttgtgag
     3181 ccagcgcctc ggcgagggcg gcatcgaatt cctcagaggt ttcgcagcgc accgcgggca
     3241 cgcccatgcc ttgcgagatc tgggtccagt tgatcccggg attcctgaga tcgagcatcg
     3301 acagcgcctt gggtccgggg ttctgcaccc ccacccgcat cagttcgatg ttgaggatcg
     3361 cgtaggaacc gttgttgagg atgatcgtgg tcacgtccag gttctcgcgc gccatggtcc
     3421 acagcgcctg gaccgtgtac atcgccccgc catcgccgtg caacgcgatc accttgcgat
     3481 cggggcaggc caccgccgcg ccgaccgcaa gcggcaaccc ctggccgatc gacccgccgg
     3541 tcagttgcag ccagtcatgc cgggccgcac cggcgcacat cagcatcgag gcgccgccct
     3601 gggtcgcacc ttcatccgaa acgatcgcgt tttccggaag ctgtgcagcg aggatcgcgc
     3661 cgagactggc cgaattgagc ttgccgggtt cggccgggaa gctggtcggc gcctggacca
     3721 gcgcgggttc ggccggagca cccagggcct cggcgagcga gccgagcgcg tgctcggcat
     3781 cttcgcgcag gctggcaagc cgcaccagcg ccgcgccttc gggcgaaagc cagctgggct
     3841 tgccgggata ggcgaagaa
//
LOCUS       DASUDV010000359         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2110384,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000359 DASUDV010000000
VERSION     DASUDV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110384"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..330)
                     /locus_tag="VFV30_07875"
     CDS             complement(10..330)
                     /locus_tag="VFV30_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX4848046.1"
                     /translation="MKHPLKPLLAAAILAAGLSFATPALADGKVTCRSGPKAQWQDIK
                     QLEAKLLREGWTIRKAKIARDCYEVYGRTPEGDNVESFHHPVSLQKILVLKRGRELFR
                     AAGY"
     gene            complement(327..902)
                     /locus_tag="VFV30_07880"
     CDS             complement(327..902)
                     /locus_tag="VFV30_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX4848047.1"
                     /translation="MRTIINSKYLFWLVLALPGLAMLRGWFSGTADAIDLLHPTGETA
                     IRLMVLALCLGPLAALVGPRGWLRWLIARRRWIGVAAFAYALAHLVCYAIDLGTLADI
                     WAEAGEHAIWTGWAALLAMAIPAATSSDPAMRLLRAGWKRAQQFAYPAAFFTVLHWGL
                     IELHWIPALLHIAPVAALNLLRWIKLKGSSR"
     gene            1003..1668
                     /locus_tag="VFV30_07885"
     CDS             1003..1668
                     /locus_tag="VFV30_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX4848048.1"
                     /translation="MNRLLILMPAVALLGACSVLPEAQAAREAPVKRSTMVFNPRPET
                     RQCLAQLGSAKASFTPLPDQYYGAGCSAVGTVKLASLRSDRRDLALSNLGPVTCPLAE
                     TFAGWARFGVDRAAQQILGSALVRIETMGSYNCRNVAGTGRRSGHATASAIDVSGFVL
                     ADGRRLTVSNDWNRNSPEVKRFFQTIHSSACKRFGMVLGPAYNAAHKDHLHLEMAARP
                     MCR"
     gene            complement(1665..2591)
                     /locus_tag="VFV30_07890"
     CDS             complement(1665..2591)
                     /locus_tag="VFV30_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848049.1"
                     /translation="MWGKPDLGDRLARALVGLAALFALGNGLFMLGDPAGWYGFVDTV
                     RATGPANPHFIRDIGLAYLTCAALLAYGAGNLAMRWGAALAGASWLALHGGLHIWEVM
                     TGLCASGIFWREAPGTLGPPLIALAGIGLQMGRQRIAPGPLPSRLFMALADKATFGLS
                     PNLGDFAAAPGHLAEKFAQFMPFSLHGHDASPQQLAFARLGAVQAEDCGPCVEIAARG
                     ALAAGIDRGEINAALAGTLPAGSAGEAYAFGRAIALNLPEVGELGDAIEARCGRAVRT
                     ELTVAAATVRVHPAFKRGLGYATSCAAHPVRI"
     gene            2662..3171
                     /locus_tag="VFV30_07895"
     CDS             2662..3171
                     /locus_tag="VFV30_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4848050.1"
                     /translation="MRLSQGVEWAAHACVILAAIGQGRALSKAALAEYHGVPAPYLAK
                     QMQLLSRAGLVRTSRGKAGGYALARPADAITLWDITRAIDGPAPLFRCTEIRQRGPCA
                     ATRADCRRPCTIAAAFAEAEAAWRAVLMRTAIADIAATVAGESRPEAVMQAVQWLGGR
                     ITALPAEQS"
BASE COUNT          558 a         1160 c         1083 g          502 t
ORIGIN      
        1 gggcgcggat cagtatcccg ccgcacggaa cagttcgcgc ccgcgcttga gcaccaggat
       61 cttctgcaag ctcaccggat ggtggaagct ttcgacattg tcgccttccg gcgtcctgcc
      121 gtagacttca tagcaatcgc gcgcgatctt ggccttgcga atggtccagc cttcgcgcag
      181 cagcttggcc tccagctgct tgatatcctg ccattgcgcc ttgggaccgc tgcggcaggt
      241 taccttgcca tcggccagcg cgggcgtggc aaacgaaagg ccggcggcaa ggatggcggc
      301 ggccagcagc ggcttgagcg ggtgtttcat cgggacgatc ctttcagttt gatccagcga
      361 agcaggttga gcgccgccac cggggcgatg tgcagcagcg cagggatcca gtgcagttcg
      421 atcaggcccc agtgcagcac ggtgaagaag gcggccggat aggcaaattg ctgcgcccgc
      481 ttccagccgg cgcgcagcaa gcgcatcgcc gggtcggaac tggttgcagc cgggatcgcc
      541 atggccagca gggcggccca gccggtccag atcgcgtgtt cgccggcttc ggcccagata
      601 tcggccaggg tccccagatc gatcgcatag cagacgaggt gcgccagggc ataggcgaag
      661 gccgccaccc cgatccagcg ccgccgggcg atcagccagc gcagccagcc gcgcggcccc
      721 accagcgccg ccagcggccc caggcatagc gccagcacca tcaaccggat cgcggtttca
      781 cccgtgggat gcagcaggtc gatcgcatcg gcagtgccgc tgaaccagcc gcgcagcatg
      841 gccagaccgg gcagcgccag cacgagccag aacagatatt tgctattgat aatcgttcgc
      901 attaaaccgg cgttagcctg tgatcgtggc tgttgcaaga cccgccgcaa cgcccttgcc
      961 cgctaagaga aaattaacca tcccggcctg acaattgctg ccatgaaccg cttgctcatc
     1021 ctgatgcccg cggtggccct gctcggcgcc tgttcggtgc tgcccgaggc gcaggcagcg
     1081 cgcgaggcac cggtcaagcg ttcgaccatg gttttcaatc cgcggcccga aacccgccag
     1141 tgcctggccc aactcggatc ggccaaggcc agtttcactc ccctgcccga tcagtattac
     1201 ggcgccggtt gctcagccgt gggcacggtc aagctggcca gcctgcgcag cgacaggcgc
     1261 gatctggcgc tgagcaatct tggcccggta acctgtccgc tggccgaaac ctttgccggc
     1321 tgggcccgtt tcggggtcga ccgcgcagcg cagcagatcc tgggttcggc gctggtccgg
     1381 atcgagacca tgggcagcta caactgccgc aacgtggccg gtaccgggcg gcgatcgggc
     1441 catgccacgg ccagcgcgat cgacgtttcg ggctttgtcc tggccgacgg ccggcggctc
     1501 accgtatcga acgactggaa ccgcaattcg cccgaggtga agcgcttctt ccagaccatc
     1561 cattccagcg cctgcaagcg cttcggcatg gtgctgggcc cggcctacaa tgccgcgcac
     1621 aaggaccacc tgcatctgga gatggccgcg cggccgatgt gccgttagat ccgcaccgga
     1681 tgcgccgcgc agctggtggc atagcccagc ccgcgcttga acgccgggtg gacccgcacg
     1741 gtcgccgcgg ccacggtcag ctcggtgcgc accgcccggc cgcagcgcgc ctcgatggca
     1801 tcgccaagct cgcccacctc gggcaggttg agtgcgatgg cacggccaaa ggcataggct
     1861 tcgcccgcgc tgcccgccgg cagcgtgccc gccagcgcgg cgttgatctc gcccctgtca
     1921 atccccgcag ccagcgcccc gcgcgccgcg atctccacgc aggggccgca atcctcggcc
     1981 tggaccgccc ccagccgggc gaaggccagc tgctgtgggc tggcatcgtg accgtgcagc
     2041 gaaaagggca tgaactgggc gaacttttcc gccaggtgtc ccggcgcggc ggcaaaatcg
     2101 ccaaggttgg gcgacaggcc gaaggtcgcc ttgtcggcca gggccatgaa caatcgcgag
     2161 ggcaagggac ccggcgcgat ccgttgccgg cccatctgca agccgatgcc cgccagtgcg
     2221 atcaggggcg ggcccagcgt accgggagcc tcgcgccaga agatgccgct ggcgcacagc
     2281 ccggtcatca cctcccagat gtgcaggccg ccgtgcagcg ccagccagct cgccccggcc
     2341 agcgccgcgc cccagcgcat cgccaggttg ccggcaccat aggccagcag cgccgcgcag
     2401 gtcagatagg cgaggccgat atcgcggatg aaatgcgggt tggccgggcc tgtcgcccgg
     2461 acggtatcga caaagccgta ccatccggcc ggatcgccca gcatgaacag accgttgccc
     2521 agcgcgaaca gtgccgcaag gcccaccagt gcccgcgcca ggcgatcgcc caagtccggt
     2581 tttccccaca ttgtgctcgc tccttgcctg tccgactcaa ttcggatatt atttatccgc
     2641 attagaaagg atcggcaagc catgcgattg agccaggggg tggaatgggc cgcgcacgcg
     2701 tgcgtgatcc tggccgcgat tggccagggc cgcgcgctga gcaaggcggc gctggccgaa
     2761 tatcatgggg ttcccgcccc ctatctggcc aagcagatgc agctgctgag ccgggccgga
     2821 ctggtccgca ccagccgggg caaggccggc ggctatgccc ttgcccgccc ggccgatgcg
     2881 atcaccctgt gggacatcac ccgcgcgatc gacggtcccg cgccgctgtt ccgctgcacc
     2941 gagatccgcc agcgcggacc ctgcgcggcg acgcgagcgg actgccgccg cccctgcacc
     3001 atcgccgccg cctttgccga ggccgaggcc gcctggcgcg cggtgctgat gcgcaccgcc
     3061 atcgccgata tcgccgcaac ggtcgccggc gagtcgcggc ccgaggccgt catgcaggcg
     3121 gttcagtggc ttggcgggcg cataaccgcg ctgcccgccg aacaatcctg acagattcat
     3181 tgcaactatc agcaaatgcc cccttggcgc cgcgctggcg gcaggctaat ccttttcgca
     3241 actgcagcga gaaggggtgc tgctcgggcc gaacggaaaa gccgtcggcc ttcgtcagga
     3301 gga
//
LOCUS       DASUDV010000360         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1469978,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000360 DASUDV010000000
VERSION     DASUDV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469978"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFV30_07900"
     CDS             complement(<1..1193)
                     /locus_tag="VFV30_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX4848051.1"
                     /translation="MADTRKPKPAVTRIGNHELSPATLMMGHGYDPVLSEGSLKAPIF
                     LTSTFAFPSAADGKRFFEGITGKVPGGTDGLVYSRFNGPNQQILEGRLAVWDGAEEAL
                     SFSSGMTAICVLFLAYCNAGDVIVHSGPLYAASEGFVAKTLSRFGVTYVDFPAGATRE
                     ELDAVLAKAKAQAAAQGGKVAMIYLESPANPTNALVDVEAVRDARDAALGADCPIAID
                     NTFLGPLWSRPIDHGADIVVYSLTKYVGGHSDLVAGSVSGAKKWMTPVRMLRNTMGGI
                     VDPNTAWMLMRSLETVELRMSRAGENAAKVCAWLAQHPKVEKLGYLGMIDDPRQQDIY
                     DRHCTGAGSTFSLYIKGGEAEAFRFLDNLKIAKLAVSLGGTETLASLPAAMTHLSVPD
                     ERKAQL"
     gene            complement(1375..2724)
                     /locus_tag="VFV30_07905"
     CDS             complement(1375..2724)
                     /locus_tag="VFV30_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX4848052.1"
                     /translation="MLTAAPAGMPDAAWPTAGLVWWMAAWWMTEALPLSVTALLPFIV
                     LPMVGVADANKTASTYYSPVMFLFLGGAFLALAIERTGLHRRLALAILARAGHSPLRL
                     LLAVMCATALISMFISNTSTALIMMPMALAMLASGGVREGETLGMAGALPMGVAFAAT
                     LGGYGTIVGTPTNAIAVALLQKSLGVEISFVEWSMYGLPLVALSVPLAALIIARVQRI
                     GESSFDPAAARAAIHLPPAWTTPEKRLAPIFAVTVLAWVTMPLLKPLFPPGGLDDGTI
                     AALAGLTLFFIPDGTGRPLLKWDEANRAPWGVILMFGGGLALAMGMTESGLADWMGQM
                     LLPLKAVPLPIVALVLVGFVVLVTEFASNIAAASGIMPVVGALVVALGADPILLALPA
                     ALAASWGFMLPAGTGPNALAWATGHIALPRVLKAGLMLDLAGVPLMVGVVWAVAALT"
BASE COUNT          480 a          915 c          951 g          395 t
ORIGIN      
        1 agctgcgcct tgcgctcgtc cggcaccgaa aggtgcgtca tcgcggcggg caggctggcg
       61 agggtctcgg ttccgcccag gctgaccgcc agcttggcga tcttgaggtt gtcgagaaag
      121 cggaacgcct cggcctcgcc gcccttgatg tagagcgaga aggtgctgcc cgccccggtg
      181 cagtgccggt catagatatc ctgctggcgc ggatcgtcga tcatgccaag gtagccgagc
      241 ttttcgacct tgggatgctg ggcaagccag gcgcatacct tggcggcgtt ctctcccgcg
      301 cggctcatgc gcagttcgac tgtctcgagg ctgcgcatca gcatccaggc ggtgttggga
      361 tcgacgatcc cgcccattgt gttgcgcagc atgcgcaccg gggtcatcca cttcttcgcg
      421 cccgaaaccg agccggcgac gaggtcggag tgcccgccga cgtacttggt cagcgaatag
      481 acgacgatat cggcgccgtg gtcgatcggg cggctccaga gcgggccgag gaaggtgttg
      541 tcgatcgcga tcgggcagtc cgcgcccagc gccgcgtcgc gcgcgtcgcg cacggcctcg
      601 acatcgacca gcgcgttggt cgggttggcg gggctttcaa ggtagatcat cgccaccttg
      661 ccgccctgcg cggcggcctg cgccttggcc ttggccagca ccgcgtccag ctcctcgcgg
      721 gtcgcaccgg cggggaagtc gacataggtc acgccgaagc ggctgagcgt cttggcgacg
      781 aagccctcgc tggccgcgta aagcgggccg gaatggacga tcacgtcacc ggcattgcaa
      841 taggccagga acagcacgca gatcgcggtc attccgcttg aaaagctcag tgcttcttcc
      901 gcgccgtccc acaccgccag gcggccttcg aggatctgct ggttggggcc gttgaagcgc
      961 gaatagacca ggccgtcggt gccgcccggg accttgccgg tgatcccttc gaagaagcgc
     1021 ttgccgtcgg cagcgctggg gaaggcaaag gtgctggtca ggaagatcgg ggccttgagg
     1081 ctcccttccg acagcaccgg atcataaccg tggcccatca tcagggtggc gggcgagagt
     1141 tcgtgattgc cgatgcgggt gacggcgggc ttgggcttgc gggtatcggc catgaaaggc
     1201 gtccttcctg aaacggcctc gtccagcgag gcgcggaacg ctcccaggaa ggcgggagct
     1261 ggtctggcag cgggggccct ggcgccccta acctccgcag gtgcgcatct ccgcccggtg
     1321 ccgtgctggc ggcaccctaa cggcgggcaa aactgatttc aactgcaacc ggaatcaggt
     1381 cagcgcagcg accgcccaga cgaccccgac catcagcggc acacccgcca ggtcaagcat
     1441 cagcccggcc ttgagcacgc ggggcagggc aatgtggccg gtcgcccaag ccagggcatt
     1501 cgggccggtg ccggccggca gcatgaagcc ccatgaagca gccagcgcgg ccggcagggc
     1561 cagcaggatc gggtctgccc ccagcgccac caccagcgcg ccgaccaccg gcatgatccc
     1621 gctggcggcc gcgatattgc tggcgaactc ggttacgagg accacgaacc cgaccagcac
     1681 cagggcgaca atcggcagcg gcacggcctt gagcggcagc agcatctggc ccatccagtc
     1741 agccaggccg ctctcggtca tgcccatggc cagggcgagg ccgcccccga acatcaggat
     1801 cacgccccag ggcgcgcggt tggcctcatc ccacttgagc aaggggcggc cggtgccgtc
     1861 gggaatgaag aacagcgtca gcccggccag cgcggcgatg gtgccgtcgt caagcccgcc
     1921 cggcgggaac agcggtttca gcagcggcat ggtcacccag gccagcacgg tcacggcaaa
     1981 gatcggcgcc aggcgttttt cgggcgtggt ccaggccggt ggcaagtgga tcgcggcgcg
     2041 ggcggcggcg ggatcgaaac tgctttcgcc gatccgctgg acccgggcga tgatcagcgc
     2101 ggcgagcggg acggacagag ctaccagtgg caagccatac atcgaccatt cgacaaagct
     2161 gatctccacc cccaggctct tctggagcag ggcgacagcg atggcattgg tcggggtgcc
     2221 aacgatcgtg ccatagccgc ccagcgtcgc ggcaaaggcc acgcccatcg gcagggctcc
     2281 ggccatgccc agcgtctcgc cctcgcgcac cccgcccgag gcgagcatgg ccagcgccat
     2341 cggcatcatg atcagcgcgg tggaggtgtt ggagatgaac atgctgatca gcgccgtggc
     2401 gcacatcacc gccagcagca gtcgcagcgg cgaatggccg gcgcgcgcca ggatcgccag
     2461 cgccagccgg cggtgcagcc cggtccgctc gatcgcgagc gccaggaagg ccccgcccag
     2521 gaacaggaac atgaccggcg agtaataggt cgaggcggtc ttgttggcat cggccacgcc
     2581 gaccatcggc aggacgatga acggcagcaa cgctgtgacc gacagcggca gcgcctcggt
     2641 catccaccag gctgccatcc accagactag cccggcggtc ggccaggcgg cgtcgggcat
     2701 cccggcagga gcagctgtca gcagagtgaa gaggaagccg a
//
LOCUS       DASUDV010000361         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_740641,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000361 DASUDV010000000
VERSION     DASUDV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740641"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..798)
                     /gene="pyrF"
                     /locus_tag="VFV30_07910"
     CDS             complement(121..798)
                     /gene="pyrF"
                     /locus_tag="VFV30_07910"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX4848053.1"
                     /translation="MSTNPIYLALDFPRLETAIEVARKASAHIGGVKLGLEFFCAHGH
                     HGVHEVHKLGLPIFLDLKLHDIPNTVAGAMQAIHVLEPAIVTIHAAGGRAMMEDAKAA
                     AGENTKVVAVTLLTSLDEADMAATGMVGQPHDQVMRLAELSRDAGLDGIVCSGHEVAA
                     VHSMWRDGYLVVPGLRPADAKLGDQKRAVTPRQARDAGASVLVIGRPIARAEDPAAAA
                     RAIEATL"
     gene            complement(795..1139)
                     /locus_tag="VFV30_07915"
     CDS             complement(795..1139)
                     /locus_tag="VFV30_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX4848054.1"
                     /translation="MQLIRTLLLLLVTIILVAFIAINWQTVPLNLWPLQDGNYLHLEW
                     PVGFIVLVSMTLGFLPMWLLHKGVRWRLNRRISLLETTMKSAATTPGAPTAVATTTQL
                     EAAAQEPPANPS"
     gene            complement(1161..2153)
                     /locus_tag="VFV30_07920"
     CDS             complement(1161..2153)
                     /locus_tag="VFV30_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848055.1"
                     /translation="MKRVLKWILGILLVALIGLFAVGYTPDTDPAAMRLKYGGQAARF
                     MDVGGGVTVHYRDQGNPQGPVLVLLHGSNASLHTWEPWVARLQGKYRIISLDQIGHGL
                     TGPNPTGKYDSAAFVGTLDALMTKLGITRFALAGNSMGGYVAWEYALAHPDKLTHLVL
                     VAAAGAPEDPNREIPIGFKIARHPLLSKLGLVITPRGMFERSLHGSVSNHAVITDAVI
                     DRYWELNRMPGNRAATGARFAQYGTRQNNTARLGEIKVPTLILWGAEDKLLPVSGAGW
                     FAAQIPGSVRIVYPKIGHMPQEETADRSAADADAFLSKPFPSPGSATSIPAPRP"
     gene            complement(2166..>2414)
                     /locus_tag="VFV30_07925"
     CDS             complement(2166..>2414)
                     /locus_tag="VFV30_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX4848056.1"
                     /translation="HSQRVLLALTQAGLERDASYRLVQRNAMKVWESDGQLSLLELLK
                     ADPEVSAVMSAAELEEKFNLDYHFKAIDTIFDRVFGPQ"
BASE COUNT          383 a          773 c          842 g          416 t
ORIGIN      
        1 tcttcgggac tgagcgaggg cgcgtcggtg atggtgaccg cctgaacggt tccggccagg
       61 cccgctgcca gcacggcacc aagggcaatt cgggcgatct tcgtcatggc tgtcctcggt
      121 tcaaagggtg gcctcaatcg cgcgggccgc ggcggcgggg tcttcggcgc gggcgatcgg
      181 gcggccgatc accagcacgc tggccccggc atcgcgggcc tggcgcgggg ttacggcgcg
      241 cttctgatcg cccagcttgg cgtccgccgg acgcaggccc ggcaccacca gatagccatc
      301 gcgccacatg gagtgaacgg cggcgacctc gtgccccgaa cagacgatcc cgtcgagccc
      361 ggcatcgcgc gacagttcgg cgaggcgcat gacctggtcg tgcggctgtc cgaccatgcc
      421 ggtggcggcc atgtcggcct cgtccaggct ggtcagcagg gttacggcaa cgaccttggt
      481 gttctcaccc gcggcggcct tggcgtcttc catcatcgcc cgtccgccgg cggcgtggat
      541 cgtcacgatc gccggttcca gcacgtggat cgcctgcatc gccccggcca cggtgttggg
      601 aatgtcgtgc agcttcagat cgaggaaaat cggcaggccc agcttgtgaa cctcgtgcac
      661 gccgtggtgg ccgtgggcgc agaagaattc cagccccagt ttgaccccgc cgatgtgggc
      721 ggacgccttg cgggccacct ctatcgcggt ttccaggcgg ggaaaatcaa gcgcaagata
      781 gatcgggttg gtgctcatga cgggtttgcc ggcggctcct gcgctgctgc ttcaagctgg
      841 gtggtggtgg cgacggcggt gggcgcgccg ggggtggtgg cggcgctctt catcgtggtt
      901 tccaacaggc tgatccgccg gttcaggcgc cagcgcacgc ccttgtgcag cagccacatt
      961 ggcaggaagc ccagcgtcat cgaaaccagg acgatgaatc cgaccggcca ttccaggtgc
     1021 aggtagttgc catcctgcag cggccagagg ttgagcggaa ccgtctgcca gttgatcgcg
     1081 atgaacgcga ccaggatgat ggtgacgagc agcagcagca gcgtgcggat cagttgcatg
     1141 gcgcggaccc tcctcagcaa tcaaggtctt ggagcgggga tgctagtggc agagccgggc
     1201 gaagggaagg gcttcgacag gaaagcgtcg gcatcggcgg cggaccggtc ggcggtctct
     1261 tcctgcggca tgtgcccgat cttcggatag acgatccgga ccgatccggg gatttgcgcg
     1321 gcgaaccagc cggccccgct gaccggcagc agcttgtcct ccgcgcccca caggatcagc
     1381 gtcggcacct tgatttcgcc caggcgcgcg gtgttgttct ggcgcgtgcc gtactgggca
     1441 aagcgggcgc cggtggccgc gcggttgccg ggcatccggt tcagctccca gtaacggtcg
     1501 atcacggcgt cggtgatcac cgcgtggttc gagaccgagc cgtggagcga gcgttcgaac
     1561 atgccgcgcg gggtgatcac cagccccagc ttgctgagca ggggatggcg ggcaatcttg
     1621 aagccgatcg ggatttcgcg gttcggatcc tccggcgccc cagccgcggc gaccagcacc
     1681 aggtgggtca gcttgtccgg atgggccagg gcatattccc aggcgacata gccgcccatc
     1741 gaattgccgg cgagcgcgaa gcgggtgatg cccagcttgg tcatcagcgc atccagcgtg
     1801 ccgacgaagg cggcgctgtc atacttcccg gtcgggttcg ggccggtcag gccgtggccg
     1861 atctggtcca ggctgatgat ccggtatttg ccttgcagcc gcgccaccca cggctcccag
     1921 gtgtggagcg aggcgttgga gccgtggagc agcaccagca ccggcccctg ggggttgccc
     1981 tggtcgcggt agtggaccgt taccccgccg cccacatcca tgaaccgcgc cgcctggccg
     2041 ccgtatttca ggcgcatcgc cgccggatcg gtatcggggg tatagccgac cgcaaacagg
     2101 ccgatcagcg cgacgagcag gatcccgagg atccatttaa gaacgcgctt catggcggca
     2161 agctcctatt gcgggccgaa cacccggtcg aaaatcgtgt cgatcgcctt gaagtggtag
     2221 tcgagattga acttctcttc cagttcggcc gcgctcatca ccgcgctaac ttccggatcg
     2281 gccttgagca gttccagcag gctcagctgc ccgtccgatt cccacacctt catcgcgttg
     2341 cgctgaacca gccggtagga ggcgtcgcgc tccagcccgg cctgggtcag ggcgaggagg
     2401 acgcgctgcg agtg
//
LOCUS       DASUDV010000362         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1293566,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000362 DASUDV010000000
VERSION     DASUDV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293566"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..809
                     /locus_tag="VFV30_07930"
     CDS             66..809
                     /locus_tag="VFV30_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX4848057.1"
                     /translation="MRLHPLLVPVADLVFPPRCPLCGEAIAAQSGLCAACWSELAIPG
                     APCCPSCQRPLGERTPDGTVCAPCLAEPPAHDGIAAGTLYNPASRKLILALKHGHRIA
                     LAPLLARLMLAQVPEEVGPDWLVVPVPLHRWRLWRRGFNQAALLGRELAAARGAELVV
                     DALVRRKRTPTLGGLGKSARARALAGAIAVHPRRAGRIKGAKVLLVDDVLTSGATSNA
                     SVKALRRAGAERVVVACFARVLDEALDLV"
     gene            1069..1446
                     /gene="hisI"
                     /locus_tag="VFV30_07935"
     CDS             1069..1446
                     /gene="hisI"
                     /locus_tag="VFV30_07935"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX4848058.1"
                     /translation="MTDHDPADRERGTPFLPKFDAQGLLVAIAQDQTTREILMVAYMD
                     AEALAKTRETGLAHFHSRSRGRLWLKGETSGHVLRVAEIRADCDQDALVLLVSPEGPA
                     CHTGATSCFYRKLDGAELSGLSD"
     gene            1479..1925
                     /locus_tag="VFV30_07940"
     CDS             1479..1925
                     /locus_tag="VFV30_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4848059.1"
                     /translation="MDQLGLSAPTHDGAHIDTPDALDREQFTISDLTGEFGVTARALR
                     FYEDEGLIAPARIGLSRVYSKRDRARLAWILRAKNVGFSLTEIKEMIDLYDLGDGRVE
                     QRRVTVQRCKEKVERLSQQRADLDAAITELTQFIKMVEEIDLARKG"
     gene            1949..>3255
                     /locus_tag="VFV30_07945"
     CDS             1949..>3255
                     /locus_tag="VFV30_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4848060.1"
                     /translation="MPTYQAPTRDTRFVINEVLNLESYGHLPGFENASADLVDAVVEE
                     CGKFCSEVLAPLNQSGDQEGCTRHPDGSVTTPKGFKEAHAQFREAGWGTLSAPEQFGG
                     QGMPHVLGMMAEEFISSANQAWGMYPGLTGGAIASILVKGSPEQQAKYLPKMIANEWL
                     GTMNLTEPHCGTDLGLIRTRAVPNADGSYAITGTKIFISAGEHDLTENIIHLVLAKTP
                     DAPDSTKGISLFIVPKVLVNDDGSLGARNAVSCGSIEHKMGIHGNATCVMNYDGATGF
                     MVGEENKGLAAMFIMMNAARLGVGIQGLSQAEAAYQNAVKYAQDRRQGRALTGPAEPN
                     EKADTLFVHPDIRRMLMDAKAFTEGMRALSLWGALQVDLVHQAASEEERQMADDLISL
                     LTPVIKGYGTDKGFDTCVNMQQVFGGHGFIEEWGMSQFVRDCR"
BASE COUNT          568 a         1068 c         1103 g          516 t
ORIGIN      
        1 cgggctgggt cacgccgctg ccactgcctt gcgccgcgcg ggcttgcaag cgataccgga
       61 gcggaatgcg cctgcacccg ctcctcgtgc ccgtggccga tttggtcttc cccccgcgct
      121 gcccgctgtg cggggaagcg atcgcggcgc agtccggcct ctgcgcggcc tgctggagcg
      181 agctggcgat ccccggcgcg ccctgctgcc ccagctgcca gcgcccgctg ggcgagagga
      241 cgccggacgg cacggtctgc gcgccctgcc tggccgagcc gccggcacat gacgggatcg
      301 cggccggcac gctttacaac ccggcctcgc gcaagctgat cctggcgctg aagcacggcc
      361 accgcatcgc gctggccccg ctgctggcgc ggttgatgct ggcgcaggtg cccgaagagg
      421 tcggaccgga ctggctggtc gtgccggtgc cgctccaccg ctggcggcta tggcggcgcg
      481 gtttcaacca ggccgcgctg ctgggccgcg agctggccgc ggcgcgcgga gcggagctgg
      541 tggtcgatgc cctggtccgg cgcaagcgga caccgaccct gggcggactc ggcaagtccg
      601 cgcgggcccg cgccttggcc ggggcaatcg cggtccatcc ccgccgcgcg ggccggatca
      661 agggcgcaaa agtgctgctg gtcgacgacg tgctgaccag cggggcgacc agcaacgcat
      721 cggtcaaggc gcttcggcgg gccggggcag agcgggtggt ggtggcctgc tttgcgcggg
      781 tgctggacga agcgctggat ctggtctgag ggccaaacga aaacgcccgg aaccgaagct
      841 ccgggcgtcc tcgtgacgaa atctgcaggg cttccgctat tgcggttcgg cacacccccc
      901 taggaatgcc tcgccctaat ccctcatgcg gcaagcggtc cctgaacctg ggccatcagc
      961 ctttccgccc ggtctcaatc cccgaatccg cccggcaagg aaagggccaa gccgcgaggg
     1021 gcgaattttt gctcgacaga tgtggttatc ttgcaacaag gcatgcccat gaccgatcac
     1081 gatcctgccg accgcgaacg gggcactccc ttcctgccca agtttgacgc ccaaggcctg
     1141 ttggtggcca ttgcccagga ccagaccacc cgcgagatcc tgatggttgc ctatatggac
     1201 gccgaggcgc tggccaagac ccgagagacc gggctggctc atttccactc gcgctcgcgc
     1261 gggcggttgt ggctcaaggg cgagacttct ggccacgtgc tgcgggtggc ggaaatccgg
     1321 gctgactgcg atcaggacgc gttggttctg ctggtttcgc cagaagggcc ggcctgccac
     1381 accggcgcga ccagctgctt ctaccgcaag ctggacggag cggaactgag cggcctgtca
     1441 gattgacgct tacgtaaagg taagctaaag ccgcggccat ggatcagctc ggcctcagcg
     1501 cgccgactca tgacggcgct cacatcgata caccggacgc gctcgaccgc gagcagttca
     1561 cgatttcgga cctgaccgga gagttcgggg tcaccgcccg ggcgctgcgg ttctacgagg
     1621 acgaaggcct gatcgctccg gcccggatcg gtctcagccg cgtctattcg aagcgcgacc
     1681 gggcccgcct ggcctggatc ctgcgcgcca agaacgtcgg cttcagcctg accgagatca
     1741 aggaaatgat cgacctttac gatcttggcg atggccgggt cgagcagcgc cgcgtcacgg
     1801 tgcagcgctg caaggagaag gtcgagcggc tcagccagca gcgggccgat cttgacgccg
     1861 cgattaccga gctgacccag ttcatcaaga tggtcgagga aatcgacctc gcccgcaagg
     1921 gctgataccc ccaacgaagg attgcttcat gccgacctat caggcgccga cgcgcgacac
     1981 ccgtttcgtt atcaacgaag tgctcaacct cgaaagctat gggcatctcc ccggctttga
     2041 gaacgccagc gccgatctgg tcgatgcggt ggtcgaggaa tgcggcaagt tctgttccga
     2101 ggtgctcgcc ccgctcaacc agtcgggcga ccaggaaggc tgcacccggc atcccgatgg
     2161 ttcggtgacc acgcccaagg gtttcaagga agcccatgcc cagttccgcg aagcgggctg
     2221 ggggacgctg tcggctccgg agcagttcgg cgggcagggc atgccgcacg tgctggggat
     2281 gatggccgag gaattcattt cctctgccaa ccaggcctgg gggatgtatc ccggcctgac
     2341 gggtggggcg atcgcctcga tcctggtcaa gggcagcccg gaacagcagg ccaagtacct
     2401 gcccaagatg atcgccaacg aatggctcgg cacgatgaac ctgaccgagc cgcactgcgg
     2461 caccgacctg ggcctgatcc gcacccgcgc agtgccgaat gccgacgggt cctatgcgat
     2521 caccggcacc aagatcttca tttcggccgg tgagcacgac ctgaccgaga acatcatcca
     2581 tctggtcctg gccaagaccc cggatgcgcc cgacagcacc aagggcatct cgctgttcat
     2641 cgtgcccaag gtgctggtga acgacgacgg ctcgctgggt gcgcgcaacg cggtgtcgtg
     2701 cggcagcatc gaacacaaga tggggatcca cggcaacgcc acctgcgtca tgaactatga
     2761 cggcgcgacc ggtttcatgg tgggcgagga gaacaagggc ctggccgcga tgttcatcat
     2821 gatgaacgcg gcgcgcctgg gcgtgggcat ccagggcctg agccaggccg aggcggccta
     2881 tcagaacgcg gtgaagtatg cccaggaccg gcgccagggc cgcgcgttga ccggaccggc
     2941 cgagccgaac gagaaggccg ataccctgtt cgtgcacccc gatatccggc gcatgctgat
     3001 ggacgccaag gcctttaccg aagggatgcg ggcgctcagc ctgtggggcg cgctgcaggt
     3061 tgacctggtt caccaggcgg ccagcgagga agagcgccag atggccgatg atctgatcag
     3121 cctgctgacc ccggtgatca agggctatgg caccgacaag ggcttcgaca cctgcgtcaa
     3181 catgcagcag gtgttcggcg gccacggctt catcgaggaa tggggcatga gccagttcgt
     3241 gcgcgattgc cgcat
//
LOCUS       DASUDV010000363         8802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1602634,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000363 DASUDV010000000
VERSION     DASUDV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602634"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFV30_07950"
     CDS             complement(<1..789)
                     /locus_tag="VFV30_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4848061.1"
                     /translation="MSHLLDRLTFFRQKKEQFSEGHGVTTAEARDWEDSYRKRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIITWETQQTDYPRTRPELPNHEPRGCPRGASYSWY
                     IYSAQRLKYPLIRKRLVKLWRAARKDLPPVAAWASIQADPVKRKSYTAIRGHGGFVRS
                     TWDEVNEIIAAANAYTAKQYGPDRVIGFSPIPAMSMVSYAAGSRYLSLLGGTCMSFYD
                     WYCDLPPASPMTWGEQTDVPESADWYNAGFLMLWGSNVPQTRTPD"
     gene            complement(786..3524)
                     /locus_tag="VFV30_07955"
     CDS             complement(786..3524)
                     /locus_tag="VFV30_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848062.1"
                     /translation="MPLSQTVSSGQQNRALGLSTFAFTICFAVWTIFAIIGIEIKAEL
                     GLNDTQFGLLVGTPILTGSLVRLFLGVWTDQYGGRIVFPLTMLASAVSTVLLSQADSY
                     ILMLLAALGLGMAGGGFAVGVAYVSKWYPQEKQGSALGFFGMGNVGAAVTKFVAPFVM
                     VALGWQAVAQIWAVVLAITAVLFFLFAKDDPDLAARKISGEKPKGLKEQLEPLRNEQV
                     WRFSLYYFFVFGAFVALALWLPKYLIGVYGVDIKVAGMLAATFSLSASVFRAYGGILS
                     DRYGARRIMYATFGVSMLCLFMLSYPATDYTIHGIKGDIRFSTSMSLVPFVITVFVLG
                     FFMSLGKAAVYKHIPVYYPGHVGSVGGLVGLVGGLGGFVLPIVFGAVSDLTGIWTSCF
                     MVLFALVAVALGWMHLAIRQMEQKASGIDQRSLPQFPEMAGLHDAASHGEQAVKGKVL
                     TDWRPEDSAFWEETGQRIANRNLYLSVPALLLAFAVWMVWSVVVAKLPLIGFDYTTDQ
                     LFWLAALPGLSGATFRIFYSFMVPIFGGRLWTTLSTASLLIPAFGIGYAVQDPDTPYF
                     IFLVLALLCGFGGGNFASSMSNIGFFFPKAQKGNALALNAGLGNLGVSVMQFAVPLVI
                     TAGVFGALGGTAQVAKDGSELWLQNAGFIWVPFIIASTLLAWFGMHDIADAKASFAEQ
                     AVIFQRKHNWLMCWLYTGTFGSFIGFSAGLPLLAKSQFPDVNVLKYVFLGPLVGAVSR
                     AATGWIADRWGGARVTFWVFIGMIVGALGVIHFLEVKDFNGFLAAFIFMFFVTGVGNA
                     STFQMIPNIMRQEVPRLMPELDSAHRTRQAEKESAAIVGFTSAIAAYGAFFIPRAFGM
                     SISATGTPLAALYGFIVFYASCAALTWLIYTRKGGLLHDVERGRAPTAPIAQGATA"
     gene            3740..4435
                     /locus_tag="VFV30_07960"
     CDS             3740..4435
                     /locus_tag="VFV30_07960"
                     /inference="COORDINATES: protein motif:HMM:NF006901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4848063.1"
                     /translation="MRPGERPEIANLPLFREMQDGQRDRIFSGSFLQAFPPQLTLFEL
                     GQHPDFLHVLVDGLVELYAHSAGRDTTMRIVEPVTSFILAAVITDQPYLMSARTIAPS
                     RILLVPAALVREVVKDDCALMQATMRELAQAYRDMVRALTDMKLRQSAERLGNLILQQ
                     ERRQGGSGNVQLKAEKRLLASLLGMTPENLSRAFGVLSGHGIAVSGAQVTITDRAALE
                     AFSRPDPVRDDLA"
     gene            complement(4436..5641)
                     /locus_tag="VFV30_07965"
     CDS             complement(4436..5641)
                     /locus_tag="VFV30_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEX4848064.1"
                     /translation="MNQASSRIALTLAALLASTAAHAETQPVTPYVDLRYRLELVDQD
                     GLAEQAAASTLRVKAGIKTAEWHSFSAVVEGEAIVVLGETSYNDTVNGKTAYPVVADP
                     SDVLLNQAYLRWRPMAELEMVGGRQAVNFDNQRWIGSVGWRQNDQTLDTVRVTARPVK
                     GASVDYFYAWRVNRVFGPDSPQGIWRDTKIHGVRAAYTIKNVGTLSAYGYWLDVPSSP
                     ASSNRTLGVRMAGELPVGGKASILYSAEYARQHDYGSNPQSFGLDYWLFEPGVKAGPV
                     TLKAGYERLEGNGTVGLQTPLATLHAFNGWADKFLTTPASGLRDVYLDAGYKVPGKGP
                     LAGLVLRAAWHDYGSTTGGIPYGQEWNALASYPLGKRFTLTAKLAHYDADAFASDTTK
                     LWFQIEAKF"
     gene            5731..6195
                     /locus_tag="VFV30_07970"
     CDS             5731..6195
                     /locus_tag="VFV30_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848065.1"
                     /translation="MDTFTLARGLHVVAIVGWIGGVWFVTLVVMPAIVRSEPPANRLS
                     AFHRIEQGFAPQAKFWVLLAGASGFWMTHQADLWGRFADPAFWWMHAMLGLWTIFAVM
                     LFVAEPLFLHRRMARSATPEADWARMVTMHRLLSAIALVTVIGATGGAHGLF"
     gene            6195..7397
                     /locus_tag="VFV30_07975"
     CDS             6195..7397
                     /locus_tag="VFV30_07975"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX4848066.1"
                     /translation="MTSPAPLLLSAPHRLAFLTGSLGLTAMALWWPLRLAALHLGWSE
                     PPTGGLPAALLHGPVLLLHVYPAFIFGFLLTVFPRWMGQPDLDARRFGPISAGLAAGM
                     AATTIGLWTGSDAAVRTGFTIVAAAWALGLFVLAVVVRANGRSGKPPCWHAVSALAAL
                     GLGLGALGLVAAFLFGGDPRLLRWGNVLGLNLFILPVFLTVAHRMVPFFAGNVVTGYR
                     RWRPDWLLAALWTLLVLRIAGELALVPALSGLAATGLAACTGLMAWKWWPRGPAPGLM
                     QVLIWGFAWAPLGFALSALASLGLPLGLAPTHALALGFAASLLVAMVTRVTHGHSGRP
                     LAMVPLAWLAFAAVQLAAGLRIVAAIKGEPGVLLVSGALALAAGLLPWLIRNALIYLR
                     PRLDGRDG"
     gene            7381..7998
                     /locus_tag="VFV30_07980"
     CDS             7381..7998
                     /locus_tag="VFV30_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX4848067.1"
                     /translation="MAAMDSPPQITPRAALELLKEGNRRFLSDAPYNPPMDRLRRLHL
                     AAAQRPFAAYLSCSDSRVPPELLFERGLGELFIVRNAGNTLCATALGSLEFAVTQLQV
                     PLIVVMGHEACGAIRAAVSVARGETAYTGNVSKVLQSLLPAVLEADPWAEDLCNAAAL
                     CNVRKVVRELQEEASPALLEPQASEKLLVVGAYYHLDSGRVEFLD"
     gene            8244..8486
                     /locus_tag="VFV30_07985"
     CDS             8244..8486
                     /locus_tag="VFV30_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848068.1"
                     /translation="MKEWTKKLGGGLAAVALMAGAVPGTVGAANAIEHGKGQMLDKGP
                     RWPHKSIHFVELALGVAVLAGLVLLFAGGTDDPASP"
     gene            8622..>8802
                     /locus_tag="VFV30_07990"
     CDS             8622..>8802
                     /locus_tag="VFV30_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apocarotenoid-15,15'-oxygenase"
                     /protein_id="HEX4848069.1"
                     /translation="MTVELVGTVRSTLKNTDHPYMQGAWRPTFNEWNALFESGDAEVI
                     GTIPDDIDGVYVRTGE"
BASE COUNT         1521 a         2869 c         2864 g         1548 t
ORIGIN      
        1 gtcgggcgtg cgggtctggg ggacgttcga tccccacagc atcaggaacc cggcattgta
       61 ccagtcagca ctttcgggaa cgtcggtctg ctcgccccag gtcatcgggc tggcgggtgg
      121 cagatcgcag taccagtcat agaagctcat gcaggtgccg ccgagcagcg agagataacg
      181 cgatcccgcc gcatagctga ccatcgacat tgccgggatc ggagagaacc cgatcacccg
      241 gtccggccca tactgcttgg cggtataggc attggccgcg gcgatgatct cgttcacctc
      301 gtcccaggtc gagcggacaa agccgccgtg gccgcggatc gcggtatagg acttgcgctt
      361 gaccggatcg gcctggatcg aggcccaggc ggcaaccgga ggcagatcct tgcgcgccgc
      421 ccgccacagc ttgaccagcc gcttgcggat cagcgggtat ttcagccgct gggccgaata
      481 gatgtaccag ctgtagctcg ccccgcgcgg gcagccgcgc ggttcgtgat tgggcagttc
      541 gggtcgggtg cgcggatagt cggtctgctg ggtttcccag gtgatgatcc cgcccttgac
      601 gtagatcttc cagctgcaag atccggtgca gttcaccccg tgggtgctgc gcacgatctt
      661 gtcgtgctgc cagcgcttgc ggtagctatc ctcccagtcg cgggcctcgg cggtggtgac
      721 cccgtggcct tcggaaaact gttccttctt ctggcggaag aacgtcaagc ggtcgagcag
      781 gtggctcatg cggtggctcc ttgggcaatg ggggctgtgg gggcgcggcc gcgctcgaca
      841 tcgtgcagga ggccgccctt gcgggtgtag atgagccagg tcagcgcggc gcagctggcg
      901 tagaacacga tgaagccata gagcgcggcc agcggcgttc ccgtggcgct gatcgacatc
      961 ccgaaggcac gggggatgaa gaacgcgccg taggcggcaa tggccgaagt aaagccgacg
     1021 atcgccgcgg attccttttc ggcctgacgg gtgcgatggg cggaatcgag ttccggcatc
     1081 agccgcggca cttcctggcg catgatgttc gggatcatct ggaacgtgga ggcattgccc
     1141 accccggtga cgaagaacat gaagataaag gcggccagga agccattgaa atccttgact
     1201 tcgaggaagt ggataacccc cagcgcgccg acgatcatgc cgatgaacac ccagaaggtg
     1261 actcgcgcgc cgccccagcg atccgcgatc cagccggtcg cggcgcggct gaccgcgccg
     1321 accagtgggc cgaggaacac gtatttcagc acgttgacgt cgggaaactg gcttttggcg
     1381 agcagcggca ggccggcgga aaagccgatg aacgagccga aagtgccggt gtaaagccag
     1441 cacatcagcc agttgtgctt gcgctggaag atcaccgcct gttcggcaaa gctggccttg
     1501 gcatcggcga tgtcatgcat cccgaaccag gcgagcaggg tgctggcgat gatgaatggc
     1561 acccagatga agccggcgtt ctgcagccac agttcgctgc catccttggc cacctgcgca
     1621 gtgccaccaa gggcaccaaa caccccggcc gttatcacca aaggtacggc aaactgcatc
     1681 accgagacac ccaggttgcc gagcccggca ttgagcgcca gcgcgttgcc cttttgcgcc
     1741 ttggggaaga agaagccgat gttggacatc gagctggcaa agttgccgcc gccaaagccg
     1801 cacagcaggg ccagcaccag gaagatgaaa tagggggtgt ccggatcctg caccgcatag
     1861 ccgatcccga aggccgggat cagcagcgag gcggtcgaca gcgtggtcca cagccgcccg
     1921 ccgaagatcg gcaccatgaa ggaatagaag atccggaagg tcgcgcccga caggccgggc
     1981 agtgcggcca gccagaacag ctggtccgtg gtgtagtcaa agccgatgag gggcagcttg
     2041 gccacgacca cggaccatac catccacact gcgaaggcaa gcagcagtgc ggggaccgaa
     2101 aggtagagat tgcggttggc gatgcgctgg ccggtttcct cccagaacgc cgaatcctcg
     2161 ggccgccagt cggtcaagac cttgcccttg accgcttgtt cgccgtgaga cgccgcatcg
     2221 tgcagcccgg ccatttccgg gaactgcggc agcgaacgct ggtcaatccc gctggccttc
     2281 tgctccatct ggcggatcgc caggtgcatc cagcccagcg cgaccgcaac cagcgcgaac
     2341 agcaccatga agcagctggt ccagatgccg gtcaggtcgc tcaccgcgcc gaacacgatc
     2401 ggcaggacaa acccgcccag cccgccaacc aggccgacca gcccgccgac cgaaccgacg
     2461 tggccggggt agtagaccgg gatgtgcttg tagaccgccg ccttgcccag cgacatgaag
     2521 aaaccgagca cgaacacggt gatgacaaag ggcaccaggc tcatcgaggt ggagaagcgg
     2581 atgtcgccct tgatcccgtg gatcgtgtag tccgtggccg ggtaggacag catgaacagg
     2641 cagagcattg acacgccaaa ggtggcgtac atgatccgcc gcgcaccata gcggtccgac
     2701 aggatcccgc cgtaggcccg gaacaccgaa gccgagaggc tgaaggttgc cgcgagcatc
     2761 ccggcgacct tgatgtccac gccgtaaacc ccgatcaggt acttgggcag ccacagcgcc
     2821 agggcaacga aagccccgaa gacgaagaag taatagagcg agaagcgcca gacctgctcg
     2881 ttcctgagcg gctccagctg ctccttcagg cccttgggct tttccccgct gatcttgcgc
     2941 gcggcaaggt cgggatcgtc cttggcgaac aggaagaaca gcaccgccgt gatcgccagc
     3001 accaccgccc agatctgggc caccgcctgc cagcccagcg ccaccatgac gaacggcgcg
     3061 acgaacttgg tcacggccgc cccgacattg cccataccga aaaagcccag cgccgagccc
     3121 tgcttttcct gcggatacca cttggagaca taggccacgc caaccgcgaa gccgccgccc
     3181 gccatgccca gccccagtgc ggccagcagc atcaggatat agctgtccgc ctggctgagc
     3241 agcacagtcg ataccgccga tgccagcatg gtcagcggaa agacaatccg cccgccgtac
     3301 tggtcggtcc agacccccag gaacaggcgg accagcgatc cggtcaggat cggagtgccg
     3361 accagcaagc cgaactgggt atcgttcagc cccagctcgg ccttgatctc gatcccgatg
     3421 atcgcgaaaa tcgtccagac tgcaaagcag atggtgaaag cgaaggtgct gagcccgagg
     3481 gctcggttct gttgtccgga gctgaccgtt tgcgacagtg gcatggcggc gtgttccctg
     3541 ttgtgggttg aatgccagcg aacctgtcca actctgccgc gaaaggcttg atccggatca
     3601 agaatggcct gttcggcccc gcaatcgtcg aaccgcctga tctgtagcgt cggatcgccc
     3661 gatccagatc aagcccgcga ttgatgcaca tcaatcgcac tgctaaggct ggcgcagttc
     3721 aaccaaggag ctgagctgcg tgcgccccgg cgaaagacct gaaatcgcga atctgcccct
     3781 gttccgggaa atgcaggacg gccaacgaga ccggatcttt tcgggatcgt tcctgcaggc
     3841 ctttcccccg cagctgaccc tgttcgaact gggccagcac ccggattttc tgcacgttct
     3901 ggtcgatggc ctggtcgagc tttacgccca cagcgccggg cgcgatacga ccatgcggat
     3961 cgtcgagccg gtcaccagct tcatcctggc cgccgtcatc accgatcagc cctacctgat
     4021 gtcggcccga acgatcgcgc catcccggat cctgctggtg ccggccgccc tggtccgcga
     4081 agtggtgaag gacgattgcg cgctgatgca ggcgaccatg cgcgaactgg cccaggccta
     4141 tcgcgacatg gttcgcgcgc tgaccgacat gaagctgcgc caatcggccg agcggctggg
     4201 caatctgatc ctgcagcaag agcgccgcca gggcggcagc ggcaatgttc agctgaaagc
     4261 cgaaaagcgt ctgctcgcct cgctgctggg aatgacgccg gaaaacctct cccgcgcgtt
     4321 cggggtgctg agcggacacg gcattgccgt atccggtgcg caagtgacga tcaccgaccg
     4381 cgccgcactc gaagcctttt cccgccccga tccggtgcgc gacgatctgg cctgatcaga
     4441 acttggcctc gatctggaac cacagcttgg tggtatcgct ggcaaaggca tcggcatcgt
     4501 aatgggccag cttggcggtg agggtaaacc tcttgcccag cggatagctg gccagcgcgt
     4561 tccattcctg gccataaggg attccgccgg tggtcgatcc gtaatcgtgc caggccgcgc
     4621 gcagcaccag gccggccagc ggccccttgc ccggcacctt gtatccggca tcgagataga
     4681 catcgcgcag gcccgaggct ggcgtagtca ggaacttgtc ggcccagccg ttgaaggcat
     4741 gcagtgttgc cagcggggtt tgcaggccga ccgtgccgtt gccttccagc cgttcgtagc
     4801 cggccttcag cgtcaccggc ccggccttca cccccggctc gaacagccag taatcaaggc
     4861 cgaagctttg cgggttggac ccgtagtcgt gctggcgagc atattcggcg gaatagagga
     4921 tggacgcttt cccccccact ggcagctcgc cagccatccg cacgcccagg gtcctattgc
     4981 tgctggccgg gcttgaggga acatccagcc agtagccgta ggcagagagc gtgccgacgt
     5041 tcttgatggt ataggcagcg cggaccccgt ggattttggt gtcgcgccag attccctgcg
     5101 gcgaatccgg gccgaacacc cgattgaccc gccaggcata gaagtaatcg acactggcgc
     5161 ccttcactgg ccgggccgtt acccgcactg tatcgagcgt ctggtcgttc tgacgccagc
     5221 cgaccgagcc gatccagcgc tggttgtcga aattgaccgc ctgacgcccg ccgaccattt
     5281 ccagctcggc catcggccgc cagcgcagat aagcctggtt aagcagcaca tcagacggat
     5341 cggccaccac cggataggcg gtcttgccgt tgaccgtgtc attgtaactg gtctcaccca
     5401 gcaccactat cgcttcgcct tccaccacgg cgctgaagct gtgccattcg gcggtcttga
     5461 tcccggcctt gacccgcagg gtagaggcag cggcctgttc ggccagacca tcctgatcga
     5521 ccagttccag ccggtagcga agatcgacat agggggtgac tggctgcgtc tcggcatggg
     5581 cggcagtgct ggccagcaag gcggcaagag tcagggcgat acgggatgag gcttgattca
     5641 tgtctgctat tctcgcctca attccgcttc tgtaattgac tttcatcaac gattattgag
     5701 gccgtccagc ccaaagggat gttgatggaa atcgacactt tcactctcgc ccgcggtctt
     5761 cacgttgtcg cgatcgtcgg ctggatcggc ggtgtctggt ttgtcacgct ggtggtcatg
     5821 cccgcgattg tccgcagcga gccgccagcc aacaggcttt cggcctttca ccggatcgag
     5881 caggggtttg ccccgcaagc gaagttctgg gttctgctgg ccggggccag cggcttctgg
     5941 atgactcacc aggccgacct gtggggccgg tttgccgatc ccgccttctg gtggatgcac
     6001 gccatgctgg ggctctggac gatctttgcg gtgatgctgt tcgtggccga gccgctgttc
     6061 ctgcaccgcc gcatggcccg gtcggccacg cccgaggccg actgggcccg aatggtgacc
     6121 atgcaccggc tgctttccgc gattgccctc gtcaccgtga tcggggccac gggcggcgcg
     6181 catgggctgt tctgatgacc agccccgccc ccctgctctt gtccgcgccg caccgcctgg
     6241 ccttcctcac cggcagcctg ggcttgaccg ccatggcttt gtggtggccg ctgcggctgg
     6301 ccgcgctcca ccttggctgg tcggagccgc caactggggg tctgcccgcc gctctgctcc
     6361 atggcccggt gctgttgctg catgtctatc cggctttcat cttcggcttt ctgctgaccg
     6421 ttttcccacg ctggatgggc cagccggacc tcgacgcgcg ccggttcgga ccgatctcgg
     6481 ccggactggc cgcaggcatg gccgcaacaa cgatcggcct gtggactggc agcgacgcgg
     6541 ccgtgcggac cggatttacg atcgtcgcgg ccgcctgggc gctcggcctg ttcgtgctgg
     6601 ctgtcgtggt tcgggcgaat gggcggtcgg gcaagccgcc ctgctggcac gccgtgtccg
     6661 ccctggctgc ccttggcctg ggcctgggcg cgcttggcct ggtggccgcg ttcctttttg
     6721 gtggcgaccc ccggctgctg cgctggggca atgtgctggg gctgaacctg ttcatcctgc
     6781 cggtgttcct gaccgtagcc caccggatgg tgccgttctt tgccggcaat gtcgtgacgg
     6841 gctatcggcg ctggcggccg gactggctgc tggccgcgct gtggacgctg ctggtgctgc
     6901 ggatcgcggg cgaactggcg ctggttccgg ccttgtcagg actggctgcg acgggactgg
     6961 ccgcctgcac cggcctgatg gcgtggaaat ggtggccgcg cggccccgcg ccgggtctga
     7021 tgcaggtgct gatctggggc tttgcctggg ccccgctcgg ctttgccctg tccgcgctgg
     7081 ccagccttgg cctgccgctc ggcctggcac ccacccatgc gctggctctt ggttttgcgg
     7141 ccagtctgct ggtggcgatg gtgacccgcg tcacgcatgg ccattcgggc cggccgctgg
     7201 cgatggtgcc gctggcctgg ctggcctttg ccgccgtcca gctggccgcc gggctgcgga
     7261 tcgtggcggc gataaagggc gaaccgggcg tgctgctggt aagcggcgcg ctggcccttg
     7321 ctgccggcct gctgccctgg ctgatccgca acgcgctgat ctatctgcgc ccgcgcctcg
     7381 atggccgcga tggatagtcc gccgcagatc accccgcgcg cggcgctgga actgctcaag
     7441 gaaggcaacc gccggttcct gtccgacgcg ccctacaacc cgccgatgga ccggctccgc
     7501 cggctccacc tcgccgccgc gcagcgtccc ttcgcggcct atctcagctg ctcggattcg
     7561 cgcgtcccgc ccgaactgct gttcgaacgg gggttgggcg agctgttcat cgtccgcaat
     7621 gccggcaaca cgctttgcgc cacggcgctg ggcagcctag aatttgctgt cacccagttg
     7681 caggtgccgc tgatcgtggt gatgggccac gaagcctgcg gcgcgatccg ggcggcggtt
     7741 tccgtcgccc gcggagagac ggcctatacc ggcaacgtct ccaaggttct gcaatccctg
     7801 ctccccgccg tcctcgaagc cgacccgtgg gccgaagacc tctgcaacgc cgccgcgctg
     7861 tgcaacgtgc gcaaggtcgt gagagagctg caggaggaag cctcccccgc cttgctcgaa
     7921 ccacaggcca gcgaaaaact gctggtggtc ggtgcctact accacctcga ttccgggcgg
     7981 gtcgaattcc tcgattgagc agtctggaac gttgatcgag atctggtcca gggagcgttt
     8041 gcaggagacg tttcgccacc agactgacag caccgccacc ttccgttgaa tcgaaccgca
     8101 cttcgccgca gcctcccatg gcgcgcggcg gcagcgattt tccgcgccga ttgaaccgtt
     8161 tgcccgccaa ccgcgactgt ccctgccgac cggcatttcg ccgcgccggc atccttgtgc
     8221 acagagggaa aggacattcg cagatgaagg aatggacgaa aaagctggga ggcgggctgg
     8281 ctgccgtggc cttgatggcc ggggcggtac ccggcacggt cggggccgcc aacgcgatcg
     8341 aacacggcaa gggccagatg ctcgacaagg ggccgcgctg gccgcacaag tcgatccact
     8401 tcgtcgagct tgcgctgggc gtggccgtgc tggcgggcct ggtcctgctg tttgccggcg
     8461 gcacggatga tccggccagc ccctgacgga ccggttgggg ggcggtctgg cccggccggg
     8521 ccgcccttcg gcatccaccc tcgatccgca ttgtcgcgcg ccgcgatcca gaataggctg
     8581 gcttgaacac aagccgcgcc tcggcggcag ggagtggatc tatgacggtg gaactggtcg
     8641 gaaccgtgcg ctcgacgctg aagaacacgg accatcccta tatgcagggg gcctggcggc
     8701 cgaccttcaa cgaatggaac gcgctgttcg aaagcggcga tgccgaagtg atcggcacga
     8761 tcccggacga tatcgacggg gtctatgtcc gcaccggcga gg
//
LOCUS       DASUDV010000364         5204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_785543,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000364 DASUDV010000000
VERSION     DASUDV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785543"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /gene="dinB"
                     /locus_tag="VFV30_07995"
     CDS             complement(<1..1119)
                     /gene="dinB"
                     /locus_tag="VFV30_07995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX4848070.1"
                     /translation="MAADDDEQGEDAGGASGLRKIIHVDMDAFYASVEQRDEPALRGK
                     PVAVGGEGPRGVLTTCSYEARKFGCRSAMPSVTARRLCPQLIFRPPRFAVYTAISHQI
                     REIFLDYTPLVEPLSLDEAFLDVTEDSKGIGSATRIAELIRARIKAETGLTASAGVSY
                     NKFLAKLASDQNKPDGLCVIRPGEGADFVAGLPVRRFFGIGPKGTEKMAALGIETGAD
                     LRARDIHFLRQHFGSQADYLYRAARGIDLRRVKANRARKSLGAERTLDKDISSGSALR
                     GVLEQIIALTWTRIEKAEARGRTVTLKLKHKDFTGLTRARSLARPVASREEFAAIGHA
                     LLDEVLPLAQPVRLVGLTLSALDEDESPAPPVAGTVQGQ"
     gene            1196..2443
                     /locus_tag="VFV30_08000"
     CDS             1196..2443
                     /locus_tag="VFV30_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX4848071.1"
                     /translation="MSRTGQTSPRPALGEREFIVLMASLMALQALSIDSMLPALGTIA
                     SDLGSSDPNHRQLVIGVYLFGSAIGALLPGALADRFGRRPVLFGALAVYTLLALACAM
                     VQDFTTLLVLRGIQAVGSAGLTVMPSAIIRDCHEGDRMARMLSTTALVFMIVPILAPS
                     FGQGVLQFAGWRWIFGLTAVLSVAVSAWVWLRLPETLNPANRQEIRPRRIALNMGTAL
                     TNRASIGYVLASGLVWAGLFGFLNSSQQLVAEHFGAGSSFPLYFAAMAGCMAMANLLN
                     SRIVERFGARRVSHTALLAFIALAALQVSRAYAGDETLTQFAVMMAAQMCLCALMGAN
                     FTSIALQPFGHIAGAAASVQMFTRSVLSSLLGGAIGQAFDGSARPLATALLGGGLLAL
                     ALVFYSEKGRLFRRLNPPGRQVA"
     gene            2440..3897
                     /locus_tag="VFV30_08005"
     CDS             2440..3897
                     /locus_tag="VFV30_08005"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX4848072.1"
                     /translation="MSEIYDLAVIGGGVNGAGIARDAAGRGAKVLLLEGGDLAQGTSS
                     ASTKLIHGGLRYLEHYEFALVRESLTEREALWRIAPHIIHPMRFVLPYVDGLRPRWLL
                     RLGLFLYDHIGGRKRLPATRTVDLRRHAAGGPLKGGFSTGFEYSDGWVDDARLVVLNA
                     RDTAERGGAVRTRTRVTGARREDGLWRIEAGNETFQARALVNAAGPRVLEVEKLAGDA
                     PEQAMRLVRGSHIVVPRLFDHPYAYFFQLPDGRIFFAIPYERDFTLIGTTDVDHDRSL
                     DKVEASAEEIAYLCEGASRYFAKTITPADVVWTYSGVRPLVSDNSGKPEAATRGYRLD
                     LSDAGEGAPLLSVYGGKITTYRHLAEEAVDLLAARLPAIAGKGWTRNEPLPGGDFAID
                     GLDALIAAYARDYPFLAPGTADRVARAYGTRARLWLDKDQGRDFGGGLSQAEVDYLVA
                     EEWAVSAEDVLWRRTKLGLRLSKEQAAALADYLGG"
     gene            3934..4341
                     /locus_tag="VFV30_08010"
     CDS             3934..4341
                     /locus_tag="VFV30_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848073.1"
                     /translation="MAPMLRAPFALLCVLALSGCSYIYGVEAKMSGGRVVFEADPQWG
                     KDCVRKIVVRAGDTRGSAAVWDQSVSYEDDCANTFPVRYGDAFKGRSERAAKPQPLRT
                     GVVYTVETTTGATGYGCGRFRLLDDRRVEDLGC"
     gene            complement(4514..>5204)
                     /locus_tag="VFV30_08015"
     CDS             complement(4514..>5204)
                     /locus_tag="VFV30_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4848074.1"
                     /translation="REAKQVYSMRKLLDAAFDKGSVFEMGRQWGRAAITAFARLDGWP
                     VAVVASDPSFLGGSWEAKTSEKVERFIKLADQFRLPVVHLVDNPGFMIGLAAETSGTI
                     RYGVNAMNAIYKASVPLCCVVIRRAYGIAGSAMSNAEAFQYRFAWPSGDWGSLPIEGG
                     IEVAYKSEIEAADDPQAHLEAIRERLNRVRSPFRTAEKFGIEDIIDPRETRPLLCEFA
                     DLAWRALGAKS"
BASE COUNT          740 a         1766 c         1819 g          879 t
ORIGIN      
        1 ctggccctgg accgtgccgg cgacgggcgg ggcaggggac tcgtcctcgt ccagcgccga
       61 gagcgtcagg ccgaccagcc ggaccggctg ggccagcggc aggacctcgt ccagcagggc
      121 gtggccgatc gcggcgaact cctcgcggct ggccaccggg cgggccaggc tgcgcgcgcg
      181 ggtcagcccg gtgaagtcct tgtgcttcag tttcagcgtc acggtccgcc cgcgggcctc
      241 ggccttttcg atccgggtcc aggtcagcgc gatgatctgt tccagcaccc cgcgcagcgc
      301 cgagccggag gaaatgtcct tgtcgagcgt gcgttcggcc cccagcgact tgcgcgcccg
      361 gttggccttg acccggcgca ggtcgatgcc ccgggccgcg cggtagaggt agtcggcctg
      421 gctgccgaag tgctggcgca ggaaatggat gtcgcgcgcg cgcaggtccg cgccggtctc
      481 gatccccagg gcggccatct tctcggtccc cttgggcccg atcccgaaga accgccgcac
      541 cggcaggccg gcgacaaagt cggcgccctc gccgggccgg atcacgcaaa ggccgtcggg
      601 cttgttctgg tcgctcgcca gcttggccag gaacttgttg taagagacgc cggcgctggc
      661 ggtcagcccg gtttcggcct tgatccgggc ccggatcagc tcggcaatgc gggtggccga
      721 tccgatcccc ttgctgtcct cggtcacgtc aaggaacgcc tcgtccaggc tcagcggctc
      781 gaccagcggg gtgtaatcca ggaagatctc gcggatctgg tgcgaaatcg cggtgtagac
      841 cgcaaagcgc ggcgggcgga agatcagctg cgggcagagc cggcgtgcgg tgaccgaggg
      901 catggcgctg cggcagccga acttccgcgc ctcgtaggaa caggtggtca ggaccccgcg
      961 cggcccctcg ccgccgaccg ccaccggctt gccccgcagg gccggctcgt cgcgctgttc
     1021 gacgctggca tagaaggcat ccatgtcgac atggatgatc ttgcgcaggc cgcttgcccc
     1081 gccggcgtct tcgccctgtt cgtcgtcatc cgctgccatg cgcatacgat agcgcgaata
     1141 gccgcgtgac gaaaatgcac tggccagcac ggcccgcctc ggccatgggc cctgcatgtc
     1201 ccggaccggc cagaccagcc cgcgccctgc cctgggcgag cgggaattca tcgtcctgat
     1261 ggcgtcgctg atggcgctgc aggcgctcag catcgattcg atgctgccgg cgctgggcac
     1321 gatcgcgtcc gatctggggt cgagcgatcc caaccaccgc cagctggtga tcggggtcta
     1381 cctgttcgga tcggcgatcg gggcgctgct gcccggggcc ctggccgacc ggttcggccg
     1441 ccgcccggtg ctgttcggcg ccctggcggt ctataccctg ctggccctgg cctgcgcgat
     1501 ggtccaggat ttcaccaccc tgctggtgct gcgcgggatc caggcggtcg gcagcgccgg
     1561 gctgaccgtg atgcccagcg cgatcatccg cgattgccac gagggcgacc ggatggcgcg
     1621 gatgctctcc accacggcgc tggtcttcat gatcgtgccg atcctggccc ccagtttcgg
     1681 ccagggcgtg ctgcagtttg cgggatggcg gtggatcttc gggctgaccg cggtgctttc
     1741 ggtcgcggtt tcggcctggg tctggctgcg cctgcccgaa acgctcaatc cggccaaccg
     1801 gcaggaaatc cgcccgcgcc ggatcgcgct caacatgggc acggcgctca ccaaccgggc
     1861 ctcgatcggt tacgtgctcg cctccgggct ggtctgggcc gggctgttcg ggttcctcaa
     1921 cagctcgcag cagctggtgg ccgagcattt cggcgccgga tcgtccttcc cgctctattt
     1981 cgcggccatg gccgggtgca tggcgatggc caacctgctc aattcgcgga tcgtggaacg
     2041 gttcggcgcg cgccgggtct cgcacaccgc gctgctggcc ttcattgccc tggccgcctt
     2101 gcaggtcagc cgcgcctatg ccggggacga gacgctgacc cagttcgcgg tgatgatggc
     2161 ggcgcagatg tgcctgtgcg cgctgatggg ggccaatttc acctcgatcg cgctgcagcc
     2221 ctttggccat atcgccgggg cggcggcctc ggtgcagatg ttcacccgct cggtcctttc
     2281 ctcgctgctc ggcggggcga ttggccaggc ctttgacggg tccgcacgcc cgctcgccac
     2341 ggccctgctg ggcggcggct tgctcgcgct ggccctggtg ttttacagcg agaaggggcg
     2401 gctgttccgc cgtctcaatc cccccggcag gcaagtggca tgagcgagat ttacgacctg
     2461 gcagttatcg gcggcggcgt gaacggcgcg ggtatcgcgc gcgacgctgc cggacgcggg
     2521 gccaaggtgc tgctgctgga aggcggcgac ctggcccagg gcaccagctc ggcctcgacc
     2581 aagctgatcc acggtgggct gcgctatctg gagcattacg agttcgcgct ggtccgcgaa
     2641 tcgctgaccg agcgcgaagc gctgtggcga atcgccccgc acatcatcca cccgatgcgc
     2701 ttcgtcctgc cctatgtgga cggactgcgg ccgcgctggc tgctgcgcct cggcctgttc
     2761 ctttatgacc acatcggcgg gcgcaagcgc ctgcccgcca cccgcacggt cgatctgcgc
     2821 cgccatgccg cgggcgggcc gctgaagggc ggcttctcga ccgggttcga atattcggac
     2881 ggctgggtgg atgacgcccg gctggtcgtg ctcaacgcgc gcgacacggc cgaacgcggc
     2941 ggcgcggtgc ggacccgcac ccgggtgacc ggggcgcggc gcgaggacgg gctgtggcgg
     3001 atcgaggccg ggaacgagac ctttcaggcc cgcgccctgg tcaacgccgc cgggccgcgc
     3061 gtgctggaag tggaaaagct ggccggtgac gcgcccgagc aggcgatgcg gctggtccgc
     3121 ggcagccaca tcgtcgtgcc gcggctgttc gaccatccct acgcctattt cttccagctg
     3181 cccgacggcc ggatcttctt cgcgatcccc tatgaacggg acttcaccct gatcggcacc
     3241 accgatgtcg accatgaccg ctccttggac aaggtcgagg ccagcgccga ggaaatcgcc
     3301 tacctgtgcg aaggggccag ccgctatttc gccaagacca tcaccccggc cgacgtggtc
     3361 tggacctatt ccggggtgcg cccgctggtc agcgacaatt cgggcaagcc cgaagcggcc
     3421 acccgcggct accggctcga cctgtcggat gcgggcgagg gcgcgccgct gctctcggtc
     3481 tatggcggca agatcacgac ctaccgccac ctggccgaag aggccgtgga cctgctcgcc
     3541 gcgcgtctgc cggcgatcgc gggcaagggc tggacccgca acgagccgct gcccggcggg
     3601 gacttcgcga tcgacgggct cgatgcgctg atcgccgcct atgcccgcga ctatccgttc
     3661 ctggcgccgg gcaccgccga ccgcgtcgcc cgcgcctatg gcacccgggc ccggctctgg
     3721 ctggacaagg accagggccg cgatttcggc ggcgggctca gccaggccga ggtcgattac
     3781 ctggtggccg aggaatgggc ggtcagcgcc gaggacgtgc tgtggcgccg caccaagctg
     3841 ggcctgcggc tgagcaagga gcaggcggcg gcgttggcgg actatctggg cgggtaggtc
     3901 ccctccttgc gggaaaaggc agatggccgc atcatggccc ccatgctgag agcgcccttc
     3961 gcactgcttt gcgtgctcgc cctttcggga tgctcgtaca tttacggcgt agaggcaaag
     4021 atgtccggcg gacgggtagt gttcgaggcc gatccgcaat ggggcaagga ttgcgttcgc
     4081 aagatcgtgg tcagagcggg cgacacgaga ggctcagccg cagtttggga ccagtctgtt
     4141 tcgtacgagg acgactgcgc gaacacgttt ccggtccgct atggcgacgc gttcaaggga
     4201 cgcagcgaaa gggcggccaa gccgcaaccg cttcgcaccg gcgttgtcta taccgttgaa
     4261 acgacgaccg gcgcgaccgg ctatggctgt ggccgcttcc gccttttgga cgatcggcgc
     4321 gtcgaggatc tgggctgctg acggggccta tccggatcag aggaacctat cggatcctcc
     4381 ctgtcgcgaa gcgatgggga ggtggcagcg cgcagcgctg acggaggggc tatggcgcaa
     4441 gggtggaagg ccccaccacc atcgcctccg gcgacggtcc ccctccccat gagctgcgct
     4501 cacagggagg atcttagctc ttcgccccca gcgcccgcca cgccagatcg gcgaattcgc
     4561 agagcagcgg ccttgtctcg cgcgggtcga tgatgtcctc gatcccgaac ttctcggcgg
     4621 tgcggaacgg gctgcgcacg cggttgaggc gttcgcggat cgcttcgaga tgggcctgcg
     4681 ggtcgtccgc ggcttcgatc tcgctcttgt aggccacttc gatcccgcct tcgatcggca
     4741 ggctgcccca gtcgccgctg ggccaggcga agcggtattg aaaggcttcg gcgttgctca
     4801 tggcgctgcc ggcaatgcca taggcccggc ggatcaccac gcagcacagc ggcacgctgg
     4861 ccttgtagat cgcgttcatg gcgttcacgc cgtaacggat cgtgccgctg gtttcggcgg
     4921 cgaggccgat catgaagccg gggttgtcga ccaggtggac caccggcagg cggaactggt
     4981 cggcaagctt gatgaaccgt tccaccttct cgctggtctt ggcttcccac gatccgccga
     5041 ggaagctggg atcgctggcg acgaccgcga ccggccagcc atccagccgg gcaaaggcgg
     5101 tgattgcggc gcggccccac tggcggccca tttcaaagac cgagcccttg tcgaaggcgg
     5161 catccagcag cttgcgcatc gaatagacct gcttggcctc acgc
//
LOCUS       DASUDV010000365         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1094909,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000365 DASUDV010000000
VERSION     DASUDV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094909"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFV30_08020"
     CDS             complement(<1..352)
                     /locus_tag="VFV30_08020"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX4848075.1"
                     /translation="MANVTVIGAQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLV
                     VGDKVYKLSLLPSGIVTGTLSIIGNGVVLDPWHFRDEVAKLKGQGVDVNPENLQVAEN
                     APLILPFHRDLDALR"
     gene            626..1543
                     /locus_tag="VFV30_08025"
     CDS             626..1543
                     /locus_tag="VFV30_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848076.1"
                     /translation="MRRSIAFGLVATALLATGLAQAAPGERLRERVAERTSGEPAQRA
                     ARPQTIAYGSDPLQQLDVWKADKAGGKAPLVMYVHGGGWKRGSKETAASRNAPAHFTR
                     EGYVYASIDYRLVPKNTVEDQARDVAQALKALLEKADALGIDRSRVVIMGHSAGAHLV
                     ALVGTDEQYLRSAGLSFADIDGVIPNDGAAYDVPAQMRQAGRLMQDTYDQAFGTDPAR
                     QRALSPTLQAASPNAPAFLLIHVQRKDGVAQARELEAALRRAGTAVERREFAGQGLRG
                     HMEINRNLGDPDYPATPVVDSWLKRIFAR"
     gene            complement(1540..2667)
                     /locus_tag="VFV30_08030"
     CDS             complement(1540..2667)
                     /locus_tag="VFV30_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4848077.1"
                     /translation="MQDTERDLLPEGLEDRLPQDAAAAARVTRAVLDVLDAHGYDRVQ
                     PPSIEFEMSMASRMAGVQPRRMFRFVDPASLRTLALRSDITVQVGRIAATALASAPRP
                     LRLCYAGQVVTIKGDGLDPSRERLQLGAELIGSDSVAAAAEIVATAIEALQAAGARGI
                     SVDFTLPDLVDTLAAKALPLAPDRIAAVRQELDAKNAGGLVAAGGEAYLPLLTAIGPF
                     DAAIERLAAIDAANQSGSGALSSRIAGLREIAARVSGMARLTLDPSERHGFEYQSWFG
                     FMIYAEGVAGALGRGGSYRILGIDEPAIGFSLFPDHLIDLQAGSELRRDTLFLPLGHD
                     PGHAARLRAIGWRTIAALCADCDPVALGCTHRLEGEEAVRL"
     gene            complement(2772..>4022)
                     /gene="serA"
                     /locus_tag="VFV30_08035"
     CDS             complement(2772..>4022)
                     /gene="serA"
                     /locus_tag="VFV30_08035"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX4848078.1"
                     /translation="FALARQIPAADAKTQAGLWPKNDFMGVEVTGKTLGLIGAGNIGS
                     IVASRALGLRMKVVAFDPFLTPERALEMGVEKAELDVLLARADFITLHTPLTEQTRNI
                     LSRENLGKTKKGVRIINCARGGLIDEEALKDGLDCGHIAGAALDVFQTEPATASPLFG
                     TPNFICTPHLGASTTEAQVNVALQVAEQMADYLVYGGVTNALNMPSLSAEEAPKLKPY
                     MALAEKLGSLVGQLAHGNLTEISIEREGAAAALNGKPIEGAVLAGFMKQYSDSVNMVN
                     APFLAKERGLDVRSVRHEREGVYHTLLKVTVATSQGPRSVAGTLFGNAAPRLVEIFGI
                     NIEADLDGHMLYIVNEDAPGFIGRIGSLLGEKGINIGTFHLGRREAGGEAVLLLSVDS
                     PVSKDVIEAAGKLPGVKRVMALAF"
BASE COUNT          675 a         1363 c         1343 g          641 t
ORIGIN      
        1 cgcgcagcgc atcgagatcg cggtggaacg gcaggatcag cggggcgttt tcggcgacct
       61 ggaggttttc ggggttcaca tcgaccccct gccccttgag cttggcaact tcatcgcgga
      121 agtgccaggg atccagcacc acaccgttac cgatgatcga cagcgtgccg gtgacgatcc
      181 ccgaaggaag caggctcagc ttgtagacct tgtcgcccac caccagagtg tgcccggcgt
      241 tgtgcccgcc ctgaaagcgc accacggcat cggcccggct ggccagccag tcgacgatct
      301 tgcccttgcc ctcatcgccc cactgggcgc cgataacggt gacatttgcc atggatgcaa
      361 tcctatccct gccgggacaa tccacagggt ccttcgacag gactcgaccc agggactttt
      421 ccaaagggaa ccaaggctgg ctccggatgc gggtgcgccc ctagtcgccc tgggggcgga
      481 gagtcaagcg gcacggccgc accgccaaca ggcaagcacg cgccagtgcg acaagccgcg
      541 acactttgcg accgatccgt tcaccggcag gcaagcattt gcaccgcacg attgcgggcg
      601 ataccctctg gcaaggattg aaacgatgcg cagatcgatc gccttcgggc ttgtcgcaac
      661 agcacttctg gcaacgggcc tggcccaggc cgcgccgggt gagcggctgc gcgaaagggt
      721 tgcggagcgg acgagcggcg agccagccca gcgggcagcg cgcccgcaga ccatcgccta
      781 tggttccgat ccgttgcagc agcttgatgt gtggaaggca gacaaggccg ggggcaaggc
      841 gccgctggtg atgtacgttc atggcggcgg gtggaaacgc ggcagcaagg aaaccgccgc
      901 cagccggaac gcgcccgcgc acttcacccg ggaaggctat gtctacgcct cgatcgacta
      961 tcgcctggtg cccaagaaca cggtggaaga tcaggcccgc gatgttgccc aggcgctcaa
     1021 ggcgctgctc gagaaggccg atgcgctggg gatcgaccgc agccgcgtgg tgatcatggg
     1081 ccacagcgcg ggcgcgcacc tggtggcgct ggtcggcact gacgaacagt acctgcgttc
     1141 ggccgggctc agctttgccg atatcgacgg ggtgattccc aacgacggcg cggcctatga
     1201 cgtgccggcc cagatgcgcc aggccgggcg gctgatgcag gatacctacg atcaggcctt
     1261 tggcaccgat cccgcccgcc agcgcgcgct ttcgccaacc ttgcaggcgg ccagccccaa
     1321 tgcgcccgcg ttcctgctga tccatgtcca gcgcaaggac ggcgtggccc aggccaggga
     1381 gcttgaggct gcgctgcgcc gcgccggcac ggcggtggag cgccgcgagt tcgccggcca
     1441 gggcctgcgc ggccacatgg aaatcaaccg caacctgggc gatccggact atccggcaac
     1501 cccggtggtg gatagctggc tcaagcggat ttttgcccgc taaagcctga ccgcctcttc
     1561 gccctcaagc cggtgggtgc agcccagcgc caccggatcg caatcggcac acagtgcggc
     1621 aatcgtgcgc cagccgatcg cgcgtagccg cgcggcgtgg ccgggatcat ggcccagtgg
     1681 caggaacagg gtatcgcggc gcagttcgct gccggcctgg agatcgatca gatggtcggg
     1741 gaacagcgaa aagccgatcg ccggttcgtc gatcccgagg atccggtagg aaccgccccg
     1801 acccagcgcg ccggccacgc cttcggcata gatcatgaac ccgaaccacg actggtattc
     1861 gaagccgtgc cgctcgctcg gatcgagcgt gagtcgcgcc atgcccgaaa cccgcgcggc
     1921 gatttcgcgc aggcccgcga tccggcttga cagcgcaccg ctcccggatt gattggcggc
     1981 atcgatcgcc gccagccgtt cgatcgccgc atcgaacggg ccgatcgcgg tgagcagcgg
     2041 cagataggcc tcgccgcccg cagccaccag cccgccggcg ttcttggcat caagttcctg
     2101 gcgcaccgcc gcaatcctgt cgggtgccag cggcagggcc ttggcagcca aagtatcgac
     2161 caggtcgggc agcgtgaaat cgaccgatat cccgcgggct ccggcggcct gcagcgcctc
     2221 gatcgcggtg gcgacaatct cggccgcagc ggccacgcta tcgctgccga tcagttcggc
     2281 gcccagctgc aggcgctcgc gcgagggatc gagcccatca cccttgatcg tcaccacctg
     2341 cccggcatag cacagccgca aggggcgcgg cgccgaggcc agcgcggtcg cggcaatccg
     2401 cccgacctga acggtgatgt cgctgcgcag cgccagggtg cgcaagctgg ccggatcgac
     2461 gaagcggaac atccggcgcg gctggacccc ggccatgcgg ctggccatcg acatctcgaa
     2521 ctcgatcgag ggcggctgga cccggtcata gccatgggca tcgagcacat cgagcaccgc
     2581 gcgggtcacc cgcgcggcgg cggcggcatc ctggggcagc cggtcttcaa gcccttcggg
     2641 caacagatcg cgttcggtat cttgcatagt catctcggtt gacagtcatc ccggcgaagg
     2701 ccgggatcgc tggccaggtg gttcgaccta gcggccagcg atcccgggtc aagcccggga
     2761 tgacgtccgt ttcaaaacgc cagtgccatg acgcgcttga cgcccggcag tttgccggcc
     2821 gcctcgatca cgtccttcga caccgggctg tcgacgctga gcagcagcac cgcctcgccc
     2881 cccgcctcgc gccgaccgag gtggaaggtg ccgatgttga tgcccttttc gcccaggagc
     2941 gagccgatcc ggccgatgaa gccgggcgcg tcctcgttga cgatgtagag catgtggcca
     3001 tcgagatcgg cctcgatgtt gatcccgaag atctcgacca gccgcggcgc ggcgttgccg
     3061 aacagcgtgc ccgccaccga acgcggcccc tggctggtcg ccacggtaac cttgagcagg
     3121 gtgtgataga ccccttcgcg ctcatgccgg accgaacgca cgtcgagccc gcgttccttg
     3181 gcgaggaacg gcgcgttgac catgttcacg ctgtccgaat actgcttcat gaagccggcc
     3241 agcaccgcgc cttcgatcgg cttgccgttg agcgcggcgg ccgcgccttc acgttcgatg
     3301 ctgatttcgg tcaggttgcc gtgcgccagc tgcccgacca gcgagccgag cttttcggcc
     3361 agcgccatgt agggcttgag cttgggcgct tcctcggcgc tgagcgaggg catgttgagc
     3421 gcgttggtca ccccgccgta gaccagatag tcggccatct gctcggccac ctgcagcgcg
     3481 acattgacct gggcttcggt ggtcgaggcg ccgaggtgcg gggtgcagat gaaattgggc
     3541 gtgccgaaca acggcgatgc ggtggccggt tcggtctgga acacgtcgag cgcggccccg
     3601 gcgatgtgcc cgcaatcgag cccgtccttg agcgcttcct cgtcgatcag cccgccgcgc
     3661 gcgcagttga tgatccgcac gcccttcttg gtcttgccca ggttttcgcg gctcaggatg
     3721 ttgcgggtct gctcggtcag gggcgtgtgg agcgtgatga aatcggcccg ggccagcaga
     3781 acgtcgagtt cggccttctc gacccccatt tccagtgcac gttcgggggt gaggaacgga
     3841 tcgaaggcga cgaccttcat cctgaggccc agcgcccggc tggcgacgat cgagccgatg
     3901 ttgcccgcgc cgatcaggcc cagcgtcttg ccggtcacct cgacccccat gaaatcgttc
     3961 ttgggccaca ggcccgcctg ggtcttggca tcggccgccg ggatctgacg ggccagcgcg
     4021 aa
//
LOCUS       DASUDV010000366         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1448037,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000366 DASUDV010000000
VERSION     DASUDV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448037"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1438
                     /gene="murD"
                     /locus_tag="VFV30_08040"
     CDS             89..1438
                     /gene="murD"
                     /locus_tag="VFV30_08040"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX4848079.1"
                     /translation="MITSSAFAGKRYAVLGLARSGMTAVESLLAGGAQVTAWDSREEP
                     RRKLEGRCELADPVGIDLTGFAGVVVSPGVPLNTHPIAKVAEAAGVPVIGDIELFAIA
                     RPELPAHRVVGITGTNGKSTCTALVHHLLQSAALPTRMGGNIGLPILGANPLDAGGVY
                     VLELSSYQIDLTHSLQCDVAALLNITPDHLDRYDGFAGYAAAKARLFAMQRADQFAIF
                     GMGDAKTQAVAARELQTRAPGKVRSVDGRDLAALQPDWPSLQGPHNLQNAAVAVAIAE
                     ALGLTAMQWRPALASFRGLPHRMERVAEANGVMYINDSKATNPASTAPALAAFPPNPN
                     RRIHWILGGLPKGDDLNECAPHFGNIAAAYTIGDAGPRFAELLSPHMPVTRSEMMCEA
                     IQQAMAAAQPGDVVLLSPACASFDQFRDYEARGDAFRQIVQTLLTPEPAGATGGGRG"
     gene            1438..2658
                     /locus_tag="VFV30_08045"
     CDS             1438..2658
                     /locus_tag="VFV30_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX4848080.1"
                     /translation="MASNPPLVPRGGKVGVEGGAVRAKRNRRGELAIWWREIDRTMLA
                     LVLTLMAMGAAAVAASSPASARRLSTASVRLDDLHFFYLHIRYQILGLIAMFGASMLP
                     RDMARRAGIVLCFAMLVGLMLVPVIGFSVNGARRWLNLGMSLQPSEFLKPAFAIGMAW
                     ILSWRARDPNLPVIEMSTGLMILIAGLLMMQPDFGSAILFIGVWFVMVLMSGISLKRI
                     GLAGGAGVGFIVLAYMFYENARNRIDSFLGGGTAFDQVDLAQRTLLAGGWTGSGLWLG
                     TRKLGLPEAHTDYIFSVIGEEFGLIACALIVMIYLALVTRVLIRLLDEEDLFTTLAAA
                     GLIAQIGGQAFINVLVNLQLFPSKGMTLPLISYGGSSTIALCLGVGFLLSITRRNPFI
                     KREPFNLASLGIKL"
     gene            2655..>2912
                     /locus_tag="VFV30_08050"
     CDS             2655..>2912
                     /locus_tag="VFV30_08050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4848081.1"
                     /translation="MSGATRHYVLAAGGTGGHLIPAFALAVELERRGHHVALITDDRG
                     SRLPGKPHSLVAHVLPAGRIGKNPLKWPKGIEAILEGRRMAL"
BASE COUNT          424 a         1020 c          976 g          492 t
ORIGIN      
        1 tgggctggaa ggaatcgacc gtcgtgatcc gcttctggat cgtctcgatc atcctggccc
       61 tgatcggact gtcgacgctg aaactgcgat gatcacctcc tccgcctttg ccggcaagcg
      121 ctatgccgtc ctcgggcttg cccgctcggg gatgacggct gtcgaaagcc tgctggccgg
      181 cggagcgcag gtgaccgcct gggacagccg cgaggaaccg cgccgcaaac tggaagggcg
      241 ctgcgagctg gccgatccgg tggggatcga cctgaccggc tttgccggcg tcgtcgtctc
      301 gcccggggtg ccgctcaaca cccatccgat cgccaaggtg gccgaggccg ccggggtgcc
      361 ggtgatcggc gacatcgagc tgttcgcgat tgcccggccc gaactgcccg cgcaccgcgt
      421 ggtcggcatc accgggacca acggcaagtc gacctgcacc gcgctggtcc accacctcct
      481 gcaatcggcc gcgctgccaa cccggatggg cggcaacatc ggcctgccga tcctcggcgc
      541 caatccgctc gacgccggcg gggtctatgt gctggagctg tcgagctatc agatcgacct
      601 gacccattcg ctgcaatgcg atgtcgccgc gctgctcaac atcacgcccg atcaccttga
      661 ccggtacgat ggctttgccg gctatgccgc ggccaaggcg cggctgtttg cgatgcagcg
      721 ggccgatcag ttcgccattt tcggcatggg cgatgccaag acccaggcgg tcgccgcgcg
      781 cgagttgcag acccgcgccc ccggcaaggt ccgcagcgtt gacgggcggg acctcgccgc
      841 gctccagccc gactggccca gcctgcaagg gccccacaac ctgcagaacg cggccgtggc
      901 cgtggccatt gccgaggccc tgggcctgac cgcgatgcag tggcgccccg cgctggccag
      961 cttccgcggc ctgccgcacc ggatggagcg cgtcgccgaa gcgaacggcg tcatgtacat
     1021 caacgacagc aaggccacca acccggcctc gaccgcgccg gccctggcgg cgttcccgcc
     1081 caaccccaac cggcggatcc actggatcct gggcggcctg cccaagggcg atgacctgaa
     1141 cgaatgcgcg ccccatttcg gcaacatcgc cgccgcctat accatcggcg acgccggccc
     1201 gcgctttgcc gagctgctca gcccgcacat gccggtgacc cgcagtgaga tgatgtgcga
     1261 ggcgatccag caggccatgg ccgcggccca gcccggcgat gtcgtgctgc tctcgccggc
     1321 ttgcgccagc tttgaccagt tccgcgatta cgaggcgcgc ggcgatgcct ttcggcagat
     1381 cgtccagacc ctgctgaccc ccgaacccgc cggggccacg ggcggagggc gcggctgatg
     1441 gccagcaatc cccccctggt gccgcgcggc ggcaaggttg gcgtcgaagg cggcgcggtg
     1501 cgcgccaagc gcaaccgccg cggcgaactg gcgatctggt ggcgcgagat cgaccggacc
     1561 atgctggcgc tggtcctgac gctaatggcg atgggcgcgg cggcggttgc ggccagctcg
     1621 ccggccagcg cgcggcgcct gtcgaccgcg tcggtccggc tggatgacct gcacttcttc
     1681 tacctccaca tccgctatca gatcctgggc ctgatcgcga tgttcggggc cagcatgctg
     1741 ccgcgcgaca tggcccggcg ggccgggatc gtgctgtgct ttgccatgct ggtcgggctg
     1801 atgctggttc cggtaatcgg gttcagcgtc aacggcgcgc ggcgctggct taacctgggc
     1861 atgtcgctgc aaccctcgga attcctgaag ccggcctttg cgatcggcat ggcctggatc
     1921 ctctcatggc gggcccgcga tcccaatctg ccggtgatcg agatgtcgac cgggctgatg
     1981 atcctgatcg ccggcctgct gatgatgcag cccgatttcg gttcggcgat cctgttcatc
     2041 ggggtatggt tcgtcatggt gctgatgagc ggcatttcgc tgaagcggat cggcctcgcc
     2101 ggcggggcgg gggtgggctt catcgtgttg gcctacatgt tctatgagaa cgcccgcaac
     2161 cggatcgact ccttcctggg cggcggcacc gccttcgatc aggtcgacct ggcccagcgg
     2221 accctgctgg ccggcggctg gaccggatcg gggctatggc tgggcacccg caagctgggc
     2281 ctgcccgaag cccataccga ctacatcttc tcggtgatcg gcgaagagtt cggcctgatc
     2341 gcctgcgccc tgatcgtgat gatctatctc gcgctggtca cccgggtgct gatccggctg
     2401 ctggacgagg aagacctgtt caccaccctg gccgcggccg ggctgatcgc ccagatcggc
     2461 gggcaggctt tcatcaacgt gctggtcaac ctgcagctgt tcccgtccaa ggggatgacc
     2521 ctgccgctga tctcctacgg cgggtcgtcc accatcgcgc tgtgccttgg ggtgggcttc
     2581 ctgctgtcga tcacccggcg caaccccttc atcaagcgcg agccgttcaa cctcgccagc
     2641 cttggaatca agctatgagc ggcgctaccc gccactatgt cctggccgcc ggcggcaccg
     2701 gcgggcacct gatcccggcc ttcgccctgg cggtggagct ggaacggcgc ggccaccacg
     2761 tggcgctgat caccgacgat cgcggcagcc gcctgccggg caagccgcat tcgctggtcg
     2821 cccatgtcct gccggccggg cggatcggca agaacccgct gaagtggccc aaggggatcg
     2881 aggcgatcct cgaaggacgg cgcatggcgc tg
//
LOCUS       DASUDV010000367         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_940213,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000367 DASUDV010000000
VERSION     DASUDV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940213"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..547
                     /gene="dksA"
                     /locus_tag="VFV30_08055"
     CDS             89..547
                     /gene="dksA"
                     /locus_tag="VFV30_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX4848082.1"
                     /translation="MASVLGEEIDVLAKAKRALGGDYAPSESEPYMGEAQLDYYRRLL
                     LEWKKLILSAAQDTLHQLQEGPIREPDLNDRASSETDWGIELRTRDRQRKLIAKIDSA
                     LRRIEEGEYGYCEVTGEPIGLGRLSARPIATMTVEAQEAHERREKISRDD"
     gene            668..1027
                     /locus_tag="VFV30_08060"
     CDS             668..1027
                     /locus_tag="VFV30_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4848083.1"
                     /translation="MSDSEHRQLGRDSLFVLADLRIDGVQGEERIRVRNLSAGGLMAQ
                     GPCKVQRGLVGWINIRNIGWVEGSVAWVQDDRFGFAFREEIDPRLARAPISPGETTPR
                     FVRPPLSQFDPSEIRKI"
     gene            1109..2578
                     /locus_tag="VFV30_08065"
     CDS             1109..2578
                     /locus_tag="VFV30_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4848084.1"
                     /translation="MPLRLARHASLALVLVLGGCLGDDDRPIEVAAIGPSASPFAPGP
                     RLPGPAQLLRAATVEGLVGFDEQGRVVPALADRWIVTDDGLSFIFRLRDGTWADGSPI
                     TGESARAALIQAFAAQRGTALGKDLAVVAEVRTMAGRVIELRLSAQQPELLQLLAQPE
                     LGLVRGGRGAGPMRLRREKDSALLRPIPPEDRGLPQEEDWADRARKLELNAMPAKTAI
                     ERFNTGDADVVLGGALADFPRLDAMGVARGAIRMDPVSGLFGLALVHEDGFLAKPENR
                     EALAMAIDREALAGAINLAGWLVTTRVVNPGLPGDDGTIGERWAGRSLEERRALATSR
                     VTAWKGRSGGAVSLRIALGPGPGHDLLFERIAADFKAIGLSAVRVGADADADLRLIDT
                     VATYSSAQWFLNRLSCASLPAACSPAADRFAEQALTEPDPAKRAELFSQAEAQLTVAN
                     SYIPFGAPVRWSLVGGGVTGFAPNRLAVHPLMSLAMLPK"
     gene            2621..3037
                     /locus_tag="VFV30_08070"
     CDS             2621..3037
                     /locus_tag="VFV30_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX4848085.1"
                     /translation="MVEKGTARRMTAELPLGTDPQSVRARIEAVERLLEGMIEVPGLG
                     RKVGLDAIVGLVPVVGDVITAGMGLYIVWEARNLGMPKWQLWRMAGHVGFDSLIGAVP
                     VAGDLFDLVYRSNSRNLKIVRKHLDKHHPTTRVLEG"
     gene            3194..>3318
                     /locus_tag="VFV30_08075"
     CDS             3194..>3318
                     /locus_tag="VFV30_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX4848086.1"
                     /translation="MRVLLIEDEPTTARAIELMLTTEGFNVYSTDLGEEGLDLGKL"
BASE COUNT          539 a         1083 c         1155 g          541 t
ORIGIN      
        1 gcgcgctcga accggcgccg ggaggccatc gggaaagccc gaggccgcag gaccccgcac
       61 cacccggccg gtttcgtttg gagagtttat ggcgagcgtt ctaggcgaag aaatcgatgt
      121 tctggccaag gccaagcggg ccctgggggg tgactatgcc cccagcgaga gcgagcccta
      181 catgggcgaa gcgcaactgg actattaccg ccgcctgctg ctggaatgga agaagctgat
      241 cctctcggcc gcgcaggaca cgctgcacca gctgcaggaa gggccgatcc gcgagcccga
      301 tctcaacgat cgcgcctcga gcgagaccga ctgggggatc gaactgcgca cccgtgaccg
      361 gcagcgcaag ctgatcgcca agatcgactc ggccctgcgc cggatcgagg aaggcgagta
      421 cggctattgc gaagtgaccg gcgaaccgat cggcctgggc cggctttccg cccgtccgat
      481 cgcgaccatg acggtcgagg cgcaggaagc ccacgaacgc cgcgaaaaaa tttcccgcga
      541 cgattgagag acttggtccg cacaacaggg gtttgcgggc cgttaacccc ttttttgccg
      601 ttcccgttgt aactctgtcg accctgaatc cgttaattcg gatgccagcg aacttcagag
      661 gacggcaatg agcgacagcg aacatcggca actgggacgg gacagcctgt tcgtcctcgc
      721 cgatcttcgg atcgacgggg tccagggcga agagcgcatt cgcgtgcgca acctttcggc
      781 gggcggcctg atggcgcagg gcccgtgcaa ggtccagcgc ggcctggtcg gctggatcaa
      841 catccgcaac atcggctggg tcgaaggctc ggtcgcctgg gtgcaggatg accggttcgg
      901 ctttgccttc cgcgaggaaa tcgatccccg cctcgcgcgc gcgccgattt cgcccggaga
      961 aaccaccccg cgctttgtcc ggccgccgct cagccagttc gatcccagcg aaatccgcaa
     1021 gatctgaacg cgccgcccgc gctgttcgcg ggtcagtgcg tcaagcggct gtgcctgctg
     1081 ccgataatcg gctagaaccc ctttcactat gccgcttcgc cttgcccgcc atgcctctct
     1141 tgcgcttgtg ctggtgctgg gcggatgcct tggcgacgat gaccggccga tcgaagtggc
     1201 cgcgatcggg ccttcggcct cgcctttcgc gcccggcccg cgtctgccgg gtccggccca
     1261 gctgctgcgc gccgccacgg ttgaagggct ggtcgggttt gacgagcagg gccgcgtggt
     1321 cccggcgctg gctgaccgct ggatcgtgac cgacgacggg ctcagcttta ttttccgcct
     1381 gcgtgacggc acctgggccg atggcagccc gatcaccggc gaaagcgccc gcgcggcgct
     1441 gattcaggct ttcgcggccc agcgcgggac ggccctgggc aaggacctgg ccgtggtggc
     1501 cgaagtgcgg accatggccg gccgggtgat cgagctgagg ctgtccgccc agcagccgga
     1561 actgttgcaa ttgctggccc agccggaact ggggctggtg cgcggcggcc gcggggccgg
     1621 gccgatgcgg ctgcgccgcg aaaaggacag tgcgctgctg cgcccgatcc cgccggaaga
     1681 ccgcgggctg ccgcaggaag aggactgggc cgaccgggcc cggaagctgg aactcaacgc
     1741 gatgccggcc aagaccgcga tcgaacgctt caacaccggc gatgccgatg tggtgctggg
     1801 cggggcgctg gccgacttcc ccaggctcga cgccatgggc gtggcccgcg gcgcgatccg
     1861 gatggatccg gtctcgggcc tgttcggcct ggccctggtc cacgaggacg ggttcctggc
     1921 caagcccgag aaccgcgagg cgctggccat ggcgatcgac cgcgaggcgc tggccggggc
     1981 gatcaacctg gccggctggc tggtcaccac ccgcgtggtc aatcccggcc tgcccggcga
     2041 tgacggcacg atcggcgaac gctgggccgg gcgcagcctg gaagaacggc gcgcgctggc
     2101 cacctcgcgg gtcacggcct ggaaggggcg ctccggcggc gcggtctcgc tgcggatcgc
     2161 gctggggccg gggccgggtc acgacctgct gtttgagcgc atcgccgccg atttcaaggc
     2221 gatcgggctc agcgcggtcc gggtcggcgc ggatgccgat gccgatctgc ggctgatcga
     2281 cacggtcgcg acctattcca gcgcgcagtg gtttctcaac cggctgagct gcgccagcct
     2341 gcccgcggcc tgcagcccgg cggccgaccg gtttgccgaa caggccctga ccgagcccga
     2401 tccggccaag cgggccgagc tgttctcgca ggccgaagcc cagctgaccg tggcgaacag
     2461 ctacattccc ttcggcgcgc cggtccgctg gtcgctggtc ggcggcgggg tgaccggctt
     2521 tgcgcccaac cggctggcgg tccatccctt gatgtcgctg gccatgcttc ccaaatagaa
     2581 caggccggca ccgtgcccgg ccacgcgaag ggtcgttgcg atggttgaaa agggtactgc
     2641 ccggcggatg accgccgaac tgccgctcgg aaccgatccc caatcggtcc gcgcccggat
     2701 cgaggcggtc gagcgtttgc tggaaggcat gatcgaagtt cccgggctgg gccgcaaggt
     2761 cgggctggat gcgattgttg gcctggtgcc ggtggtgggc gatgtgatca ccgccgggat
     2821 ggggctctat atcgtctggg aagcgcgcaa cttgggcatg ccgaaatggc agctgtggcg
     2881 gatggcgggt catgtcgggt ttgacagcct gatcggcgcg gttccggtcg cgggcgacct
     2941 gttcgacctg gtctaccgct cgaacagccg caacctcaag atcgtgcgca agcacctcga
     3001 caagcaccat ccgaccaccc gggtgttgga aggctaggcc ggacgcgcac cgagccgtac
     3061 cacgcgactc gccggattcc ctgcgtttac gagaaattaa cctttctctt tcattggtcc
     3121 tatggttaat gacgagcaat gaccgttgcg aaacggttat cgcttaggga aacggggcag
     3181 aaggggacca cccatgcgcg tattgctgat cgaggacgag ccgaccacgg cccgcgccat
     3241 cgaactgatg ctgacgaccg aaggcttcaa cgtctattcg accgacctgg gcgaagaagg
     3301 cttggatctc ggcaagct
//
LOCUS       DASUDV010000368         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1293837,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000368 DASUDV010000000
VERSION     DASUDV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293837"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..540
                     /locus_tag="VFV30_08080"
     CDS             109..540
                     /locus_tag="VFV30_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4848087.1"
                     /translation="MIGMEILKPLAILAGWTMVMWLWMYATRIPAMSAAKIDTKNLTG
                     GTGKDLEAVLPGKVQWIAHNYNHLHEAPTVFYAVALALALIGQGDGLNATLAWAYVGL
                     RIAHSLVQAVWNRVMVRFMLFALSSLALMALTLHLLLAVFH"
     gene            complement(706..>2674)
                     /locus_tag="VFV30_08085"
     CDS             complement(706..>2674)
                     /locus_tag="VFV30_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848088.1"
                     /translation="YPADARVVNMILKDNFASRRVELEYGLPTRGGFGEWEGEATLLR
                     ITGPRRISLTATASDRSPLFEAERGVILPAGSAPGGLDPARFRSLIADSRDFGVNAAL
                     TTGLGKDGLDGSFSLNAAANRSDSKSWQGLDPVTLVDPLLQTQRTTTLQGGAGYANRL
                     GNWQLNSTLDATYSTADTYTDRSALVRDKASVQSLRIEGLATLSGSPFRMPAGDASAT
                     LKAGFTSIGFDSSDQRSSAGPVSLNRFRKLGGINLALPLTSRRDGGLGAIGDLSLNLG
                     AELSHLSDFGAVNGWNAGLTWGITGKLGLQGSYLVDEAAPTISQLGGPQIVTANVPVY
                     DFVSGQTVLVSVTNGGNPALRKETRRDWKFGATWQLPVLSGSSAVIEYIRNRSDDVSA
                     NFPLLTPQIEAAFPGRITRDGTGKLVAIDSRPVTFAQQTSSRIRWGFSLSDTLGKAAP
                     GGGGGGMAMRPGGPGPGQRPPGAGGGRPGGGGGGRMMAMMMGGGGQGRWSLGVYHTVQ
                     LDSRVLIAPGVPLLDLLDGDSLSANGTPRHAIEFNGGLFHKGKGMFFQGTWNAPTTLV
                     ASGVPGSSDLRFGAVTKANVNFFVELGDQGKLAEKSPLFKGLRLSLRIENLFDSRQKV
                     TDANGLVPLGYQADYLDPRGRVIELELRKMF"
BASE COUNT          451 a          885 c          852 g          486 t
ORIGIN      
        1 gtcaacaccg tgccggtgcg cttcacgctg ttcattctct cgaccgtctg cctgctgctg
       61 ctggcgatcc gcgcggtcga acttaccctc atggcaggag cctgaacgat gatcggaatg
      121 gaaatcttga aaccgctggc gattctggcc ggctggacca tggtgatgtg gctgtggatg
      181 tacgccaccc gcatcccggc gatgagcgcc gcaaagatcg ataccaagaa cctgaccggc
      241 gggaccggca aggacctgga agcggttctg cccggcaagg tccagtggat cgcccacaac
      301 tacaaccacc tccacgaagc gccgaccgtg ttctacgcgg tggcgctggc gctggcgctg
      361 atcggccagg gcgatggact gaacgccacg ctggcctggg cctatgttgg ccttaggatc
      421 gcgcactcgc tcgtccaggc agtgtggaac cgggtgatgg tgcgcttcat gctgtttgcg
      481 ctgtccagcc tggcgctgat ggcgctgacc ctgcacctgc tgctggcggt gttccactga
      541 gtgatggggg cggcccatca gtgacctaac catttccgtt cgtgctgagc ttgtcgaagc
      601 actgcttttg ttcttttgcc ggatagcagg aagaagaaca gtccttcgac aagctcagga
      661 cgaacggatt tgctttggga agtttccgac ccctagaaca tcgtcctaga acatcttcct
      721 gagttccagt tcgatcacgc gtccgcgcgg atcgaggtaa tcggcctggt agcccagcgg
      781 caccaggccg tttgcatcgg tcaccttctg gcgcgaatcg aacaggttct cgatccgcag
      841 cgacaggcgc agtcctttga acagcgggct cttttcggcc agcttgccct gatcgcccag
      901 ttccacgaag aagttgacat tggccttggt cacggcgcca aagcgcagat cgctgctgcc
      961 cggaaccccg ctggcaacca gggtggtcgg cgcgttccag gtgccctgaa agaacatccc
     1021 cttgcccttg tggaacagcc cgccattgaa ttcgatcgcg tggcgcgggg tgccattggc
     1081 ggatagcgaa tcgccatcaa gcagatctag cagaggcacc cccggtgcga tcagcaccct
     1141 gctgtcgagc tgcacggtgt gatagacgcc aaggctccag cggccctgac cgccaccgcc
     1201 catcatcatt gccatcatcc ggccaccacc accgccgccg ggacgcccgc cgcccgcacc
     1261 gggagggcgc tggccgggcc cgggaccgcc ggggcgcatc gccatgccgc cgccaccgcc
     1321 gccgggtgcc gccttgccca gcgtgtcgct caacgagaag ccccagcgga tgcgcgagct
     1381 ggtctgctgg gcgaatgtta ccgggcggct gtcgatcgcc accagtttgc cggtgccgtc
     1441 gcgggtgatc cgacctggaa acgccgcctc gatctgcggg gtcagcagcg gaaagttggc
     1501 gctgacatca tccgagcggt tgcgtatata ttcgatcact gcgcttgaac cagacagcac
     1561 cggcaattgc caggttgcgc cgaatttcca gtcgcgccgg gtttccttgc gcagcgccgg
     1621 attgccgcca ttggtcacgc tgaccagcac ggtctggcca ctgacgaagt catagaccgg
     1681 gacattggcg gtgacgatct gcgggccacc cagctggctg atcgtcggcg ccgcctcgtc
     1741 aaccagatag ctgccctgca ggcccagctt gccggtgatc ccccaggtca gcccggcgtt
     1801 ccagccgttg accgcgccga aatcggacag atggctcagc tccgcgccaa ggttcaggct
     1861 aaggtcgccg atcgcgccca gtccgccatc gcgccggctg gtcagcggca aggccaggtt
     1921 gatcccgccc agcttgcgaa agcggttgag cgaaaccggc ccggcgctgc tgcgctgatc
     1981 gctgctgtcg aacccgatgg aggtgaagcc ggccttgagc gttgccgagg catcacccgc
     2041 cggcatccgg aacggcgatc cgctgagcgt cgccagccct tcgatccgca gcgactggac
     2101 actggccttg tcgcgcacca gcgccgagcg gtcggtatag gtatcggcgg tgctgtaggt
     2161 tgcatcgagg gttgaattga gctgccagtt gcccagccgg ttggcatagc ccgccccgcc
     2221 ctgaagcgtg gtggtgcgct gggtctgcag caggggatcg accagcgtga ccggatcaag
     2281 cccctgccac gatttgctgt cgctgcggtt ggccgcggcg ttaaggctga aactgccgtc
     2341 cagcccatcc ttgcccagcc cggtggtgag cgccgcattg acgccgaaat cgcgcgaatc
     2401 cgcgatcagc gaacggaacc gcgccggatc gaggccgcct ggggcgctgc ccgcgggcag
     2461 gatcaccccg cgttcggcct cgaacagcgg gctgcgatcg cttgctgttg cggtcaggct
     2521 gatccggcgc gggccggtga tccgcagcag cgttgcctcg ccctcccact cgccgaaccc
     2581 gccgcgggtg ggcaggccat attcaagttc gacccggcgg ctggcgaaat tgtccttgag
     2641 aatcatgttc accacccgcg catcggccgg atag
//
LOCUS       DASUDV010000369         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1139113,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000369 DASUDV010000000
VERSION     DASUDV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139113"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1383)
                     /locus_tag="VFV30_08090"
     CDS             complement(91..1383)
                     /locus_tag="VFV30_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX4848089.1"
                     /translation="MIRTIAYDITRLFVGPIFLTPRGIDRIDLALARRVFADADTRNL
                     GILPTPWGMRAYPAAVVRTMLDRLEQLWSEQVEPEHDPQLRWLVDHLRSPAAVRQADA
                     PRPARLALGQKIARQLAMLATTGLPLGKPVRSAVPKGAVYVNIGQFGLVVRPFFNWLV
                     DRRDVTCAIMLHDVIPIEYPHLVSATHVGNHTRMVETTARHADCLIYNTHAARVSVEA
                     ALRDFGRSGLPSLVRALPLQPAFEQVEASFAPLDGIDYCVVVSTIEPRKNHAMLLEAW
                     QHLIARLGSAAPHLVIVGALGYDAVRTLGPLETDAALREKVHIVSGLSSPALAALVLG
                     ARAMLCPSLAEGFGLPLLEAQALGVPTIASDIAAHREVANGGAVLLDAADVMGWVDAI
                     AGLPGAQGRGPIERSADLGEAAYCADVLGFLGDCRGSV"
     gene            1730..2827
                     /locus_tag="VFV30_08095"
     CDS             1730..2827
                     /locus_tag="VFV30_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848090.1"
                     /translation="MPSRLKFISPTALATVILPTLLALLYFGLFASDVYISESRFVVR
                     SPEKSTASPLGMILNAGGLSSAGEENFAVVDYIRSREALKHVDRDKLVRNAYGSTDIS
                     IFDRFGGPFGRTSEEHLYRYFNEKVLIEFDTASHVTTLTVRAYGADTARKLNERLLQQ
                     AEQLVNRLSERARSDAIASAETEVAEAREKARNATVALARFRNQSGIIDPEKQAAINL
                     QMVSKLQDALLAEQTQLAQLEAYTPRNPQIPATRTRIKMIKAEIDRQSSFVAGAQGSL
                     SAAASQYQQLVFDAEFAGKQLAVALAALQEARNEARKKQVYVERIAEPNLPDYPLEPR
                     RLRGILATFVLGLLAWGVATTLLAGIREHRD"
     gene            2827..>3453
                     /locus_tag="VFV30_08100"
     CDS             2827..>3453
                     /locus_tag="VFV30_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export ABC transporter
                     permease"
                     /protein_id="HEX4848091.1"
                     /translation="MALAVDENPSFLRNLAVQRRVLAALFMREVLTRYGRHNLGFLWL
                     FVEPMIFTLGVTALWTATKSVHGSDLPIVAFALTGYSSVLLWRNMPGRCIGALWNNLS
                     LMYHRNIRVLDIYLARLMLEFGGATISFVTLAIVFITIGWLDPPQDVLQIVGGWLIIA
                     WFGSALAIWLGALSHESELVDKLWHPASYLIFPLSGSAFMVDALPREAQ"
BASE COUNT          597 a         1121 c         1126 g          609 t
ORIGIN      
        1 ggcgcggccc tgtcgaactc gttcgcattc ggcggggtca atgcctgcct ggtgctgacc
       61 gcggccgact gatcgcgcat gtccaggctg tcagacgctt ccccggcaat cgccaagaaa
      121 gccgagaaca tcggcgcaat aagcggcctc gcccagatcg gccgaccgtt cgatcgggcc
      181 gcgaccctgt gcgcccggca gtccggcaat cgcatcgacc cagcccatca catccgcggc
      241 atcgagcagg accgctccgc cgttcgccac ttcgcggtgc gcggcgatgt ccgatgcgat
      301 cgtgggaacg cccagcgcct gcgcctcgag cagcggcagc ccgaaaccct cggccagcga
      361 agggcacagc atggcccggg cgccaagcac cagcgcggcc agcgccgggc tggacaggcc
      421 cgatacgata tggacctttt cgcgcagggc cgcgtcggtc tcgagcggtc caagcgtgcg
      481 cacggcgtcg tatcccaggg cgcccacgat cacgaggtgt ggcgccgcgc tgcccaaccg
      541 cgcgatcagg tgctgccagg cttccagaag catcgcgtgg ttcttgcgcg gttcgatcgt
      601 ggagacgacc acgcaatagt ctatcccgtc gagcggcgcg aaactggcct cgacctgttc
      661 gaaggccggt tgcagcggca gcgctcggac caggctggga aggccggaac ggccgaaatc
      721 gcgcagcgcc gcctcgacgc tgacccttgc ggcatgggtg ttgtagatca ggcaatcggc
      781 atggcgcgcg gtggtttcga ccatgcgggt gtgattgccg acatgggttg cgctgaccag
      841 gtgcggatat tcgatcggga tcacgtcgtg gagcatgatc gcgcaggtca cgtcgcggcg
      901 gtctacaagc cagttgaaga aggggcgcac caccagcccg aactgcccga tgttgacata
      961 gaccgcaccc ttcgggaccg cgctgcgcac cggcttgccc agcggcaggc cggtcgtggc
     1021 cagcatcgca agctgccggg cgatcttctg gccgagcgcg agcctggccg gcctgggcgc
     1081 atcggcctgg cggaccgccg cgggcgagcg caggtgatcg accagccagc gcagctgcgg
     1141 gtcatgttcg ggttcgacct gctcggacca gagctgttcg agccgatcga gcatggtgcg
     1201 caccacggca gcgggatagg cgcgcatgcc ccacggcgtg ggaagaatgc ccagattgcg
     1261 cgtatcggca tcggcaaaga cccggcgggc cagggccagg tcgatccggt cgatcccgcg
     1321 cggcgtcagg aagatcggcc cgacgaacag ccgggtaatg tcataggcaa tggtgcggat
     1381 catgggcgat cggtgggagc cgggatgggg cgctctgtcg tttcggggat ggctgcgcgg
     1441 ccagaggtga aagctctcgc tagtcgcgtc aaaatagagg gtcaagacaa gtcgtgcctg
     1501 tgactgttcg cgcaaatact tcgcacgact ggcaaatgtt acggcttgcc ggcaattgcc
     1561 cggcgtggcg caagagtgcg ggaaaattgc cctttatctc gggacttgtc tcgggcaaga
     1621 caggcttgtg gggcgccgcg ctggggtgga ccgggcctgt cgatccgtca gcctgtattg
     1681 cccggaaacc cgtgccagtc cgcctgccat ccaacaaaga gttgcccctg tgcccagccg
     1741 cctgaagttc atcagcccca ctgccctggc cacggtcatt ctgccgaccc tgctggcctt
     1801 gctctatttc ggcctgttcg cttcggacgt ctatatttcc gaatcccggt tcgtggtccg
     1861 cagcccggag aagtcgacgg catcgccgct tggcatgatc ctcaacgcgg gcggactttc
     1921 cagcgccggc gaggaaaact tcgcggtggt tgactacatc cgttcgcgcg aggcgctcaa
     1981 gcacgtcgat cgcgacaagc tggtgcgcaa tgcctatggc tcgactgaca tttcgatctt
     2041 cgatcgcttc ggcgggccat tcggccggac cagcgaagag cacctctatc gctacttcaa
     2101 cgaaaaggtg ctgatcgaat tcgatacggc gagccacgtc accacgctga ccgtgcgcgc
     2161 ctatggcgcc gacaccgcgc gcaagctgaa cgagcggctg ctccagcagg ccgagcaact
     2221 ggtcaaccgg ctgagcgagc gggcccgcag cgacgcgatc gccagtgctg aaaccgaagt
     2281 ggccgaagcc cgggaaaagg cgcgcaatgc caccgtggcc ctggcccgct ttcgcaacca
     2341 gagcgggata atcgatccgg aaaagcaggc ggcaatcaac ctgcagatgg tctcgaagct
     2401 gcaggacgcg ctgctggcag agcagaccca gttggcccag cttgaagcct ataccccgcg
     2461 caatccccag atcccggcca cccgtacccg gatcaagatg atcaaggccg agatcgatcg
     2521 gcaatcctcg ttcgtcgcgg gcgcgcaggg ttcgctttcg gccgcagcgt cgcaatacca
     2581 gcagctggtg ttcgatgccg aatttgccgg caagcagctc gccgtagcgc ttgccgccct
     2641 gcaggaagcc cgtaacgaag cgcgcaagaa gcaggtctat gtcgaacgga tcgccgagcc
     2701 gaacctgcct gactatccgc tcgaaccgcg gcgacttcgc ggcattctgg ccaccttcgt
     2761 cctggggctg ctggcctggg gtgttgccac gacgctgctt gcaggcattc gtgagcaccg
     2821 tgactgatgg cgctcgcggt tgacgagaat ccttcctttc tgcgcaacct ggcggtgcag
     2881 cgcagggtgc ttgccgccct gttcatgcgc gaggtgctga cccgctacgg gcggcacaat
     2941 ctcgggttcc tgtggctgtt cgtcgagccg atgatcttta ctcttggggt tacagcgctg
     3001 tggaccgcga ccaaatcggt ccatggcagc gacctgccga tcgtggcctt tgccctgacc
     3061 ggttattcca gcgtgctatt gtggcgcaac atgccggggc gctgcatcgg cgcgctgtgg
     3121 aacaaccttt cgctgatgta ccaccgcaac atccgggtgc tcgacatcta cctggcgcgg
     3181 ctgatgctcg aattcggcgg tgcgaccatc tcgttcgtga cgctggcgat cgtcttcatc
     3241 acgatcggct ggctcgaccc gccgcaggac gtgctgcaga tcgtcggcgg ctggttgatc
     3301 attgcctggt tcggctccgc actggccatc tggcttggcg cgctcagcca cgaaagcgaa
     3361 ctggtcgaca agctctggca tccggcctcc tacctgatct tcccgctgtc cggatcggcc
     3421 ttcatggtcg atgccctgcc gcgcgaggcg cag
//
LOCUS       DASUDV010000370         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1139165,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000370 DASUDV010000000
VERSION     DASUDV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139165"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..578)
                     /locus_tag="VFV30_08105"
     CDS             complement(144..578)
                     /locus_tag="VFV30_08105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX4848092.1"
                     /translation="MAARKILVLNGPNLNLLGTREPEIYGHDTLADIEARLTARAAEL
                     GFALECRQTNHEGVLVDWLHEARGAVHAVLLNAGAYTHTSIALLDAIKATQLKVIEVH
                     LSDPETREPFRHLSYVGMAAIDCVKGLGAKSYEVALERAAAL"
     gene            complement(652..960)
                     /locus_tag="VFV30_08110"
     CDS             complement(652..960)
                     /locus_tag="VFV30_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848093.1"
                     /translation="MRGPKPILTKPGASLALTAVAAVLLAGCSGRDSESAEKVAAINA
                     ASARAEAAAKRAEEAAAKIEKATAPTVIEVDPDATEDAEDAAIAEQNEPVAPDADIKG
                     "
     gene            complement(1059..1472)
                     /locus_tag="VFV30_08115"
     CDS             complement(1059..1472)
                     /locus_tag="VFV30_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HEX4848094.1"
                     /translation="MALLEALIGPLAAIIDKVIPDKGARDKAKLELLQLQGSQDLAEI
                     NARLSAIVAEANSVDPWTSRARPSFLYVIYILLLWALPMGLLAAFSPGKAREIADGMN
                     AYLSGLPEPLYALFGTGYLGYTAARQWGKIAGSDK"
     gene            1624..>2541
                     /locus_tag="VFV30_08120"
     CDS             1624..>2541
                     /locus_tag="VFV30_08120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX4848095.1"
                     /translation="MPPVTVVMPVYNALPYLDEAVESILGQSYSAFEFAIYDDHSTDG
                     SYERALEWARRDPRIRVARGERRLGPSASSNAAAAMARSEFVARMDADDIAVPDRLAR
                     QLAVLQADPGAALTGSIFDLIDSDGNCFSRAPGRRSRGAGPPIAHASLFYRRAAFEAA
                     GGYRPNTDYFEDIDLYRRMARHGAVVVIDRPLLKLRFAGQHARLRDDREQVVESINLH
                     YRSAGSARPGPRNLSPVAFYSVAVLAALGLQRPALLGLMARRAHFDRPHLTLAILGFV
                     ALAELSPAFARGLNQLLLRLRARTARAQTG"
BASE COUNT          384 a          827 c          880 g          450 t
ORIGIN      
        1 accagtttgc tgtcgatgtt catgccgccc gaccggctgg aacccttggt ttcgctcatg
       61 agattgtctt ctctggcctc tattcagaaa cagggcttcc gcctatgcgg cgggctggcg
      121 gcagaggcaa gcctaaagcg cccctaaagt gcggccgcgc gctccagcgc cacttcatag
      181 ctcttcgcgc cgaggccctt gacgcagtcg atcgcggcca tgccgacata ggacaggtgg
      241 cggaacggct cgcgcgtttc gggatcggag aggtggacct cgatcacttt cagctgggtc
      301 gccttgatgg catcgagcag cgcgatcgag gtatgggtat aggccccggc gttgagcagc
      361 acggcgtgga ccgccccgcg tgcttcgtgc agccaatcga ccagcacgcc ttcgtggttg
      421 gtctgccggc attccagcgc aaagcccagc tcggccgcac gcgcggtaag tcgggcttcg
      481 atatcggcca gcgtgtcatg gccatagatc tccggctcgc gggtgccgag caggttgaga
      541 ttggggccgt tgagaaccag gatcttgcgc gcagccacgg gctttccttg cggagttcgg
      601 gattgcgcca agcatggccc gcgccgcggc ggcgggcaag ccgaaagggg atcagccctt
      661 gatgtcggcg tcgggggcga ccggctcgtt ctgttcggcg atggccgcgt cttcggcgtc
      721 ttcggtcgca tccggatcga cttcgatcac ggtcggcgcg gtggccttct cgatcttggc
      781 ggcggcttcc tcggcgcgct tggcggcggc ttcggcccgg gccgaggcgg cgttgatcgc
      841 ggcaaccttt tcagccgatt cggaatcgcg ccccgaacag ccggccagca gcacggcagc
      901 cactgccgtc agggccagcg aagcgcccgg cttggtcagg atcggtttcg gtccgcgcac
      961 agcatactcc ttgatccggc cgttgcagga ccggtctggc aaagggccat aacgcagaaa
     1021 gattacccga aactaaccaa acgcgggcgc ggggagggtt atttgtcgct gccggcgatc
     1081 ttgccccact ggcgcgcagc cgtgtagcca aggtagccgg tgccgaacag cgcgtagagc
     1141 ggctcgggca ggccagagag gtacgcgttc atcccgtcgg caatctcgcg cgccttgccc
     1201 gggctgaagg cggccagcag gcccatcggc agggcccaga gcagcaggat gtagatcaca
     1261 tagaggaagc tgggccgggc gcggctggtc cagggatcga ccgaattggc ctcggccacg
     1321 atcgcggaga ggcgggcgtt gatctcggcc aggtcctgcg atccctgcaa ttgcagcagt
     1381 tccagcttgg ccttgtcgcg cgcgcccttg tcgggaatga ccttgtcgat gatcgccgcc
     1441 agcgggccga tcagggcttc gagtagtgcc atggtgagtt cctgtggtta ttagggggat
     1501 gccgaaataa ccaatacggt tcctgtagga aagttcgcag cgcgcggcac ggatggaacg
     1561 cgggcagggc gggggttaac agccgccgct gcacggtgta tcggcggggc gatggaaccc
     1621 ggtgtgcccc ccgttacggt cgtcatgccg gtctacaacg ccttgcccta tctcgatgaa
     1681 gcggtcgaaa gcatcctcgg gcagagctac tccgcctttg aattcgcgat ctacgacgat
     1741 cactccactg acggttccta tgaacgcgcg ctggaatggg cgcggcgcga tccgcggatc
     1801 cgggttgcgc ggggcgaacg gcgcctgggc cccagtgcca gctccaacgc ggcggcggcg
     1861 atggcgcgca gcgagttcgt ggcgcggatg gatgccgacg atatcgcggt gcccgaccgg
     1921 ctggcccggc aacttgccgt gctgcaggcc gatcccggcg ccgccctgac cgggtcgatc
     1981 tttgacctga tcgacagcga cggcaactgc ttcagccgcg cccccggcag gcgttcccgc
     2041 ggagccgggc cgccgatcgc ccatgccagc ctgttctacc gccgcgcggc cttcgaggcg
     2101 gctggcggct atcggccgaa caccgactat ttcgaggata tcgatctcta ccgccgaatg
     2161 gcccggcacg gggccgtggt ggtgatcgac cggccgctgc tgaagctgcg ctttgccggc
     2221 cagcacgccc ggctgcggga tgaccgcgaa caggtggtcg agtcgatcaa cctgcactac
     2281 cgctcggcgg gcagcgcccg gccgggcccc cgcaatctct cgccggtggc gttctattcg
     2341 gtggcggtgc ttgccgcgct cggcctgcag cgcccggcct tgctgggcct gatggcacgc
     2401 cgcgcgcact ttgaccggcc tcacttgacc ctggcgatcc tgggcttcgt ggcgctggcc
     2461 gaactctctc cggcctttgc gcgggggctg aaccagttgc tgctgcggct ccgcgcccgg
     2521 accgcgcgcg cgcagaccgg g
//
LOCUS       DASUDV010000371         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_100824,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000371 DASUDV010000000
VERSION     DASUDV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100824"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFV30_08125"
     CDS             <1..889
                     /locus_tag="VFV30_08125"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX4848096.1"
                     /translation="GAGRFECWRKDRDQHRGGQEPGRRVPPALPCPCRSRSAGHVAAA
                     ILKRGCKFIQLPTTLLAQVDSSVGGKTAINTAAGKNLVGAFHQPSLVLADLAVLDTLP
                     RRELLAGYAEVVKYGLLGDADFFAWCEANGAAMLAGDAPLREYAVAHSVAAKARIVQA
                     DEKETLGLRALLNLGHTFGHALEAQTGFSDRLLHGEGVALGMVLAARYSARRGLIPAA
                     SAERIARHFAEVGMASEIAALGLNCDGRALVAHMLHDKKMDAGTLPFVLLRGIGEAFL
                     AKDVALDDVAAFLDGELAG"
     gene            946..1362
                     /locus_tag="VFV30_08130"
     CDS             946..1362
                     /locus_tag="VFV30_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848097.1"
                     /translation="MILVELALILFLAFRKAGGSCLLRLLLSAMAFMALLMALLSLAV
                     LLGEGRTIDFADNDGPFFIGLAIALGIIAIWLASMRDESPSKPPQGQTENAGDAADPP
                     PKRSLFFDMIGCALLGAIALVALTVLIAAGIGLAGR"
     gene            complement(1372..>2612)
                     /locus_tag="VFV30_08135"
     CDS             complement(1372..>2612)
                     /locus_tag="VFV30_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848098.1"
                     /translation="IVTDLARQLVQDYRVPLAYAERSDEPERHRVAAYRFVRCPNGKG
                     SERGLGDAYNLSPTPADRNLCTLTTYEPLPANPVVAQTVRERITVDGVSTLRFRILVR
                     RPDGQTASVVYFAPRNEVQHNKLGAEVLASALGLPKRRPGDPAPPGKDLVDTWIEASR
                     QRMTAATGQMVDVLAATGKLPTGSNFVAQRVDPQAISRNAGPLMQQLDRGLGDDQSGV
                     ETAVITRTLALLPEADWRREAPGILAIVSRHRPLDADLHGDLMLRLVDLGPAAIPLFA
                     RASAAQGLPDTVLVGACKFGAAAQPALGPRVIEAWRRANLPQLKRRPSRSAFTGYRQC
                     VKDGERNGPPEDIAFSPCWRQATLSDRDVRIYLTLKRMGLGAQADAMARHDRAQKFAS
                     KYAGVGPNSPATICLPDPAR"
BASE COUNT          412 a          878 c          859 g          463 t
ORIGIN      
        1 tggcgcaggt cgattcgagt gttggcggaa agaccgcgat caacaccgcg gcgggcaaga
       61 acctggtcgg cgcgttccac cagccctccc ttgtccttgc cgatctcgat ctgctgggca
      121 cgttgccgca gcgatcctca agcgcgggtg caagttcatc cagctcccca ccacgctgct
      181 ggcccaggtc gattccagcg tcggcgggaa gaccgcgatc aacactgccg ccggcaagaa
      241 cctggtcggc gcgttccacc agccctcgct ggtcctggcc gatctggccg tgctcgatac
      301 cctgccccgg cgcgagctgc tggccggcta tgccgaagtg gtgaagtacg gtctgctggg
      361 cgatgccgac ttcttcgcct ggtgcgaagc gaacggcgcg gcgatgctgg ccggcgatgc
      421 ccctcttcgc gaatatgccg tggcccattc ggtggcggcc aaggcccgga tcgtccaggc
      481 ggacgagaag gaaacgctgg gcctgcgcgc gctgctcaac ctcggccaca cctttggcca
      541 cgcgctggag gcgcagaccg gcttttcgga ccgcctgctc cacggcgaag gcgtcgcgct
      601 cggcatggtc ctcgccgccc gctactcggc ccgccgcggc ctgatcccgg cggccagcgc
      661 cgagcggatc gcccggcact tcgccgaggt cggcatggcc agcgagatcg ccgccctcgg
      721 cctcaattgc gacggccgcg cgctggtggc gcacatgctc cacgacaaga agatggacgc
      781 gggcaccctg cccttcgtcc tgctgcgcgg gattggcgag gcgttcctgg cgaaggacgt
      841 cgcgctggac gacgtggcgg cgtttctgga tggggagctg gcgggttaaa aacccgatca
      901 attcggcgga atgcgatgga cacttttctg agctggcttt tcagcgtgat cctggtcgag
      961 ctcgcgctga tcctgttcct tgccttccgc aaggcaggcg gttcctgtct ccttcggctc
     1021 ctgctttcgg cgatggcctt catggccctg ctgatggctt tgctcagcct cgcagtactg
     1081 cttggcgagg gtcggacgat cgactttgcc gacaacgacg gcccgttctt catcggtctc
     1141 gcgatcgccc ttggcatcat cgcgatctgg cttgcaagca tgcgggacga atcgccttcc
     1201 aagccaccgc aaggtcagac ggaaaacgcc ggcgacgcgg ccgatccccc gcccaaacgg
     1261 tcgctgttct ttgacatgat cggctgcgct ttgctcggcg ccatcgcctt ggtcgcgctg
     1321 accgtgctga tcgcggccgg gatcgggctg gctggccgat agggtcggca gctaccgggc
     1381 gggatccggc aaacaaatgg tcgcaggcga attgggcccc acaccggcat acttgcttgc
     1441 gaacttttgc gcccggtcgt gccttgccat cgcatcggct tgcgccccca ggcccatccg
     1501 cttcagcgtg aggtaaatcc ggacgtcgcg atcggagagg gtcgcctgcc tccagcacgg
     1561 gctgaacgcg atgtcctcgg gcggaccgtt gcgttcgccg tctttcacgc attgccgata
     1621 gccagtgaag gcagagcgcg acggacgcct tttcaactgg ggcaggttcg cccggcgcca
     1681 cgcctcgatc acccgcgggc ccagggctgg ctgggccgca gcgccgaact tgcaggcgcc
     1741 gaccagaacc gtatccggca agccctgagc ggcactggcc cgcgcaaaca gcgggatcgc
     1801 ggcggggccc agatcgacca accgcagcat caggtccccg tgcaggtcgg catcgagcgg
     1861 ccgatggcgg ctcacgatag ccagaattcc gggcgcttcg cggcgccaat cggcctcggg
     1921 cagcaaggcc agcgtgcgcg tgatcacggc cgtttccacg ccagattgat cgtcacccag
     1981 accgcgatcg agctgctgca tcagcggacc cgcatttcgt gaaatcgcct gcggatcaac
     2041 ccgctgggcg acaaagttgc tgcctgtcgg caacttgccg gtcgccgcca gcacatcgac
     2101 catttggcca gtcgcggcgg tcatgcgctg gcggcttgcc tcgatccatg tgtcgaccag
     2161 atccttgccc ggcggggcgg gatcgcctgg ccttcgtttg ggcaagccca gcgcggaggc
     2221 aaggacctct gcgccaagct tgttgtgctg aacctcgttg cgcggcgcaa aatagacgac
     2281 cgaggcggtc tgcccatcgg gacgccggac aagaatgcgg aaccgaagag tgctcactcc
     2341 atcgaccgtg atccgctcac ggaccgtctg ggcaacgacc ggattcgccg gaaggggctc
     2401 gtaggtggtc agcgtgcaga gatttcggtc agccggggtc ggggacaggt tgtaagcatc
     2461 gccaaggcct ctctccgaac ctttgccatt cggacagcgc acgaaccgat aggctgccac
     2521 gcggtgccgc tccggttcat cgctgcgttc ggcgtaggcc agcggcacgc ggtagtcctg
     2581 caccagttgc cgggccagat cggtgacgat gg
//
LOCUS       DASUDV010000372         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1028668,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000372 DASUDV010000000
VERSION     DASUDV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028668"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1243
                     /locus_tag="VFV30_08140"
     CDS             68..1243
                     /locus_tag="VFV30_08140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX4848099.1"
                     /translation="MTPQARVQAAIEVLDLVIAAARQGGASADRIVAEWFRTRRFAGS
                     GDRRAVRDLVYRAIRAAGEVPQGGRAAMLLLARDDPALAALFDGSRHAPEPIADGEPV
                     ARAGIAPAWLEAALKASGIEADEAAALLGRAPLDVRINRLKPVSGALPLPGEPTQAPD
                     ALRFAFGAPVEQWDAYKSGAIEVQDTGSQLACAALQAQPGETVIDLCAGAGGKTLALA
                     AAMDNRGELIACDTDRARLSRLAPRAERAGASTIAERLLNPGREAENLTDLAGKADAV
                     LIDAPCSGAGTWRRNPEARWRLTPKELERLTAIQARLLDVATGLVRPGGRIVYATCSL
                     LDGEGADQIEAFVRRHPDWRASLPVLGAGRPRGAGLRLTPAHDRTDGFFIARLDWLC"
     gene            1279..1788
                     /locus_tag="VFV30_08145"
     CDS             1279..1788
                     /locus_tag="VFV30_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4848100.1"
                     /translation="MRYTPAALALALLVAVSSSTGFSAPATPLDPRAADLVAQGRAAL
                     LAGDTNGAIDAYEAALAIQPGHVAIVLNLAEAARKQGMQGKALHYYRAALKLDPNNQY
                     AIAGEGEALVEKGALEKAKTNLARLQQLCGNTSCGPAQQLSAAIERGPTPQVVSAEAV
                     EAKPVVSEN"
     gene            complement(1785..2285)
                     /locus_tag="VFV30_08150"
     CDS             complement(1785..2285)
                     /locus_tag="VFV30_08150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX4848101.1"
                     /translation="MSDPFAHLPYRPCVGVMLVNPSGQVFVGKRIDTRGQPAEGGDFW
                     QMPQGGVDDGEDLREAALGELWEETGVEERHITLLAQTAEELLYDLPDELMGKLWKGR
                     YRGQRQHWFLARFSGEDADIHLERHDPPEFTEWRWIEPDLLPDLIVPFKKRVYRAVLE
                     EFRELI"
BASE COUNT          361 a          774 c          804 g          355 t
ORIGIN      
        1 accgttgccc aagaagcggg acccccgcct tcgcgggggt gacggtcggt aggacggggg
       61 agggcctgtg actccccagg cccgcgttca agccgcgatc gaggtgctcg atctcgtcat
      121 tgccgcggcg cggcagggcg gggccagcgc cgacaggatc gtcgctgaat ggttccgcac
      181 ccgccgcttt gccggatcgg gtgatcggcg cgcggtccgc gatctggtct accgcgcgat
      241 ccgcgccgcg ggcgaagtgc cgcaaggcgg acgggccgcc atgctgctgc tggcccggga
      301 cgacccggcg cttgccgcgc tgttcgacgg atcgcgccat gcgccagagc cgatcgcgga
      361 tggcgagccg gtggccaggg cagggatcgc cccggcctgg ctcgaagccg cgctcaaggc
      421 cagcgggatc gaggccgatg aagccgccgc gctgctcggc cgcgccccac ttgatgtgcg
      481 gatcaaccgg ctgaagcctg tgtccggcgc gctgccactg cccggcgagc cgacccaggc
      541 ccccgatgcg ctgcgctttg ctttcggtgc gccggttgag caatgggatg cctacaagtc
      601 cggcgcgatc gaggtgcagg acaccggatc gcagcttgct tgcgcggcgc tgcaggcaca
      661 gccgggcgag accgtgatcg acctgtgcgc cggagcgggg ggcaagacct tggccctggc
      721 ggcggcgatg gacaaccgcg gcgagctgat cgcctgcgat accgatcggg cccggctttc
      781 ccggctggcg ccgcgcgccg agcgcgccgg ggcaagcacc atcgccgaac gcctgctcaa
      841 cccaggccgc gaggcggaga acctgaccga tctggccggc aaggccgacg cggtgctgat
      901 cgacgcgccc tgttcggggg ctggcacctg gcgccgcaat cccgaggcgc gctggcggct
      961 gacgcccaag gaactggagc ggttgaccgc gatccaggcg cgtctgctcg acgttgcgac
     1021 cgggctggtc cggcccggcg ggcggatcgt ctacgccacc tgctctctgc tcgatggcga
     1081 aggcgccgat cagatcgagg cgttcgttcg ccgtcatccc gattggcgcg ccagcctgcc
     1141 ggtgctgggt gccgggcggc cgcgcggagc cgggctgcgg ctcactccgg cacacgatcg
     1201 caccgacggc tttttcatcg cacgtttgga ttggctgtgt tagctctgcc gcaaatgccg
     1261 accttcaagg agcagatgat gcgttacacc cccgccgccc ttgcgttggc gctgctggtc
     1321 gcggtgagtt cgagcacggg cttctccgcg cccgccaccc cgcttgatcc gcgcgccgcc
     1381 gatctggtcg cccagggccg tgccgccttg ctggccggcg ataccaacgg cgcgatcgat
     1441 gcctatgaag ccgcgctggc aatccagccg ggccatgtcg cgatcgtgct gaacctggcc
     1501 gaagccgcac gcaagcaagg gatgcagggc aaagcgctgc attactatcg cgcggcactg
     1561 aaacttgatc ccaacaacca gtacgcgatc gcgggcgaag gagaggctct ggtcgaaaag
     1621 ggcgcgctgg aaaaggccaa gaccaatctg gcgcggctgc agcagctgtg cggcaacacc
     1681 agctgcggcc cggcccagca gctttccgct gcgatcgaac gcgggcccac cccgcaggtg
     1741 gtgagcgcgg aggcggtaga ggccaagccg gtggtgagcg agaattagat cagttcccga
     1801 aactcttcca gaacagcgcg atagacgcgt ttcttgaagg gaacgatcag atcgggcagc
     1861 aggtcgggtt cgatccagcg ccattcggta aactcgggcg gatcgtggcg ttcgaggtgg
     1921 atgtcggcat cttcgccgga aaagcgcgcc aggaaccagt gctggcgctg gccgcggtat
     1981 cggcccttcc atagtttgcc catcaactcg tcaggcaggt cgtagagcag ttcctcggca
     2041 gtctgggcca gcagtgtgat gtgccgctcc tcgaccccgg tttcttccca cagttcgccc
     2101 agcgccgctt cgcgcaggtc ttccccgtca tcgaccccgc cctggggcat ctgccagaaa
     2161 tcgccgccct cggccggctg gccgcgggtg tcgatccttt tgccaacgaa gacctggccg
     2221 gacggattga caagcatcac ccccacgcag ggacgatagg gaaggtgggc aaagggatcg
     2281 gacatgcggg cccc
//
LOCUS       DASUDV010000373         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1337610,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000373 DASUDV010000000
VERSION     DASUDV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337610"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFV30_08155"
     CDS             <1..610
                     /locus_tag="VFV30_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX4848102.1"
                     /translation="CIFPVHEFPDKLHTVGFPAPGHFLKVLDEQGNEVPPGTPGELVG
                     KSPTMMLGYKNQPEKTREGYWIDPVDGSTWQRMGDVGRIDADGFVELVGRTKDVIISG
                     GFNIFPIDLETELAKDERVVEAAVVGMPSDAWGETPVGFVVLKPGVGEDSLEDIRATA
                     NSRLGKTQRLSALHAIAEMPRSHIGKLLKTELREMISASPAQ"
     gene            complement(590..1813)
                     /locus_tag="VFV30_08160"
     CDS             complement(590..1813)
                     /locus_tag="VFV30_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848103.1"
                     /translation="MPSTQRASITVVFAIVLLDMLGFGVIMPVLPQLIMHLGQMEVDS
                     AAVFAGWLSAGYAVMQFVFAPIIGGLSDRFGRRPVLLASVLAMAVDYTIMGLAPALWW
                     LVIGRIVAGMTGASWSAAYAYIADVTPPEKRAANFGLMGMAFGLGFILGPALGGLLGA
                     IDPHLPFFASAALAFANFLFGFFILKESLPAQRRRPFDWARANAFAALKALRHQNRAV
                     LWFVAALGTWSLAHVVYPAVWSYVAIAAWQFDARQIGLSLAVVGLTSALVQGLGLRLL
                     LPRLGERRAVTMGVLAFAGSAALYAVAGSVAMIYLAIVVGGLQGFIQPSIQALNSRAV
                     DASSQGELQGATQAIGSIAMIVGPPLYSGILAKFSGERALVHLPTMPMLVAAGIAFVA
                     LALFWRGTVLLRRAG"
     gene            1922..>2506
                     /gene="guaA"
                     /locus_tag="VFV30_08165"
     CDS             1922..>2506
                     /gene="guaA"
                     /locus_tag="VFV30_08165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX4848104.1"
                     /translation="MSTQHSDSILIVDFGSQVTQLIARRVREAGVYCEIAPFSSAEAA
                     FARMQPKGIILSGSPAGVPEEGSPRAPQMLFEAGLPILGICYGQQVMSHQLGGMVEAG
                     NKGEWEGEFGRAFITVTEDCALFEGLWAKGERHQVWMSHGDKVTRFAPGFRIVAVSDG
                     APFAVIADEERRFYGTQFHPEVAHTPDGAKLIANF"
BASE COUNT          487 a          851 c          802 g          366 t
ORIGIN      
        1 ctgcatcttc ccggtccacg aatttcccga caagctgcac accgtgggct ttcccgcgcc
       61 ggggcatttc ctcaaggtgc tggacgaaca gggcaacgag gtgccgccgg gcacgcccgg
      121 cgaactggtc ggcaaatcgc ccaccatgat gctgggctac aagaaccagc ccgaaaagac
      181 ccgcgagggc tactggatcg atcccgtgga cggatcgacc tggcagcgga tgggcgatgt
      241 cgggcggatc gatgccgatg gcttcgtcga actggtcggg cggaccaagg acgtgatcat
      301 ctcgggcggg ttcaacatct tcccgatcga ccttgaaacc gaactggcca aagacgaacg
      361 cgtggtcgaa gctgcggtgg tgggcatgcc cagcgatgcc tggggcgaaa ccccggtggg
      421 cttcgtggtg ctcaaaccgg gtgttgggga agacagcctc gaagacatcc gcgccaccgc
      481 caacagccgc ctgggcaaga cccagcggct ttcggcgctt catgccattg ccgaaatgcc
      541 gcgcagccac atcggcaagc tcttgaagac agaactgagg gaaatgatct cagccagccc
      601 ggcgcaatag aacggtcccg cgccagaaca gcgccagcgc gacaaaggca atccccgccg
      661 cgaccagcat cggcatggtc ggcaggtgga ccagcgcgcg ctcgccgctg aacttggcga
      721 ggatgcccga gtagagcggc gggcccacga tcatcgcgat agagccgatc gcctgggtgg
      781 cgccttgcag ttcaccctgg ctcgaggcat cgaccgcgcg gctgttgagc gcctggatcg
      841 aaggctggat gaagccctgc aggccgccca ctacgatcgc caggtagatc atcgctaccg
      901 atccggccac ggcatagagc gctgccgagc cggcaaaggc cagcaccccc attgtcaccg
      961 cgcgccgttc gcccagccgc ggcagcagca gcctgagccc cagtccctgg accagcgcgc
     1021 tggtcagccc gaccactgcc agcgaaagtc cgatctggcg cgcgtcgaac tgccaggcgg
     1081 cgatcgcgac ataggaccag accgccgggt agaccacgtg cgccaggctc caggtcccca
     1141 gtgccgccac gaaccacagc accgcgcggt tctggtgccg cagtgccttc agcgcggcaa
     1201 aggcattggc acgcgcccag tcgaacggtc ggcggcgctg cgcgggcagg ctttccttca
     1261 ggatgaagaa accgaacagg aagttggcaa aggctagtgc cgccgaagca aagaagggca
     1321 ggtgcggatc gatcgcgccc aacagcccac ccagcgccgg gcccaggatg aagccaaggc
     1381 caaaagccat gcccatcagc ccgaaattgg cggcgcgctt ttccggcgga gtgacatcgg
     1441 cgatataggc ataggctgcc gaccaactgg ccccggtcat ccccgccacg atccggccga
     1501 tcaccagcca ccacagggcc ggggccagac ccatgatcgt gtaatcgacc gccatcgcca
     1561 gcacactggc cagcagcacc ggccgccgcc cgaaccggtc ggacaggccc ccgatgatcg
     1621 gggcgaagac gaattgcatc accgcatagc cggcgctcag ccagccggcg aagaccgccg
     1681 cgctgtccac ctccatctgg ccaagatgca tgatcagctg cggcaacacc ggcatgatca
     1741 cgccgaaacc cagcatgtcg agcagaacga tggcgaagac cacggtgatc gaggcgcgtt
     1801 gggtggaagg caagtttcga tccccggctt gtgcccacgc gctctagccg cttgcggatt
     1861 gctgcgcaaa tcgcaccctg gcggccttgc cttggcggcc cctgttcctt atcggcccac
     1921 catgagcacc cagcattccg attccatcct catcgtcgat ttcggcagcc aggtaaccca
     1981 gctgatcgcc cgccgcgtgc gcgaggccgg ggtctattgc gagatcgccc ccttctccag
     2041 cgccgaagcc gccttcgcac ggatgcagcc caaggggatc atcctgtccg gttcgcccgc
     2101 cggggttccc gaggaaggat ccccgcgcgc gccgcagatg ctgttcgagg cgggcctccc
     2161 gatcctgggg atctgctatg gccagcaggt gatgagccac cagctgggcg gcatggtcga
     2221 ggccggcaac aagggcgaat gggagggcga gtttggccgc gccttcatca ccgtgaccga
     2281 ggactgcgcg ctgttcgagg ggctgtgggc caagggcgaa cgccaccagg tgtggatgag
     2341 ccacggcgac aaggttaccc gctttgcgcc cggtttcagg atcgtcgcgg tttccgatgg
     2401 cgcgcccttt gcggtgatcg ccgatgagga gcggcgcttt tacggcaccc agttccaccc
     2461 cgaggtggcg cacacccccg acggggcaaa actgatcgcc aacttc
//
LOCUS       DASUDV010000374         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1978669,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000374 DASUDV010000000
VERSION     DASUDV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978669"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFV30_08170"
     CDS             complement(<1..317)
                     /locus_tag="VFV30_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848105.1"
                     /translation="MALLESGLQRRAARAIERQLDYQRKRAAKLRAREDRLILEMQRH
                     SAAIRARLEAVRPIAPDAPVLEVGSGTHGIIFYFGTSNGVGIDPLADDYRALFPELHS
                     RART"
     gene            complement(350..1699)
                     /gene="accC"
                     /locus_tag="VFV30_08175"
     CDS             complement(350..1699)
                     /gene="accC"
                     /locus_tag="VFV30_08175"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX4848106.1"
                     /translation="MTISRILIANRGEIALRIHRAAHEMGIETVAVHSTADADAMHVR
                     LADHAVCIGPPAAKDSYLNVAAIISAAEITHADAIHPGYGFLSENAQFAEIVESHGIT
                     WIGPKPEHIRTMGDKIEAKRTAGALGLPLVPGSDGAVSDIAEAREIAEKAGYPVIIKA
                     ASGGGGRGMKVCNGPDELETLIQQAGSEAKAAFGDATVYIEKYLGNPRHIEFQVFGDG
                     NGNAIHLGERDCSLQRRHQKVLEEAPSPVISPEERDRMGGIVAKAMADMGYRGAGTIE
                     FLWENGEFYFIEMNTRLQVEHPVTEAITGMDLVREQIRIADGKPLSVAQEDIEFSGHA
                     IECRINAEDPWTFAPSPGLVSAYHPAGGMNVRVDSGLYAGYRIPPYYDSMIAKLIVKG
                     RTREGCIMRLKRALEEMVVSGVKTSIPLHQALMNEPDFIHGDYTIKWLEEWLAERAQ"
     gene            complement(1713..2207)
                     /gene="accB"
                     /locus_tag="VFV30_08180"
     CDS             complement(1713..2207)
                     /gene="accB"
                     /locus_tag="VFV30_08180"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX4848107.1"
                     /translation="MSETKGSSRSGGMNIDSKLVRELAELLAETGLTEIEVEDGDRKI
                     KVARQAAVQHFAAAPAAAPAAAPALPLASAAPEPAAAAAPAADANALKSPMVGTAYLT
                     PEPGAAPFVSVGSQVKAGDTLLIIEAMKVMNPITADKAGTIKAILVENAQPVEFDQPL
                     VVIA"
BASE COUNT          330 a          748 c          787 g          424 t
ORIGIN      
        1 gtcctggcgc ggctgtgcag ctctggaaac agggcgcggt agtcatcggc cagcggatcg
       61 atcccgacgc cgttgctggt cccgaaatag aagatgatgc cgtgggtgcc ggacccgact
      121 tcaagcaccg gcgcatcggg ggcgatcggc cggacggctt ccagccgcgc ccggatcgcg
      181 gccgaatggc gctgcatttc caggatcagc cggtcttccc gggccctcag cttggcggcg
      241 cgtttgcgct gatagtccag ctgccgctca atcgcccgcg ccgcgcgccg ctgcagtccc
      301 gattccagca gcgccatcgg cgcgccctag cgggacggcg cggagcggct cactgcgcgc
      361 gctccgccag ccactcttcc agccacttga tcgtgtagtc gccgtggatg aaatcgggct
      421 cgttcatcag cgcctggtgc agcggaatgc tggtcttgac gccgctgacc accatttcct
      481 caagcgcgcg cttcagccgc atgatgcagc cttcgcgggt ccggcccttg acgatcagct
      541 tggcgatcat ggaatcgtaa tagggcggga tccggtatcc ggcgtagagc ccggaatcga
      601 cccgcacgtt catgccgccg gccgggtgat aggcgctgac cagacccggc gagggggcga
      661 aggtccaggg atcctcggcg ttgatccggc attcgatcgc gtggccggaa aactcgatgt
      721 cctcctgcgc gaccgacagc ggcttgccat cggcaatgcg gatctgctcg cgcaccaggt
      781 ccatgccggt gatcgcttcg gtaacggggt gttccacctg caggcgggtg ttcatttcga
      841 tgaaatagaa ctcgccgttt tcccacagga attcgatcgt gccggcgccg cgatagccca
      901 tgtcggccat cgccttggcc acgatgccgc ccatccggtc ccgctcttcc gggctgatga
      961 cgggcgaggg ggcctcttcc agcaccttct ggtggcggcg ctggagcgag caatcgcgct
     1021 cacccagatg gatcgcgttg ccgttgccgt cgccaaacac ctggaattcg atgtggcggg
     1081 gattgcccag gtatttctcg atatagaccg tggcgtcgcc gaacgcggcc ttggcctcgc
     1141 tgccggcctg ctggatcagc gtttccagct cgtccggacc gttgcagacc ttcatgccgc
     1201 gcccgccgcc gccggaggcc gccttgatga tcaccggata gccggccttt tccgcgatct
     1261 cgcgggcttc ggcgatgtcg ctgaccgcgc cgtccgatcc ggggaccagc ggcaggccca
     1321 gcgcgccggc ggtgcgcttc gcctcgatct tgtcacccat ggtgcggatg tgttcgggct
     1381 tgggcccgat ccaggtgatc ccgtggctct cgacgatttc ggcgaactgg gcgttctccg
     1441 aaaggaagcc atagccgggg tggatcgcat cggcgtgggt gatttccgcg gccgagatga
     1501 tcgcggcgac gttgaggtag gaatccttgg ctgccggcgg cccgatgcag accgcgtggt
     1561 cggccaggcg gacgtgcatc gcgtcggcat cggcggtgga gtgcaccgcc accgtctcga
     1621 tccccatttc gtgggccgcg cggtggatgc gcagcgcgat ctcgccacga ttggcgatca
     1681 ggatgcggga aatggtcatg ggtgaagcgg ccttacgcga tcacgaccag cggctggtcg
     1741 aattcgaccg gctgcgcgtt ctcgaccagg atcgccttga tcgtgccggc cttgtcggcg
     1801 gtgatcgggt tcatcacctt catcgcctcg atgatcagca gggtatcccc ggccttgacc
     1861 tggctgccga ccgagacgaa cggcgcggcg cccggctcgg gcgtgagata ggcggtgccg
     1921 accatcggcg acttgagcgc gttggcatcg gccgcgggtg cggcggccgc ggcgggctcc
     1981 ggcgcggcgg aggccagcgg cagggccggg gcggcagcgg gcgcggccgc cggagcggcg
     2041 gcaaagtgct ggacggcggc ctgacgggcc accttgatct tgcgatcgcc gtcctcgacc
     2101 tcgatctcgg tcaggccggt ttcggcgagc agttccgcca gttcgcggac cagtttgctg
     2161 tcgatgttca tgccgcccga ccggctggaa cccttggttt cgctcatgag attgtcttct
     2221 ctggcctcta ttcagaaaca gggcttccgc ctatgcggcg ggctggcggc agaggcaagc
     2281 ctaaagcgc
//
LOCUS       DASUDV010000375         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_189552,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000375 DASUDV010000000
VERSION     DASUDV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189552"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFV30_08185"
     CDS             complement(<1..1217)
                     /locus_tag="VFV30_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4848108.1"
                     /translation="MVDISDLPSTRNLVELLLNRADARGDAPFLWAKQGGAWQPLSWN
                     EAVRQVCCLAESLRRLGLNDGDRVMLVSENRPEWCIADFAIMAAGCITVPAYVTNTER
                     DHQHILEDSGASAVIVANDKLAKPLIGAVLRSGQARHVIGIEPLRMSQTSNFEYHDWS
                     VLTAGDGAAARQAVNARIAGITRQDTACLIYTSGTGGAPRGVMQHHGAILMNADGAAR
                     VLAEDFGLDEDEAFLSFLPLSHAYEHTGGQMLPISVGAQIYYAEGLEKLASNIEETRP
                     TIMVVVPRLFEVLRTRIMKQIEKQGKVANYLMDRAMGIGSAKAAGRGRWRDKPMDFIL
                     EKTLRPKIRARFGGRMKAMVSGGAPLSPEVGVFFESMGLTMLQGYGQTEAGPVISCNR
                     PAAGLKMDTVGPPL"
     gene            complement(1305..>2339)
                     /locus_tag="VFV30_08190"
     CDS             complement(1305..>2339)
                     /locus_tag="VFV30_08190"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4848109.1"
                     /translation="GKNAREIVAKVSTAPRNPAPMVESDVNNYQAKQRPVVCATYRRH
                     RICSMGPPSSGATTLLATLIQLERFDLKALGKDNPVSWHLIAESQRLAYADRARYAAD
                     ADFVPVPVAGMVDRKYLAARSALIDPGKTLPKAVAGTPPGAEKLSFADSPQLEEYGTS
                     HFSVVDRAGNAVSYTSTIEGPFGSGIVAGGYFLNNELTDFDMNPELDGRLVANRVEAG
                     KRPRSSMTPTLVFGPDGRLRLVIGAAGGVTIPAQVIRGVIGVIDWDMPVQDALALPVI
                     FAPGTETVFIEQGSWLEGMIPALKALGHAEVQPRNLPLKANAIELRGSQPRGGADPRS
                     EGKAVSEAGA"
BASE COUNT          340 a          825 c          771 g          403 t
ORIGIN      
        1 agcggcgggc cgacggtgtc catcttcagg cccgcggcgg ggcggttgca gctgatcacc
       61 gggccggcct cggtctggcc atagccttgc agcatggtca gccccatcga ttcaaagaac
      121 acgcccactt ccgggctgag cggcgccccg cccgaaacca tcgccttcat ccgcccgcca
      181 aagcgcgcgc ggatcttggg ccgcagggtc ttttccagga tgaagtccat cggcttgtcg
      241 cgccagcggc cgcgcccggc ggccttggcg ctgccaatgc ccatcgcccg gtccatcagg
      301 taattggcga ccttcccctg cttttcgatc tgcttcatga tccgggtgcg cagcacctcg
      361 aacaggcgcg gcaccacgac catgatcgtc gggcgggttt cctcgatgtt gctggccagc
      421 ttttccagcc cttcggcgta atagatctgc gcgccgaccg agattggcag catctgcccg
      481 ccggtgtgct cataggcgtg gctgagcggc aggaacgaga ggaaggcctc gtcctcgtcc
      541 agcccgaaat cctcggccag gacgcgcgca gcgccgtcgg cgttcatcag gatcgcgccg
      601 tggtgctgca tcaccccgcg cggggcgccg ccggtgccgc tggtgtagat caggcaggcg
      661 gtgtcctggc gggtgatccc ggcgatccgc gcattgaccg cctggcgggc cgccgcgccg
      721 tcaccggcgg tcagcaccga ccagtcgtgg tattcgaaat tgctggtctg gctcatccgc
      781 agcggctcga tcccgatcac gtggcgggcc tggcccgacc gcaggacggc gccgatcagc
      841 ggcttggcca gcttgtcgtt ggcgacgatc accgcgctgg cgcccgaatc ctccaggatg
      901 tgctggtggt cgcgttcggt gttggtgaca taggccggca ccgtgatgca gcccgcggcc
      961 atgatcgcga aatcggcgat gcaccattcg ggccggtttt ccgagaccag catcacccgg
     1021 tcgccatcgt tcagccccag ccggcgcagc gattcggcca ggcagcagac ctgccggacc
     1081 gcctcgttcc agctcagcgg ctgccaggcc ccgccctgct tggcccacag aaagggcgcg
     1141 tcgccccgcg catcggcccg attgagaaga agctccacca gattccgggt ggagggaaga
     1201 tcactgatat caaccacgcg taccgcccct ttcgcagcac atcctgttcc cgcctatccg
     1261 aataggcggg cttttccgct gcggcaagcc gcctgcccct ctggtcaggc tccggcttcc
     1321 gaaacggcct tgccctcgct gcgcggatcg gcgccgccgc gcggctggct gccgcgcagt
     1381 tcgatcgcgt tggccttcag cggcaggttg cgcggctgga cttcggcgtg gcccagcgcc
     1441 ttcagcgccg ggatcatgcc ttccagccac gagccctgtt cgatgaagac ggtttcggtg
     1501 cccggggcga agatcaccgg caaggccagc gcgtcctgga ccggcatgtc ccagtcgatc
     1561 acgccgatca ccccgcggat cacctgggcc gggatcgtca cgcccccggc cgcgccgatc
     1621 accagccgca gccgtccgtc ggggccgaag accagggtcg gggtcatcga gctgcgcggg
     1681 cgcttccccg cctcgacccg gttggcgacc agccggccgt ccagttccgg gttcatgtcg
     1741 aaatcggtca gctcgttgtt gaggaaatag ccgcccgcga cgatgcccga tccgaacggg
     1801 ccctcgatcg tcgaggtata ggacaccgcg ttgccggccc ggtcgaccac cgagaagtgc
     1861 gacgtgccgt attcctccag ctgcggcgaa tccgcgaagc tcagcttttc ggcccccggc
     1921 ggggttccgg ccacggcctt gggcagggtc ttgcccggat cgatcagggc cgaacgcgcc
     1981 gccaggtact tgcggtccac catgcccgcc accggcaccg gcacgaaatc ggcatcggcg
     2041 gcatagcgcg cgcggtcggc ataggcgagc cgctggcttt ctgcgatcag gtgccacgac
     2101 accgggttgt ccttgcccag cgccttgagg tcaaaccgtt ccagctggat cagcgtggcg
     2161 agcagtgtgg tcgcgcccga gctgggcggg cccatcgagc agatccggtg gcggcggtaa
     2221 gtcgcgcaga ccaccggtcg ctgcttggcc tggtagttgt tgacgtcgct ctcgaccatc
     2281 ggtgcgggat tgcgcggcgc ggtgctgacc ttggcgacaa tctcacgcgc gttcttgcc
//
LOCUS       DASUDV010000376         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1603049,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000376 DASUDV010000000
VERSION     DASUDV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603049"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="leuC"
                     /locus_tag="VFV30_08195"
     CDS             <1..1120
                     /gene="leuC"
                     /locus_tag="VFV30_08195"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX4848110.1"
                     /translation="IRLIGDADAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLG
                     ALAFGIGTSEVEHVLATQTLLLKQSKTMEVRVEGQLGAGVTAKDVVLHICGVLGASGG
                     TGYVIEYTGSVIRDLSIEGRLTVSNMAIEHGARAGLIAPDDKTFAYVEGRPYAPKGAD
                     WDKAVAWWRSLATDPGAAYDKVVVIDAAAIAPTVTWGTSPEDTVAITGTVPDPASFAD
                     PSKQDAARKSLEYMGLVPGQRMQDVEVQNVFIGSCTNSRIEDLRAAAAVLKGRTKAPN
                     VKWAIVVPGSGLVKRQAEAEGLDRIFTEAGLEWREPGCSACLGMNPDKVPAGERCAST
                     SNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLADVREL"
     gene            1158..1610
                     /locus_tag="VFV30_08200"
     CDS             1158..1610
                     /locus_tag="VFV30_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848111.1"
                     /translation="MAFSDQPPPSLLRIWPLVPLGRWAARVVHRAEPLPDGGLLSAKH
                     FIPADTAWRLGQAYLGLLGLLAIPLSGLYLDEFHQTTGYFYHYTDILGRRVAEQRLGF
                     WSDLPSDLKVILPLTVVLHVVGLVSAARCLLVRRELLPASEAGRPGGQ"
     gene            complement(1604..>2293)
                     /locus_tag="VFV30_08205"
     CDS             complement(1604..>2293)
                     /locus_tag="VFV30_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4848112.1"
                     /translation="GRGVQTTVADAGLTAQRVQAGPPERLSDGRLRIGGLTAHSAVYA
                     CGPWLPKLFPDLLKDRIVATLQEVYHFGPAAGDGRFSPPALPVWADFNAGDIVYGFPG
                     LEGQGFKIAFDRHGAEVDPDSQDRRVSDEGVEQARAYLAKRFPGLAGAPFVHGRVCQY
                     ENSSNGDFLIDRLPGHDRVWLVGGGSGHGFKHGPAVGQRVAAHIADAALPVEPRFRLA
                     AKGRMQKRTVY"
BASE COUNT          345 a          812 c          790 g          346 t
ORIGIN      
        1 cattcgcctg atcggcgatg ccgatgccga acagggcatc gtccacgtgg tcggcccgga
       61 acagggcttt tcgctgcccg gcgcgacgat cgtctgcggc gacagccaca ccgcctgcca
      121 cggcgggctg ggcgcgctgg ctttcgggat cggcacgagc gaggtcgagc acgtgctggc
      181 cacgcagacc ctcctgctca agcagtcgaa gacgatggaa gtgcgcgtcg aaggccagct
      241 gggcgccggc gtgaccgcca aggacgtcgt cctgcacatc tgcggcgtgc tgggcgcgtc
      301 gggcggcacc ggctatgtca tcgaatacac cggatcggtg atccgcgatc tttccatcga
      361 agggcggctg accgtgtcca acatggcgat cgagcacggc gcccgcgccg gcctgatcgc
      421 cccggacgac aagactttcg cctatgtcga gggccggccc tatgccccca agggcgcgga
      481 ttgggacaag gcggtcgcct ggtggcgcag cctggcgacc gatcccggcg cggcctatga
      541 caaggtggtg gtgatcgatg ccgccgcgat cgcgcccacg gtcacctggg gcaccagccc
      601 ggaagacacc gtggcgatca ccggcaccgt ccccgatccc gccagctttg ccgacccgtc
      661 caagcaggac gccgcccgca agagcctgga atacatgggc cttgtgcccg gccagcggat
      721 gcaggacgtg gaagtgcaga acgtcttcat cggatcgtgc accaacagcc ggatcgagga
      781 cctgcgcgcc gccgccgcgg tgctgaaggg ccggaccaag gcgcccaacg tcaagtgggc
      841 gatcgtcgtg ccgggctccg gcctggtcaa gcgccaggcc gaggccgagg ggctggaccg
      901 gatcttcacc gaagcgggcc tggaatggcg cgagccgggc tgttcggcct gcctgggcat
      961 gaacccggac aaggtccctg ccggcgaacg ctgcgcctcc acgtccaacc gcaacttcgt
     1021 tggccgccag ggcccgggtg cccgcaccca cctggtcagc cccgcaatgg ctgccgcagc
     1081 ggcggtgacc gggcggctgg cggacgtgcg cgagctctag gttgcgggcg caccggccca
     1141 tcagctagcc tgccggcatg gctttcagcg atcagccgcc cccttcgctt ttgcgcatct
     1201 ggccgctggt cccgctgggc cgctgggctg cccgcgtggt gcaccgcgcg gagcctttgc
     1261 ccgacggcgg attgctgagc gcgaagcatt tcatcccggc agacaccgcc tggcggctgg
     1321 gccaggccta tctcggcctg ctgggcctgc tcgccatccc cctgtccggt ctctacctcg
     1381 acgaattcca ccagacgacg gggtatttct atcattacac tgacatcctg ggccgccgcg
     1441 ttgccgaaca gcggctgggg ttctggagcg atctgccgtc ggacctcaag gtcatcctgc
     1501 cgctgaccgt ggtgctccac gtcgtcggcc tggtctcggc cgcgcggtgc ctgctggttc
     1561 gcagagagct gctgccggcc agcgaggccg gacggcccgg cggtcagtaa accgtccgtt
     1621 tctgcatcct gcccttcgcg gccaggcgga agcgcggttc gaccggcagc gccgcatcgg
     1681 caatgtgcgc cgccacgcgc tgacccacgg ccgggccgtg cttgaacccg tggcccgatc
     1741 cgcctccgac cagccagacc cggtcatgcc ccggcagtcg gtcgatcagg aaatcgccgt
     1801 tggagctgtt ctcgtactgg cagacccggc cgtggacgaa gggcgcgccg gccaggcccg
     1861 ggaaccgctt ggccagatag gcccgggcct gttcgacccc ctcgtccgaa acgcgccggt
     1921 cctggctgtc gggatcgact tcggcgccgt ggcggtcaaa ggcgatcttg aagccctgcc
     1981 cttccagccc cggaaagcca tagacgatgt ccccggcgtt gaaatcggcc cagaccggca
     2041 gggccggcgg tgagaagcgc ccgtcgcccg cggccgggcc aaagtgatag acctcttgca
     2101 gggtggcgac gatccggtcc ttgagcagat cgggaaacag cttgggcagc cacggcccgc
     2161 aggcgtagac cgcggagtgg gcggtaaggc cgccgatccg caagcggcca tcggaaagcc
     2221 gctccggcgg tccggcctgc acgcgctgcg cggtgaggcc tgcatcggcc accgtggtct
     2281 gcacgccgcg ccc
//
LOCUS       DASUDV010000377         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1713471,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000377 DASUDV010000000
VERSION     DASUDV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713471"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="VFV30_08210"
     CDS             complement(<1..1693)
                     /locus_tag="VFV30_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX4848113.1"
                     /translation="MSSDPAGAAAAPQPDKPVSEPAPARNLGPLRMIWRETAKYPRQV
                     IFALMALTVTATATLAIPAGFKMVVDKGFAKGGDIDSIAQSFRYLLMIVGVLGLGTAV
                     RFYFVSWLGERVVADIRTKVQGNLLRLSPGFFEENSPNEISSRMTADTALIEQVVGTT
                     VSVALRNVIMGIGGVIYLFSLAPLLTGVLLAGIVGVLFPIMAFARRIRTISRTSQDRV
                     ADIGTIVAETLGGMKVVQAFNQEQRELERFAGAVELTFATAKRRILLRAVITALAVSA
                     IFGAIVLLVWEGAKGVAAGDITGGTIVAIVITSGVVAGAFGALTEVYGDLLRGAGAAS
                     RLAELLGEKPSIAAPERPLQLPSPPRGKLSFEKVTFRYPTRLEVAALADFSLSIEPGE
                     TVAIVGPSGAGKSTLFQLAERFYDPQAGTIKLDGVPLTAVDPAEVRRRMALVPQEGVL
                     FAASARDNLRYGNWDASEEAIWDAARAANAEGFLRDLPEGLDTFLGEGGARLSGGQRQ
                     RIAIARAVLRDAPILLLDEATSALDAESEQLVQQALDRLMANRTTLVIAHRLATVR"
     gene            1839..>3068
                     /gene="phaZ"
                     /locus_tag="VFV30_08215"
     CDS             1839..>3068
                     /gene="phaZ"
                     /locus_tag="VFV30_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX4848114.1"
                     /translation="MLYTAYELQRSWLNAASTWASVGAQMLNNPDLPFSYGGAGSVMA
                     SALEVFAHASANYGKPAFDIESVTVDGAEHPVTEAIVLHKPFGNLLRFTHTGLPKDAP
                     RLLIVAPMSGHFATLLRGTVARMLERHEVFVTDWADAKMVPLSAGEFDLDDYIDYLIE
                     YFEHIGPGAHALAVCQPSVPTLAATAIMADTKHPCRPLTLTMMGGPIDTREAPTSVND
                     LAMQRPIEWFRHTVIATVPLQYPGAGRRVYPGFLQLAGFMSMNLGNHMMSHYHMFKHL
                     VAGADEDADATKAFYDEYRAVCDLNAAYYLQTVEEVFQKHSLPNGTFVHRGKTVDLGK
                     IKDTAILAVEGERDDISGIGQTKAALHLATGLAEAKKQYHLAPEVGHYGIFNGSKWRD
                     RIAPVLEDWIARFDRPAL"
BASE COUNT          538 a         1063 c          998 g          469 t
ORIGIN      
        1 tgcgcacggt cgccaggcgg tgggcgatga ccagggtagt gcggttcgcc atcagccggt
       61 caagcgcctg ctggaccagc tgctcgcttt cggcatccag cgccgaggtc gcttcgtcca
      121 gcagcaggat cggcgcgtcg cgcagcaccg cgcgggcaat cgcgatccgc tggcgctgcc
      181 cgcccgaaag ccgggccccg ccctcgccca ggaaggtgtc gagcccttcg ggcagatcgc
      241 gcaggaagcc ctcggcgttg gcggcgcggg cagcgtccca gatcgcctct tcgctggcat
      301 cccagttgcc atagcgcagg ttgtcgcggg ccgaggcggc aaacagcacg ccttcctgcg
      361 ggaccagcgc catgcggcga cgcacttcgg ccggatcgac cgcggtcagc ggcacgccgt
      421 ccagcttgat cgtcccggcc tggggatcgt agaaccgctc ggccagctga aacagggtgg
      481 acttgcccgc gcccgagggg ccgacaatcg ccaccgtctc gcccggttcg atggacagcg
      541 aaaagtcggc cagcgcggcc acttccagcc gggtcggata gcggaaggtg accttctcga
      601 agctcagttt gccgcgcggc gggctgggca gctgcaaggg ccgctccggc gccgcaatgc
      661 tgggcttctc gcccagcagc tcggccaggc ggctggccgc cccggccccg cgcagcagat
      721 cgccgtagac ttcggtcagc gcgccaaagg ctccggccac caccccggag gtgatcacga
      781 tcgccacgat cgtccccccg gtaatgtccc cggcggcaac gcccttggcc ccttcccaga
      841 ccagcaggac aatcgccccg aagatcgcgc tgactgccag cgcggtaatc accgcgcgca
      901 gcaggatccg gcgcttggcc gtggcaaagg tcagctccac cgcgccggcg aaccgctcca
      961 gctcgcgctg ttcctggttg aaggcctgaa ccaccttcat gccgccgagc gtttcggcca
     1021 cgatcgtccc gatatcggcg acgcggtcct ggctggtgcg ggaaatggtc cggatccggc
     1081 gcgcgaaggc catgatcggg aacagcacgc cgacgatccc ggcgagcaga acgccggtca
     1141 gcagcggagc cagcgaaaac aggtagatga ccccgccgat ccccatgatc acgttgcgca
     1201 gcgccaccga aacggtggtg ccgacaacct gttcgatcag cgcggtatcg gcggtcatcc
     1261 gcgaggatat ctcgttcggg ctgttctcct cgaagaaccc gggcgaaagc cgcagcaggt
     1321 tgccctgcac cttggtgcga atatcggcca ccacccgttc gcccagccac gagacgaaat
     1381 agaaccgtac cgccgtgccc agccccagga cgccgacgat catcagcaga tagcggaacg
     1441 actgggcgat cgaatcgata tcgccgcctt tggcaaagcc cttgtcgacc accatcttga
     1501 acccggccgg gatcgccagg gtggccgtgg ccgtgacggt cagcgccatc agggcaaaga
     1561 tcacctggcg cggatacttg gccgtttcgc gccagatcat ccgcagcggg cccagattgc
     1621 gcgccggagc gggttcggaa accggtttgt caggctgcgg cgcggcagcg gcgccggcgg
     1681 gatcggaact catcggcgcg ccctagcaca tggacggcgc ggcgtaaggg ccaaatcgcg
     1741 ccgccttgaa ttgcctgcct attgtgcgct gcggcatttt gtgcgttgca caatcgggca
     1801 attccggcca gtgtcccggc aacaggagga cgcgggacgt gctttacacc gcttacgaat
     1861 tgcagcgctc gtggctcaac gccgccagca cctgggcctc ggtcggcgcg cagatgctca
     1921 acaacccgga cctgccgttc agctatggcg gggcgggatc ggtcatggcc tcggcgctgg
     1981 aggtctttgc ccacgcttcg gccaattacg gcaagccggc cttcgacatc gaaagcgtga
     2041 ccgtggacgg cgccgagcat ccggtgaccg aagcgatcgt gctacacaag ccgttcggca
     2101 acctgctgcg cttcacccac accggcctgc ccaaggacgc accgcggctg ttgatcgtgg
     2161 cgccgatgtc ggggcacttc gccaccctgc tgcgcggcac ggtcgcgcgg atgctggaac
     2221 ggcacgaggt cttcgtgacc gactgggccg atgccaagat ggtgccgctg tcggccgggg
     2281 aattcgatct cgatgactac atcgattacc tgatcgaata cttcgagcat atcggcccgg
     2341 gcgcccatgc tctcgccgtc tgccagccgt cggtgccgac gcttgccgcc accgcgatca
     2401 tggccgatac caagcacccc tgccggccgc tgaccctgac catgatgggc ggcccgatcg
     2461 acacccgcga agcgccgact tcggtcaatg acctggccat gcagcgcccg atcgaatggt
     2521 tccgccacac cgtgatcgcc acggtgccgc tgcagtatcc gggcgcgggc cgccgggtct
     2581 atccgggctt tctgcagctg gccgggttca tgagcatgaa cctgggcaac cacatgatga
     2641 gccactacca catgttcaag catctggtgg ccggcgccga cgaggacgcg gacgcgacca
     2701 aggcgttcta tgacgaatac cgcgcggtct gtgatctcaa cgccgcctac tacctccaga
     2761 cggtggaaga ggtgttccag aagcacagcc tgcccaacgg caccttcgtt caccgcggca
     2821 agaccgtgga cctgggcaag atcaaggaca ccgcgatcct ggcggtcgag ggcgagcgcg
     2881 acgacatttc cgggatcggc cagaccaagg ccgcgctgca cctggcgacg ggcctggcgg
     2941 aagcgaagaa gcagtaccac ctggccccgg aagtcggcca ctacggcatc ttcaacggca
     3001 gcaagtggcg cgaccggatc gccccggtgc tggaagactg gatcgcccgg ttcgaccggc
     3061 cggcgctg
//
LOCUS       DASUDV010000378         6272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1559172,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000378 DASUDV010000000
VERSION     DASUDV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559172"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFV30_08220"
     CDS             complement(<1..543)
                     /locus_tag="VFV30_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX4848115.1"
                     /translation="MSWPEFLGWLDTNALDVGGYHISLLGGLKVVTVAVLVLVAGWLL
                     NRLVRRFFRRMNRLDLGQQVLGEKLVGVVVWTALVLIGIDALGINLTALTVFSGAFGL
                     AIGFGLQKTLGNLISGIILLMDRSIKPGDVIAVGSGSDKTVGKVDRIGMRAVAVTTRD
                     RIKYMIPNELLMTSAVENWTA"
     gene            complement(540..1751)
                     /gene="metC"
                     /locus_tag="VFV30_08225"
     CDS             complement(540..1751)
                     /gene="metC"
                     /locus_tag="VFV30_08225"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX4848116.1"
                     /translation="MAKDPPDNLGPATRLVTAGRRPEWTGPVVNPPVWRASTHLYEDS
                     AAQQAGLHVNKDGVFHYGARGGPTQWALADALTALEPGAAGTMLYPTGLAAPVMALLA
                     VLKTGDEVLVSDNSYDPSRTFAEKGLRDYGITARYFDPYDIDGFAAAITDKTRAVLLE
                     NPGSVSMEMCDVPALAAAAKARGLVVLLDNTWAGPTGFQALKHGVDLSIVALTKHVGG
                     HSDLMLGAVTANAEWYPRLRRHSLMLGQMAAPDDCALALRGLRTMGVRLERSCASALQ
                     IAEWLQDRPEVAAVLFPMLPGAPGHELWKRDFTGGCGLMTIIMAGKDLKARDAFIDAL
                     SLFGIGYSWGGFESLALGSHPERDRDVMPWPPRGIAPENRHAVRLWIGLEEPADLIAD
                     LERGLAAWAAA"
     gene            complement(1758..2597)
                     /gene="sseA"
                     /locus_tag="VFV30_08230"
     CDS             complement(1758..2597)
                     /gene="sseA"
                     /locus_tag="VFV30_08230"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX4848117.1"
                     /translation="MESLVSTEWLANEMGASDLRIVDCTRHLPGTGRDAQAEYEAGHI
                     PGAVFMDLGDLVDTNAPVENTLPPAEKFASRMQSLGLGDGSRIVLYDDSPVKTSARAW
                     FMLKMFGAHDVAILDGGLAKWKAEGRPLTQGKESLRHRHFTVWQDDKNVRTKAHVLAN
                     LHSKDEQVVDARGAKRWSGEEADPRPNIASGHIPGSFNVPFTDVYNADGTFKDPAAIR
                     ATFEAAGVDMGKPVITSCGSGVTACVLIFALHLAGKDDAALYDGSWTEWGNDPDTPKA
                     VKA"
     gene            complement(2709..3965)
                     /locus_tag="VFV30_08235"
     CDS             complement(2709..3965)
                     /locus_tag="VFV30_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848118.1"
                     /translation="MNKTGGRLTAASAVALMLALAPAPGLAQSGGWQLPGATVQPAGP
                     KAQGPVDRDAPVVTKAVPRPTQSATPAAPSPSPSAAQTPTASAPSPTATPARGAAQRP
                     AASAPRQAGAPAAATPAPATAAPAGTALPPATAPATGPAAPGAFPTGFAPVPPPASTA
                     APAAAAAAAGWYWPWIAGLAALLALVFAGLWWSRRREERPLQITFERPIVPQPVPAAA
                     PQPLPPVGAAAAPAPVGAAAVGAAPARVLPEPHLGLGLPAGLGITLEARRMNASLVAT
                     TLNYVLRLTNTGSEALTALAVEGDMVSAHASLPPEAQIADDKQRLEPRHALVELAPGE
                     SAEFTGDFRLPLTAVTPIRAGEAAYFVPLARLRVAARTAAGESLVQVQTYVVGELPEA
                     SGGALKPFRLDLGPRTYSRLGQRAVN"
     gene            4087..4545
                     /gene="queF"
                     /locus_tag="VFV30_08240"
     CDS             4087..4545
                     /gene="queF"
                     /locus_tag="VFV30_08240"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX4848119.1"
                     /translation="MADTPSPLHLGQQSALPASPEAAVLDYVPNPRPGALFLVRFAAP
                     EFTSLCPVTGQPDFAHLVIDYAPGETIVESKSLKLFLGSFRNHAGFHEDVTVGIGQRL
                     FAEMKPQWLRIGGYWYPRGGIPIDVFWQSGAAPDGLWVPDQGVAGYRGRG"
     gene            4660..>6272
                     /locus_tag="VFV30_08245"
     CDS             4660..>6272
                     /locus_tag="VFV30_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848120.1"
                     /translation="MRFTSTLRTATASFALAASLATAAPALAQDNAEDGEGLSEIIVT
                     AQRREENQKDVPISVATVRGDALAAVSGSGADIRALSGRVPSLNIESSFGRTFPRFYI
                     RGLGNTDFDLNASQPVSLVYDDVVLENPVLKGFPVFDLNRVEVLRGPQGTLFGRNTPA
                     GIVKFDTVRPDAAGKNYVRLSYGSFGTINAEAGVGSGDASEVLSARVSVLYQHRDNWV
                     DNLDAPGDKDLEGFDDFAARLQVQAKPNDRLTLRTMVQLRMFEGSARLFRANAFAPGS
                     NKLIGLGGPATAFDREKTRADGLNFQSLTNINSAFTAEYDAGPVTITSVTSYWYGNLK
                     SRGDIDGGFGNVFAPSMGPGFIPFSAQSRDDVPSLDQFTQEVRISSNNTGGLGYQVGA
                     FYFSEKLDITSFDYGSPTDTAPAAIAGQRQTSEALGVFGSINYQFDSGLKLQAGARFN
                     HDNRTMVASRPLDTRPGFLGFGGPVAPQSAEVGDDVLTWDLSASFPLNEDVNLFARVA
                     KGYRAPSIQGRLLFGRTLSIADSETTMSY"
BASE COUNT          956 a         2230 c         2098 g          988 t
ORIGIN      
        1 ggcggtccag ttctccaccg ccgaggtcat cagcagctcg ttggggatca tgtacttgat
       61 ccggtcgcgc gtggtcacgg ctacggcgcg catcccgatc cggtccacct tgccgacggt
      121 cttgtcgctg ccgctgccca ccgcgatcac atcacccggc ttgatcgagc ggtccatcag
      181 caggatgatg cccgagatca ggttgcccag cgtcttttgc aggccgaaac cgatggcgag
      241 accaaaggca ccggaaaaca cggtcagcgc ggtcaggttg atgcccagcg cgtcgatccc
      301 gatcaggacc agcgcggtcc agacgaccac cccgaccagc ttttccccca gcacctgctg
      361 gcccagatcg agccggttca tgcggcggaa aaagcgccgg accagccggt tgagcagcca
      421 gcccgccacc agcaccagca cggccacggt gacgactttc agcccgccca gcagcgagat
      481 gtgatagccg ccaacgtcga gcgcgtttgt atcgagccag ccgaggaatt cgggccagct
      541 catgccgcgg cccaggcggc caggccgcgt tcgagatcgg cgatcaggtc ggccggctct
      601 tccagcccga tccacagccg caccgcgtgg cggttttccg gcgcgatccc gcgcggcggc
      661 cagggcatca cgtcgcggtc gcgctccgga tggctgccca gcgccaggct ttcaaaaccg
      721 ccccagctgt agccgatccc gaacaggctg agcgcgtcga tgaaggcatc gcgcgctttg
      781 aggtccttgc cggccatgat gatggtcatc agcccgcagc cgccggtaaa gtcgcgcttc
      841 cacagctcat gccccggcgc gccgggcagc atcgggaaca gcaccgcggc cacttccggg
      901 cggtcctgca gccactccgc tatttgcagc gcactggcgc aggagcgttc gagccgcacg
      961 cccatggtgc gcagaccccg cagcgccagc gcacaatcgt cgggtgcggc catctggccc
     1021 agcatcagcg agtgccgccg cagccgcggg taccattccg cattggcggt gactgcgccc
     1081 agcatcaggt ccgaatggcc gccgacatgc ttggtcagcg ccacgatcga caggtcgacc
     1141 ccgtgcttca gcgcctgaaa gccggtcggc ccggcccagg tgttgtcgag cagcaccacc
     1201 agcccgcgcg ccttcgcggc cgcagccagc gcgggcacat cgcacatctc catcgagacg
     1261 ctgccggggt tttccagcag caccgcgcgg gtcttgtcgg tgatcgcggc ggcaaagccg
     1321 tcaatatcgt agggatcgaa gtatcgcgcg gtgatgccgt aatcgcgcag gcccttctct
     1381 gcgaaagtcc ggctgggatc gtacgagttg tcggaaacca gcacctcgtc cccggtcttc
     1441 agcaccgcca gcagggccat gaccggcgcg gcgaggccgg tggggtagag catggttccg
     1501 gccgcgccgg gctccagcgc ggtcagggca tcggccagcg cccactgggt cggcccgccg
     1561 cgcgcgccat aatggaacac gccgtccttg ttgacgtgca gcccggcctg ctgcgccgcg
     1621 ctgtcctcat agaggtgcgt gctggcccgc cagaccggcg ggttgaccac cggtccggtc
     1681 cattcggggc gccgcccggc cgtgaccagc cgggtggcgg ggcccagatt gtcaggcgga
     1741 tcctttgcca tcggccctca ggccttgacc gccttcggcg tgtcgggatc gttgccccat
     1801 tcggtccagc tgccgtcata gagcgcggcg tcgtccttgc cggccaggtg cagcgcgaag
     1861 atcagcacgc aggcggtcac gccgctgccg cagctggtga tcaccggctt gcccatgtcg
     1921 accccggccg cttcgaacgt ggcgcggatt gccgccgggt ccttgaaggt gccgtcggcg
     1981 ttgtaaacat cggtgaaggg cacgttgaac gaacccggga tgtgcccgct ggcgatgttc
     2041 gggcgcggat cggcttcctc gcccgaccag cgcttggcgc cgcgggcatc gaccacctgt
     2101 tcgtccttgg agtggaggtt ggccaggacg tgggccttgg tccgcacgtt cttgtcatcc
     2161 tgccagacgg tgaagtggcg gtggcgcagg ctttccttgc cctgggtcag cgggcggccc
     2221 tcggccttcc acttggccag gccgccatcg aggatcgcca cgtcgtgcgc gccgaacatc
     2281 ttgagcatga accaggcgcg ggccgaggtc ttcaccgggc tgtcatcgta cagcacgatc
     2341 cggctgccat cgcccagacc cagcgactgc atgcggctgg cgaacttttc cgccggaggc
     2401 agggtgttct caaccggcgc gttggtgtcg accaggtcgc ccaggtccat gaacaccgcg
     2461 ccggggatgt gaccggcctc gtattcggcc tgagcatccc gcccggtgcc gggcagatgc
     2521 cgcgtgcaat ccacgatgcg caggtcgctc gcccccattt cgttggcgag ccattcggtc
     2581 gaaaccagac tttccatagt aatcgcgtcc tccaaaaggc cgtcctccac ggccattgcg
     2641 gcgccccttt tcgctgcaat tgcgaagaac ctagactcgc gcgggtgccg cgtcgagggg
     2701 ggaaatgctc aattgacggc gcgctggccc agccgggagt aggtgcgcgg ccccagatcg
     2761 agccggaacg gcttcagcgc gccgccgctg gcctcgggca gttcgcccac aacataggtc
     2821 tggacctgga ccagcgactc gcccgcggcc gtgcgcgccg cgacccgcaa gcgggccagc
     2881 ggcacgaaat aggcggcctc tccggcccgg atcggggtga ccgcggtcag cggcaggcgg
     2941 aagtcgccgg tgaattcggc gctttcgccg ggggccagtt cgaccagcgc gtggcgcggc
     3001 tccagccgct gcttgtcgtc cgcgatctgc gcctcgggcg gcagcgaggc gtgggccgaa
     3061 accatatcgc cttccaccgc cagcgcggtc agcgcctcgc taccggtgtt ggtcagccgc
     3121 agcacgtagt tgagcgtcgt cgccaccagc gaggcgttca tccgccgcgc ctccagcgtg
     3181 atcccgaggc cggcgggcag gcccaggccg aggtgcggct cgggcaggac ccgggccgga
     3241 gccgcgccca cagcggccgc gcccaccggg gccggagccg ccgccgcgcc aaccgggggc
     3301 agcggctgcg gcgcggccgc cgggacgggt tggggcacga tcggccgttc gaaagtgatt
     3361 tgcagcgggc gctcctcgcg ccggcggctc caccacaggc cggcaaagac cagcgccagc
     3421 agcgcggcca gcccggcgat ccagggccag taccagcccg ccgctgccgc ggccgccgcc
     3481 ggtgcggcgg tgctggcggg cgggggcacc ggggcaaagc cggtcgggaa agcgcccggc
     3541 gcggccggtc cggtggcggg agcggtcgcg ggcggcaagg ccgtgccagc gggcgctgcc
     3601 gtggccgggg cgggagtcgc cgccgccgga gcgccggcct gacgcggggc actggcggcc
     3661 gggcgctgcg cggcgccacg ggcgggcgtt gctgtcggtg aaggcgccga ggcagtcggg
     3721 gtctgggcgg cgctggggct tgggctgggc gctgcgggcg tcgcgctctg ggtcgggcgc
     3781 gggaccgcct tcgtcaccac cggggcgtcg cgatcgaccg ggccctgggc cttcggaccc
     3841 gcgggctgca cggtcgcgcc gggcagctgc cagccgcccg attgggccag ccccggcgca
     3901 ggggcaagcg ccagcatcag cgccacagcg ctggctgcgg tcaagcggcc cccggtcttg
     3961 ttcatcgtgt tgtccatttc agcgcggatg cggcgcgcta gcgcctctgg cactgaatag
     4021 gaagtgaacc ctgtcattcc aaccgccgac ttgtgcattg cgcgcgatag aggcatggcg
     4081 cgttgcatgg cagacacacc ttctcccctg catctgggcc agcaaagcgc gcttccggct
     4141 tctcccgaag cggcggtgct ggactatgtc cccaatccgc gcccgggcgc gctgttcctg
     4201 gtgcgcttcg ccgcaccgga attcacctcg ctgtgcccgg ttaccggcca acccgatttc
     4261 gcgcacctgg tgatcgatta cgccccgggc gagacgatcg tcgaatcgaa aagcctgaag
     4321 ctgttcctgg gcagctttcg caaccacgcc ggctttcacg aggacgtgac cgtgggcatc
     4381 ggccagcgcc tgtttgccga aatgaagccc cagtggctgc ggatcggcgg ctactggtat
     4441 ccgcgcggcg gcattccgat cgacgtgttc tggcaaagcg gcgccgcgcc ggacggcctg
     4501 tgggtgccgg atcagggcgt cgcgggctac cgcgggcgcg gctgatcggc cacaccgggc
     4561 gtgtaatatt tcggtcacac gcatttcgct gtttcttcgt taaccctcgc tcgctagcgg
     4621 gcgcggcatc ccattcccta acgaaggacc gctcccgcga tgcgtttcac gtcgaccctc
     4681 cgtaccgcca ccgcctcgtt cgccctggct gcctcgctgg ccactgccgc tccggccctg
     4741 gcccaggaca acgccgaaga cggcgaaggc ctgtccgaaa tcatcgtcac ggcccagcgc
     4801 cgcgaggaaa accagaagga cgtgccgatc tcggtcgcca ccgtgcgcgg cgatgcgctg
     4861 gccgcggtca gcggttcggg cgccgatatc cgcgccctgt cgggccgggt tcccagcctc
     4921 aacatcgaaa gctcgttcgg ccggaccttc ccgcgcttct acattcgcgg ccttggcaac
     4981 accgatttcg atctcaacgc ctcgcagccg gtcagcctgg tctatgacga cgtcgtcctc
     5041 gaaaacccgg tgctcaaggg cttcccggtg ttcgatctca accgcgtcga agtgctgcgc
     5101 gggccgcagg gcaccctgtt cggccgcaac accccggccg ggatcgtcaa gttcgacacc
     5161 gtccgtccgg atgctgccgg caagaactat gtccggctga gctacggcag cttcggcacg
     5221 atcaacgccg aagcaggcgt gggcagcggc gatgccagcg aagtgctgtc ggcccgcgtc
     5281 tcggtcctct accagcaccg cgacaactgg gtcgataacc tcgacgcccc gggcgacaag
     5341 gacctggaag ggttcgacga tttcgccgcc cgcctgcaag tccaggccaa gccgaacgac
     5401 cggctgaccc tgcgcaccat ggtccagctg cgcatgttcg aaggttcggc ccgcctgttc
     5461 cgcgccaacg cctttgctcc gggcagcaac aagctgatcg gcctgggcgg cccggcgacc
     5521 gcgttcgacc gcgaaaagac ccgcgccgac ggcctcaact tccagagcct gaccaacatc
     5581 aattcggcct tcaccgccga atacgatgcc ggcccggtca cgatcacgtc ggtcaccagc
     5641 tactggtacg gcaacctcaa gagccgcggc gatatcgacg gcggcttcgg caacgtcttt
     5701 gccccttcga tgggcccggg cttcatcccg ttctcggccc agtcgcgcga tgacgtgccg
     5761 agcctggacc agttcacgca ggaagtgcgg atctcctcga acaacaccgg cggcctcggc
     5821 tatcaggtgg gcgcgttcta cttcagcgaa aagctggaca tcaccagctt cgactatggc
     5881 agcccgaccg acaccgcccc cgcggcgatc gccggccagc gccagaccag cgaagcgctg
     5941 ggcgtgttcg gatcgatcaa ctaccagttc gattcgggcc tgaagctgca ggccggggcg
     6001 cgtttcaacc acgacaaccg caccatggtc gcctcgcgtc cgctggatac ccgtccgggc
     6061 ttcctcggct ttggcggccc ggtcgctccg cagagcgcgg aagtgggcga tgacgtgctg
     6121 acctgggacc tctcggccag cttcccgctg aacgaagacg tcaacctgtt cgcccgcgtg
     6181 gccaagggct atcgcgcgcc gtcgatccag ggccgcctgc tgttcgggcg caccctttcg
     6241 atcgccgaca gcgaaaccac catgagctat ga
//
LOCUS       DASUDV010000379         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1227667,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000379 DASUDV010000000
VERSION     DASUDV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227667"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VFV30_08250"
     CDS             <1..142
                     /locus_tag="VFV30_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX4848121.1"
                     /translation="FEGDKASEAARYPLGNRIREIVEASDGSLYVLEDGPNARLLRLS
                     RK"
     gene            202..720
                     /locus_tag="VFV30_08255"
     CDS             202..720
                     /locus_tag="VFV30_08255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4848122.1"
                     /translation="MTEALTLIPLDAVDPALVEQLLDRAFEPERRQRTAYKLREGVDW
                     LPGLSFAAVDGEQHLVGTIQCWPVALTDPQGRAHPLILVGPVAVLPEAQGAGIGKALM
                     TAAIAALDPRGMLPQVMIGDPEYYGRFWGFAAQPTAGWTLPGPWEPRRLLVRCDNPAV
                     LPKEGALGPWRG"
     gene            744..1283
                     /locus_tag="VFV30_08260"
     CDS             744..1283
                     /locus_tag="VFV30_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX4848123.1"
                     /translation="MPYVPPPELASLSLAEIAALNAQRKLPPVDQWRPAAVGDSLMVI
                     KADGRWFHDGGEIRRPAMVRAFAGLLWRDGEGQHWLVTPHEKQAIAVEDAAFIATDVV
                     QQDDALAFRLNTDEVIVAGPEHPLIARGEADCPALYLGVRHGTEARINRSTWLQLVDL
                     AGEDLSVSSQGTRFSLVPR"
     gene            1280..1858
                     /locus_tag="VFV30_08265"
     CDS             1280..1858
                     /locus_tag="VFV30_08265"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX4848124.1"
                     /translation="MSLVKRLARLHEEGHARPIDHRDESDFAERELRPAAVLAAITER
                     DRPGFLLIHRPSNMRSHPGQVAFPGGKIDPGETPEEAALREAWEELGIHQRDVTVIGT
                     SDTYRTGSGYAITPVIAVVPPDIELNPSPTEVAAWFEAPVDFVFDQENHVRHSGFWQG
                     RTRSYIEILWQEHRIWGVTAGIIANLAKRIAW"
     gene            1852..>2360
                     /locus_tag="VFV30_08270"
     CDS             1852..>2360
                     /locus_tag="VFV30_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX4848125.1"
                     /translation="MVTRLPRADWLERADLSVLVKALGPGQARYVGGAVRDTLLGLAV
                     KDVDIATPLEPQEVMRRLKAHSISVIPTGIEHGTVTAVLPGGPVEITTLRRDVSTDGR
                     RATVAFATNWQDDAARRDFTINALYADPETGEVFDWFGGLDDLGDRRVRFIGDARQRI
                     REDHLRILR"
BASE COUNT          393 a          796 c          793 g          378 t
ORIGIN      
        1 ctttgaaggc gacaaggcca gcgaagcagc ccgctatcca ctgggcaacc gcatccgtga
       61 aatcgtcgaa gccagcgacg gctcgctcta tgtgctcgag gatgggccga acgcgcggct
      121 gctcaggctt tcgcgcaaat aaccgcacaa gcaggcgcac aagtagacaa ggcgtcccgc
      181 agcccctatc ggcgcgcctc gatgaccgag gccctcaccc tgatcccgct cgacgcggtc
      241 gatcccgcgc tggtcgagca attgctcgac cgggccttcg agcccgagcg gcgccagcgc
      301 acggcctaca agctgcgcga aggggtggat tggctgcccg gcctgtcgtt cgcggcggtc
      361 gatggcgaac agcacctggt cggcacgatc cagtgctggc cggtggcgct gaccgatccg
      421 cagggccgcg cccatccgct gatcctggtc ggcccggtgg cggtgctgcc cgaggcgcaa
      481 ggggccggga tcggcaaggc gctgatgacc gctgccatcg ccgcgctcga tccgcgcgga
      541 atgctgccgc aggtgatgat cggcgatccc gaatactacg gccggttctg gggctttgcc
      601 gctcagccca ccgccggctg gaccctgccc ggcccgtggg aaccgcgccg gctgctggtg
      661 cgctgcgata atccggcggt cctgcccaag gaaggcgcgc tggggccttg gcgcggatag
      721 ggcaatctgg catcgctgcc gccatgccct atgttccgcc gcccgaactt gccagcctga
      781 gccttgccga gatcgcggcg ctcaacgccc agcgcaagct gccgccggtc gatcaatggc
      841 gccccgcagc ggtgggtgac agcctgatgg tgatcaaggc cgatggccgc tggttccacg
      901 atggcggcga aatccggcgc ccggcaatgg tgcgcgcctt cgccggcctg ctgtggcgcg
      961 atggcgaagg ccaacactgg ctggtcaccc cgcacgaaaa gcaggcgatc gcggtcgagg
     1021 atgcggcctt catcgccacc gatgtggtgc agcaggacga tgccctggcc tttcgcctca
     1081 acaccgacga ggtgatcgtt gccgggccgg aacaccccct gatcgcccgc ggcgaagctg
     1141 attgtcccgc gctctacctt ggtgtccgcc acggaaccga ggcccggatc aaccgttcga
     1201 cttggctgca actggtcgat ctggcgggcg aggatctgag cgtttccagc cagggaaccc
     1261 ggttttcgct ggtgccgcga tgagccttgt caagcgcctt gcgcggctgc acgaggaagg
     1321 ccacgcccgg ccgatcgatc accgcgacga aagcgatttt gccgagcgcg agcttaggcc
     1381 cgcggcggtg ctggccgcga ttaccgaacg cgatcggccc gggttcctgc tgattcaccg
     1441 tccctcgaac atgcggtcgc accccggtca ggtcgccttt ccgggcggca agatcgatcc
     1501 gggtgagacg cccgaagaag ccgccctgcg cgaagcctgg gaagaactgg gcatccacca
     1561 gcgcgacgtg accgtgatcg gcaccagcga cacctaccgc accggatcgg gctatgcgat
     1621 caccccggtc atcgcggtgg tcccgcccga tatcgagctc aaccccagcc cgaccgaggt
     1681 tgccgcctgg ttcgaagccc cagtggactt cgtgttcgat caggaaaacc acgtgcggca
     1741 ttcaggattt tggcaaggcc ggacaagaag ctatatcgag atcctatggc aggaacaccg
     1801 gatctggggc gtgaccgcgg ggatcatcgc caacctggcg aaaaggatcg cgtggtgaca
     1861 aggctgcccc gggccgactg gctggagcgc gccgacctgt cggtgctggt caaggcgctt
     1921 ggccccggcc aggcccgcta tgtcggcggc gcggtgcgcg acaccttgct ggggctggcg
     1981 gtgaaggacg tcgatatcgc caccccgctt gagccgcagg aggtgatgcg gcggctcaag
     2041 gcccattcga tatccgtgat cccgaccggg atcgagcatg gcaccgtcac cgcggtgctg
     2101 cccggcggac cggtggagat caccaccctg cgccgcgatg tttcgaccga tggccgccgc
     2161 gcgacggttg cctttgccac caactggcag gacgatgccg cgcgccgcga tttcaccatc
     2221 aatgcgctct acgccgatcc ggaaaccggt gaagttttcg actggttcgg cggactggac
     2281 gatctggggg atcgccgggt gcggttcatc ggcgatgccc gccagcgaat tcgcgaagac
     2341 cacttgcgga tcctgcgcta
//
LOCUS       DASUDV010000380         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_719692,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000380 DASUDV010000000
VERSION     DASUDV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719692"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFV30_08275"
     CDS             complement(<1..1230)
                     /locus_tag="VFV30_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848126.1"
                     /translation="MALDRADGIAAEAELAKALRQGAPRNAIAAPMGEALLIQGRNAR
                     AREWLGGGTFAKGDEAYGWRMLGLLERREGNLAAAGKAYDRALGFTPDDPRLWVDIGR
                     LRYVGGEHLLAIEASERALAKGGEDIRALEFRAELLRDSQGWAVALPLYAQAVDLAPN
                     DLGLLAGYAATLGEAGQLRQMLAVTRKMLAIAPRNPQAWYLQAVLAARAGKVDLARAM
                     LNRAGQRLKSVPAAQLLEAALELEAGNAEVAVGLLEELDRDQPANHRVQMLLARAYLD
                     AGANDALFGRFEIALRRDDTPPYLLAIMARAHEQRGDRIAAAALLDRVAAARAVPLAG
                     IVPSARPGDAAAQWNANPAVSGNAVALVRQLLSRGEIEPAGRVAERMQQLRPGSVDAL
                     GLAGDLDLVRANPASAID"
     gene            complement(1334..>2681)
                     /gene="prsR"
                     /locus_tag="VFV30_08280"
     CDS             complement(1334..>2681)
                     /gene="prsR"
                     /locus_tag="VFV30_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX4848127.1"
                     /translation="KLLIVEDDAGLQAQLKWAWPDFEVVIAGDRASAITALRSEEPAV
                     VTLDLGLPPDPDGTTEGFAVLDEIMAIKPDTKVIVASGHGARESALQAIAKGAYDFYQ
                     KPVDIEALGLIVRRALQLHRIETENRQLASRAGDDNRVLGRMITAAPEMVKVARTIER
                     VANTNVSVMLLGASGTGKELLARGLHEASDRRNGAFVAINCAAIPENLLESELFGHEK
                     GAFTGAVKTTEGKIEMANGGTLFLDEVGDIPLPLQVKLLRFLQERVIERVGSRRSIPV
                     DTRIVCATHQDLEAMIGDGRFREDLFYRLAEIVVKIPSLAERPGDAALLAKAFLARFA
                     KEMNPAVKGFAPDALAAIDGWGWPGNVRELENRVKRAVIMTEGKLVGAADLDLGEGEE
                     SEGPVLNLKSAREATDRKVIRHALARSEGNISNTAKLLGISRPTLYDLLKQYDLQV"
BASE COUNT          447 a          917 c          856 g          461 t
ORIGIN      
        1 gtcaatcgca ctggcgggat tggcgcgcac cagatcgaga tctccggcga ggccaagcgc
       61 atcgaccgac ccgggtcgaa gttgctgcat ccgctctgcg acccgccccg ccggttcgat
      121 ctctccgcgc gagagcagct ggcgaaccaa tgcaaccgca ttgcccgaaa ctgccggatt
      181 tgcattccat tgtgcggcgg cgtcgccggg ccgcgcggat ggaacaattc cggcaagcgg
      241 cactgctcgg gctgccgcga cacggtccag caaggctgcc gccgcgatcc gatcgccgcg
      301 ctgttcgtgg gcgcgtgcca tgatcgccag gagatagggc ggcgtatcat cgcggcgcag
      361 cgcgatttcg aacctgccga aaagtgcatc gttggcgccg gcatcgagat aggcccgggc
      421 cagaagcatc tgcacccggt gattggcggg ctgatcgcgg tccagttcct cgagcaggcc
      481 caccgcgact tcggcatttc cggcctcaag ctcgagcgcg gcttcaagca gctgcgcggc
      541 gggaaccgat ttcagtcgct gcccggcacg gttaagcatg gcgcgggcaa gatctacctt
      601 gccggcgcgg gccgccagta ccgcctggag ataccaggcc tgcggattgc gcggcgcgat
      661 cgccagcatc ttgcgggtaa ccgccagcat ctgccggagc tgccccgctt cacccagggt
      721 cgcggcatag ccggccagca agccaagatc gttcggggca aggtctaccg cctgggcata
      781 gagcggcaag gccactgccc aaccttgcga atcgcgcagc agttccgcgc gaaattccag
      841 cgcgcggatg tcctccccac ccttcgcaag cgcccgctcc gaggcttcga tcgccagcag
      901 gtgctcgccg ccgacatagc gcaggcgccc gatgtcgacc cacaggcgcg gatcatcggg
      961 cgtaaagcca agcgcgcgat cataggcctt gccggccgcg gcaagattgc cttcgcgccg
     1021 ctcgagcagg ccgagcatcc gccagccata ggcttcatca cccttggcga atgtaccgcc
     1081 ccccagccat tcgcgggccc gggcgttgcg cccctggatc aacagcgcct cgcccatcgg
     1141 tgcagcgatt gcgttgcgcg gcgcgccttg gcgcagtgcc ttagcgagtt cggcctcagc
     1201 ggcaatccca tcggcgcggt caagcgccac gcgcgccttt gcgatccgtt cggacggacc
     1261 ttcaggcgcc gcaccaagcg gcgcggcaag aatcaaggcc gtaacgagaa gtgcgaaggc
     1321 ccgccgcatc tgctcagacc tgcaggtcgt attgcttcag cagatcatac agggtcggac
     1381 ggctgatccc gagcagcttg gcggtattgg aaatgttgcc ttcgctgcgg gccagagcat
     1441 ggcggatcac cttgcggtcg gtggcttcgc gcgctgactt gaggttgagg accgggccct
     1501 cgctttcctc gccttcgcca agatcgagat cggcggcgcc aaccagcttg ccctcggtca
     1561 tgatgaccgc ccgcttgacc cggttttcca gctcgcgaac gttgcccggc caaccccagc
     1621 catcgattgc ggccagcgca tcgggcgcaa atcccttgac cgccgggttc atctccttgg
     1681 cgaaacgcgc aaggaacgcc ttggccagca gcgccgcatc gccaggccgt tcggccaggc
     1741 ttgggatctt caccacgatt tcggccaggc gatagaacag gtcttcgcga aagcgcccgt
     1801 cgccgatcat ggcttcgagg tcctggtggg tggcgcagac aatccgggta tcgacaggga
     1861 tcgatcgccg cgagccgacc cgctcgatca cgcgttcctg cagaaagcgc agcagcttga
     1921 cctggagcgg tagcggaatg tcgcccactt catcgaggaa cagcgttccg ccgttggcca
     1981 tttcgatctt gccttcggtg gtcttcacgg cgccggtgaa ggcgcccttc tcatgcccga
     2041 acagctcgct ttcgagcagg ttctcgggaa ttgccgcgca gttgatcgca acgaacgcgc
     2101 cattgcgacg gtcgctggcc tcgtgcaggc cgcgggcgag cagttccttg ccggtgccgc
     2161 tggcacccag cagcatgacc gaaacattgg tgttggccac ccgttcgatc gtgcgggcaa
     2221 ccttgaccat ttccggcgcg gcggtgatca tccggccaag cacccggtta tcgtcccccg
     2281 cgcgcgaggc cagttgccgg ttttcggttt cgatccggtg cagctgcaag gcgcggcgca
     2341 cgatcagccc caacgcctcg atgtcgaccg gcttctggta gaagtcatag gcacccttgg
     2401 cgatcgcctg cagcgcgctt tcccgggcgc cgtggcccga ggcgacgatc accttggtat
     2461 cgggcttgat cgccatgatc tcgtccagca ccgcaaagcc ctcggtcgtg ccgtcgggat
     2521 cgggcggaag gccaaggtcg agcgtgacca ccgcgggctc ctcggaacgc agcgcggtga
     2581 tcgcgctggc gcggtcaccg gcgatcacca cctcgaaatc gggccaggcc catttgagct
     2641 gggcctgcag tccggcatcg tcctcgacga tcagcagttt g
//
LOCUS       DASUDV010000381         7252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_631362,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000381 DASUDV010000000
VERSION     DASUDV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631362"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..813
                     /gene="rplJ"
                     /locus_tag="VFV30_08285"
     CDS             301..813
                     /gene="rplJ"
                     /locus_tag="VFV30_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX4848128.1"
                     /translation="MDRSQKAESVASLNAVFNEVGVVVITRNLGMSVAQSTDLRNKIR
                     DAGASYKVAKNRLAKLAIKDTSYEGIGELLTGPTAIAASVDPVAAAKAVVDFAKTNDK
                     IEIVGGSMGSQVLTPEGVKALASMPSLDQLRATLIGLVQAPATKIAQLSTAPAAKLAR
                     VFGAYAKEAA"
     gene            874..1251
                     /gene="rplL"
                     /locus_tag="VFV30_08290"
     CDS             874..1251
                     /gene="rplL"
                     /locus_tag="VFV30_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX4848129.1"
                     /translation="MADIAKLVEELSKLTVLEAADLAKALEEAWGVSAAAAVAVAAPA
                     GGGEAAAAVEEKTEFDVILTGDGGNKIAVIKEVRAITSLGLTEAKALVEAAPKAIKEG
                     VNKAEAEDIKAKIEAAGGTVEVK"
     gene            1355..1915
                     /locus_tag="VFV30_08295"
     CDS             1355..1915
                     /locus_tag="VFV30_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848130.1"
                     /translation="MVPTLETDRLILRPLTLDDEAAIQRIFPQWEIVRWLDAKVPWPY
                     PDDGARFFLEQVALPQMARGEGWHWSIRRRAEPAELIGEITLQESDLTNRGFWIDPEW
                     HGRGFASEAADAVTAFWFGTLDRPVLRIAKAAGNIASRRISQKQGMRMVRQFEGELVS
                     GPCPFELWELTREEWRGQTGREGHSG"
     gene            1925..2704
                     /locus_tag="VFV30_08300"
     CDS             1925..2704
                     /locus_tag="VFV30_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848131.1"
                     /translation="MTAVPLPPCVTLWIGDRLGPLERACLRSIMRQGHRLALYCYDPV
                     AGVPEGVELRDAAGIFPREAIVRHKSGSPSLFSNRFRYELQRRGLGIWVDTDVYLLSP
                     LDDPAPTLFGREQEGNIAIGILRLPPDSPLLAPLLALFEQPYVPPWIGLRPRIAAHAR
                     ALLRGRVELGDLPWGVAGPRAVTALAARHGLAGLALPPEVFYPWSWREAHWIFDPARS
                     LDAWLTPQTRALHFYNYLLGAAKDAPAAPGSFMARLQSEGA"
     gene            complement(2823..3254)
                     /locus_tag="VFV30_08305"
     CDS             complement(2823..3254)
                     /locus_tag="VFV30_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4848132.1"
                     /translation="MRLAVPSLAATALALALTACGSPPEDKAAPAGGEASPAATTAAA
                     TTGGPPAAFGQCAACHAVEPGKHGVGPSLAGVFGTKAGDIAGYAFSDAMKASNLTWDE
                     KTLDAYLTNPMKMVPGTKMTYAGMADAEKRKAVIDYMKTLK"
     gene            3469..4863
                     /locus_tag="VFV30_08310"
     CDS             3469..4863
                     /locus_tag="VFV30_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX4848133.1"
                     /translation="MAEPHALTPWRSELRETLRLALPLAATNLLQMLIHAVDVIFIAR
                     LGDQPLAASSLGISIFGLTMWGLTGLTGACAPLIAAELGRRSHAVREVRRTVRMALWM
                     AVGFGILGMAIGFAGEPLLLLSGQDPEVAALSGQFLAILSWAMIPMIMASVFRIFVAA
                     LGKPGYATAITLLALGTNILGNWVLVFGNLGAPALGLTGSALSSLLTSLAMLAAYVVV
                     IGRDRRLRRYRIFGRWWRPEWQRLREMLKIGTPIALTVLAEAGLFSAAALLMGRIGET
                     ELAAHTLALNLAALAFQIPFGIAQAATIRVGYHFGACDPAAAGRAGWSALGLSVGFMG
                     TTAAAMLLAPHFILRLYGDPDAPQSAALTALAVQYLAVAAAFQLFDGAQAVAAGALRG
                     LQDTRMPLVIALFGYWAVGFVTAIGLGFYTPLQGIGVWIGLAVGLVVVAALLTWRWHR
                     RERLGLLPEAVPAA"
     gene            4873..5190
                     /locus_tag="VFV30_08315"
     CDS             4873..5190
                     /locus_tag="VFV30_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848134.1"
                     /translation="MADDDILHRPMVLDEFLPLVGRQFLANCDPAEVELTLVEASPLK
                     DRGVTDRPPFILVFHTPPEAMLVEGSYVMRCGGWGPDRISIWPTMPPRPAEPGYYYQA
                     VFN"
     gene            complement(5204..5791)
                     /locus_tag="VFV30_08320"
     CDS             complement(5204..5791)
                     /locus_tag="VFV30_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX4848135.1"
                     /translation="MSNLYLGQIIQGGWNYAPRGTALCNGQSLGIAQNSALFALLGVS
                     FGGNGTTTFNLPDLRSRSMMHWGQGNGLSPVQLGQVGGAESVTLNSGQLPTHTHAATF
                     ASTSSLNASTAKATAQTPAPGLVLAKSNDNTGTAIPFIYAPAGTVADQALAGLNVAGS
                     VTVAPAGNSLPVPTISPYQAVTHVIALEGIFPSRS"
     gene            complement(5979..7187)
                     /locus_tag="VFV30_08325"
     CDS             complement(5979..7187)
                     /locus_tag="VFV30_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848136.1"
                     /translation="MTLPQPLPAMALGLPASLGALVRLLLERPDLQDQLGAILAADAF
                     AAEALAVAAEHGVACDAEALRAVLRPDPLGLGRFAAAPVALDGWPPPGWLPTRSVPTG
                     DAPAFDWLWFGSRRLDAPFFEDEVRRASALPLNWLLRIRTGLRDLVKGAPAEPEPPLS
                     GLVFHLSRCGSTLLAQMLGALPGHTVSSEPEPLDAVLRWTAAARPDPAESSAAIRAMV
                     AALGRPRHPAPRHFIKLEVWHTLFLPQLRAAFPAVNWVYLHRDPAEVLVSLAAQPSMH
                     ILPGALDPALLGIDPAQAEGPDDHAAMVLHAAMRAVIDHWPLGGGLAVDYPEIPAAAT
                     GAIAAHFGLELDSAARARMAAAAQRDAKAPERPFAADRAAKQAEATDSIRRAAAQWLD
                     PLRQQLAQKR"
BASE COUNT         1091 a         2421 c         2533 g         1207 t
ORIGIN      
        1 agccgcgccg ccgcgccggg acttgacttt gccgggggtc tgggccaatg gcccgccccg
       61 accgggcgtt cgcgtccgtt catccgtccg agacagctgc tgaggcttcc cctcttaatt
      121 cgcagcctag acggggacag agcaaattcc ggcccgcgca attgcccggg cccggcctgg
      181 cgtttcccgc gccctccttc cccatcgcac ggactcgccc atggtgcgca agcgccatgg
      241 acaaacgtag ccggccgttc ggggcatgcc cccttgcggt catttgtgaa ggagtaaggc
      301 atggatcgtt cgcagaaagc cgaatcggtc gcatcgctga acgcggtctt caacgaagtt
      361 ggcgtggtgg tcatcacccg caacctcggc atgtcggtgg cccagtccac cgacctgcgc
      421 aacaagatcc gcgacgccgg tgcgtcctac aaggttgcga agaaccgtct tgccaagctt
      481 gccatcaagg acacgtccta cgagggtatc ggtgaactgc tcaccggccc caccgcgatc
      541 gccgcttcgg tcgatccggt tgccgctgcc aaggcggtcg tggatttcgc caagacgaac
      601 gacaagatcg aaatcgtcgg cggatcgatg ggttcgcagg ttctgacccc cgaaggtgtc
      661 aaggcgctcg cctcgatgcc ctcgctggat cagctgcgcg cgaccctcat cggactggtc
      721 caggctccgg ccacgaagat cgcccagctg tcgaccgctc cggcggccaa gctggcccgc
      781 gtcttcggtg cctacgccaa ggaagccgca taagaccgtt caacgaattc cccgggcgcg
      841 atgcccgggt ccatcaatca ggagtgaaac atcatggccg atatcgccaa gcttgttgaa
      901 gaactttcga agctgaccgt cctggaagcc gccgacctcg ccaaggctct cgaagaagcc
      961 tggggcgtga gcgctgctgc cgccgttgcc gttgctgccc ccgccggtgg cggtgaagcc
     1021 gctgccgcgg ttgaagaaaa gaccgaattc gacgtcatcc tgaccggtga cggcggcaac
     1081 aagatcgccg tcatcaagga agtccgcgcg atcacctcgc tcggcctgac cgaagccaag
     1141 gcgctggtgg aagccgctcc gaaggcgatc aaggaaggcg tgaacaaggc tgaagccgaa
     1201 gacatcaagg ccaagatcga agccgctggc ggcacggtcg aagtcaagta agcttcgctt
     1261 cgctgggttc agcgaaacag gaaagggcgg ctcccgcggg ggccgccctt tttgtttgcc
     1321 ttgcaatggt ccggctaccg ggcaaactcg ggccatggtt ccgacccttg agacagaccg
     1381 gctgatcctg cgcccgctga cactggacga tgaagcggca atccagcgga tatttccgca
     1441 atgggagatc gtccgctggc tggatgccaa ggtgccctgg ccctacccgg atgacggcgc
     1501 ccgcttcttc ctcgagcagg ttgccttgcc gcagatggcc cggggcgagg gctggcattg
     1561 gagcattcgc cgccgggcgg aaccagccga gctgatcggc gaaatcacgc tgcaggaatc
     1621 ggatcttacc aaccgcggct tctggatcga tccggagtgg cacggccgcg gttttgccag
     1681 cgaagcggcc gatgcggtga ccgcgttctg gttcggcacg ctggaccggc cggtgctgcg
     1741 gattgccaag gcggccggca acatcgcgtc gcgccggatt tcgcaaaagc agggtatgcg
     1801 catggtccgg cagttcgagg gcgaactggt cagcgggccg tgcccctttg agctgtggga
     1861 gctgacccgt gaggaatggc gcgggcaaac cggccgggaa ggacacagcg gctagctgcc
     1921 cgccatgacc gccgttccgc ttccgccttg cgtcacgctc tggatcggcg accggctcgg
     1981 gcctttggag cgggcctgtc tgcgctcgat catgcgccag gggcatcgcc ttgcgctcta
     2041 ctgctacgat ccggtcgcgg gggtgcccga aggggtcgaa ctgcgcgacg cggccggtat
     2101 cttcccgcgc gaggcgatcg tgcgccacaa gagcggcagc ccctcgctgt tctccaaccg
     2161 gttccgctat gaattgcagc gccgcgggct tggcatctgg gttgataccg atgtctacct
     2221 gctgagcccg ctggacgatc cggcgccgac cctgttcggg cgcgagcagg agggcaacat
     2281 cgcgatcggg atcctgcggc tgccgccgga ttccccgctg ctggccccgc tgctggcctt
     2341 gttcgagcag ccctatgttc ccccctggat cgggctgcgc ccccggatcg cggcccatgc
     2401 ccgggcgctg ctgcgcgggc gggtggagct gggcgacctg ccctggggcg tggccggccc
     2461 gcgcgcggtg accgcgctgg ccgcgcgcca cggcctggca ggcctcgctc tgccgccgga
     2521 ggtgttctat ccctggtcgt ggcgcgaggc gcactggatc ttcgatccgg cccggtcgct
     2581 ggacgcgtgg ctgaccccgc agacccgcgc gctgcatttc tacaattacc tgctgggcgc
     2641 ggcgaaggac gcaccggcgg cgccgggcag cttcatggcg cggctgcaaa gcgaaggggc
     2701 ctgacggccg ggtccgtgac agcaaaaacg ctgtagttgc aaatgcaaag gggccggagc
     2761 tttcgctccg accccaggcg cgacccgaag ttctcgttgt tatatcttcg ggtataggga
     2821 cgctatttga gagttttcat atagtcaatc acggccttcc gcttctccgc atcggccatt
     2881 ccggcgtagg tcatcttggt ccccgggacc atcttcatcg gattggtcag ataggcgtcc
     2941 agggtctttt catcccaggt caggttcgat gccttcatcg catcggaaaa ggcatagccg
     3001 gcaatgtcgc ccgccttggt gccgaagact ccggccaggc tggggccgac gccgtgcttg
     3061 cccggttcaa ccgcgtggca ggcggcgcat tggccaaagg cggccggcgg accgccggtg
     3121 gtcgcggccg cggtcgtggc ggccggcgag gcttcgcccc cggccggcgc ggccttgtct
     3181 tcgggcgggg atccgcaggc ggtcagggcc agggcaaggg cggtcgcggc aagcgacgga
     3241 acggcaaggc gcatcggttc gggttccttc aggcagcgaa gcggcgcgtc tcccccagac
     3301 ggcaaccgcg gttgtaaccg ccatggttgt gccgcgtttg cccggccgcg tgaagtgtct
     3361 ttgttgcagc aggcgttgca cgaagcgcac ggtcgcgcgg caaagcgcag ggcggcctgc
     3421 gcttgggctt tgccatggcc cggccaaggc cctatccgga ccggcttcat ggccgaacca
     3481 cacgccttga ccccctggcg cagcgagctg cgcgaaacgc tgcggctggc cttgccgctg
     3541 gcggcgacca acctgttgca gatgctgatc cacgcggtcg acgtgatctt cattgcccgg
     3601 ctgggcgacc agccgctggc cgcatccagc ctcgggatct cgatcttcgg cctcaccatg
     3661 tgggggctca ccggcctgac cggggcctgc gccccgctga tcgcggccga gctgggccgg
     3721 cgatcccacg cggtgcgcga agtgcggcgc acggtgcgca tggcgctgtg gatggcggtg
     3781 ggctttggca tcctcggcat ggcgatcggc tttgccggag agcccttgct gctgctgtcc
     3841 gggcaggatc ccgaagtggc ggcgctgtcg ggccagttcc tggcgatcct gtcgtgggcg
     3901 atgatcccga tgatcatggc cagcgtgttc cggatcttcg tcgccgcgct cggcaagccc
     3961 ggctatgcca cggcgatcac cctgctggcg ctcggcacca acatcctggg caactgggtg
     4021 ctggtgttcg gcaacctggg cgcgccggcg ctggggctga ccggctcggc cctgtccagc
     4081 ctgctgacct cgctcgccat gctggcggcc tatgtcgtgg tgattgggcg tgaccggcgg
     4141 ctgcggcggt accggatctt cgggcgctgg tggcggccgg aatggcagcg gctgcgcgaa
     4201 atgctcaaga tcggcacccc gatcgcgctg accgtgctgg ccgaggccgg cctgttcagc
     4261 gccgccgcgc tgctgatggg ccggattggc gagaccgaac tggccgccca caccctggcg
     4321 ctcaatctgg cggcgctggc cttccagatc ccctttggca ttgcccaggc ggcgacgatc
     4381 cgggtcggct accacttcgg cgcgtgcgat cccgccgcgg ccgggcgtgc cgggtggagc
     4441 gcgctgggcc tctccgtcgg cttcatgggc acgacggcgg cggccatgct gctggccccg
     4501 cacttcatcc tgcggctcta tggcgatccc gacgcgccgc aaagcgcggc gctgaccgcg
     4561 ctggcggtgc agtacctggc cgtggcggcg gcgttccagc tgttcgacgg ggcccaggcc
     4621 gtggccgccg gggcgctgcg cgggctgcag gacacgcgga tgccgctggt gatcgcgctg
     4681 ttcggctatt gggcggtcgg ctttgtcacg gcgatcggtc tgggcttcta cacgccgctg
     4741 cagggcatcg gggtgtggat cgggctggcg gtcggtctgg tggtggtggc ggccctgttg
     4801 acctggcgct ggcaccggcg ggaacgactg ggactcttgc cagaggccgt cccggcggct
     4861 taggctgctg ccatggcaga cgacgacatc ctccaccgcc cgatggttct tgacgaattc
     4921 ctgccgctgg tcggccggca atttctggcc aattgcgatc ccgcggaagt ggagctgacc
     4981 ctggtcgaag cctcgccgct caaggaccgg ggggtcaccg atcggccccc gttcattctg
     5041 gtctttcaca ccccgcccga ggccatgctg gtcgaaggca gctatgtgat gcgctgcggg
     5101 ggctggggac cagaccggat ttcgatctgg cccaccatgc ccccgcgtcc agctgaaccg
     5161 gggtattact accaggccgt gttcaactga tcgcgccgcc tgcctagctg cgggacggga
     5221 agatgccttc cagcgcgatc acgtgggtga ccgcctgata ggggctgatg gtcggcaccg
     5281 gcaggctgtt gccggccgga gcgaccgtga ccgaacccgc gacgttgaga ccggccagcg
     5341 cctgatccgc gaccgtgccg gccggggcgt agatgaacgg aatcgccgtg ccggtgttgt
     5401 cgttgctctt ggccagcacc aggcctgggg ccggggtctg cgccgtcgcc ttggcagtgc
     5461 tcgcgttgag cgacgaggtc gacgcgaaag tggccgcgtg ggtgtgggtg ggcaactggc
     5521 cgctgttcag cgtcacgctc tccgcaccgc cgacctggcc gagctggacg ggcgacaggc
     5581 cgttgccctg gccccagtgc atcatcgagc gggaccgcag gtccggcagg ttgaacgtgg
     5641 tcgtcccgtt gccgccaaag gagacgccga gcagcgcgaa cagcgccgaa ttctgcgcga
     5701 tgcccagcga ctgcccgttg cacagcgcgg tgccgcgcgg agcatagttc cagccgccct
     5761 ggatgatctg tccaagatag aggtttgaca tggttgattt cctttgatcg gaaacacagc
     5821 cggcgggtgc tattcctgca ctttcgccga tgtatcgccg tgggggtgct ttagcttgtt
     5881 cttatcaacc cgatcgctgg aaatactata ggcaataaaa gagctgtcaa ttgcagcccg
     5941 tcgaaaatgt tcgttaatta ccgtaaagca aaattgcgct accttttctg tgccaattgc
     6001 tggcgcagcg ggtccagcca ctgggccgcc gcccggcgga tcgaatcggt tgcctccgcc
     6061 tgcttggccg cgcggtcggc cgcgaacggg cgctccgggg ccttggcatc gcgctgcgct
     6121 gccgccgcca tccgggcgcg ggccgcgctg tcgagctcca gcccgaaatg cgccgcgatg
     6181 gccccggtcg ccgcggcggg gatctccggg tagtcgaccg ccagtccccc gccgagcggc
     6241 cagtgatcga tcaccgcccg catcgccgcg tgcagcacca tggctgcatg gtcgtccggc
     6301 ccttcggcct gcgccggatc gatccccagc agcgcgggat cgagcgcgcc gggcagaatg
     6361 tgcatgctgg gctgggcggc gagcgagacc agcacttcgg ccggatcgcg gtgcaggtag
     6421 acccagttca ccgccgggaa ggccgcgcgc agctggggca ggaacagggt gtgccagact
     6481 tccagcttga tgaaatgccg gggggccggg tggcggggcc ggcccagggc ggccaccatg
     6541 gcccggatcg cggccgagga ctcggccggg tccggccgcg ccgccgcggt ccagcgcagc
     6601 acggcgtcga gcggctcggg ctcggacgac accgtgtggc cgggcagggc ccccagcatc
     6661 tgcgccagca gggtcgatcc gcagcgcgag aggtgaaaga ccaggccgga gagcggcggt
     6721 tccggttcgg ccggagcgcc cttgaccaga tcccgcagcc cggtgcggat ccgcagcagc
     6781 cagttgagcg gcagggcgct ggcccggcgg acctcgtctt cgaagaacgg cgcgtcgagc
     6841 cgccgcgaac cgaaccacag ccagtcgaac gccggcgcgt cgccggtcgg caccgaacgg
     6901 gtcggcagcc accccggcgg cggccagccg tccagcgcga ccggcgcggc ggcgaagcgc
     6961 cccagcccga gcggatcggg ccgcagcacc gcgcgcagcg cttcggcatc gcaggccacc
     7021 ccgtgctcgg ccgccacggc cagggcctcg gcggcaaagg catcggccgc caggatcgcg
     7081 ccaagctggt cctgcaggtc cggacgctcc agcaacagcc gcaccagggc gccgagcgaa
     7141 gcgggcaggc caagggccat agcgggcagc ggctgcggga gtgtcatcac cgccaatgat
     7201 agccgccccc gggggcttgt cgaccgggag agaagcgagg cgaaaaattc cc
//
LOCUS       DASUDV010000382         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2001056,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000382 DASUDV010000000
VERSION     DASUDV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001056"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="VFV30_08330"
     CDS             complement(<1..1913)
                     /locus_tag="VFV30_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX4848137.1"
                     /translation="MHWKVTAAAVLALAVHAPAWADTAAPATAAPAVPAGKLTGQVQP
                     QAYRLDLTIDPRQERFSGKTEIDAVLKEPSRTIDLHWRDLNLTAVEVLAGGQVQKPVA
                     QQLDPTGVGRLTFAENVPAGPITLRFAYDAAFQTAPAGLFRVKVADEWYAWTQLQSID
                     GRGVFPSFDEPGFKTPFTVTLRTPPGQTAVSNAPQTGVTREGGLDVHTFAPTLPLPTY
                     LVAAMVGPFAVVEGTVPPSPQRAKPLPLRIISPKSNADRLSFALEGSKEIVRLLEAYF
                     NDAFPYPKLDQITSPIMPGAMENAGADLYNDAIIVMDEKTTTSQKRTFGMVVAHELAH
                     QWFGDLVTPAWWDDIWLNESFANWMGFRIGDEWRPALNIRAGAVQEGFEAMRTDALVA
                     GRPIRQPIRTNAQIDEAFDSITYGKGGHVVAMIAGYMGDDKFRDGVRRYMAAHRYGNA
                     TSTDFFKAMAEASGDPRIVPAMQSFTDQQGVPLLTIARAGKSWQVSQSRYARLGATPP
                     ETRWGIPLCLRTTGAARQCQLLTEAKTTISYNGKGALMPNAGGTGYYRFELPAAEWDK
                     LIAAADRLPGGEAQALADSLDASFKAGRATPAQLITAARKLAQQADSYANGAGVAALA
                     GYAEAGFLEDAGKA"
BASE COUNT          329 a          693 c          714 g          346 t
ORIGIN      
        1 gccttgccag cgtcttcgag gaaccccgct tcggcatagc cggcaagcgc cgctactccc
       61 gcgccattgg catagctgtc ggcttgctgg gccagcttgc gcgcagcggt gatcagctgt
      121 gccggggtgg cgcggccagc cttgaagctg gcgtcgagcg aatccgccag ggcctgggct
      181 tcgccacctg gcaggcgatc cgccgccgcg atcaacttgt cccactccgc cgccggcagt
      241 tcgaaacggt aatagccggt cccgccggca ttgggcatca gtgcgccctt gccgttgtag
      301 ctgattgtcg tcttcgcctc ggtcagcagt tggcactgcc gcgccgcgcc ggtggtgcgc
      361 aggcacaaag gaatgcccca gcgcgtttcg ggcggcgtcg cgcccagccg ggcatagcgg
      421 ctctggctga cctgccagct cttgccggcc cgggcaatgg tcagcagcgg cacgccctgc
      481 tgatcggtga agctttgcat cgccggcacg atccggggat cgcccgaagc ctcggccatg
      541 gccttgaaga aatcggtgct ggtggcgttg ccatagcggt gggccgccat atagcgccgc
      601 accccatcgc ggaacttgtc gtcacccatg tagccggcga tcatcgccac cacgtggccg
      661 cccttgccgt aagtgatcga atcgaaggct tcgtcgatct gggcgttggt gcggatcggc
      721 tggcggatcg gccgcccggc gaccagcgca tcggtgcgca tcgcctcgaa cccttcctgc
      781 accgccccgg cgcggatgtt gagcgccggg cgccattcgt cgccaatccg gaagcccatc
      841 cagttggcga agctttcgtt cagccagata tcgtcccacc aggccggggt gacgagatcg
      901 ccgaaccact ggtgtgccag ctcgtgcgcc acgaccatgc cgaaggtgcg cttctggctg
      961 gtggtggtct tctcatccat cacgatgatc gcatcgttgt agaggtccgc cccggcattc
     1021 tccatcgccc ccggcatgat cggcgaggtg atctgatcga gcttgggata ggggaaggcg
     1081 tcgttgaaat aggcttcgag cagccggacg atttcctttg acccttccag cgcaaagctc
     1141 agccgatcgg cgttggactt gggcgaaatg atccgcagcg gcagcggctt ggcgcgctgc
     1201 gggctgggcg gcacggtgcc ttcaaccacg gcaaagggcc cgaccatcgc cgcgaccaga
     1261 taggtcggca gcggcagggt cggggcaaag gtatggacat ccagcccgcc ctcgcgggtg
     1321 acgccggtct gcggggcatt gctgaccgca gtctgccccg gcggggtgcg cagcgtgacg
     1381 gtgaagggcg tcttgaagcc gggttcgtca aagctcggga agacgccgcg cccgtcgatc
     1441 gattgcagct gggtccaggc ataccattcg tccgccacct tgacgcggaa caggccggcg
     1501 ggcgcggtct ggaacgcggc gtcataggca aagcgcagcg tgatcgggcc ggccgggacg
     1561 ttttcggcaa aggtcaggcg gccgacgccg gtcggatcaa gctgctgcgc gacaggcttc
     1621 tgcacctggc cgccggccag cacctcgacc gccgtcaggt tgagatcgcg ccagtgcagg
     1681 tcgatcgtcc ggctcggttc cttcagcaca gcgtcgatct cagtcttgcc cgagaaccgc
     1741 tcctgccggg gatcgatcgt caggtcgagc cgataggcct gcggctggac ctgaccggtc
     1801 agcttgccag ccgggaccgc cggcgcggct gtggcaggcg cagcggtatc agcccaggca
     1861 ggcgcgtgga cggcaagggc caggacggcc gcagcggtaa ctttccagtg catcaggatc
     1921 cccatccaaa tgaattcaag cgccgcgcct gcgcgactgg cgcgcgcggc gggcacgatg
     1981 ttcgaccggt ggactacgca gatcgacgga gggctagcgc agcaacccgc cgagcaggcc
     2041 gcgggcgaag cggccgagca tagccccgcc cagcgccgtg cc
//
LOCUS       DASUDV010000383         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_543271,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000383 DASUDV010000000
VERSION     DASUDV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543271"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VFV30_08335"
     CDS             complement(<1..1374)
                     /locus_tag="VFV30_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX4848138.1"
                     /translation="MLATAAQADEGMWTFDGFPEAKMKADYGWAPDAKWLDRTRAAAV
                     RLTGGCSASFVSPDGLILTNHHCIVTCAQDLSTADNDLVANGFVATTRELERKCPGQQ
                     AEVVTAISDVTGAIKGAMGTLAGVELVKARDAKIAEIEKAGCTDQATTRCQVVTLFGG
                     GQYKLYTYRKYSDVRLVWAPEFAAAFFGGDPDNFNYPRYAMDASFLRAYENGKPVKVK
                     SFLKWNPRAPQPGEATFVVGNPGSTQRLFTQDQLAFQREVGLPITLATMSELRGRLLE
                     AMQGNADRTREGADTLFGVENSLKVMIGRQKALNDPAFTKRLADAEADLKARSAGNTE
                     VGDPWSDVAKAIGEYRKIYLPYRFVGGNGDLYGWAITLVRAAEERTKPNGERLPGFTD
                     SALPLTEKGLLDAKPTYPWLEELMMRYSLSKAREYLGANDPDTKLLLGRDSPEGLAAQ
                     LVQGSKLS"
     gene            1558..>2204
                     /locus_tag="VFV30_08340"
     CDS             1558..>2204
                     /locus_tag="VFV30_08340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848139.1"
                     /translation="MWLLDKMLKALVRQGRLVVTDWDGKRYSYGDPAADPVSIRLTDK
                     GAALHIARDPRVGAGEAYMDGRLVVEAPHDIRDMVLLVMANANRPGGRIGAPNPAKRT
                     LDWILAKADQINLRPKASSNVTHHYDLTRQFYELFLDEDRQYTMAYFKDPANSLERAQ
                     LDKQALIAAKLRLEPGMRVLDIGCGWGGLGLYLNRHFGCEVLGVSLAPDQVRFAN"
BASE COUNT          383 a          718 c          703 g          400 t
ORIGIN      
        1 ggaaagcttc gagccctgga ccagctgcgc ggcgaggcct tcgggcgaat cgcggcccag
       61 cagcagcttg gtatcgggat cgttggcgcc caggtattcg cgcgccttgg acaggctgta
      121 gcgcatcatc agctcttcca gccagggata ggtcggcttg gcatcaagca ggcccttctc
      181 ggtcagcggc agggccgaat cggtaaagcc gggcaaccgt tcgccattgg gcttggtccg
      241 ttcctcggcg gcacgaacca gcgtgatcgc ccagccgtag agatcgccgt tgccgccaac
      301 gaagcggtaa ggcaggtaga tcttgcggta ttcgccgatc gccttggcca catcgctcca
      361 cggatcgccg acttcggtgt tgcccgcgct gcgcgccttg agatcggctt cggcatcggc
      421 cagacgcttg gtgaaggccg gatcgttcag cgccttctgc cgcccgatca tgaccttgag
      481 cgaattttcc accccgaaca gggtatcggc gccttcgcgg gtccggtcgg cattgccctg
      541 catcgcctcg agcaggcggc cgcgcagttc cgacatggtc gccagcgtga tcggcaggcc
      601 aacctcgcgc tggaaggcca gctgatcctg ggtgaacagg cgctgcgtcg atccggggtt
      661 gccgaccacg aaggtcgcct cgcccggctg cggcgcgcgc gggttccact tgaggaagct
      721 cttcaccttg accggcttgc cgttttcgta agcccgcagg aacgaggcat ccatcgcata
      781 gcgcggatag ttgaagttgt ccggatcgcc gccgaaaaat gccgcggcga attccggcgc
      841 ccagaccagc cgcacatcgg aatacttgcg gtaggtgtaa agcttgtact gcccgccgcc
      901 gaacagcgtc accacctggc agcgcgtggt ggcctgatcg gtgcacccgg ccttttcgat
      961 ctcggcgatc ttggcatcgc gcgccttgac cagttcaacc ccggcgaggg tgcccatcgc
     1021 ccccttgatc gccccggtca cgtcgctgat cgcggtgacc acttcggcct gctggccggg
     1081 gcacttgcgt tccagttcgc gggtggttgc aacaaagccg ttggcgacca gatcgttgtc
     1141 ggcggtcgaa agatcctgcg cgcaggtgac gatgcagtgg tggttggtga ggatcagccc
     1201 atcgggcgaa acgaagctgg ccgaacagcc cccggtcagc cgaaccgccg cggcgcgggt
     1261 gcgatcgagc catttggcat cgggcgccca gccataatcg gccttcatct tcgcttcggg
     1321 aaagccatcg aaagtccaca tgccttcgtc ggcctgggcc gcagtggcca gcataagcgg
     1381 ggccgcaaac gcggttccgg caagaacaat ttgacgcaat ttcatcgaga tcctcatgat
     1441 gcttgaatgc ccgcgccaag ctctggcgcg ccatggcgcc acaatggcac ggcaggctgt
     1501 gttggcaagg ggacagaacg aacttgcagc cacgcgcctg tgggatattc tttgcccatg
     1561 tggctgctcg acaagatgct gaaggctctc gtccgccagg gccgactggt cgtcaccgac
     1621 tgggatggga agcgctattc ctatggcgat ccggctgccg atccggtctc aatccgcctg
     1681 accgacaagg gcgccgcgct gcacattgcc cgcgatccgc gtgttggtgc gggcgaggcc
     1741 tatatggacg ggcggctggt ggtcgaagcg ccgcacgaca tccgcgacat ggtgctgctg
     1801 gtcatggcca atgccaaccg gccgggtggc aggatcggcg cgcctaaccc ggccaagcgc
     1861 acgctggact ggatcctcgc caaggccgac cagatcaatt tgaggcccaa ggcatcgagc
     1921 aacgtcaccc accattacga tctgacccgc cagttctacg agctgttcct cgacgaggac
     1981 cggcaataca ccatggccta tttcaaggac ccggccaatt cgctcgaacg cgcgcagctc
     2041 gacaagcagg ccctgatcgc cgccaagctc aggctcgaac ccggcatgcg ggtgctcgat
     2101 atcggctgcg gctggggcgg cctggggctt tatctcaacc gccatttcgg ctgcgaggtg
     2161 ctgggcgtca gcctcgcccc cgatcaggtc cgcttcgcca acga
//
LOCUS       DASUDV010000384         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_255644,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000384 DASUDV010000000
VERSION     DASUDV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255644"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFV30_08345"
     CDS             complement(<1..833)
                     /locus_tag="VFV30_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4848140.1"
                     /translation="MKRTTGQDRSITRNWRRATQAVRAGTWRSEQGETSEALFLTSGY
                     CYDNAETAAMRFRGEDPGMTYSRLQNPTVAMLEERIAMMDGAEACRVQASGMAAMTTA
                     LLCQLSAGDHVVAGRAAFGSCRWLVDQLFPRFGIEATTIDARDNAQWEAAIRPNTKVF
                     FFETPANPTMDIVDLKFVCDLAKSRGITTVVDNAFATPALQRPIEWGADVVAYSATKL
                     MDGQGRVLAGAVSGSAEFINNVLLPFQRNTGPNLSPFNAWVVLKGLETLDLRIRRQSE
                     NA"
     gene            914..2206
                     /locus_tag="VFV30_08350"
     CDS             914..2206
                     /locus_tag="VFV30_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX4848141.1"
                     /translation="MPGRPSASFTTDQDPADWCAVITVFFLALVWWRLAIPSEIYFDE
                     IHYVVAARKALEGIRANPEHPLLAKSILAQMIHWFGDRPIVWRVPSALFGALGLYAFS
                     RLVWFASGSARAAILAALLLATNFFWFIHSRIAMLDMTMAGLAMAALWQIAAAIRLPH
                     QGRWRLALGGVLMGLALGAKWSVAPLLVLPGLLFLAGKLRAQGRRFLFARDGGPVPGI
                     SLVEAGVWLGLLPLAVYWATYWPGFHWQAKPIEWWRPIAWHEYMLGLQDSVRKPHPYR
                     SVWYEWVSNWRAIWYLYKDVDGAQRGVVLIGNPFTMYAGLAALVWALWTGLRRRRADL
                     LAFCALYLTSLGFWVVSSKPIQFIYHYLLPATFLMALLALALDAAWKRDDRWRWLGPA
                     GLALSFGFFAWFYPIISGYPLCCGRPSFEFWMWLRSWR"
     gene            2252..>2797
                     /locus_tag="VFV30_08355"
     CDS             2252..>2797
                     /locus_tag="VFV30_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX4848142.1"
                     /translation="MTVTSQPLDFTRGPAMANRFMLAPLTNLQSNADGTLTDDEIHWL
                     TMRAKGGFGLVMTAAAHVQRQGQGFPGQLGIWSDDHLPRLKELADRIRAEGSLSAVQL
                     HHAGMRSPADLIGEAPQGPFADAETGTRALSTAEVEQLRDDFIQAAVRAEQAGFDGVE
                     IHGAHGYVLAQFLDAENNRRDD"
BASE COUNT          387 a          969 c          931 g          510 t
ORIGIN      
        1 gcgttctcgc tctgccggcg aatccgcagg tccagcgttt ccagcccctt cagcaccacc
       61 caggcgttga acggcgagag gttgggcccg gtgttgcgct ggaacggcag cagcacgttg
      121 ttgatgaatt cggcgctgcc cgaaaccgcc ccggccagga cccggccctg cccgtccatc
      181 agcttggttg ccgaataggc caccacatcg gcgccccatt cgatcggccg ctgcagcgcg
      241 ggcgtggcaa aggcgttgtc gaccacggtg gtgatcccgc ggctcttcgc cagatcgcag
      301 acgaatttca gatcgacgat gtccatcgtc gggttggccg gggtttcgaa gaagaacacc
      361 ttggtgttgg ggcggatcgc cgcttcccac tgggcgttgt cgcgcgcgtc gatcgtcgtt
      421 gcctcgatcc cgaagcgcgg gaacagctgg tcaaccagcc agcggcacga tccgaacgcc
      481 gcccgcccgg ccaccacgtg gtccccggcg gacagctggc acagcagcgc ggtggtcatc
      541 gccgccatgc ccgatgcctg gacccggcag gcctcggccc cgtccatcat cgcgatccgc
      601 tcttccagca tggccacggt cgggttctgc aggcgggaat aggtcatccc cggatcttcg
      661 ccgcggaacc gcatcgccgc ggtttcggcg ttgtcgtagc aatagcccga ggtgaggaac
      721 agcgcctcgc tggtctcacc ctgctcgctc cgccaggtcc ccgcgcgcac ggcctgagtg
      781 gcgcggcgcc agttgcgggt gatcgagcgg tcctgtccgg tggtgcgttt catggtggct
      841 cgcgctgtaa gtgcggcggt gcagcggcgc aaggccgttg cgctccccgg catggcccgt
      901 taactcccgc cccatgccgg gtcgcccctc tgccagcttc accacggatc aggaccccgc
      961 agactggtgc gcggtcatca ccgtgttctt cctggctctg gtctggtggc ggctggcgat
     1021 ccccagcgag atctatttcg acgagatcca ctacgtcgtt gccgcacgca aagcgctgga
     1081 gggtatccgc gccaacccgg aacatccgct gctggccaaa tcgatcctgg cccagatgat
     1141 ccactggttc ggggaccggc cgattgtctg gcgggtgcct tcggcgctgt tcggggcgct
     1201 gggcctttat gccttttccc ggctggtgtg gtttgccagc ggcagcgccc gcgcggcgat
     1261 cctggccgcc ctgctgctcg ccaccaactt cttctggttc atccacagcc ggatcgcgat
     1321 gctggacatg accatggcgg gcctggccat ggccgcgctg tggcagatcg cggcggcgat
     1381 ccgcctgccc caccagggcc gctggcggct ggcgctgggc ggggtgctga tgggcctggc
     1441 gctgggcgca aagtggtcgg tcgcgccgct gctggtcctg ccggggctgc tgttcctggc
     1501 gggcaagctg cgtgcccaag gccgccgctt cctgtttgcg cgggatggcg ggccggtgcc
     1561 ggggatctcg ctggtcgaag ccggggtctg gctggggctg ctgccgctgg cggtctattg
     1621 ggcgacctat tggccgggct ttcactggca ggccaagccg atcgaatggt ggcgcccgat
     1681 cgcctggcac gaatacatgc tgggcctgca ggacagcgtc cgcaaacccc acccctaccg
     1741 ctcggtctgg tacgaatggg tcagcaactg gcgggcgatc tggtatctct acaaggatgt
     1801 cgacggggcc cagcgcgggg tcgtgctgat cggcaacccc ttcaccatgt atgcgggcct
     1861 cgccgcgctg gtctgggcgc tgtggaccgg gctgcgccgg cgccgggccg atctgctggc
     1921 cttctgcgcg ctgtacctca ccagcctggg gttctgggtc gtctcctcga agccgatcca
     1981 gttcatctac cactacctgc tgccggccac cttcctgatg gcgctgctgg ccctggcgct
     2041 ggacgcggcc tggaagcggg acgaccgctg gcgctggctc ggcccggccg gccttgcgct
     2101 cagcttcggc ttcttcgcct ggttctatcc gatcatctcg ggctacccgc tgtgctgcgg
     2161 ccggcccagt ttcgaattct ggatgtggct gcgcagctgg cgttaaccgc gcgattaaaa
     2221 tactctcggg tcaacaggga gattcgccgc aatgaccgtc acttcgcagc cgctcgattt
     2281 cacccgcggc ccggccatgg ccaaccgctt catgctggcc ccgttgacca acctgcaaag
     2341 caacgccgat ggcacgctga ccgatgacga gatccattgg ctgaccatgc gcgccaaggg
     2401 cggcttcgga ctggtgatga ccgccgcggc ccacgttcag cgccaggggc agggctttcc
     2461 cggccagctt ggcatctggt ccgacgatca cctgccgcgc ctgaaggaac tggccgaccg
     2521 gatccgcgcc gagggcagcc tctcagcggt gcagctgcac cacgccggca tgcgcagccc
     2581 ggccgatctg atcggcgaag cgccgcaggg cccctttgcc gatgccgaaa ccggcacccg
     2641 cgccctcagc actgccgagg tcgagcagct gcgcgacgat ttcatccagg ccgcggtccg
     2701 ggccgaacag gccggctttg acggggtcga gattcacggc gcccatggct atgtcctcgc
     2761 ccagttcctc gatgccgaga acaaccggcg cgacgac
//
LOCUS       DASUDV010000385         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_653784,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000385 DASUDV010000000
VERSION     DASUDV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653784"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..514
                     /gene="hspQ"
                     /locus_tag="VFV30_08360"
     CDS             134..514
                     /gene="hspQ"
                     /locus_tag="VFV30_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX4848143.1"
                     /translation="MDRATFFSPQAGRDIEAPRRARARFGIGDVVRHRVFDFRGVIFD
                     IDPVFANSEEWYEAIPKEVRPHREQPFYHLLAENGDSSYIAYVSQQNLLGDADGGPVD
                     HPNVAELFEEFDGARYRLRRQMTH"
     gene            complement(539..1429)
                     /locus_tag="VFV30_08365"
     CDS             complement(539..1429)
                     /locus_tag="VFV30_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4848144.1"
                     /translation="MANQPFPAAENAATAASAKGGAQASFPAPGVPHFTGFNRLGLWT
                     LYMKEVRRFFKVQTQTIWAPAMTTLLYLMIFTVAMGQGGRQVLGVSFASFIAPGLIVM
                     GMVNNAFANTSFNFLAGKIQGTIVDYLMPPISNVELILALIGAAVTRAVLVGLAIAAA
                     MLLWPGVSLAIAHPWAVVWFGFMGSVMLAIIGLITSVWAEKFDHAAMVTNFLIVPLTM
                     LSGTFYVIDNLNPMFQAVSRVNPFFYVISGFRFGFLGQSDIGDTNLAVLNGAIYVGVL
                     NLVLCGALYALLKSGWKLKS"
     gene            1501..2148
                     /locus_tag="VFV30_08370"
     CDS             1501..2148
                     /locus_tag="VFV30_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX4848145.1"
                     /translation="MSWTDERIEKLTKMWEGGATASQIADELGGVSRNAVIGKAHRLG
                     LKARPSPVKANEKEAAAPAPKAPKAESAPRPAPAPRPAPAAPRAAAPVANPAPAAPAS
                     EGPRIVSVGPGGFLRQGPGDQQAPIPPAPPRRLVPAKPSPEVADKTSLLDLNDRICRW
                     PMGHPGEPDFHFCGEKVNPGFPYCVEHCGRAYQAQLPRGHRRPPPPLPFGGPRVR"
     gene            2151..3119
                     /locus_tag="VFV30_08375"
     CDS             2151..3119
                     /locus_tag="VFV30_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX4848146.1"
                     /translation="MLRRALAAAAGALLAAGASAPALAWSEPTHQTTGAIAWLDLKAQ
                     HPAALAELLELIRQHPNYDLLEPVLAGLSPDQRERALFEVLARWPDDIRGGPEDRPEW
                     HYQLRVVSGRTWLWPLENGAAQAGFTYNFVTLANRCAAPADRAKAIGWLIHIVGDVQQ
                     PLHAGHQMTEAFPMTDRAGELAFVKRPNGETVNLHYYWDNTMEGSGVALPAGFEAWAD
                     ALAAKWPRSRLTRELNQPGDPLGLFRGYLDESAELARLLAYQGTYLRAAPDAAAAPQV
                     TAQEQAVAIALAERRIATGGYRIADVLARAIALSRAPGTTCQGAVP"
     gene            3150..>3601
                     /locus_tag="VFV30_08380"
     CDS             3150..>3601
                     /locus_tag="VFV30_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848147.1"
                     /translation="MTAHDPELGRPASGILGWLGFGGRQAAAPGGAPREARPAGERRG
                     SLQQRQLEEIGAFLTLHKLEVDADMLAIAWRYLTGSDSQVVRAIDRRLQLSEPVTAEW
                     LAEELGPQQSGDDAALLAQLMERLENSIDEFGRTSRDAQTATREYNSAL"
BASE COUNT          611 a         1282 c         1219 g          489 t
ORIGIN      
        1 ggcccggtcg ggtcgaagag ccgcgcggtt agccgatggc ggggcgcttg ccaagccccc
       61 tcgccgccct ttttggggga cggcccgctg caagccgttg tttcgcaggc gcacaacccc
      121 tatgttggca gcaatggacc gggcaacctt cttctctccg caggcaggcc gcgacatcga
      181 agcaccgcgc cgggcgcgcg cgcgcttcgg gatcggcgat gtcgtgcgcc accgggtgtt
      241 cgatttccgc ggggtgatct tcgatatcga tcccgtcttc gccaacagcg aggaatggta
      301 cgaggcgatc cccaaggagg tccgcccgca ccgcgaacag ccgttctacc acctgctcgc
      361 cgaaaacggg gattcaagct acatcgccta tgtcagccag cagaacctgc tgggcgacgc
      421 cgatggcggc ccggtggacc accccaacgt ggccgaactg ttcgaggaat tcgacggcgc
      481 ccgttaccgc ctgcgccggc agatgaccca ctaggccggg cggcccggcg ccgcccgttc
      541 agctcttcag cttccacccg ctcttcagca gggcatagag cgcgccgcac agcaccaggt
      601 tgagcacgcc gacatagatc gcgccgttga gcaccgccag gttggtatcg ccgatgtcgc
      661 tttgccccag aaagccgaag cggaagcccg agatcacgta aaagaacggg ttcacccggc
      721 tgaccgcctg gaacatgggg ttgaggttgt cgatcacata gaaggtgccc gacagcatgg
      781 tcagcggcac gatcaggaaa ttggtgacca tcgccgcgtg atcgaacttt tcggcccaga
      841 ccgaggtgat caggccgatg atcgccagca tcaccgatcc catgaagccg aaccagacaa
      901 ccgcccaggg gtgcgcgatc gccaggctga cccccggcca cagcagcatc gccgcggcaa
      961 tcgccaggcc gaccagcacc gcccgggtca ccgccgcgcc gatcagcgcc aggatcagtt
     1021 ccacgttgga aatcggcggc atcaggtagt cgacgatcgt gccctggatc ttcccggcca
     1081 ggaaattgaa actggtgttg gcaaaggcgt tgttgaccat gcccatcacg atcaggcccg
     1141 gagcgatgaa gctggcgaag gacacgccca gcacttgccg cccaccctgg cccatggcca
     1201 cggtgaagat catcagataa agcagcgtgg tcatcgccgg ggcccagatc gtctgggtct
     1261 ggaccttgaa gaaacggcgg acctccttca tatagagggt ccacagcccc agccggttga
     1321 agccggtgaa gtggggcacc cccggagccg gaaagctggc ctgcgcaccg cccttggcgg
     1381 atgcggcggt tgcggcgttc tcggcggcgg gaaaaggttg attggccatg ctgtggcgac
     1441 tatccgcagc ctgcgcccat ggcaagcatg cgccgcggcg ccgctgagga aagaagaccg
     1501 atgagctgga ccgacgaacg gatcgaaaag ctgaccaaga tgtgggaagg cggggccacc
     1561 gccagccaga tcgccgacga actcggcggg gtcagccgca atgccgtgat cggcaaggcc
     1621 caccgcctgg ggctgaaggc ccggcccagc ccggtcaagg ccaacgaaaa ggaagccgcc
     1681 gcccccgcgc ccaaggcgcc caaggcggaa agcgcgccgc gcccggcccc tgcgccgcgt
     1741 ccggccccgg ccgccccgcg cgcggccgct ccggtggcca atccggcccc ggccgctcct
     1801 gccagcgaag gcccgcggat cgtctcggtc ggccccggcg ggttcctgcg ccagggtccc
     1861 ggtgatcagc aggccccgat cccgccggcc ccgccgcgcc gcctggttcc ggccaagccc
     1921 agccccgaag tggccgacaa gaccagcctg ctggacctga acgaccggat ctgccgctgg
     1981 ccgatgggcc accccggtga gccggacttc cacttctgcg gggaaaaggt gaaccccggt
     2041 ttcccctatt gcgtcgagca ttgcggccgc gcctatcagg cccagctgcc gcgcggccat
     2101 cgccgcccgc ccccgccgct gccgttcggc ggcccgcgag tccgctaagc gtgctgcgcc
     2161 gggctctggc cgccgccgcg ggcgcgctgc tcgctgcggg ggccagcgcc ccggccctcg
     2221 cctggtccga acccacgcac cagaccaccg gcgcgatcgc ctggctggac ctgaaggcgc
     2281 agcatccggc cgcgctggcc gaactgctgg agctgatccg ccagcatccg aactatgacc
     2341 tgctggagcc ggtcctggcc gggctgagcc ccgaccagcg cgaacgcgcc ctgttcgaag
     2401 tgctggcccg ctggcccgac gatatccgcg gcggaccgga agaccggccc gaatggcatt
     2461 accagctgcg cgtggtgagc gggcggacct ggctgtggcc gctggaaaac ggcgcggcgc
     2521 aggccggctt tacctacaac ttcgtcaccc tggccaaccg ctgcgcggcc ccggccgacc
     2581 gggccaaggc gatcggctgg ctgatccaca tcgtcggcga tgtgcagcag ccgctccacg
     2641 ccgggcacca gatgaccgaa gcgttcccga tgaccgaccg ggcgggcgaa ctggccttcg
     2701 tcaagcggcc gaacggcgag acggtgaacc tgcattacta ttgggacaac accatggagg
     2761 gtagcggcgt ggccctgccc gccgggttcg aagcctgggc cgatgccctg gccgccaagt
     2821 ggccgcgcag ccggctgacg cgcgaactga accagcctgg tgatccgctg gggctgttcc
     2881 gcggctatct ggacgaaagc gccgaactgg cgcggctgct ggcctaccag ggcacctatc
     2941 tgcgggccgc gcccgatgcg gccgcagcgc cccaggtgac ggcgcaggag caggccgtgg
     3001 cgattgccct ggccgagcgg cggatcgcca ccggcggcta ccggattgcc gacgtgctgg
     3061 cccgggccat cgccctgtca cgcgcgcccg gcacgacctg ccagggggcc gtcccttaac
     3121 tttccggcgc ggcggccgtt aaccatggca tgaccgcgca cgatcccgaa cttggccgcc
     3181 cggcaagcgg catccttggc tggctgggct ttggcgggcg ccaggccgcc gcgcccggcg
     3241 gcgcgccgcg cgaggcccgg cccgcgggcg aacggcgcgg atcgctgcag cagcgccagc
     3301 tggaggagat cggcgcgttt ctgaccctgc acaagctgga ggtcgacgcc gacatgctag
     3361 ccatcgcctg gcgctatctg accggttcgg acagccaggt ggtccgcgcg atcgaccggc
     3421 ggctgcagct gagcgaaccg gtgaccgccg aatggctggc cgaggaactg gggccgcagc
     3481 agagcggaga cgatgcggcg ctgctggccc agctgatgga gcggctggaa aactcgatcg
     3541 acgaattcgg ccggaccagc cgcgatgcgc agaccgcgac ccgcgaatac aacagcgcgc
     3601 t
//
LOCUS       DASUDV010000386         7231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1205893,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000386 DASUDV010000000
VERSION     DASUDV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205893"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFV30_08385"
     CDS             <1..352
                     /locus_tag="VFV30_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX4848148.1"
                     /translation="MRAAAASFDFLLDTIPVKHDLTRYLFLLDRKGTLCIVGMIEPIE
                     PFHSGLLLGGQKAVSGSGVGGIAQTQEMLDLCAEKGILAEIEVIPVNQINHAFERMER
                     ADVKYRFVIDMRTL"
     gene            349..999
                     /locus_tag="VFV30_08390"
     CDS             349..999
                     /locus_tag="VFV30_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX4848149.1"
                     /translation="MTHTVDLFWSFRSPYSYIVVPKLQELERDWDAVVNVRPVLPIAV
                     RQPDFFAQADPLWFSYLMRDCVRSAEFSGMPLRWPRPDPVVMDFATRTYPKEQPYIHR
                     LTRLGVLASERGFGLSLLRAVSHLIWSGETEGWHEGDHLARAVAEAGGDLAEMDAVQE
                     AEAERLDAVIKQNEADQRLGGHYGVPLMVYDGEPFFGQDRYDQLVWRMQQKGMTAR"
     gene            complement(996..1952)
                     /gene="tilS"
                     /locus_tag="VFV30_08395"
     CDS             complement(996..1952)
                     /gene="tilS"
                     /locus_tag="VFV30_08395"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX4848150.1"
                     /translation="MPGDLPPPDPALTARFAADLAALWPGDGRLALAVSGGPDSLALL
                     RLAQAVLPDRIAAATVDHGLRAESAAEAAHVAAVCARLGVPHAVLKVALAEGNIQAEA
                     RAARYAALAEWMERDGLAALATAHHADDQAETLVMRLGRGSGVAGLAGVRARAAVPGT
                     RLPLLRPLLGWRRAELAAVAAPLDPVQDPSNSDARFDRARIRKALADADWLDVPALAQ
                     SAANLADADAALDWAAAREWAEGVSKGPMGYIYRPQAPRAVALRVLARLITELDGAEP
                     RGSAVARLFDSLLARQPASIGGLVARVMPDGWSFTKAPVRRS"
     gene            complement(1962..2924)
                     /locus_tag="VFV30_08400"
     CDS             complement(1962..2924)
                     /locus_tag="VFV30_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4848151.1"
                     /translation="MKQLFANKPLAVVVGLALAAPALAQTSDANTEARLRKLEAEVSA
                     IQRVVFPGGDGKFFPQLQPGQPGAAPGTPASAPAADLLGRMDALEGQVARLTAQVEED
                     RNRLEKLEARLAALEAGRTTPAAPAASGSPTDTNLAAMTSGASTTTKPAPAATTPPPA
                     PKPAPVAKPADPSAKRLAAVRAIAKPQTDDPGDDEYSYGFRLWEAKFHPEAQQQLKLF
                     LNKYPKHRRVSWARNLLGRSYLDEGNPREAASWFLQNYRADPKGDRAPDSLLFLAESM
                     RQLKDTGRACVAIEQFGKEYPKEAAGRLKAQYDLTRGGLKCPAA"
     gene            complement(3084..4052)
                     /locus_tag="VFV30_08405"
     CDS             complement(3084..4052)
                     /locus_tag="VFV30_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4848152.1"
                     /translation="MTDVEMTEAELPLESAGARMLRAREESGMSRSQMAALTRIPERH
                     LAAIEAGDFAALPARTYAVGFSRSYAKALGLDPEPVVAAVREELAAQQPADTRRTVQT
                     FEPGDPARVPGARLAWFAGLGILAVVVIGYFAVRSMFAPGMTLPSILKDDPPAAAASQ
                     AAPPPAAPTPAGPVVFTATQPKVWVKFTDAAGNQLFQKELAQGESWTVPADAADVRIR
                     TARPDALAITIGGQPVPPLSDVQTTISDVPVTAAALLARGTPAAVPAPAPTANAAPAR
                     SAAPQRQRERRAPAPAAGETGAPAAAATPEPVPAATAAPAPAATAT"
     gene            complement(4197..6473)
                     /gene="ptsP"
                     /locus_tag="VFV30_08410"
     CDS             complement(4197..6473)
                     /gene="ptsP"
                     /locus_tag="VFV30_08410"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX4848153.1"
                     /translation="MPVTSAVSAARTILTRLHDVMASRSHAQAKLNTVVEVIGEGLDS
                     EVCSIYLLREGMLELFATKGLAQEAVHVTRMAIGEGLVGTIADNIETLNLAEATAHPD
                     FSYRPETGEEKFHSFAGVPIVRRERAVGVLCVQHADPRRYDEVEIEALQTVAMVLSEL
                     ISNAGLVDDEDVALGPQQTGPVRMTGLTLVKGLAAGNAVFHQPRITIEHVMAEDRDAE
                     LQRVYLAFDKMREQIDRMASQAEFGVGGEHEEVLETYKMFAYDEGWSRRIIEAIDSGL
                     TAEAAIERVQQRTRMRMRQIDDPLLADRMHDLEDLSNRLLRIVSGQMGTAASQGLRSD
                     TILIARNLGPAELLEYDKRRLKGVILEEGSLTAHVVIVARAMGIPVLGRVRSLRGAVR
                     EGDPLLLDSDKGEATVRPIDAMVEAFEARFAKSKERAAAYAALRDVEPFTRDGTRITV
                     LMNAGLRDDMPNLALAGADGVGLFRTEFQFLVSATLPARERQTRLYRDVLDAAGDKPV
                     VFRTVDIGGDKVLPYLRHNNGEHEENPAMGWRALRVALERDGLLKVQARSLLEAAAGR
                     TLNVMFPMVSEPWEFDAAKAVFESQRKYLKGQKKLLPEAIRYGAMLEVPALAETLDIL
                     APRLSFLSIGTNDLTQFLFAADRSNPKLAERYDWLSIAIMRFLKRVQQNVVGHNVDVT
                     VCGEMGGRRLEALALLGIGIRRLSITPAGVGPIKELVRKVDLAEITEAMNGWLSAPPP
                     NLREALAAWAQERGIEAD"
     gene            complement(6602..6757)
                     /locus_tag="VFV30_08415"
     CDS             complement(6602..6757)
                     /locus_tag="VFV30_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX4848154.1"
                     /translation="MEQSHVSALQLKHAGLERQIQEELSRPMPDLAMVQALKKRKLRI
                     KEELAFH"
     gene            6944..7141
                     /locus_tag="VFV30_08420"
     CDS             6944..7141
                     /locus_tag="VFV30_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX4848155.1"
                     /translation="MNEEELKKRLEVLRIEHRDLDAAIDALTGAGATDQLQIARLKKR
                     KLKLKDQIAMIEDYLIPDIIA"
BASE COUNT         1072 a         2475 c         2520 g         1164 t
ORIGIN      
        1 gatgcgcgcg gctgcggcca gcttcgactt cctgctcgac acgatccccg tgaagcacga
       61 cctgacccgc tacctcttcc tgctcgatcg caagggcacg ctgtgcatcg tcggcatgat
      121 cgagccgatc gaaccgttcc attccggcct cctgctgggc gggcagaagg cggtatccgg
      181 atcgggcgtc ggcgggattg cccagaccca ggaaatgctg gacctctgcg ccgagaaagg
      241 cattctggcc gagatcgagg tgatcccggt aaaccagatc aaccacgcct ttgaacgcat
      301 ggagcgggcc gacgtgaagt accgcttcgt catcgacatg aggacgctat gacccatacc
      361 gtcgacctgt tctggtcgtt ccgcagcccc tattcctaca tcgtcgtgcc caagctgcag
      421 gaactggagc gcgattggga cgcggtggtg aacgtgcggc cggtcctgcc gatcgcggtg
      481 cgccagcccg atttcttcgc ccaggccgat ccgctgtggt tcagctatct gatgcgcgac
      541 tgcgtgcgca gcgctgaatt ttccggaatg ccgctccgct ggccgcggcc cgatccggtg
      601 gtgatggatt tcgccacccg gacctatccc aaggagcagc cctacatcca ccggctgacg
      661 cgccttgggg tgctggccag cgagcgcggc tttggcctgt cgctgctgcg cgcggtcagc
      721 cacctgatct ggtcgggcga gaccgaaggc tggcacgagg gcgatcacct ggcccgcgcc
      781 gtggccgagg ccggcggcga ccttgccgaa atggacgcgg tgcaggaggc cgaggccgag
      841 cggctcgacg cggtgatcaa gcagaacgag gccgaccagc gtctgggcgg gcactacggc
      901 gttccgctga tggtctatga cggggaaccg ttcttcgggc aggaccgcta tgaccagttg
      961 gtctggcgga tgcagcaaaa gggcatgacg gcgcgctagc tccgccgcac cggggctttc
     1021 gtaaagctcc acccatccgg catgacgcgg gcgacaaggc cgccgatcga ggccggttgc
     1081 cgggcgagca ggctgtcgaa caggcgggcc accgcgctgc cgcgcggctc cgcgccgtcc
     1141 agttcagtga tcagccgagc cagcacccgc agcgccaccg cgcgcggggc ctgcggccgg
     1201 tagatgtagc ccatcggccc cttgctgacg ccttcggccc attcgcgcgc ggcggcccaa
     1261 tcgagcgccg catcggcatc ggccaggttg gcggcgcttt gcgccagggc cggcacatcg
     1321 agccaatcgg catcggccag ggccttgcga atccgcgcgc ggtcgaaccg cgcgtcgctg
     1381 ttggagggat cctgaaccgg atcgagcgga gccgcgacag cggccagttc ggcccgccgc
     1441 cagcccagca gcggtcgcag cagcggcagg cgcgtgcccg gcaccgcggc gcgggcgcgg
     1501 accccggcga ggccggccac cccgctgccc cgccccagcc gcatcaccag cgtttcggcc
     1561 tggtcgtcgg cgtggtgcgc cgtggccagc gccgccagcc cgtcacgctc catccattcc
     1621 gccagcgccg catagcgcgc cgcgcgggct tcggcctgga tgttgccttc ggcgagagcc
     1681 accttgagca ccgcgtgcgg cacccccagc cgggcgcaga cggcagccac atgggccgct
     1741 tcggccgcgc tttcggcgcg cagcccgtga tcgaccgtgg ccgcggcaat ccggtccggc
     1801 aggacggcct gggccaggcg cagcagggcc agactgtccg gcccgcccga tacggccagg
     1861 gccaggcgtc cgtccccggg ccacagggcg gccaaatcgg cggcaaagcg cgccgtcagc
     1921 gcgggatcag gcggaggcag gtctccgggc aaggcgtgcc gtcaggccgc cgggcacttc
     1981 agcccgcccc gggtcagatc gtactgcgcc ttcagccggc cggcggcttc cttggggtat
     2041 tccttgccga actgctcgat cgcgacacag gcgcggccgg tgtccttcag ctggcgcatc
     2101 gattcggcca ggaacagcag gctgtccggc gcgcgatcgc ccttgggatc ggcgcggtag
     2161 ttctgcagga accagctcgc cgcctcgcgc gggttgccct cgtcgagata ggagcggccg
     2221 agcaggttgc gggcccagct gacccggcgg tgcttaggat acttgtttag gaacagcttg
     2281 agctgctgct gcgcctcggg gtggaacttc gcctcccaca ggcggaagcc ataggagtat
     2341 tcgtcgtcgc ccggatcgtc ggtctgcggc ttggcgatgg cgcgaaccgc cgccagccgc
     2401 ttggccgagg gatcggccgg cttggccacg ggcgccggct tgggcgcggg cggcggagtg
     2461 gtggcggccg gggccggctt ggtcgtcgtc gaggcaccgc tggtcatggc tgccaggttg
     2521 gtgtcggtcg gcgagccgct ggccgccggg gcagccggag tggtgcggcc ggcttccagc
     2581 gcggccagcc tggcttccag cttttcgagc cggttgcggt cttcctcaac ctgcgcggtc
     2641 agccgggcga cctggccttc cagtgcgtcc atgcggccga gcagatcggc cgccggtgcg
     2701 ctggccgggg tgccgggggc cgcgccgggc tggcccggct gcagctgcgg aaagaacttg
     2761 ccgtccccgc cggggaacac cacccgctgg atcgccgaca cttcggcttc cagtttgcgc
     2821 aggcgggctt cggtgttggc gtcgctggtc tgggcaagcg ccggagccgc aagggccagg
     2881 cccacaacca ccgccagagg cttgttcgcg aaaagctgct tcatcgtgtt tccatgctct
     2941 tgccgaccaa gggttgcacc gccatggccc cgacgggccg aaccggagat gaaccgcagg
     3001 gttcggggtt taagcgcagc gccgccagcc tgtcgagcgg gccggcgcgg ctatcctcaa
     3061 ttcgtttcag gcctcagttc gtttcaggtg gccgttgccg ccggagccgg agccgccgtg
     3121 gcggccggga ccggctcggg cgtggccgcg gcggcgggcg ccccggtctc gccggcggca
     3181 ggcgccgggg cgcgccgttc ccgctggcgc tgcggcgcgg cgctgcgggc cggagccgcg
     3241 ttggccgttg gcgccggggc cggcacggcg gcgggcgtgc cgcgcgccag cagggcagcc
     3301 gcggtgaccg gcacgtcgct gatcgtggtc tgcacatccg acagcggcgg aaccggctgg
     3361 ccgccgatcg tgatcgccag ggcatcgggc cgggcggtgc gaatccgcac atcggcggcg
     3421 tcggccggaa cggtccagct ttcgccctgg gccagttcct tctggaacag ctggttgccg
     3481 gcggcatcgg tgaacttgac ccagaccttg ggctgggtgg cggtgaagac caccggaccg
     3541 gccggggtcg gcgcagccgg cggcggagcg gcctggctgg cggcggcggc cggcggatcg
     3601 tccttgagga tcgagggcag ggtcatgccg ggagcgaaca tcgaacgcac ggcaaaatag
     3661 ccgatcacca ccacggccag gatgccgagg ccggcaaacc aggccagccg cgcgccgggc
     3721 acgcgggccg gatcgccggg ctcgaacgtt tgcaccgtgc ggcgggtgtc ggctggctgc
     3781 tgcgcggcca gttcctcgcg caccgcggcg accaccggtt cgggatcgag ccccagcgcc
     3841 ttggcatagg accgcgagaa gccgaccgca taggtccgcg ccggcagcgc ggcgaaatcg
     3901 cccgcttcga tcgcggccag atggcgttcg ggaatgcggg tcagcgcggc catttgcgaa
     3961 cgggacatgc cggattcttc acgggcacgc agcatccgcg caccggcact ttccaggggc
     4021 agttccgcct ctgtcatctc cacgtccgtc atgtcgaccc caatcaattg cagtccggtt
     4081 ttccggttgc gcgggacaat cccggtttgc ggttggaagt caacgcaaaa gcgcgccttc
     4141 ccgccgtttg cgtcggcagg atggggcgga tttgtgacag gccggagaaa ccgggctcag
     4201 tcggcctcga ttccgcgttc ctgcgcccag gcggccagcg cctcgcgcag gttgggcggc
     4261 ggggcagaga gccagccgtt catcgcctcg gtgatttcgg cgaggtcgac cttgcggacc
     4321 agttccttga tcgggccaac cccggccggc gtgatcgaga gccggcggat gccgatgccg
     4381 agcagggcca gcgcctcgag ccgccgcccg cccatttcgc cgcagaccgt cacgtcgacg
     4441 ttgtggccga ccacgttctg ctggacccgc ttcaggaacc gcatgatcgc gatcgatagc
     4501 cagtcataac gttcggccag cttggggttg gaccggtccg ccgcgaacag gaactgggtc
     4561 agatcgttgg ttccgatcga caggaacgaa aggcgcggcg ccaggatgtc gagcgtttcg
     4621 gccagggccg gcacttcgag catggcgcca tagcggatcg cctcgggcag cagcttcttc
     4681 tggcccttga ggtatttgcg ctggctttcg aacaccgcct tggcggcatc gaattcccac
     4741 ggttcgctga ccatcgggaa catcacgttg agcgtgcggc ccgccgccgc ctcgagcagc
     4801 gagcgcgcct gaaccttgag cagcccgtcc cgttccagcg ccacgcgcag cgcgcgccag
     4861 cccatcgccg ggttctcttc atgctcgccg ttgttgtggc gcaggtaggg cagcaccttg
     4921 tcgccgccga tatcgaccgt gcggaacacc acgggcttgt cgcccgcggc gtccagcacg
     4981 tcgcggtaca gccgggtctg gcgttcccgc gccggcagcg tggccgagac caggaactgg
     5041 aattcggtgc ggaacagccc caccccgtca gccccggcca gggccaggtt gggcatgtcg
     5101 tcgcgcaggc cggcgttcat cagcacggtg atgcgggtgc catcgcgggt gaagggttcg
     5161 acatcgcgca gcgcggcata ggcggcggcg cgctccttgc tcttggcaaa gcgggcctcg
     5221 aacgcctcga ccatcgcgtc gatcgggcgg acggtcgcct cgcccttgtc ggaatcgagc
     5281 agcagcggat cgccttcgcg caccgccccg cgcaaggacc ggacccggcc cagcaccgga
     5341 atgcccatcg cccgcgcgac gatgaccacg tgggcggtca gcgagccttc ctccaggatc
     5401 acgcccttca ggcggcgctt gtcatactcc agcagctcgg ccgggccgag gttgcgggcg
     5461 atcaggatcg tgtcactgcg gaggccctgc gacgcggccg tgcccatctg gcccgaaacg
     5521 atccgcagca gccggttaga gaggtcctcc agatcgtgca tccggtcggc cagcagcgga
     5581 tcgtcgatct gccgcatccg catccgggtg cgctgctgga cccgctcgat cgcggcctcg
     5641 gcagtaaggc cgctgtcgat cgcctcgatg atccggcggc tccagccttc gtcgtaagcg
     5701 aacatcttgt aggtctcgag cacctcctcg tgctcacccc ccacgccgaa ttcggcctgg
     5761 ctggccatcc ggtcgatctg ttcgcgcatc ttgtcaaagg ccaggtagac gcgctgcagt
     5821 tcggcgtcgc ggtcttcggc catcacgtgt tcgatcgtga tccgcggttg gtggaagacc
     5881 gcgttgccag cggccaggcc cttgaccagg gtcagcccgg tcatccgcac cggtccggtc
     5941 tgctgcgggc ccagcgcgac gtcctcgtca tcgaccaggc cggcgttgga aatcagctcg
     6001 gacaggacca tggcgacggt ctgcagcgcc tcgatctcca cctcgtcata gcggcgcgga
     6061 tcggcgtgct ggacgcacag gacgcccacc gcccgctcgc gccggacaat cggcaccccg
     6121 gcgaacgagt ggaacttttc ctcgcccgtt tcggggcggt aggaaaagtc cgggtgggcc
     6181 gtcgcttcgg ccaggttgag cgtttcgatg ttgtcggcga tcgtgccgac caggccttcg
     6241 ccgatcgcca tccgggtgac gtggacggct tcctgcgcca gccctttggt ggcgaacagc
     6301 tcgagcatgc cttcgcgcag caggtagatc gagcagactt cgctatcgag gccctcgccg
     6361 ataacctcga ccaccgtgtt cagcttggcc tgggcgtggc tgcgcgaggc catcacgtcg
     6421 tgcagacgcg tcaggatcgt gcgggcggcg gaaacggcgc tggttacggg catgggcgaa
     6481 gcctagccga ttgcgccccc cttgggaacg cgcgagcacg ccggtgcaaa cgaatttgtg
     6541 gacggaccgg accgcgtgcc ggttgcgcct ggcgccccgg ccgcagccct gtccgcgccg
     6601 gtcagtggaa ggccagctct tccttgatcc gcagctttcg cttcttcagc gcctgaacca
     6661 tcgcaaggtc aggcatcggg cggctcaatt cttcctggat ttgccgttcg agaccggcgt
     6721 gcttgagctg cagagcgctg acatgcgatt gttccatcgg ggattctcct tgtgttagcc
     6781 cccagggctg caaggacatc gccgtcaacc gactcagcat ccggcgagtc actgcgattg
     6841 tcatcaaaac acattaagcc tatcttgcga agcggatatg cgatccccct gcggcagggc
     6901 gccagcggcc tgcggcaaac cggcaagcgg agattttacg ggcgtgaacg aggaagagct
     6961 gaagaagcgc ctggaagtgc tgcggatcga gcaccgcgat cttgacgcgg cgatcgacgc
     7021 gctgaccggg gccggcgcca ccgaccagct ccagatcgcc cggctgaaga agcgcaagct
     7081 gaaactgaaa gaccagatcg cgatgatcga ggactacctg atccccgata tcattgcgta
     7141 atcgcagggt gcgctgatcg catccgcgat ccgcttggcc tggccggccc gctcctccgg
     7201 cccagccacc cgttaaaggt accctgtggg t
//
LOCUS       DASUDV010000387         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_874549,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000387 DASUDV010000000
VERSION     DASUDV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874549"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..2288
                     /locus_tag="VFV30_08425"
     CDS             123..2288
                     /locus_tag="VFV30_08425"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX4848156.1"
                     /translation="MTRVRLLVFNPALPVLDYRLPAGMATEFGQLVIAPLGPRQVLGI
                     VWEPERLPGDEVPEAKLRPILEVLPVPPLSAPLRRLIEWCADYYVAPMNAVARMALGS
                     MAALRGGGTTTEYRLSGEEPKRLTPQRAAAMDALHGEQASIRELAHIAGVSEGVLRGM
                     VGTGILEPITVDLDRPYPRARPDHAVPDLSPDQQAAADAFVGAVEAGQFVPFLLDGVT
                     GSGKTECYFEAVAEALRRGRQVLVLLPEIALTENFLRRFEARFGVPPVLWHSSLKQSE
                     RRRAWRAIGSGEAQVVVGARSALFMPYARLGLIVVDEAHEVSFKQDDGVRYNARDVAV
                     IRAKFESIPVILASATPALESLQLAEAGVYTKIDLPDRFGGASLPDVSIVDLRQEMPE
                     RGHWLAPRLVAEMRARLDRGEQTLLFLNRRGYAPLTLCRHCGYRFQCPNCSAWLVEHR
                     LSRRLACHHCGHEVPPPAACPECGEPDCLVACGPGVERIADEVAEVLPEARVAVVTSD
                     TLNSPEKISEFVAAAEEKRIDVIVGTQIVTKGYHFPDLTLVGVVDADLGLEGGDLRAA
                     ERTYQQVAQVAGRAGRAEKPGAVLIQTRHPEAAVIAALAAGDRDAFYEAEAEARKDAG
                     APPFGRWAAIIVSSEDEAEAREAARAVGGTAPNLPDVLVLGPAPAPLSLLRGRYRYRL
                     LINARRTAKLQDVIREWLAPLQFPQGVRISVDIDPYSFV"
     gene            2355..3863
                     /locus_tag="VFV30_08430"
     CDS             2355..3863
                     /locus_tag="VFV30_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848157.1"
                     /translation="MSVLLAVLAQCLVVPAAHAKWLRAETRNFVIYSSGSERELRRFA
                     SNLEMFDGLAQRRFGLEPNDNTNKLTIYFLDSQSDVSRLAGDKDRMVAGFYSARLEGS
                     FAVANRSRGDGMWDLNGMTVLFHEYAHHFMFRNFDFAYPAWYVEGFAEYLSTATFSAD
                     GSWTMGKPAFHRAYSLLEGKKLPIEKLLFSSVSDLKSDEVSVFYGRAWLLVHMLNMKP
                     EHQGKLKTYFTAIREGKPPREAAALAFGDLQQLDKSLDLYLRQRLSYYTSPTPIPADT
                     TTTVTELDEATGQLVGFRMLRLVGRMEPKAMAALRGLAAANLGSAEIWHELALAERQF
                     SGPGDDNEAARQAREKAAEAAVDRALAADPNHVRANVLKADILMTRLEEGRVTEPARW
                     SDARKFLITANKYAVDDPLVLFNWYNSFRLQGREPSATARAALARAFVLAPEVINVRV
                     AYAFDLADQRRFDEALGLVEFLAQHPHHAAEGRELVERIKEMRDRAAADKDS"
     gene            complement(3860..>4488)
                     /locus_tag="VFV30_08435"
     CDS             complement(3860..>4488)
                     /locus_tag="VFV30_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848158.1"
                     /translation="AEPFEAPLPAGYEPSGQPRVPGLYAEVLGLSSIFDERQLEELDE
                     ACAPRGKVVTLSGQENRGKGAVRIYRTATAVAVVTVTSNLSIDTETCTASYALTSRAT
                     VRTGKWKYSEVPYFQNPKLDCRPNRRIGRCSEQEHAGITVTCRNNGDGFVGDAICVSN
                     RKDLTRGLVFAYESYVDDDSMPTGGWALQRVETDALIDPAVFAKRDGG"
BASE COUNT          712 a         1543 c         1498 g          735 t
ORIGIN      
        1 agaaagctgc gccgcgccag cgaggcggtg atcatctttt ccataggtcc gctgctcctt
       61 cgcgttccgc atcgtcttgc gggggctgtc acccgccgcc gaatgcgcca tagaggccgt
      121 gcatgacccg cgtccgactc cttgtcttca acccggccct gccggtgcta gactatcgcc
      181 ttcctgctgg aatggcgaca gaattcgggc agctggtgat cgccccgctg ggcccccgcc
      241 aggtgctggg gatcgtctgg gagccggagc gcctgccggg cgacgaagtg ccagaggcaa
      301 agcttcgccc gatcctggaa gtgctgcccg ttccgccgct cagcgcgccg ctgcgccggc
      361 tgatcgaatg gtgcgcggac tattacgtgg cgccgatgaa cgcggtggcg cggatggcgc
      421 tgggctccat ggcggcgctg cgcggcggcg ggaccaccac cgaataccgc ctgtccggcg
      481 aagagcccaa gcgcctgacc ccgcagcgcg ccgccgcgat ggacgcgctc cacggcgagc
      541 aggccagcat ccgcgaactg gcgcacatcg ccggcgtttc ggagggcgtg ctgcgcggca
      601 tggtggggac cggcatcctc gaacccatca cggtcgatct tgaccgcccc tacccccgcg
      661 cccggcccga ccacgccgtg ccggacctct cccccgacca gcaggccgcc gccgatgcct
      721 tcgtcggcgc ggtggaggcc gggcagttcg tccccttcct gctcgacggg gtaaccggat
      781 cgggcaagac cgagtgctat ttcgaagccg tcgccgaagc gctgcggcgg ggccgccagg
      841 tgctggtcct gctgccggaa attgccctca ccgagaactt cctgcgccgt ttcgaggcgc
      901 gctttggcgt gcccccggtg ctgtggcatt ccagcctgaa gcagagcgag cggcgccggg
      961 cctggcgcgc gatcggcagc ggcgaggcgc aagtggtggt cggcgcgcgc tcggcgctgt
     1021 tcatgcccta tgcccggctg ggcctgatcg tggtggacga ggcccacgag gtctcgttca
     1081 agcaggacga cggggtgcgc tacaacgccc gcgacgtggc cgtgatccgc gccaagttcg
     1141 agagcattcc cgtgatcctg gccagcgcca ccccggcgct tgaatcgctc cagctggccg
     1201 aagccggggt ctataccaag atcgacctgc ccgaccggtt cggcggggcc agcctgcccg
     1261 atgtctcgat cgtcgacctg cgccaggaaa tgccggagcg cgggcactgg ctggccccgc
     1321 ggctggtcgc ggaaatgcgg gcccggctgg accggggcga gcagacgctg ctgttcctca
     1381 accgccgcgg ctatgccccg ctcaccctgt gccggcactg cggctaccgc tttcagtgcc
     1441 ccaattgcag cgcctggctg gtcgagcacc ggctctcgcg ccggctggcc tgccaccact
     1501 gcgggcacga ggtgccgccc cccgcggcct gccccgaatg cggcgagccc gattgcctgg
     1561 tcgcctgcgg cccgggggtg gagcggatcg ccgacgaagt ggccgaagtc ctgcccgaag
     1621 cccgcgtcgc ggtggtgacc tccgacacgc tcaacagtcc ggaaaagatc agcgagttcg
     1681 tcgccgcggc cgaagagaag cggatcgacg ttatcgtcgg cacccagatc gtcaccaagg
     1741 gctaccactt tcccgacctc accttggtgg gcgtggtcga tgccgatctg ggcctggaag
     1801 gcggcgatct gcgcgcggca gagcggactt atcagcaggt cgcccaggtc gccggccggg
     1861 ccgggcgggc cgaaaagccg ggggccgtgc tgatccagac ccgccatccc gaagcggcgg
     1921 tgatcgcggc gctggccgcc ggagaccgcg atgcctttta cgaagccgag gccgaggccc
     1981 gcaaggacgc cggcgccccg ccgttcggcc gctgggccgc gatcatcgtc tccagcgagg
     2041 acgaggccga ggcccgggag gcggcccgcg cggtgggcgg cacggccccc aacctgcccg
     2101 atgtcctggt gctgggccct gcgccggctc ctttgagcct gctgcgcggc cgttaccgct
     2161 accgcctgct gatcaacgcc cgccgcaccg ccaagctgca ggatgtcatc cgcgaatggc
     2221 tcgcccccct gcaatttccg caaggcgtgc ggatcagcgt ggatatcgat ccctattcgt
     2281 tcgtttagtt gcaacgtctt gccgcaattc aagaattgcc gcagtctaat cccatgcgct
     2341 ggattcgttt ttgcttgtcg gtattgctcg cagtgcttgc gcaatgcctg gttgtccctg
     2401 ccgcccacgc caagtggcta agggccgaaa cccgcaattt cgtgatctac agctcgggga
     2461 gcgagagaga gctgcgcagg tttgccagca atctggaaat gttcgacggg ctggcccagc
     2521 ggcgcttcgg gctggagccg aacgacaaca ccaacaagct gaccatctat ttcctagact
     2581 cgcagagcga tgtctcgcgg ctggcgggtg acaaggacag gatggtcgcg ggcttctaca
     2641 gcgcccggct ggaaggcagc tttgcggtgg ccaaccgttc acgcggggac ggcatgtggg
     2701 acctcaacgg gatgaccgtg ctgttccacg aatacgccca ccacttcatg ttccggaact
     2761 tcgatttcgc ctatccggcg tggtacgtcg aaggctttgc cgaatacctt tcgaccgcca
     2821 cgttctcggc cgacggtagc tggaccatgg gcaagccggc ctttcaccgg gcctacagcc
     2881 tgcttgaagg gaagaagctg ccgatcgaga agctgctgtt cagcagcgtg tccgatctca
     2941 agtccgacga agtctcggtg ttctatggcc gcgcctggct gctggtccac atgctcaaca
     3001 tgaagccgga gcaccagggc aagctgaaga cctatttcac cgcgatccgc gaaggcaagc
     3061 cgccgcgcga ggcagccgcg ctggcctttg gcgacctgca gcagctcgac aagtcgctcg
     3121 acctctatct gcgccagcgc ctgtcctact acacctcgcc cacgccgatc cccgccgata
     3181 ccaccaccac ggtgaccgag ctggacgagg cgaccgggca actggtcgga ttccggatgc
     3241 tgcggctggt cgggcggatg gagcccaagg ccatggccgc gctgcgcgga ctggccgcgg
     3301 cgaacctagg gtcggccgag atctggcacg agctggccct ggccgagcgg caattctcag
     3361 gtcccggcga tgacaacgag gccgcgcgcc aagcccgcga gaaggccgcc gaagcggccg
     3421 tcgaccgcgc gctcgccgcc gatcccaacc acgtgcgggc caatgtcctg aaggccgata
     3481 tcctgatgac ccggctggaa gaaggccggg tgaccgagcc ggcgcggtgg agcgatgcgc
     3541 gcaagttcct gatcaccgcc aacaagtacg cggtcgacga tccgctggtg ctgttcaact
     3601 ggtacaacag ctttcgcctg caaggccgcg agccgagcgc cacggcccgg gccgcgctgg
     3661 cgcgggcctt tgtcctggcg cccgaagtga tcaacgtgcg ggtggcctat gccttcgatc
     3721 tggccgacca gcgccggttc gatgaagcgc tgggtctggt cgagtttctg gcccagcacc
     3781 cccaccacgc cgccgagggc cgcgaactgg ttgagcggat caaggaaatg cgcgaccgcg
     3841 cggcggccga caaggacagc tagcccccgt cccgcttcgc aaacaccgcc ggatcgatca
     3901 gggcgtcggt ttcgacccgc tgcagcgccc agccgccggt gggcatcgaa tcgtcgtcga
     3961 cataggattc gtaggcaaag accagtccgc gggtcaggtc cttgcggttc gagacgcaga
     4021 tggcatcgcc gacaaagccg tcaccgttgt tgcggcaggt cacggtgatt cccgcgtgct
     4081 cctgctcgct gcagcgtccg atccgccggt ttggccggca atcgagcttc gggttctgga
     4141 aatagggaac ctcgctgtat ttccacttgc cggtccgcac ggtggcccgg ctggtcagcg
     4201 catagctggc ggtgcaggtc tcggtgtcga tcgaaaggtt ggacgtcacc gtgacaaccg
     4261 ccaccgcagt ggcggtgcga tagatccgca ccgcgccctt gccgcggttc tcctgcccgg
     4321 acagcgtcac cacttttccg cgcggcgcgc aggcctcgtc cagctcttcc agttgccgct
     4381 cgtcaaagat cgaagacagc ccgagcactt cggcatagag gccgggaaca cgcggctggc
     4441 cggagggttc atagcccgcc ggcagcggcg cttcgaaggg ttcggcca
//
LOCUS       DASUDV010000388         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1426965,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000388 DASUDV010000000
VERSION     DASUDV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426965"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFV30_08440"
     CDS             complement(<1..302)
                     /locus_tag="VFV30_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX4848159.1"
                     /translation="MGRAGTSIGNRIAPLRFVAFAALLPLGFLQWQRIDPAAAWQDAL
                     AMGFDLAALVFLASLVPLLRGFSVAEMRRHSQENDANRTLVLLITTVLAAVIMAAIS"
     gene            complement(307..2268)
                     /locus_tag="VFV30_08445"
     CDS             complement(307..2268)
                     /locus_tag="VFV30_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX4848160.1"
                     /translation="MTTKTRIALAAAVFALAAPAISATLSAPMAVPLSRQVPDAADVP
                     YPGGTMRLNIDATDITRGTYRVTQTVPVAPGTKRLTLLMPQWLPGNHGPRGPMAELVD
                     IQFFAGDQKLTWKRDPVEVYAFHIDVPAGAKEVTAKYIHTSPLQPSEGRITMTQEMLN
                     LQWEKMSLYPAGHYVRRIRVIPSVTIPQGWTAAVALDGVQQQGNTLTWAETSYEILVD
                     SPIFAGKYFRKWDLGQNVTLNVIADKPEQLNAKPEHIEPHRHLVTEARLAFGANHFDR
                     YEFLLALTDRMGGIGLEHHRSSENQLEPNAFLEWDKYEWDRNLLPHEYAHSWIGKYRR
                     PARLWTPDYRQPMQDDLLWTYEGQDQFWGTVLAARSGLQGKDMVLGMLAAWAGNFATQ
                     PGRRWRSVEDTGHDPIFAARKAKPFPSLARNEDYYTEGALIWLEIDQIIREKTGGKRS
                     IDDFAKLFFGMNDGDYGQLAYELDEIVAKLNAVAPYDWAGFIETRINTPGQPAPLMGI
                     EKGGYRLVWKDEPNPFDKGRFADQKVVSLVYSLGITIDKDGKVAGTQWDSPAFNAGIV
                     PGSRIVAVNGSAYEPDAIRGAVTAAKDGAPIELLIQRGSRYQTVTIDYRGGLRYPWLE
                     RAAAGTAPTGLDLLLTPKRPQPKPKGKGK"
BASE COUNT          380 a          825 c          864 g          428 t
ORIGIN      
        1 gatatggcgg ccatgatgac ggctgccagc accgtggtga tcagcagcac cagagtgcgg
       61 ttggcgtcgt tctcttgcga atggcggcgc atctcggcga cgctgaagcc gcgcagcagc
      121 ggcaccagcg aagcgagaaa gaccagggcc gccaggtcaa agcccatggc cagggcgtcc
      181 tgccaggcgg cggccggatc gatccgctgc cactgcagga aacccagcgg cagcagcgcg
      241 gcgaaagcga cgaagcggag cggggcgatc cggttgccga tgctggttcc ggcccgcccc
      301 accgcgttac ttgcctttgc ccttgggctt gggctgcggc cgcttcgggg tgagcagcag
      361 gtcgagcccg gtcggcgcgg tgccggcggc ggcgcgttcc agccacggat agcgcagccc
      421 gccgcggtaa tcgatcgtca ccgtctggta ccggctgcca cgctggatca gcaactcgat
      481 cggcgccccg tccttggccg cggtcaccgc gccgcggatc gcatcgggtt cataggccga
      541 tccgttgacc gccacgatcc gggatcccgg cacgatcccg gcgttgaagg ccgggctgtc
      601 ccactgggtt cccgccacct tgccgtcctt gtcgatcgtg atgcccagcg aatagaccag
      661 cgacaccacc ttctggtcgg cgaagcggcc cttgtcgaag gggttgggct cgtccttcca
      721 gaccaggcgg tagccgccct tttcgatccc catcagcggg gccggctggc ccggcgtgtt
      781 gatccgggtt tcgatgaagc cagcccagtc atagggggcc acggcgttca gcttggcgac
      841 gatctcgtcc agttcatagg ccagctggcc gtaatcgccg tcgttcatgc cgaagaacag
      901 cttggcgaaa tcgtcgatgc tgcgcttgcc gccggtcttc tcgcggatga tctggtcgat
      961 ctccagccag atcagcgcgc cttcggtgta atagtcctcg ttgcgggcca ggctggggaa
     1021 gggcttggcc ttgcgcgcgg cgaagatcgg atcgtgcccg gtatcctcga ccgagcgcca
     1081 gcggcggccc ggctgggtgg cgaaattgcc ggcccaggcg gccagcatgc ccagcaccat
     1141 gtccttgccc tgcaggcccg accgcgcggc caggaccgtg ccccagaact ggtcctggcc
     1201 ctcataggtc cagagcagat cgtcctgcat cggctggcgg taatccgggg tccacagccg
     1261 cgccgggcgg cggtacttgc cgatccacga atgggcgtat tcgtggggca gcaggttgcg
     1321 gtcccattcg tacttgtccc actccaggaa ggcgttcggc tccagctggt tctcgctcga
     1381 gcggtggtgc tccagcccga tcccgcccat ccggtcggtc agcgccagca ggaactcgta
     1441 gcggtcgaaa tggttggcgc caaaggccag ccgcgcctcg gtcaccagat ggcggtgcgg
     1501 ctcgatatgc tcgggcttgg cgttcagctg ctcgggcttg tcggcgatca cgttgagcgt
     1561 gacgttctgg cccaggtccc acttgcggaa atacttgccc gcaaagatcg gggaatcgac
     1621 caggatctca tagctcgttt cggcccaggt cagggtgttg ccctgctgct gcaccccgtc
     1681 cagcgccacc gccgcggtcc agccctgggg gatcgtgacc gaggggatca cccggatccg
     1741 ccggacatag tgcccggccg gatagaggct catcttctcc cactgcaggt tgagcatttc
     1801 ctgcgtcatg gtgatccggc cctcgctggg ctgcagcggg ctggtgtgga tgtacttcgc
     1861 ggtcacctcc ttcgcgccgg ccggcacgtc gatgtggaag gcatagactt ccaccggatc
     1921 gcgcttccag gtcagcttct ggtcaccggc gaagaactgg atgtcgacca gctcggccat
     1981 cggcccgcgc gggccgtggt tgccgggcag ccactgcggc atcagcaggg tcagccgctt
     2041 ggtgccgggg gcgaccggga cggtctgggt gacgcggtag gtgccgcggg tgatgtccgt
     2101 cgcgtcgatg ttcagccgca tcgtcccgcc gggatagggc acatcggccg catcgggcac
     2161 ctggcgcgac agcggcacgg ccatcggtgc ggaaagggtt gcggagatgg ccggcgcggc
     2221 aagggcgaac acggcggcgg cgagggcgat gcgggttttc gtcgtcatgg cgtccctcat
     2281 gccgccaacc gcgcggcggc gcaaccggct ggaacctgac aaaaagggag cgcccccgtt
     2341 gccgggcaag cctggtcctg gcggaggggc caggggcgaa tccggcttca cctattgccc
     2401 gcggcggact aagcgctgcg accatgagca agaccccaca ggcgatccgc ggaacccagg
     2461 acattttcgg ccccgatgcc gaggcctttg cgcacgt
//
LOCUS       DASUDV010000389         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_167952,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000389 DASUDV010000000
VERSION     DASUDV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167952"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFV30_08450"
     CDS             <1..691
                     /locus_tag="VFV30_08450"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX4848161.1"
                     /translation="GQILLEVRRAGVFAGKALGIAGDQTANQFLVRAIREQRPEDGVL
                     SEEEKDNFERLAHARVWIIDPVDGTREYGEERADWAVHVGLAIDGVAEVGAVALPGLG
                     VVLRSDQPQPLPAPAGTPRMLVSRTRPAAEAVAVAEALGAELVPMGSAGAKAMAVVRG
                     EAEIYLHTGGQFEWDSCAPVAVARAHGLHVSRADGAPLVYNQSNTYMPDLLICRPEWA
                     EPVLKAMRALA"
     gene            complement(688..1644)
                     /gene="trxB"
                     /locus_tag="VFV30_08455"
     CDS             complement(688..1644)
                     /gene="trxB"
                     /locus_tag="VFV30_08455"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX4848162.1"
                     /translation="MMTTHTTRMLIIGSGPAGLSAAIYGARAGMQPIVVQGLQPGGQL
                     TITTDVENYPGFRDVIQGPWLMQEMQAQAEHVGTRMLWDTIVDIDLSGSPFRATGDSG
                     DVYEGDTLVIATGAQAKWLGVPGEQELSGKGVSACATCDGFFYRGKKVVVIGGGNTAV
                     EEALYLTNHSQDVTLIHRRDSLRSEKILQDRLFAHPNIKVLWNKAVDHFVGGESGLVG
                     VALKDTVTGETMTEPTDGAFVAIGHAPATELFKGKLELDGGGYIVVQPGTPKTAIPGV
                     FACGDVMDHTYRQAVTAAGTGCMAALDAERFLAERDFAASKG"
     gene            1804..>2385
                     /locus_tag="VFV30_08460"
     CDS             1804..>2385
                     /locus_tag="VFV30_08460"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX4848163.1"
                     /translation="MFKTRITEMFGIETPILMGGMTGVGYGELVAAVANAGALGFITA
                     HMFPSGEALAAEIEKTRALTDKPFGVNLTLLPSINPIPYDDYREAIIASGIKIVETAG
                     RAPTDHLPRFKENGVKVIHKCTSVRHSVSAVRKGVDVISIDGFECAGHPGEDDVGLVV
                     LLPATVDALPDVPIIASGGMADGRSLVAALALGA"
BASE COUNT          412 a          782 c          796 g          395 t
ORIGIN      
        1 cgggcagatc ctgctcgagg tgcgccgcgc cggcgtgttc gccggcaagg cgctggggat
       61 cgccggggat cagaccgcca atcagttcct ggtccgcgcg atccgcgaac agcggcccga
      121 agatggcgtc ctttccgagg aggaaaagga caacttcgag cgcctcgccc atgcacgcgt
      181 gtggatcatc gatccggtcg atggaacccg cgaatatggc gaggaacgcg ccgactgggc
      241 ggttcacgtc gggctggcca tcgatggcgt ggccgaagtg ggcgcggtgg cattgccggg
      301 acttggcgtg gtgctgcgtt ccgatcagcc gcagcccctg cccgcgcccg ccggcacgcc
      361 gcgcatgctg gtcagccgca ctcgcccggc ggccgaggcc gtggccgtgg ccgaggcgct
      421 cggggcagaa ctcgtgccga tgggcagcgc cggggccaag gcaatggcag tggtgcgcgg
      481 cgaggccgag atctatcttc acaccggcgg ccagttcgaa tgggacagct gcgcgccggt
      541 ggcggtcgcc cgcgcccacg gcctgcatgt cagccgcgcc gatggcgcac cgctggtcta
      601 caaccagagc aacacctaca tgcccgatct gctgatctgc cggccggaat gggccgagcc
      661 cgtgctcaag gcgatgcgag cgctcgccta gcccttgctg gcggcaaaat cgcgttcggc
      721 gaggaaccgt tcggcatcga gcgcggccat gcagccggtg cccgccgcgg tcaccgcctg
      781 gcgataagtg tggtccatca cgtcgccgca ggcaaagacg ccggggatcg cggtcttggg
      841 cgtgccgggc tgaaccacga tgtagccgcc gccatccagt tccagcttgc ccttgaacag
      901 ttcggtcgcg ggggcatggc cgatcgcgac gaaggcgccg tcggtcggct cggtcatggt
      961 ttcgccggtc acggtatcct tcagggcaac gccgacgagg ccgctctcgc cgccaacgaa
     1021 gtgatcaacc gccttgttcc acagcacctt gatgttggga tgggcgaaca ggcggtcctg
     1081 caggattttt tccgaacgca gcgaatcgcg gcggtggatc aaggtcacat cctgcgaatg
     1141 gttggtgaga tacagcgcct cttccaccgc agtgttgccg ccgccgatca ccaccacctt
     1201 cttgccgcga taaaagaacc cgtcgcaggt ggcgcaggcc gaaaccccct tgcccgaaag
     1261 ctcctgctcg cccggaacgc ccagccactt ggcctgggcg ccggtggcga tcaccagggt
     1321 atcaccttca tagacatcgc cgctgtcgcc agtcgcgcga aacggcgagc ccgaaaggtc
     1381 gatgtcgacg atcgtgtccc acagcatgcg ggtgccgaca tgttcggcct gggcctgcat
     1441 ttcctgcatc agccagggcc cctggatcac atcgcgaaag cccggatagt tctccacatc
     1501 ggtggtaatt gtcagctggc cgcccggctg caggccctgc accacgatcg gctgcatccc
     1561 ggcgcgcgcg ccatagatcg cggcggaaag cccggccgga ccggagccga taatcagcat
     1621 gcgggtggta tgggtggtca tgatcgatcc ctgttatgga tatgcgcctg ggcaggcctg
     1681 cgccctagcc gcgccaccac gcccgttcaa gaaacatgcg aaaaatgacc acggtttaat
     1741 tgagcggttg aaaatcccgc cagcaccggc gagactgccc tccaagctgg agaggagttg
     1801 gccatgttca agacccgcat caccgaaatg ttcggcatcg aaaccccgat cctgatgggg
     1861 gggatgaccg gggtgggcta tggcgaactg gttgccgcag tggccaatgc cggggcgctg
     1921 ggcttcatca ccgcgcacat gttcccttcg ggcgaagcac ttgccgccga gatcgagaag
     1981 acccgcgcgc tgaccgacaa gccgttcggg gtcaacctca ccctgctgcc ttcgatcaac
     2041 cccatccctt atgacgatta tcgcgaggcg atcatcgcca gcggcatcaa gatcgtcgaa
     2101 accgcgggcc gggcgccaac cgatcacctg ccgcgcttca aggaaaacgg ggtcaaggtg
     2161 atccacaaat gtacctcggt gcgccactcg gtttcggcgg tgcgcaaggg ggtcgatgtg
     2221 atctcgatcg acgggttcga atgcgccggc cacccgggcg aggacgatgt cgggctcgtg
     2281 gtgctgctgc cggccacggt cgatgccctg cccgacgtgc cgatcatcgc ttcgggcggg
     2341 atggccgatg gccgctcgct ggtggcggcg ctggcactgg gggct
//
LOCUS       DASUDV010000390         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_278210,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000390 DASUDV010000000
VERSION     DASUDV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278210"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFV30_08465"
     CDS             <1..765
                     /locus_tag="VFV30_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848164.1"
                     /translation="PAARWLDPVCPKVSGLADPAVAARVEARFRSIASAVGARLAKPG
                     CKTNLLIGFTNDADALARQINADDPRQMIELSNAWRGRLVNGRTPMRWWYSTEVRSAD
                     GAPPINAPLPWLGISMEGGGESSGGSTTNTDVGGDSGGGYTNSRRASLISSNAKRAIR
                     TATVLVDVTLAQGKDLDGVTDYAALVGLAEIRFSGEPPANSVLALFAPENPVYGLTTQ
                     DEALLKSLYRLPLDRQGRYHRGWLVNGVTKALSASE"
     gene            838..1701
                     /locus_tag="VFV30_08470"
     CDS             838..1701
                     /locus_tag="VFV30_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848165.1"
                     /translation="MLADDIVVQGQALRPEEAEARALAYVQALGVASSNKPAARWIDP
                     VCPKVTGIADPKIASRVIARIRKIAADIGARVDRAGCRGNLMVAFADDGADLARRIGA
                     ANPRLMGQVPTDWRGQLVSGTAPVRWWYSFSIRGADGKPPTTATLPWFSVELDGGNNA
                     IAANVDVGNESGGGYNIATRSSLISTNSKRAITSATVVVDVRQAEGKDLAAITDYAAL
                     VGLAEIRFVPSPAPDTVLGLFEPASAPRGLTDQDQALLKSLYSLPLDRQGRYHRGWLV
                     RDIGKSMTAAE"
     gene            1827..>2086
                     /locus_tag="VFV30_08475"
     CDS             1827..>2086
                     /locus_tag="VFV30_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4848166.1"
                     /translation="MRLNSHKPAALALLGLLAIPMSANLSAQTNNDPNIVVTGEPLPD
                     MSAMTPGPELKGVITARKGDRMKITSADGTSTVVAVNETTKI"
BASE COUNT          355 a          675 c          697 g          359 t
ORIGIN      
        1 ccggcagcgc gctggctcga tccggtctgc cccaaggtca gcgggctggc cgatccggcg
       61 gtggccgcgc gggtcgaggc gcggttccgg tcaatcgcca gcgcagtcgg cgcgcggctg
      121 gccaagccgg gctgcaagac caacctgctg atcggcttca ccaacgatgc tgatgccctc
      181 gcccgccaga tcaatgccga cgatccacgc cagatgatcg aactgagcaa tgcctggcgt
      241 gggcgtctgg tgaatggccg caccccgatg cgctggtggt attccaccga agtgcgcagc
      301 gccgacggcg cgccgccgat caacgcgccg ctgccatggc tggggatctc gatggaaggc
      361 ggcggtgaga gcagcggcgg atcgaccacc aacaccgatg tcggtggtga cagtggtggc
      421 ggctatacca attcgcggcg ggccagcctg atttcgagca acgccaagcg cgccatccgg
      481 accgcgaccg tgctggtcga tgtcaccctg gcgcagggca aggatctgga cggggtaacc
      541 gactatgccg ccctggttgg cctcgccgaa atccgctttt cgggcgaacc gccggccaat
      601 tcggtcctgg ccctgtttgc gccggaaaac ccggtctatg gccttacgac ccaggacgag
      661 gcgctgctca agtcgcttta ccgtctgccg cttgaccggc agggccgcta tcaccgtggc
      721 tggctggtca atggcgtgac caaggcgctg tccgcgtcgg agtaggagct gcagatggtg
      781 caccgcacgg cttcccgctg ccttgtcctg ggcttgttgg cagcgctgac tgccccattg
      841 ctcgccgatg acatcgtcgt gcagggtcag gcactgcgcc cggaagaggc cgaggcgcgg
      901 gcattggcct atgtccaggc gctcggggtg gccagttcaa acaagcccgc ggcgcgctgg
      961 attgatccgg tctgtccgaa ggtcaccggg attgccgatc caaagatcgc cagccgggtc
     1021 atcgcgcgga tccgcaagat tgccgccgat atcggggcca gggtcgacag ggcggggtgc
     1081 cgcggcaacc tgatggtcgc ctttgccgat gatggcgccg atctggcgcg acggatcggc
     1141 gcggccaacc cgcggctgat gggccaggtg ccgaccgact ggcgcggaca attggtcagc
     1201 ggcacggcgc cagtgcgctg gtggtacagc ttctcgatcc gcggcgccga tggcaagccg
     1261 cccaccaccg cgaccctgcc ttggttttcg gtcgagctgg acgggggcaa caatgcgatc
     1321 gcggccaatg tcgatgtcgg caacgaaagc ggcggcggtt ataacattgc cacgcgttcc
     1381 agcctgatct cgaccaattc gaagcgtgcg atcaccagcg ccaccgtggt ggtcgatgtc
     1441 cgccaggctg agggcaagga cctggccgcg atcaccgatt acgccgcgct ggtcgggctg
     1501 gccgaaatcc gtttcgtgcc cagccccgca cccgatacgg ttttggggct gtttgagcca
     1561 gccagcgcgc cgcgtggcct gaccgatcag gaccaggcgc tgctcaaatc gctctacagt
     1621 ctgccgcttg accgccaggg tcgctatcat cgcggctggc tggtgcgtga tattggcaag
     1681 agcatgactg cggctgaata gtcggcacgt cgcgcaacgg ttcatcgcag ttccgcccca
     1741 agcccccgcg caagctattg accgggcgcg tccaatgctc tggcagcgcg gttgggggcg
     1801 cagttgcgca tggaaggact gatctaatgc ggctcaattc tcacaagccg gcggctttgg
     1861 ccctgctcgg cctgctcgcc attccgatgt cggccaatct ttcggcccag accaataacg
     1921 atcccaatat cgtcgtcact ggcgagcctt tgcccgatat gagcgcgatg acgcccgggc
     1981 cggaactgaa gggcgtaatc accgcccgca agggtgaccg gatgaagatc acttctgccg
     2041 atggcaccag cacggtcgtg gcggtcaacg agacgaccaa gatcaa
//
LOCUS       DASUDV010000391         6566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_233957,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000391 DASUDV010000000
VERSION     DASUDV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233957"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1106)
                     /locus_tag="VFV30_08480"
     CDS             complement(189..1106)
                     /locus_tag="VFV30_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848167.1"
                     /translation="MARLIPLALALAAAAGLSPLLGAPAPKSALDCPAPPGWSDVLKQ
                     APRFVVFGELHGTEQPPQLIGQLVCAEALKGKRVLLAVELDSRFDDRLQAAWKLPSGQ
                     FEPALAKLDWEGRQDGLASKAMFDLLVRMHLLKERGLPVAITAFNGAKSKEQFEKFSN
                     LPGQGGHEATQAENIANAATAGAPDLVIVLVGNFHARKAPIARRGVAWDPMARYLSGY
                     GKVVSLDIRYGPGTSWNCLMKPDVKLAPGQRVGPEHINCGPSPTPGNTKGTGPVRIAL
                     PATDPAASAEGFDGTFWVGPVTASPPAFP"
     gene            1354..1530
                     /locus_tag="VFV30_08485"
     CDS             1354..1530
                     /locus_tag="VFV30_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEX4848168.1"
                     /translation="MTKPPIPGPSHTDGPIGLADPVRAALEEAIAKLRFGVVQLTVHE
                     GRVVQLEVTEKRRF"
     gene            1652..4096
                     /locus_tag="VFV30_08490"
     CDS             1652..4096
                     /locus_tag="VFV30_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848169.1"
                     /translation="MHSFVRLSLLASAALVALPAAAEEAAPADQEVSSAGTGAAAPEE
                     EILVTARRRAEASQEVPLAISVVGGEHLDNTGAFNVGRLQQLTPTLQFYTSNPRNSAA
                     NIRGLGAPFGLTNDGIEQGVGIYVDDVYYSRAASSTFDFLDVARIEVLRGPQGTLYGK
                     NTTAGALNITSRQPTFTFEGRAEVSIGNLGFKQAKAAVSGPLSNNLAARLAVSATGRK
                     GTVFNRATGNWINEQDNLGLRAQLLWQPAEGVDVTLAGDYSVQDPECCGQIYVGTGAT
                     QRPLNRQFAALAALQGYTVPSTNPFDRVTDLDANLNAGNKIGGVSLRVKWDAGPGTLT
                     SVTAWRFWDWKPANDRDFIGLPITTASNNPSQQHQYTQEFRYARDGDRVDFVAGLFGF
                     HQTVRTQGLQIQGPAASRWLLNPSSALSLNPAVLNGLTANNDIRLDNTSAAVFGQASW
                     KITDALTLQPGVRINYDKKKGLYASVVTGTASDGTRQLVLFSGAYAADPWIAAQRGVL
                     SQQRFEPEFSDWNFSYDINVSYKLSGDVLVYGTYAKTFKSGGINLNGVPNDAAGNPLL
                     AAGTVKPESFNHYEAGLKAQFWDRKVTFNLSAYRTDIGNFQALVTNGQLGVLRGYLAN
                     AEKVRTQGLEFDLSVRPSARFNAYLNGAYTDATYVRFVDAPCPPELSGGGTGTPLAAP
                     GVPGNSPANCDISGQRLPGVSKWAFSWGAEGNLPASLLGQDGEVYLGVDGSYRSRFSS
                     NPTPSAYTWVDGYALTNLRLGFRTDKGLNVFGWVRNAFNVNYFEQLAVPSGNTGLIVG
                     SPGDPRTWGGTIKIEF"
     gene            4300..4779
                     /locus_tag="VFV30_08495"
     CDS             4300..4779
                     /locus_tag="VFV30_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848170.1"
                     /translation="MQAKRHTLRWSLAAVLGCLAMPGGQTAVFAQSEAEAKQLRAQCD
                     AGDPDACLNLQALKDNAALAEACAKGFANGCYNLGVRYRIGDGPLQPDLARAADLFDK
                     ACQLGHAYGCNDLGTLYLQGAGVTKSKAKAADCFRKALAIDPGHAAARKNLAAAEAK"
     gene            complement(4855..>6566)
                     /locus_tag="VFV30_08500"
     CDS             complement(4855..>6566)
                     /locus_tag="VFV30_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4848171.1"
                     /translation="FGNVAQLHTVKGPGMARTQISVEPEPLRGTYLPGDGTMVVQKDK
                     GGDEFFQLYTLKNGKLTLLTDGKSRNGLNAVSHDGKWIAYSSTRRNGRDTDLYIMDPR
                     NPSTDRMVKQVEGGGWAIADFAPGNKQAVVANYISVTKTNLYWLDLETGAMTPIGDHK
                     KAIAYGGGSFAPDGTLWVTSDEGSDFQRLGTLDPKTGKFTPKSPEPKWDIEGFDIAED
                     GSFIAYTVNEAGVSKLRLMDPKTGAVREVPGLPAGTIGGVGIAPWGTIGFTISSAKVP
                     ADAFSVDPKTLEVTRWTMSETGGLDPSVNVEPEFVEVKSFDGELVSGFLYRPDPKKFP
                     GPRPLIFNIHGGPEGQSTPGFRGRTNYLINELGVAVFYPNVRGSTGFGKRFVSLDNGP
                     FLRENSVKDIGAFLDRLEADPAIDKNRIAVQGGSYGGYMCYATAIRYGGRFKAADCIV
                     AISNFVTFLENTQSYRRDLRRVEYGDERDPKQRAKLLEISPLTKIDQLTIPLLVVTGA
                     NDPRVPKSEADQAVAAVRAKGRPAWHLVGMNEGHGFAKKENADYQFWTELLFWKTHLL
                     GEK"
BASE COUNT         1088 a         2149 c         2235 g         1094 t
ORIGIN      
        1 ccggttcggg gccttcgtag aggatcttgg ccttgccttc gtagatctgg cggcgacggg
       61 acatgggagc acttccttgg gcagagaaac ggcaaacccc ggcaggtgcg ggtcggaagg
      121 gactcgcgcg gccggggcgg acgcctgcgc cttacgcgat ttggcgcgcg ggcgcaacgt
      181 gggggcgctc agggaaatgc cggaggggac gcggtgaccg ggccgaccca gaaggtgccg
      241 tcaaagccct ccgcgctggc ggccggatcg gtggcgggaa gcgcgatccg aacggggcct
      301 gtgcctttgg tgttgccggg cgtcgggctg ggtccgcaat tgatatgctc gggcccgacc
      361 ctctggccgg gggcaagctt gacgtcgggc ttcatcaggc agttccagct ggttcccggg
      421 ccatagcgga tgtcgagcga gaccaccttg ccatagcccg agaggtaccg ggccatcgga
      481 tcccaggcca ccccgcggcg cgcgatcggg gctttgcggg cgtggaagtt gccgaccagc
      541 acgatcacca ggtccggcgc gccggccgtg gccgcgttgg cgatattctc cgcctgcgtc
      601 gcctcgtgcc cgccctgacc cggcaggttg gaaaactttt cgaattgctc cttgctcttc
      661 gcgccgttga aggcggtgat cgcaaccggc agcccccgct ctttcagcag gtgcatccgg
      721 accagcaggt cgaacatcgc tttgctggcc aggccgtcct gccgcccctc ccagtccagt
      781 ttggcgaggg cgggttcgaa ctgtccggag ggcagtttcc aggcggcctg aagcctgtcg
      841 tcgaagcgcg aatcgagctc gaccgctagc agcacgcgct tgcccttcag cgcttcggcg
      901 cagaccagct ggccgatcag ctgcggcggc tgctcggtgc cgtgcagctc tccgaagacc
      961 acgaaccgcg gcgcctgctt gaggacatcg gaccacccgg gcggggccgg gcagtccagc
     1021 gcggacttcg gcgctggcgc gcccagcagc ggggaaagac cggcggcggc cgccagggcc
     1081 agggcgagcg ggatcagtct ggccatgcac gaacctccac gaaggtacag tgatcctttc
     1141 acgggaaggg caaaggggca agtgcctgat tttgaaggac cggcggccgt ggtcatgcgg
     1201 cacgatgcgg gtgcggcagt ggccatgcgc aaccccgtcc cggaccggcc ccgcagcttg
     1261 ccgcaagaaa gctgcgcttg gtcgagcaca agcgaatctg cgcttgtcta tgcaaaaggt
     1321 agagttatcc cttggcgcca aggagaccaa gccgtgacca agcccccgat tcccggcccg
     1381 agccacaccg atggacccat cggactggcc gatccggtgc gtgcggcgct ggaggaagcc
     1441 atcgccaagc tccgcttcgg cgtggtccag ctgacggttc acgaagggcg cgtggtccag
     1501 cttgaagtga ccgaaaagcg ccgtttctaa gatgtcccgg cacgggaccg gggccggcgg
     1561 cgcatgtccg gccccgcacc gcgccaccca aacccatcgt tgcagaccgg accaccggat
     1621 gcggccaatc accctgacaa aggaattgga aatgcattcg ttcgtccgcc tgtccctgct
     1681 tgcttccgcc gcgctggtgg cgctgcccgc tgccgccgaa gaggccgcgc cggccgatca
     1741 ggaggtctcg tccgccggca ccggtgccgc cgcacccgag gaggaaatcc tcgtcacggc
     1801 ccggcgccgc gccgaagcca gccaggaggt gccgctggcc atttcggtgg tcggcggaga
     1861 gcacctcgac aacaccgggg cgttcaacgt cgggcggctg cagcagctga ccccgaccct
     1921 gcagttctac acttccaacc cgcgcaacag cgccgccaac atccgcgggc tgggggcgcc
     1981 gttcgggctg accaacgacg ggatcgaaca gggcgtcggg atctacgttg acgatgtcta
     2041 ttattcgcgc gcggccagct cgaccttcga tttcctcgac gtcgcccgga tcgaggtgct
     2101 gcgcgggccg cagggcacgc tctatggcaa gaacaccacg gccggggcgc tcaacatcac
     2161 cagccgccag ccgaccttca ccttcgaagg ccgggccgag gtttcgatcg gcaacctggg
     2221 cttcaagcag gccaaggcgg cggtttcggg gccgctgtcc aacaatctgg cggcgcggct
     2281 ggcggtttcg gccaccgggc gcaagggcac ggtgttcaac cgcgcgaccg gcaactggat
     2341 caacgagcag gacaacttgg gcctgcgcgc gcagctgctg tggcagccgg ccgagggagt
     2401 cgatgtgacc ctggccgggg actattcggt gcaggatccg gaatgctgcg gccagatcta
     2461 tgtcggcacc ggcgccaccc agcgcccgct gaaccgccag tttgcggcgc tggcggcgct
     2521 gcagggctat accgtgccca gcaccaatcc gtttgaccgg gtgaccgatc tggatgccaa
     2581 cctcaacgcc gggaacaaga ttggcggggt cagcctgcgg gtgaagtggg acgccggacc
     2641 gggcaccctg acctcggtaa cggcctggcg gttctgggac tggaagccgg ccaacgaccg
     2701 cgatttcatc ggcctgccga tcaccaccgc gtcgaacaat ccctcgcagc agcaccagta
     2761 cacccaggaa ttccgctatg cgcgggacgg ggaccgggtc gatttcgtgg ccgggctgtt
     2821 cgggttccac cagacggtgc ggacccaggg cttgcagatc cagggcccgg cggccagccg
     2881 ctggctgctc aatccctcca gcgcgctgtc gctcaacccg gcggtgctga acgggctgac
     2941 cgccaacaac gacatccggc tcgacaacac cagcgcggcg gtgttcggcc aggccagctg
     3001 gaagatcacc gatgcgctga ccctgcagcc cggggtgcgg atcaactatg acaagaagaa
     3061 ggggctctat gcctcggtcg tcaccggcac cgcatcggac gggacccggc agctcgtcct
     3121 gttcagcggg gcctatgccg ccgacccgtg gatcgccgcc cagcgcgggg tgctgagcca
     3181 gcagcggttc gagccggaat tcagcgactg gaacttcagc tacgatatca acgtcagcta
     3241 caagctgagc ggcgatgtgc tggtctatgg aacctatgcc aagaccttca agtcgggcgg
     3301 gatcaacctc aacggggtgc ccaacgatgc cgcgggcaat cccctgctgg cagcgggaac
     3361 ggtgaagccg gaatcgttca accattatga agccgggctc aaggcgcagt tctgggaccg
     3421 caaggtcacc ttcaacctgt ccgcctaccg caccgacatc ggcaatttcc aggcgctggt
     3481 taccaacggc cagttgggcg tgctgcgcgg ctatctggcc aatgccgaaa aggtccgcac
     3541 ccaggggctg gagtttgacc tgtcggttcg gcccagcgcc cgcttcaacg cctatctgaa
     3601 cggtgcctac accgatgcca cctatgtccg gtttgtcgat gcgccgtgcc cgcccgagct
     3661 ttcgggtggc ggcaccggca cgccgctggc cgcgccgggc gtacccggca acagcccggc
     3721 caattgcgac atttccggac agcggctgcc cggcgtgtcg aagtgggcgt tctcgtgggg
     3781 ggcggaaggc aacctgccgg cgagtctgct gggccaggac ggcgaagtct acctcggcgt
     3841 ggatggcagc taccgaagcc gcttctcctc caacccgacg ccttcggcct acacctgggt
     3901 cgatggctac gcgctgacca acctgcggct ggggttccgg accgacaagg ggctgaacgt
     3961 ctttggctgg gtccgcaacg ccttcaacgt caactatttc gaacagctgg ccgtgccgtc
     4021 cggcaacacc ggcctgatcg tcggatcgcc gggtgatccg cgcacctggg gcggaacaat
     4081 caagatcgaa ttctgaccgc cgggaagacc tctcctccca atcccctcgc ggccagggtc
     4141 cggggcgccc tttcattcgg ggcagggccg gcagggcgga gccaccccgc ggtggctccg
     4201 ccttttcatg cgcccgccga acaggctgtt gccacgcccg aagggcttgc aggatatccg
     4261 ccaaagctgg caaacagggc ccgaaacggg agagtcacca tgcaagcaaa acggcacacc
     4321 cttcgttggt ctctggcggc cgtgctgggc tgtttggcca tgccgggcgg ccagaccgcc
     4381 gtgtttgcgc aaagcgaggc cgaggcgaag cagctgcgcg cccagtgcga tgcgggcgat
     4441 ccggatgcct gcctcaacct gcaggcgctg aaggacaacg cggcgctggc cgaagcctgc
     4501 gcgaagggat ttgcaaacgg gtgctacaac ctgggggtcc gctaccggat cggggatggg
     4561 ccgctgcaac cagacctggc ccgcgcggcg gacctgttcg acaaagcctg tcagcttggt
     4621 cacgcctatg gctgcaacga tctgggcacc ctttacctgc agggtgccgg tgtcacgaag
     4681 agcaaggcca aggcggccga ctgtttccgg aaagcgctgg cgatcgatcc gggccacgcc
     4741 gccgcgcgca agaacctggc ggcggccgaa gcgaaataac cccgccgcgt ttccgccacg
     4801 cccgccttcc tgggaagggg gcgttgacgg ttcgcagcgg ggcttcgggg ggttctactt
     4861 ctcgcccagc aggtgggtct tccagaacag cagttcggtc cagaactggt agtcggcgtt
     4921 ctccttcttg gcgaagccgt ggccttcgtt catgccgacc agatgccagg ccgggcggcc
     4981 cttggcgcgg accgcggcga cggcctggtc ggcctcgctc ttgggcacgc gcggatcgtt
     5041 ggcgccggtc accaccagca gcggaatggt cagctggtcg atcttggtca gcgggctgat
     5101 ttccagcagc ttggcgcgct gcttgggatc gcgctcgtcg ccgtattcca cccggcgcag
     5161 gtcgcggcgg taggactggg tgttttccag gaaggtgacg aagttcgaaa tcgcgacgat
     5221 gcagtcggcc gccttgaaac gcccgccata acggatcgcg gtggcatagc acatgtagcc
     5281 gccataggac ccgccctgaa ccgcgatgcg gttcttgtcg atcgccggat cggcttccag
     5341 ccggtcaagg aaagcgccga tgtccttcac cgaattctcg cgcaggaacg gcccgttatc
     5401 gaggctgacg aagcgcttgc cgaacccggt cgagccgcgg acgttgggat agaacaccgc
     5461 cacgcccagt tcgttgatca ggtagttggt ccgcccgcgg aagccggggg tggactggcc
     5521 ttcgggcccg ccgtggatgt tgaagatcag cgggcgcggc ccggggaact tcttcggatc
     5581 ggggcggtag aggaacccgc tgaccagctc gccgtcaaag ctcttcacct cgacgaattc
     5641 gggttcgacg ttgaccgagg ggtccagccc gccggtttcg ctcatcgtcc agcgggtcac
     5701 ttccagggtc ttgggatcga ccgagaaggc atcggccggg accttggccg aggagatcgt
     5761 gaagccgatc gtgccccacg gggcaatgcc gacgccgccg atggtgcccg ccggcaggcc
     5821 gggcacttcg cgcaccgcgc cggtcttggg atccatcagc cgcagcttgg agacgccagc
     5881 ctcgttgacc gtgtaggcga taaagctgcc gtcctcggca atgtcgaagc cttcgatgtc
     5941 ccacttgggc tcggggctct tgggtgtgaa cttgccggtc ttgggatcga gcgtgccgag
     6001 ccgctggaaa tcgctgccct cgtcactggt gacccacagg gtgccgtcgg gggcgaagga
     6061 accgccgcca taggcaatcg ccttcttgtg atcgccgatc ggggtcatcg ccccggtttc
     6121 caggtcgagc cagtagaggt tggtcttggt caccgagatg tagttggcga ccacggcctg
     6181 cttgttgccg ggggcaaagt cggcaatcgc ccagccgccg ccttcgacct gcttgaccat
     6241 gcggtcggtc gaggggttgc gcgggtccat gatgtaaagg tcggtgtcgc ggccgttgcg
     6301 gcgggtggag ctgtaggcga tccacttgcc gtcgtggctg accgcgttga ggccgttgcg
     6361 gctcttgccg tcggtcagca gggtcagctt gccgttcttc agggtgtaga gctggaagaa
     6421 ctcgtccccg cccttgtcct tctgcaccac catggtgcca tcgcccggca gataggtgcc
     6481 gcgcagcggt tcgggttcaa cgctgatctg ggtgcgggcc atgcccgggc ccttgacggt
     6541 gtggagctgg gccacgttgc caaagc
//
LOCUS       DASUDV010000392         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1471097,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000392 DASUDV010000000
VERSION     DASUDV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471097"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="scpB"
                     /locus_tag="VFV30_08505"
     CDS             <1..605
                     /gene="scpB"
                     /locus_tag="VFV30_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX4848172.1"
                     /translation="EATLFAAEEPMSAEAISVHLGGANVKEALAELQALYAERGVQLV
                     ERGKRWHFQTAPDLAHLLRREREEVRRLSRAATEVLAIVAYHEPVSRAEIESIRGVQT
                     AKGTLDVLMEAGWVRIVGRREVPGRPVIYATTPDFLTHFGLESRRDLPGIDELRAAGL
                     LDPVEDALAEAMETAGEAAAADEEAETEAESDEPGRESAD"
     gene            703..954
                     /locus_tag="VFV30_08510"
     CDS             703..954
                     /locus_tag="VFV30_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX4848173.1"
                     /translation="MGFGSFWHWIIVLIIVLVLFGRGRVSEIMGDFGKGIKSFKQGMT
                     EGEEVKPQAPASQIPPPPPANQATLNGESVVDAADTKQP"
     gene            982..1365
                     /gene="tatB"
                     /locus_tag="VFV30_08515"
     CDS             982..1365
                     /gene="tatB"
                     /locus_tag="VFV30_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX4848174.1"
                     /translation="MFDVGASELLLIVVVAVVAIGPKDLPLALRTAGRWMGKIRRVSG
                     HFRAGVETMIREAELEEMEKKWREQNEAIMAANPYNPSADAALVGQDPGTTPAAAAEA
                     AVPAPELAPEDTAPAKPKRSRKKAS"
     gene            1362..2201
                     /gene="tatC"
                     /locus_tag="VFV30_08520"
     CDS             1362..2201
                     /gene="tatC"
                     /locus_tag="VFV30_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX4848175.1"
                     /translation="MSVFDVKDIDESQAPLLDHLIELRARLLRCVYAMGLGFAVSFYF
                     ADDIFGFLVRPLTEAFPPGEGKLIYTQLYEAFFVELKVALFSAFFLSFPIIANQLWAF
                     VAPGLYAKEKKAFLPFLLLTPVLFTLGAALAYYVVMPTAFKFFLTFEGDKGGLKMEAL
                     PGTGNYLSLVMQFILAFGISFLLPILLLLLNRAGMVSRAQLVAARRYVIVGMAILAAI
                     VTPPDVVSQLLLLIPLMLLFESALFVMLLLERQDAKEAVAAEAPANPVEPAPPSQDLM
                     GPL"
     gene            complement(2185..>2665)
                     /locus_tag="VFV30_08525"
     CDS             complement(2185..>2665)
                     /locus_tag="VFV30_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4848176.1"
                     /translation="KHGIIASMQPVHQTSDREMAEKRLGPGRLAGAYAWKSVQASGTR
                     IAFGSDVPVELADPFAGMAAAISRTGPDGAPPGGWQPQERVTREEAFAGYTVNGAYAG
                     FAEGRFGRLVVGERADFLFVDRDIFLATPAELRGTQVQEVWVNGRKVWQAPAVKAGP"
BASE COUNT          429 a          876 c          898 g          462 t
ORIGIN      
        1 tcgaggcgac gctgttcgcc gccgaggagc cgatgagcgc cgaggcaatc tcggtccatc
       61 tcggcggggc gaacgtgaag gaagcgctgg ccgaattgca ggcgctttat gccgaacgcg
      121 gcgtgcaact ggtcgagcgc ggcaagcggt ggcatttcca gaccgcgccc gatcttgccc
      181 acctgctgcg gcgagagcgc gaggaagtgc gccggctgag ccgcgccgcc accgaagtcc
      241 tggccattgt cgcctatcac gagccggtca gccgggccga gatcgaatcg atccgcggcg
      301 tccagaccgc caagggcacg ctcgacgtgc tgatggaagc cggctgggtg cggatcgtcg
      361 gccgccgcga agtgccgggc cgcccggtga tctatgccac cacgcccgat ttcctgaccc
      421 acttcggcct cgaaagccgc cgcgacctgc cggggatcga cgaactgcgc gccgccgggc
      481 tgctcgatcc ggtcgaggat gcgctggccg aagcgatgga gacggccgga gaggcggcgg
      541 cggccgacga ggaggccgaa accgaggcag agagcgacga accgggccgg gaatcggcgg
      601 actgactcgc ataaccgcag caaatgccct acatgacagg gcgaggggcg ttgtgcctcg
      661 cggacggcaa agcgccgtct gcctgtttca ggagtgacga caatgggttt cgggtctttc
      721 tggcactgga tcatcgtgct gatcatcgtg ctggtgctgt tcgggcgtgg ccgcgtgtcg
      781 gaaatcatgg gcgatttcgg caagggcatc aaaagcttca agcagggcat gaccgaaggc
      841 gaagaggtca agccgcaggc tccggccagc cagatcccgc cgccgccgcc cgccaaccag
      901 gccacgctca acggcgagtc ggtggtcgac gccgcggaca cgaagcagcc ctaaccgggc
      961 ggacagcggc ggaaccaggc catgtttgat gtcggcgcgt ccgaattgct gctgatcgtg
     1021 gttgtcgcgg tcgtggcgat cggacccaag gatcttccgc ttgcgctgcg cacggcgggg
     1081 cgctggatgg gcaagatccg gcgcgtctcc ggccatttcc gtgccggggt cgaaaccatg
     1141 atccgcgagg cagagctgga ggaaatggaa aagaagtggc gcgagcagaa cgaggcgatc
     1201 atggcggcga acccgtacaa tccctcggcc gatgccgcgc tggtcgggca ggatcccggc
     1261 accactcccg ccgccgccgc tgaagccgcc gtgccggctc cggaactggc gccggaagac
     1321 actgccccgg ccaagcccaa gcgcagccgg aagaaggcct cgtgagcgtg ttcgacgtca
     1381 aggatatcga cgagagccag gccccgctgc tcgaccatct gatcgagctg cgcgcgcggt
     1441 tgctgcgctg cgtctatgcc atggggctgg gctttgcggt cagcttctac ttcgccgacg
     1501 atatcttcgg tttcctggtt cgcccgctga ccgaggcttt cccccccggt gagggcaagc
     1561 tgatctacac ccagctctat gaagcgttct tcgtcgagct taaagtggcg ctgttttccg
     1621 cctttttcct gtcgtttccg atcatcgcca accagctgtg ggcctttgtc gcgccagggc
     1681 tttatgccaa ggaaaagaag gccttcctgc cgttcctgct gctgaccccg gtgctgttca
     1741 cgctgggcgc ggcgctggcc tattacgttg tcatgcccac ggccttcaaa ttcttcctga
     1801 cctttgaagg cgacaagggc gggctgaaaa tggaggcgct gcccggcacc ggcaattacc
     1861 tgtcgctggt gatgcagttc atcctggcct tcgggatcag tttcctgctg ccgatcctgc
     1921 tgttgctgct caaccgcgcc ggcatggtca gccgcgcgca gttggtcgcc gcgcggcgct
     1981 atgtgatcgt cggcatggcg atcctggccg cgatcgtcac cccgcccgat gtcgtctcgc
     2041 agctgctgct gctgatcccg ctgatgttgc tgttcgaaag cgcgctgttc gtgatgttgc
     2101 tgctggagcg gcaggacgcg aaggaagcgg ttgccgccga agccccggcc aatccggtgg
     2161 agcctgcgcc cccgtctcag gaccttatgg gcccgctttg accgcgggcg cctgccacac
     2221 cttgcgcccg ttcacccaca cttcctgaac ctgggtgccg cgcagttcgg ccggggtggc
     2281 caggaagatg tcgcggtcca cgaacaggaa gtcggcgcgt tcgcccacca ccagccggcc
     2341 aaagcggcct tcggcaaagc cggcataggc gccgttgacg gtgtagccgg caaaggcctc
     2401 ttcgcgggtc acccgttcct gcggctgcca tccgcccggg ggggcgccgt ccggcccggt
     2461 gcggctgatc gcggcggcca tgccggcaaa gggatcggcc agttccaccg gcacgtccga
     2521 tccaaaggcg atccgcgtgc cgctggcttg aaccgatttc cacgcatagg ccccggccag
     2581 ccggcccggc cccagccgct tttccgccat ctcgcggtcg ctggtctggt gcacgggctg
     2641 catcgaggcg atgatcccgt gcttg
//
LOCUS       DASUDV010000393         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1139827,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000393 DASUDV010000000
VERSION     DASUDV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139827"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..975
                     /locus_tag="VFV30_08530"
     CDS             244..975
                     /locus_tag="VFV30_08530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4848177.1"
                     /translation="MALITGASSGFGAHFARLLVREGARVVLGARRTDRIEALAQELG
                     EAALAVPLDVTDEASLIAAFDAGEVRFGTIDTVIANAGIVATGRAIDLPLADVAGVVN
                     TNFIGLYLTAREGARRMIKAGFKTHGRGRVVLIGSITAELTAQGDAAYAATKAGVAHL
                     GRQFAREWVRQGINVNTIQPGYIQTEIADDWFETEGGKAQIAAFHRKRLCPIEALDAP
                     LLLLASDASAHITGATLTVDDGQVL"
     gene            975..>2113
                     /locus_tag="VFV30_08535"
     CDS             975..>2113
                     /locus_tag="VFV30_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX4848178.1"
                     /translation="MDDNTPVIIGVGQVSERVGEPGYLERSPMDLAGAALKAALADAR
                     GRRSVAKAIDTLVGVRQFEQSATRYSAPVGCANNVARAIARRVGANPVRAIHEVVGGD
                     RPQRLIGELAAEIAAGRSKVAAIAGSEALSTMRALMARGETRDWSERRRGSQEDRGPA
                     YDGMVDATALKHGVGAPITAYPIAENVRRERLGLSLDQYRLEIGRLFAPFTRAAAANP
                     HSAAPVERSAEELATLNDRNRLIAEPYGRLVVARDQVNQGAAIVLASVAEARRLGVPE
                     DRWVYVHGVADCAEPTLFSRASMEASPAAVSAISTALEVAGIGWKDLGAIDLYSCFAI
                     PVFNLLDAFSLDRDDPRGWTLTGGLPFFGGAGNNYSSHAIAEAVAR"
BASE COUNT          354 a          672 c          736 g          351 t
ORIGIN      
        1 caggattcgc tgcgccggtt cagttcagct gtctgaaaaa aggagagcgg caggcattgg
       61 cgccggccgc tccaggtggg gagaaatcga ccgggaggaa accgtgagtc tccgcccgtt
      121 cgctgccgac tttctgcccc acgcgccgaa caatccttga ccgcgatcaa acttttgcaa
      181 gaacagttgc aaagacgaac tttgagggca gctcgaatca tgcacaaggc gatggacgga
      241 agagtggcat tgatcaccgg ggcctcatcc ggcttcggcg cgcactttgc ccggttgctg
      301 gtgcgtgaag gggcgcgggt ggttctgggc gcgcggcgca ccgaccggat cgaggcgctg
      361 gcgcaagagc tgggcgaagc ggcgctggcg gttccgctcg acgtgaccga cgaagcctcg
      421 ctgatcgcgg ccttcgatgc gggcgaggtg cggttcggca cgatcgacac ggtgatcgcc
      481 aatgccggga tcgttgccac cgggcgcgcg atcgatctgc cgctggccga tgtcgcgggg
      541 gtggtgaaca ccaatttcat cggcctctac ctcaccgcgc gcgaaggagc gcggcgaatg
      601 atcaaggccg gcttcaagac ccatggccgc ggccgggtgg tgctgatcgg ttcgatcacc
      661 gccgaactga ccgcgcaggg cgatgccgcc tatgccgcga ccaaggctgg cgtggcgcac
      721 ctgggccgcc agttcgcgcg cgaatgggtg cgccagggga tcaacgtcaa cacgatccag
      781 cccggctata tccagaccga gatcgccgac gactggttcg agaccgaagg cggcaaggcg
      841 cagatcgccg cgttccaccg caagcggctc tgcccgatcg aggcgctgga tgcgccgctg
      901 ctgctgctgg catccgatgc ttcggcgcat atcaccgggg caaccctgac agtcgatgac
      961 gggcaggtgc tgtaatggac gacaataccc cggtcatcat cggcgtcggc caggtttccg
     1021 agcgggtcgg cgaacctggc tatctcgaac gttcgccaat ggatctggcg ggtgccgcat
     1081 tgaaagcggc gcttgccgat gcccgcggcc ggcgcagcgt ggccaaggcg atcgataccc
     1141 tggttggcgt gcgccagttc gaacaatcgg caacgcgcta ttccgcgccg gtcggctgtg
     1201 ccaacaatgt ggcgcgggcg attgcccggc gggttggcgc caatccggtt cgcgccatcc
     1261 acgaagtggt gggcggcgat cgtccgcagc ggctgatcgg cgagctggcg gcggagatcg
     1321 cggcggggcg cagcaaggtc gcggcgatcg ccgggtccga ggcgctttcg acgatgcgcg
     1381 cgctgatggc gcggggcgaa acccgcgact ggtccgagcg ccgccgcggc agccaggaag
     1441 atcgcggccc ggcctatgat ggcatggtcg atgccaccgc gcttaagcac ggagtgggag
     1501 cgccgatcac cgcctatccg attgccgaaa acgtccggcg cgagcggctg gggttgagcc
     1561 tcgatcaata tcgcctggaa atcgggcggc tgttcgcacc ctttaccaga gcggcggcag
     1621 ccaacccgca cagcgcggct ccggtcgagc gcagtgccga ggaactggca accctcaacg
     1681 accgcaaccg gctgatcgcc gaaccctatg gccggctggt ggtggcgcgc gaccaggtca
     1741 accagggcgc ggcaattgtt ctcgccagcg tggccgaggc gcgccggctg ggcgtgcccg
     1801 aggatcgctg ggtctatgtc cacggtgtcg cggactgcgc cgaacccacg ctcttctcgc
     1861 gtgccagcat ggaagccagt ccggccgccg tatcggcgat ctcaaccgcg cttgaggttg
     1921 ctggaatcgg ctggaaggat cttggtgcga tcgatctcta ttcgtgcttc gccattcccg
     1981 tcttcaacct gctcgatgcc ttctcgctcg atcgcgacga tccgcgcggc tggactttga
     2041 ccggcggtct gccgttcttc ggcggggccg ggaacaacta ttccagccat gccattgccg
     2101 aagccgtggc gcg
//
LOCUS       DASUDV010000394         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_79519,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000394 DASUDV010000000
VERSION     DASUDV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79519"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFV30_08540"
     CDS             complement(<1..356)
                     /locus_tag="VFV30_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848179.1"
                     /translation="MTGPDLARAAEGLVGTSFRLHGRDPAFGLDCIGLLGAALARAGR
                     PAELPTGYTLRLRSLEGWLPHPRSLGFGVAERPFVPGDVVLLAPGPAQFHLAIAAADG
                     AWIHAHAGLRRVVREPG"
     gene            complement(353..1174)
                     /locus_tag="VFV30_08545"
     CDS             complement(353..1174)
                     /locus_tag="VFV30_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEX4848180.1"
                     /translation="MSRVWFSGELETVATFWRVIRRDGVTLGFTTHDRDLWFDHVLHL
                     ATPGMVPSAIRRSADFEPDSAEVEGALSHDSISASDLAQGRFDGARVLIGLVDWESFE
                     QHVIYRGTIGAVNEQAGSFTAALQSRKLELSRDPIPRTSPSCRASFCGPGCGLSNARF
                     THEATLTGFSLASNAATVATTVPLANVVGGTLRWLDGPYCGLEAVILGATGGALVLDL
                     PLDQAPSPGCRLMLREGCDRRLETCANRFANALNFQGEPHLPGNDLVTRYPSPVQ"
     gene            complement(1171..>2609)
                     /locus_tag="VFV30_08550"
     CDS             complement(1171..>2609)
                     /locus_tag="VFV30_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEX4848181.1"
                     /translation="SYELLAQHCPDAWKQRAFNGDPALTGWDPPSSLLSPANSAAMAW
                     LQAVGSAFAGLMKDAAVPVRFQIGEPWWWIMADGRPCLYDDAAKAAFGGSPVEIADLS
                     QPLNAAQKALLDAAGALLAASTAALRDAVRAVAAPQVAEVLLLTFLPGVLDPAMPEVR
                     RANLPTGWAAPAFDRLQCEDYDWLTQGAEAYRRSAYAEIDQRLGYPPLQQDYLAGFVL
                     NPADRHLWRRIDAGLDEAAQRQAHERFVWALPQVCRDGYLRLPSPSEEDTMLAFDDLS
                     YPLALGRDAMVIPEFSTSVSVTASGFERRNSLWADARLRFDVGPGIRSEAELGTLIAF
                     FRARRGAARGFRLRDPSDCSSNGMTGAPTHADQLLGTGDGTRASFALVKRYGSGDAEQ
                     LRRITRPVAGSIVVSIDGVASTAWTLDPLGVIRFAAPPAPGKAVRAGFLFDVPVRFAE
                     DKLEVAGAVFAAGEAPSVPVIEIREASA"
BASE COUNT          454 a          885 c          913 g          357 t
ORIGIN      
        1 ccgggctcgc gcaccacccg ccgcaatccg gcatgggcgt ggatccaggc tccgtccgcc
       61 gctgcaatcg cgaggtggaa ttgcgctggc ccgggcgcca gcagcaccac gtcgcccggg
      121 acaaaggggc gctcggcaac gccaaagcca agcgagcgcg ggtggggcag ccagccttcg
      181 agcgagcgca atcgcagcgt atagccggtg ggcagctcag ccggtcgtcc ggcccgcgcc
      241 agcgcagccc ccagcaggcc gatgcaatcg agcccgaagg ccggatcgcg gccgtgcagc
      301 cgaaacgacg tgccaaccag cccttcagcg gcgcgggcca gatcgggccc ggtcattgca
      361 ccggcgaagg atagcgcgtc accagatcgt tgccgggcag gtgcggctcg ccctggaaat
      421 tgagcgcatt ggcaaagcgg ttggcgcagg tttcaaggcg gcggtcgcag ccttcgcgca
      481 gcatcaggcg gcagccgggg gacggggcct gatcgagcgg caggtcgagc acgagcgcgc
      541 cgccggttgc gcccaggatt acggcttcga gcccgcaata ggggccatcg agccagcgca
      601 gggttccgcc gaccacgttc gcgagcggga ccgtggtggc gacggtcgcc gcgttgctgg
      661 ccaggctgaa cccggtcagc gtggcttcgt gggtaaagcg cgcgttcgaa aggccgcagc
      721 cagggccgca gaagctggcc cggcagcttg ggctggtgcg cgggatcgga tcgcgggaca
      781 gttccagctt gcgcgattgc agcgccgcgg tgaacgatcc ggcctgttcg tttaccgccc
      841 cgatcgtacc gcgatagatc acgtgctgtt cgaagctttc ccagtcgacc agcccgatca
      901 gcacgcgcgc gccgtcgaag cggccctgcg ccagatcgct cgccgaaatc gaatcgtggc
      961 tcagcgcgcc ctcgacctcg gcgctgtcgg gttcgaaatc ggccgagcgg cggattgccg
     1021 atggcaccat gcccggggtg gcaaggtgca ggacatgatc gaaccacaga tcgcgatcgt
     1081 gggtggtgaa gcccagcgtc accccgtcgc ggcggatcac ccgccagaag gtggcaaccg
     1141 tttcgagttc gccggaaaac cacacccggc tcatgccgaa gcctcgcgga tctcgatcac
     1201 cggcacgctg ggtgcttcgc ccgcggcgaa aaccgcgccg gcaacttcaa gcttgtcttc
     1261 ggcaaagcgc accggcacgt cgaacaggaa cccggcccgc accgccttgc ccggcgctgg
     1321 gggtgcggca aagcggatca cccccagcgg atcgagcgtc caggccgtgc tggccacgcc
     1381 atcgatgctg accacaatcg atcccgccac cggccgggtg atccgccgca attgttcggc
     1441 atcgccgctg ccatagcgct tgaccagcgc gaagctggcg cgggtgccat cgccggtgcc
     1501 gagcagctgg tcggcatggg tcggcgcacc ggtcatgccg ttagaactgc aatccgacgg
     1561 atcgcgcagc ctgaagccgc gcgccgcgcc gcgccgggcc cggaagaagg caatcaaggt
     1621 gcctagctcg gcctcggagc ggattcccgg accgacatcg aagcgcagcc gggcatcggc
     1681 ccacaggcta ttgcggcgtt caaagcccga tgcggtaacc gaaacgctgg tcgagaattc
     1741 ggggatcacc atggcatcgc ggccaagcgc cagcgggtag gacagatcgt cgaaggcaag
     1801 catggtatcc tcttcgcttg gggagggcag gcgcaggtat ccgtcgcggc agacctgggg
     1861 cagcgcccag acaaagcgtt cgtgcgcctg gcgctgcgcg gcttcgtcga gcccggcatc
     1921 gatccggcgc cacaggtgcc tgtccgccgg gttcagcacg aagccggcaa gatagtcctg
     1981 ctggaggggc ggatagccca gccgctggtc gatctcggca taggcgctgc ggcgataggc
     2041 ctctgcgccc tgagtcagcc aatcatagtc ctcgcactgc agccggtcga aggctggcgc
     2101 ggcccagccg gttggcagat tggcgcggcg cacctcgggc atcgccggat cgagcacgcc
     2161 ggggaggaaa gtgagcagca ggacctcggc cacctgcggc gcggcgacag cgcgcaccgc
     2221 atcgcgcagc gcagcggtgg aggcggcaag cagcgctccg gccgcatcga gcaaggcctt
     2281 ctgcgcggca ttcaacggct ggctgaggtc ggcaatctcg accggactgc ccccgaacgc
     2341 ggcctttgcc gcatcgtcgt agaggcaggg gcggccatcg gccatgatcc accaccacgg
     2401 ctcgccgatc tggaagcgca ccggcaccgc ggcatccttc atcagcccgg caaaggcact
     2461 gccgactgcc tggagccagg ccattgccgc gctgtttgca ggcgagagca gcgacgaggg
     2521 cggatcccac ccggtaagcg ccggatcgcc gttgaaggcg cgctgcttcc acgcatcggg
     2581 acagtgctgg gcaagcagct cgtaggata
//
LOCUS       DASUDV010000395         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_764251,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000395 DASUDV010000000
VERSION     DASUDV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764251"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="tmk"
                     /locus_tag="VFV30_08555"
     CDS             complement(<1..563)
                     /gene="tmk"
                     /locus_tag="VFV30_08555"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX4848182.1"
                     /translation="MTRGMFIAFEGGEGAGKSTQARLLAEALRQRGLTCAVTREPGGT
                     PGAEAIRHLLLHSEGEGWNPRAEALLFAAARSDHVERLIRPAVEAGTWVICDRFVDSS
                     RAYQGGGGGLSDAEVLDLHRIGSQGLLPDLTLLIEVSPAVARERAAARDVDGADRIGG
                     RDEAYHAKVAAAFARFAQAEPHRFARI"
     gene            complement(560..1693)
                     /locus_tag="VFV30_08560"
     CDS             complement(560..1693)
                     /locus_tag="VFV30_08560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX4848183.1"
                     /translation="MLAFAAASARALSPPAPTPSPPAADQVPVALLVDLSSGQTLFAR
                     DPDAPFLPASMTKAMTALVAFDLIAQGRLKENAIVTVRPETAARWAGKGTTLWLQPGE
                     QVAVSDLLHGIVTASANDAAVALAESALGSEAAWLSAMNARAKALGLTGSHFASANGL
                     PDGGQTKVTARDMVRLGEALITEHPALYRRYFGHKEMAWKGQRLASRNPLAGLVPGAD
                     GIKTGHTREAGYTFLGAAERDGRRLVLVVGKVPSEAGRAAAARDLVEWGYGAWDSRPL
                     LSPGWVVGAVKVQDGAAREVPVTVPRGFSLAVPKGTRPRIQGTIVYQGPVRAPVAKGA
                     PIATLEIMIEGQPVYRLPLIAAQPVSKAGPIDRIINALLGLTQ"
     gene            complement(1770..>2119)
                     /locus_tag="VFV30_08565"
     CDS             complement(1770..>2119)
                     /locus_tag="VFV30_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4848184.1"
                     /translation="AIVTVRPETAARWAGKGKGKPAPATPAATAAKPAPAPAPKPAAA
                     PAKGQFFVQVGAFSSKANADAAAKKAGGSVVQAGNVWRVRTGPFATETDARAALAKAK
                     SAGYSDARIQRAD"
BASE COUNT          285 a          750 c          769 g          315 t
ORIGIN      
        1 tcgatccggg cgaaacggtg cggctcggct tgggcaaagc gggcaaaggc ggcggcgacc
       61 ttggcgtgat aggcttcgtc ccggccgccg atccggtcgg ccccgtccac atcgcgcgcg
      121 gcggcgcgtt cgcgggcgac agcgggcgag acttcgatca gcagggtcag gtcgggcagc
      181 aggccctggc tgccgatccg gtgcaggtcc agcacttcgg catcggagag gccgccgccg
      241 ccgccctggt aggcccggct cgaatcgacg aaacggtcgc agatcaccca ggttccggcc
      301 tcgaccgcgg ggcggattaa gcgctcgaca tggtccgagc gggccgccgc gaagagcagc
      361 gcctcggccc ggggattcca gccctcgccc tcgctgtgga gcagcaggtg gcggattgcc
      421 tcggcccccg gggtgccgcc gggctcgcgc gtgacggcgc aggtcagccc gcgctggcgc
      481 agggcttcgg ccagcagccg ggcctgggtg cttttcccgg ccccttcgcc gccttcgaag
      541 gctatgaaca ttccgcgcgt cattgcgtca gccctagcag ggcattgatg atcctgtcga
      601 ttggcccggc cttcgaaacc ggctgtgcgg cgatcagcgg caagcggtag accggttggc
      661 cttcgatcat gatctccagc gtggcgatcg gcgcgccctt ggcgaccggc gcacggaccg
      721 gcccctgata gacaatcgtg ccctggatcc gggggcgggt gcccttgggc acggccaggc
      781 tgaagccgcg cgggaccgtg accggcactt cgcgggccgc gccgtcctgc accttgaccg
      841 cgccgaccac ccagccgggg ctcaggagcg ggcggctgtc ccacgcgcca tagccccatt
      901 cgaccagatc gcgcgccgcc gcggcccggc cggcctcgct tggcaccttg ccgaccacca
      961 gcacgagccg ccggccatcc cgctccgccg cgccgaggaa ggtatagcca gcttcccgcg
     1021 tgtggccggt cttgatcccg tccgcgccgg gaaccagccc ggccagcgga ttgcggcttg
     1081 ccaggcgctg gcctttccag gccatctcct tgtgcccgaa gtagcggcgg tagagcgcgg
     1141 ggtgctcggt gatcagcgcc tcgcccagcc gcaccatgtc gcgcgcggtc accttggtct
     1201 ggccgccatc gggcaggccg ttggcactgg caaaatggct gccggtcagc cccagggcct
     1261 tggcccgcgc gttcatcgcg gacagccagg ccgcttcgct gcccagcgcg ctttcggcca
     1321 gggccacggc ggcatcgttg gcgctggcgg tgacaatccc gtggagcaga tcggacaccg
     1381 ccacttgctc accgggttgc agccataggg tggtgccctt cccggcccag cgcgcggcgg
     1441 tttcgggccg cacggtgacg atggcgtttt ccttgagccg cccttgggcg atcagatcga
     1501 aagcgaccag cgcggtcatc gccttggtca tcgaggccgg caggaacggc gcatcgggat
     1561 cgcgcgcgaa cagggtctgg ccggacgaca ggtcgaccag cagggccacc ggcacctggt
     1621 ccgcagcggg gggcgaagga gtgggagccg gcgggctgag cgcgcgcgcc gatgcggctg
     1681 caaacgccag caccagcaca ccatgcgata gcttcaaggc ctgtccccgt gtccggccag
     1741 ccggggccgg agacacggag gaagaccggt cagtcggcgc gctggattcg tgcgtcgcta
     1801 tagcccgcgc tcttggcttt cgccaaggcc gcccgggcgt cggtttcggt ggcgaaaggc
     1861 ccggtccgga cgcgccagac attgccagcc tggaccacgc tgcccccagc cttcttggcc
     1921 gcggcatcgg cgttggcctt gctggagaac gccccgacct ggacgaagaa ctggcctttg
     1981 gccggagcgg ccgccggctt gggtgcgggt gcgggggcag gctttgccgc ggtcgcggcc
     2041 ggagtggccg gagcaggctt gcccttgccc ttcccggccc aacgcgcggc ggtttcgggc
     2101 cgcacggtga cgatggcgt
//
LOCUS       DASUDV010000396         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_631748,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000396 DASUDV010000000
VERSION     DASUDV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631748"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFV30_08570"
     CDS             <1..198
                     /locus_tag="VFV30_08570"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEX4848185.1"
                     /translation="TGWPSFWKPLPGALATSTDWETGYPRTEVHCTRCGGHLGHVFDD
                     GPKPTGKRYCINGDALLFRPA"
     gene            complement(156..1925)
                     /locus_tag="VFV30_08575"
     CDS             complement(156..1925)
                     /locus_tag="VFV30_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848186.1"
                     /translation="MAWLVVSVLLVLRQLAAIRGFELVDPDDALRLVQVRDLIAGQGW
                     FELFQHRIGPAGGTATHWSRLVDLPLAATIVLLAPLVGQANAELAALIAVPLATLLCA
                     MALAARLAAKLLGEKAGWFSCLVWVLALPAVMQLQPMRIDHHGWQIVAALGAANALLA
                     RNPHTGGWIAGLSLAAGLSISLELLPFTVLVAAVLGLRWLRDAQARDWLQHMMLALAI
                     GSVGFYLGTRGLNAVENHCDTISPAYLAGFGFAAALVGAIAVLPQVSRPMLVVLLGAA
                     GALTGALYLQLAPQCRSGPFAALDPLVYQVWYVNVLEGMPVWKQQVPNLLQMLVPPLA
                     ALAILIFSLRHARDARRQGLLDLALLVTGSILVSLFVARFSTVSAALAVVPLGYGLAE
                     WLRRSASLSLAWRLCAVPVIALILLPSAIVRPLLDQADAPARRPIAPVFAAAGKGCSL
                     PAALPALRGIAPSTLFAPFEFGPSVLLHTRHRVIATNHHRASAGMAAVIRGFMADPAA
                     AEPFVRATGAHYVLACADLVEAGQYRERAPAGLMAHLLAGKPVAWLEPVALGPQAGTL
                     RLWKVLPPGDQAGRNSKASPLMQ"
BASE COUNT          400 a          748 c          728 g          259 t
ORIGIN      
        1 accggctggc ccagtttctg gaagccgctg cccggcgcgc ttgccacttc gaccgattgg
       61 gaaaccggct atccgcgcac cgaggtgcac tgcacccgct gcggcgggca tctgggccat
      121 gtgttcgacg atggtcccaa acccaccggc aagcgttact gcatcaacgg cgatgccttg
      181 ctgttccgcc ccgcctgatc gccaggcggc agcactttcc agagcctgag cgttccggcc
      241 tgcggcccca gcgccacggg ttccagccag gcaaccggct ttccggcaag caagtgcgcc
      301 atcagccccg ccggcgcgcg ttcgcggtat tgcccggctt cgaccagatc ggcgcaggcc
      361 aggacatagt gcgcgcccgt ggcccgcacg aacggttcgg ccgcggcggg atcggccatg
      421 aagccgcgga tcaccgccgc catcccggcc gaggcgcggt ggtggttggt ggcgatcacc
      481 cggtgccggg tatggagcag aacgcttggc ccgaactcga agggtgcgaa cagcgtgctc
      541 ggcgcaatgc cgcgcagcgc ggggagcgcg gcaggcaggc tgcacccctt gcccgcggcc
      601 gcgaagaccg gcgcgatcgg gcgccgtgcc ggggcatcgg cctggtccag cagcggccgc
      661 acgattgcgc tgggcagcag gatcagcgcg atcacgggta ccgcgcagag ccgccaggcc
      721 agcgacagcg aggccgagcg gcgcagccat tcggccaggc catagcccag cggcaccacc
      781 gccagggccg ccgaaaccgt actgaaacgc gccacgaaca ggctgacgag gatcgacccg
      841 gtcaccaaaa gggcaaggtc aagcaatccc tggcggcgcg catcgcgggc gtgacgcagg
      901 ctgaagatca ggatggccag ggcggccagc ggcgggacca gcatctgcag caggttgggc
      961 acttgctgct tccacaccgg catgccttca agcacgttga cgtaccagac ctggtagacc
     1021 agcggatcga gcgcggcgaa gggcccgctg cggcactgcg gcgcgagctg gaggtaaagt
     1081 gcgccggtca gcgcgccggc agcgcccagc aggaccacca gcatcgggcg cgaaacctgc
     1141 ggcaaaacgg caatcgctcc aaccagcgct gcggcaaatc cgaaaccggc gaggtaggct
     1201 ggcgaaatcg tatcgcagtg gttctccacc gcattgagcc cgcgtgtgcc aagatagaat
     1261 ccgaccgagc caatggccag ggccagcatc atgtgctgca gccagtcgcg agcctgagcg
     1321 tcgcgcagcc agcgcaggcc cagcaccgcc gcaaccagca cggtgaaagg cagcagttca
     1381 aggctgatcg acaggccggc cgccaggctc agccctgcga tccagccgcc ggtgtgcggg
     1441 ttgcgcgcca gcagcgcgtt ggcggccccc agagcggcaa cgatctgcca gccgtggtga
     1501 tcgatccgca tcggctgcaa ctgcatcacc gccggcaggg ccagaaccca gaccaggcag
     1561 gagaaccagc cggccttctc gcccagcagc ttcgcggcca gccgcgcggc cagcgccatg
     1621 gcgcacagca gcgtggcgag gggcacggcg atcagcgcgg cgagttcggc attggcctgt
     1681 ccgaccagcg gcgcaagcag cacgatcgtg gccgcgagcg gcagatcgac cagccgcgac
     1741 cagtgcgttg cggtaccgcc cgccggtccg atccggtgct ggaacagttc gaaccagcct
     1801 tgtccggcaa tcagatcgcg gacctggacc agtcgcagcg catcgtcggg atcgaccagt
     1861 tcgaagcccc ggatcgcggc cagctggcgc agcaccagca gcaccgaaac caccagccag
     1921 gccaacagta ccttaggcca gaagccatag ggaagaggca ggaggccgcc ggcctcgcgg
     1981 ttcatgttgc ggggcccgcg cggaagatca cgcgctcgcg catcagccag gtaagcgtaa
     2041 agctggcagc gatggcgatc agtttcgcca ggcgcgggtc aagccccatc atcccgccca
     2101 gcccgacaat cgcggtggtc agcccaaggc ccagc
//
LOCUS       DASUDV010000397         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1825032,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000397 DASUDV010000000
VERSION     DASUDV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825032"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFV30_08580"
     CDS             <1..755
                     /locus_tag="VFV30_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX4848187.1"
                     /translation="IDAGKGRDDIVTPGHVFPLIARDGGTLVRAGHTEASVDISRLAG
                     LNPSGVICEIMNEDGTMARMDDLIPFARRHGLKMGTIRDLIEYRRKNDRLVECVSEAP
                     FVSDYGGEWTAKTYRNKVDGSVNIVLQKGHVVPGEPTLVRMHSISIFSDVLGKPGPRK
                     RTLQRAMDAIGANEAGVIVLLMPNSPDQLSAEVGGVKGPEMDLRAYGIGAQILADLGI
                     HDMILLTNSHRNIVAIEGYGLNIVGERPIQEG"
     gene            760..1179
                     /gene="ribH"
                     /locus_tag="VFV30_08585"
     CDS             760..1179
                     /gene="ribH"
                     /locus_tag="VFV30_08585"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX4848188.1"
                     /translation="MARFLIVEARFYDHLNDMLVDGARAALNAAGHQVDVITVPGALE
                     IPGAIVLADQAQLYDGFVAIGVVIRGETYHFEIVAGESARGIMALTMDGMAIGNGILT
                     VENEAQALVRADPKQKDKGGEAAKAALALLALKEKFG"
     gene            1176..2099
                     /locus_tag="VFV30_08590"
     CDS             1176..2099
                     /locus_tag="VFV30_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4848189.1"
                     /translation="MTQHPAIAGIPLVLGGNVFGFTANEDASFAILDAFYEAGGRMID
                     TADVYSAWVPGHKGGESETVLGKWFAASGKRTEMRLHTKTGMLGGQELYEPARILTSL
                     DASLERLQTDYVDLYYAHRDYEELAMADIVAAFNGAAATGKVRSLGASNFSAERLTAA
                     LDAAEQQGAMPFSVLQNEYNLVARQAYGADLQQLCTARGIPMLPFFGLASGFLTGKYR
                     TADDLGKSVRGARMTTLIETGQPMLAAMDAVVRETGATHAQVALAWLRVQPGIGAPIA
                     SATSVEQLRELCGAASLTLSEDQLARLSAAL"
     gene            2110..2940
                     /locus_tag="VFV30_08595"
     CDS             2110..2940
                     /locus_tag="VFV30_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4848190.1"
                     /translation="MIDPAALLMLASGAAHAVVNAVLKAGGDKMSSRALIDGSSALLI
                     LPLAFVVPLPHGAWPWLFASFAVHLVYLVCVIKAFEGADMSAVYPVMRGTAPVLAAVA
                     SVLWLGDPISPGVALGVGLVSAGTALVAWWNAPSKRALAWALATGATIAAYTVIDAKG
                     ARAAPSAISYIVWVFLMLGFGIGAIFAAWRGPRFLAAARSEWKPGLIAGALSLVTYGL
                     ALWAYRIGDVPRLAALRESSILFGVLIAWLVLKERVGAPRLAGAGLIAGGAALLLLAR
                     "
BASE COUNT          465 a         1013 c         1034 g          485 t
ORIGIN      
        1 cgatcgacgc cggcaagggc cgcgacgaca tcgtcacccc cggccacgtc ttcccactga
       61 tcgcccgcga cggcggcact ctggtccgcg ccggccacac cgaagcttcg gtcgatattt
      121 cgcggctggc cgggctcaac ccttcgggcg tgatctgcga gatcatgaac gaggacggga
      181 ccatggcgcg gatggacgac cttatcccct tcgcccgccg ccacggcctg aagatgggca
      241 cgatccgcga cctgatcgaa taccgccgca agaacgaccg gctggtcgaa tgcgtcagcg
      301 aggcgccctt cgtctccgac tatggcgggg aatggaccgc caagacctat cgcaacaagg
      361 tcgacgggtc ggtcaacatc gtcctgcaaa agggccatgt cgtgccgggc gaaccgaccc
      421 tggtgcggat gcacagcatt tcgatcttct ccgacgtgct gggcaagccc ggcccgcgca
      481 agcgcacgct gcagcgcgcg atggacgcca tcggcgcgaa cgaggccggg gtgatcgtgc
      541 tgctgatgcc gaattcgccc gatcagctca gcgccgaagt cggcggggtc aaggggccgg
      601 aaatggacct tcgggcctat ggcattggcg cgcagatcct ggccgatctg gggatccatg
      661 atatgatcct gctgacgaat tcgcaccgca acatcgtggc catcgaaggc tacggcctca
      721 acatcgtggg cgaacgcccg atccaggaag gataagccca tggcccggtt tctgattgtc
      781 gaagcccgct tctacgacca tctcaacgac atgctggtcg atggcgcgcg cgccgcgctc
      841 aatgcggcgg ggcatcaggt cgatgtcatc accgtccccg gcgcgctgga aattcccggc
      901 gcgatcgtgc tggccgacca ggcccagctc tatgacggct tcgtcgcgat cggcgtggtg
      961 atccgcggcg agacctatca cttcgagatc gtcgccggcg aaagcgcgcg cgggatcatg
     1021 gcgctgacca tggacggcat ggcgatcggc aacggcatcc tgacggtcga gaacgaagcg
     1081 caagccctcg tccgcgccga tccgaagcag aaggacaagg gcggtgaagc cgccaaggcc
     1141 gccctcgccc tcctcgcgct caaggagaag ttcggttgac ccagcaccct gccatcgccg
     1201 gcatcccgct tgtcctgggc ggcaacgtct tcggctttac cgccaacgag gacgccagct
     1261 tcgccatcct tgacgccttc tacgaggccg gcgggcggat gatcgacacg gccgatgtct
     1321 attcggcctg ggtcccgggc cacaagggcg gcgagagcga gaccgtgctg ggcaagtggt
     1381 tcgccgccag cggcaagcgc accgaaatgc gcctccacac caagaccggc atgctgggcg
     1441 gccaggagct gtacgaaccg gcccgcatcc tgacctcgct cgatgcctcg ctggagcggc
     1501 tgcagaccga ctatgtcgat ctctattacg cccaccgcga ttacgaagag ctggcgatgg
     1561 ctgacatcgt ggccgccttt aacggcgccg cggcgaccgg caaggtgcgt tcgctcgggg
     1621 cctcgaactt ttcggccgag cgcctgaccg ccgcgctcga tgcggccgag cagcagggcg
     1681 cgatgccctt cagcgtgctg cagaacgaat acaatctggt cgcgcgccag gcctatggcg
     1741 cggacctgca gcagctctgc accgcgcgcg gcattccgat gctgccgttc tttggcctcg
     1801 cctcgggctt cctcaccggc aagtaccgga cggcggatga cctcggcaag tcggtgcgcg
     1861 gggcgcggat gaccacgctg atcgaaaccg gccagccgat gctggcggca atggacgcgg
     1921 tggtccgcga aaccggcgcg acccacgccc aggtcgcact ggcctggctg cgggtccagc
     1981 ccgggatcgg cgcgccgatt gccagtgcga cttcggtcga gcagctgcgc gaactgtgcg
     2041 gggccgccag cctgaccctc agcgaagacc agctcgcccg gctttccgcc gcgctgtgat
     2101 ccaggccgcg tgatcgatcc cgccgcgctg ctgatgctgg catcgggcgc ggcccatgcc
     2161 gtcgtcaacg cggtgctgaa ggcgggcggc gacaagatgt cgagccgggc gctgatcgac
     2221 ggatcgagcg cgctgctgat cctgccgctg gcatttgtgg tgccgctgcc gcacggtgcc
     2281 tggccgtggc tgttcgcctc tttcgcggtc cacctggtgt acctggtctg cgtgatcaag
     2341 gccttcgaag gcgccgacat gagcgcggtc tatccggtga tgcgcgggac cgcgccggtc
     2401 ctggcggcgg tggcatcggt gctgtggctg ggcgatccga tcagcccggg cgtggcgctg
     2461 ggcgtgggcc tggtcagcgc cggaaccgcc ctggtcgcct ggtggaacgc gccgagcaag
     2521 cgggcgctgg cctgggcgtt ggcgaccggg gccaccatcg ccgcctacac ggtgatcgat
     2581 gccaagggcg cccgcgccgc gccctcggcc atcagctaca tcgtctgggt gttcctgatg
     2641 ctgggcttcg ggatcggggc gatctttgcc gcctggcgcg gcccacgctt tctggccgcg
     2701 gcgcgcagcg agtggaaacc cgggctgatc gccggggcac tctcgctcgt cacctatggc
     2761 ctggcgctgt gggcctaccg gatcggcgac gtgccgcggc tggcggcgct gcgcgaaagc
     2821 tcgatcctgt tcggtgtgct gatcgcctgg ctggtgctca aggaacgggt cggcgcgccg
     2881 cggctggccg gcgccgggct gatcgccggc ggcgcggccc tgctgctgct ggcgcggtga
     2941 tcagcccttg atcggagcgc aggagtccgc gcggcgggcc tgatcgacaa tggcgaa
//
LOCUS       DASUDV010000398         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1957180,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000398 DASUDV010000000
VERSION     DASUDV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957180"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFV30_08600"
     CDS             complement(<1..506)
                     /locus_tag="VFV30_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848191.1"
                     /translation="MTRSLFAAATALFALALPSSASAATCEETFVKKGNGITGLRFIA
                     MTSVPDMPVDVAINQLRGIVARRGYDIIAVEPAAGAMLIEQSQTGNSRAFPIEINATT
                     DNGAGTVQMEAKLRAGQFTKAELAKAEMCAVLAELKGGKAGKLAAAAGARAVTQQAAP
                     IAMSAQAFS"
     gene            663..1418
                     /locus_tag="VFV30_08605"
     CDS             663..1418
                     /locus_tag="VFV30_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4848192.1"
                     /translation="MQLLADPLTLALALVAVALLGLAKGGFAGLGALATPLLALTLPP
                     VTAAALLLPVLLVQDVVSVWSYRHSWERWIVCWMLPGAALGVGLATLMAALIPESTFL
                     LVLGLLTLAFGFYRLWIERGGRIVTASSSPGWVGSLFGVGLGFASQIAHAGGPPFQMW
                     VAPRRLPQVVYSGTGSVLFAAVNWIKVPSYMALGSFTPDILVAAALLAPLAVLATLAG
                     VWLVRRIDTARFYTITYSLMILLGLRLIWQGLV"
     gene            complement(1415..1918)
                     /locus_tag="VFV30_08610"
     CDS             complement(1415..1918)
                     /locus_tag="VFV30_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848193.1"
                     /translation="MKRLTIVALSFAMLAGCVADKTPQQARTPAPAARQAALPAPVPQ
                     PIKPAPTAPASHAVADPGEAVWHLRSGLNVAALSCRGKGRAPVSGDYARLLTRHKALL
                     ASAYAGEQRRYGKGLDRHLTQLYNRFAFQRSPERFCASAAAVAAEAVRIDSAALSARA
                     RNLLERI"
BASE COUNT          292 a          656 c          705 g          377 t
ORIGIN      
        1 gagaaggcct gggcgctcat cgcgatcggg gcggcctgct gagtgaccgc cctggccccg
       61 gctgccgccg ccagcttgcc cgccttgcca cccttcaatt cggccagcac cgcgcacatc
      121 tcggccttgg ccagttccgc cttggtgaac tgtccggcac gcagcttggc ctccatctgg
      181 accgtgccgg caccgttgtc ggtggtggca ttgatttcga tcgggaaggc ccggctgttt
      241 ccggtctgtg attgttcgat cagcatcgcc cccgccgcgg gttcgaccgc gatgatatcg
      301 tagccgcgcc tggcgacgat accgcgcagc tggttgatcg cgacatcgac cggcatgtcg
      361 ggaacgctgg tcatcgcgat gaagcgcagg ccggtgatgc cgttgccctt cttgacgaag
      421 gtttcctcgc aggtggcggc cgaagcagag ctgggcagtg ccaacgcgaa cagggcggtt
      481 gcagcggcaa acagggatct ggtcatcatg ggtgtgcgga cctcgtgctg gatgggacag
      541 gacaatggct gaaggacatg acggagccgc gggcccattg ccttgcccgg ttatgccagg
      601 ccggggacaa gatcggcatc gacgcttgcg cagagccggg cgaagtgctg cgaaggttgg
      661 cgatgcaact gctggccgat ccgctgaccc ttgcgctcgc acttgtcgcc gttgccctcc
      721 tcgggctagc caagggcggc tttgcgggtc ttggcgcact ggccacgccg ctgctggcac
      781 tgaccctgcc cccagtcacc gccgccgcgc tgctgctgcc ggtgctgctg gtgcaggatg
      841 tcgtctcggt ctggtcctat cgtcacagct gggagcgctg gatcgtttgc tggatgctgc
      901 ccggggcagc gctgggggtg ggcctggcaa cgctgatggc cgcgctgatc ccggaaagca
      961 ccttcctgct ggtgctgggc ctgctgacgc tcgccttcgg attctatcgg ctgtggatcg
     1021 aacgcggcgg gcggattgtc actgcttcga gctcgccggg atgggtcggc agcctgttcg
     1081 gagtggggct gggattcgcc agccagatcg cccatgccgg cggccccccg ttccagatgt
     1141 gggttgcacc gcgccgcttg ccgcaagtgg tctattccgg caccggatcg gtgctgttcg
     1201 ctgcggtcaa ctggatcaag gttcccagct acatggcgct gggttcgttc acgcccgata
     1261 tcctggtggc cgcagccctg ctggcgccac tggcggtgct ggcaaccctg gccggggtct
     1321 ggctggtgcg ccggatcgat accgcccgct tctacacgat cacctatagc ctgatgatcc
     1381 tgctgggcct caggctgatc tggcagggtc tggtctagat ccgctcgagc aggttgcggg
     1441 cccgggcgga aagcgcagcc gaatcgatcc gcactgcttc agccgccacg gccgccgcgc
     1501 tggcgcagaa ccgttcgggc gaacgctgaa aggcaaagcg gttgtagagc tgggtaagat
     1561 gacgatcgag ccccttgcca tagcggcgct gctcgccggc ataggccgat gccagcagtg
     1621 ccttgtggcg ggtcagcagc cgcgcatagt cacccgagac cggggcacgt cccttgcccc
     1681 ggcacgacag cgcggcaacg ttcagcccgg agcgcaggtg ccagaccgct tcccccggat
     1741 cggccacggc atggctggcc ggagcggtcg gcgcgggctt gatcggctgc gggaccggtg
     1801 cgggcaatgc agcctgacgc gccgccggtg ccggtgtgcg cgcctgctgc ggcgtcttgt
     1861 cggcaacgca gccagccaac atggcaaacg aaagggcaac gattgtcagg cgcttcatcc
     1921 aggcgacctc cttgtccggc gcatggcagg cccgtcggct tgccaaaggc atgcggcaat
     1981 atcgctgcgc ccggttgccc gctgcttgcg gcaaatgctg aatcgggtgt
//
LOCUS       DASUDV010000399         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_720332,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000399 DASUDV010000000
VERSION     DASUDV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720332"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFV30_08615"
     CDS             <1..297
                     /locus_tag="VFV30_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848194.1"
                     /translation="DFFNRLERDGAEKTIASLFPSVTANDPAMAAMKELDRDCGAVTK
                     VDRYKTVTFGSRAVRDEFVVLMGSCLVKWELTYLKTGVAWKLNNVHFNSSPGDW"
     gene            355..1104
                     /locus_tag="VFV30_08620"
     CDS             355..1104
                     /locus_tag="VFV30_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4848195.1"
                     /translation="MKSLRFALAPLALSLALPGAAMAHDTQLTAIAPTSTLLTLSAEG
                     KSTRTPDIAVFSAGVVSEGKTASEALAANSAAMNKVIATLKKAGIADKDIQTSNISLN
                     PVYGQPVIGPNGQMIQEPRIIGYQANNTVTVRSRDIKGFGKVLDALVSSGANQVNGPN
                     FQMSDPSAANDEARANAMKAARARADLYAKAAGLRVVRIISISEGGGYAAPQPVYAKM
                     AMADASSAPTPISAGEMEMQVSVTVQFELAP"
     gene            1130..1774
                     /locus_tag="VFV30_08625"
     CDS             1130..1774
                     /locus_tag="VFV30_08625"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX4848196.1"
                     /translation="MSARPLIKICGITTPAALEAAIAGRASHTGFNFYAPSPRYAAPA
                     LAADLARQGEGRIARVGVFVDADDALLSEVLAAARLDALQFHGQESPQRVAQARRQFG
                     LPVWKVLSVASRDDLAKAAAYAEAADFLLLDAKTPKGTLPGGMGLSFDWALLNGWKAP
                     LPWGLAGGLSPRNVADAIRQTGAPLVDAASGIESAPGVKDPALIAAFCAAAHGA"
     gene            complement(1842..2303)
                     /locus_tag="VFV30_08630"
     CDS             complement(1842..2303)
                     /locus_tag="VFV30_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX4848197.1"
                     /translation="MRKFILPLLASLAVATPALANETRVEARGGVIWSDGDSEDTWGA
                     AIGYDFDLGANTFAGAEVSADKIGATGTKTAFGVTGRLGVKAGTGTRLFGAGGYTSEP
                     CDACEGQWHLGAGLEQNVSGPVYLKAEYRHYFENDVILGGDAVVAGVGIRF"
     gene            2538..>3206
                     /locus_tag="VFV30_08635"
     CDS             2538..>3206
                     /locus_tag="VFV30_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4848198.1"
                     /translation="MTAPAQKNSFKNQPDDRGHFGQFGGRYVAETLMPLILDLEREYT
                     AAKADPAFHAQFDDLLEHYVGRPSPLYFAPRLTEELGGAQIWFKRDELNHTGAHKINN
                     CIGQILLAIRMGKTRIIAETGAGQHGVATATVCARFGLPCVVFMGATDVARQAPNVFR
                     MKLLGAEVRPVTAGAATLKDAMNEALRDWVANVHDTFYIIGTAAGPHPYPELVRDFQS
                     VIGKE"
BASE COUNT          558 a         1110 c         1040 g          498 t
ORIGIN      
        1 gatttcttca accgacttga gcgggacggc gccgagaaga ccatcgcgag cctgttcccc
       61 tcggtcaccg ccaacgatcc ggcgatggcg gcgatgaagg aactcgaccg cgattgcggg
      121 gccgtgacca aggttgatcg ctacaagacg gtcacatttg gctcacgggc cgtacgcgac
      181 gaattcgtcg tgctgatggg cagctgcttg gtgaagtggg aactgaccta cctgaagacc
      241 ggcgtggcct ggaagctcaa caacgtgcat ttcaattcca gcccgggcga ttggtagtca
      301 ttcagcatcg cgaaagcgcg gtgcgggtta aaccggcgac gaaggagtag acctatgaaa
      361 tccctgcgct ttgccctcgc cccgctcgcc ctctcgcttg ccctgcccgg agctgccatg
      421 gcccacgaca cccagctgac cgcaatcgcg ccgaccagca cgctgctgac cctttcggcc
      481 gagggcaagt cgacccgcac gcccgacatc gcggtgttca gcgccggggt ggtcagcgaa
      541 ggcaagaccg ccagcgaggc gctcgccgcc aacagcgcgg cgatgaacaa ggtgatcgcc
      601 acgctgaaga aggcgggaat cgccgacaag gacatccaga ccagcaacat cagcctcaac
      661 ccggtgtacg ggcagccggt gatcggcccc aacggccaga tgatccagga accgcggatc
      721 atcggttatc aggccaacaa caccgtcacc gtccgcagcc gcgacatcaa gggcttcggc
      781 aaggtgcttg acgcgctggt ctcgtccggc gcgaaccagg tcaacggccc gaacttccag
      841 atgtccgatc cctcggccgc caacgacgaa gcccgcgcca atgcgatgaa ggccgcccgc
      901 gcccgggccg atctctacgc caaggctgcc gggctgcggg tcgtgcggat catctcgatc
      961 agcgaaggcg gcggctatgc cgcgccgcag ccggtctatg ccaagatggc catggccgac
     1021 gcgtccagcg cgccgacgcc gatctcggcc ggggaaatgg aaatgcaggt cagcgtgacc
     1081 gtgcagttcg aactggcgcc gtaagcccgg gcgcgctagg cggcgggtca tgtcggcccg
     1141 ccccctgatc aagatctgcg gcatcaccac gcccgctgcg ctggaggcgg ccattgccgg
     1201 ccgggccagc cacacgggct tcaatttcta tgccccctcg ccgcgctatg ccgccccggc
     1261 cctggccgcc gatctggcgc ggcagggcga agggcggatc gcgcgggtcg gcgtgttcgt
     1321 cgatgccgac gatgccttgc tcagcgaggt gctggccgcc gcgcggctcg atgccttgca
     1381 gtttcacggc caggaaagcc cgcagcgcgt ggcccaggcg cgccggcagt tcggcctgcc
     1441 ggtctggaag gttctgtcgg tcgccagccg cgacgatctg gccaaggccg cggcctatgc
     1501 cgaggcggcc gatttcctgc tgctggatgc caagacaccc aagggcaccc tgcccggcgg
     1561 gatggggctc agctttgact gggccctgct gaacggctgg aaggccccgc tgccctgggg
     1621 gctggcgggc gggctctcgc cgcgcaacgt cgccgatgcg atccgccaga ccggcgctcc
     1681 gctggtcgat gccgcatcgg gcatcgaatc cgctcccggc gtgaaggacc cggcgctgat
     1741 cgccgccttc tgcgccgccg cgcacggcgc ctgagcccca acggaaaagg gcggcccgtt
     1801 gccggaccgc ccttcccact cggtgttttc cgatcgagcg atcagaaacg gatgccgacg
     1861 ccggccacga cggcatcgcc gcccaggatg acgtcgtttt cgaagtagtg gcggtattcg
     1921 gccttcaggt agaccgggcc cgagacgttc tgctcaaggc cggcacccag gtgccactgg
     1981 ccttcgcagg cgtcgcacgg ctcgctggtg taaccgccgg cgccgaacag gcgggtgccg
     2041 gtgccggcct tcacgccgag acggccggtc acgccgaagg cggtcttggt gccggtcgcg
     2101 ccgatcttgt cggccgagac ttccgcgccg gcgaaggtgt tggcgcccag gtcgaagtca
     2161 tagccgatgg ccgcgcccca ggtgtcttcg ctgtcaccgt cggaccagat cacgccgccg
     2221 cgcgcttcga cgcgggtttc gttggccagg gccggggtcg caacagccag cgaggccagc
     2281 agcgggagga tgaacttgcg catgatatta ctcctaattc gcacgccccg tcgcatgggg
     2341 tcggaccccg aactaggaac cgccaccttg cattcccatg aaccacgcgt tcagaaattg
     2401 cgcgtgtatg attttatgtg accgttcggc aacactttgg gcggccagcg aatcattgtc
     2461 ttaccggccg cttcaccatt ggacgcagcg cgcaggctgg acttcccggg cccaccctgc
     2521 caaacgctga ccccgtcatg accgcacccg cacaaaaaaa ttcgttcaag aaccagcccg
     2581 acgatcgcgg gcatttcggc cagttcggtg ggcgctatgt cgccgaaacg ctgatgccgc
     2641 tgattctcga cctggaacgg gaatacaccg cggccaaggc cgatccggcc ttccacgccc
     2701 agtttgacga tctgctggag cattacgtcg gccggcccag cccgctctat ttcgcgccgc
     2761 gcctgaccga agagctgggc ggcgcgcaga tctggttcaa gcgggacgag ctgaaccaca
     2821 ccggcgcgca caagatcaac aactgcatcg gccagatcct gctggcgatc cgcatgggca
     2881 agacccggat catcgccgaa accggcgcgg gccagcacgg cgtcgccacg gccacggtct
     2941 gcgcgcgctt cggcctgccc tgcgtggtct tcatgggggc gaccgacgtg gcccgccagg
     3001 cgcccaacgt gttccggatg aagctgctcg gcgccgaagt gcgccccgtc accgccggag
     3061 cggcgacgct gaaggacgcg atgaacgagg cgctgcgcga ctgggtcgcc aacgtccacg
     3121 acaccttcta catcatcggc accgccgccg ggccgcaccc ctatccggaa ctggtccgcg
     3181 atttccagag cgtgatcggc aaggaa
//
LOCUS       DASUDV010000400         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1913061,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000400 DASUDV010000000
VERSION     DASUDV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913061"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFV30_08640"
     CDS             complement(<1..1059)
                     /locus_tag="VFV30_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4848199.1"
                     /translation="MTKELHLTLHGAARTVTGSCHEFTCAGSRVLVDCGLFQGSRTLE
                     GLNKGAFGFKPGQVDAVVLTHAHIDHSGLLPRLVAEGFTGPIWCTQPTADLLECMLAD
                     AGRIQEADAARHNRRRDRSRDEEFRPLYTEADALAAWQQCRTVELGEWFEPAPGFRAR
                     LWNAGHILGSASAELEAGGARVLCSGDLGPTNKAFHADPEGPAGFDHVLCESTYGDRE
                     REELSIAARRKLLEAEIRGALDRGGNLVIPTFALERTQELLLDIAELVRTGALPNVPV
                     FIDSPLASQATRVFARHAAELEDVDGACTFDCPNFHFTESVEQSIRLNSVSGAIIMAA
                     SGMCEAGRIRHHLIHNLFR"
     gene            complement(1079..2098)
                     /locus_tag="VFV30_08645"
     CDS             complement(1079..2098)
                     /locus_tag="VFV30_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4848200.1"
                     /translation="MSQASRLTARAAALTGITALCRSYRIDAARLLRQVGLPAGVEDQ
                     PDRRIPVTAVNAVFELAAKVTGRDDFGLRLAELRGFSNLGPIGLIARDEPTVGTAFAA
                     IEAYLPLHNDALSVTRQHFGDLVVLRSEIRTPGPMTQALDTAVAMQHRILRQLAGPAW
                     QAEEVCLTRAEPVDSARFRQVLGQRLTFNAEFDGIVVRAELLDRPNTMAEAAFRPYAS
                     HLLRTVSGGLADSMTARVQRVLALMLSSGRCTAGHVAQQLGVSRRTLTRALEAEGTRF
                     LALHDAARDEVARRHLAGQARSLSEISDLLGFSSPAAFSTWFRRRNAMSPRDWRLREV
                     KALSD"
     gene            2182..2565
                     /locus_tag="VFV30_08650"
     CDS             2182..2565
                     /locus_tag="VFV30_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX4848201.1"
                     /translation="MSTTPISDAARASGQWNKAWDQAAAFDAEWMEKFLDMGTHAARK
                     GIIDPKTYEFLAIAVDASCTHMYSPGVKRHIAKALDLGATPEEIMAVLQLVAVLGIHS
                     VALGTPHLVDEMKARGMPIPASKED"
     gene            2567..>3339
                     /locus_tag="VFV30_08655"
     CDS             2567..>3339
                     /locus_tag="VFV30_08655"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX4848202.1"
                     /translation="MHFLDDSLLPENQEKLVIQVAPYGPQWLPGDFPEDIPVTMADQV
                     QKAVDCYNAGATVLHLHCREADGSGSKRLSMFNEMIDRIRTACPDMLIQVGGSISFAP
                     EGEGGEAKWLSDDTRHMLAELKPKPDQVTIAVNTNQMDISALMSADDIAGTCLTNPAM
                     FEAYRNMYFECGPVFMEEHLKRLVANGIQPHFMVGDLSQLETVERLIRKGHYNGPLVL
                     NYVAIGSGAAGTHPADLIEFARRVPDGAVLTIESVMRNVA"
BASE COUNT          646 a         1078 c         1097 g          518 t
ORIGIN      
        1 ccggaacagg ttgtggatca ggtggtggcg gatccgccct gcctcgcaca tgcccgaggc
       61 ggccatgatg atcgcccccg acaccgaatt gagccggatc gattgctcga cgctttcggt
      121 gaaatggaaa ttgggacaat cgaaggtgca ggcgccgtcg acatcttcca gctctgccgc
      181 atgcctggcg aagacccggg tggcctgact ggccagcggg ctgtcgatga acaccggaac
      241 attgggcaag gccccggtgc gaaccagttc ggcaatatcg agcagcagtt cctgggtgcg
      301 ttcgagcgcg aaagtgggga tcaccaggtt gccgccacga tcgagcgcgc cgcgaatctc
      361 cgcctcgagc agcttgcgcc gcgcggcgat gctcagttcc tcgcgctcgc ggtcgccata
      421 ggtgctttcg cagagcacat gatcgaagcc ggcaggccct tcgggatcgg catgaaacgc
      481 cttgttggtc ggccccagat cgcccgagca cagcacccgc gctccgccgg cctcaagctc
      541 tgccgaggct gagccaagga tgtgcccggc gttccacagc cgcgcgcgga agcccggcgc
      601 gggctcgaac cattcgccca gctcgaccgt gcggcactgc tgccaggcgg caagggcatc
      661 ggcttcggtg tagagcgggc gaaattcctc gtcgcgcgag cggtcgcggc ggcggttgtg
      721 gcgcgccgca tcggcttcct ggatccgccc ggcgtcggcc agcatgcatt cgagcaggtc
      781 ggcggtgggc tgggtgcacc agatcggccc ggtgaagcct tcggcaacca ggcggggcaa
      841 cagcccggaa tggtcgatgt gggcatgggt cagcaccacc gcatcgacct ggccgggctt
      901 gaagccgaag gcccccttgt tgagtccctc tagtgtgcgc gaaccctgga acagtccgca
      961 gtcgaccagc acccggctgc ccgcgcaggt aaactcgtgg caggatccgg taaccgtgcg
     1021 cgctgcgccg tgaagggtaa ggtgaagttc cttggtcatg acctgctcct tcgcaggtct
     1081 agtctgacag cgctttgacc tcgcgcaagc gccaatcgcg cgggctcatc gcattgcggc
     1141 ggcgaaacca ggtggaaaag gcggccgggc tggaaaaacc cagcaggtcg gaaatttcgg
     1201 aaaggctgcg cgcctgcccg gccagatggc ggcgggcaac ctcgtcgcgg gccgcatcgt
     1261 ggagggccag gaagcgcgtc ccttcggcct cgagcgcgcg ggtcagggtg cggcgcgaca
     1321 cgccaagctg ctgggcaacg tggccagcgg tgcagcgccc gctggacaac atcagcgcca
     1381 gcacccgctg gacccgggcc gtcatgctat cggccaagcc gcccgagacc gtgcgcagca
     1441 ggtgcgaagc atagggtcgg aacgccgcct cggccatcgt attgggccgg tcgagcagct
     1501 cagcgcggac cacgatgcca tcgaattcgg cattaaacgt caggcgctgg cccagcacct
     1561 ggcgaaagcg cgcggaatcg accggttcgg cgcgggtcag gcacacttcc tcggcctgcc
     1621 aggcggggcc ggccagctga cgcaggatac ggtgctgcat cgccaccgca gtatcgagcg
     1681 cctgggtcat cgggccaggg gtgcgaattt ccgaacgcag caccaccaga tcgccaaaat
     1741 gctggcgggt cacgctcagc gcatcgttat gcagcggaag ataggcctcg atcgcggcaa
     1801 aggccgtgcc aaccgtgggc tcgtcgcggg caatcagccc gatcggaccc aggttcgaaa
     1861 agccccgcag ctccgccagc ctgagcccaa aatcgtcgcg tccggtaacc ttggcggcca
     1921 gttcgaacac ggcgttgacc gcagtgacgg gaatgcgccg gtcgggctgg tcctcgaccc
     1981 ccgcgggcaa gccgacctga cgcagcagcc gcgccgcatc gatccggtag gaccggcaca
     2041 gcgccgtaat tccggtcagc gcggcggcgc gtgcggtgag gcggctggct tggctcatat
     2101 tcgaaagaat atggcgcaaa atataaagca tgcaagcaaa gatcacgcca tgatggccaa
     2161 acgagaacaa aggggagagc gatgagcacg acgccgatca gcgatgccgc gcgcgccagc
     2221 ggccaatgga acaaggcctg ggatcaggcc gccgctttcg atgccgaatg gatggaaaag
     2281 ttcctcgaca tgggaaccca tgcggcccgc aaggggatca tcgatcccaa gacctacgaa
     2341 ttcctggcca tcgcggtcga tgccagctgc actcacatgt attcgccggg ggtcaagcgc
     2401 cacatcgcca aggcgctcga tctgggcgcg acacccgagg aaatcatggc cgtgctgcaa
     2461 ctggtggcgg tgctggggat ccattcggtc gcgctcggca cgccgcacct ggttgatgaa
     2521 atgaaagccc gcgggatgcc gattcccgct tccaaggagg actgagatgc atttccttga
     2581 cgatagcctg ctgcccgaaa accaggaaaa gctggtaatc caggtcgccc cttatggccc
     2641 gcagtggctg ccgggcgatt tccccgagga cattccggtg accatggccg accaggtcca
     2701 gaaggcggtc gattgctata acgccggggc caccgtgctg cacctgcact gccgcgaagc
     2761 cgatggcagc ggatcgaagc gcctgtcgat gttcaacgag atgatcgacc gcattcgcac
     2821 cgcctgcccc gacatgctga tccaggtcgg cgggtcgatc agctttgcac ccgaaggcga
     2881 aggcggcgag gccaagtggc tgtccgacga tacacgccac atgctggccg aactgaaacc
     2941 caagcccgac caggtgacga tcgcggtcaa caccaaccag atggatatct cggcgctgat
     3001 gagcgcggac gatatcgccg ggacctgcct gaccaacccg gcgatgttcg aagcctatcg
     3061 caacatgtat ttcgaatgcg gcccggtgtt catggaagaa cacctcaagc gcctcgtcgc
     3121 caatggcatc cagccgcact tcatggtagg cgatctcagc cagctggaaa cggtcgaacg
     3181 cctgatccgc aaggggcact acaatggccc gctggtgctt aactacgtcg ccatcggcag
     3241 cggtgcggcc ggcacccacc ccgccgacct gatcgaattc gcccgccgcg tgcccgatgg
     3301 cgccgtgctg acgatcgaat cggtgatgcg caacgttgc
//
LOCUS       DASUDV010000401         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1316751,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000401 DASUDV010000000
VERSION     DASUDV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316751"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFV30_08660"
     CDS             complement(<1..636)
                     /locus_tag="VFV30_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4848203.1"
                     /translation="MPNSKALFSIIAVADLAFPPREGGYTKGHETRELILRAALGLLI
                     EEGFRSVSMRRVAAASGMKLGNLTYHFPTREDLIQALFDAVISSYEIEFERIVHMPGV
                     APEKRLLAYCRLVLEDIRSKKTSHFFPEVWVLAIHDSFVADRMQELYARARRPLEEII
                     EEVRPDLPPAKRNDLALFISASMEGLTMFAGHERPFEPRMPIYEAIAARSFV"
     gene            806..1936
                     /locus_tag="VFV30_08665"
     CDS             806..1936
                     /locus_tag="VFV30_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HEX4848204.1"
                     /translation="MLAQPALANTTPPPLPDDPIPSVGKLPADYPKSWVLVHDFNFAS
                     LVDGRIAVVDTANPALPLKGFVRAAQFANMLHAPARHEIYTAETFYTRLTRGERTDAI
                     TIWDTATLQPKGEILLPGGKRQQSVTYTGTFQLTNGDKWALVSNFTPAQSITVVDLEG
                     RKVLGDIDFPGCSHIYPTGERGFTSFCADGSLISVQLDANGAVAASKVIEKVQDIDNQ
                     AQFQMPAMVGRTAWFVTYRGQIKGYDLSGPVAKPLPAKYTVGTADGAQGEWRPGGWNV
                     IAADAKGMLYVLMNPAGKEGSHKDGGSEVWVIDSASGQRTNRIVLQAHSGSIAVTRED
                     SPRLVVVRADTGIDVYDAASGAFVHSLGNRTAYTPTVIYPVP"
     gene            1936..2424
                     /locus_tag="VFV30_08670"
     CDS             1936..2424
                     /locus_tag="VFV30_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX4848205.1"
                     /translation="MQALVLFLAAILGSSALHKLLARDRLVPVVARLSGAGLPLATLL
                     LALAAMIEALAGLALLIGELRGGGLIAAILVWLGYGAVLARRRGEVLDCGCDLVSREK
                     PVDGLTIARPLFLALLAALLLAGPPPAWTLDAPFAALALLALWFAAAELAALPALKRK
                     PT"
     gene            2424..>2546
                     /locus_tag="VFV30_08675"
     CDS             2424..>2546
                     /locus_tag="VFV30_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase"
                     /protein_id="HEX4848206.1"
                     /translation="MMTSQVLLWVAVVILGVLVAALARQVGILHERIAPAGALTL"
BASE COUNT          420 a          831 c          826 g          469 t
ORIGIN      
        1 gacaaagctg cgcgcggcaa tagcctcgta gatcggcatg cgcggctcga acggccgctc
       61 gtgcccggca aacatcgtca gcccctccat cgaggccgag ataaacaggg ccagatcatt
      121 gcgcttggcc gggggcagat cagggcggac ttcctcgatg atttcttcca gcggccggcg
      181 cgcgcgggca tagagttcct gcatccggtc ggccacgaag ctgtcgtgga tcgccagaac
      241 ccagacttcg gggaagaagt ggctggtctt cttcgaacgg atgtcttcca gcaccagccg
      301 gcagtaggct agcagccgtt tttccggcgc gaccccgggc atgtgcacga tccgttcgaa
      361 ctcgatctcg taactgctga tcaccgcgtc gaacagcgcc tggatcagat cctcgcgggt
      421 cgggaagtga taggtcaggt tgcccagctt catcccgctg gccgccgcga ctcggcgcat
      481 ggagaccgag cggaagcctt cttcgatcag caggccaagg gccgcgcgca ggatcagttc
      541 gcgggtttca tggcccttgg tatagccgcc ttcacgcggc ggaaaggcca ggtccgccac
      601 cgctatgatg ctgaaaagcg ctttggaatt gggcatttga tcgacctcat cagcggcatt
      661 gaaaaccgga cattcgttaa aattaggaca cttgacaaag tttgtccagt gccgtcacta
      721 tccccctcat cgcggcaggc atcatcctgc gcgaaggagg ggaccgatgc gcatcagcca
      781 ccacgccttg gcgctttctt tgtttgtgct ggcacagccg gcactggcca acacgacgcc
      841 accgccactg cccgacgatc cgatccccag cgtgggcaag ctgccggccg actatcccaa
      901 gagctgggtg ctggtgcacg atttcaactt cgcctcgctg gtcgatggcc ggatcgcggt
      961 ggtcgatacc gccaatcccg cgcttccgct caagggcttt gtccgcgccg cgcagttcgc
     1021 caacatgctg cacgccccgg cccgccacga aatctacacc gcggaaacct tctatacgcg
     1081 gctcacccgt ggcgaacgga ccgacgcgat caccatctgg gataccgcca cgctgcagcc
     1141 caagggtgaa atcctgctgc ccggcggcaa gcgccagcaa tcggtcacct ataccggcac
     1201 cttccagctg accaatggcg acaagtgggc gctggtcagc aacttcacgc ctgcccaatc
     1261 gatcacggtg gtcgatcttg agggccgcaa ggtgctgggc gacatcgact ttccgggttg
     1321 ttcgcacatc tatcccactg gcgaacgcgg cttcaccagc ttctgcgccg atggttcgct
     1381 gatctcggtc cagctcgatg cgaacggggc ggtagccgcc tcgaaggtga tcgagaaggt
     1441 ccaggacatc gacaaccagg cccagttcca gatgccggcg atggtcggcc gcacggcctg
     1501 gttcgtgacc tatcgcggcc agatcaaggg ctatgacctg tcggggccgg tggcgaagcc
     1561 gctgcctgcc aagtacacgg tgggtaccgc cgatggcgca cagggcgaat ggcgtccggg
     1621 cggctggaac gtgatcgccg ccgatgccaa gggcatgctc tatgtgctga tgaacccggc
     1681 cggcaaggaa ggcagccaca aggatggcgg cagcgaagtg tgggtgatcg acagcgccag
     1741 cggccagcgg accaaccgca tcgttctgca ggcccattcg ggctcgatcg cggtcacgcg
     1801 tgaggatagc ccccgcctgg tggtggtccg cgccgatacc ggcatcgatg tctacgatgc
     1861 ggccagcggt gccttcgtcc actcgctggg caaccgcacc gcctatactc ccaccgtgat
     1921 ctatccggtg ccgtgatgca ggcgctggtc ctgttcctgg cggcgatcct tggctcatcc
     1981 gccctgcaca agctgctggc ccgcgatcgg ctggtgccgg tggtggcgcg gctgagcggc
     2041 gcgggtctgc cgttggcgac gctgctgctg gcgctggccg cgatgatcga ggcgctggcc
     2101 ggcctggcgc tgctgattgg cgaattgcgc ggcggcggcc tgattgccgc catcctggtc
     2161 tggctgggtt atggcgcggt actggcgcgg cggcgtggtg aagtgctcga ttgcgggtgc
     2221 gatctggttt cacgcgaaaa gccggttgat ggcctgacca tagcccgtcc gctcttcctg
     2281 gcgctgcttg ccgcgctgct gctggccggg ccgcctccgg cctggacgct cgatgccccg
     2341 ttcgcggcgc tggccctgct cgcgctgtgg ttcgccgccg ccgaactggc cgccctgcct
     2401 gccttgaaaa ggaagccgac atgatgatga catcgcaagt cctgctgtgg gtggcggtcg
     2461 tcatccttgg cgtcctggtc gcggcgctgg cccgccaggt cgggatcctg cacgaacgga
     2521 tcgcgcctgc cggcgcgctg acgctg
//
LOCUS       DASUDV010000402         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_411264,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000402 DASUDV010000000
VERSION     DASUDV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411264"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFV30_08680"
     CDS             complement(<1..761)
                     /locus_tag="VFV30_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX4848207.1"
                     /translation="MTLLQTIRPVTHDLGAFQVRRVLPSPQRIMVGPFVFVDQFGPAI
                     LPPGTAMDVRPHPHIGIATVTWLFEGAIDHRDSLGTLATIRPGQVNLMTAGRGIVHSE
                     RGLPSERDRAQPLYGMQTWLALPDGKEEIDPAFEAQTGLPLVEDGCASARVIMGTLWG
                     RTAPTTQHSPTIYAEINLAPGGQIPLDPEAEERAVMLVGGEASVDGVELELYSLAVLA
                     PGQSMRLGSQLGGRIMLLGGAALDSPRHVWWNFVSS"
     gene            complement(758..1030)
                     /locus_tag="VFV30_08685"
     CDS             complement(758..1030)
                     /locus_tag="VFV30_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX4848208.1"
                     /translation="MTTPLAAEIEALLTAAFAPTRLAVINDSARHHGHAGDDGSGESH
                     FTVEIESAAFAGVSRLQRQRLVNAALGDIPGQRVHALAIKARAPGE"
     gene            1069..1647
                     /locus_tag="VFV30_08690"
     CDS             1069..1647
                     /locus_tag="VFV30_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX4848209.1"
                     /translation="MKQDRFHGRVGSAERPCDRPGCPEPGEFRAPGPRGSSFDGPGDY
                     RWFCLDHVREFNAGYDWFEGMTAEEIIAAQNPIHGWDRQTRAFSPTAGIDSAPRWADF
                     SDPLEAIGARARSRKPAQRADGRAVTPDERRALDVLGLNLDATRHDLRSRYSQLVRKY
                     HPDRNGGDRSFEVRLQAVIEAYQLLRRAAAFA"
     gene            complement(1666..2106)
                     /locus_tag="VFV30_08695"
     CDS             complement(1666..2106)
                     /locus_tag="VFV30_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX4848210.1"
                     /translation="MYELSVTRLINAAPDKVWQVLTERTNEWWCPKPWRAEVNWGSRT
                     PGGRTHTTMYGPNGEKNEHPGFILAWDEGRRIAVTDAIEGDLQPAGPFMLGIWEIAPE
                     GTGTRYTGRARHWTPESMAHHKEMGFDEGWGIMADQFKELCEAG"
     gene            complement(2107..2553)
                     /gene="aac(6')"
                     /locus_tag="VFV30_08700"
     CDS             complement(2107..2553)
                     /gene="aac(6')"
                     /locus_tag="VFV30_08700"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: protein motif:HMM:NF043067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HEX4848211.1"
                     /translation="MQIVPACADHLAAWGEMRFALWPWDSPAEHATAAKADYLSGSPD
                     RAAFIVLDDAGSPVAFAEAALRRDYVEGCDTSPVAFLEGIYVAPAARLQGLARQLSDT
                     VAAWGRARGANEFASNALIDNTASHAFHAALGFAETERVVFFRKEL"
     gene            complement(2553..3203)
                     /locus_tag="VFV30_08705"
     CDS             complement(2553..3203)
                     /locus_tag="VFV30_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4848212.1"
                     /translation="MLQLFGHRFSSYTWKALIALYANETPFIFCGLGEGDAVAEALVA
                     SAGPSGQFPVLQDGERVVFETSVIIEYLDHHHPGAHRFIPVDPASALDVRMWDRVFDL
                     HVMAPMQAAVAHRIANPDCPVEEARGKSRAALTRSYRWIEGWLELYTQRDRITLVECA
                     AAPSLFYADWVIAIPAECPRLRAWRAHLLALPAVKRCVDDARQYRHFFPLGAPDRD"
     gene            complement(3208..3684)
                     /locus_tag="VFV30_08710"
     CDS             complement(3208..3684)
                     /locus_tag="VFV30_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4848213.1"
                     /translation="MTDATLCLWYDGTAEEAARFYAETFPDSRVGAIHRAPADFPGGK
                     EGDPLTVEFTVLGLNCIGLNGGPIFPHTEAFSIQVGTDSQEETDRYWNAIVGNGGAES
                     ACGWCKDKWGLNWQITPKVLTAGMADPDPAVRTRVFEAMMTMGKIDHAAIEAAIAG"
     gene            complement(3681..>4234)
                     /locus_tag="VFV30_08715"
     CDS             complement(3681..>4234)
                     /locus_tag="VFV30_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4848214.1"
                     /translation="ADYEQVLVDWAAKPAALLAANPMGKVPTVIHHAPGGDRVVTECA
                     AICAYLGDAHPEAGLAPTDAERADFYRWMFFAAGPVEQAIVGKSMGWDVPEGREGMVG
                     FGSFERTLGALEGHFAANAFVCGDRFTMADVYVGSQVDWGLTFGSIPPREAFVAYAER
                     LQARPAYKAAKGIDHALIEETRK"
BASE COUNT          688 a         1442 c         1428 g          676 t
ORIGIN      
        1 gaggaaacga agttccacca cacgtggcgc ggactgtcca gcgccgcgcc gcccagcagc
       61 atgatccgcc cgcccagctg gctgcccagc cgcatcgact gcccgggtgc aaggacggcg
      121 agcgagtaaa gctcaagctc caccccgtcg acgctggcct caccgccgac cagcatcacc
      181 gcgcgctctt ccgcttcggg atcgagcggg atctgcccgc caggggccag gttgatttcg
      241 gcatagatgg tcggcgaatg ctgggtggtc ggcgcggtgc gcccccacag cgtgcccatg
      301 atcacccgcg cgctggcgca gccgtcctcc accaacggca gcccggtctg cgcttcgaag
      361 gccgggtcga tttcctcttt gccgtcgggc agggccagcc aggtctgcat cccgtagagc
      421 ggctgcgcgc ggtcccgctc ggacggcagg ccgcgctcgg aatggacgat cccgcgcccg
      481 gcggtcatca ggttgacctg gccggggcgg atcgtcgcca aggtgcccag actgtcgcgg
      541 tgatcgatcg cgccttcgaa cagccaggtc accgtggcga tcccgatgtg cgggtggggg
      601 cgcacgtcca tcgccgtgcc gggcggcagg atcgccgggc cgaactggtc cacgaacacg
      661 aacggtccca ccatgatccg ctgcgggctg ggcagcaccc ggcggacctg aaacgcgccc
      721 agatcgtggg tcacggggcg gatcgtctga agcagggtca ttcccccggc gcccgcgcct
      781 tgatggccag ggcatggacc cgctggccgg gaatgtcgcc cagcgcggcg ttgaccaggc
      841 gctggcgttg cagccgcgaa accccggcaa aggccgcgct ttcgatctcc accgtgaaat
      901 ggctctcgcc cgatccgtca tcgccggcgt ggccgtgatg gcgggcgctg tcgttgatca
      961 cggccaggcg ggtgggggcg aaggcggcgg tcagcagggc ttcgatttcg gcggcgaggg
     1021 gtgtggtcat ggcttttctt ctagacgaac tgttgccatg gggaaggggt gaagcaggat
     1081 cgatttcacg gacgggtggg cagcgctgag cgcccttgcg accggcccgg ctgccccgag
     1141 ccgggggaat tccgcgcgcc cggaccgcgc ggctccagct ttgacgggcc gggcgattac
     1201 cgctggttct gcctggacca tgtccgcgaa ttcaacgccg gctatgactg gtttgaaggc
     1261 atgaccgcgg aagagatcat cgccgcgcaa aacccgatcc acggctggga ccgccagacc
     1321 cgcgcctttt cgccgaccgc tggaatcgat tccgcgccgc gctgggccga tttcagcgat
     1381 ccgctggagg cgatcggcgc ccgcgcgcgc agccgcaagc cggcccagcg cgccgatggc
     1441 cgggccgtga ccccggacga gcggcgcgcg ctggatgtgc tggggctgaa cctggatgcg
     1501 acccgccacg acctgcgaag ccgctattcg cagctggtcc gcaaatacca ccccgatcgc
     1561 aacggcggcg accgcagctt cgaagtgcgg ctgcaggcgg tgatcgaagc ctaccagctg
     1621 ctgcgccgcg ccgcggcctt tgcctgatcg ctcagccggg cgggcctagc cggcctcgca
     1681 cagttccttg aactggtcgg ccatgatccc ccagccttcg tcaaagccca tttccttgtg
     1741 gtgggccatg ctttccgggg tccagtggcg ggcccggccg gtgtagcggg tgccggtgcc
     1801 ttcgggcgcg atttcccaga tccccagcat gaagggcccg gccggttgca gatcaccctc
     1861 gatcgcatcg gtcaccgcga tccggcggcc ttcgtcccag gccaggatga aacccgggtg
     1921 ctcgttcttc tcgccgttcg gtccgtacat cgtggtgtga gtccgtccgc caggcgtgcg
     1981 gctaccccag ttgacttcgg cccgccacgg cttggggcac caccactcgt tggtccgctc
     2041 ggtcagcacc tgccagacct tgtcgggcgc ggcattgatc aagcgggtga cggacaattc
     2101 gtacattcaa agctccttgc gaaaaaagac cacgcgttcg gtttcggcaa agccaagcgc
     2161 cgcgtggaag gcatggctgg cagtgttatc gatcagcgca ttcgaagcaa attcgttcgc
     2221 gccgcgcgcg cggccccagg cggcaacggt gtcgctcagc tgtcgggcaa ggccctgcag
     2281 ccgcgccgcg ggcgccacat agatgccttc aaggaaggca acgggggacg tgtcgcaccc
     2341 ttccacatag tcgcggcgca gggcggcttc ggcaaaggcc accggactgc ctgcatcatc
     2401 aagcacgatg aaggcggcac gatccggcga tccggacagg tagtccgcct tggcggcggt
     2461 ggcgtgttcg gccgggctgt cccacggcca cagggcaaaa cgcatctcgc cccaggcggc
     2521 cagatgatca gcacaggcag ggacaatctg catcaatcgc gatccggcgc gccgagcgga
     2581 aaaaagtggc gatactggcg cgcatcatca acacagcgtt tcaccgcggg caaggccagc
     2641 agatgtgccc gccaggcgcg caggcgcggg cactcagccg gtattgcgat cacccagtcg
     2701 gcatagaaca ggctgggcgc agcggcgcat tccaccaggg tgatccggtc gcgctgggtg
     2761 tagagttcca gccagccttc gatccagcgg taggaccggg tgagggcggc ccgagacttg
     2821 ccgcgcgcct cttccaccgg gcagtccggg ttggcgatcc ggtgcgccac cgcggcctgc
     2881 attggcgcca tgacgtgcag gtcgaacacc cggtcccaca tccgcacgtc cagcgcgctg
     2941 gccggatcga ccgggatgaa gcggtgcgcc ccgggatggt gatgatcgag gtattcgatg
     3001 atcacgctgg tttcgaacac cacccgctcg ccatcctgca gcaccgggaa ctgaccgctg
     3061 ggcccggcgc tggccaccag cgcctcggcc accgcatcgc cttcgcccaa gccgcaaaag
     3121 atgaagggcg tttcattggc atagagtgcg atcaacgcct tccaggtgta gcttgaaaag
     3181 cggtggccaa agagttgcag cattcgatca accggcaatt gcggcttcga tcgcggcgtg
     3241 gtcgatcttg cccatggtca tcatcgcttc gaacacgcgg gtgcggaccg cgggatcggg
     3301 atcggccatg ccggcggtca gcaccttggg ggtgatctgc cagttcaggc cccacttgtc
     3361 cttgcaccag ccgcaggcgc tctccgcccc gccgtttccg acgatcgcgt tccagtaacg
     3421 gtcggtttcc tcctggctgt cggtgccaac ctggatcgag aaggcctcgg tgtgcggaaa
     3481 gatcggcccg ccgttcagcc cgatgcaatt gaggccgagc acggtgaatt ccaccgtcag
     3541 cggatcaccc tccttgccgc caggaaagtc ggccggcgcg cggtggatgg cgccaacccg
     3601 gctgtccgga aaggtttcgg catagaaccg cgcggcctct tcggcggtgc cgtcgtacca
     3661 caggcacagg gtggcatcgg tcacttgcgg gtctcctcga tcagggcatg gtcgatgccc
     3721 ttggccgctt tgtaggcggg ccgcgcctgg agccgttcgg cataggcaac gaaggcctcg
     3781 cgcggcggaa tcgagccgaa ggtcaggccc cagtcgacct ggctgccgac atagacgtcg
     3841 gccatggtga agcggtcccc gcagacgaag gcgttcgcgg cgaaatggcc ctcaagcgcg
     3901 ccaagcgtgc gctcgaagct gccgaagccg accatgccct cgcgcccctc gggcacgtcc
     3961 caacccatcg acttgccgac gatcgcctgc tcgaccgggc cggccgcgaa gaacatccag
     4021 cggtagaagt cggcccgctc ggcgtcggtc ggggcaagcc ccgcttcggg atgggcatcg
     4081 cccagatagg cgcagatcgc cgcgcattcg gtcaccaccc ggtccccgcc cggggcgtgg
     4141 tggatcacgg tcggcacctt gcccatcggg ttggcggcca gcagcgcagc gggcttggcg
     4201 gcccaatcga ccagcacctg ctcataatcc gcgc
//
LOCUS       DASUDV010000403         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1251036,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000403 DASUDV010000000
VERSION     DASUDV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251036"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFV30_08720"
     CDS             complement(<1..532)
                     /locus_tag="VFV30_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX4848215.1"
                     /translation="MVTKARPKQPPKPLGPARLEELALAYVARFATSAAKLDTYLRRK
                     LRERGWEGEGEPPVAALVSRFVAAGSVDDAAFARAKSGSLLRRGYGQRRIGQALHAAG
                     IGEDIREEVRPGEGEARRAALAMARKRRFGPFGTGAADRAVREKQIAALLRAGHRLDS
                     ARELVNAPSEAAALDWA"
     gene            672..>2330
                     /locus_tag="VFV30_08725"
     CDS             672..>2330
                     /locus_tag="VFV30_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX4848216.1"
                     /translation="MTDASVMEPNAAAGLVPTPNEDDLPRRFSDFATFGEALDYAAQG
                     KRGLNFHDPRGTLKRVYPFRELREDALAMARRLIAHGIGKDDRVALIAETGPEFAALF
                     CGCVYAGAWPVPLPLPTSFGGKEAYIDQLAVQLASSDPKILFYPAEIAAMTKAAADRQ
                     GCEGMTWEDFALQAQSEADLPQAQPDDICYLQYSSGSTRFPHGVAVTHASLLNNLAGH
                     GEGMKLIQSDRCVSWLPWYHDMGLVGCFLSVIANQVSTDYIKTEDFARRPLAWLDMIS
                     RNPGTTASYSPTFGYDICARRISSQSNVAERFDLSRWRIAGNGADMIRPDVMQGFVNA
                     FAPAGFKASAFLPSYGLAEATLAVTLMPPGEGIRVELVEEERLSGAQRDLSRPARYRA
                     IVNCGKAVKDMALEIRGAKGEVLGDHQIGKVWCKGPSVMVGYFRDPEATEACLVDGWL
                     DTGDMGYMVDGYLFIVGRAKDMIIINGKNHWPQDIEWAVEQLPGFNHGDIAAFSVETE
                     TGEESVAVLVHCRVSDPQERQKLHDTIRDKVRSILGHPAIVELVP"
BASE COUNT          372 a          793 c          785 g          380 t
ORIGIN      
        1 cggcccaatc cagcgcggcg gcttcgctgg gggcattgac caattcgcgg gcgctgtcca
       61 gccggtgccc ggcccgcagc agcgcggcga tctgcttttc gcgcaccgcg cggtcggcgg
      121 cgccggtccc gaacgggccg aagcgccgct tgcgggccat ggcgagcgcg gcgcggcggg
      181 cctcgccttc gcccgggcgc acttcctcgc ggatgtcctc gccgatcccg gcggcatgga
      241 gcgcctggcc gatccgccgc tggccgtagc cgcggcgcag caggctgccc gacttggccc
      301 gggcaaaggc ggcatcgtcg acagagccgg cggccacgaa ccggctgacc agcgcggcca
      361 ccggcggctc cccctccccc tcccagccgc gctcgcgcag cttgcggcgc aggtaggtgt
      421 ccagcttggc cgcgctggtg gcaaagcgcg cgacataggc cagcgccagc tcctccagcc
      481 gggccgggcc gagcggtttg ggcggctgtt tggggcgagc tttggttacc attggcctta
      541 ttcgtgccac actatgccgc gattgggaaa gcctgtgggt gtgggccggt ttggcccgca
      601 gagggcttaa atagacgtgc cgggccaagg gttgcggccc agcgcactga aaaagaacgg
      661 gttattcgcg catgactgac gcttccgtga tggagccgaa tgccgctgcc ggcctcgtgc
      721 cgacgccgaa cgaagacgat cttccccgcc gcttttccga tttcgccact tttggcgaag
      781 ccttggacta tgcggcgcag ggcaagcgcg ggctcaattt ccacgatccg cgcggcacgc
      841 tgaagcgggt ctatccgttc cgcgagctgc gcgaggacgc cctcgccatg gcccgccgcc
      901 tgattgccca cggcatcggc aaggatgacc gggtggcgct gatcgccgaa accggaccgg
      961 aatttgccgc gctgttctgc ggctgcgtct atgccggggc ctggccggtg ccgctgccgc
     1021 tgccgaccag ctttggcggc aaggaagcct atatcgacca gctggcggtg cagctcgcct
     1081 ccagcgatcc caagatcctg ttctacccgg ccgaaatcgc agcgatgacc aaggccgccg
     1141 ccgaccgcca gggctgcgaa ggcatgacct gggaagactt tgccctgcag gcgcagagcg
     1201 aggccgacct gccccaggcc cagcccgacg atatctgcta tctgcaatat tccagcggat
     1261 cgacccgctt cccgcacggc gtggcggtga cccatgccag cctgctcaac aacctggccg
     1321 gccacggcga gggaatgaag ctgatccaga gcgaccgctg cgtcagctgg ctgccgtggt
     1381 atcacgacat gggcctggtc ggctgcttcc tgtcggtgat cgccaaccag gtttcgaccg
     1441 attacatcaa gaccgaggat ttcgcccgcc gcccgctggc ctggctcgac atgatcagcc
     1501 gcaaccccgg cacgaccgct tcctattcgc cgactttcgg ttacgacatt tgcgcgcggc
     1561 gcatttccag ccagagcaac gtggccgaac ggttcgacct gtcgcgctgg cggatcgccg
     1621 gcaacggcgc cgacatgatc cgcccggacg tgatgcaggg cttcgtcaat gcctttgccc
     1681 cggccgggtt caaggccagt gccttcctgc cctcctatgg cctcgccgaa gcgacgctgg
     1741 cggtcacgct gatgccgccg ggcgaaggga tccgggtcga gctggtcgag gaagagcgcc
     1801 tgtccggtgc ccagcgcgac ctgtcgcgcc cggcgcgcta tcgcgcaatc gtcaactgcg
     1861 gcaaggcggt gaaagacatg gcgctggaaa tccggggcgc caagggcgaa gtgctgggcg
     1921 atcaccagat tggcaaggtg tggtgcaagg gtccgtcggt gatggtcggc tatttccgcg
     1981 atccggaagc gaccgaggcc tgcctggtcg acggctggct ggataccggc gacatgggct
     2041 acatggtcga tggctacctg ttcatcgtcg gccgcgccaa ggacatgatc atcatcaacg
     2101 gcaagaacca ctggccgcag gacatcgaat gggcggtgga acagctgccc ggcttcaacc
     2161 acggcgacat tgccgccttc tcggtcgaga ccgagacggg cgaggaatcg gtggccgtgc
     2221 tggtccactg ccgggtgtct gacccgcagg agcgccagaa gctgcacgac acgatccgcg
     2281 acaaggtccg ctcgatcctg ggccacccgg cgatcgtcga actggtcccg
//
LOCUS       DASUDV010000404         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_676370,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000404 DASUDV010000000
VERSION     DASUDV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676370"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFV30_08730"
     CDS             <1..957
                     /locus_tag="VFV30_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4848217.1"
                     /translation="PRGATGDGIAMAWRAGCRVANMEMMQFHPTCLYNLEVKNFLITE
                     AVRGEGGHLLNPRTGHRYMPDYDERAELAPRDIVARANDSEIKRDGLDYVHLDISHQP
                     PEFVREHFPNIHEKLLGLGIDMTRQPIPVVPAQHYTCGGIVIDLAGRTDLPGLYAAGE
                     CTESGLHGANRLASNSLLECFVFGEAAAADILDRWDRFDAPPPVRAWDESRVTDSDEE
                     VIIKQNWTEIRRFMWNYVGIVRTTKRLERAAHRITLLMEEVDDYYGHFRVSTDLIELR
                     NLLQCADLIVRSALERKESRGLHYTLDYPATLAEAKDTVLVP"
     gene            1019..2089
                     /gene="ppk2"
                     /locus_tag="VFV30_08735"
     CDS             1019..2089
                     /gene="ppk2"
                     /locus_tag="VFV30_08735"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX4848218.1"
                     /translation="MSHDSEDEAGGLPLRALGQGIAEERVVAAEQAAKAAIESLAARG
                     RWQEIEALIEGSAPDDAARMRALLGLPDQSIPLRQTSDELAPGWRLGGYPYKYKMLRK
                     DYERDKFLLQAELLKFQTWIKDTGARVVILFEGRDAAGKGGTIKRFMEHLNPRGARVV
                     ALEKPNEVERGQWYFQRYAAHLPTKGEIVLFDRSWYNRAGVEQVMGFCSQQEYRDFLR
                     EAPEFERNLVRGGIHLIKFWFSVSREEQLRRFTERKVHPLKQWKLSQIDLASLDKWDD
                     YTRAKEAMFAATDTEACPWIVIKSDDKKRARLNAMRFVLSAFPYAGKDPAVIGPVDAL
                     LVGRAHAIRERDEPAPPYSPQP"
     gene            2282..>2497
                     /locus_tag="VFV30_08740"
     CDS             2282..>2497
                     /locus_tag="VFV30_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848219.1"
                     /translation="MTAKRPAATVRARRKPGLAPAVEQSLADERAEAAIEAEEAALTE
                     IAKSGSDGVEAIIAGASPDDAARMRDYL"
BASE COUNT          468 a          852 c          829 g          348 t
ORIGIN      
        1 ccgcgcggcg ccaccggaga cggcatcgcc atggcctggc gcgcgggctg ccgcgtggcc
       61 aacatggaaa tgatgcagtt ccacccgacc tgcctctaca acctggaggt caagaacttc
      121 ctgatcaccg aggcggtgcg cggcgaaggc gggcacctgc tcaatccgcg caccgggcac
      181 cgctatatgc ccgattacga cgaacgggcc gagctggccc cgcgcgacat cgtggcccgc
      241 gccaacgaca gcgagatcaa gcgcgacggg ctcgattacg tccacctcga catcagccac
      301 cagccgccgg agttcgtccg cgagcatttc cccaacatcc acgaaaagct gctgggcctg
      361 gggatcgaca tgacccgcca gccgatcccg gtggtgcccg cgcagcatta cacctgcggc
      421 gggatcgtga tcgaccttgc cggccggacc gatctgcccg ggctctacgc cgcaggcgaa
      481 tgcaccgaaa gcggcctcca cggcgccaac cgcctggcca gcaacagcct gctcgaatgc
      541 tttgtcttcg gcgaggcggc ggcggccgac atcctcgacc gctgggaccg cttcgacgcc
      601 ccgcctccgg tccgcgcctg ggacgaaagc cgggtgaccg acagcgacga ggaagtgatc
      661 atcaagcaga actggacgga aatccgccgg ttcatgtgga actatgtcgg catcgtgcgc
      721 accaccaagc ggctggagcg cgccgcccac cggatcaccc tgctgatgga agaggtcgac
      781 gattattacg gccacttccg cgtttcgacc gacctgatcg agctgcgcaa cctgctgcaa
      841 tgcgccgatc tgatcgtccg ctcggcgctg gaacgcaagg aaagccgggg cctgcactac
      901 acgctggact atcccgccac gctggccgaa gcgaaggaca cggtgctggt cccgtgacgg
      961 caaccggcgc ggctgcaatc aaactgcaac aattgccccc cagcatcgcc gcgaagcgat
     1021 gagccacgac agcgaagacg aagccggggg gctgcccttg cgggcactgg gccaggggat
     1081 tgccgaggag cgcgttgtcg ctgccgagca ggccgccaag gccgcaatcg aatcgctggc
     1141 cgcgcgcggg cgctggcagg aaatcgaagc cctgatcgaa ggctccgcgc cggatgacgc
     1201 ggcccggatg cgtgccttgc tgggcctgcc ggaccagtcg atcccgctgc gccagaccag
     1261 cgacgagctg gcccccggct ggcggctggg cggatacccc tacaagtaca agatgctgcg
     1321 caaggattac gagcgcgaca agttcctgct ccaggccgag ctgctcaagt tccagacctg
     1381 gatcaaggac accggcgcgc gggtggtgat cctgttcgaa gggcgcgatg cggccggcaa
     1441 gggcggcacg atcaagcggt tcatggaaca cctcaacccg cgcggggcac gcgtggtggc
     1501 gctggaaaag cccaacgagg tggagcgcgg ccagtggtac ttccagcgct atgccgcgca
     1561 cctgccgacc aagggcgaga tcgtgctgtt cgaccgcagc tggtacaacc gcgccggggt
     1621 ggaacaggtc atggggttct gcagccagca ggaataccgc gacttcctgc gcgaggcacc
     1681 ggagttcgaa cgcaacctgg tccgcggcgg catccacctg atcaagttct ggttctcggt
     1741 cagccgcgag gagcagctgc gccgctttac cgagcgcaag gtgcacccgc tgaagcagtg
     1801 gaagctgtcg cagatcgatc tcgccagcct cgacaagtgg gatgactaca cccgcgcgaa
     1861 ggaggcgatg ttcgccgcca ccgataccga ggcctgcccc tggatcgtga tcaagagcga
     1921 cgacaagaag cgcgcccggc tcaacgccat gcgctttgtc ctgagcgcct ttccctatgc
     1981 cggcaaggat ccggcggtga tcggaccggt cgatgccctg ctggtcggcc gggcccacgc
     2041 gatccgcgag cgcgacgagc ccgccccgcc ctacagcccc cagccttgac ctgagcggct
     2101 cgttgttgcg cggaatgcaa cggcgcgtgc aagctatccg tttgactccg atggctggca
     2161 gcaggacatt ccgggcaagc cggaaagagc ctctgccccc tgcaagaccg cccttcccgg
     2221 ctaaccgaac gggaaggttt ggccgctagg ctgcggccgg aaagcaaggg agtcgatgtc
     2281 catgaccgca aagcgtcctg ccgccacggt ccgtgcgcgc cgcaagccgg gcctggcccc
     2341 ggcggtcgag cagagcctgg ccgacgagcg cgccgaagcc gcgatcgagg cggaggaggc
     2401 cgcgctgacc gagatcgcca agtcgggcag cgacggcgtg gaagcgatca tcgccggcgc
     2461 ctcgcccgac gatgcggcgc ggatgcggga ctatctg
//
LOCUS       DASUDV010000405         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_477971,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000405 DASUDV010000000
VERSION     DASUDV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477971"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFV30_08745"
     CDS             <1..447
                     /locus_tag="VFV30_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX4848220.1"
                     /translation="AVFLERVDIVASYAREVHSPSWSMQIPSVIALRQYVKPSEHPAF
                     TRFNLFLRDRFSCQYCGSPHNLTFDHVVPRRLGGRTSWENVATACAPCNLKKGGRTPK
                     QAGMPLLIEPIRPTSWQLQERGRAFPPGYLHETWRDWLYWDIELEA"
     gene            complement(448..1065)
                     /locus_tag="VFV30_08750"
     CDS             complement(448..1065)
                     /locus_tag="VFV30_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848221.1"
                     /translation="MTPWLIWGTAAALYLLFRLWYDNWRGPLTKGEIDAFFDEVHAKY
                     GGGNDPAVLRAFLEADDGKEFVMLNLVKVAEGMVADPDTGEAVDGRTMMRRYSDPFVK
                     ALLAKGGHPGMVGRKVGPYVDAWNVEADPGWSIFGLMRYRSRRDMIAMVRNPLFAKVH
                     PYKLLGIPTTFSFPTQREISVYMGPRVWVALVLALVAALVQIAAA"
     gene            complement(1065..>2550)
                     /locus_tag="VFV30_08755"
     CDS             complement(1065..>2550)
                     /locus_tag="VFV30_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4848222.1"
                     /translation="TYVTLDAKGELFAVASADFIIARKGYEKAADQPLRDLSEQLESQ
                     RFSQIQREYAHRFDAPVRIRNVHVFNPVTGARSAASDVVVSGNRIALVLPAGSASTPG
                     EAEIDGAGGTLVPGLYEMHGHLAQQDALMNLLAGITTVRDMGNTNPVLDVLIDRIGKG
                     EVAGPRIVRSGFIEGKSPFSSATGKLVESEAEAIEAVRWYAARGYWQVKLYNSMDPKW
                     APAVVAEAHRLGLRVAGHVPAFSSADAMIGAGFDEITHVNQLMLGWVLNPGEDTRTLF
                     RFTAMRRFPGLDLAAPRVTTTLDAMVAKNVAHEPTIGIHELGLTALDGRPNPGAIDYI
                     DHMPTAEQRQLKQALFGADTPKQRAEYVAAYGKIMDTLRLMHQRGILLIPGTDLGGGF
                     TYHRELELFGQLGMTPAQVLRRGTWDMARYMGQDQMLGSIERGKLADFFLVPGDPTVD
                     LKAIKRVAMVVKGGTVYFPDEIYPQLGIKPFAFVPKLTRPAGAR"
BASE COUNT          396 a          870 c          867 g          417 t
ORIGIN      
        1 gcggtgttcc tcgaacgggt cgatatcgtc gcctcctatg cgcgcgaggt gcattcgccc
       61 tcgtggtcga tgcagatccc cagcgtgatc gcgctgcgcc agtacgtgaa gcccagcgag
      121 cacccggcct tcacccgctt caacctgttc ctgcgcgacc ggttctcctg ccagtactgc
      181 ggatcgcctc acaacctgac cttcgatcac gtggtgcccc gccggctggg cggccggacc
      241 agctgggaaa acgtcgccac cgcctgcgct ccgtgcaacc tcaagaaggg cgggcgcacg
      301 cccaagcagg ccggcatgcc tctgctgatc gagccgatcc gcccgaccag ctggcaactc
      361 caggaacgcg gccgcgcctt cccgccgggc tatctccacg aaacctggcg cgactggctc
      421 tactgggaca tcgagctgga ggcctgatca ggccgccgcg atctgcacca gcgccgcgac
      481 cagggcgagg accagcgcga cccacacccg ggggcccatg tagaccgaaa tctcgcgctg
      541 ggtggggaag ctgaaggtcg tcgggatgcc cagcagcttg taggggtgga ccttggcgaa
      601 cagcgggttg cggaccatcg cgatcatgtc gcggcggctg cggtagcgca tcaggccgaa
      661 gatcgaccag ccgggatcgg cctcgacatt ccaggcatcg acataggggc cgaccttgcg
      721 cccgaccatg ccggggtgtc cgcccttggc cagcagggcc tttacgaagg gatcggaata
      781 gcgccgcatc atcgtgcggc cgtccaccgc ctcgccggtg tcgggatcgg ccaccatgcc
      841 ctcggccacc ttgaccaggt tgagcatgac gaattccttg ccgtcgtcgg cctccagaaa
      901 ggcgcgcagc acggcgggat cgttgccgcc gccatacttg gcgtgaacct cgtcgaagaa
      961 ggcgtcgatt tcccccttgg tcagcggccc gcgccagttg tcgtaccaca gccggaacag
     1021 cagatagagc gcggcggcgg tgccccagat cagccagggg gtcatcaccg tgcccccgcc
     1081 ggccgggtca gcttggggac aaaggcgaag ggcttgatcc ccagctgcgg atagatctcg
     1141 tccgggaagt agaccgtccc gcccttgacc accatggcca cccgcttgat cgccttgagg
     1201 tcaaccgtcg gatcgccggg gaccaggaag aagtcggcca gcttgccgcg ctcgatgctg
     1261 cccagcatct ggtcctggcc catgtagcgg gccatgtccc aggtgccgcg gcgcaggacc
     1321 tgggccgggg tcatgcccag ctggccgaac agctccagct cgcggtgata ggtgaacccg
     1381 ccgcccaggt ccgtgcccgg gatcagcagg atcccgcgtt ggtgcatcag ccgcagggtg
     1441 tccatgatct tgccataggc ggcgacatat tcggcgcgct gcttgggcgt atcggctccg
     1501 aacagggcct gcttcagctg gcgctgctcg gcggtgggca tgtgatcgat atagtcgatc
     1561 gcgcccgggt tgggccggcc gtccagcgcg gtcaggccca gttcgtggat gccgatggtc
     1621 ggctcgtgcg cgacgttttt ggcgaccatc gcgtcgagcg tggtggtgac ccgcggggcc
     1681 gccaggtcca gccccggaaa gcgccgcatc gcggtgaagc ggaacagggt gcgggtgtcc
     1741 tcgcccgggt tcagcaccca gcccagcatc agctggttga cgtgggtgat ctcgtcaaag
     1801 cccgcgccga tcatcgcgtc ggcgctggaa aaggccggaa cgtggccggc cacgcgcagc
     1861 cccagccggt gcgcctcggc caccacggcg ggggcccatt tggggtccat cgagttgtag
     1921 agcttgacct gccagtaccc gcgcgcggcg taccagcgga ccgcctcgat cgcctcggcc
     1981 tcgctttcca ccagcttgcc ggtggcgctg ctgaacgggc tcttgccttc gatgaagccg
     2041 gaccggacga tccgcgggcc ggccacttcg cccttgccga tccggtcgat cagcacgtcc
     2101 agcaccgggt tggtgttgcc catgtcgcgg accgtggtga tcccggccag caggttcatc
     2161 agcgcgtcct gctgggccag gtggccgtgc atttcgtaaa gcccggggac cagcgtgccg
     2221 ccggcgccgt cgatctccgc ctcgccgggg gtggaggcgc tgccggccgg aaggaccagc
     2281 gcgatccggt tgccggacac caccacgtcg ctggccgcgc tgcgcgcgcc ggtgacgggg
     2341 ttgaagacat ggacgttgcg gatccgcacc ggcgcgtcga aacggtgggc gtactcgcgc
     2401 tggatctggg aaaagcgctg cgattccagc tgctccgaca ggtcgcgcag cggctggtcg
     2461 gcggcctttt catagccctt gcgggcgatg atgaaatcgg cgctggccac ggcgaacagc
     2521 tcgcccttgg catccagcgt gacataggtg
//
LOCUS       DASUDV010000406         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1935740,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000406 DASUDV010000000
VERSION     DASUDV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935740"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFV30_08760"
     CDS             complement(<1..582)
                     /locus_tag="VFV30_08760"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX4848223.1"
                     /translation="MRAFVFPGQGSQKVGMGADLAGASAAAREVFEEVDEALGQKLSA
                     IMREGPEDVLTLTENAQPAIMANAIAVLRVLEKEGGITLADKGEFVAGHSLGEYTALC
                     AAGAFSLADTARLLKLRGHAMQAAVPVGVCAMCALLGADVAKAQALADAAAQGEVCTV
                     ANDNDPDQVVLSGHKAAIDRAIAMVKDFEIKRGV"
     gene            complement(611..1435)
                     /locus_tag="VFV30_08765"
     CDS             complement(611..1435)
                     /locus_tag="VFV30_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX4848224.1"
                     /translation="MLRVAICAPSRSIAEEDAARVLALAIDHPGLELHFHPQCYLSEG
                     HFAGSDEARAQAFVECANDPTFDAVWFARGGYGANRIAEAALAQLDKAAQNKTYLGYS
                     DTGYLLAGLYRERIGRPVHAPMPGDIRRAGGEAAVHRTLAWLGGDRSGEEPGGDGHPR
                     VAFNLTTLAMACGTPLMPGLAGHVVLIEEVSEHLYAIDRLFFHLTAHLGGIAGLRLGR
                     IGDVPQNDIDFGSGAEEIARHWCTRHAIAFLGSANIGHDIDNAIVPFGLAQGPAGA"
     gene            1488..2894
                     /locus_tag="VFV30_08770"
     CDS             1488..2894
                     /locus_tag="VFV30_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX4848225.1"
                     /translation="MSVLNSHPWFFCGIGGSGMLPLALILRGAGAAVAGSDRSRDQGR
                     TPEKFAWLESLGFALHPQDGSGITSAQQVLVASAAVEETVPEMIRAKELGCARMSRAE
                     LLSRLFNASPAGIAVGGTSGKSTVTGMIGWIMDRAGRDPTVMNGAVMKNYVAPDAPFA
                     SARVGHGGVFVSEVDESDGSIALYNPRVAVLGNVSLDHKSLEELRELFGAFLARAEVA
                     AVNFDDAEAAELAPHAQCLVSFAIDHPEAMLGVVPGTLVEAPTSLAATIEDRRDGSRI
                     PLHLKVPGRHNLANALAALAAANAAGVPLAEAVHALGTFAGLARRFDIVGTSAAGITV
                     IDDFGHNPDKVAATLRTLKAHPGRVIAFFQPHGYGPLRQMGAELAETFARELGPDDLT
                     ILCDPVYFGGTVDRSEGSERIVALIRGHGGQAQHIASRADCGAHIATIARAGDRVVIM
                     GARDDTLSQFARDVLASV"
     gene            complement(2891..>3383)
                     /locus_tag="VFV30_08775"
     CDS             complement(2891..>3383)
                     /locus_tag="VFV30_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEX4848226.1"
                     /translation="EIMAIALAEVASEPLLSRGLILALVAVVITVAVYGTVALIVKMD
                     DIGLHLVEKRSTAAEQKLGRGLLLAMPKLLGALSVIGTAAMLWVGGQIVIHGAHELGL
                     HGPSDLVHGIEHAVHDATGAAGGLLGWLTNATLSGIAGLALGAVIALVLHRVLKKEAG
                     ASA"
BASE COUNT          540 a         1190 c         1105 g          548 t
ORIGIN      
        1 gacgccgcgc ttgatctcga agtccttgac catggcaatg gcgcgatcga tcgccgcctt
       61 gtggcccgaa agcacgacct ggtcgggatc gttgtcgttg gcgacggtgc agacctcacc
      121 ctgggcagcg gcatcggcca gggcctgggc cttggccaca tcggcgccca gcagcgcgca
      181 catcgcgcac acgcccaccg gcaccgccgc ctgcatcgcg tgcccgcgca gcttcagcag
      241 cctggccgta tcggccagcg aaaaggcccc ggcggcgcac agcgcggtat attcgcccag
      301 gctgtgcccg gcgacgaatt cgcccttgtc ggcaagcgtg atcccgcctt ccttctccag
      361 cacccgcagc acggcaatgg cattggccat gatcgccggc tgggcatttt cggtaagggt
      421 cagaacgtct tcggggcctt cgcgcatgat cgccgagagc ttctggccca gcgcctcatc
      481 gacttcctcg aacacttcgc gcgcggcggc gctggccccg gcaaggtccg ctcccatgcc
      541 aaccttctgg ctgccctgtc ccggaaaaac gaatgctctc atctgctgtg tgctcctctg
      601 gcggcgccgg ttatgcgccc gccggtcctt gtgcaagtcc gaaggggaca atcgcgttgt
      661 cgatatcgtg gccgatgttg gcgctgccca ggaacgcgat cgcatggcgc gtgcaccagt
      721 gccgggcgat ctcttccgcg ccgcttccga aatcgatgtc gttttgcggc acatcgccga
      781 tccgccccag cctgagcccg gcgatgccgc ccagatgtgc ggtcaggtga aagaacagcc
      841 ggtcaatcgc atagagatgt tcggacacct cctcgatcag caccacgtgc cccgccagtc
      901 ccggcatcaa cggcgtgccg caggccatgg ccagggtggt gagattgaag gcaacgcgcg
      961 ggtgtccatc gccgccgggt tcctcgccgg atctgtcgcc gcccagccag gccagggtgc
     1021 ggtgcaccgc cgcctctccg cctgcccggc ggatgtcgcc cggcatcggc gcatgaaccg
     1081 gccggccaat ccgctcccga tagagcccgg cgagcagata gccggtatcg gaatagccca
     1141 gataggtctt gttttgcgcc gccttgtcca gctgggccag cgcggcctcg gcaatccggt
     1201 tggcgccgta tccgccgcgc gcgaaccaca ccgcatcgaa cgtgggatcg ttggcgcatt
     1261 cgacaaaggc ctgcgcccgc gcttcgtcgc tgccggcgaa atggccttcg ctgaggtagc
     1321 attgcgggtg aaagtgcaac tcaagcccgg gatggtcgat cgccagcgcc agcacgcggg
     1381 cggcgtcttc ttcggcaatc gaacgcgacg gggcacagat ggcaacgcgg agcatggttc
     1441 ttcctaaccc ggttgcgctg gctggcaagg gcgcataccg ggagaggatg agcgttctca
     1501 attcccaccc ctggttcttc tgcggcatcg gcggatcggg catgctgccg ctggcgctga
     1561 tcctgcgcgg cgccggggcc gccgtggccg gatccgatcg cagccgcgac cagggccgca
     1621 cgcccgaaaa attcgcctgg ctggaaagcc ttggctttgc actgcacccg caggacggca
     1681 gcgggatcac ctcggcgcag caggtgctgg tcgcctcggc agcggtcgag gagaccgtgc
     1741 ccgaaatgat ccgggcgaaa gagcttggct gcgcacggat gagccgggcc gagctgcttt
     1801 cgcgcctgtt caacgccagc cccgcgggca tcgcggtggg cggcaccagc gggaaaagca
     1861 ccgtcaccgg catgatcggc tggatcatgg accgcgccgg gcgcgacccc acagtaatga
     1921 acggcgcggt gatgaagaac tacgtcgcgc ccgatgcgcc ttttgccagc gcccgggtgg
     1981 gccatggcgg ggtgttcgtg tccgaagtgg acgaaagcga cggttcgatc gcgctttaca
     2041 accccagggt ggcggtgctc ggcaatgtca gcctcgacca caagagcctt gaggaactgc
     2101 gcgaactgtt cggcgccttc ctggcccggg ccgaggtggc ggcggtcaac ttcgacgatg
     2161 ccgaagcggc cgagcttgcc ccccatgccc agtgcctggt cagcttcgcg atcgatcacc
     2221 ccgaggcgat gctgggcgtg gtgcccggca ctctggttga ggcgccgacc agccttgccg
     2281 ccacgatcga ggaccggcgc gatggcagcc gcatcccgct gcacctgaag gttccgggcc
     2341 ggcacaatct ggccaatgcg ctcgccgcgc tggccgcggc caatgccgcc ggcgtgccgc
     2401 tggccgaggc ggtccatgcg ctgggcacct tcgcagggct ggcccgccgc ttcgacattg
     2461 tcggcacctc tgccgccggg atcacggtga tcgacgattt cggtcacaac cccgacaagg
     2521 ttgccgccac gctgcgcacg ctcaaagcgc accccggccg ggtgatcgcc ttcttccagc
     2581 cgcacggcta tggccccctg cggcagatgg gcgcggaact cgccgaaacg ttcgcccgcg
     2641 aactgggacc ggatgacctc acgatcctgt gcgatccggt ctacttcggc ggcacggtcg
     2701 atcgcagcga ggggagcgag cggattgtcg cgctgatccg cgggcacggc ggccaggccc
     2761 agcacatcgc ctcgcgcgcc gattgcgggg cgcacatcgc caccatcgcc cgcgcgggtg
     2821 accgggtggt gattatgggc gcacgcgacg atacgctgag ccagtttgcc cgcgatgtgc
     2881 tggccagcgt ctaggcgctg gcgccggctt ccttcttcag caccctgtgc agcaccaggg
     2941 cgatcaccgc gcccagcgcc agtccggcaa tccccgacag cgtggcattg gtcaaccagc
     3001 cgagcaggcc gcccgccgcg ccggtcgcat cgtgcaccgc gtgttcgatc ccgtgaacca
     3061 gatcggacgg cccatgcagc cccagttcgt gggcgccgtg gatcacgatt tgcccgccaa
     3121 cccacagcat cgccgccgtg ccgatcaccg aaagcgcgcc cagcaatttg ggcatcgcga
     3181 gcagcaggcc gcggcccagc ttctgttcgg cggcggtcga acgcttttcc accaggtgca
     3241 ggccgatatc gtccatcttc acgatcagcg cgaccgtgcc atagaccgca acggtgatca
     3301 ccaccgcaac cagcgccagg atcagccccc ggctgagcag cggctcactg gccacttcgg
     3361 ccagcgcgat cgccatgatt tcg
//
LOCUS       DASUDV010000407         5645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1493755,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000407 DASUDV010000000
VERSION     DASUDV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493755"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFV30_08780"
     CDS             complement(<1..647)
                     /locus_tag="VFV30_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX4848227.1"
                     /translation="MVSIATWNINSVRLRIDQVVRFLEQERPDVLCLQEIKTEEKHFP
                     HEAFEALGYTHRAVHGQKGYHGVATVSRIPFREQARNDWQANGEARHVGVELLGPGQG
                     LMLENVYIPAGGDIPDREANPKFGQKLDFLERMAAWSDRLDAPTLLVGDFNIAPLDCD
                     VYDHKALIKVVSHTPIEIEALWRLRDAHGWVDLGRKHIPAPERNWTWWSYRTFWQ"
     gene            complement(1066..1725)
                     /locus_tag="VFV30_08785"
     CDS             complement(1066..1725)
                     /locus_tag="VFV30_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX4848228.1"
                     /translation="MNSMKPSIRFFAGAAVSAALVVSAPHAFGLAAPAAAQTQSGDLA
                     QAVAALRGISTMRADFVQVDRAGKRLRGVMTLKRPGRIRFQYEKGVPFLIVSDGRALT
                     FVDYSVRQVQRWPITNSPLGALLDPKRDVMRYGKLQPVGNPNLVSVEVRDKAHPEYGV
                     ITLVFVRKASAPGGLELTSWAALDSQNSLTRVTLSNHQYGLAVPDTTFRFNDPRGTTR
                     R"
     gene            complement(1768..1935)
                     /gene="rpmG"
                     /locus_tag="VFV30_08790"
     CDS             complement(1768..1935)
                     /gene="rpmG"
                     /locus_tag="VFV30_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX4848229.1"
                     /translation="MAKPATVKIRLVSTADTGFFYVTKKNPRNTTEKMVFKKYDPVVR
                     KHVEFKEAKIK"
     gene            complement(2047..2844)
                     /gene="ppk2"
                     /locus_tag="VFV30_08795"
     CDS             complement(2047..2844)
                     /gene="ppk2"
                     /locus_tag="VFV30_08795"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX4848230.1"
                     /translation="MKKFKKKAFAKHYAPLEEELVAMARWAAKTGARIVVLFEGRDTA
                     GKGGAIKALAGPLNPRQCRTVALPKPSEQERSQWYFQRYAAHLPAAGEIVLFDRSWYN
                     RAGVEKVMGYCTPEQTEAFLAQVPTFEDLLVQDGILLFKYWLTTDQAEQEKRLAERLD
                     DPLKSWKLSPVDLAARAKYDDYTRAREAMLKATHTKTAPWTLVDFNDQRRGRLTLIRD
                     LLDRLPDTHVEPEPLELAPLEREPAEESFAVLGPIAPFEGKEKRAKG"
     gene            2949..3872
                     /gene="phhA"
                     /locus_tag="VFV30_08800"
     CDS             2949..3872
                     /gene="phhA"
                     /locus_tag="VFV30_08800"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX4848231.1"
                     /translation="MLETDFTRMPDPGDEVFTAPLKKPAQVGEDWLEPAQRAYTAEEN
                     AIWDELFARQMQVLPGRAASVFLAGLDKLDLGRGGVPDFGRLSEELGALTGWSVVPVP
                     MLIPDHVFFWHLANRRFPAGNFIRTRETFDYIQEPDVFHDVFGHVPMLTDPTYADYMQ
                     EYGRAGWKAMRYNRLKALGALYWYTVEFGLILEAEGLRAYGAGILSGPTEAVYCLEAE
                     SPNRIMLNVDRVMRTDYVIDDLQPTYFVIESFRELYHDTVERDFDRLYRSLAPGFTYA
                     NTAVIDIDDVLHRGTQEYALRGGRGSGAKPV"
     gene            complement(3896..4705)
                     /locus_tag="VFV30_08805"
     CDS             complement(3896..4705)
                     /locus_tag="VFV30_08805"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX4848232.1"
                     /translation="MDTIVTAILLGIVEGVTEFIPVSSTGHLILATEIFGYDAKAWAA
                     FNVVIQLGAILAVVVQYWRTFWAVGTGLLRLEPMSIAFIRNILLAFLPSAVLGFLLKD
                     YIDIMLGSPMIVGWALIAGGIAILAIEKWAKPGGPSGVAQLPVRQALGVGLFQCISMI
                     PGVSRSGATIMGALAMGIERRTAAEFSFFLAIPTMLGATTLELWDNHAELMAGTGPVG
                     WTEIAVGFAVSFVVALAVIRAFVAFVSRSGFAPFAWYRIVAGTLAVWLLWP"
     gene            complement(4705..>5645)
                     /locus_tag="VFV30_08810"
     CDS             complement(4705..>5645)
                     /locus_tag="VFV30_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4848233.1"
                     /translation="TIVAIVITSGVVAGAFGALTEVYGDLLRGAGAASRLAELLSERP
                     SIAAPDRPLQLPSPPRGKLSFENVTFRYPTRLEVAALADFSLTIEPGETVAIVGPSGA
                     GKSTLFQLAERFYDPQAGTIKLDGVPLTAVDPAEVRRRMALVPQEGVLFAASARDNLR
                     YGNWEASEEAIWDAARAANAEGFLRDLPDGLDTFLGEGGARLSGGQLQRIAIARAVLR
                     DAPILLLDEATSALDAESEQLVQQALDRLMANRTTLVIAHRLATVRNADRIVVMDQGR
                     IAETGTHDQLLKAKGLYARLAALQFDERAFAAQGTN"
BASE COUNT          950 a         1873 c         1890 g          932 t
ORIGIN      
        1 tcctgccaga aggtgcgata ggaccaccag gtccagttgc gttccggcgc gggaatatgc
       61 ttgcggccca gatcgaccca gccatgggca tcgcgcagcc gccacagcgc ttcgatctcg
      121 atgggggtgt ggctgaccac cttgatcagc gccttgtgat cgtaaacgtc gcagtccagc
      181 ggggcgatgt tgaaatcgcc aaccagcagg gtgggcgcat ccagcctgtc cgaccaggcc
      241 gccatccgct ccaggaaatc cagcttctgg ccgaacttgg gattggcctc acggtccggg
      301 atgtcgccgc cggccgggat gtagacgttc tccagcatca ggccctgccc ggggccgagc
      361 agctccaccc ccacgtgccg cgcctcgccg ttggcctgcc aatcgttgcg ggcctgttcg
      421 cgaaagggaa tccggctgac cgtggccacg ccgtgatagc ccttctggcc gtgaaccgcg
      481 cggtgggtgt agcccagcgc ctcgaaggct tcgtgcggga agtgcttttc ctcggtcttg
      541 atctcttgca ggcacagcac gtcgggccgc tcctgctcca gaaagcggac gacttgatca
      601 atccgcaggc ggaccgaatt gatgttccag gtggcgatag agaccatggc tagggcttta
      661 aaccggaatc gccgtcccgc aacagcggag gcgcggggtg gtcgagattt ggctcagggt
      721 ggagcgagaa tggtgccggg tgagaaccgg agcggagcgg cctttcgggc cgtgagcacc
      781 ggaagcgcaa accggtgccg ttcgcagccc gccctggggc gaatatcggc cgcccaaaag
      841 aaaaccctcg gtccgggggc attggaccga gggttccttt gtgcgttcgt cacgcttgac
      901 ggtctggccc ttgcggaggc ttgggcaaca ggggggaaac ccgcctcagg ccgcgccgtc
      961 gaacaaagat ttagggcagt ttgccttgct cggccatgaa caatcgcgcg ggatgtgtcg
     1021 caaattgtcg caactccggc acggcccacg acaggccgtt caccctcatc gacgagtggt
     1081 accgcgcggg tcgttgaagc ggaacgtggt gtccggcacg gccagcccgt actggtgatt
     1141 cgacagggtg acgcgggtca gcgaattctg cgagtcgagc gcggcccagc tggtcagctc
     1201 aagcccgccg ggggccgaag ccttgcgcac gaagaccagg gtgatcaccc cgtattcggg
     1261 atgggccttg tcgcgcactt caacgctgac caggttcgga ttgccgaccg gctggagctt
     1321 gccatagcgc atcacgtcgc gcttgggatc gagcagcgcg cccagcggac tgttggtgat
     1381 cggccagcgc tgaacctggc ggaccgagta atcgacgaag gtcagggccc ggccgtccga
     1441 gacgatcagg aagggcacgc ccttttcgta ctggaagcgg atccggcccg ggcgcttcag
     1501 ggtcatcacc ccgcgcagcc gcttgccggc gcggtccacc tggacgaaat cggcgcgcat
     1561 cgtgctgatg ccgcgcagcg cggcaacggc ctgggccaga tcgccggact gggtctgggc
     1621 cgcagccggc gcggccaggc caaacgcgtg gggggcggaa acgacaagcg ccgccgaaac
     1681 ggcggcacct gcgaaaaagc ggatcgaagg tttcatgctg ttcatgggcc gggtcttagc
     1741 cgcgctgcct tgaatagccg gtgaacctta cttgatcttg gcttccttga actcgacatg
     1801 cttgcgcacg acgggatcgt acttcttgaa caccatcttc tcggtggtgt tgcgcgggtt
     1861 cttcttggtc acatagaaga agccggtgtc ggcggtcgac acgaggcgga tcttgacggt
     1921 tgcgggcttc gccatggcgc ttgtcctgaa ctttcgattc gggcgctcgg cccttgaaaa
     1981 actgcgaggg cggcacgcaa gcgccgcccc ggaaggccgc gccaatgcgt cagcgcgggc
     2041 gcaaagtcaa ccctttgcgc gcttctcctt gccctcgaac ggcgcgatcg ggcccagcac
     2101 ggcaaagctc tcttcggccg gttcgcgctc caacggcgcc aactcgagcg gttcgggctc
     2161 gacatgggtg tcgggcagcc gatccagcag gtcgcggatc aaggtcagcc ggcctcggcg
     2221 ctggtcgttg aaatcgacca gggtccaggg cgcggtcttg gtgtgggtgg ccttcagcat
     2281 cgcctcgcgc gcgcgggtgt aatcatcgta cttcgcgcgc gcggccagat cgaccgggga
     2341 cagcttccag ctcttcagcg ggtcgtccag ccgctcggcc aggcgctttt cctgctcggc
     2401 ctggtcggtc gtgagccagt acttgaacag caggatcccg tcctggacca gcaggtcctc
     2461 gaacgtcggg acctgggcca ggaaggcctc ggtctgctcg ggcgtgcaat agcccatgac
     2521 cttttccacc ccggcgcggt tgtaccagct gcggtcgaac agcacgatct cgcccgcggc
     2581 cggcaggtgc gccgcatagc gctggaaata ccattggctg cgttcctgct cgctcggctt
     2641 gggcagggcc accgtgcggc actggcgcgg gttgagcggc ccggccaggg ccttgatcgc
     2701 cccgcccttg cccgccgtgt cgcgcccttc gaacaggacg acgatccgcg ccccggtctt
     2761 ggccgcccag cgcgccatgg ccaccagctc ttcctcgagc ggggcatagt gcttggcgaa
     2821 ggcttttttc ttgaactttt tcatggccga cgattccctt tcgcggcaac cctgcgcgtc
     2881 tcggcccgcc caatcaagcc gcgccatcgc gcttgctgtc ccgctttgga atggttacaa
     2941 ctgcaaccat gttggaaacc gactttaccc gcatgcccga ccccggcgac gaggtgttca
     3001 ccgcgccgct gaagaagccc gcccaggtgg gcgaagactg gctggagccg gcgcagcgcg
     3061 cctacaccgc cgaagaaaac gcgatctggg acgagctgtt cgcccgccag atgcaggtcc
     3121 tgcccgggcg cgcggccagc gtgttcctgg ccgggctcga caagctggac ctcggccgcg
     3181 gcggcgtgcc cgatttcggg cggctgtccg aagaactcgg cgcactgacc ggctggtcgg
     3241 tcgtcccggt gccgatgctg atccccgatc acgtgttctt ctggcacctg gccaaccgcc
     3301 gctttccggc cggcaacttc atccgcacgc gcgagacgtt cgactatatc caggaacccg
     3361 acgtcttcca cgacgtgttc gggcacgtgc cgatgctgac cgacccgacc tatgccgatt
     3421 acatgcagga gtacggccgc gccgggtgga aagcgatgcg ttacaaccgg ctgaaggcgc
     3481 ttggcgcgct ctattggtac acggtcgaat tcggcctgat cctcgaggcg gaagggctgc
     3541 gggcctatgg cgccgggatc ctctccggcc cgaccgaagc ggtctattgc ctggaggcgg
     3601 agagccccaa ccggatcatg ctcaacgtcg accgggtgat gcggaccgac tatgtgatcg
     3661 atgatctgca gccgacctat ttcgtgatcg aaagcttccg cgagctttac cacgacaccg
     3721 tggagcgcga tttcgaccgg ctgtaccgca gcctggcccc cggcttcacc tatgccaaca
     3781 ccgcggtgat cgacatcgac gacgtgctgc accggggcac acaggaatac gccctgcgcg
     3841 gcgggcgcgg cagcggggcc aagccggttt aggcgaaggc tgagcggggc tcagctcagg
     3901 gccagagcag ccacaccgcc agcgtgccgg cgacgatccg gtaccaggcg aagggggcaa
     3961 agcccgagcg gctgacgaag gccacgaaag cgcggatcac cgccagggcg accacgaagc
     4021 tgaccgcgaa accaaccgcg atctcggtcc agccgaccgg gccggtgccg gccatcagct
     4081 cggcgtggtt gtcccacagt tccagcgtgg tggcgcccag catggtcggg atcgcgagga
     4141 agaagctgaa ttcggccgcg gtgcggcgct cgatccccat cgccagcgcg cccatgatcg
     4201 tcgcgcccga acggctgacg ccggggatca tcgaaatgca ctggaacagg ccgacgccca
     4261 gcgcctggcg gaccggcagc tgcgccaccc cgctggggcc gccgggcttg gcccattttt
     4321 cgatcgccag gatcgcgatg ccgccggcga tcagcgccca gccgacgatc atcgggctgc
     4381 ccagcatgat gtcgatatag tccttgagca ggaaaccgag caccgccgag ggcaggaacg
     4441 cgagcaggat gttgcggatg aaggcgatgc tcatcggctc cagccggagc aggccggtgc
     4501 cgaccgccca gaaggtccgc cagtactgca ccaccacggc caggatcgcg ccgagctgga
     4561 tcaccacgtt gaacgcggcc caggccttgg cgtcatagcc gaagatctcg gtcgccagga
     4621 tcaggtgccc ggtcgaggag accggaatga attcggtgac cccttcgaca atgccgagca
     4681 ggatcgcggt gacaatcgta tccatcagtt cgtcccctgc gcggcaaagg cgcgctcatc
     4741 gaattgcagc gcggccagcc gggcgtaaag ccccttggcc ttgagcagct gatcgtgggt
     4801 gccggtttcg gcgatccggc cctggtccat caccacgatc cggtccgcgt tgcgcacggt
     4861 cgccaggcgg tgggcgatga ccagggtagt gcggttcgcc atcagccggt caagcgcctg
     4921 ctggaccagc tgctcgcttt cggcatcgag cgccgaggtc gcctcgtcga gcagcaggat
     4981 cggcgcatcg cgcagcaccg cgcgggcaat cgcgatccgc tggagctgcc cgcccgaaag
     5041 ccgggccccg ccctcgccca ggaaggtgtc gagcccgtcg ggcagatcgc gcaggaagcc
     5101 ttcggcgttg gcggcgcggg cggcgtccca gatcgcctct tcgctggcct cccagttgcc
     5161 atagcgcagg ttgtcgcggg ccgaggcggc aaacagcacg ccttcctgcg ggaccagcgc
     5221 catgcggcgg cgcacttcgg ccggatcgac cgcggtcagc ggcacgccgt ccagcttgat
     5281 ggtcccggcc tgaggatcat agaaccgctc ggccagctga aacagggtgg acttgccagc
     5341 gcccgaaggg ccgacaatcg ccaccgtctc acccggttcg atcgtcagcg agaaatcggc
     5401 cagcgcggcg acttccagcc gggtcggata gcggaaggtg acgttttcaa agctcagctt
     5461 gccgcgcggc gggctgggca gctgcagggg ccgatcgggc gccgcgatgc tgggcctttc
     5521 gctgagcagc tcggccaggc ggctggccgc cccggccccg cgcagcagat cgccgtaaac
     5581 ctcggtcagc gcgccaaacg ccccggccac caccccggag gtgatgacga tcgccacgat
     5641 cgttc
//
LOCUS       DASUDV010000408         8824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_632329,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000408 DASUDV010000000
VERSION     DASUDV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632329"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFV30_08815"
     CDS             <1..291
                     /locus_tag="VFV30_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848234.1"
                     /translation="PRARGRFVHIETVQPRRFLPGATSRGETHGPKPGFTREQYRMLA
                     ALYVYVSARAGRWLIPAQHNTVDSTIPQAHDDPQNFDLKAFGRELERFVKVR"
     gene            complement(288..611)
                     /locus_tag="VFV30_08820"
     CDS             complement(288..611)
                     /locus_tag="VFV30_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848235.1"
                     /translation="MFIILGIAIALGIPALIAAAVAFAMPTLAPGWSLRRRSVVAALI
                     AGFLPMTIPVIAALSDPANPAVFASVSGILFGGVLVALVIGLPVALLVGRRRGGPAVD
                     PTAFD"
     gene            complement(629..1138)
                     /gene="rimM"
                     /locus_tag="VFV30_08825"
     CDS             complement(629..1138)
                     /gene="rimM"
                     /locus_tag="VFV30_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070962.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4848236.1"
                     /translation="MRGNRPITLAAIAGAHGVAGEVRLKLFGEGVESLKRFRAFNDSA
                     LTLTKLRDDGKGGAIARFAEVADRTAAEKLRGTALTVPRAALPPLGEGEYYHADLLGL
                     AAVSTEGEALGQVIAVDNFGAGDVLEIERPGVDGKRGKRFMVPMRPEAVPEWDDERLV
                     IAAAFAADD"
     gene            complement(1142..1657)
                     /gene="rpsP"
                     /locus_tag="VFV30_08830"
     CDS             complement(1142..1657)
                     /gene="rpsP"
                     /locus_tag="VFV30_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4848237.1"
                     /translation="MSVSLRLSRGGSKKRPYYKVVVANSRAPRDGKYLEQVGTYNPLL
                     AKDDENRVRLVEDRVRYWLGVGAQPTDRVARMLDKAGIKERAATVNPKKGEPGQKAKD
                     RAEEKAEKAREAEEAAAAAAAAPAVEEVVEEAAAEAPAADEAAAEAPAAEEAAAEEAP
                     AAEASEEQAEG"
     gene            complement(1696..3147)
                     /gene="ffh"
                     /locus_tag="VFV30_08835"
     CDS             complement(1696..3147)
                     /gene="ffh"
                     /locus_tag="VFV30_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX4848238.1"
                     /translation="MFDALSDRLGTVFDRLRGRGALNEQDVRDAMREVRIALLEADVA
                     LPVVRRFIDEVTEKAVGQSVLKSVTPGQQVVKIVNDALVDMLGGEDVPGLDLNAAPPV
                     VIMMVGLQGSGKTTTTAKLGKLLKEKYGKKALMASLDVNRPAAQEQLKVLGEQVGVST
                     LPIIPGQQPTEIATRALQAAKLQAIDVLLLDTAGRLHVDQALMDEMRAVAGISAPKEV
                     LLVVDSLAGQDAVNVAKSFTEQVDLTGVVLTRMDGDARGGAALSMRHVTGKPIKFAGV
                     GEKLDALEVFQPSRVASRILGMGDVVSIVEKAAQAIEQEEAERMAERMAKGQFDMNDL
                     RSQLRQMQKMGGLGALAGMMPGMKKAKAAMQASGMDDRVLLRMDAIIGSMTPKERAKP
                     DLLNAKRKIRIAKGSGVQVQDVNKLLKMHQEMERAMKQIKKMGGLAGLGKLFGKGGLG
                     AAMPGLGGGGGGGLPGLGGPGMPPGLDDILKKK"
     gene            complement(3235..3310)
                     /locus_tag="VFV30_08840"
     tRNA            complement(3235..3310)
                     /locus_tag="VFV30_08840"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3275..3277),aa:Ala,seq:ggc)
     gene            complement(3409..3729)
                     /locus_tag="VFV30_08845"
     CDS             complement(3409..3729)
                     /locus_tag="VFV30_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HEX4848239.1"
                     /translation="MVKAEWGTKHGCPKCGTRFYDLGKDDPVTCIECGYAWHPEPVLK
                     SKQPIPYEEIQKEKVEEVADVDLADTDLDIDEDGDSPDNDVDLGGDDDLGVGLDDGES
                     PDDN"
     gene            3932..5233
                     /gene="aroA"
                     /locus_tag="VFV30_08850"
     CDS             3932..5233
                     /gene="aroA"
                     /locus_tag="VFV30_08850"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX4848240.1"
                     /translation="MATGPLKGMIRVPGDKSISHRSIMLGALAVGETRVTGLLEGEDV
                     LATAAAMRAMGASVERVGEGEWSVHGVGVGALLQPEAPLDMGNSGTSTRLLMGLVASH
                     AIETRFFGDASLSKRPMGRVIDPLSQMGAAFEASEGGRLPLTVRGANPAVPIRYRLPV
                     ASAQVKSAILLAGLNTPGITTVIEPVPTRDHSERMLRGFGAELEVETDSAGVRTIRIR
                     GEAELKPQTIEVPGDPSSAAFFLVAALVVPGSELTIQNVGLNPTRAGIIEVLRQMGGD
                     ITELNRRDVGGEPVADLLVRHSALKGIAVDPAVVPSMVDEFPVLFVAAALAEGTTVTT
                     GLDELRVKESDRITVMAEALTLAGARVTETEDGLTIHGTGGEPLRGSANSRVKTQLDH
                     RIAMSMAVAGLASRDGVEVDDTRPIATSFPVFEDLLAEVTQ"
     gene            5230..5757
                     /locus_tag="VFV30_08855"
     CDS             5230..5757
                     /locus_tag="VFV30_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848241.1"
                     /translation="MIRLLAVSALLLTAAAPGLPYPSYGASALQRKADLSISAIPIDT
                     PLAKVKGRLTKVPGSCEADGNCEWRDKAGVRHYFWGDVKPSPLVIKFVHAKDFRGRPI
                     RALGIGMARSKPAVIKAVKAFDPRIKLDCDRARVSGNVGPQECSFTTNPGWIQIGFDR
                     NDQLTMIRFDGYQFT"
     gene            5754..6380
                     /locus_tag="VFV30_08860"
     CDS             5754..6380
                     /locus_tag="VFV30_08860"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="d(CMP) kinase"
                     /protein_id="HEX4848242.1"
                     /translation="MIIAVDGPTASGKGTIAKALAAHFGLPHLDTGLLYRAVGRQCAL
                     NGGDADNPGDALSACQFPDSLLDDPELRSEATGGLASRVSVHPAVRQALFERQRAFAT
                     QPGGAVLDGRDIGTVIAPEAEVKLFVVASVPARAERRFKEMQAQGRSVSLAEIAADLE
                     ARDARDRSRVEAPLVAAPDAVLLDTSDLGRDEAIAAAIALAGAKCPKA"
     gene            6365..6721
                     /locus_tag="VFV30_08865"
     CDS             6365..6721
                     /locus_tag="VFV30_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848243.1"
                     /translation="MPEGLSALLLGWGLDWAWFKDALERALGVSSDALHVIVGVTLQL
                     ALVALLRTTLARIAPWALVLALTLLNEWNDFRVERWGDTALQWGEAAKDVGLTMLLPT
                     VLLLLARWRPGLFGLR"
     gene            6783..7142
                     /locus_tag="VFV30_08870"
     CDS             6783..7142
                     /locus_tag="VFV30_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4848244.1"
                     /translation="MIKLAFAAAAVILIAACATVPAKPRVALTEQEARGYAFAKNNCA
                     ACHGVVANASSPNPEAPPFEAVVNTKGLTARTLETFLRDSHNFPGAMAFAVEPAKVDD
                     ITAYMLTLRKPGYKPGI"
     gene            complement(7124..7462)
                     /locus_tag="VFV30_08875"
     CDS             complement(7124..7462)
                     /locus_tag="VFV30_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848245.1"
                     /translation="MTRNAPIHRPGLPPSWRLALWGGIAGVLALPAVAMQFSREVNWG
                     PGDFLAAALLLGLTGLGLELAARAPLSRHLRIAAALALVVMLLLIWAELAVGIIPQAA
                     ASPRLQIPGL"
     gene            7622..8569
                     /locus_tag="VFV30_08880"
     CDS             7622..8569
                     /locus_tag="VFV30_08880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848246.1"
                     /translation="MRDVIAEMGPSFLASRLKRLAERMQAGAASVIADAGLPLQPGHM
                     AVLAALRTGPKTVGQLADVTGTSQPGITRTVKQLVELGIVGNASREDQRSRAVMLTAA
                     GEEAARIAGTDIWPRVGQAAEQLLAGCGSDFLHEVARIEAALNEASIAARAAALEPGG
                     LRLREFDDSLAGAFHDINAEWISTMFALEQTDRDVLEQPRARIIDGGGTILFVEAPGL
                     GIVGTCALQKTGPRSFELTKMGVREVARGLKAGEFLLEKTIARAREMGADPLYLLTNR
                     KCAAAIHLYEKLGFAHDAGIMADYGARYQRCDVAMRYTG"
BASE COUNT         1440 a         3072 c         2873 g         1439 t
ORIGIN      
        1 ccgcgcgcgc gggggcgctt cgtccatatc gaaacggtcc agccgcggcg ctttctgccc
       61 ggcgccacca gccggggtga aacccacggg cccaagcccg gcttcacccg cgaacagtac
      121 cggatgctgg ccgcgcttta cgtctacgtc agcgcgcggg ccggccgctg gctgatcccg
      181 gcgcagcaca acaccgtgga ttcgaccatc ccccaagccc acgacgatcc gcagaacttc
      241 gatctgaaag ccttcgggcg ggaactcgag cggtttgtga aggtgcgtta gtcgaacgcg
      301 gtgggatcga ccgctgggcc accccgccgc cgtccaacca acagggccac cggcaggccg
      361 atcacaagcg cgaccagcac gccgccaaag aggatgcccg atacggaagc gaacaccgcc
      421 ggattggcag gatcgctcag cgcggcaatc accgggatcg tcatcggcag gaacccggcg
      481 atcagcgcgg ccaccacgct gcggcggcgc aggctccagc cgggggccag tgttggcatg
      541 gcaaaggcca ccgcggctgc gataagcgcc ggaatgccca gcgcaatcgc tattcccagg
      601 atgatgaaca tggccggagc ctagcgcact aatcgtcggc ggcaaaggcg gcggcgatga
      661 ccagccgctc gtcatcccat tcgggcacgg cctcgggccg catcggcacc atgaagcgct
      721 tgccgcgctt gccgtccacg ccggggcgtt cgatctccag cacgtcgccc gcgccgaaat
      781 tgtcgaccgc gatcacttgg cccagggcct cgccctcagt cgagaccgcg gccaggccga
      841 gcaggtcggc gtggtagtat tcgccctcgc ccaaaggggg cagcgcggcg cgcggcacgg
      901 tgagcgcggt gccgcgcagc ttttcggccg ccgtccggtc ggccacttcg gcaaagcggg
      961 caatcgcccc gcccttgccg tcatcgcgca gcttggtcag cgtcagcgcg gaatcgttga
     1021 aggcgcggaa tcgcttgagc gactccacgc cttcaccgaa cagcttgagc cggacttcgc
     1081 ccgccacgcc gtgcgcaccc gcaatggcgg ccagcgtgat ggggcgattc ccgcgcaagg
     1141 gttagccttc ggcctgctct tcgctggctt cggcagccgg agcttcctcg gcagcggctt
     1201 cttccgcggc gggggcttcg gcagcagctt cgtccgcggc aggcgcttcg gcagcggctt
     1261 cttcaaccac ttcttcaacc gccggagcag cggcggcagc ggccgcggct tcttccgctt
     1321 cacgggcctt ttcggccttt tcttcggcgc ggtccttggc cttctggccc ggctcaccct
     1381 tcttcgggtt cacggtggcg gcgcgctcct tgatcccggc cttgtcgagc atgcgggcga
     1441 cgcggtcggt cggctgcgcg ccaacgccca gccagtagcg gacgcggtct tcgaccagac
     1501 ggacgcggtt ttcatcgtcc ttggccagca gcgggttgta ggtgccgacc tgctccaggt
     1561 acttgccgtc gcgcggggca cggctgttgg caacgacgac cttgtagtac gggcgcttct
     1621 tcgagccacc acgcgaaaga cgaagggaaa cagacatttg aactaccttt ctagcaaatt
     1681 gaattaagcg ttaaatcact tctttttcag aatatcgtcg agccccgggg gcatgcccgg
     1741 accgcccagc ccgggaaggc cgccaccgcc gccgccgcca aggcccggca tcgcggcacc
     1801 caggccgccc ttgccgaaca gcttgcccag cccggccagg ccgcccatct tcttgatctg
     1861 cttcatcgcg cgctccattt cctggtgcat cttgagaagc ttgttcacgt cctggacctg
     1921 cacgcccgaa cccttggcga tgcggatctt gcgcttggcg ttgagcagat cgggcttggc
     1981 ccgctccttt ggcgtcatgg agccgataat cgcgtccatg cgcagcagca cccggtcgtc
     2041 catgccgctg gcctgcatcg cggccttggc cttcttcatg cccggcatca tgccggccag
     2101 cgcgcccagg ccgcccatct tctgcatctg gcgaagctgg ctgcgcaggt cgttcatgtc
     2161 gaactgaccc ttggccatgc gctcggccat gcgctcggct tcttcctgct cgatcgcctg
     2221 ggcagccttc tcgacgatcg agacgacatc gcccatgccg aggatccggc tggcgacccg
     2281 gctgggctgg aacacttcca gcgcgtccag cttttccccg acaccggcga acttgatcgg
     2341 cttgccggta acgtgccgca tcgacagcgc cgcaccgccg cgggcatcgc cgtccatccg
     2401 ggtcagcacg acgccggtca gatcaacctg ttcggtgaac gacttggcga cgttgaccgc
     2461 gtcctggccg gccagcgaat cgaccaccag caggacttcc ttgggcgccg agatgcccgc
     2521 cacggcgcgc atttcgtcca tcagcgcctg atcgacgtgg agccggccgg cggtgtcgag
     2581 cagcaggacg tcgatcgcct ggagcttggc cgcctgcagc gcgcgggtgg cgatctcggt
     2641 cggctgctgg ccgggaatga tcggcagggt ggaaacgcca acctgctcgc ccagcacctt
     2701 cagctgttcc tgcgcggccg gacggttgac gtcgagcgac gccatcaacg ccttcttgcc
     2761 gtacttttcc ttgagcagct tgcccagctt ggccgtggtc gtggtcttgc ccgagccctg
     2821 caggccgacc atcatgatca ccaccggcgg ggcggcgttg agatccagcc ccggcacatc
     2881 ctcgccgccg agcatgtcga ccagcgcgtc gttgacgatc ttgacgacct gctggcccgg
     2941 cgtgaccgac ttcagcaccg actggccaac ggccttctcg gtcacctcgt cgatgaagcg
     3001 gcggaccacc ggcagcgcga cgtcggcttc aagcagcgcg attcgcactt cgcgcatggc
     3061 atcgcgaacg tcctgttcat tcagcgcacc acgaccgcgc agcctgtcaa agacagtgcc
     3121 aagccggtct gaaagggcgt caaacattcg caattcacct caaaacgcga aaaaagccgg
     3181 tgggcgaaac ctcgccgacc agcttgccgc taaagcactc aaaactctta tgaatggtgg
     3241 agcctagcgg gatcgaaccg ctgacctcta caatgccatt gtagcgctct cccagctgag
     3301 ctaaggcccc gtaccattca tcccgacaga ccctgggagg cccgtcgccc ctcgcttcat
     3361 ccgcgagagg ccgccccatt agtggcggcc ttcgcgcttg gcaaggggtt aattgtcgtc
     3421 cgggctttcg ccgtcgtcca ggcccacgcc caggtcgtcg tcgccaccca gatcgacgtc
     3481 gttgtccggc gaatcgccgt cctcgtcgat gtccagatcg gtgtcggcca gatcgacgtc
     3541 ggccacttcc tcgacctttt ccttctggat ttcttcatag ggaatcggct gcttcgactt
     3601 cagcaccggc tccggatgcc aggcatagcc gcattcgatg caggtgaccg ggtcgtcctt
     3661 gcccagatcg taaaagcggg tgccgcattt cgggcaaccg tgcttggtgc cccattcagc
     3721 cttgaccata ataaccctcg ctggcgaggc ccgtccggcc ccgcgaaaac tgctaaatac
     3781 gtattgggag gcagatgcgc cagtcccagc gcgggcgcgc cttgccatag cagggcggcg
     3841 ctgtcaaaag ccgcgcgcgt ttcttggcat ttcgatcgaa agttcccgct tgtccgcccc
     3901 cgcccacacc gacccgcgcc cccgccgctt tatggccact ggcccgctga agggcatgat
     3961 ccgcgtgccc ggcgacaagt cgatcagcca ccgctcgatc atgctgggcg cgctggcggt
     4021 gggcgaaacc cgcgtcaccg gcctgctgga gggcgaggac gttctggcca ccgccgcggc
     4081 gatgcgggcg atgggcgctt cggttgagcg cgtgggcgaa ggcgaatggt cggtccacgg
     4141 cgttggcgtc ggcgcgctgc tgcagcccga ggccccgctc gacatgggca attccggcac
     4201 ctccacccgc ctgctgatgg ggctggtcgc cagccacgcc atcgagaccc ggttcttcgg
     4261 cgatgccagc ctgtcaaagc ggccgatggg ccgggtgatc gatccgctga gccagatggg
     4321 cgccgcgttc gaagcttccg agggcggccg cctgcccctg accgtgcggg gcgccaaccc
     4381 cgccgtgccg atccgctacc gcctgccggt ggccagcgcc caggtcaaaa gcgcgatcct
     4441 gctcgccggg ctcaacacgc cgggcatcac cacggtgatc gaacccgtcc ccacccgcga
     4501 ccattccgag cggatgctgc gcggttttgg cgcggagctg gaggttgaaa ccgattcggc
     4561 cggcgtgcgc accatccgga tccgcggcga ggccgagctc aagccgcaga ccatcgaagt
     4621 ccccggcgat cccagctctg ccgccttctt cctggtcgcc gcgctggtgg tgccgggcag
     4681 cgagctgacc atccagaacg tcggcctcaa cccgacccgc gccgggatta tcgaagtgct
     4741 gcgccagatg ggtggcgaca tcaccgagct gaaccgccgc gacgtgggcg gcgaaccggt
     4801 ggccgacttg ctggtccggc attcggcgct gaagggcatc gcggtcgatc cggccgtggt
     4861 gccgagcatg gtcgatgaat tcccggtcct gttcgtcgcc gccgcgctgg ccgaaggcac
     4921 caccgtcaca accgggctcg acgaactgcg ggtcaaggaa agcgaccgga tcaccgtgat
     4981 ggccgaggcc ctgaccttgg ctggcgcgcg ggtgaccgaa accgaggacg ggctgaccat
     5041 ccacggcacc ggcggcgagc cgctgcgcgg cagcgccaat tcacgggtca agacccagct
     5101 cgaccaccgc atcgccatga gcatggccgt ggccggcctc gccagccgcg acggggtgga
     5161 agtggacgac acccgcccga tcgcgacgag ctttccggtg ttcgaggacc tgctggccga
     5221 ggtgacgcaa tgatccggct gcttgccgtt tccgccctgc tcctgacggc cgccgcgccc
     5281 gggctgccct acccttcgta tggtgccagc gcgttgcaac gcaaagccga cctcagcatt
     5341 tcggccattc cgatcgatac gcccttggcc aaggtcaaag gccgactaac aaaggtaccg
     5401 ggttcctgcg aagcggatgg caactgcgag tggcgcgaca aggccggggt ccggcactat
     5461 ttctggggcg acgtgaagcc cagcccgctg gtgatcaaat ttgtccatgc caaggatttt
     5521 cgcggccgcc cgatccgcgc gctcggcatt ggcatggccc ggtcaaagcc ggccgtcatc
     5581 aaggcggtca aggcgtttga ccccaggatc aagctggatt gcgatcgggc tcgggtttcg
     5641 ggcaacgttg ggccccagga atgttcgttc accaccaacc ccggctggat ccagatcggc
     5701 tttgatcgca acgaccaact gaccatgatc cggttcgacg gataccagtt cacatgatca
     5761 tcgccgtcga tggccccacc gccagtggca agggcaccat tgccaaggcg ctcgccgcgc
     5821 attttggctt gccgcatctt gataccgggc tgctctaccg cgcggtcggg cggcagtgcg
     5881 ctctgaacgg cggcgatgcc gacaatcccg gtgatgccct gtccgcctgc cagttccccg
     5941 acagcctgct ggacgatccc gagctgcgca gcgaggccac cggcgggctg gccagccggg
     6001 tctcggtcca ccccgccgtg cgccaggccc tgttcgagcg gcaacgcgcc ttcgccaccc
     6061 agcccggcgg cgcggtgctg gacgggcgcg acatcggcac ggtgatcgcg cccgaagccg
     6121 aggtgaagct gttcgtcgtc gcctctgtgc ccgcccgggc cgagcggcgc ttcaaggaaa
     6181 tgcaggccca gggccgcagc gtcagccttg ccgagatcgc cgcagacttg gaggcgcgcg
     6241 acgcgcgcga ccgcagccgc gtcgaagccc cgctggtcgc cgcgcccgat gccgtgctgc
     6301 tcgatacgtc cgatctgggg cgggacgagg cgatcgccgc cgcgatcgcg ctggccggtg
     6361 caaaatgccc gaaggcctga gcgcgctgct gctcggctgg gggctcgact gggcctggtt
     6421 caaggatgcc ctcgaacggg cgctgggggt cagcagcgac gccctgcacg tgatcgtggg
     6481 ggtgacgctg caactggctc tggtcgcgct gctgcgcacc acgctggccc ggattgcgcc
     6541 ctgggccctg gtcctggcgc tcaccctgct caacgaatgg aacgatttcc gggtcgaacg
     6601 ctggggcgat accgcgctgc aatggggcga agcggccaag gatgtcgggc tgaccatgct
     6661 gctgcccacg gtcctgctgc tgctggcccg ctggcggccg gggcttttcg ggctgcgcta
     6721 gaaccgggac agacgcttac gccggttgcc gggcccgggc cgggggtgat agggtcagcg
     6781 ccatgatcaa actcgctttc gccgccgccg ccgtgatcct gatcgccgcc tgcgccaccg
     6841 ttccggccaa gccgcgggtc gcactgaccg agcaggaagc gcgcggctat gcctttgcca
     6901 aaaacaattg cgcggcctgc cacggggtgg tcgccaacgc ctccagcccc aacccggaag
     6961 cgccgccgtt cgaagcggtg gtcaacacca aggggctgac cgcgcgaacg ctggaaacct
     7021 tcctgcgcga ttcgcacaat tttccgggcg cgatggcctt tgcggtggag ccggccaagg
     7081 tcgacgacat caccgcctat atgctcaccc tgcgcaagcc gggctacaag ccggggatct
     7141 gaagacgagg gcttgccgcc gcctgcggga tgatccccac cgccagctct gcccagatca
     7201 gcaacagcat cacgaccagc gcgagcgcgg ccgcgattcg caagtgccgg gacagcggcg
     7261 cgcgggccgc cagttccagc cccagcccgg tcaggcccag cagcagcgcc gcggccagga
     7321 aatcacccgg cccccagttc acctcgcgcg agaattgcat ggccacggcc ggcagggcca
     7381 gcacgcccgc gatgccgccc cacagcgcca ggcgccagga cgggggaaga ccagggcggt
     7441 ggatcggggc gtttcgggtc atgcaatcct ccgcgaggga acgcccatgg tctaggccgc
     7501 gttccggccc gccgcacgtg gagatcgtgt gaatttgccg tgcaggctcg ggcggaaggg
     7561 gcttgacggc gcgcacagat gtttcataac aggttatata acgagatata ggaattctcg
     7621 gatgcgcgac gtcattgccg aaatgggacc gtccttcctc gcctcgcggc tgaagcggtt
     7681 ggccgaacgc atgcaagccg gcgcggccag cgtgattgcc gatgccggcc tgccgttgca
     7741 gcctgggcac atggccgtgc tggccgcgct gcgcaccggg cccaagaccg tcgggcaact
     7801 ggccgatgtc acggggacca gccagccggg catcactcgc acggtcaagc agctggtcga
     7861 actcgggatc gtcggcaacg ccagccgcga agaccagcgc agccgcgccg tgatgctgac
     7921 cgccgcgggg gaagaggcgg cgcggatcgc gggaaccgat atctggccgc gcgtgggcca
     7981 ggcggccgaa cagctgctgg ccggctgcgg cagcgatttc ctgcacgaag tggcgcggat
     8041 cgaggcggcc ttgaacgaag cctcgatcgc ggcgcgcgcg gccgcgctcg aaccgggcgg
     8101 actgcgcctg cgcgagttcg acgattcgct ggccggtgcg ttccacgaca tcaacgcgga
     8161 gtggatcagc accatgttcg cgctggaaca gaccgaccgg gacgtgttgg agcaaccgcg
     8221 ggcccggatc atcgatggcg gcggcacgat cctgttcgtc gaggcgccgg gtctcggcat
     8281 cgtcggcacc tgcgcgctgc aaaagaccgg gccacgcagt ttcgagctga ccaagatggg
     8341 ggttcgcgaa gtcgcgcgcg gcctgaaggc cggagaattc ctgctggaaa agacgatcgc
     8401 ccgggcccgc gaaatgggcg ccgatccgct ttacctgctg accaaccgca agtgcgccgc
     8461 ggcgatccat ctttacgaga agctgggctt tgcccacgat gcagggatca tggccgatta
     8521 cggtgcgcgc tatcagcgct gcgacgtggc gatgcgctat accggctgag cgcaaaacgc
     8581 gccattttcc ttgcatttgc cgccccccgc gcataagggc cgccggtccg atggagcctt
     8641 ggcttcaaag ggcgcggtgg acggcatgcg gcccccatcg cagctcggcc ccttttggcc
     8701 cgtgtgcggc aatcccgctg ccacggagaa agaccggcgg aaacaaccgc ctggccggaa
     8761 acatcgatac gaggaaactc acctatggcg tctaccgcca accccacccg cgatgaattc
     8821 gcgg
//
LOCUS       DASUDV010000409         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_985805,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000409 DASUDV010000000
VERSION     DASUDV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985805"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFV30_08885"
     CDS             <1..724
                     /locus_tag="VFV30_08885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4848247.1"
                     /translation="LRGQWVLGVLEPDCCDIDVAALHQLWLGQARRGGAELACRSRLV
                     AAQRSASGWQLQLEGGRSLACDVLINAAGAWADPVAELAGARALGVQPYRRTMVQLAM
                     TSPVPRDLPLVLGLDGSFYFRPEGEKLWLSPHDEIPSGACDAAPEEIDVATAIARFED
                     VADWPIARLEHKWAGLRSFAPDRLPVYGFDPRVPGFFWFAGQGGFGIQTAPAAAELAL
                     RLICGETPGTVDPAPYLPDRFS"
     gene            777..959
                     /locus_tag="VFV30_08890"
     CDS             777..959
                     /locus_tag="VFV30_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEX4848248.1"
                     /translation="MAHRFEIRKNKAGEFVAYFCYNSETIFWTEGYSSKASAKNAIES
                     IKKNGPGADTVDTTTD"
     gene            complement(963..1397)
                     /gene="rnhA"
                     /locus_tag="VFV30_08895"
     CDS             complement(963..1397)
                     /gene="rnhA"
                     /locus_tag="VFV30_08895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX4848249.1"
                     /translation="MKKVEVFTDGACKGNPGPGGWGALLRMGVHEKELSGSEPLTTNN
                     RMELTAVIRALEALTQPCQVQLHTDSRYVIDGITKWIYGWQKNGWKNAAKKPVLNADL
                     WQELLGAVRRHRIEWIWVKGHDGHPENERVDRLASDAALAAS"
     gene            complement(1394..>2282)
                     /gene="thrB"
                     /locus_tag="VFV30_08900"
     CDS             complement(1394..>2282)
                     /gene="thrB"
                     /locus_tag="VFV30_08900"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX4848250.1"
                     /translation="IETTGKDGQGARFILTMYEFRIELEDLPFFLSLLDHLAARGCPV
                     PRTIHDRSGALFRMVGDPGSPAGAGKAVALIEFLPGVSVSQPSPAQARAVGGALAQLH
                     LAAADFPGSRANTMGLAEWQRLAGACGPEGLARIDPALPGLVERELEFLARNWPADLP
                     HSVIHADLFTDNVLMLGDRVSALIDFYFACTDITAYDVAVTHAEWCFDPTGRRFDPAL
                     SAALLAGYEAVRPLSVAERAALPVLARGAAMRFVMSRAWDWLNTPRDALVTPKDPMAF
                     ARRLEFYADPTNAALFGAR"
BASE COUNT          364 a          758 c          772 g          388 t
ORIGIN      
        1 actgcgcggg caatgggtgc tcggcgtgct tgagcccgat tgctgcgata tcgacgtggc
       61 agcgctgcac cagctgtggc tgggccaggc ccggcgcggc ggggcggagc tggcctgccg
      121 ttcgcggctg gtcgcggcgc agcggtccgc ttcgggctgg cagcttcagc ttgagggtgg
      181 ccgcagcctt gcctgcgacg tgctgatcaa tgccgccggg gcctgggccg atccagtggc
      241 ggaactggcc ggagcgcgcg ccctgggcgt ccagccctat cgccgcacca tggtgcagct
      301 ggccatgacc agcccggtgc cgcgcgatct gccgctggtg ctgggccttg atggttcgtt
      361 ctatttccgg cccgaaggcg aaaagctttg gctgagcccg cacgacgaga ttcccagtgg
      421 agcctgcgat gccgcgcccg aggagatcga tgtcgccacg gcgatcgcgc gtttcgagga
      481 tgtggccgac tggccgattg ccaggcttga gcacaaatgg gcgggcttgc gctcgttcgc
      541 gcccgaccgg cttccggttt acgggttcga cccgcgggtg ccgggcttct tctggtttgc
      601 cgggcagggc ggcttcggca tccagaccgc accggccgcg gccgagctgg ccttgcggct
      661 gatttgcggt gaaacgcccg gtacggtcga tccggcgccc tatttgccgg accgatttag
      721 ctagcgaatc gcttgtttca gagtaacttg cgcactccac aagttggagg gacgcaatgg
      781 ctcaccgttt tgaaatccgc aagaacaagg ccggcgaatt tgtcgcctac ttctgctaca
      841 acagcgaaac gatcttctgg accgaaggct attcaagcaa ggccagcgcc aagaacgcga
      901 tcgaatcgat caagaagaac ggccccggcg ccgatacggt cgataccacg accgattgat
      961 cgtcaggacg cggccagcgc tgcgtcgctt gccagacggt ccacccgctc gttttccggg
     1021 tggccgtcgt ggcccttgac ccagatccac tcgatccggt ggcgccgcac cgcgcccagc
     1081 agttcctgcc acagatcagc gttgagcacg ggcttcttgg cggcgttctt ccagccgttc
     1141 ttctgccagc cgtagatcca cttggtgatc ccgtcgatca catagcggct gtcggtgtga
     1201 agctgcacct ggcacggctg ggtcagcgcc tcgagcgcgc ggatcaccgc ggtcagctcc
     1261 atgcggttgt tggtggtcag cggctcgctg cccgacaatt ccttttcgtg aacccccatc
     1321 cgcaacagcg cgccccagcc gcccgggccc ggattgccct tgcaggcacc gtcggtgaac
     1381 acctcgacct ttttcatcgg gcgccgaaca gcgcggcatt ggtcggatcg gcatagaatt
     1441 ccagccgccg ggcgaaggcc atcggatcct tgggggtgac cagcgcatcg cgcggggtgt
     1501 tgagccagtc ccaggcccgg ctcatcacga accgcatggc cgccccgcgc gccagcacgg
     1561 gcagtgccgc gcgttccgcc acgctcagcg ggcggaccgc ttcgtacccg gcgagcaagg
     1621 ccgcgctcag cgccggatcg aagcggcggc cggtggggtc aaagcaccat tcggcgtggg
     1681 tcaccgcaac atcgtaggcg gtgatgtcgg tgcaggcgaa atagaagtcg atgagcgccg
     1741 aaaccctgtc gcccagcatc agcacgttgt cggtgaacag gtcggcgtgg attaccgaat
     1801 gcggcagatc ggcgggccag ttgcgggcca ggaattccag ctcgcgctcg accaggcccg
     1861 gcagggcggg atcgatcctg gcaagaccct ccggcccgca ggcacccgcc agccgctgcc
     1921 attcggccag acccatggtg ttggcccggc tgcccggaaa atccgctgcg gcaaggtgca
     1981 actgggccag cgctccgccc accgcgcggg cctgggccgg gctgggctgg ctgaccgata
     2041 cgccgggcag gaactcgatc agggccacgg ccttgcctgc cccggccggc gagccggggt
     2101 cgcccaccat ccggaacagc gcgcccgagc gatcgtggat cgtgcgcggc acggggcagc
     2161 ccctggcggc cagatgatcg agcagcgaaa ggaagaaggg caggtcttcc agctcgatcc
     2221 ggaattcgta catcgtcagg atgaagcgcg cgccctgacc atccttgccg gtggtttcga
     2281 tc
//
LOCUS       DASUDV010000410         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_323209,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000410 DASUDV010000000
VERSION     DASUDV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323209"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFV30_08905"
     CDS             <1..723
                     /locus_tag="VFV30_08905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX4848251.1"
                     /translation="KANPMPNFKGTRFTNAHETLIWCSMGEKARYTFNYRAMKTLNDE
                     LQMRSDWVLPICSGQERLKKGGTKVHPTQKPEALLYRVMLATTNKGDVVLDPFFGTGT
                     TGAVAKRLGREWIGCEREDIYREAALERIELALPLDESALTTMQSPKAAPKVAFGTLI
                     ETGWIAAGAVVSDKKRRFKATVRADGSLACGTDSGSIHGLGAKVQGAPSCNGWTFWHI
                     EHEGELKPIDAIRQLYLLATED"
     gene            720..1814
                     /gene="folP"
                     /locus_tag="VFV30_08910"
     CDS             720..1814
                     /gene="folP"
                     /locus_tag="VFV30_08910"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX4848252.1"
                     /translation="MTHYLRPIALCDSPQSEAGEALRLAGGLAYASRFALIEREGGRI
                     VSRQRLARHEIEVILPSLPEAVQQQWQDLQRSHAPLECGNRTVRLDQPQVVGILNVTP
                     DSFSDGGQFMDDPEVANAHAAAMVEAGAALIDVGGESTRPGAAAVWEGDELKRVIPAI
                     ERLAAMGAAISVDTRRPAVMEAALAAGAHVINDVSALRHDPRSLEFAARSGAPVILMH
                     APGEGEDLHGGGGYGDVVLDVFDWLRARRDAAIEAGIARDKIVLDPGIGFGKSIADNL
                     ALLNALPLFHALGQPLMLGASRKRMIGALSNEAPAHQRLGGSLALAMKGLEAGVQLLR
                     VHDVAETVQAARVWRGLRDAALTDFTQLAD"
     gene            complement(1799..>2641)
                     /locus_tag="VFV30_08915"
     CDS             complement(1799..>2641)
                     /locus_tag="VFV30_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEX4848253.1"
                     /translation="FTLESSGDLKNWEHLAEKVLFSPSAGQQPLGGAQMALAGSDLKQ
                     RYLRLSWQANAGVKLSGAFIDTARTAPPAALPLATKGARLDDAHNLRLAVATPAPLAG
                     IELGGTKADGLLPLRLYGRDAGEQPWQALGAATLRGDGRPARFDLDSAAFRQYRIEAD
                     QRSAGFSQAPAVFLQLQPVTLIAAFNGEAPYRLAVGNAGAEGKYFAPADLTDAKTAPA
                     DLPLAKVTAAAAPVIDLAAGAADTPFTPRKLALWGALLLGTALLAFGAIRMLRANAPD
                     QSAS"
BASE COUNT          457 a          830 c          923 g          431 t
ORIGIN      
        1 aaggccaacc cgatgcccaa tttcaagggc acgcgcttta ccaatgcgca cgaaacgctg
       61 atctggtgtt cgatgggcga gaaggccagg tacaccttca attatcgcgc gatgaagacc
      121 ttgaacgacg aacttcagat gcggtctgac tgggtcctgc cgatctgcag cgggcaggag
      181 cggctgaaga agggcggcac caaggtacat cccacgcaaa agcccgaagc gttgctctac
      241 cgggtcatgc tggcgaccac caacaagggc gatgtggtgc ttgatccgtt cttcggcacc
      301 ggcacgactg gcgccgtggc caagcgcctg gggcgcgagt ggatcggctg cgagcgcgaa
      361 gacatctacc gcgaggccgc gcttgagcgg atcgaactgg cgctgccgct ggatgaaagc
      421 gcgctgacca ccatgcagag ccccaaggcc gcgcccaaag tggctttcgg cacgctgatc
      481 gaaaccggct ggatcgccgc cggggccgtg gtcagcgaca agaagcgccg cttcaaggca
      541 accgtcaggg ctgatggctc gctcgcctgc ggaaccgata gcggatcgat ccatggcctg
      601 ggcgccaagg tccagggcgc gcccagctgc aacggctgga ccttctggca catcgaacac
      661 gaaggcgaac tcaagccgat cgacgcgatc cgccagctct atctgctggc gaccgaagat
      721 tgacccacta cctgcgcccg attgcgctgt gtgacagtcc ccagagcgaa gcgggcgaag
      781 ccctgcggct ggcgggcggg ctggcctatg ccagccgctt tgccctgatc gagcgcgaag
      841 gcggcaggat cgtttcgcgc cagcgtctgg ctcgccacga gatcgaggtg atcctgccca
      901 gcttgcccga agcggtgcag cagcaatggc aggatttgca gcgcagccat gccccgcttg
      961 aatgcggcaa tcgcacggtc cggctcgatc agccccaggt ggtggggatt ctcaatgtca
     1021 cgcccgacag tttttccgat ggcggccagt tcatggacga tcccgaggtg gccaatgccc
     1081 acgccgcggc gatggtcgag gccggggccg cgctgatcga cgtcggcggc gaaagcaccc
     1141 ggcccggtgc ggcggcggtg tgggaaggcg atgaactcaa gcgggtgatc cccgcgatcg
     1201 aacggctggc ggcgatgggt gcggcgatca gcgtcgacac ccgccgcccg gcggtgatgg
     1261 aagccgcgct ggcggccggc gcgcacgtca tcaacgatgt ttcggccttg cgccacgatc
     1321 cgcgcagcct ggaattcgcg gcgcgttcgg gcgcgccggt gatcctgatg cacgcgccgg
     1381 gcgagggcga ggacctgcac ggtggcggcg gctatggcga tgtggtcctc gatgtgttcg
     1441 actggctgcg cgcgcggcgc gatgccgcga tcgaggccgg gattgcgcgc gacaagatcg
     1501 tgctcgatcc gggcatcggt tttggcaagt cgattgctga taacctggcg ctgctcaacg
     1561 ccttgccgct gtttcacgcg ctgggccagc cgctgatgct gggtgccagc cgcaagcgga
     1621 tgattggcgc gctatcgaac gaggccccgg cccaccagcg gctgggcggc agcctggccc
     1681 tggcgatgaa gggacttgaa gccggggtgc aactgcttcg ggtccacgat gtggccgaaa
     1741 cggtccaggc ggcacgggtc tggcggggcc tgcgcgatgc ggcgctgacc gattttactc
     1801 agctggcgga ttgatcgggc gcgttcgccc gcaacatccg gatcgcccca aaggccaata
     1861 gcgcggtccc cagcagcagc gcgccccaca gcgccagctt gcgcggggtg aagggagtgt
     1921 cggcggcgcc tgcagcaaga tcgatcacag gtgcagccgc cgcggtcact ttggccaatg
     1981 gcagatcggc gggcgcagtt ttggcatccg tcagatcggc aggggcgaag tatttgcctt
     2041 cggctccggc attgccgacg gcgaggcgat agggtgcctc tccattgaac gctgcgatca
     2101 gtgttaccgg ctgcaattga aggaaaacgg cgggtgcctg cgagaagccg gcgctgcgct
     2161 ggtctgcctc gatccggtat tggcggaatg ccgcactgtc gaggtcaaag cgcgccggac
     2221 ggccatcgcc acgcagcgtc gccgcgccaa gcgcctgcca tggctgttcg cctgcgtcgc
     2281 ggccatagag ccgcagagga agcagcccat cggccttggt gccgccaagc tcgattccgg
     2341 caagcggtgc aggcgtggca acggccagtc gcaagttgtg agcatcgtcg agacgcgcgc
     2401 ctttggttgc cagcggcaag gcagcgggtg gggcggtcct ggcagtatcg atgaaggcgc
     2461 cgctcagttt caccccggca ttggcctgcc agcttagccg caggtagcgc tgcttgaggt
     2521 cgctgccggc aagcgccatc tgcgcgccac ccagtggctg ctggcctgca gaggggctga
     2581 acagcacctt ttcggccagg tgctcccagt tcttgagatc gccgctcgat tcgagcgtaa
     2641 a
//
LOCUS       DASUDV010000411        16661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_168817,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000411 DASUDV010000000
VERSION     DASUDV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168817"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFV30_08920"
     CDS             <1..675
                     /locus_tag="VFV30_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848254.1"
                     /translation="YVHRIGRTARAGADGVALSMVAPDEKAYLKDIERLTGVTLARAP
                     LPENFQQEAARLPAPARRSPAEMEQDARRDERDARGLGGGRGGARGGRGPRPNSNRAN
                     GGQSRDGQSRDQYIRNERFADSRKATAPRGPVFNPLGGEDREARAHAPRDDRAPRRDD
                     RRDDRPRGERTERPVKLHRDAVRPAGGHAPRADRPRADGARAEGQQRRKPAGKKPNGS
                     GQRRWG"
     gene            complement(874..4392)
                     /locus_tag="VFV30_08925"
     CDS             complement(874..4392)
                     /locus_tag="VFV30_08925"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX4848255.1"
                     /translation="MTLRNDATRLRVPQRDKRMRRGVVARQGQVLLDADLNQQGDLLL
                     GRVEAEARALMGSPGKLVYPAGTTGFAIGAAGTPLAVTIGAGEGYLDGWRLENPDGCN
                     LASQPHPFTPAPNAPVLIALRAAVRHIDPVEERAMADVALGDAQAPGRALNDWQVLPV
                     PLTTTNPLTDPAWTAIKAASTGKLAFRRQQPSGPTDICSLTPQGGYSRHENLLYRVEV
                     HGGVAAETHADGPRYGLNGLKLKFSRRNASIMARVIGISGNKITVAPPPFDERTWFAP
                     GSYAELVSIHDDANPSGLPAAERLFKVSTVDGADVFLQEGGGATVAATGAAPPATPAE
                     EANWFLRCWDTFPVSGGTSSGIATVALAANGTDSLRIDLGDGLAVELTGGAAARFRRG
                     DYWTCAVRADGTVAWADGALANVAEAPHGPEIRYAPLAHVTASGVTDLRIPLATLTDR
                     TLLYRGGDGQMAPGTASGLARLPAMLRVAVMRGETPVAGAPVRWRILAPTGTGGRLEY
                     LAQTGANLVVPTNDQGISEVAWSIDAAQPTALHQVSADLDDGPQPGTPPLLFSAGFDR
                     EGSHGGCSTYVIPEGSDWVTLLEQMDFSQDIAVCFQRGEYRTSRPVVLSGTRSIKISG
                     AGDGTLLLARHGETVLRLEGFASAVVERLRIETRDSLKLTNRKANGLSGRGGALTLQG
                     CLYAEVRNTALRCGGALATERTALTSAGTEERPVSVARVIDNRIIAGFLQDGVLVVNC
                     RDSTVAGNQLVAGKRSAKLGAKAVSDAKAWKLAVVRALVSRIKFRQRPGTNPELRGFR
                     LGRVTVEFASAAPQADWDALAVLRPWEGGDNPTEAEALTYLEALVDTAMRLDAEAPEA
                     VRALLKESERRGARFDPAGKRTMALAAMLPSGVKVSDARRPRPDRGALKRLTAIENGL
                     RLGAMEVTFESPIPHAVWAQLYNDDTVYRPGRPRHATDLARQVRAMAMLLASDATVRN
                     RFSAVAGWYGIWAEPAEAAARQAITCGGAVLENVAVTGNRVRGYACGVHVGVSARVEK
                     GGNGGKPRDGTRTALNARIADNDLALQLPSGLHYYPAGLFLGNARTGRIDRNVLSRSD
                     GMGDNGGWRHGIRVWGLIGNYLMIAENRIAGPRIGIKVRPTNMAGSQPDPQFLWVARD
                     NLVDWVPVGNVLHAPALVQNRDNRPV"
     gene            complement(4389..6821)
                     /locus_tag="VFV30_08930"
     CDS             complement(4389..6821)
                     /locus_tag="VFV30_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848256.1"
                     /translation="MTKPVSEARIAAETARLMGLLPAHIRAVDAESGHALEALMGVLA
                     RGVAEIDGEIDALYDGLFAETASPASLDEIAALIGAIPLRPLPEGAGHNSRAYVANTL
                     RYRRGKGTARVLEELAADVGGFGAVAVEYFMRLARTAHLADLRPERPATARLVPGETA
                     ARAGSGFDRLPRLVDVRSIARAGGAHHVPHVGVHVVRPLAPAYPAPSDLAALTETEVA
                     GVPIAAPWKPGGTAQAGYFQLAAVPGAPLRLFNPDRRSQDGADRMVEQDLADRLTRLP
                     LYQELEELRHAALEQREAQLTPIRWFNPQGEPFAVLLGRRQGGKLLFDRVPPERICIA
                     NLEDPPVPAGARPPAQRAHRWVAGSPSGATDKTGNSPVTCAFDPVTGRLILADPLPGL
                     PVEAVRVAHAYGLGREIGAGPFDRNDPDVPFAILAAPGIQTDVWLVGPRDLPLPAGVN
                     QAASLADAVAAWNGLAGPNRRGYIVLTSCDRSGGSAPITVKVPGGSELHILSSRFEDP
                     QVRPGVDPDPALRGYLVRKGRSFVVEVPLIADTQISPALGGRLVLDGLSLTAGLELRA
                     GAVSDLLIRFCSLRAPGGAALSTTAPFRGAAVTIDRSLTGPLRLHASGATATGTLAVR
                     DSVVAADGAAGRAIDSFQLETRLDNVTVLGPVRCKELEATNALLRDPVTVTRTQAGCV
                     RYSWIAPGSSVPRTFRCQPQMALAAAAAAKGAPLGPSEREAIELGLAPQLLDLSLDEP
                     TVAMLAAHSPRAIISGGEGESEMGAFSPGALGLRQANMERLFEDYIPFGNEAGLIDDT
                     RSSAVAFRRNRP"
     gene            complement(6818..9505)
                     /locus_tag="VFV30_08935"
     CDS             complement(6818..9505)
                     /locus_tag="VFV30_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX4848257.1"
                     /translation="MSEPCGCGTCASCGGPGGVPQRPVGDPLRFRHAAIRQRMLDSIA
                     AARVGTARPLDRLGVRSSDDPAIALIDAQAASLHVLAWTGARLADDGTILRSEDRDAL
                     VDLGRLIGYEPRPALSAATTLAFTLETLPGAPAKVRVAKGTKVASVPGQDEKPQVFET
                     DAEFEAVGAWNAIRPVCPLPDITGSTRTLCIAGVESAAREGDFVAVPRLPQGGGADDW
                     LIGRIAGIERVSLPPDPAFLRFTLSDCWHVETTAALAAARAGEVAILARRAAAFGATA
                     PDARAMTDDVRAGLISDDPETGATQWDNFEVGAPGSSGGKTIDLDAAYPEAFAGRLAL
                     FTRANKRPPFDYSKLKFVRTGETMEENDALARAHHEAAEERRRDMEEYTAGQVTDPAL
                     TEITKAVELARTDFLLSAKVSRITLDQIDLSDDGKAASGKSASLRYLVRELAVFLETE
                     RQTLYRPPRDIALPSESDRLVVQGKIEMAPGRRVILSGEKWMGQAGVTGPQQAAVAIV
                     RTAQVNADGTTTLLFEDPVAARFRSTRLAVLGNCVTASHGETPAQGEEIIGSGKAGAR
                     DQRFALKGKPLAYIPAETPRGYAPAVEVRVGGRAWKEAPHLSGLSAADRAYTVRPGRD
                     GTAVIQLAGQLPSGLNNVTALYRTGGGKAGNLAAGRITMAMVPVQGVIGITNPLPADG
                     GSDAETIEDIRRAAPLSVRTLDKVVSLADYEAFAAAYRGVGKALATLLYAGMRQEVCL
                     TLADSQMQSPAEGSSVVEGLRRELKRVQVPGRTVRIAGFTAIHPAITLALACDPAFRR
                     KDVQEAVRTLLAKRFGPAARPFARALHRSEVLAAAQGVDGVIAATLTVFALPSGMPAE
                     DEGRLLCPGPILDPGTGDLIPAGLLSIDPAQVLFEEMAA"
     gene            complement(9502..11985)
                     /locus_tag="VFV30_08940"
     CDS             complement(9502..11985)
                     /locus_tag="VFV30_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848258.1"
                     /translation="MNTDRYLCCDEQRRTLLAAPGVPFTGIDYVEVRTGTSLADPVFI
                     DIYLVKPMPLAAAALTTANFRLTGGTRFPAPTRFKLLTPLPNPVDKYVLEIEPGQPTD
                     FSAYVLRIVAAADSLAPPAFLDGRLAAIDFSFKVGCAGDFDCADPCDPPPDALGPDPE
                     FDYRVRDYQGFRRLMLDRMAALVPGFREDNPVDFTTTLVEALAYRADQLSYKLDWVGT
                     EAFLSTARSRTSLSRLARLVDYTPGEATSARVFVRIDPQDAASDGQVIASATPLLVRR
                     EGAGTAIPAGDYARLGDTAGPVFETVAPLRVWRWRNAIALHTWSDETCTLPRGSRAAT
                     LVDASAASGGLAKGDFLLLEEIVSPTTGVAADARPANRHVVRLTKVTPVNDVLQPGLK
                     LVLAEWGEADALPFDLVIQARVAGGLGSSSMVTCARAAANLMLADHGRSLPPPAHLGL
                     TPSEAGALEPRLDPPAPGDICDWQPRLDRGGVARTRPFRLTDSDADASARALAAVDPA
                     TCLAAFELLDDFGTWQARPDLLKSSRFSREFVLETAIDGHLALRFGDGINGLCPDPDM
                     RISARGRFGSGIEGNIGIGALAHLVVPASQAGLSVTVGNPLPAAGGQAAEPASQVRIN
                     APIAFRRQERAVVAQDYADAAMRHPGVANAVAEPIWTGAWQTMLLYIDRTGGLELDAP
                     FRRDLLRHMERFRLIGFDIALHGAQTVALDLAFLVCAEPNALRSTVAAGIRAALRPGG
                     GDQRGFFHPDNFTFGSPLYLSQVVAAIMAVDGVQSVTPRRFQRLGFAPDDALARGVIT
                     PGPLEVLQLADDPSFPERGRLGLEMGGGR"
     gene            complement(11982..12356)
                     /locus_tag="VFV30_08945"
     CDS             complement(11982..12356)
                     /locus_tag="VFV30_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX4848259.1"
                     /translation="MTRLAFPFGPGADGRSRTVGYGDAAHVEQMLELLILTMVGERVM
                     RPDLGSPVRQMVFQAGNSPAALALEASLQATITQWLGQVLELQGLSVDFDEAEARLEV
                     VVAYEIRATKTGGRVAVRKDLT"
     gene            complement(12353..12700)
                     /locus_tag="VFV30_08950"
     CDS             complement(12353..12700)
                     /locus_tag="VFV30_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848260.1"
                     /translation="MPGPVLHSAAVATCPHGGSLSIVAASPRVTVSGMPVAVLTDQGM
                     VAGCPFTVPIPGPATKPQPCVTTKWMVGATRVTAGGVPVLINPCVATCFSAEQIPGGP
                     PVISSSQMRVVAT"
     gene            complement(12727..13242)
                     /locus_tag="VFV30_08955"
     CDS             complement(12727..13242)
                     /locus_tag="VFV30_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX4848261.1"
                     /translation="MTAGPRYFGKYRATVLNNLDAQSQGRIQVQLGDRYGPFPSTWAL
                     PSLQFAGKGMSGIVALPAIGSMVWVEFEAGDPDYPIWTGSFWPDPAGFPTQALAGATP
                     ATPNIHLQTTTGTSVTLSDNPAQQVFIKTVAGAMISVGASGITISNGQGASIMLSGPS
                     VIINGGALTIT"
     gene            complement(13239..14357)
                     /locus_tag="VFV30_08960"
     CDS             complement(13239..14357)
                     /locus_tag="VFV30_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848262.1"
                     /translation="MATLKGVTLTLLMGPVAVAPVPVAVIEALESAQVTSAVGQRSGF
                     QISFTYSKTSPIATTLLPAGFFDPMIRVILVATLNGVPKVLADGPIKRQDVVASSAPG
                     ENRLTITGEDVSGYMDLIDFTGFPFPAMPTFARVAALLAKYAMFGVIPVPVPEVFSTV
                     ELPIKSYAHQQGTDFEYLNKLAKEAGYEFYVTPGPAPGTNTAYWGPQLRVGLPQPALT
                     IDMDHASNIESMSFSADGNAAIMPYCYVKIGGFSVPVPVPNISLLAPPLAARPLIPTK
                     IRPVETERTEMPKAMALLLAGRAAAEPVSANGSIDLSRSGSPIDARSIIGVRGAGLAH
                     DGLYYVRSVTHTLGRGSWKQAFQLQRDGLISTVPAVPL"
     gene            complement(14344..14664)
                     /locus_tag="VFV30_08965"
     CDS             complement(14344..14664)
                     /locus_tag="VFV30_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848263.1"
                     /translation="MTDTFPPNSRYAGVPLRETTLPDGTTRRFVGRRIIPQTGRYQAL
                     DRHRAAGDERIDGLAAASYGDPELYWRICDAGGDADPGEAAGPAGRLILIPLPLEMGD
                     NGDT"
     gene            complement(14661..15317)
                     /locus_tag="VFV30_08970"
     CDS             complement(14661..15317)
                     /locus_tag="VFV30_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848264.1"
                     /translation="MTTFPGSPKTLRGGLVLMDAEGKTVQRTVAFQYNPDTVTRSLTP
                     RGAKIDAGDRLEGLRLVGPPVETIKIDIEFDATDRLEHPGQNKATVEDGIAPELAELE
                     AILSPDPAAIEAADALARTGTLEVLPLPSPMIVLVLGRNRLLPVRITEFSVVEEAHDT
                     RLNPIRAKIALGMRVLSIDDLAFGSKGAQLFMTAARRRDALSKRKPPSLQGLGLTGTP
                     "
     gene            complement(15314..>16661)
                     /locus_tag="VFV30_08975"
     CDS             complement(15314..>16661)
                     /locus_tag="VFV30_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848265.1"
                     /translation="GTIQPEVAIPGGAAADGVVSYVWGRGWAGEAKASGTPFKGATAD
                     IAFGFAETAGVLGFYGTGGISCKIRDADLTLKAGWKGAGVSYKGGVKIKKPLPLVDEV
                     DFNGSYGDGLLHLGGSGNLSVAGFTGRMQVNYDQKDGEDGTFSGSAHIQKKGMLGGKA
                     DADFKLNYSKEGKLSGSGILAYQVTPAFRPELGVHLLEDGRIKVKGSVTVTNFQVFKA
                     WPGPGKDRRSILKGGISIGIPIPPFPVVQATLSVTGELGVAFGVGPGLINGTLTAELF
                     PFEEDKKIKATLDGKFTVPAKAELYGRFGALLGVAIAGGLAGVEGGIYLKPAIGVKGE
                     GGIGVKAEYADGGFSFEANAFATGAMYGSLGIDFAAAVYLARNLLRYEWTHPLAKYGP
                     VQLGPTLDVQLGKIAYARDGTVTWPDPSQISITPKEFDPLQLVADVLKGSKMSPEP"
BASE COUNT         2456 a         5980 c         5783 g         2442 t
ORIGIN      
        1 tacgtccacc gcatcggccg caccgcgcgc gccggggcgg acggcgttgc cctgtcgatg
       61 gttgcgccgg acgagaaggc ctacctcaag gacatcgagc gcctgactgg ggtgaccctg
      121 gcccgtgcgc cgctgcccga gaacttccag caggaagccg cccgtctgcc cgctcccgcc
      181 cggcgcagcc cggcggaaat ggagcaggac gcccgccgcg acgaacgcga tgcgcgcggt
      241 ctgggcggag gccgtggcgg tgcccgcggc ggacgcggtc cgcgccccaa cagcaaccgg
      301 gccaacggcg ggcagagccg cgacggccag tcgcgcgatc agtacatccg caacgagcgc
      361 tttgccgaca gccgcaaggc caccgcgccg cgcggccccg tgttcaaccc gctgggcggc
      421 gaggaccgcg aggcccgcgc ccatgccccg cgcgatgacc gcgccccgcg ccgcgacgac
      481 cgccgggacg accgtccgcg cggcgagcgg accgagcgtc cggtcaagct gcaccgcgac
      541 gccgtgcgtc cggccggtgg ccacgccccc cgcgccgacc gcccgcgtgc cgacggtgcg
      601 cgcgccgagg gccagcagcg ccgcaagccc gccggcaaga agcccaacgg cagcggtcag
      661 cgccgctggg ggtaatcagc tggaaatgat gaggggggtc aacctattac ggtatccccc
      721 ccctgtcacg acccgactcg aacgatcaca taccgttccg cgcgaaactt acgcaccgcg
      781 cagttgtgcc tttaatcgcg acaatacctt taaataccac ggcccgcggg cggctccaag
      841 cggaatgccg gtcgcgggcc tcggtttgcc gcatcagacc ggccggttgt cgcggttctg
      901 gaccagcgcc ggggcgtgca ggacgttgcc caccggcacc cagtccacca ggttgtcgcg
      961 cgccacccac aggaactgcg gatcgggctg gctgcccgcc atgttggtcg gccgcacctt
     1021 gatcccgatc cgcggtccgg cgatgcggtt ttcggcgatc atcaggtaat tgccgatcag
     1081 gccccagacc cggatcccgt gccgccagcc gccgttgtcg cccatgccgt cagaccggga
     1141 aaggacgttg cggtcgatcc gtccggtccg ggcgttgccc aggaacagcc cggccgggta
     1201 gtagtgcagg cccgagggca gctgcagcgc caggtcgttg tcggcaatcc gggcgttaag
     1261 cgcggtccgc gtgccgtccc gcggcttgcc cccgtttccg cccttctcca cccgcgcgct
     1321 gacgccgaca tggaccccgc aggcatagcc gcgcacccgg ttgccggtga ccgcgacatt
     1381 ctccagcacg gccccgccgc aagtgatcgc ctgccgcgcc gccgcctcgg ccggctccgc
     1441 ccagatcccg taccagcccg cgaccgcgga gaaccggttg cgcactgtgg cgtcgctggc
     1501 cagcagcatg gccatcgcgc ggacctgcct cgccagatcg gtggcatggc gcggacggcc
     1561 ggggcgatag accgtgtcgt cgttgtagag ctgggcccag accgcatggg gaatcggcga
     1621 ttcgaaggtc acttccatcg ccccgagccg caggccgttc tcgatcgcgg tcagccgctt
     1681 cagcgcaccc cggtccggcc gtgggcggcg ggcgtcgctc accttcaccc cgctcggcaa
     1741 catcgcggcc agagccatgg tgcgtttccc ggccggatcg aaccgcgccc cgcgccgttc
     1801 gctttccttg agcagcgcgc gcaccgcttc cggagcctcg gcatccagcc tcatggccgt
     1861 atcgaccagc gcttcgagat aggtcagggc ctcggcctcg gtcggattgt cgccgccctc
     1921 ccacgggcgc aggacggcca gggcatccca gtccgcctgc ggcgccgccg aggcgaattc
     1981 caccgtgacc cgccccaacc ggaacccccg cagttcgggg ttggtccccg ggcgctggcg
     2041 gaacttgatc cggctgacca gcgcgcgcac cacggccagc ttccaggcct tggcgtctga
     2101 caccgccttg gcgcccagct tggcgctgcg cttgccggcg accagctggt tcccagcaac
     2161 ggtcgagtcg cggcagttca ccaccagcac gccgtcctgc aggaagccgg cgatgatccg
     2221 gttgtcgatc acccgggcga ccgagaccgg acgctcctcg gttccggcac tggtcagggc
     2281 ggtccgttcg gtcgccagcg cgccgccgca gcgcagcgcg gtgttgcgga cctcggcata
     2341 gaggcagccc tgcagggtca gcgccccgcc ccgcccgctc aggccgttgg ccttgcggtt
     2401 tgtcagcttc agactgtcgc gggtttcgat ccgcagccgc tccaccaccg cgctggcaaa
     2461 gccttccagc cgcagcaccg tctcgccatg gcgggcgagc agcagggtgc cgtcgcccgc
     2521 gccggagatc ttgatgctgc gcgtaccgga aaggaccacc ggacggctgg tgcggtattc
     2581 gccccgctgg aagcacaccg cgatgtcctg gctgaaatcc atctgctcca gcagggtcac
     2641 ccagtcgctg ccttcgggga tgacataggt cgaacagccg ccgtggctgc cctcgcggtc
     2701 aaacccggcg gagaacagca gcggcggcgt gcccggctgg ggcccgtcgt ccagatcggc
     2761 gctgacctgg tgcagcgcgg tgggctgggc cgcgtcgatc gaccaggcca cctcgctgat
     2821 cccctggtcg ttggttggca cgaccaggtt ggcgcccgtc tgcgcaagat actccagccg
     2881 tccgccggtc ccggtcggcg ccaggatccg ccagcgcacc ggcgcccccg ccaccggggt
     2941 ttcgccccgc atcaccgcga cccgcagcat cgccggcagc cgggccagcc cgctggccgt
     3001 gcccggcgcc atctgcccgt cgccgccgcg gtagagcagt gtgcggtcgg tcagcgtcgc
     3061 cagcgggatg cgcaggtcgg tgaccccgct cgccgtgaca tgggccagcg gggcatagcg
     3121 gatttcgggc ccgtgcggcg cttcggcgac attggccagc gccccgtcgg cccaggccac
     3181 cgtgccatcg gcgcggaccg cgcaggtcca gtaatcgccg cgccggaacc gcgcggccgc
     3241 gccgccggtc agttccaccg ccagcccgtc gcccaggtcg atccgcagcg aatccgtgcc
     3301 gttggcggct agcgccaccg tggcgatccc gctggaggtg ccgccgctga ccgggaaggt
     3361 gtcccagcag cgcaggaacc agttcgcctc ctccgccggg gttgccgggg gtgcggcccc
     3421 ggtggcggcg acggtcgccc cgccgccttc ctgcaggaac acgtcggcgc catcgaccgt
     3481 gctgactttg aacagccgct ccgccgcggg cagcccggag ggattggcat cgtcgtggat
     3541 gctgaccagt tcggcatagg acccgggggc gaaccaggtc cgctcgtcga acggcggcgg
     3601 ggccacggtg atcttgttgc cgctgatccc gatcacccgg gccatgatgc tggcgttgcg
     3661 gcgcgagaac ttgagcttca atccattgag gccatagcgc ggcccgtcgg catgggtttc
     3721 cgccgcgacc ccgccgtgaa cctccacccg gtagagcaga ttttcgtgcc gcgaatagcc
     3781 gccttgcgga gtcagcgagc agatgtcggt cgggccgctg ggctgctggc ggcggaaggc
     3841 cagcttgccg gtcgaggccg ccttgatcgc ggtccaggcc ggatcggtca gcgggttggt
     3901 ggtggtcagc ggcaccggca gcacttgcca gtcgttcagc gcccggcccg gcgcctgggc
     3961 atcgccaagg gcgacgtcgg ccatggcccg ttcctcgacc ggatcgatgt gccgcaccgc
     4021 ggcgcgcagc gcgatcagca ccggcgcatt gggggccggg gtgaagggat gcggctggct
     4081 cgccagattg cagccatcgg gattttccag ccgccacccg tcaagatagc cctcacccgc
     4141 gccgatcgtc accgccagcg gcgtgccggc cgcgccgatc gcaaagccgg tcgtgcccgc
     4201 cggatagacc agcttgccgg gcgaccccat cagcgcccgg gcctccgcct cgacccggcc
     4261 aagcagcaga tcgccctgct ggttgagatc ggcatcaagc agcacctgcc cctgccgcgc
     4321 caccacaccg cgccgcatcc gcttgtcacg ctggggcacc cgcaggcggg tggcgtcgtt
     4381 gcgaagtgtc atggccggtt cctccgaaat gctaccgcgc tcgagcgggt gtcgtcgatc
     4441 agtccggcct cgttgccgaa cggtatgtag tcctcgaaca ggcgctccat gttggcctgc
     4501 cgcagcccca gggcaccggg cgaaaaggcc cccatctcgc tttcgccctc acccccgctg
     4561 atgatcgcgc gcgggctgtg cgcggcgagc atcgccacgg tcggttcgtc cagcgacagg
     4621 tcgagcagct gcggcgcaag gcccagttcg atcgcctcgc gctcgctcgg ccctagcggt
     4681 gcgcccttgg ccgccgcggc ggcggccagc gccatctgcg gctggcagcg gaaggtccgc
     4741 ggcacgctcg atcccggggc gatccagctg tagcgcacgc agccggcctg ggtccgggtg
     4801 acggttacgg gatcgcgcag cagcgcgttg gtcgcttcca gttccttgca gcggaccggg
     4861 ccgagcacgg tcacgttgtc gagccgggtt tccagctgga acgaatcgat cgcccggccg
     4921 gccgcgccgt cggcggcgac cacgctgtcg cgcacggcca gcgtgccggt ggcggtcgcc
     4981 cccgatgcat gaagccgcag cggcccggtc agcgagcggt cgatcgtgac cgccgcgccg
     5041 cgaaagggag ccgtggtgga aagcgccgcc ccgcccgggg cccgcaggga gcagaaccgg
     5101 atcagcagat cggagacagc cccggcccgc agctccagcc cggcggtcag gctcagcccg
     5161 tcgagcacca gccgcccgcc cagcgccggg ctgatctgcg tatcggcgat cagcggcacc
     5221 tcgaccacga agctgcgccc cttgcggacc agatagccgc gcagcgccgg atcgggatcg
     5281 accccggggc ggacctgcgg atcctcgaac cgcgaggaca ggatgtgaag ttccgacccg
     5341 cccggcacct tgaccgtgat cggcgccgag ccgcccgagc ggtcgcagga ggtgaggacg
     5401 atgtacccgc gccggttggg cccggcgagc ccgttccacg ccgccaccgc atcggccagg
     5461 ctggcggcct ggttgacccc ggccggcagc ggcaggtcgc gcgggccaac cagccagaca
     5521 tcggtctgga tgcccggcgc ggccaggatc gcgaagggca catcgggatc gttgcggtca
     5581 aacggccccg ccccgatttc ccgccccagg ccataggcat gggccacccg caccgcctcc
     5641 accggcaggc ccggcagcgg atcggccagg atcagccggc cggtgaccgg atcgaaggcg
     5701 caggtcaccg ggctgttccc ggtcttgtcg gtcgccccgc ttggcgatcc cgccacccag
     5761 cggtgcgccc gctgcgccgg cggccgcgca ccggccggca cgggcgggtc ttccagattg
     5821 gcgatgcaga tccgttcggg cggaacccgg tcaaacagca gcttgccgcc ctggcgccgc
     5881 cccagcagca cggcaaaggg ctcgccctgc gggttgaacc agcggatcgg ggtaagctgg
     5941 gcctcgcgct gttccagcgc ggcgtggcgc agctcttcca gctcctggta cagcggcagg
     6001 cgggtcagcc ggtcagccag gtcctgctct accatccggt ccgcgccgtc ctgcgagcgg
     6061 cgatcggggt tgaacagccg cagcggtgcc ccgggcactg ccgcaagctg gaaatagccg
     6121 gcctgggccg tgccgccagg cttccacggc gcggcgatcg gcaccccggc cacttcggtt
     6181 tcggtcagcg ccgcaaggtc tgacggagcc ggataggccg gagccagcgg ccggaccacg
     6241 tgcacgccga catggggcac atggtgcgcg ccgccggccc gggcgatcga gcggacatcg
     6301 accagccgcg gcagccggtc aaacccgctg ccggcgcggg cggcggtttc gccgggcacc
     6361 agccgggccg tggccgggcg ttcggggcgc agatcggcca ggtgcgcggt ccgcgccagg
     6421 cgcatgaagt attcgaccgc caccgcgccg aacccgccga catcggcggc cagctcttcc
     6481 agcacccggg ccgtgccctt gccccggcgg tagcgcagcg tgttggcgac ataggcgcgg
     6541 ctgttgtgcc cggccccttc gggcagcggg cgcagcggga tcgccccgat cagcgcggca
     6601 atctcgtcca gcgaggcggg ggaagccgtt tcggcgaaaa ggccatcgta aagcgcgtcg
     6661 atctcgccgt cgatttccgc cacgccgcgg gccagcaccc ccatcaaggc ttccagcgcg
     6721 tggccgcttt cggcatcgac cgcgcgaatg tgggccggca gcaggcccat cagccgggcg
     6781 gtttccgccg cgatccgcgc ttcgctgacc ggcttggtca tgccgccatc tcctcaaaca
     6841 gaacctgggc cggatcgatc gaaagcagcc cggccgggat cagatcgccg gtgcccggat
     6901 caaggatcgg cccggggcag agcagccgcc cttcgtcttc cgccggcatg ccgctgggca
     6961 gcgcgaacac ggtcagggtg gcggcgatca cgccgtccac gccttgcgcc gcggccagca
     7021 cctcgctgcg gtgcagggcc cgggcaaagg gccgggccgc ggggccaaag cgcttggcca
     7081 gcagggtccg caccgcctct tgcacgtcct tgcggcgaaa ggccggatcg caggccaggg
     7141 ccagggtgat ggcgggatgg atcgcggtga acccggcaat ccggaccgtg cgccccggca
     7201 cctgaacccg cttcagctcg cgccgcagcc cttcgaccac ggacgatccc tctgccggcg
     7261 attgcatctg gctgtcggcc agggtcaggc acacttcctg ccgcatcccg gcatagagca
     7321 gcgtggccag cgccttgccg accccgcgat aggccgcggc gaaggcctcg taatcggcca
     7381 ggctcaccac cttgtcgagc gtgcggaccg aaagcggcgc ggcgcggcgg atgtcctcga
     7441 tcgtttcggc atcgctgccg ccgtcggccg gcagcgggtt ggtgatgccg atcaccccct
     7501 gaaccggaac catcgccatg gtgatccgtc ccgccgccag gttgcccgcc ttgccgccgc
     7561 cggtgcggta cagagcggtg acgttgttga gcccgctggg caattgcccg gccagctgga
     7621 tcacggccgt gccatcgcgc cccggccgca cggtataggc gcggtcggcc gcgctcagcc
     7681 cggacaggtg cggcgcttcc ttccaggccc gcccgccgac ccgcacttcg accgcagggg
     7741 catagccgcg cggcgtttcg gccggaatgt aggccagcgg cttgcccttc agggcaaagc
     7801 gctgatcgcg cgcgccggcc ttgcccgatc cgatgatctc ctcgccctgg gccggagttt
     7861 cgccgtggct ggcggtgacg cagttgccca gcaccgccag gcgggtggag cggaagcgtg
     7921 ccgcgaccgg atcctcgaac agcagcgtgg tggtgccatc ggcgttgact tgcgcggtgc
     7981 ggacgatggc cacggcggcc tgctgcggcc cggtgacccc ggcctggccc atccacttct
     8041 cgcccgagag gatcacccgg cggccgggtg ccatctcgat cttaccctgc accaccagcc
     8101 ggtcgctttc gctgggcagc gcaatgtcgc gtggcgggcg gtaaagcgtc tggcgctcgg
     8161 tctccagaaa caccgccagt tcgcggacca gataacgcag gctggccgac ttcccggacg
     8221 ccgccttgcc gtcatcggac aggtcgatct ggtccagcgt gatccggctg accttcgccg
     8281 agagcaggaa atcggtgcgg gccagctcga ccgccttggt gatttcggtc agcgccggat
     8341 cggtgacctg gccggcggta tactcctcca tgtcgcgccg gcgttcctcg gccgcttcgt
     8401 ggtgggcgcg ggccagcgcg tcgttctctt ccatcgtttc gccggtgcgc acgaacttca
     8461 gtttggaata gtcgaacggc ggccgcttgt tggcgcgggt gaacagggcc aggcggcccg
     8521 cgaaggcttc gggataggcg gcgtcgagat cgatggtctt gcccccgctc gatccgggcg
     8581 cgccgacttc gaagttgtcc cactgggtcg ccccggtttc gggatcgtcg ctgatcaggc
     8641 cggcgcggac atcgtcggtc atcgcccggg catcgggcgc ggtggcgccg aaagcggcgg
     8701 cgcggcgggc caggatggcg acttcgccgg cccgcgccgc ggccagcgcg gcggtggtct
     8761 ccacatgcca gcaatcgctg agcgtgaaac gcaggaaggc cggatcgggc ggcagcgaaa
     8821 cccgctcgat cccggcgatc cggccaatca gccagtcgtc ggcaccgccg ccctggggca
     8881 ggcgcggcac ggccacgaaa tcgccctcgc gcgccgcgct ttccaccccg gcaatgcaca
     8941 gggtgcgggt ggaaccggtg atgtcgggca aggggcagac cgggcggatc gcgttccacg
     9001 ccccgaccgc ctcgaactcg gcatcggttt cgaacacctg cggcttttcg tcctgcccgg
     9061 ggaccgaggc gaccttggtg cccttggcca cgcggacctt ggccggggcg ccgggcaggg
     9121 tttccagcgt gaaggccagg gtcgtcgcgg ccgacagcgc cgggcgcggc tcgtacccga
     9181 tcagccggcc cagatcgacc agcgcatcgc ggtcctcgct gcgcagaatc gtgccgtcat
     9241 cggccagccg cgccccggtc caggccagca cgtggagact ggcggcctgg gcatcgatca
     9301 gcgcgatcgc cggatcgtcg ctgctgcgca cgcccagccg gtcgagcggg cgggccgtgc
     9361 cgacccgggc cgcggcgatg ctgtccagca tccgctgccg gatcgcggca tggcgaaagc
     9421 gcaggggatc gccgaccggc cgctgcggca cgccgcccgg tccgccgcaa ctggcgcagg
     9481 ttccgcagcc gcaaggctcg ctcatcgccc gcctcccatc tcaagcccga gccggccgcg
     9541 ttcgggaaag ctcgggtcgt cggccagttg cagcacttcc agcgggccgg gcgtgatcac
     9601 gccccgggcc agcgcatcgt ccggcgcaaa gccgagccgc tggaaccgcc gcggggtgac
     9661 gctttgcacg ccgtccacgg ccatgatcgc ggcgaccacc tgcgagaggt acagcggcga
     9721 tccgaaggtg aaattgtcgg gatggaagaa cccgcgctgg tcccccccgc cgggccgcag
     9781 cgccgcgcgg atcccggcgg cgaccgtgct gcgcagcgcg ttgggctcgg cgcagaccag
     9841 gaaggccagg tccagcgcga ccgtctgcgc gccgtgcagc gcgatatcga acccgatcag
     9901 ccggaaccgt tccatgtgcc gcaacaggtc gcggcggaac ggggcatcga gctccagccc
     9961 gccggtgcgg tcgatgtaga gcagcatggt ctgccaggcc ccggtccaga tcggctcggc
    10021 cacggcgttg gccacgcccg gatggcgcat ggcggcatcg gcatagtcct gcgccaccac
    10081 ggcccgttcc tgccgccgga acgcgatcgg ggcgttgatc cgcacctggc tggccggctc
    10141 ggccgcctgt ccgcccgccg ccgggagcgg gttgccgacc gtgacgctca gcccggcctg
    10201 gctggcgggc acgaccagat gggccagggc cccgatgccg atattgccct cgatcccgct
    10261 gccgaaccgg ccgcgcgcgc tgatccgcat gtccggatcg ggacacagcc cgttgatccc
    10321 gtcgccaaag cgcagcgcca ggtgcccgtc gatcgcggtt tcgagcacga attcgcggga
    10381 gaagcggctc gacttgagga ggtctggccg ggcctgccag gttccgaaat cgtcgagcag
    10441 ctcaaaggcc gcaaggcagg tggccggatc gaccgcggcg agcgcccggg ccgaggcatc
    10501 ggcatcgctg tcggtcagcc ggaacggccg cgtgcgggcc acgccgcccc ggtcgagccg
    10561 cggctgccaa tcgcagatgt cgccgggcgc gggcggatcg agccggggct ccagcgcgcc
    10621 ggcctcgctg ggcgtcaggc ccaggtgcgc tggcggaggc aacgagcggc cgtgatcggc
    10681 cagcatcagg ttggccgcgg cgcgggcgca ggtgaccatg ctggaactgc ccagcccgcc
    10741 cgcgacccgg gcctggatca ccagatcgaa cggcagcgcg tccgcttcgc cccattcggc
    10801 cagcaccagc ttcaagcccg gctgcaacac gtcgttgacc ggggtcacct tggtcagccg
    10861 caccacgtgg cggttggccg gccgggcatc ggcggcgacc ccggtggtgg gcgagacaat
    10921 ctcttccagc agcaggaaat cgcccttggc caggccgccg ctggcggcgc tggcatcgac
    10981 cagcgtggcc gcccggctgc cgcgcggcag ggtgcaggtc tcgtccgacc aggtgtgaag
    11041 cgcgatcgcg ttgcgccagc gccagacccg caagggggcc accgtttcga acaccggccc
    11101 ggccgtatcg cccagccggg catagtcccc cgccgggatc gcggtgcccg cgccttcgcg
    11161 ccggaccagc agcggggtgg cggaggcgat cacctggccg tcgctggccg cgtcctgcgg
    11221 atcgatccgc acgaagaccc gcgcgctggt cgcctcgccc ggcgtatagt caaccagccg
    11281 ggccagccgg ctgagcgagg tgcgactgcg cgcggtggac aggaacgcct cggtcccgac
    11341 ccagtcgagc ttgtagctga gctggtcggc gcggtaggcc agggcctcca ccagagtggt
    11401 ggtgaaatcg accggattgt cctcgcggaa gcccggcacc agcgcggcca tccggtccag
    11461 catcaggcgg cggaagccct ggtaatcgcg cacccggtaa tcgaactccg gatccgggcc
    11521 cagggcgtcg ggcggcggat cgcacggatc ggcgcaatcg aaatcgcccg cgcagccgac
    11581 cttgaacgaa aagtcgatgg ccgccagccg gccgtccaga aaggccggcg gggccaggct
    11641 gtcggccgca gcgacgatcc gcaggacata ggccgaaaag tcggtcggct ggccgggttc
    11701 gatttccagc acgtacttgt cgaccggatt gggcagcggg gtcagcagct tgaagcgggt
    11761 cggagcggga aagcgggtgc cgccggtcag gcggaaattg gccgtggtca gcgccgcggc
    11821 ggccagcggc atcggcttga ccaggtaaat atcgatgaac accggatcgg ccaggctggt
    11881 gccggtgcgc acctcgacat agtcgatccc cgtgaacggc acgcccggcg cggccagcag
    11941 ggtccgccgc tgctcgtcgc agcacagata gcggtcggtg ttcatgtcag gtccttgcgc
    12001 accgcgacgc gccccccggt cttggtcgcg cggatctcat aggcgacgac cacctccagc
    12061 cgggcttcgg cctcgtcgaa gtcgaccgac aggccctgca gttccagcac ctgccccagc
    12121 cactgggtga tcgtggcctg gagcgaagcc tccagcgcca gcgcggccgg gctgttgccg
    12181 gcctggaaca ccatctgccg caccgggctg cccagatccg ggcgcatcac ccgttcgccc
    12241 accatggtca ggatcagcag ctccagcatc tgttcgacat gggcggcatc gccatagccg
    12301 accgtccggc tgcgcccgtc ggcgccgggg ccaaagggaa aggccagccg ggtcatgtcg
    12361 ccaccacccg catctgcgag ctggaaatca ccggcggccc gccggggatc tgctcggccg
    12421 aaaagcacgt cgcgacacac gggttgatca gcaccggcac cccgccggcc gtgacccggg
    12481 tggcgccgac catccatttg gtggtgacgc agggctgcgg cttggtggca gggcccggga
    12541 tgggaaccgt gaagggacag ccggcgacca tgccctgatc ggtcagcacc gccaccggca
    12601 tccccgaaac ggtcacccgc gggcttgccg caacgatgct gagcgacccg ccgtgggggc
    12661 aggtggcaac cgctgcgctg tgcaataccg gtccgggcat tccgggtcct ttccttggtc
    12721 tggccgtcag gtgatggtca gggctccgcc gttgatgatg accgaggggc cggagagcat
    12781 gatcgacgcc ccctgcccgt tcgaaatggt gatcccgctg gccccgaccg agatcatcgc
    12841 gccggccacg gtcttgatga agacctgctg ggccgggttg tcggacagcg tgacgctggt
    12901 cccggtggtg gtctgcaggt ggatgttggg ggtggccggg gtcgccccgg ccagggcctg
    12961 ggtcggaaag ccggccggat cgggccagaa gctgccggtc cagatcgggt agtcgggatc
    13021 gcccgcctcg aactccaccc agaccatcga gccgatcgcc ggcagggcga cgatcccgga
    13081 catgcccttg cccgcgaact gcagcgaggg cagcgcccag gtcgagggaa acggaccata
    13141 gcggtcgccc agctggacct ggatccgccc ctggctctgg gcatccaggt tgttcagcac
    13201 cgtcgcccgg tacttgccga aatagcgcgg cccggcggtc atagcggcac cgccggaacc
    13261 gtgctgatca ggccgtcgcg ctgcagctgg aaggcctgtt tccagctgcc ccggcccagc
    13321 gtgtgggtga ccgagcggac gtagtagagc ccgtcgtgcg ccagcccggc cccgcgcaca
    13381 ccgatgatcg agcgcgcgtc gatcgggctg cccgaccggc tgagatcgat ggatccgttg
    13441 gccgaaaccg gctcggccgc cgcccgcccg gccagcagca gcgccatcgc cttgggcatt
    13501 tcggtccgtt cggtttccac cgggcggatc ttggtgggga tcagggggcg cgcggccagc
    13561 ggcggggcca gcagcgagat gttgggcacc gggaccggca cggaaaagcc gccgatcttg
    13621 acgtagcagt atggcatgat ggcggcattg ccatcggcgc tgaagctcat cgattcgatg
    13681 ttgctggcgt gatccatgtc gatcgtcagc gccggctgcg gcaggccgac ccgcagctgc
    13741 gggccccaat aggcggtgtt ggtcccgggt gccgggccgg gcgtcacgta gaattcgtag
    13801 cccgcctcct tggccagctt gttgaggtat tcaaagtccg tgccctgctg gtgggcatag
    13861 gatttgatcg gcagctcaac cgtgctgaaa acctccggga ccggcaccgg gatcaccccg
    13921 aacatggcgt atttggccag caaggccgcc acccgggcaa aggtcggcat ggccggaaag
    13981 ggaaacccgg tgaagtcgat caggtccatg tagcccgaga catcctcgcc ggtgatcgtc
    14041 agccggtttt cccccggcgc ggacgaggcg accacgtcct ggcgcttgat cgggccatcg
    14101 gccagcacct tgggcacgcc gttcagcgtg gcgaccagga tcacccggat catcggatcg
    14161 aagaacccgg ccggcagcag ggtggtggcg atcggcgagg tcttggaata ggtgaagctg
    14221 atctggaagc ccgaacgctg gccgaccgcg gaggtgacct gggcgctttc cagcgcctcg
    14281 atcacggcga ccggcaccgg ggccaccgcg accggcccca tcagcagggt cagggtcacg
    14341 cccttaagtg tcgccattgt cgcccatctc cagcgggagg gggatcagga tcagccgtcc
    14401 ggccggcccg gcagcctcgc ccggatcggc gtcgccgccg gcatcgcaga tccgccagta
    14461 gagctccgga tcgccatagc tggccgcggc cagcccatcg atccgctcgt cccccgccgc
    14521 gcggtgccgg tcgagcgcct ggtagcgccc ggtctgcggg atgatccgcc ggccgacaaa
    14581 ccggcgcgtc gtgccgtccg gcagcgtggt ttcacgcagg ggcacgccgg catagcggct
    14641 gttggggggg aaggtgtcgg tcatggtgtc cccgtcaggc ccaggccctg aaggcttggc
    14701 ggcttgcgtt tggaaagggc atctcggcgg cgggccgcgg tcatgaacag ctgcgcgccc
    14761 ttgctgccaa aggcgaggtc gtcgatcgac agcacccgca tgcccagcgc gatcttggcc
    14821 cggatcgggt tgagccgggt gtcgtgggct tcctccacca ccgaaaactc ggtgatccgc
    14881 accggcagca ggcggttgcg gcccagcacc agcacgatca tcggcgaggg cagcggcagc
    14941 acttccagcg tgccggtccg ggccagcgca tcggccgcct cgatcgccgc cggatcgggt
    15001 gagaggatcg cttccagctc ggccagctcg ggcgcgatcc cgtcttcgac cgtggccttg
    15061 ttctggcccg gatgctccag ccggtcggtc gcgtcgaact cgatatcgat cttgatcgtt
    15121 tcgaccggcg gcccgaccag ccgcagccct tccagccggt ccccggcatc gatcttcgcc
    15181 ccgcgcgggg tgagcgagcg ggtgaccgta tccgggttgt actgaaacgc gaccgtgcgc
    15241 tgcacggtct tgccctcggc atccatcagc acgagcccgc cgcgcagggt cttgggggat
    15301 ccggggaagg tggtcatggc tcggggctca tcttgctgcc cttgagaaca tcggcgacca
    15361 gttgcagcgg atcgaactct ttgggggtga tcgaaatctg ggaggggtcc ggccaggtta
    15421 cggtgccgtc ccgggcatag gcgatcttgc cgagctgaac gtcgagcgtc ggcccaagct
    15481 ggaccggccc gtactttgcc agcgggtggg tccattcgta gcgcagcaga ttgcgcgcca
    15541 gatagacagc cgccgcaaaa tcgatcccga gcgatccgta catcgcgccc gtcgcgaagg
    15601 cattggcctc gaagctgaag cccccgtcgg cgtactcggc cttgaccccg atgccgcctt
    15661 cgcccttcac gccgattgcc ggcttcaggt agattccacc ttcaacccct gccaatccgc
    15721 cggcaatcgc cacgcccagc aaggcgccga accggccgta cagctctgcc ttggcgggaa
    15781 ccgtgaactt gccatcgagg gtggccttga tcttcttgtc ttcttcgaac gggaacagtt
    15841 cggcggtcag cgtaccgttg ataaggcccg gaccgacacc gaaagcgaca cccagttcgc
    15901 cggttacgga aagggtggcc tgaacaaccg ggaacggcgg tatgggaatg ccgatgctga
    15961 tcccgccctt caggattgat cgacggtcct tgcccggacc cggccaggcc ttgaagacct
    16021 ggaagttggt gaccgtcacc gaacccttga ccttgatcct gccgtcttcg agcaagtgca
    16081 cgcccagttc cgggcggaac gcgggcgtca cctgataggc cagaatgccg gagccggaga
    16141 gcttgccttc cttgctgtag ttcagcttga aatcggcatc ggccttgccg cccagcatcc
    16201 cctttttctg gatgtgcgca ctgccgctga aggttccgtc ttcgccatcc ttctggtcgt
    16261 aattgacctg catccggccg gtgaagccgg cgacagaaag gttgccgctc ccgccaagat
    16321 gcaacaagcc gtcgccatag gatccgttga agtcgacttc gtcgaccagc ggcaacggct
    16381 tcttgatctt gaccccacct ttgtagctga ctccggcgcc tttccagcct gctttcaagg
    16441 tcagatcggc gtcgcggatc ttgcagctga tcccgccggt gccataaaag ccgagcaccc
    16501 ccgccgtttc cgcaaagcca aaggcgatat cggcggtcgc acccttgaaa ggcgtcccgc
    16561 tggccttggc ttcgcccgcc cagccgcgcc cccagacata gctgacgacc ccgtctgcgg
    16621 cggcaccccc ggggattgcc acttcgggct ggatcgtgcc c
//
LOCUS       DASUDV010000412         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_676557,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000412 DASUDV010000000
VERSION     DASUDV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676557"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..2780
                     /locus_tag="VFV30_08980"
     CDS             69..2780
                     /locus_tag="VFV30_08980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX4848266.1"
                     /translation="MPAALPLPAIHASHSGCWLRDGAGSTRGVAKGDAIVAAADTPLL
                     MLNAPLVASRLGYPDLSGLDLLELFAFIHPARFVVPTPKGLAQALDLEAPASDDGVPA
                     LLQQAAEALLTTCQDAGWAEREGAWSALQNLTRLRWPWVPLLAARLSAPERAERWLFA
                     RLPEWEEAPERPQPGQVVLDPADVAARLADLTGAGAELRPTQRAYAQEAAGVFQPRRR
                     EGLPHLLLAQAGTGIGKTLGYLAPASLWSEQAGGTVWVSTYTKALQRQLRRESSRAWP
                     ARRADGSRPVVVRKGRENYLCLLNLEDALQGGFAGRAGVLAQLVARWAAYSQDGDMIG
                     GDLPGWLPTLFRQRGIRALTDQRGECVYAGCPHYRKCFIERAARASAQADLVIANHAL
                     VMVNAARGRDQAARPTRLVFDEGHHVFEAADSTFAAALTGAETIELRRWVIGPERGSK
                     GRRRGLSARLADVASYDEAGGKAIAAARHAAEALPGDGWLQRLNEGCPSGPIEELLAA
                     VRATVHARDESGGQEAGYDLETEAAQLDGPLIELAGQAQIALADLRSPLIRLGVRLEA
                     LLAEPPDWLDGPGRARIEGARHSLAWRIDMLAAWEALLERLGGPADPEFVDWLAVERA
                     EARELDVGIHRRWLDPMKPFARTVLEPAHGVMITSATLKDGNDWDSAVARSGAPHLGL
                     APQLSSFESPFDYARQAEVLIVTDVAKGDVGALAGAYGRLIEASGGGVLGLFTAIRRL
                     RAVHGRIADRLARGGLPLYAQHVDPIDTGTLVDIFRDDPRASLLGTDALRDGVDVPGH
                     SLRLVVMEQVPWPKPSILHRARRLAGGGSVHDDQIIRARLAQAFGRLIRSRDDHGIFV
                     VLSAAFPSRLLSAFPQGTPVTRLTLDEALQRVASGVSPEPDRHEERT"
     gene            2777..3319
                     /locus_tag="VFV30_08985"
     CDS             2777..3319
                     /locus_tag="VFV30_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4848267.1"
                     /translation="MKILGLVRHAKSDWHDPRARDFDRPLNERGRKGAAIIGKHIRDH
                     GVKWDRMIASPAIRCAETVEIACHATGQPVTALWDRRIYLASSATLLDLLREQQGDPK
                     TVLMVGHNPGLEDLIFDLVPDDGSSPLRDVVEEKFPTAAFAVLELDIEKWADISERSA
                     RLVHLVRPRDLDPALGPETV"
BASE COUNT          514 a         1120 c         1127 g          559 t
ORIGIN      
        1 gtctggtgat ttggaccagc cggcggcacg atgtttactt ttcgttccgg cattgccaga
       61 accgaaccgt gccagccgcg cttccccttc ccgcgatcca tgccagccac tcgggctgct
      121 ggctgcgcga tggcgcgggc agcacccgtg gggttgccaa gggcgatgcg atcgtcgccg
      181 cggccgatac gccgttgctg atgctcaatg ccccgctggt agcctcgcgg ctgggctatc
      241 ccgatctgtc cggacttgat ctgcttgaac tgttcgcctt catccacccg gcgcgcttcg
      301 tggttcccac acccaagggc ctggcccagg cgctggatct ggaggctccg gccagcgacg
      361 atggcgtgcc cgccctgctc caacaggccg cagaggcctt gctcacaacc tgccaggatg
      421 ccggctgggc cgagcgcgag ggcgcgtgga gtgcgctcca gaacctcacc aggcttcgct
      481 ggccatgggt tccgctgctg gccgcccggc tatctgcgcc cgaacgcgcc gaacggtggc
      541 tgttcgcccg gctgcccgaa tgggaagagg cgcccgaacg cccacaaccg ggtcaggtcg
      601 tgctcgatcc cgccgatgtt gcagcgcgcc ttgccgatct gaccggcgcc ggggcagaac
      661 tgcgtcccac ccagcgcgcc tatgcccagg aggcggccgg ggtgttccag ccgcgccgcc
      721 gcgaaggttt gccgcatctg ctgctggccc aggccggaac cgggatcggc aagaccctgg
      781 gctatctcgc cccggcatcg ctgtggtccg aacaggcggg cgggacagtc tgggtatcaa
      841 cttataccaa ggcgctacag cgccagctgc gccgcgagag cagccgcgcc tggccggctc
      901 ggcgggccga tggctcgcgc ccggtggtgg tgcgcaaggg ccgggaaaac tatctgtgcc
      961 tgttgaacct ggaagacgcg ctgcaaggcg gttttgcggg ccgcgcgggc gtgctggccc
     1021 aattggtcgc gcgctgggcc gcttacagcc aggatggcga catgatcggc ggcgacctgc
     1081 ccggttggct tcccacgctg tttcgccaac gcgggatccg cgcgctgacc gaccagcgcg
     1141 gtgaatgcgt ttatgccggt tgcccgcact atcgcaaatg cttcatcgaa cgcgccgccc
     1201 gcgcctcggc ccaggctgat ctggtgattg ccaatcacgc gctggtcatg gtcaacgccg
     1261 cacgcggccg agatcaggcc gcgcgcccca cccggctggt gttcgacgag ggccatcacg
     1321 tgttcgaagc cgcggattcg accttcgccg ccgccctgac cggggccgag acaatcgagc
     1381 tcaggcgctg ggtgatcggt cccgaacgcg gttcaaaagg gcgcaggcgt gggctttcgg
     1441 ccaggcttgc cgatgttgcc agctatgacg aggccggcgg caaggcgatc gccgcggcgc
     1501 gccatgcggc agaggctctg cccggcgatg gctggctgca acgcctgaac gagggctgcc
     1561 cttccggccc gatcgaagaa ctgctcgccg ccgtgcgcgc cacggtccat gcccgcgatg
     1621 aaagcggcgg acaggaggcg ggctatgacc tcgagaccga ggcggcgcag ctcgatggcc
     1681 cgctgatcga actggctggc caggcccaga ttgccctggc agaccttcgc agcccgctga
     1741 tccgcctggg ggtgcggctc gaggcgctgc tcgccgagcc gcccgactgg cttgacggac
     1801 ctggcagggc ccggatcgaa ggggcgcgtc attcgcttgc ctggcggatc gacatgctcg
     1861 ccgcctggga ggccctgctc gaacggctgg gcggaccggc cgatcccgaa ttcgtcgatt
     1921 ggctggcggt ggaacgagcc gaggcgcgcg aactggatgt cggcatccac cggcgctggc
     1981 tcgatccgat gaagccattt gcccgcacgg tgctcgaacc cgcgcatggg gtgatgatca
     2041 ccagcgccac cctgaaggac ggcaacgact gggacagcgc ggtcgcccgc agtggcgctc
     2101 cgcatctggg attggcccca cagctgtcaa gcttcgaaag cccgttcgac tatgcccggc
     2161 aggccgaagt gctgatcgtg accgacgtgg ccaagggcga tgtcggggcg ctggccgggg
     2221 cctatggccg gctgatcgag gcttcgggcg gcggggtgct gggcctgttc accgcaatcc
     2281 ggcgactgcg cgccgtgcat ggccggattg ctgaccgact cgcgcgcggc ggcctgccgc
     2341 tctatgccca gcacgtcgat ccgatcgata ccggcacgct ggtcgatatc ttccgggatg
     2401 atccgcgcgc ctcgctgctg ggcaccgatg cactgcgcga cggggtagac gtgccgggcc
     2461 attcgctgcg gctggtagtg atggaacaag tgccctggcc caagccttcg attctccacc
     2521 gtgcccggcg cctggccgga ggcggatcgg tccatgatga ccagatcatc cgcgcgcgtc
     2581 tggcccaggc ctttggtcgg ctcatccgca gccgcgatga tcacggcatt ttcgtggtcc
     2641 tgtcagcggc cttccccagc cgcctgcttt cagccttccc gcaaggcacg cccgtcacgc
     2701 gattgacact cgacgaggct ttacaacggg tggcttcggg tgtttcacca gagcctgaca
     2761 gacacgagga gcgcacttga agattcttgg ccttgtccgg cacgcaaaaa gcgattggca
     2821 cgatccgcgc gcccgcgatt tcgatcgtcc gcttaacgag cgcgggcgca agggcgcggc
     2881 aatcatcggc aagcatatcc gcgaccacgg ggtaaaatgg gaccggatga ttgcatcgcc
     2941 cgcgatccgc tgtgccgaaa cggtggaaat cgcctgccat gcaacgggtc agccggtcac
     3001 cgcgctgtgg gaccggcgga tctaccttgc cagcagcgcc accctgcttg atctgctgcg
     3061 tgaacagcag ggcgatccca agacggtgct gatggtaggc cacaaccccg ggctggaaga
     3121 cctgatcttc gatctggttc ctgacgacgg atcaagcccg ctgcgcgacg tggttgagga
     3181 aaagttcccc accgccgcct tcgcggtgct cgaactcgat atcgaaaagt gggcggacat
     3241 ttccgaacgc tcggcgcggc tggtccatct ggtccgtccg cgcgatctcg atccggcgct
     3301 gggaccggag acggtctgaa
//
LOCUS       DASUDV010000413         6792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2112549,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000413 DASUDV010000000
VERSION     DASUDV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112549"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFV30_08990"
     CDS             complement(<1..579)
                     /locus_tag="VFV30_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4848268.1"
                     /translation="MIADQSIAAMIAAEQARYAEANPRSAAMAKEAARHWHRGVPFHW
                     MLDWGTPFPLFADRAVGAELWDVDGHRYDDFCLGDTGSMFGHSPPPVAEALARQGARG
                     LTYMLPTEDAVVVADELARRFKLPFWQVTGSASEANRAVIRWCRGITGRDKVLVFNGC
                     YHGAVDDVFVDLRDGVPELRRSLVGQVYDVRQH"
     gene            complement(576..1847)
                     /locus_tag="VFV30_08995"
     CDS             complement(576..1847)
                     /locus_tag="VFV30_08995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4848269.1"
                     /translation="MEPHAPSYYAATANPFPDQGALAGDASADVVVVGGGFTGLSAAL
                     HAAEAGFSVILLEGKRIGWGASGRNGGQMIPGMRWGAAELAERFGDGMARRLVGLANT
                     AGDRVKARIARHGIACDLRQGHFHAAAKPAHLDAMKREVETLQRLLGYEGAYTVEAGE
                     VERFVASPLYSGGMADGNGGHLHPLNYALGLARAALDAGVRIHEHSAVTAMDHTGPVL
                     ATTAQGRVTARFGVLACDAFMGGLDKRLGAMTMPVANYNVATVPLGEERARALIPSGA
                     AVSDSKFVLNYYRLSADGRLIFGGGEKYSPRPPADAAGFVRPYLEQVFPQLAGVGIDH
                     AWGGMVGVTLNRLPHFGRIGSSFFAHGWSGHGVLLTTLAGELIAEAMQGTAERFDLFA
                     NLPGRPFPGGPLLRHPLYVAGMLYYALRDRL"
     gene            complement(1850..3199)
                     /locus_tag="VFV30_09000"
     CDS             complement(1850..3199)
                     /locus_tag="VFV30_09000"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX4848270.1"
                     /translation="MSTDIASWIRDRGISEVECIVPDMNGIQRGKVLPANKFLKSLAD
                     KTLRIPGSVFIVTVTGGYPEDVEHIVPDFDPDVFLVPDPASLREAPGFKTPTAYVICD
                     AYKADGKAVDIAPRQILKKVLALYEAKGWQPVIAPEAEFYLVSKNLDADFPLTPPPGR
                     SGRAETASEAFGLEALSEFEDIIEQIYDWCEKAELNIDTMIHEAGAAQLEVNFIHGDP
                     LALADQVLLFKRIVRQVALEHGVYATFMAKPMADQPGSAMHIHQSVLSTETGRNVFSA
                     QNGKETALFRNHVAGLVRLMPQILPLVAPNVNSFRRMRPDTAAPINVHWGNDNRSCGF
                     RVPVSDAKDRRVENRLAGADANPYLAIAASLICGYIGMVERMVPPKIMEGNAYNRART
                     LPRTLEASLDRFYQCKPVKNLLGETFFEIFWAMKDAELYAYQSVISSWEREHLLLKV"
     gene            complement(3254..3970)
                     /locus_tag="VFV30_09005"
     CDS             complement(3254..3970)
                     /locus_tag="VFV30_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX4848271.1"
                     /translation="MTIKNLLAAAALGLPLALLPGIAQAQEAEEEAAGPIDVSAEVGL
                     FSDYRFRGVSLSGKDPEVTASLTVEHQSGFYASAWVSNVDIGTGSADDIEMDWTVGYS
                     RDVGNVTLDMGGIYYSYAGNSDLNYFEVYGSVGTKVGPADVKVGVAYAPSQDNIGGTD
                     NTYVYISGEVPIGDGPLSLHGTFGYENGAFGDHKKDWVVGASLDLGSGFTATADYVDT
                     ARSFSTAGDPTVVVSLKKSF"
     gene            4243..5556
                     /locus_tag="VFV30_09010"
     CDS             4243..5556
                     /locus_tag="VFV30_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4848272.1"
                     /translation="MPDPNDLSAFWMPFTDNRSFKANPRMFSAAEGMHYTATAGHKVL
                     DATGGLWCVNAGHGRARIVEAIREAAGALDFAPTFQLAHPKAFEAASRLAMIMPEGLD
                     RIFFTNSGSESVDTALKIALAYQRARGQGTRTRLIGRERGYHGVGFGGISVGGIVGNR
                     RQFGTLLTGVDHMRHTHDLARNAFSRGLPAHGAELAEDLERIVALHGAETIAAVIVEP
                     VAGSTGVLVPPQGYLQRLREICDTHGIVLIFDEVITAFGRVGKATAAERFGVVPDIIT
                     MAKGLTNAAVPMGAVAVRRSIHDTVVDSAAPGIELFHGYTYSGHPLASAAAIACLDLY
                     AEEGLFEKAIELEDYWADGVHSLRGARHVIDCRNVGLIGGIELEPRPGEPTKRAMDLF
                     RRAFDQGLLVRVTGDIIALSPPLILEKTHIDEIFGKLAQLLSELD"
     gene            complement(5553..6110)
                     /locus_tag="VFV30_09015"
     CDS             complement(5553..6110)
                     /locus_tag="VFV30_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848273.1"
                     /translation="MRRLLVPVLLSALASAACVAAQPAPEAGPAASPFYEMVVTAPGS
                     RSQGVRGTLYDAAGQPLPDVPTLEPVQTAIGPFDQIGCQYLWSVCGYLRRAEGFVPGG
                     PDNDPTATGPTLFRVSRTGPAAAPVYRGELFDGAAAVGRGSGRIVTPMGIFVWRDGPF
                     MGQGWSGWIPLDWLLADQRPQGPRR"
     gene            6225..6533
                     /locus_tag="VFV30_09020"
     CDS             6225..6533
                     /locus_tag="VFV30_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848274.1"
                     /translation="MNHARTRRLAALGLVCAGLAFAAHSPAASPSAPQCEDFSLPCKD
                     KDDIPPVPVPPVPSPSPEPVPSPEPAPEPSPAPSPEPSPEPSPEPSPEPSPDVNIPVR
                     "
     gene            6535..6732
                     /locus_tag="VFV30_09025"
     CDS             6535..6732
                     /locus_tag="VFV30_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848275.1"
                     /translation="MSPHLDRRSRSAPLLALGAALSLAMASAPASAQTGPLPPPPPVA
                     VSQSEPGHAVLREPGAVTTIS"
BASE COUNT         1071 a         2426 c         2241 g         1054 t
ORIGIN      
        1 gtgctggcgc acgtcataga cctggccgac caagctgcgg cgcagttcgg gcacgccgtc
       61 ccgcaggtcg acgaacacgt cgtccaccgc gccgtgatag cagccgttga acaccagcac
      121 cttgtcgcgc ccggtgatgc cgcgacacca gcggatcacc gcgcggttgg cctcgctggc
      181 gctgccggta acctgccaga acggcagctt gaagcgccgg gccagctcgt ccgccaccac
      241 caccgcgtct tcggtgggga gcatgtaggt cagcccgcgc gcgccctgcc gggccagcgc
      301 ctcggccacc ggcgggggcg aatggccgaa catcgatccg gtgtcgccca ggcagaaatc
      361 gtcatagcgg tgcccgtcaa cgtcccacag ctcggccccc acggcccggt ccgcgaacag
      421 cgggaacggg gtgccccaat cgagcatcca gtggaacggc accccgcggt gccagtggcg
      481 cgccgcctct ttcgccatgg cggcggagcg ggggttggcc tcggcatagc gggcctgttc
      541 ggcggcgatc atcgcggcga tcgattggtc ggcaatcaca ggcggtccct cagcgcgtaa
      601 tagagcatgc cggcgacata gagcgggtgg cgcagcagcg gcccgcccgg gaacggccgg
      661 ccgggcaggt tcgcgaacag atcgaaccgc tcggccgtgc cctgcatcgc ctcggcaatc
      721 agctcgccgg ccagggtggt cagcagcacc ccgtgcccgg accagccgtg cgcgaagaag
      781 ctgctgccga tccgcccgaa atgcggcaag cggttgagcg tcacgcccac catcccgccc
      841 caggcatggt cgatccccac gcccgcaagc tggggaaaga cctgctccag atagggccgg
      901 acaaagccgg cggcatcggc cggcggccgc ggactgtact tctcgccccc gccaaagatc
      961 aggcggccat cggcgctgag gcggtaatag ttgagcacga acttgctgtc cgaaaccgcc
     1021 gcgccagagg ggatcagcgc ccgggcccgc tcttcgccca gcggcacggt ggcgacgttg
     1081 taattggcga ccggcatggt catcgcgccc aggcgcttgt cgagaccgcc catgaaggca
     1141 tcgcaggcca gcacgccgaa gcgggcggtc accctgccct gcgcggttgt ggccaggacc
     1201 ggcccggtgt ggtccatcgc ggtcaccgcg ctgtgctcgt ggatccgcac cccggcatcg
     1261 agcgccgccc gggccagccc gagcgcgtag ttgagcgggt ggaggtgtcc gccgtttccg
     1321 tcggccatgc cgccggaata gagcgggctg gcgacgaacc gctcaacctc gccggcctcg
     1381 accgtgtagg cgccctcata gccgagcagg cgctgaaggg tctcgacctc gcgcttcatc
     1441 gcatcaaggt gcgcgggctt ggccgcggcg tggaaatggc cctggcgcaa atcgcaggcg
     1501 atgccgtggc gggcgatccg ggccttcacg cggtccccgg cggtgttggc caggccgacc
     1561 agccgccgcg ccatgccatc gccgaaccgc tccgccagct ccgccgcgcc ccagcgcatg
     1621 cccgggatca tctgcccgcc gttgcgcccc gatgcgcccc agccgatccg cttgccttcc
     1681 agcaggatga cgctgaaccc ggcctcggcc gcgtgcagcg ccgccgaaag gccagtgaag
     1741 ccgccgccca ccaccaccac atcggcactg gcatcgccgg ccagcgcgcc ctgatcggga
     1801 aagggattgg cggtggccgc gtaataggac ggcgcgtgcg gctccaccgc tacaccttca
     1861 gcagcaggtg ttcgcgctcc cacgaggaga tcaccgactg gtaggcgtaa agctcggcat
     1921 ccttcatcgc ccagaagatc tcgaaaaagg tctcgcccag caggttcttc accggcttgc
     1981 actgatagaa ccggtccagc gacgcctcga gcgtgcgggg cagcgtgcgg gcgcggttgt
     2041 aggcgttgcc ctccatgatc ttgggcggca ccatccgctc gaccatgccg atgtagccgc
     2101 agatcagcga ggccgcgatc gcgagatagg gattggcgtc cgccccggcc aggcggtttt
     2161 ccacccggcg gtccttggcg tccgacaccg gcacgcggaa cccgcaggaa cggttgtcgt
     2221 tgccccagtg gacgttgatc ggtgccgccg tatcgggccg catccgccgg aacgaattca
     2281 cgttgggcgc caccagcggc aggatctgcg gcatcagccg caccagcccc gcaacatggt
     2341 tgcggaacag cgcggtttcc ttgccgttct gggccgagaa cacgttccgc ccggtctcgg
     2401 tgctcagcac cgactggtgg atgtgcatcg cgctgccggg ctgatcggcc atcggcttgg
     2461 ccatgaaggt ggcatagacc ccgtgctcca gcgcgacctg ccggacgatc cgcttgaaca
     2521 gcagcacctg gtcggccagc gccagcggat cgccgtggat gaagttcact tccagctggg
     2581 cggcgccggc ttcgtgaatc atcgtgtcga tgttcagctc ggccttttcg caccagtcat
     2641 agatctgctc gatgatgtcc tcgaactcgc tcagcgcctc cagtccgaag gcctcgctgg
     2701 cggtctcggc ccggcccgag cggcccggcg gcggggtcag cgggaaatcg gcgtcaaggt
     2761 tcttggaaac gagatagaac tcggcctccg gcgcgatcac cggctgccag cccttggcct
     2821 cgtacagcgc caggaccttt ttcaggatct ggcgcggggc gatatcgacc gccttgccat
     2881 cggccttgta ggcatcgcag atcacatagg cggtcggcgt cttgaacccg ggcgcttcgc
     2941 gcaggctggc cggatcgggg accaggaaca cgtccggatc gaaatcgggc acgatatgct
     3001 cgacatcttc gggatagccg ccggtgacgg tgacgatgaa cacgctgccg ggaatccgca
     3061 gggtcttgtc ggccagcgac ttgaggaact tgttggcggg cagcaccttg ccgcgctgaa
     3121 tcccgttcat gtccgggacg atgcattcca cctcgctgat cccgcgatcc ctgatccagc
     3181 tggcgatgtc ggtggacatt gtggttctct ttgggttggg agcggacggg cggacccgcc
     3241 cgctcccgct ctgtcagaag ctcttcttca gcgacaccac gaccgtcgga tcccccgccg
     3301 tgctgaacga acgggccgtg tcgacataat cggcagtggc ggtaaagccg ctgcccaggt
     3361 cgaggcttgc gcccaccacc caatccttct tgtgatcgcc gaaggcgccg ttttcatagc
     3421 cgaaagtgcc gtgcagcgaa agcggtccgt caccgatcgg gacttcgccc gaaatgtaga
     3481 cataggtgtt gtcggtgccg ccgatgttgt cctgggacgg agcataggcc acgcccacct
     3541 tcacatcggc cgggccgacc ttggttccga ccgagccgta gacctcgaaa tagttgagat
     3601 cgctgttgcc ggcatagctg taatagatcc cgcccatgtc gagcgtgaca ttgccgacat
     3661 cgcgcgaata gccgaccgtc cagtccatct cgatatcgtc ggccgagccg gtgccgatat
     3721 cgacgttgga cacccaggcc gaggcataga agcccgattg gtgctccacc gtcagcgagg
     3781 cggtgacttc cggatccttg cccgagagcg aaaccccgcg gaaccggtag tcgctgaaca
     3841 ggccgacttc ggccgagacg tcgatcgggc cggcggcctc ttcctcggcc tcctgggcct
     3901 gggcgatccc cggcagcagc gccagcggca gacccagcgc ggcagcggca agcagattct
     3961 tgatggtcat ggcgttcccc tcatcccttg gcggttcttg ttgtccggac ctgcccggca
     4021 gacgccgtca ttcggcggtg tgctcttgcg gctgcgaccc gaagtctccc gggcagatct
     4081 ctttcccggt cgcaccttga cgccaatcgg cggcgaagac caatgggatt tattggagct
     4141 atgcctatcg ggatttccga taggcggttt cgccgcgaac cgaatggcct tggcgagcaa
     4201 ggccccctat cacagggccg cgcctttctt gccggagtca ccatgcccga tcccaacgac
     4261 ctgtccgcct tctggatgcc gttcaccgac aaccgcagct tcaaggccaa cccgcggatg
     4321 ttctcggccg cggaaggcat gcattacacc gccacggcgg ggcacaaggt gctggatgcg
     4381 accggcgggc tgtggtgcgt caacgccggc cacggccgcg cccgaattgt cgaggcgatc
     4441 cgcgaagccg ccggcgcgct cgatttcgcg cccacctttc agctggccca ccccaaggcg
     4501 ttcgaggcgg cgtcgcggct ggccatgatc atgcccgagg ggctggaccg gatcttcttc
     4561 accaattcgg gctcggaaag cgtggatacc gcgctgaaga tcgcgctggc ctatcagagg
     4621 gcccgcggcc agggcacccg cacccgcctg atcgggcgcg agcgcggcta ccacggggtt
     4681 ggcttcggcg ggatctcggt tggcgggatc gtcggcaacc gccgccagtt cggcaccctg
     4741 ctgaccgggg tcgaccatat gcgccacacc cacgacctgg cccgcaacgc cttttcgcgc
     4801 ggcctgccgg cgcacggcgc ggagctggcc gaggacctgg aacggatcgt cgcgctgcac
     4861 ggcgcggaaa ccatcgcggc ggtgatcgtg gaaccggtgg ccggatcgac cggcgtgctg
     4921 gtgccgccgc agggctatct ccagcgcctg cgcgaaatct gcgacaccca cggcattgtc
     4981 ctgatcttcg acgaggtgat caccgccttc ggccgggtcg gcaaggccac cgccgccgaa
     5041 cggttcggcg tggtgccgga catcatcacc atggccaagg ggctgaccaa cgccgccgtg
     5101 ccgatgggcg cggtggcggt ccgccggtcg atccacgaca ccgtggtcga cagcgccgcg
     5161 ccggggatcg aactgttcca cggctacacc tattcgggcc acccgctggc ctcggccgcg
     5221 gcgatcgcct gcctcgatct ctacgccgaa gagggcctgt tcgagaaggc gatcgaactg
     5281 gaggattact gggccgacgg cgtccactcg ctgcgcggcg cgcgccacgt gatcgattgc
     5341 cgcaatgtcg ggctgatcgg cgggatcgag ctggaacccc gccccggcga gccgaccaag
     5401 cgcgccatgg acctgttccg ccgcgctttc gaccaggggc tgctggtccg cgtcaccggc
     5461 gacatcatcg ccctgtcgcc gccgctgatc ctggaaaaga cccacatcga cgagatcttc
     5521 ggcaagctgg cgcagctgct ctccgaactg gactagcggc gggggccctg gggccgctga
     5581 tcggcaagca gccagtccag cgggatccag ccgctccagc cctggcccat gaacggcccg
     5641 tcgcgccaga cgaagatgcc catcggcgtg acgatccggc cgcttccccg gccgacagcc
     5701 gccgcgccgt caaacagctc gccgcgatag accggcgccg ccgccggtcc ggtgcggctg
     5761 acccggaaca gggtcggccc ggtggcggtc gggtcgttgt ccggtccgcc cgggacgaac
     5821 ccctcggccc ggcgcagata gccgcagacg ctccacagat attggcaccc gatctggtca
     5881 aacggcccga tcgcggtctg gaccggctcg agcgtcggca cgtccggcag cggctgtccc
     5941 gccgcatcgt aaagcgtccc gcgcaccccc tggctgcgcg agcccggcgc ggtcaccacc
     6001 atttcataga acgggctggc cgccgggccg gcctctggcg cgggctgggc cgcgacgcag
     6061 gcggcgctgg caagggcgga cagcagaacc ggaaccagca ggcggcgcat cggcaatctc
     6121 ctctgcccgc aaccttgcgc cggaacgcgc cggggccacc atgatctgga tcaaggcaat
     6181 cccggtggag gtggtggcat tgtcgggaat gagaggagac cgccatgaac cacgcccgaa
     6241 cccgccgtct tgccgcgctg ggccttgtct gcgccggcct tgcctttgcc gcgcacagcc
     6301 ccgccgccag cccctctgcc ccgcagtgcg aggacttctc gctgccctgc aaagacaagg
     6361 acgacattcc tccggtcccg gtgccgcccg tgccttcgcc ctcacccgaa ccggtgcctt
     6421 cgcccgagcc ggcgcccgaa ccgtcgcccg cgcccagccc ggagcccagc ccggaacctt
     6481 cgccggagcc cagcccggag ccgtcgcccg atgtcaacat tccggttcgc tgagatgagc
     6541 ccgcatcttg accgccgcag ccggtccgct ccgctgctcg cgctcggcgc ggccctgtcg
     6601 cttgccatgg cctccgctcc ggccagcgcc cagaccggcc cgcttccccc gccgccaccg
     6661 gttgcggtga gccagagcga acccggccac gccgtgctgc gcgagcccgg cgcggtcacc
     6721 accatttcat agaacgggct ggccgccggg ccggcctctg gcgcgggctg ggccgcgacg
     6781 caggcggcgc tg
//
LOCUS       DASUDV010000414         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_36373,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000414 DASUDV010000000
VERSION     DASUDV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36373"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFV30_09030"
     CDS             <1..1095
                     /locus_tag="VFV30_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4848276.1"
                     /translation="PPPLPQVSPRDPVQIQYTSGTTGFPKGVVLHHHGITNNARHGFA
                     RMKMPRGATTLAIMPLFHTGGCGMAVLGAVQFGCRMVLMRQFDPQQANALIESHGVIG
                     MMGVPTMLIGMLEADAHTPRDFSGIEMAGSGGSMVPPDLIRRVMDRLGCKFYIVYGQT
                     ETSPLLTQTRPDDSFDDQCHSVGQAMPHTELSIRDPATGAIVPVDTVGEICARAYSLM
                     LGYHNDPEATARTIDAEDWLHTGDLGTMDSRGFVRVTGRVKEMIIRGGENLFPAEIEN
                     VLLEHPDLTEVAVVGVPDERFGEAVAAFVRMAEGASLDPGALIAHCRANMAAQKTPKH
                     WIAVTEWPLTGSGKIQKFALRDQWVEGKFG"
     gene            complement(1106..1600)
                     /locus_tag="VFV30_09035"
     CDS             complement(1106..1600)
                     /locus_tag="VFV30_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848277.1"
                     /translation="MTHSPSPLAQRRDRQILLVAAIVVVQAVAAVFFVADALGDAAEA
                     GVTRHILIEALIALALLAGVGLGAWHTRVLLAEARRREQALAVASGALADHIAQRFAD
                     WGLTAAESDVALFALKGCDAAEIAGLRGAAQGTVRAQLSQVYAKAGVSSQAGLVSLFF
                     EDLL"
     gene            complement(1614..>2283)
                     /locus_tag="VFV30_09040"
     CDS             complement(1614..>2283)
                     /locus_tag="VFV30_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX4848278.1"
                     /translation="LHPRRRWDPIVKLTHWGIVAAVIGNALVTEEGSGWHIWVGYGLA
                     ALLALRLLWGLIGPKEARFSAFPPSPARAAAHVRAIGRGEVVRHRSHNPLGALMVYAI
                     WGTLLVVTGSGIAMSGPPPADPAAVSGEHGEGGKAEGGAEEDKHDEASAIGALIIAPA
                     HADEGGEGGEGEGDEAFEEVHEIAVNLLYLLIALHLMGVIFETRRSGHEVVGAMLPGG
                     NRRA"
BASE COUNT          370 a          820 c          770 g          323 t
ORIGIN      
        1 ccgccgccgc tgccgcaggt cagcccgcgc gatccggtgc agatccagta cacctccggc
       61 accacgggct ttcccaaggg cgtggtgctg caccaccacg gcatcaccaa caacgcccgc
      121 cacggctttg cccggatgaa gatgccgcgc ggcgcgacca cgctggcgat catgccgctg
      181 ttccacaccg gcggctgcgg catggcggtg ctgggcgcgg tccagttcgg ctgccggatg
      241 gtcctgatgc gccagttcga tccccagcag gccaacgccc tgatcgaaag ccacggggtg
      301 atcgggatga tgggcgtgcc gaccatgctg atcggcatgc tggaggccga tgcccacacc
      361 ccgcgcgact tttccgggat cgaaatggcc ggatcgggcg gatcgatggt tccgcccgac
      421 ctgatccggc gggtgatgga ccggctgggg tgcaagttct acatcgtcta tggccagacc
      481 gagacatcgc cgctgctgac ccagacccgg cccgacgaca gctttgacga ccagtgccac
      541 agcgtcggcc aggccatgcc ccacaccgaa ctgtcgatcc gcgatccggc caccggagcg
      601 atcgtcccgg tcgacaccgt gggcgagatc tgcgcccggg cctacagcct gatgctgggc
      661 tatcacaacg atcccgaagc caccgccagg acgatcgacg ccgaggactg gctccacacc
      721 ggcgatctgg gcacgatgga cagccgcggg ttcgtgcggg tgaccgggcg ggtcaaggag
      781 atgatcatcc gcggcggcga gaacctgttc ccggccgaga tcgagaacgt gctgctggag
      841 catcccgatc tgaccgaagt ggccgtggtc ggcgtgccgg acgaacgctt tggcgaggcg
      901 gtggccgcct ttgtccggat ggccgagggg gcgagtcttg atcccggggc gctgattgcc
      961 cattgccgcg ccaacatggc cgcgcagaaa acccccaagc actggatcgc ggtgaccgaa
     1021 tggccgctga ccggatcggg caagatccag aaattcgcgc tgcgcgacca gtgggtggag
     1081 ggcaagttcg gttgagggtg accggctaga gcaggtcctc gaagaacagg ctgaccagcc
     1141 cggcctggct cgaaaccccg gccttggcat agacctggct cagctgggcg cggaccgtgc
     1201 cctgggccgc gccgcgcagc ccggcgatct ccgccgcgtc gcagcccttc agcgcgaaca
     1261 gggcaacgtc gctttccgcc gcggtcaggc cccagtcggc aaagcgctgg gcgatgtgat
     1321 cggccagcgc gccagaggcc accgccagcg cctgctcgcg ccgccgggcc tcggccagca
     1381 gcacccgggt gtgccaggcc cccagaccga cgcccgccag cagggccagg gcgatcagcg
     1441 cctcgatcag gatgtggcgg gtcaccccgg cctcggccgc atcgcccagg gcgtcggcaa
     1501 cgaagaacac cgccgccacg gcctgcacca cgacaatcgc cgcgaccagc aggatctgcc
     1561 ggtcgcggcg ctgggcgaga ggggatgggc tgtgcgtcat tcttgctccc ggatcaggcg
     1621 cggcggttgc ccccggggag catcgcgccg accacctcat gcccgctgcg gcgggtttcg
     1681 aaaatgaccc ccatcaagtg cagcgcgatc agcagataga gcaggttgac ggcaatctca
     1741 tgcacttctt cgaacgcctc gtccccctcg ccttcgccgc cttcgccgcc ctcgtcggca
     1801 tgggccgggg cgatgatcaa cgcgccgatg gcagaggcct cgtcgtgctt gtcctcttcc
     1861 gcgccgcctt cggccttgcc gccttcgccg tgctcaccgc tgaccgccgc gggatcggcc
     1921 ggcggcgggc cggacatggc gatgccgctg ccggtcacca ccagcaaggt gccccagatc
     1981 gcatagacca tcagcgcccc cagcgggttg tgcgaacggt ggcgaaccac ctcgccccgc
     2041 ccgatcgccc tcacatgtgc cgcggcccgc gccgggctgg gcggaaaggc gctgaagcgc
     2101 gcttccttcg gcccgatcag cccccacagc agccgcaggg caagcagcgc ggccaggcca
     2161 tagccgaccc agatgtgcca gcccgatccc tcctcggtca ccagggcatt gccgatcacg
     2221 gcggccacga tgccccagtg ggtcagcttg acgatcgggt cccagcggcg gcgggggtgg
     2281 agg
//
LOCUS       DASUDV010000415         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1162879,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000415 DASUDV010000000
VERSION     DASUDV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162879"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFV30_09045"
     CDS             <1..246
                     /locus_tag="VFV30_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX4848279.1"
                     /translation="FLVEMGDKTQIATIALAAHYHAVIAVTAGTTLGMMLANGPAVFL
                     GEAIEKRVSMKLTRTLAALLFLVLGLWQLAEIYGLLG"
     gene            288..1553
                     /locus_tag="VFV30_09050"
     CDS             288..1553
                     /locus_tag="VFV30_09050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX4848280.1"
                     /translation="MWLLDKMLRAVIRDGQLRVIDHDGSEHRYGDPAADPVTIRFTDK
                     GAALHVARDPRVGAGEAYMDGRLVVEPPHDIRDMVLLVMANANRRKGGMVPPNPLKRG
                     IDWIFAKADQINLRAKASSNVVHHYDLTRPFYELFLDEDRQYTMAYYTDPANTLERAQ
                     LDKKALIAAKLRLEPGMRVLDIGCGWGGLGLYLHRHFGVEVLGVSLAPDQVAFANERA
                     EAAGVAGQVRFQLTDYRDVTGPFDRITSVGMIEHVGAPHLPEYFAKAHDLLADDGIML
                     THTIGRTNGPGTTDKWTRKYIFPGGYIPALSELVSACETTGWEIADIEVLRYHYAHTL
                     AEWYRRTTLHEAEITALYDARLFRMWQFYLAGAEQSFRHGNMVNFHIQSVKSRSAVPM
                     TRDYIGAEAARLSALDTAPVWHLERQGAE"
     gene            1550..>2429
                     /locus_tag="VFV30_09055"
     CDS             1550..>2429
                     /locus_tag="VFV30_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848281.1"
                     /translation="MKRIYGAFAAGLLALVTPALAQDENELASGEHEFICPVGGERFR
                     QDTSFPTFPLESYPDGGGPGVEGSDAGLPECPSNGLVLLVPYKHEQGDSQEAYTPEEI
                     ARLPALIASPEYQALRKDTRAARLVWLAEKLGRPAWQRFHLLQRAAWVARTPEERRRW
                     MTRIANEGEGLAASPGFPPVFAIEVRYYTVNALRELGRFDEASAMLARLRNDALAQRR
                     AERGDQPPNPHVMETLDTDSLAGIEELQKAIDLRDDDRFPVSMMGEKWANLVCGDADD
                     SPIPITEKTRQGCARRK"
BASE COUNT          421 a          818 c          805 g          385 t
ORIGIN      
        1 ttcctggtcg agatgggcga caagacccag atcgccacga tcgcgctggc cgcccattac
       61 cacgcggtga tcgcggtcac tgccggcacc acgctgggca tgatgctggc caacggcccg
      121 gccgtgttcc tgggcgaagc gatcgagaag cgggtctcga tgaagctgac ccgcaccctg
      181 gccgcgctgc tgttcctggt gctgggcctt tggcaattgg ccgagattta cggcctgctc
      241 ggctgatagg gctttgccac cagcccgagc aggcgtagcc tgaccgtatg tggctgctcg
      301 acaagatgct ccgcgccgtg atccgcgatg gtcagcttcg cgtgatcgac cacgacggca
      361 gcgagcaccg ctatggcgat ccggccgccg atcccgtcac gatccgtttt accgacaagg
      421 gcgccgcgct gcacgtggcc cgcgatccgc gggtcggcgc gggcgaagcc tatatggacg
      481 ggcggctggt ggtggagccg ccgcacgaca tccgcgacat ggtcctgctg gtcatggcca
      541 acgccaaccg gcgcaagggc ggcatggtcc cgcccaatcc gctgaagcgc gggatcgact
      601 ggatctttgc caaggccgat cagatcaacc tgcgggccaa ggccagcagc aacgtggtcc
      661 accactatga cctgacccgt ccgttctacg agctgttcct cgacgaggac cggcaataca
      721 ccatggccta ttacaccgat ccggccaaca cgctggaacg cgcgcagctg gacaagaagg
      781 ccctgatcgc ggccaagctg cggctggaac cggggatgag agtgctcgac atcggctgcg
      841 gctggggcgg gcttggcctc tacctgcacc gccatttcgg ggtcgaggtg ctcggtgtct
      901 cgctcgcccc ggatcaggtc gccttcgcca acgaacgcgc cgaggccgcc ggagtggccg
      961 gccaggtccg cttccagctg accgactacc gcgatgtgac cgggccgttc gaccggatca
     1021 ccagcgtcgg gatgatcgag cacgtcggcg cgccgcacct gcccgagtat ttcgcgaagg
     1081 cccatgatct gctggcggat gacgggatca tgctgaccca cacgatcggc cgcaccaatg
     1141 gccccggcac caccgacaag tggacccgca aatacatctt ccccggaggc tatatcccgg
     1201 cgctgagcga gctggtttcc gcctgcgaga cgaccgggtg ggaaatcgcc gacatcgaag
     1261 tgctgcgcta tcactatgcc cacaccctgg cagaatggta ccgccgcacc accctgcacg
     1321 aggccgagat caccgcgctt tacgacgccc gcctgttccg gatgtggcag ttctatctgg
     1381 ccggggccga acagagcttc cgccatggca acatggtcaa ctttcacatc cagagcgtga
     1441 aaagccgcag cgccgtgccg atgacgcggg attacattgg tgccgaggcc gcgcggctct
     1501 cggcgctgga cacggcgccg gtttggcatc tggaacggca gggggcggaa tgaagcgtat
     1561 ctatggagcc tttgcggctg gcctgctggc cctcgtcacg cccgcgctgg cgcaggacga
     1621 gaacgagctt gccagcggcg agcacgaatt catctgcccg gttggcggtg agcggttccg
     1681 gcaggacacc tcgttcccca cttttccgct ggagtcctat cccgacggcg gcggaccggg
     1741 ggtggaaggc agcgatgccg ggttgcccga atgcccttcg aacgggctgg tcctgctggt
     1801 gccctacaag cacgagcagg gcgacagcca ggaagcctat acgcccgaag agatcgcccg
     1861 cctgcccgcg ctgatcgcca gccccgaata ccaggcgctg cgcaaggaca cgcgcgccgc
     1921 tcggctggtc tggttggcgg aaaagctcgg ccggccggcg tggcagcggt tccatctgct
     1981 gcagcgcgcc gcctgggtcg cgcgcacgcc cgaggaacgc cgccgctgga tgacccggat
     2041 cgccaacgag ggcgaaggcc tggccgccag ccccggcttt ccgcccgtct tcgcgatcga
     2101 ggtccgctac tacacggtca atgccctgcg cgagcttggc cggttcgacg aagccagcgc
     2161 catgctggca cggttgcgga acgatgccct ggcccagcgg cgcgccgaac gcggcgatca
     2221 gccccccaac ccgcacgtga tggagacgct cgacacggac agtctcgcgg ggatcgagga
     2281 gctgcagaag gccatcgacc tgcgcgatga tgaccgcttt ccggtttcga tgatgggtga
     2341 gaagtgggcc aatctggtct gcggggatgc cgacgattcg ccgatcccga tcaccgagaa
     2401 gacccgtcag ggctgcgcca ggcgcaagg
//
LOCUS       DASUDV010000416         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_831251,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000416 DASUDV010000000
VERSION     DASUDV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831251"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..653
                     /gene="rdgB"
                     /locus_tag="VFV30_09060"
     CDS             24..653
                     /gene="rdgB"
                     /locus_tag="VFV30_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX4848282.1"
                     /translation="MTRKLGGETLVIATHNAGKLKEIGALLAPYGIACISAGSLGLPE
                     PAETGKTFVENAMLKARAAAEASGLPALADDSGLCVDALGGRPGVYTADWAERQWFEG
                     PPGRDWYMAMGKVEGMLQELGPETDRSCHFACVLAIAWPDGDHVVYEGRANGTLTWPP
                     RGTMGFGYDPVFVPLGDSRTFAEHEPAEKHGISHRADAFAKLVAEQFGA"
     gene            663..1814
                     /gene="hemW"
                     /locus_tag="VFV30_09065"
     CDS             663..1814
                     /gene="hemW"
                     /locus_tag="VFV30_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX4848283.1"
                     /translation="MARALYIHWPFCLAKCPYCDFNSHVRESVDHGAWQEALLADMRH
                     EAALAGGERLESIFFGGGTPSLMPPALVGRLLEEAERLWGFAKGIEITLEGNPSSVEA
                     GNYRDLASAGINRASLGLQALDDATLRFLGRLHGADEGLKAVEVAQSVFGRVSIDLIY
                     ARPGQAGAAWRTELERALALGTSHLSLYQLTIEPGTRFETMVRKGEFTPLDEDAAADL
                     FALTRSLTAAAGIPAYEISNHARPGEESRHNLTYWRYHDYAGIGPGAHGRRGGVATVR
                     HRKPENWLAAIAEHGHGIAETRQLPINEQASEAMLMGLRLDEGVDLAALAARFALAPA
                     ELCDPAKLAFYTGQGLTWQQGSRIGVSEAGMALLDALLAELVPAQLLAA"
     gene            1811..>2215
                     /locus_tag="VFV30_09070"
     CDS             1811..>2215
                     /locus_tag="VFV30_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX4848284.1"
                     /translation="MTRADILEAWHAHLAQGRRRSPHTVRAYVATAARLMDALGDPGW
                     PVLARLDSAALRGQLARRRADGLTNASAARELSALKGFIAFARQQAGMADPAPPRMRG
                     PRVKKGLPRPVTPDEAVHLAATVHEDAAEEWIG"
BASE COUNT          352 a          740 c          777 g          346 t
ORIGIN      
        1 gcgcaaggcg aggcggccgg caaatgaccc gcaaactggg cggcgagacc ctggtgattg
       61 ccacgcacaa tgcgggcaag ctcaaggaaa tcggcgccct gcttgcgccc tacgggatcg
      121 cctgcatctc ggccggatcg ctggggctgc ccgaacccgc ggaaaccggg aagacctttg
      181 tcgagaatgc gatgctcaag gcccgcgccg cggccgaggc ttcggggctg cccgcgctgg
      241 ctgacgattc ggggctgtgc gtcgatgcgc taggcggcag gcccggggtc tataccgccg
      301 actgggccga acggcagtgg ttcgaaggcc cgccggggcg cgactggtac atggcgatgg
      361 gcaaggtcga gggcatgctg caggagcttg gccccgaaac cgaccgttcg tgccacttcg
      421 cctgcgtgct ggcgattgcc tggcccgatg gcgaccatgt ggtctacgag ggacgcgcca
      481 atggcacgct gacctggccg ccacgcggca ccatgggctt cggctatgat ccggtgttcg
      541 tgccgctggg cgacagccgt acctttgccg agcacgaacc ggccgaaaag cacggcatca
      601 gccaccgcgc cgatgccttt gccaagctgg tcgcggagca atttggcgcc taggatcggc
      661 tgatggcccg cgcgctttac atccattggc cgttctgcct cgccaaatgc ccctattgcg
      721 acttcaacag ccatgtccgc gaaagcgtgg atcatggcgc gtggcaggaa gccttgctgg
      781 ccgacatgcg ccacgaggca gcgcttgccg gcggtgagcg gctcgagagc atcttcttcg
      841 gcggcggcac ccccagcctg atgccccctg ccctggtcgg ccgactgctt gaagaggccg
      901 agcggctgtg gggatttgcc aaggggatcg agatcaccct ggaaggcaat ccctcgtcag
      961 tcgaggcggg caattatcgt gatcttgcca gcgcagggat caaccgcgcc tcgctcgggc
     1021 tacaggcgct ggacgatgcg acgctgcggt ttctgggcag gctccacggc gcggacgagg
     1081 ggctgaaggc agttgaagtc gcgcagtcgg tgttcggccg ggtttcgatc gatctgatct
     1141 atgcccgccc tggccaggct ggggcggcat ggcggaccga actcgaacgc gcgcttgcgc
     1201 tggggacatc acacctgtcg ctctatcagc tgacgatcga gccgggcacg cggttcgaaa
     1261 cgatggtccg caagggcgag ttcaccccgc tggacgagga cgcggcggcc gatctgttcg
     1321 cgctgacccg ctcgcttacc gccgctgccg ggatccccgc ctacgagatt tccaaccacg
     1381 cccgcccggg cgaggaaagc cgccacaacc tgacctattg gcgttatcac gactatgccg
     1441 ggatcggtcc gggcgcgcat ggccggcgcg gcggcgtggc gaccgtgcgg cacaggaaac
     1501 cggaaaactg gttggcggcg attgccgagc acggccacgg cattgccgaa acccgccagc
     1561 tgccgatcaa cgaacaggcc agcgaagcca tgctgatggg cctgaggctc gacgaaggcg
     1621 tcgatctggc cgcgctggcc gcacgctttg ctcttgcccc ggcagagctg tgcgatcccg
     1681 ccaagcttgc gttctacacc gggcagggcc tcacctggca gcagggcagc cggatcggag
     1741 tgagcgaggc gggcatggcg ctgctcgacg cgcttctggc cgagctggtg ccggcccagt
     1801 tgctggcggc atgacccgcg ccgatatcct cgaggcctgg catgcacacc tggcgcaagg
     1861 ccggcggcgc agcccccaca ccgtgcgcgc ttatgtcgcc acggcggcgc ggttgatgga
     1921 tgcgcttggc gatcccggct ggccagtgct ggcccggctc gattccgctg cactgcgcgg
     1981 ccagctggcc cggcgtcgcg ccgatgggct gaccaatgcc agcgccgcgc gcgagctttc
     2041 ggcgctgaag ggatttatcg cctttgcccg gcagcaggcc ggcatggccg atcccgcccc
     2101 accgcggatg cgcgggcccc gcgtgaagaa gggcctgccc cgcccggtca ccccggacga
     2161 ggcggtgcac cttgccgcca cggtccacga ggacgcggcg gaggaatgga tcggc
//
LOCUS       DASUDV010000417         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1582548,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000417 DASUDV010000000
VERSION     DASUDV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582548"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="aroC"
                     /locus_tag="VFV30_09075"
     CDS             complement(<1..981)
                     /gene="aroC"
                     /locus_tag="VFV30_09075"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX4848285.1"
                     /translation="MSFNTFGHLFRFTTWGESHGPALGAVVDGCPPGLELSEGWIQPF
                     LDARRPGQSKFTTQRQEPDAVRILSGVFEGKTTGTPISLMIENVDQRSKDYSEVAKAY
                     RPGHADYAYDAKYGFRDYRGGGRSSARETAARVAAGAIARLVIPEVTITGYVSEIGGD
                     AIDPANFDAGEIGNNPFFCPDAAAALRWEKIVDDARLAGSSVGAVVECVASGTPAGWG
                     APLYAKLDAQLAAAMMGINAVKGVEIGDGFAAARLSGEANADPMRPGSDGPQFLANHA
                     GGIAGGISTGQPVVVRVAFKPTSSILTPQQTVTREGEAAEMFTKGRHDPCV"
     gene            1038..1349
                     /locus_tag="VFV30_09080"
     CDS             1038..1349
                     /locus_tag="VFV30_09080"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX4848286.1"
                     /translation="MYLTVFRNRKRPDMDQAAYQADNARMEELAASQPGFIAIKGFVA
                     EDGEVVAISEWESEAAARAWGSHPEHARVQARGRSEYYESYTLYSCVEPRVRSFERAG
                     Q"
     gene            1346..1690
                     /locus_tag="VFV30_09085"
     CDS             1346..1690
                     /locus_tag="VFV30_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEX4848287.1"
                     /translation="MSITLYGIPNCDTVKKARAWLDQRGLAYAFHDYKKAGADPAKLA
                     EWCESAGWEKVLNRAGTTFKKLPDADKQDLDQAKAIALMVANPSAIRRPVLEHSGGLL
                     VGFKADEWNQAL"
     gene            1672..>2094
                     /gene="pnuC"
                     /locus_tag="VFV30_09090"
     CDS             1672..>2094
                     /gene="pnuC"
                     /locus_tag="VFV30_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEX4848288.1"
                     /translation="MEPGAVIPLDPQVLTVIEWGALALGLVNIALLIFRNVWNFPFGI
                     GSVILAGFVMWERKLYGESSLQLFFVFAQLWGWWLWTRAGGEDSQVPVRWLDWPSRIV
                     WLVVTAAIALNMGWALDRFTDASVPYVDAGITGASIAAQ"
BASE COUNT          370 a          673 c          679 g          372 t
ORIGIN      
        1 gacgcaagga tcatggcggc ccttggtgaa catttcggcg gcctcgcctt cgcgggtcac
       61 ggtctgctga ggggtgagga tcgagctggt cggcttgaag gcaacgcgca ccaccaccgg
      121 ctggccggtg ctgatcccgc ctgcgatccc gccggcgtgg ttggcaagga attgcggccc
      181 atcgctgccc gggcgcatcg gatcggcgtt ggcttcgccg gaaagccgcg ctgcggcaaa
      241 gccatcgccg atttccacgc ccttgaccgc attgatcccc atcatcgccg cggcaagctg
      301 ggcatcgagc ttggcataga gcggtgcgcc ccagccggcg ggagtgccgc tggccacgca
      361 ttcgaccacc gcgccgaccg agcttccggc cagccgcgca tcatcgacga tcttttccca
      421 gcgcagggca gcggccgcat cggggcagaa gaacggattg ttaccgatct cgccggcatc
      481 gaaattggcc ggatcgatcg catcgccgcc aatttccgag acgtaaccgg taatcgtcac
      541 ctccgggatc accagcctgg caatcgcgcc agccgccacc cgcgccgcgg tttcgcgcgc
      601 cgaactgcgc ccgccgccgc ggtaatcgcg aaaaccgtac ttggcatcat aggcatagtc
      661 ggcatggccg ggacgatagg ccttggcgac ttcggaatag tccttcgagc gctgatcgac
      721 attctcgatc atcaggctga tcggcgtgcc ggttgtcttg ccctcgaaca cacccgacag
      781 aatgcgaacc gcatcaggtt cctggcgctg ggtggtgaac ttggattgac ccggccgccg
      841 cgcatcaagg aatggctgga tccagccttc ggacagttcc agtcccggcg gacagccatc
      901 caccaccgca ccaagtgcgg gcccgtggct ttcgccccag gtggtgaacc ggaataggtg
      961 gccgaaggtg ttgaacgaca tggcttcgct ttgccgcctg attgcccttg tgtccatatc
     1021 cggtttagga aggcgcgatg taccttaccg tctttcgcaa ccgcaaacgg cccgacatgg
     1081 atcaggccgc ctaccaggcc gacaacgcgc ggatggagga actggccgcc agccagcccg
     1141 gcttcatcgc gatcaagggc ttcgtcgccg aagacggcga agtggtcgcg atctccgaat
     1201 gggaaagcga agctgcggcc cgcgcctggg gcagtcaccc cgaacacgcg cgggtccagg
     1261 cccgcgggcg cagtgaatat tacgaaagct acacgctcta ttcctgcgtc gagcccaggg
     1321 tgcgcagctt cgaaagggct ggccaatgag cattaccctt tacggcatcc ccaattgcga
     1381 cacggtcaag aaggcgcgcg cctggttgga ccagcggggc ttggcctatg cctttcatga
     1441 ttacaagaaa gctggcgcgg acccggcaaa gctggccgag tggtgcgaaa gtgcgggctg
     1501 ggagaaagtg ctcaaccgcg cggggaccac tttcaagaag ctgcctgacg ccgacaagca
     1561 ggacctcgac caggctaaag cgatcgcgct gatggtggcc aatcccagcg ccatccgccg
     1621 tccggtgctc gaacattccg gcgggctgct ggtgggcttc aaggcggatg aatggaacca
     1681 ggcgctgtga tcccgctcga tccgcaggtg ctgaccgtga tcgaatgggg cgcgctggcg
     1741 ctgggcctgg tcaacattgc gttgctgatc ttccgcaacg tctggaattt cccgttcggg
     1801 atcggatcgg tgatcctggc cggcttcgtg atgtgggagc gcaagctcta tggcgaaagc
     1861 tcgctgcagc tgttcttcgt cttcgcgcag ctgtggggct ggtggctgtg gacccgcgcc
     1921 ggcggcgaag acagccaggt gccggtgcgc tggctcgact ggcccagccg gatcgtctgg
     1981 ctggtcgtca ccgccgcaat cgcgctcaac atgggctggg cgctcgatcg cttcaccgat
     2041 gcctcggtgc cctatgtcga tgccgggatc accggcgcca gcatcgccgc gcag
//
LOCUS       DASUDV010000418         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_500205,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000418 DASUDV010000000
VERSION     DASUDV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500205"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..649)
                     /locus_tag="VFV30_09095"
     CDS             complement(206..649)
                     /locus_tag="VFV30_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX4848289.1"
                     /translation="MTEDPKAFQLFTEIGIIDQLAGNAFERALPAGMTRAQFTVLHHL
                     HRRAAPSQSPAALADAIQVSRATMTSTLGRLAARGLVAIDPDPADGRGKQVRLTAAGE
                     AMRQDCIAAVAPLLPLVGTALAPTELDQTLALLRRLRERLDRARD"
     gene            complement(646..1764)
                     /locus_tag="VFV30_09100"
     CDS             complement(646..1764)
                     /locus_tag="VFV30_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848290.1"
                     /translation="MASRRQVILGLGGALVAGAAAGTWRVMRQPDSAIAPWQALSQRV
                     ADPRLDALRHAILAPNPHNRQPWLIRLDGSDSAVLTCDLAKRLPETDPFDRQITIGFG
                     TFIELAAIAASQRGYRLEVESFPEGEPQPRLDRRPVARLRWVADPALPRDPLAAAIVV
                     RRTNRQVYAPLPPGRLAALAEPGVTLSDDAELLARVARITVAAITGEMETPAAHMESV
                     NLMRIGAAEIDAMPDGLALSGPMIEATSLVGLTTRAALADPASTAYRLGLEDLQRTYG
                     SIPAALWITTPDNTRTSQIAAGRAYARATLRAARAGVAVHPLSQSLQEYPAMAAHFAQ
                     VHSLLAPGGGRVQMLARAGLAAPVRAAARYPLEAHLLT"
     gene            complement(1850..>2249)
                     /locus_tag="VFV30_09105"
     CDS             complement(1850..>2249)
                     /locus_tag="VFV30_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4848291.1"
                     /translation="NVNCRMFPGRSADETQALIEQAIGNPAIKVEQKVRGKPIAKAPP
                     MDPKLIGPMEALVAKYWPGVPVIPTMSTGATDGVYLAAVGIPTYGVPGEWGDPDGNGA
                     HGLNERLEVRSLYEGRDYLFDLVRALANKN"
BASE COUNT          344 a          754 c          782 g          369 t
ORIGIN      
        1 gttcgcgaat gcaacaacaa ctccccagga ggcagatatg cgcaacatcg cgcttatggt
       61 gctgccgatc ttcgccgctg cttcgctcag cggtctgatg ttctccgcca ccatcgccta
      121 agggcgctgg aacaggcggg acagcacaat cccaccgaag gccgccgcgc ccatcacggc
      181 gcggcggcct ttcgtttgtc aggcatcagt cgcgtgccct gtcgagtcgt tcgcgcaagc
      241 gccgcagcag agccagcgtc tggtccagct cggtcggtgc caaggccgtc ccgaccaatg
      301 gcagcagcgg ggcaacggcg gcaatgcagt cctgccgcat ggcttcgccc gccgccgtca
      361 gccggacctg cttgccgcgc ccgtcggccg ggtcgggatc gatcgccacc agtccgcgcg
      421 cggccagtcg gccgagggtg ctggtcatgg ttgcgcggct cacctggatg gcatcggcca
      481 gcgccgccgg tgactggctg ggcgcggcgc ggcgatgcag gtggtggagc acggtgaact
      541 gcgcccgggt catgcctgcc ggcaaggcgc gctcaaaggc gttgccggcc agctggtcga
      601 tgatcccgat ctcggtaaac agctggaagg ccttgggatc ctcggtcatg tcagcaggtg
      661 agcctccagc ggataacggg cagcggcgcg gaccggtgcc gccagtccgg cccgggccag
      721 catctgcacc cgtccgccgc ctggggcaag caggctgtga acctgcgcaa agtgcgcggc
      781 catggcggga tattcctgca acgactggct gagcggatgg actgccaccc cggcccgcgc
      841 ggcgcgcagg gtggcgcggg cataggcccg tccggcggcg atctggctgg tgcgagtgtt
      901 atccggcgtg gtgatccaca acgccgccgg gatcgagcca taggtgcgct gcaggtcctc
      961 cagacccagt cgataagccg tgctggcggg atcggcgagc gcggcgcgcg tggtgaggcc
     1021 aacgaggctg gtcgcctcga tcattggccc ggacagggcc aggccatcgg gcatggcatc
     1081 gatttcggcc gcgccgatcc gcatcaggtt gacgctttcc atgtgcgccg caggggtttc
     1141 catttccccg gtgatcgccg cgaccgtaat ccgggcgacc cgggccagca gttccgcatc
     1201 gtcgctcagg gtgacaccgg gttcggccag cgccgccagc cggcccggcg gcaagggcgc
     1261 atagacctgg cggttggtgc ggcgcacgac aatcgccgcc gccagcggat cgcggggcag
     1321 ggcgggatcg gccacccagc gcagccgggc gacagggcga cgatcaagcc gaggctgcgg
     1381 ttcaccctcg gggaatgact ccacctccag ccggtacccg cgctggctgg cagcgatcgc
     1441 ggccagttcg atgaaggtgc cgaacccgat cgtgatctgg cggtcgaagg ggtcggtttc
     1501 gggcaggcgc ttggccaggt cgcaggtcag cacggcgctg tcgctgccgt ccagccggat
     1561 cagccagggc tggcggttgt gcgggttggg ggccaggatc gcatggcgca aggcatcaag
     1621 ccgggggtcg gccacccgct gggacagggc ctgccagggc gcgatcgcac tgtccggctg
     1681 gcgcatcacc cgccatgtgc cggctgccgc accggccacc agtgcgccgc ccagcccaag
     1741 gatcacctgc cgccgcgatg ccatgcgtcc ctcctttaat tcgatatcga actagatagt
     1801 ttgatatcga agtagagtca aggcgcggtt gttcggccta gtttttggcc tagtttttgt
     1861 tcgctaacgc gcggaccaga tcgaacaggt aatcgcgccc ttcatagagc gagcgcacct
     1921 cgagccgctc gttcaggccg tgcgcgccgt tgccatcggg atcgccccat tcgccaggca
     1981 cgccataggt ggggatgccc accgcggcga gatagacccc gtcggtcgcc ccggtcgaca
     2041 tggtcgggat caccggaacc ccaggccagt acttggcgac aagcgcttcc atcggcccga
     2101 tcagcttggg atccatcggc ggcgccttgg cgatcggctt gccgcgcacc ttctgttcca
     2161 ccttgatcgc gggattgccg atcgcctgtt cgatcagcgc ctgggtttca tcggcgctgc
     2221 gccccgggaa catccggcag ttgacgttg
//
LOCUS       DASUDV010000419         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_765143,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000419 DASUDV010000000
VERSION     DASUDV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765143"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..902
                     /locus_tag="VFV30_09110"
     CDS             96..902
                     /locus_tag="VFV30_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4848292.1"
                     /translation="MSHKTIFITGGASGIGRAVAVKFGGQGWFVGLADIDEAGMRETA
                     ALLPAGKSSTHKLDVRDRAAWDEALDACARASGGRIDVVFNNAGIPLGGNLVENTPAE
                     IERCLDINLKGVLFGAQAAHPWLKASAPGSCLLNTASAAGIYGTPGASVYSATKFGVR
                     ALTECLDAEWHPDGIKVADLMPGFIDTPLIDKSPNASTNEDIRTRVTEAGLEITPVGE
                     VAEAAWKAVHGNDLHNRVGKTAHRIAFAARWMPGRLRKMTRSTGKPLGGR"
     gene            complement(916..1206)
                     /locus_tag="VFV30_09115"
     CDS             complement(916..1206)
                     /locus_tag="VFV30_09115"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX4848293.1"
                     /translation="MPIARLTETETAALLAEAPLWILREDGKALVRTFKFKDFNQAFG
                     FMTRVALYADKADHHPEWFNVYNRVEITLTTHDADGLSQRDVDMVRAIETML"
     gene            complement(1236..2309)
                     /locus_tag="VFV30_09120"
     CDS             complement(1236..2309)
                     /locus_tag="VFV30_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848294.1"
                     /translation="MKAIALPLLLLGTVQCAPSAQPQPHQGLWAGTVDGLEARLCFLS
                     GNSDGLEMVKIHYAGDTDIHELTRQNRERVFRETDYNGDAVSTMWIGPDRAGRLTLYR
                     HKGGKTSQAELQAVPVAEGGPGGCESAAFLAPRFPEGDRQTEPMEWDGHPYSRVTYRV
                     EPGYSDVAMTGIALDGSKPGDAAINARLEVAATSWGPGDDYRSCANRNRSMRVGIAFA
                     VELLALTDRYVSVRRDYSGSCGGSYDADISYWLLDRETGQKLDPESFLGPKAFKRGGN
                     PNSGLTESLAKRIAVPFTASEDTCYFNPEIDVVPPIAVHKDGLGFYRGRGEQEGCTPV
                     VVSWTEIAPYLTPAARRLLINAD"
     gene            2372..2950
                     /gene="ccmA"
                     /locus_tag="VFV30_09125"
     CDS             2372..2950
                     /gene="ccmA"
                     /locus_tag="VFV30_09125"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX4848295.1"
                     /translation="MQECRIAANDLACRRGDRLLFARLTFALEGGQALQLVGSNGIGK
                     SSLIRILAGLLPAFAGTVSRSGAIGLLDERPALDPHLPLGRALGFWQRIDGPADNDAA
                     RLGLAELMDVPVRYLSTGQKKRAALARLIGQRAPVWLLDEPLNGLDTRGVALVEELAA
                     AHCREGGIAVVASHQPFALPGMARIELKDFAA"
     gene            2947..3603
                     /locus_tag="VFV30_09130"
     CDS             2947..3603
                     /locus_tag="VFV30_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX4848296.1"
                     /translation="MSVILALLRRDLAQLLLGGRRGGALLPVLFFLAVAMLYPFAVGP
                     DAPVLARTGGGVIWVAALLAAILPLDRLIEPDLDLGLFDQWALRGISEEMLVAVRVLA
                     HWLSFGPPLMLAALPASALVGIDGQKLWLVELGLLAGTPGLAALGVLVAAITAGLRGG
                     AALGGLLAIPLAVPLLVFGAGSLAVGGEAGLGLAAAFSLVLLAITPFAAGAAIRAGRS
                     "
     gene            complement(3745..>3904)
                     /locus_tag="VFV30_09135"
     CDS             complement(3745..>3904)
                     /locus_tag="VFV30_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX4848297.1"
                     /translation="LGKPGTKMLEDGWTAVTRDRSLSAQFEHSIGITETGCEIFTASP
                     KGLDRPPY"
BASE COUNT          587 a         1314 c         1331 g          672 t
ORIGIN      
        1 cgcggcgcca ccgcgatctt cacctggcat tgctataaca acccggcact gtaatcactg
       61 cttacacaga tgtaagtccg attcgggagc ctgccatgag ccacaagacc atcttcatca
      121 ccggcggagc cagcgggatt ggccgggcgg tggcggtgaa gttcggcggc cagggctggt
      181 tcgtggggct ggccgatatc gacgaggccg gaatgcgcga gacggcggcc ctgcttcccg
      241 cgggcaaaag ctccacccac aagcttgacg tgcgcgaccg cgcggcctgg gacgaggcgc
      301 tggacgcctg cgccagggcg agcggcggca ggatcgacgt ggtcttcaac aacgccggga
      361 tcccgctggg cggcaacctg gtggagaaca ccccggccga gatagagcgc tgcctcgaca
      421 tcaacctgaa gggcgtgctg ttcggcgcac aggccgcgca cccctggctg aaggccagcg
      481 cgccgggatc gtgcctgctc aataccgcca gcgctgcggg gatttatggc acgccggggg
      541 cctcggtcta ttccgccacc aagttcgggg tgcgggcgct caccgaatgc ctcgatgccg
      601 aatggcatcc ggacgggatc aaggtggccg acctgatgcc gggcttcatc gacacgccgc
      661 tgatcgacaa gagccccaac gcctccacca acgaggacat ccgcacccgg gtgaccgagg
      721 cgggcctgga aatcaccccg gtcggcgaag tggcagaggc cgcgtggaag gctgtccacg
      781 gcaacgacct gcacaaccgc gtcggcaaga ccgcgcaccg catcgccttt gccgcgcgct
      841 ggatgcccgg ccggctgcgc aagatgaccc gcagcacggg caagccgctg ggcgggcgct
      901 gaggcccgcg ccgctttaca gcatcgtttc gatcgcccgc accatgtcca cgtcgcgctg
      961 gctgaggccg tccgcgtcgt gggtggtcaa cgtgatctcc acccggttgt agacgttgaa
     1021 ccattcgggg tggtgatcgg ccttgtcggc atagagcgcg acccgggtca tgaagccgaa
     1081 ggcctggttg aaatctttga acttgaacgt gcggaccagc gccttgccat cctcgcgcag
     1141 gatccacagc ggggcctcgg ccagcagcgc ggcggtttcg gtttcggtca ggcgggcgat
     1201 cggcacattc ttgctccagt gtttgcgcga cagtgtcagt ccgcgttgat cagcagtctg
     1261 cgggcagccg gggttagata gggcgctatc tccgtccagg aaacaaccac cggcgtgcag
     1321 ccttcctgct cgccccgccc gcggtagaag ccgagaccgt ccttgtgaac tgctatcggg
     1381 ggcaccacgt caatttcggg attgaaatag caggtatctt cgctcgccgt gaatggcacg
     1441 gcgatgcgct ttgccaggct ctccgtcagt ccggaattgg gattgccgcc ccttttgaaa
     1501 gccttggggc caagaaaact ttccggatcg agtttctggc cggtctcccg atcaagcagc
     1561 cagtaagaga tgtctgcatc gtacgatccg ccgcacgatc cgctgtaatc ccgccggacc
     1621 gacacataac ggtcggtcag ggccagcagc tcaaccgcaa acgcgatgcc cacccgcatt
     1681 gaacggtttc ggttcgcgca ggaccggtaa tcgtccccgg gaccccacga ggtcgccgcg
     1741 acctcaagcc gggcgttgat cgcggcgtct cccggtttcg aaccatccag cgcaatcccg
     1801 gtcatcgcca catcgctgta tccgggctca acccggtagg tcaccctcga atagggatgc
     1861 ccatcccatt ccatcggctc ggtctgcctg tcgccttcgg gaaatcgcgg tgcgaggaag
     1921 gcggcgctct cgcagcctcc cggcccgccc tcggccaccg gaaccgcctg cagctccgct
     1981 tgcgacgtct tgccgccctt gtggcgatag agcgtcaggc ggcccgcccg gtccggtcca
     2041 atccacatcg tgctgaccgc atctccgttg tagtcggttt cccgaaagac ccgctcccgg
     2101 ttctggcggg tcagctcatg gatgtccgta tccccggcgt aatggatctt gaccatttcc
     2161 aggccgtccg agtttccgga gagaaagcaa agccgggcct cgaggccgtc gaccgttccg
     2221 gcccagagcc cctggtgcgg ctgcggttga gcggacggcg cgcactgcac cgtcccgagc
     2281 aacaggagtg gcaacgcgat ggctttcata ccgcccctcc cgaccggaca atccttggca
     2341 ggcgcgcagc tttcccctat ccggcgagcc catgcaagag tgccgcatcg ccgcgaatga
     2401 tctggcctgc cggcgcggcg accgtttgct gttcgcgcgg ctcacgttcg cgctggaggg
     2461 cggacaggcg ctgcagctgg tcggctccaa cgggatcggc aagtccagcc tgatccgcat
     2521 cttggccggt ctgctgcccg cctttgccgg aaccgtcagc cgcagcggcg cgatcggcct
     2581 gctggacgag cgtcctgcgc tggatccgca tctgccgctg ggccgggcgc tgggcttctg
     2641 gcagcggatc gacggcccgg ccgacaacga cgccgcgcgg ctgggcctgg ccgagctgat
     2701 ggacgtgccg gtgcgctatc tttcgaccgg gcagaagaag cgcgccgcat tggcccggct
     2761 gatcggccag cgcgcgccgg tctggctgct ggacgaaccg ctgaacgggc tcgacacgcg
     2821 gggcgtggcc ctggtggaag aactggccgc cgcccattgc cgcgaaggcg ggattgcggt
     2881 ggtcgcctcg caccagccgt ttgccctgcc cggcatggcc cggatcgagc tgaaggattt
     2941 cgcggcatga gcgtgatcct cgccctgctg cgccgcgatc tggcccagtt gctgctgggc
     3001 gggcggcgcg gcggcgcgct gctgccggtg ctgttcttcc tggccgtggc gatgctctat
     3061 ccctttgcgg tcggtcctga cgcgccggtg ctggcgcgga ccggcggcgg ggtaatctgg
     3121 gtggcggcac tgctggccgc aatcctgccg ctcgatcggc tgatcgaacc cgacctggac
     3181 ctgggcctgt tcgatcagtg ggcgctgcgc ggcatatccg aagagatgct ggttgcggtg
     3241 cgggtgctgg cccattggct gagctttggc ccgccgctga tgctggccgc gctgccggct
     3301 tcggcgctgg tcgggatcga cgggcagaaa ctgtggctgg tcgaactggg gctgctggcc
     3361 ggcaccccgg gcctggccgc gctgggcgtg ctggtggcgg caatcaccgc gggcctgcgc
     3421 ggcggcgcgg cgctgggcgg cctgctggcg atcccgctgg ccgtgccgct gctggtgttc
     3481 ggcgcgggca gcctggccgt gggcggcgaa gcgggcctgg gcctggccgc cgccttcagc
     3541 ctggtgctgc tggcgatcac gccctttgcg gcaggcgccg cgatccgcgc agggcgaagc
     3601 tagtcctccc ccttggggga gggggaccac gaagtggtag aggggtacgt gcgagtgatc
     3661 gcggcctctc aggataagtc gggaccactg gggcgtgccc ctccaccacc ctgcgggtgg
     3721 tccccctccc cgttccgggg aggatcaata cggcggccgg tccagaccct tcgggctcgc
     3781 ggtgaagatc tcacagccgg tctcggtaat cccgatcgag tgttcgaact gcgccgaaag
     3841 cgagcgatcc cgcgtcaccg ccgtccagcc gtcttccagc atcttggtgc cgggcttgcc
     3901 caga
//
LOCUS       DASUDV010000420         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1714948,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000420 DASUDV010000000
VERSION     DASUDV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714948"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFV30_09140"
     CDS             complement(<1..665)
                     /locus_tag="VFV30_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4848298.1"
                     /translation="MKHDHRSFAPAAAAAYRPDASERIRRFVPMVRRLAWHVHGTGRA
                     GTEVEDLIQAGMVALTEAAQRHAGPGEDGFAAYAKLRVRGAMVDLIRRTVPLSRGGTE
                     KRRQLRDAKARLRGQLGRDATRAELAAAMGIGEAELEALRESSEPLRFESLDDAYSDS
                     NPSFADERPDSLEILAGEELREQLAEAIAALPERLQLVVQLYFVEELNLAEIAQTLQV
                     SVPR"
     gene            complement(662..2785)
                     /locus_tag="VFV30_09145"
     CDS             complement(662..2785)
                     /locus_tag="VFV30_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX4848299.1"
                     /translation="MLDNLRRIRVASLALPLGILALIGLMVVPVPALLLDLFFVLNIA
                     LSVAVLMAAMNAEKPLDFSSFPSVLLFATLLRLALNVASTRVVLVNGHQGGAAAGHVI
                     EAFGAFLVGGNFAVGLFVFMILMIINMVVITKGAGRVSEVSARFTLDALPGKQMAIDA
                     DLAAGLLTADEAKARRREVATEADFYGSMDGASKFVKGDAIAALLILGVNIVAGFCLG
                     MISHGLSAGEAAQVYITLAVGDALVAQVPALLLSIAAAVIVTRVADSRDLAGQIGGQL
                     ANPATWLPVAAILAAIGLIPAMPQTIFLPAALVAGWLWRRLAARAARPAPLVEEAPPA
                     EPGRIALDEVSDHTLVTIELGFGLVHLVDDRRGSPLVTRITGVRKQLSQTFGFVVPQF
                     RVRDSLDLAPNDYRILLGGVALGAGAILPDRILAIDAGDVRDGARLNGDATLDPSFGC
                     PAVWIDPAQRDHAIAEGYLTVDASTVIATQLNQLLGVRAPALFGPDELKAILDAVRDH
                     SAGLVDAVTPQPLSLAALTRLLRTLLEDGIPIGHPVPILSALALGLQQTSEHDRLVDL
                     VRADLGPLIVGRICAPGERLPVLTLDAQLEAAIVTGMADPVSGQPVIEPDLARSIGDY
                     VAALMAERGAAAPPPALVVQPRARRALAALLRLRAPACTVLSIAELPVSQPIEVVAVI
                     GASPRPAPGLPQPHILPAELEGMPA"
     gene            complement(2791..3600)
                     /locus_tag="VFV30_09150"
     CDS             complement(2791..3600)
                     /locus_tag="VFV30_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4848300.1"
                     /translation="MSETRSIGATVPATAQVRAAIARAAEATGVDFAYLLGQARLESS
                     LDPNARAATSSAAGLYQFTNGTWLATLGRHGAEHGLDWAEAAIARGDPGARGDLLALR
                     YDPQVAALMAAELASDNRAALRESLGREPDPAELYMAHFLGADGAKRFLAALEDDPLQ
                     SAATVLPKAAAANPAIFYNEGGEARSLGGVMDLMRGRLSSAMGGAQGGLAEQWAGVVP
                     PGGATPSGSAPLPPPPRASMAQTLAATFGGAGAAVPDHVRSAYARLQALGL"
     gene            complement(3657..4016)
                     /locus_tag="VFV30_09155"
     CDS             complement(3657..4016)
                     /locus_tag="VFV30_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEX4848301.1"
                     /translation="MSASAEMRDNLRQMIAVLQAERQALAGLDLDAIMGCAQDKIALC
                     GRLDDGGPFAAGAVIDEECRGLLDAARRLNEVNRQVRNLIAANVAARLDALTGSPALY
                     RAAGQGTAPYAYAGVRA"
BASE COUNT          671 a         1417 c         1367 g          562 t
ORIGIN      
        1 cgcggcaccg aaacctgcag cgtctgcgcg atttcagcca ggttgagctc ttccacgaag
       61 taaagctgga ccaccagctg caaccgttcg ggcaaggccg cgatggcttc ggcaagctgt
      121 tcgcgcagtt cttcgccggc caggatttcc aggctgtcgg gccgctcgtc ggcaaaggag
      181 gggttgctgt cggaataggc atcgtcgagc gattcgaagc gcagcggctc gctgctctcg
      241 cgcagcgctt caagctcggc ctcgccgatg cccattgccg cagccagctc ggccctggtc
      301 gcatcgcgcc ccagctggcc gcgcagcctg gccttcgcat cgcgcaattg ccggcgcttt
      361 tccgtaccgc cacgcgacag gggtaccgtg cgccggatca ggtcgaccat cgccccgcgc
      421 accctaagct tggcataggc ggcaaagcca tcctcgccgg ggccggcgtg gcgctgtgcc
      481 gcttcggtca gcgcgaccat cccggcctgg atcaggtcct cgacctcggt cccggcccgt
      541 ccagtgccgt gaacgtgcca ggcgagccgc cggaccatcg gcacgaaccg gcggatccgt
      601 tcgcttgcat cggggcggta ggccgccgcg gcggcgggtg cgaaactgcg gtgatcgtgt
      661 ttcatgcggg cataccttca agttcggcag gcaggatgtg aggctgcgga aggcccggag
      721 cgggccgcgg cgacgcgccg atcacggcga caacctcgat cggttgcgaa accggcaatt
      781 cggcaatcga aagcacggtg caggccggtg cgcgcagcct gagcaaggcg gccagcgccc
      841 gccgggcgcg cggctgcacc actagcgcag gcggcggtgc ggccgcgccg cgctcggcca
      901 tcagggcggc cacataatcg ccgatcgaac gggcaaggtc cggttcgatc accggctggc
      961 cgctgaccgg atcggccatg ccggtcacga tcgccgcctc aagctgggca tcgagggtga
     1021 gcacgggcaa gcgttcgcca ggcgcacaga tccgcccgac gatcagcggc ccgaggtccg
     1081 ccctaaccag atcgaccagc cggtcgtgct cgctggtctg ctggagtccg agcgccagcg
     1141 cggaaaggat cggcaccggg tggccgatcg ggatgccatc ttcgagcagg gtgcgcagca
     1201 gccgggtcag cgcggcgagc gagaggggct ggggcgttac ggcatcgacc agcccggccg
     1261 aatgatcgcg cacggcatcg aggattgcct tcagctcatc cggcccgaac agcgccgggg
     1321 cgcgcacgcc cagcaactgg ttgagctggg tggcgatcac ggtcgaggca tcgacggtca
     1381 gatagccctc ggcgatggca tgatcgcgct gggccggatc gatccacacc gccgggcagc
     1441 cgaagctggg gtcgagcgtc gcatcgccat tgagccgcgc gccatcgcgc acgtcgccgg
     1501 catcgatcgc caggatccgg tcgggcagga ttgcccccgc gcccagcgcc accccgccca
     1561 gcaggatgcg atagtcgttg ggggccaggt ccaggctgtc gcgtaccctg aattgcggaa
     1621 ccacgaagcc gaatgtctgg ctgagctgtt tcctgacccc ggtgatccgc gtcaccagcg
     1681 gcgaaccgcg ccggtcatcg accaggtgaa ccagcccgaa acccaattcg atcgtcacca
     1741 aggtgtggtc ggatacctcg tcgagcgcga tccgtcccgg ttcggcgggc ggcgcttcct
     1801 ccaccagcgg ggcaggccgc gccgcgcgcg ccgcgagccg gcgccacagc cacccggcaa
     1861 cgagcgcggc aggcagaaag atcgtctgcg gcatggccgg gatcagcccg atcgccgcga
     1921 ggatcgccgc aaccggcagc caggtggcgg gattggccag ctgcccgccg atctgcccgg
     1981 ccagatcgcg gctgtcggca acgcgggtga cgatgaccgc cgcagcgatc gacagcagca
     2041 acgccggcac ctgcgccacc agcgcatcgc ccaccgccag ggtgatatag acctgcgccg
     2101 cctcgcccgc cgaaaggccg tggctgatca tgccaaggca gaagccggca acgatattga
     2161 cccccaggat cagcagcgcg gcgatcgcat cgcccttcac gaacttgctg gcgccgtcca
     2221 tcgagccata gaaatcggcc tcggtcgcca cttcgcggcg gcgcgccttg gcctcgtcgg
     2281 cggtcagcag gccggcggca agatcggcgt cgatcgccat ctgcttgccc ggcagggcat
     2341 cgagggtaaa ccgcgccgaa acttccgaaa cgcggcccgc gccctttgtg atcaccacca
     2401 tgttgatgat catcaggatc atgaacacga acaggccgac cgcgaaattg ccgccaacca
     2461 ggaaggcgcc gaaggcttcg atcacatggc ccgccgccgc cccgccctgg tgcccgttga
     2521 ccagcaccac ccgggtcgag gcgacattga gtgccaggcg cagcagcgtc gcgaacagca
     2581 ggaccgacgg gaacgaggag aagtcgagcg gcttttcagc attcatcgcc gccatcagca
     2641 ccgccaccga cagcgcgatg ttgagcacga agaacaggtc gagcagcagc gccggcaccg
     2701 gcaccaccat cagcccgatc aaggcgagga tgccgagcgg cagggcaagg cttgccaccc
     2761 tgatccggcg caggttgtcg agcatcgcgc tcacagcccg agcgcctgca gtctggcata
     2821 ggcgctgcgc acatgatcgg gcacggccgc acccgccccg ccgaaagtgg ccgccagggt
     2881 ctgcgccatc gaagcacgcg gcggcggggg caagggcgcc gaacccgagg gcgtggcccc
     2941 gccgggcgga accactccgg cccactgctc tgccaggcca ccctgcgccc cgcccatcgc
     3001 cgagctcagc cggccgcgca tcaggtccat caccccgcca aggctgcggg cttctccgcc
     3061 ttcattatag aagatcgccg ggttggcggc ggcggccttg ggcaggaccg tggcggcgct
     3121 ttgcagggga tcgtcttcca gtgcggcaag gaagcgcttc gcgccgtccg cgccgaggaa
     3181 gtgcgccatg tagagttcgg cgggatcggg ttcgcggcca aggctttcgc gcagcgccgc
     3241 gcggttgtcg ctggccagtt cggccgccat cagcgcggcc acctgcggat cgtagcgcag
     3301 cgcaagcaga tcgccgcgcg cgcccggatc gccccgggcg atcgccgctt cggcccaatc
     3361 cagcccgtgt tcggcgccgt gccggcccaa tgtggccagc caggttccat tggtgaactg
     3421 gtagagcccg gccgcgctcg aagtggcggc gcgcgcattc ggatcaaggc tcgattcaag
     3481 ccgggcctga cccagcagat aggcgaagtc gacgcccgtc gcctcggcgg cgcgggcaat
     3541 cgcggcgcgc acctgcgcag tggcgggcac tgtggcgccg atggatcgtg tctcgctcaa
     3601 gccttgctcc cgatgcggtt ggcattcggg agcagttcag caagggccgt gccaaatcag
     3661 gcgcggaccc cggcataggc atagggtgcg gtaccctggc ccgcggcacg gtacagcgcc
     3721 ggcgagccgg tcagcgcgtc gagccgggcg gcgacattgg cggcaatcag gttgcgaacc
     3781 tggcggttta cctcgttcag gcgccgcgcc gcgtcgagca gcccgcggca ttcctcgtca
     3841 atcacggcac ccgcagcgaa gggcccgcca tcatccagcc gcccgcacag cgcgatcttg
     3901 tcctgcgcgc agcccatgat cgcatcaagg tcgagcccgg caagcgcctg ccgttcggcc
     3961 tgcagcacgg caatcatctg ccgcagattg tcgcgcattt cggccgaggc actcatt
//
LOCUS       DASUDV010000421         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_919822,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000421 DASUDV010000000
VERSION     DASUDV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919822"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFV30_09160"
     CDS             <1..384
                     /locus_tag="VFV30_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4848302.1"
                     /translation="EGKKNGVLEDNLRKDREVAQIQRDNFRKAHKAGVRMVFGSDAGV
                     MPHGQIGRQFATMVTYGMTPLDAIRAATLNAAMALGWENQVGTIAVGRYADFVAVDGD
                     PLANVRELESVDAVIKGGKRIEPAR"
     gene            complement(401..739)
                     /locus_tag="VFV30_09165"
     CDS             complement(401..739)
                     /locus_tag="VFV30_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX4848303.1"
                     /translation="MKSMEEMIKAAQQAAETISKQMGEMQGKLDEIEVEGKSGGGLVT
                     IRCTAKGRVIGVGIDDSLMQPSEKGILEDLIAAAFNDARAKADQVSGEEMARMQQGMG
                     LPPGFKLPGM"
     gene            complement(744..>2339)
                     /locus_tag="VFV30_09170"
     CDS             complement(744..>2339)
                     /locus_tag="VFV30_09170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX4848304.1"
                     /translation="KYRSQTFAELLGQDAMVQTLANAIKRDRLAHAFLMTGIRGVGKT
                     STARLIAKALNCIGPDGQGGPTIDPCGQCEPCVAIAEGRHIDVIEMDAASHTGVDDVR
                     EIIEAVRYAAVSARYKIYIIDEVHMLSRNAFNALLKTLEEPPPHVKFLFATTEVEKLP
                     VTVLSRCQRFDLRRIPAPLLAQHFANICDKEGVEADPEALAMIAAAAEGSVRDGLSIL
                     DQAIAHADLGGEGRVIAARVQDMLGLADKSALRRMFAAMLAGDGPALLDMVDQQFALG
                     VEPLALMRAGLDLVHKVTVAQLGRSGADGASAEERADIDQWAGSLSPGQLHRLWQLML
                     KGHDEVRSAPDPLIAAKMALLRVQHASDLPDPGTLARKLEEMAASGSFVAGTPASAEG
                     GTVAAAAPAPTALDWARIVDQVDQSGQLRLAQVMRDWVRVMHLAPTELTYVLAPGYPG
                     DPTPEIRDALLRATGERWQVTRAEGDAAPSLREAEEAARQASRAETLAHPLVEATMAA
                     FPRAELIDESPDARVVGKASNWR"
BASE COUNT          390 a          803 c          747 g          399 t
ORIGIN      
        1 gaaggcaaga agaacggcgt gctggaagac aacctgcgca aggaccgcga ggttgcccag
       61 atccagcgcg acaacttccg caaggcgcac aaggccgggg tgcggatggt gttcggatcc
      121 gatgccgggg tgatgccgca cggccagatc gggcgccagt tcgccaccat ggtgacctat
      181 gggatgaccc cgctcgatgc gatccgcgca gccacgctca acgccgcaat ggcgctgggt
      241 tgggaaaacc aggtcggcac gatcgcggtc ggccgttacg ccgatttcgt cgcggttgat
      301 ggtgatccgc tggccaatgt ccgcgaactg gaaagcgttg acgcggtgat caagggcggc
      361 aagcggatcg aaccggcgcg ctaggcaccg gcgccatccc ttacatcccc ggcagcttga
      421 accccggcgg caggcccatg ccctgctgca tccgggccat ttcctcgccg ctgacctgat
      481 cggccttggc gcgcgcatcg ttgaaggcgg cggcgatcag gtcttcgaga atgccctttt
      541 cgctcggctg catcaggctg tcgtcgatgc ccacaccgat cacccggccc ttggcggtgc
      601 aacggatcgt gaccagcccg ccacccgact tgccctcaac ctcgatctcg tcgagcttgc
      661 cctgcatttc gcccatctgt ttgctgatgg tctcggcggc ttgctgggcg gccttgatca
      721 tctcttccat cgacttcacg tctctatctc cagttgctgg ccttgccgac gacgcgcgcg
      781 tcggggcttt catcgatcag ttcggcgcgc gggaacgcgg ccatggtcgc ctcgaccaac
      841 gggtgggcca gggtttcagc ccggctcgcc tgtctggcgg cctcctccgc ttcgcgcagg
      901 ctgggagcgg catcgccctc ggcgcgggtt acctgccagc gctcgcccgt ggcgcgcagc
      961 agcgcatcgc gaatttcagg cgtgggatcg ccgggatagc cgggcgcgag gacataggtg
     1021 agctcggtcg gggcaaggtg catcacccgc acccaatcac gcatgacctg ggcaaggcgc
     1081 agctgccctg actggtcgac ctgatcgaca atccgggccc agtcgagcgc agtcggcgcc
     1141 ggcgccgcgg ccgcaaccgt gccaccttcc gccgaagccg gggtgccggc aacgaaactg
     1201 ccgctggccg ccatctcttc cagcttgcgc gccagcgttc cgggatcagg caggtcgctg
     1261 gcatgctgca cccgcagcag cgccatcttt gccgcaatca gcggatcggg cgccgatcgc
     1321 acctcgtcat gccccttgag catcagctgc cacaggcggt ggagctggcc tggcgatagc
     1381 gaaccagccc actggtcgat atccgcccgc tcttccgccg aagcgccatc ggcaccggac
     1441 cggccaagct gggcgaccgt gaccttgtga accagatcga gccccgcgcg catcagcgcc
     1501 agcggctcaa cccccagggc gaattgctga tcgaccatgt cgagcaaggc cggaccgtca
     1561 cccgccagca tggccgcgaa catgcgccgc agggccgact tgtcggccag gcccaacatg
     1621 tcctgcacac gcgcggcgat cacccgcccc tcgccgccaa ggtcggcatg ggcgatcgcc
     1681 tgatcgagga tcgaaaggcc atcacgcacc gaaccttcgg ccgccgcggc gatcatcgcc
     1741 agcgcctcgg gatcggcctc gacaccttcc ttatcgcaga tattggcgaa gtgctgggcc
     1801 agcaacgggg ccggaatccg ccgcaggtca aagcgctggc aacggctcag cacggtaacc
     1861 ggcagctttt caacctcggt ggtcgcgaac aggaacttca cgtgcggcgg cggctcttcc
     1921 agcgtcttca acagcgcatt gaaggcgttg cgcgagagca tgtggacttc gtcgatgatg
     1981 tagatcttgt agcgcgccga aactgcggca tagcggaccg cctcgatgat ttcgcgcaca
     2041 tcatcgaccc cggtgtggct ggcggcgtcc atttcgatga cgtcgatatg gcgcccttcg
     2101 gcgatcgcca cgcagggttc gcactggccg caagggtcaa tcgtcggtcc gccctgtccg
     2161 tccgggccaa tgcagttgag cgccttggca atcagccgcg cagtgctggt cttgcccacc
     2221 ccgcggatcc cggtcatcag gaaggcatgg gccagccggt cgcgcttgat cgcgttggcc
     2281 aaggtctgga ccatcgcatc ctggccgagc agttcggcaa aggtctgcga acggtactt
//
LOCUS       DASUDV010000422         6590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_941967,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000422 DASUDV010000000
VERSION     DASUDV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941967"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFV30_09175"
     CDS             <1..583
                     /locus_tag="VFV30_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848305.1"
                     /translation="PDAPVLEVGSGTHGIIFYFGTSNGVGIDPLADDYRALFPELHSR
                     ARTLAAPGEALPFPDASFDVVLCDNVVDHAENPRRILEEIARVSRPGALLYFEVNVHH
                     PVYHWAASLHAGWRALGVPFEITPFADHTYHFTRDGAKALLAGLPFRLVSESDTLAAE
                     AKRQRAMPLRRPSDLLKRLFVKNAAYEVVAVRK"
     gene            complement(599..1051)
                     /locus_tag="VFV30_09180"
     CDS             complement(599..1051)
                     /locus_tag="VFV30_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848306.1"
                     /translation="MLRQGYLALTCLFLACCSPPDDRNPELPLSAQDASRIELHVPNK
                     PLPPESTNVYYQDWEGMDSFQVVRFDLPLAKAKAIMTDLTGMAPAAVSPDGVRAMRKT
                     GLPFPSWWTIPEGAQGVTGARKDSCWPQSYVIVPTEGIARVYFTSSTC"
     gene            complement(1061..1525)
                     /locus_tag="VFV30_09185"
     CDS             complement(1061..1525)
                     /locus_tag="VFV30_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX4848307.1"
                     /translation="MANLDSIDRRLLAELQDEGRVTNVELAQRVGLTAPPCLRRVRAL
                     EESGVIRGYHADLDASKLGFAITVFAMVSLKSQAEDSLRQFEEAMRDLPEVRECHMLN
                     GEIDFILKIVSRDLQSFQEFLTSKLTPAPNVASVKTSLTIRTAKQMPGVPLE"
     gene            1731..3545
                     /locus_tag="VFV30_09190"
     CDS             1731..3545
                     /locus_tag="VFV30_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4848308.1"
                     /translation="MHFDDRLGTVLRLNASSEAMRRIQFRQLLDLLGVLPADARGEQI
                     DAAFVRLGELSKAIPAGLRTAVVRDVGLRLRSPRLVAALASGEPDVAQAALQRAELTR
                     EEWHDLIPALPPGARHALRRRSDLPRETVALLARLGVTDRGLPPAAAPAPDAPAAEAT
                     AKPETAPEPEPAAAAPAEEPQPQPEPEPEAEPVSALEPEPAASDELPTILPAQPAPGD
                     GAIGAIVKRIEAFRRARQVVEQRPANDSPRLPLGEEHVLQVPEQIRAFNFATDAEGRI
                     TWSDPGVAPMVTGIALPPAASDPAFAVRLRSHQPLTGQRLRLAGAPAIAGEWCLDAAP
                     WFDPLTGRYLGYRGRLRRPAERVPLVPPVTDSEADRLRQLLHELRTPVNAIQGFAEVI
                     QQQLFGPTPHDYRALAAGIAGDAARMLAAFEELERLARLDSGAMELDPGETDLAAMIE
                     ATASQLAAHTGQRGSGFAIRREVETLPVGLERIEVERIVWRLLATLAGVSAPGEMLKL
                     RLRERHGMARIDLALPKALADRPEESLFETAAGAIPQIITAGVFGVGFALRLARAEAR
                     AAGGDLIRRDGKLRLTLPGLTGDSGDHRQQQAGGADAV"
     gene            3616..4575
                     /locus_tag="VFV30_09195"
     CDS             3616..4575
                     /locus_tag="VFV30_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4848309.1"
                     /translation="MRFKPGFGQRFVLTVDTEEEFDWNRPIRREGHTLQTVARLARFQ
                     EFCEAQGVCPIYLIDYPIVTSPSAVAILRDAVAAGKAEVGVQLHPWVNPPFDEVVTET
                     NTFAGNLPPELETAKFRKLRDAIEKEIGKPPLIYRAGRYGVGPATAGILSGGGIAIDT
                     SVRARFDYSASGGPNFRDLPVRPWWIDRNAGLMELPLTTVYWGILRQFGPFLYPRMWR
                     VPRLRGGLARIGMLERIPLTPEGVNAQEALNAIDIAIDEGLPVLVFSFHSPSLAPGFT
                     PYVRNEDDLDAFYDWWRQVFAYLGKRGVAPTRVAELVDSVELA"
     gene            4618..4694
                     /locus_tag="VFV30_09200"
     tRNA            4618..4694
                     /locus_tag="VFV30_09200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4652..4654,aa:Ile,seq:cat)
     gene            4718..4794
                     /locus_tag="VFV30_09205"
     tRNA            4718..4794
                     /locus_tag="VFV30_09205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4752..4754,aa:Pro,seq:tgg)
     gene            4833..6059
                     /locus_tag="VFV30_09210"
     CDS             4833..6059
                     /locus_tag="VFV30_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4848310.1"
                     /translation="MEQADVVIVGAGHGGAQCALALRQAGFEGSVRVIGREPEIPYER
                     PPLSKEYLAREKTFERICIRPAQFWEDRQVTLDLGREVTRIDPAAHCLNLSDGTEIGY
                     GQLVWATGGDPRMLSCPGADLAGVHAVRTKADADALMAALDNGAKTAVVIGGGYIGLE
                     AAAVLTKFGVKVTLLEALPRVLARVAGEDLSRFYEAEHRAHGVDLRTEVAVEAIEGTD
                     GRASGVRLGDGTVLPADIVIVGIGIIPAVGPLLAAGAAGGNGVDVDELCRTSLPDVFA
                     IGDCAAHANGFAEGAVIRLESVQNANDMATTAAKAICGDPQPYRATPWFWSNQYDLKL
                     QTVGLSTGHDATVLRGDPAARSFSVLYLRKGKVIALDCVNAMKDYVQGRKLVEAGAQI
                     DPALLADAATPLKDML"
     gene            6074..6526
                     /locus_tag="VFV30_09215"
     CDS             6074..6526
                     /locus_tag="VFV30_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4848311.1"
                     /translation="MAGRYFEDWTVGDRIEHEIRRTVTETDNLLFSTMTHNPQPLHID
                     AEAARESEFGQILVNGTFTFALMVGLSVGDTTLGTLVANLGYDQLVMPKPVFIGDTMR
                     ATSEVAELKESKSRPGAGIVTFRHELINQRGEVVCLCLRSALIKRRAA"
BASE COUNT         1059 a         2265 c         2216 g         1050 t
ORIGIN      
        1 ccccgatgcg ccggtgcttg aagtcgggtc cggcacccac ggcatcatct tctatttcgg
       61 gaccagcaac ggcgtcggga tcgatccgct ggccgatgac taccgcgccc tgtttccaga
      121 gctgcacagc cgcgccagga cgctggccgc gccgggcgaa gcgctgccct ttcccgatgc
      181 cagcttcgat gtggtgctgt gcgacaacgt ggtcgaccat gccgaaaacc cgcgccgcat
      241 cctggaggag atcgcgcggg tcagccgccc cggggccctg ctctatttcg aagtcaacgt
      301 gcaccatccg gtttatcact gggccgccag cctccacgcc ggctggcgcg cgctgggcgt
      361 gccgttcgag atcacgccct tcgccgacca cacctatcac ttcacccggg acggcgcgaa
      421 agcgctgctg gccggcctgc ccttccgcct cgtcagcgaa agcgacaccc tggcggcgga
      481 agccaaacgg cagcgcgcca tgccgctgcg ccgcccgagc gacctgctca aacggctgtt
      541 cgtgaagaac gcggcttacg aggttgttgc ggtccggaag tgaaccggac cgccgggctc
      601 agcaggtcga actggtgaag tagacgcggg caatgccctc ggttggcacg atgacatagg
      661 attgcggcca gcagctgtct tttcgggcac cggtgacgcc ttgtgcgcct tccgggattg
      721 tccaccacga cgggaacggg agcccggtct ttcgcatcgc gcgaaccccg tccggtgaaa
      781 cagcggcggg agccatcccc gtcaaatcgg tcatgatggc tttggccttc gccagaggca
      841 ggtcaaaccg taccacctga aaggaatcca tcccctccca gtcctggtaa tagacgttgg
      901 tggattcagg cgggagcggc ttgttcggaa cgtggagttc gatccgggac gcgtcctgcg
      961 ctgagagcgg aagctcggga ttgcgatcat caggcgggct gcagcatgcc aggaacaggc
     1021 acgttaaggc cagatagccc tgccgcaaca tggccaggcc ctactccagc ggcaccccgg
     1081 gcatctgctt cgcggtgcgg atcgtcaggc tggtcttcac gctcgccacg ttgggcgccg
     1141 gggtcagctt gctggtcagg aattcctgga agctctgcag gtcgcggctg acgatcttca
     1201 ggatgaagtc gatctcgccg ttgagcatgt ggcattcgcg cacttcgggc agatcgcgca
     1261 tcgcctcttc gaactggcgc agcgaatcct cggcctggct tttcaggctg accatcgcga
     1321 atacggtgat cgcaaagccc agcttcgagg cgtccagatc ggcgtggtag ccgcggatca
     1381 cgcccgattc ctccagcgcg cgaacccggc gcaggcacgg cggcgccgtt aaccctaccc
     1441 gctgggccag ctcgacgttg gtcacccgcc cctcgtcctg gagctctgcc agcagccgcc
     1501 gatcgatcga atcgagattt gccatggccc taaccccttt gcggcaccct tggccgaaaa
     1561 cccgcgattt cggcaacttt tgccacaatc ggccgcggcc cgcgataata ttattgttta
     1621 tggcggcaac tgtcccgctt tgcaacgcac ctttcgcgaa ccgcccatgc tggggacaaa
     1681 actggtaaac gctctcgcac gcaccaaccg cctgccgtac cgggagcacc atgcatttcg
     1741 acgatcgcct tggaaccgtg ctgcgcctga acgccagcag cgaggcgatg cggcggatcc
     1801 agttccgcca gttgctggac ctgctgggcg ttctgccggc cgatgcccga ggcgagcaga
     1861 tcgacgccgc gttcgtccgg ctgggcgaac tgtccaaggc cattcccgcc ggtctgcgca
     1921 ccgcggtggt ccgcgatgtc gggctgcggc tgcgctcgcc ccggctggtc gcggcgctgg
     1981 ccagcggcga gcccgacgtg gcccaggccg cgctgcagcg ggccgagctg acccgggagg
     2041 aatggcacga cctgatcccg gccctgccgc ccggtgcccg acatgcgctt cgccgccgca
     2101 gcgacctgcc gcgcgagacc gtggccctgc tggcccggct gggcgtgacc gaccgcggcc
     2161 tgcccccggc cgccgctccg gctccggacg cgcccgcggc cgaagcaacc gccaagccgg
     2221 agaccgcgcc cgagcccgaa cccgcagctg ccgctcctgc cgaggagccc cagccccagc
     2281 ccgagcccga acccgaggcc gagccggtca gcgcgcttga accggaaccc gccgccagcg
     2341 acgagctccc cacgatcctg ccggcccagc cagcgcccgg cgacggggcg atcggagcca
     2401 ttgtcaagcg gatcgaggcc ttccgccgcg cccgccaggt ggtggagcag cgcccggcca
     2461 acgattcccc gcgcctgccg ctgggcgaag agcacgtgtt gcaggtgccc gaacagatcc
     2521 gcgccttcaa ctttgccacc gatgccgaag ggcggatcac ctggagcgat cccggcgtgg
     2581 ccccgatggt gaccggcatt gctctgccgc cggcagccag cgatccggcc tttgccgtgc
     2641 ggctgcgcag ccaccagccg ctgaccggcc agcggctgcg gctggccggg gccccggcca
     2701 tcgccggcga atggtgcctt gatgccgcgc cgtggttcga tccgctgact ggccgttatc
     2761 tgggctaccg cggccgcctg cgccgtccgg ccgagcgcgt gccgctggtg ccgccggtga
     2821 ccgacagcga ggccgaccgc ctccgccagc tgctgcacga actgcgcacg ccggtgaacg
     2881 cgatccaggg ctttgccgaa gtgatccagc agcaactgtt cggccccacc ccgcacgatt
     2941 accgcgcgct tgccgccggg attgccgggg atgcggcgcg gatgctggcc gcttttgaag
     3001 agctggagcg gctggccagg ctggattcgg gcgcgatgga actggatccc ggcgagaccg
     3061 atctggccgc gatgatcgag gccaccgcca gccagctggc cgcccacacc ggccagcgcg
     3121 gcagcggctt tgcgatccgc cgcgaggtgg aaaccctgcc ggtcgggctg gagcggatcg
     3181 aggtggagcg gatcgtctgg cggctgctcg cgaccctggc cggagtgagc gcgccgggcg
     3241 aaatgctgaa gctgcggctg cgcgaacggc acggcatggc ccggatcgac ctggccctgc
     3301 caaaagccct ggccgaccgg cccgaggaaa gcctgttcga gaccgccgcc ggggccatcc
     3361 cgcagatcat taccgcgggc gttttcgggg tcggctttgc cctgcggctg gcccgcgccg
     3421 aagcccgcgc ggcgggcggc gacctgatcc gccgcgatgg caagctgcgc ctgaccctgc
     3481 cgggcttgac cggggacagc ggggaccata ggcagcaaca agcaggcggg gcagacgccg
     3541 tttgacgggt ttcagcgggg ggactttgcc ttgtcggata agcggatcac cgacctgccg
     3601 gcaggatccg atttcgtgcg tttcaagccc ggcttcggcc agcgcttcgt cctgacggtc
     3661 gataccgagg aagagttcga ctggaaccgg ccgatccggc gcgaaggcca caccctgcag
     3721 actgtcgccc ggctggcccg gttccaggaa ttctgcgagg cgcagggcgt ctgcccgatc
     3781 tacctgatcg actatccgat cgtcacttcg ccttcggccg tggcgatcct gcgcgatgcc
     3841 gtggcagccg ggaaggcgga agtcggcgtc cagctgcacc cgtgggtcaa tcccccgttc
     3901 gacgaggtgg tgaccgagac caacaccttt gccggcaacc tgccgcccga actggagacg
     3961 gccaagttcc gcaagctgcg cgacgcgatc gaaaaggaaa tcggcaagcc gccgctgatc
     4021 taccgcgccg ggcgctatgg cgtggggccg gccaccgcgg ggatcctgtc tggcgggggc
     4081 atcgcgatcg acacctcggt ccgcgcccgg ttcgattaca gcgccagcgg cggccccaac
     4141 ttccgcgacc tgccggtccg cccgtggtgg atcgatcgca acgccgggct gatggagctg
     4201 ccgctgacca cggtctattg gggcatcctg cgccagttcg gcccgttcct ttacccccgg
     4261 atgtggcgcg tcccgcggct gcgcggcggg ctggcccgga tcggcatgct ggagcggatt
     4321 ccgctgacgc ccgaaggggt gaacgcacag gaagccctga acgcgatcga cattgccatc
     4381 gacgaaggcc tgccggtgct ggtcttctcg ttccattcgc cctcgctcgc gcccggcttc
     4441 acgccctatg tccgcaacga ggacgatctc gacgcgttct atgactggtg gcggcaggtc
     4501 tttgcctacc tcggcaagcg cggggttgcg ccgacccggg ttgccgaact ggtcgattcc
     4561 gtcgagcttg cctaagcccc cgtgccgagc taacgccgcg cggcattgcg cgcgcatggg
     4621 cctgtagctc agttggttag agctggccgc tcataacggc taggtcgcgg gttcgagtcc
     4681 tgccgggccc accagcgcgc aacggcggaa ccagggtcgg ggagtagcgc agcctggtag
     4741 cgcatctgct ttgggagcag agggtcgcag gttcgaatcc tgtctccccg accagtttcg
     4801 ccaagcccca tggaagccag aggacgagcg tgatggaaca ggcggatgtg gtgattgtcg
     4861 gggccggcca cgggggcgcg caatgcgcgc tcgcgctgcg ccaggccggg ttcgaaggat
     4921 cggtccgggt gatcggccgc gaaccggaaa tcccctatga gcgcccgccg ctgtccaagg
     4981 aatacctcgc ccgcgaaaag actttcgagc ggatctgtat ccgccccgcc cagttctggg
     5041 aggaccgcca ggtcacgctc gacctgggcc gcgaagtgac ccggatcgat ccggctgccc
     5101 actgtctgaa cctgagcgac gggaccgaaa tcggctatgg ccagctggtc tgggcgaccg
     5161 gcggggatcc gcggatgctg agctgccccg gggccgacct ggccggggtt cacgccgtac
     5221 gcaccaaggc cgatgccgat gcgctgatgg ccgcgctgga caatggcgcg aagacggcgg
     5281 tggtgatcgg cggcggctat atcgggctgg aagccgcggc cgtgctgacc aagttcgggg
     5341 tcaaggtgac cctgctggaa gccctgcccc gcgtcctcgc ccgggtcgca ggagaggacc
     5401 tgtcccggtt ctacgaagcc gagcaccgcg cccacggcgt ggatctgcgc accgaagtgg
     5461 cggtcgaagc gatcgagggc acggacggcc gcgccagcgg cgtccggctg ggcgacggga
     5521 ccgtcctgcc ggccgacatc gtgattgtcg ggatcggcat catccccgcc gtgggtccgc
     5581 tgctggctgc cggggcggca ggcggcaacg gggtcgatgt ggatgagctg tgccgcacct
     5641 cgctgcccga tgtttttgcc atcggagact gcgccgctca cgccaacggc tttgccgaag
     5701 gcgcggtgat ccggctcgaa tcggtccaga acgccaatga catggcgacc accgcggcca
     5761 aggcgatctg cggcgatccc cagccctacc gcgcgacgcc gtggttctgg tccaaccagt
     5821 atgacctgaa gctgcaaacg gtcggccttt cgaccggcca tgacgccacg gtcctgcgcg
     5881 gcgatccggc cgcgcggtcg ttctcggtcc tctatctgcg caagggcaag gtgattgcgc
     5941 tcgattgcgt caacgcgatg aaggactacg ttcagggccg caaactggtt gaggccgggg
     6001 cgcagatcga tccggcgttg ctggccgatg ccgccacgcc gctcaaggac atgctctaac
     6061 agggacaccc gccatggccg gacgctattt cgaagactgg acggtgggag accggatcga
     6121 gcacgagatc cgccggaccg tgaccgagac cgacaacctg ctgttctcga ccatgaccca
     6181 caacccccag ccgttgcaca tcgatgccga ggcagcgcgg gaaagcgaat tcggccagat
     6241 cctggtcaac ggcactttca cattcgcgct gatggttggc ctgtcggtcg gcgacaccac
     6301 gctgggcacg ctggttgcga atctcggcta tgaccagctg gtgatgccga agccggtgtt
     6361 catcggcgat accatgcgtg cgaccagcga agtggcggag ctgaaggaat cgaagagccg
     6421 cccgggcgcc ggcatcgtca ccttccggca cgaactgatc aaccagcgtg gcgaagtcgt
     6481 ctgcctctgt ctgcgctcgg ccctgatcaa gcgccgcgcc gcctgaaacg ggcagattgt
     6541 cgcgctacgg ccttgcaaag cgacaaacaa ttacacttcg cggcgtgaca
//
LOCUS       DASUDV010000423         5642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_367849,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000423 DASUDV010000000
VERSION     DASUDV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367849"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1258)
                     /locus_tag="VFV30_09220"
     CDS             complement(23..1258)
                     /locus_tag="VFV30_09220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848312.1"
                     /translation="MASIAQGSRISAARLNWLVILIGLSVLLNYVDRGAIGIAAPLMK
                     AELDLSATGFGTAVSAFFWVYAPLCLFVGWLCDRFCVYRMFAAGIALWAVATLLTGFV
                     HGIAALIVLRLILGLGESIAFPGSSKIFAAEVPAARRGYANAMVAAALAFGPAVGTFS
                     GGLILEELGWRPIFWIFGAVTLLWLVPWHYVSAPLRTHSVAVPVADPVPLKTIMRVPA
                     VWLMGVGHFCTNYGFYFLLTWLPLYLVKSRGFSITDMTLLTTLGFAVQGVMALVVGQV
                     SDKWVAGGADEARVRRRMMIAGQALAAVAIGAVFFADGFAQVGACLVLAGFASALTST
                     NLFAIGQMFAGPRAAGVWIGLQNAIGNAAGIIGPVLTGVMIDRLGGYGWAFALTALVP
                     ALGALWWWKVVPPIRQFES"
     gene            complement(1413..2627)
                     /locus_tag="VFV30_09225"
     CDS             complement(1413..2627)
                     /locus_tag="VFV30_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HEX4848313.1"
                     /translation="MEWIPTRAAGLERLAQFLPSAGRAYAERRNVDLGPQDRSNVSAL
                     SPWLRRRLITEEEVVAAVLTRHSAAAAEKFIQEVAWRTYWKGWLELRPAMLERFDAER
                     IALKAELARDGNLARRFARATDGATGIACFDAWVEELRELGWLHNHARMWFASIWIFT
                     LGLPWQLGADFFYKHLLDADPASNTLSWRWVAGLHTPGKHYLARAENIARNTGGRFAP
                     HGQLNEAALPLTEDAPPLPVTPLAPARRHDGGPCALLLTEEDLHPESWDLAGEIKAIA
                     VLPSASVAELDSPTAKFSAGALADASARVETYFGVSCHTVAAADLPGWLAGHGLMAMV
                     TAQAPVGLIAWQLRTVKARLAADGARLVELRRAWDELLWPLATAGFFKLKTRLPDVIA
                     RLGLDPAAPRLL"
     gene            complement(2685..4244)
                     /gene="guaA"
                     /locus_tag="VFV30_09230"
     CDS             complement(2685..4244)
                     /gene="guaA"
                     /locus_tag="VFV30_09230"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX4848314.1"
                     /translation="MSIQPSDSILIVDFGSQVTQLIARRVREAGVYSEIAPFSSAAAA
                     FQRMKPKGIILSGGPASVLDENGPRIPEEILESGLPIMAICYGQQALMHQLGGEVVQG
                     DSGEFGRAFIEVSDRCVLFDGLWAEGETHQVWMSHGDKVTALAPGFRPVASSPGAPYA
                     VIANDEKRIYAMQFHPEVVHTPDGAKLYKNFVRHVCGLAGDWTMAEFRKTKIEEIRTQ
                     VGTGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLMRLGEAEQVVSLFRGHYN
                     IPLVHKDVSALFLNGLAGVTDPEAKRKFIGKTFIDVFEEEARQVGGADFLAQGTLYPD
                     VIESVSFTGGPSVTIKSHHNVGGLPERMNMQLVEPLRELFKDEVRELGRELGLPEIFV
                     GRHPFPGPGLAIRIPGEVTRERCDILRKADAIYLEEIRNAGLYDAIWQAFAVLLPVKT
                     VGVMGDGRTYDSVCALRAVTSTDGMTADIYPFDAAFLSRVATRIINEVKGINRVVYDY
                     TSKPPGTIEWE"
     gene            4365..5147
                     /locus_tag="VFV30_09235"
     CDS             4365..5147
                     /locus_tag="VFV30_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX4848315.1"
                     /translation="MTEAPTTPYPRFPVARDLALADLGAALAAGWRDFLAQPLYGLFF
                     AAIYVAGGLGVSYALLTKGAAAWLIPLAAGFPLIAPFTAVGLYEVSRRREGGLPLSWG
                     KVLGALRGHGDDQILLMGGFIFVGFTFWIILAHGIFAIFAAQLGPGSESLAMLRTPTG
                     LAMLAVGSAVGAMVSFVFYAITVISLPMLVDRDVDFLTAIITSLRAIQANRAALLAWA
                     TFIALVLFAAMLPAFAGLLVALPVLGHATWHLYRRTVSETPA"
     gene            complement(5144..>5642)
                     /locus_tag="VFV30_09240"
     CDS             complement(5144..>5642)
                     /locus_tag="VFV30_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-coumarate--CoA ligase"
                     /protein_id="HEX4848316.1"
                     /translation="GTPGELVGKSPTMMLGYKNQPEKTREGYWIDPEDGSVWQRMGDV
                     GRVDADGFVELVGRTKDVIISGGFNIFPIDLEGELAKDERVVEAAVVGMPSEQWGETP
                     VGFVVLKPGVGEDALEDIRTIANSRLGKTQRVAELYAIDEMPRSHIGKLLKTDLRELV
                     PAKPR"
BASE COUNT          963 a         1929 c         1838 g          912 t
ORIGIN      
        1 aatcatagtc tgaggacgct gatcagctct cgaactgccg gatcggcggc acgaccttcc
       61 accaccacag cgcgcccagc gccgggacca gcgcggtcag cgcgaaggcc cagccatagc
      121 cgccgagccg atcgatcatc accccggtca gcaccggacc gatgatgccg gcggcgttgc
      181 cgatcgcgtt ctggaggccg atccacaccc ctgcggcgcg cgggccggca aacatctgcc
      241 cgatcgcgaa caggttggtg gaggttagcg cgctggcgaa cccggccagc accaggcagg
      301 cgccgacctg ggcaaagcca tcggcaaaga ataccgcgcc gatcgccaca gcggccagcg
      361 cctgcccggc gatcatcatc cgccgccgga cccgcgcctc atcggcgccg cccgcgaccc
      421 acttgtccga gacctgaccc accaccagcg ccatcacccc ctggacggca aaacccagcg
      481 tggtcagcag cgtcatgtcg gtgatcgaga atccgcgcga tttgaccagg tacagcggca
      541 gccaggtcag caggaagtag aacccgtaat tggtgcagaa atgcccgacg cccatcagcc
      601 agaccgcggg cacgcgcatg attgtcttga gtggcaccgg atcggccaca ggcaccgcca
      661 cgctgtgcgt gcgcaaaggt gccgagacat aatgccaggg caccagccag agcagcgtga
      721 ccgcgccgaa aatccagaag atcggccgcc agcccagctc ttcaaggatc agaccgccgc
      781 tgaacgtgcc gactgccggg ccaaaggcga gcgccgccgc caccatcgca ttggcatagc
      841 cgcgccgggc ggcaggaacc tctgcggcaa agatcttcga gctgcccgga aaggcgatgc
      901 tttcccccag ccccaggatc agccgcagca cgatcagcgc cgcgatcccg tggacgaagc
      961 cggtcagcag cgtcgccacg gcccacagcg caatgccggc ggcgaacatg cggtagacgc
     1021 agaaccggtc acacagccag cccacgaaca ggcagagcgg cgcatagacc cagaagaagg
     1081 ccgagaccgc cgtgccgaaa ccggtggcgg aaaggtccag ctccgccttc attagcggcg
     1141 cggcaatgcc aatggcgccg cgatcgacat agttgagcag gaccgacagg ccgatcagga
     1201 tcaccagcca gttgagccgc gccgcgctga tccggctgcc ttgtgcaatg cttgccatgt
     1261 gatcaccccc cggttgcgcc gcaggctatc agcaatcccg ccgatggcga ggggaaatgc
     1321 agcgaggcct cagccgtaga accgcgcgcg gaagcggtcg gtgtggcggg ccaggttggc
     1381 gcggccctat gccgacttgc gacgcctccg gtttagagca gccggggcgc cgccggatca
     1441 agccccagcc gcgcgatcac gtcgggcagt ctggtcttga gcttgaagaa acccgcagtc
     1501 gccagtggcc agagcaattc atcccacgca cgacggagct caaccagccg cgcgccatcg
     1561 gccgcaagcc gcgccttgac cgtgcggagt tgccaggcga tcaggccaac cggggcctgt
     1621 gccgtcacca tggccatcag cccatgtccg gccagccagc ctggcaaatc agcagcggca
     1681 acggtgtggc acgaaacgcc gaagtaagtc tcgacgcgtg cggacgcatc ggccaacgct
     1741 ccagctgaga acttcgcggt agggctgtcc aattcggcca cgctggcgct tggcagcacc
     1801 gcaatcgcct tgatctcgcc cgccaagtcc caactttcgg gatgcaggtc ttcctcggtc
     1861 agcagcagcg cgcaggggcc gccatcgtgc cgccgggccg gggccagcgg cgtgacaggc
     1921 agcggcggcg catcctccgt cagcggcagc gccgcttcat tgagctggcc atggggtgcg
     1981 aaccgcccac ctgtattgcg cgcgatattc tcggcccggg ccaggtaatg cttgcccggc
     2041 gtatgcagcc ccgcgaccca gcgccacgat agcgtgttgg aggccggatc ggcatcgagc
     2101 aggtgcttgt agaaaaagtc cgcccccagc tgccacggca ggcccagggt gaagatccat
     2161 atgctggcga accacattcg cgcatggttg tgcagccagc ccagctcgcg cagttcctcc
     2221 acccaggcat cgaagcaggc aatgccggtt gcgccatcgg tcgcacgagc gaagcggcgg
     2281 gccagattgc catcgcgggc gagttcggcc ttgagcgcga tccgctcggc atcgaaccgc
     2341 tcaagcatgg caggccgcag ttcgagccag cccttccagt aggtccgcca ggccacttcc
     2401 tggatgaact tttcggccgc tgccgcgctg tgccgcgtca gcactgccgc caccacctct
     2461 tcctcggtga tcagccgccg ccgcagccag ggcgagagcg ccgagacgtt cgagcggtcc
     2521 tgcggcccca gatcgacatt gcggcgttcg gcataggcgc ggccggcact gggcaggaac
     2581 tgcgccagcc gttcaaggcc cgcggcgcgg gtcgggatcc attccatgcc ctgccgttaa
     2641 gtgcgggacg ggcaggcgtc agcccggact ttggaccggc aggctcactc ccattcgatc
     2701 gtcccgggag gctttgacgt atagtcatag accacgcggt tgatgccctt gacctcgttg
     2761 ataatgcggg tcgcgacgcg gctgaggaag gcggcgtcga agggatagat atccgccgtc
     2821 atcccatcgg tgctggtcac ggcgcgcagc gcgcagaccg agtcataggt ccggccatcg
     2881 cccatcacgc cgacggtctt gaccggcagc agcacggcga aggcctgcca gatcgcgtca
     2941 tagaggccgg cgttgcggat ctcctcaagg tagattgcat cggccttgcg cagaatgtcg
     3001 cagcgctcgc gggtcacttc gccggggatg cggatcgcga ggcccggccc cgggaaggga
     3061 tggcggccaa cgaagatctc gggcaggccg agctcgcggc ccagctcgcg aacctcgtcc
     3121 ttgaacagtt cgcgcagcgg ttcgaccagc tgcatgttca tgcgttcggg caggccgccg
     3181 acgttgtggt ggctcttgat cgtgacgctc ggcccaccgg tgaaggagac cgattcgatc
     3241 acgtccggat agagcgtgcc ctgggcgagg aagtccgccc cgccgacttg cctggcctct
     3301 tcctcgaaca cgtcgatgaa ggtcttgccg atgaacttgc gcttggcttc cgggtcagtc
     3361 acgcccgcca ggccattgag gaacagggcc gagacatcct tgtggaccaa cgggatgttg
     3421 taatggccgc ggaacagcga aacgacctgc tcggcctcgc ccagccgcat caggccatga
     3481 tcgacgaaga cgcaggtcag ctgatcgccg atcgcctcgt ggatcagcac cgccgcgacc
     3541 gccgaatcga ccccgcccga caggccgcag atcaccttgc cggtgccgac ctgggtgcgg
     3601 atttcctcga tcttggtctt gcggaactcg gccatggtcc agtcaccggc gaggccgcag
     3661 acgtggcgga cgaagttctt gtagagcttg gccccgtccg gcgtgtggac cacttcgggg
     3721 tggaactgca tcgcgtagat gcgcttctcg tcattcgcga tcaccgcata gggcgcgccg
     3781 ggcgagctgg cgaccgggcg gaagccgggg gcgagcgcgg tcaccttgtc gccgtggctc
     3841 atccagacct ggtgcgtctc gccttcggcc cagaggccat cgaacagcac gcagcgatcc
     3901 gacacctcga taaaggcgcg gccgaattcg ccgctatcgc cctgcaccac ttcaccgccc
     3961 agctggtgca tcagcgcctg ctggccatag cagatcgcca tgatcggcag gccgctctcc
     4021 aggatctctt ccgggatgcg cgggccgttt tcatcgagca ccgaggccgg cccgccggag
     4081 aggatgatcc ccttgggctt catccgctgg aacgcggcgg cggcgctgct gaacggggcg
     4141 atttcggaat agaccccggc ctcgcgcacg cggcgggcga tcagctgggt aacctggctg
     4201 ccgaaatcga cgatcaggat cgaatctgaa ggctggatgc tcatggctgg ccgataagga
     4261 agccgggcca aggtggcaag cgcggcgcgc ctgctgccaa cacttgatac gggtcaatga
     4321 tcgcgcgcac cgggctgtca ttctgcgctg ggggagtaga agcgatgaca gaggcaccaa
     4381 ccacacccta tccgcgcttt ccggtggcgc gcgacctggc gctggccgat ctgggcgcgg
     4441 cactggcggc gggctggcgc gatttcctcg cccagccgct ttacggcctg ttctttgccg
     4501 cgatctatgt cgctggcggc cttggggtca gctatgcgct cctgaccaaa ggcgcggcgg
     4561 cctggctgat cccgctggcc gcgggttttc cgctgatcgc gccgttcacg gcggtcgggc
     4621 tctacgaggt cagccgccgg cgcgagggcg ggctgccgct atcctggggc aaggtgctgg
     4681 gcgcgctgcg cgggcatggc gacgatcaga tcctgctgat gggcgggttc atcttcgtcg
     4741 gcttcacctt ctggatcatt ctggcccacg ggatcttcgc gatctttgcc gcgcaacttg
     4801 gcccgggcag cgaatcgctg gccatgctgc gcacgccgac gggccttgcc atgctggccg
     4861 ttggcagcgc ggtgggggcc atggtttcgt tcgtgttcta tgcgatcacc gtgatcagcc
     4921 tgccgatgct ggtcgatcgc gacgtcgatt tcctcaccgc gatcatcacc agcctgcgcg
     4981 cgatccaggc caaccgcgcg gcgctgctgg cctgggccac gttcatcgcg ctggtgctgt
     5041 tcgcggcgat gctcccggcc tttgccggcc tgctggtggc actgcccgtg ctgggccacg
     5101 ccacctggca tctctaccgg cggacggtga gcgagacccc ggcctagcgc ggcttcgccg
     5161 gcaccagttc gcgcaggtcg gtcttgagca gtttgccgat gtggctgcgc ggcatctcgt
     5221 caatcgcata gagctcggcg acgcgctggg tcttgccgag gcggctgttg gcgatggtgc
     5281 ggatgtcctc cagcgcgtcc tcacccacgc cgggtttcag cacgacgaag ccgactgggg
     5341 tttcgcccca ctgttcgctc ggcatgccca ccaccgcggc ttcgaccacg cgctcgtcct
     5401 tggccagttc gccttcaagg tcgatcggga agatgttgaa cccgcccgag atgatcacgt
     5461 ccttggtccg cccgaccagt tcgacgaagc catcggcatc gacccggcca acatcgccca
     5521 tccgctgcca gaccgagccg tcctcgggat cgatccagta gccctcgcgg gtcttttccg
     5581 gctggttctt gtagcccagc atcatcgtgg gcgatttgcc gaccagttcg cccggggtgc
     5641 cc
//
LOCUS       DASUDV010000424         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1582714,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000424 DASUDV010000000
VERSION     DASUDV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582714"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFV30_09245"
     CDS             <1..940
                     /locus_tag="VFV30_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4848317.1"
                     /translation="YAGHRLVEEALKEFYAMDHAMVFSTGYQANLGIISTIAGKGDYI
                     VLDIDSHASIWDGCKMGDAEVVPFKHNDIEAMDKRLGRIPEGAGKLVVLEGVYSMLGD
                     IAPLKEMIAVAKKHGAMVLVDEAHSMGFIGPNGRGVAEDQGVLEDVDFVIGTFSKSVG
                     TVGGFCVSNHPKFEIMRLVCRPYVFTASLPPSVVHTAATSIRKLMHAGNKRAHLWENS
                     KVLHKGLTEAGFQLGTKEPESAIIAVIMPDLERGAAMWEALLHEGLYVNLARPPATPA
                     NMTLLRCSLCAEHTAEQVQTIIGMFKRAGQAVGILG"
     gene            933..1730
                     /locus_tag="VFV30_09250"
     CDS             933..1730
                     /locus_tag="VFV30_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX4848318.1"
                     /translation="MARWALAALALLAGAAPAWAAERMNPYPPPGMAENACPVSRDGL
                     WAQDERVRASDWAWLCRYREDNARIDPGNPPRVVFIGDSITENWVGLDPAVFGPGRLG
                     RGISGQTSPQLLVRFWQDVVALKPRVVHIMVGTNDIAGNTGPTTPEAYKNAIRAMVDL
                     AEANGIVVILASIPPADRFGWSQAIRPAPWIATLNTWLKSYAAERGAVYADYHAVLTG
                     PNGELRPEFGPDGVHPNAAGYAAMRPVAEAAIEQAEAIAAGRDAAQG"
     gene            1734..2495
                     /locus_tag="VFV30_09255"
     CDS             1734..2495
                     /locus_tag="VFV30_09255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4848319.1"
                     /translation="MGGRLSGKRAVITGAAQGIGKAIAARFAAEGAELLLADIDAERL
                     AATAAELGQEGFVCDVARKAEIDRLFARVAERWAGLDLLVNNAGVTHAAELDALEEED
                     FDRVFAINLKSALWGTQAAARLMPPGSAVVNMSSVNAVLAIPNQIPYTVSKAALKQLT
                     NVTALALAPKGIRVNAIGPGSIMTDMLRGIMNDAAAEERILSRTPLGRCGEPEEVAAV
                     AVFLASGESSYLTGQTIYPDGGRLGLNYTVPVTRG"
     gene            complement(2482..>2968)
                     /locus_tag="VFV30_09260"
     CDS             complement(2482..>2968)
                     /locus_tag="VFV30_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX4848320.1"
                     /translation="RYMSVRLVYRNNLFYYTMMPGIWCSAVLVYLGLGWVYAGYIVIK
                     LAVISGAHSDVAWDRPLYRIKALSPLMWLVERTISTPSTHHMHHGRHADDPAVNYKGN
                     YGNLLFFWDVLFGTAKITRAYPQSYGVENLPPAHFGQQLAWPLVPENTEMEGEAQPSR
                     G"
BASE COUNT          512 a         1004 c          999 g          453 t
ORIGIN      
        1 ctatgccggc caccgcctgg tcgaagaggc gctcaaggaa ttctacgcca tggatcacgc
       61 catggtgttc tccaccggct atcaggccaa cctgggcatc atcagcacga tcgccggcaa
      121 gggcgattac atcgtgctgg atatcgacag ccacgccagc atctgggacg gctgcaagat
      181 gggcgatgcc gaagtggtgc ccttcaagca caacgacatc gaggcgatgg acaagcgcct
      241 cggccggatc cccgaaggcg ccggcaagct ggtggtgctg gaaggcgtct attcgatgct
      301 gggcgatatc gccccgctga aggagatgat cgcggttgcc aagaagcacg gcgcgatggt
      361 gctggtcgac gaggcgcact cgatgggctt catcggcccc aacgggcgcg gcgtggccga
      421 ggaccagggt gtgctggagg atgtcgactt cgtgatcggg accttctcca agtcggtcgg
      481 cacggtcggc ggcttctgcg tgtcgaacca ccccaagttc gagatcatgc ggctggtctg
      541 ccgcccctat gtcttcaccg ccagcctgcc gcccagcgtg gtccacaccg cggccacctc
      601 gatccgcaag ctgatgcacg ccggcaacaa gcgcgcccac ctgtgggaga attccaaggt
      661 cctccacaag ggcctgaccg aggccgggtt ccagctgggc accaaggagc cggaaagcgc
      721 gatcatcgcg gtgatcatgc ccgatctgga gcgcggcgcg gcgatgtggg aagcgctgct
      781 gcacgaaggg ctctatgtga acctggcccg cccgccggcc accccggcaa acatgaccct
      841 gctgcgctgc tcgctctgcg ccgagcatac cgccgagcag gtccagacca tcatcggcat
      901 gttcaagcgc gccggacagg cggtcgggat ccttggctag gtgggcgctg gccgctcttg
      961 ccttgctggc cggggccgcc ccggcctggg cggctgagcg catgaacccc tatcccccgc
     1021 caggcatggc cgaaaacgcc tgccccgtca gccgcgacgg gctgtgggcg caggacgagc
     1081 gggtccgcgc cagcgactgg gcctggctgt gccgctaccg cgaggacaac gcccggatcg
     1141 atccgggcaa tcccccgcgg gtcgtcttca tcggcgattc gatcaccgaa aactgggtcg
     1201 gtctcgatcc ggcggtgttc ggccccggcc ggctgggccg cggcatttcc gggcagacca
     1261 gcccgcagct gctggtccgt ttctggcagg acgtggtcgc gctgaagccg cgggtggtcc
     1321 acatcatggt cggcaccaac gacatcgccg gcaacaccgg gccgaccacg ccggaagcct
     1381 acaagaacgc gatccgggcg atggtcgatc tggccgaggc caacggcatc gtcgtaatcc
     1441 tggccagcat cccgccggcc gaccgctttg gctggagcca ggcgatccgg cccgcgccgt
     1501 ggatcgccac gctgaacacc tggctgaaga gctatgccgc cgagcgcggc gcggtctatg
     1561 ccgattacca cgccgtgctg accggcccga acggcgagct gcgccccgaa ttcggccccg
     1621 acggggtcca ccccaacgcc gccggctatg ccgcgatgcg cccggtcgcc gaagcggcga
     1681 tcgaacaggc cgaagcgatc gccgccggcc gcgacgcggc gcagggctga gcggtgggcg
     1741 ggcggctttc cggcaagcgc gcggtcatca ccggcgcggc gcaagggatc ggcaaggcga
     1801 ttgccgcgcg cttcgccgcg gaaggcgcag agctgctgct ggccgatatc gacgccgaac
     1861 ggctcgccgc caccgcggcc gaactgggcc aggaagggtt cgtctgcgat gtcgcccgca
     1921 aggccgagat cgaccgcctg tttgcccggg tggcggagcg ctgggccggg ctggacctgc
     1981 tggtcaacaa cgccggggtg actcacgccg ccgagctgga tgcgctggag gaagaggatt
     2041 tcgaccgggt cttcgcgatc aacctcaagt ccgcgctctg gggcacccag gccgcggccc
     2101 ggctgatgcc cccgggatcg gcggtcgtga acatgagcag cgtcaacgcg gttctggcga
     2161 tccccaacca gattccctac actgtatcca aagcggcact aaagcagcta accaatgtaa
     2221 ccgcattggc acttgcgccc aaaggcatcc gcgtgaacgc gatcggcccc ggctcgatca
     2281 tgaccgacat gctgcgcggg atcatgaacg atgccgccgc cgaagaacgg atcctttcgc
     2341 gcaccccgct gggccgctgc ggcgagcccg aggaagtggc ggcggtggcg gtgttcctgg
     2401 ccagcggcga aagcagctat ctgaccggcc agaccatcta ccccgacggc ggccgcctcg
     2461 gcctcaacta cacggtcccc gtcacccgcg gctaggctgt gcctcgccct ccatctccgt
     2521 gttttccggc accagcggcc aggccagctg ctggccgaaa tgggccggcg gcaggttttc
     2581 caccccgtag ctttgcggat aggcgcgggt gatcttggcc gtgccgaaca ggacatccca
     2641 gaagaacagc aggttgccgt agttgccctt gtaattgacc gccggatcat cggcgtggcg
     2701 gccgtggtgc atgtggtggg tcgagggggt ggaaatggtc cgctcgacca gccacatcag
     2761 cggggacagg gccttgatcc ggtagagcgg ccggtcccag gcgacgtcgc tgtgcgcccc
     2821 gctgatcacc gccagcttga tcacgatata gccggcatag acccagccga gcccgaggta
     2881 gaccagcact gccgagcacc agatccctgg catcatcgta taatagaaca ggttgttccg
     2941 gtaaacgagc cggacgctca tgtagcgg
//
LOCUS       DASUDV010000425         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1560688,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000425 DASUDV010000000
VERSION     DASUDV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560688"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1023)
                     /gene="galU"
                     /locus_tag="VFV30_09265"
     CDS             complement(127..1023)
                     /gene="galU"
                     /locus_tag="VFV30_09265"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX4848321.1"
                     /translation="MTESSHRKPIRKAVFPVAGLGTRFLPATKAIPKEMLPIIDRPLI
                     QYAVDEAREAGIEQLIFVTGRGKTAIVEHFDMAFELETTMKGRDKALDALEPTRIQPG
                     NLVAVRQQVPLGLGHAIWCARAIVGDEPFAIFLPDELMIANKGGAGCMKQMVEAYNAV
                     GGNLISVLEVPEAEVSSYGVIAPGKQDGALTEVLGLVEKPKREEAPSNLIISGRYILQ
                     PEVMRVLESQGKGAGGEIQLTDAMARMIGQQPFHAVTFAGKRYDCGSKTGFVEATLAI
                     ALEREDMADDVRAMAKRLLAEG"
     gene            1130..>2295
                     /gene="murA"
                     /locus_tag="VFV30_09270"
     CDS             1130..>2295
                     /gene="murA"
                     /locus_tag="VFV30_09270"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4848322.1"
                     /translation="MDKIIIEGGKALSGTIPISGAKNSALTLLPCALLTEEPLTLRNL
                     PRLADIDGFQHLLNQFGISTSIAGNRPGDFGRVMTLEAPRITSTVAPYELVRKMRASI
                     LVLGPMLARMGEATVSLPGGCAIGNRPIDLHLKALEAMGATIELASGYVKAIAPDGGL
                     PGGRYSFPVVSVGATENAVMAAVLCTGKTVLHNAAREPEIVDLCKLLVAMGAQIEGIG
                     TSDLTIHGVPRLHGATYRVMSDRIEAGSYACAAAITGGEVFLQHAVAEEMDATLQALR
                     DAGVHVEPRAGGIHVAAQGKMKAVTLATAPYPGFATDMQAQLMAMLCLAEGSSVLTET
                     IFENRYMHVPELNRMGAHIETKGRTAVVHGVEKLTGAEVMATDLRASMSLVIAGL"
BASE COUNT          360 a          796 c          753 g          386 t
ORIGIN      
        1 cgtgaactca ccgtgccgga cgccgatcac ttctcgatcc tgtacgagct cagccgcgaa
       61 ggcggcgtgc tgcgcgccgc gatcggcgat ctgctggaag cggtgccggc cggctagggc
      121 cgcgcctcag ccctcggcca ggagccgctt cgccatggcc cgcacgtcgt cggccatgtc
      181 ctcgcgctcc agtgcgatgg ccagggtcgc ctcgacaaag ccagtcttgc tgccgcagtc
      241 atagcgcttg ccggcaaagg tgaccgcgtg gaacggctgc tggccgatca tccgcgccat
      301 cgcgtcggtc agctggattt ccccgccggc gcccttgccc tggctttcca gcacccgcat
      361 cacttcgggc tgcaggatat agcggcccga gatgatcaag ttcgacgggg cctcttcgcg
      421 cttgggcttt tcgaccaggc ccagcacttc ggtcagcgcg ccgtcctgct tgccgggcgc
      481 gatcacgccg tagctggaga cttccgcttc cggcacttcc agcaccgaga tcaggttgcc
      541 gccgaccgcg ttataggcct cgaccatctg cttcatgcag ccggcgccgc ccttgttggc
      601 gatcatcagc tcgtcgggca ggaagatcgc gaacggctcg tcccccacga tcgcccgggc
      661 gcaccagatc gcgtggccca ggcccagcgg cacctgctgg cgcacggcga ccaggttgcc
      721 gggctggatc cgggtcggct ccagcgcgtc gagcgccttg tcccgcccct tcatggtggt
      781 ttccagttcg aaggccatgt cgaaatgctc gacaatcgcg gtcttgcccc ggccggtcac
      841 gaagatcagc tgttcgatcc ccgcctcgcg cgcctcgtcg accgcgtact ggatcagcgg
      901 ccggtcgatg atcggcagca tttccttggg aatcgccttg gtcgcgggga gaaagcgggt
      961 gccaagcccg gccaccggga aaacggcctt ccggatcggt ttgcgatggg aactctcggt
     1021 catgctgggc ctttggcaag tgcgtgttgc agcggcgtta aagcggtttc caccgatgcg
     1081 cgcaacgccc ggttgccgcg ggcgcgattc ccgctaaatc gtgaagccta tggacaagat
     1141 catcatcgag ggcggcaagg ccctttccgg caccattccg atctctggcg cgaagaattc
     1201 ggcgctgacc ctgctgcctt gcgcgctttt gaccgaagag ccgctgaccc tgcgcaacct
     1261 gccgcggctg gccgatatcg atgggttcca gcacctgctc aaccagttcg gcatctcgac
     1321 cagcatcgcc ggcaaccgcc cgggcgattt tggccgggtg atgacgctgg aagcgccgcg
     1381 gatcaccagc acggttgcac cctatgaact ggtccgcaag atgcgcgcct cgatcctggt
     1441 gctgggcccg atgctggccc ggatgggcga ggcgaccgtt tcgctgcccg gcggctgcgc
     1501 aatcggcaac cgcccgatcg acctgcacct gaaagcgctg gaagcgatgg gcgcgacgat
     1561 cgagctggcc tcgggctatg tgaaggcgat cgcccccgat ggcggcctgc ccggcgggcg
     1621 ctattccttc ccggtggtct cggtcggcgc gaccgagaac gcggtgatgg ccgccgtgct
     1681 ctgcaccggc aagaccgtgc tgcacaacgc cgcgcgcgag ccggaaatcg tcgacctgtg
     1741 caagctgctg gtggcgatgg gtgcccagat cgaagggatc ggcaccagcg acctgacgat
     1801 ccacggcgtg ccccgcctgc acggcgcgac ctaccgcgtg atgagcgacc ggatcgaggc
     1861 cggctcctat gcctgcgccg cggcgatcac cgggggcgaa gtgttcctgc agcacgccgt
     1921 ggccgaggaa atggacgcca ccctgcaggc gctgcgcgat gccggggtcc atgtcgagcc
     1981 gcgcgccggc gggatccatg tcgccgccca gggcaagatg aaggccgtca cccttgccac
     2041 cgcgccctac cccggctttg ccaccgacat gcaggcccag ctgatggcga tgctgtgcct
     2101 ggccgaaggc agctcggtgc tgaccgagac gatcttcgaa aaccgctaca tgcacgtccc
     2161 cgaactcaac cggatgggcg cgcacatcga aaccaagggc cgcaccgcgg tggtccacgg
     2221 ggtcgagaag ctgaccggcg ccgaagtgat ggcgaccgac ctgcgcgcct cgatgagcct
     2281 ggtgatcgcc ggcct
//
LOCUS       DASUDV010000426         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2156895,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000426 DASUDV010000000
VERSION     DASUDV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156895"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFV30_09275"
     CDS             complement(<1..366)
                     /locus_tag="VFV30_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX4848323.1"
                     /translation="MSNERLSGLDALRGIAALCVVGLHANAVYSQTLPTWFTKGYLGV
                     DFFLMLSGYLMARITEPRLARGLTPANFIKARYLRFWKVVALSSLVGIPYLWWRTQHD
                     PAWFAGAFAANMVFLPFPAD"
     gene            complement(363..1346)
                     /locus_tag="VFV30_09280"
     CDS             complement(363..1346)
                     /locus_tag="VFV30_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX4848324.1"
                     /translation="MIWLRAILVALAMALLARPALADELRPGYIELTQQDASHWRMIW
                     KAPVLGGLATRASPRVPEFCTQSKPDARLQGTSVVAQFTLTCSRDLAGSQVGLSGMEA
                     SFTDALVRIAPLGRPVQAARLTQDEPTITVATTPDRWEVARNYFVIGVEHILFGWDHL
                     LFVIALVLLLGRLWNVVKAATAFTLAHTITLAGTTMGLFGLPQAPVEALIALSIVFLA
                     VEIVKKREDAPRLSERVPWIVAFVFGLIHGFGFAGALREIGLPEGEVPMALLTFNIGV
                     EAGQLVIIAVVLGLIATTRRFAPAALRPATLAASYAVGTIASFWFIERMVG"
     gene            complement(1343..>2044)
                     /locus_tag="VFV30_09285"
     CDS             complement(1343..>2044)
                     /locus_tag="VFV30_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4848325.1"
                     /translation="DEVVVSSLVQRFYDSFRRPPSQDEIDGMIRDYVRDQVYYREALS
                     LGLDQNDEVVIKRMRRKMESLAVADAEARNPSDAQLQALLDKNPATYVIDPRTSFEQI
                     YLGADSPENRLLAEARLATLRKGGQVEAVPAPIPAQLQNAPAAEISATFGDEFTLALR
                     DLPQGQWSGPVPSGVGLHLVRVTGRSAPEKPSLASVRQRLENDWRATEIGKAQEKAYA
                     DMLEGYDVVIEKPKG"
BASE COUNT          378 a          662 c          678 g          326 t
ORIGIN      
        1 gtcggccggg aagggcagga acaccatgtt ggcggcgaag gcgccggcaa accaggccgg
       61 atcgtgctgg gtgcgccacc acagataggg aatgccaacc agcgaagaca gcgccacaac
      121 cttccagaag cgcaggtagc gtgccttgat gaagttcgcc ggggtcagcc cgcgggccag
      181 ccgcggctcg gttatccggg ccatcagata gccggacagc atcaggaaga aatcgacccc
      241 gaggtagcct ttggtgaacc aggtcggcag ggtctgcgag taaaccgcat tggcgtgcag
      301 accgacgacg cacagggcgg cgataccgcg aagcgcatcg agcccgctca gccgctcgtt
      361 gctcacccca ccatccgctc gatgaaccag aagctggcaa tcgtgccgac cgcgtagctg
      421 gccgccagcg tcgcgggccg cagcgccgcc ggggcaaagc gccgggttgt agcgatcagg
      481 cccagcacga ccgcgataat caccaactgt ccggcttcaa ccccgatgtt gaaggtcagc
      541 agcgccatcg gcacctcgcc ttcgggcagg ccgatttcgc gcagcgcccc ggcgaagccg
      601 aaaccgtgga tcaggccgaa gacgaaggca acgatccacg gaacccgctc ggacaggcgc
      661 ggggcatctt cgcgtttctt gacgatttcc accgcgagaa acacgatcga gagcgcaatc
      721 agcgcctcga ccggggcctg gggcaggccg aacaggccca tggtcgtacc cgccagggtg
      781 atcgtgtgcg ccagggtaaa ggcggttgcg gccttgacca cgttccacag ccggcccagc
      841 aacagcacca gcgcgatgac gaacagcagg tgatcccagc cgaacaggat gtgttcgacg
      901 ccgatcacga aatagttgcg cgccacttcc cagcgatcgg gcgtggtggc gacggtgatc
      961 gtcggttcat cctgggtcag ccgcgccgcc tggacgggcc ttcccagcgg tgcgatccgg
     1021 accagcgcat cggtgaagct cgcctccatc ccgctcagcc caacctggct tccggccaga
     1081 tcgcggctgc aggtgagggt gaattgggcg acgaccgagg tgccctgaag ccgggcatcg
     1141 ggtttggact gggtgcagaa ttccggaacc ctggggctcg cccgggtggc aaggccgccc
     1201 agcaccgggg ccttccagat catccgccag tggctggcat cctgctgggt cagttcgatg
     1261 tagccggggc gcaattcatc ggccagtgcc gggcgggcca gcagcgccat ggcaagggcc
     1321 acaaggatcg cgcgcaacca gatcatccct tgggcttttc gatcaccacg tcatagcctt
     1381 cgagcatgtc ggcataagcc ttctcctggg ccttgccgat ttccgtcgcc cgccagtcgt
     1441 tctcaagccg ctggcggacg ctggccaggc tgggcttttc cggagcagaa cgccccgtca
     1501 cccgcaccag gtgcaggccg acgcccgaag ggaccggacc ggaccactga ccttgcggca
     1561 gatcgcgcag cgcgagggtg aattcatcgc cgaaggttgc gctgatttcg gccgccgggg
     1621 cgttctgcag ctgcgcgggg atcggtgcgg gcacggcttc gacctgccca cccttgcgca
     1681 gggttgccag ccgggcctcg gcaagcaggc ggttttcggg gctatctgcc ccaagataga
     1741 tctgttcaaa gctggtgcgc ggatcgatca cataggtggc cgggttcttg tcgagcaggg
     1801 cttggagctg tgcgtcggat gggttgcgcg cctcggcatc ggccacggcc agggactcca
     1861 tcttgcgccg catgcgcttg atcacgacct cgtcgttctg gtcaagccca agcgaaagtg
     1921 cttcgcggta atagacctga tcgcgcacat aatcgcggat catcccgtcg atctcgtcct
     1981 ggctcggggg acggcggaag ctgtcataga agcgctggac gaggcttgag acgacaacct
     2041 cgtc
//
LOCUS       DASUDV010000427         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_942058,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000427 DASUDV010000000
VERSION     DASUDV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942058"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VFV30_09290"
     CDS             complement(<1..128)
                     /locus_tag="VFV30_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEX4848326.1"
                     /translation="MIEARIASGVGALPAAEWDALAGGNPFVSHAFLSALEDSGSVG"
     gene            complement(125..904)
                     /locus_tag="VFV30_09295"
     CDS             complement(125..904)
                     /locus_tag="VFV30_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX4848327.1"
                     /translation="MPSLLFALLDRWRAPPPKPEKVSWLGTVAYAHRGLHLPGVPENS
                     LSSFRGAIERGMGIECDVQRSQDGTAMVFHDSELDRLTAETGPIGERGSDELARIELS
                     GSKDRIPTLRRMLDEVAGRVPILIEIKSSRGSFRHAGALCQAVRRTLEGYAGPHAVMS
                     FDPRVVRWFADRSPLTVRGLVVTEEDDKALPGMIRRRLFLWRARPDFLAYDIRDLPSR
                     FAAGQRRRGLPLLTWTVRSAEHRERAAEHADAPIAEGAGVT"
     gene            complement(876..1337)
                     /locus_tag="VFV30_09300"
     CDS             complement(876..1337)
                     /locus_tag="VFV30_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4848328.1"
                     /translation="MSIEARLAELGITLPEPAAPVAAYVPVVIAGGLAHVSGQVSFVD
                     GVLVKGRLGEDVTLDQGIAAAQGCGLMILAQLKAALGSLDRVERVVKLGAFINSTAAF
                     TDQPKVANGASELMVAVFGEAGKHARSAVGVPSLPLGVAVEVDAIVAVRPA"
     gene            complement(1347..1961)
                     /locus_tag="VFV30_09305"
     CDS             complement(1347..1961)
                     /locus_tag="VFV30_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4848329.1"
                     /translation="MARPLIISDCDEVLLHMIVPFGEWLEETQPVSFKLIGNDFSKAI
                     RDKVSGEPVEPMEIWRLLNLFFDGEMHRQSPIAGAVGAINTLKEHADVVILTNLADKH
                     NAARARQLSDHGIEARVFTNQGPKGPALQAILDEYQPSKAVFIDDLAQHHGSAAEMVP
                     HIDRLHLCGEPTLAPHITCGFEAGHAHARIDTWEHALPWLLSRL"
     gene            complement(1966..2235)
                     /locus_tag="VFV30_09310"
     CDS             complement(1966..2235)
                     /locus_tag="VFV30_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HEX4848330.1"
                     /translation="MLREPKSPAPDPAALALGALGWVLGDEDRAGRLLALTGLTPEAL
                     RGGLGDPAVLGAVLDFLGQYEPDLVAAAAHLGVEPAELMAARERL"
     gene            2272..2646
                     /locus_tag="VFV30_09315"
     CDS             2272..2646
                     /locus_tag="VFV30_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4848331.1"
                     /translation="MAKRILVVEDNDLNRKLFCDVLRSQGYAVEPCADGMLALDAARQ
                     FVPNLIIMDIQLPNVSGLDLIEAAKKDATLRAIPVLAVTAYAGKGDEERIRDAGAEGY
                     LAKPVSIGPFMAAVKGLVEKAA"
     gene            2643..3350
                     /locus_tag="VFV30_09320"
     CDS             2643..3350
                     /locus_tag="VFV30_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848332.1"
                     /translation="MTFPRFAMACLASLFLPACGKSQPAPADAPPSVTSEEVRLSDLG
                     QGGPKLTKGKVYRVVGRVRNIQADPDGSPSITFTGLNLKFAKGRDMATVKQMKPGDFI
                     EADCTWVGVTTGIFPEMTGCTNPAFPQTLSAQDYEAAYAENVFSADDALKDRPLVIFG
                     PVRRVAKLTSGEDFVAIEAQPGFSDVTAIVAPSNRSLIRGYAKPGELTVMYCRGGYRY
                     NQMGSIGVNGCRFLQNY"
BASE COUNT          579 a         1224 c         1156 g          551 t
ORIGIN      
        1 ccgaccgagc cggaatcctc cagcgcggag aggaaggcat ggctgacaaa gggattgccc
       61 ccggccagcg cgtcccactc tgctgcggga agcgcgccga cgcccgatgc gatccgcgct
      121 tcgatcacgt gaccccggcg ccctccgcaa tcggcgcgtc ggcgtgctcg gccgcgcgct
      181 cgcggtgttc ggccgagcgg accgtccagg tcagcagcgg caggccgcgg cggcgctgcc
      241 ccgcggcgaa ccggctgggc aggtcgcgga tgtcataggc caggaaatcg ggccgcgccc
      301 gccacaggaa cagccgccgc cggatcatcc cgggcagggc cttgtcgtcc tcttcggtca
      361 ccaccaggcc gcgcacggtc agcggcgaac ggtcggcaaa ccagcgcacc acccgcggat
      421 caaagctcat caccgcgtgg ggcccggcat agccctccag cgtgcgccgc accgcctggc
      481 acagcgcgcc ggcgtggcga aagctgcccc ggctggactt gatctcgatc aggatcggca
      541 cccgcccggc cacttcgtcc agcatccgcc gcagggtggg gatccggtcc ttgctgcccg
      601 acagttcgat ccgggccagc tcgtcgctgc cgcgttcgcc aatcgggccc gtttcggcgg
      661 tcagccggtc cagctcggaa tcgtggaaga ccatggccgt gccatcctgc gagcgctgga
      721 catcgcattc gatgcccatc ccgcgctcga tcgcgccgcg aaacgaggac agcgaatttt
      781 ccggcacgcc gggcaggtgg agcccgcggt gggcataggc caccgtgccc agccagctga
      841 ccttttcggg cttgggcggc ggcgcgcgcc agcgatcaag cagggcgaac agcaacgatg
      901 gcatccactt ccacggcaac ccccagcggc aggctgggca cgccgaccgc gctgcgggcg
      961 tgcttgccgg cctcgccgaa cacggcgacc atcagttccg aggcgccgtt ggcaaccttg
     1021 ggctgatcgg tgaaggcggc ggtcgaattg atgaaggcgc ccagcttgac cacgcgttcc
     1081 acccggtcga gcgagccgag cgcggccttg agctgggcca ggatcatcag cccgcagccc
     1141 tgcgccgccg caatcccctg atccagcgtc acgtcctcgc ccagccggcc cttgaccagc
     1201 acgccgtcga cgaaagagac ctggcccgaa acatgggcca gcccgccggc aatcacgacg
     1261 gggacatagg ccgccaccgg tgcggcgggt tcgggcaggg ttatgccaag ttcggccagg
     1321 cgtgcttcaa tgctcatgga accatcctac agtcgcgaga gcagccaggg caaggcgtgc
     1381 tcccaggtgt cgatccgggc gtgggcgtgg ccggcctcga acccgcaggt gatgtgcggg
     1441 gccagcgtcg gttcaccgca taggtgaagc cgatcgatgt gaggaaccat ttcggctgcc
     1501 gagccgtggt gctgggccag gtcgtcgatg aacaccgcct tcgacggctg gtactcgtca
     1561 aggatcgcct gcagcgccgg gcccttgggg ccctggttgg tgaagacccg ggcctcgatc
     1621 ccgtgatcgg acagctggcg agcccgggcg gcgttgtgct tgtcagccag gttggtcagg
     1681 atcacgacat cggcgtgttc cttcagcgtg ttgatcgcgc ccaccgcgcc ggcaatcggg
     1741 ctctggcggt gcatctcgcc atcgaagaac aggttcagca gccgccagat ctccatcggc
     1801 tccaccggct cgccgctcac cttgtcgcgg atcgccttgg agaaatcgtt cccgatcagc
     1861 ttgaacgaga ccggctgggt ttcctccagc cattcgccga acgggacgat catgtgcagc
     1921 agcacttcgt cgcaatcgct gatgatcagc ggccttgcca taatcctaca acctctctcg
     1981 cgcggccatc aattcggccg gttcaacccc caggtgcgcc gccgcggcga ccagatcggg
     2041 ctcatactgc cccaggaaat ccagcaccgc gcccagcacc gccggatcgc ccagcccgcc
     2101 gcgcagcgcc tcgggcgtca ggcccgtcag cgccagcagc cggccggccc ggtcctcgtc
     2161 gcccagcacc cagccgagcg cacccagggc cagcgcggcg gggtccggag cgggggattt
     2221 gggttcgcgc agcactagtc acgccttcgg ccagggggta gggcttgtcc catggcaaag
     2281 cgtatcctgg ttgtcgagga caacgacctc aaccgcaagc tgttctgcga tgttctgcgc
     2341 agccagggct atgcggtgga gccctgcgcc gacggaatgc tggcgctgga cgccgcgcgg
     2401 cagttcgtgc ccaacctgat catcatggac atccagctgc ccaatgtttc cgggctggac
     2461 ctgatcgaag cggccaagaa ggacgccacc ttgcgcgcca tcccggtcct tgccgtcacg
     2521 gcctatgccg gcaagggcga cgaggaacgg atccgcgacg ccggcgccga gggttacctc
     2581 gccaagccgg tctcgatcgg tccgttcatg gccgcggtga aggggttggt ggagaaggcc
     2641 gcatgacatt tccccggttt gcgatggcct gcctggcctc cctgtttctg ccggcctgcg
     2701 gcaagtctca gccggcgccc gccgatgccc cgccttcggt taccagtgag gaagtgcggc
     2761 tgtccgacct tggccagggc ggcccgaagc tgaccaaggg caaggtctat cgggtggtag
     2821 ggcgggtccg caacattcag gccgatccgg atggcagccc gagcatcact ttcaccgggt
     2881 tgaacctgaa gttcgccaaa gggcgcgaca tggcgacggt gaagcagatg aagcccggcg
     2941 atttcatcga agcggattgc acctgggttg gcgtgacgac ggggatcttc ccggaaatga
     3001 ccggctgcac caacccggcc ttcccgcaga cgctctcggc ccaggactat gaggccgcct
     3061 atgccgaaaa cgtcttcagc gcggacgatg cgctgaagga tcgcccgctg gtgatcttcg
     3121 gcccggtccg gcgtgtggcc aagctgacca gcggcgagga tttcgtggcg atcgaggccc
     3181 agcccggatt cagcgacgtg accgcgattg tcgcgccgtc caaccggtcg ctgatccgcg
     3241 gctatgccaa gccgggggag ctgacggtga tgtattgccg gggcggctac cgttacaacc
     3301 agatgggttc gatcggcgtg aacggctgcc ggttcctgca gaattactag cccgcggccc
     3361 atacggcgcg ccccgctggc ccgcctcaat gcatcctcgc ttgacttttc gcaccccgcg
     3421 ccgcttttcc gccccaatca tcggccaagg ggccagcaca ccataccgga gcatttgaca
     3481 ccatggaccg cgccgccact gccgccaaga
//
LOCUS       DASUDV010000428         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2024650,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000428 DASUDV010000000
VERSION     DASUDV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024650"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VFV30_09325"
     CDS             complement(<1..1224)
                     /locus_tag="VFV30_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4848333.1"
                     /translation="MIAKTIPSRIKTLGLPLGLAGAAAIALAAGAGFSGSVVAKPSPD
                     KAAAAAQQALKGGKIEKAIELAEAVVAANPNEPSYRALLGQAYMKAGRFESAATTFND
                     AMKLGDTSAKTALALALANSAQGRNRDAVAILDDWRDAIPAADLGLALALSGETSRGV
                     AILADALRAGEDTPKIRQNLAYAYALDGRWREARVMAEQDVPADQLDARISDWAARAK
                     PEDHQVRIAGLLNVPVRADPGQPRALALGDNAAREQLAVETAATKAAPQFAANGELAP
                     AGEAPASLAQYRPVDAPAEAAPAYDNPYGAKGASTPIVQSLPQNYAAAEPAQQPVAKA
                     KARPVARKSVAFASHKHGLRPRQGAPKAKYGFGSSHNVQLGSFLSPQGARRAWGIYAA
                     KNPELKGYRMAITQAK"
     gene            complement(1251..1694)
                     /locus_tag="VFV30_09330"
     CDS             complement(1251..1694)
                     /locus_tag="VFV30_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848334.1"
                     /translation="MSDPKPFASLSPTLLARKGGAKPAMRSQLSALPVAEPHEDLGWN
                     DMGHDHMAEIVRLTPEGAPIPEVLRQQQELSNRIARERRSALAAGRRAAFTLRVDSDR
                     HLKLRLACTLHNRSAQQLVTEALDQWLDALPDVAELAAQVTKKRK"
     gene            complement(1709..2428)
                     /locus_tag="VFV30_09335"
     CDS             complement(1709..2428)
                     /locus_tag="VFV30_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4848335.1"
                     /translation="MRVLALASQKGGSGKTTLSGHLAVQAQRSGAGPVVLIDIDPQGS
                     LADWWNEREAEFPAFAQTTVARLASDLAVLRQQGFKLAVIDTPPAITMAIQSVIAVAE
                     LIVVPTRPSPHDLRAVGATVDLCERAGKPLIFVVNAATPKAKITSEAAVALSQHGTVA
                     PITLHHRTDFAASMIDGRTVMEVDPNGRSAAEVAALWNYISDRLEKNFRRTVFAAPTS
                     VSSIQGAARPAGGFGRRVAGS"
BASE COUNT          408 a          795 c          842 g          423 t
ORIGIN      
        1 cttggcctgg gtgatggcca tgcgataacc cttcagttcc gggttcttcg cagcatagat
       61 gccccaggcg cggcgcgcgc cctgcggcga gaggaagctg cccagctgga cattgtgcga
      121 cgaaccgaag ccgtacttgg ccttgggcgc accctggcgc ggccgcaggc catgcttgtg
      181 cgaggcgaac gccaccgact tgcgcgcaac cgggcgagcc ttggccttgg ccaccggctg
      241 ctgggccggc tcggccgcgg catagttctg cggcagcgac tggacgatcg gagtcgatgc
      301 gcccttggcg ccataagggt tgtcataggc cggtgcggcc tcggcggggg catcgaccgg
      361 gcggtactgg gccagcgagg caggtgcttc tccagccgga gccaattcgc cattcgcggc
      421 aaactgcggc gcggcctttg ttgcagcggt ttcgacggca agctgttcgc gggccgcatt
      481 gtcgcccagc gccagcgcgc gcggctgtcc cggatcggca cgtaccggca cgttcagcag
      541 gccggcaatg cggacctgat gatcttcagg cttggcacgg gctgcccaat cgctgatccg
      601 cgcatcgagc tgatcagccg gaacatcctg ttcggccatg acccgggcct cgcgccagcg
      661 cccatcgagc gcataagcat aagccaggtt ctggcggatc ttcggggtgt cttcgccggc
      721 acgcagcgca tcggcaagga tcgccacacc gcggctggtt tcgcccgaca gggcaagcgc
      781 caggccgaga tcggcagccg ggatcgcgtc gcgccagtca tcaaggatcg caacggcatc
      841 gcggttgcgg ccctgggccg aattggcaag cgccagggca agcgccgtct tggccgaggt
      901 atcgcccagc ttcattgcat cgttgaaggt ggtggcagcg gattcaaacc ggcccgcctt
      961 catgtaagcc tgtcccagca gcgcacgata ggacggctcg ttggggttcg cggcaaccac
     1021 ggcttcggcc agttcgatcg ccttttcaat cttgccgcct ttcagcgcct gctgggcagc
     1081 ggccgctgcc ttgtcggggc tgggcttggc gacaacgctg cccgagaaac cggcaccagc
     1141 ggcaagggcg atggcagcag cgccagcaag gcccaacggc agacccaggg tcttgatccg
     1201 gctcggaatg gtcttggcga tcatcggctt cttccttcca ggtagatact ttacttgcgc
     1261 ttcttggtga cctgagcggc cagttcggcc acgtcgggca aggcatcgag ccattggtcg
     1321 agcgcttcgg tgacgagttg ctgggcgctg cggttatgca gggtgcaggc cagccgcaat
     1381 ttgagatggc ggtccgaatc gacccgcagc gtaaaggccg cacggcgtcc cgcggctagg
     1441 gccgagcggc gttcgcgggc aatccggttg ctgagttcct gctgctggcg caggacttcg
     1501 ggaatcggcg ccccttccgg ggtcagccgg acgatttcgg ccatgtgatc gtggcccatg
     1561 tcgttccagc caaggtcttc gtgcggttcg gccaccggca gggccgacag ctggctgcgc
     1621 atcgccggct tggcgccgcc cttgcgtgcg agcaaggtcg ggctcagcga ggcaaagggc
     1681 ttgggatcgc tcaccgcgcc gggcccgttc aggaaccggc gacgcggcgg ccaaagccgc
     1741 cggcggggcg agctgcgccc tgaatcgacg agacgctggt cggcgcggca aagacggtac
     1801 ggcggaagtt cttttccagg cgatcggaaa tgtagttcca cagcgcggcc acttcggcgg
     1861 cactgcggcc gttaggatcg acttccatca ccgtgcggcc atcgatcatc gaggcggcaa
     1921 aatcggtccg gtggtgcagc gtgatcgggg cgacggtgcc atgctgcgag agggcaaccg
     1981 cggcttccga ggtgatcttg gccttgggcg tcgcagcatt gaccacgaag atcagcggct
     2041 tgccagcgcg ttcgcacaga tcgaccgtgg cccccacggc gcgcaggtcg tgcgggctgg
     2101 gacgcgtagg cacgacgatg agttcggcca ccgcaatcac cgactggatc gccatggtga
     2161 tcgccggcgg ggtatcgata accgcaagct tgaagccctg ctgacgcagg acggcaagat
     2221 cgcttgccag acgcgcaacc gtggtctggg cgaaggccgg gaactccgct tcgcgttcat
     2281 tccaccagtc cgccagcgac ccctgcgggt cgatatcgat cagcacgacc ggccccgcgc
     2341 ctgaacgctg ggcctgcacg gcgagatgcc cggacaaggt ggtcttgccc gatccgccct
     2401 tctgcgatgc caaagccaat acgcgcaaag ccattccccc taagaaaatt tggcaaatcc
     2461 aattcgac
//
LOCUS       DASUDV010000429         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_853841,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000429 DASUDV010000000
VERSION     DASUDV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853841"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFV30_09340"
     CDS             <1..972
                     /locus_tag="VFV30_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX4848336.1"
                     /translation="FLLILPEAGLTLSFLMAKSAAAIAAIIPLLRSYGLPRGWRPHPG
                     RTGKLAMHLMPLAGADMVEWGTRKLDIAILGLFTSPAAVGIYYAAQQVASLPQKLKTS
                     FEPILGPVITRNLKEGNHAAIAQQVCQVGFWIIAAQAGVALALGIPGEGVMGLVGPAF
                     VGGTGALLFLLAAEVAAATAVVSEAALIYVARLRNLWVSLGTIALQATLTVAGIELVD
                     HLGLSDNYRAAAAAAALMLSLGFASLVKSRMLAVILGQSINNWRWALIWAAAPAIVVG
                     WVFTRFLPEWAELLFGIPAILLAYGWVIWHKGFGPEDRVLFRKNVGA"
     gene            977..1714
                     /locus_tag="VFV30_09345"
     CDS             977..1714
                     /locus_tag="VFV30_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848337.1"
                     /translation="METIEANWLVFAVVALAALLVAWWLFARASKAPPRAHKPDVLDE
                     GVGPAQRNQALIDAPPAAQIVTPPPLSGTMAGIGEAIAVAAQDQVEEVEAKAQEPAPP
                     PPPPPPPPPPPPPPPPAPGPVEVPEHEPEPQPEPEPEPEPEPEPQPEAAPEPEPAPEP
                     AGAEPSGADDLSRIKGLGPKLQALLPTLGITTFAQIAAWTEEDLARIDPQLGPFAGRP
                     ARDNWIEQAKYLAAGDVAGFEDKFGKV"
     gene            complement(1725..>2034)
                     /locus_tag="VFV30_09350"
     CDS             complement(1725..>2034)
                     /locus_tag="VFV30_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX4848338.1"
                     /translation="VADTASRDGVLVEIEDDERNNLVGHNLRAYIEELEGRMRAAAAD
                     LELEEAGRLRDEIRRLEATELGLPEGEHRAPIVGRSNEGKPGTRKGRFGRQGRTKWGR
                     "
BASE COUNT          306 a          694 c          682 g          352 t
ORIGIN      
        1 ttcctgctga tcctgcccga agccgggctg acgctttcgt tcctcatggc caagagcgcg
       61 gcggcaattg cggcaatcat cccgctgctg cggtcttatg gcctgccgcg cgggtggcgc
      121 ccgcatcctg gccggaccgg taaactcgcg atgcacctga tgccactggc gggggccgac
      181 atggtcgaat ggggcacacg caagctcgat atcgcgatcc tgggcctgtt cacctcgccg
      241 gccgcggtcg ggatctacta cgcggcgcag caggtcgcct cgttgccgca gaagctcaag
      301 accagtttcg agccgatcct gggtccggtg atcacccgca acctcaagga aggcaaccac
      361 gccgcgatcg cccagcaggt gtgccaggtg gggttctgga tcattgccgc ccaggccggg
      421 gtggcgctgg cgctgggcat tccgggtgaa ggggtgatgg ggctggtcgg cccggccttc
      481 gtcggcggca ccggggccct cctgttcctg ctggcggctg aagtcgccgc cgcaaccgct
      541 gtagtgagcg aggcggcgct gatctatgtc gccaggctgc gcaacctttg ggtctcgctc
      601 ggcacgatcg ccctgcaggc gaccctgacc gtggccggaa tcgaactggt cgatcatctg
      661 gggcttagcg acaactaccg cgcggccgca gcggcggcgg cgctgatgct gtcgctgggc
      721 ttcgcctcgc tggtcaagtc gcggatgctc gccgttatcc tgggccaatc gatcaacaac
      781 tggcgctggg cactgatctg ggcggcggca ccggcgattg tggtaggctg ggtattcacc
      841 cggttcctgc ccgaatgggc cgaactgctg ttcggcattc cggcgatcct cttggcctat
      901 ggctgggtaa tctggcacaa gggttttggc ccggaagatc gggtgctgtt ccgcaagaac
      961 gtgggagctt gaacgcatgg aaacgatcga ggccaattgg ctggttttcg ccgtggtggc
     1021 cctggccgcg ctgctggttg cctggtggct gttcgctcgg gccagcaagg cgccgccacg
     1081 cgcgcacaag ccagatgttc tagacgaggg cgttggcccg gcgcagcgca accaggcgct
     1141 gatcgatgcc ccgcccgccg cgcagatcgt cacccccccg ccgctgtcgg gaaccatggc
     1201 cgggatcggt gaggcgattg ccgttgccgc acaggatcag gttgaagagg tagaggccaa
     1261 ggcacaggaa cctgcgccgc cgccgccgcc gccgccgccg ccgccgccgc cgccgccgcc
     1321 gccgcctccc gctcctgggc cggtggaagt gccggaacat gagcccgagc ctcaacctga
     1381 gcccgagccc gagccagagc cagagccaga gcctcagccc gaagcagcgc cggaacccga
     1441 gccagccccg gagccggccg gagccgaacc atcaggcgcg gacgatctct cgcggatcaa
     1501 gggccttggt cccaagctgc aggcgctgct gccaacgctg gggatcacca ccttcgcgca
     1561 gatcgctgcc tggaccgagg aagaccttgc ccggatcgat ccgcaactgg gccccttcgc
     1621 gggccgcccg gcgcgcgaca actggatcga acaggccaaa tacctcgccg ccggcgatgt
     1681 ggccgggttc gaagacaagt tcgggaaggt ttaggcctga acgcctagcg gccccatttc
     1741 gtccggcctt gccggccaaa ccggcccttg cgggtccccg gcttgccctc gttactgcgc
     1801 cccacaatcg gcgcgcggtg ttcgccttcg ggcaggccaa gttcggtggc ttccagcctt
     1861 ctgatctcgt cgcgcagcct tccggcttcc tctaattcga gatcggcggc ggcggcgcgc
     1921 atccgccctt cgagttcctc gatataggcg cgcaggttgt ggccgacgag attgttgcgt
     1981 tcgtcgtcct cgatctcgac cagcacgccg tcccttgatg ccgtgtcggc aaca
//
LOCUS       DASUDV010000430         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1516690,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000430 DASUDV010000000
VERSION     DASUDV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516690"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFV30_09355"
     CDS             complement(<1..416)
                     /locus_tag="VFV30_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4848339.1"
                     /translation="MDFRFTEDQLTLTESVRAYLAGTHGPEVLRRLDEQGNRDPAIWQ
                     GLVEMGLTGLLVPEEHGGLGMGLVEAALIAAECGRACLAEPLVDTAFVAVPWLVQQDD
                     TEGLQAIAEGTRTVPLPHVLNPWVADGAGQPLQSVDP"
     gene            complement(416..1579)
                     /locus_tag="VFV30_09360"
     CDS             complement(416..1579)
                     /locus_tag="VFV30_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4848340.1"
                     /translation="MQLEYTPEQQAFRAEVRAWMEAHVPKEPLVTLECREGYDQHVAW
                     ERTLASGNWGMVTWPEQYGGRGLDLIQWLIFEEEYFRAGGPNRANQNGIFLLGPTIME
                     FGTSEQKQRFLPPMARGEVIWAQAWSEPGAGSDMAAIQSKAVRDGDHYVITGQKTWSS
                     RAAFADWGFGIFRTDPDSKRHKGLTFILFDLNSPGITRRPIRQLHGDTGFAELFFDEV
                     RVPVENCLAGEGEGWNVAMATAGFERGLMLRSPGRFQATAKRLVELYRAHEAEASPAA
                     REAVVQAWGAAQAYAYNTYAVASKIMAGGKIGAEASLNKIFWSELDRSMHRTAMQILG
                     AHAELKRFGNGQINQWLEGYIFSLSGPIYAGSNEVQRNIIAERLLGLPRVGAG"
     gene            complement(1579..>2145)
                     /locus_tag="VFV30_09365"
     CDS             complement(1579..>2145)
                     /locus_tag="VFV30_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4848341.1"
                     /translation="DIKEMQAHPERITVLNRGNYLTFKAIRDAEVPVVVAVHKFVIGG
                     GIGICGASDTIIAADDAFFSLPEVDRGAMGGASHLSRMLPLHKVRAAFFTGGNIPVAE
                     AYRLGAVEKVVPRADLVAEARAFCAVVASKSRKALVIAKEALNGLEPRDVDRGYRWEQ
                     GFTLEMYMHEDSQKSRDAFVETGKAAKF"
BASE COUNT          326 a          736 c          710 g          373 t
ORIGIN      
        1 ggatcgacgc tttgcagcgg ctggcccgcg ccgtcggcca cccaggggtt gagcacgtgg
       61 ggcaggggca ccgtgcgggt gccctcggcg atggcctgca ggccctcggt gtcatcttgc
      121 tgaaccagcc agggcaccgc gacgaaggcg gtatcgacca gcggctcggc caggcaggcc
      181 cgcccgcatt ccgccgcgat cagcgccgcc tcgaccaggc ccatgcccag cccgccgtgc
      241 tcttcaggca cgagcaggcc ggtcagcccc atctcgacca ggccctgcca gatcgcggga
      301 tcgcggttgc cctgctcgtc cagccggcgc agcacttcgg ggccgtgggt gcccgccaga
      361 taggcgcgga cagactcggt cagggtcagc tgatcttcgg tgaaacggaa gtccatcagc
      421 ctgctcccac ccgcggaagg cccagcagac gctcggcgat gatgttgcgc tgaacctcgt
      481 tcgatccggc gtagatcggg ccggagagcg agaagatgta gccttccagc cactggttga
      541 tctgcccgtt gccgaaccgc ttcagctcgg catgggcgcc caggatctgc atcgcggtgc
      601 ggtgcatcga ccggtccagt tccgaccaga agatcttgtt gaggctggct tccgcgccga
      661 tcttgccgcc ggccatgatt ttcgaggcga cggcataggt gttgtaggca taggcctgcg
      721 ccgcgcccca ggcctgtacc accgcttcgc gcgcggcggg ggaggcttcg gcctcgtgcg
      781 cgcggtagag ctcgaccagc cgcttggcgg tggcctggaa gcggcccggc gagcgcagca
      841 tcagcccgcg ttcgaacccg gccgtggcca tcgccacgtt ccagccttcg ccttcgccgg
      901 ccaggcaatt ctcgaccggt acccgcacct cgtcgaagaa cagctcggca aagccggtgt
      961 cgccgtgaag ctggcggatc gggcggcggg tgatgccggg gctgttcaga tcgaacagga
     1021 tgaaggtcag ccccttgtgg cgcttgctgt ccggatcggt acggaaaatg ccaaagcccc
     1081 aatcggcaaa ggcggcgcgg ctggaccagg tcttttgccc ggtgatgacg taatggtcgc
     1141 cgtcgcgcac cgccttggac tggatcgccg ccatgtcgct gcccgcgccg ggttccgacc
     1201 aggcctgggc ccagatcact tcgccgcgcg ccatcggggg caggaagcgc tgcttctgct
     1261 ccgaggtgcc gaattccatg atcgtcgggc caagcaggaa gatgccgttc tggttggccc
     1321 ggttgggacc gccggcgcgg aagtattcct cctcgaagat cagccactgg atcaggtcca
     1381 gcccgcgccc gccgtactgc tcgggccagg tgaccatgcc ccagttgccg ctggcgagcg
     1441 tgcgctccca cgccacgtgc tggtcatagc cttcgcggca ttccagcgtg acgagcggct
     1501 ccttcggcac gtgcgcttcc atccaggcgc gcacttccgc ccggaacgcc tgttgttcgg
     1561 gggtgtattc cagctgcatc agaacttcgc cgccttgccg gtctcgacga aggcatcgcg
     1621 gctcttctgg ctatcctcgt gcatgtacat ttcgagcgtg aagccctgtt cccagcggta
     1681 gccgcgatcg acatcgcgcg gctccagccc gttcagcgct tccttggcga tcaccagcgc
     1741 cttgcggctc ttgctggcga cgacggcgca gaaggcgcgg gcctcggcca ccaggtcggc
     1801 gcgcggaacg accttttcga ccgcgcccag ccggtaggct tcggcgaccg gaatgttgcc
     1861 gccggtgaag aaggcggcgc ggaccttgtg cagcggcagc atgcgcgaga ggtggctggc
     1921 cccgcccatt gcgccgcgat caacctcagg caggctgaag aaggcgtcat cagcggcgat
     1981 gatcgtgtcg ctcgctccgc aaatcccgat cccgccgccg atcacgaact tgtgcacggc
     2041 caccaccacc ggcacttcgg catcgcggat tgccttgaag gtcaggtaat tgccgcggtt
     2101 cagcacggtg atccgttccg ggtgcgcctg catctccttg atatc
//
LOCUS       DASUDV010000431         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_434162,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000431 DASUDV010000000
VERSION     DASUDV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434162"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..693)
                     /locus_tag="VFV30_09370"
     CDS             complement(76..693)
                     /locus_tag="VFV30_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX4848342.1"
                     /translation="MGLSAEQIRDGLDAVAAREPRIGEALAKAGYPEPRIRERGHGTL
                     MRTIVGQQVSVAAAASMWRKFEAHVGPELDPLRVLDSDFDTLRACGLSRQKQGYMRSL
                     CELVVSGGLDFHALPADDEEAIAQLTQIKGIGRWSAEIYLLFAEGRPDIWPAGDLAVQ
                     AGLAKLLGLDERPSEKATRELAEKWRPHRGAAAIFTWHCYNNPAL"
     gene            794..1111
                     /locus_tag="VFV30_09375"
     CDS             794..1111
                     /locus_tag="VFV30_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4848343.1"
                     /translation="MPKLVVVNRAGEEKEIEASAGVSVMEAIRDNGFDELLALCGGCC
                     SCATCHVYVDPAFADKVTPLSEDENDLLDSTDHRTDHSRLSCQLEISDALDGLKVTIA
                     PED"
     gene            1174..2181
                     /locus_tag="VFV30_09380"
     CDS             1174..2181
                     /locus_tag="VFV30_09380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX4848344.1"
                     /translation="MTKPLVQSSSLDLIGNTPLVLLQGPSEAAGCEIWGKCEFANPGA
                     SVKDRAALWIVRDAESRGALKPGGTIVEGTAGNTGIGIALVANALGYKTVIVMPETQS
                     REKTDTLRALGAELVLVPAAPFSNPGHFVHTSRRLAEETEGAVWANQFDNIANRKAHI
                     ESTAPEIWEQMDGRIDGFTCAAGTGGTIAGVGMGLKAFDENVRIALTDPHGAALYNYY
                     AHGELKAEGSSVAEGIGQGRITANLEGAPIDTQFRISDEEGLEWVARLLQEEGLCLGL
                     SSGINVAGAVALGRQLVAEGREDPRVATILCDTGFRYLSSLYNAEWLKVKGLPVFPWL
                     A"
     gene            2203..2538
                     /locus_tag="VFV30_09385"
     CDS             2203..2538
                     /locus_tag="VFV30_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848345.1"
                     /translation="MASQPPRRVVEGLSGPQHFVPPTARELRAQAVQRLQTGLFGIAA
                     VLLVVGLANIINDRARMAEAANKPAAQASASASPTNSDPLADIGVVPTSAPSASPSAS
                     ASPTANAPR"
     gene            2525..3277
                     /locus_tag="VFV30_09390"
     CDS             2525..3277
                     /locus_tag="VFV30_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX4848346.1"
                     /translation="MLRAKPRPLWGVLLILGAVLAAIAWFAQPRRAASAEPVGLFTTL
                     PILWSENPDIGAALSPDAQPHWAREALGTAGPVEPLDTLAGAGPTPPLARIRRLVMAQ
                     PRVLTPAENVALDEWVRGGGQLLLLADPALTEDSLFPIADPRRPQAVVLLSPILARWG
                     LELRIADGQPFGETRPEVMGVPVPVNLPGHFVTEGQGNCRLWADGLAVTCAIGKGRVV
                     AVADAAVLERADPDRARRKALLWLLDAAFAAR"
     gene            3621..4127
                     /locus_tag="VFV30_09395"
     CDS             3621..4127
                     /locus_tag="VFV30_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX4848347.1"
                     /translation="MAEAAKPIMYSGQGFSLVGEKGRFVLPPDFRKAVRDSGQGDRVL
                     CLAKHPRWKCLTAFGLGRVAQFQAELDREERIALERGLDFDRELRANQLYGFAQVPFD
                     DSGRFILPERFFKLGAIADAIYFQGGGLQFTIWNPDELAKMGAGWEDAQEACLDLAAK
                     AVAGKGRK"
     gene            4124..>4810
                     /gene="rsmH"
                     /locus_tag="VFV30_09400"
     CDS             4124..>4810
                     /gene="rsmH"
                     /locus_tag="VFV30_09400"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX4848348.1"
                     /translation="MTPAAPHIPVLLDEVVAALRPAPGTVIVDATFGAGGYTRRLLDA
                     GATVHAFDRDPDAIAAGSGWSETAESPPRLVLHPRRFSEMLAALADAGVARVDGVVMD
                     IGVSSMQLDQAERGFAFSSDGPLDMRMGQEGESAADFLNTAAESTIADVLFEYGEERQ
                     SRRVARAIVAARPLETTGQLAHVVRKALGYRPHSNKGPAPKDPATRSFQAIRIHVNAE
                     LDELRAGLSAA"
BASE COUNT          776 a         1620 c         1644 g          770 t
ORIGIN      
        1 tcgagcggat gatgcgcgat cgcaccggct gtgcggtcct gctcgggcaa gccgaggtcg
       61 attagcgggc cagccctaga gcgccggatt gttgtagcaa tgccaggtga agatcgcggc
      121 ggcgccgcgg tgcgggcgcc atttctccgc caattcgcgg gtggcctttt cgctggggcg
      181 ttcgtccagg cccagcagct tggccaggcc cgcctggacc gccagatcgc ccgccggcca
      241 gatgtcgggg cgcccttccg cgaacagcag atagatctct gccgaccagc gcccgatccc
      301 cttgatctgg gtgagctggg cgatcgcctc ctcgtcgtcg gcgggcaggg cgtggaaatc
      361 gagcccgcct gagacgacca gttcgcacag cgagcgcatg tagccctgct tctggcgcga
      421 gaggccgcag gcgcgcagcg tatcgaaatc ggaatcgagc acccgcagcg gatcaagctc
      481 cgggccgaca tgggcttcga acttgcgcca catcgatgcc gccgccgcca cgctgacctg
      541 ctggccgacg atggtccgca tcagggtgcc gtggccgcgt tcgcggatgc gcggttcggg
      601 atagccggcc ttggccagcg cttcaccaat ccgcggctcg cgtgccgcga ccgcatccag
      661 cccgtccctg atctgctctg cgctgaggcc catcgcggtt ttcgcccttc cgtgcggctt
      721 gccaaacttt aagcgagtgg ttagcagccc ggccaaagcg ccgatagggt gccagtcaga
      781 aggatcgtcg gatatgccca agcttgtcgt ggtcaatcgt gccggtgagg aaaaggaaat
      841 cgaagccagc gccggcgttt cggtgatgga agcgatccgc gacaacggct ttgacgaact
      901 gctggcgctg tgcggcgggt gctgcagctg cgccacctgc cacgtctatg tcgatccggc
      961 ctttgccgac aaggtgaccc cgctgagcga ggacgagaac gacctgctcg attcgaccga
     1021 tcaccgcacc gaccactcgc gcctgtcgtg ccagctggaa atcagcgacg cgctggacgg
     1081 gctgaaggtg acgatcgccc ccgaagactg agtttcagcg ggcaaagcgc aaatccgttg
     1141 cgcttgggcc gctgcgaacc tacctctcgc cgcatgacca agccgctcgt ccagtcttca
     1201 agcctcgatc ttatcggcaa cacgccgctc gtcctgctgc aggggccgag cgaagcggcc
     1261 gggtgcgaga tctggggcaa gtgcgaattc gccaaccccg gcgcctcggt gaaggaccgg
     1321 gccgcgctgt ggatcgtccg cgatgcggaa agccgcggtg cgctgaagcc gggcggcacg
     1381 atcgtcgaag gcacagctgg caacaccggg atcgggatcg cgctggtcgc caacgcgctg
     1441 ggctacaaga cggtcatcgt catgcccgaa acgcagagcc gcgagaaaac ggacaccctg
     1501 cgcgcgctgg gggcggaact ggtgctggtg ccggccgcgc cgttttccaa tcccggccac
     1561 ttcgtccaca cctcgcgccg cctggcggag gaaaccgagg gcgcggtatg ggccaaccag
     1621 tttgacaaca tcgccaaccg caaggcccat atcgaaagca ccgcgccgga aatctgggag
     1681 cagatggacg ggcggatcga cgggttcacc tgcgccgcgg gaaccggcgg gaccattgcc
     1741 ggggtcggca tggggctgaa ggcgtttgac gagaatgtcc gcatcgcgct gaccgatccg
     1801 cacggtgcgg cgctctacaa ctattacgcc cacggcgagc tcaaggccga agggtcttcg
     1861 gttgccgaag ggatcgggca aggccggatc accgccaatc tggagggcgc gccgatcgac
     1921 acccagttcc gcatctcgga cgaggaaggc ctcgaatggg tggcccgtct gctgcaggaa
     1981 gaagggctgt gcctgggcct gtcatccggg atcaacgtgg ccggggccgt tgcgctgggc
     2041 cgccagctgg tggccgaagg gcgcgaggat ccgcgggtcg cgacgatcct gtgcgatacc
     2101 ggcttccgct atctctccag cctctacaac gccgaatggc tgaaggtgaa gggactgccg
     2161 gtcttcccct ggctggcctg atcggctagg ctccgccgcg agatggcaag ccaaccgcca
     2221 cgcagggtcg tagaaggact gtccggtccg cagcatttcg tgccgccgac ggcgcgcgag
     2281 ctgcgcgcgc aggccgtgca gcggctccag accgggctgt tcgggattgc cgccgtgctg
     2341 ctggtcgtgg gcctcgccaa catcatcaac gaccgggccc ggatggccga agcggccaac
     2401 aagccggccg cccaggcttc ggcctcggcc tcgcccacca attccgatcc gctggccgat
     2461 atcggcgtgg tgcccacttc ggcccccagc gcatcgcctt ccgcctcggc ttcgcccacg
     2521 gccaatgctc cgcgctaaac cgcgcccgct gtggggcgtg ctgctgatcc tgggcgccgt
     2581 gctggcggcg atcgcctggt ttgcgcagcc gcgccgggcg gcctcggcag agccggtggg
     2641 cctgttcacc accctgccga tcctgtggtc ggaaaacccc gacatcggcg ccgcgctcag
     2701 ccccgatgcc cagccgcact gggcgcgcga ggccctgggc acggccgggc cggtcgaacc
     2761 gcttgatacc ctggccggag ccggtcccac gccgccgctg gcgcggatcc ggcggctggt
     2821 catggcccaa ccgcgggtgc tgaccccggc cgaaaacgtg gcgctggacg aatgggtgcg
     2881 gggcgggggg cagctgctgc tgctggccga tccggccctg accgaggact cgctgttccc
     2941 gatcgccgat ccgcgccggc cgcaggcggt ggtgctgctg tcccccatcc tggcgcggtg
     3001 gggtctggaa ctgcggatcg ccgatggcca gccttttggc gagacccggc ccgaagtgat
     3061 gggcgtgccg gtgccggtca acctgcccgg ccatttcgtg accgaggggc agggcaattg
     3121 ccggctctgg gccgatgggc tggcggtgac ctgcgcgatc ggcaagggcc gggtggtggc
     3181 cgtggccgat gccgcggtgc tggagcgcgc ggatcccgac cgtgcccgcc gcaaggcgct
     3241 gctctggctg ctcgacgcgg ccttcgccgc gcgttgattc gcgccaaacg gggaaatcgc
     3301 gggaataatc atccggtttg gcggagtcgc ggcgtaagtg cttgattttc gtcttgaaca
     3361 aaccggaaac acggataggt ctggctgata gggtgacgaa tcaaaataag gtaaatcaac
     3421 tatttaccgc gtaatcccgt gaaatcccgc aattttccct ttcatccccg gccggtgaac
     3481 ggtaaggaac cgtttcatcg acaacgaatt ccagagccgt gtcccgcaag gggcagtccg
     3541 gatgcgaggg cgcgcgtccg gaggcggggg aggtcattcc aaggggtccg ggagaaatcc
     3601 cggtttgagg gcgggacgaa gtggcagagg cggccaagcc gatcatgtac agcggccaag
     3661 gcttctcgct ggtgggcgag aagggccgtt tcgtgctgcc gcccgatttc cgcaaggccg
     3721 tgcgcgattc cggacagggt gatcgggtgc tttgcctggc caagcatccc cgctggaaat
     3781 gcctgaccgc cttcgggctg ggccgcgttg cccagttcca ggccgaactg gaccgcgaag
     3841 aacggatcgc gctggagcgc ggcctcgatt tcgaccgcga actgcgcgcc aaccagctct
     3901 acggttttgc ccaggtcccg tttgacgaca gcggccgctt catcctgccc gaacgcttct
     3961 tcaagctcgg cgcgattgcc gatgcgatct acttccaggg cggcggcctg cagttcacca
     4021 tctggaaccc ggacgagctc gccaagatgg gcgccggctg ggaagacgcc caggaagcct
     4081 gcctcgatct ggccgccaag gcggtggccg ggaagggccg caaatgacgc ccgccgcgcc
     4141 ccacatcccg gtcctgctcg atgaagtcgt tgccgcgctc cggcccgccc ccggaacggt
     4201 gattgtcgat gccaccttcg gcgccggcgg ctacacccgc cgcctgcttg atgccggcgc
     4261 cacggtccac gccttcgacc gtgatcccga tgccatcgcc gccggatcgg gttggagcga
     4321 aaccgccgaa agcccgccgc ggctggtgct ccacccccgc cgcttctcgg aaatgctggc
     4381 cgccctggcc gatgccggcg tggcccgggt cgatggggtg gtgatggaca tcggggtgtc
     4441 ctcgatgcag ctcgaccagg ccgagcgcgg ctttgccttc tcctccgatg gcccgctcga
     4501 catgcggatg gggcaagagg gggaaagcgc cgccgatttc ctcaacaccg ccgccgaaag
     4561 caccatcgcc gacgtgctgt tcgaatacgg ggaagagcgc cagtcgcgcc gcgtggcccg
     4621 cgcgatcgtg gcggcgcgtc cgctggaaac caccggccag ctggcccatg tggtgcgcaa
     4681 ggcgctgggc tatcgccccc attcgaacaa gggccccgcg cccaaggatc ccgccacccg
     4741 cagctttcag gcgatccgca tccacgtgaa cgccgaactg gacgaactgc gcgccggcct
     4801 gtccgccgcc
//
LOCUS       DASUDV010000432         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_677308,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000432 DASUDV010000000
VERSION     DASUDV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677308"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VFV30_09405"
     CDS             <1..1378
                     /locus_tag="VFV30_09405"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX4848349.1"
                     /translation="YVRGNPLDFERWQDEDGARGWGYADVLPYFKRAETRAEGGNEYR
                     GGDGPLSTTYGTLKNPLNQAWLDAARQAGYELTEDYNGYRQEGFGRMDMTVRNGTRCS
                     AAKAYLYEARKRPNLHVIQHALATRVLFEGTRAVGVEYERGGTLHQVRASREVLLSGG
                     SINSVQLLKLSGIGPAEELKGVGIEVLADRPGVGANLQDHLEFYFQMACTKPVTLFGK
                     ANLLGKAMVGAEWLFLRRGIGASNQFESCGFIRSRAGIKYPDIQYHFFPMAINYDGSS
                     LATEHGFQAHVGPMRSKSRGTVRLRSKDPREHPKILFNYMSHPDDWAEMRACVRLTRE
                     IFQQDAFAPWRGREIQPGADCVSDEAIDAFIADHVESALHPSCTCKMGDARDPMAVVD
                     PELRVIGVEGLRVIDSSVMPSITTGNLNAPTIMIGEKGADHVLGKGLLPPSNAPYYVA
                     PNWEMSQR"
     gene            1375..>2048
                     /locus_tag="VFV30_09410"
     CDS             1375..>2048
                     /locus_tag="VFV30_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4848350.1"
                     /translation="MSRTITEPARAIPIVHETEVLVVGSGPGGLAAALASARAGVTTT
                     LVERYGCFGGNITQVGVEGFAWYRHPATIDSEGIGREFEDRAKAMGAALPEPQSISHA
                     LDAEGFKYVADCLVEEAGVIPMLHRMFVAPIMEGDTIRGVIVESKAGREAILAQRVID
                     ASGDADIAHRAGAPTHMHPKAEMMSVSVMFSMCGVNKGRFIDAVKADPQTYADWAKDG
                     EWSVTTS"
BASE COUNT          359 a          660 c          714 g          315 t
ORIGIN      
        1 ttacgtgcgc ggcaacccgc tcgatttcga gcgctggcag gacgaggacg gcgcgcgcgg
       61 ttggggctat gccgatgtcc tgccctattt caagcgggcc gaaacccggg ccgagggcgg
      121 caacgaatac cgcggcgggg acggcccgct gtccaccact tacggtacgc tcaagaaccc
      181 gctcaaccag gcctggctcg atgccgcgcg gcaggccgga tacgagctga ccgaggatta
      241 caacggctac cgccaggagg gctttggccg gatggacatg acggtgcgca acggcacccg
      301 ctgctccgcc gccaaggcct atctctatga agcgcgcaag cggcccaatc tccacgtgat
      361 ccagcacgcg ctggccaccc gggtgctgtt cgaaggcacc cgcgcggtgg gggtggagta
      421 cgagcgcggc ggcacgctgc accaggttcg ggcgagccgc gaggttctgc tctcgggcgg
      481 ctcgatcaac tcggtccagc tgctcaagct ctcgggcatc ggcccggccg aggaactgaa
      541 gggcgtgggg atcgaagtcc tggccgaccg gccgggcgtg ggcgccaacc tgcaggatca
      601 cctggagttc tatttccaga tggcctgcac caagccggtc accctgttcg gcaaggccaa
      661 cctgctcggc aaggccatgg tcggggcgga atggctgttc ctgcggcgcg ggatcggggc
      721 gtcgaaccag ttcgaaagct gcggcttcat ccgcagccgg gccgggatca agtacccgga
      781 catccagtac catttcttcc cgatggcgat caattacgac ggctcttcgc tggcgacaga
      841 gcacggcttt caggctcatg tcggcccgat gcgctccaag agccgcggca cggtgaggct
      901 gcgcagcaag gacccgcgcg agcatccgaa gatcctgttc aactacatga gccacccgga
      961 cgactgggcg gaaatgcgcg cctgtgtgcg gctgacccgc gagatcttcc agcaggatgc
     1021 cttcgccccg tggcgaggcc gggaaatcca gccgggcgcg gactgcgtga gcgacgaggc
     1081 gatcgatgcc ttcatcgccg accatgtcga aagcgcgctg catccgtcct gcacctgcaa
     1141 gatgggcgat gcccgggatc cgatggcggt ggtcgatccg gaactgcggg tgatcggcgt
     1201 ggaggggctg cgggtgatcg acagctcggt catgccctcg atcaccaccg gcaacctcaa
     1261 cgcgccgacc atcatgatcg gggaaaaggg cgccgatcac gtgctgggca aggggctgct
     1321 gccgccgtcc aacgcgccct actatgttgc ccccaactgg gagatgtcgc agcgatgagc
     1381 cgcaccatta ccgaaccggc gcgggcgatc ccgatcgtcc acgaaaccga ggtgctggtg
     1441 gtcggctcgg gccccggcgg cctggcggcg gcgctggcct cggcgcgggc aggggtgacg
     1501 accaccctgg ttgagcgcta cggctgtttt ggcggcaaca tcacccaggt cggggtggag
     1561 ggctttgcct ggtaccggca cccggcgacg atcgacagcg aggggatcgg ccgcgagttc
     1621 gaggaccggg ccaaggcgat gggcgcggcc ctgccggaac cgcaatcgat cagccacgcg
     1681 ctcgatgccg aagggttcaa gtacgtcgcc gattgcctgg tggaagaggc cggggtgatc
     1741 ccgatgctcc accggatgtt cgtggccccg atcatggaag gcgatacgat ccgcggcgtc
     1801 atcgtggaga gcaaggccgg ccgcgaggcg atcctggccc agcgggtgat cgacgccagc
     1861 ggcgatgccg acatcgccca ccgcgccggc gcgccgaccc atatgcaccc caaggccgag
     1921 atgatgagcg tctcggtgat gttctcgatg tgcggggtca acaagggccg ctttatcgac
     1981 gcggtgaagg ccgatccgca gacctatgcc gattgggcca aggacggcga atggtctgtc
     2041 accacttc
//
LOCUS       DASUDV010000433         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1450612,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000433 DASUDV010000000
VERSION     DASUDV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450612"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFV30_09415"
     CDS             <1..348
                     /locus_tag="VFV30_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848351.1"
                     /translation="DKAVVDSPRHLLHGEVAYDDGRLIGRLAAGYMSKRYFTYTNDKW
                     VDGRAIVDAMIGYRLEMFGGKKVELQLNATNLFDLAYVSTIGSNGFGNSGDNQTLLAG
                     APRQIFATVKLEF"
     gene            564..2120
                     /locus_tag="VFV30_09420"
     CDS             564..2120
                     /locus_tag="VFV30_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX4848352.1"
                     /translation="MASGDPDSGSVVLWTRVTASGSLDVAWELATDRHFADIVRRGTF
                     ATGPERDHTVKVLAEGLLPGARYFYRFHANGQVSPTGQTRTLPIGSLDRLGIALVSCS
                     NYALGHFNAYAAIARDPAIDFVLHTGDYIYEHGADGWGGELARRIGRVHQPAHEIVSL
                     TDYRTRHAQYKRDAGSLAMLAAKPILACWDDHESANNPWSGGAQNHQSESEGDWNVRR
                     AASIQAYFEWMPVREPSPGRSRMQFWRTWSFGDLATLSTLETRHTARARQIDYGPYKQ
                     TIRSHADALAMERDALGAPGRLMLAPELEAELIAAWTRSKELGQPWRLIGNPMPIART
                     RVPDVVRLGLIADPARQANPRSAALELAWKGRWNLPFYPDTWDGYDWARERLYSQARA
                     AGVNDLVFLSGDSHSFWANRLADGAGRPVGIELGTAGVTSPGDFVESGFEPAIARKLD
                     EAFAEHNPEVLWTDNLHQGYVAIELRREFAKATFVAMNTLQRPDWRHFPISKWRIERG
                     NDGLDLTRRG"
     gene            complement(2175..>3231)
                     /locus_tag="VFV30_09425"
     CDS             complement(2175..>3231)
                     /locus_tag="VFV30_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX4848353.1"
                     /translation="GLLFVVPGALVILALSVLYAFAARLDWFAALFLGIKAAVLAIVV
                     QALLRIAGRALDTGLKRVLAVLAFVALFLLDLPFPLVVLGAGLIGTIVAAIRPEWLAI
                     KLRQHAEPGAPRPWRQSLQTVLAWGVIWAAPMVLVLATLGRDHVLWQIGSFFSQLAVV
                     TFGGAYAVLAYMAQEAVQGFGWLSAGEMADGLGLAETTPGPLIMVTQFVGYLAAFRAP
                     EPFTPAVAGLLGAALTTWVTFAPCFLWIFTFAPWIERMENAPRLKGGLAALTASVVGV
                     IANLTLWFVLHVLFAQVSEVRAGPVRLYSPEWTSFDWRAGVLAALAAILIFRLRWNVI
                     AVLSAAGCGGLLLGLAI"
BASE COUNT          618 a         1079 c         1043 g          491 t
ORIGIN      
        1 gacaaggcgg tggtcgattc gcccaggcat ctgctgcacg gggaagtcgc gtacgatgac
       61 ggccgcctga tcggacggct tgccgccggg tacatgtcga agcgctattt cacctacacc
      121 aacgacaagt gggtggacgg ccgggcgatc gtcgatgcga tgatcggcta ccgcctggag
      181 atgttcggcg gcaagaaggt cgagctgcaa ctcaacgcca cgaacctgtt cgatctggcc
      241 tatgtctcga cgatcggatc gaacgggttt ggcaacagcg gcgacaacca gacccttttg
      301 gctggcgccc cgcgccagat ctttgccacc gtcaaactcg aattctgagc cgccagaaat
      361 cagtccctcc ctgggggcgg ttgccatatg gtgaccgccc cgattctttg aactgccaaa
      421 ggaactgcac gatgtccggc cagtcaccga ttacccgccg ttcaacgctg gcggggatgt
      481 ccgcaacggc agctgcgctc gctgcatcac cgataccggc catggcgcaa ggttcatcgg
      541 cggattcgcc attccgtcac ggcgtggcga gcggcgatcc cgactccggc agtgttgtgc
      601 tgtggacgcg ggtgacggca agcgggtcgc tcgatgtcgc atgggagctg gcaactgacc
      661 ggcactttgc cgatatcgtc cggcgcggca ccttcgcgac cggaccggaa cgcgaccaca
      721 cggtcaaggt tctggccgag gggttgttgc cgggcgcacg gtatttctac cgctttcatg
      781 ccaatggcca ggtttcgcca accgggcaga ccaggacctt gccgatcggc tcgctcgatc
      841 ggctcgggat cgccctggta tcctgctcga actatgccct ggggcatttc aacgcctatg
      901 cggcaatcgc ccgtgatccg gcgatcgatt tcgtgctcca tacgggcgac tacatttacg
      961 aacacggcgc ggatggctgg ggcggcgaac tggccaggcg catcggccgg gttcaccagc
     1021 ccgcgcatga aatcgtctcg ctgaccgatt atcgcacgcg tcacgctcag tacaagcgcg
     1081 atgccggttc gctcgccatg ctcgccgcca agccgatcct ggcctgctgg gatgaccacg
     1141 aaagcgcgaa caatccctgg agcggcgggg cgcagaatca ccagagcgaa agcgagggtg
     1201 actggaacgt gcgccgggcc gcatcgatcc aggcctattt cgaatggatg ccggtgcgcg
     1261 aaccatcgcc aggtcgctcc cggatgcagt tctggcgaac ctggagcttt ggcgaccttg
     1321 ccacgctctc gacgctcgaa acccggcata ccgcgcgggc gcggcagatc gactatggcc
     1381 cttacaagca gacgatcagg agccacgccg atgcgctggc gatggagcgg gacgcgctgg
     1441 gcgcgccggg acgcctgatg ctggcgcccg agctcgaggc agaactgatc gcggcctgga
     1501 ccagatccaa agagctcggc cagccctggc ggttgatcgg caatcccatg ccgattgcgc
     1561 gcacccgggt ccccgatgtg gtcaggctcg gactgattgc ggacccggcc cggcaggcca
     1621 atccccggtc ggcggctctc gagctggcct ggaaaggaag gtggaacctt cccttctatc
     1681 ccgatacctg ggatggctat gattgggccc gcgagcggct ttacagccag gcacgcgcgg
     1741 caggcgtcaa tgacctggtc tttctttccg gcgacagcca cagtttctgg gccaaccgtc
     1801 tcgcggacgg cgccggacgg ccggtcggga tcgaactcgg aacggccggg gttacttcgc
     1861 ccggagactt tgtcgaaagc ggcttcgaac cggccatagc ccgcaagctc gatgaggcct
     1921 ttgccgaaca caatcccgaa gtgctctgga ccgacaacct gcaccagggc tacgtcgcga
     1981 tcgaactgcg gcgtgagttc gccaaggcaa ccttcgtggc catgaacacg ctgcaacggc
     2041 ccgactggcg ccattttccg atcagcaaat ggcggatcga gcgcggcaat gatgggctcg
     2101 atctgacacg ccggggatga tccgaccgcg cccaaggtgt gcttgcgcgg ggcctggccg
     2161 cccggcgccg gcgatcagat cgccaaaccc aggagcaggc cgccacagcc cgccgcgctc
     2221 agaacggcaa tgacgttcca gcgcaggcgg aagatcagga tcgcggcgag tgccgccagc
     2281 acccccgcgc gccagtcgaa gctcgtccat tccgggctgt acagcctgac tggcccggcc
     2341 cggacctcgc tcacttgcgc gaacaggacg tgcagcacga accacagggt caggttggca
     2401 atcacgccca cgaccgatgc ggtcagcgcg gcgaggccac ccttcaggcg gggcgcgttt
     2461 tccatccgct cgatccacgg cgcgaaagtg aagatccaca ggaagcaggg cgcgaaggtg
     2521 acccaggtgg tcagcgccgc gcccagtagc cccgccaccg cgggggtaaa cggctctggc
     2581 gcgcgaaagg cggcaagata gccgacgaac tgcgtgacca tgatcagcgg cccgggcgtg
     2641 gtttcggcca ggccaaggcc atcggccatc tccccggccg aaagccagcc gaagccctgt
     2701 accgcttcct gggccatata ggcaagtacc gcataggccc cgccgaaggt cacgactgca
     2761 agctgcgaaa agaagctgcc gatctgccac agcacgtgat cgcgcccgag cgtggcaagc
     2821 accagcacca tcggcgcggc ccagatcact ccccaggcaa gcaccgtctg cagcgattgc
     2881 cgccagggtc gcggcgcgcc gggctcggca tgctgcctga gcttgatcgc cagccattcg
     2941 ggccggatcg cggcgacgat cgttccgatc aggcctgcgc ccagcacgac cagcgggaac
     3001 ggcaagtcga ggaggaacag cgcaacaaag gccagcacgg ccagcacccg cttcagcccg
     3061 gtgtcgagcg cgcgaccggc aatgcggagc agcgcctgga cgacaatcgc cagcaccgcg
     3121 gccttgatcc ccaggaacag cgcggcaaac cagtcgagcc tggcggcgaa ggcataaagc
     3181 accgacaggg ccaggatcac cagcgcgccg ggcaccacga acagcagccc c
//
LOCUS       DASUDV010000434         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1296259,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000434 DASUDV010000000
VERSION     DASUDV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296259"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..256
                     /locus_tag="VFV30_09430"
     tRNA            167..256
                     /locus_tag="VFV30_09430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:201..203,aa:Ser,seq:cga)
     gene            complement(330..1229)
                     /gene="rfbA"
                     /locus_tag="VFV30_09435"
     CDS             complement(330..1229)
                     /gene="rfbA"
                     /locus_tag="VFV30_09435"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX4848354.1"
                     /translation="MKGIVLAGGSGRRLQPATAVVNKQLMPVYDKPMIYFPVSVLMLA
                     GIREILIVTTAQHLESFRTLFGDGRKFGLAIDYAVQPRPEGLVQAFRLGADFIAGGPS
                     ALMLGDNILYGAGLQAMLARARNRADQGVATVFGYQVDNPRAYGVVELGEQDRVLSIE
                     EKPARPRSNLAVPGLYFYDARAVDFARRVNPSDRGELEITDLNRLYLEADSLHVERIG
                     RGYAWLDAGTHDSLLEAGEFVRTVQNRQGIQLACLEEIAWQNGWIDDAALRAQAHAYA
                     ETGYGRYLSGLLPHEAAARELPD"
     gene            complement(1284..2333)
                     /gene="rfbB"
                     /locus_tag="VFV30_09440"
     CDS             complement(1284..2333)
                     /gene="rfbB"
                     /locus_tag="VFV30_09440"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX4848355.1"
                     /translation="MITGGAGFIGSALVRQLLRDTAAHVCTVDVLTYAGNLDALAEVL
                     PSDRHSFHRADIGDAEAMAGIFAAFRPDCVMHLAAETHVDRSIDRPLDFVRANLVGTA
                     TLLEAATAHWRALAGEDRARFRFLHVSTDEVFGSAGPAGSFDEASGYDPRSPYAATKA
                     GSDHLVRAWHHTYGLPAVITNCSNNFGPFQFPDKLIPRTIVRALRGETLQVYGDGANV
                     RDWLYVDDHAGALRLIMDGGRDGETYCIGGSGERSNLQVVEAVCAEMDRQRPRADGQS
                     YLTQLEFVADRPGHDFRYAVNAGKLIRELGWRPSESFESGIARTVRWNLDNEEWWRGM
                     TEGSYRGERLGLGHA"
     gene            complement(2366..2941)
                     /gene="rfbC"
                     /locus_tag="VFV30_09445"
     CDS             complement(2366..2941)
                     /gene="rfbC"
                     /locus_tag="VFV30_09445"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX4848356.1"
                     /translation="MIRTASIPFRHVPARHHDERGWFSELYSRKAMAECGVGHDFVQD
                     NFSHSVRRGTIRGIHFQRPPHGQAKLVRCLAGAILDVAVDLRAGSPSYGQWVSAVLTS
                     EGGEQLYIPEGFGHGFMTLTDNAAVSYLASDHYHPDLEGGIAWDDSDLSIAWPLAGLT
                     PTLSPKDRSLPPLRDIVSGFAYDGVPLAPLE"
     gene            3118..4239
                     /locus_tag="VFV30_09450"
     CDS             3118..4239
                     /locus_tag="VFV30_09450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4848357.1"
                     /translation="MTPSASPQRVSAPAGKVFSPKIRTFSLGPASPAVSVVMTTWNDL
                     RFIRDAVGGVLRQTFTDFEFIIVDDGSDYPHEVEALAALDPRIRIVRLDANLGAAEAG
                     NRGIALARAPIVARMDYDDIAEPGWLAAVTQALAEDPELGIVGTWATLISEHGDPLGI
                     DRTPQSDFAIRLTLLSHNPFYHSSVAYRRELIALVGGIEGGQNLTHDHRLWRALLPHC
                     RARNLPQPLVRYRYNPRGITGSSDPLTSRSRSHDIRVGLWNELGLAYPLDSRPLGDAF
                     DSFLRDRPAPHPELWGAVAEVIESAIGGLAAQEGQFLRPGDEPLRDAFVLSLRARLAA
                     GPKRPPSRVQRLRTALRLRGFRRTVAAIVRKSRNAMGAR"
     gene            4236..>4436
                     /locus_tag="VFV30_09455"
     CDS             4236..>4436
                     /locus_tag="VFV30_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX4848358.1"
                     /translation="MNVLITGANGLVARALAASVPAGVRVTGLTRQDLDICDAERVLQ
                     VVAGMQPDAVINAAAYTACDRAE"
BASE COUNT          741 a         1487 c         1470 g          738 t
ORIGIN      
        1 cggtccattg ctgccaacat aggggttgtg cgcctgcgaa acaacggctt gcagcgggcc
       61 gtcccccaaa aagggcggcg agggggcttg gcaagcgccc cgccatcggc taaccgcgcg
      121 gctcttcgac ccgaccgggc cctgcccggc gtgtgatcgc ttctgcggag aggtggcaga
      181 gaggtcgaat gcgccgcact cgaaatgcgg tttacgggca accgtaacgt gggttcgaat
      241 cccaccctct ccgccacaaa cctcccgaaa gggccagacg ttccgatcac gcacaggccg
      301 cgagcggctg gcggtcggtc gtcgctgcgc tagtccggca actctcgggc ggccgcctca
      361 tgcggcagca ggccgctgag gtagcggcca tagccggtct cggcataggc atgggcctgc
      421 gcgcgcaggg cggcgtcatc gatccagccg ttctgccagg cgatttcctc aagacacgca
      481 agctgaatgc cctggcggtt ctgcaccgtg cgcacgaact ctccggcttc aagcaggctg
      541 tcgtgcgtgc cagcatcgag ccaggcatag ccgcgtccga tgcgctcgac gtgcagactg
      601 tccgcttcaa ggtacagccg gttgaggtcg gtaatctcaa gctcgccgcg gtccgaggga
      661 tttaccctcc gcgcgaaatc gacggcccgc gcgtcataga aatagagccc tggcaccgcg
      721 agattggatc ggggccgcgc cggcttctcc tcgatcgaga gcacccggtc ctgctcgccc
      781 agctcgacca cgccgtaagc gcggggattg tcgacctggt agccgaaaac ggttgccacg
      841 ccctggtcgg cccggtttcg cgcccgagcc agcatggctt gcaggccggc gccatacagg
      901 atgttgtcgc ccagcatcag cgcgctgggc ccgcccgcga tgaaatcggc accaaggcgg
      961 aaggcctgga ccaggccttc cggccggggc tgcaccgcat aatcgatcgc caggccgaac
     1021 ttccggccat ccccgaaaag ggtgcggaag ctctccaggt gctgcgcggt ggtaacgatc
     1081 aggatctcgc ggatcccggc cagcatcagg accgacaccg gaaagtagat catcggcttg
     1141 tcgtaaaccg gcatcagctg cttgttgacg accgcggtgg ccggctgaag gcgccggccc
     1201 gagccaccgg ccaggacgat ccccttcatg accaggccgc cgccagcgcc cggccggatc
     1261 gggcgcgcat cggctgtgcc gcgtcacgca tggcccaatc ccagcctttc gccccgatag
     1321 gatccctcgg tcatgccgcg ccaccactcc tcgttgtcca ggttccagcg gaccgttcgg
     1381 gcaatcccgc tttcgaacga ctcgctcggc cgccagccca gctcgcggat caacttgccg
     1441 gcattcaccg cataacggaa atcatgaccc ggccggtcgg cgacaaattc cagctgcgtt
     1501 agatagcttt gcccgtcggc gcggggtctc tgccggtcca tttcggcgca aacggcctcc
     1561 accacctgca ggttggaccg ttccccgctg ccgccaatgc aataggtttc gccgtcccgc
     1621 cccccatcca tgatgagccg cagcgcgccg gcatggtcat cgacgtaaag ccaatcccgg
     1681 acattcgctc catccccgta gacttgcagg gtctctcccc gcaacgcccg gacgattgtc
     1741 cgcgggatca gcttgtccgg gaactgaaac ggtccgaaat tgttcgagca gttggtgatg
     1801 acagcgggca ggccataggt gtgatgccag gcccgaacca ggtgatcgct gcccgccttg
     1861 gtcgcggcat agggcgagcg tgggtcataa cccgaggctt cgtcaaacga cccggccggg
     1921 ccggcggagc caaagacctc gtcggtcgaa acatgcagaa accggaagcg cgcgcggtct
     1981 tcgccggcca gcgcgcgcca atgggctgtc gccgcttcca gcagcgtggc ggttccgacc
     2041 aggttggcgc gcacgaagtc cagcggccgg tcgatcgagc ggtccacgtg cgtttccgcg
     2101 gcaaggtgca tgacgcagtc cgggcgaaac gctgcgaaga tgccggccat ggcttcggcg
     2161 tcgccaatgt cggcgcggtg aaagctgtgc cggtccgacg gcagcacctc tgccagcgcg
     2221 tccagattgc cggcataggt gagcacatcg acggtgcaga catgcgcagc ggtatcgcgc
     2281 agcaactggc gcaccagcgc ggaaccgatg aacccggctc cccccgtaat catgacgcgc
     2341 gcgtgccgca tgggataagc ttcgatcact ccagtggggc gagaggaacg ccgtcatagg
     2401 caaagccgct gacgatgtcg cgcaagggcg gaaggctgcg gtccttgggc gacagcgtcg
     2461 gggtcagacc ggcaagcggc caggcgatgc tgagatcgct gtcgtcccag gcgatcccgc
     2521 cctcaaggtc ggggtgatag tgatccgaag ccagatagga aaccgctgcg ttgtcggtca
     2581 gggtcatgaa gccatggccg aagccctcgg ggatatagag ctgttcgccc ccttcggacg
     2641 tcaggacggc cgacacccac tgcccatagc tgggtgagcc ggcgcgcaga tccaccgcga
     2701 cgtccaggat cgctccggcc agacagcgga ccagcttggc ctgtccgtgg ggcggcctct
     2761 ggaaatggat gccccggatg gttccgcgcc ggaccgaatg gctgaaattg tcctgcacga
     2821 aatcatggcc tacgccgcac tcggccatgg ccttgcggct gtagagctcg ctgaaccagc
     2881 cgcgctcgtc gtggtgccgc gcggggacat ggcggaatgg gatcgatgcc gttcggatca
     2941 tttcgccctc ttcaaccggt tggaccgcgc ggcgccagcc ggaacggggg gaggatatgg
     3001 ccgcgcccgg cctgagtcca caagcgcatt gcctggccat gcacccgcgg atcgggcggg
     3061 cggaattgcg ttgatcgccg gcatccgcgt gcctaggctg cgccagcaaa ggtccctatg
     3121 acaccgtccg catcgccaca gcgcgtttcc gcgcctgccg gaaaggtctt cagtccgaag
     3181 atccggacct tcagccttgg cccggcaagc ccggcggttt cggtggtaat gacgacctgg
     3241 aacgacttgc ggttcatccg tgacgcggtt ggcggcgtcc tgcgccagac ctttaccgat
     3301 ttcgaattca tcatcgtcga cgacggatcg gactatccgc acgaagttga ggcgcttgcg
     3361 gcgcttgatc cgcggataag gatcgtgcgg ctcgatgcca atctcggcgc ggccgaggcc
     3421 ggcaaccggg gcatagcgct ggcccgcgca ccgattgtcg cccggatgga ctacgacgac
     3481 atcgccgaac cgggatggtt ggccgcggtc acccaggcgc tggccgaaga cccggagctt
     3541 gggattgtgg gcacctgggc cacgctgatc tcggaacacg gcgatccgct gggcatcgac
     3601 cgcaccccgc aaagcgactt tgcgatccgg ctgaccttgc tgtcgcacaa tcccttttac
     3661 cattcgtcgg tggcgtaccg ccgcgagctg atcgcgctgg tcggcgggat cgagggtgga
     3721 cagaacctga cgcacgatca ccggctgtgg cgggcgcttt tgccgcattg ccgggcccgc
     3781 aatctgccgc agccgctggt gcgttaccgc tataacccgc gcggcatcac cggcagttcc
     3841 gatccgctga ccagccgttc gcgttcgcac gatatccggg tgggattgtg gaacgaactg
     3901 gggctggcct atccgctcga ttcccggccc ctcggcgatg ccttcgacag ctttctgcgc
     3961 gatcgtccgg ctccccatcc ggagctttgg ggtgccgttg ccgaggtgat cgaaagcgca
     4021 atcggcgggc ttgccgcgca ggagggccag ttcctgcggc ccggcgacga gccgctgcgg
     4081 gatgccttcg tcctgtccct gcgggcgcgc ctcgcggccg gaccgaagcg gccgcccagc
     4141 cgggttcagc ggctgcggac ggcgctgcgc ctgcgcggtt tccggcggac cgtggccgcg
     4201 atcgttcgca aatcccgcaa cgcgatgggc gcgcggtgaa cgttcttatc accggtgcga
     4261 acggcctggt cgccagagcc ttggcggctt cggtccccgc gggggtccgc gttacgggtc
     4321 tgacgcgcca ggatcttgat atctgcgacg cggagcgggt gctgcaggtt gtggccggga
     4381 tgcagcccga tgccgtgatc aacgcggccg cctataccgc gtgcgaccgg gcggaa
//
LOCUS       DASUDV010000435         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1494724,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000435 DASUDV010000000
VERSION     DASUDV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494724"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..787)
                     /locus_tag="VFV30_09460"
     CDS             complement(101..787)
                     /locus_tag="VFV30_09460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848359.1"
                     /translation="MNLSSLDRPVWSMLTGRQAHLALGDQRAWRIDPGYGPFAAARDD
                     SAEAQGALAALAALGQPIWLVEAQAMAAPPGMQVVRTAPLLQMIAPDPAPLQPGDEEC
                     IALGDAHAEAMRDLALATEPGPWGDKTRLYGGFYGIFRDGRLAAMAGTRMRPAPGIAE
                     VSGVCTWPEFRGQGLAARLIRRVMADFAAAGEMPFLHSYAGNAKAIGLYQTLGFAARR
                     EMVVTVLECG"
     gene            complement(784..1098)
                     /locus_tag="VFV30_09465"
     CDS             complement(784..1098)
                     /locus_tag="VFV30_09465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX4848360.1"
                     /translation="MEEFDPAQPLYGLIGQMKAVPGKRDELIGYLQAGTRTMPGNLAY
                     LIAKDAADADSIWITEVWKDQASHQASLTLPGVQAAIAKARPILAGFGTRVETIPMWN
                     PA"
     gene            complement(1170..>2419)
                     /locus_tag="VFV30_09470"
     CDS             complement(1170..>2419)
                     /locus_tag="VFV30_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX4848361.1"
                     /translation="VNPRGHRALLDRYCGGEADGVAAAWTTWQAAQGALDAAREAIAR
                     AASDRDLLLAHLAELTGLEPQEGEEEELALQRATMQKGERLTDDLAALRALWAGSDSA
                     LASLRSAARRLDRIANEHPLLGEALASLDRAVIEASEAEDRLERAAEALAHDPAALDR
                     IETRLFDLRAAARKHSCQVDDLPRIMREMRGQLEGIEAGEEHLAALERAAHEAGLEYR
                     ARAEALSALRVEAAQRLDVAVAGELAPLRLDAARFRTAITRLSEEQWGAAGIDRVEFL
                     ISTNPGADFAPLAKIASGGELSRFILALKVALAEQGGAATVIFDEIDRGVGGAVASAI
                     GERLARLASGGQLLAVTHSPQVAARGGQHYLIAKSSEGTVTRTSVHQLDGAGRKEEIA
                     RMLSGAEVTAEARAQADRLLEGV"
BASE COUNT          369 a          877 c          794 g          379 t
ORIGIN      
        1 cccccaaccc cctcctctga agaggagggg gcgtcacacg cgcaaagact ctgctcctcc
       61 tcttcagagg agcgagaccg gggaaggtgt ccttgtttca tcaaccgcac tcgagcacgg
      121 tcaccaccat ctcgcgccgg gctgcgaaac ccagcgtctg gtaaagtccg atcgccttgg
      181 cattgccggc ataggaatgg agaaagggca tttcgcccgc ggcggcaaaa tcggccatga
      241 cccggcggat cagccgcgcc gcgaggccct gcccgcgaaa ttccggccag gtgcaaacgc
      301 ccgaaacctc ggcaatcccg ggcgctggcc gcatccgggt gccggccatt gccgccagcc
      361 ggccatcgcg gaaaatgccg tagaacccgc cgtaaagccg ggtcttgtcg ccccatggcc
      421 caggttcggt ggcaagggcc aggtcgcgca tcgcctcggc atgggcatcg cccagcgcga
      481 tgcattcctc gtcaccgggc tgcagcggcg cgggatcggg ggcgatcatc tgcagcaagg
      541 gcgcggtgcg caccacctgc atccccggcg gggccgccat ggcctgcgct tcgaccagcc
      601 agatcggctg tcccagcgcc gccagtgcag ccagcgcccc ttgcgcttcg gcgctgtcat
      661 cgcgcgccgc ggcaaagggg ccatagcccg gatcgatccg ccaggcgcgc tgatcgccta
      721 gcgccaggtg cgcctgccgc ccggtcagca tcgaccagac cgggcgatcg aggctggaga
      781 ggttcaagcc ggattccaca tcgggatagt ttcaacccgc gttccgaacc cggccaggat
      841 cggccgcgcc ttggcgatcg ccgcctgcac ccccggcagc gtcaggctgg cctggtgcga
      901 ggcctggtcc ttccacacct cggttatcca gatcgaatcc gcatcggccg catccttggc
      961 gatcaggtag gccagatttc ccggcatggt ccgcgttccg gcctgcaggt agccgatcag
     1021 ttcgtcgcgc ttgccgggga cggccttcat ctggccgatc agtccataga gcggctgggc
     1081 gggatcaaat tcttccatgg gtgttgcaca tccttgcaca agcagcgcca aggcggcgct
     1141 gccgatgaat tcacggcggg gcaagtggct cacaccccct ccagcagacg atcggcctgg
     1201 gcgcgcgcct ctgcggtcac ttccgcgccg gacagcatcc gggcgatctc ttccttgcgg
     1261 cccgcaccat cgagctggtg caccgaggtg cgggtgaccg tgccctcgct cgacttggcg
     1321 atcaggtagt gttgcccgcc gcgcgcggcg acctgcggcg agtgggtgac cgcaagcagc
     1381 tggccgccgc tggccagccg cgccagccgt tcgccgatcg ccgaggcgac cgcgccgccc
     1441 acgccgcggt cgatctcatc gaagattacg gttgccgccc cgccctgttc ggccagcgca
     1501 accttgagcg cgaggatgaa gcgcgacagt tcgccgcccg atgcgatctt ggccagcggc
     1561 gcgaaatccg cgcccggatt ggtgctgatc aggaattcca cccggtcgat cccggccgcg
     1621 ccccattgtt cttcgctcag gcgggtgatc gcggtgcgga accgggcggc atcgagcctc
     1681 agcggggcaa gttcacccgc caccgccaca tcgagccgct gcgccgcttc gacacgcaag
     1741 gccgaaagtg cctcggcccg ggcgcgatat tcaaggccgg cttcgtgtgc ggcgcgttcg
     1801 agcgcggcaa ggtgttcctc gcctgcctct attccttcca gctggccgcg catctcgcgc
     1861 atgatccggg gcagatcatc gacctggcag gagtgcttgc gcgcggcggc gcgcagatcg
     1921 aacagccggg tttcgatccg gtcgagcgcg gcgggatcgt gggccagcgc ttcggccgcg
     1981 cgctcaagcc ggtcctcggc ctcgctcgcc tcgatcaccg cccgatcgag cgatgccagc
     2041 gcctcgccca gcagggggtg ttcgttggcg atccggtcaa gccgccgggc cgcgctgcgc
     2101 aaggacgcca gcgccgaatc cgaacccgcc cacagcgccc gcagcgcggc gagatcgtcg
     2161 gtcagccgct cgcccttctg catcgtcgcg cgctgaaggg cgagttcttc ctcctcccct
     2221 tcctggggtt caagtccggt cagctcggca aggtgggcca gcagcagatc gcgatccgaa
     2281 gcggcccggg cgatggcttc gcgcgccgca tcgagcgcgc cttgcgcggc ttgccaggtg
     2341 gtccaggccg cggccacacc atctgcctcg cccccgcaat agcgatcgag caaggcacga
     2401 tgcccgcgcg ggttgacca
//
LOCUS       DASUDV010000436         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1671622,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000436 DASUDV010000000
VERSION     DASUDV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671622"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFV30_09475"
     CDS             <1..770
                     /locus_tag="VFV30_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4848362.1"
                     /translation="SGAPTQELPVGADGLVDAEADFAPGIGLVAVMQVNNEIGTIQPV
                     ERLAERAHEIGALFLCDAVQGAGKLAPPEGADLIAITAHKLYGPKGIGALWVRDGVKI
                     GPLIHGGGQEGGLRSGTLSPALCAGFGAAAALAKERMEADFQHLSGLAALARQILSRW
                     TVNGSETARWPGNLNLRLDGLNFPRLLSDCRNLSFSAGSACGSGTGAPSRVLSAIGLD
                     AKEARNSIRLGFGRYTTREEVETACTAIEAAAAAQGV"
     gene            770..1102
                     /locus_tag="VFV30_09480"
     CDS             770..1102
                     /locus_tag="VFV30_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4848363.1"
                     /translation="MVKVTFVSPNGDRTKAEGAAGLRLLEVGQNAGMPLEGTCEGQMA
                     CSTCHVIVARDWFDRLVPASEDEEDMLDLAAGVTRTSRLACQIELTAELDGLEVRIPG
                     ESHDMQRR"
     gene            1134..1853
                     /locus_tag="VFV30_09485"
     CDS             1134..1853
                     /locus_tag="VFV30_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848364.1"
                     /translation="MKTRNLWLGASLSFLASAGIAGIPTSDKLTCPVGGKSFSVTGTA
                     SCSTFGATQDFFLKVETSCDFVTRLPQCPDNGLPLYKEFTKDEISLLKDYVATPEYTA
                     LAGRSRFAIAKKVDDFLVSKGSKSAFGFWYVLGGLQYDREATLSDPEYMGWLQEKGAE
                     ALTTAKGVDAAVIRLVMAYGRYLSGDFEGASAGLAAVRADAEVKDHWLVAAYAARLEP
                     CIAARDVKLCPPDMRIKPDAR"
     gene            complement(1850..>2254)
                     /locus_tag="VFV30_09490"
     CDS             complement(1850..>2254)
                     /locus_tag="VFV30_09490"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX4848365.1"
                     /translation="VLRDRLLGLLARHNGSVAELIEAERGVAAVNEEIDEAQRTLKEM
                     NGRIEFSQVSIDYDNSSASATGFFEPIRAVLDSFGAILGFIIACLIVITIAILPVTVF
                     AMAWRWAWRRGRALLQRFSGPAQPVAAVEDQP"
BASE COUNT          369 a          748 c          791 g          346 t
ORIGIN      
        1 ccagcggtgc gccgacgcag gaactgccgg tgggcgccga tggcctggtc gatgccgagg
       61 cggacttcgc ccctggcatc ggccttgtcg cggtgatgca ggtcaacaac gagatcggca
      121 cgatccagcc ggtcgagcgc ctggccgagc gtgcgcacga gatcggcgcg ctgttcctgt
      181 gcgatgcggt gcagggcgcg ggcaagctgg ccccgccaga gggagcggac ctgatcgcga
      241 ttaccgcgca caagctctat ggccccaagg gcatcggcgc gctgtgggtg cgcgacgggg
      301 tgaagatcgg cccgctgatc cacggcggcg gacaggaagg cggcctgcgc tccggcacgc
      361 tcagccccgc gctctgcgcc gggttcgggg cggcggcggc gctggcgaag gagcggatgg
      421 aggccgattt ccagcacctt tcggggctgg ctgcgctggc ccgccagatc ctctcgcgct
      481 ggaccgtcaa cggcagcgag accgcgcgct ggcccggcaa ccttaacctg cggctcgacg
      541 ggctcaactt tccgcgcctg ctcagcgatt gccgcaacct gtccttctcg gccggatcgg
      601 cctgcggcag cggcaccggc gcgcccagcc gggtgctcag cgcgatcggg ctcgatgcca
      661 aggaggcccg caactcgatc cgcctgggct tcgggcgcta caccacccgc gaagaggtcg
      721 agacggcctg caccgcaatc gaagcggccg ccgccgcgca aggagtctga tggtcaaggt
      781 cacatttgtc agccccaacg gcgatcgcac caaagcggaa ggcgcggcgg ggctgcgcct
      841 gcttgaagtg gggcagaacg ccggcatgcc gctggagggc acctgcgaag ggcagatggc
      901 ctgctccacc tgccacgtga tcgtggcgcg cgactggttt gaccggctgg ttccggccag
      961 cgaggacgag gaggacatgc tcgatctggc cgccggcgtc acccgcacca gccggctcgc
     1021 ctgccagatc gagctgactg cggaacttga cgggctggaa gtgcgcattc ccggcgaaag
     1081 ccatgacatg cagcggcgct gagcgcagcg ccaagacgga aggggacagg atcatgaaga
     1141 cgcgcaatct gtggctcggg gcctcgctgt ccttcctcgc cagcgccggg atcgcgggca
     1201 ttccgacgtc cgacaagctg acctgcccgg ttggcggcaa gtccttttcg gtcaccggga
     1261 cggcatcctg cagcaccttt ggcgcaacgc aggacttttt cctgaaagtg gagaccagct
     1321 gcgatttcgt gacccggttg ccgcagtgcc cggacaacgg gctgccgctt tacaaggagt
     1381 ttaccaaaga cgagatctcg ctgctgaaag actacgtcgc caccccggag tacaccgcgc
     1441 tggccggccg ttcgcgcttt gcgatcgcca agaaggtcga cgatttcctg gtcagcaagg
     1501 gcagcaaatc ggccttcggc ttctggtatg tcctgggcgg cctgcaatac gaccgtgagg
     1561 cgacgctcag cgatcccgaa tacatgggct ggcttcagga aaagggcgcc gaggcgctga
     1621 ccacggcgaa gggcgtcgac gccgccgtga tccggctggt tatggcctat ggccgctatc
     1681 tgtccggcga tttcgaaggg gcgagcgccg ggctggccgc cgtgcgggcg gatgcggagg
     1741 tcaaggacca ctggctggtc gccgcctatg ccgcgcggct ggaaccgtgc atcgcggcgc
     1801 gcgacgtgaa gctgtgccct ccggacatgc ggatcaagcc ggacgcgcgc tagggctggt
     1861 cttccaccgc tgccacgggc tgcgctgggc cgctgaagcg ctgcagcagc gcgcggccgc
     1921 gccgccaggc ccagcgccag gccatggcga acaccgtgac cggcaggatc gcgatggtga
     1981 ttacgatcag gcaggcgatg atgaagccca ggatcgcgcc gaagctgtcc agcaccgccc
     2041 ggatcggctc gaaaaagccg gtggccgaag cgctggagtt gtcatagtcg atcgacacct
     2101 ggctgaattc gatccggccg ttcatctcct tcagcgtccg ttgggcctcg tcgatctcct
     2161 cgttgaccgc ggccactccg cgctccgcct cgatcagttc ggcgaccgag ccgttgtggc
     2221 gggccagcag gcccagcagc cggtcgcgca gcac
//
LOCUS       DASUDV010000437         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2069371,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000437 DASUDV010000000
VERSION     DASUDV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069371"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFV30_09495"
     CDS             complement(<1..364)
                     /locus_tag="VFV30_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4848366.1"
                     /translation="MDSPVLYSLDADGIATITLNRPELRNPISDLDLIEALLDTLRGM
                     EEDPKVRVAILTGAGSAFSAGGNLKAMLPDSGGLNASLPARTRRNYKSGIQRLPLAFA
                     ALEVPVIAAVNGPAIGAGL"
     gene            complement(384..1358)
                     /locus_tag="VFV30_09500"
     CDS             complement(384..1358)
                     /locus_tag="VFV30_09500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4848367.1"
                     /translation="MSETRRLHGLDAMRGLAALAVVGFHAHDTFDAFPAWWSKGYLAV
                     DFFMVLSGYVMARTYEVRMASGLSARTFLTMRYRRLWPTMAIGGLIGIPYLWWVAGDP
                     VWFALALVLNLALLPAPLNAQAFPLNLPAWSVFYELVANLIHSLFLHKLDNAKLLIVI
                     AALLSVMSYLAHFKGDVDFGAFSEQFVFAILRCLLPYSIGVLLWRLWQDEPTLKVPPA
                     LAIAALPVLLLSPFAALGWFYDIAFIALACPLLIAGGLRMQGAPRWAVWSGLLSFPLY
                     AVHMPVLRLGEMYGIDPLLITALAVLAGALAAWWLSARSRRAKAPVTT"
     gene            complement(1355..2341)
                     /locus_tag="VFV30_09505"
     CDS             complement(1355..2341)
                     /locus_tag="VFV30_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX4848368.1"
                     /translation="MILRRLFLALALLSAWIALPARADELRPGYLELTQLDAPTWKLV
                     WKAPVLGGLATRTRPACPDFCQQSPPQARVEGLVLVAESRLTCSKDLAGSQVGLSGMD
                     AAFTDALLRVAPLNRPVQAARLTQDTAMVEVATVPDGWEVARSYFVLGIIHILEGYDH
                     LLFVIALVLLIGRPMAVIKAASAFTLAHSITLIGSTLGLIGLAQAPVEALVALSIVFL
                     AVEIVKRDPEAPSLTERKPWLVAFLFGLIHGFGFAGALREIGLPESDVPTALLTFNLG
                     VEAGQLVIVAGVLIVLAGLRRFVPQALRPSTLTATYAIGTVSSFWFIERILG"
     gene            complement(2338..>3084)
                     /locus_tag="VFV30_09510"
     CDS             complement(2338..>3084)
                     /locus_tag="VFV30_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4848369.1"
                     /translation="LLSGRAPDLGERRIVVDERVVGGLVSRFYDSFRRMPTREETDGL
                     IRDWVQDQVYYREALVLGLDENDEVVVRRMRRKMESLAVADAETKTPSDAELEAMIAK
                     DPARYADEPRTSFEHVYLGADTPQARAEAERQLQRLHKGEAVAGVPLPIPARFAKASS
                     SDISGQFGDEFTLALRNLPTGQWSGPVASGLGLHMVRVTERGAAQPPQLAKVRQRVEN
                     DWRAAATAKAREEAYARILKGYDVVIELPK"
BASE COUNT          550 a         1028 c         1094 g          412 t
ORIGIN      
        1 ccagcccggc gccgatcgcc gggccgttga ccgcggcgat caccggcact tccagcgcgg
       61 cgaaggcgag gggcaggcgc tggatcccgc tcttgtaatt gcggcgggtg cgggccggga
      121 ggctggcatt gagcccgccc gaatccggca gcatcgcctt gaggttgccc ccggccgaga
      181 aggccgatcc ggccccggtc aggatcgcga cgcggacctt gggatcctct tccatccccc
      241 gcagcgtatc cagcagcgcc tcgatcagat cgaggtccga gatcgggttg cgcagctcgg
      301 gccggttgag ggtgatcgtg gcaatgccat cggcatcgag cgaatagagg acggggctgt
      361 ccatggtggg gctctccaga aagtcaggtc gtgacgggcg ctttggcacg ccggctgcgg
      421 gccgacaacc accaagcggc cagcgcgccg gccagcactg ccaaggcggt gatcagcagc
      481 ggatcgatcc catacatctc gcccagccgc agcaccggca tgtggacggc atagagcggg
      541 aacgacagca ggcccgacca caccgcccag cgcggcgcgc cctgcatccg caggccgccc
      601 gcgatcagca gcggacaggc gagcgcgatg aaggcgatgt cgtaaaacca gcccaacgcg
      661 gcaaagggcg agagcagcag caccggcagg gcggcaatgg ccagggcagg ggggaccttc
      721 agcgtcggct cgtcctgcca cagccgccac agcagcaccc cgatcgaata gggcagcagg
      781 cagcgcagga tggcgaagac gaactgctcg gaaaaggcgc cgaaatcgac gtcgcccttg
      841 aagtgggcga ggtaggacat gaccgagagc agcgcggcga tcacgatcag cagcttggcg
      901 ttgtcgagct tgtgcaggaa caggctgtgg atcaggttgg cgaccagttc atagaacacc
      961 gaccaggccg gcaggttgag cggaaaggcc tgggcgttga gcggggcggg cagcagcgcc
     1021 aggttgagca ccagcgccag cgcgaaccag accggatcgc ccgccaccca ccacagatag
     1081 gggatgccga tcagcccgcc gatcgccatg gtcggccaca gccggcggta gcgcatggtc
     1141 aggaaggtcc gtgcggaaag gccgctggcc atccgcactt cataggtccg ggccatgaca
     1201 tagcccgaca ggaccatgaa gaaatccacc gcgaggtagc ctttgctcca ccacgcgggg
     1261 aaggcgtcaa aggtgtcgtg ggcgtgaaag ccgaccacgg ccagcgcggc cagaccgcgc
     1321 atcgcgtcca gcccgtgcag ccgccgcgtt tcgctcaccc caggatcctc tcgatgaacc
     1381 agaagctgga gacggtgccg atcgcatagg tggcggtcaa ggtggagggg cgcagcgctt
     1441 gcgggacgaa gcggcgcaag ccggcgagga cgatcagcac cccggccacg atcaccagct
     1501 gcccggcctc gacccccagg ttgaaggtca gcagcgcggt cggcacgtcg ctttcgggca
     1561 ggccgatctc gcgcagcgcc ccggcaaagc cgaagccgtg gatcagcccg aacaggaaag
     1621 cgaccagcca gggcttgcgc tcggtcaggc tgggggcttc gggatcgcgc ttcacgatct
     1681 ccaccgccag gaacacgatc gacagcgcca ccagcgcctc gaccggggcc tgggccaggc
     1741 cgatcagccc cagggtggag ccgatcaggg tgatcgagtg ggccagggta aaggccgagg
     1801 ccgccttgat cacggccatc ggccggccga tcagcaggac cagcgcgatc acgaacagca
     1861 ggtggtcata gccttcgagg atgtggatga tcccaagcac gaaatagctt cgcgccactt
     1921 cccagccatc aggcacagtg gcgacttcga ccatcgccgt atcctgcgtc agccgcgcgg
     1981 cctggaccgg gcggttcagc ggcgcgaccc gcagcagcgc gtcggtgaag gcggcgtcca
     2041 tgcccgagag gccgacctgc gaaccggcca ggtctttgct gcaagtcagc cggctttcgg
     2101 cgaccagcac cagcccctcg acccgggcct gcggggggct ttgctggcag aaatcggggc
     2161 aggcggggcg ggtgcgggtg gcgaggccgc ccagcaccgg ggccttccac accagcttcc
     2221 aggtcggcgc gtcgagctgg gtcagctcca ggtagccggg gcgcagctcg tcggcgcggg
     2281 ccggcagggc gatccacgca gagagcaggg caagcgcaag gaacaggcgg cgcagaatca
     2341 cttcggcagc tcgatcacca cgtcatagcc cttgaggatg cgggcatagg cttcctcgcg
     2401 ggccttggcg gtggcggcgg cccgccagtc gttctccacc cgctggcgga ccttggcgag
     2461 ctgcggcggc tgtgcggccc cgcgttccgt cacccggacc atgtgcaggc cgaggcccga
     2521 ggcgaccggg ccgctccatt ggccggtcgg aaggttgcgc agggccagcg tgaactcgtc
     2581 gccgaactgc ccggagatgt cgctggacga agccttggcg aagcgtgccg ggatcggcag
     2641 cggaaccccg gccaccgcct cgcccttgtg caggcgttgc agctggcgtt cggcctcggc
     2701 gcgggcctgg ggcgtgtccg cgcccagata gacgtgttcg aagctggtgc gcggttcgtc
     2761 ggcatagcgg gccgggtcct tggcgatcat ggcttcgagt tcggcgtcgc tgggggtttt
     2821 ggtttcggca tcggcgacgg ccagggattc catcttgcgg cgcatccggc ggaccacgac
     2881 ctcgtcgttc tcgtccagcc ccaggaccag cgcctcgcgg taatagacct ggtcctgcac
     2941 ccaatcgcgg atcagcccgt cggtctcctc gcgggtcggc atgcggcgga aggaatcgta
     3001 gaaccggctg accagcccac cgaccacgcg ttcatcgacg acgatccgcc gctcgcccag
     3061 atccggcgcg cggccggaca gcag
//
LOCUS       DASUDV010000438         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_456828,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000438 DASUDV010000000
VERSION     DASUDV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456828"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFV30_09515"
     CDS             complement(<1..873)
                     /locus_tag="VFV30_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4848370.1"
                     /translation="MKTSVLALSAFAFALSAAPALAQAQQRAMTAEDLITLKRVAAPT
                     ASPDGAVVVWQQTETDPASYKRITGLWRVPAKGGTPERVADLPDAGENSPAFSPDGKR
                     LYFLSDKSGKDQVWFLDLTAPGAAPVQASDFKTDVAGFELSPDGKRILVWGDVARDCP
                     TLGCEASGDTSQPGPGTGRHYKHGTGFVRHWDAWETPGNYSRAFALDLGADGKVAGTA
                     VALDGPPGVLTGDTPSKPMGGGEELAWAADSKSVFFTARQADAKEPLSTNLDVWHAML
                     DGKAPHSPTKANLAT"
     gene            complement(907..1362)
                     /locus_tag="VFV30_09520"
     CDS             complement(907..1362)
                     /locus_tag="VFV30_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX4848371.1"
                     /translation="MRQAGEPSVEEILDSIKKVIARDNRTAAAEQAPRREPVAPPAAE
                     VAAEDDEDDVLDLGAVDELVEDDAAAEDAPLLPRETASSISESLAALAVMAEPRAAPQ
                     IVRSGETSLEGLVREMLRPALAEWLDKNLPPLVERMVAAEIARIVGKKG"
     gene            complement(1401..>2609)
                     /locus_tag="VFV30_09525"
     CDS             complement(1401..>2609)
                     /locus_tag="VFV30_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX4848372.1"
                     /translation="DGSSGNPLAPDRNLDAQLNFGVPIYAGGGIRNSIRSAETRVIAG
                     RADLRAAETAVFSQVVAVYMDVILAESVVELNRANVKRLEVNLQATRDRFEIGDLTRT
                     DVAQSESRLAFAQGQARTAEANLISARERYIQVVGKAPENLKTPPPLVGLPGSADDAV
                     AIALESNPDLLAARQRSRAAGYDSAAAGSGRLPTVSVFANTGYNDTLGTNQIPGAVQS
                     TTSTTVGVRATVPLFQGGLPSAQRRQAQAREGALLEQEIGIERTVIATVRSAYAQWQA
                     ALSLITSSQAAVNAAELSLEGVRAENSVGNRTILDILNAENELLTAQVQLVTAKRNAY
                     VAGFNLLAAMGKAEARDLGLDGGALYDPEVNYRNARGSIWDWNDHPDPKPLSTRTVDT
                     KAQDGSVTGR"
BASE COUNT          350 a          882 c          929 g          448 t
ORIGIN      
        1 ggtcgccagg ttggccttgg tcgggctgtg cggggccttg ccgtccagca tggcgtgcca
       61 gacatccagg ttggtcgaca gcggctcctt cgcgtcggcc tgacgggcgg tgaagaacac
      121 gctcttcgaa tccgccgccc aggccagctc ctcgccgccg cccatcggtt tggacggggt
      181 gtcaccggtg agcacgccgg gagggccgtc cagcgccacg gcggttcccg ccaccttgcc
      241 gtcggcgccg agatcaaggg cgaaggcgcg gctgtagttg cccggcgttt cccaggcgtc
      301 ccagtggcgg acaaagccgg tgccgtgctt gtaatgccgt ccggtgccgg ggcccggctg
      361 gctggtgtcg ccgctcgcct cgcagcccag ggtcgggcaa tcgcgcgcga catcgcccca
      421 gaccaggatg cgcttgccgt cgggcgacag ttcaaagccg gccacatcgg tcttgaaatc
      481 gctggcctgg accggcgcgg cgccgggcgc ggtcagatcg aggaaccaga cctggtcctt
      541 gccgctcttg tccgagagga aatagagccg cttgccgtcc gggctgaagg ccgggctgtt
      601 ctcgcccgcg tcgggcaggt cggccacgcg ctcgggcgtg ccgcccttgg ccgggacgcg
      661 ccacaggccg gtgatgcgct tgtagctggc cggatcggtt tcggtctgct gccacaccac
      721 cacggccccg tcgggcgaag cggttggcgc ggccacgcgc ttcagggtga tcaggtcttc
      781 cgcggtcatg gcccgctgct gggcttgcgc cagggcaggg gcggcggaca gggcgaaggc
      841 gaacgcgctg agcgcgagga ctgaagtctt catgctgccg ggattagccg cagttgcggc
      901 ccggctctag cccttcttgc cgacgatccg ggcgatttcg gccgcgacca tccgttccac
      961 cagcggcggc aggttcttgt cgagccattc ggccagcgcc ggacgcagca tttcgcgaac
     1021 cagcccttcc agcgaggttt cgcccgagcg gacgatctgc ggcgcggcgc gcggttcggc
     1081 catcacggcc agggcggcca gcgattcgct gatcgagctg gcggtttcgc ggggcagcag
     1141 cggcgcgtct tcggccgcgg catcgtcctc gaccagttcg tcgaccgcgc cgagatcgag
     1201 cacgtcgtcc tcgtcatcct ccgccgcgac ttcggccgcg gggggtgcca caggctcgcg
     1261 ccgcggggcc tgttcggccg cggcggtgcg gttgtcccgc gcaatcactt tcttgatcga
     1321 gtcgaggatt tcctcgaccg aaggttcacc cgcctggcgc atcgcccaaa tcccccaagc
     1381 cgtcccgctg cgggacagaa tcaccgaccg gttactgaac cgtcctgcgc cttggtgtca
     1441 acggtgcgcg tcgacagcgg cttgggatcg gggtggtcgt tccagtccca gatcgagccg
     1501 cgggcgttgc ggtaattgac ttcggggtca taaagcgcgc cgccatcgag ccccagatcg
     1561 cgggcttcgg ccttgcccat tgccgccagc aggttgaacc cggcgacata ggcattgcgc
     1621 ttggccgtga cgagctggac ctgggccgtg agcaattcgt tctcggcgtt gagaatgtcg
     1681 agaatcgtgc ggttgccgac gctgttttcg gcccgcacgc cttccaggct cagttcggcg
     1741 gcattgaccg cggcctgcga gctggtgatc agcgaaagcg cggcctgcca ctgggcatag
     1801 gccgaacgga cggtggcgat cacggtccgc tcgatcccga tttcctgttc cagcagcgcg
     1861 ccttcgcggg cctgggcctg gcggcgctgg gccgaaggca ggccgccctg gaacagcggc
     1921 accgtggcgc ggacgcccac cgtggtgctg gtcgtgctct gcaccgcgcc ggggatctgg
     1981 ttggtgccca gcgtatcgtt gtagccggtg ttggcgaaga ccgagaccgt cggcaggcgg
     2041 cccgaaccgg ccgccgcgct gtcataaccg gcagcgcggc tgcgctgacg tgcggcgagc
     2101 agatcggggt tcgattccag cgcgatcgcc acggcatcgt cggcgctgcc cggcaggccc
     2161 accagcggcg gcggggtctt gaggttctcg ggcgccttgc cgaccacctg gatatagcgc
     2221 tcgcgcgccg agatcaggtt ggcttcggcg gtacgggcct ggccctgggc aaaggccagc
     2281 cggctttccg actgggccac gtcggtgcgg gtcaggtcgc cgatttcgaa ccggtcgcgg
     2341 gtggcctgaa ggttcacttc cagccgcttc acgttggcgc ggttcagttc gaccaccgat
     2401 tcggccagga tcacgtccat gtagaccgcc acgacctggc tgaagaccgc ggtctcggcc
     2461 gcgcgcagat cggcccggcc ggcgatcacc cgggtttcgg ccgaacggat cgaattgcgg
     2521 atcccgcccc cggcgtagat cggcacgccg aaattgagct gggcatcaag gttgcggtcc
     2581 ggcgccagcg ggttgccgct gctgccgtc
//
LOCUS       DASUDV010000439         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1119353,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000439 DASUDV010000000
VERSION     DASUDV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119353"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VFV30_09530"
     CDS             complement(<1..865)
                     /locus_tag="VFV30_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HEX4848373.1"
                     /translation="MFNDFYGLTGRPFQLTPDPTFYFESLTHRKALSYLSYGLAQGEG
                     FVVITGEVGAGKSTLVAHLMATIDPARLTAAQIVTSKLDGEELVHVVAQAFGLIVDGH
                     DKASALGAIEAFLHDEARAGRRCLLVVDESQNLGIDALEELRMLSNFQLGAHPLLQTL
                     LLGQPEFRDTIQDHPQLEQLRQRVIAAHHREAMELGEVQPYIEHRLKCVGWNGNPKFD
                     QRVYTELYEASGGVPRRINQICNRLMLLGAVEQRSRIDGAMLSQVLEELELDGTLQLK
                     KVTKPAPAAPAP"
     gene            complement(889..>2370)
                     /locus_tag="VFV30_09535"
     CDS             complement(889..>2370)
                     /locus_tag="VFV30_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX4848374.1"
                     /translation="DEVLTYTTVAAGIEANVNGRNTQASAAVRYERRFGYGSNRVPDT
                     DVVSGVARGSVAIVPRALTFEAGALAARTSVENNGSTLTGIETGSTSQVYGAYLGPSL
                     RTQMGELQVEGHYRFGYNRVEAPRSLVVVPGQNPVDVYDEGTQHNAALRAGFRPGTLL
                     PIGVGVGAGWNREDVSNLDQRIDDKHVRGDVIVPIGQSLALVGGIGYEDVTVSHRDAV
                     RDPLTGQPVIGSDGRYVTDKSQPRQIAYESDGLIWDAGVLWRPSKRTSLEAHVGRRYG
                     STTYYGSFVYAPTDRVSLNLSAYDNVTGFGGMLTNRLAALPTQFEAVQNPISGDLGSC
                     VAASASVGSGQSTCFGGALASVNSAVFRGRGVTGSVSVTSGSLQYGIGVGYDRRKFVA
                     APGTVLASANGVIDENLWMGAYLNGRIDRNSGYSTNIWASWYQSGDAGAGDVKAYGAT
                     AAYYRSITSRLSATAAVGVEGVDRELLQDIWTGQALVGLRYRF"
BASE COUNT          386 a          811 c          777 g          396 t
ORIGIN      
        1 ccggagccgg agcggctgga gcgggcttgg tcaccttctt gagctgcagc gtgccgtcca
       61 gttccagctc ttcgagcacc tggctgagca ttgcgccgtc gatccggctg cgctgctcga
      121 ccgcgccgag cagcatcagg cggttgcaga tctggttgat ccggcgcggc acgccgcccg
      181 aggcttcgta aagctcggta tagacgcgct gatcgaactt gggattgccg ttccagccca
      241 cgcacttcag gcggtgttcg atatagggct ggacttcgcc cagctccatc gcctcacggt
      301 ggtgggcggc gatcacgcgc tggcgcagct gctccagctg cgggtgatcc tggatcgtgt
      361 cgcggaattc cggctggccc agcagcaggg tctgcagcag cggatgcgcg cccagctgga
      421 aattggagag catgcgcagc tcttccagcg cgtcgatccc caggttctgg ctttcatcga
      481 ccaccagcag gcagcggcgg ccggcgcggg cctcgtcgtg caggaaggcc tcgatcgcgc
      541 ccagcgcgct ggccttgtcg tgcccgtcaa cgatcaggcc aaaggcctgg gctaccacgt
      601 ggaccagctc ttcaccgtcc agcttgctgg tcacgatctg cgccgcggtc agccgggcgg
      661 gatcgatcgt cgccatcagg tgcgcgacca gggtcgactt accggccccg acctcacccg
      721 tgatcaccac gaagccttcg ccctgggcca ggccatagga cagataggac agcgccttgc
      781 ggtgcgtcag gctctcgaaa tagaacgtcg gatcgggcgt cagctggaac ggccggccgg
      841 tcagtccgta gaaatcgttg aacatcatca tttcccttcc cggcaatctc agaagcggta
      901 ccgcaggcca accagcgcct ggccggtcca gatatcctgc agcaattcgc ggtcgacacc
      961 ttccaccccg accgcggccg tggccgaaag ccggctggtg atcgaccggt aataggccgc
     1021 cgttgcccca taagccttca catcaccagc accagcgtct ccgctttggt accaactggc
     1081 ccagatgttg gtgctgtaac ccgaattgcg atcgatccgc ccgttgaggt aggcccccat
     1141 ccagaggttc tcgtcgatca ccccgttggc cgaagccagg accgtgcccg gcgcggcaac
     1201 gaacttgcgg cggtcatagc cgacgccgat gccgtactgc aggctgccgc tggtgaccga
     1261 gacgctgccg gtcaccccgc ggccgcggaa cacggccgag ttgaccgagg ccagcgcccc
     1321 gccaaagcag gtgctctgcc ccgaaccgac cgaagcgctg gccgccacgc agctgcccag
     1381 atcgcccgag atcgggttct ggaccgcttc gaactgggtc ggcagcgcgg ccaggcggtt
     1441 ggtcagcatc ccgccgaaac cggtgacatt gtcataggcc gacaggttca ggctgacccg
     1501 gtcggtcggc gcatagacga agctgccgta ataggtggtc gagccatagc ggcggccgac
     1561 atgggcctcc agcgaggtcc gcttgctggg ccgccacagc accccggcat cccagatcag
     1621 gccatcggat tcgtaagcga tctggcgcgg ctggctcttg tcggtgacat agcgcccgtc
     1681 gctgccgatc accggctggc cggtcagcgg atcgcgcacg gcatcgcggt gcgaaacggt
     1741 cacgtcctcg tacccgatcc cgccgaccag ggcgaggctc tggccgatcg gcacgatcac
     1801 gtcgccgcgg acgtgcttgt cgtcgatgcg ttgatcgaga ttggagacat cttcgcggtt
     1861 ccagcccgcg ccgacgccga cgccgatcgg cagcagcgtg cccggacgga agccggcgcg
     1921 cagggccgcg ttgtgctggg tgccctcgtc atagacgtcg accgggttct ggcccggaac
     1981 gacgaccagc gagcgcggcg cctccacccg gttgtagccg aagcggtaat gcccttcgac
     2041 ctgcagctcg cccatctggg tgcgcagcga cgggccaagg taggcgccgt aaacctgcga
     2101 ggtcgaaccg gtttcgatgc ccgtgagggt cgacccgttg ttttccacgc tggtccgcgc
     2161 ggccagggcg ccggcctcga aggtcagcgc gcgcgggacg atcgccacgc tgccgcgcgc
     2221 aaccccgctg accacatcgg tatcgggcac gcggttgctg ccatagccga agcggcgttc
     2281 atagcgcacc gcggccgaag cctgggtgtt gcggccgttg acattggcct caatcccggc
     2341 ggcgaccgtg gtgtaggtca gcacttcgtc
//
LOCUS       DASUDV010000440         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_191727,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000440 DASUDV010000000
VERSION     DASUDV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191727"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="VFV30_09540"
     CDS             <1..1709
                     /locus_tag="VFV30_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX4848375.1"
                     /translation="FVLPGLIMIGWWTGFPYWLSVAVIAVGGILGVMYSVPLRRALVT
                     GSSLPYPEGVAAAEVLKVGAGVGGAEENRKGLAAIVLGSVLSAGYTLLAKMKVVAEEA
                     AVTFKLGAGATSFSTSFSLALIGVGHLVGMAVGIAMLVGLVIGWIVLLPQFTAGGLPA
                     GVELADFVSTTFREKVRFVGAGTIGVAAIWTLLKMIGPIVKGIAGALAAARARKAGAG
                     ASLDITEQDLPIGIVGGTIVVAMLPIAILLAGFAAGGPIEAMLGTTLGLSIIYVLLAG
                     VVIASVCGYMAGLIGASNSPISGVGILASLGISLLLAMIFGQGGDEATTKALVAFALF
                     VTAIVFGIATISNDNLQDLKTGQLVGATPWRQQVALILGVIFGALVIPPVLDLLNTTF
                     GFQGAPGAGEDALAAPQAALITAIAQGVLGGNLDWQLIGTGAAIGGGVVLVDELLRRS
                     GKGSLPPLGVGMGIYLPMALTLLIPVGAIMGFVYDRWARRQQDHGFAERMGVLAATGL
                     IVGESLFGVAFAGIAAATGSPEALSIVEENGWAVPMALLAFPAAVVWLYAATRKAASR
                     AG"
     gene            complement(1725..2084)
                     /locus_tag="VFV30_09545"
     CDS             complement(1725..2084)
                     /locus_tag="VFV30_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848376.1"
                     /translation="MKQALAIITVLGSGPALAVPGGMIDSLVPGTYICELPGDATGPA
                     GHRQDAESFTVLNASTYESTGGRGTYLLTGDKLSMTSGPKAGQQFRRLSANFLRKLES
                     DGKEGPLRCVRRVVNNT"
     gene            2217..>2309
                     /locus_tag="VFV30_09550"
     CDS             2217..>2309
                     /locus_tag="VFV30_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX4848377.1"
                     /translation="MAKISGVDIRPGNILEYEGGIWKVAKTQHTQ"
BASE COUNT          333 a          695 c          821 g          460 t
ORIGIN      
        1 tctttgtcct gccgggcctg atcatgatcg gctggtggac cggctttccc tattggctct
       61 cggttgcggt gatcgcggtc ggcgggatcc tgggggtgat gtattcggtg ccgctgcgcc
      121 gcgccctggt gaccggatcg agcctgccct atcccgaagg cgtggcggcg gccgaggtgc
      181 tcaaggttgg tgctggcgtc ggcggggcag aggaaaaccg caagggtctg gccgcgatcg
      241 tgctgggatc ggtgctttcg gccggataca cgctgctcgc caagatgaag gtggtggccg
      301 aggaagcggc ggtcaccttc aagctagggg ccggggcgac cagcttttcg accagtttct
      361 cgctcgcgct gatcggggtc gggcacctgg tgggcatggc ggtggggatc gccatgctgg
      421 tcgggctggt gatcggctgg atcgtgctgt tgccgcaatt taccgccggt ggcctgccgg
      481 ccggggtgga attggcggat ttcgtttcga ccacgttccg cgaaaaggtg cgcttcgtgg
      541 gtgccggcac gatcggcgtg gccgcgatct ggaccctgct caagatgatc ggcccgatcg
      601 tcaagggcat tgccggggcg cttgccgcgg cgcgcgcgcg caaggcgggg gccggggcaa
      661 gcctcgacat taccgaacag gacctgccga tcgggatcgt cggtggcacc attgtggtgg
      721 ccatgctgcc gatagccatt ctgttggccg gctttgccgc gggcgggccg atcgaggcca
      781 tgctgggtac aacgcttggc ctgtcgatca tctatgtgtt gctggcgggc gtggtgatcg
      841 cttcggtctg cgggtatatg gccggcctga tcggtgcctc gaacagcccg atttcgggtg
      901 tgggcattct ggcaagtctt ggcatttcgc tgctgctggc gatgatcttc ggccagggcg
      961 gcgatgaagc caccaccaag gccctggtag ccttcgcgct gtttgtcacc gcgatcgtct
     1021 tcgggatcgc gacgatttcg aacgacaatc tgcaggatct gaagaccggg caactggttg
     1081 gcgccactcc ttggcgccag caggttgcgc tgatcctggg ggtgatcttc ggcgcattgg
     1141 tgatcccgcc ggtgctcgat ctgcttaaca ccactttcgg gttccagggg gctcccggcg
     1201 cgggcgaaga tgccctggcc gcaccacagg ccgcgctgat caccgcgatc gcccagggcg
     1261 tgcttggcgg caatctcgat tggcaactga tcggaaccgg cgccgcgatc ggcgggggcg
     1321 tggttctggt tgacgaactg ctgcgtcgca gcggcaaggg cagcctgccg ccgctgggcg
     1381 tgggcatggg catctacctg ccgatggcgc tgaccttgct gattccggtg ggggcgatca
     1441 tgggcttcgt ctatgacagg tgggcgcgcc gccagcagga tcacggcttt gccgaacgga
     1501 tgggcgtgct ggccgcaacc ggcctgatcg ttggcgaaag cctgtttggg gtggcctttg
     1561 ccggcatcgc cgcggccacc ggcagtccgg aagccctgtc gatcgttgaa gagaacggct
     1621 gggccgtgcc gatggcgctg ctggcgttcc cggcggcagt ggtctggctc tatgccgcga
     1681 ctcgcaaggc ggcctcgcgc gcgggctagg gctcgggcta aggcttacgt gttgttgacc
     1741 acccggcgca cgcagcgcag cgggccttcc ttgccatcgg attccagctt gcgaaggaaa
     1801 tttgcggaca ggcggcggaa ttgctgcccg gctttcgggc cgctggtcat gctcagcttg
     1861 tcgccggtga gcagataggt gccgcgtccg cccgtgcttt cataggtgct ggcgttcaga
     1921 acggtgaagc tttccgcatc ctggcgatgg ccggcagggc ccgtggcatc gcccggcagt
     1981 tcgcagatgt aggtgcccgg aaccagcgaa tcgatcattc cacccggcac ggcaagtgcc
     2041 ggaccgctgc caagaaccgt gataatggcc agtgcctgct tcatggcgcc ccctttgctg
     2101 cccgcgcctt agcagcaggt gaacgccaat gccaccttgc ccgatgcgcc cgggcgcgct
     2161 aagggcgcga cctttccgga cttccgggct ctttctcaac cttgaaggca ggcatcatgg
     2221 ccaagatcag cggcgtggac atccgtcccg gcaacattct cgaatacgaa ggcggcatct
     2281 ggaaggtcgc caagacccag cacacccag
//
LOCUS       DASUDV010000441         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_721916,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000441 DASUDV010000000
VERSION     DASUDV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721916"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFV30_09555"
     CDS             <1..444
                     /locus_tag="VFV30_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX4848378.1"
                     /translation="TRAGNFSRSADGQLVTAQGYPLQPAITIPEDASAITIAPDGTVS
                     VTTAGNAAPTEVGQLTVAAFANPAGLQALGDNFLAETAASGAAQIGPAGDGGRGAIRQ
                     GMLEASNVNVVEELVDMIECQRAYEINSKMISAVDEMLRNANQTL"
     gene            525..1250
                     /locus_tag="VFV30_09560"
     CDS             525..1250
                     /locus_tag="VFV30_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX4848379.1"
                     /translation="MARPNSRVGESNRPSPGPSHKWEGRLLIAALGALSLSTQACAGP
                     KPSAGFEAALPPPPPAPRSADGSIFNPGDGYAALYQGHRASRIGDPITIVLVERTATS
                     KSAGSKSEKSGSFGVTPPPTGPLSFIKPGSLNAGGDVSFKGDGSATQSSTLAGELSVT
                     IVEVRPGGTALVRGEKRLMLSQGQEWIQFSGIVRLADIGADNRIASTRVADARIEYAG
                     NGPVSRASREGWLSKFFNAISPF"
     gene            1262..2359
                     /locus_tag="VFV30_09565"
     CDS             1262..2359
                     /locus_tag="VFV30_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX4848380.1"
                     /translation="MLRILLIFAVLAGALAPLPVRAERVRDLGTFQGVRSNQLTGYGI
                     VVGLAGTGDDSLEYLTQGMHGVSGRVGVQLPPGVNPALKNAAAVIITADLPAFAKPGQ
                     RIDVTVSAIGKAKSLRGGALILAPLYGADGQIYAMAQGNLAVGGLGISGADGSKLTVN
                     VPTVGRIADGASVERTVATGFDSGASLRWNLYQSDFLTATRVRDAINARWPGTASIVD
                     GVTLSLRLPADPDARASQMAAIEMIDVTPAEAPARVVVNSRSGTVVINSAVRLYPAAI
                     SHGKLTVRIEENPQVIQPAPFSRGQTAVQPDSTLSAQEEGNRVVLFKPGASLAKLVDA
                     LNLLGVSPSDLVAILEALKQAGALKAEMVVI"
BASE COUNT          393 a          857 c          814 g          360 t
ORIGIN      
        1 acccgcgccg gcaatttcag ccgctcggcc gatggccagc tggtgaccgc gcagggctat
       61 ccgctgcagc cggcgatcac gatcccggaa gacgcctcgg cgatcacgat tgccccggac
      121 gggaccgtct cggtcaccac cgccggcaat gccgcgccga ccgaggtcgg ccagctgacc
      181 gtcgcggcct ttgccaatcc cgccgggctg caggcgctgg gcgacaactt cctggccgaa
      241 accgccgcca gcggcgcggc ccagatcggc ccggccggcg acggtgggcg cggggcgatc
      301 cgccagggga tgctggaagc gtccaacgtc aacgtggtcg aggaactggt cgacatgatc
      361 gaatgccagc gcgcctacga gatcaattcc aagatgatca gcgccgtcga cgagatgctg
      421 cgcaacgcca accagacgct gtgaggaccg ccatgaatcc ttatcccaag acggtatccg
      481 gtaagctccc ctcccgcttg cgggaggggt tgggggaggg cctgttggct cgccccaatt
      541 cccgcgttgg agagagtaac aggccctccc ccggcccctc ccataaatgg gaggggagac
      601 ttctgatcgc ggcgctaggc gcgctcagcc tttcgacgca ggcctgcgca ggtcccaagc
      661 cctctgccgg tttcgaagcc gccctgcccc cgcccccgcc cgcgccgcgc agcgccgatg
      721 gatcgatctt caatcccggc gatggctatg ccgcgcttta ccagggccac cgcgccagcc
      781 ggatcggcga tccgatcacg atcgtgctgg tggaacgcac cgccacatcg aaatcggccg
      841 gatcgaaaag cgagaagtcc ggcagcttcg gggtcacccc gccgccgacc ggcccgctca
      901 gcttcatcaa gcccggcagc cttaatgccg gcggcgacgt gtcgttcaag ggagacggaa
      961 gcgccaccca gtcgagcacg ctcgccggag agctttcggt cactattgtc gaggtgcggc
     1021 cgggcgggac cgcgctggtt cgcggggaaa agcgcctgat gctgagccag ggacaggaat
     1081 ggatccagtt ttccgggatc gtgcggctgg ccgatattgg cgccgacaac cggatcgcct
     1141 ccacccgggt ggccgacgcg cggatcgaat acgccggcaa cggcccggtc agccgcgcca
     1201 gccgcgaagg ctggctgtcc aagttcttca acgccatcag cccgttctga gggaggccgt
     1261 catgctgcgc atcctgctga tcttcgccgt gctggccggg gccctcgccc cgctgcccgt
     1321 gcgggccgaa cgggtccgcg atctgggcac ctttcagggc gtccgttcca accagctgac
     1381 cggctatggc atcgtcgttg gcctggccgg caccggggac gacagcctgg agtacctgac
     1441 ccaggggatg cacggcgtct ccggccgggt cggagtgcaa ttgccgcccg gggtcaaccc
     1501 ggcgctgaaa aacgccgcag cggtgatcat cactgccgac ctgccagcct tcgccaagcc
     1561 cggccagcgg atcgacgtga ccgtctcggc gatcggcaag gccaagtcgc tgcgcggcgg
     1621 ggcgctgatc ctggctccgc tctatggcgc cgacgggcag atctatgcca tggcccaggg
     1681 caacctggcg gtcggcggcc tcggcatttc cggggccgat ggatcgaagc tgacggtcaa
     1741 tgttcccacg gtcggccgga ttgccgacgg ggccagcgtg gagcgcacgg tcgcaaccgg
     1801 gttcgattcc ggggcgagcc tgcgctggaa cctctaccag tccgacttcc tcaccgcgac
     1861 gcgggtccgc gatgcgatca acgcgcgctg gccgggcacc gcctcgattg tcgacggggt
     1921 caccctgtcg ctgcggctgc cggccgatcc cgacgcccgc gccagccaga tggccgcgat
     1981 cgagatgatc gacgtgaccc cggccgaggc cccggcccgg gtggtggtca attcgcgcag
     2041 cggcacggtg gtgatcaatt cggccgtgcg gctgtacccc gcggcgatca gccacggcaa
     2101 actgaccgtg cggatcgagg agaaccccca ggtgatccag cccgccccgt tcagccgcgg
     2161 ccagaccgcg gtgcagccgg acagcacgtt gtcggcccag gaggagggca accgcgtggt
     2221 gctgttcaag cccggtgctt cgctggccaa gctggtcgat gcactcaacc tgctcggcgt
     2281 cagcccgtcc gaccttgtcg cgatcctcga agcgctcaag caggccggcg cactcaaggc
     2341 cgaaatggtg gtgatatgac cgcgatcggc gcgcccctgc ttgccaccgg cagcctgccg
     2401 gccagccacg gcagcgaccg cgaa
//
LOCUS       DASUDV010000442         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2091434,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000442 DASUDV010000000
VERSION     DASUDV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091434"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFV30_09570"
     CDS             complement(<1..311)
                     /locus_tag="VFV30_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4848381.1"
                     /translation="MRTAIAGAARATGIDFDYLLGQARIESGLNPQARAGTSSAAGLY
                     QFTQGTWLETLDRHGAAHGYSWASAAIENGRVRDPALRRDILAMRFDPTAAALMAGEL
                     AG"
     gene            complement(440..799)
                     /locus_tag="VFV30_09575"
     CDS             complement(440..799)
                     /locus_tag="VFV30_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEX4848382.1"
                     /translation="MSGSEHMRENLRQMIAVLQAERQALAGLDLDAIMGCAADKVTLC
                     GTLDDCGPFADGFAVDEECRGLLDAARRMNEVNRQVRNLIAANVSARLDALTGSPALY
                     RAGTTASAPYSLAGARG"
     gene            complement(796..1089)
                     /gene="flgM"
                     /locus_tag="VFV30_09580"
     CDS             complement(796..1089)
                     /gene="flgM"
                     /locus_tag="VFV30_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEX4848383.1"
                     /translation="MPSIEFGPKGPHAARAVPAVDARKVPQAPVGSDRAAEVANPNSA
                     VVRSRELDPGQPPVDADRVQQIRKAIEEGTYPIVPAKVADAMIAAGLLLRSGK"
     gene            complement(1173..1679)
                     /locus_tag="VFV30_09585"
     CDS             complement(1173..1679)
                     /locus_tag="VFV30_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEX4848384.1"
                     /translation="MTRIPLSAAALALALGAPALAQSAVADLAAIDRAVAQFTGMPQG
                     APGGAALPVDRRLRLTPCRAPLALGWYGARRDTVEVVCPMPGGWKLYVPLVGPADGGV
                     AAAPLIARGDAVTITVSGDGFAVSQPGEALESGAEGAWIKVRGINPKAPVLRARVLRP
                     GVVGIDLP"
     gene            complement(1676..2092)
                     /locus_tag="VFV30_09590"
     CDS             complement(1676..2092)
                     /locus_tag="VFV30_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848385.1"
                     /translation="MIARAGSGWQTVLADLSLILFMVTAAAVSQQPAAAPARPAPAAL
                     PALGEPVAQWRAGPDAPALSAWLRSSGGDPRLRLTILAGPDSAAAALALAAGAGRPAR
                     VLIEPGASGIAATLTFDQPPALARGLQDGPAKETLR"
     gene            complement(2089..2781)
                     /locus_tag="VFV30_09595"
     CDS             complement(2089..2781)
                     /locus_tag="VFV30_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4848386.1"
                     /translation="MDMMQLVDGPSAAIVGGGTLLATLLRAGFGDCRVTLIKLGQLGR
                     SPFDAQAARAELGAQIQEIRKDGLLRAVPHRYGDSEFDEATEALIGNRSVAALVKAHE
                     KHKARRLAEAERAVRTLAQAAELAPVFGLAGTLVSLSQLPAEGLAQGALGGAISMAVL
                     TTLYGLLAANLLLAPLSRMVERAAQREERQRQELIDWLAWQVADACPTAARPERPLRP
                     ERPAAAPLAEAS"
     gene            2968..3312
                     /gene="flgB"
                     /locus_tag="VFV30_09600"
     CDS             2968..3312
                     /gene="flgB"
                     /locus_tag="VFV30_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX4848387.1"
                     /translation="MGSLFGIHGDALQLRSQRLGLIASNIANAGTPGYKARDIDFAAA
                     LSASQRGLGPDAAGAAATRYRVPVMPAMDGNTVEMATEQVAFAETAVGYQATLTFIRS
                     RVENLTRALKGE"
     gene            3315..3716
                     /gene="flgC"
                     /locus_tag="VFV30_09605"
     CDS             3315..3716
                     /gene="flgC"
                     /locus_tag="VFV30_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX4848388.1"
                     /translation="MGGLSLFGLAQRGMSAQLVRMNAAASNLANAGSVTGTEAEAYRP
                     IRPVFAEQYDAASGLSTVGVSGVIRTTAAPIKQYDPDHPLADAEGNVWTAPVDENAEM
                     VEMLDASRQYQNLVEALSTAKQLMLETMRMK"
     gene            3713..3982
                     /locus_tag="VFV30_09610"
     CDS             3713..3982
                     /locus_tag="VFV30_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX4848389.1"
                     /translation="MTVNAITSAGQAAAAKPASQTLGQSDFIRLMTTQMKMQDPTDPV
                     DNKEMIAQMAQFSSLSGIENINTTLKAISAQLDQALSARSSTPAV"
     gene            3993..4805
                     /locus_tag="VFV30_09615"
     CDS             3993..4805
                     /locus_tag="VFV30_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HEX4848390.1"
                     /translation="MSFYTSLNGLKNAQTELSVIANNLANAETTGFKKSRINFADIVS
                     SAASTNPQLVKGIGSTVQSIDQNFSLGPIQQTGAALDLAIDGEGFFTKVSPITGKTFY
                     TRNGNFDIDGAGNITDTVGNRLQVLPVDAAGNVTSTTPQNALAPLTNGAGSEFVGITV
                     KTDGSIMASYADGSVSSIGKVALAAFVAPTGLLQVGNQDWQATGISGAATYNQPGAGR
                     FGNILSGSLEQSNVDIAEEMVGLITAQRYFQANAKAIDTATQLSQTIVNLRT"
     gene            4815..5555
                     /gene="flgF"
                     /locus_tag="VFV30_09620"
     CDS             4815..5555
                     /gene="flgF"
                     /locus_tag="VFV30_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HEX4848391.1"
                     /translation="MDRLIWTAVTGMNASMARQRMIASNMANSQTVGFRAEVMAQRPM
                     TLLGPTLGVRAMSDASVQGARMDEGALVNTGRPLDIALQGQALMAVQAPDGSEAYTRR
                     GDLAVSPSGLLTNGEGFPVIGESGPISVPPGSVVTIGTDGAVLAANPETPDAPPQQID
                     RIKLASWQGSRIDKGLDGLFRVVGGGALPSDAEARVIPATLEQSNVRPAEVLVEMVEA
                     QRLFEMRTQLIATAKELDEGGASLMRIS"
     gene            5596..>5714
                     /locus_tag="VFV30_09625"
     CDS             5596..>5714
                     /locus_tag="VFV30_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEX4848392.1"
                     /translation="MPTSALHVARTGLEAQDARMRVIANNLANIGTTGFKRDRA"
BASE COUNT          920 a         2079 c         1927 g          788 t
ORIGIN      
        1 ccggccagtt ccccggccat cagcgcggcg gcggtgggat cgaaccgcat cgccaggata
       61 tcgcgccgca gcgccggatc gcggactcgg ccgttctcga tcgcggcgct ggcccagcta
      121 tagccgtggg ccgcgccgtg gcggtccagc gtttccagcc aggtgccctg ggtaaactga
      181 tagagccccg ccgcgctgga ggtgccggcg cgggcctggg ggttgaggcc ggattcgatc
      241 cgggcctggc ccagcaggta atcgaaatcg atacccgtgg cgcgggccgc cccggcaatc
      301 gcggtgcgga tctcgggccg ggccggggcc ggtccgcggc tgcgggcggg cgccggtgcg
      361 gcgggggttg cggggtcgct caagccttgc tcccgatgca aaaggacatc gggaggggtt
      421 cagcaagccc cgtgccaaat cagccgcggg cacctgccag gctgtaggga gcgctggccg
      481 tggtcccggc ccggtagagc gccggactgc cggtcagcgc atcgagccgg gccgagacat
      541 tggccgcgat caggttgcga acctggcggt ttacctcgtt catccgccgc gccgcgtcga
      601 gcaggccgcg gcattcctcg tccacggcaa agccatcggc aaaggggccg caatcgtcca
      661 gcgtgccgca cagcgtgacc ttgtcggccg cgcagcccat gattgcgtcg aggtcgagcc
      721 cggcaagcgc ctgccgctcg gcctgcagga cggcaatcat ctgccgcagg ttctcgcgca
      781 tgtgttccga accgctcact tgccgctcct cagcaacagg cccgccgcga tcatcgcatc
      841 ggccaccttg gccggcacga tcggataggt gccctcttcg atcgccttgc ggatctgctg
      901 gacccgatcg gcatcgaccg ggggctggcc cggatccagt tcgcggctgc gaaccacggc
      961 ggagttgggg ttggccactt cggcggcccg gtcgctgccg accggggcct gcggcacttt
     1021 gcgcgcgtcc accgccggga cggcgcgcgc cgcgtggggt cctttcgggc cgaattcgat
     1081 ggaaggcatg gtgccgctcc ttggttgctt atcgaacccc agaacggcag cgccgccgcc
     1141 ggtttaaggg ccggcggcaa gggaatgccg gatcacggca agtcgatgcc gaccaccccg
     1201 gggcgcagca cccgggcgcg cagcaccggg gccttgggat tgatgccccg gaccttgatc
     1261 caggcccctt cggccccgga ttccagcgcc tcgcccggct gcgacacggc aaagccatcg
     1321 ccgctgacgg tgatcgtcac cgcatcgccg cgggcaatca gcggcgcggc ggccacgccc
     1381 ccatcggccg ggccgaccag cgggacatag agcttccagc cgcccggcat cgggcagacc
     1441 acttccaccg tatcgcgccg cgcaccgtac cagcccagcg ccagcggcgc tcggcagggg
     1501 gtcaaccgca gccgccggtc gaccggcagc gccgccccgc ccggcgcgcc ttgcggcatg
     1561 ccggtaaact gggcgaccgc gcggtcgatt gcggccagat cggccacggc ggactgggcc
     1621 agggccggcg cgccaagcgc cagagccagg gcggcggcag aaagcggaat acgggtcatc
     1681 gcagggtctc ctttgccgga ccatcctgca aaccccgtgc cagcgcgggt ggctggtcaa
     1741 aggtgagggt ggcggcgatc ccgcttgcac ccggttcgat cagcacccgg gccggccgcc
     1801 ctgcccccgc cgccagcgcc agcgccgcag cggcgctgtc cggcccggcc aggatcgtga
     1861 gccgcagccg cggatcgcct ccgctggagc gcagccaggc gctgagcgcg ggcgcatcgg
     1921 gcccggcgcg ccactgcgcc accggctcgc ccaaagcggg aagcgcggcc ggtgccggcc
     1981 gggccggcgc cgcagcgggc tgctggctga ccgccgcggc cgtgaccatg aacaggatca
     2041 gcgaaaggtc ggccagaacg gtctgccagc cgctgcccgc gcgggcgatc acgaggcttc
     2101 cgccagcggc gcggcggcgg gccgctcggg ccgcagcggg cgctcaggcc gcgcggccgt
     2161 ggggcaggcg tcggcgactt gccaggccag ccagtcaatc agttcctggc gctggcgttc
     2221 ctccctctgg gccgcccgct cgaccatgcg cgacagcggg gcgagcagca ggttggcggc
     2281 cagcaggcca tagagcgtgg tcaggaccgc catgctgatc gccccgccca gcgcgccctg
     2341 cgccagccct tcggccggca gctggctgag cgagacgagc gtaccggcca ggccgaacac
     2401 cggggccagc tccgccgcct gggccagggt ccgcactgcg cgctccgcct cggccaggcg
     2461 gcgggccttg tgcttctcat gcgccttgac cagcgccgcg accgaacggt tgccgatcag
     2521 cgcctcggtc gcctcgtcga actcgctgtc gccatagcgg tgcggcacgg cgcgcagcag
     2581 gccgtccttg cggatctcct ggatctgcgc gcccagctcg gcccgggcgg cctgggcatc
     2641 gaacgggctg cggcccagct ggcccagctt gatcagcgtc acccggcaat cgccgaaccc
     2701 ggcgcgcagc agcgtggcca aaagggttcc gccgccaaca atcgcggccg atgggccgtc
     2761 caccagttgc atcatgtcca tcgtatcggg gctccttcac cggcgtagga cggcaggcgg
     2821 caagcctcct tcagggcacg gcggaacttt gccgccaacc ggcaagccct tgccgcccgg
     2881 ccggccccgc agccgcgcat ttccgcgctt ttccgatctg gcacgggcct tgcgaacact
     2941 tccgcgacac ttgcgatgga ggtgccgatg ggcagcctgt tcggaattca cggagacgcc
     3001 ctgcagctgc gttcgcagcg gctggggctg attgccagca acatcgccaa cgccggcacc
     3061 cccggctaca aggcccgcga tatcgatttc gcggcggcgc tcagcgccag ccagaggggg
     3121 cttggccccg acgccgccgg cgcggccgcc acccgctacc gcgtgccggt gatgccggcg
     3181 atggacggca acacggtgga aatggccacc gagcaggtcg cctttgccga aaccgccgtc
     3241 ggctatcagg ccacgctgac cttcatccgc agccgggtcg aaaacctgac ccgcgcgctg
     3301 aagggcgaat gacgatgggc ggcctctcgc tcttcggcct cgcccagcgc ggcatgtcgg
     3361 cccagctggt gcggatgaac gccgcggcct ccaacctcgc caacgccggt tcggtgaccg
     3421 gcaccgaggc cgaggcctat cgcccgatcc gcccggtctt tgccgagcag tacgatgccg
     3481 ccagcggcct ttccacggtc ggcgtcagcg gcgtgatccg caccaccgcg gcgccgatca
     3541 agcaatacga tcccgatcat cccctggccg atgccgaggg aaatgtctgg accgccccgg
     3601 tcgatgaaaa cgccgagatg gtcgagatgc tcgatgcctc ccgccagtac cagaacctgg
     3661 tcgaggcgct gtccaccgcc aagcagctga tgctcgaaac catgaggatg aaatgaccgt
     3721 caacgcgatc acttcggcgg gtcaggccgc cgccgccaaa ccggcttcgc agaccctggg
     3781 ccagtcggac ttcatccgcc tgatgaccac ccagatgaag atgcaggacc cgaccgaccc
     3841 ggtcgataac aaggagatga tcgcccagat ggcgcagttt tcatccctgt ccgggatcga
     3901 gaacatcaac accacgctga aggcgatttc cgcccagctc gaccaggctc tgtccgcccg
     3961 gtcctccacc cccgcagtct aggagccagc ccatgtcgtt ctacacctcg ctcaacggcc
     4021 tcaagaacgc gcagaccgaa ctgtcggtca tcgccaacaa cctggccaac gccgaaacca
     4081 ccggcttcaa gaaaagccgg atcaacttcg cggacatcgt gtccagcgcg gcttccacca
     4141 acccgcagct ggtcaagggg atcggttcaa ccgtccagtc gatcgaccag aacttttcgc
     4201 tcgggccgat ccagcagacc ggcgcggcgc tggacctggc gatcgacggc gaaggctttt
     4261 tcaccaaggt cagcccgatc accggcaaga ccttttacac ccgcaacggc aacttcgaca
     4321 tcgacggggc cggcaacatc accgacacgg tcggcaaccg cctgcaggtc ctgccggtcg
     4381 atgccgccgg caatgtcacc tccaccaccc cgcagaatgc gctggccccg ctgaccaacg
     4441 gcgcgggcag cgaattcgtg gggatcacgg tcaagaccga cgggtcgatc atggcctcct
     4501 atgccgacgg ttcggtgagc tcgatcggca aggtcgcgct ggccgccttc gtcgcgccga
     4561 ccgggctgct gcaggtcggc aaccaggact ggcaggccac cgggatttcc ggggcggcga
     4621 cctacaacca gcccggcgcg ggccggttcg gcaacatcct gtcgggcagc ctggagcagt
     4681 cgaacgtcga cattgccgag gaaatggtcg ggctgattac cgcccagcgc tatttccagg
     4741 ccaacgccaa ggcgatcgat accgcgaccc agctgtcgca gaccatcgtc aacctgcgga
     4801 cctgaggtta gggcatggac cgcctgatct ggaccgccgt gaccggcatg aacgcgtcga
     4861 tggcgcggca gcggatgatc gccagcaaca tggccaattc gcagaccgtg ggctttcgcg
     4921 ccgaagtgat ggcgcagcgg ccgatgaccc tgctgggtcc gaccctgggc gtgcgcgcga
     4981 tgagcgacgc ctccgtccag ggcgcgcgga tggacgaggg cgcgctggtc aacaccggcc
     5041 gcccgctcga catcgccctg cagggccagg ccctgatggc ggtccaggcc cccgatggca
     5101 gcgaagccta tacccgccgg ggcgacctgg cggtgtcccc cagcggcctt ctgacgaacg
     5161 gcgaaggctt tccggtgatc ggcgagagcg ggccgatctc ggtcccgccc ggcagcgtgg
     5221 tgacgatcgg gaccgacggc gccgtgctgg ccgccaaccc ggaaacgccc gatgccccgc
     5281 cccagcagat cgaccggatc aagcttgcca gctggcaggg cagccggatc gacaaggggc
     5341 tggacgggct gttccgggtg gtcggcggcg gggccctgcc cagcgatgcc gaggcccggg
     5401 tgatcccggc cacgctggaa caatcgaacg tccgcccggc cgaagtgctg gtcgaaatgg
     5461 tcgaggccca gcgcctgttc gaaatgcgca cccagctgat cgcgaccgcc aaggagctcg
     5521 acgagggcgg cgccagcctg atgcggatct cctgatcccg ccccgtttca gaccaacgat
     5581 ccaaaggaat ttccgatgcc cacctccgcc ctgcacgtcg cccgcaccgg cctggaagcc
     5641 caggacgcgc ggatgcgcgt catcgccaac aacttggcca acatcggcac caccgggttc
     5701 aagcgcgacc gcgc
//
LOCUS       DASUDV010000443         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_147453,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000443 DASUDV010000000
VERSION     DASUDV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147453"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFV30_09630"
     CDS             complement(<1..234)
                     /locus_tag="VFV30_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX4848393.1"
                     /translation="MRLVAATALVLSLAACGQRADLKPVAGKTLPVAPYGREDRPSAE
                     ALTQTTTQAVPERSVELRKRSEERTLDPFDLPPS"
     gene            complement(239..1612)
                     /gene="argH"
                     /locus_tag="VFV30_09635"
     CDS             complement(239..1612)
                     /gene="argH"
                     /locus_tag="VFV30_09635"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX4848394.1"
                     /translation="MWGGRFAEGPSAIMREINASIPFDKALWRQDIAASKAHVAMLGA
                     QGIVSAEDAATIAAGLDSIAAEYEANGVPENWDLEDIHMTTESRLAELIGPAAGRLHT
                     ARSRNDQVATDFKLWVREACVQADAALGALQVALVTRAGEHAASIMPGFTHLQTAQPV
                     TLGHHLMAYYEMIERDRSRFVDAHRRASQCPLGSTALAGTGFPIDREATASALGFAMP
                     TRNSLDSVSDRDFALDYLMAASSCALHLSRLAEEFIIWASQPFGFVRLPDALSTGSSI
                     MPQKKNPDAAELVRGHSGRIAGCLTSLMISMKGLPLAYSKDMQDDKPPVFEAAGLLML
                     CLAAMTGMVEASEFRTERMRGAAELGYATATDLADWLVRQANVPFREAHHITGAAVKL
                     AESRGLALDQLPLADLQAIDPRISAEVFAALSVESSVAARASYGGTAPEQVRARVAEA
                     RAALGLE"
     gene            1779..2180
                     /locus_tag="VFV30_09640"
     CDS             1779..2180
                     /locus_tag="VFV30_09640"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4848395.1"
                     /translation="MPDFTFADTAGKELQLKTLRGKPVLLNLWATWCAPCVAELPALD
                     RLVQSGAIRVVTISQDMGQPEKVAAFLKQRGVTRLEPWLDPKSDLAFHYGAAPLPISV
                     LYGSDGREVWRMSGPLEWDEPEVRKLVAEAK"
BASE COUNT          418 a          781 c          829 g          422 t
ORIGIN      
        1 ggaaggaggc aggtcgaagg gatcgagagt gcgttcttcc gaacgttttc gaagctcgac
       61 gctgcgctct ggcacagcct gggtggtggt ctgggtcagt gcttcggcgc tgggccggtc
      121 ctcgcggcca tagggagcaa ccggaagcgt cttgcccgcc acgggtttga ggtcggcacg
      181 ctggccgcag gcggcaagcg agaggaccag ggcggtggcg gcaacaaggc gcatcgttct
      241 actccaggcc gagcgcagcg cgcgcttcgg ccacgcgggc ccttacctgt tccggcgcgg
      301 ttccgccata gctggcgcgc gccgcgaccg aggattcgac cgaaagcgcc gcaaatacct
      361 ctgcgctgat ccgcggatcg atcgcctgga gatcggcgag cggcagctga tcgagcgcca
      421 gcccccggct ttcggccagc ttcaccgccg cgccggtgat atggtgggct tcgcggaagg
      481 gcacattggc ctggcgcacc agccaatcgg ccagatcggt tgccgtggca tagcccagtt
      541 cggcagcacc ccgcatccgc tcggtgcgga attcgctggc ctcgaccatc ccggtcatcg
      601 ccgccaggca cagcatcagc agccccgccg cctcgaacac cggcggcttg tcgtcctgca
      661 tgtccttcga ataggccagc ggcagcccct tcatcgagat catcaggctg gtcaggcagc
      721 ccgcgatccg cccggaatgg ccgcgcacca gttcggccgc gtcggggttc ttcttctgcg
      781 gcatgatcga gctgccggtg gacagcgcat cgggcaggcg gacaaagccg aacggctggc
      841 tggcccagat gatgaattcc tcggccagcc gcgagaggtg cagcgcgcaa ctgctggccg
      901 ccatcaggta atcgagcgcg aaatcgcggt ccgacaccga atcgaggctg ttgcgggtgg
      961 gcatggcaaa gcccagcgcg cttgccgtgg cctcgcggtc gatcggaaat ccggttccgg
     1021 ccagcgcggt gctgcccagc gggcactggc tggcgcggcg gtgcgcgtca acgaagcgcg
     1081 aacgatcgcg ctcgatcatt tcgtaatagg ccatcaggtg gtgaccaagg gtaaccggct
     1141 gcgcggtttg caggtgagtg aagccgggca tgatcgaggc ggcatgctcg cccgcgcggg
     1201 tcaccagcgc cacctgcagc gcgcccagcg cggcatccgc ctggacgcag gcttcgcgta
     1261 cccacaactt gaaatcggtt gctacctgat cgttgcggct gcgcgcggtg tggaggcgcc
     1321 cggcggcggg gccgatcagt tcggccagcc ggctttcggt agtcatgtgg atgtcttcaa
     1381 ggtcccagtt ttcgggaacc ccgttggcct catactcggc agcgatactg tcgagcccgg
     1441 cggcaatcgt cgccgcatcc tcggccgaaa cgatcccttg cgcgcccagc atcgcgacat
     1501 gggccttgct tgcggcgatg tcctgacgcc acaatgcctt gtcgaagggg atcgaggcat
     1561 ttatctcgcg catgatcgcg gacggccctt cggcaaagcg tccgccccac atctggttgg
     1621 agccgcccgt gttctcgtcc cgcttgctca atgccgtgat ccttggctgt gccctgcttg
     1681 tcgcggggtg cgataggcaa agcggcgagc ccgcgcaacc gcaagcaaca tcgcccgccc
     1741 agcccggaac aatcgatcgc agcaacaagg gcagcgaaat gcccgatttc acctttgccg
     1801 ataccgcagg caaggagctt cagctcaaga ccctcagggg caagccggta ctgctgaacc
     1861 tgtgggccac ctggtgcgcg ccgtgcgtgg ccgaactgcc cgcgctcgat cggctggtcc
     1921 agtcgggggc gatccgggtg gtgacgattt cgcaggacat gggccagccc gaaaaggttg
     1981 ccgccttcct caagcagcgc ggcgtgaccc ggcttgaacc ctggctcgat cccaagagcg
     2041 accttgcttt ccactatggc gcagcaccgc tgccgatttc ggtgctctat ggctcggacg
     2101 ggcgtgaggt gtggcgcatg tcggggccgc tcgaatggga cgagcccgag gtgcgcaagc
     2161 tggttgccga agcgaagtag accctgatga ggttacatgg acccgccctg acggagccga
     2221 ttttggtttg tggggaggcg tgaggaggga gcaatgctgt cgcattgtga ccgacgaaca
     2281 acgctgttca cggactttcc ctgcccaatc agggtggatc aaggtaacgt catcagggtc
     2341 taagctcagg cgaaatgcag gcggcggtac ttgccgcgga aatagagcag cggatcgcgg
     2401 cgtggctcga actgggcgcg cagcacttcg cccaccagga tgaagtgatc
//
LOCUS       DASUDV010000444         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2179953,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000444 DASUDV010000000
VERSION     DASUDV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179953"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFV30_09645"
     CDS             complement(<1..525)
                     /locus_tag="VFV30_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX4848396.1"
                     /translation="MAGTDTVDKTVEIVIIAMVAAFLGLRLYSVLGRRAEHEEHPLPP
                     RFEPKEPRATDLSGRMPAGDLQGGAAAPRPLVPGVTPAIERGVREIAAADRNFDMLAF
                     LEGAKGAYRMVLEAFWRGDRDALARLCDSDVQAAFNDAIDARLAAGEVLDNKLVRIED
                     AVIVAAEYRAPFARI"
     gene            753..1277
                     /gene="secB"
                     /locus_tag="VFV30_09650"
     CDS             753..1277
                     /gene="secB"
                     /locus_tag="VFV30_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041550025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX4848397.1"
                     /translation="MSDDGNIITDLNLDAPQPNGADTLPTAGIISQYVKDLSVENPNA
                     PESLNWQDAPQIDVQFNIGARELNAEISEVELKISITSKCEAGTVFIIDLSYGGLIGM
                     RNLEDQHKHAFTYAEAPRILFPFARRVISDAVRDAGFPPLLLEPIDFNGIYMQQLAQA
                     QGQGEAAGAPAGEA"
     gene            1310..2887
                     /gene="murJ"
                     /locus_tag="VFV30_09655"
     CDS             1310..2887
                     /gene="murJ"
                     /locus_tag="VFV30_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX4848398.1"
                     /translation="MNLLKSTGIIGGMTLISRIFGFVRDMMLSRLLGGSAMGDAWQIA
                     FQLPNIFRRLFAEGAFSAAFVPLFNRHVSQDGERDHAGAERFANETLSVFVPVLLVFS
                     AVMMALMPWAIWLIDDFGQGGRTNDTSIALARIAFPYLGLISVMTLFGAILNSISRFA
                     AVAFAPVLLNLCMIGAMLSGIYLGHAGDPARIALFLAWSVTLAGVLQMAWLYWHARRA
                     GFRFQLRRPRLTKGVRELGVLILPAVFGAGIYQISRFIDLFFLGRLEGGSFVFLALAD
                     RLNQLPLGIIGIALGTAILPALSRFIAVDDAGGAQRVQSNAIELGLLLTVPAAVGLYF
                     AAHPLVSAFYSGGKFGADDVNATAGVVAMLVIGLPAYVMVKVLTPGFFARRDTRTPVY
                     TAGISMVVNIGFNLLLIPIFGVAGLALAGSIAAWTNCALLYGVLKRRGHFAIEPDLLT
                     RIGRIALSAVAMGAAIHFLAPLGAAHYGAGALARVGSIVALVGVGAFVYFALAWLTGA
                     IDRNKIAMLTRRQAKSN"
     gene            2899..>3844
                     /gene="trpS"
                     /locus_tag="VFV30_09660"
     CDS             2899..>3844
                     /gene="trpS"
                     /locus_tag="VFV30_09660"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX4848399.1"
                     /translation="MRIVSGIQPTGNLHLGNYLGAIRNWVKMQDDVAAGGGQSLYFLA
                     DLHAISMPHSPAELRENTLEMTAALVACGIDPDKSVLFNQAQVPAHAELQWLLSGTGR
                     MGWLNRMTQWKDKAGKNREGQSVALFTYPVLQAADVLLYQATHVPVGEDQKQHLELAR
                     DIAQKFNNDFASEDAPVFTLPDPIIPPEAARIMSLRDGSAKMSKSDPSDMSRINLTDD
                     ADTIMQKVKKAKTDPEPLPGDKGGLDGRPEAANLVGIYAAMAGESQDAVLARFGGEGF
                     GKFKPALGELLVESLAPINARFLDLRQDREALDAILAKG"
BASE COUNT          620 a         1321 c         1243 g          660 t
ORIGIN      
        1 gatccgcgcg aacggcgcgc ggtattcggc ggcgacgatc accgcgtcct cgatccgcac
       61 cagcttgttg tcgagcactt cgccggccgc gaggcgggcg tcgatcgcat cgttgaaggc
      121 ggcctggaca tcgctgtcgc acagccgggc cagggcatcg cggtccccgc gccaaaaggc
      181 ttcgaggacc atgcggtagg cgcccttggc cccttcaagg aaggccagca tatcgaaatt
      241 acggtccgcc gcggcgattt cgcgcacgcc gcgttcgatc gccggggtca ctccgggaac
      301 cagcggacgc ggcgcggccg ccccgccctg cagatcaccg gcgggcatcc gcccgctgag
      361 gtcggtggcg cggggctcct tgggttcgaa ccgcggcggc agcgggtgct cctcatgctc
      421 ggcacggcgg cccagcacgg aatagagccg caggcccagg aaggccgcaa ccatcgcgat
      481 gatgacgatc tctacagtct tgtccacggt atcagtccca gccacgccgc gcggccactg
      541 ccacgccctg tagctgtaaa taggtacgca tgacaaatga acaacgcgtg gacggcactt
      601 tccgtgcgat ttttgggacc atcggtgccc gaaggccaca gcgcgccaac agcaccgcta
      661 catcgcctat aatcgttgcc gccgccgcgc ggcggtgcta ggcgcgccgc caaccgtatc
      721 aaccccttcc ttgagcgaaa gccagtacga ccatgtccga cgacggcaac atcatcaccg
      781 acctcaacct cgatgcgccc cagccgaacg gcgccgatac cctgcccacg gccgggatca
      841 tctcgcagta cgtcaaggac ctgtcggtcg agaacccgaa cgcgcccgaa tcgctcaact
      901 ggcaggacgc cccgcagatc gacgtgcagt tcaacatcgg cgcgcgcgag ctcaacgccg
      961 agatcagcga agtcgaactg aagatctcga tcacctcgaa gtgcgaggcc ggcacggtct
     1021 tcatcatcga cctgagctat ggcggcctga tcggcatgcg caacctggaa gaccagcaca
     1081 agcacgcctt cacctatgcc gaagcgccgc gcatcctgtt ccccttcgcg cgccgcgtga
     1141 tttcggacgc cgtgcgcgat gccggcttcc cgccgctgct gctggaaccg atcgatttca
     1201 acgggatcta catgcagcag ctcgcccagg cgcagggcca gggcgaagcc gccggggctc
     1261 cggccggcga agcctgatcc gcacaccggc tctggctggg tagcgtcgca tgaacctgct
     1321 gaagtccacc ggcatcatcg gcgggatgac gctgatcagc cggatcttcg gcttcgtgcg
     1381 cgacatgatg ctctcccgcc tgctgggcgg gagcgcgatg ggcgatgcct ggcaaatcgc
     1441 cttccagctg cccaacatct ttcgccggct cttcgccgaa ggggccttct ccgccgcctt
     1501 cgtgccgctg ttcaaccgcc acgtctcgca ggacggtgag cgcgatcacg ccggggccga
     1561 gcggttcgcc aacgagacgc tgtccgtctt cgtgcccgtc ctgctggtct tttccgccgt
     1621 catgatggcg ctgatgccct gggcgatctg gctgatcgac gatttcggac agggcggccg
     1681 gaccaacgat acctcgatcg cgctggcccg gatcgccttc ccctatctgg ggctgatcag
     1741 cgtgatgacg ctgttcgggg cgatcctcaa ttcgatcagc cgttttgccg ccgtcgcctt
     1801 tgccccggtc ctgctgaacc tgtgcatgat cggggcgatg ctgtccggca tttacctggg
     1861 gcatgccggc gatccggccc ggatcgccct gttcctggcc tggtcggtga cgctggccgg
     1921 ggtcctgcag atggcgtggc tttattggca cgcccgccgg gcgggtttcc gctttcaatt
     1981 gcgccgtccg cgcctgacca agggcgtgcg cgaactgggc gtgctgatcc tgccggccgt
     2041 gttcggcgcc gggatctacc agatcagccg cttcatcgac ctgttctttc tgggccgcct
     2101 ggaaggggga tcgttcgtgt tcctggccct ggccgaccgg ctcaaccagt tgccgctggg
     2161 gatcatcggc atcgcgctgg gcaccgcgat cctgccggcc ctgtcgcgct tcatcgcggt
     2221 ggatgacgcc ggcggcgcgc agcgggtgca gtccaacgcg atcgagctgg gcctgctgct
     2281 gaccgtgccg gccgcggtcg ggctctattt cgccgcgcac ccgctggtca gcgcgttcta
     2341 ttcgggcggc aagttcggcg cggacgacgt caacgccacg gcaggcgttg tggccatgct
     2401 ggtgatcgga ctgccggcct atgtcatggt caaggtcctg acccccgggt tcttcgcccg
     2461 ccgcgacacc cgcacgccgg tctataccgc cgggatctcg atggtggtca acatcggctt
     2521 caacctgctg ctgatcccga tcttcggcgt ggccgggctg gccctggccg gatcgatcgc
     2581 ggcctggacc aactgcgcgc tgctgtatgg cgtgctcaag cggcgcgggc atttcgcgat
     2641 cgaaccggat ctgctgaccc ggatcgggcg gattgcgctg tcggccgtgg ccatgggggc
     2701 ggcgatccat ttccttgccc cgctgggcgc ggcccattac ggcgccgggg ccctggcccg
     2761 cgtcggcagc atcgtcgcgc tggtcggggt cggcgctttc gtctatttcg cgctcgcctg
     2821 gctcaccgga gcgatcgacc gcaacaagat cgccatgctg acccgcaggc aagccaagtc
     2881 caactgagta aggttcccat gcgtatcgtt tccggcatcc agcccacggg caatctccac
     2941 cttggcaatt acctgggggc gatccgcaat tgggtgaaga tgcaggatga tgtcgccgcc
     3001 ggcggcgggc agagcctcta cttcctggct gaccttcacg ccatttcgat gccgcacagc
     3061 ccggccgaac tgcgcgagaa cacccttgag atgaccgcgg cgttggtcgc ctgcgggatc
     3121 gatcccgaca agtcggtcct gttcaaccag gcccaggttc cggcccatgc cgagctgcaa
     3181 tggctgctgt ccggcaccgg ccggatgggc tggctgaacc ggatgaccca gtggaaggac
     3241 aaggccggga agaaccgtga aggccagtcg gtcgcgctgt tcacctaccc tgtgctccag
     3301 gctgctgacg tgctgctgta ccaggccacc cacgtgccgg tgggcgagga ccagaagcag
     3361 cacctggagc tggcccgtga catcgcgcag aagttcaaca acgacttcgc gagcgaggac
     3421 gccccggtct tcaccctgcc cgatccgatc atcccgcccg aagcggcccg gatcatgtcc
     3481 ctgcgcgacg gcagcgccaa gatgagcaag agcgatccga gcgacatgag ccggatcaac
     3541 ctgaccgacg atgccgacac gatcatgcag aaggtcaaga aggccaagac cgatcccgag
     3601 ccgctgcccg gcgacaaggg cggactggac ggccggccgg aagcggccaa cctggtcggg
     3661 atctatgccg cgatggccgg tgaaagccag gacgcggtgc tggcccgctt cggcggcgaa
     3721 ggctttggca agttcaagcc cgcgctgggc gagctgctgg tcgaaagcct ggccccgatc
     3781 aacgcccggt tcctggacct gcggcaggac cgcgaggcgc tcgacgcgat cctcgccaag
     3841 ggcg
//
LOCUS       DASUDV010000445         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2003172,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000445 DASUDV010000000
VERSION     DASUDV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003172"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV30_09665"
     CDS             <1..261
                     /locus_tag="VFV30_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX4848400.1"
                     /translation="IVVAILALHGLEIWLFAFVYMGTGAIAGLEDALYFSTISYSTVG
                     YNDTHIDPAWRLLGAFQSILGMVLIGWSTAFFFRMLGRIEAH"
     gene            complement(280..1554)
                     /locus_tag="VFV30_09670"
     CDS             complement(280..1554)
                     /locus_tag="VFV30_09670"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX4848401.1"
                     /translation="MRHPLLLTGTIAASFLLATALPAKPVQTPEQVAMAALAAAPVWD
                     GHNDIPDSLRDRRKNVLSGFDFTDTTGSADPARGFAALHTDLKRLRQGKVGAQFWSVY
                     VSASHSDPQAVQAALEQIDLTRRLIALYPNDLQYCADSACVEAAWKKGKIASLMGMEG
                     GHAIGGSLAVLRQMYAVGARYMTLTHSKNNAWADSATDAPVHNGLTPLGKDVVREMQR
                     MGMLVDISHVSEKTMLDVLAIAKAPVIASHSNAFAINPHPRNVPDSALDALKANGGIV
                     MVNFYPPFVLDAARIWGANKDAEEARLKTLHRGNPARAKAALDAWIQANPMPRGTVKD
                     VADHIDYIAKRIGVDHVGLGGDFDGIDVAVTGLESVADYPALFIELARRGWSQQDLEK
                     LASRNMMRVLKAAEAYAATQRGTPPFENPTSF"
     gene            complement(1586..>2027)
                     /locus_tag="VFV30_09675"
     CDS             complement(1586..>2027)
                     /locus_tag="VFV30_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848402.1"
                     /translation="RVGKRLGIPWYFVAATHGLEASFNFRAHLHNGDFPLTQRTRQVP
                     AGRPSPWLPPSDWESSAIDAMRLLGFAGQEDWSLPRCLYRLEAYNGFGYRRAGRPSPY
                     LWSFSTLYTRGKFVADGRFDPKARSKQCGAAVMIKLLDLAGELG"
BASE COUNT          296 a          667 c          705 g          359 t
ORIGIN      
        1 atcgtggttg ccatcctggc gctccacggg ctggaaatct ggctgtttgc cttcgtctat
       61 atgggcaccg gggcgattgc cgggctggag gacgcgctct acttctcgac catttcctat
      121 tcgacggtgg gctacaacga cacccatatc gatcccgcct ggcggctgct gggcgcgttc
      181 cagtcgatcc tcggcatggt cctgatcggc tggtcgaccg cgttcttctt tcgcatgctg
      241 ggccggatcg aggcgcacta gccgcgccgc gccgggtgct cagaagctgg tcgggttctc
      301 gaacggcggc gtgccgcgct gagtggcggc ataggcttcg gccgctttca gcacccgcat
      361 catgttgcgg ctggcgagct tttcgagatc ctgctggctc cacccgcggc gggccagttc
      421 gatgaacagc gccggatagt cggccaccga ttccagcccg gtcacggcca cgtcgatccc
      481 gtcgaaatcg ccgcccaggc cgacatggtc cacgccgatc cgcttggcga tgtaatcgat
      541 gtgatcggcc acgtccttca ccgtgccgcg cggcatcggg ttggcctgga tccacgcgtc
      601 gagcgcggcc ttggcccggg ccggattgcc gcggtgcagc gtcttgaggc gggcttcctc
      661 ggcgtccttg ttggcgcccc agatccgcgc cgcatccagc acgaagggcg ggtagaagtt
      721 gaccatcacg atcccgccgt tggccttcag cgcgtcgagc gcgctgtcgg gcacgttgcg
      781 ggggtggggg ttgatcgcaa aggcgttgga atggctggcg atgaccggcg ccttggcgat
      841 ggccagcacg tcgagcatgg tcttttcgct gacgtggctg atatcgacca gcatgcccat
      901 ccgctgcatt tcgcggacca cgtccttgcc cagcggggtc agcccgttgt ggaccggggc
      961 gtcggtggcc gaatccgccc aggcgttgtt cttcgaatgg gtgagcgtca tgtagcgcgc
     1021 gccgaccgcg tacatctggc gcagcacggc caggctgccg ccgatcgcgt ggccgccttc
     1081 catgcccatc agcgaggcaa tcttgccctt cttccacgcc gcctcaacgc aggccgaatc
     1141 cgcgcagtac tgcagatcgt tgggatagag cgcgatcagt cggcgggtca ggtcgatctg
     1201 ttccagcgcg gcctggaccg cctggggatc ggaatggctg gccgagacat agaccgacca
     1261 gaactgcgcc ccgaccttgc cctggcgcag ccgcttcaga tcggtgtgca gcgcggcaaa
     1321 gccgcgggcc ggatcggcgc tgccggtggt gtcggtgaag tcaaacccgc tcagcacgtt
     1381 cttgcgccgg tcgcgcagcg aatccggaat gtcgttgtgc ccgtcccaca ccggcgcggc
     1441 ggccagcgcg gccatggcaa cctgttccgg ggtctgcacc ggcttggccg gaagcgcggt
     1501 ggcaagaagg aacgaggccg cgatcgtgcc ggtcagcaaa agggggtggc gcatggcgta
     1561 tcctgtggtt gggcgggcgg ccagcctagc caagctctcc ggccaggtcg agcagcttga
     1621 tcatcaccgc cgcgccgcac tgcttggacc gggccttggg atcgaaccgg ccgtcggcca
     1681 cgaacttgcc ccgggtgtag agcgtcgaga agctccacag ataggggctg ggccgtccgg
     1741 cccggcgata gccaaagccg ttgtaggcct ccagccggta gaggcagcgc ggcaggctcc
     1801 agtcctcctg gcccgcaaag ccgagcagcc gcatcgcgtc gatcgcgctc gattcccagt
     1861 cgctgggcgg cagccacggg ctgggccgcc cggccgggac ctggcgggtg cgctgggtca
     1921 gcgggaaatc gccgttgtgg aggtgggcgc ggaaattgaa gctggcctcc agcccgtggg
     1981 tcgcggcgac gaagtaccac gggatgccca gccgcttgcc gacccgc
//
LOCUS       DASUDV010000446         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1958763,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000446 DASUDV010000000
VERSION     DASUDV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958763"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFV30_09680"
     CDS             complement(<1..618)
                     /locus_tag="VFV30_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX4848403.1"
                     /translation="MTRRIIPEAIAREPWHVLLPLFALNTFGSAVLHSAAGGHMDPWA
                     LLHTIHFAVFLVVAFVISRLPREFFKAIAYPVFIVLIVALALVDLVGSVRGGSQRWLN
                     LGFMMFQPSEPMKLSIVLALAHFFDSLPPAITRSWRVMLPLSALVGLPVLLVMLQPDL
                     GTGLSIMAGAVVVVFMAGLPLRWFIGAGAAGVAIAPVAFFTLLHDY"
     gene            complement(615..>2473)
                     /gene="mrdA"
                     /locus_tag="VFV30_09685"
     CDS             complement(615..>2473)
                     /gene="mrdA"
                     /locus_tag="VFV30_09685"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX4848404.1"
                     /translation="RMEYRIDIIPERLVDAKGTVAKLGTLLGLDAIAMRDLSDKLEKA
                     HGFQPLGVASGIPPEVYDKVLVRLPEMPGVVAAQGFTRWYPTGPSVGHLLGYVGPANA
                     EEYKAERNPLLVTPGFKIGKDGFEKFFEAQLRGTPGARRAEVTAGGKIVRDLEMREDV
                     PGQSLTLTINGPLQDYAARRLGLESGAVVVMDCENGDILAMASMPSFDPNSFSDGIGR
                     IEWKMLSDDDHVPLRNKVLRGLYPPGSTVKPAVAMAFLEAGLSPDASVNCGGGLRVGN
                     RVFGCWNRRGHGSVNMAKAIYQSCDVYFYHFAQKIGMDAIAAMVRRLGLGQKFDLPVV
                     GQHFGTVPDPAWKQKKYGKPWAVFDTVNATIGQGYMLVNPLQQAVMAARIASGKLLQP
                     RLLLDRNTPPAPSMGFSQEHLDYVHKAMSDVVNGPGTAGRARLPIPDVLLAGKTGTAQ
                     VVGLNISDGKSGPWKYRDHGHFICYAPFDKPRYACAVVVEHGGGSASAYPIARDVMTF
                     LYDPAKALEVLHGMESGWGGTPQERMATRYRSYANKYGVNAPKPADEQEIERQSQSTN
                     PDAAEPRSPATEAATPRADPDAEASSAPAAPAATGAAPAPGPAAPAASGTPQ"
BASE COUNT          407 a          836 c          850 g          380 t
ORIGIN      
        1 gtaatcgtgc agcagcgtga agaaggccac cggagcgatg gccaccccgg ccgcgcccgc
       61 gccgatgaac cagcgcagcg gcaggccggc catgaacacc accaccaccg cgccggccat
      121 gatcgacagg ccggtgccaa ggtccggttg gagcatgacc agcagaaccg gaaggccaac
      181 cagcgccgac agcgggagca tcacccgcca gctgcgggtg atggccggcg gcagactgtc
      241 aaagaaatgg gccagggcca gcacgatcga cagcttcatc ggttcggacg gctggaacat
      301 catgaagccc aggttgagcc agcgctggct gccgccgcgc acgctgccga ccagatcgac
      361 cagcgccagg gcgacgatca ggacgatgaa caccggatag gcgatcgcct tgaagaactc
      421 gcgcgggagg cgcgagatca cgaaggccac gaccaggaag accgcgaagt ggatcgtgtg
      481 gagcagggcc cagggatcca tgtgcccgcc cgccgccgaa tggagcacgg ccgagccaaa
      541 ggtgttgagc gcgaacagcg gcagcagcac gtgccacggc tcgcgcgcga tcgcctcggg
      601 gatgatgcgg cgggtcattg cggcgtcccg ctggcggccg gtgcggccgg gccgggggca
      661 ggggccgctc cggttgcggc cggcgcggcg ggagcggagg acgcctccgc atccggatcg
      721 gcgcgcgggg tggccgcttc ggttgccgga ctgcgcggct ccgcagcatc cgggttggtg
      781 ctttgcgact gccgctcgat ttcctgctcg tcggccggct tgggggcgtt gaccccgtac
      841 ttgttcgcat agctgcgata gcgcgtcgcc attcgctcct gcggggtgcc gccccagccg
      901 gattccattc cgtgcagcac ttcgagcgcc ttggccggat cgtagaggaa ggtcatcacg
      961 tcgcgcgcga tcggataggc cgatgcggag ccgccgccgt gctccaccac caccgcgcag
     1021 gcatagcgcg gcttgtcgaa cggggcgtag cagatgaagt ggccgtgatc gcggtacttc
     1081 cacggaccgg acttgccgtc cgagatgttg aggccgacga cctgcgccgt cccggtcttg
     1141 ccggccagca gcacatcggg gatcggcaga cgcgcccgcc cggcggtgcc ggggccgttc
     1201 accacgtcgc tcatcgcctt gtggacgtaa tcgaggtgtt cctggctgaa gcccatgctg
     1261 ggcgccggcg gagtgttgcg gtccagcagc agccggggct ggagcagctt gcccgaggca
     1321 atccgcgccg ccatcaccgc ctgctgcagc gggttcacca gcatgtagcc ctggccgatg
     1381 gtggcgttga cggtatcgaa caccgcccag ggcttgccgt atttcttctg cttccaggcg
     1441 ggatcgggca cggtgccgaa atgctggccc accaccggca gatcgaactt ctggccgagg
     1501 cccaggcgcc gcaccatcgc cgcgatcgcg tccatcccga tcttctgggc gaagtgatag
     1561 aaatagacgt cgcagctctg gtaaatcgcc ttggccatgt tgaccgagcc gtggccgcgg
     1621 cggttccagc agccgaacac ccggttgccg acccgcaggc cgccgccgca attgacgctg
     1681 gcatcggggg aaagcccggc ctccagaaag gccatggcca ccgccggctt taccgttgac
     1741 ccgggcggat agagcccgcg cagcaccttg ttgcgcagcg ggacgtgatc gtcgtccgac
     1801 agcatcttcc attcgatccg gccgatgccg tcggaaaagc tgttgggatc gaaactgggc
     1861 atcgaggcca tggccaggat gtccccgttc tcgcagtcca tcaccacgac cgcgccgctt
     1921 tccagcccga gccgccgcgc ggcatagtcc tgcagcgggc cgttgatcgt cagggtcagc
     1981 gactggcccg gcacgtcctc gcgcatttcc agatcgcgga cgatcttgcc gcccgcggtc
     2041 acttccgcgc gccgcgcccc cggggtgccg cgcagctggg cttcgaagaa cttctcgaac
     2101 ccgtccttgc cgatcttgaa gccgggggtg accagcaacg gattgcgctc ggccttgtac
     2161 tcctcggcgt tggccgggcc gacatagccg agcaggtggc cgaccgaggg tccggtcgga
     2221 taccagcggg taaagccctg ggcggcgacc acgcctggca tttccggcag gcggaccagc
     2281 accttgtcat agacttccgg cgggatgccc gatgccacgc caaggggctg gaagccgtgg
     2341 gccttctcca gcttgtcgga caggtcgcgc atcgcgatgg cgtccagacc cagcagcgtg
     2401 cccagcttgg ccacggtgcc cttggcatcg accagccgct cggggatgat gtcgatccgg
     2461 tattccatcc ggt
//
LOCUS       DASUDV010000447         7414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1362828,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000447 DASUDV010000000
VERSION     DASUDV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362828"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFV30_09690"
     CDS             complement(<1..297)
                     /locus_tag="VFV30_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4848405.1"
                     /translation="MAENLLIQRDSYFTAAQEDRSAPRTRITIPATLRVSGSRGFQTV
                     VHDLSLSGFCAAAVNRLHPGTVCWLTLPGIEALQGEVIWWDNSLTGCAFDKLLSP"
     gene            complement(383..1096)
                     /locus_tag="VFV30_09695"
     CDS             complement(383..1096)
                     /locus_tag="VFV30_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4848406.1"
                     /translation="MTALSGKIALVTGASRGIGAAIAEALAAAGAHVVLTGRDTRQLE
                     TVEQRIFEAGGSATIAPVDLADADGIARLANAVAERWHRVDVLVHSAAVLPDLMAAKD
                     IDQVQFSKALTVNVLATQALISRFDALLRASEDPRLVYLTTSVATAPRAYWGAYAASK
                     AAAEVLIDCYAQETRNLGKLKVAIVDPGATRTAMRAKAYPGEDPASVKLPSVVADRVV
                     AMLGEQIASPHRERINQSS"
     gene            complement(1093..2565)
                     /gene="purF"
                     /locus_tag="VFV30_09700"
     CDS             complement(1093..2565)
                     /gene="purF"
                     /locus_tag="VFV30_09700"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX4848407.1"
                     /translation="MNLTHPFLDADGDKLREECGIFGVIGTKEAAAMTALGLHALQHR
                     GQEAVGIVSYDGKDFFARRGLGHVAQVFFDTASLAELPGEMASGHVRYSTTGGQGLRN
                     VQPLHADLARGAFSVAHNGNISNAMHLKQELVSKGAIFQSTSDTEVILHLIATSRYPT
                     LMDRFVDALRMIEGAYSLICMTPKRMIACRDPLGIRPLVMGKIGDATVFASETVALDV
                     VGADFVREVEPGEIIEVRQDGRMSSHRPFGSPSPRPCIFEHVYFSRPDSVMGGQSVYE
                     VRKAIGMELAKEAPVEADLVIPVPDSGVPAAIGYAQESGIPFELGIIRSHYVGRTFIQ
                     PSDGARHADVKRKHNANRALVEGKRIVLIDDSIVRGTTSMKIVQMMRDAGAAEVHMRV
                     ASPPTMHSCFYGVDTPERHKLLAARMDVQEMTKFIVADSLAFVSIDGLYRAVGEAKRN
                     AACPQHCDACFTGEYPTSLTDLAERVADADQLPLPVGQVA"
     gene            2752..3591
                     /locus_tag="VFV30_09705"
     CDS             2752..3591
                     /locus_tag="VFV30_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4848408.1"
                     /translation="MQPRILSALAAISLISAPAALHAAKPAEVELHIEASASVQPDRA
                     EVPLTIRASDLSEAEALAALAETEQRLRKDLEREGIRPDQIKVQPGANGKDVAITSDD
                     VTTACAAADAAADVAADAAAASDMDAPPAARKRSPAKRDAPQREACPNEYTAAKTLLV
                     TVTGDMAVVDRLIVMGNESEYGFVRMRPVYSQSDPAAARAKARSEALAKAQAEGTAYA
                     DAMGYRVVRIVRVSNARPAMSMNDLIAFIAAAETRSGAMQPSWFAATVTESVAIDFVM
                     VPK"
     gene            3682..4923
                     /locus_tag="VFV30_09710"
     CDS             3682..4923
                     /locus_tag="VFV30_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX4848409.1"
                     /translation="MLLTPFEWTIAKRYMLPGRGEAFIALVAGISLVAVMLGVAALVI
                     VMSVMNGFRAELFDKIVGLNGHAIVQAYGGRLENWREVLKEVRATPGVTSASPLIEQP
                     LMGTFNGRVDLVFARGNTAEDIAALKPKVVAGRIEELTPESGNIALGAQLASNLGIRV
                     GDSLTIINPQGQATPFGTVPRQVSYRVAAIFEIGVFDFDERFAVIPLADAQTLLLSGD
                     TVGMIEVQVTDPERAKEILAPVAERLAGQAVVVDWKAMNASLFEALAVERVAMFVVLS
                     IIVLVAVFNILSSLIMLVRAKTRDIAILRTMGATRRSILKIFVTLGFVIGSAGTLAGL
                     ILGFVFLYFRQSLVRGIEIITGQNLWDPSIRFLTELPSRSDPVEIAIISVMALVFSFL
                     ATLYPARKAASTDPVQVLRYE"
     gene            4916..5590
                     /locus_tag="VFV30_09715"
     CDS             4916..5590
                     /locus_tag="VFV30_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4848410.1"
                     /translation="MNSPVVELTGLTRSFEQGGVRIDVLRGVNLTVAPGEIVALLGPS
                     GSGKSTLLQAVGLLEGGFGGRIAIAGQDASALDGDARTVLRRERIGFVYQFHHLLADF
                     NATENVVLPQLVAGTAQAEAEARAHELLTALGLGERLDHRPSQLSGGEQQRVAVARAL
                     ANRPALVLADEPTGNLDEKTADRVLEQFLELVRGTGSAALVATHNERLAARMDRVVRL
                     HEGVLE"
     gene            5590..6132
                     /locus_tag="VFV30_09720"
     CDS             5590..6132
                     /locus_tag="VFV30_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848411.1"
                     /translation="MALTGALALALLAAQAGTADPVPVVPLPPADTCTGPLYTAFDFW
                     VGQWNVFRKADGQQIARSAVAKINGDCALREDWLPLDGKPGGTLSAPDMITGRWHHYW
                     LDGKGARVDLEGGLYYGEMILAGTWRGGGPGGADVLVRVTFTRLDADSVRQRGEMSED
                     EGLTWTPAFDYLYLRRTAAD"
     gene            6185..6664
                     /locus_tag="VFV30_09725"
     CDS             6185..6664
                     /locus_tag="VFV30_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX4848412.1"
                     /translation="MTTIADFTADLPGGKQINLADKAGKVLLVVNTASKCGFTPQYDG
                     LEALHQKYGAQGFEVLAFPCNQFGGQEPGSADQIEEFCKVNFGLSFPLMAKVEVNGPN
                     EPALYSWLKSEAPGLMGTKSIKWNFTKFLIDRQGKVVRRYAPTDKPESIAKDIEKLL"
     gene            complement(6687..>7414)
                     /locus_tag="VFV30_09730"
     CDS             complement(6687..>7414)
                     /locus_tag="VFV30_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848413.1"
                     /translation="IGLYGLVLYFKGVTPLMKVPQDKALGFTAIAIVCAVILSVIAGT
                     VLGTVTGAMGAGAAAISGASGDKVEVNLPGVGKINTGKMDEATKQLEAMASGQPPKPV
                     DLAALQGLLPASIGGFQRTSIESAGVGGVGSKAEAVYRNGDKQVRVEIVDMAGLGVVA
                     GALGGLGVEQNREDANGYERTRTVDGAIQTEKWDTRRSRGSFGRQVAGRFMVMAEGDA
                     GSIEELKAIVAGIDPGKLADLAK"
BASE COUNT         1187 a         2464 c         2538 g         1225 t
ORIGIN      
        1 cggcgagagc agcttgtcga aagcgcagcc ggtcaggctg ttgtcccacc agatcacttc
       61 gccttgcagc gcctcgatcc cgggcagggt cagccagcac acggtgccgg gatgcagacg
      121 gttgaccgcg gcggcgcaga agccgctcag cgacaggtca tggaccacgg tctggaaacc
      181 gcgcgaaccc gacacccgca aggtggccgg aatggtgatc cgcgtgcgcg gcgcgctgcg
      241 gtcttcctgc gcggcagtga agtaggaatc tctctggatc agcagatttt cagccatcgt
      301 ctgcgcccct ctttgtgaca ggctttccgg agggtttccc gggaagcttt gcaaagtatg
      361 gcgccggtcg gttgcccggg acttacgagg actggttaat cctttcgcgg tgcgggctgg
      421 caatctgttc accaagcatc gcaaccacgc ggtccgccac caccgacggc agcttgaccg
      481 aagcggggtc ttcgcccgga taggccttgg cccgcatcgc ggtgcgggtg gcgccgggat
      541 cgacgatcgc aaccttcagc ttgcccagat tgcgggtttc ctgggcgtag caatcgatca
      601 gcacttcggc cgcggccttg ctggcggcat aggcgcccca ataggcgcgc ggggccgtgg
      661 cgacgctggt ggtcaggtaa accagccgcg ggtcctcgct ggcgcgcagc agggcgtcga
      721 aacgggaaat cagggcctgg gtggccagca cgttgacggt cagcgccttc gagaactgga
      781 cctggtcgat atccttggcc gccatcaggt cgggcagcac cgcggcgctg tggaccagca
      841 cgtcgacccg gtgccagcgt tcggccacgg cgttggccag ccgggcgatc ccgtcggcat
      901 cggccagatc gaccggcgcg atggtggcgc tgccgccggc ctcgaagatc cgctgttcga
      961 cagtttccag ctggcgggtg tcacggccgg tcagcaccac gtgggcgccg gcggcggcca
     1021 gggcctcggc aatggccgca ccgatcccgc ggctcgcccc ggtgacgagc gcgatcttgc
     1081 ccgacagcgc ggtcacgcga cctggccgac cggcagcggc agctggtccg catcggccac
     1141 gcgttcggcc agatcggtca ggctggtggg gtattcaccg gtaaagcagg cgtcgcagtg
     1201 ctgcgggcag gcggcgttgc gcttggcctc gccgaccgcg cggtacaggc cgtcgatcga
     1261 aacgaaggcc aggctgtccg ccacgatgaa cttggtcatc tcctgcacgt ccatccgcgc
     1321 cgccagcagc ttgtgccgtt cgggcgtatc gaccccgtag aagcaggaat gcatggtcgg
     1381 cgggctggcg acgcgcatgt gcacttcggc cgcgccggca tcgcgcatca tctgcacgat
     1441 tttcatcgag gtggtgccgc gcacaatcga atcgtcgatc agcacgattc gcttgccctc
     1501 caccagcgcg cggttggcgt tatgcttgcg cttcacgtcc gcgtggcggg cgccgtcgct
     1561 gggctggatg aaggtgcgcc ccacatagtg cgaacggatg atccccagtt caaagggaat
     1621 gccgctttcc tgggcatagc cgatcgcggc gggaacgccg ctgtcgggca ccggaatcac
     1681 caggtcggcc tcgaccgggg cttccttggc cagttccatc ccgattgcct tgcgcacttc
     1741 gtagaccgac tggccgccca tcaccgaatc cggacggctg aaatagacgt gttcgaaaat
     1801 gcacgggcgc ggggaagggc tgccgaaggg gcggtggctg gacatccggc catcctggcg
     1861 cacttcgatg atctcgccgg gttcaacctc gcgcacgaaa tccgcgccga ccacgtcgag
     1921 cgccacggtt tcgctggcaa agacggtggc atcgccgatc ttgcccatca ccagcgggcg
     1981 gattcccagc ggatcgcggc aggcgatcat ccgcttgggc gtcatgcaga tcagcgaata
     2041 ggccccctcg atcatccgca gcgcatcgac gaagcggtcc atcagggtgg gatagcggct
     2101 ggtcgcgatc aggtgcagga tcacttcggt gtcagaggtc gactgaaaga tcgcgccctt
     2161 gctgaccagc tcctgcttga ggtgcatcgc gttggaaatg ttgccgttgt gcgccaccga
     2221 aaaggcgccg cgggccagat ccgcgtggag cggctggacg ttgcgcaggc cctggccgcc
     2281 ggtggtcgaa tagcggacat ggccgctggc catttcgccc ggcagttcgg ccagcgaggc
     2341 ggtgtcgaaa aagacctggg cgacatggcc cagaccgcgc cgggcaaaga agtccttgcc
     2401 gtcatagctg acgatgccga ccgcttcctg tccgcggtgt tgcagggcgt gcagccccag
     2461 ggcggtcatc gcggcggctt ccttggtgcc gatgacaccg aagatgccgc attcctcgcg
     2521 cagcttgtcg ccgtctgcgt caaggaaggg gtgggtaagg ttcatggtga gtcgcctggc
     2581 ccttgctgtg ccttcgtggc tgcgcctttg gcggggattg cgccccttgg caagggcgcg
     2641 gcggcctgca ctcaacaagc atctgatttg cacgcgattt ggcttgcaac gcggggcgac
     2701 ttgcgggcta atcgcaacgg cccgttcggg gcgagagaga ggggatcacc catgcagcca
     2761 cgcatcctgt ccgccctggc ggcgatcagc ctgatcagcg cgcccgccgc gctccacgcc
     2821 gccaagcccg ccgaggtgga gctgcacatc gaagcgagcg cctccgtcca gcccgaccgg
     2881 gccgaagtgc cgctgacgat ccgggcctcc gacctgagcg aggccgaagc cctggccgcc
     2941 ctggccgaga ccgagcagcg gctgcgcaag gatctggaac gggaagggat tcgcccggac
     3001 cagatcaagg ttcagcccgg cgcaaacggc aaggatgttg ccattacctc ggacgatgtc
     3061 accactgcct gcgccgcggc ggatgccgca gcggatgttg cggccgatgc ggcagccgct
     3121 tcggatatgg acgctcctcc cgcagcccgc aaacgcagcc cggcgaagcg ggatgcgccg
     3181 cagcgcgagg cctgtcccaa cgaatacacc gctgcaaaga ccctgcttgt cacggtgacg
     3241 ggcgacatgg ccgttgtgga tcggctgatc gttatgggca acgagagcga atacgggttc
     3301 gtccggatgc gccccgtcta cagccagagc gatcccgcgg ccgccagggc caaggcccgg
     3361 tccgaagccc tggccaaggc ccaggccgag ggcacggcct acgccgatgc gatgggttac
     3421 cgggtggtgc ggatcgtacg ggtcagcaac gcccggccgg ccatgtcgat gaacgatctg
     3481 atcgccttca tcgccgcggc ggaaacccgg tccggggcga tgcagcccag ctggttcgcc
     3541 gcgacggtga ccgaatcggt cgcgatcgat ttcgtgatgg tgcccaagta atcgggcgca
     3601 cgggtgccgg cggccttgcc cttggggcgg ggggcgccta aatcccggcg caagttccct
     3661 ccggcgctgt ggcgaggatc cgtgctgctt acccctttcg aatggaccat cgccaagcgc
     3721 tatatgctgc ccggccgggg cgaggcgttc attgcgctgg tcgccgggat cagcctggtc
     3781 gcggtgatgc tgggcgttgc cgcgctggtc atcgtgatga gcgtgatgaa cggctttcgc
     3841 gccgaactgt ttgacaagat cgtcgggctt aacgggcacg ccatcgtcca ggcctatggc
     3901 ggccggctcg aaaactggcg tgaggtgctg aaggaggtcc gcgccacgcc gggggtcact
     3961 tcggcctcgc cgctgatcga acagccgctg atgggcacct tcaacggccg ggtcgatctg
     4021 gtctttgccc gcggcaacac ggccgaggat atcgccgcgc tgaagcccaa ggtggtggcc
     4081 gggcggatcg aggaactgac gcccgagagc ggcaacatcg cgctgggcgc gcagctggcc
     4141 agcaacctgg ggatccgggt gggtgacagc ctgaccatca tcaacccgca gggccaggcc
     4201 actccgttcg gcaccgtgcc gcgccaggtg tcctaccgcg tcgccgcgat cttcgagatc
     4261 ggggtgttcg atttcgacga gcggttcgcg gtgatcccgc tggccgatgc ccagaccctg
     4321 ctgctcagcg gcgataccgt gggcatgatc gaagtgcagg tgaccgatcc cgaacgggcc
     4381 aaggaaatcc tggccccggt ggccgagcgg ctggccgggc aggccgtggt ggtggactgg
     4441 aaggcgatga acgccagcct gttcgaagcc ctggcggtgg aacgggtggc gatgttcgtg
     4501 gtcctgtcga tcatcgtgct ggtggcggtg ttcaacatcc tctccagcct gatcatgctg
     4561 gtccgcgcca agacccgcga catcgcgatc ctgcggacaa tgggcgccac ccgccgctcg
     4621 atcctgaaga tcttcgtgac cctgggtttc gtgatcggtt cggccgggac cctggccggg
     4681 ctgatcctgg gcttcgtgtt cctttatttc cggcaaagcc tggtccgcgg gatcgagatc
     4741 atcaccggcc agaacctgtg ggatccctcg atccggttcc tgaccgagct gcccagccgc
     4801 agcgatccgg tggaaatcgc gatcatcagc gtcatggccc tggtcttcag cttcctcgcc
     4861 acgctctatc cggcgcgcaa ggcggcctcg accgatccgg tgcaggtgct gcgctatgaa
     4921 tagccccgtc gtcgaactga ccggcctgac ccgcagcttt gaacagggcg gggtgcggat
     4981 cgacgtgctg cgcggcgtca acctgaccgt cgcgccggga gagatcgtcg cgctgcttgg
     5041 cccctcgggt tcgggcaagt ccaccctgct gcaggccgtg ggcctgctcg aaggcgggtt
     5101 cgggggccgg atcgcgattg ccgggcagga tgccagcgcg ctggacggcg atgcccgcac
     5161 cgtgctgcgg cgggaacgga tcggctttgt ctatcagttc caccacctgc tggccgattt
     5221 caacgccacc gaaaatgtcg tcctgccgca gctggtggcc ggaaccgccc aggccgaagc
     5281 cgaggcccgc gcccacgagc tgctgaccgc gctggggctg ggcgagcggc tggaccaccg
     5341 gcccagccag ctgtccggcg gcgagcagca gcgcgtggcc gtggcccgcg ccctggccaa
     5401 ccgcccggcg ctggtcctgg ccgacgagcc gaccggcaac cttgacgaaa agaccgccga
     5461 ccgcgtgctg gagcagttcc tcgagctggt ccgcggcacc ggcagcgcgg cactggtcgc
     5521 cactcacaac gaacggctgg ccgcccggat ggaccgcgtg gtgcgcctgc acgagggcgt
     5581 gctggaataa tggccctgac cggcgccctt gcgctggccc tgctggcggc gcaggccggg
     5641 acggcggatc cggtcccggt cgtgccgctg ccgccggcag atacctgcac cgggccgctc
     5701 tataccgcgt tcgatttctg ggtcgggcag tggaacgtgt tccgcaaggc cgatgggcag
     5761 cagatcgccc gttccgccgt ggccaagatc aacggcgatt gcgcgctgcg cgaagactgg
     5821 ctgccgctgg acggcaagcc cggcggcacg ctcagcgcgc ccgacatgat caccgggcgc
     5881 tggcatcact actggctgga cgggaagggc gcgcgggtcg atctggaagg cgggctctat
     5941 tacggcgaga tgatcctggc cggcacctgg cgcggcggcg ggccgggcgg ggcggacgtg
     6001 ctggtgcggg ttacctttac ccggctcgat gcggattcgg tccgccagcg gggcgaaatg
     6061 tcggaggacg aagggctgac ctggaccccg gcgttcgatt acctctacct gcgccgcacc
     6121 gccgccgatt gacgcggccc cgcgccgcac tagggtgcgg gcaaagccat ccggggagac
     6181 caccatgacc acgattgccg atttcaccgc cgacctgccc ggcggcaagc agatcaacct
     6241 ggccgacaag gccggcaagg tgctgctggt ggtcaacacc gccagcaagt gcggcttcac
     6301 cccgcagtac gacgggctgg aggcgctgca ccagaagtac ggcgcgcaag gctttgaagt
     6361 gctggccttc ccctgcaacc agttcggcgg acaggaaccc ggatcggccg atcagatcga
     6421 ggaattctgc aaggtcaatt tcggcctgtc gttcccgctg atggccaagg tcgaagtcaa
     6481 cggcccgaac gagccggcgc tctattcctg gctgaagagc gaggcccccg ggctgatggg
     6541 cacgaagtcg atcaagtgga acttcaccaa gttcctgatc gatcgccagg gcaaggtcgt
     6601 gcgccgctat gccccgaccg acaagcccga gagcatcgcc aaggacatcg aaaagctgct
     6661 gtaggttcct cgcctgaggg gaaggatcac ttggccaggt cggccagctt gcccggatcg
     6721 atcccggcga cgatcgcctt cagctcctcg atcgagccgg catcgccttc ggccatgacc
     6781 atgaagcggc cggccacctg gcgaccaaag ctcccgcggg agcggcgggt gtcccacttc
     6841 tcggtctgga tcgcgccgtc gacggtgcgg gtccgttcat agccgttggc gtcctcgcgg
     6901 ttctgttcca cccccaggcc gcccagcgcc ccggccacca cgcccagccc ggccatgtcc
     6961 acgatctcga cccggacctg cttgtccccg ttgcggtaga ccgcctcggc cttggagccg
     7021 actccgccga ccccggcgga ttcgatcgag gtgcgctgaa agccgccaat cgaggccggc
     7081 agcagcccct gcagcgcggc gaggtcgacc ggcttgggtg gctggccgct ggccatggct
     7141 tccagttgct tggtcgcctc gtccatcttg ccggtgttga tcttgcccac gccggggagg
     7201 ttcacttcca ccttgtcccc cgatgcgccg ctgatcgcgg ccgcgcccgc gcccatcgcg
     7261 ccggtgaccg tgccgagcac cgtgccggcg atcacgctga ggatcaccgc acagacgatc
     7321 gcgatcgcgg taaagcccag ggccttgtcc tgcggaacct tcatcagcgg ggtcacgccc
     7381 ttgaaataga gcaccagccc gtagagcccg atca
//
LOCUS       DASUDV010000448         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_655960,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000448 DASUDV010000000
VERSION     DASUDV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655960"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="glmS"
                     /locus_tag="VFV30_09735"
     CDS             <1..1422
                     /gene="glmS"
                     /locus_tag="VFV30_09735"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX4848414.1"
                     /translation="VEGGASPEEAIKATLPQLRGAFALAIAFKDYPDMLIGARLGSPL
                     VLGYGEGETYLGSDALALAPLTQQICYLEEGDWVVVRRDGAQIFDSANQPVTRPVTTS
                     GASAAAIEKGNYRHFMQKEIFEQPTVVAQTLKSYIRQVDQSVALPQFDMDLSKISRVT
                     IVACGTSFYAGMVAKYWLEQFARLPVDIDVASEFRYRDPVLEPGGLALFISQSGETAD
                     TLAALRHCKAAGQTIAVVVNVPTSSMAREADLLLPTHAGPEIGVASTKAFTCQLAVLA
                     AFAAHMAVKRGRMSRAEEADVVAHLLEAPASLNAALAHDDDIAAMAHLIAPARDVLYL
                     GRGADYPLALEGALKLKEISYIHAEGYASGEMKHGPIALIDEAVPVIVLAPSGPLFEK
                     TVSNMQEVRARGGKIVLISDAEGLAEAGEGCLATIEMPKVHPLIAPLVYAVPVQLLAY
                     HVACVKGTDVDQPRNLAKSVTVE"
     gene            1419..1799
                     /locus_tag="VFV30_09740"
     CDS             1419..1799
                     /locus_tag="VFV30_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX4848415.1"
                     /translation="MKVHGGCHCGAVRFSAEVEPSPELLDCNCSICAKTGFLHLIVPH
                     ERFVLLQGDQALSTYKFGTGAARHLFCKHCGIKSFYQPRSHPEAWSISWRCLDPGHGL
                     EPRIIAYDGRNWEAARAALDREID"
     gene            1841..>2945
                     /locus_tag="VFV30_09745"
     CDS             1841..>2945
                     /locus_tag="VFV30_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4848416.1"
                     /translation="MSTDPTPSSALPKTLPVMAVLGLVDPADGDWGRLRGLQYSSLAR
                     VSQVRVLAHAIAALGVARLSFGEVHFAILLGWIALLGASLWHGAKIDKTLADADRRRI
                     SRDEVNNQSISSILNALVWTVPIAGSVAVGDANGQLQLWAVTAMLMTAAAVLLPAVPL
                     ANLLFTAIVGGCALVSFMFGGNIEMALVSLVFSIVVALGSIENARSFLIARIAEAGMA
                     EKNEVVSLLLREFEEGEADWLWQVDASRRVRSVSPRFAYALGIPSEEIDGKPFIQLIA
                     GEAWDTGQFPSSLHDLAERLKRRESFSNLLVRVTINEQQRWWELSGTPKLDENGNFDG
                     FRDPRDQERARVFDRAQRHDNRKHQRNQRHFDIA"
BASE COUNT          507 a          930 c          950 g          558 t
ORIGIN      
        1 gtcgaaggcg gagccagccc ggaagaggca atcaaggcca ccctgccgca attgcgcggc
       61 gcctttgccc tggcgatcgc cttcaaggat taccctgaca tgctgatcgg cgcgcggctc
      121 ggctcgccgc tggtgctcgg ctatggtgag ggtgagacct atcttggctc ggatgcgctg
      181 gccctggccc cgctgaccca gcagatctgt tatctcgaag agggcgactg ggtggttgtg
      241 cgccgtgatg gcgcgcagat cttcgatagt gccaaccagc cggtgacccg cccggttacc
      301 acctctggtg cgtcggccgc ggcgatcgag aagggcaatt accgccattt catgcagaaa
      361 gagatcttcg agcagccgac cgtcgtggcc cagacactca agagctatat ccgccaggtc
      421 gaccaatcgg tggcgctgcc gcagttcgac atggacctct cgaagatcag ccgggtcacc
      481 atcgtcgcct gcgggaccag cttctatgcc ggaatggtcg ccaagtactg gctcgaacag
      541 ttcgcgcggc tgccggtaga catcgatgtg gccagcgaat ttcgctatcg cgatccggta
      601 cttgaaccgg gcggtctcgc gctgttcatc tcgcagagcg gcgagaccgc cgatacgctc
      661 gccgcgctgc gtcactgcaa ggcagccggg cagaccatcg ccgtggtggt caacgtgccg
      721 accagttcga tggcgcgcga ggccgatctg ctgctgccga cccatgctgg tccggaaatc
      781 ggcgtggcca gcaccaaggc cttcacctgc cagcttgccg tgctggccgc ttttgccgcg
      841 cacatggcgg tcaagcgcgg gcggatgagc cgcgccgaag aggccgatgt agtcgcccac
      901 ttactcgaag cgcccgccag cctcaacgct gccctggcgc acgatgacga tatcgccgcg
      961 atggcacacc tcatcgcccc ggcgcgcgac gtgctctacc tgggacgcgg ggcggactat
     1021 ccgctggccc tcgaaggtgc gctcaaactc aaggaaatca gctatatcca cgccgaaggc
     1081 tatgcctcgg gcgaaatgaa gcacggcccc atcgcgctga tcgacgaagc cgtcccggtc
     1141 atcgtgctcg cgcccagtgg tccgctgttc gagaagaccg tgtcgaacat gcaggaagtg
     1201 cgggcgcgcg gcgggaagat cgtgctgatt tccgatgccg agggtctggc cgaagcgggc
     1261 gagggatgcc tggcaacgat cgagatgccc aaggtccacc cgttgatcgc cccgctggtc
     1321 tatgccgtgc cggtgcagtt gctggcctat cacgtcgcct gcgtgaaggg caccgatgtg
     1381 gatcagccgc gcaatctggc aaaatcggtg acggtggaat gaaggttcat ggcggctgtc
     1441 attgcggagc ggtgcggttt tcggccgagg tcgagccttc gcccgaactg ctcgactgca
     1501 attgctcgat ctgcgcgaag accggcttcc tgcacctgat cgtcccgcac gaacgctttg
     1561 tcctgcttca gggtgatcaa gcgctgagca cctacaagtt cggcaccggc gcggcgcgcc
     1621 accttttctg taaacactgc ggaatcaaga gcttttatca accacgctcg caccccgagg
     1681 cctggagcat atcctggcgc tgtcttgatc cggggcacgg acttgagccg cggatcatcg
     1741 cctacgatgg ccgtaactgg gaagctgccc gcgccgcgct ggaccgcgaa atcgactagc
     1801 ctttacgcgg tgctaacgat tggcgggtta tgccgcgctc gtgagcaccg acccgacacc
     1861 cagttccgcc cttcccaaga ccttgccggt catggccgtg cttggcctgg tcgatccggc
     1921 tgacggtgat tggggccgac tgcgcggcct gcagtattcc agccttgccc gggtcagcca
     1981 ggttcgggtt ctggcccatg ccatcgccgc gctgggcgtg gcccggctga gttttggcga
     2041 ggtgcatttc gcgattctgc tgggctggat cgccctgctg ggtgccagcc tgtggcacgg
     2101 tgcaaagatc gacaagaccc ttgccgacgc cgaccggcgc cggatttcgc gcgatgaagt
     2161 caacaaccag tcgatcagtt cgatcctcaa tgcgctggtc tggaccgtgc cgatcgccgg
     2221 ttcggtggcg gttggcgatg ccaacggcca gctgcagctg tgggcggtaa ccgcgatgct
     2281 gatgaccgcc gcagcggtgc tgctgccggc tgtgccgctc gccaatctgc tgtttaccgc
     2341 gattgtcggc ggctgtgcgc tggtcagctt catgttcggc ggcaatatcg aaatggcact
     2401 ggtttcgctg gtgttttcga ttgtcgtggc gctgggctcg atcgaaaacg cgcgttcgtt
     2461 cctgatcgcg cggatcgccg aagccgggat ggccgaaaag aacgaggtcg tctcgctgct
     2521 gctgcgcgaa ttcgaagagg gcgaggccga ctggctctgg caggtcgatg cctcgcgccg
     2581 ggtgcgttcg gtttcgccgc gctttgccta tgccctgggg attccgagcg aggagattga
     2641 cggcaagccg ttcatccagc tgatcgcggg cgaagcctgg gataccggcc agttcccgtc
     2701 aagcctgcac gatctggccg aacggctcaa gcgccgggaa agcttctcga acctgctggt
     2761 gcgcgtcacc atcaatgaac agcagcgctg gtgggagctt tcgggcaccc ccaagctcga
     2821 cgagaacggc aatttcgacg gcttccgcga tccgcgcgat caggaaagag cgcgcgtttt
     2881 cgatcgagcc cagcgccacg acaatcgaaa acaccagcga aaccagcgcc atttcgatat
     2941 tgccg
//
LOCUS       DASUDV010000449         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_324539,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000449 DASUDV010000000
VERSION     DASUDV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324539"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFV30_09750"
     CDS             <1..282
                     /locus_tag="VFV30_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX4848417.1"
                     /translation="KFTSRVLKHRPERIEVIYVDGPMRDLDNVWQFRPLEDGGCEVEF
                     MVSFTFRNAMFEKLAGQYFDRAFRKMVGAFETRAEALYGSNSSSATSAA"
     gene            complement(243..743)
                     /locus_tag="VFV30_09755"
     CDS             complement(243..743)
                     /locus_tag="VFV30_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX4848418.1"
                     /translation="MFPLNLLPDDLTQLAQRVIAANRETGRVVALAESCTGGLVCAAL
                     TEVAGSSAVLDRGFVTYSNEAKHELLGVSNDIIDAFGAVSIACAWAMAQGALARSKAD
                     VAVAITGIAGPDGGSEFKPVGTVIFARAVRGSDEIVAEERQFEAGSRAEVRRQAALVA
                     LELLLP"
     gene            complement(809..1954)
                     /locus_tag="VFV30_09760"
     CDS             complement(809..1954)
                     /locus_tag="VFV30_09760"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX4848419.1"
                     /translation="MAPAPTRSTAAVIVAAGQGLRAGQPLPKQFAPWRGKPVVRHSAE
                     ALAAAGVAPIVVAIPDGAADVAAAAVDGIPGVRLVTGGATRQESVRLALEALEGDAPA
                     RVLIHDAARPVLPQAVIERLLAALEADPGAIPVLPVVDSLTRAEGVRMGEPARREELR
                     RVQTPQAFRYPEILAAHRGWTGAADAGDDAQVARAAGLDVALVDGDEALHKLTFASDF
                     GAAAPAVRIGTGYDVHRLADGEELWLCGVKIEHPRGLAGHSDADVAIHALVDAILGAI
                     GSGDIGSHFPPSDPQWKGASSDRFLAHAVSLAAEAGYRIGNVDVTIICEAPKIGPHRE
                     AMRARLAGLLGTGTDAVSVKATTTERLGFTGRGEGIAAQAAATLLRG"
BASE COUNT          313 a          750 c          708 g          326 t
ORIGIN      
        1 aagttcacct cgcgcgtgtt gaagcatcga ccggagcgga tcgaagtgat ctacgtcgac
       61 ggcccgatgc gcgatctgga caatgtctgg caattccgcc cgttggagga tggcggctgc
      121 gaggtcgagt tcatggtcag cttcactttc cgtaacgcca tgttcgaaaa gctggctggg
      181 cagtatttcg atcgggcatt tcgaaagatg gttggcgcct tcgaaacgcg ggccgaggcg
      241 ctttacggca gcaacagctc cagcgcgacc agcgccgcct gacggcgcac ttcggcgcgc
      301 gatccggctt cgaactggcg ctcttcggcc acgatttcat cgctgccgcg cacggctcgg
      361 gcaaagatca cggtgccgac cggcttgaac tcgctgccgc cgtccgggcc ggcgatcccg
      421 gtgatcgcca ccgccacatc ggccttgctg cgggccagcg cgccttgcgc catggcccag
      481 gcacaggcga tcgagacggc gccgaacgca tcgatgatgt cgttcgaaac gccgagtagc
      541 tcgtgcttgg cctcgttcga ataggtcacg aacccgcggt cgagcacggc ggaggacccg
      601 gcgacctcgg tcagcgcggc gcagaccagt ccgccggtgc agctttccgc gagggcgacc
      661 acgcggcccg tctcgcggtt cgcggcaatc acgcgctggg ccagctgggt cagatcgtcg
      721 ggaagcaggt tcaagggaaa aatccgtccg ttgcgaatcg cgcgaagccc cttggtcccg
      781 tcgggtggga aaatccagcg ctattcgctc agccgcgcag cagggttgcc gcggcctggg
      841 cggcaatccc ttcgccccgg ccggtgaagc ccagccgctc ggtggtcgtc gccttcacac
      901 tgaccgcatc ggtcccggtg cccagcagcc cggccagccg cgcgcgcatg gcctcacggt
      961 gggggccgat cttgggggct tcgcagatga tcgtgacgtc gacattgccg atccggtagc
     1021 cggcctctgc cgccaggctg accgcgtggg ccaggaaccg gtcggaactg gcgcctttcc
     1081 actgcgggtc gctgggcggg aaatggctgc cgatgtcgcc gctgccgatc gcgcccagga
     1141 tcgcatcgac cagggcatgg atcgccacgt cggcatcgct gtgcccggcc agaccgcgtg
     1201 ggtgctcgat cttcactccg cacagccaca gctcctcgcc atcggccagg cggtgaacgt
     1261 catagccggt gccgatccgg accgccggag cggcggcgcc gaaatcgctg gcaaaggtca
     1321 gcttgtgcaa ggcttcgtct ccatcgacca gggccacgtc cagtccggcc gcccgcgcga
     1381 cctgggcatc gtcgccggca tctgccgcgc cggtccagcc gcggtgcgcg gcgaggattt
     1441 cggggtaacg gaacgcttgc ggggtctgca cccggcgcag ctcctcgcgc cgcgcgggtt
     1501 cgcccatccg cacgccctcg gcgcgggtca ggctgtccac caccggcagg acgggaatgg
     1561 cgcccgggtc cgcctccagg gcggccagca gccgctcgat caccgcctgc ggcaggaccg
     1621 ggcgcgcggc atcgtggatc agcacccgcg ccggagcatc gccttccagc gcctccagcg
     1681 ccagccggac cgattcctgc cgggtagccc cgccggtcac cagccgcacg ccgggaatgc
     1741 cgtccaccgc ggctgcggcc acgtctgccg cgccatccgg aatggctacg acgatcggcg
     1801 caacaccggc ggcggccagc gcctcggccg aatggcgcac caccggcttg ccccgccacg
     1861 gcgcgaactg cttgggcagc ggctgcccgg cgcgcaggcc ttgcccggcg gcaacgatca
     1921 cggcggcggt ggagcgggtt ggggcagggg ccatgcccat gccggtatag gcttgccgcg
     1981 cgcggagcaa ttccctatgt gctgcccaaa ttttaggcaa tcatccatga acccgcttcc
     2041 tccctctccc gggcttgccc cgatccaggt cggccccgtc ggcatcgact gcccggt
//
LOCUS       DASUDV010000450         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2069735,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000450 DASUDV010000000
VERSION     DASUDV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069735"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFV30_09765"
     CDS             complement(<1..760)
                     /locus_tag="VFV30_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX4848420.1"
                     /translation="MADTIFALSSGAPPAAIAVVRISGVRAGVALKALSGRLPPERRA
                     VAATLRDTAGAVLDRALVLWLPGPHTATGEDTAELHLHGGRAVVAAISAALAALPGLR
                     AAEPGEFTRRAFANGRIDLAEAEGLADLLSAETELQRQSALAMAGGAFSRQVEEWREG
                     LLGLSAAVEAVLDFADEDDVATLPADFLQRIDRLRGEIAVWLARPRAEVLREGFRVVI
                     AGPPNAGKSTLFNCLVQSDAAIATPIAGTTRDVLT"
     gene            complement(790..1047)
                     /locus_tag="VFV30_09770"
     CDS             complement(790..1047)
                     /locus_tag="VFV30_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HEX4848421.1"
                     /translation="MKINVEIDCTPEEARSFLGLPDVGKANQFYIEAISKAMKGAGNV
                     EQIQDIAKNIAPMGQMGLKLFQQFVENGAAFAAGAKKKSED"
     gene            1158..1856
                     /locus_tag="VFV30_09775"
     CDS             1158..1856
                     /locus_tag="VFV30_09775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4848422.1"
                     /translation="MATNTQFPSLDGAAQIPAYIARPTGTPKAAIIVIPEIFGLNPGI
                     QQKCETWASEGYLAIAPDIFWRFAPGVELDPDVPEQLQEAFGYFGQYDPDLGIQDIEA
                     AIHHIRRTEGVGKVGLVGYCLGGRMAYMAAARTDIDASVGYYGVMIDQMLGESHAIAN
                     PLMLHIPTADHFVGPEAQAAIHAGLDDHPKVTLHDYAGLDHGFAAEMGDRRDEAGAQL
                     ADSRTKAFLAANLA"
     gene            complement(1848..>2253)
                     /locus_tag="VFV30_09780"
     CDS             complement(1848..>2253)
                     /locus_tag="VFV30_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX4848423.1"
                     /translation="GIVEENARRCPATMAALELAPMPVSTGRSPNAHWSSLLPGTHIK
                     PHCGMLNTRLICHIPIQTAPNCWLRVGSDTRSWEPGVPLIFDDSIEHEAKNDGPQRRV
                     VLLFEIWRPEVPEEDRAAIARIFQAIGSYASG"
BASE COUNT          397 a          754 c          718 g          384 t
ORIGIN      
        1 gagtgaggac atcgcgcgtc gtcccggcga ttggcgtggc aattgccgca tcggactgaa
       61 ccaagcaatt gaaaagggtg ctttttccgg cgttcggggg accagcgatg accacccgga
      121 agccttcgcg caacacttcg gcgcgcgggc gggccagcca gaccgcgatc tcaccccgca
      181 ggcggtcgat ccgctggagg aaatcggccg gaagcgtcgc gacatcgtcc tcgtcagcga
      241 aatcgagcac ggcctcgacc gcggcggaga gaccgagcag gccttcgcgc cattcctcga
      301 cctggcgcga gaatgcgcca cccgccatgg ccagcgccga ctgccgctgc agctcggtct
      361 cggccgaaag cagatcggcc agcccttccg cctcggccag atcgatccgg ccattggcaa
      421 aggcgcggcg ggtgaactcg cccggttcgg cggcgcgaag gcccggcaag gctgccaatg
      481 cggcggaaat cgctgccacc accgcgcggc cgccatggag gtgcagttcg gccgtatcct
      541 cacccgtcgc ggtatggggt cctggtagcc acagtaccag cgcccggtcg agcacggcac
      601 ccgcagtgtc gcgcaaagtg gcggcaacgg cccggcgctc aggcggaagc cgcccggaca
      661 gggccttcaa cgccaccccg gcccgcaccc cgctgatccg gaccaccgca attgcggccg
      721 gcggcgcgcc gctcgacagg gcgaagatcg tgtcagccat ggtgcatcag acgatcccgg
      781 ttgagggact cagtcctcgc tcttcttctt ggcgccagcg gcaaaggccg cgccattctc
      841 gacgaattgc tggaacagct tcaggcccat ctggcccatc ggcgcgatgt tcttggcgat
      901 gtcctggatc tgctcgacat tgccggcgcc cttcatcgcc ttggaaatcg cctcgatata
      961 gaattggttg gccttgccga catcgggcaa gccaaggaac gaacgcgcct cttccggggt
     1021 acagtcgatc tcgacattga tcttcattat ggtctccttg cccgcgttcc tggttcccgc
     1081 gatcaagctg gcacaggccc ttggcaaagt ccatccagcc gacatattgg cagcaacccc
     1141 cagcagcgga gatttccatg gccaccaaca cccagttccc ctcgcttgac ggcgcggccc
     1201 agatcccggc ctatatcgcc cgtccgaccg gcacgccaaa agccgcgatc atcgtgatcc
     1261 cggagatctt cgggctgaat cccggcatcc agcagaagtg cgagacctgg gccagcgaag
     1321 gctatctggc gatcgccccc gatatcttct ggcgctttgc cccgggcgtg gagctcgatc
     1381 ccgatgtgcc ggagcagctg caggaagcct ttggctattt cgggcagtac gatcctgacc
     1441 ttggcattca ggatatcgag gccgcgatcc atcacatccg ccgcaccgaa ggcgtcggca
     1501 aggtcggcct agtcggctac tgcctgggtg gacggatggc ctatatggcg gcggcgcgga
     1561 ccgatatcga tgcctcggtc ggttactatg gcgtgatgat cgaccagatg ctgggtgaaa
     1621 gccatgccat cgccaatccg ctgatgctgc acatcccgac tgctgaccac ttcgtcgggc
     1681 cggaagccca ggcggcgatc catgccgggc tcgatgatca ccccaaggtg acgctgcatg
     1741 actacgcagg gctggaccac ggctttgccg ccgaaatggg tgaccgccgt gacgaggccg
     1801 gggcgcagct ggctgacagc cggaccaagg ccttcctcgc cgccaaccta gcctgaggcg
     1861 tagctgccga ttgcctggaa gatccgcgcg atcgccgcgc ggtcttcctc gggcacttcg
     1921 ggccgccaga tctcgaacag caggacgacg cggcgttgcg gcccgtcgtt cttcgcctcg
     1981 tgctcgatcg aatcgtcgaa gatcagcggc accccaggct cccagctgcg ggtatcgctg
     2041 ccaacgcgca gccagcagtt tggcgcagtc tggatcggaa tgtggcagat cagccgggtg
     2101 ttgagcatgc cgcagtgcgg cttgatatgg gtgccaggca gcaggctcga ccagtgggca
     2161 ttgggtgaac ggccggtact gaccggcatt ggcgccagtt cgagtgcggc catggtggcg
     2221 gggcaacggc gcgcattttc ctcaacgatg ccc
//
LOCUS       DASUDV010000451         6043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1451094,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000451 DASUDV010000000
VERSION     DASUDV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451094"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFV30_09785"
     CDS             <1..651
                     /locus_tag="VFV30_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HEX4848424.1"
                     /translation="GMQGTFSGGTAASLTETMKAVARNPSLIKILQSPFGLTPGFEGP
                     SQPLGLLPEYDESVGKWAAPFIMATINTKNVHRTNFLLGHPYGADFKYDEMMLTSAGE
                     IGEKAAHAVAEMLKNPFGAKPPKPGEGPSPEERENGFYDVLFIGEWPDGKTIRYGVKG
                     RYDPGYGSTSRMIAETGIALLDCKAEGGVSLPGALLGEALVQRLQDHAEITFAVED"
     gene            654..1025
                     /locus_tag="VFV30_09790"
     CDS             654..1025
                     /locus_tag="VFV30_09790"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HEX4848425.1"
                     /translation="MADNEQVVRDFIAAWSRLDVDELVSYFTPDGTYHNMPLAPVSGH
                     EALRTFIAGFAGGWASTAWETLHLVSSGNVVIAERVDRTVTRDGKTIDLPCCGVFELD
                     GGKIKVWRDYFDMGTYMKALA"
     gene            1044..2273
                     /locus_tag="VFV30_09795"
     CDS             1044..2273
                     /locus_tag="VFV30_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848426.1"
                     /translation="MTKLLSAVPDEFRNGWKIILGCSIGIASGVAILFLSFSLFVVPL
                     TTELQVSRGQLSNVQALIITAAIGSPLIGRLTDIYGVRRVYAASALLVAGTHLTSALF
                     ASNLIHMGLTIALVGLVGAGTGTVVLSRPINAAFKQYRGTALGLMAVGVSVLAILAPP
                     AINWVIENWGWRGAFAGMAAISLFAGIPAVLLLVPDTGRAGSGIGAASAPREPSDWRF
                     LRERDFWFLALALITMSLATAGAASQLAPMVQDSGVTAATAALALSFFSAGTFIGRLG
                     GGWLLDRMEPRRAAFLLTLLPAIGFVVLLTAGAVTPAIMLAVTIIGIQQGAEIDIFAY
                     MVGRRFDLAHYSAIYGALVGLGWIGNVGGLLGMGKAHDLFGSYAPAQAAAIGCLAVSA
                     LLLLAIRLPAAQESRAG"
     gene            2273..3475
                     /locus_tag="VFV30_09800"
     CDS             2273..3475
                     /locus_tag="VFV30_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848427.1"
                     /translation="MQPSEPGEWRTGWRIVAAMAVANGTGIALLFYTFSMFLIPISQE
                     LGLSRGEVGLVQAMIITAAIGAPLIGRIVDRVAFRTVFTVATLLLVAIELVQGRFVDS
                     LPLLALTVGLAGLIGGGASSVLLTRPVNAHFRRNRGLALGLVATGVSISTFLVPPVLH
                     QVIEGGGWRQGFYALALIAGGIGLPLVLLILPRNLPLNPVGAAASGIGAAPKPFLRQR
                     DFWLLVAANMLASISISGTISQLSPMIQDERISAGTAAFGLSLFAAGQFAGKLFGGWA
                     LDRFEPRRIAAAMTVLPATGFLIFLGDTGLTWPILLACTMIGVLQGAEVDIFAYFVAR
                     RFEVAQFGTVFGALHGFGWLGTALGIALFGFTFDLFGSYAPIQLGAVVLLGIVALLFL
                     PIRLPDES"
     gene            complement(3472..4101)
                     /locus_tag="VFV30_09805"
     CDS             complement(3472..4101)
                     /locus_tag="VFV30_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4848428.1"
                     /translation="MASTAPTLNPRAARTRAALLAAGFELLADRPIDAIAIDDIVARA
                     GVAKGSFFNHFTDKQGFAAAIGAAVRLELEARVGAANAGVDDPALRLAGGMRVAVAFA
                     LDERKRAIVMLRGLETSTGIDHELNQGIRADIDALVAAGLARPEAARSGVRYWLGLCQ
                     IAQLNAIERRPSRADAAERLREMLVLGLTGLGVPDARAQAVAAEVAARL"
     gene            4191..5210
                     /locus_tag="VFV30_09810"
     CDS             4191..5210
                     /locus_tag="VFV30_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4848429.1"
                     /translation="MSVIRIEDIAHVRYAAPDLGAMRAFLEDFGLSPFEAGGKLYGKG
                     SDGRPFVHVTEPGEAKFLAVGFRAETVTDLEKLAAHEGVPVEDLSEPGGGKLVRLTDP
                     DGYGVEVVAGQAKADPAPLPADQPVNTAAAKPRLRQTVRLDPAPAHVKRIGHAVLKVR
                     DFRVSEKWYKDRFGFLTSDEVEAAKDVPLGAFMRCDRGDKPADHHTLFLAQLPGELGI
                     LHAAFEVANLDDLMLGHQHLKTKKREAAWGVGRHIMGSQVFDYWKDPFGNELEHWTDG
                     DLFTAKDPPQKMPVSALLAVQWGAQHPMFAGKMPPPPGLVAFVMALQIRIKRLFSRSA
                     KEVSA"
     gene            5207..>6043
                     /locus_tag="VFV30_09815"
     CDS             5207..>6043
                     /locus_tag="VFV30_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4848430.1"
                     /translation="MKLCTYTAQGQTRTGIVVGDAVIDTGVPGTMIDLIRDWDSLKPQ
                     LEAKAAAGGGVPLASVKLEAPVQRPGKIFAIGLNYADHIAESNMGTPERQVWFTKAQT
                     SVNAPYAPIEIATGTMTADYEVELVAVVGKGGKHIAEDDAAGAIFGYCVGNDVTERMW
                     QHATPQWSLGKSFDTHAPMGPWIVTADELGDPHSLALRCFVNGEKRQDSNTRHLVFNI
                     WQQVEHLSTGMTLEPGDCLFTGTPGGIGAAMDPRQFLKAGDVVRCEVDGIGHIEGTMV
                     AEG"
BASE COUNT          927 a         2068 c         2064 g          984 t
ORIGIN      
        1 gggatgcagg gcaccttctc gggcgggacg gcggccagcc tgaccgaaac gatgaaggcg
       61 gtcgccagga acccctcgct gatcaagatc ctgcaaagcc cgttcggtct gaccccgggc
      121 ttcgaagggc cgagccagcc gctgggcctg ctgccggaat atgacgaaag cgtcggcaag
      181 tgggccgcgc cgttcatcat ggcgacgatc aacaccaaga acgtccaccg caccaacttc
      241 ctgctcggcc acccttacgg cgcggacttc aagtacgacg agatgatgct gaccagcgcg
      301 ggcgagattg gcgagaaggc cgcccacgcg gtcgccgaga tgctcaagaa cccgttcggg
      361 gccaagccgc ccaagccggg cgaggggccg agcccggaag agcgcgagaa cggcttttac
      421 gacgtgctgt tcatcggcga atggccggac ggcaagacca tccgctacgg cgtgaagggc
      481 cggtatgatc cgggctatgg ctcgaccagc cggatgatcg ccgaaaccgg cattgccctg
      541 cttgattgca aggccgaagg cggcgtttcg ctgcccggcg cgctgctcgg cgaagcgctg
      601 gtccagcggc tgcaggacca cgccgagatc accttcgcgg tcgaggacta ggcatggcgg
      661 acaacgaaca ggtggtgcgg gatttcatcg ccgcctggtc gcggctcgat gtggacgagc
      721 tggtttcgta cttcacgccc gatggcacct atcacaacat gccgctggcc ccggtcagcg
      781 ggcacgaggc gctccgtacg ttcatcgccg ggtttgccgg cggctgggcc agcaccgcgt
      841 gggagaccct gcacctggtt tccagcggca atgtggtgat tgccgagcgg gtcgaccgca
      901 cggtgaccag ggatggcaag accatcgacc tgccgtgctg cggggtgttc gaactggacg
      961 gcggcaagat caaggtctgg cgcgactact tcgacatggg cacctatatg aaggcgctgg
     1021 catgatggtg atggcctgac cgcatgacca aactgctgtc cgccgtgccg gacgaattcc
     1081 gcaacggatg gaagatcatc ctcggctgct cgatcgggat cgccagcggc gtggcgatcc
     1141 tgttcctcag cttttcgctg ttcgtcgtgc cgctgaccac ggagctgcag gtcagccgcg
     1201 gccagctgag caacgtccag gcgctgatca tcacggctgc gatcggctcg ccgctgatcg
     1261 gccggctgac cgacatctac ggggtccgca gggtctatgc cgccagcgcg ctgctggtcg
     1321 cgggcacgca cctgacctcg gccctgttcg catccaacct gatccacatg ggcctgacca
     1381 ttgccctggt cgggctggtc ggggccggga ccgggacggt cgtcctcagc cgcccgatca
     1441 acgccgcgtt caagcagtac cgcggcacgg cgctggggct gatggcggtg ggcgtatcgg
     1501 tgctggcgat cctggcgccg ccggcgatca actgggtgat cgagaactgg ggctggcgcg
     1561 gcgcctttgc cggcatggcg gcgatttcgc tgttcgcggg gatcccggcc gtgctgctgc
     1621 tggtgccgga caccggccgg gccggcagcg ggatcggcgc ggcttccgcc ccgcgcgagc
     1681 ccagcgactg gcggttcctg cgcgaacgcg atttctggtt cctggccctg gcgctgatca
     1741 cgatgagcct ggccacggcc ggcgcggcca gccagctggc gccgatggtg caggacagcg
     1801 gcgtcacggc cgccacggcc gccctcgccc tgtcgttctt ttccgccggc accttcatcg
     1861 ggcggctcgg cggcggctgg ctgctcgacc ggatggagcc gcgccgcgcg gccttcctgc
     1921 tgaccctgct gcccgcgatc ggctttgtcg tcctgctgac ggccggcgcg gtgacgccgg
     1981 cgatcatgct ggccgtgacg atcatcggca tccagcaagg ggccgagatc gacatcttcg
     2041 cctacatggt cgggcggcgg tttgacctgg cccattacag cgcgatctat ggcgcgctgg
     2101 tggggctggg ctggatcggc aatgtcggcg gcctgctcgg catgggcaag gcccatgacc
     2161 tgttcggcag ctatgccccg gcccaggccg cggcgatcgg ctgtctggcg gtgtcggcgc
     2221 tgctgctgct ggcgatccgc ctgcccgcgg cccaggaatc cagggccggg tgatgcaacc
     2281 gtccgaaccc ggcgagtggc gcaccggctg gcgcattgtt gccgccatgg ccgtggccaa
     2341 cggcaccggc attgcgctgc tgttctacac cttcagcatg ttcctgatcc cgattagcca
     2401 agaactcggc ctctcgcgcg gggaagtggg gctggtgcag gcgatgatca tcaccgccgc
     2461 gatcggcgcg ccgctgatcg ggcggattgt cgaccgggtg gccttccgca cggtctttac
     2521 cgtggccacg ctgctgctgg tggcgatcga gctggtccag ggccggtttg ttgacagcct
     2581 gccgctgctg gcgctgaccg tgggcctggc cgggctgatc ggcggcggcg cgtcctcggt
     2641 cctgctgacc cgcccggtca acgcccattt ccgccgcaac cgcgggctgg cgctgggtct
     2701 ggtggcgacc ggggtttcga tctccacctt cctcgtccca ccggtcctgc accaggtgat
     2761 cgagggcggc ggctggcgcc agggctttta cgcgctggcg ctgatcgctg gcggtatcgg
     2821 cctgccgctg gtcctgctga tcctgccccg caacctgccg ctcaacccgg tcggcgccgc
     2881 ggccagcggc atcggcgccg cgcccaaacc gttcctgcgg caacgcgatt tctggctgct
     2941 ggtggcggcc aatatgctcg cctcgatcag catttcgggc acgatcagcc agctttcgcc
     3001 gatgatccag gacgagcgga tctccgccgg aaccgccgcc tttggcctgt cgctgttcgc
     3061 cgccgggcaa ttcgccggaa agctgttcgg cggctgggcg ctggaccggt tcgaaccgcg
     3121 gcggatcgcg gcggcgatga ccgtgctgcc ggccaccggc tttctgatct ttctgggcga
     3181 taccggcctg acctggccaa tcctgctggc ctgcacgatg atcggggtgc tgcagggggc
     3241 cgaagtcgac atcttcgcct acttcgtcgc ccgccggttc gaagtggcgc agttcggcac
     3301 cgtgttcggg gcgctgcacg gctttggctg gctgggcacg gcgctgggca tcgcgctgtt
     3361 cggcttcacg ttcgatctgt tcggcagcta cgccccgatc cagctggggg cagtggtcct
     3421 gctggggatc gtggccctgc tgttcctgcc gatccggctg ccggacgaaa gctagagccg
     3481 cgccgccact tcggccgcca ccgcctgcgc gcgggcatcg ggcacgccca gcccggtcag
     3541 ccccaggacc agcatttcgc gcagccgctc ggccgcgtcg gcccggcttg gccggcgctc
     3601 gatcgcgttg agctgggcaa tctggcacag ccccagccaa tagcgcaccc cgctgcgggc
     3661 ggcttcgggg cgggccagtc cggccgccac cagcgcatcg atatcggccc ggatgccctg
     3721 gttgagctcg tggtcgatcc cggtcgacgt ctccagcccg cgcagcatca cgatcgcgcg
     3781 cttgcgctca tccagggcaa aggccaccgc cacccgcatg cccccggcca gccgcagggc
     3841 gggatcatca accccggcgt tggccgcgcc gacccgcgcc tccagctcca gccgcaccgc
     3901 cgccccgatc gccgcggcaa agccctgctt gtcggtgaag tggttgaaga agctgccctt
     3961 ggccacgccc gcgcgggcaa caatatcgtc gatcgcgatg gcatcgatcg ggcggtccgc
     4021 cagcagttcg aacccggcgg ccagcagcgc cgcgcgggtg cgggcggcgc gcggattgag
     4081 cgtgggagca gtgctggcca tgacccctta tcgccggact tgaccaaacg gtcaattcat
     4141 tctagttgac caatcagtca aaaacgaatc ggcggggaga ggacacgcgc atgagcgtta
     4201 tccgcatcga agacatcgcc cacgtgcgct atgccgcgcc ggaccttggc gccatgcgcg
     4261 ccttccttga agactttggc ctgagcccgt tcgaggccgg cggaaagctt tacggcaagg
     4321 gcagcgacgg acgccccttc gtccacgtga ccgagccggg cgaagcgaag ttcctggccg
     4381 tcggctttcg cgccgagacg gtcacggatc tggaaaagct ggccgcgcac gagggcgttc
     4441 cggtcgagga cctgagcgaa ccgggcggcg gcaagctggt gcgcctcacc gatcccgatg
     4501 gctacggcgt cgaggtcgtc gccggccagg ccaaggcgga ccccgccccg ctccctgccg
     4561 accagccggt caacaccgcc gccgccaagc cgcgccttcg ccagaccgtg cggctcgatc
     4621 cggctccggc ccacgtcaag cggatcggcc acgccgtgct caaggtccgc gacttccgcg
     4681 tgtcggaaaa gtggtacaag gaccgcttcg ggttcctgac ctcggacgag gttgaagcgg
     4741 ccaaggacgt gccgctgggc gccttcatgc gctgcgaccg gggtgacaag ccggccgacc
     4801 accacaccct gttcctggcc cagctgccgg gggaactggg cattctccac gccgccttcg
     4861 aagtcgccaa cctggatgac ctgatgctgg gccaccagca cctcaagacg aagaagcgcg
     4921 aggcggcctg gggtgtcggc cgccacatca tgggcagcca ggtgttcgat tactggaagg
     4981 acccgttcgg caacgagctg gaacactgga ccgacggcga tctgttcacc gccaaggacc
     5041 cgccgcagaa aatgccggtc agcgcgctgc tggcggtcca gtggggcgcc cagcatccga
     5101 tgtttgccgg caagatgccc ccgccgccgg gcctcgtcgc ctttgtcatg gcgctgcaga
     5161 tccgcatcaa gcgcctgttt tcccgttcag ccaaggaagt gtccgcatga aactctgcac
     5221 ctataccgcc cagggccaga cccgcaccgg gatcgtagtc ggcgatgccg tgatcgacac
     5281 cggcgtgccg ggcacgatga tcgacctgat ccgggactgg gacagcctga agccccagct
     5341 ggaggccaag gccgcggcgg gcggcggggt gccgctggcc tcggtcaagc tggaggcccc
     5401 ggttcagcgg cccggcaaga tcttcgcgat cggcctcaac tatgccgatc acatcgccga
     5461 atcgaacatg ggcacgcccg agcggcaggt gtggttcacc aaggcccaga ccagcgtcaa
     5521 cgcaccctac gctccgatcg aaatcgccac gggcaccatg accgccgact acgaggtcga
     5581 gctggtcgcg gtggtcggca agggcggcaa gcacattgcc gaggacgacg ccgccggtgc
     5641 gatctttggc tactgcgtgg gcaacgacgt gaccgagcgg atgtggcagc acgccacccc
     5701 gcaatggtcg ctgggcaaaa gctttgacac ccacgcgccg atgggcccgt ggatcgtgac
     5761 cgccgacgag ctgggcgatc cgcacagcct ggccctgcgc tgcttcgtca acggcgagaa
     5821 gcggcaggat tcgaacaccc ggcacctggt cttcaacatc tggcagcagg tcgaacacct
     5881 ctccaccggc atgaccctgg agccgggcga ctgcctgttc accggcaccc ccggcgggat
     5941 cggcgcggca atggacccgc gccagttcct gaaggccggc gatgtggtgc gctgcgaagt
     6001 ggacgggatc ggccacatcg aaggcaccat ggtggcggaa ggc
//
LOCUS       DASUDV010000452         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_59502,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000452 DASUDV010000000
VERSION     DASUDV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59502"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFV30_09820"
     CDS             complement(<1..776)
                     /locus_tag="VFV30_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4848431.1"
                     /translation="MSARFLYLDHQATTPLAPEARAAMEPWLAGPESMGFANPHSMHR
                     PGRAAAAAVEVAREQVAGLLPPGGRVIFTSGATEALNQAIRSVARGPIAYSAIEHSAV
                     VNPAIHTGAPFQELPVGADGLVDPEVEIAEGTGLVAVMQVNNEIGTVQPVARLAERAH
                     EMGALFLCDAVQGAGKLAPPEGADLIAVTAHKLYGPKGIGALWVRDGVKIGELIHGGG
                     QEGGLRSGTLSPALCAGFGAAAALAKERMEADFQHLSGLAA"
     gene            908..1564
                     /locus_tag="VFV30_09825"
     CDS             908..1564
                     /locus_tag="VFV30_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848432.1"
                     /translation="MPAVIFPGPEGRLEGRFQPATRSRAPVAMILHPHPQAGGTMNER
                     ITQHLYKTFAARGFATLRFNFRGVGRSQGSFDNGIGELSDAAAALDWVQSIHPEASTT
                     WVAGNSFGALIGMQLLMRRPEIRGFISVAPPANMYDFSFLAPCPASGIIIQGAADTVV
                     TPNSVQKLVDKLRTQKHITIHHEEIPRANHFFENEMEDLMRSVDNYLDMRLSPDCPIR
                     "
     gene            complement(1584..1787)
                     /locus_tag="VFV30_09830"
     CDS             complement(1584..1787)
                     /locus_tag="VFV30_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848433.1"
                     /translation="MALDTILSILMLAVIALVAGAIVLWRRGGRTRQVWLMLLLAAVA
                     AGNLAIWLVPDGSGKAPAEQVRQ"
     gene            complement(1793..>2013)
                     /locus_tag="VFV30_09835"
     CDS             complement(1793..>2013)
                     /locus_tag="VFV30_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848434.1"
                     /translation="VGGYLSSRAGGQGEGQGGGGMLGSILGAVLGGGQQAAPAGGGLL
                     GGLGSLIDMNKDGNPLDDIIGMAGKLAR"
BASE COUNT          326 a          768 c          626 g          293 t
ORIGIN      
        1 gcagccagcc ccgaaaggtg ctggaaatcg gcctccatcc gctccttcgc cagcgccgcc
       61 gccgccccga acccggcgca gagcgcgggg ctgagcgtgc cggagcgcag gcccccttcc
      121 tgcccgccgc cgtggatcag ctccccgatc ttcaccccgt cgcgcaccca cagcgcgccg
      181 attcccttgg ggccgtaaag cttgtgcgcg gtcaccgcga tcaggtccgc gccttcgggc
      241 ggggccagct tgcccgcgcc ctgcaccgca tcgcacagga acagcgcgcc catttcgtgc
      301 gcacgctcgg ccagccgggc aacgggctgg acggtgccga tctcgttgtt gacctgcatc
      361 accgcgacca ggcccgtgcc ctcggcaatc tccacctcgg ggtcgaccag cccgtccgcg
      421 ccgaccggca gttcctggaa cggcgcgccg gtgtggatcg cgggattgac cacggccgaa
      481 tgctcgatcg cggaataggc aattggcccg cgcgcaaccg agcggatcgc ctggttgagc
      541 gcctcggtcg cgccgctggt gaagatcacc cgcccgcccg ggggcaagag gcccgccacc
      601 tgctcgcgcg ccacctccac ggcggcggcc gctgcccggc ccggccggtg catcgagtgc
      661 gggttggcaa agcccatcga ttccggtccg gccagccacg gctccatcgc cgcgcgcgct
      721 tcgggggcaa gcggcgtggt ggcctggtga tcgagataga ggaatcgggc cgacatgttc
      781 gcaatcgttg ctttttggac actcgtttct atatagcgct cgcttcccga tcctagccca
      841 aagcggaccg gcatccgaga aatccccgga cgccgcgcct ggggccagca agacaggccg
      901 tgataccatg cccgcagtga tctttcccgg acccgaaggc cgccttgaag gccgtttcca
      961 gcccgccacc cgcagccgcg cgccggttgc catgatcctg cacccgcacc cccaggcggg
     1021 cggcacgatg aacgagcgga tcacccagca cctctacaag acctttgccg cgcgcgggtt
     1081 cgccacgctg cgcttcaact tccgcggggt tggccgcagc cagggcagct ttgacaacgg
     1141 gatcggcgaa ctgtcggacg ccgccgccgc gcttgactgg gtccagtcga tccaccccga
     1201 agccagcacc acctgggtcg ccggcaacag ctttggcgcg ctgatcggga tgcagctgct
     1261 gatgcgccgc ccggaaatcc gcggcttcat ctcggtcgcc ccgccggcca acatgtacga
     1321 tttcagcttc ctggcgccct gcccggcctc gggcatcatc attcagggcg cggccgatac
     1381 ggtggtgacg cccaactcgg tgcaaaagct ggtcgacaag ctgcgcacgc aaaagcacat
     1441 cacgatccat cacgaggaaa tcccccgggc gaaccatttc ttcgagaacg agatggaaga
     1501 cctgatgcgc tcggtcgaca actacctcga catgcggctc tcgccggact gcccgattcg
     1561 ctgatcgggc agccggctga aggctattgc cgaacctgtt cggccggggc cttgccagag
     1621 ccatcgggca ccagccagat cgccagattg ccggccgcca ccgcggccag cagcaacatc
     1681 agccacacct gacgggtgcg ccccccgcgc cgccacagca cgatcgcgcc cgccaccagc
     1741 gcgatgaccg ccagcatcag gatcgagagg atcgtgtcga gcgccacggc gtctaccgag
     1801 ccagcttgcc cgccatgccg atgatatcgt cgagcgggtt gccgtccttg ttcatgtcga
     1861 tcagcgagcc gagcccgccc agcagcccgc caccggccgg cgccgcctgc tgaccgccgc
     1921 ccagcaccgc gccgaggatc gagccgagca tgccgccccc gccctggcct tcgccctggc
     1981 cgccggcccg cgaggacagg tatccgccga cca
//
LOCUS       DASUDV010000453         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1870805,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000453 DASUDV010000000
VERSION     DASUDV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870805"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VFV30_09840"
     CDS             <1..1102
                     /locus_tag="VFV30_09840"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX4848435.1"
                     /translation="ATNALASALLRDGEAERAIALLEPQVRRFAQPCPERSVALSLLG
                     DANGRLGRTDAAYAAYAASKADFAAINTRPGMGEIGNREFVEAIRDAFSASPAEAWRQ
                     APGGQPHNACSPHVFLLGFPRSGTTLVENVLATLPGVAALEERPTLIEADSAYLLGDR
                     AAVAAGLARFAALDEAGLARLRAAYWNKVAASGVSADATAFVDMDPLKGSRLPLIARL
                     FPEARILIMRRDPRDVVWSCFRTNFAMSSGTLEFTTLEKAARHYDAMMQLIELARERL
                     NLAMLEVHYHRLVQDFDATTREICSFIGLDWSEDLRRFDRTAQRRGVATASAGQVRRG
                     LYDGTRQWEPYARHLEPVMPLLQPWIERFGYA"
     gene            complement(1093..>2023)
                     /locus_tag="VFV30_09845"
     CDS             complement(1093..>2023)
                     /locus_tag="VFV30_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848436.1"
                     /translation="ADHAGAAFLTDDYLGPREVGGDVAAEQCLALPGDAPLGLIFHSA
                     FCGSTMLLRALDRPGVAMGLSEPVVLNDVVGFRRRGADPRAVARAADAATRLLARPFG
                     PGEAVVIKPSNVVNPLAELLLMLRPKAQAIFLHAPLETFLISVVRKGLPCRLWVRELL
                     EGYLRENFVQLGFEPQDYFRQSDLQVAAVGWLAQHMHFARLAAKLGPERLRSLDADRM
                     TADPAAAIAASAMHFGLSLTPEVLGEIVSGPAFTRHSKSGAAFNAETRTSEYAAARAA
                     HGEEIDLVLRWAAQVAERAGIAMQAPAPLLAQA"
BASE COUNT          342 a          715 c          651 g          315 t
ORIGIN      
        1 ggctaccaac gcgctggcca gtgccctgct gcgtgatggc gaggccgaac gcgcgatcgc
       61 attgcttgaa ccgcaggtgc ggcgctttgc ccagccctgt cccgaacgct cggtcgcgct
      121 cagcctgctc ggcgatgcca acggccggct gggacggacc gacgccgcct atgccgccta
      181 cgccgccagc aaggccgatt tcgccgcgat caataccaga ccgggcatgg gcgagatcgg
      241 caaccgcgaa ttcgtggaag cgatccgcga tgcattttcc gccagtccgg ctgaagcctg
      301 gcggcaggca cccggcgggc aaccccacaa tgcctgctcg ccccacgtgt tcctgctggg
      361 attcccccgt tcaggcacca cgctggtcga aaacgtcctc gccacgcttc ccggcgttgc
      421 cgcgctcgag gaacgcccga ccctgatcga ggccgactcg gcctatctcc tcggcgatcg
      481 cgccgcagtt gccgccgggc tcgcccgctt tgccgcgctc gacgaggcag ggcttgcccg
      541 gctgcgcgcc gcctattgga acaaggttgc cgcatccggc gtttccgccg acgccacggc
      601 ctttgtcgat atggatccgc tcaagggatc acgcctgccg ctgatcgcgc ggctgttccc
      661 agaggcgcgg atcctgatca tgcggcgcga tccgcgcgac gtggtgtgga gctgctttcg
      721 caccaatttc gcgatgagca gcgggacgct ggaattcacc acgctcgaaa aggccgcgcg
      781 tcattacgat gcgatgatgc aactgatcga actggcgcgc gaacggctga accttgccat
      841 gctcgaggtt cactatcacc gtctggtcca ggatttcgat gccacaaccc gcgaaatctg
      901 cagcttcatc ggccttgact ggagcgagga cctgcgccgg ttcgatcgca ccgctcagcg
      961 ccgcggggtg gcaacggcca gcgcgggcca ggtgcggcgc gggctttacg acggcacccg
     1021 tcaatgggag ccctatgccc gtcacctcga gccggtcatg ccgctgctgc agccctggat
     1081 cgagcgcttc ggctacgcct gagcaagaag cggtgcgggc gcctgcatcg cgatccccgc
     1141 gcgttcagcc acctgtgcgg cccagcgcag caccagatcg atctcctcgc catgcgcggc
     1201 gcgggcggcg gcatattcac tcgttcgggt ttccgcatta aacgccgcgc ccgatttcga
     1261 atggcgggtg aaggccgggc cgctcacgat ctcaccgagc acttcggggg tcagcgacag
     1321 gccgaaatgc atggcgcttg cggcaatggc tgcggccgga tcggcagtca tccgatcggc
     1381 atcgagactg cgcagccgct ccggccccag cttggcggcg aggcgggcaa aatgcatgtg
     1441 ctgcgccagc cagccgaccg cggccacctg caggtcgctc tggcggaaat agtcctgggg
     1501 ttcgaagccg agctggacga aattctcgcg cagatagcct tccagcagct cgcgcaccca
     1561 cagccggcac ggcaagccct tgcgcacgac cgagatcagg aaggtctcga gcggggcatg
     1621 gagaaatatc gcctgcgcct tcgggcgcag catcagcagc agttcggcca gcggattgac
     1681 gacgttcgaa ggcttgatca ccaccgcttc gcccggtccg aacggccgcg ccagaagccg
     1741 cgttgcggca tcggcggcgc gggccaccgc acgcggatcg gcgccgcgcc gccggaagcc
     1801 gaccacatcg ttgagcacga ccggttcgga cagccccatc gccactccgg gccgatcgag
     1861 cgcgcgcagc agcatggtcg atccgcagaa tgccgaatgg aagatcagcc ccagcggagc
     1921 atcgccgggc agcgccaggc attgctccgc cgccacatcg ccgcccactt cgcgcggacc
     1981 cagatagtca tcggtcagga acgccgcgcc agcatgatcc gcg
//
LOCUS       DASUDV010000454         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2157966,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000454 DASUDV010000000
VERSION     DASUDV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157966"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFV30_09850"
     CDS             <1..576
                     /locus_tag="VFV30_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4848437.1"
                     /translation="RGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRT
                     SRQFLHEQGREPTPEEMAERLSMPLEKVRKVMKIAKEPISLETPIGDEEDSHLGDFIE
                     DKNAVIPVDAAIQANLKDTVTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSV
                     TRERIRQIEAKALRKLKHPSRSRKMRSFLDQ"
     gene            653..1267
                     /locus_tag="VFV30_09855"
     CDS             653..1267
                     /locus_tag="VFV30_09855"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX4848438.1"
                     /translation="MIPYLSERGPEFMTWRFALAALALVPTPALADVIPADFACIDKT
                     TDPAKFELARGIVNASVPDSQQEKMFDDMLDSLIGMLRSNRDPALVDPEMDAIVDRQL
                     KSIPSRLVPLLNRHMPQMYGAIACAYAREFSLAELKDINAFALSPSGRRFLTHSSVLM
                     ADPDVQKAFQAYMQDVQKLTREMTDEMMAELAELRAKRAKKSKN"
     gene            complement(1280..2950)
                     /locus_tag="VFV30_09860"
     CDS             complement(1280..2950)
                     /locus_tag="VFV30_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4848439.1"
                     /translation="MRTFVLALMGAVVLAILATTPPGPRGSAAPADAFSASRAMEHVR
                     RIADRPHVTGSAENAAVRGYIAERMQALGMEVTTRDAPLSDRGRERLIRWSGMPPEAA
                     PRTLTNVIGILPGRDRSLPAVMLMAHHDTVWGSPGASDDTAGVAASLEVVRAIAAKGP
                     PPRDLIVLVTDAEELGLEGAEHFFAADPLRSRVGAIINMEARGGGGRTSLFQTSRGNG
                     AAVQLYAGAVSRPAASSLAAYVYSVLPNDTDLTPAIAGPYTAYNFAFIGRPGLYHSPL
                     ATPDRLDQGSLQDMGGQVLDLTGALLSVPILPDKAPDAVFFDLFGLTTVIFPVWLGWL
                     MLVQAGLLLAMVIRRDGRGGLVEGAARMLGLLLIGGGLLYLFNQLSLGFARGEYYDRL
                     AAIPRLEGMALLIGGAVFLAMFGARKGTPVLTAGAALPLWLLALVAQVLAPTAAYVLT
                     LPLLLVGAVLWRPGAVQAGATAAVVTGYMLALGHQLMQGVGPTLPMAAALPLALGVLA
                     LLPVWPGIEKRRAGILAALLLTSAAAVALWVQLDAPAETRAVYADSKR"
     gene            3125..3562
                     /gene="rpiB"
                     /locus_tag="VFV30_09865"
     CDS             3125..3562
                     /gene="rpiB"
                     /locus_tag="VFV30_09865"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX4848440.1"
                     /translation="MRIAIASDHAAVDLKAELRDYLIALGHEVADLGPDTADRVDYPD
                     YGYKLASVVAEGLVQYGVALCGSGIGISIAVNREPACRCALVSEPLSAALAREHNDAN
                     VIAMGARLTGVDMAKACLDAFLSTDFGGGRHAGRVEKLSNPSC"
BASE COUNT          650 a         1240 c         1145 g          534 t
ORIGIN      
        1 cgcggctaca agttcagcac ctatgccacc tggtggatcc ggcaggcgat cacccgctcg
       61 atcgccgacc aggcccgcac gatccgcatc ccggtccaca tgatcgagac gatcaacaag
      121 ctggtccgca ccagccgtca gttcctgcac gagcagggcc gtgagccgac gcccgaggaa
      181 atggccgaac gtctgtcgat gccgctcgaa aaggtccgca aggtgatgaa gatcgccaag
      241 gagccgatct ccctcgaaac cccgatcggg gacgaggaag acagccacct gggcgatttc
      301 atcgaggaca agaacgcggt gatcccggtc gacgcggcga tccaggccaa cctcaaggac
      361 acggttaccc gggtcctggc cagcctgacc ccgcgcgagg aacgcgtgct gcgcatgcgt
      421 ttcggcatcg gcatgaacac cgatcacacg ctggaagaag tcggccagca gttctcggtc
      481 acccgcgaac gtatccgcca gatcgaagcg aaggcgctga ggaaactcaa gcacccgagc
      541 cggtcaagga agatgcgaag cttcctggat cagtaatccc aacaagaaga agcgggcatc
      601 gattggcccg cttctttttt ggccgctgtt gacggggcgg ttccgatccg tgatgatccc
      661 ctatctttct gaaaggggac cggaattcat gacctggcgt ttcgcattgg ccgctctggc
      721 gcttgttccg accccggctc tggccgacgt tattccggcg gatttcgcct gcatcgacaa
      781 gaccaccgat cccgccaagt tcgaattggc gcgtggtatc gtcaacgcct cggttccgga
      841 cagccagcag gaaaaaatgt tcgatgacat gctggactcg ctcatcggga tgctccgaag
      901 caaccgcgac cctgcgcttg ttgatcccga aatggacgcg atcgtagatc gtcagctcaa
      961 gagtattccg agccgactgg tgccgcttct gaaccgccac atgccgcaaa tgtacggcgc
     1021 aatcgcgtgt gcctatgccc gcgagttctc ccttgcggaa ttgaaggaca taaacgcctt
     1081 cgccctgtca ccgtccggcc ggcgctttct gacgcattct tcggtgctga tggccgatcc
     1141 ggatgtccag aaggccttcc aggcctacat gcaggacgtg caaaagctga cccgcgagat
     1201 gaccgacgag atgatggccg agttggcgga gcttcgcgcg aaacgcgcca agaagtccaa
     1261 gaactgagcg gtctgcccgt cagcgcttgc tgtcggcata gaccgcgcgg gtttccgcgg
     1321 gcgcgtcaag ctgcacccac agcgccacgg ccgcggcact ggtgagcagc aaggcggcca
     1381 ggatcccggc gcggcgcttc tcgatccccg gccagaccgg cagcagagcc agcacgccca
     1441 gcgccagcgg caatgccgca gccattggca gggtcggccc caccccttgc atcagctggt
     1501 gccccagcgc gagcatgtag ccggtgacca cggccgcggt cgctcccgcc tgaacagcgc
     1561 cagggcgcca cagcacggca ccgaccagca gcagcggcag ggtcagcaca taggccgctg
     1621 tcggcgccag cacttgggcc acaagggcca gcagccacag cggcaatgcc gcgcccgcag
     1681 tgaggaccgg agtccccttc cgcgcgccga acattgcgag gaacaccgcg ccgccgatca
     1741 gcagcgccat gccctccagc cgggggatcg ccgccagccg gtcatagtat tcgccccgcg
     1801 caaagccgag cgagagctga ttgaacaggt agagcagccc gccgccaatc agcagcaggc
     1861 cgagcatccg cgcggcccct tcgaccagcc cgcctcgccc gtcccgccgg atgaccatag
     1921 ccagcagcag gcccgcctgc acaagcatca gccaaccgag ccagaccggg aagatcacgg
     1981 tggtcagccc gaacaggtca aagaacacag catcgggtgc cttgtccggc aggatcggta
     2041 ccgacagcag cgccccggtc agatccagca cctggccgcc catgtcctgc agcgagccct
     2101 ggtcgagccg gtcgggcgtg gccagcggcg aatggtacag gcccgggcgg ccgatgaagg
     2161 cgaagttgta ggccgtatag ggtccggcaa tcgccggggt caggtcggta tcgttgggca
     2221 ggaccgaata gacataggcc gccagcgagc ttgccgccgg gcgcgacacg gccccggcat
     2281 agagctgcac ggcagcgccg ttcccgcgcg aggtctggaa caggctggtc cgcccgcctc
     2341 cgccgcgcgc ctccatgttg attatcgcgc cgacacggct gcgcagcgga tcggcggcga
     2401 agaaatgctc ggccccttcc agccccagtt cctcggcatc ggtcaccagc acgatcaggt
     2461 cgcgcggcgg cggccccttg gccgcaatcg cccggaccac ttccaggctg gcggccaccc
     2521 cggcggtgtc gtcggacgcg ccgggcgagc cccagaccgt atcgtggtgc gccatcagca
     2581 tcaccgccgg caggctgcga tcccgcccgg gcaggatccc gatcacgttg gtcagggttc
     2641 ggggagcggc ttcgggcggc atcccgctcc agcggatcag gcgctcgcgg ccgcggtcgc
     2701 tcagcggagc gtcccgggtg gtcacttcca tgccaagggc ttgcatccgc tccgcgatat
     2761 agccgcgcac cgcggcgttc tcggccgatc cggtcacgtg cgggcgatcg gcaatccgcc
     2821 ggacatgctc catggcccgg ctggccgaaa aggcatcggc cggggcagcg ctgccgcgcg
     2881 gaccgggcgg cgtggtcgcc aggatcgcca gcaccaccgc gcccatcagc gccagcacga
     2941 atgtccgcat cgcatccccc ttgtccgcgg cgatgctggg gacggcctgc ccccttgtca
     3001 atccgcggcc ccggcggccc aagatcacca gaatttacgg taggtatgct ggcaaagcgg
     3061 gtggccgccg actaaggggt ggcgcgagcg attctcacct ttcgttgcgg ggccaggcca
     3121 acccatgcgc attgccattg cttccgatca cgccgccgtc gacctgaagg cggagctgcg
     3181 cgactatctg atcgcgctgg gccacgaagt ggcggacctg gggccggaca cggccgaccg
     3241 ggtcgactac cccgattacg gctacaagct cgcctcggtc gtggccgaag ggctggtcca
     3301 gtacggcgtg gcgctgtgcg gcagcgggat cggcatctcg atcgcggtca accgcgaacc
     3361 ggcctgccgc tgcgcgctgg tgtccgagcc gctgtccgcc gccctggccc gcgaacacaa
     3421 cgatgccaac gtgatcgcca tgggcgcccg gctgaccggg gtggacatgg ccaaggcctg
     3481 tctcgacgcg ttcctttcca ccgatttcgg cggtggccgc catgccggcc gggtcgaaaa
     3541 gctctccaat cccagctgct gaggagccc
//
LOCUS       DASUDV010000455         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_611958,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000455 DASUDV010000000
VERSION     DASUDV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611958"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2151
                     /locus_tag="VFV30_09870"
     CDS             <1..2151
                     /locus_tag="VFV30_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX4848441.1"
                     /translation="AVVDYVSKLGSGDRAKGGSTITQQVAKNILIGNEYSVTRKLKEM
                     VLARRIEGVLSKEQILELYLNEIPLGRQSFGVEAAAQAYFGKSVSEITLAEAAFLAAL
                     PQGPEIYGRERHAARALERRNWVLDQMVKNGAVSAAEAAQAKAQPLGIIPRRSEVYDP
                     SSGYFVEEVRRTLIEKYGESAEDGPNSVYSGGLWVRTSLDPQLQKATQDALRGGLMRF
                     GGARAFDKPLETVDLDPDKWQSQLIATGKSINYQDWRIAIVIERGSAKTTVGFADGKT
                     GTLVSPPAALKEGHVIAVAPAGNAWALRVVPTISGGMLLQEPFSGRVLAMQGGFDSGL
                     ASFNRATQAERQPGSTIKPFVYGTGLDFGMSPSSEVVDGKFCVYQGARFGQKCFQNFG
                     GGAGGGTHTMRWGLEQSRNLMTVRIANDAGMDRVANTIERVGIGKYPPYLAFALGAGE
                     TTVLKMVNAYSALANNGIQYPPSLIDYIQDRSGKVIWRADTRRCDKCNMAEWDGKPMP
                     RLPARGKEVMDPRTAFQVVHMLEGVVTRGTAITLADLGLPLFGKTGTTTGPTNVWFVG
                     GNQEIVGGVYLGYDQPASLGGYAQGGTIAAPIFKQMVQATKPRWSRRPFTAPKGIHWV
                     RVDRISGKRVLDGMPGSDPKSSIIWEAFKAESEARTGPSTEALAAQRDALLKSLRRGA
                     TRKDQPAGAEGDPGLATEPKAAPTIAADPGVAGQ"
BASE COUNT          385 a          717 c          753 g          313 t
ORIGIN      
        1 gcggtggtgg actacgtgtc caagctcggc tcgggcgacc gggccaaggg cgggtcgacc
       61 atcacccagc aggtcgccaa gaacatcctg atcgggaacg agtactctgt cacccgcaag
      121 ctcaaggaaa tggtgttggc gcgccggatc gaaggggtgc tgagcaagga gcagatcctt
      181 gagctctacc tcaacgaaat cccgctcggc cggcagagct tcggggtcga agccgccgcc
      241 caggcctatt tcggcaagag cgtgagcgag attaccctgg ccgaggcggc tttcctcgcg
      301 gccctgccgc aggggcccga aatctatggc cgcgaacggc acgccgcccg cgcgctggag
      361 cgccgcaact gggtgctgga ccagatggtc aagaacggcg cggtttccgc ggccgaggcg
      421 gcccaggcca aggcccagcc gctcggcatc attccgcgcc ggtccgaagt ctacgatccc
      481 tcgtcgggct acttcgtcga ggaggtgcgc cgcaccctga tcgagaagta cggcgaaagc
      541 gccgaggacg ggcccaacag cgtctattcg ggcggcctgt gggtccgcac ctcgctcgat
      601 ccgcagctgc agaaggccac ccaggacgcg ctgcgcgggg ggctgatgcg gtttggcggg
      661 gccagggcct tcgacaagcc gctggagacc gtcgatctcg atcccgacaa gtggcagagc
      721 cagctgatcg cgaccggcaa gtcgatcaat taccaggact ggcggatcgc catcgtgatc
      781 gagcgcggca gcgccaagac cacggtcggg tttgccgacg gcaagaccgg cacgctcgtc
      841 tcgccgcccg cggcgctcaa ggaagggcac gtgatcgcgg tggccccggc cggaaacgcc
      901 tgggcgctgc gggtggtgcc gacgatttcg ggcgggatgc tgctgcagga accgttctcg
      961 ggccgggtgc tggcgatgca gggcgggttc gattcgggcc tggcgagctt caaccgcgct
     1021 acccaggccg aacgccaacc cggctccacg atcaagccgt tcgtctatgg caccggcctt
     1081 gatttcggga tgagcccgtc gagcgaagtc gtcgatggca agttctgcgt ctatcagggc
     1141 gcgcggttcg ggcagaagtg tttccagaac tttggcggcg gggccggcgg cggcacccac
     1201 acgatgcgct ggggccttga acagtcgcgc aacctgatga ccgtgcggat cgccaacgac
     1261 gccgggatgg accgggtggc gaacacgatc gagcgcgtcg gcatcggcaa gtatccgccc
     1321 tatctggcct tcgcgctggg cgctggggaa accaccgtgc tcaagatggt caacgcctat
     1381 tccgcgctgg ccaacaacgg gatccagtat ccgcccagcc tgatcgacta tatccaggac
     1441 cgctcgggca aggtgatctg gcgggccgac acccgccgct gcgacaagtg caacatggcc
     1501 gagtgggacg gcaagccgat gccccgcctg ccggcccgcg gcaaggaagt gatggacccg
     1561 cgcaccgcgt tccaggtggt ccacatgctg gaaggcgtgg tcacccgcgg caccgccatc
     1621 acgctggccg atctgggcct gccgctgttc ggcaagaccg gaaccaccac gggcccgacc
     1681 aacgtgtggt tcgtgggcgg caaccaggaa atcgtcggcg gggtctatct cggctacgat
     1741 cagcccgcgt cgctgggcgg ctatgcccag ggcgggacga ttgccgcgcc gatcttcaag
     1801 cagatggtcc aggccaccaa accgcgctgg agccgccggc cctttaccgc gcccaagggg
     1861 atccattggg tccgggtgga ccggatcagt ggcaagcggg tgctggacgg aatgccgggc
     1921 agcgatccca agtcctcgat catctgggaa gccttcaagg ccgaatccga agcccgcacc
     1981 gggcccagca ccgaagctct ggcggcgcag cgcgatgccc tgctcaaatc actgcggcgc
     2041 ggcgccacgc gcaaggatca gcccgccggg gccgaaggcg atccgggcct ggcgaccgaa
     2101 cccaaggccg caccgaccat tgcggctgat cccggggtgg ccgggcaata gccacgctgg
     2161 acagcgcg
//
LOCUS       DASUDV010000456         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1384758,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000456 DASUDV010000000
VERSION     DASUDV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384758"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFV30_09875"
     CDS             complement(<1..275)
                     /locus_tag="VFV30_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848442.1"
                     /translation="MTASKTMDHGSRFFAVSAMLIAVLVVLSFPLTYYIPLLTGTKSF
                     TLLRHLHGLAFFAWIGLYLAQTQLVRHGKVKLHRELGIAGVALAGAMV"
     gene            410..1138
                     /locus_tag="VFV30_09880"
     CDS             410..1138
                     /locus_tag="VFV30_09880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848443.1"
                     /translation="MSALYDTIGIDYADLRRPDPRIAALIEAALGNARSVLNVGAGTG
                     SYEPAGRDVTALEPSAEMIAKRPAGAAPAVQGGAEDLPFADASFDAVMAVLTVHHWSD
                     QARGLSELRRVSRGRVVILTFDPVARPWLTDYLPQLAALDEAAMPAIDSYERVLGPVE
                     ISPVPVPGDCSDGFLYAYWRRPRAYLDPRIRSGSSSFWLLDEVEAGLERLRADLESGE
                     WERRYGALLDSDYYDAGYRLIVAG"
     gene            1162..2256
                     /locus_tag="VFV30_09885"
     CDS             1162..2256
                     /locus_tag="VFV30_09885"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4848444.1"
                     /translation="MRAAFWKALCALWLISLGLAAPLRADAIEDAINAELRRQDIPGL
                     AVAVVERGRVVRLSGHGLANLEHRVPVTPDTVFQGGSTAKQFTALGILLLAADGKLAL
                     DDPLSRYLPDVPRRWRDISLRHLMVQTSGLGEDPPRADYQRSPAQADLRRLFYTTPKT
                     RPAGAAWAYSNTGYAMLGMVIEKVTGQPYHRFLEQRLFRPLGMTATTGINEAAIIANR
                     AAGYEREGGKLGAPLRNQAWVSQVFNSTADGSTYITARDFATFLAALDNPPAQFAPHF
                     AQIVKPVAPIAKDSAVSYGFGWFVTRIDGREVLFHSGSWQGFRVHMIRYPQQQTSVVM
                     LANSDIPERAALVRAVLGAVLPGMPAPPGW"
BASE COUNT          395 a          758 c          776 g          438 t
ORIGIN      
        1 accatcgctc cggccagggc gaccccggca atccccagtt cgcgatgcag cttcaccttg
       61 ccgtgccgca ccagctgtgt ctgcgccagg tagagcccga tccaggcaaa gaaggccagg
      121 ccatggagat ggcgcagcaa agtgaagctt ttggtcccgg tcaggagcgg gatatagtag
      181 gtcagcggaa agctgagcac gaccagaacc gctatcagca tggcgcttac cgcaaagaag
      241 cggctgccat ggtccatcgt cttgctggcc gtcatcgcgt ccccccgttc gcgtccaggt
      301 aaaaggctaa caccgttctc gctgcgagcg aaatgatgaa acgccttgat cgccgctgcg
      361 acgcgcaggt gtatgcaatc gctcgcgaag attgtaggca ggtagaaccg tgagcgcgct
      421 ctacgacaca atcggcatcg attatgctga cctgcgccgg cccgatccgc gcatcgccgc
      481 gttgatcgag gctgcgctgg gcaatgcccg ctctgtgctg aacgtcggcg ctggcacagg
      541 ctcctacgag cccgcggggc gggatgtcac cgcgctggag ccttcggccg aaatgattgc
      601 caaacgccct gccggcgccg ccccggcggt acaggggggt gcggaagacc tgccgttcgc
      661 cgatgccagc ttcgatgcgg tgatggcggt gctgacggtg catcactggt ccgatcaggc
      721 cagggggctg tccgaactgc gccgggtcag ccggggccgg gtggtgatcc tgaccttcga
      781 tcctgtcgcc cggccctggc tgaccgatta cctgccgcag ctggcggcgc ttgacgaagc
      841 ggcgatgccg gcgatcgaca gctacgaacg tgttctggga ccggtcgaga tctctccggt
      901 tccggttccc ggcgattgca gcgatggctt cctctatgcc tactggcggc ggccccgggc
      961 ctatctcgac ccacgaatcc ggtcgggcag ttcctcgttc tggctgctgg acgaagtcga
     1021 agccgggctg gaacgcctgc gcgccgatct ggaatcgggc gagtgggagc ggcgctatgg
     1081 cgccttgctg gattcggatt actatgacgc gggataccgg ctgatcgtgg ctggatagtc
     1141 gcaaaatcgg ggggcaaggc gatgcgggca gcattctgga aagcgctttg cgcactctgg
     1201 ctgatcagcc ttggtctggc cgcaccgctc cgtgctgatg cgatcgagga tgcaatcaat
     1261 gccgaactgc gccggcagga catccccggg cttgccgtgg cggtagtcga acggggcagg
     1321 gtggtgcggc tttccggcca tggtctggcc aatcttgaac atcgcgtgcc ggttaccccc
     1381 gatacggtgt tccagggcgg gtccaccgcc aagcaattca ccgcgctggg aatcctgctg
     1441 ctggcggccg atggcaagct ggcgctggac gatccgctgt cgcgctatct gccggatgtg
     1501 ccccgccgct ggcgggatat cagcctgcgc catctgatgg tccagacatc gggcctgggc
     1561 gaggatccac ccagggcgga ttatcagcgc agcccggccc aggcagacct gcgccgcctg
     1621 ttctacacca cccccaagac ccggcccgca ggcgccgcct gggcctattc caacacgggc
     1681 tatgccatgc ttggcatggt gatcgagaag gtcaccggcc agccctatca tcgcttcctt
     1741 gaacagcggc tgttccgccc gctgggcatg acggcgacca cgggtatcaa cgaggcggcg
     1801 atcatcgcca accgggctgc cggttacgaa cgcgagggcg gcaagctggg cgcgccgctg
     1861 cgcaaccagg cctgggtttc gcaggtattc aacagcaccg ctgatggcag cacctatatc
     1921 accgcgcgcg acttcgcgac tttcctggcg gcgctcgaca atcccccggc acagttcgct
     1981 ccgcacttcg cgcagatcgt caagccggtt gcgccaattg cgaaggactc ggcggtttcc
     2041 tatggctttg gctggttcgt cacccggatc gacggacgag aggtgctgtt tcattccggc
     2101 tcgtggcagg gcttccgggt tcatatgatc cgctatcccc aacaacagac cagtgtggtg
     2161 atgctggcca attccgacat tcccgaacgt gccgccctgg tcagggccgt gctgggcgcg
     2221 gtgctgccgg gcatgccggc tccgcccggc tggtaaggcg gggcatgtcc tgcggcagtg
     2281 attgccctgc cggcaacaaa cgcagagcct tggtctgcgc ggtctagcgc ttttccagcc
     2341 aggccttggt ttccgagatg aattcca
//
LOCUS       DASUDV010000457         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_81767,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000457 DASUDV010000000
VERSION     DASUDV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81767"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFV30_09890"
     CDS             complement(<1..1223)
                     /locus_tag="VFV30_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4848445.1"
                     /translation="MSEAVAAAWTNLAGLPRPSLAELFADPARLGLLSSKLDLPGGAI
                     RFDWSKTHLDAAHLAGFTALAEAVGFKKARGAMFAGETINVTEGRAVEHTAQRGVGRD
                     SSVEEAAALHARMAGLVAAIHGGALGEVRHLIHIGIGGSALGPALALDALTRDGAVVD
                     VHVVANIDGCALEAAFAKCDPATTLIAVASKTFTTTETMTNAASALAWLRENGVDDPY
                     GRVIALTAYPDKAVEWGVDETRVLPFPETVGGRYSLWSSIGFPIALALGWDEFAEFLD
                     GAAAMDRHFLETDGADNLPLRAAFADLTYARLRGCQTRAVFAYDERLRLFPSYLQQLE
                     MESNGKAVKADGSPVDGPTAPITWGGVGTDAQHAVFQLLHQGTHVVPVDFIACIAAGD
                     ELSPEHHRNLLANCFA"
     gene            1385..2254
                     /gene="lepB"
                     /locus_tag="VFV30_09895"
     CDS             1385..2254
                     /gene="lepB"
                     /locus_tag="VFV30_09895"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX4848446.1"
                     /translation="MNEPVATAEAEPAPEAPEAAPESAAPQPEARKEDSFLVFLLKLV
                     VLVTLFRSFVFSPFNIPSESMLPRLVNGDYLLAAKWPYGYSRYSLPFSLPALPEQRLL
                     ASPPQRGDVVIFKQPPLKELDYIKRVIGLPGDLVQMVDGQLYLNGVPVPKQRIADFEI
                     AISPNTQCYSLQFIAKRADGTDVCRYPRYRETLPGGRYYEVLDLGQRQMDNTEPVMVP
                     EGHLFLMGDNRDNSEDSRFPAEPGRGIGLVPMDNLVGRASVVMWSTDGSVEWFKPWTW
                     FSAARWDRLGGTF"
     gene            2258..>2863
                     /gene="rnc"
                     /locus_tag="VFV30_09900"
     CDS             2258..>2863
                     /gene="rnc"
                     /locus_tag="VFV30_09900"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX4848447.1"
                     /translation="MLAAETHAFLTELADRPPVDEGLWLEALTHGSTGAARDYQRLEF
                     LGDRVLGLVIAEWLHAAETGDEGRLSQRLNALVSRATCARIARSINLGAHIRLGKQAR
                     DDGGADSDNILGDVMEAVIGASFVTHGFDATRAMVRRLWADAVGGQTGQRKHPKSALQ
                     EWCAGNRRRMPDYRLVERTGPDHAARFTVAVSVHGVGEAEAT"
BASE COUNT          499 a          972 c          923 g          469 t
ORIGIN      
        1 gcaaagcagt tggccaggag gttgcggtga tgctcgggcg aaagctcatc accagcggca
       61 atgcaggcga tgaaatcgac tggcaccacg tgcgtcccct ggtgaagcag ctggaacacg
      121 gcgtgctggg catcggtccc caccccgccc caggtgatcg gcgcggtcgg cccatcgacc
      181 gggctgccat cggccttcac cgccttgccg ttcgattcca tttcaagctg ctggaggtag
      241 cttgggaaca gccgcagccg ctcgtcatag gcaaagaccg cgcgggtctg gcagccgcgc
      301 aggcgggcat aggtcaggtc ggcaaaggcg gcgcgcagcg gcaggttgtc cgccccgtcg
      361 gtttccagga aatggcggtc catcgccgcc gcgccatcaa ggaattcggc gaactcatcc
      421 cagcctagcg ccagtgcgat cggaaagccg atcgaagacc acagcgaata gcgcccgccg
      481 acggtctcgg ggaacggcag cacccgggtc tcgtcgacgc cccattcgac cgccttgtca
      541 ggataggccg tcagcgcgat cacccggcca tagggatcgt cgaccccgtt ttcgcgcagc
      601 caagccaagg ccgaggcggc attggtcatg gtttcggtgg tcgtgaaagt cttagaggcg
      661 accgcgatca gcgtggtggc cggatcgcac ttggcaaaag ccgcttccag cgcgcagcca
      721 tcgatattgg cgacaacgtg aacatcgacc accgctccgt cgcgggtcag cgcatcgagc
      781 gccagcgccg gcccaagcgc cgaaccgccg atgccgatgt ggatcagatg tcgcacttcg
      841 cccagcgccc caccatggat cgcggcgacc agcccggcca tccgggcatg gagcgctgcg
      901 gcctcctcaa cactgctgtc gcgcccgacg ccgcgctggg ccgtgtgctc taccgcgcgg
      961 ccttcggtaa cgttgatcgt ctcaccggca aacatcgcgc cgcgcgcctt cttgaagccg
     1021 acagcctcgg ccagcgcagt gaagcctgcg aggtgagcgg catcgaggtg agtcttcgac
     1081 cagtcgaacc ggatcgcccc gccgggcagg tcaagcttgc tcgacagcag gccgagccgc
     1141 gccggatcgg caaacagttc ggccaggctg ggacgcggca ggcctgccag attggtccag
     1201 gctgcggcca cggcttcgct catcatcttg ctccttcagc ttggcgccct aatgcccgtc
     1261 cgcgcccatc tgggaaagag cgataaaatc gtattcgggc cagaccattg ccaccgtcct
     1321 gtccgcgccg cgcgatgaac caagcccctt gacgcgcgcc gctggcgctc cgatggacag
     1381 gataatgaac gaaccggtcg ccactgctga agccgagcct gccccggaag cccctgaagc
     1441 ggcaccggaa agtgcagctc cgcagcccga ggcgcgcaag gaagacagct tcctggtctt
     1501 cctgctgaag ctggtcgtgc tggtcacgct gttccgcagc ttcgtctttt caccgttcaa
     1561 catcccaagt gaatcgatgc tgccgcggct ggtgaacggc gattacctgc tcgccgccaa
     1621 gtggccctat ggctactcgc gctattcgtt gcccttcagc ctgccggccc tgcccgaaca
     1681 gcgcctgctg gccagcccgc cccagcgcgg cgacgtggtg atcttcaagc agccgccgct
     1741 gaaggagttg gactatatca agcgcgtgat cggcctgccc ggcgatctgg tgcagatggt
     1801 cgatggccaa ctctacctca acggcgtgcc ggtgccgaag cagcggatcg ccgatttcga
     1861 gatcgcaatt tcgcccaaca cccagtgcta ttcgctgcaa ttcatcgcca agcgggccga
     1921 cggcaccgat gtctgccgct atccccgcta tcgcgagacc ctgccgggcg ggcgatatta
     1981 cgaggtgctg gatcttggcc agcgccagat ggacaacacc gaaccggtca tggtccccga
     2041 aggccacctg ttcctgatgg gcgacaaccg cgacaattcg gaagacagcc gctttccggc
     2101 cgaaccgggc cgcgggatcg ggctggtgcc gatggacaac ctggttggcc gggccagcgt
     2161 cgtcatgtgg tcaaccgatg gcagtgtcga atggttcaag ccctggacct ggttcagtgc
     2221 tgcgcggtgg gaccgcctgg gcgggacctt ctgagccatg ctggctgccg aaacgcacgc
     2281 tttcctgacc gaactggccg atcgcccgcc ggttgacgaa gggctgtggc tggaagcgct
     2341 gacccacggc tcgaccgggg cggcgcgtga ttaccagcgg ctggagttcc tgggtgaccg
     2401 cgtgctcggt ctcgttatag ccgaatggct gcatgcggcc gagactggtg acgaaggccg
     2461 cctttcacaa cgcctcaacg ccctggtctc gcgcgccacc tgcgcccgga tcgcgcggag
     2521 catcaatctt ggcgcgcaca tccgcctcgg caaacaggcg cgcgacgatg gcggcgcgga
     2581 cagcgacaat atcctgggtg acgtgatgga ggccgtgatc ggcgccagct tcgtcacgca
     2641 cggcttcgac gccacgcggg ccatggtccg ccgcctgtgg gccgatgccg tcggcggcca
     2701 gaccggccag cgcaagcacc ccaagtctgc tctgcaggaa tggtgcgccg gcaatcgccg
     2761 ccgcatgccc gactatcgcc tggtcgaacg caccggcccc gatcacgcgg cgcgctttac
     2821 ggtggccgtc agcgtccacg gcgtgggtga ggcagaggcg acg
//
LOCUS       DASUDV010000458         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1870958,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000458 DASUDV010000000
VERSION     DASUDV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870958"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..816
                     /locus_tag="VFV30_09905"
     CDS             28..816
                     /locus_tag="VFV30_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4848448.1"
                     /translation="MTESDPPLLSLIDGAVAWLTLNRPRAGNSLAMELVEALRAKFAD
                     LAARSDIKVIVLAGAGGRIFSAGHDLNEFNGQDDMDFLARDFAGIAALMQQIMAQPQI
                     VIAKVEGVATAAGCELVAACDLALASSTARFGVPGVNIGFWCHTPQVPLSRTVGRKAA
                     MMMLATGKLFPADHALSIGLINAVHEPEALDAAVDALAAEIASKAGSVLRRGKASFAQ
                     QAALPLAGAYDFARAQALENIVDPDAREGIAAFLEKRQPDWPGG"
     gene            881..2104
                     /locus_tag="VFV30_09910"
     CDS             881..2104
                     /locus_tag="VFV30_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4848449.1"
                     /translation="MHFDVVIVGAGQGGAYAAIQLRQLGFEGTIALIGRESEPPYERP
                     PLSKEYMLGEKEWERLLIRPADFWAGKGIELLLDAEVTTVAASERQVTLGDGRVVGFG
                     ELIWATGGDPRPLSCPGATLAGVHGVRTRADADAILGELAGVSRVCIVGGGYIGLEAA
                     AVLRKLGKEVTLLEVLPRVLARVAGPQLSAFYEQEHRAHGVDLRTETGVVEILGEDRV
                     SGVRLSDDSVVDCQMVIVGIGIIPAVAPLLLAGAAGGNGVDVDEYCRTVLPHVWAIGD
                     CAAHASGFADGAVIRLESVQNANDMASCVAKAITGDPQPYGATPWFWSNQYDLKLQTV
                     GLSIGHDATVLRGDPQARSFSVIYLKAGKMIAIDAVNSVKDYVQARKLVEEGAVIAPE
                     DLADTSRPLKELPRA"
BASE COUNT          347 a          669 c          788 g          358 t
ORIGIN      
        1 tgaacaagac cgcggccaag gtgctcaatg actgagagcg atccgccgct gctttcgctg
       61 atcgatggcg cggtggcctg gctcacgctc aaccgcccgc gcgcgggcaa cagcctggcg
      121 atggaactgg tcgaggcact gcgcgccaag ttcgccgatc tggcggcgcg gtcggacatc
      181 aaggtgatcg tgctggcggg cgcggggggc cggatctttt cggccgggca cgatctcaac
      241 gagttcaacg gccaggacga catggatttt ctggcgcgcg acttcgccgg gattgccgcg
      301 ctgatgcagc agatcatggc ccagccgcag atcgtgatcg ccaaggtcga aggcgtggcg
      361 accgcggcgg ggtgcgaact ggtcgcggcc tgcgatctgg ccctggccag ctcaaccgcg
      421 cgcttcggtg ttccgggcgt caacatcggg ttctggtgcc acaccccgca ggtgccgctc
      481 agccgcaccg tggggcgcaa ggcggcgatg atgatgctgg ccaccggcaa gctgtttccg
      541 gccgatcacg cgctcagcat cgggctgatc aacgcggtgc acgaacccga agcgctcgat
      601 gccgcggtcg atgccctggc ggccgagatc gcctcgaagg cgggatcggt gctgcgccgg
      661 ggcaaggcct cgttcgcgca gcaggccgcg ctgccgctgg ccggggccta tgacttcgcc
      721 cgcgcccagg cgctggagaa catcgtcgat cccgacgccc gtgaaggcat cgccgccttt
      781 cttgagaagc gtcagcctga ctggccgggc ggctaggcgc aatacccttg cgtggcaggc
      841 agcgcgcgct atgggatcgt gccatagcga gggatagcca atgcattttg atgtcgtgat
      901 cgtcggggcc gggcagggcg gggcctatgc cgcgatccag ctgcgccaac tgggtttcga
      961 aggcacgatc gcgctgatcg ggcgcgagag cgaaccgccc tatgagcgtc cgccgctgtc
     1021 caaggaatac atgctgggcg agaaggagtg ggagcggctg ctgatccgcc ccgccgattt
     1081 ctgggccggc aaggggatcg agctgctgct tgacgcagag gtgacgacgg ttgccgcaag
     1141 cgagcgccag gtcacgctgg gcgatggccg ggtggtcggg ttcggcgaac tgatctgggc
     1201 cactggcggc gatccgcgcc cgctttcgtg ccccggcgcg accctcgccg gggtgcacgg
     1261 ggtgcgcacc cgcgccgatg ccgatgcgat cctgggcgag cttgccgggg taagccgggt
     1321 ctgcattgtc ggcggcggct atatcgggct tgaagccgcc gcggtgctgc gcaagctggg
     1381 caaggaagtg accctgctcg aagtgcttcc ccgcgttctg gcgcgcgtgg ccgggcccca
     1441 gctttcggcc ttctatgaac aggaacaccg cgcgcacggg gtcgatctgc gcaccgaaac
     1501 aggcgtggtc gagattctgg gcgaagaccg ggtcagcggg gttcgcctgt cggatgacag
     1561 cgtggtcgat tgccagatgg tgatcgttgg catcggcatc atcccggcgg ttgcgccgtt
     1621 gctgctggcc ggcgctgcgg gcggcaacgg agtggatgtc gatgaatatt gccgcaccgt
     1681 gctgccgcac gtctgggcaa tcggcgactg cgcggcgcat gccagcgggt tcgccgatgg
     1741 agcggtgatc cggctggaaa gcgtgcagaa cgccaatgac atggcgagct gcgttgccaa
     1801 ggcgatcacc ggcgatccgc agccctatgg cgcaaccccg tggttctggt cgaaccagta
     1861 tgatctcaag ctgcagaccg tcgggctgtc gatcggccat gatgcgacgg tgctgcgcgg
     1921 cgatccgcag gcgcgcagct tttcggtaat ttacctcaag gccgggaaga tgatcgcgat
     1981 cgacgcggtc aattcggtca aggactatgt ccaggcgcgc aagctggtcg aggaaggcgc
     2041 ggtgatcgca ccagaagatc tggccgatac cagcaggccg ctgaaggaac tgccgcgggc
     2101 ctaatccccc gcgcggcgat aggccggcag tgccagtcca cgggcgatgg cggctgcgcg
     2161 ca
//
LOCUS       DASUDV010000459         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2158121,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000459 DASUDV010000000
VERSION     DASUDV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158121"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFV30_09915"
     CDS             <1..297
                     /locus_tag="VFV30_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848450.1"
                     /translation="ARTIGKGILPEAVRQRQRGMPADPGHFARLQAFAEPARSRIGAF
                     RAAGIDDWLDLDWLDQTLARVAAQGVSSVVDGNQVQITALAAEYLLWLQEGQAR"
     gene            303..1433
                     /locus_tag="VFV30_09920"
     CDS             303..1433
                     /locus_tag="VFV30_09920"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4848451.1"
                     /translation="MNAILALLWGDPPAAAALADRLGEAEWRTIDRQAGQHRLRPLLH
                     RRAGAGGWNAPAALIEEWQASYQRSALRALGQKAALARIGAALAGGGLSAAVLKGGVL
                     VWSDGFDPALRPMRDLDLLVRPETAEAAAAALRQIGFTGGALPQAKGAKHLEPMVSGR
                     VAVELHLHLFDSADEQAAARESQFIARAWQRAEPGTVPGLLELAPTDTLLHLILHAVI
                     DHQFNNGPLLLGDMQALVGAGRIDWPLFWQEAAALEATRACQLALRIGQQVCALPVDW
                     HGHEPADLSPAELDAALRLMLVDTGHRSAVGWPGQLLRLAPHRWPAQLAAMLARRRNR
                     AAGEPGAGGGAGLGAAIGHAVGAEGRKSIADAVRLALWLRRG"
     gene            complement(1434..1874)
                     /locus_tag="VFV30_09925"
     CDS             complement(1434..1874)
                     /locus_tag="VFV30_09925"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX4848452.1"
                     /translation="MAARLARLRRKWRAWRGYAFADRLLIVEAAVWLAAARLFVLLLP
                     FRWLGPWLRKTTTPPDRPALAPRIGRAVLTASRHVPWQAACLPQALAAKLMLGLRGQG
                     AVVHLGAKLDETGALAAHAWLTCGDNLVTGGEGLPGYTPLIQLG"
     gene            complement(1879..2943)
                     /locus_tag="VFV30_09930"
     CDS             complement(1879..2943)
                     /locus_tag="VFV30_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4848453.1"
                     /translation="MDNDRVLLAKAHAPLADAARGNPRALLALAANAQRDGALQRCHE
                     LVQEALALGSDDPEIAAAARTIMGQAVPAWHFPMMRDDIRNQAFQDAIERAVAPGMKV
                     LDIGSGSGLLAMMAARAGAGEVHSCELNPVIAATAREIVARNGYADTITVHARNSAKL
                     DAEADMGGPADLVISEIIGKDLVCEAVLPSMRDAVRRLAKPGAQVIPRRGEIRVALAH
                     YAKLEALSVDNVCGFDLTPFNRLRRARIDVQARDPALTLRGEPAVLFDFDFASADPHP
                     DRSALNLVASGGTANGVVQWFRLQMDEAGRFENPPGADSSRSWALVFFPFDQPLELEP
                     GEQVAVAAKVVNNLLRVWQA"
     gene            3108..>4019
                     /locus_tag="VFV30_09935"
     CDS             3108..>4019
                     /locus_tag="VFV30_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX4848454.1"
                     /translation="MTPLSHHDVADDIRPLLAGLASGAERFAIATLVSTDGPSPRDAG
                     AQMLVTDADRWGFLSGGCVEDDVARHAREVIASGSPRLLRYGAGSPWIDIKLACGAGI
                     SILVEPATLDDPAVATLLAGYRERQPVLWQSDGAARRASFCEEASPNLPLWDGHSFSD
                     LFEPPLRLVIVGSDATALALAGLALHLGVETVLVNPGGPATAPFPGMGYLRTGAAEVL
                     PLDRWTAVAVVTHDRDGDETLLAAALASDALYVGAIGARSRLQGRQERLQQLGLSAEA
                     IARLRAPIGLHGLGKSPRAIALSILSEV"
BASE COUNT          585 a         1432 c         1405 g          597 t
ORIGIN      
        1 gcccgcacga tcggcaaggg cattcttccc gaagcggtgc gccagcgcca gcgcggcatg
       61 ccagccgatc cgggccactt cgcccggctg caagcctttg ccgaaccggc gcggagccgg
      121 atcggggcct tccgcgcggc cgggatcgat gactggctgg acctcgactg gctggaccag
      181 accctggcgc gggtcgcggc ccagggcgta tcctcggtcg tcgatggcaa ccaggtgcaa
      241 atcacggccc tggccgcaga ataccttctc tggctgcagg aaggccaagc ccggtgagcg
      301 ggatgaacgc gatccttgcc ctgctgtggg gggatccgcc tgccgcagcc gcgcttgccg
      361 atcggctggg cgaggccgag tggcggacga tcgaccgcca ggccggccag caccgcctgc
      421 ggccgctgct tcaccgccgc gccggggctg gcggatggaa cgcccccgcg gcgctgatcg
      481 aggaatggca ggcaagctat cagcgctcgg ccctgcgggc gctggggcaa aaggccgccc
      541 tcgcccggat cggcgccgcg cttgccgggg gcggactgtc ggccgccgtg ctcaagggcg
      601 gggtcctggt ctggtccgac gggtttgacc cggcgcttcg cccgatgcgc gatctggacc
      661 tgctggttcg ccccgaaacc gcagaggccg ccgctgcggc gctgcgccag atcggcttta
      721 ccggcggcgc cctgccccag gccaaagggg cgaagcacct tgagccgatg gtcagcgggc
      781 gggtcgcggt tgagctgcac ctgcacctgt ttgacagcgc ggacgagcag gccgcggcgc
      841 gcgaaagcca attcatcgcc cgcgcctggc agcgtgccga gccgggcacc gtgcccggac
      901 tgctcgaatt ggcgccgact gacaccctgc ttcacctgat cctccacgcg gtgatcgacc
      961 atcagttcaa caacggcccg ctgctgctgg gcgacatgca ggcgctggtc ggcgcgggcc
     1021 ggatcgattg gccgctgttc tggcaagagg ccgcggcgct ggaggcaacc cgcgcttgcc
     1081 agctcgcgct gaggatcggc cagcaggtct gcgcgctgcc ggtggactgg cacgggcacg
     1141 agcccgccga tctcagccca gcggagctgg acgcggcctt gcggttgatg ctggtcgata
     1201 ccgggcaccg ctcggccgtt ggctggccgg ggcagctgct gcggctggcc ccgcaccgct
     1261 ggccggccca gctggccgcc atgctggccc ggcggcgcaa ccgcgcggcg ggggaaccgg
     1321 gagcgggagg cggtgccggg cttggcgcgg cgatcggcca tgccgtgggt gccgaggggc
     1381 gcaagagcat tgccgatgcc gtgcgcctgg ccctgtggct gcggcgcggt tgatcagccg
     1441 agctggatca agggggtgta gcccggaagc ccctctccgc cggtcaccag attgtcgccg
     1501 caggtcagcc aggcatgggc ggcaagcgcg cccgtttcgt ccagcttggc gcccaggtgc
     1561 acgaccgcgc cctgcccgcg cagccccagc atcagctttg cggccagggc ctgcggcagg
     1621 cacgccgcct gccacggcac gtggcgcgat gcggtgagca ccgcgcgccc gatccgcggg
     1681 gcaagcgccg gccggtccgg cggggtggtg gtcttgcgca gccacgggcc cagccagcgg
     1741 aacggcaaga gcagcacgaa cagccgcgct gctgccagcc agacggccgc ttccacgatc
     1801 agcaggcgat cggcaaaggc ataaccgcgc caggcgcgcc attttctgcg aagccgggcc
     1861 agccgtgccg ccatggcttc aggcctgcca gacccgcagc aggttgttca cgaccttggc
     1921 cgccaccgcc acctgctcgc ccggttccag ctccagcggc tggtcaaagg ggaagaacac
     1981 cagcgcccag gaccgcgacg aatccgcccc cggcggattt tcgaagcggc ccgcctcgtc
     2041 catctgcagc cggaaccatt gcaccacacc gttggcggtt ccgccgctgg cgaccaggtt
     2101 cagcgcgctg cgatcggggt gcgggtccgc actcgcgaaa tcgaaatcga acaggacggc
     2161 cggctcgccg cgcagcgtca gcgcgggatc gcgcgcctgc acgtcgatcc gcgcgcgccg
     2221 cagccggttg aacggcgtga gatcgaagcc gcagacgttg tccaccgaaa gcgcttcaag
     2281 ctttgcatag tgggccaggg caacgcggat ctcgccccgc cgcgggatca cttgcgcgcc
     2341 gggcttggcc agccgccgca ccgcgtcgcg catcgaaggc agcaccgcct cgcagaccag
     2401 gtccttgccg atgatttccg aaatcaccag gtcggccggc ccgcccatat ccgcttcggc
     2461 atccagcttg gccgaattcc gggcgtggac agtgatggta tcggcatagc cgttgcgcgc
     2521 gacaatctct ctcgcggtgg cggcgatgac cgggttgagt tcgcacgagt gcacctcccc
     2581 cgccccggcg cgcgccgcca tcatggccag caggcccgat cccgagccga tatccagcac
     2641 cttcatcccc ggggcaaccg cccgctcgat ggcgtcctgg aaggcctggt tgcggatatc
     2701 gtcgcgcatc atcggaaagt gccaggcagg cacggcctgg cccataatgg tccgcgcggc
     2761 tgccgcaatc tcgggatcgt cgctccccag cgccaacgcc tcctgcacca gctcgtggca
     2821 ccgctgcaac gcgccgtcgc gctgcgcgtt cgccgccaag gccagcaagg cacgggggtt
     2881 gcccctggcc gcatcggcca gcggcgcgtg cgcctttgca agcagaacgc ggtcgttatc
     2941 catgcccgtc acttaggcga ggcccatgaa gtgtaaagcg ccaattgcca cggccccagc
     3001 ggaacccgcg gccccttttg gatcgcaaga cctgaaccga tgactggaca gcaatcccgt
     3061 ttcccgcaaa catcgtgctt ctgcatccgc cacgggacca tccagctttg acaccgctca
     3121 gccatcacga cgttgcggac gatatccgtc cgctcttggc ggggctggcc tccggtgcgg
     3181 agcgctttgc cattgcgacg ctggtttcca ccgatggccc ctcgccgcgc gatgcgggcg
     3241 cgcagatgct ggtgacggac gccgatcgct gggggtttct gtccggtggc tgcgttgaag
     3301 acgatgtcgc ccgccacgcg cgcgaggtga tcgcctcggg tagcccgcgc ctgctccgct
     3361 atggtgcagg cagcccgtgg attgacatca agctggcctg cggtgcgggc atttcgatcc
     3421 tggtcgagcc tgcgacgctt gacgatccgg cggttgccac gctgctggcc ggataccgcg
     3481 aacgccagcc agtcctctgg caaagcgacg gcgcggcgcg cagggccagc ttctgcgaag
     3541 aggccagccc gaacttgccg ctgtgggacg gccacagctt ctccgacctg tttgaaccgc
     3601 ccttgcggct ggtcatcgtc ggcagcgatg caaccgcgct ggcgctcgcc gggctggcgc
     3661 tgcatctggg ggtggaaaca gtgctggtga accccggcgg gccggcgacc gcgccctttc
     3721 ccggcatggg ttacctgcgc accggcgcgg ccgaggtcct gccgctcgat cgctggacag
     3781 cggtggccgt ggtcacccac gatcgcgacg gcgacgagac cctgctggcc gcggccctgg
     3841 cgtcagacgc cctttatgtc ggcgcgatcg gggcccgcag ccggctgcaa ggccggcagg
     3901 agcgcttgca gcaactgggc ttgagtgccg aggcgatcgc gcggctgcgc gcgccgatcg
     3961 ggcttcacgg gctgggcaaa tcgccgcggg ccatcgcgct gtcgatcctg tccgaagta
//
LOCUS       DASUDV010000460         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_987322,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000460 DASUDV010000000
VERSION     DASUDV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987322"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFV30_09940"
     CDS             <1..889
                     /locus_tag="VFV30_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848455.1"
                     /translation="AAPAAPVAAPLPSPAGLGMTDLASRLAESMARRRAARAGAAAPE
                     APAASAPAAAFEPAPPPPAFAPAPVEAPAPIPAAFTPAETPAPETFAAPFAAPQPAMP
                     AAFAPPSVEAAVPPAVQPPIPVAFEAPQPLPAVPSAMRPLDLGGFEEEEAPLDSLLPP
                     RLNAAAPAPQPAPVMPAVPDEAELAAQGVQEDNYASLLGIGAFAPRGGPVRIDEPELE
                     QAATEPVVIFPGQAPRPVEAPAMADPNGGFRRFDAPASAGQGQPIAANQAAPSVAPDE
                     AEQALRAALANLQRMSGAA"
     gene            1126..>3848
                     /locus_tag="VFV30_09945"
     CDS             1126..>3848
                     /locus_tag="VFV30_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX4848456.1"
                     /translation="MGFPTPQGLYDPRNEHDACGVGFVAHIKGTKSHAIIAQALEILK
                     NLDHRGAVGADPLLGDGAGILIQLPDALYRQWAEAEGLKLPPEGDYAVAMCFLPQDEA
                     SREFIVGMFEKFIAKEGQRLIGWRDVPVTTEGLGKTVLEQMPVIRQCFVARGENCADQ
                     DAFERKILAIRKQTQNPLAALAEKHGLPGLTELYMPSFSSRTVVYKGLLLATQVGSFY
                     DDLRHPACVSALGLVHQRFSTNTFPSWKLAHPYRFIAHNGEINTVRGNVNWMEARRRT
                     MESELLGPDLDKMWPIIPHGQSDTACLDNALELLMAGGYSLAHAVMMLIPEAWAGNPL
                     MDAKRRAFYEYHAALMEPWDGPAAVAFTDGRQIGATLDRNGLRPARFLVTDDDLVVMA
                     SESGVLPIKEDSIVRKWRLQPGRMLLIDFAEGRIIEDEELKTQLSAEQPYEDWLEAAQ
                     YKLKDLEVVEPELAQLAVETTSLLDRQQAFGYTQEDVAKFLEPMGRDGDDPIGSMGTD
                     TPIAVLSNRSRLLYDYFKQNFAQVTNPPIDPIREELVMSLVSMIGPRPNLLGMEAGTH
                     KRLEVDQPILTNDEVAKIRSVEAALDGAFRTETIDITWDAASGADGLEMALKEMCWAA
                     TEAVLQDKNILILSDRAQGPDRIPMPALLATAAVHHHLVRQGLRMQTGLVVETGEARE
                     VHHFCVLAGYGAEAINPYVAFETLEEIRVKKDLPLSAKEVQKNYIKAVGKGILKVMSK
                     MGISTYQSYCGAQIFDAVGLSSAFIDKYFTGTATTIEGIGLAQVAEEAVRRHRAAYGD
                     NPIYKSMLDVGGMYGLRLRGEEHAWTADNIAALQHAVRGNVPDKYKAFAQSINDQSSR
                     MRTIRGLMEFKPAERPLDVSEVEPASEIVKRFATGAMSYGSISWEAHTTLAL"
BASE COUNT          642 a         1375 c         1263 g          568 t
ORIGIN      
        1 ggctgcgccc gccgcgccgg tggccgcgcc gctgcccagc ccggccgggc ttggcatgac
       61 cgacctggcc tcgcgcctgg ccgaatcgat ggcgcgccgc cgcgccgccc gcgccggtgc
      121 cgccgcgccg gaggctccgg ccgcgtccgc tccggcggcc gcgttcgaac ccgctccccc
      181 gccgcccgcc ttcgcgccgg ctccggtcga ggccccggct ccgattccgg ccgcctttac
      241 gccggccgag accccggccc cggaaacctt tgccgcgccg ttcgccgcgc ctcagccggc
      301 gatgcccgcc gccttcgcgc cgccttcggt tgaagccgcc gtcccgcctg ccgtccagcc
      361 gcccattccg gtggcctttg aggctccgca gccgctcccg gcggttcctt cggccatgcg
      421 cccgctcgat cttggcgggt tcgaggaaga ggaagccccg ctcgacagcc ttctgccgcc
      481 gcggctcaac gccgctgcgc cggccccgca gcccgcaccg gtcatgcccg ccgtgcccga
      541 cgaagccgaa ctggccgcgc agggcgtgca ggaagacaat tacgcctcgc tgcttggcat
      601 cggtgccttt gcgccccgcg gcggtccggt ccggatcgac gagcccgagc tggagcaggc
      661 cgcgaccgaa ccggtggtga tcttccccgg ccaggccccg cgcccggtcg aagccccggc
      721 gatggccgat ccgaacggcg ggttccggcg gttcgacgcc ccggccagtg ccggtcaggg
      781 ccagccgatc gccgccaacc aggcggcccc ttcggtcgcc ccggacgagg ccgaacaggc
      841 cctgcgcgcg gcgctggcca acctccagcg gatgagcggc gcggcctgat tccggccggc
      901 cgcccggcca ccgaaagcct ctgcatagct atgggccggc cgccacgcgc ggaccggccc
      961 ttttcgcagt tgcaaaaaca gcttcctgtc gccatgggat agttctgcgc aattttcgct
     1021 gcttttcgcg gcaaggaatc ttgcgcttaa ttgccggaat tctgcgcttt gcggggcaca
     1081 gcccttcgaa tcgcggcatt ccgcccggtg acaggatggg attcgatggg ttttccgacg
     1141 cctcagggcc tctatgatcc gcgtaacgaa cacgacgctt gcggcgtggg cttcgtcgcc
     1201 cacatcaagg gcacgaaaag ccatgcgatc attgcccagg cgctcgaaat cctgaaaaac
     1261 ctcgatcacc gcggcgcggt tggcgccgac ccgctgctgg gtgacggcgc gggcatcctg
     1321 atccagctgc ccgacgcgct gtaccgccaa tgggccgagg ccgaggggct gaagctgccc
     1381 cccgaaggcg actatgccgt ggccatgtgc ttcctcccgc aggacgaggc gagccgcgag
     1441 ttcatcgtcg ggatgttcga aaagttcatc gccaaggaag gccagcgcct gatcggctgg
     1501 cgcgacgtgc cggtgaccac cgagggcctg ggcaagaccg tgctggagca gatgccggtg
     1561 atccgccagt gcttcgtcgc gcggggcgag aactgcgccg atcaggacgc gttcgagcgc
     1621 aagatcctgg cgatccgcaa gcagacccag aacccgctgg cggcgctggc cgaaaagcac
     1681 ggcctgcccg gcctgaccga gctctacatg ccgagcttct ccagccggac cgtggtctac
     1741 aaggggctgc tgctggcaac ccaggtcggc tcgttctatg acgatctgcg ccacccggcc
     1801 tgcgtttcgg cgctgggcct ggtccaccag cggttcagca ccaacacctt ccccagctgg
     1861 aagctggccc acccctatcg cttcatcgcc cacaacggcg aaatcaacac cgtgcgcggc
     1921 aacgtcaact ggatggaagc ccgccgccgg acgatggaaa gcgaactgct cggcccggac
     1981 ctcgacaaga tgtggccgat catcccgcac ggccagtcgg acacggcctg cctcgacaac
     2041 gcgctcgagc tgctgatggc cggcggctac agcctggccc acgcggtgat gatgctgatt
     2101 ccggaggcct gggccggcaa cccgctgatg gatgccaagc gccgcgcctt ctacgaatac
     2161 catgccgcgt tgatggagcc gtgggacggc ccggccgccg tggcctttac cgacggccgc
     2221 cagatcggcg ccacgctgga ccgcaacggc ctgcggcccg cgcggttcct ggtgaccgac
     2281 gacgacctgg tggtgatggc ctccgaaagc ggggtcctgc cgatcaagga agacagcatc
     2341 gtgcgcaagt ggcgcctgca gcccgggcgc atgctgctga tcgactttgc cgaagggcgg
     2401 atcatcgagg acgaggagct gaagacccag ctttcggccg agcagcccta tgaggactgg
     2461 ctggaagccg cgcagtacaa gctcaaggac ctggaagtgg tcgagcccga gctggcccag
     2521 ctggcggtgg aaaccaccag cctgctcgat cgccagcagg cctttggcta tacccaggaa
     2581 gacgtcgcca agttccttga gccgatgggc cgggatggcg acgatccgat cgggtcgatg
     2641 ggaaccgaca ccccgatcgc ggtgctctcc aaccgcagcc gcctgctcta cgattacttc
     2701 aagcagaact tcgcccaggt caccaacccg ccgatcgatc cgatccgcga ggagctggtg
     2761 atgagcctgg tctcgatgat cggcccgcgc cccaacctgc tgggtatgga agccggcacc
     2821 cacaagcggc ttgaggtcga ccagccgatc ctgaccaacg acgaggtcgc caagatccgc
     2881 tcggtcgagg ccgcgctgga tggcgccttc cggaccgaga cgatcgacat cacctgggat
     2941 gccgccagcg gggcggacgg cctggaaatg gcgctcaagg aaatgtgctg ggccgcgacc
     3001 gaggccgtgc tgcaagacaa gaacatcctg atcctgtccg accgtgccca gggcccggac
     3061 cggattccga tgccggcgct gctggccacc gccgcggtcc accaccacct ggtccgccag
     3121 ggcctgcgga tgcagaccgg gctggtcgtc gagaccggcg aagcgcgcga agtgcaccac
     3181 ttctgcgtcc tggccggcta cggcgccgaa gcgatcaacc cctatgtggc cttcgaaacg
     3241 ctggaggaaa tccgcgtcaa gaaggacctg cccctgtccg ccaaggaggt gcagaagaac
     3301 tacatcaagg cggtcggcaa gggcatcctc aaggtcatgt ccaagatggg catctcgacc
     3361 taccagtcgt actgcggcgc gcagatcttc gacgcggtcg gcctgtcgag cgcgttcatc
     3421 gacaagtact tcaccggcac ggcgaccacg atcgagggga tcggcctggc ccaggtggcc
     3481 gaggaagcgg tgcggcgcca ccgcgcggcc tatggcgaca acccgatcta caagtcgatg
     3541 ctggacgtcg gcgggatgta cggtctgcgc ctgcgcggcg aggagcacgc ctggaccgcc
     3601 gacaacatcg ccgccctgca gcacgcggtg cgcggcaacg tgccggacaa gtacaaggcc
     3661 ttcgcccagt cgatcaacga ccagtcgagc cggatgcgga cgatccgcgg gctgatggaa
     3721 ttcaagccgg ccgagcgccc gctggacgtc agcgaggtcg aaccggccag cgaaatcgtc
     3781 aagcgctttg ccaccggggc gatgtcctat ggctcgatca gctgggaggc ccacaccacc
     3841 ctggcgct
//
LOCUS       DASUDV010000461         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_170396,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000461 DASUDV010000000
VERSION     DASUDV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170396"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /gene="mraY"
                     /locus_tag="VFV30_09950"
     CDS             <1..174
                     /gene="mraY"
                     /locus_tag="VFV30_09950"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX4848457.1"
                     /translation="VIIQVAVYKRTGKRVFKMAPIHHHFEQLGWKESTVVIRFWIVSI
                     ILALIGLSTLKLR"
     gene            171..1520
                     /gene="murD"
                     /locus_tag="VFV30_09955"
     CDS             171..1520
                     /gene="murD"
                     /locus_tag="VFV30_09955"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX4848458.1"
                     /translation="MITTSAFAGKRYAVLGLARSGMAAVESLLAGGAQVTAWDSREEP
                     RRALEGRCELADPVGIDLTGFAGVVVSPGVPLNTHPIAKAAEAAGVPVIGDIELFALA
                     RPELPAHRVVGITGTNGKSTCTALVHHILQSAALPTRMGGNIGLPILGANPLDAGGVY
                     VLELSSYQIDLTHSLQCDVAALLNITPDHLDRYDGFAGYAASKARLFAMQRADQFAVF
                     GMGDAKTQAVAARELQTRAPGKVRSVDGRDLAALQPDWPSLQGPHNLQNAAVAVAIAE
                     ALGLTAMQWRPALASFRGLPHRMERVAEANGVMYINDSKATNPASTAPALAAFPPNPD
                     RRVHWILGGLPKGDDLDECAPFFGNIAAAYTIGDAGPRFAELLSPHMPVTRSEMMCEA
                     IQQAMAAAKPGDVVLLSPACASFDQFRDYEARGDAFRQIVQTLLTPEPAGAAGGARS"
     gene            1520..>2349
                     /locus_tag="VFV30_09960"
     CDS             1520..>2349
                     /locus_tag="VFV30_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX4848459.1"
                     /translation="MASNPPLVPRGGKVGVEGGAVRAKRNRRSELTIWWREIDRTLLA
                     LVLTLMAMGAAAVAASSPASARRLSTASVRLDDLHFFYLHIRYQILGLIAMFGASMLP
                     RDMARRAGIVLCCAMLVGLMLVPLIGFSVNGARRWLNLGMSLQPSEFLKPAFAIGMAW
                     ILSWRARDPNLPVIAITTALMGLIAGLLMMQPDFGSAILFIGVWFVMVLMSGISLQRI
                     GLAGGAGLAFVVLAYMFYENARNRIDSFLGGGTAFDQVDLAQRTLLAGGWTGSGLWLG
                     T"
BASE COUNT          331 a          814 c          805 g          399 t
ORIGIN      
        1 gtgatcatcc aggtcgcggt ctacaagcgc accggcaagc gggtcttcaa gatggccccg
       61 atccaccacc atttcgaaca gctgggctgg aaggaatcga ccgtcgtgat ccgcttctgg
      121 atcgtctcga tcatcctcgc cctgatcgga ctgtccaccc tgaagctgcg atgatcacca
      181 cctccgcctt tgccggcaag cgctatgccg tcctcgggct cgcccgctcg gggatggcgg
      241 ctgtcgaaag cctgctggcg ggcggcgcgc aggttaccgc ctgggacagc cgcgaagagc
      301 cgcgccgggc actggaaggc cgctgcgagc tggccgatcc ggtggggatc gacctgaccg
      361 gctttgccgg agtggtcgtc tcgccggggg tgccgctcaa cacccatccg attgccaagg
      421 cggccgaagc cgccggggtt ccggtgatcg gcgatatcga actgttcgcg cttgcgcggc
      481 ccgagctgcc cgcgcaccgg gtggtcggga tcaccggcac caacggcaag tcgacctgca
      541 ccgcgctggt ccaccacatc ctgcaatcgg ccgccctgcc gacccggatg ggcggcaaca
      601 tcggcttgcc gatcctgggc gccaatccgc tcgacgcggg cggggtctat gtgctggagc
      661 tgtcgagcta ccagatcgac ctgacccatt cgctgcagtg cgatgtcgcc gcgctgctca
      721 acatcacgcc cgatcatctg gaccggtacg atggctttgc cggctatgcc gcgtcgaagg
      781 cgcggctgtt cgcgatgcag cgggccgatc agttcgcggt gttcggcatg ggcgatgcga
      841 agacccaggc ggtcgccgcg cgcgagcttc agacccgcgc gccgggcaag gtccgcagtg
      901 tcgacgggcg ggacctcgcc gcgctgcagc ctgattggcc cagcctgcag ggtccgcaca
      961 acctgcagaa cgccgccgtg gccgtggcga ttgccgaagc cctggggctg accgcgatgc
     1021 aatggcgccc ggcgctggcc agcttccgcg gcctgccgca ccggatggag cgcgtcgccg
     1081 aagcgaacgg ggtgatgtac atcaatgaca gcaaggcgac caacccggcc tcgaccgcgc
     1141 cggcgctggc cgccttcccg cccaatccgg accggcgggt ccactggatc ctgggcggcc
     1201 tgcccaaggg cgacgacctt gacgaatgcg cgccgttctt tggcaacatc gccgcggcct
     1261 ataccatcgg cgatgccggc ccgcgctttg ccgagctgct cagcccgcac atgccggtga
     1321 cccgcagcga gatgatgtgc gaggcgatcc agcaggccat ggccgcggcc aagcccggcg
     1381 atgtcgtgct gctctccccg gcttgcgcca gctttgacca gttccgcgat tacgaggcgc
     1441 gcggcgatgc ctttcgccag atcgtccaga ccctcctgac ccccgaaccc gccggggccg
     1501 cgggcggagc gcgcagctga tggcgagcaa tccccccctg gtgccgcgcg gcggcaaggt
     1561 cggcgtcgaa ggcggcgcgg tgcgcgccaa gcgcaaccgc cgcagcgagc tgacgatctg
     1621 gtggcgcgag atcgaccgga ccctgctggc gctggtcctt acgctgatgg cgatgggcgc
     1681 ggcggcggtg gcggccagtt cgccggccag tgcgcggcgc ctgtcgaccg cttcggtccg
     1741 gctcgatgac ttgcacttct tctacctcca catccgctac cagatcctgg gcctgatcgc
     1801 gatgttcggg gccagcatgc tgccgcgcga catggcgcgc cgcgccggga tcgtgctgtg
     1861 ctgcgccatg ctggtggggc tgatgctggt tccgctgatt ggcttcagcg tcaacggcgc
     1921 gcggcgctgg ctgaacctgg ggatgtcgct ccagccctcg gaattcctca agccggcctt
     1981 tgccatcggc atggcctgga ttctctcgtg gcgcgcgcgc gatccgaacc tgccggtgat
     2041 tgccatcacc acggcgctga tggggctgat cgccggcctg ctgatgatgc agcccgattt
     2101 cggctcggcc atcctgttca tcggggtctg gttcgtgatg gtgctgatga gcggcatctc
     2161 gctccagcgg atcggcctcg ctggcggggc ggggctggcc ttcgtcgtgc tggcctacat
     2221 gttctacgaa aacgcccgca accggatcga cagcttcctg ggcggcggca ccgcgttcga
     2281 tcaggttgat ctggcccagc ggaccctgct ggccggcggc tggaccggat cgggtctgtg
     2341 gctcggcac
//
LOCUS       DASUDV010000462         5983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1407555,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000462 DASUDV010000000
VERSION     DASUDV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407555"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..826)
                     /locus_tag="VFV30_09965"
     CDS             complement(149..826)
                     /locus_tag="VFV30_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX4848460.1"
                     /translation="MVRSKRKPFTPATVAVIGLGRFGTSVARSLAQLGHEVLAIDSDP
                     RNVQLLADELPHILQADTTDPDALHQIGISDCERVVVGIGSDVEASVLTVMGLTDLGI
                     PQIWAKATHRTHGRILERVGAHHVVYPEESMGRRVARLVTGKMIDQIEFDEGFSIVKT
                     RAPQELVGSVLGIIDTMQKYGVAIIGVKPEGEPFRHVTQDIIVQAGDLLIIAGETDEC
                     EAFAALD"
     gene            complement(819..2207)
                     /locus_tag="VFV30_09970"
     CDS             complement(819..2207)
                     /locus_tag="VFV30_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX4848461.1"
                     /translation="MSRSQPPSPGLAARSTMQRQFHPTRIVPLAFLAAIALGTLVLML
                     PFSTVDGQGAGFMTALFTATSAICVTGLIVVDTPTYWSGAGQAAILLMIKIGGLGIMT
                     AATLLGLVAGRRLSLSSRAVALTEHGHLNRAEAASLLKLIFVVSVAIEAVIAFSLWLR
                     WWIGHGMAPAEAAWHAVFHAVSAFNNAGFSTFSDNLMSWHRDGAILLPIMLAIVIGGI
                     GFPVLHEIWTRRDPARRQRPWSLHSRLTITTTLVLLIGGTVLMLAAEWSNPATFGAMT
                     PGERVLGAAFHSVSMRTAGFNAIDIGGIANPSYLVNFLFMMIGGGSAGTAGGLKVTTF
                     AVLGYVVWSEIRGHADTNLFGRRLGAAVVRQAVSVALLAIALIGMAGFVLTMLEPAIA
                     PHKLLFEAISAFATVGLSANITALLSTPSQLVLVLLMFVGRVGTITFATALALAARSN
                     HYRYPEEQPIVG"
     gene            2359..2760
                     /locus_tag="VFV30_09975"
     CDS             2359..2760
                     /locus_tag="VFV30_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX4848462.1"
                     /translation="MGFFGKIFTWWNGATVTTLLNSALTGEKVGTDAQGNTYYRAKKP
                     YPKGHPFAGKERRWVIYNGANDASRVPAEWHGWLHGSFEGVPESNLPPPRIWEVDYTP
                     NPTGTPNAHRPQYSKGGGRKDGAYEAWSPDA"
     gene            2760..3161
                     /locus_tag="VFV30_09980"
                     /pseudo
     CDS             2760..3161
                     /locus_tag="VFV30_09980"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(3197..3979)
                     /gene="aat"
                     /locus_tag="VFV30_09985"
     CDS             complement(3197..3979)
                     /gene="aat"
                     /locus_tag="VFV30_09985"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX4848463.1"
                     /translation="MLMLAYRTGVFPMADARDDPEIFWVEPRRRAILPLDGFHCSHSL
                     AKALKRGTFRVTCNAAFDAVITECAGPRRDPGGTWISDRIERTYRELHRLGHAHSIEC
                     WQGEELVGGLYGVGFDRVFCGESMFSRRSDASKVALAWLVAALRRAGAVLLDCQFMTD
                     HLASLGAVEISQSDYASRLRAAQSPTLGDGALAGGALAGGALGAAEGLGEGDAVGEAA
                     GEAAGAALALPAGFSALLADAAAAGLSSSPGNFIAQSFTQTS"
     gene            4093..4168
                     /locus_tag="VFV30_09990"
     tRNA            4093..4168
                     /locus_tag="VFV30_09990"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4126..4128,aa:Trp,seq:cca)
     gene            4196..5701
                     /locus_tag="VFV30_09995"
     CDS             4196..5701
                     /locus_tag="VFV30_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848464.1"
                     /translation="MPEAAPPVPSPSPRAGLRQWLTAEPGPREFAGLAFVLALLSALP
                     VLVATYPQMVDYPAHLARMHIMLTRDQSPFLQEYYGFEWRWMGNLGADLLVIPLTWFV
                     SLETAGRIMAGIIPPLTALSLMAAEWAVRRRIGMASLLAFAFIWSPSAILGFLNFGLA
                     QAGALFAFALWVLLDGKSWRAPLFIPIGIFVWLGHVSGWGILGILVFGYEWSKDKSWR
                     AFLAPWPLFFPFLSLILGDNPGKPPSYGPAPQLYKWAIWKQAMRGTLQWVDYAMAIGV
                     CLLLAASLAWRKWDGKLGWAAAMMLVSSLVLPRHIFGGDLVDARMISAGLMVGCLCLA
                     WRAPRWIVLLAPALFLSRLAYTTLDWERDSRETAQALTALEGLPKGAKVASYVVTERS
                     EWGFNGDEHICGYAVVRLDAFTNCNFALPGIHMMTINGGGPFFRDPFHRLNHRAGLKI
                     DISDYNPAKYTDYLWYVGQSDPVKLPPGFKVEQRGRTWFLARQTGKRPDTE"
     gene            5877..>5983
                     /gene="secE"
                     /locus_tag="VFV30_10000"
     CDS             5877..>5983
                     /gene="secE"
                     /locus_tag="VFV30_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX4848465.1"
                     /translation="MAKFNPGEFMRQVQTEARKVVWPSRKETTTTAIFVA"
BASE COUNT          998 a         2050 c         1993 g          942 t
ORIGIN      
        1 gagacccgcc cggtccgcgc gcggatgtgg ctggtcaagc gggaccgcta tttcgtgacg
       61 gtcacggcct ccggcccggc cgacgacgat ttcaaggaac tggacgcgat cgccaattcg
      121 ctggtgctga ccaagtaacc gccgctgcct agtccagcgc ggcgaaggcc tcgcactcgt
      181 cggtttcgcc ggcgatgatc agcaggtcgc ccgcctggac gatgatgtcc tgggtgacgt
      241 gccggaacgg ctcgccctcg ggctttacgc cgatgatggc gacgccgtac ttttgcatcg
      301 tgtcgatgat gcccagcacg ctgccgacca gctcttgcgg agcgcgggtc ttcacgatcg
      361 agaagccctc gtcgaattcg atctggtcga tcatcttgcc ggtcaccagc cgggccacgc
      421 ggcggcccat gctttcttcc ggatagacca cgtggtgcgc gccaacccgc tccaggatgc
      481 ggccgtgggt gcggtgggtg gccttggccc agatctgcgg gatgccaagg tcggtcagcc
      541 ccatcacggt cagcacgctc gcctcgacat cgctgccgat gccgaccacc acccgctcgc
      601 aatcgctgat cccgatctgg tgcagggcat cggggtcggt cgtatcggcc tgcaggatat
      661 gcggcagctc gtcggccagc agctggacgt tgcggggatc ggaatcgatc gccagcacct
      721 cgtgccccag ctgcgccagc gagcgcgcaa ccgacgttcc gaaccgtccc aggccgatca
      781 ccgcgacagt ggcgggggtg aagggtttgc gctttgacct aaccaacaat gggctgctcc
      841 tcgggataac ggtagtggtt ggaccgcgcg gccagcgcca gggcggtcgc gaaggtaatc
      901 gtgccgaccc ggccgacgaa catcagaagg accagcacca gctgcgacgg agtggacagc
      961 agcgccgtga tgttggccga caggccgacc gtggcaaagg cggaaatggc ctcgaacagc
     1021 agcttgtgcg gcgcgatcgc gggctccagc atggtcagga caaagccggc catgccgatc
     1081 agggcaatcg ccagcaaggc caccgacacg gcctgccgca ccaccgccgc gcccagccgc
     1141 cgcccgaaca ggttggtatc ggcatggccg cggatttccg accacacgac atagcccagc
     1201 acggcaaagg tcgtcacttt caggccgccg gccgtccccg cgctgccgcc gccgatcatc
     1261 atgaaaagga aattgaccag gtaggacggg ttggcgattc cgccgatgtc gatcgcgttg
     1321 aacccggcgg tgcgcatgct gaccgaatgg aacgccgcgc ccagcacccg ctcgcccggc
     1381 gtcatcgcgc cgaaggtggc cggattgctc cactccgccg ccagcatcag cacggtgccg
     1441 ccgatcagca ggaccagcgt ggtggtgatc gtcagccggc tgtggagcga ccaggggcgc
     1501 tgccgccggg ccggatcgcg gcgggtccag atctcgtgca gcaccggaaa gccgatcccg
     1561 ccgatcacga tcgccagcat gatcggcagc aggatcgcgc cgtcgcggtg ccaggacatc
     1621 agattgtccg aaaaggtgga gaagccggcg ttgttgaagg ccgacacagc atgaaacacg
     1681 gcgtgccagg cggcttcggc cggggccatg ccgtggccga tccaccagcg cagccacagg
     1741 gagaaggcaa tgacggcctc gatcgccacg ctgaccacga agatcagctt gagcaggctg
     1801 gccgcctcgg cgcggttgag gtggccgtgc tcggtcaggg cgacggcccg gctcgacagg
     1861 ctgagccggc ggcccgccac cagccccagc agggtcgcgg cggtcatgat gccgaggccg
     1921 ccgatcttga tcatcagcag gatcgcggcc tgtccggcgc cggaccagta ggttggcgtg
     1981 tcgaccacga tcagcccggt cacgcagatc gccgaagtgg cggtgaacag cgcggtcatg
     2041 aaccctgcgc cctgtccgtc gacggtcgag aagggcagca tcagcaccag cgtgcccagc
     2101 gcaatcgcgg ccaggaatgc cagggggacg atgcgggtag ggtggaactg gcgctgcatg
     2161 gtgctccgcg ccgccaggcc cggtgatggc ggctggctgc ggctcatacc ggggcccggg
     2221 cggcttgcca aggcaagtcc ctgtcattgc agcgtcattg cgatccctcc ctgccgcgta
     2281 cccgcccgcg cggaggaaat cgtgcgccag ccgtgcgcac cccttttccc gccgcgccaa
     2341 ttcggctaac cgccgcccat gggattcttc ggcaagatct tcacctggtg gaacggggcg
     2401 accgttacca ccctgctcaa ctcggcgctg accggcgaaa aggttggcac ggatgcccag
     2461 ggcaacacct attaccgggc gaagaagccc tatcccaagg gccacccctt tgccggcaag
     2521 gaacgccgct gggtgatcta caacggcgcc aacgacgcca gccgggtccc ggccgaatgg
     2581 cacggctggc tgcacggctc gtttgaaggc gtgcccgaat cgaacctgcc cccgccgcgg
     2641 atctgggaag tggattatac ccccaatccc accggcacgc cgaacgcgca ccgtccgcag
     2701 tacagcaagg gtgggggccg caaggacggc gcttacgagg cgtggtcgcc ggacgcctga
     2761 tgcgcgctgc ggctctcctg ctgctggcgc cgctggcgct gtccgcctgc agcggcggcg
     2821 gcggagccga tcccaacgcc accgaagtgc ccaaggaagc ggccggcagt gccgtggccg
     2881 gggccgtggt ggaaagcgaa tacggcaccc cggtgaaaga ccgggtcgcc acgctgggcc
     2941 tgctcaacaa gcgcaccaac ttttcgcagg acgtggtcct gaaaaccggc gaggccaagc
     3001 ggatcggcaa tgtcacgatc aagctggcca cctgtgagcg cagcctgccg tgggaaagcc
     3061 cgcccgagac cggcgccttc gtgcaggttt tcgtcgagga acgcgcgggc agcgaccagc
     3121 cgctgcaatg gcgcaaggtc ttttccggct ggctgttcaa gaactcgccc agcctcaacg
     3181 tggtggaaca cccggtctat gacgtctggg tgaaggactg cgcgatgaag ttcccgggcg
     3241 aggaagacag cccggccgcc gcggcatcgg ccagcagcgc cgaaaagccc gccggcagcg
     3301 ccagcgcggc ccccgccgcc tcgccggccg cctcgcccac ggcgtcccct tcgcccaagc
     3361 cctcggctgc gcccaaggcg cctccggcca aggcgccccc ggccaaggcc ccgtcgccca
     3421 gcgtcgggct ttgagccgcg cgcagccggc tggcatagtc gctctggctg atctccaccg
     3481 cgcccagcga ggcgaggtga tcggtcatga actggcagtc cagcagcacg gccccggccc
     3541 ggcgcagcgc cgcgaccagc caggccagcg ccactttcga ggcatcgctg cggcggctga
     3601 acatcgattc gccgcaaaac acccggtcaa agcccacgcc atagagcccg ccgaccagct
     3661 cctccccctg ccagcattcg atcgaatggg cgtggcccag ccggtgcagt tcgcggtagg
     3721 tccgctcgat ccggtcgctg atccaggtcc cgccggggtc gcgccgcggc ccggcacatt
     3781 cggtgatcac cgcatcgaaa gcggcgttgc aggtcacgcg gaaagtcccg cgtttcagcg
     3841 cttttgccag cgaatgcgaa cagtgaaagc cgtccagcgg caagatcgcc cggcggcgcg
     3901 gctcgaccca gaaaatctcc ggatcgtcgc gcgcgtcggc catcggaaac acgccggtgc
     3961 ggtaggccag catcagcatg gccgggtcga tcggcggagg cgtggcggat ccgtgcacag
     4021 cgattcttta catggacctt tgcgttgcgc ttgtcatcgg gcagtcttgt ctctagaggc
     4081 gcatccgcct gcaggagtgt agctcagttg gtagagcatc ggtctccaaa accgagggcc
     4141 gcgggttcga atcctgccac tcctgccagc gcctctcccg ccaagcgata gaccgatgcc
     4201 cgaagccgct ccgcccgtgc cgtctccttc gccgcgcgcg ggtctgcgcc agtggctgac
     4261 ggccgagccg gggccgcgcg aatttgccgg gctggccttc gttctcgcgc tgctctccgc
     4321 cctgccggtg ctggtcgcga cctatccgca gatggtcgac tatccggccc acctggcgcg
     4381 gatgcacatc atgctgacgc gggatcaaag cccgttcctg caggaatact atggcttcga
     4441 atggcgctgg atgggcaacc tcggtgccga tctgctggtc attccgctga cctggtttgt
     4501 cagcctggaa accgccgggc ggatcatggc cgggatcatc ccgccgctga ccgcgctgtc
     4561 gctgatggcg gcggaatggg cggtacggcg gcgaatcggc atggccagcc tgctggcctt
     4621 cgccttcatc tggtcgccct cggcgattct cggcttcctc aatttcgggc tggcccaggc
     4681 cggggcgctg ttcgcctttg cgctgtgggt cctgctcgac gggaaaagct ggcgcgcgcc
     4741 gctgttcatc ccgatcggga tttttgtctg gctgggccac gtttcgggct gggggatcct
     4801 gggaatcctg gtgttcggtt acgaatggtc gaaggacaag tcgtggcgcg ccttcctggc
     4861 gccctggccg ctgttcttcc cgttcctctc gctgatcctg ggcgacaacc cgggcaagcc
     4921 gcccagctat ggcccggccc cgcagctcta caagtgggcg atctggaagc aggcgatgcg
     4981 cggcacgctg caatgggtcg actatgccat ggcgatcggc gtctgcctgc tgctggcggc
     5041 ctcgctggcc tggcgcaagt gggacggcaa gctgggctgg gcggcagcga tgatgctggt
     5101 ctcctcgctg gtcctgccgc gccacatctt tggcggagac ctggtcgacg cgcggatgat
     5161 ctcggccggg ctgatggtcg gctgcctgtg cctggcctgg cgcgcgccgc gctggattgt
     5221 cttgctcgcc ccggcgctgt tcctctcgcg cctggcctac accacgctcg actgggaacg
     5281 cgattcgcgc gagaccgccc aggcgctgac cgcgctggaa ggcctgccca agggtgccaa
     5341 ggtcgccagc tatgtggtga ccgagcgcag cgaatggggc ttcaacgggg acgagcacat
     5401 ctgcggctat gccgtggtcc ggctcgatgc cttcaccaac tgcaacttcg cgctgcccgg
     5461 catccacatg atgacgatca acggcggcgg accgttcttc cgcgatccgt tccaccgcct
     5521 gaaccaccgc gccgggctga agatcgacat ctccgactac aacccggcca agtacaccga
     5581 ctacctctgg tacgtcggcc agtccgaccc ggtgaagctg cccccgggct tcaaggtgga
     5641 gcagcgcgga cggacctggt tcctcgcccg gcagacgggc aagcggccgg acaccgaata
     5701 gggcctcccg caaaagccgc gcccgggcat cttgcaaaag cgctgcgcga tggctaagtg
     5761 cggcggtgtc atccgacata ggagaacact ttgcccctcc cggccaactt ggccggggcg
     5821 gcgatggccc ctaggcggaa gacacgagac gattcagcaa ggcgagtgag cgttccatgg
     5881 ccaagttcaa ccccggcgaa ttcatgcgcc aggtccagac cgaagcgcgc aaggtggtgt
     5941 ggcccagccg caaggaaacc accacgaccg cgatcttcgt ggc
//
LOCUS       DASUDV010000463         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1318739,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000463 DASUDV010000000
VERSION     DASUDV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318739"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..929
                     /gene="thiE"
                     /locus_tag="VFV30_10005"
     CDS             309..929
                     /gene="thiE"
                     /locus_tag="VFV30_10005"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX4848466.1"
                     /translation="MTDCQLYLISPLDVGGAFPDRLARALDAGSVAAFQFRVKGIDEH
                     EAARLAAPLQAICASREVAFIVNDSISLAKRLKADGVHLGQGDGTVAEARERLGREAQ
                     IGVTCHDSRHLAMEAGEAGADYVAFGAFFPTQTKQTDHVATPDLLEWWQALMEIPCVA
                     IGGITPENCGPLVKAGADFLAVSHAVWGGDEAEAVKAFHQAIAAAA"
     gene            1063..>2269
                     /locus_tag="VFV30_10010"
     CDS             1063..>2269
                     /locus_tag="VFV30_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX4848467.1"
                     /translation="MRELTIRGILLGAVLTVLFTAANVYLGLKIGLTFATSIPAAVIS
                     MALLKAFADSTIQENNIVQTIASAAGTLSAIIFVLPGLVMIGWWTGFPYWQSVAVIAV
                     GGILGVMYSVPLRRALVTGSDLPYPEGVAAAEVLKVGAGVGGAEENRKGLAAILWASL
                     LSAGYTLLAKMKLVAEEISTVVKFGLGHTAFSASFSLALIGVGHLVGLAVGVAMLVGL
                     VIGWLVLVPYYTFTMPPVGAELADLVSTAFRQKVRFVGAGTIGVAAIWTLLKMIGPIA
                     RGISGALAAARQRKAGQGDSLALTERDLPIGVVGGTILLAMLPIALLLADFAAGGPVM
                     AALVPTLGLSIVYVLLAGVVIASVCGYMAGLIGASNSPISGVGILASLGISLLLVAIF
                     GQGGDEASTR"
BASE COUNT          324 a          736 c          816 g          393 t
ORIGIN      
        1 gcgctccgtc cgcttgaaac cgcacgcgca agacaaaccg gccggcatga aagcaatcga
       61 ccgatgccgg gcagcggctg tcttcgacca ccaccaaagg aacgaccgag gcggtttccg
      121 acaactggac cgtttcgttg agggggcccg aacccacgaa aatccgagtg tctgaggcca
      181 gcgcggctcc ggaaacggcg agcgctgcaa tcggcaggat gaggcgtgac atggccacag
      241 attgtccgcg cgagcctgaa ccgcagccga acgcttggcg cggcgcggct ggcaggttag
      301 agggccgcat gaccgattgc cagctctacc tcatttcgcc gctcgacgtc ggcggcgcgt
      361 tccctgaccg gctggcccgc gcgcttgatg ccgggtctgt ggcggcgttt cagttccggg
      421 tgaaggggat cgacgagcac gaggctgcgc gccttgccgc gccgctgcag gcgatctgcg
      481 ccagccgcga ggtggccttc atcgtcaacg attcgatttc gctggccaag cggctcaagg
      541 ccgatggcgt gcatctgggg cagggcgacg gcaccgtggc cgaagcgcgc gagcggctgg
      601 gccgcgaggc gcagatcggc gtgacctgtc acgacagccg ccatctggcg atggaagccg
      661 gtgaggccgg ggccgactat gtcgccttcg gggccttctt cccgactcag accaagcaga
      721 ccgaccatgt cgcgacgccg gacctgctgg aatggtggca ggcgctgatg gaaatcccct
      781 gcgtcgcgat tggcgggatc acgccggaaa actgcggtcc cttggtcaag gccggggctg
      841 atttcctggc cgtttcgcac gccgtctggg gcggtgacga ggcggaagcc gtgaaggctt
      901 ttcaccaggc gatcgccgcc gcggcctgat cgcggcttgg cggcgggttt tctgcccgcc
      961 cgaactgtgc aacaccatcc gccgagggga ccttcgcggc acgcgccgcg gagccccgcc
     1021 ggcgtggcgc ccatccgggc cgcggattga gagggggctg caatgcgtga actgacgatc
     1081 cgggggatcc tgctgggggc ggtgctgacc gtcctgttta ccgcggccaa cgtctatctc
     1141 gggctcaaga tcgggctgac ctttgccacc tcgatcccgg cggcggtgat ctcgatggcg
     1201 ctgctcaagg cctttgccga cagcacgatc caggaaaaca acatcgtcca gaccatcgcc
     1261 tcggcggcgg ggacgctctc ggcgatcatc tttgtcctgc ctggcctggt catgatcggg
     1321 tggtggaccg gctttcccta ttggcaatcg gtggcggtga tcgcggtcgg cgggatcctg
     1381 ggggtgatgt attcggtgcc gctgcgccgc gcgctggtga ccggatcgga cctgccctat
     1441 cccgaaggag tcgccgcggc cgaagtgctg aaagtgggtg ccggggttgg cggggccgag
     1501 gaaaaccgca agggcctggc cgcgatcctc tgggcctcgc tgctctcggc cggctacacc
     1561 ctgctcgcca agatgaagct ggtggccgag gaaatcagca ccgtggtcaa gttcgggctg
     1621 ggccacacgg ccttttcggc cagcttctcg ctggcgctga tcggggtcgg gcacctggtt
     1681 ggcctggcgg tcggcgtggc catgctggtc gggctggtga tcggctggct ggtgctggtc
     1741 ccctattaca ccttcacgat gccgccggtc ggcgcggagc tggccgatct ggtctccacc
     1801 gcctttcgcc agaaggtccg cttcgtcggc gccgggacca tcggcgtggc ggcgatctgg
     1861 accctgctca agatgatcgg gccgatcgcc cgggggatct ccggcgccct ggccgcggcc
     1921 cggcagcgca aggccgggca gggcgacagc ctggccctga ccgagcgcga cctgccgatc
     1981 ggcgtggtgg gcggcacgat cctgctggcg atgctgccga tcgcgttgct cttggcagac
     2041 tttgccgccg gcggcccggt catggccgcg ctggtcccca cgctgggcct ctccatcgtc
     2101 tacgtgctgc tggccggggt cgtgatcgcc tcggtctgcg gctacatggc ggggctgatc
     2161 ggggcgtcga acagcccgat ttccggcgtc ggcatcctcg ccagcctggg gatctcgctg
     2221 ctgctggtgg cgatcttcgg gcagggcgga gacgaagcct caacccggg
//
LOCUS       DASUDV010000464         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1407624,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000464 DASUDV010000000
VERSION     DASUDV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407624"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFV30_10015"
     CDS             complement(<1..533)
                     /locus_tag="VFV30_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX4848468.1"
                     /translation="MRLDEFDPNAINVEDQRGGRRGLPGGRGGQVGCGTLVIALIAAA
                     VFGVDPSQMIGGLEQMQGPAQEGPALGGESVGESCRADASSLQMCNALSSLNKTWEPV
                     FAAAGIPFQAPKLVFYSQMGKSGCGAAQSAMGPFYCPADYGIYLDTDFFAQMDRELGA
                     KGKFARDYVLAHEYGHH"
     gene            622..1377
                     /locus_tag="VFV30_10020"
     CDS             622..1377
                     /locus_tag="VFV30_10020"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX4848469.1"
                     /translation="MFLSGKRALVTGSTSGIGLAVARSLAAEGAEVVLSGFGEADAIA
                     ALCAELNAAHVPADLSRRDGVEALMAGAGPIDILVNNAGMQHVAPVEEFPVDKWDQII
                     ALNLTAAFDACRLAIPHMKAAGWGRIINTASAHALTASPYKAAYVAAKHGLSGLTKTL
                     ALELATHNVTANCICPGYVWTPLVENQIPDTMAARGMTREQVINDVMLVRQPTRKFVQ
                     PSDIGEMAVFLCRDAAANITGTNISMDGGWTAE"
     gene            1487..2980
                     /locus_tag="VFV30_10025"
     CDS             1487..2980
                     /locus_tag="VFV30_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX4848470.1"
                     /translation="MKRFWQAMNRVFAPLLAAALVVQPLAAAKPNKARLELEGQLRAH
                     VTELASDAYEGREPGTEGEVKTLRYLGREWFNIGLVSGTNDPAHPWFAPVTIVAREPD
                     ASRAVFTRKGKRLFVPQEAVLALTSGKRGLVENAPVLFVGKGEAIPPRAELAGRVALL
                     LDGGIDNSERQNALLKAGASAVLTVLDGDRTLENVTAHRSRKGYALAGDKLGGDLEGF
                     ITPAALNGALVGIGLDVTKLQAQAADPAFVPRVIDLAVSLEATTRETRIKTHNLIGKL
                     PGKRPELGAVLLLAHWDHFGRCAPAPAEDQICNGAVDNASGLAVLTEVARRLAKGPQL
                     DRDVYFLATTAEEIGFLGVEAFAEDPPLPLGQIVAAFNIDTVALAPAGRPFAVVGKGM
                     TPLDPHIAAYAKSAKKKLADGDAANAYVRRQDGWALMKYDVPAVMISSSWSDIAVVER
                     FMEGDYHRPGDQLSRGIELGGAADDVEFLLGLTRWFGDAKRVPFAVK"
     gene            3068..4522
                     /gene="guaB"
                     /locus_tag="VFV30_10030"
     CDS             3068..4522
                     /gene="guaB"
                     /locus_tag="VFV30_10030"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX4848471.1"
                     /translation="MDIPLGLTYDDVLLRPAESDILPSMADTRTQLTREIALNIPVIS
                     SAMDTVTEADMAIVMAQLGGIGVLHRNLTVEEQCAAVRAVKRFESGMVVNPITISPDA
                     TLGEARALMEAHRISGIPVVEAGGKLVGILTNRDVRFAANPAQPVRELMTHENLATVR
                     PGVSQEEARRLLHQRRIEKLLVVDENYHCTGLITVKDMEKAITYPDATKDATGRLRVA
                     AATTVGDKGFERTEALLAAECDVVIIDTAHGHNRDVARAVERVKKISNSAQVIAGNVA
                     TYEATRALIDAGADGVKVGIGPGSICTTRVVAGVGVPQLTAVMEAARAARESGVPIIA
                     DGGLRTSGDAAKALAGGASTIMVGSMLAGTDEAPGETFLYQGRAYKSYRGMGSVGAMG
                     RGSADRYFQGDIKDQMKLVPEGIEGQVPYKGPAKDVIHQLVGGIKAAMGYTGSATIED
                     LRTRGQFVRITNAGLKESHVHDVTITREAPNYKL"
BASE COUNT          781 a         1509 c         1568 g          706 t
ORIGIN      
        1 atgtggtggc cgtattcgtg agccagcacg taatcgcgcg cgaacttgcc cttggcgccc
       61 agctcgcggt ccatctgggc gaagaaatcg gtgtcgaggt agatgccgta gtcggccggg
      121 caatagaatg gccccatcgc gctttgcgcc gcgccgcagc ccgatttgcc catctgcgaa
      181 tagaacacca gcttgggcgc ctggaacggt attccggccg ccgcgaagac cggttcccag
      241 gtcttgttga gcgaggagag ggcgttgcac atctgcaggc tgctggcatc ggcgcggcag
      301 ctttcgccca cgctttcgcc gcccagggcc gggccttcct gcgccggacc ctgcatctgt
      361 tccagcccgc cgatcatctg gctgggatcg accccgaaca ccgccgcggc gatcagcgcg
      421 atcaccaggg tgccgcagcc gacctgtccg ccgcgcccgc ctggcaggcc gcgccggccc
      481 ccgcgctggt cttccacgtt gatcgcgttg ggatcgaatt catcaagacg catcggcgga
      541 ccctccaagc ggtctggaca aagccgggtc cagccgcaat ggccacgcta taccgcaacg
      601 gccacgtgtt tggagtcgag aatgttcctt tccggaaagc gcgcacttgt aacgggttcc
      661 acttcgggga tcggcctggc ggtcgcccgt tcgctggcgg cggagggggc cgaagtggtg
      721 ctctccggtt ttggcgaggc cgatgcaatc gccgcgctct gtgcggagct gaacgcggcc
      781 catgtgccgg ccgatctctc gcgccgcgac ggggtggaag cgctgatggc cggggccggg
      841 ccgattgaca tcctggtcaa caacgccggg atgcagcacg tcgcaccggt cgaggaattc
      901 ccggtcgaca agtgggacca gatcatcgcg ctcaacctga cggccgcctt tgacgcttgc
      961 cggctggcga tcccgcacat gaaggcggcc gggtgggggc ggatcatcaa caccgccagc
     1021 gcccacgcgc tgaccgcttc gccctacaag gcggcctatg tcgcggccaa gcacgggctt
     1081 tcggggctga ccaagacgct ggcgctggaa ctggcgaccc acaacgtgac cgccaattgc
     1141 atttgcccgg gctatgtctg gacgccgctg gtggaaaacc agatccccga taccatggcg
     1201 gcgcgcggga tgacgcggga acaggtgatc aacgatgtga tgctggtgcg ccagccgacc
     1261 cgcaagttcg ttcagccttc ggacatcggc gagatggctg tattcctgtg ccgtgacgct
     1321 gccgccaaca tcaccggaac caacatctcg atggatgggg gctggaccgc cgaataggcg
     1381 gcgcccggcg acgcgcactg taacattcct gccacccttg gccgcaatcg acgaaccgag
     1441 tgcggtcccg cttgccgcct tgcgccattg acgggataag ggggcgatga agcgtttctg
     1501 gcaagcgatg aaccgcgtgt tcgccccgct gcttgcagcg gcgctggtcg ttcagccgct
     1561 cgccgcggcc aaaccgaaca aggcgcggct ggaactggaa ggccagctgc gtgcccatgt
     1621 gaccgaactg gccagcgatg cctatgaagg gcgcgagccc gggaccgagg gcgaggtcaa
     1681 gaccctgcgc tatctgggcc gcgaatggtt caacatcggg ctggtttccg gcaccaacga
     1741 tccggcccat ccgtggtttg cccccgtgac catcgtcgcg cgcgagcccg atgccagccg
     1801 cgcggtcttc acccgcaagg gcaagcgcct gttcgtcccg caagaggcgg tgctggcgct
     1861 gacgtcgggc aagcgcggcc tggtggagaa cgcgcctgtg ctgttcgtcg gcaagggcga
     1921 ggcaattccg ccgcgggcgg aactggccgg ccgggtcgcc ctgctgctgg acggcgggat
     1981 cgacaattcg gagcggcaga acgcgctgct gaaagccgga gcctcggccg tgctgaccgt
     2041 gctggacggc gatcgcacgc ttgagaacgt caccgcgcac cgcagccgca agggctatgc
     2101 cctggccggc gacaagctgg gcggggatct ggagggcttc atcaccccgg cggcgctgaa
     2161 cggcgcgctg gtcgggatcg ggctcgatgt gaccaagctt caagcccagg cggccgatcc
     2221 ggcctttgtg ccgcgggtga tcgaccttgc cgtctcgctg gaggcgacca cccgcgaaac
     2281 ccggatcaag acccacaacc tgatcggcaa gctgcccggc aagcgcccgg aactgggggc
     2341 cgtgctgctg ctggcccact gggaccattt tggccgctgc gccccggctc cggccgagga
     2401 ccagatctgc aacggcgcgg tcgacaacgc cagcggcctg gccgtgctga ccgaagtggc
     2461 gcggcggctg gccaaggggc cgcagctgga ccgcgatgtc tatttcctgg ccaccacggc
     2521 cgaggagatc ggcttcctgg gggtggaggc ctttgccgag gatccgccgc tgccgctggg
     2581 gcagatcgtg gccgccttca acatcgatac cgtggccctg gccccggccg gacggccctt
     2641 tgccgtggtc ggcaagggga tgaccccgct cgatccgcac atcgccgcct atgccaagtc
     2701 ggcgaagaag aagctggccg atggcgatgc cgcaaacgcc tatgtccgcc gccaggatgg
     2761 ctgggcgctg atgaagtacg atgtgccggc ggtgatgatc tccagctcgt ggtcggacat
     2821 cgcggtggtc gaacgcttca tggaagggga ctaccaccgc ccgggcgacc agctcagccg
     2881 cggaatcgag ctgggcgggg cggccgatga cgtcgaattt ctgctcggcc tgacccgctg
     2941 gttcggcgat gccaagcggg tgcctttcgc ggtgaaatag ggcactagcc cttttccggg
     3001 cccgcgttta catgggccgc cacgaatcga tccgcgcagg ctttgcgcaa acagggacag
     3061 tggcaccatg gatatccctc tcggcctgac ttacgacgac gtcctgctgc ggccggccga
     3121 gtccgacatc cttccttcga tggccgatac ccgcacccaa ttgacgcgcg aaatcgcgct
     3181 caacattccg gtcatctcct cggcgatgga caccgtgacc gaggccgaca tggcgatcgt
     3241 catggcccag ctcggcggga tcggggtgct ccaccgcaac ctgacggtcg aggaacagtg
     3301 cgccgcggtc cgcgcggtga agcggtttga aagcggcatg gtggtcaacc cgatcacgat
     3361 cagccccgac gccaccctgg gcgaagcgcg cgcgctgatg gaagcgcacc gcatttccgg
     3421 gattccggtg gtggaggccg gcggcaagct ggttggcatc ctgaccaacc gcgatgtgcg
     3481 ctttgccgcc aacccggccc agccggtgcg cgaattgatg acgcacgaga acctggccac
     3541 ggtgcgcccc ggcgtcagcc aggaagaggc gcggcggctg ctgcaccagc ggcggatcga
     3601 aaagctgctg gtggtggacg agaattacca ctgcaccggc ctgatcaccg tcaaggacat
     3661 ggaaaaggcg atcacctatc ccgacgcgac caaggacgcg accgggcgcc tgcgcgttgc
     3721 ggcggcgacc acggtgggtg acaagggctt tgaacggacc gaagcgctgc tcgccgccga
     3781 atgcgacgtg gtgatcatcg acactgccca cggccacaac cgcgatgtcg cccgcgcggt
     3841 cgaacgggtg aagaagattt ccaactcggc ccaggtgatt gccggcaatg tcgccaccta
     3901 tgaagccacc cgcgcgctga tcgatgccgg ggcggacggg gtcaaggtcg ggatcgggcc
     3961 gggctcgatc tgcaccaccc gcgtggtcgc gggggtgggc gtgccgcagc tgaccgcagt
     4021 gatggaagcg gcccgcgccg cgcgtgagag cggcgtgccg atcatcgccg acggcggcct
     4081 gcggaccagc ggcgacgcgg ccaaggcgct ggccggcggg gccagcacga tcatggtcgg
     4141 ctcgatgctg gccgggacgg acgaggcccc cggtgagacc ttcctctacc agggccgcgc
     4201 ctacaaatcg taccgcggga tgggctcggt tggggccatg ggccgcggca gcgcggaccg
     4261 ctatttccag ggcgacatca aggaccagat gaagctggtg cccgaaggca tcgagggcca
     4321 ggtgccctac aagggaccgg ccaaggacgt gatccaccag ctggtcggcg gcatcaaggc
     4381 ggcaatgggc tataccggca gcgccacgat cgaggatctg cggacccgcg gccagttcgt
     4441 ccgcatcacc aacgccgggc tcaaggaaag ccacgtccac gacgtgacga tcacccgcga
     4501 ggctccgaac tacaagctgt gacgccccag gcgcgcgtcc aggccgggat cgagattctc
     4561 gatc
//
LOCUS       DASUDV010000465         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_435248,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000465 DASUDV010000000
VERSION     DASUDV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435248"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..2180)
                     /locus_tag="VFV30_10035"
     CDS             complement(90..2180)
                     /locus_tag="VFV30_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX4848472.1"
                     /translation="MARSDIRRSAPPAGRPGKPAKPKPTGFRLFLRRLFVWGTAVGLL
                     AVLALAVAVGLTAQTLPTFDQIKTTQSEQMIVVRARDGTQLVSLGPSYGKWVSHDEIP
                     QVMKDAMLSVEDRRYYNHIGIDPVGLARSFWVRFQEGRWKQGGSTITQQLARTVFLNN
                     SRTFARKLREAVLALALEWTFSKDQILELYLNKVYFGGGAYGVDSASRKFFGHPATDL
                     SLPEAAIIAGLVKAPSHYAPTADADAAVGRGSVVLGLMVENEKVSAAEAGKVDFKKIV
                     FAKEAGQNSVRYFTDWALPQLDLVLGETDQPIEVWTTLDVNLQRAATEAIQRNVPGGA
                     QGALVSMDRDGAVLALVGGTDYVTSNYNRATNAVRQPGSAWKLFVYLSALEAGYTPNA
                     EVIDEPVTIEGWSPRNSGGGFAGKIDVRTAFAYSKNTVAAQLGQEVGFSSVAAMAKRF
                     GISTPISTVPSMVLGTNEVRLIDMTRAFASISAKGASVEPYGILKVVTTDGKVLYQRQ
                     APKANQLVPAHVAAGITDMLQSAVSVGTGKAAQIGRPVAGKTGTTTANKDGWFLGFSS
                     GVTTGVWMGRDDDRPVPGLQGGTAPARAFASYMRVAVANRPVERFATDATLPAWQLES
                     EDELLMSGAPEDYQYVDESGNLIAPERPREDPVPSNNGGLVPVTPPPAANEDFLNEAT
                     GGGTKPAAAAPPGE"
     gene            2369..2767
                     /locus_tag="VFV30_10040"
     CDS             2369..2767
                     /locus_tag="VFV30_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848473.1"
                     /translation="MQSRRKGAVRYIVLAMVLQVLSRSLERMGDLAVAAAGPGQGAGS
                     PYYIPALLLTLTAIALFLLALKRLFDRITGRDGGSATPVYDPVEETVSAPAGFDPDEA
                     LRRYMAKRPAETQPAATTPGRPGGFGRRGL"
     gene            complement(2787..3224)
                     /locus_tag="VFV30_10045"
     CDS             complement(2787..3224)
                     /locus_tag="VFV30_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX4848474.1"
                     /translation="MLGIEAHWVWLGLGLLLAIGEMTIPGVFLIWMAGAALVTGLLSW
                     FVPIGIPFQVLLFAVLSIVFVFSGRRWLRQHPIEAADPKMNDRGARAVGEVVVVSQVI
                     ETGQGRVKLGDSEWLARGPDAEPGTRMRVTGSDGAVLLVEHLH"
     gene            complement(3230..>4043)
                     /locus_tag="VFV30_10050"
     CDS             complement(3230..>4043)
                     /locus_tag="VFV30_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4848475.1"
                     /translation="YTLAAEPGLHVIIPFIDRVGRKVNMMEQVLDIPGQEIITKDNAM
                     VGVDAVVFFQVLDAGKAAYEVANLYVAIMQLTTTNLRTVMGSMDLDETLSKRDEINGK
                     LLVVIDHATAPWGVKITRIEIKDIRPPADISNAMARQMKAEREKRAQILEAEGMRAAE
                     ILRAEGEKQGAILQAEGRREAAFRDAEAREREAEAEAKATTMVSEAIASSGTQALNYF
                     IAQKYTDAVSQFATSPNAKTILFPVEATQLIGTLGGVGELVKDALGEREARK"
BASE COUNT          620 a         1383 c         1355 g          685 t
ORIGIN      
        1 cagatcctgc gtcccggcca gcccggcctg gtctacatcg ccatccggct gagctgaggc
       61 gagcggtccg gcctagccgg aaccgatggc tattcccccg gcggggcggc ggcggccggc
      121 ttggtgcctc cgcccgttgc ctcgttgagg aaatcctcgt tcgcggccgg cggcggtgtc
      181 accggcacca gcccgccgtt gtttgacggc accggatcct cgcgcgggcg ttccggcgcg
      241 atcaggttgc cggattcgtc gacgtactgg taatcctcgg gcgcgccgct catcagcagt
      301 tcgtcctcgg attccagctg ccaggccggc agggtggcgt cggtggcaaa gcgctcgacc
      361 gggcggttgg caaccgcgac ccgcatgtag ctggcaaagg cgcgggccgg ggcggtcccg
      421 ccctgcaggc ccggcaccgg ccggtcatcg tcgcggccca tccacacacc ggtcgtgacc
      481 ccgctggaga agcccaggaa ccagccgtcc ttgttggccg tggtggtgcc ggtcttgccg
      541 gccaccggac ggccgatctg ggccgccttg ccagtgccga cgctgaccgc gctttgcagc
      601 atgtcggtga tcccggcggc gacatgggcc ggcaccagct ggttcgcctt gggggcctgg
      661 cgctggtaca gcaccttccc gtctgtggtg acgaccttga ggatcccgta aggctcaacc
      721 gaggcgccct tggccgagat cgaggcgaag gcgcgggtca tgtcgatcag ccgcacctcg
      781 ttggtgccca gcaccatcga cgggacggtg gagatcggcg tcgagatccc gaaccgcttg
      841 gccatggccg cgaccgagga gaacccgacc tcctgtccca gctgcgccgc gacggtgttc
      901 ttcgaatagg caaaggctgt gcgcacgtcg atcttgccgg caaagccgcc gcccgaattg
      961 cgcgggctcc agccctcgat cgtgaccggt tcgtcgatca cttcggcgtt gggggtatag
     1021 ccggcttcca gcgccgagag atagacgaac agcttccagg ccgaacccgg ctggcgcacc
     1081 gcgttggtcg cgcggttgta gttcgaagtg acatagtcgg tcccgccgac aagggcgagc
     1141 accgcgccgt cgcggtccat gctgaccagc gcgccctggg cgccgcccgg cacgttgcgc
     1201 tggatcgcct cggtcgcggc gcgctgcagg ttcacgtcca gcgtggtcca gacctcgatc
     1261 ggctggtcgg tctcgcccag caccagatcg agctgcggca gcgcccagtc ggtgaagtag
     1321 cgcaccgaat tctgcccggc ttccttggcg aagacgatct tcttgaaatc gaccttgccg
     1381 gcttcggcgg cgctgacctt ttcgttctcg accatcaggc ccagcaccac cgaaccgcgg
     1441 cccaccgcgg cgtcggcatc ggcggtcggc gcatagtgcg acggggcctt gaccaggccg
     1501 gcgatgatcg cggcttcggg caggctcagg tcggtcgccg gatggccgaa gaacttgcgg
     1561 ctggctgaat cgacgccgta agccccgccc ccgaaataga ccttgttgag atagagctcg
     1621 agaatctgat ccttggaaaa ggtccattcc agcgccagcg ccagcacggc ttcgcgcagc
     1681 ttgcgggcaa aggtccgcga attgttgagg aacaccgtgc gggccagctg ctgggtgatg
     1741 gtcgatccgc cctgcttcca gcgcccttcc tggaaccgca cccagaacga gcgggccagg
     1801 cccaccgggt cgatcccgat gtggttgtag taccgccggt cctcgaccga gagcatggcg
     1861 tctttcatca cctgcgggat ctcgtcgtgg ctgacccact tgccgtaact cggcccgagc
     1921 gagaccagct gggtcccgtc gcgggcgcgg accacgatca tctgttcgct ttgggtggtc
     1981 ttgatctggt caaacgtcgg cagggtctgc gcggtcaggc caaccgccac ggccagcgcc
     2041 aacacggcta gcaggcccac cgcggtgccc cacacgaaca ggcggcgcag gaacagccgg
     2101 aaaccggtcg gcttcggctt ggcgggcttg cccggtcggc cggcaggcgg cgcgctgcgc
     2161 cggatatcgg aacgtgccat agggctagat caatcctccc gaagggcaag catgcccgtc
     2221 cacccggccc caataggcca attggcgcgc agcgttaatc cgggaaatgt gaagatcgcg
     2281 tgcaggcgcc acctgtggtg acgaattggc aacccggggc gggctatgct gcgcggattg
     2341 gaaggtcctg gcgaaggagg ccaagccgat gcaaagccgc aggaaaggcg ccgtgcgcta
     2401 tatcgtgctg gccatggtgc tgcaggttct gtcgcgctcg ctggagcgca tgggcgacct
     2461 tgccgtggcc gcggccgggc cgggccaggg ggccggatcg ccctattaca ttcccgccct
     2521 gctgctgacc ctgaccgcga ttgccctgtt cctgctggcg ctcaagcggc tgtttgaccg
     2581 gatcaccggc cgggacggcg gatccgcgac gccggtctac gatccggtcg aagagacggt
     2641 ctccgcacct gccgggttcg atccggacga ggcgctgcgg cgctatatgg ccaagcgccc
     2701 ggcagagacc cagccggcgg cgaccacccc ggggcggccg ggcggatttg gccgccgcgg
     2761 cctctgaacc accggggcct gcggcctcag tgcagatgct cgaccagcag gaccgcgccg
     2821 tctgatccgg tcacccgcat ccgggtgccc ggttcggcat cggggccgcg ggccagccat
     2881 tcgctgtcgc ccagcttgac ccggccctgc ccggtttcga tcacctggct gaccaccacc
     2941 acttcgccca ccgcgcgggc gccacgatcg ttcatcttcg gatcggccgc ctcgatcggg
     3001 tgctggcgca gccagcgccg gccggaaaag acgaagacga ttgacaggac cgcaaacagc
     3061 aggacctgaa aggggatgcc gatcggcacg aaccagctga gcagcccggt gaccagcgcc
     3121 gcgccggcca tccagatcag gaacacgccc ggaatggtca tttccccgat tgccagcagc
     3181 aggcccagcc ccagccagac ccagtgcgct tcgatgccca gcatcgcgct cacttgcggg
     3241 cctcccgctc acccagcgcg tccttgacca gttcaccgac gccgccaagc gtgccgatca
     3301 gctgggtcgc ctcaaccggg aacaggatgg tcttggcatt gggcgaggtc gcgaactggc
     3361 tcaccgcgtc ggtgtacttc tgggcgatga agtagttcag cgcctgggtg cccgacgagg
     3421 caatcgcctc ggagaccatg gtcgtcgcct tggcttcggc ctcggcctcg cgctcgcggg
     3481 cttcggcgtc gcggaaagcg gcttcgcggc ggccctcggc ctggaggatc gcgccctgct
     3541 tttcgccctc ggcgcgcagg atctcggcgg cacgcatgcc ttcggcctcg aggatctggg
     3601 cgcgtttctc gcgctcggcc ttcatctggc gggccatggc gttggaaatg tcggccggcg
     3661 gacggatgtc cttgatttcg atgcgggtga tcttcacccc ccacggcgcg gtggcgtgat
     3721 cgatcaccac cagcagcttg ccgttgatct cgtcacgctt ggacagcgtc tcgtcgaggt
     3781 ccatcgagcc catcaccgtg cgcaggttgg tggtggtgag ctgcatgatc gcgacataga
     3841 ggttggccac ctcgtaagcg gccttgccgg catcgagcac ctggaagaac accactgcgt
     3901 ccacgccgac catggcgttg tccttggtga tgatttcctg gcccggaatg tcgagcacct
     3961 gttccatcat gttcaccttg cggcccaccc ggtcgatgaa cgggatgatc acgtgcaggc
     4021 ccggttcggc cgccagcgtg tac
//
LOCUS       DASUDV010000466         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1584246,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000466 DASUDV010000000
VERSION     DASUDV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584246"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..721
                     /locus_tag="VFV30_10055"
     CDS             233..721
                     /locus_tag="VFV30_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848476.1"
                     /translation="MITHRDLESTLLALQPALDLARYPWWILGSAAVVLHGADPDEIW
                     DVDVLLDPRDCAAVFARLGLTPKPGQADSRFRSAVFHRWNQASLPVELFAGFCLFEEG
                     EWHEFVPQTRIAATIGRARAFVPEREELIALLQRFGRPKDQRRIAALSRSGPFPSRSE
                     NA"
     gene            complement(678..893)
                     /locus_tag="VFV30_10060"
     CDS             complement(678..893)
                     /locus_tag="VFV30_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX4848477.1"
                     /translation="MRTEPTIRIPLGVLGLFVALMVYGGLVARYIAPLIADWHALLQT
                     PVYILLGVVWLLPLKRFLIWMETGRFG"
     gene            complement(893..1465)
                     /locus_tag="VFV30_10065"
     CDS             complement(893..1465)
                     /locus_tag="VFV30_10065"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX4848478.1"
                     /translation="MIEKKQLRAELKRRRAEHVAAIPDFQRALLFRRPPEPILALIPE
                     GAVISVFHEMTGEVPASNYGRWFYERGHRIALPWLESRGAPMQFREWANPFLEDGLVA
                     DPYGAKQPPADAKSLVPTVMFCPLLGFTSTGGRIGYGAGFYDRWLADNPPQLAIGLAW
                     DCQLEEGLPLEAHDVPLHAVITPTRLYGPF"
     gene            complement(1692..1988)
                     /locus_tag="VFV30_10070"
     CDS             complement(1692..1988)
                     /locus_tag="VFV30_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX4848479.1"
                     /translation="MSNVTLQIGGRAYTVACAEGEEAHVAGLGRAIDAKVQSIGTGHN
                     EIRQLLFAALLLADELHELKGRAAPAPAPAGPDPAATLEAIADRLEKCAAALEG"
     gene            complement(1985..2182)
                     /locus_tag="VFV30_10075"
     CDS             complement(1985..2182)
                     /locus_tag="VFV30_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848480.1"
                     /translation="MDEDRSSRALARIEGALARIEAAARRPAASGDAALQELQARHDR
                     LRGAVQESLEQLDQLIEGTQG"
     gene            2352..>2980
                     /locus_tag="VFV30_10080"
     CDS             2352..>2980
                     /locus_tag="VFV30_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX4848481.1"
                     /translation="MSLDSPRLAPMANAIRALSMDAVQAANSGHPGMPMGMADVATVL
                     YSQFLKFDPAAPKWPDRDRFVLSAGHGSMLIYSLLHLTGYAAPSMDDIRNFRQLHSPC
                     AGHPENTELDGVECTTGPLGQGLAMAVGMAMAERHLNAVHGDDLVSHRTWVIAGDGCL
                     MEGVNHEAVGLAGTLKLGQLNVLWDDNNITIDGDTALSTCEDIPARYRA"
BASE COUNT          491 a          992 c         1009 g          488 t
ORIGIN      
        1 acatagacgt gaagcccgtc ctccagcttg gccgactggt ccaccccgac attcttgtcg
       61 agcgcggcct ggaacgcccg ctcggccagc cagccccggc cgaacacagc aagcagcccc
      121 acggcggcaa tcacgacggc aaggatcagc agcagtttgc gcttcacggc aggtctctcc
      181 ggttgtttgg ccgcaggttg cctctgcggc ggccctgcgg caagcgggaa ctgtgattac
      241 ccaccgcgac ctcgaatcga cgctcctggc gcttcagccg gcgctggacc tggcccggta
      301 cccctggtgg atcctgggca gcgccgcggt ggtgctgcac ggggccgatc cggacgagat
      361 ctgggatgtc gacgtgctgc tggacccgcg cgattgtgca gcggtgtttg cccggctggg
      421 gctgacgcca aagccggggc aggccgacag ccggttccgc tccgccgtgt tccaccgctg
      481 gaaccaggcc tcgctcccgg tagagctgtt cgccgggttc tgcctgttcg aggagggcga
      541 gtggcatgaa ttcgtgccgc agacgcggat cgcggccacg atcggcaggg cgcgggcctt
      601 tgtgcccgag cgggaggaac tgatcgccct gttgcagcgc tttggccggc cgaaggacca
      661 gcggcggatt gccgcgctca gccgaagcgg cccgtttcca tccagatcag aaaacgcttg
      721 agcggcagca accagaccac gcccagcagg atatagaccg gggtctggag cagggcgtgc
      781 caatcggcga tcagcggcgc gatatagcgc gccaccagcc cgccatagac catcagcgcg
      841 acaaacaggc cgagcacgcc aagcgggatc cggatcgtgg gttcggtgcg catcagaaag
      901 gtccgtaaag gcgagtgggg gtgatcacgg cgtgcagcgg cacatcatgg gcttccagcg
      961 gcaggccttc ctccaactgg cagtcccagg caagcccgat cgccaactgc ggcggattgt
     1021 cggccagcca gcggtcgtaa aagccggcgc catagccgat ccgcccgccc gtgctggtaa
     1081 agcccagcag cgggcagaac atcaccgtgg ggaccaggct tttggcatcg gccggcggct
     1141 gcttcgcgcc ataggggtcg gcgaccagcc cgtcctccag gaacgggttg gcccattcgc
     1201 gaaactgcat cggcgcgccg cggctttcca gccagggtag ggcgatgcgg tgcccgcgct
     1261 catagaacca gcggccatag ttggacgcgg gcacttcgcc ggtcatttcg tggaacaccg
     1321 agatgactgc gccctccggg atcagggcca agatcggctc gggcggacgg cgaaacagca
     1381 atgcgcgctg gaaatcgggg atggcggcga cgtgttcagc ccggcggcgt ttcaattcgg
     1441 cgcgtagctg ctttttctcg atcacgcgcg gctccgtggg gcgggaaggt gggtgacggg
     1501 accaccatgg gccgttgccg ggaaatcctc tgacgcgttc acgtcaggtg ggaaccatat
     1561 gtgccagacc ccaatggaaa cagagtggtc tgaccaggga cagcccccat ttggattgct
     1621 tatagcctca gggatgttct gtcggttcgt accgggcagt acccgtcagc ccctagatag
     1681 gcgttgcggc ctcacccctc aagggcagcg gcgcattttt cgagccggtc ggcaatcgct
     1741 tcaagggttg cggccggatc ggggccggcc ggggccgggg ccggggcagc gcggcctttc
     1801 agctcgtgca gctcgtcggc cagcagcagc gcggcgaaca gcagctggcg aatctcgttg
     1861 tggccggtgc cgatcgactg gaccttggca tcgatcgccc ggcccagccc agcaacgtgg
     1921 gcttcctcgc cttcggcgca ggcgacggta taggcgcggc cgccgatctg aagggtgacg
     1981 ttgctcatcc ctgggttcct tcgatcagct gatcaagctg ctcgagcgat tcctgcaccg
     2041 ctccacgcag gcggtcgtgg cgggcttgca actcttgcaa tgcggcatcg ccggatgcgg
     2101 ccgggcggcg cgcggcggcc tcgatccggg ccagggcgcc ttcgatccgc gcgagcgcgc
     2161 ggctgctgcg gtcttcgtcc attccatcac gctaccgcaa tgtcatgcac cggcaaagtg
     2221 ttgcagggcg gatttccccc ggtttgagaa gcccggcggg cggggctgcc ttgacggggg
     2281 gccatgcctt cgcaagagag gcgacgcgcc gaatcgccgc cgcattcctg ccctttcagg
     2341 agcccttgcc gatgagcctc gattcccccc gcctcgcgcc catggccaat gccattcgcg
     2401 cgctttcgat ggacgcggtt caggcggcca attcgggcca ccccggcatg ccgatgggca
     2461 tggccgacgt ggcgaccgtc ctgtacagcc agttcctgaa gttcgatccc gccgcgccga
     2521 agtggccgga ccgggaccgc ttcgtcctgt cggccgggca cggctcgatg ctgatctatt
     2581 cgctgctgca cctgaccggt tatgccgcgc cgtcgatgga cgacatccgg aatttccgcc
     2641 agctgcacag cccctgcgcc gggcacccgg aaaacaccga gctggacggc gtggaatgca
     2701 ccaccggccc gctggggcag ggcctggcca tggctgtcgg catggccatg gccgaacgcc
     2761 acctcaacgc ggtgcacggg gatgatctgg tcagccaccg cacctgggtg atcgcgggcg
     2821 atggctgcct gatggaaggc gtcaaccacg aagcggtcgg cctggccggc acgctcaagc
     2881 tcggccagct caacgtgctg tgggacgaca acaatatcac catcgacggc gacaccgccc
     2941 tgtcgacctg tgaagacatt cccgcccgct accgcgccag
//
LOCUS       DASUDV010000467         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_413425,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000467 DASUDV010000000
VERSION     DASUDV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413425"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFV30_10085"
     CDS             complement(<1..731)
                     /locus_tag="VFV30_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4848482.1"
                     /translation="MARTVSTDVLVIGGGTAGFAAAHAAASSGARVRLLEGTSKIGGV
                     MAFCPGMPWGGGFPVGRTIGGLFETLTEQLRQMQPPMAQIRPSTLENFGAEVIYDHEA
                     AVFAMFQMLEDVGVDLHLGTYASAPVMDGNRLRAVECFDRTGPFTIETGMVIDCSGDG
                     DISAKAGVPYTLGDGRGNMMGVTLSFTMLDALWDEVFASDDPYFTAYAAEGIAEGRLH
                     PDLAKIYLMRGFHPDTVFCNSVHIRG"
     gene            complement(737..2311)
                     /locus_tag="VFV30_10090"
     CDS             complement(737..2311)
                     /locus_tag="VFV30_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX4848483.1"
                     /translation="MADWSDDIDTGTYGRSTRSFAGMLVDPLVFFPTAVISLAVIAYS
                     LIDPDGSATLFSGIRSAATTNFDWFFMSAGNLLLLFCVAVAVSPLGRIRLGGKGATPD
                     YSRLSWFSMLFGAGMGIGLVFYGVSEPVAHFTSAMAGGAGAPLGGAAGDPAAARDLAM
                     AATIFDWALHPWAMFAVTGLALALFAYDYGMPLSMRSAFYPLFGKAVWGRLGDAIEVL
                     AVFATIFGLATSLGLGAQQAMAGITFLYHISSSPASILGLIAVMAGITFLSVRGGIDR
                     GIRILSEANMLVALGLLIFVLLTGPAMALLSDLFRNIAVYLQNLPALSNPIGREDTGF
                     YRDWSVYYWAWWISWAPFVGMFMARISLGRTVREFIAGAMIAPSLLGILWLTVFGDAS
                     IAAIVAGKSGGIQDASIDTQLFVLLGTLPWAQVTSFVAICLILIFFVTGWDSGTLVID
                     TMTSGGRTDTPLRQKAFWLFAVGGIGVMLLLTGGLTSLQAGSIATGLPLGLVLLFMCW
                     GTLRGLLAIHRRGKTG"
     gene            complement(2428..3282)
                     /gene="folD"
                     /locus_tag="VFV30_10095"
     CDS             complement(2428..3282)
                     /gene="folD"
                     /locus_tag="VFV30_10095"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX4848484.1"
                     /translation="MRADVIDGRKLAREVTDAVARETAGLARRPGLAVIIVGDDPASH
                     LYVRNKVRTAEKVGFKSETIRLPADTSEADVLGRVAWLNGREDIDGILVQLPLPPQLN
                     TLRVMAAVDPGKDVDGLHALNAGRLTQGSQALIPCTPMGCLKIIKSVIPDLTGCHAVV
                     IGASNIVGKPMAALLLEENATVTQTHIHTRDTQFICRGADILVSAVGKPGLVRGDWIK
                     PRAVVIDVGTTRVEQPDGTVKTMGDVAFEEAAEVAGAITPVPGGVGPMTIACLMENTL
                     IAARNRGG"
     gene            complement(3296..>3832)
                     /locus_tag="VFV30_10100"
     CDS             complement(3296..>3832)
                     /locus_tag="VFV30_10100"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEX4848485.1"
                     /translation="RLGVPFRSAGGFNASKVPDAQAAYESANTLQQGMLAGVNFMLHT
                     AGWLEGGLAMGYEKFVMDCDQAAMMAIYAAGVDMSENGQALDAMREVGPGQHFLGCSH
                     TQANFKTAFYRSTIADNNSYEQWVEDGKLDAEQRAEKLYKEMLANYVAPELDPEIDAR
                     LLAFMAEKKASFPDSNIS"
BASE COUNT          699 a         1321 c         1220 g          592 t
ORIGIN      
        1 ccgcggatgt ggaccgaatt gcagaacacc gtatcggggt gaaagcctcg catcagatag
       61 atcttggcaa gatcgggatg cagccggccc tcggcgatgc cctctgcggc gtaagcggtg
      121 aaatagggat catcgctcgc gaagacctca tcccacagcg cgtcgagcat ggtgaaggac
      181 agggtcaccc ccatcatgtt cccgcgtccg tcccccaggg tatagggcac ccccgccttg
      241 gccgaaatgt cgccatcccc cgagcagtcg atcaccatcc cggtttcgat cgtgaagggc
      301 ccggtccggt caaagcattc gaccgcgcgc agccggttgc cgtccatcac cggggcgctg
      361 gcataggttc ccaggtgcag gtcgacgccc acatcttcca gcatctgaaa catcgcaaag
      421 accgccgctt cgtgatcgta gatcacctcg gcgccgaagt tttccagcgt actggggcgg
      481 atctgggcca tcggtggctg catttgccgc agctgctcgg tcagggtttc gaacagcccg
      541 ccgatggtcc ggccgaccgg gaaccccccg ccccacggca tgccggggca aaaggccatc
      601 accccgccga tcttgctggt tccctccagc agccggaccc gcgccccgct gcttgccgcg
      661 gcatgggccg ccgcaaaacc ggcggtgccg ccaccgatca ccagcacgtc ggtggaaacg
      721 gtacgggcca tccggctcag ccggtcttgc cgcgccggtg gatcgccagc aggccgcgca
      781 gcgttcccca gcacatgaag agcaggacca gccccagcgg caggcccgtg gcgatcgacc
      841 cggcctgaag cgaggtcagg ccgccggtca gcagcagcat caccccgatc ccgccgaccg
      901 cgaacagcca aaaggccttc tgccgcagcg gcgtgtcggt gcggccgccc gatgtcatcg
      961 tgtcgatcac cagcgtgccc gaatcccagc ccgtcacgaa gaagatcagg atcaggcaga
     1021 tcgcgacgaa cgaggtaacc tgcgcccagg gcaaggtgcc gagcaggacg aacagctggg
     1081 tgtcgatcga tgcgtcctga atgccgccgg acttgcccgc gacgatcgcg gcaatgctgg
     1141 catcgccaaa cacggtcagc cacaggatgc cgagcaggct gggcgcgatc atcgccccgg
     1201 cgatgaattc ccgcacggtc cggcccagcg agatccgggc catgaacatg cccacgaacg
     1261 gcgcccagct gatccaccag gcccagtaat agaccgacca atcgcggtaa aagccggtat
     1321 cctcacgccc gatcgggttg gacagggccg gcaggttctg caggtaaacc gcgatgttgc
     1381 ggaacaggtc cgaaagcagc gccatggccg ggccggtcag caggacaaag atcagcaggc
     1441 ccagcgccac cagcatgttg gcttcgctga ggatccggat cccccggtca atcccgccgc
     1501 gcaccgacag gaacgtgatc cccgccatga ccgcgatcag ccccaggatc gaggcggggg
     1561 aactgctgat gtggtacagg aacgtgatcc ccgccatcgc ctgctgcgcg ccaaggccaa
     1621 gcgaggtggc aaggccgaag atcgtcgcga agaccgccag cacctcgatc gcgtcgccca
     1681 gccggcccca cacggccttg ccgaacagcg gatagaaggc cgagcgcatc gacagcggca
     1741 tgccatagtc ataggcgaac agcgccagcg cgaggccggt taccgcaaac atcgcccagg
     1801 ggtgcagcgc ccagtcgaaa atggtcgcgg ccatggccag atcgcgcgcg gcggcgggat
     1861 cgcccgcggc tccgccgagc ggtgccccgg ccccgcccgc cattgcggag gtgaaatggg
     1921 cgaccggttc ggacaccccg taaaagacca gaccaatccc catccctgcg ccgaacagca
     1981 tcgagaacca ggagagccgc gaatagtccg gcgtcgcgcc cttcccgccc agccggatcc
     2041 ggcccagcgg cgagaccgcc acggccacgc agaacagcag cagcaggttg ccggccgaca
     2101 tgaagaacca gtcgaaattg gtggtcgcgg ccgagcggat gccggaaaac agcgtcgccg
     2161 acccgtccgg atcgatcagg ctgtaggcaa tgaccgccag cgagatcacc gcggtgggaa
     2221 agaacaccag cggatcaacc agcatccccg caaagctgcg cgtggaccgg ccgtaagtgc
     2281 cggtatcgat atcgtcagac cagtccgcca tggctttgcc ctctccctgg acaaagcttg
     2341 gagcatggac cgggcaaggt ccagtccatc gccagcatta atgggcagca tcactcccgg
     2401 catcaccggc agcgggcggc agggcattca gccgccgcgg ttgcgggccg cgatcagggt
     2461 gttttccatc aggcaggcga tggtcatcgg gccgaccccg ccgggcactg gagtgatcgc
     2521 cccggccact tctgccgctt cctcgaacgc gacgtcgccc atggtcttca cggtcccgtc
     2581 cggctgctcg acccgggtcg tgccgacatc gatcaccacg gcgcgcggct tgatccagtc
     2641 gccccggacc agccccggct tgcccaccgc actgaccagg atgtcggccc cgcggcagat
     2701 gaactgggtg tcgcgggtgt ggatgtgggt ctgggtgacg gtggcgttct cttccagcag
     2761 cagcgccgcc attggcttgc cgacgatatt cgacgcgccg atcaccacgg cgtggcaccc
     2821 ggtcagatcg ggaattaccg acttgatgat cttgaggcag cccatcggcg tgcacgggat
     2881 cagcgcctgg ctgccctggg tgagccgccc ggcgttgagc gcgtggagcc cgtccacgtc
     2941 cttgcccgga tcgaccgcgg ccatcacgcg cagcgtgttg agctgcgggg gcagcggcaa
     3001 ctgcaccagg atcccatcga tatcctcgcg gccgttgagc caggcgaccc gtccaagcac
     3061 atcggcttcg ctggtgtcgg ccggcaggcg gatcgtttcg gacttgaacc cgaccttctc
     3121 ggccgtgcgc accttgttgc gcacatagag atggctggct ggatcgtcac ctacgatgat
     3181 caccgccagc ccgggcctgc gcgccaggcc ggcggtttcc cgcgcgacag catcggtcac
     3241 ttcgcgggcg agcttgcgcc cgtcgatcac atcggctcgc attgtctgcc cggtcctagc
     3301 tgatgttcga atccgggaag ctggccttct tctcggccat gaacgccagc aggcgcgcgt
     3361 cgatctcggg atcgagttcg ggcgcgacat agttggcgag catctccttg tagagcttct
     3421 cggcccgctg ttcggcgtca agcttgccgt cctcgaccca ctgctcatag ctgttgttgt
     3481 cggcaatggt tgaccggtag aacgcggtct tgaagttggc ctgggtgtgg ctgcagccca
     3541 ggaagtgctg ccccggacca acttcgcgca tggcgtccag cgcctggccg ttctcgctca
     3601 tgtcgacgcc ggcggcatag atcgccatca tcgccgcctg atcgcagtcc atcacgaact
     3661 tttcatagcc catggcgaga ccgccttcga gccagccggc ggtgtggagc atgaagttga
     3721 ccccggccag catgccctgc tgcagcgtgt tggcgctttc ataggcggcc tgggcatcgg
     3781 gcaccttgga ggcgttgaag ccgcccgcgc tgcggaacgg cacgccaagc cg
//
LOCUS       DASUDV010000468         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_457920,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000468 DASUDV010000000
VERSION     DASUDV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457920"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFV30_10105"
     CDS             <1..250
                     /locus_tag="VFV30_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542431.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HEX4848486.1"
                     /translation="LLDMATAWQPVEGSDEEEFVGISRATGARVWRATRADLVFGSNS
                     QLRAVAEAYAEAGMEAKFVADFVKAWTKVMEADRFDLA"
     gene            319..714
                     /locus_tag="VFV30_10110"
     CDS             319..714
                     /locus_tag="VFV30_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4848487.1"
                     /translation="MKGFVGDIEGLTLANTDFRRVLYTAREMQLVLMALQPGEEIGEE
                     VHDDVSQFFRFEAGEGEVWIDGACHRVKGDDAVIVPAGARHNVKAIGSAPLRLYTIYS
                     PPEHVDGTVHRTRADALAAHEHWDGKTSE"
     gene            complement(767..2056)
                     /locus_tag="VFV30_10115"
     CDS             complement(767..2056)
                     /locus_tag="VFV30_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4848488.1"
                     /translation="MPGEQSLPAFRFLPHQPSLWRLLRVGIGDVGSCMPAAILDQFAV
                     QLPGPGAPLIVSSPDLAREVLNDRTELFARDRFIRRLFRRAWGQGLAGAEGPAWERQR
                     KAATPFFRPSAVAAQLAAFARESDRVAGTLGEDQTVELNWLAMRIVSRIVFSVLVDAR
                     GAIDPEEVARDVPGYITRIVDFGLPDLLPLPEPVLDWLSGINRDFRARRVRAAADRLA
                     AMRGGTEPYRDLIALLERAGPARDNIGGLIPAAMDTTVHGLSWALNTLALRPEWQERL
                     AREARAAGPQPTLDQLPLIRRAVQEVLRLYAPAPMLARSAAVDQEIAGHKVRRGQTVI
                     VAIYAMHRHSRLWDDPDGFDPDRFLPERGPHPASMPFGTGPRMCIAAQFAQAEIAVVL
                     ARLLARFEVRPTGHQPDIGLRTATSSRNGLMVRMRAR"
BASE COUNT          382 a          747 c          811 g          364 t
ORIGIN      
        1 cctgctcgac atggccactg cctggcagcc ggtcgagggc agtgatgaag aggaattcgt
       61 cgggatcagc cgtgccaccg gcgcccgggt ctggcgggcc acccgcgccg atctggtgtt
      121 cggctccaac tcgcaactgc gcgcggttgc cgaagcctat gccgaggcgg gcatggaggc
      181 gaaattcgtt gccgatttcg tcaaggcctg gaccaaggtg atggaggccg accgcttcga
      241 tctggcttga tcgaggtcat ggaagatcgt gcctgccggg ggcagtctgc tctggcaggc
      301 acgatcagga gaatgacgat gaagggattt gtcggcgata tcgaaggtct taccctggcg
      361 aataccgact ttcgccgggt gctctacacc gcgcgtgaaa tgcagctggt gctgatggcg
      421 cttcagccgg gcgaggaaat cggcgaggaa gtccacgacg atgtcagcca gttcttccgc
      481 ttcgaggcgg gtgagggcga ggtctggatc gatggcgcct gccatcgggt caagggtgat
      541 gatgcggtga tcgtgccggc cggcgcgcgc cacaacgtca aggcaatcgg atcggcgccg
      601 ctccggctct acacgatcta ttcgccgccc gaacatgtcg atggcacggt ccaccgcacc
      661 agggccgatg ccctggccgc gcatgagcac tgggacggca agaccagcga gtagtcggaa
      721 ggtcggcgct caccgcgtgg tgcgcggaac ggcgtccgac cgatcgctag cgcgcccgca
      781 ttcgcaccat caggccattg cgcgagctgg ttgcggtgcg caggccgatg tcgggctggt
      841 gcccggtcgg ccgcacttcg aaccgggcca gcagccgggc cagcactacg gcaatctcgg
      901 cctgcgcgaa ctgggcggcg atgcacatcc ggggacccgt accaaacggc atcgaggcgg
      961 gatgcgggcc ccgttcgggc aggaaccggt ccggatcgaa gccatcggga tcgtcccaca
     1021 gccgcgaatg gcggtgcatg gcatagatcg ccacgatcac cgtctgcccg cgccgcacct
     1081 tgtgtccggc gatttcctga tccactgccg cgctgcgcgc cagcatcggc gccggggcat
     1141 agagccgcag cacttcctgg accgcgcggc ggatcaaagg cagctggtcc agcgtcggct
     1201 gcggaccggc agcgcgcgct tcgcgggcca gccgctcctg ccattcgggt cgtagcgcca
     1261 gggtgttgag cgcccaggac aagccgtgga cggtggtgtc catcgccgcc gggatcagcc
     1321 cgccgatatt gtcgcgcgcc ggcccggcgc gttccagcag ggcaatcagg tcccggtagg
     1381 gttcggtgcc gccgcgcatg gcggccaggc ggtcggccgc ggcgcgcacc cgccgcgccc
     1441 ggaaatcgcg attgatcccg ctcagccagt cgagcactgg ttcgggcagg ggcagcaagt
     1501 cgggcaggcc gaaatcgacg atccgcgtga tatagccggg tacatcgcgg gcaacctctt
     1561 cgggatcgat cgccccgcgc gcgtcgacca gcaccgaaaa gacgattcgt gaaacgatcc
     1621 gcatggccag ccagttgagc tcgactgtct ggtcttcgcc cagggtgcct gccacgcgat
     1681 cgctctcgcg ggcgaaagcg gccagttgcg cggcgacggc gctcgggcgg aaaaacggag
     1741 tcgcggcctt gcgctggcgc tcccacgccg ggccttcggc gccggccagc ccctgacccc
     1801 aggcgcggcg gaacagccgg cggatgaacc tgtcgcgcgc gaacagctcg gtccggtcgt
     1861 taagcacttc gcgggccagg tcgggtgaag agacgatcag cggcgcaccc gggccgggca
     1921 actggacggc gaattgatcg aggatcgccg ctggcatgca ggatccgaca tcgccgatcc
     1981 caacgcgcag cagccgccac aggctgggct ggtgcggcag gaaacggaac gctggaagtg
     2041 attgttcccc gggcatcggc ggttctccga tcgctgcgca ggctagcctg tccgcgtggc
     2101 ttgcgccatt gcaaacggcg caggcgcagc tgccctttgc gggccggcca agcagctttc
     2161 ctacagcccg gcgggcatgt cacaatggca caagtgctgg aacgaatgcc tcttgaaccg
     2221 cgccgtcatg acgcaccgca ggggtctgag gtgagccctg gctggcgcca gattttttgt
     2281 tcgcagggcg tgcgttttcg tccg
//
LOCUS       DASUDV010000469         9221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1783431,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000469 DASUDV010000000
VERSION     DASUDV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783431"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..717)
                     /locus_tag="VFV30_10120"
     CDS             complement(64..717)
                     /locus_tag="VFV30_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX4848489.1"
                     /translation="MSEPSSLADLRLLLAPALADAAAFDGWSETAVRDAAAAKGIDPD
                     AAVFAFSGGAMEMIEAWVRSVDAAMAEALPAERIGNLKIRERIRTLVQFRLDAIAHQK
                     EALRRALAIMAMPQNAGRALALGWKSADAMWRLAGDTATDYNHYTKRLTLGSIYAATL
                     AVYVNDTSEDHADTRAFLDRRIEGIMKFEKAKARLLRPREEMFSPVRLLGRLRYPAR"
     gene            complement(726..1352)
                     /locus_tag="VFV30_10125"
     CDS             complement(726..1352)
                     /locus_tag="VFV30_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4848490.1"
                     /translation="MSNFRKLSLALAVAVPLAGALALPAPALAQFSDSYKFLEAVKKK
                     EGDTVTDLLANGSPNLINTRDVTSGESALHIVTTRRDLTWMRFLIAKGANVNIRDARG
                     VTPLVIACNLNFVEGVEVLIENGARVDESNNSGETPLITAVHNRNVPLMRLLLKAGAD
                     PDRADNSGRSARDYARLTNAPTLLTVIETDAKPKPKPGQGPQTYGPKF"
     gene            1449..2072
                     /locus_tag="VFV30_10130"
     CDS             1449..2072
                     /locus_tag="VFV30_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4848491.1"
                     /translation="MTKPNLRAALRLATLGLATLTLAACLPSGKDAEPPAITGASIEG
                     STIGGPFTLYDKDGKPRTWDSFKGKYRIVYFGYTFCPDACPMDVSVMIQGFTKFAKDH
                     PDLAAQVQPIFITVDPARDTPARVGEFTAAFSPSLLGLTGSPAEVEVAVKAFKVASAR
                     GADTPGGYLMDHSRMAYLMGRNGEPIAALPVDLNPDAVASDLAKLVK"
     gene            2069..2527
                     /locus_tag="VFV30_10135"
     CDS             2069..2527
                     /locus_tag="VFV30_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX4848492.1"
                     /translation="MNAGRDPFWTKPIEALSRAEWEALCDGCGQCCLHKAEDEDTGDV
                     YMTNVACRLLDIPSARCSDYRNRKAQVPDCVRLTPRLAATLSWLPETCAYRLRADGLP
                     LPDWHYLVCGDPDAVHAAGISVRGKAIPEALAGPLEQHVILPPGVELADD"
     gene            2520..3245
                     /locus_tag="VFV30_10140"
     CDS             2520..3245
                     /locus_tag="VFV30_10140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX4848493.1"
                     /translation="MIDWLLKGGREDPQVEVGGRLIPVVLRRTAQAKRMTMRLAPDGS
                     EVRITLPSWGRSAEALDFARKRSGWLAAQLAAVPEPVSLGHGAVLPYLGMQRTIVHSP
                     DGSRRVRVEDDVLHVGGPAESLAPRLARWLQGEARRLLAEDLDHYCALAAQPRPRLML
                     SSARRRWGSCAHDGTIRINWRLVMAPGYVRRSVVAHEVAHLVHFDHSPAFHALLDSLF
                     EHDIRAANRWLKREGRSLYLPLG"
     gene            3301..3753
                     /locus_tag="VFV30_10145"
     CDS             3301..3753
                     /locus_tag="VFV30_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848494.1"
                     /translation="MSYWQKINPGGAVSDLATVFRDAGPKRWPIALLSAAITVGVFSI
                     MAGESWKKPRPLPEVTYITSWPTDRTDAETRAFIEENQKRKDAEEAAAAKADADAREL
                     WATLGKVSGMDVEKMKQQAEAERAAEAAKKKAETDALLKQVKVEPVEK"
     gene            3743..4708
                     /gene="ribD"
                     /locus_tag="VFV30_10150"
     CDS             3743..4708
                     /gene="ribD"
                     /locus_tag="VFV30_10150"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX4848495.1"
                     /translation="MKSKPDAAWLAAAARLASRGIPLSRPNPAVGAILVKDGRVIARG
                     WTQPGGRPHAEAIALAAAGDAAQGATLYVTLEPCAHQSPRGPACADLVAASGVERVVI
                     GCPDPDPRTAGAGAARLRAAGIAVEEAGDPACAASLAGYLMQRRHGRPHVTLKLALSE
                     DGHLAPPPGEGQWLTGEVARAHVHAMRARMDAILVGRGTLEADAPRLDVRLPGIADRS
                     PARWLLTRGPAPAGWQALPAPEAIGGMDGAQYLMVEGGAGAARAFLSAGLVDRLLLYR
                     APRTVGGSGPALPELTTAALADNPAWHLADTRQLGNDTVQTYEAL"
     gene            4708..5325
                     /locus_tag="VFV30_10155"
     CDS             4708..5325
                     /locus_tag="VFV30_10155"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX4848496.1"
                     /translation="MFTGIVTAIGTIRDVSERGDLRAVIACPYDPAAIAIGASIACSG
                     VCLTVVERGGSAGDAWFAVDVSGETVSKTAQDQWRQGRKLNLEQALKLGDELGGHIVT
                     GHVDAVGRIALREEVGGSLHLAVTTPAEIAPFIAPKGSVTVDGVSLTVNAVDDHADGT
                     CTFHLNIIPHTAEVTTLGGLKAGDGVNLEIDVLARYLQRMQSLRG"
     gene            5332..5775
                     /locus_tag="VFV30_10160"
     CDS             5332..5775
                     /locus_tag="VFV30_10160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848497.1"
                     /translation="MATLRRLSLAHAGQTAALHRSSGALIPGYDTSLHTPAEDLDFHR
                     RIVLVENTVWGLFEEEELAGFIAYQPGWVDHLYVRPDRLRCGIGSHLLTFAQRQQDEL
                     RLYTFQSNARGRAFYEHHGFVIEEMTDGARNEEQMPDITYLWRAG"
     gene            complement(5772..6320)
                     /locus_tag="VFV30_10165"
     CDS             complement(5772..6320)
                     /locus_tag="VFV30_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848498.1"
                     /translation="MLNRQPVLEGPRLLLRPLVAGDWDALFAVASDPEIWAIHPQPNR
                     WQEPVFRRFFADAMERGGALAVIDKASGAVIGSSQYKQDDPADGGVVEIGWSFLARSH
                     WGGATNREMKRLMLEHALAQVARVEFRVGESNLRSRRAMEKIGGRLTGRSEMVDTPTV
                     PMRHVIYEITRESFAAGPLAQA"
     gene            complement(6320..7495)
                     /locus_tag="VFV30_10170"
     CDS             complement(6320..7495)
                     /locus_tag="VFV30_10170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX4848499.1"
                     /translation="MLSNLEQQPADALLALIKLHAADTRTDKIDLGVGVYRTGQGATP
                     VFGAIKAAEQKLIDTQDSKAYLGPEGDMGFVHALMPYVFGKDDPTMGGRIEGMQAPGG
                     TGAVRLAVALAKKAGVQRVWMGVPSWPNHAQIFADLGMELKTFAHAKGGAADMDALRG
                     AIAQAQPGDAILLHGCCHNPTGIDYTNAEWDEIAPLLAPAGLLPILDLAYQGLGAGME
                     EDAYGVRRVLAEVPEALIAYSCDKNFGMYRDRVGAIYVMAENAEVLNRALSNGHALAR
                     AAWSMPPDHGGAAVRLILEDAGLTALWLDELDTMRTRMRQVRARLAEAGQQGGVDFAA
                     LGSQNGLFSILPLSGEQIQKLRTDHGIYMAGSGRINVAGLTMDNIDKFIADVAAVSA"
     gene            7584..8027
                     /locus_tag="VFV30_10175"
     CDS             7584..8027
                     /locus_tag="VFV30_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX4848500.1"
                     /translation="MLAPTTRPDLPVSLTAAMLRGAKGTCPRCAAPGLFHRFLKPVSH
                     CGACHQDWSHHQADDFPPYVSIFLTGHLVAPVLIALGMSDTLSLGVKLAIAMGLSSVL
                     MLLFLQPAKGAILALQWWLGMHGFTPAGRDEALGPPKAAPGSPWG"
     gene            complement(8156..8917)
                     /locus_tag="VFV30_10180"
     CDS             complement(8156..8917)
                     /locus_tag="VFV30_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX4848501.1"
                     /translation="MIVGMPRRVRGALFSLFGAVALAASVSAPAQVQPVDPDRAIDGD
                     LTPPQGTPSPAPTIVPTPPADSAAVPGETVTTPDTGPAETPAGAVPENPGTYQRDDVL
                     GAAESVFGKGAAGLAGLIEDLLRKQGEPNAYITGREGGGALVVGLRYGSGTLFHKIEG
                     QRPVYWTGPSVGFDAGANAASTFVLVYNLYNTEDIFKRYAAGEGQAYLVGGFNVSYLR
                     RGDVVLIPIRMGVGLRLGVNAGYMKFSRKQNWFPF"
     gene            9129..9221
                     /locus_tag="VFV30_10185"
     tRNA            9129..9221
                     /locus_tag="VFV30_10185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9163..9165,aa:Ser,seq:gct)
BASE COUNT         1406 a         3256 c         3116 g         1443 t
ORIGIN      
        1 ttgggccggg ggagcgggcc ggtggtgcca aggacaaggc ggatgccttg tccgcaccaa
       61 ccattaccgc gccggatagc gcagccgccc cagcaggcgg accgggctga acatttcctc
      121 gcgcgggcgc agcaggcggg ccttggcctt ttcgaacttc atgatccctt cgatccgccg
      181 gtccaggaag gcgcgggtgt cggcgtgatc ctcgctggtg tcgttgacat agaccgccag
      241 ggtcgcggca tagatcgaac cgagcgtcag ccgcttggtg tagtggttgt aatcggtcgc
      301 cgtatcgccg gcgagccgcc acatcgcatc ggcgctcttc cagcccagcg ccagggcgcg
      361 gccggcgttc tgcggcatgg ccatgatcgc cagggcgcgg cgcagcgctt ccttctggtg
      421 cgcgatcgcg tccagccgga actggaccag ggtgcggatc cgttcgcgaa tcttgagatt
      481 gccgatccgt tcggccggca gggcttcggc catggcggca tcgaccgagc ggacccaggc
      541 ctcgatcatt tccatcgccc cgccggaaaa ggcgaagacg gcggcatcgg gatcgatccc
      601 tttcgcggcg gcggcatcgc gcacggcggt ctcgctccat ccgtcaaagg ccgccgcatc
      661 ggccagagcc ggggcaagca gcagccgaag gtctgccagg ctggacggtt cgctcatcgc
      721 cgggctcaga acttggggcc gtaggtctgc gggccctggc cgggtttcgg cttgggcttg
      781 gcgtcggttt cgatcaccgt cagcagggtc ggggcgttgg tcaggcgggc atagtcgcgc
      841 gccgagcggc cggaattgtc ggcccggtcg ggatcggcgc cggccttcag cagcagccgc
      901 atcagcggca cgttgcggtt gtggaccgcg gtgatcagcg gggtttcgcc cgaattgttg
      961 ctttcatcga cccgcgcgcc gttctcgatc agcacctcaa ccccttcgac gaagttgagg
     1021 ttgcaggcga tcaccagcgg cgtgaccccg cgcgcgtcgc ggatgttcac gttggcaccc
     1081 ttggcgatca gaaagcgcat ccaggtcagg tcgcggcggg tggtgacgat gtgcagcgcc
     1141 gattcgccgc tggtcacgtc gcgggtgttg atcaggttgg ggctgccgtt ggcgagcagg
     1201 tcggtgaccg tgtccccttc cttcttcttc accgcttcca ggaacttgta gctgtcggaa
     1261 aactgcgcga gcgccggggc cggcagcgcg agtgcgcccg ccagcggcac cgcgacggcc
     1321 agagccagcg acagcttgcg gaaattggac accgaatcga tcctcctgtt gcgttgtccc
     1381 gggaatcgca cgccgggccg aaacgctcct tgcgcggcgc ggtttagcag agcatgaacc
     1441 gccgcgccat gaccaagcca aaccttcgcg ccgcccttcg tcttgccact ctgggtcttg
     1501 ccaccctgac cctcgccgcc tgcctgcctt cgggcaagga tgccgagccg cccgccatca
     1561 ccggcgcctc gatcgaggga tcgaccatcg gcgggccgtt cacgctttat gacaaggacg
     1621 gcaagccgcg cacctgggat tcgttcaagg gcaagtaccg gatcgtctat ttcggctaca
     1681 ccttctgccc ggatgcctgc ccgatggacg tctcagtgat gatccagggc ttcaccaagt
     1741 tcgccaagga ccaccccgat ctggccgcgc aggtccagcc gatcttcatc accgtcgatc
     1801 ccgcgcgcga cacgccggcc cgggtgggtg agttcaccgc cgccttttcg cccagcctgc
     1861 tggggctgac cggatccccg gccgaggttg aagtggcggt caaggcgttc aaggtcgcct
     1921 cggcccgggg cgcggatacg cccggcggct atctgatgga ccacagccgg atggcctatc
     1981 tgatgggccg caacggcgaa ccgatcgcgg cccttccggt cgatctcaac ccggacgcgg
     2041 ttgcttcgga cctggccaag ctggtcaagt gaacgccggg cgcgatccct tctggaccaa
     2101 gccgatcgag gccctgagcc gcgccgaatg ggaagccctg tgcgacggct gcggccagtg
     2161 ctgcctgcac aaggccgagg acgaggacac cggcgacgtc tatatgacca acgtcgcctg
     2221 ccggctgctc gacattccca gcgcccggtg cagcgattac cggaaccgca aggcgcaggt
     2281 gcccgattgc gtgcggctga ccccgcggct ggccgcgacg ctcagctggc tgcccgaaac
     2341 ctgcgcctac cgtctgcggg cagacggcct gcccctgccc gactggcact atctggtctg
     2401 cggcgatccc gatgcggttc acgccgccgg catatcggtg cggggcaagg ccattcccga
     2461 agcactggcc gggccgctgg aacagcacgt gatcctgccc cccggggtgg agctggccga
     2521 tgattgattg gttgctgaaa ggcggccgcg aggatccgca ggtcgaggtc ggcgggcggc
     2581 tgatcccggt cgtcttgcgc cgcaccgccc aggccaagcg gatgaccatg cgcctggccc
     2641 ccgatggcag cgaagtgcgg atcaccctgc ccagctgggg ccgcagcgcc gaagcgctgg
     2701 acttcgcccg caagcgcagc ggctggctgg ccgcgcagct ggcggcggtg cccgagccgg
     2761 tcagcctggg ccacggcgcg gtgctgccct acctcggcat gcagcggacc atcgtccaca
     2821 gcccggacgg cagccgccgg gtgcgggtgg aggacgatgt gctgcatgtc ggcgggccgg
     2881 ccgaatcgct cgctccgcgg ctcgcgcgct ggttgcaggg cgaggcgcgg cggctgcttg
     2941 ccgaagacct ggaccattac tgtgcgctgg ccgcccagcc ccggccgcgc ctgatgctga
     3001 gcagcgcccg gcggcgctgg ggctcctgcg cgcacgacgg gacgatccgg atcaactggc
     3061 ggctggtgat ggccccgggc tatgtccgcc gctcggtggt ggcgcacgag gtcgcccacc
     3121 tggtccattt cgatcacagc ccggcgttcc acgccctgct cgattcgctg ttcgaacacg
     3181 atatccgcgc cgccaaccgc tggctcaagc gcgaggggcg cagcctttac ctgccgctgg
     3241 gctgaaccgg cccgcaactc ggcactggcg caaagccgcg ccccgcccta tcttggcgcc
     3301 atgtcctatt ggcaaaagat caatcccggc ggcgccgtat ccgacctggc caccgtgttc
     3361 cgcgatgccg gccccaagcg ctggccaatc gccttgctgt ctgcggcaat cacggtcggg
     3421 gtcttttcga tcatggccgg cgaaagctgg aagaagcccc gccccctgcc cgaagtgacc
     3481 tatatcacct cgtggccgac cgaccgcacc gacgccgaaa cccgcgcctt catcgaggaa
     3541 aaccagaagc gcaaggacgc cgaggaggcc gccgccgcca aggccgatgc cgacgcgcgc
     3601 gagctgtggg ccacgctggg caaggtttcc ggcatggacg tcgagaagat gaagcagcag
     3661 gccgaagccg agcgcgccgc ggaagccgcg aagaagaagg cggagaccga tgccctgctc
     3721 aagcaggtca aggtcgagcc ggttgaaaag taagcccgac gccgcctggc tggccgcggc
     3781 ggcgcggctg gcatcgcgcg gaatcccgct gagccgcccc aaccccgctg tcggcgcgat
     3841 cctggtcaag gacggccggg tcatcgcccg cggctggacc cagcccggcg gccgccccca
     3901 tgccgaagcc atcgcgctcg ccgcggcggg cgacgcagcc cagggggcaa ccctttatgt
     3961 cacgctggaa ccctgcgccc accagagccc gcgcggcccg gcctgtgccg atctggtcgc
     4021 ggccagcggg gtggagcggg tggtgatcgg ctgccccgat cccgatcccc gcaccgccgg
     4081 agccggggcc gcgcggctgc gcgccgccgg gatcgcagtg gaggaggccg gcgacccggc
     4141 ctgcgccgcc agcctggccg gatacctgat gcagcgccgc cacggccgcc cgcacgtcac
     4201 gctcaagctc gcgctgtccg aggacggcca tctcgccccg ccgccgggcg aaggccagtg
     4261 gctgaccggc gaggtggccc gcgcccatgt ccatgccatg cgggcgcgga tggacgcgat
     4321 cctggtcggg cgcggcacgc tggaggccga tgccccccgg ctcgacgtgc ggctgccggg
     4381 gatcgcggac cgcagtccgg cgcgctggct gctgacccgc gggccagcgc ccgcgggctg
     4441 gcaggccctg cccgcgcccg aagccattgg cggtatggac ggggcgcaat acctgatggt
     4501 cgaaggcggt gcgggtgccg cccgtgcgtt cctgtctgcc gggctggtcg atcgcctgct
     4561 gctctaccgc gccccgcgca ccgtcggcgg atccggcccg gccctacccg aactgaccac
     4621 ggccgcgctg gctgacaacc cggcctggca ccttgcggat acgcgccagc ttggtaacga
     4681 caccgtgcaa acctacgagg ccctctgatg tttaccggaa tcgtcaccgc catcggcacc
     4741 atccgcgacg tgagcgagcg gggcgatctg cgcgccgtca tcgcctgccc ctatgatccc
     4801 gccgctatcg cgatcggggc ctcgatcgcc tgttcgggtg tctgcctgac ggtggtggaa
     4861 cgcggcggca gcgccggcga tgcctggttc gcggtcgatg tttcgggcga aaccgtcagc
     4921 aagaccgcgc aggaccagtg gcgccagggc cgcaagctga atctggagca ggcgctgaag
     4981 ctgggcgacg aactgggcgg gcatatcgtg accggccatg tcgacgcggt cggccggatc
     5041 gccctgcgcg aagaggtcgg cggatcgctg cacctggccg tcaccacccc ggccgagatc
     5101 gctccgttca tcgcccccaa gggctcggtc acggtcgacg gcgtctcgct gacggtcaac
     5161 gcggtggacg atcacgccga cggcacctgc accttccacc tcaacatcat cccccacacc
     5221 gccgaagtca ccacgctggg cggcctcaag gccggcgacg gggtgaacct cgagatcgac
     5281 gtgctggccc gctatctgca gcggatgcaa agcctgcggg gctgacggga agtggccacg
     5341 ctccggcggc tctccctcgc gcacgccggg caaaccgccg cgctgcaccg gtcgagcgga
     5401 gcgctgatcc cgggctatga caccagcctg cacaccccgg ccgaagacct ggactttcac
     5461 cgccggatcg tcctggtcga aaacaccgtc tggggcctgt tcgaggaaga ggagctggcc
     5521 gggttcatcg cctatcagcc gggctgggtc gatcacctct atgtccggcc ggaccggctg
     5581 cgctgcggga tcggcagcca cctcttgacc ttcgcccagc gccagcagga cgaattgcgg
     5641 ctttacacct tccagtcaaa cgcccggggc cgcgcctttt acgagcatca cggcttcgtg
     5701 atcgaagaga tgaccgacgg cgcgcgcaac gaggaacaga tgcccgacat cacctatctg
     5761 tggcgcgccg gctaggcctg agccagcggc cccgcggcga aactttcgcg ggtgatttca
     5821 tagatcacgt ggcgcatcgg aacggtcggc gtgtcgacca tctcgctgcg cccggtcagc
     5881 cgcccgccga tcttttccat cgcccggcgc gagcgcaggt tgctctcccc gacccggaat
     5941 tcgacccgcg cgacctgggc caaggcgtgc tccagcatca gccgcttcat ttcgcggttg
     6001 gttgccccgc cccagtggct gcgcgccaga aaggaccagc cgatttcgac cacgccgcca
     6061 tcggcgggat cgtcctgctt gtactgcgag gatccgatca cggcgccgct ggccttgtcg
     6121 atcaccgcca gggccccgcc gcgctccatg gcatcggcaa agaaccggcg gaagaccggt
     6181 tcctgccagc ggttcggctg gggatggatc gcccagatct cgggatcgct ggcaacggca
     6241 aacagcgcgt cccagtcgcc cgcgaccagg ggcctcagca gcagccgcgg gccttccagc
     6301 accggctgcc ggttcagcat caggcgctga ccgccgcgac gtcggcgatg aacttgtcga
     6361 tgttgtccat ggtcaggccg gccacgttga tccggcccga accggccatg tagatcccgt
     6421 gatcggtgcg cagcttctgg atctgctcgc ccgacagcgg caggatcgag aacaggccgt
     6481 tctggctgcc cagcgcggcg aaatcgaccc cgccctgctg cccggcttcg gccagccggg
     6541 cgcggacctg gcgcatgcgg gtgcgcatgg tgtccagttc gtccagccac agcgcggtca
     6601 ggcccgcgtc ttccaggatc aggcgcaccg ccgcgccgcc gtgatcgggc ggcatcgacc
     6661 aggccgcgcg ggccagggcg tggccattcg acagggcccg gttcagcact tcggcgtttt
     6721 cggccatgac atagatcgcg ccgacccggt cgcggtacat cccgaaattc ttgtcgcagg
     6781 aataggcgat cagcgcttcg ggcacttcgg ccaggacgcg gcgcacgcca taggcgtctt
     6841 cctccatccc ggcgcccagg ccctgatagg ccaggtccag gatcggcagc agcccggccg
     6901 gggcaagcag cggggcgatc tcgtcccatt cggcgttggt gtaatcgatg ccggtcgggt
     6961 tgtggcagca gccgtgcagc aggatcgcgt cgcccggctg ggcctgggca atcgcaccgc
     7021 gtagcgcgtc catgtcggcg gctccgccct tggcgtgggc aaaagtcttc agttccatgc
     7081 ccaggtcggc aaagatctgc gcgtggttgg gccagctcgg cacgcccatc cagacccgct
     7141 ggaccccggc cttcttggcc agagccacgg ccagccgcac cgcgccggtg ccgccggggg
     7201 cctgcatccc ttcgatccgc ccgcccatgg tcggatcgtc cttaccgaag acatagggca
     7261 tcagcgcatg gacaaaaccc atgtcgccct cgggcccgag gtaggccttg gaatcctggg
     7321 tatcgatcag cttctgctcg gccgccttga tcgctccgaa caccggagtc gcgccctggc
     7381 cggtgcggta gacgccgacg ccaaggtcga tcttgtccgt ccgggtatcg gcggcgtgaa
     7441 gcttgatcag cgcgagcagc gcatcggcgg gctgttgttc cagattgctg agcatgatgg
     7501 ccgggccctt agccccgcgg cttggcccgg ggcaagcaca gaatcccgct tgatcacagc
     7561 cgcgcaaggg ccgaaagggg ggcatgctcg cgcccaccac ccgtcccgac ctgcccgtca
     7621 gccttaccgc cgccatgctg cgcggggcca agggcacctg cccgcgctgc gccgcgccgg
     7681 ggctgtttca ccgcttcttg aagccggtga gccattgcgg ggcctgccac caggactggt
     7741 cgcaccacca ggccgacgat tttccgccct atgtctcgat cttcctgacc ggccacctgg
     7801 tcgcgccggt gctgatcgcg ttgggcatgt cggacacgct ttcgctgggg gtcaagctgg
     7861 cgatcgccat gggcctgtcc agcgtgctga tgctgctgtt cctgcagccg gccaagggcg
     7921 cgattctggc gctgcaatgg tggctgggca tgcacggctt cacgccggcc ggccgcgacg
     7981 aggcactcgg cccgcccaag gccgcaccgg gcagcccctg gggctaaccg gttcatccga
     8041 tctagtcagc accggcccgc ttccgcggcc cagacacaca ccgaatagac taccgggtgg
     8101 ctgggccggg ggagcgggcc ggtgatgcgc cgccgtcagg cggcaaacag aacccctaga
     8161 acgggaacca gttttgcttg cgggaaaact tcatgtagcc cgcgttgacg cccagccgca
     8221 ggcccacgcc catccggatc gggatcagca ccacgtcgcc gcggcgcagg taggacacgt
     8281 tgaaaccgcc aaccagatag gcctgccctt cgcccgcggc atagcgcttg aagatgtcct
     8341 cggtgttgta gaggttatag accagcacga aggtgctggc ggcgttcgcg ccggcatcga
     8401 agccgaccga cggcccggtc cagtaaaccg ggcgctgtcc ttcgatcttg tggaacaatg
     8461 tgcccgagcc atagcgcagc cctacgacca gcgccccgcc gccttcacgc ccggtgatat
     8521 aggcgttggg ctcaccctgc ttgcgcagca ggtcctcgat cagtccggcc agaccggccg
     8581 cgcccttgcc gaacacgctt tcggccgcgc ccagcacgtc gtcgcgctga taggtgccgg
     8641 ggttctcggg aacggctccg gctggcgtct cggctgggcc ggtgtccggg gtggtcacgg
     8701 tctcgccggg cacggccgcc gaatcggccg gcggggtcgg cacaatggtc ggcgccgggc
     8761 tcggcgtgcc ttggggaggc gtcagatcgc cgtcgatcgc ccggtctgga tcgaccggct
     8821 ggacctgggc cggggccgag accgaagcgg ccagcgccac ggcgccgaac agcgaaaaca
     8881 gcgctccgcg aactctgcgc ggcatgccca cgatcatcgc acgatcctcc cgaatgcggc
     8941 cggattggcc aacaccccta gagaatcgat gccgccttgc cgggcaatga acgggcgacg
     9001 aggtgagtcg gttttgcggc caagcgagtc ccccgcgcgc cgcgccgggc cagcgcaatt
     9061 tctttgccgc gccccccttg ccgccccgca gagccaccgc tataggccgc gcttcgccgc
     9121 tgcgatccgg agacgtgggt gagtggctga aaccaacggt ttgctaaacc gtcgtactgg
     9181 taaatccggt accgagggtt cgaatccctc cgtctccgcc a
//
LOCUS       DASUDV010000470         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1783476,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000470 DASUDV010000000
VERSION     DASUDV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783476"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFV30_10190"
     CDS             <1..490
                     /locus_tag="VFV30_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4848502.1"
                     /translation="AVDPPATGRGDACGIVVCAIGADGLGRVLADCSLEKPSPERWAR
                     KVAQVAQQWQADRVVAEANQGGDMVGTVLRAADVALPLRLVHARKGKAARAEPVAALY
                     EAGRVRHAGLFAQLEDQLCGLMPGGEYQGPGRSPDRADALVWGLTELMLGYSRTPRIH
                     QT"
     gene            505..1665
                     /locus_tag="VFV30_10195"
     CDS             505..1665
                     /locus_tag="VFV30_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX4848503.1"
                     /translation="MSFLQTLASAFKGGGSSRVPLARSFTSPWFYADEASARAPYEYS
                     RSVRRAYLENPVAQRSVRLVAEGIGGAPLAPCDPELARLVSATSGGQALLETLASQLL
                     LHGNAYVQIMKDGRGRPAELYALRPERVSVVAGSDGWPAAYAYKVGETTLTIPALDED
                     ASPNLIHIRTFHPLDDHYGAGCLAAAEQAVASHSAAAAWNRTLLENAARPSGALMLDS
                     GDGGALSPDQFDRLKAELASAYSGAVNAGRPLLLEGGLKWQALSLSPADMDFAELKAA
                     AARDIALAFGVPPMLLGLPGDATYANYREANRALWRLTLLPLAGKILAALSEGLEPWF
                     AGERLAIDLDRVPALAEDRERLWAQVSAAAFLSDAEKRAALGLAQKDAENKQ"
     gene            1662..1970
                     /locus_tag="VFV30_10200"
     CDS             1662..1970
                     /locus_tag="VFV30_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6127 family protein"
                     /protein_id="HEX4848504.1"
                     /translation="MNREEMVARLIAQAADDGGDLVSLRAIVEEASELGAERVLARMG
                     LDDAEAHSDLSELRQLLQAWRDAKASAWKAAVDWVVRGLLALLLVGIAWRTGATGLLK
                     "
     gene            1973..2362
                     /locus_tag="VFV30_10205"
     CDS             1973..2362
                     /locus_tag="VFV30_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX4848505.1"
                     /translation="MRFAGYAALFDKRDAGRDTIRPGAFARTLAERTAPLPLFWQHRP
                     DLCIGWVESAAEDAKGLRIIARIDNPEGGAAAALRRGTVTGLSFGYRARRFTRDAQGR
                     ELTDLDLFEVSLVTHPMQHAARVHLLA"
     gene            2419..3555
                     /locus_tag="VFV30_10210"
     CDS             2419..3555
                     /locus_tag="VFV30_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX4848506.1"
                     /translation="MENEVPEEALDASFDLVARQEKTEAAVVSLRSDVEEVKSRLDKV
                     SRAAARPVIAIASQASPELKGFVDGYLRQGRTGELKSISGAVAADGGFAVPREIDQMI
                     ATQLRNISPIRAIAQVVQTGTSGYRKLVTSGGSASGWVSEVAARPETTTPKFNEIAPP
                     MGELYANPAASQAMIDDADFNLEEWLANEIAAEFARAEGAAFINGTGTNQPKGFLQAT
                     TALTGDATRAFGTLQHVVSGNASGFDTAPELKLIDLVHSLKSGHRQGAVWLMNSKTLS
                     TVRKFKAADGTFLWQPGIMDGAPARLLGYPVIEAEDMPDVAANALPIAFGNFKAGYLI
                     AERKTTTILRDPFTNKPFVNFYATKRVGGQVLDSDAIKLLKISV"
     gene            3656..4219
                     /locus_tag="VFV30_10215"
     CDS             3656..4219
                     /locus_tag="VFV30_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848507.1"
                     /translation="MKRAIVTPFTLAPGALGELKDWLGITTGSDDAQLGTLLRASIET
                     CEQFTGQMPLQQLCEEVLSASAAWQVLSVTPVQAITQVEGIPAEGSRFVLAAGAYEIE
                     LDADGPARVRVTSPGSAGRIAVRFTAGLAASWSALPETLRHGVIRLAAHHYRAREDSV
                     STGATPPAAPPAAIAALWRPWRRMRLA"
BASE COUNT          657 a         1475 c         1433 g          657 t
ORIGIN      
        1 ggcggtcgat ccgcccgcca cgggccgcgg cgatgcctgc gggatcgtgg tctgcgcgat
       61 tggagcggat ggccttggcc gggtgctggc cgattgcagc ctggaaaagc ccagccccga
      121 acgctgggcc cgcaaggtcg cccaggtcgc ccagcaatgg caggccgacc gggtggtggc
      181 cgaagccaac cagggcggcg acatggtcgg cacggtgttg cgcgccgccg atgtggcgct
      241 gccgctcagg ctggtccacg cccgcaaggg caaggccgcc cgcgccgaac cggtcgccgc
      301 gctttacgaa gctggccggg tgcgccacgc cgggctcttt gcccagctcg aagaccagct
      361 ctgcggcctg atgcccggcg gcgaatacca gggccccggc cgctcccccg accgcgccga
      421 cgcgctggtc tgggggctga cggaattgat gctgggatac agccgcacgc cccggatcca
      481 ccagacctga caaaaggaaa tcgcatgtcc ttcctgcaaa ccctggcctc cgccttcaag
      541 ggcgggggtt cttcgcgcgt gcctttggcc cgttcgttca cctcgccctg gttctatgcc
      601 gacgaggcat cggcgcgcgc accatatgaa tattcgcgtt cggtgcggcg cgcttacctt
      661 gaaaacccgg tggcgcagcg cagcgttcgg ctggtggccg aagggatcgg cggggcgccg
      721 ctggccccgt gcgatcccga gctggcgcgg ctggtttcgg ccaccagcgg cgggcaggcg
      781 ctgcttgaga ccttggccag ccagttgctg cttcacggca atgcctatgt ccagatcatg
      841 aaggatgggc gcgggcgccc ggccgagctt tatgccctgc ggcccgagcg ggtttcggtc
      901 gtggcaggca gcgatggctg gcccgctgcc tatgcctaca aggtgggcga aaccacgctg
      961 acgatccccg cgctggatga ggatgcatcg cccaatctga ttcatatccg cacctttcac
     1021 ccgctcgacg atcactatgg cgccgggtgc ctggcggcgg ccgaacaggc cgtggcgagc
     1081 cacagtgcgg ctgcggcatg gaaccgtacc ctgctggaaa acgcggcgcg gccatctggc
     1141 gcgctgatgc tcgattccgg cgatggcggt gcgctttcgc ccgaccagtt cgaccggctc
     1201 aaggccgaac tcgccagcgc ctattcgggc gcggtcaacg ccgggcggcc gctgctgctg
     1261 gagggcgggc tgaagtggca ggcgctcagc ctgtcgcccg ccgacatgga ctttgccgag
     1321 ctgaaagccg cggccgcgcg cgatatcgcg ctggcgttcg gcgtgccgcc gatgctgctg
     1381 ggcctgccgg gggacgccac ctatgccaat taccgcgagg cgaaccgcgc gctctggcgc
     1441 ctgacgctgc tgccgctggc gggcaagatc ctggccgcgc tgtccgaagg gcttgagccc
     1501 tggtttgccg gcgaacggct ggcgatcgac ctcgatcggg tgccggcgct tgccgaagac
     1561 cgcgagcggc tgtgggcgca ggtcagcgcc gccgccttcc tgtccgacgc tgaaaagcgg
     1621 gccgcgctcg gtctggcgca gaaggatgcg gagaacaaac aatgaacaga gaagagatgg
     1681 ttgcccgcct gatcgcccag gcggccgatg atggtggcga cctggtttcg ctgcgcgcga
     1741 tcgtcgagga agccagcgag ctcggcgccg agcgggtact cgcgcgcatg gggctcgatg
     1801 atgccgaggc ccattccgac ctgtccgagc tgcgccagct gctccaggcc tggcgcgatg
     1861 ccaaggccag cgcctggaag gcggcggtgg actgggtggt gcgcggcctg ctggcgctgc
     1921 tgctggtcgg gatagcctgg cgcaccggcg cgacggggct gctcaagtga gtctgcgctt
     1981 tgccggctat gccgcgctgt tcgacaagcg cgatgccggg cgcgacacga tccgccccgg
     2041 cgccttcgcc cggaccctgg ccgaacgcac cgcgccgctg ccgctgttct ggcagcaccg
     2101 cccggacctg tgcatcggct gggtcgaaag cgccgccgag gatgccaagg gcctgaggat
     2161 catcgcccgg atcgacaatc ccgaaggcgg tgccgccgcc gcgctgcgcc gcggcacggt
     2221 gaccgggctg tcgttcggct accgcgcccg ccgtttcacc cgcgatgccc agggccgcga
     2281 actgaccgat ctggatctgt tcgaggtcag cctggtcacc cacccgatgc agcacgccgc
     2341 gcgggttcat ctcctcgcct agtccctccc ccctcaccct cccgtggcgg ggagagttca
     2401 acagaaaggt aatgccccat ggaaaatgaa gtgcccgaag aagcgcttga cgcttcgttc
     2461 gatctggtcg cgcgccagga aaagaccgaa gccgcggtgg tttcgctgcg cagcgatgtc
     2521 gaggaagtga aatcgcgcct cgacaaggtc agccgcgccg cggcccgccc ggtgatcgcg
     2581 atcgcctcgc aggccagccc cgagctcaag ggctttgtcg acggctatct gcgccagggc
     2641 cgcaccggcg aactgaagtc gatcagcggc gcggttgcgg ccgatggcgg ctttgccgtc
     2701 ccgcgtgaga tcgaccagat gatcgccacc cagctcagga acatcagccc gatccgcgcc
     2761 atcgcccagg tggtccagac cggcacctcg ggctatcgca agctcgtcac ctcgggcggt
     2821 tcggcctcgg gctgggtcag cgaagtggcg gcgcggcccg aaaccaccac gcccaagttc
     2881 aacgaaatcg ccccgccgat gggcgaactc tatgccaatc cggcagccag ccaggcgatg
     2941 atcgacgatg ccgatttcaa cctggaagaa tggctggcga acgaaatcgc cgccgaattc
     3001 gcccgggccg aaggcgccgc cttcatcaac ggtaccggca ccaaccagcc caagggcttc
     3061 ctccaggcca ccaccgcgct gaccggcgat gccacccgcg ccttcggcac gctgcagcac
     3121 gtggtttcgg gtaatgcctc gggcttcgat accgcaccgg aattgaagct gatcgacctg
     3181 gtccattcgc tcaagtcggg tcaccgccag ggcgcggtgt ggctgatgaa ctcgaagacg
     3241 ctgtcgaccg tgcgcaagtt caaggccgcc gacggcacct tcctgtggca gcccgggatc
     3301 atggacggcg ccccggcccg cctgctcggc tatccggtga tcgaggccga agacatgccc
     3361 gacgttgccg ccaacgcgct gccaatcgcc ttcggcaact tcaaggccgg ctacctgatc
     3421 gcggaacgca agacgaccac gatcctgcgc gatccgttca ccaacaagcc cttcgtcaac
     3481 ttctacgcga ccaagcgggt gggcggacag gtgctcgaca gcgacgcgat caagctcctg
     3541 aagatctcgg tctgatccac aaccgggcgg gcctcccctc ccgccccgcg tgcccgcgcc
     3601 gtctccctcg cggcgcgggc acgccccctt tttccaggac aacatggaga agcccatgaa
     3661 acgggcaatc gtcacgccct tcacgctggc gccgggcgcg cttggcgagc tcaaggactg
     3721 gcttggcatc accaccggca gcgacgatgc ccagcttggc accttgctgc gcgccagtat
     3781 cgaaacctgc gagcagttca ccggacagat gccgctgcag cagttgtgcg aggaggtgct
     3841 gagcgcctct gccgcttggc aggtgctgtc cgtcacgccg gtccaggcga tcacccaggt
     3901 cgaaggcatt ccggccgagg ggtcgcgctt tgtcctggcg gccggcgcct acgagatcga
     3961 gcttgatgcc gatggtccgg cgcgggtccg ggtgaccagt cccggcagcg cggggcgcat
     4021 cgcggtgcgc ttcacggccg ggctggcggc cagctggtcg gccttgcccg aaacgctgcg
     4081 ccatggcgtg atccgccttg ccgcccacca ctatcgtgcc cgcgaggata gcgtgtcgac
     4141 cggcgccacg ccgcctgccg cgccgcctgc cgcgatcgcc gcgctgtggc gcccctggcg
     4201 ccggatgcgc ctggcatgag ca
//
LOCUS       DASUDV010000471         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1672917,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000471 DASUDV010000000
VERSION     DASUDV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672917"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFV30_10220"
     CDS             <1..449
                     /locus_tag="VFV30_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX4848508.1"
                     /translation="AHSVAAKAAIVKADERETLGLRALLNLGHTFGHALEAQTGFSDR
                     LLHGEGVALGMVLAARFSARRGLIAGSDAERIAAHFANVGMASEIATLGLNCDGQALV
                     AHMLHDKKMDAGTLPFVLLRQIGEAFLAKDVDLAEVAAFLDGELSR"
     gene            601..2265
                     /locus_tag="VFV30_10225"
     CDS             601..2265
                     /locus_tag="VFV30_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4848509.1"
                     /translation="MSSARRQVTVFEAARFITMDPALPEARFVAVADGMILGHAQNLD
                     ALAPWTRGQDVTLNRDFAGKVLMPGLIDPHVHPMLAAVMLNLPFIAPDDWTLPSGRYP
                     GAQTPQAWHDRLKAELARSQADPFICWGYHQLFHGPLDRRALDAIAPDRAVVIWQRSF
                     HDVIANSAALKAWGLADAASFAAALAAAKADPRDSDFDKGLFSETGLPVALAKMQPAL
                     LGGTRVRDGMAALQRMMLTSGVTTVSDMGTGIFADFATEAGLIRAAFERPGNPSRIML
                     MPLAHQVPADADLSAYLTGIESRFAGHHVRVDRRVKLLADGAFFALNMRMNAPGYTDG
                     HSGKWITPPDVLDALAQRFWRAGFSLHIHVNGDEGLDVTLATTARLEKRAGQTLTFEH
                     LGYSTEEQNRRIAQQGLMVSAQPNYIRVLGDAYALEGLGPDRAATINRLGSLERKAIP
                     LGLHSDFNMAPIDPLYLAWIAANRLTLAGHRQAPYERLSLDKALRAITIEAAQVIGMD
                     HLVGSIRAGKRADFTILDRDPSKAGAARLGEVKVEGVIFEGSVTRV"
BASE COUNT          385 a          785 c          750 g          401 t
ORIGIN      
        1 tcgcccattc tgtcgcggcc aaggcggcga tcgtcaaggc tgacgagcgc gagacgctgg
       61 gcctgcgcgc cttgctcaac ctgggtcaca ctttcggcca tgcgttggaa gcgcagaccg
      121 gcttttccga ccgcctgctc cacggtgaag gcgtggcgct tggcatggtc ctcgccgcac
      181 ggttctcggc ccggcgcggg ctgatcgcgg gtagcgatgc agaacggatc gccgcccatt
      241 tcgccaatgt cggcatggcg agcgagatcg ccaccctcgg cctcaattgt gacggccagg
      301 cccttgtcgc gcacatgctg catgacaaga agatggacgc cggcaccctg cccttcgtcc
      361 tcctgcgcca gattggcgag gccttcctgg cgaaggacgt ggacctggca gaggtcgccg
      421 cattccttga tggtgaattg agccgctgat ccggcacggc cgcgcttgcc accgccccca
      481 agcgcgtggc agcctttgcc ccggataggg tgcaaggggg aaagcggcat ggcgggattc
      541 gaccggcgat cgacaatatt gggccttgcc gcgctgcccc tgatcggcgc cgccccgaca
      601 gtgtcaagcg ccagacgcca agtcaccgtg ttcgaggcag cccggttcat cacgatggac
      661 ccggccctgc ccgaagcgcg ctttgtggcc gtggctgacg ggatgatcct gggccacgcc
      721 cagaacctgg acgccctcgc cccctggacg cgcggccagg acgtaacgct caaccgcgat
      781 ttcgcgggca aggtcctgat gccgggcctg attgatccgc atgtccaccc gatgctggcg
      841 gcggtcatgc tcaacctgcc attcatcgca ccggatgact ggaccctgcc atcgggccgc
      901 tatccaggcg cgcaaacgcc gcaggcctgg catgatcggc tgaaggcaga actcgcccgg
      961 tcgcaggccg atccgtttat ctgctggggc tatcaccagc tgttccacgg cccgctcgat
     1021 cgccgcgcgc tggatgccat tgcgccggac cgcgccgtgg tgatctggca gcgcagcttt
     1081 catgatgtga tcgccaattc cgccgcgctg aaggcctggg gactggcaga tgcggccagc
     1141 tttgccgctg cccttgccgc cgccaaggcc gatccgcgcg acagcgattt cgacaagggc
     1201 ctattttccg agaccggcct gcccgtggcg ctggccaaga tgcaaccggc cctgctgggc
     1261 ggcaccaggg tgcgcgatgg catggccgcg ctgcaacgga tgatgctgac cagcggggtg
     1321 accaccgtct ccgacatggg caccgggata ttcgccgatt tcgccaccga agccggcctg
     1381 atccgcgccg ccttcgaacg gccgggcaat ccatcgcgga tcatgctgat gccgctggcc
     1441 catcaggttc cggccgatgc cgatctgtca gcctacctga ccgggatcga aagccgcttc
     1501 gccggacacc atgtccgggt cgatcgccgg gtcaagctgc tggcggacgg tgcgttcttt
     1561 gcgctcaaca tgcggatgaa cgctcccggc tataccgatg gccatagcgg caaatggatc
     1621 acgccgccgg acgtgcttga tgcgcttgcc caacggttct ggcgggccgg tttctcgcta
     1681 cacatccacg tcaacggcga tgagggcctg gacgtgaccc tggcaacgac ggcgcggctc
     1741 gagaagcggg cgggacagac gctgacgttc gaacacctgg gctattcgac cgaggagcag
     1801 aaccgccgca ttgcccagca ggggctgatg gtctcagccc agccgaacta tatccgcgta
     1861 ctgggcgatg cctatgcgct tgaagggctt ggtccggacc gcgcggccac gatcaaccgg
     1921 ctcggctcgc ttgagcgcaa ggctatcccg ctggggctgc acagcgattt caacatggcg
     1981 ccaatcgatc cgctgtacct cgcctggatc gcagccaacc ggctgacgct cgccggccat
     2041 cgccaggcac cctacgaacg gctctcgctc gacaaggcct tgcgcgcgat cacgatcgaa
     2101 gctgcccagg tgatcgggat ggatcacctg gtcggatcga tccgggccgg caaacgcgcc
     2161 gatttcacca tcctcgatcg cgatccttct aaggccgggg ccgcccggct aggcgaagtg
     2221 aaggttgaag gcgtgatttt cgaaggttcg gtgaccaggg tctagggcct tcgccccacg
     2281 gccgcatatt tggcgctagc gcggcggcag cactgccggc a
//
LOCUS       DASUDV010000472         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1341322,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000472 DASUDV010000000
VERSION     DASUDV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341322"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..558)
                     /locus_tag="VFV30_10230"
     CDS             complement(19..558)
                     /locus_tag="VFV30_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HEX4848510.1"
                     /translation="MAVDPARPFKPVKIAVLTVSDTRDMNSDTSGALLVERVQSAGHQ
                     LTTRAIEKDDVGAICSRINTWIDEQLADAIVITGGTGLTGRDVTPEALDRLMAEHGGK
                     QIPGFGELFRWISYASIGTSTIQSRACAVLVRGVYIFALPGSNGAVKDGWDKILAEQF
                     DSRYGPCNFVELMPRLREV"
     gene            complement(548..2299)
                     /locus_tag="VFV30_10235"
     CDS             complement(548..2299)
                     /locus_tag="VFV30_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4848511.1"
                     /translation="MLAGASAGLAPQLAHANSAAVDYFRNRAARSVVPSVLTQDERNF
                     YRDLFGAIDKEDWAKVQTLFAQRADGPLHQVARAEYFLAANSPRIEGDALAAWLARGT
                     DLPQAEQISNLAAKRGVSALPMLPAPNALASLPSRSKRIRPRETADGTMPGTVAAAIN
                     GKIKADDPAGAKAVLDGIDTQLSPYARAEWRAKVAWSFYIENDDASAYALAQTAQDGA
                     GPWVAEGWWTAGLAAFRLDDCAGASDAFARAAGFSENAELTSAALYWQSRALVRCRQP
                     DKASAPLKQAARMDETLYGMLAAEQLGMALPKDHEAPDFSQNDWQALREVPNVRTAVA
                     LAEIGESGLADEVLRHQARIGAVQQHQPLSRLARDLGVPGTQLWMAYNAPYGAKSEPA
                     LRFPTPKWTPANGWKVDPALVYAHALQESVFRASAVSPAGARGLMQIMPAAARDHAAS
                     IGVAGTAHDLGRPEINLAFGQNHLQMLGNSAATQGLLPKVMAAYNAGLTPIGRWQYEI
                     RDKGDPLLWIESVPYWETRGYVNIVMRNYWMYERQAGGPSESRLALVQGMWPTFPGLG
                     AAQAVRMNGNGVIVRGR"
BASE COUNT          378 a          783 c          794 g          405 t
ORIGIN      
        1 cgtattgccg cgaaggcttc agacctcgcg cagtctgggc atcagttcga cgaagttgca
       61 gggcccgtag cggctgtcga actgttcggc gaggatcttg tcccagccgt ccttcaccgc
      121 gccgttcgat ccgggcagcg cgaagatata gaccccgcgc accagcaccg cgcaggcccg
      181 cgactggatc gtcgaggtgc cgatcgaggc atacgaaatc cagcggaaca gctcgccaaa
      241 gccggggatc tgcttgccgc cgtgttcggc catcagccga tcgagcgctt cgggcgtgac
      301 atcgcggccg gtcagcccgg tgccgccggt aatcacgatc gcatcggcga gttgttcatc
      361 gatccaggtg ttgatccggc tgcagatcgc gccgacatcg tccttctcga ttgcccgggt
      421 ggtcagctgg tggccggccg actggacccg ttcgaccagc agcgcgcccg aggtgtcgga
      481 gttcatgtcg cgcgtgtcgg aaacggtcag caccgcgatc ttcaccggct tgaacgggcg
      541 ggcgggatca acggccacga acgatcactc cattgccatt cattcgcacc gcctgggctg
      601 cgcccagccc cgggaaggtc ggccacatac cctggacaag cgccaggcgg ctttccgaag
      661 gaccgcctgc ctggcgttcg tacatccagt agttgcgcat cacgatgttc acatagccgc
      721 gcgtttccca atagggcacg ctttcgatcc acagcagcgg atcgcccttg tcgcggatct
      781 cgtactgcca gcgcccgatc ggagtaaggc cggcgttgta ggcggccatg accttgggca
      841 gcaggccctg ggtcgcggcg gaattgccca gcatctgcag gtggttctgg ccgaacgcca
      901 gattgatttc gggccgaccc agatcgtgcg cggtgccggc aacgccaatc gatgcggcgt
      961 gatcccgggc cgcggcgggc atgatctgca tcagcccgcg cgcgccggcc gggctgaccg
     1021 cgctggcacg aaacaccgat tcctgcaggg catgggcata gaccagcgcc gggtcgacct
     1081 tccagccatt ggccggggtc cacttggggg tgggaaaccg cagcgccggc tcgctcttcg
     1141 ccccataggg cgcgttgtag gccatccaca gctgggtgcc aggaacgcca agatcgcggg
     1201 ccaggcgcga aagcggctgg tgctgctgca ccgcgccgat ccgggcctgg tggcgcagca
     1261 cttcgtcggc caggccgctt tcgccaattt cggccagggc cacggcggtg cgcacgttgg
     1321 gcacttcgcg cagcgcctgc caatcgttct ggctgaaatc gggcgcttcg tggtccttgg
     1381 gcagcgccat gccgagctgt tcggcggcga gcatgccata aagcgtttcg tccatccggg
     1441 ctgcctgctt cagcggtgcg ctggccttgt ccggctggcg gcagcgaacc agcgcgcgcg
     1501 attgccagta gagcgcggcc gaggtcagtt cggcgttctc cgaaaatccc gcagcccggg
     1561 caaaggcatc gctggcgccg gcacaatcat cgagccggaa cgcggcaagc cccgcagtcc
     1621 accagccttc cgcgacccag gggcccgcgc cgtcctgcgc ggtttgcgcc agggcatagg
     1681 ccgaggcatc gtcgttctcg atatagaagc tccaggccac cttggcgcgc cattcggccc
     1741 gggcataggg tgaaagctgg gtgtcgatcc cgtcgagcac cgccttggcg ccggccggat
     1801 cgtcggcctt gatcttgccg ttgatcgccg cggcgaccgt gccgggcatg gtgccatcgg
     1861 cggtttcgcg cgggcggatc cgcttcgacc ggctgggcag gctggccaag gcattgggcg
     1921 cgggcagcat cggcaaggcg gaaaccccgc gcttggcggc cagattgctg atctgttcgg
     1981 cctggggcag atcggtaccg cgggccagcc atgcggcaag ggcatcgccc tcgatccggg
     2041 gggagttcgc agccagaaag tattcggctc gcgccacctg gtgcagcggg ccatccgcgc
     2101 gctgggcaaa cagggtctga accttggccc agtcttcctt gtcgattgcg ccgaacagat
     2161 cgcggtagaa attgcgctcg tcctgggtga gcaccgatgg cacgaccgaa cgcgccgcgc
     2221 ggttgcggaa atagtcgacc gccgcgctgt tcgcatgggc cagctgcggg gcaagcccgg
     2281 cgctggctcc ggcaagcaga gcggcgatcc gaaatgcaac cttgaaacgc actacagcct
     2341 tgttcctcaa tcgttttcct
//
LOCUS       DASUDV010000473         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_657026,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000473 DASUDV010000000
VERSION     DASUDV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657026"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFV30_10240"
     CDS             complement(<1..281)
                     /locus_tag="VFV30_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEX4848512.1"
                     /translation="MFRPLTDSLSVSPQITVADVAEAARLGFRMIVNNRPEDESDDQT
                     PGAAIEAAAREAGLDYVAIPITHAGFSEGQVSAMNAALDGAQGPVLAYCR"
     gene            597..1352
                     /locus_tag="VFV30_10245"
     CDS             597..1352
                     /locus_tag="VFV30_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX4848513.1"
                     /translation="MGFDRGRRGRGRDKRDGFGEESFDPFAGGGDRFGGGDRGGYGGS
                     RGGFGGGDRGGYGGGGGDRGGFGGGPRGGGGGGFGGGAPRGGGMPAQVVGEGSGTVKF
                     FNSAKGFGFIQQDGGGEDVFVHISAVERAGLEGLAEGQQLKFQLVDRGGKVSASDLEV
                     VGDVIPVAKREAAPQRQLTGEKATGTVKFFNAMKGFGFITRDDGQPDAFVHISAVERS
                     GMSGLNEGDRLEFDIEVDRRGKYSAVNLMPRQD"
     gene            1487..2680
                     /locus_tag="VFV30_10250"
     CDS             1487..2680
                     /locus_tag="VFV30_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4848514.1"
                     /translation="MSITPLMPVYPRCGFRPVRGDHCHLIGEDGQRYLDFAAGIAVNL
                     LGHSHAGLIGAIQKQAETLMHVSNLYGSPQGEHLAQRLVDLTFADTVFFTNSGAEAVE
                     CAIKTVRAYHQHAGNDHKFEIITFKNAFHGRTMATISASNQEKMHKGFLPLLQGFKYV
                     DFDDLEGAKAAIGPNTAGFLVEPVQGEGGIRPASAAFMQGLRALADEHDLLLALDEVQ
                     CGVARTGTLYAYEQFGIEPDVVATAKGLGGGFPIGACLATEKAARGMVIGTHGSTYGG
                     NPLAMAAGEAVLYAVANPEFLGEVQAKAERLRGRLEQFIGNYPELFESVRGMGLMLGV
                     KLKMEPRALVTHLRDNHQLLTVAAGDNTLRLVPPLVIDDSHIDEFFDKLSAGAASFQP
                     PEAAS"
     gene            2677..3603
                     /gene="argF"
                     /locus_tag="VFV30_10255"
     CDS             2677..3603
                     /gene="argF"
                     /locus_tag="VFV30_10255"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX4848515.1"
                     /translation="MTRHFLNLSDAGSDALAAMLNDALERKAYRRDVPKGAPDGDAPL
                     AGRLLAMVFEKNSTRTRVSFDVAMRQLGGSAIVLDAGTTQLGRGETIADTARVLSRMV
                     DAIMLRTDDHAKIEELAHYADVPVINGLTDSSHPCQIMADLLTLLERGKALPGLELAW
                     LGDGNNVLNSLIEAAGLFGFNIRAGIPQGYEPDAGFIARAEARGARITLTRSAAEAAA
                     GADVVVTDTWVSMGQDHAHNKLAAMAPYQVNEALMAAAKSDAAFLHCLPAHRGEEVTD
                     AVIDGPRSAVWDEAENRIHAQKSVLRWVFGQL"
     gene            3600..>4189
                     /locus_tag="VFV30_10260"
     CDS             3600..>4189
                     /locus_tag="VFV30_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX4848516.1"
                     /translation="MSAPEHIAAGETGYDRVLAFTIPERNARGRAVRLGPVLETILAA
                     HAYPTPIKHLLAEALVLTALVGSLLKDGEGQLTMQAQTQGGVVDLLVCDYRGGEVRGY
                     VRHDAERLAMLGANPSLFALFGQGGYLAITFDLAATGQRYQGIVPLEGSSLAEACEAY
                     FAQSEQVPTLIRVGVRSDAAGCVAGGLLAQHLPDGE"
BASE COUNT          667 a         1350 c         1494 g          678 t
ORIGIN      
        1 cggcaatagg ccaggaccgg cccctgcgcc ccgtccagcg cggcgttcat cgcgctgacc
       61 tggccttcgg aaaagccggc gtgggtgatg ggaatggcca catagtccag cccggcctcg
      121 cgcgctgccg cttcgatcgc ggcgccgggg gtctgatcgt cgctttcgtc ctcgggccgg
      181 ttgttgacga tcatccggaa gcccagccgg gccgcctcgg ccacgtcggc aacggtgatc
      241 tggggactga cggacaggct atcggtcaag ggacggaaca tcggcaacgg gcctccttgc
      301 cgggcgctgt gccaagccgc gggcggactg gcaagcttgg tgcgcgccgg cgcagcattc
      361 cgttgccgcc gggcgggatg gcgacgcaat gttgctggca gaaatgtcac acttgtgcgg
      421 cggccgtgcc ggtggcggcg aggctcggcg aatccgttcg ccatagccct gcaaatggtc
      481 tatgtggaaa agtgcagcgg agggggccct acggcgcttg accctgtcga ccggcgcgat
      541 tcgtccgcgc cgccgggcgt gtagcattgg ggcgaagcga aggtacgact aggtttatgg
      601 gtttcgatag agggcgtcgc ggccgtggac gcgacaagcg ggatgggttc ggagaagaaa
      661 gtttcgatcc ctttgctggc ggtggtgacc ggttcggcgg cggtgatcgc ggtggttacg
      721 gcggatcgcg cggcggcttc ggcggcggcg accgcggtgg ttacggtggc ggcggcggtg
      781 atcgcggcgg cttcggtggc ggcccgcgcg gcggcggtgg cggcggcttc ggcggcggtg
      841 caccgcgcgg cggcggcatg ccggcccagg tggtcggcga aggctccggc acggtcaagt
      901 tcttcaattc ggccaagggc ttcggcttca tccagcagga tggcggcggt gaggacgtgt
      961 tcgtccacat cagcgcggtc gagcgcgccg gcctcgaagg cctggccgaa gggcagcagc
     1021 tcaagttcca gctggtcgat cgcggcggca aggtctctgc cagcgacctc gaagtcgtcg
     1081 gcgatgtcat cccggtggcc aagcgcgaag cggctccgca gcgccagctg accggcgaga
     1141 aggcgaccgg tacggtcaag ttcttcaacg ccatgaaggg ctttggcttc atcacccgtg
     1201 atgacggcca gccggatgcg ttcgtgcaca tcagcgcggt cgaacgctcg ggcatgtcgg
     1261 gcctcaacga aggtgaccga ctcgagtttg atatcgaggt cgatcgacga ggcaagtact
     1321 cggcggtcaa cctgatgccg cgtcaggatt gatcccgacc gcatcgcttc cgggggttgt
     1381 cccggagcct gccaagttct aaacgcgtgc agatggcggc ccttccgggg ccgccatttg
     1441 tcgttttcgt tttcccgcta aatccagtga agcgaaggaa gtacccatgt cgattactcc
     1501 gctgatgcca gtctatcccc gttgcggctt ccggccggtg cgcggtgacc actgccacct
     1561 gatcggggaa gacggacagc gatacctgga tttcgcggcc ggcatcgccg tcaacctgct
     1621 gggccattcg cacgccggcc tgatcggcgc gatccagaag caggccgaga cgctgatgca
     1681 cgtctccaac ctctatggca gcccgcaggg cgaacacctg gcgcagcggc tagtcgacct
     1741 gacctttgcc gacacggtgt tcttcaccaa ttcgggcgcg gaagcggtgg aatgcgcgat
     1801 caagaccgtg cgggcctatc accagcacgc cggcaacgat cacaagttcg agatcatcac
     1861 cttcaagaac gccttccacg ggcggaccat ggcgacgatc agcgcctcca accaggagaa
     1921 gatgcacaag ggcttcctgc cgctgctgca gggcttcaag tatgtcgact tcgacgacct
     1981 ggaaggcgcc aaggcggcga tcggccccaa caccgccggg ttcctggtgg agccggtgca
     2041 gggcgaaggc gggatccgtc cggcctcggc cgcattcatg cagggcctgc gcgccctggc
     2101 tgatgagcac gatctgctgc tggcgctgga cgaagtgcag tgcggcgtgg cccgcaccgg
     2161 cacgctctat gcctatgagc agttcgggat cgagccggac gtggtcgcca cggccaaggg
     2221 cctgggtggc ggtttcccga tcggggcctg cctcgccacc gaaaaggccg cgcgcggcat
     2281 ggtgatcggc acccacggat cgacctatgg cggcaacccg ctggccatgg cggccggcga
     2341 ggcggtgctt tatgccgttg ccaaccccga gttcctgggc gaggtgcagg ccaaggccga
     2401 gcgtctgcgc ggccggctgg agcagttcat cggcaactat ccggaactgt tcgaaagcgt
     2461 ccgcggcatg ggcctgatgc tgggcgtgaa gctgaagatg gaaccgcgcg cgctggtgac
     2521 gcacctgcgc gacaaccacc agctgctgac cgtggcggcc ggcgacaaca ccctgcggct
     2581 ggttccgccg ctggtcatcg atgacagcca catcgacgaa ttcttcgaca agctgtccgc
     2641 cggtgcggcc agcttccagc cgcccgaagc cgcgtcatga cccggcattt cctgaacctt
     2701 agcgatgccg gcagcgatgc gctggcggcg atgctgaacg acgcgctgga gcggaaggcc
     2761 tatcgccgcg acgtgcccaa gggcgcgccc gatggcgatg cgccgctggc cgggcgcttg
     2821 ctggccatgg tgttcgagaa gaactccacc cgcacccgcg tctccttcga tgtcgcgatg
     2881 cgccagctgg gcggctcggc catcgtgctg gatgccggca cgacccagct gggccgcggc
     2941 gaaaccattg ccgataccgc gcgggtgctg agccggatgg tcgatgcgat catgctgcgg
     3001 accgacgatc acgccaagat cgaggagctg gcgcactacg ccgacgtgcc ggtgatcaac
     3061 gggctgaccg acagttcgca cccgtgccag atcatggccg atctcctcac cctgctggaa
     3121 cgcggcaagg ccctgcccgg gctggagctg gcctggctgg gggacggcaa caacgtcctc
     3181 aactcgctga tcgaggcggc gggcctgttc gggttcaaca tccgtgccgg gatcccacag
     3241 ggttatgagc cggacgccgg cttcatcgcc cgcgccgagg cccggggtgc ccgcatcacg
     3301 ctgacccgga gcgccgccga ggcggctgcc ggtgcggacg tggtcgtcac cgacacctgg
     3361 gtgtcgatgg ggcaggacca tgcccacaac aagctggcgg cgatggcccc ttaccaggtc
     3421 aacgaagcgc tgatggccgc ggccaaaagc gatgcggcgt tcctgcactg cctgcccgcg
     3481 caccgcggcg aggaagtgac cgacgccgtg atcgatgggc cgcgttcggc ggtgtgggac
     3541 gaggccgaga accggatcca cgcccagaag agcgtgctgc gctgggtctt cggccagctg
     3601 tgagcgcgcc ggagcacatc gcggcgggcg aaaccggcta tgaccgggtc ctggccttca
     3661 ccatcccgga gcgcaacgcg cgcggccggg cggtgcggct tggtccggtc ctggaaacga
     3721 tcctggcggc ccacgcctat cccacgccga tcaagcacct gctggccgag gccctggtgc
     3781 tgaccgcgct ggtcgggtcg ctgctcaagg acggggaagg ccagctgacc atgcaggccc
     3841 agacccaggg cggcgtggtc gatctgctgg tgtgcgatta ccgcggcggg gaagtgcgcg
     3901 gctatgtccg ccacgatgcg gagcggctgg ccatgctcgg cgccaatccc tcgctgttcg
     3961 cgctgttcgg gcagggcggc tatctggcga tcacgttcga cctggccgcc accggtcaac
     4021 gctatcaggg aatcgttccg ctcgagggca gctcgctggc cgaggcctgc gaggcctatt
     4081 tcgcccagtc cgagcaagtg ccgaccctga tccgggtggg cgtgcgatcg gacgcggcgg
     4141 gctgcgtggc gggggggctg ctggcccagc acctgcccga tggcgagga
//
LOCUS       DASUDV010000474         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1385607,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000474 DASUDV010000000
VERSION     DASUDV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385607"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..580)
                     /locus_tag="VFV30_10265"
     CDS             complement(2..580)
                     /locus_tag="VFV30_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4848517.1"
                     /translation="MHRPDTTIMAIHGKAPRIHDSAFIAPGCRIIGNVEIGPDASVWY
                     NCVLRGDANRIVVGARTNIQDGSVIHCDSPKPKRPEGIPTIIGEDVLIGHMAMVHGTV
                     LEDRAFVGIGAITMDGCHMESDSMLAAGAMLTPGKRIPSRQLWGGRPAAFMRELADPH
                     LADMQAGVQGYVLNARAHRAALAEAGDGAAED"
     gene            complement(583..>2239)
                     /locus_tag="VFV30_10270"
     CDS             complement(583..>2239)
                     /locus_tag="VFV30_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848518.1"
                     /translation="IALCARARLGLPNAAIPASARPADPIPPRRLAQWIVLAAVPSGL
                     MLSTTTHLTTDIFAMPLLWVIPLGIYLLSFVIAFADRRGPARVISALAWLAILSTGAL
                     AMISQGSNGLLPVIGGVVLLFFLCVALHARLYDLRPAPEQLTGFYLAMSAGGALGGLF
                     TALVAPLVFDWVWEHPLLVLAAALLLPRYGLLDWTRAKGLQPELKYMALGALVLTLAG
                     LLWLLLDARFQAEEDTTLIWALSAAIAVIGMGLVPWRGLAVLLLGATMLVQGGWLTVS
                     DSREGLRTRSYFGIYTVRDYPERKLRTLAHGTTLHGQQSTEPGMRCMPLSYYGPGSGV
                     AIGFANASRLFPSAPKLGVVGLGTGSLAGFARPGQSWTLFEIDQAVLGLSEQGKFTFL
                     RDCAPQAKVVLGDARLKLAEARAGAFDLLAIDAFSSDSIPLHLFTHEAFGVYLQALSP
                     RGILFVHISNRYMELEPALMAEVRARGLHAAVREDDPPQGGPWTGSTWVAVTRDPERL
                     RDLSALAPAMAWRPGKPPAPRVWTDDHASILPYVTWSNFLVRH"
BASE COUNT          391 a          764 c          784 g          300 t
ORIGIN      
        1 ctcagtcttc ggccgcgcca tcgcctgctt cggccagtgc cgccctgtgt gcgcgggcgt
       61 tgaggacgta gccctgcacg cccgcctgca tatcggccag gtgcgggtcc gcgagttcgc
      121 gcatgaaggc ggcgggccgt ccaccccaga gctggcgcga gggaattcgc ttgccgggcg
      181 tcagcatggc tccggccgcg agcatcgaat cgctctccat gtggcatcca tccatcgtga
      241 tcgcgccgat acccacgaag gcacggtctt cgagcacggt tccgtggacc atggccatgt
      301 ggccgatcag cacgtcctcg ccgatgatgg tcggaatccc ctcggggcgt ttcggcttgg
      361 gactgtcgca gtggatcacg ctgccgtcct ggatgttcgt gcgagcaccc accacgatgc
      421 ggttggcatc cccgcgcaag acgcaattgt accagaccga cgcatcgggc ccgatctcga
      481 cattgccgat gatccggcag ccgggcgcga tgaaggcgct gtcgtggatg cgcggggcct
      541 tgccgtggat cgccatgatg gtggtgtcgg gtcggtgcat gatcagtgcc tcacaaggaa
      601 gttggaccag gtgacatagg gcaggatcga ggcgtggtca tcggtccaga cccggggagc
      661 gggcggcttg ccggggcgcc aggccatggc cggggccagc gcggagagat cgcgcagccg
      721 ctccggatcg cgggtcacgg caacccaggt cgatccggtc cacggcccgc cctgcggcgg
      781 atcgtcctcg cgcaccgcgg cgtgcagacc gcgggcgcgg acttcggcca tcagcgccgg
      841 ctccagttcc atgtagcggt tggaaatgtg cacgaacagg atcccgcgcg ggctgagcgc
      901 ctgcagatag accccgaaag cctcgtgggt gaagaggtgc aagggaatcg aatcggacga
      961 gaaggcatcg atcgcgagaa gatcgaaggc cccggcccgg gcctcggcca gcttgagccg
     1021 ggcatcgccg agcacgacct tcgcctgggg ggcgcagtcc ctcaggaagg tgaacttgcc
     1081 ctgctccgac aggcccagca cggcctggtc gatctcgaac agggtccagc tctggcccgg
     1141 acgggcgaag ccggcgagcg atccggtgcc cagcccgacc acccccagct tgggcgcgga
     1201 ggggaacagg cgcgaggcgt tggcaaagcc gatcgccacg cccgaacccg ggccgtaata
     1261 gctgagcggc atgcaccgca tccccggctc ggttgattgc tggccgtgga gcgtggtgcc
     1321 gtgggccagg gtccgcagct tgcgttcggg gtaatcgcgg acggtgtaga tcccgaaata
     1381 gctgcgggtg cgcagccctt cgcggctgtc cgacacggtc agccagccgc cctggaccag
     1441 catggtcgcg cccagcagca gcacggccag cccgcgccac ggcaccagcc ccatcccgat
     1501 caccgcgata gctgccgaca gcgcccagat cagggtcgtg tcctcctcgg cctggaaccg
     1561 cgcatcgagc agcaaccaca agaggcccgc aagggtcagc accagcgcgc ccagcgccat
     1621 gtacttgagc tccggctgca ggcccttggc ccgggtccag tcgagcaggc cgtagcgcgg
     1681 cagcagcagc gccgcggcca gcaccagcag cggatgctcc cagacccagt caaacaccag
     1741 cggcgcgacc agcgcggtga acagcccgcc cagcgccccg cccgccgaca tggcgaggta
     1801 gaacccggtc agctgctcgg gcgcggggcg caggtcatag agccgggcgt ggagcgccac
     1861 gcagaggaag aacagcagca cgacgccgcc gatcaccggc agcagcccgt tggacccttg
     1921 cgagatcatc gccagcgccc cggtcgagag gatcgccagc caggccagcg ccgaaatgac
     1981 ccgcgccggg cctcgccggt cggcaaaggc gatcacgaaa ctgaggagat agatccccag
     2041 cgggatcacc cacaacagcg gcatcgcgaa gatatcggtg gtgaggtggg tggtggttga
     2101 cagcatcagc cccgaaggca ccgccgccag cacgatccac tgcgccagcc ggcgcggcgg
     2161 gatcgggtct gccggccggg ccgaggccgg aatggcggca ttgggcaggc caagccgggc
     2221 ccttgcgcaa agcgcgatc
//
LOCUS       DASUDV010000475         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_281256,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000475 DASUDV010000000
VERSION     DASUDV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281256"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFV30_10275"
     CDS             <1..606
                     /locus_tag="VFV30_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4848519.1"
                     /translation="VAARQNSLLIDLADIALMLPRVPEACAVNVAPTTSTTMQLALGD
                     ALAMAVMDMRGVSKNHLRALHPAGAIGLALAPVSEIMHGADRLPLVSRDAGMPEAISV
                     MTSGCFGLAGVTDADGTLVGIITDGDLRRKLGVLSTAFAGEVMTPAPKVIPAEMPAGE
                     ALVFLNDNKITAAFVVEDPAAGPQAPLGIIHIHDLLRHGLN"
     gene            618..1418
                     /gene="kdsB"
                     /locus_tag="VFV30_10280"
     CDS             618..1418
                     /gene="kdsB"
                     /locus_tag="VFV30_10280"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX4848520.1"
                     /translation="MADFAILIPARFASTRYPGKPLAMLRGADGEARSLIERSWRAAT
                     AVAPAEQVWVATDDARIAEAVAGFGGQVVMTPESCRNGTERCAAALEVLGEIAPIIVN
                     LQGDAPLTPAFVVSDLVAALAADPGAAMATPAVECSASLYAHLITDQAAGRVGGTTVV
                     ADAAGRALYFSKRVIPHLPADAPEAHRSVRLHLGVYAYRPAALAAYAALEPTPLELCE
                     GLEQLRFLESGQPIRLVPFPALEWDCIELNNPEDVPAIEGILQARGIR"
     gene            1427..2263
                     /gene="kdsA"
                     /locus_tag="VFV30_10285"
     CDS             1427..2263
                     /gene="kdsA"
                     /locus_tag="VFV30_10285"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX4848521.1"
                     /translation="MTGPTVTLPGGIALANNRPLVLIGGMNVIEDRAVLHEVAAHFVE
                     VTGKLGIPYIFKASFDKANRSSLASFRGPGLEAGLAELAEVKARFGVPVITDVHEPWQ
                     ADAVAQVADILQLPAFLARQTDLVTALARTGKPINVKKPQFLAPEEMRHIIAKCREAG
                     NDQVLLCERGSSFGYNNLVVDMLGIDLMRPLAPVIFDVTHALQKPGGRADSADGRGAQ
                     TAALARAGVAVGIAGLFLEAHPDPAIAKCDGPSALPLHELEPLLRQLTAVDAVIKSGV
                     VA"
BASE COUNT          387 a          792 c          796 g          412 t
ORIGIN      
        1 gtcgctgcgc ggcagaattc gttgctgatc gatctggctg acattgccct gatgctgccg
       61 cgcgtgccgg aggcctgcgc ggtcaacgtg gcaccgacca cgtcgaccac catgcagctg
      121 gcgctaggcg atgctcttgc catggccgtg atggatatgc gcggtgtgtc caagaaccac
      181 ctgcgcgcct tgcatccggc cggtgcgatc gggctggcgc tcgcgccggt tagcgagatc
      241 atgcacgggg ccgatcggct gccgctggtc agccgggatg ccgggatgcc cgaagcgatt
      301 tcggtgatga cctcaggttg ctttggcctg gcgggcgtta ccgatgctga tggcaccctg
      361 gtggggatca tcaccgacgg cgatttgcgc cgcaagctgg gcgtgctgtc caccgccttt
      421 gccggtgaag tcatgacgcc ggcaccgaaa gtgatcccgg ccgaaatgcc ggctggcgaa
      481 gcgctggtct tcctcaacga caacaagatc acggccgcct ttgtggttga ggatccagcc
      541 gcaggcccgc aagcgccgct gggcatcatc catatccacg acctgctgcg acacgggctc
      601 aactgaaccc aaccgccatg gccgatttcg ccatcctgat cccggcccgg ttcgcctcga
      661 cgcgctatcc gggcaagccg ctggccatgc tgcgtggtgc ggacggcgaa gcacgcagcc
      721 tgattgagcg cagttggcgc gcagccacgg cagtcgcccc agccgagcag gtttgggtcg
      781 cgaccgacga tgcgcggatc gccgaagccg ttgcagggtt cggcggacag gtggtgatga
      841 cccccgagag ctgtcgcaac gggactgagc gctgcgccgc tgcgctcgaa gtgctgggcg
      901 agattgcccc gatcatcgtc aacctgcagg gtgacgcgcc gctgaccccg gcctttgtcg
      961 tcagcgatct cgtggcggcg ctcgctgccg atccgggtgc ggccatggcc accccggcgg
     1021 tcgaatgttc ggccagcctc tatgcccacc tgatcaccga tcaggccgcc ggccgggttg
     1081 gcggcacaac cgtggtggcg gatgcggcag gccgggcact gtatttttcg aagcgggtga
     1141 ttccgcatct ccccgccgat gcaccagaag cacatcggtc agtccggctg catctggggg
     1201 tctatgccta tcgcccggcg gccctggccg cctatgctgc gctggagccc accccgctcg
     1261 aactgtgtga ggggctggaa caactgcgct tcctcgaaag cgggcagcca atccggctgg
     1321 tgccgttccc ggcgctggaa tgggactgca tcgaactgaa caatccggaa gatgtcccag
     1381 caatagaagg cattttgcag gcgcgcggca tccgctaaga ggcagcatga ccggtcccac
     1441 cgtaacgctg ccaggcggca tcgccctcgc caacaatcgc ccgctggtgc tgatcggcgg
     1501 gatgaatgtg atcgaggatc gcgcggtgct gcacgaagtg gcggcccact tcgtcgaagt
     1561 gaccggaaag cttggcattc cgtacatttt caaggccagc ttcgacaagg ccaaccgctc
     1621 gtcgctggcc tcgttccgcg gcccggggct ggaagccggc ctggccgaac tagccgaggt
     1681 caaggcccgc ttcggcgtgc cggtaatcac tgacgtgcac gagccatggc aggctgacgc
     1741 ggtcgcgcag gtggccgata tcctgcaact gccggccttc ctggcgcgcc agaccgatct
     1801 cgtgacggcg ctggcccgga ccggcaaacc gatcaacgtc aagaagcccc agttcctcgc
     1861 gcccgaagag atgcgccaca tcatcgccaa gtgccgcgag gccggcaatg accaggtcct
     1921 gctgtgcgag cgcggctcaa gctttggcta caacaacctg gtggtcgaca tgctggggat
     1981 cgatctgatg cggccgctgg ccccggtgat cttcgacgtg acccacgccc tgcagaaacc
     2041 gggcgggcgg gccgacagcg ccgacggccg cggcgcacag accgctgccc tggcgcgcgc
     2101 cggggttgcg gtgggaattg ccggcctgtt tctggaagcc catcccgacc ctgcgatcgc
     2161 gaaatgcgac gggcccagtg ccctgccact gcatgagctg gagccgctgc tgcgtcagct
     2221 gaccgccgtc gatgccgtga taaaatccgg ggtagtggct tgaacgaccc cttcttgcgg
     2281 gttccgccct tccctggcgc acgcggactg ccgctgtgcc ggaccggagc cagcaccgtt
     2341 ccagccgatg aagtcttggc cgcgctggtt gctcatcggg tcggtgg
//
LOCUS       DASUDV010000476         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_237021,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000476 DASUDV010000000
VERSION     DASUDV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237021"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..870
                     /locus_tag="VFV30_10290"
     CDS             82..870
                     /locus_tag="VFV30_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX4848522.1"
                     /translation="MDSPGNSLIFAAAGAALAMTLIVAVRYVLTSGLFAWLTERRMPG
                     LHRRLSPQVRREIGWSLASSAIYGVPAGVLAWGWQERGWTRIYSDVGDYPLWWLPLSF
                     LLCLLLHDTWFYWTHRLMHRPAWFKAMHAVHHDSRPPTAWAAMSFHPLEAITGAVVVP
                     VLVLVLPLHIGVVGLLLAVMTLMGVTNHMGWELFPRWLVHGPAGRWLITASHHQLHHD
                     RYRTNYGLYFRLWDRLCGTDGGLGRFDGKAVANDFGRGQTALPL"
     gene            901..2139
                     /locus_tag="VFV30_10295"
     CDS             901..2139
                     /locus_tag="VFV30_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX4848523.1"
                     /translation="MSLQSDLTGYFMAGITAASAATAMAGRLPKGAARGRTIVLGCGK
                     AAAAMVEVAAAELEGDVTGIVVTRYGHGARQDTSGIEVIEASHPVPDAASLAAGQRIR
                     ALAGQATDRDRVIFLISGGGSALLCDPIAGVSLEEKAAMTAHLVRSGVAIGDINLVRR
                     HLSQVKGGRLAAAARAAAQDMHTFLISDVAGDVPAAIASGPSIACPFEPDRALAVLAA
                     SGWPVSAALAAAVRGAPAVSAPAHPVHLVASGRTALGAVAKAAARDGWTVEVLGDALE
                     GDAASVGRQHAGMALDRARRPGRHLILSGGELTVTVRSPGGRGGPNLEYLTGLMAALP
                     PGASVSALAGDSDGIDGSADNAGGYFDSKHRAAAAECEAALAGNRTYGLFEALGTLVM
                     TGPTRTNVNDIRIIAIEGQP"
     gene            complement(2202..2277)
                     /locus_tag="VFV30_10300"
     tRNA            complement(2202..2277)
                     /locus_tag="VFV30_10300"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2242..2244),aa:Lys,seq:ttt)
     gene            complement(2291..2785)
                     /gene="folK"
                     /locus_tag="VFV30_10305"
     CDS             complement(2291..2785)
                     /gene="folK"
                     /locus_tag="VFV30_10305"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4848524.1"
                     /translation="MGQRYLIALGSNMRHVRHGPPEQVLAAALAALEDAGMTVLAAAP
                     VIRSAPLGPSRRRYANGAALVETGLAPDDLLDTLKAIERRFGRTRGGQRWSSRVLDLD
                     IVLWSGGCWSSPGLTVPHRAFRERTFVLGPAARIAPDWRDPLTALSLRQLHARLTRPR
                     PLPR"
     gene            complement(2789..3640)
                     /gene="aguB"
                     /locus_tag="VFV30_10310"
     CDS             complement(2789..3640)
                     /gene="aguB"
                     /locus_tag="VFV30_10310"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX4848525.1"
                     /translation="MSTLTVAALQLPLGSSDEAENIVAVSMLVEEAAAKGAQLILPPE
                     LFSGPYFCKVEDEALFALSRPTAEHPSVRAMQALAAKLKVAIPTSFFERDGQHYYNTL
                     AMIGPDGAIQGTYRKSHIPDGPGYEEKYYFRPGNDGFKVWDLFGHKVGVGICWDQWYP
                     ECARVMALMGAEVLLYPTAIGSEPYDADLDTSRMWRRAMIGHAVSNCMPVLAANRIGT
                     EDGQRFYGHSFISDEWGDLIAEFGAAETGVLTATLDLARAARHRAGMGFFRDRRPQLY
                     TRIAQDI"
BASE COUNT          583 a         1305 c         1351 g          610 t
ORIGIN      
        1 cggtcggccg ttaagccgtt accccaatct ctctccaaac tcccgttccc aacccgccgc
       61 atctccccta gaccccgccg catggatagc cccggcaatt ccctgatctt tgcagccgcc
      121 ggtgcagcgc tggcgatgac gctgatcgtc gcggtgcgct atgtcctcac cagcgggctg
      181 tttgcctggc tgaccgagcg gcggatgccg gggctgcacc ggcggctgtc gccgcaggtg
      241 cggcgggaga tcgggtggtc gctggcctcc tcggcgatct atggcgtgcc ggccggggtg
      301 ctggcctggg gctggcagga gcgcggctgg acgcggatct acagcgatgt gggggactat
      361 ccgctgtggt ggcttccgct gtcgttcctg ctgtgcctgc tgctgcacga tacctggttc
      421 tactggaccc accggctgat gcaccgcccg gcctggttca aggcgatgca cgcggtccac
      481 catgacagcc gcccgcccac ggcctgggcg gcgatgagct ttcacccgct ggaagcgatc
      541 accggggcgg tggtggtgcc ggtgctggtc ctggtcctgc cgctgcacat cggcgtggtc
      601 ggcctgctgc tggcggtgat gacgctgatg ggggtgacca accacatggg ctgggaactg
      661 tttccgcgat ggctggtcca tgggccggcc gggcgctggc tgatcacggc atcgcaccac
      721 cagctgcacc acgatcgcta ccgcaccaat tacggcctct atttccgcct gtgggaccgc
      781 ctctgcggca ccgatggcgg gctggggcgg tttgacggga aggcggtagc caacgatttt
      841 ggtcgcggcc aaacggcttt gccgctgtaa tcccaccggt cttcggggga gtcgattgcc
      901 atgtcactgc aaagcgatct gaccggctac ttcatggccg gaatcacggc ggccagcgcg
      961 gcgaccgcca tggccgggcg gctgccaaaa ggcgcggcac gggggcggac gatcgtcctg
     1021 ggctgcggca aggcggccgc ggccatggtc gaagtggccg cggcggagct tgagggcgac
     1081 gtcaccggca tcgtggtcac gcgctatgga cacggcgccc gacaggacac cagcgggatc
     1141 gaagtgatcg aggccagcca tccggtgccg gatgcggcca gcctggctgc gggacagcga
     1201 atccgcgccc tggccgggca ggcgacagac cgggaccgcg tcatcttcct gatttcgggc
     1261 ggcggctccg ccttgctgtg cgatccgatt gccggcgttt cgctggagga aaaagcggcg
     1321 atgaccgcgc acctggtgag atcgggcgtg gcgattggcg acatcaacct ggtccgccgg
     1381 catctctccc aggtcaaggg cggccgtctc gccgcggccg cgcgcgcagc ggcgcaggac
     1441 atgcacacct ttctgatttc cgatgtggcc ggcgatgtgc ccgcggccat cgcgtccggg
     1501 ccgtcaattg cctgcccgtt cgaacccgat cgggcgctgg cggttctggc cgcaagcggc
     1561 tggccggttt ccgccgcgct ggcagcggcc gtgcgcggcg ctccggcagt atcagcgccg
     1621 gctcaccccg tccacctggt cgccagcgga cgcaccgcgc tgggcgcggt ggccaaagcc
     1681 gccgcccggg atggctggac ggtcgaggtt ctgggcgacg cgctggaagg cgatgccgcc
     1741 tcggtcggcc gccagcacgc cggcatggcg ctggaccggg cccgccggcc cggccggcat
     1801 ctgatcctct cgggcggcga actgacggtc accgtccgca gccccggcgg ccgcggcgga
     1861 ccgaacctgg aatacctcac cggcctgatg gccgcgctgc cgccgggggc gtcggtttcg
     1921 gctctggccg gggacagcga cgggatcgat ggcagcgccg acaacgccgg tggctatttc
     1981 gattccaagc atcgcgccgc ggcggccgag tgcgaggccg cactggccgg caaccgcact
     2041 tacggcctgt tcgaagccct ggggacgctg gtcatgaccg gcccgacccg caccaacgtc
     2101 aacgacatcc gcattatagc catcgaaggt cagccatgat ccataccgac cggcacgttc
     2161 gccgcgcgcg atccgcaacg cttgtcattt ggcggaagag gtggtgagcc ctgctgggtt
     2221 cgaaccagcg acctactgat taaaagtcag ttgctctacc gactgagcta agggccctca
     2281 ccgaaggcgc tcacctaggg agcggacggg gccgggtcaa gcgcgcgtga agctggcgca
     2341 gcgaaagggc ggtcagcgga tcgcgccagt ccggggcaat ccgcgcggcg gggcccagca
     2401 cgaaggtgcg ctcgcgaaag gcccggtgcg gcacggtcag cccgggcgag gaccagcacc
     2461 cgccgctcca caatacgata tcgagatcga ggacccggct ggaccagcgc tgtccgccgc
     2521 gggtgcggcc gaaccggcgc tcgatcgcct tgagggtgtc cagcagatcg tccggggcaa
     2581 ggccggtctc caccagcgcc gcgccgttgg catagcggcg gcgcgagggg ccaagcgggg
     2641 cggagcggat caccggggcg gcggccagca ccgtcatgcc cgcatcctcc agggccgcca
     2701 gcgcggcggc cagcacctgt tcgggcgggc catggcggac atggcgcatg ttggagccca
     2761 gcgcgatcag atagcgctgg cccacgcctc agatgtcctg ggcgatgcgc gtgtaaagct
     2821 gcgggcggcg atcgcggaaa aagcccatgc cggcgcggtg ccgggcggcg cgggccagat
     2881 cgagcgtcgc ggtcagcact ccggtttcgg ccgcgccgaa ttcggcgatc agatcacccc
     2941 attcgtcgga aatgaagctg tggccgtaga accgctggcc gtcctcggtc ccgatccggt
     3001 tggcggccag caccggcatg cagttcgata cggcgtggcc gatcatcgct cgccgccaca
     3061 tccggctggt atcgagatcg gcatcatagg gctcgctgcc gatcgcggtg ggatagagca
     3121 gcacttcggc ccccatcagc gccatgaccc gcgcgcattc cgggtaccac tggtcccagc
     3181 agatcccgac gccgaccttg tggccgaaca ggtcccagac cttgaacccg tcgttgccgg
     3241 ggcggaaata gtacttttcc tcatagcccg gcccgtccgg gatatggctc ttgcggtaag
     3301 tgccctggat tgccccgtcg gggccgatca tcgccagggt gttgtaatag tgctgcccgt
     3361 cgcgttcgaa gaagctggtc gggatcgcga ccttcagctt ggcggccagg gcctgcattg
     3421 cccggacgga cgggtgctcc gccgtaggcc gggacagcgc gaacagcgcc tcgtcctcga
     3481 ccttgcagaa ataggggccg gaaaacagct cgggcggcag gatcagctgc gcgcccttcg
     3541 ccgcggcctc ctcgaccagc atcgagacgg caacgatgtt ctcggcctcg tccgaggatc
     3601 cgagcggcaa ttgcagggcg gcgacagtca gcgtgctcat gccgccgccc ctagcattcc
     3661 ccgcgccttg cgaaaagcgg gggtgcggat cacacccgct tgcggaacag cagcggcgac
     3721 aactacaccc gcttgcggct cagcagcgga gacaattacg cccgcttgcg gaacagcagg
     3781 gtcatgcggt cgctttcgcc gatcgcctgg aggcgggcct tgtcttcctc cttggcgccg
     3841 cgcatggcc
//
LOCUS       DASUDV010000477         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1363961,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000477 DASUDV010000000
VERSION     DASUDV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363961"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFV30_10315"
     CDS             complement(<1..1576)
                     /locus_tag="VFV30_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848526.1"
                     /translation="MSLALNEARPSAVEPPPVAAGLASSVGSTVPAASLPATQAAPPA
                     ARPAVPSLAAVFAPTSHVQPASVAPEPSKQPQGAAFAVPVPVANGVMGIGAGPGPPPG
                     EAAAGLVRPANLQQIAGGAPPDISDDDELILELRLANGMLADTLVGYGTRNGIYLPLG
                     ALARSLDLAIVVSDDGNYASGWFLDEKRTLTIDLRQKRLELLGKSRDFPSGAFVAREG
                     EMYVLAEYLGELLPLTLKADLRDQSVTITTREPFPFEARIAREEARGRLGNQNATLAE
                     VFQRERTDWRLIDFPLVDLELRGVSDRVLGTRAEGDVRLSTDLAYMNAQTFFSASSKD
                     GLTAARIELGRQDPDGTLLGPLGATEFQIGDVSTTALPIGLRGTSGRGAFVSNARLYR
                     ASVFDKIDLRGELPDGYEVELYRNNVLIGSTRTAINGRFEFLQVPVDFGLNVMRLVFY
                     GPQGQRREEVRQIAVGDGRLAEGEFEYSVGMAQKERNLLNIYGPFFKPTRDFGAWRMT
                     GQMSYGFTKDITGTLSF"
     gene            2253..2474
                     /locus_tag="VFV30_10320"
     CDS             2253..2474
                     /locus_tag="VFV30_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848527.1"
                     /translation="MNLPFSPSLGLGKGLAIPCFAGSGPGSGSGSVRSTITPSQAVSL
                     RSSNAIPKASVPALIRLVVAWRDPSVTAP"
     gene            complement(2461..3336)
                     /locus_tag="VFV30_10325"
     CDS             complement(2461..3336)
                     /locus_tag="VFV30_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848528.1"
                     /translation="MIALAWPISLGAQSAPDSAANAPTGSVGGLGDINLFPKRVVLDG
                     RVRVASIGIYNKTINTGDYDIRVSDRVMNEDGQLVDLAAVTDPALKARVKTASAFLRW
                     SPRKVTLRGSESQTVRVMARITPDLAPGEYRSHFQAIAIPPGGDGGTSIDQARNRESG
                     TGIGVRILPRFGISIPIIVRVGTTTLTTGLKDLRLAATPDGQPALRFTITRDGNRSAF
                     GNIVVTMAGAKQPLAEIKGIGVYPEITQRHVLVPIDPAALAAPLRSGTKVTVTYIDDD
                     FAPGKILARQDFTVP"
BASE COUNT          582 a         1107 c         1224 g          628 t
ORIGIN      
        1 cgaacgagag cgtgccggtg atatccttgg tgaagccata ggacatctgc ccggtcattc
       61 gccaggcgcc gaaatcgcgg gtgggcttga agaacgggcc gtagatgttg aggaggttgc
      121 gctccttctg cgccatgccg acggagtatt cgaattcacc ttccgccagg cggccatcac
      181 caaccgcgat ctggcgcact tcctcgcggc gctggccctg cggaccatag aagaccagcc
      241 gcatcacgtt gagcccgaaa tcgaccggaa cctgaaggaa ttcgaaccgg ccgttgatcg
      301 cggtacgggt cgatccgatc agcacgttgt ttcgatagag ttccacctcg tagccatcag
      361 gcagctcgcc gcgcaggtcg atcttgtcga acaccgaggc gcggtagagc ctggcattgg
      421 aaacaaaggc gccgcgtccc gatgtgccgc gcaggccaat cggcaaggcc gtggtcgaaa
      481 catcgccgat ctggaattcg gttgcgccca gcggtccgag cagcgtgccg tcgggatcct
      541 ggcggcccag ctcgatccgc gccgccgtaa gcccgtcctt gctagacgcg ctgaaaaagg
      601 tctgcgcgtt catataggcc agatcagtcg aaagcctgac atcgccttcg gcgcgggtgc
      661 caagtacccg gtccgaaaca ccgcgcagtt ctaggtccac cagcgggaag tcgatcagcc
      721 gccagtcggt ccgctcgcgc tgaaacactt cggcaagggt ggcgttctgg ttgccaagcc
      781 ggccgcgtgc ttcctcgcgg gcgatccggg cctcgaatgg gaacggttcg cgggtggtga
      841 tcgtgaccga ctggtcacgc aggtcggcct tgagggtcag cggcaataat tcgcccaggt
      901 attcggccag cacgtacatt tcgccttcgc gcgcgacgaa agccccggaa ggaaaatcgc
      961 ggctcttgcc aagcaactcg agccgctttt gccgaaggtc gatggtcagg gtgcgctttt
     1021 cgtcaaggaa ccagccgctg gcataattgc catcgtcgct gaccacgatt gccagatcga
     1081 ggctgcgtgc cagcgcaccc agcggcaggt aaatgccgtt gcgcgttcca tagccgacca
     1141 gcgtgtcggc cagcatgccg ttggcgagcc tgagttcgag gatcagctcg tcgtcatcgg
     1201 agatgtcggg cggggcgccg ccggcgatct gttgaaggtt ggctggacga accagaccgg
     1261 cggcggcttc accgggtggc ggccccggac cggcaccgat ccccattacc ccgttcgcca
     1321 ccggcacggg tacggcaaag gcggcgcctt gcggctgctt cgaaggttcg ggtgcgaccg
     1381 aggcgggctg gacatggctg gttggcgcga agaccgcggc aagcgagggc actgccgggc
     1441 gcgcggccgg cggcgctgct tgcgtggcag gaagcgaggc ggccggaacc gtgctgccga
     1501 ccgagctggc aagtccggcg gcaaccgggg gtggttcgac cgcgctgggg cgggcctcgt
     1561 tcagcgcgag cgacaccggt gcggcttctg gaaccctggc ttccgcggca ggcgcggcca
     1621 ctggcggctg tggttgagcc ggctcgggca ttgcggcggg gcttgccgcg atttgccgcg
     1681 tcggtgtcgg agccgcctgg acggtttcaa ccggttggac tgtgggcggc agcggtgcag
     1741 gcgccggctg gaccttcggc gcaaccggga cggccagcgc gataggtgcg gactgcaccg
     1801 ggctttgctc aacctgggct gcttgtgctt gcactgccgg gatttcagaa gcggcggctg
     1861 gtgccgcggt atcagccttg ggcgcggcga gaggttcggc gggcgtgtct tgcgccagcg
     1921 gctgctgcgc tgctggcccc ggagcgacca gattgactgg cgctgccggt tcgggctggt
     1981 cggcttgcgg gaccgagggc tggggcagag gcccaggccc aggcggggga accgctgggg
     2041 cttgggcaga caggctgggc gtggggaaag ccgcgcgttc gatgccgggc gcgggagggt
     2101 ttgtctgggc aaatgttccg ctcagcgccg aactggcttg ggccttggcc ttgatcccgc
     2161 gagggccaat cggttccgcg cgcacccgtt tcgggccaag cagggtttca ctcaggcttg
     2221 gcacgcgcac cggcgcgtgc agggccgggc cggtgaactt gcctttttcg ccaagcctgg
     2281 ggctgggcaa gggcttggca ataccgtgct ttgccggatc ggggcccggc tctggctccg
     2341 gatcggtcag gtcgacgatc accccgtccc aggccgtatc gctgcgcagc agcaacgcga
     2401 tcccgaaggc gagcgtcccg gctctgatcc gtctggttgt ggcctggcgt gaccccagtg
     2461 tcacggcacc gtgaagtcct gcctcgccag gatcttgccg ggcgcgaagt cgtcatcgat
     2521 gtaggttacg gtaacctttg tgccagaccg cagcggcgcg gcgagggccg cgggatcgat
     2581 cggtacaagc acgtggcgct gggtgatttc gggatagacg ccgatcccct tgatctcggc
     2641 caagggctgc ttggcaccgg ccatggtcac cacaatgttg ccgaaggctg accggttgcc
     2701 atcgcgggtg atcgtgaaac gcaaggcagg ctgcccatcg ggcgttgccg ccaggcgcag
     2761 gtccttgagg cccgtggtca gcgtagtggt gccaaccctg acgatgatcg ggatcgaaat
     2821 gccgaagcgc ggcaggatcc gcaccccgat ccccgttccg ctttcgcgat tgcgcgcctg
     2881 atcgatcgac gtcccgccat cgccccccgg cgggatggct atggcctgga aatgcgaacg
     2941 atattcgccc ggtgctagat cgggggtgat ccgtgccatg acccgcacgg tctgcgattc
     3001 actgcccctc agcgtgacct tgcgcggcga ccagcgcaaa aaggcgctgg cggtcttgac
     3061 ccgggccttg agcgcgggat cggttaccgc cgccagatcg accagctggc cgtcctcgtt
     3121 catcacccgg tcggatacgc ggatgtcgta atcgccggtg ttgatggtct tgttgtagat
     3181 gccgatcgag gccacccgga cccgcccgtc aagcaccacc cgcttcggaa acaggttgat
     3241 atcgccaagt ccgccaaccg atccggtggg agcattcgct gcgctgtcag gagcggactg
     3301 ggcgccaagc gagatcggcc atgccagcgc gatcaagaac gccccggcaa ccggcaagga
     3361 tcttgcggcc ccaagcgccg ctgaatcccg tcgattcatt gaattgtctc cgccttcgaa
     3421 cagcctttgg ccagttcaat aggcgaatac acctaagcgg tgcaatcagg ccctactggt
     3481 aaatcacagt tacgtcgaag gtacccgaat agttgccgcc catctggttt gcggcaacgt
     3541 t
//
LOCUS       DASUDV010000478         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_104606,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000478 DASUDV010000000
VERSION     DASUDV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104606"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..553
                     /gene="ppa"
                     /locus_tag="VFV30_10330"
     CDS             14..553
                     /gene="ppa"
                     /locus_tag="VFV30_10330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX4848529.1"
                     /translation="MRIDLIPAGPNPPEELNVLIEVPIGGEPVKYEFDKASGALFVDR
                     ILHTPMRYPANYGFVPHTLGEDGDPLDALVITRSPILPGAVIRVRPVGVLLMEDDKGG
                     DEKLICVPVDATFPYYREVAGHEDLPPIVTQQIEHFFQHYKDLEPGKWAKLNGWGDAD
                     QAKQIIVECIERYKATAAR"
     gene            complement(576..1919)
                     /locus_tag="VFV30_10335"
     CDS             complement(576..1919)
                     /locus_tag="VFV30_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX4848530.1"
                     /translation="MLSPHAAQDRAAALIAFARAAGADAADAVYSGESSESVQVRLGQ
                     LEDVERSESEHLDLRVFVGSRSASIGSSDLSDAALKELASRAVDMARAAPEDRFAGLA
                     PEELLTSGPFDAFDMTDPVELSPAALRERAREAEEAALAVPGVTNSEGGQASLAQRVF
                     ALVTSHGFAAARSGTMHGISATAIAGEGGGMQRDYAWRMAHHLEDLPPLAEIGALAGT
                     RAVGRLEPGRVKSGAMPVVFDPRVGGSLLSHLAGAMSGNAIARRASFLLGREDEQLFA
                     PGVAVFDDPHRSRGLSSRAFDGEGLATAPRALVEDGRLTGWLMDSAAARQLGGTPTGH
                     AVRGGGAPGVGTSNLHLAAGTASVAELIADIAEGVLVTELIGHGVNPVTGDYSRGASG
                     LRIVNGQLAGPVAEFTVAGNLIDMYRNLVAANDLEWHRVVNVPTIRIDGMTVAGD"
     gene            complement(1984..2364)
                     /locus_tag="VFV30_10340"
     CDS             complement(1984..2364)
                     /locus_tag="VFV30_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848531.1"
                     /translation="MAKAWEGTAQATRHPVKALDWRKRMSDNIAYALLVYTGLQIFVT
                     MGALDTKGGSILPYFTLIVLVVAIIPACRRFEKRWNRLSDVQAADPQFVRFYRRDQIW
                     LWAMAIGLPFVVYGLFAGMARLLA"
     gene            2542..3321
                     /gene="lptB"
                     /locus_tag="VFV30_10345"
     CDS             2542..3321
                     /gene="lptB"
                     /locus_tag="VFV30_10345"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX4848532.1"
                     /translation="MSDVQHLVPPNTQAMQPIPQGGLEVISIAKSYDKRAVLTDISLS
                     VGKGEVLGLLGPNGAGKTTCFYSIMGLVRPDSGRILMDGVDVTNLPMYRRAILGLGYL
                     PQETSIFRGLTVEQNIAAVLELAEPDKATRERELQRLLDEFGLDRLRSSPAMALSGGE
                     RRRCEIARALAANPSIMLLDEPFAGIDPLSISDIRDLVKDLKQRGIGVLITDHNVRET
                     LDIVDRACIIYGGQVLFAGSPEELVADENVRRLYLGEGFTL"
     gene            3332..>4272
                     /locus_tag="VFV30_10350"
     CDS             3332..>4272
                     /locus_tag="VFV30_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX4848533.1"
                     /translation="MALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLEIEAFIGEALE
                     ANPLLDLGSGGGDAEAPPPEPDDLRRTTLESSPADQLIAEGRAGEDRPLDVDPTALDR
                     DRDTGDWSADFRGPPGEDGPGIDERGAGDPTLAEHLHAQIGEITRDPRLAFLARHLAS
                     ELDEAGYLAATLGELAAELGVDEDELEEALALVQSLDPVGVGARSLGECLALQAKEAD
                     RYDPCMAKLLDNLDLLARGELPRLKRMCGVDDEDFADMLAELRGYDPKPGLRFAGGGG
                     DPVTPDILLTPRADGGWDVALNQATLPRLVINRSYYV"
BASE COUNT          716 a         1492 c         1435 g          629 t
ORIGIN      
        1 ctaagggcgc gccatgcgta ttgaccttat ccccgccggg cccaacccgc ccgaagagct
       61 gaacgtcctg atcgaagtgc cgatcggcgg cgaacccgtg aagtacgaat tcgacaaggc
      121 gtcgggcgcg ctgttcgtcg accggatcct gcacaccccg atgcgctatc cggccaacta
      181 cggctttgtc ccccacacct tgggcgaaga cggcgatccg cttgacgcgc tggtgatcac
      241 ccgctcgccg atcctgcccg gcgcggtgat ccgggttcgc ccggtcggcg tgctgctgat
      301 ggaagacgac aagggtggcg acgaaaagct gatctgtgtc cccgtcgacg cgaccttccc
      361 ctattaccgc gaagtcgccg gccacgagga cctgccgccg atcgtgaccc agcagatcga
      421 gcacttcttc cagcactaca aggatctgga acccggcaag tgggccaagc tcaacggctg
      481 gggcgatgcc gatcaggcca agcagatcat tgtcgagtgc atcgaacgct acaaggcgac
      541 ggccgcgcgc tgatcccgcc cgtgggcggg cggcgtcaat cgcccgccac ggtcatcccg
      601 tcgatccgga tcgtcggcac gttgaccacc cggtgccatt ccagatcgtt tgccgccacc
      661 aggttgcggt acatgtcgat caggttgccg gccacggtga attccgccac cggcccggcc
      721 agctgcccgt tcacgatccg caggcccgaa gcgccgcggc tgtaatcgcc ggtcaccggg
      781 ttcaccccgt ggccgatcag ttcggttacc agcacccctt cggcaatatc ggcaatcagc
      841 tctgccacgc tggcggttcc ggcggcgagg tgcaggttgc tggtgcctac ccccggcgcg
      901 ccgccgccgc gcacggcatg gccggttggc gtgccgccca gctggcgcgc ggccgcgctg
      961 tccatcagcc agccggtcag ccgtccgtcc tcgaccagcg cgcgcggcgc cgtggccagt
     1021 ccttcgccat caaaggcgcg ggaggaaagc ccgcgcgagc ggtgcggatc gtcgaacacc
     1081 gccacgcccg gcgcgaacag ctgttcgtcc tcgcggccga gcaggaagct cgcccggcgg
     1141 gcaatcgcgt tgcccgacat ggcgccggcg aggtgcgaga gcagcgagcc gccgacccgc
     1201 ggatcgaaca ccaccggcat ggccccgctc ttgacccggc ccggctccag ccggcccacg
     1261 gcgcgcgtgc cggccagggc gccgatctcg gccagcggcg gcaggtcttc cagatggtgc
     1321 gccatccgcc aggcatagtc gcgctgcatg ccgccgcctt cgccggcgat cgcggtggcg
     1381 ctgatcccgt gcatggtccc gctgcgcgcg gcggcgaaac cgtggctggt caccagggca
     1441 aagacccgct gcgccaggct ggcctgcccg ccttcggaat tggtcacccc gggcacggcc
     1501 agcgcggcct cttccgcctc gcgggcccgc tcgcgcaggg cggcgggcga aagctcgacc
     1561 ggatcggtca tgtcgaaagc atcgaagggg ccggaggtga gcagctcttc gggcgcaagg
     1621 ccggcaaagc ggtcttccgg cgcggcgcgg gccatgtcga ccgcgcgcga ggccagctcc
     1681 ttcagcgcgg catcggacag gtcgctcgat ccgatcgagg cggaacggct gccgacgaag
     1741 acccgcaggt cgaggtgttc actttccgaa cgctcgacgt cttccagctg gcccagccgg
     1801 acctgcacgc tttccgacga ttcgccggaa tagaccgcat cggcggcatc ggccccggcg
     1861 gcgcgagcga aggcgatcag cgcggcggcg cggtcctggg cggcgtgggg actgagcatg
     1921 gaaacggcga ctccggacag aaagcgggag ccgccgatct agggactaaa gcggcaaaag
     1981 caatcaggcg agcaggcgcg ccatgccggc gaacaggcca tagaccacga agggcaggcc
     2041 gatcgccatg gcccacagcc agatctgatc gcggcggtag aaccgcacga actggggatc
     2101 ggcggcctgg acgtcgctca gccggttcca gcgcttttca aagcggcggc aggccgggat
     2161 gatcgccacg accagcacga tcaacgtgaa gtagggcagg atcgatccgc ccttcgtgtc
     2221 gagcgcgccc atggtgacga agatttgcag accggtatag accagcagcg cataggcgat
     2281 gttgtcgctc atccgcttgc gccaatcgag cgccttgacc ggatgccgtg ttgcctgtgc
     2341 ggttccttcc caggcttttg ccatggttcc accccttttc agttcccctc aggacgtgag
     2401 tagaacaccg aagggcccgg ggatcaagag ccgttaagca ttgtttgcca caagcctgct
     2461 tgcggcagcc atgcacgaaa cctgccaaat cgaccaacgg agcttgcggg ccggcagccg
     2521 cccgcctaga tcaaggggcc tatgagcgac gttcaacacc tcgtcccgcc caacacccag
     2581 gccatgcagc ccatcccgca gggcggcctg gaagtgatct cgatcgccaa gagctatgac
     2641 aagcgggcgg tgctgaccga catttcgctg tcggtcggca agggcgaagt gctgggcctt
     2701 ctgggcccca acggagccgg caagaccacc tgcttctatt cgatcatggg gctggtgcgc
     2761 ccggattcgg gccggatcct gatggacggg gtcgatgtca ccaacctgcc gatgtaccgc
     2821 cgggcgatcc tgggcctggg ctatctgccg caggaaacca gcatcttccg cgggctgacc
     2881 gtggaacaga acatcgccgc cgtgctggag ctggccgagc cggacaaggc cacgcgcgag
     2941 cgtgagctgc agcgcctgct cgatgaattc gggctcgacc ggctgcgctc cagcccggcc
     3001 atggcgctgt ctggcgggga acgccgccgc tgcgagattg cccgcgcgct ggccgccaac
     3061 ccgtcgatca tgctgctgga cgaacccttc gcgggcatcg acccgctgtc gatcagcgac
     3121 atccgcgatc tggtgaagga cctgaagcag cgcggcatcg gcgtgctgat caccgatcac
     3181 aacgtccgcg aaacgctcga catcgttgac cgcgcctgca tcatctacgg cggccaggtg
     3241 ctgttcgccg gcagcccgga agagctggtg gccgacgaga acgtgcggcg gctctacctg
     3301 ggcgaagggt tcacgctcta ggccccgatc gatggccctc ggcccgcgcc ttgacctacg
     3361 gcaatcccaa tcgctggtga tgacgcccca gctccagcag gcgatcaaac tgctggcgct
     3421 gtccaacctg gagatcgagg ccttcatcgg cgaggcgctg gaagccaacc cgctgctcga
     3481 tctgggcagc ggcgggggcg atgccgaagc gccgccgccc gaaccggacg acctgcgccg
     3541 cacgacgctt gaaagctccc ccgccgacca gctgatcgcc gaaggccggg ccggcgagga
     3601 ccgcccgctc gatgtcgacc cgaccgcgct tgaccgggac cgtgacaccg gcgactggag
     3661 cgccgatttc cgagggccgc cgggcgagga cggccccggg atcgacgaac gcggggccgg
     3721 cgatccgacc ctggccgagc atctccacgc gcagatcggc gagatcaccc gcgatccccg
     3781 gctggccttc ctggcccggc acctggccag cgagctggac gaggccggct atcttgccgc
     3841 caccctgggc gagctggcgg cggaactggg ggtcgacgag gacgaactgg aagaggcgct
     3901 ggcgctggtc cagtcgctcg atccggtcgg ggtcggcgcg cgcagcctgg gcgaatgcct
     3961 ggcgctgcag gccaaagagg ccgaccgcta tgacccctgc atggccaagc tgctcgacaa
     4021 tctcgatctc ctcgcccgcg gggagctgcc ccggctgaag cggatgtgcg gggtggatga
     4081 cgaggacttt gccgacatgc tggccgagct gcgcggctac gaccccaagc cgggtctgcg
     4141 ctttgccggc ggcggcggcg atccggtcac gcccgatatc ctgctgaccc cgcgcgccga
     4201 cggcggctgg gacgtggcgc tcaaccaggc aaccctgccg cggctggtga tcaaccggtc
     4261 ctactatgtc ga
//
LOCUS       DASUDV010000479         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1849966,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000479 DASUDV010000000
VERSION     DASUDV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849966"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFV30_10355"
     CDS             complement(<1..417)
                     /locus_tag="VFV30_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX4848534.1"
                     /translation="MKATQKDFKALAAKAAREARVFFFCGPDEAGANDAAHVIAGYLQ
                     NPGERIELSGAELKRDPVRLGDEARSTSLFGESRHIWIRASGDEVHDAVANLIEGEVE
                     GCPVLIVATGATDKSRTAKLLAARPDALVAMFWPPDL"
     gene            complement(414..905)
                     /gene="lptE"
                     /locus_tag="VFV30_10360"
     CDS             complement(414..905)
                     /gene="lptE"
                     /locus_tag="VFV30_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX4848535.1"
                     /translation="MRLIVLAFALMLSACGLKPMYSGGAGGTVAQGLAGIEVSPIEGK
                     SGWLMRNALIDRLGAGAAKGNPRYRLDVRLDDKLEGLGLLSDDTIARERRTLRARYQL
                     IDLATGEIVLDATAGSDAGIDVVSSEYATVAAENTALENLTREVADRIVTRVALTLRN
                     AKP"
     gene            complement(905..>3043)
                     /gene="leuS"
                     /locus_tag="VFV30_10365"
     CDS             complement(905..>3043)
                     /gene="leuS"
                     /locus_tag="VFV30_10365"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX4848536.1"
                     /translation="YYGHEQALFLDMYEAGLVYRKESAVNWDPVDMTVLANEQVIDGR
                     GWRSGALVEKRKLSQWFLKITQFADDLLDGLGALKNWPEKVRLMQENWIGKSQGLRCT
                     FRFVDSPDGIEVYTTRPDTMFGASFVAIAADHPFAQAIAANAPELAAFIAECKQGGTT
                     AAELETAEKKGFFTGYSVEHPLKKAWHLPVYVANFVLMDYGTGAVFGCPAHDQRDLDF
                     ARKYELPVTRVVSDGDLTDAEFDGDEANTGPGRIVNSEWLDGMTIDEAKAAVIAKAAH
                     EGWGQGETQYRLRDWGVSRQRYWGTPIPFIHCDACGVVPVPKDQLPVVLPEDVSFDVP
                     GNPLLRHPTWKHVDCPQCGKPAVRETDTLDTFADSSWYFLRFASQPGDKPFDPEVIKQ
                     WLPVEQYIGGIEHAILHLLYARFWTRALNHIGMIEAKEPFASLFTQGMVTHETYSRDA
                     DGRPVYYSPEEVIRSGDGATLKADGQPVTIGKVIKMSKSKKNVVDPDDIIAQYGADAV
                     RWFMLSDSPPERDLPWSEAGIEGCARFVQRLWRLFGQVGLGGAGADAEHDAALERKVH
                     QTIAAVASDIEALSFNKAVARIYELTGAIEKAAPSASRSEAIRTLIRLVAPMMPHLAE
                     EAWSRFGRGLVAEAAWPQHDPALLVEDEVTIAIQVLGKLRDTVTVAKGLPQAELEALA
                     LASEKVQRALDGKPVRKVIVVPDRLVNLVA"
BASE COUNT          460 a         1068 c          992 g          523 t
ORIGIN      
        1 caggtccggc ggccagaaca tcgccaccag cgcgtcaggc cgcgcggcca gcagcttggc
       61 agtgcgcgac ttgtcggttg cgccggtcgc cacgatcagc accgggcagc cttcaacctc
      121 gccctcgatc aggttggcca ccgcgtcgtg cacttcatcg ccgctggcgc ggatccagat
      181 gtgccggctt tccccgaaca gcgaggtgga gcgcgcctcg tcccccagcc gcaccgggtc
      241 gcgcttcagt tcggcgccgc tcagctcgat ccgttcgccg gggttctgca gatagccggc
      301 gatgacatgg gcggcgtcgt ttgcgccggc ctcgtccggc ccgcagaaga agaacacccg
      361 ggcctcgcgc gcggccttgg cggcaagggc cttgaagtcc ttctgggtcg ccttcacggc
      421 ttggcgttgc gcagggtcag ggccacgcgg gtgacgatcc ggtcggccac ttcgcgggtc
      481 aggttttcca gcgccgtgtt ctcggccgcg accgtggcgt attcgctgct gaccacgtcg
      541 atcccggcgt cggacccggc cgtggcgtca agcacgatct cgcccgtggc cagatcgatc
      601 agctgatagc gggcgcgcag ggtgcggcgc tcgcgcgcga tggtatcgtc ggacagcagg
      661 cccaggcctt ccagcttgtc atccagccgc acgtccagtc ggtagcgcgg attgcccttc
      721 gctgccccgg cgcccagccg gtcgatcagc gcgttgcgca tcagccagcc ggacttgccc
      781 tcgatcggcg aaacctcgat tcccgccagg ccctgcgcca ccgtgccgcc ggccccgccg
      841 gaatacatcg gcttcagccc gcaggcggag agcatcaggg caaaggcaag aacgatcagc
      901 cgcatcacgc caccaggttc accaaacggt cgggcacgac gatcaccttg cggaccggct
      961 tgccatccag cgcacgctgg accttctcgc tggccagcgc aagcgcctcc agctcggcct
     1021 ggggcaggcc cttggcgacg gttaccgtat cgcgcagctt gcccaacacc tggatcgcga
     1081 tggtcacctc gtcctcgacc agcagggccg ggtcgtgctg gggccaggcg gcttcggcga
     1141 ccaggccccg gccgaaccgc gaccacgctt cctcggccag gtgcggcatc atcggcgcaa
     1201 ccaggcggat cagcgtgcgg atcgcctcgg accggctggc cgagggcgcg gccttttcga
     1261 tcgccccggt cagttcatag atccgcgcca ccgccttgtt gaaggacagc gcctcgatat
     1321 cgctggccac cgccgcgatg gtctggtgga ccttgcgctc cagcgcggca tcgtgctccg
     1381 cgtcggctcc ggccccgccc aggccaacct gcccgaacag tcgccacagc cgctggacga
     1441 accgggcgca gccttcgatc ccggcttcgg accagggcag gtcgcgctcc ggcgggctgt
     1501 cggacagcat gaaccagcgg accgcgtcgg cgccgtactg ggcaatgatg tcgtccggat
     1561 cgacgacatt cttcttgctc ttcgacatct tgatgacctt gccgatcgtg accggctggc
     1621 catcggcctt cagcgtggcg ccatccccgc tgcggatcac ctcttccggg ctgtaataga
     1681 ccggccggcc atcggcgtcg cgcgaatagg tttcgtgcgt caccatgccc tgcgtgaaca
     1741 ggctggcgaa gggctccttc gcctcgatca tgccgatgtg attcagcgcg cgggtccaga
     1801 accgggcata gagcaggtgc aggatcgcgt gctcgatccc gccgatgtac tgctccaccg
     1861 gcagccactg cttgatcact tcgggatcga acggcttgtc gcccggctgg ctggcaaagc
     1921 gcaggaagta ccagctggaa tcggcaaagg tatccagcgt gtcggtctcg cgcacggccg
     1981 gcttgccgca ctgcgggcag tcgacgtgct tccaggtcgg gtggcgcagc agcgggttgc
     2041 cgggcacgtc aaagctcacg tcctcgggca gcaccactgg cagctggtcc ttgggcaccg
     2101 gcaccacgcc gcaggcgtcg cagtggatga aggggatcgg cgtgccccag tagcgctggc
     2161 gcgaaacgcc ccagtcgcgc aggcggtact gcgtctcgcc ctggccccag ccttcgtgcg
     2221 cggccttggc aatgacagcg gccttggcct cgtcgatggt catcccgtcg agccattccg
     2281 aattcacgat ccggcccggg ccggtattgg cctcgtcgcc gtcgaactcg gcatcggtca
     2341 ggtcgccgtc gctgaccacg cgggtcaccg gcagctcgta cttgcgggca aagtcgaggt
     2401 cgcgctggtc gtgcgccggg cagccaaaga ccgcgccggt gccgtagtcc atcagcacga
     2461 agttggcgac atagaccggc aggtgccacg ccttcttgag cggatgctcc accgaatagc
     2521 cggtgaagaa gcccttcttt tcggcggttt ccagctcggc cgcggtagtt ccgccctgct
     2581 tgcactcggc aatgaaggcg gccagctcgg gcgcgttggc ggcgatcgct tgcgcaaagg
     2641 gatggtccgc cgcaatcgcg acaaagctgg cgccgaacat cgtgtcgggc cgggtcgtgt
     2701 agacctcgat cccgtccggg ctgtcgacga agcggaaggt gcagcgcagg ccctggctct
     2761 tgccgatcca gttttcctgc atcagccgga ccttctcggg ccagttcttg agagcgccca
     2821 ggccgtccaa caggtcatcc gcgaactggg tgatcttgag gaaccactgg ctcagcttgc
     2881 gcttttcgac cagcgcgcca gagcgccagc cgcgcccgtc gatcacctgt tcgttggcca
     2941 gcacggtcat gtcgaccggg tcccagttga ccgccgattc cttgcggtag accagccccg
     3001 cctcgtacat gtccaggaac agcgcctgtt cgtggccgta ata
//
LOCUS       DASUDV010000480         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1827993,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000480 DASUDV010000000
VERSION     DASUDV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827993"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFV30_10370"
     CDS             complement(<1..387)
                     /locus_tag="VFV30_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX4848537.1"
                     /translation="MNGLIGHEQAWREWDEALGSARMHHAWLLAGPKGLGKGLFAQAS
                     AACLVAEPGLTQPDPEHHPDIIVLDHLPANETEEEKKAEGKPYQTRRNITIEQVRRMQ
                     HRLTTRPTLGSRRAVIIDPADDLEKNA"
     gene            complement(384..1019)
                     /gene="tmk"
                     /locus_tag="VFV30_10375"
     CDS             complement(384..1019)
                     /gene="tmk"
                     /locus_tag="VFV30_10375"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX4848538.1"
                     /translation="MSRGKFIAFEGGEGAGKSTQARLLERALDARGITALVTREPGGT
                     PGAEAIRALLLGTDGEGWHPRAEALLFAAARSDHVERLIQPALEAGKWVICDRFVDSS
                     RAYQGGAGGLSDTDVLELHRIGSGGLLPDLTLLIEVSPAVAMARTAVRDGDNPDRIGG
                     RDAAYHASVAKAFSRFAEDEPARFARIDGDGTAEQVHQAVLTALLDAGLLA"
     gene            complement(1016..2092)
                     /locus_tag="VFV30_10380"
     CDS             complement(1016..2092)
                     /locus_tag="VFV30_10380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX4848539.1"
                     /translation="MPPPTTRTPIGLLVDLSAGQTLYARNAEARMIPASMTKAMSALV
                     AFDLIKAGKLRESDMVTVRPQTAARWAGKGTSLNLRAGEQVSVHDLLRGLNTVSANDA
                     AVVLAEAALGSEAAWTRAMNRRAQELGMNASHFATSNGWPDGGKTYVTARDMVRPAQA
                     LIVEHPELYRRYIGRKSFAWRGAVLTSHDPLAEILPGADGIKTGFTSQAGYTFLGAVQ
                     REGRRLILVIGRAPSESARALAATELAEWGYARWDSRPFLDRSWIVGAARVQDGAVRQ
                     VPLSVARPFALTVPKGTKPPVAGRIIYSGPLRAPIGKGTVVAGLQVHMQGQPDHFLPL
                     VATRDVARAGPIDRLRNGLLGLLE"
     gene            complement(2230..3126)
                     /locus_tag="VFV30_10385"
     CDS             complement(2230..3126)
                     /locus_tag="VFV30_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4848540.1"
                     /translation="MPKNGPAADYPIVVGAPYVVNGTNFTPVDTMNYDAVGRASVSPE
                     GGSAVSGAHHTLPLPSYVEVTALDSGRTILVRVERRGPMNGTQLIELSPGAAAQLGMV
                     GTVQAAVRVRRVNPVEPERALLRAGQSAPARMDTPKSLLIVLQRKLDAQEGVVRAPVQ
                     ADPEPATTPKVDQPTQAKPAAPAKPKPEPKKPAPKKPEVKKPEAKPAEAPKPAVQAPA
                     EPKPGAKQPQSAGRFLVQVGAYSSKANADAAARKSGGSANQSGKLWSVRMGPFASEAD
                     AKAALARARAAGYSDARILRSN"
     gene            complement(3212..4318)
                     /locus_tag="VFV30_10390"
     CDS             complement(3212..4318)
                     /locus_tag="VFV30_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX4848541.1"
                     /translation="MSLPHAAKLIAPLLLATSLLSSSIAQEAPPTDLAQSATIAAPPA
                     GSEAPAAVPSVDAGFAGYLQLIAARARSEGVSEETIQSVLSGLTYNPRVVALDRSQPG
                     STPNSPPAFWPYYNRTISAARIESGRARLADTRDITSSIEAEYGVPAKMLIAIWGKES
                     FYGRVQGDFDLPRSLATLAYEGRRRELFEGELVAVLKMVDRGVPRQKLVGSWAGAFGN
                     PQFLPSVYLRLARDGDGDGLADIWASQRDTLVSIANYFRDAGWRPGEPWGVRAQVPAQ
                     FDRSGFKAELEAPTCPRVHNRHSQWKTVAEWRSLGIIPQAPIADTTLATLFEPDGPGN
                     GSFLLTGNYRVILHYNCSNYYALSVGLLADEIAR"
     gene            4404..4493
                     /locus_tag="VFV30_10395"
     tRNA            4404..4493
                     /locus_tag="VFV30_10395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4438..4440,aa:Ser,seq:tga)
BASE COUNT          757 a         1509 c         1404 g          823 t
ORIGIN      
        1 ggcgttcttt tccagatcgt cggccggatc gatgatcacc gcgcggcggc tgcccagcgt
       61 cggccgggtg gtgagccggt gctgcattcg ccggacctgt tcgatcgtga tattgcgcct
      121 ggtctgatag ggtttgccct cggccttctt ctcctcctcg gtttcattgg ccggcaggtg
      181 atcgagcacg atgatatcgg ggtggtgttc gggatctggt tgcgtcaggc cgggttcggc
      241 caccagacag gccgccgagg cctgggcgaa cagccccttg cccagcccct tgggccccgc
      301 cagcagccag gcgtggtgca tccgcgccga tcccaaggct tcatcccatt cgcgccaggc
      361 ctgttcgtgg ccgataagac cgttcatgcc aacaggcccg catccagcag cgcggtcaga
      421 acggcctggt gcacctgctc ggcggtgcca tccccgtcga tccgggcgaa ccgcgccggc
      481 tcgtcttcgg caaagcgcga aaatgccttg gctacgcttg cgtgataggc ggcatcgcgg
      541 ccaccgatcc gatcgggatt gtccccatcg cgcactgcgg tccgagccat cgccacggct
      601 ggcgaaacct cgatcagcaa ggtcagatcc ggcagcagcc caccgctgcc gatccggtgc
      661 agttcgagca catcggtgtc ggaaaggccc ccggcaccgc cctggtaagc ccggctcgaa
      721 tcgacaaaac ggtcgcagat cacccatttt ccagcttcga gcgctggctg gataaggcgt
      781 tcaacgtggt cggatcgcgc ggcggcaaac agcagagctt cggcccgcgg gtgccagccc
      841 tccccatcgg ttcccagcaa aagggcgcgg atcgcctcgg cgccaggtgt tccgccgggt
      901 tctcgcgtga ctagcgcggt gatcccgcgc gcatcgagcg cgcgttccag caggcgcgcc
      961 tgggtgctct tgccagcccc ttccccgcct tcgaatgcaa tgaatttccc ccggctcatt
     1021 ccaatagtcc tagcaggcca ttgcgcagtc tgtcgattgg cccggccctt gcgacatccc
     1081 gcgtggcgac aagcggcagg aaatgatccg gttgcccttg catgtgcact tgcagccccg
     1141 ccacaaccgt tcccttgcct atcggcgcgc gcaatgggcc cgagtagatg atccggccgg
     1201 cgacgggcgg tttggttcct ttcggaaccg tcagcgcgaa cgggcgggca accgacagtg
     1261 gaacctggcg cacggcacca tcctgaaccc tggccgcgcc gactatccag gacctgtcca
     1321 gaaagggcct gctatcccat ctggcatagc cccactctgc cagctccgtc gctgccagcg
     1381 cacgtgccga ttcgctcggc gcacggccga tcaccaggat cagccgccgt ccttcgcgct
     1441 ggaccgcgcc tagaaaggta tagccggcct ggctggtgaa accggtcttt atgccatcgg
     1501 cgccgggcag aatttcggcc agggggtcgt ggctggtcag caccgcgccg cgccaggcaa
     1561 agctcttgcg gccgatataa cgccggtaaa gttcgggatg ctcgacgatc agcgcctggg
     1621 cagggcgcac catgtcgcgg gcggtaacat aggtcttgcc gccatctggc caaccgttgc
     1681 tggtggcgaa atggcttgcg ttcatcccca gttcttgcgc gcgccggttc atcgcccggg
     1741 tccaggcggc ttcgctgccc agcgccgctt cggccagcac caccgccgca tcgttggccg
     1801 aaacggtatt gagcccgcgc agcaggtcgt gcaccgaaac ctgttcgccc gcccgcaggt
     1861 tgaggctggt ccccttccct gcccagcgcg ccgcggtttg cggacgaacg gtgaccatat
     1921 cgctctctcg cagcttgcca gccttgatca ggtcaaaggc caccagcgcg ctcatcgctt
     1981 tggtcatcga ggccgggatc atcctggctt cggcgttacg ggcatagaga gtctggcccg
     2041 ccgaaagatc gaccagcagg ccaataggcg ttctggtggt tggtggcggc acgatctgcg
     2101 caggggttga acttgtgttc gccgccgccg gcccagcgag gataagagcc agaaccagga
     2161 ctacccttgc actgttcaac gcttctcccc cacatcgggc atcacccgag cgagcgagat
     2221 tgccagacgc tagtttgatc gcaggattcg tgcgtcgcta tagcctgcgg cacgggccct
     2281 ggccaaggcc gccttggcat cggcttcgct ggcgaaaggc cccatcctga ccgaccagag
     2341 cttgcccgac tgattggccg atccgccact tttccgggcg gcggcatcgg cattggcctt
     2401 gctagaatag gccccgacct ggaccaagaa acggccagcc gattggggct gcttggcacc
     2461 tggcttgggt tcggcgggtg cctggacagc cggctttggc gcttccgctg gcttggcttc
     2521 cggtttcttg acctcgggct ttttgggagc tggctttttc ggttccggct taggcttggc
     2581 cggtgctgca ggcttggcct gcgtcggctg atccaccttt ggcgtggttg ccggttccgg
     2641 atcggcctgc accggggcgc gaaccacgcc ttcctgcgca tcgagcttgc gctgcagcac
     2701 aatcagaagc gatttgggcg tatccatgcg cgccggggcg gattgcccgg cacgcagcaa
     2761 agcccgctca ggctcaaccg ggttgacccg gcgtacccgc acggccgcct ggacagtacc
     2821 gaccatgccg agctgcgctg cggcacctgg tgaaagttcg atcagctgcg taccgttcat
     2881 cggcccgcgc cgttcaaccc gcaccaggat ggtccgcccg ctgtcaagcg cggttacctc
     2941 gacatagctg ggcagcggca gggtgtggtg cgcaccgctg accgcgctgc ccccctccgg
     3001 gctcacgctt gcccgcccca ccgcatcata attcatcgtg tccaccggcg tgaagttggt
     3061 tccgttcacg acatagggcg caccgaccac gatcggataa tccgccgcag ggccattctt
     3121 tggcacgact ggcgcgccag ccaggcgcgt atcaccaccg ccacccatgc aggcgttcag
     3181 caagaccagc gctgccaggg gcaaagcgcg actaacgggc aatttcatct gcaagcaacc
     3241 ccacggacag tgcgtagtag ttcgagcagt tataatggag gatgacccga taattcccgg
     3301 ttaacaggaa tgaaccattc ccgggcccgt cgggttcgaa cagcgtcgcc agggtcgtat
     3361 cggcgatggg agcctgcgga attatgccga gcgagcgcca ttcggccacg gttttccact
     3421 gcgaatggcg attgtgcacg cgcggacaag tgggagcctc cagctctgcc ttgaacccgc
     3481 tccgatcgaa ctgcgccgga acctgagccc gaacccccca gggctccccg ggccgccagc
     3541 cggcgtcgcg aaaatagttg gcgatcgata ccagcgtgtc gcgctggctg gcccagatat
     3601 cggccaggcc atcgccatca ccatcccggg ccagacgcaa atagacactg ggcaggaatt
     3661 gcggattgcc gaatgctccg gcccacgacc ccaccagctt ctggcgcggc acgccgcgat
     3721 ccaccatttt caggactgcc accaattcgc cttcgaacag ttcgcgtcgc cgtccttcgt
     3781 aagccagcgt tgccaggctg cgcggcaggt cgaaatcccc ctgcacccgg ccatagaacg
     3841 attccttgcc ccagatcgcg atcagcatct tggccggaac gccatattcc gcctcgatcg
     3901 aactggtgat gtcgcgtgtg tcggcaagcc tggcccgccc actctcgatc cgggcggcgc
     3961 tgatggtccg gttgtaatat ggccagaacg ccggaggtga attgggggtt gatccgggtt
     4021 gcgagcggtc aagcgcgacc acccgggggt tataggtcag gcccgagagc accgactgga
     4081 tcgtttcctc gctcacccct tcgctccgcg cccgcgccgc aatcagttgc agataaccgg
     4141 caaaaccggc gtcaactgaa ggcactgcgg cgggcgcttc actgcctgcg gggggcgcag
     4201 cgatggttgc cgattgcgcc agatcggtgg gcggagcttc ctgggcaatg cttgaggaaa
     4261 gcagcgaagt cgcaagcagc aatggagcga tcagctttgc ggcgtgcggc aggctcatgg
     4321 gcaacctctt gcacagcaat tgcgattcgc caaacccatc gggcgtgaca ttgcgccgca
     4381 atgacgctag cgggcgtcca accggacagg tggccgagtg gtttaaggca gcggtcttga
     4441 aaaccgccgt gggtgcaagc tcaccgtggg ttcgaatccc accctgtccg cca
//
LOCUS       DASUDV010000481         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2026691,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000481 DASUDV010000000
VERSION     DASUDV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026691"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFV30_10400"
     CDS             complement(<1..822)
                     /locus_tag="VFV30_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX4848542.1"
                     /translation="MSQTQRRGLGFWALLALVIGNMIGSGIYLLPATLAPLGWNQMIG
                     WLITIVGALSLAVVFAGLSARIPRAGGPYAYADAAFGSLAGFLAAWAYWVMTWVGNGA
                     IAVAVISNLSLIFPVIGQTPFLPALLAIACIWLLTLVNISGVRSAGRVQEVTTLLKVI
                     PLVALILLAIWFALSGAPRAPDPGIPLTSDNIALAVGLTFWAFLGLESGTVPADKVEN
                     PERNVPRATIIGIASAGLIYFGISLSFAFYMPVDEAAASPAPVADFLGKTFGSGIA"
     gene            900..2525
                     /locus_tag="VFV30_10405"
     CDS             900..2525
                     /locus_tag="VFV30_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX4848543.1"
                     /translation="MSNAPQPAPAGYHPRLIKAALALRQNALHEAEPLLKQHLREDPF
                     DVYAIRMLAELAGRIGRMRDAENLLRRALELAPGFTAARSNLALLLHRANRPEEALGE
                     LEQVRSEDPDDPGHATLHAAVAGRIGAFDEAIALYETILARPNAPAPIWLSYGHTLKT
                     VGRSEDGVAAYRQALALRPSFGEAWWSIANLKNVKLDEHDRAAMLAALEQGGISDEDR
                     FHLHFALGKSLDDARQGEAAFDHYLEANRLRRASLPYDAAETTRAVDKIIARCDAAFF
                     AARAGQGCQASDPVFILGMPRAGSTLIEQILSCHSQVEGTMELPDIPAMVGKLGRHAA
                     GWVEALAELDDEGLRGLGESYLQTTRIQRREGKPLFIDKLPNNWLFTALIHLILPNAR
                     IIDARRHPLDCGYSNFRQHYARGQAFAYDLADIGHYYADYVRLMDHFDKVLPGRVTRV
                     IHEALLDDPEGEIRSLIERLGLPFEEQCLDFANSGRAVRTASSEQVRKPINRSGEGQW
                     RPVEDRLGPLIGALGPVLGAYPAVPEEWRQGHA"
     gene            2633..>2941
                     /locus_tag="VFV30_10410"
     CDS             2633..>2941
                     /locus_tag="VFV30_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEX4848544.1"
                     /translation="MKKSASLRQRSFVLLATTAAIMPASAFAQDAAAADDTTEIVVTA
                     QRREEKLRDVPISITALGEGKLEQANVQSFEDYAKLLPSVSFQSFGPSQSQIFFRGVS
                     S"
BASE COUNT          534 a          994 c          932 g          481 t
ORIGIN      
        1 ggcgatgccc gaaccgaagg tcttgcccag gaaatcggcc accggtgcgg gtgaggccgc
       61 ggcctcgtcc accggcatgt agaaggcgaa ggacagcgag ataccaaaat agatcagccc
      121 ggcactggcg atcccgatga tcgttgcgcg cggcacgttg cgttcggggt tttcgacctt
      181 gtcggccggg actgtcccgg attcgagccc caggaaggcc cagaaggtaa gccccacggc
      241 cagcgcgata ttgtcgctgg tcaggggaat gccgggatcg ggcgcgcgcg gcgcgccgct
      301 cagcgcgaac cagattgcaa gcaggatcag cgcgacgagc gggatgacct tgagcagcgt
      361 ggtcacttcc tggacccggc cggccgatcg cacccccgaa atgttcacca gggtcagcag
      421 ccagatgcag gctatggcca gcagcgcggg aagaaatggg gtctggccga tcaccggaaa
      481 gatcaggctg aggttgctga tcaccgccac tgcgatcgcc ccgttgccga cccaggtcat
      541 tacccaatag gcccaggcgg cgaggaaccc ggccagcgat ccgaaggcgg catcggcata
      601 ggcatagggt ccgccggcgc gcgggatccg ggcggaaagg ccggcaaaga caaccgccag
      661 gctcagcgcg ccgacgatcg tgatcagcca gccgatcatc tggttccagc ccagcggggc
      721 cagcgtggcg ggcagcagat agatgcccga accgatcatg ttgccgataa ccagcgccag
      781 cagcgcccag aatccaagcc cgcgtcgctg tgtctggctc atcctcgaaa atcccctctc
      841 cccaagtgtc tgcaacctaa cgcttgatcc ggcggcgtca ccatgtttct tgggtgatca
      901 tgtcgaatgc cccgcaaccc gcgcctgctg gctatcaccc gcgcctgatc aaggccgcgc
      961 tggcgctgcg ccagaacgcg cttcacgagg ccgaaccgct gctcaagcag cacctgcgcg
     1021 aagacccttt cgatgtctac gcgatccgca tgctggccga actggcgggc cggatcggcc
     1081 ggatgcgcga tgccgaaaac ctgctgcggc gcgcacttga actggcgccc ggcttcaccg
     1141 cggcgcgttc caacctggcg cttctgctgc accgtgcgaa ccgtcccgaa gaggcgctgg
     1201 gcgaactcga acaggtccgc tccgaagatc ccgacgatcc cggccacgcc acgctccacg
     1261 ccgcggtggc cggacggatc ggtgccttcg acgaggcgat cgcactttac gaaacgatcc
     1321 ttgcccggcc caatgccccg gcgccgatct ggctgtccta tggccatacc ctgaaaaccg
     1381 tgggccggtc cgaggacggc gttgccgctt atcgccaggc gctggccctg cgccccagct
     1441 tcggcgaggc ctggtggtcg atcgccaacc tcaagaacgt aaagctggac gagcacgatc
     1501 gcgccgcgat gctcgccgcg ctcgagcagg gcgggatcag cgacgaggac cgctttcacc
     1561 tgcacttcgc gctgggcaag tcgctcgacg atgcccgcca gggcgaggca gccttcgatc
     1621 actatctcga ggcgaaccgc ctgcgccgcg ccagcctgcc ctatgacgcc gccgagacca
     1681 cccgcgcggt cgacaagatc atcgcccgat gcgatgcggc cttcttcgcc gcgcgggcag
     1741 gacaaggctg ccaggcgagc gatccggtgt tcatcctggg catgccgcgc gccggatcga
     1801 cgctgatcga acagatcctc tcgtgccatt cgcaggtcga agggacaatg gaactgcccg
     1861 atattccggc gatggtcggc aagctgggcc gccatgccgc cggctgggtc gaggcgctgg
     1921 ccgaactgga cgatgaaggt ctgcgcgggc ttggcgaaag ctatcttcag accacccgaa
     1981 tccagcgccg cgagggcaag ccgctgttca ttgacaagct gcccaacaac tggctgttca
     2041 ccgccctgat ccacctgatc ctgcccaatg cccggatcat cgacgcgcgg cgccatccgc
     2101 tcgattgcgg ctattcgaac ttccgccagc actatgcccg cggccaggcc tttgcctatg
     2161 atctggccga tatcggtcac tactatgccg actatgtccg gctgatggac catttcgaca
     2221 aggtcttgcc cggccgggtc acccgggtga tccacgaagc gctgctcgac gatcccgaag
     2281 gcgagatccg tagcctgatc gaacggctgg gcctgccgtt cgaggagcaa tgcctcgact
     2341 tcgcgaactc gggccgggcg gtgcgcaccg ccagcagcga gcaggtcagg aagccgatca
     2401 atcgcagcgg cgaaggtcaa tggcgcccgg ttgaagaccg gctggggccg cttatcggcg
     2461 cgcttggccc ggtgctgggc gcctatcccg ctgttcctga agagtggcgc caaggccacg
     2521 cctgaccggt aaacggtcgg ggcggggcgg ggtttggtcg atttgcggtt gtttcaccgc
     2581 tgtcctgcga caataggccc gttcattcga tttaggacag tttggaggtc ccatgaagaa
     2641 gtctgcttcc ctgcgtcagc gctcgtttgt gctgctggca accactgcgg caatcatgcc
     2701 ggccagcgcc tttgcccagg acgccgccgc cgcggacgac accaccgaga tcgtcgtgac
     2761 cgcccagcgc cgcgaggaaa aactgcgcga cgttccgatc agcatcaccg cgcttggcga
     2821 aggcaagctg gaacaggcca acgtccagtc gttcgaagac tatgccaagc tgcttcccag
     2881 cgtctcgttc cagtcgttcg gccccagcca gtcgcagatc ttcttccgcg gcgtttcctc
     2941 g
//
LOCUS       DASUDV010000482         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_436055,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000482 DASUDV010000000
VERSION     DASUDV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436055"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..459)
                     /locus_tag="VFV30_10415"
     CDS             complement(19..459)
                     /locus_tag="VFV30_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4848545.1"
                     /translation="MGMGPAPSRRTRRGRTPMADINVTPLVDVMLVLLIIFMVTAPLL
                     SAGVPVNLPDANAKPLNDNPSEISLSIATDGVIYLGEEALAPGSLAQKLAEVPPGPDG
                     KPPQVVLRADRSLDYGRVMAVMGELNRAGFTAISLVTTGSVPSP"
     gene            complement(459..1163)
                     /gene="tolQ"
                     /locus_tag="VFV30_10420"
     CDS             complement(459..1163)
                     /gene="tolQ"
                     /locus_tag="VFV30_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX4848546.1"
                     /translation="MTVLALLNAAAAAPKHLDPLALFLQADIVVQAVMAGLVLASVWT
                     WTIIAGFSLRHARLRKDCDSYERDFWKAPDLDAFQAERGKADIPSARVAAAGIGEWRR
                     STGGKTLDRDGTRQRLAQAMDSAIAQEADKLAERLNFLATVGSVAPFVGLFGTVWGIM
                     NSFFQIGQQQNSSLAVVAPGISEALFATAIGLFAAIPAVIAYNRFSHRVNQLEARLQR
                     FADRFHASLSRELDAQ"
     gene            complement(1160..1618)
                     /locus_tag="VFV30_10425"
     CDS             complement(1160..1618)
                     /locus_tag="VFV30_10425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX4848547.1"
                     /translation="MTSQPSPPSGLIEGTLHRFAVRAYFEDTDLSGVVYHANYLRWFE
                     RARSDFVRLLGIDQRAVNEAGTGAFAVADLSIRYAAPARLDDAVLIETRCTELGAASC
                     RMHQKAFREDGLLLTEATFRVGFVSPDGRPRRMPDAWRAAFATIMPEGPR"
     gene            complement(1711..>2040)
                     /locus_tag="VFV30_10430"
     CDS             complement(1711..>2040)
                     /locus_tag="VFV30_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX4848548.1"
                     /translation="KILRGEIPNRTVYEDEWALAFHDINPQAPLHVLVIPKGPYVSWD
                     DFSARAGAEEIAGFIRAVGHVAREAGLVEPGYRLLANIGGHGHQEVPHLHVHLFGGRP
                     LGPMLAR"
BASE COUNT          334 a          673 c          731 g          302 t
ORIGIN      
        1 ctgggccatg gcgcagggct atgggcttgg aactgaaccg gtggtgacca gcgatatcgc
       61 ggtgaagcca gcgcggttga gctcgcccat caccgccatg acccggccat agtccaggct
      121 gcggtcggcc cgcagcacca cctgcggcgg tttgccgtcg ggcccgggcg gcacttcggc
      181 cagtttctgc gccagcgacc cgggcgccag cgcctcttcg cccagataga tcacgccgtc
      241 ggtggcgatc gacaggctga tctcgctggg gttgtcgttc agcggcttgg cattggcatc
      301 gggcagattg accggcaccc cggcgctgag cagcggggcg gtgaccatga agatgatcag
      361 cagcaccagc atgacgtcga ccagcggcgt cacgttgatg tccgccatcg gggtgcggcc
      421 ccggcgggtg cggcggctgg gagcgggacc cattcccatt actgcgcgtc cagttcccgg
      481 ctgaggctgg cgtggaagcg gtcggcaaag cgctgcagcc gcgcttccag ctggttcacc
      541 cggtgcgaaa agcggttgta ggcgatgacc gccgggatcg ccgcgaacag gccgatcgca
      601 gtggcgaaca gggcctcgga aatccctggc gcgaccacgg cgagcgagga gttctgctgc
      661 tgcccgatct ggaagaagct gttcatgatc ccccagacgg tcccgaacag cccgacgaag
      721 ggcgcgaccg agccgaccgt ggcgaggaag ttgagccgtt cggccagctt gtccgcttcc
      781 tgcgcgatcg cgctgtccat tgcctgggcg agccgctggc gggtgccgtc gcggtccagc
      841 gtcttgccgc cggtggagcg gcgccattcg ccgatcccgg ccgcggcaac ccgggccgag
      901 gggatgtcgg ccttgccgcg ctcggcctgg aaagcgtcca gatcgggcgc cttccagaaa
      961 tcgcgctcat agctgtcgca atccttgcgc agccgggcgt ggcgcaggct gaagccggcg
     1021 atgatcgtcc aggtccagac cgaggccagc accagcccgg ccatcaccgc ctggacgacg
     1081 atgtcggcct gcaggaacag cgcgagcgga tcgaggtgct ttggcgcggc ggccgcggca
     1141 ttcagcaggg caagaacggt catcggggtc cttcgggcat gatcgtggca aaggccgcgc
     1201 gccaggcatc gggcatccgg cgcgggcggc catcggggga aacgaaaccg acgcgaaagg
     1261 tcgcctcggt cagcagcagg ccgtcctcgc gaaaggcctt ctggtgcatc cggcacgagg
     1321 ccgcgccgag ctcggtgcag cgggtctcga tcagcacggc atcgtccagc cgggccggcg
     1381 cggcatagcg gatcgacagg tcggcaacgg caaaggcgcc ggtgcccgct tcgttcaccg
     1441 cgcgctgatc gatccccagc aggcggacga aatcggaccg ggcgcgttcg aaccagcgca
     1501 ggtaattggc gtgatagacc acccccgaca ggtcggtgtc ctcgaaatag gcgcgcacgg
     1561 caaagcggtg cagcgtgcct tcaatcaggc cggagggggg cgaaggctgt gacgtcatgc
     1621 cggcggaccc ttagcaagtc cgcgagtcgc agggcaaggg gccttgccgg ttgtgtgcct
     1681 caactgcgcc gaagcgagcc gcttggcgct tcagcgcgcc agcatcggcc cgagcgggcg
     1741 gccgccgaac aggtggacgt ggaggtgcgg cacctcctgg tggccgtgcc cgccgatatt
     1801 ggcgagcagc cggtaccccg gctcgaccag cccggcctcg cgcgcgacat ggccgaccgc
     1861 gcggatgaaa ccggcaatct cctcggcccc ggcgcgggcc gaaaagtcgt cccagctcac
     1921 ataagggccc ttggggatca ccagcacgtg gagcggggcc tgcgggttga tgtcgtgaaa
     1981 ggccagcgcc cattcgtcct cgtaaaccgt ccggtttgga atctccccgc gcaggatctt
//
LOCUS       DASUDV010000483         7983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_966278,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000483 DASUDV010000000
VERSION     DASUDV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966278"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFV30_10435"
     CDS             <1..389
                     /locus_tag="VFV30_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848549.1"
                     /translation="VPRAVSLTEFISAFYTSGAFRPERWLLGALLGKRADDSDVARLA
                     AGQSERFSAWSVEARREDQILLCDYQGRTRSWLMVSPTEGDTRLHFGSAVVPAKGRAE
                     RGVFRALLWFHRWYSVHLLSGAVSRL"
     gene            complement(399..1493)
                     /locus_tag="VFV30_10440"
     CDS             complement(399..1493)
                     /locus_tag="VFV30_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848550.1"
                     /translation="MRRQIALVAALAAPFLAAPASSQVVGEWKFRLEEAQSVTLAVTR
                     DPSNPGILIGEGTIDGRRLVFVGEFRNLKWVGTWYWYGPGSRRLPGLRTCAAPVAPRP
                     GTPGYGTRTTHTGGFEFTFNAAEDRITGSWKSACSGADGRAESGLPVAFLARRMNTYA
                     AAPPTTTYRPAAASTGSSGASPAPLDPRLAGEDRVDDSPCTARFGLRLSATGRSAGPA
                     TVFSARYRLRPCMTSAGKWVQVDMLSPEGKRPIRLVLQGLSVRGIVPAGERQELLASP
                     SGRTLQQGLRFVGEPRAGEVISRIGMSGGICSAPVWLAWLDFSDGTRSREPIGLLVSA
                     CGAQAHPEMLPPRPPEPEDIRPAGEATPRP"
     gene            complement(1587..2228)
                     /locus_tag="VFV30_10445"
     CDS             complement(1587..2228)
                     /locus_tag="VFV30_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX4848551.1"
                     /translation="MSLLLGACSVLPQSRAPQAKTGVSRSTQQITPRPEVRACLADLG
                     ARKAAFSPLPDQYYGAGCSTIGTVRLSALRSDDAKLALTNLGPVTCPLADAFAGWARY
                     GADRAAQQILGSPIIRIETMGSYNCRNVAGSSRRSAHATANAIDVSGFVLADGRRITV
                     AGNWNDKSPQVRRFFTAIHASACKRFGTVLGPAYNAAHRDHLHLELSGSAFCR"
     gene            2428..3555
                     /locus_tag="VFV30_10450"
     CDS             2428..3555
                     /locus_tag="VFV30_10450"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX4848552.1"
                     /translation="MTDTMPARKPARPFFSSGPCAKPPGYSPAKLNTESLGRSHRAKI
                     GKTRLAYCIDLMRDVLQLPDTHRIGIVPGSDTGAFEMAMWTMLGARGVTCLAWESFGE
                     GWVTDAVKQLKLEPKVHRADYGQLPDLSKVDFADDVLFTWNGTTSGVRVPDGEWIPDD
                     REGLTFADATSAVFAYDIPWDKIDVATFSWQKVLGGEGGHGVLILGPRAVQRLEEYTP
                     AWPLPKVFRLTSKGKLSEGVFKGETINTPSMLAVEDAIFALEWAKGLGGLDGLMARSN
                     ANAHALDKIVAERDWLSHLAADSGIRSKTSVCLSVEGADADFIKAMTGALEKAGAAYD
                     IAGYRDAPPGLRIWCGATVETADIEALGPWLDWAYAVTKSA"
     gene            3684..5267
                     /gene="serA"
                     /locus_tag="VFV30_10455"
     CDS             3684..5267
                     /gene="serA"
                     /locus_tag="VFV30_10455"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX4848553.1"
                     /translation="MTKPRVLISDKMDPNAARIFEERGCDVDVITGETPEQLIARIGD
                     YDGLAIRSSTKVTKAVLDAAKNLKVVGRAGIGVDNVDIPAASAQGVVVMNTPFGNSIT
                     TAEHAIALMFALARQIPEANAQTQAGKWPKNDFMGIEVTGKTLGLIGAGNIGSIVASR
                     ALGLKMKVVAYDPFLTPERAVEMGVEKADLDTVLAKADFITLHTPLTDQTRNILSREN
                     LAKTKKGVRIINCARGGLIDEEALKEALDSGQVAGAALDVFQTEPAKESPLFGTPNFI
                     CTPHLGASTNEAQVNVALQVAEQMADYLVSGGVTNALNMPSLSAEEAPKLKPYMALAE
                     KLGSLVGQLTTGSIPRVSIHVEGAAAELNIKPIVAAVLCGFLRVQSATVNMVNAPFLA
                     KERGIEVREVRTEKEGDYHTLIRVSVKTDAGERSVAGTLFSNSEPRLVELFGIKVEAE
                     LAGYMMYIVNEDAPGFIGRIGTLLGENSINIGTFNLGRREAGGEAILLLSVDSPVPAT
                     VMDQAKVLPGVKRVMALAF"
     gene            5433..6557
                     /locus_tag="VFV30_10460"
     CDS             5433..6557
                     /locus_tag="VFV30_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4848554.1"
                     /translation="MDSDRDLLPEGLEDRLPAAAAAAARVTRAVIDVLDGHGYDRVQP
                     PTIEFEKSMASRMVGVQPRRMFRFVDPASLRTLALRSDITVQVGRIAATALAARARPL
                     RLCYAGQVVTIKGDGLDPTRERLQLGAELIGSDSVAAAGEIVAAAIEALHAAGATGLS
                     VDFTLPDLVDTLAGVDGIGALPLDPDKIEAVRRELDAKDAGGLVAAGGEAYLPLLTAI
                     GPFAAAIERLAAIDAGGALSSRIAALREVAARVGTKARLTLDPSERHGFEYQSWFGFT
                     IYAEGVPGALGRGGSYRILGLDEPAIGFSLYPDALIDALAGSEAARDTLFLPLGHDPV
                     HAERLRAIGWRTVAAICEACDPAALGCTHVLEGGEAVKLG"
     gene            complement(6654..7574)
                     /locus_tag="VFV30_10465"
     CDS             complement(6654..7574)
                     /locus_tag="VFV30_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848555.1"
                     /translation="MKRALILTLAASAMLAGTLAEARPGSRLRDRLAERGSTDSAAPQ
                     APAKQTLSYGTDPLQALDFYPAAGGNRRAPLVIFVHGGGWKRGSKDNASGPWKAPHYT
                     GLGYAYASINYRLVPDATVEQQGADVAAAVRYLIDNAARLGFDSTRIVLMGHSAGAHL
                     VALVGTDESYLKGAGLSFAAIDGVIPIDGAAYDVPRQMSEGGKFMSDTYLQAFGSDPA
                     RQRALSPTLHAQAPNAPAFLIIHVQRKDGVAQSVALEAALKKGRTRAERQEFEGRGLK
                     GHAEINRQLGNPDYAATPVVDAWLKKVFGS"
     gene            7808..>7983
                     /locus_tag="VFV30_10470"
     CDS             7808..>7983
                     /locus_tag="VFV30_10470"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX4848556.1"
                     /translation="MANVTVIGAQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLV
                     VGENVYKLSLLPSG"
BASE COUNT         1272 a         2673 c         2782 g         1256 t
ORIGIN      
        1 acgtgccgcg cgcggtctcc ctgaccgagt tcatcagcgc cttctacacc agcggcgcgt
       61 tccggccgga gcgctggctg ctgggcgcgc tgctgggcaa gcgcgcggat gacagcgacg
      121 tggcgcggct ggccgcgggt cagagcgagc gcttttcggc ctggtcggtc gaggcgcgcc
      181 gtgaggacca gatcctgctg tgcgactacc agggccggac ccgctcgtgg ttgatggtct
      241 cgcccactga aggcgacacc cggctgcatt tcggttcggc cgtggtcccg gccaagggcc
      301 gggccgaacg gggcgtgttc cgcgcactgc tctggttcca ccgctggtat tcggtgcatt
      361 tgctgagcgg cgcggtcagc cggctctagg gacctgacct aaggccttgg cgtcgcttcg
      421 ccggcggggc ggatgtcttc cggttcggga gggcgcggcg gcagcatctc cggatgggcc
      481 tgcgcaccgc aggccgatac gagcaggccg atcggttcgc ggctgcgcgt gccgtccgaa
      541 aagtccagcc aggccagcca caccggtgcc gagcagatgc ccccgctcat gccgatccgg
      601 ctgatgacct cgccggcccg cggctcgccg acaaaccgca agccttgctg cagggttcgg
      661 cccgacgggc tggccagcag ctcctgccgc tcaccggccg gcacaatgcc ccgcacgctc
      721 aggccctgca gaaccagccg gatcggacgc ttgccttccg ggctcagcat atcgacctga
      781 acccatttgc cggcactggt catgcacggg cgcaggcggt agcgggccga gaacacggtt
      841 gccggaccgg cagaccggcc ggttgccgac aggcgcaagc caaagcgggc ggtgcagggg
      901 ctgtcgtcta cccgatcttc gcccgcaagc cgcgggtcaa gcggtgcggg gctggcaccg
      961 gacgagccgg tggacgcggc agccgggcgg taggttgtgg tcggcggagc cgccgcatag
     1021 gtgttcatgc ggcgcgccag gaaagcgact ggcaaaccgc tctccgcgcg cccgtcagcg
     1081 cccgagcagg ccgatttcca tgagccggtg atccggtctt ccgcggcgtt gaacgtgaat
     1141 tcaaaaccgc cggtgtgcgt cgtgcgggtg ccatagcccg gggttccggg gcgcggcgca
     1201 accggcgcgg cacaggtgcg cagacctggc aaacggcggc tgcccgggcc gtaccagtac
     1261 caggttccca cccatttgag gttccggaac tcgccgacaa acaccagccg gcgcccgtcg
     1321 atcgtgcctt cgccgatcag gattcccgga ttggacggat cgcgcgttac cgcaagggtc
     1381 acgctttggg cttcttcgag gcggaatttc cactcaccaa ccacctggct ggaagccggg
     1441 gcggcgagaa acggggcggc aagggccgcg accaacgcga tctgtctgcg catcattacc
     1501 tccgcagcga ctcgaagggt atccgctcgc tggcaggcta tcgtaaatcg gcctgcgcgc
     1561 aaaccagacg cggcggccga gtgcggtcag cggcagaagg cgctaccgct cagttccagg
     1621 tgcaggtgat cgcggtgggc ggcgttgtag gccgggccca gcaccgtgcc aaagcgcttg
     1681 caggcgctgg cgtggatcgc ggtgaagaag cggcggacct ggggcgactt gtcgttccag
     1741 ttgccggcca cggtgatccg ccggccatcg gccaggacaa agcccgacac gtcgatcgcg
     1801 ttggcggtgg cgtgggcgct gcggcggctc gatccggcga cgttgcggca gttgtagctg
     1861 cccatcgttt cgatccggat gatcggactg cccaggatct gctgcgccgc ccggtccgcg
     1921 ccatagcggg cccagccggc gaaggcatcg gccagcgggc aggtgaccgg gcccaggttg
     1981 gtcagcgcca gcttggcatc gtcgctccgc agggcggaga ggcggacagt gccgatggtc
     2041 gagcagcccg cgccgtaata ctggtcgggc aggggcgaga aggcggcctt gcgggcgccg
     2101 agatcggcca ggcaggcgcg cacttcgggc cgcggggtga tctgctgggt ggagcggctg
     2161 acaccggtct tggcctgcgg cgcgcggctt tgcggcagga cgctgcacgc gcccagcaag
     2221 aggctcaccg acaagaggat cgcagccttg cgcatggccg caagagtgcc cggaaaatgg
     2281 ttaacaagcc gcaaccggcc agggttaacg gcccgctaac cggtgaaccg tgcccggttt
     2341 cgcttgactt gttgcagcgc accattacca gcggctccgg gccacgcggt cccaacgccg
     2401 cgcgtgctct ctgcaaggag agactacatg actgacacga tgccggcgcg caaacccgcg
     2461 cgcccgttct tttcttccgg tccctgcgcc aagccgccgg gctattcccc cgccaaactg
     2521 aacaccgaat cgctgggccg ctcgcaccgc gcgaagatcg gcaagacccg tcttgcctat
     2581 tgcatcgacc tgatgcgcga cgtgctgcag ctgcccgata cgcaccggat cggcattgtt
     2641 ccgggcagcg acaccggcgc ctttgaaatg gcgatgtgga ccatgctggg cgcccgcggc
     2701 gtgacctgcc tggcctggga aagcttcggg gaaggttggg tcaccgatgc ggtcaagcag
     2761 ctgaagctgg aacccaaggt gcaccgcgcc gattacggcc agctgccgga cctttccaag
     2821 gtcgatttcg ccgacgacgt gctgttcacc tggaacggca cgaccagcgg cgtgcgcgtg
     2881 ccggacggcg aatggatccc ggatgaccgc gagggcctga cctttgccga cgccaccagc
     2941 gcggtcttcg cttacgacat tccctgggac aagatcgatg tcgccacgtt ctcctggcag
     3001 aaggtgctgg gcggcgaggg cggccacggc gtcctgatcc tgggcccgcg cgcggtgcag
     3061 cggctggagg aatacacccc ggcctggccg ctccccaagg tgttccgcct gacctccaag
     3121 ggcaagctct ccgagggcgt gttcaagggc gagacgatca acaccccgtc catgctggcg
     3181 gtcgaggacg cgatcttcgc gctcgaatgg gccaagggcc tgggcgggct ggacgggctg
     3241 atggcccgca gcaacgccaa cgcccacgcg ctcgacaaga tcgtggccga gcgggactgg
     3301 ctgagccacc tcgcggctga cagcgggatc cgctcgaaga cctcggtctg cctttcggtg
     3361 gagggcgcgg acgcggactt catcaaggcg atgaccggcg cgctcgaaaa ggccggtgcg
     3421 gcctatgaca tcgccggcta ccgcgatgcc cctccgggtc tgcggatctg gtgcggcgcc
     3481 acggtcgaaa ccgccgatat cgaggcgctc ggcccctggc tcgactgggc ctacgccgtc
     3541 acgaaatccg cctgaatttc cgctcgtcct gagcttgtcg aaggactgtc cttttcttgc
     3601 ggcacagcgc cagcagtgaa ggacagggct tcgacaggct cagcccggac ggctctgttt
     3661 ttgctccatg tggagaattc caaatgacca agccccgcgt actgatttcc gacaagatgg
     3721 accccaacgc cgcccgcatt ttcgaagagc gcggctgcga tgtggatgtc ataaccggtg
     3781 aaacccccga acagctgatc gcccggatcg gcgattatga tggccttgcg atccgctcct
     3841 cgaccaaggt gaccaaggcg gtgctcgatg ccgccaagaa cctcaaggtc gtcggccgcg
     3901 ccggcatcgg ggtcgacaat gtcgatatcc cggccgcctc ggcccagggc gtggtggtga
     3961 tgaacacccc gttcggcaac tcgatcacca ccgccgaaca cgccatcgcg ctgatgttcg
     4021 cgctggcccg ccagattccc gaagccaacg cccagaccca ggccggcaag tggcccaaga
     4081 acgacttcat gggcatcgag gtgaccggta agaccctggg cctgatcggc gcgggcaaca
     4141 tcggctcgat cgtcgccagc cgggcgctgg gcctgaagat gaaggtcgtg gcctacgatc
     4201 cgttcctcac cccggaacgc gcggtcgaaa tgggcgtgga gaaggccgat ctggatacgg
     4261 tcctcgccaa ggccgatttc atcacgctgc acaccccgct gaccgaccag acccgcaaca
     4321 tcctgagccg ggaaaacctg gccaagacca agaagggcgt gcggatcatc aactgcgcgc
     4381 gcggcgggct gatcgacgag gaggcgctga aagaggcgct ggattcgggc caggtcgccg
     4441 gggccgcgct cgacgtgttc cagaccgaac cggccaagga atcgccgctg ttcggcacgc
     4501 cgaacttcat ctgcaccccg cacctggggg ccagcaccaa cgaagcccag gtcaacgttg
     4561 ccctgcaggt ggccgaacag atggccgact acctggtcag cggcggggtg accaacgcgc
     4621 tcaacatgcc gagcctctcg gccgaggaag cgccgaagct caagccctat atggcgctgg
     4681 ccgaaaagct gggcagcctg gtcggccagc tgaccaccgg atcgatcccg cgggtctcga
     4741 tccatgtcga aggcgcggcg gcagagctga acatcaagcc gatcgtcgcg gcggtgctgt
     4801 gcgggttcct gcgggtccag tcggccacgg tcaacatggt caacgccccg ttcctggcca
     4861 aggagcgcgg gatcgaggtg cgcgaagtgc ggaccgagaa ggaaggcgat taccacaccc
     4921 tgatccgcgt ttcggtgaag accgacgcgg gcgaacgctc ggtcgccggc acgctgttct
     4981 ccaacagcga accgcgcctg gtcgaactgt tcgggatcaa ggtggaagcc gagcttgccg
     5041 gctacatgat gtacatcgtc aacgaggacg cccccggctt catcggccgg atcggcaccc
     5101 tgctgggcga aaattcgatc aacatcggca ccttcaacct cggccgccgc gaagcgggcg
     5161 gggaagcgat cctgctgctc agcgtcgaca gcccggttcc cgccacggtg atggaccagg
     5221 ccaaggtcct gcccggcgtg aagcgggtta tggcgctggc attttgaggc gcacaccgcg
     5281 cagcaggtgt tcccgaatgc ccctcctgct tgcgggaggg gttaggggag ggcctgttgc
     5341 tttgtcgcgg aaccgtgcct aacaggccct cccccgaccc ctcccgcaag cgggaggggg
     5401 gacaggggga agccactttc agggcttgaa ccatggattc tgatcgcgac ctgttgcccg
     5461 agggccttga agaccggctg cccgccgccg ccgccgctgc cgcgcgggtg acgcgggcgg
     5521 tgatcgatgt gctggacggc cacggctatg accgcgtcca gccgccgacc atcgagttcg
     5581 agaagtcgat ggccagccgc atggttgggg tccagccgcg ccggatgttc cggtttgtcg
     5641 atccggcaag cctgcgcact ctggcgctgc gcagcgacat cacggtccag gtcggccgga
     5701 tcgcggcgac cgccctggcc gcgcgggccc gcccgctgcg gctgtgctat gccgggcagg
     5761 tcgtgacgat caagggcgac gggctcgacc cgacgcgcga gcggctgcag ctgggcgcgg
     5821 agctgatcgg cagcgacagc gtggccgcgg cgggcgagat cgtggccgcc gccatcgagg
     5881 cgctgcacgc ggccggagcg accggcctgt cggtcgattt caccctgccg gacctggtcg
     5941 ataccctggc gggagtcgat ggcattgggg cgctcccgct cgatccggac aagatcgagg
     6001 cggtccggcg cgagctggac gccaaggatg ccggtgggct ggtggcggcc ggcggcgaag
     6061 cctatctgcc gctgctgacc gcgatcgggc cgttcgcggc ggcgatcgag cggctggccg
     6121 cgatcgatgc cggcggggcg ctgtcctcgc gcatcgccgc actgcgcgaa gtggcggcgc
     6181 gggttggcac caaggcgcgg ctgaccctcg acccctcgga acggcacggg ttcgaatacc
     6241 agtcgtggtt cggcttcacg atctatgccg aaggcgtgcc gggtgcgctg ggccggggcg
     6301 gatcctaccg gatcctcggc ctggacgagc cggcgatcgg cttttcgctc tatcccgatg
     6361 ccctgatcga tgccctggcc ggcagcgaag cggcgcgcga caccctgttc ctgccgctgg
     6421 gccacgatcc ggtccacgcc gagcgcctgc gggcgattgg ctggcgcacc gtggcggcga
     6481 tctgcgaggc ctgcgatccc gccgcgctgg gctgcaccca cgtgctggaa ggcggcgaag
     6541 cggtgaagct gggctgatcc ttcgccgggt ggtggagagg tacggaagag tgaccccgac
     6601 gcttccggcg atggtgtgat cccccgcccg tacccctctc tcgaacggga ggcctagctc
     6661 ccgaacactt tcttcagcca ggcatcgacc accggggtcg ccgcatagtc cggattgccg
     6721 agctggcggt tgatctcggc atgacctttc agcccgcgcc cctcgaactc ctgccgctcg
     6781 gcccgggtgc ggcccttctt cagcgcggcc tccagcgcga cggactgggc cacgccgtcc
     6841 ttgcgctgga catggatgat caggaacgcg ggcgcgttgg gcgcctgggc gtgaagcgtg
     6901 ggcgagagcg cccgctggcg tgccggatcg ctgccgaagg cctgcagata ggtgtccgac
     6961 atgaacttcc cgccctcgct catctggcgg ggcacgtcat aggccgcgcc gtcgatcggg
     7021 atgaccccgt cgatcgccgc aaagctgagg cctgcgccct tgaggtagct ttcgtcggtg
     7081 ccaaccagcg ccaccaggtg cgccccggcg ctgtggccca tcagcacgat ccgggtggaa
     7141 tcgaagccaa gccgggcggc gttgtcgatc agatagcgca ccgccgcggc cacatcggcg
     7201 ccctgctgct ccaccgtggc atccggcacc agccggtagt tgatcgaggc ataggcatag
     7261 ccaaggccgg tgtagtgcgg ggccttccac gggccgctgg cgttgtcctt gctgccgcgc
     7321 ttccacccgc cgccgtggac gaagatcacc agcggagcgc ggcggttgcc gccggcggcg
     7381 ggatagaaat cgagcgcctg gagcggatcg gttccatagg acagggtttg cttggccggg
     7441 gcctggggtg cggcgctgtc ggtgctgccg cgctcggcca gccggtcgcg caggcggctg
     7501 ccggggcggg cttcggccag ggtgccggcc agcatggcgc tggcggcgag ggtaaggatc
     7561 aaggcgcgtt tcatctggta actccctttg ccccgctatc gtgccccgca gattattgcc
     7621 gcaacctttc cgctccagcc gcaaattgtc gcagtttgtc gcacgccccg ccttgactcg
     7681 gcctcccccg gcaccctagg ggccgcaccg cacagcgggc cgatctgcgg tccaccggtc
     7741 catccctcgc gccgagtcct gtcgaagggc gcttgcggat ccccttggca ggggtaggac
     7801 tctatccatg gcaaacgtta ccgttatcgg cgcccagtgg ggcgatgagg gcaagggcaa
     7861 gatcgtcgac tggctggcca gccgggccga tgccgtggtg cggttccagg gcggccacaa
     7921 cgccggccac acgctggtgg tgggcgagaa cgtctacaag ctcagcctgc tgccctcggg
     7981 cat
//
LOCUS       DASUDV010000484         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2092966,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000484 DASUDV010000000
VERSION     DASUDV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092966"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1464)
                     /locus_tag="VFV30_10475"
     CDS             complement(88..1464)
                     /locus_tag="VFV30_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4848557.1"
                     /translation="MAAQSRLARLAAGLAALSVMVTPIVGHACTPSIVTEVFPKDFMK
                     AKCQQALANPWRFLGRIESGKIDGRELDNYVLAFDEGKYGCSENKSFVFKMLDRYYSV
                     GDRKLTQPWLLQRYAFNIPEDHDPAAREYVYNLLWLFNDTGTYLPRWWTAQAARAFVE
                     RPEHWPIALSRFGKMRERDDAVFASVADPQSRYFDRALALKLAGFPSKHQWQRKVAVA
                     SLYTDPRFGAPDLALAESLLPRSVLYSDQAATPWQQQARTLWGQIEQGYSQSADPALR
                     AKGQELRALMAPPVLDGWPAFDPPKDGRVWLSLADWPKAVPNPFAGKDVRRHLATPDD
                     YPTRARREELTGMVTVAARFGPDGKFVAVEVVRSSGVPALDTATTGLVTRRFRPKLSE
                     MTITGYPGREVMVPLLVVNWTISDDDPESGDAGAARFSDGMLTIVAPQAGIYAGYHDC
                     GSPPSYFL"
     gene            1614..2666
                     /locus_tag="VFV30_10480"
     CDS             1614..2666
                     /locus_tag="VFV30_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848558.1"
                     /translation="MYSEQDLQSAVSAGAITHEAAEALRAHAAGLRSAPVADEEHFRL
                     ITGFNDIFVTIAAVLLLVAAGGIGNAIAPGVAGVLIAAAAWGLAEFFTAKRRMALPSI
                     VLLLAFVGGVASIPINIIAEAETSWSAQTNALVGSGIGLLAALAAYVHWRRFMVPITV
                     AAGVAALAVTAIALVLSALSTSFGTQTDDVALLPAVFVAGLVVFAVAMRWDMSDPARE
                     TRRSDVAFWLHLLAAPMIAHPLFQWLGVSEGENIGAGSALGVLAVYVLMGMVALAVDR
                     RALLVSALAYVLFALTWLFREFGMVELNVAVTGLVIGSALLALSAFWTPIRMALIAQL
                     PGNLKARLPGGALLAA"
     gene            2791..>3131
                     /locus_tag="VFV30_10485"
     CDS             2791..>3131
                     /locus_tag="VFV30_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX4848559.1"
                     /translation="MAQLDYQARLASLRTRAVVAQVLLVLFMVVAGLVSLYTGILTWR
                     EFSTSYSTFLIVQPGMALFLLVAILPAAIGLSAWLWRAHANLHADGREALNYTPGWAV
                     GSLALPFVNLVV"
BASE COUNT          464 a         1066 c         1043 g          558 t
ORIGIN      
        1 cggcggcctg ggctgatcac agccgctggt ctgcgggccg taacgaaggg ggctggacgc
       61 gagtccggcc ccttttgctg tgcggtccta gaggaagtac gagggtggag acccgcagtc
      121 atggtagccc gcgtaaatgc cggcctgcgg ggcgacgatt gtcagcatcc cgtctgaaaa
      181 acgggccgcc cctgcatcgc cgctttccgg atcatcgtcc gagatggtcc agttcacgac
      241 cagcagcggc accatcacct cccgccccgg atagccggtg atcgtcatct cgctcagctt
      301 cgggcggaag cgccgggtca ccaggccggt ggtggcggtg tcgagcgccg gcaccccgct
      361 tgagcggacg acctccaccg ccacgaattt cccgtccggg ccaaaccgcg ccgccacggt
      421 gaccatgccg gtcagctcct cgcgccgcgc acgcgtcggg tagtcatcgg gcgtcgccag
      481 gtggcgccgg acgtccttgc cggcaaaggg attgggcacc gccttgggcc aatcggccag
      541 cgacagccag acccgcccgt ccttgggcgg atcgaaggcc ggccagccat ccagcaccgg
      601 cggcgccatc aacgcgcgca gctcttgccc cttggcgcgg agcgcgggat cggcgctttg
      661 cgaatagccc tgctcgatct ggccccagag cgtccgcgcc tgctgctgcc agggggtggc
      721 ggcctgatcg ctgtacagca ccgatcgcgg caagaggctc tccgccaggg ccagatcggg
      781 cgcgccgaag cggggatcgg tgtaaagcga agccaccgcg accttgcgct gccactgatg
      841 ttttgaggga aagcccgcaa gcttcagcgc cagtgcccgg tcaaagtacc ggctctgcgg
      901 atccgccacg ctggcaaaga ccgcatcgtc gcgttcgcgc atcttcccga accgggaaag
      961 cgcgatcggc caatgctccg gccgttcgac aaaggcgcgg gcagcctgcg cggtccacca
     1021 tctgggcagg taagtgccgg tatcgttgaa cagccaaagc aggttgtaga catactcccg
     1081 cgcggccggg tcgtggtctt ccgggatgtt gaaggcatag cgctgcagca gccagggctg
     1141 ggtcagcttg cgatcgccaa ccgaataata ccggtccagc atcttgaaca cgaaagactt
     1201 gttttcgctg cagccatact tgccttcgtc gaaggcgagg acatagttgt cgagttcccg
     1261 cccgtcgatc ttgcctgact cgatccggcc caggaaccgc cacggattgg cgagcgcctg
     1321 ctggcacttg gccttcatga aatccttcgg gaagacctcg gtaacgatag acggggtgca
     1381 ggcatggccg acgatcggcg tgaccatcac cgaaagggcg gcaaggcctg cggccagcct
     1441 ggcaaggcgc gattgagcgg ccatcgtggg gccgatgctc tgcctgtttc gcagcaaagt
     1501 caatgactcg cgctgcggca tccgccgcac gaccggggcg cactccacct accgtattga
     1561 cacactaaca cacttggccc atgctcccgc cattccccag gggaggataa ccgatgtatt
     1621 ccgaacaaga cctgcaatcg gcggtttccg ccggtgccat cacccacgaa gcggccgagg
     1681 ccttgcgcgc ccatgccgcc ggcctgcgca gcgcgccggt cgccgatgaa gagcatttcc
     1741 ggctgatcac cggcttcaac gacattttcg tcaccatcgc cgcggtgctg ctgctggtgg
     1801 cggccggcgg gatcggcaac gcgatcgcgc ccggcgtggc cggggtgctg attgccgccg
     1861 cggcctgggg cctggccgaa ttcttcacgg ccaagcgccg catggcgctg ccctcgatcg
     1921 tgctgctgct ggcctttgtc ggcggcgtgg catcgatccc gatcaacatc atcgccgaag
     1981 ccgagacgag ctggtccgcg caaaccaacg cgctggtcgg atcgggaatt ggcctgctgg
     2041 cggcgctggc cgcctatgtc cactggcggc gcttcatggt gccgatcacg gtcgccgccg
     2101 gggtggccgc gctggctgtc accgcgattg ccctggtcct ttcggcgctg tcgaccagct
     2161 tcggcaccca gaccgacgat gtcgcactgt tgccggcggt ctttgtcgcc gggctcgtgg
     2221 tctttgccgt ggcgatgcgc tgggacatga gcgacccggc ccgcgagacg cggcgcagcg
     2281 acgtggcctt ctggctgcac ctgctggccg cgccgatgat cgcccacccg ctgttccagt
     2341 ggttgggcgt gtccgaaggc gagaatatcg gcgccggatc ggcgctgggc gtgctggcgg
     2401 tctatgtcct gatgggcatg gttgccctgg cggtggaccg ccgcgcgctg ctggtttccg
     2461 cgctggccta tgtgctgttc gcgctgacct ggctgttccg cgaattcggc atggttgagc
     2521 tgaacgtcgc ggtgaccggc ctggtgatcg gttcggccct cctcgcgctc tcggccttct
     2581 ggaccccgat ccggatggcg ctgatcgcgc agctgcccgg aaacctgaag gcccgcctgc
     2641 cgggcggggc cctgctcgcg gcctgatcca gccactctca aacgcccatg ccggccccgg
     2701 tgctcgcaag cgcaccgggg ccggtttgca tcttgccgcg cgcgaatttc tccttatgcc
     2761 tcaagccttc ggcgcaagac gggggacggc atggcgcagt tggattacca ggcacggctg
     2821 gcttcgctgc gcacccgcgc ggtggtggcg caggtcctgc tggtgctgtt catggtcgtg
     2881 gcgggattgg tgtcgctcta caccgggatc ctcacctggc gggaattctc cacgtcgtac
     2941 tcgaccttcc tgatcgtcca gccggggatg gcgctgttcc tgctggtggc catcctgccc
     3001 gccgcgatcg gcctgtccgc ctggctgtgg cgcgcccacg ccaacctgca tgccgacggg
     3061 cgcgaggcgc tgaactacac gcccggctgg gccgtgggca gcctggccct ccccttcgtc
     3121 aacctggtcg t
//
LOCUS       DASUDV010000485         6556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1783788,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000485 DASUDV010000000
VERSION     DASUDV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783788"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..835
                     /locus_tag="VFV30_10490"
     CDS             137..835
                     /locus_tag="VFV30_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4848560.1"
                     /translation="MNFHGEDLRKIYRTGETEVHALRGVDLDIEAGEVLVLLGPSGSG
                     KSTLLNILGGLDRPTSGRLTSGDLDLTALDDAALTRFRRDRVGFIFQFYNLIPSLTAE
                     ENVRLVTDIAENPMDPARALELVGLGERRDHYPAQLSGGEQQRVAVARAIAKQPGVLL
                     CDEPTGALDSATGVRVLEALEAANRETGATLVIITHNAGIAAMADRVFHFLDGRIARI
                     ERNAVRTAPGKMTW"
     gene            832..3195
                     /locus_tag="VFV30_10495"
     CDS             832..3195
                     /locus_tag="VFV30_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4848561.1"
                     /translation="MKALDRKLLRDLWHMRGQVFAIALVLAAATATFVLSMGVHRSLV
                     ETRDAYYARNNFAEVFAGLTRAPRSLVERVQAIPGVRKAEGGIIQYATLDFPDRPEPV
                     RALINSVNEDGRTELNRLTLRSGRLPRPGAAGEVVADESFVKANGLQLGSRITALIYG
                     KQQVLTVVGTGLAPNYVYALPPGDILPDDRRFGIFWMGEKALEAATDRSEAINSLSLT
                     LERDAKEANVIRQLDALLAPYGGTGAYGREDHLSHAFLDNELVQLNAMTRVIPPVFLL
                     VATFLVYIVLGRMIRTERTQIGLIKAFGYTNAEVGWHYLKFALAIAVVATVLGCASGV
                     WMGRAMTRIYAEYYRFPFLTYLLDPRVFLAAAGLSFGAAALGALGGMRAALVLTPAVA
                     MTAPPPPVYRRGLVEALGRRAGFSAVGHMIVRHIARWPGRAAITVFGVGLSVGLLFAT
                     TQFLDSSRAMLDGFFFRAQKQDLMVSFIEPRSEAALFALAQIPGVLRVEPSRAVAVRL
                     SHGNRVERGAIESAPPGAQLSAHIDIAGNAIDLPASGLMLSRQLADKLGVRPGDTVWV
                     EELGGRRSRQALPVARVIEEFVGERAYASEATLLGITRDAAPVGSALLRIDPAGRDAI
                     LIRLKDMPMVLGVSERATTFSRFEDMIDENINTMLAFYISFAGAIAVGVVYNSARILF
                     SERAHELATMRVLGYHRREVATVLLGELALLVVMAVPLGCLLGNGLARVMTAMFSSDL
                     FRLPFAPEPASYGRAVLIVLAATALTSLLVVRRVMNLDMVRVLKGRD"
     gene            3195..4349
                     /locus_tag="VFV30_10500"
     CDS             3195..4349
                     /locus_tag="VFV30_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4848562.1"
                     /translation="MAALLKRWRWALLIAALLVAGLAFAFWPRAVLVDAGQVSRGRMV
                     TGITDDGQTRAVDVYVVSAPVTGYLDRIELEAGDTVAAGSVITRIKGRPSTPLDPRSR
                     QELTAARSAAQAAESGARAALDQSWRDLQRAEKLAKDGFLPRAQLETARTRVAGDRAA
                     LAAAQAEVRRIEAQLGAPTGLASGTPVPVRAPAGGSVLYVINESAGVVAEGTPIMTIG
                     DPERIEGVVDLLSREAVRVKPGDAVEITQWGGPKPLRGFVKRIEPFGRLKVSALGIEE
                     QRVNVIIGFAPESLPDAARLGHGYQFDATFVLWSREDALRLPIGALFRGTDGEWHAFE
                     IVGGRAADRKLTIGQINEEWGEVLGGLSEGAQVVVNPSKTLTDRTRVKAR"
     gene            complement(4346..5131)
                     /locus_tag="VFV30_10505"
     CDS             complement(4346..5131)
                     /locus_tag="VFV30_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4848563.1"
                     /translation="MIDTAQALLAALSGVAVGFTLGLVGGGGSILAVPLMVYVVGVRS
                     PHLAIGTSALAVAANALAGLANHARAGNVNWRCGGTYALAGVIGAFAGSTLGKAVDGQ
                     KLLFLFALLMLVVAFLMFRGRGEEGIEGVVCNRDNFPKVASFGLGTGALSGFFGIGGG
                     FLIVPGLIASTSMPIYRAIGTSLVAVSAFGITTALNYAASGLLDWAIAGAFIAGGMAG
                     SVAGSRAAKALAGSKGTLSTVFATMIVFVALYTLWKSGQAMGF"
     gene            complement(5128..6051)
                     /locus_tag="VFV30_10510"
     CDS             complement(5128..6051)
                     /locus_tag="VFV30_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4848564.1"
                     /translation="MTDPVLVQAIQHVEAARASGQVPVVKTFFDEPTFTATHVVHDPK
                     TRRAAIIDSVLDFDQPSGRTTTASAEAVVAYVQAEGLSVDWIIETHAHADHLSAAPFL
                     KERLGGAVVIGTAITTVQQTFAKVFNEPASFAQDGSQFDRLMGDGERFMLGEIPAIAL
                     HVPGHTPACLAYVIGDSVFVGDTLFMPDYGTARCDFPGGDARTLYRSIRRLMELPGET
                     RVFLCHDYKAPGRADYVFETTIAAERAGNIQVHEGVAEDDFVTMREARDATLAMPRLI
                     LPSVQINIRAGHLPEPEENGVRYLKLPLNLL"
     gene            6244..>6556
                     /locus_tag="VFV30_10515"
     CDS             6244..>6556
                     /locus_tag="VFV30_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4848565.1"
                     /translation="MTKPADLAELKANAAAMAGRLKIMSHPERLIMLCRMDEGEASVT
                     ELVALSGLSQSAVSQHLALLREEGIVASRGEAQTRYYSLKDPVVRAIIHALCDICAET
                     GG"
BASE COUNT         1035 a         2198 c         2320 g         1003 t
ORIGIN      
        1 gcctgaagcg cgatctcgcc gcgatcgagg accggcgcca gcgcgaggcg gcgatcgcgg
       61 cgatgcgcaa ggtgctggaa agctataccg cgcaccgcgc ggaccagcgc cgccggcggc
      121 ggggaccgca ccgcaaatga acttccacgg ggaagatctg cgcaagatct accggaccgg
      181 ggaaaccgag gtgcacgccc tgcgcggggt cgatctggat atcgaggcgg gcgaagtgct
      241 ggtcctgctg ggcccttcgg gcagcggcaa atcgaccctg ctcaacatcc tgggcgggct
      301 cgaccggccg acttcgggcc ggctgaccag cggcgatctg gacctgaccg cgctggacga
      361 tgccgcactg acccggttcc ggcgggaccg ggtgggcttc atcttccagt tctacaacct
      421 gatccccagc ctgaccgcgg aagagaacgt ccgcctcgtc accgacattg ccgaaaaccc
      481 gatggacccg gcccgcgcgc tggagctggt cggcctgggc gagcggcgcg atcactatcc
      541 ggcgcagctg tccggcggag agcagcagcg cgtggccgtg gcccgggcga tcgccaagca
      601 gcccggcgtg ctgctttgcg acgagccgac cggcgcgctc gacagcgcga ccggggtgcg
      661 ggtgctggaa gcgttggagg ccgccaaccg ggaaaccggg gccacgctgg tgatcatcac
      721 ccacaacgcc gggattgccg ccatggccga ccgggtgttc cattttctcg acgggcggat
      781 cgcccggatc gagcgcaacg cggtccgcac cgcgcccgga aagatgacct ggtgaaagcg
      841 ctcgaccgca agctgctgcg cgatctgtgg cacatgcggg ggcaggtctt tgcgatagcc
      901 ctggtgctgg cggcggcgac cgccaccttc gtcctgtcga tgggcgtcca ccgctcgctg
      961 gtggaaaccc gcgacgccta ttacgcccgc aacaatttcg ccgaggtctt tgccggcctg
     1021 acccgcgccc cgcgcagcct ggtcgaacgg gttcaggcaa tccccggcgt gcgcaaggcc
     1081 gaaggcggga tcatccagta cgcgacgctc gattttccgg accggcccga accggtgcgg
     1141 gcgctgatca attcggtcaa cgaggacggc cggaccgagc tcaaccggct gaccctgcgg
     1201 tccggccgcc tgccccggcc gggcgcggcg ggcgaagtgg tggccgacga atccttcgtg
     1261 aaggccaacg gcctgcaact gggcagccgg atcaccgcgc tgatctatgg caagcagcag
     1321 gtgctgacgg tcgtcggcac cggtctggcg cccaactatg tctatgccct gccgccgggc
     1381 gacatcctgc ccgatgaccg ccgcttcggg atcttctgga tgggcgaaaa ggcgctggag
     1441 gcggcgaccg accgcagcga ggcgatcaac agcctgtcgc tgacgctgga gcgcgatgca
     1501 aaagaagcga acgtgatccg ccagctcgac gcgctgctcg cgccctatgg cgggaccggg
     1561 gcctatggcc gcgaggatca cctcagccac gccttcctcg acaacgaact ggtccagctg
     1621 aatgcgatga cccgggtgat cccgccggtg ttcctgctgg tcgcgacctt cctggtctac
     1681 atcgtgctgg gccggatgat ccggactgag cggacccaga tcgggctgat caaggccttc
     1741 ggctacacca atgccgaagt cggctggcac tacctgaaat tcgccctggc gattgccgtg
     1801 gtggcgaccg tgctgggctg cgccagcggg gtgtggatgg gccgggcgat gacccggatc
     1861 tatgccgagt actaccgctt cccgttcctg acctacctgc tcgacccgcg ggtgttcctg
     1921 gcggcggccg ggctttcgtt cggcgcggcg gcgctggggg cgctgggcgg catgcgcgcc
     1981 gcgctggtgc tgaccccggc cgtggcgatg accgcaccgc ccccgccggt ctaccgccgc
     2041 gggctggtcg aggcgctggg gcggcgggcc ggcttttccg ccgtgggcca catgatcgtg
     2101 cggcacatcg cccgctggcc gggccgcgcg gccatcaccg tattcggggt cggcctctcg
     2161 gtcggcctgc tgttcgccac cacccagttc ctcgattcga gccgggccat gctcgacggg
     2221 ttcttcttcc gcgcgcagaa gcaggacctg atggtctcgt tcatcgaacc gcgcagcgag
     2281 gcggcgctgt ttgccctggc ccagatcccc ggcgtgctgc gggtcgagcc gagccgcgcg
     2341 gtggcggtgc ggctgagcca cggcaaccgg gtggagcgcg gcgcgatcga aagcgcgccg
     2401 cccggtgcgc agctttcggc ccatatcgac attgcaggca acgcaatcga tctccccgca
     2461 tcggggctga tgctgagccg ccagctggcc gacaagctgg gcgttcgccc cggcgacacg
     2521 gtgtgggtgg aggaactggg cgggcgccgc tcgcgccagg ccctgcccgt ggcgcgggtg
     2581 atcgaggagt tcgtgggtga gcgcgcctat gccagcgagg cgaccctgct gggaattacc
     2641 cgcgatgccg ccccggtcgg ctccgccctg ctgcggatcg atcccgccgg gcgcgatgcg
     2701 attctgatcc ggctcaaaga catgccgatg gtgctgggcg tatcggagcg ggcgacgacc
     2761 ttttcccggt tcgaggacat gatcgacgag aacatcaaca ccatgctggc cttctacatc
     2821 agctttgccg gagcgatcgc ggtgggggtg gtctacaatt cggcgcgcat cctgttttcc
     2881 gagcgggccc acgagctggc gaccatgcgg gtgctgggct atcaccgccg cgaagtggca
     2941 accgtgctgc tgggcgaact ggcgctgctg gtggtcatgg cggtgccgct gggctgcctg
     3001 ctgggcaacg gcctggcccg ggtgatgacc gcgatgttca gttccgacct gttccgcctg
     3061 cccttcgcgc ccgaacccgc cagctatggc cgggccgtgc tgatcgtgct ggcggcgacc
     3121 gcgctgacat cgctgctggt ggtgcggcgg gtcatgaacc tcgatatggt ccgcgtgttg
     3181 aagggacggg attgatggcg gcattgctga aacgctggcg ctgggcgctg ctgatcgcgg
     3241 ccttgctggt ggcgggactg gcctttgcct tctggccgcg ggcggtgctg gtcgatgccg
     3301 ggcaggtatc gcggggccgg atggtaaccg ggatcaccga cgacggacag acccgcgcgg
     3361 tcgatgtcta tgtcgtttcc gccccggtca ccggctatct tgaccggatc gagctggagg
     3421 ccggagacac ggtggcggcg ggcagcgtga tcacccggat caagggccgc ccctcgaccc
     3481 cgctcgatcc gcgcagccgc caggaactga ccgccgcccg gtccgccgcc caggcggccg
     3541 agagcggcgc gcgcgccgcg ctcgaccagt cctggcgcga cctgcaacgc gccgaaaagc
     3601 tggcgaagga cgggttcctg ccccgcgccc agctggaaac cgcgcgcacc cgcgtggccg
     3661 gcgaccgggc cgccctggcc gcggcccagg ccgaagtgcg gcggatcgag gcccagcttg
     3721 gtgcgccgac cggcctggcc agcggcaccc cggtgccggt ccgcgccccg gccggcggat
     3781 cggtcctcta tgtgatcaac gaaagcgccg gcgtggtggc ggaaggcacc ccgatcatga
     3841 ccatcggcga tcccgagcgg atcgagggcg tcgtcgacct gctctcgcgc gaggcggtgc
     3901 gggtgaagcc cggcgatgcg gtggagatca cccagtgggg cggccccaaa ccgctgcgcg
     3961 gcttcgtcaa gcggatcgag ccgttcgggc ggctcaaggt ttcggcgctg gggatcgagg
     4021 agcagcgggt caacgtgatc atcggcttcg cgccggaatc gctgcccgat gcggcgcggc
     4081 tgggccacgg ctatcagttt gacgcgacgt tcgtgctgtg gagccgcgag gatgccctgc
     4141 gtctgccgat cggcgcgctg tttcgcggca cggacggcga atggcacgct ttcgagatcg
     4201 tcggcggaag ggcggccgac cgcaagctga cgatcggcca gatcaacgag gagtggggcg
     4261 aagtgctggg cggcctctcc gaaggcgcgc aggtggtggt caatccgtcg aagacgctga
     4321 cggaccgtac gcgggtgaaa gcgcgctaga accccatcgc ctgtccgctc ttccacaggg
     4381 tgtagagcgc aacgaagacg atcatcgtcg cgaacacggt gctcagcgtg cccttgctgc
     4441 cggccagtgc cttggcggcg cggcttcccg ccacgctgcc cgccatgccc ccggcgatga
     4501 aggccccggc aatggcccag tcgagcagac cggaagcggc atagttgagc gcggtggtga
     4561 tcccgaaggc gctgaccgcg accagcgagg tgccgatcgc gcggtagatc ggcatcgagg
     4621 tggacgcgat cagccccggc acgatcagaa agcccccgcc gatcccgaag aagcccgaca
     4681 gcgccccggt gcccaggccg aacgaggcga ccttggggaa attgtcgcgg ttgcagacca
     4741 cgccctcgat tccctcctcg ccccgcccgc ggaacatcag gaaggcgacg accagcatca
     4801 gcagggcaaa caggaagagc agcttctgcc catccaccgc cttgcccagc gtcgatccgg
     4861 caaaggcccc gatcaccccg gccagggcat aggtcccgcc gcagcgccag ttgacgttgc
     4921 cggcccgggc atggttggca agccccgcca gggcattggc cgccacggcc agcgccgaag
     4981 tgccgatcgc caggtgcggg cttctcaccc ccaccacata gaccatcagc ggcacggcca
     5041 ggatcgagcc gcccccgccg acgaggccaa gggtaaagcc cacggcaacg ccgctcagcg
     5101 cggcaagcag ggcctgggcg gtgtcgatca cagcaggttc agcggcagct tgaggtagcg
     5161 gacgccgttc tcctccggct cgggcagatg cccggcgcgg atattgatct gaaccgaggg
     5221 caggatcagc cgcggcatgg cgagcgtcgc gtcgcgcgcc tcgcgcatgg tcacgaaatc
     5281 gtcctcggcc acgccttcgt gcacctggat gttgccggcg cgttcggccg cgatggtggt
     5341 ttcgaagaca tagtcggccc ggcccggcgc cttgtaatcg tggcacagga acacccgggt
     5401 ttccccgggc agctccatca gccgccggat cgagcggtag agcgtgcgcg catcgccgcc
     5461 cgggaaatcg cagcgggccg tgccgtagtc cggcatgaac agggtatcgc caacgaatac
     5521 cgaatcgccg atcacatagg ccaggcaggc cggggtgtgg ccgggcacgt ggagcgcgat
     5581 ggccgggatt tcgcccagca tgaaccgctc gccatcgccc atcaggcggt cgaactgcga
     5641 cccgtcctgg gcgaagctgg ccggttcgtt gaagaccttg gcgaaggtct gctggaccgt
     5701 ggtgatcgcc gtgccgatca ccaccgcccc gcccaaccgc tccttcagga acggcgcggc
     5761 cgagaggtgg tcggcatggg catgggtctc gatgatccag tcgacgctga gcccctcggc
     5821 ctggacatag gccaccaccg cctcggccga agcggtggtc gtgcggcccg aaggctgatc
     5881 gaaatcgagc accgaatcga tgatcgccgc gcgccgggtc ttcggatcgt ggaccacgtg
     5941 ggtggccgtg aaggtcggct cgtcaaagaa ggtcttcacc accggcacct ggccagaggc
     6001 gcgggcggct tcgacgtgct ggatggcctg gacaaggacg ggatcggtca tggcgtcact
     6061 ctcggatcgg cgggttgccc tgtgccgtca cggtgggggc tgtgacggca caggaccggg
     6121 caggcacgaa cgtgcgcgcc gtgtttgaat tctatattgc ttatataagg attgcaatat
     6181 ataaatcgcg tcctgcctgc cggggccctt gcggaccgcc cggcactggc cctaggagaa
     6241 ggcatgacca agcccgccga ccttgccgaa ctgaaggcca acgccgctgc catggcaggg
     6301 cggctcaaga tcatgagcca cccggaacgg ctgatcatgc tgtgccggat ggacgaaggc
     6361 gaagcttcgg tgacggaact ggtggcgctg tccggcctgt cgcaatcggc cgtatcgcag
     6421 cacctggccc tgctgcgcga ggaagggatc gtcgcaagcc ggggcgaggc ccagacccgc
     6481 tattacagcc tgaaggaccc ggtggtccgc gcgatcatcc acgcgctctg cgatatctgc
     6541 gccgagaccg gcgggt
//
LOCUS       DASUDV010000486         7675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_82755,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000486 DASUDV010000000
VERSION     DASUDV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82755"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1015
                     /locus_tag="VFV30_10520"
     CDS             56..1015
                     /locus_tag="VFV30_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848566.1"
                     /translation="MPEFRMIDTGEVTLRCAVEGSGPLAIMVHGFPESWYSWRHQIGP
                     VAAAGFTACAIDVRGYGGSDKPHPVEAYTLEAIAGDLIGLADALSPNQPVVLIGHDWG
                     APIVWNTAFTNPDRIRAVAGLSVPFAGAPLRPFTEVFREHFTSQGRFFYQEYFQEPGV
                     AEAEAEADPRDFVARMMYSISGDVPPGDYWSKPAGATFLQGLPDPQPVPWLSEADLDY
                     YEGEFKASGFRGPLNRYRNHERDYEWLQPWQGKQLEQPCLFIGGTRDPATTLFGAVAD
                     PVAMMRMFAPRVEGHVLDGVGHWTQQERPEAVNALLVDWLKRL"
     gene            1108..2019
                     /locus_tag="VFV30_10525"
     CDS             1108..2019
                     /locus_tag="VFV30_10525"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX4848567.1"
                     /translation="MSTAPAQPIPLPADWRDFLSLTKPRVMSLVIFTGLCGMLAAPVP
                     VDPVIGFVAILCIAVGAGGSAALNQWWEADLDAKMKRTAARPLPAGRMERGSARDFGV
                     ILSVGSVLTMGLAVGWLAAAILAFSIFYYAVIYTIWLKPRTPQNIVIGGGAGAFPPMI
                     GWIAATGEVTLMPVLLFAIIFFWTPPHFWALALFVETDYAKAGIPMMPVVAGHQSTRR
                     QILIYAVLMLPIALAPFWLEVAGWLYGVAALLLTAVFLLLSLRVAVFRGGASDADMRP
                     EKRLFGYSVFYLFALFAALVADRYLVG"
     gene            2024..2167
                     /locus_tag="VFV30_10530"
     CDS             2024..2167
                     /locus_tag="VFV30_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848568.1"
                     /translation="MDPEGPDQVPDAMAERKRIIAGRNRVLALVLLALVALFFAITIV
                     KMK"
     gene            2175..2762
                     /locus_tag="VFV30_10535"
     CDS             2175..2762
                     /locus_tag="VFV30_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX4848569.1"
                     /translation="MNANKRTGLIAAGGAAAMLALGFASVPLYRLFCQVTGFGGTTQK
                     ADLEEAARVQVGTTTISVRFDSNTDPGLPWKFAPMQVSQNMPIGSRQLAFYRAENVSD
                     KPVTGVATFNVQPDIAGIYFKKVHCFCFNQQTLKPGEAVEMPVQYYVDPAILKDPEAA
                     GIKQITLSYTFHPSADQQPAGKVAANGLDPVPAAR"
     gene            2827..3657
                     /locus_tag="VFV30_10540"
     CDS             2827..3657
                     /locus_tag="VFV30_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX4848570.1"
                     /translation="MAGSKNHQYHILDPDWLPLVGSISAVTMTSGGVMAMHPAELGGL
                     GKYVLLLGFTGILFTMYVWWTKVVREANEGHHTPVVQLHLRYGMITFIASEVMFFVGW
                     FWAFFDFALFPNAISEVTGGQFPSKAIEAVMNPFDLPLLNTLILLCSGTTVTWAHHSL
                     LHGDRDGLKKGLWLTIALGVLFSAIQAYEYAHAPFGFGGNTYSSAFYMATGFHGFHVL
                     VGTIFLIVCLKRAYNGDFTPKQHFGFEAAAWYWHFVDVVWLFLFVAVYVWGGWGYAVH
                     "
     gene            3657..4124
                     /locus_tag="VFV30_10545"
     CDS             3657..4124
                     /locus_tag="VFV30_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX4848571.1"
                     /translation="MSKPTPNPSRLREGSETSRAEGEVSRSGEGNPSGQPGLGQAALF
                     GLCPRCGAKGLFAGGAKFAPRCGACGLDYSTFNVGDGPAAFLTMVIGAVVVGLALWVE
                     FTFHPPLWVHAVLWIPFVAGTTLWGLRVSKAALLAAEYLRHAREATTDDVRKD"
     gene            4239..4871
                     /locus_tag="VFV30_10550"
     CDS             4239..4871
                     /locus_tag="VFV30_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEX4848572.1"
                     /translation="MELDVTLLKRLPLIPTVIVVAAVLVMIRLGLWQVERMHEKDALL
                     LRYAQAQNLPVIRYDGTIGDPRNLFRRVEGTCRRVEGGGMMAGRNAAGDVGWAHAANC
                     AAIAPAKPGEEQMDGWKSAEVQVVIGWSREPREVRWAGGPYTGVLAPGAGDSVRVIAD
                     PPLAGLEANAKPDPRDIPNNHWSYAIQWFLFALVALVIYGLALRKRLAGA"
     gene            4944..6344
                     /gene="thrC"
                     /locus_tag="VFV30_10555"
     CDS             4944..6344
                     /gene="thrC"
                     /locus_tag="VFV30_10555"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX4848573.1"
                     /translation="MDYISTRGSAPALDFQGATLAGLASDGGLYVPREWPRFSRDEIA
                     AMAGLPYAELAARIMQRFVGDSLTPERLRELTAQAYGRFAHAAVTPLKQLDEQQWLLE
                     LFHGPTLAFKDVALQLLGLLFEEFLSRGGDPLTIVGATSGDTGSAAIDSVAGRARVDI
                     FMLHPKGRVSDVQRRQMTTVLAPNVHNIAIEGTFDDAQAHVKRMFNDTAMTSRFAIGA
                     VNSINWARLMAQVVYYFAAGLQLGAPHREVAFSVPTGNFGDVFAGYVAAQMGLPVARL
                     IVATNVNDILHRALTNGDYSAGTVTPTAAPSMDIQVSSNFERLLFDLNGRDGAALAAQ
                     MAGFEATRAMQLTNAQREGAAKLFSSARADADDMAQAIRWAHENAGELLDPHTAIGLH
                     AAQAAGLPREVPVVTLATAHPAKFRDAVERATGHRPALPARIGDLFEREERLVELPGD
                     YAAIADYVAAHAVQRG"
     gene            6344..7234
                     /locus_tag="VFV30_10560"
     CDS             6344..7234
                     /locus_tag="VFV30_10560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848574.1"
                     /translation="MAELVLDPVIMAGEGWADYGLVDCGHGRKYERYGPYSFIRPEPQ
                     AMWTPRLEHWPADGEFVPGSDEDGGGRWAYGTRPVPREGWTLARGPVQFTAQCTPFRH
                     LGFFPDMAPVWDWMGQRLDGRSDAQTMNLFGYTGVGTLALSEYGPVTHVDASKKSVTQ
                     ARANAALSGMEARPVRWIIDDAAKFAAREVRRGKRYDGIILDPPKFGRGPEGEVWRLE
                     EHLPGLVADCRRLLDADSRFLFLTVYAVRMSSLALAGLMDEIFRDLPGRIEHGDLAVR
                     EIGSDGRLLPTAIFARWSND"
     gene            7237..7608
                     /locus_tag="VFV30_10565"
     CDS             7237..7608
                     /locus_tag="VFV30_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX4848575.1"
                     /translation="MADSLTLEVANFEHDVLTGIYSEETGRPQPLRFTITVRLKPAER
                     YEPDTPLDASKNYMDLKFAASSALPEGVHFKLIEAVADHVCDTLFLQDPRVEAVTVKI
                     VKLAIAENGEDIGITLTRERR"
BASE COUNT         1163 a         2545 c         2609 g         1358 t
ORIGIN      
        1 cccgcgtgcg ggaggggagt tgacaggccg ccggctgcgc ggcgggagtg ttcctatgcc
       61 cgaattccgc atgatcgata ccggcgaggt gaccttgcgc tgcgccgtcg agggaagcgg
      121 cccgctggcg atcatggtcc acggctttcc ggaaagctgg tattcgtggc ggcaccagat
      181 cggcccggtg gctgcggccg gctttaccgc ctgcgcgatc gacgtgcgcg gctatggcgg
      241 ctcggacaag cctcacccgg ttgaagccta cacgcttgag gcgatcgccg gcgatctgat
      301 cgggttggcc gatgcgcttt ctccgaacca gccggtggtg ctgatcgggc acgattgggg
      361 cgcgccgatc gtctggaaca cggctttcac caatcccgac cggatccgcg ccgtggccgg
      421 tctctcggtc ccctttgccg gcgcgccgct gcgccccttt accgaggtgt tccgcgagca
      481 tttcaccagc cagggccggt tcttctacca ggagtatttc caggaacccg gcgtggccga
      541 ggcggaggcc gaggcggacc cgcgcgattt cgtggcccgg atgatgtatt cgatctccgg
      601 cgatgtgccg ccgggtgact attggtccaa gccggccggg gcgaccttcc tgcagggcct
      661 gcccgatccg cagccggtcc cctggttgag cgaggccgac ctcgattact acgagggcga
      721 gttcaaggcc tcgggctttc gcgggccgct caatcgctac cgcaaccacg agcgcgacta
      781 cgaatggctg cagccctggc agggcaagca gctggagcag ccctgcctgt tcatcggcgg
      841 cacccgcgat ccggcgacca cgctgttcgg cgcggtggcc gatccggtgg cgatgatgcg
      901 gatgttcgcg ccccgggtgg aaggccacgt gctcgacggc gtcggccact ggacccagca
      961 ggaacggccc gaggcggtga acgccctgct ggtggactgg ctgaagcggc tctgacgtcc
     1021 cgcgcgggcg gccacccgaa agtgcagggg cggaggacgg aatccgcctt ccaagcaccg
     1081 gcaagcccga ctatagggcg ctcagccatg agcaccgctc ccgcccagcc gattcccttg
     1141 cccgccgact ggcgcgattt cctgtcgctg accaagccgc gggtgatgag cctggtgatc
     1201 ttcaccggcc tgtgcggaat gctggcggcg ccggtgccgg tcgatccggt gatcggcttt
     1261 gtcgcgatcc tgtgcatcgc cgtgggggcg ggcggttcgg ccgcgctgaa ccagtggtgg
     1321 gaagccgatc tcgacgccaa gatgaagcgc accgccgcgc ggccgctgcc ggccggacgg
     1381 atggagcggg gctcggcgcg cgatttcggg gtgatcctgt cggtcggatc ggtgctgacc
     1441 atggggctgg ccgtgggctg gctggccgcg gcgatcctgg ccttttcgat cttctactat
     1501 gcggtgatct acaccatctg gctgaagccg cgcacgccgc agaacatcgt gatcggcggc
     1561 ggggccggag cctttccgcc gatgatcggc tggatcgcgg cgaccggcga agtgacgctg
     1621 atgccggtcc tgctgttcgc gatcatcttc ttctggaccc cgccgcattt ctgggcgctc
     1681 gccctgtttg tcgagaccga ctatgccaag gccgggatcc cgatgatgcc ggtggtggcc
     1741 gggcaccagt ccacccgccg ccagatcctg atctatgccg tgctgatgct gccgatcgcg
     1801 ctcgcgccgt tctggctgga agtggccggg tggctctatg gcgtcgcggc gctgctgctg
     1861 accgcggtgt tcctgctgct gtccctgcgg gtcgcggtgt tccgcggcgg cgcctccgat
     1921 gccgacatgc ggcccgaaaa gcggctgttc ggctattcgg tgttttacct ctttgccctg
     1981 ttcgccgcgc tggtggcgga ccgctatctg gttggatgac gcgatggacc ccgaaggacc
     2041 cgatcaagtt cccgacgcca tggccgaacg caagcgcatc attgccgggc gcaaccgcgt
     2101 tctggccctg gtgctgctgg ccctggtcgc gctgttcttc gcgatcacca tcgtcaagat
     2161 gaagtaggca aaccatgaac gccaacaagc gtaccggatt gatcgccgcc ggcggggccg
     2221 cggccatgct ggccctcggc tttgcttccg ttccgctcta ccgcctgttc tgccaggtga
     2281 cgggctttgg cggcaccacc cagaaggcgg acctggaaga ggccgcgcgg gtgcaggttg
     2341 gcaccaccac catttcggtg cggtttgaca gcaacaccga tcccggcctg ccgtggaagt
     2401 tcgcgccgat gcaggtcagc cagaacatgc cgatcggctc gcgccagctg gccttctacc
     2461 gggccgagaa cgtgtcggac aagccggtga ccggggtggc gaccttcaac gtccagcccg
     2521 atattgccgg gatttatttc aagaaggtcc actgcttctg cttcaaccag cagaccctga
     2581 aaccgggcga ggcggtggaa atgccggtcc agtactatgt cgatccggcg atcctgaagg
     2641 acccggaagc ggccgggatc aagcagatca cgctcagcta caccttccac ccttcggccg
     2701 atcagcagcc ggcggggaaa gtggctgcaa atgggctgga cccggtgcca gcggcgcgat
     2761 aagggcggga agattcaggg ggcggcaggc cgcccggcaa ggttttgacg agggaactac
     2821 agcaccatgg ccggtagcaa gaaccaccag taccacatcc tcgatcccga ttggcttccg
     2881 ctggttggtt cgatctccgc ggtgacgatg accagcggcg gcgtgatggc gatgcacccg
     2941 gccgaactgg gcgggctggg caagtacgtg ctgctgctcg gcttcaccgg catcctgttc
     3001 accatgtacg tgtggtggac caaggtggtg cgcgaagcca acgaaggcca ccacaccccg
     3061 gtggtgcagc tgcacctgcg ttatggcatg atcaccttca tcgcctcgga agtgatgttc
     3121 ttcgtcggct ggttctgggc gttcttcgat ttcgccctgt tccccaacgc gatttccgaa
     3181 gtgaccggcg ggcagttccc gtccaaggcg atcgaagcgg tgatgaaccc gttcgatctg
     3241 ccgctgctca acacgctgat cctgctgtgc tcgggcacca cggtgacctg ggcccaccac
     3301 agcctgctgc acggtgaccg cgatggcctg aagaagggcc tgtggctgac catcgcgctc
     3361 ggcgtgctgt tcagcgcgat ccaggcctac gaatacgccc acgcgccgtt cggctttggc
     3421 ggcaacacct acagctcggc gttctacatg gcgaccggct tccacggctt ccacgtgctg
     3481 gtcggcacga tcttcctgat cgtctgcctc aagcgcgcct acaacggcga tttcaccccc
     3541 aagcagcact tcggtttcga agccgccgcc tggtactggc acttcgtcga cgtggtctgg
     3601 ctgttcctct tcgtcgccgt ctatgtctgg ggtggctggg gctacgcggt ccactgatgt
     3661 ccaaacccac ccccaacccc tcccgcctgc gggaggggag cgagacttcc cgagccgaag
     3721 gcgaggttag tcgcagcggg gagggcaatc cttcagggca gcccggtctc ggccaggctg
     3781 cccttttcgg tctctgcccc cgttgcggag ccaagggcct gtttgccggc ggtgccaagt
     3841 tcgccccgcg ctgcggcgcc tgcgggctcg attactccac cttcaatgtc ggcgatggcc
     3901 cggccgcgtt cctgaccatg gtgatcggcg cggtggtggt ggggctggcg ctgtgggtgg
     3961 agttcacttt ccacccgccg ctgtgggttc acgcggtgct gtggatcccg ttcgtggcgg
     4021 gaaccacgct gtggggcctg cgggtcagca aggccgcgct gctggcggcc gaatacctgc
     4081 gccacgcccg cgaggcgacg accgacgatg tgcggaagga ctgacccttc cccaaagtcc
     4141 gtccgtcctg agcgtgccga aggactgtcc ttttccgcgc ggctggcgca tggtcacaga
     4201 gtgaaagcag cccttgggca ggctcagggc gaacggtgat ggaactggat gtgacccttc
     4261 ttaaacggct tcccctgatc ccgaccgtca tcgtcgttgc tgcagtcctg gttatgatcc
     4321 ggctgggcct gtggcaggtt gagcggatgc acgagaagga cgcgctgctg ttgcgctatg
     4381 cgcaggcgca gaacttgccg gtgatccgct atgacggaac gatcggcgac ccccgcaacc
     4441 tgttccgccg ggttgagggc acctgccgcc gggttgaagg cggcggcatg atggccgggc
     4501 gaaacgccgc cggggacgtg ggctgggcgc acgccgccaa ctgcgctgca atcgctccgg
     4561 ccaagccggg cgaggaacag atggacggct ggaagtcggc cgaagtgcag gtcgtgatcg
     4621 gctggagccg cgaaccgcgc gaagtgcggt gggcgggcgg accctatacc ggcgttctcg
     4681 ccccgggtgc aggcgattcc gtccgggtca tcgccgatcc gccccttgcc gggctggagg
     4741 ccaacgccaa gcccgatccg cgcgacatac ccaacaacca ctggtcctat gcgatccagt
     4801 ggttcctctt cgcgctggtc gcgctggtca tctacggcct ggccctgcgc aagcggctgg
     4861 cgggcgcata gcatttttcg ataggcccgc gcggccttgc agggtttcca tttgcggccc
     4921 ggccagctat cgcgcggctc gcaatggact atatcagcac ccgtggcagc gctcccgcgc
     4981 tcgactttca aggcgcaacg ctggccgggc tggccagcga tggcggtctc tatgtgccgc
     5041 gcgaatggcc gcgcttcagc agggacgaga tcgcggccat ggccggtctg ccctatgcgg
     5101 agcttgccgc gcggatcatg cagcgcttcg tgggcgacag cctgacgccg gagcggctgc
     5161 gcgaactgac cgcccaggcc tatggccgtt ttgcccacgc cgcggtcacc ccgctgaagc
     5221 agctggacga gcagcagtgg ctgctggagc tgttccacgg cccgacgctg gccttcaagg
     5281 acgtggcgct gcaactgctg ggcctgctgt ttgaggaatt cctgtcgcgc ggcggcgatc
     5341 cgctgaccat cgtcggggcc acttcgggcg ataccggttc ggccgcgatc gattccgtcg
     5401 cgggccgcgc ccgggtcgat atcttcatgc tccaccccaa ggggcgggtt tcggatgtgc
     5461 agcgccggca gatgaccacg gtgctggccc ccaacgtcca caacatcgcg atcgagggca
     5521 cgtttgacga tgcccaggcc cacgtgaagc ggatgttcaa cgacaccgcc atgaccagcc
     5581 gcttcgcgat cggtgcggtc aattcgatca actgggcgcg gctgatggcg caggtggtct
     5641 attacttcgc cgccgggctt cagctcggcg cgccgcaccg cgaagtggcc ttctcggtcc
     5701 cgaccggcaa tttcggcgat gtcttcgcgg gctatgtcgc ggcgcagatg ggcctgccgg
     5761 tggcgcggct gatcgtggcg accaacgtca acgacatcct ccaccgcgcg ctgaccaatg
     5821 gcgactattc ggccggcacg gtcacgccca cggcggcgcc gtcgatggac atccaggtct
     5881 cgtccaattt tgaacgcctg ctgttcgatc tcaacgggcg cgacggcgcg gcgctggccg
     5941 cgcagatggc gggctttgaa gcgaccaggg cgatgcagct gaccaacgcc cagcgcgaag
     6001 gcgcggccaa gctgttttcc agcgcgcggg ccgatgccga tgacatggcc caggcgatcc
     6061 gctgggcgca tgagaacgcg ggcgagctgc tcgatccgca caccgcgatc ggccttcacg
     6121 ccgcccaggc cgccggcctg ccgcgcgagg ttccggtggt gacgctggcc acggcccacc
     6181 cggccaagtt ccgcgacgcg gtggaacgcg ccaccggcca ccgcccggcg ctgcccgcgc
     6241 ggatcggcga cctgttcgag cgcgaggaac ggctggtcga gctgccgggc gattacgctg
     6301 cgattgcaga ttacgtggcc gcccacgcgg tccagcgggg ctgatggccg aactcgttct
     6361 cgatcccgtg atcatggcgg gagagggctg ggccgactac ggcctggtcg attgcgggca
     6421 cggccgcaag tacgagcgct atggccccta cagcttcatc cgcccggaac cccaggcgat
     6481 gtggaccccg cggctggagc actggccggc cgacggtgaa ttcgtgcccg ggtcggacga
     6541 ggacggcggc gggcgctggg cctatggcac ccggcccgtg ccgcgcgagg gctggaccct
     6601 ggcccgcggg ccggtgcagt ttacggccca atgcaccccg ttccggcacc tcggcttctt
     6661 tccggacatg gccccggtgt gggactggat ggggcagcgg ctggacgggc gcagcgatgc
     6721 ccagacgatg aacctgttcg gctataccgg cgtcggcacc ctggccctgt cggaatacgg
     6781 gccggtcacc catgtcgatg ccagcaagaa gtcggtcacc caggcgcgcg ccaacgccgc
     6841 gctttcgggg atggaggcgc gcccggtgcg ctggatcatc gatgatgccg ccaagttcgc
     6901 cgcgcgcgaa gtgcggcggg gcaagcgcta tgacgggatc atcctcgatc cgcccaaatt
     6961 cggccgcggg ccggaaggcg aagtgtggcg gctggaagag catctgcccg gcctggtcgc
     7021 cgattgccgc cgcctgctcg atgccgacag ccgcttcctg ttcctgacgg tctatgccgt
     7081 gcggatgagc tcgctggcat tagccggatt gatggacgag atttttcgcg atcttcccgg
     7141 ccggatcgag cacggcgacc ttgccgttcg cgaaattggc tccgatggtc ggctccttcc
     7201 caccgcgatc ttcgcccgct ggagcaacga ctagccatgg ccgacagcct gactctcgaa
     7261 gtcgccaatt tcgagcacga tgtcctgacc gggatctact cggaagagac cggccgtccg
     7321 cagccgctgc gctttacgat cacggtccgg ctgaagccgg ccgagcgcta cgagccggac
     7381 acgccgctcg atgccagcaa gaattacatg gacctcaagt tcgcggcctc gtcggccctg
     7441 cccgaagggg tccacttcaa gctgatcgag gccgtggccg atcacgtctg cgacacgctg
     7501 ttcctgcagg acccgcgggt tgaggcggtg acggtcaaga tcgtcaagct ggcgattgcc
     7561 gaaaacggcg aggacatcgg catcacgctc acccgcgagc ggcgctgatg ggcacgctgg
     7621 cggtcagcgg cctgcccgat ggccccagcg ccgccgccgc gcagttccac gccgc
//
LOCUS       DASUDV010000487         3690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2114961,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000487 DASUDV010000000
VERSION     DASUDV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114961"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2220
                     /gene="putA"
                     /locus_tag="VFV30_10570"
     CDS             <1..2220
                     /gene="putA"
                     /locus_tag="VFV30_10570"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX4848576.1"
                     /translation="LLAYLVRRLLENGANSSFVHRIWDENVSVEELAADPVALARAVE
                     PLGAPHPVVALPREILGERRNSAGLDLSDENTLAELGDALRRSAQGSTCSRPIVAGQT
                     LDRPERPVLNPADHSDQVGLARFALPADAVAAVAAADAAVARWCAVPVDQRAACLERA
                     ADLMEARMPALLGLIVREAGKSLPNAIAEVREAVDFLRYYAAEARRTLGVETRPLGAV
                     VCISPWNFPLAIFTGQIAAALAAGNTVLAKPAEETPLIAAEGVRILHEAGIPAEALHL
                     LPGAGDVGAALVGAAQTAGVLFTGSTEVAKLIQAELAGHTAPHGAPIPLIAETGGQNA
                     MIVDSSALAEQVVFDVIASAFDSAGQRCSALRVLCLQDDVADRQLAMLKGALAELALG
                     RPDRLSVDIGPVISAEARWGIEDHIARMRAAGCRVEQLPLPSETARGTFVAPTIIEIE
                     SITQLSREVFGPVLHVLRWKRDELDALIDAINATGYGLTFGLHTRLDEVVERVTARAG
                     AGNVYVNRNVIGAVVGVQPFGGRGLSGTGPKAGGPLYLGRLVQSAPETGLAGQAPDPV
                     LGELAGWLEQHGEPGLAQRARSYRALNGHAAELPGPVGERNLYALHPRGRVLALPNSR
                     EGLLAQLVAALGEGNRVAVVWPGITLDTLPALVAQRIDWLPALPERSDFAAVLIEPRS
                     GGIAELLARVAAMDGPIPLVQMADAAGHYRADWLVEEVSVSINTTAAGGNASLMALV"
     gene            2332..>3690
                     /locus_tag="VFV30_10575"
     CDS             2332..>3690
                     /locus_tag="VFV30_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848577.1"
                     /translation="MDILGTSGNDILDGTEGDDVFNGLGGAQDTFSGLGGSDTFLFST
                     PVSFSNVYDGGAGTDWFELRTSVFAAIGQDGNLGSAYIVNASLTTSVEGVRFTGTVGE
                     TQQLVIASFFGGNAAAVPSLIEGSAGRDLLAFGAFGGMGGAAELVVPTLSASNWTGPG
                     AIYRDEGDAVLFYIGDANGYVLRANEVNAAAGIVQALVGGAGNDTLIGSAGADKLVSI
                     VGGNDALYGNGGNDSLAAINQAPNGSAAITTLTFAGSTFDGGDGTDFLSIGGRVNFQG
                     TLTSIEGINLLPQIAVANPSAPRREAAELTISSAQLASLPSNLQIDGTGNIAVTMVSG
                     AAFNGSLYQMLAGSDVNFTITGTSGNETITGTTGDDVILPAGGVDTIHADAGNDEIVL
                     TAPIGAGSILDGGIGYDTLVLRPQSTYPNATGGYSTQYNAYFPTQIQGLEAARFETNA
                     GDT"
BASE COUNT          603 a         1231 c         1230 g          626 t
ORIGIN      
        1 ctgctggcct atctggtccg ccgcctgctg gagaacgggg caaactcctc gttcgtgcac
       61 cggatctggg acgagaacgt ttcggtagag gaactggccg ccgatccggt tgcgctcgct
      121 cgcgcggtcg aaccgctggg agcaccccat ccggtggtcg ccctgccgcg cgaaattctg
      181 ggcgaacggc gcaattcggc cgggctcgat ctctcggacg agaacaccct cgccgaactg
      241 ggcgatgcgt tgcggcgctc ggcacagggc agcacttgtt cccggccgat cgttgcgggc
      301 cagacgctgg acaggccgga acgcccggtg ctcaatccgg ccgatcacag cgatcaggtt
      361 gggctggcca gattcgccct tcccgccgat gccgtcgccg cggtcgccgc cgccgatgcg
      421 gccgtggcga ggtggtgtgc cgttccggtc gatcagcgcg cggcctgcct tgaacgcgct
      481 gccgacctga tggaggcgcg catgcctgcg ctgctcggcc tgatcgtgcg cgaggctggc
      541 aagtcgctac ccaatgcaat cgccgaggtg cgcgaagcgg ttgacttcct gcgctactac
      601 gccgcggaag cacgccgcac gctgggcgtg gaaacccgcc cgctgggcgc ggtggtgtgc
      661 atcagcccgt ggaacttccc gctggcaatc ttcaccgggc agatcgccgc cgcgctggcc
      721 gcgggcaaca ccgtgctggc caaaccggcc gaggaaaccc cgctgatcgc cgccgagggc
      781 gtgcgcatcc tccacgaggc cggcatcccc gccgaggcgc tccacctgct tcccggcgcg
      841 ggcgatgtcg gcgcggcgct ggtgggtgcg gcgcagaccg caggcgtgct gttcaccgga
      901 tcgaccgagg ttgcaaagct gatccaggcg gaactcgccg gccacaccgc accgcacggc
      961 gcgccgatcc cgctgatcgc cgaaaccggc ggccagaacg cgatgatcgt cgacagttcg
     1021 gcgctggcgg aacaggtggt tttcgacgtg atcgcatcgg ccttcgacag tgccggtcag
     1081 cgctgttcgg ccttgcgcgt gctgtgcctg caggacgatg tggccgaccg ccagctggcc
     1141 atgctcaagg gcgcgctggc cgaacttgcg ctgggccggc ccgatcgcct ttcggtcgat
     1201 atcggcccgg tgatcagcgc cgaggcgcgc tgggggatcg aggatcatat tgcgcggatg
     1261 cgggccgcgg gctgccgggt agagcagttg cccctgcctt cggaaaccgc gcgcggcacc
     1321 ttcgttgccc cgacgatcat cgagatcgag agcatcaccc agctgagccg ggaggtgttc
     1381 ggcccggtgc tgcacgtgct gcgctggaag cgcgacgagc tggacgcgct gatcgatgcg
     1441 atcaacgcta ccggctatgg cctgaccttc ggcctgcaca cccggttgga cgaagtggtc
     1501 gaacgcgtca ctgcgcgggc gggcgcgggc aatgtctacg tcaatcgcaa cgtgatcggc
     1561 gcggtggtgg gcgttcagcc atttggcggg cgcggtcttt cgggcaccgg acccaaggcc
     1621 ggcgggccgc tctatctggg ccgtctggtg cagtccgcgc ccgaaaccgg gctggctggc
     1681 caggcgccag acccggtgct gggcgagttg gccggctggc tcgaacagca cggcgaacca
     1741 ggcctggccc agcgcgcccg cagctaccgc gcgctcaacg gccatgccgc ggaactgccc
     1801 ggaccggtgg gcgagcgcaa tctctatgcc ctgcatcccc gtggccgcgt gctggccctg
     1861 cccaacagcc gcgagggcct gctggcccag ctggtcgccg cgcttggcga aggcaaccgg
     1921 gtggcggtgg tctggccggg gatcaccctc gacaccctgc ccgcactagt ggcccagcgg
     1981 atcgactggc tgcccgccct gcccgagcgc agtgacttcg ccgccgtgct gatcgaaccg
     2041 cgctctggcg gcattgccga actgctcgcc cgggtcgcgg cgatggacgg gccgatcccg
     2101 ctggtgcaga tggccgatgc cgccggccat taccgggccg actggctggt cgaggaagtg
     2161 tcggtctcga tcaacaccac cgctgccggc ggcaatgcca gcctgatggc gctggtctaa
     2221 gtcccaatcc gggacctgtt cgcggctccg gccagagcga tgtggcgttt gtcacatcgc
     2281 gcagccacgc cgagtcctag catcgcttcc aaacaccaat tggaggcggg catggacatt
     2341 cttggcacca gcggcaacga catcctggat ggcactgaag gggacgatgt cttcaacgga
     2401 ttgggcggcg cgcaagacac gttttccggc cttggcggca gcgatacgtt cctgttctcg
     2461 acgcctgtct cgttcagcaa cgtctatgat ggcggcgcag gcaccgactg gttcgaactg
     2521 cgcactagcg tctttgccgc aatcgggcag gatggaaatc tcggttcggc ctatatcgtc
     2581 aatgcctcac tcactacctc ggtcgaaggc gtacgtttca ccgggactgt cggtgaaacc
     2641 cagcaacttg tcattgccag ctttttcggc ggcaatgccg ccgcggttcc gagcctgatc
     2701 gaaggcagcg ccgggcgcga tctcctggcc tttggggcct ttggcggaat gggaggtgct
     2761 gccgaactcg tcgtgcccac actttcagct tcgaactgga ccgggccagg cgcaatctac
     2821 cgcgatgagg gcgacgcggt gctgttctat attggcgatg caaacggtta tgtgctgcgc
     2881 gccaatgaag tcaatgccgc ggctggcatc gtccaggcgc tggtgggcgg agcgggtaac
     2941 gatacactga tcggatcggc aggcgcggac aagctggtca gcatcgtcgg agggaacgat
     3001 gcgctatatg gcaacggcgg caacgattct ctcgccgcga tcaaccaggc ccccaatggc
     3061 agcgccgcga tcaccaccct gacctttgcc ggatcaactt tcgacggggg cgatggcacc
     3121 gattttctga gcatcggcgg cagggtcaat tttcagggca ccttgaccag tatcgaaggg
     3181 atcaacctgt tgccgcagat cgcggttgcc aacccgtcgg ccccacgccg cgaagccgct
     3241 gaactcacta tttccagcgc gcagttggcc agcctgcctt cgaacctgca aatcgacgga
     3301 accggcaata tcgcggtcac catggtctct ggcgccgcgt tcaacgggtc gctctatcag
     3361 atgctggccg gatcggacgt caacttcacc atcaccggca ccagcggcaa cgagaccatc
     3421 accggcacca ccggcgacga tgtgatcctg ccggccggcg gggtcgacac gatccacgcc
     3481 gatgccggga acgacgaaat cgtgctgacc gcgccgatcg gtgcgggttc gatccttgat
     3541 ggcgggatcg gctacgacac gctcgtcctg cgtccgcaga gcacctatcc caacgccacg
     3601 gggggataca gcacccaata caacgcctat ttcccgaccc agatccaggg acttgaagct
     3661 gcccggtttg aaaccaatgc tggcgacaca
//
LOCUS       DASUDV010000488         5800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2026852,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000488 DASUDV010000000
VERSION     DASUDV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026852"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFV30_10580"
     CDS             <1..505
                     /locus_tag="VFV30_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX4848578.1"
                     /translation="DPENLQCNGRIGRFGFVLHGLWPESGQGKYPQWCSLTPRPTEAE
                     LKANLCMTPVPWLLEHEWAKHGSCMATTPAAYFKVSGILWNSLTLPDADKLSRQPGLT
                     AGDLRRAFLASNPGFRLDAIGLRLSNGGWLREIQLCYDRKFLPTACEPRQFGPKNDVP
                     LKIWRGL"
     gene            complement(638..1093)
                     /locus_tag="VFV30_10585"
     CDS             complement(638..1093)
                     /locus_tag="VFV30_10585"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX4848579.1"
                     /translation="MSRWPLPEPMNLALEQARRGESAGEVPIGAVVVKDGAVIAAAHN
                     APRRDHDPTAHAEVLAIRAAAAALGNERLDGCELWVTLEPCAMCAGAIAHARIARLYY
                     AAPDPKGGAVEHGARVFEHEQSLHRPEVYSGMGEDEAAGLLRAFFAAKR"
     gene            1424..1714
                     /gene="rpmB"
                     /locus_tag="VFV30_10590"
     CDS             1424..1714
                     /gene="rpmB"
                     /locus_tag="VFV30_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX4848580.1"
                     /translation="MSRICELTGKGRQVGHNVSHANNKTKRVFLPNLQNVTLMSEGLD
                     RSFKFRVSTQGLRSVEHVGGLDNWLLKTSDEKLSATALKVKRELKKKAAVAA"
     gene            complement(1788..2768)
                     /locus_tag="VFV30_10595"
     CDS             complement(1788..2768)
                     /locus_tag="VFV30_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX4848581.1"
                     /translation="MRRLILILLVALGLAPGTWLRSDVPPPSTAAVLTLNPLPLPAGC
                     CAGGAFTLTGAWELTSPNGSFGGYSALLADRPGRLLALSDRGYYLEFAEPGLGTGVPL
                     FGPVMADAARFKANRDIESAAIDLAGKRIWIAQEGRNGIARHRFDLARESFREPPEMA
                     VWSRNSGPEAMVRLADGRFVVLCECTPERFDDAGRHPALVYAGDPTGDRPGQAFTFAG
                     PADYRPTDMAQLPDGRVLVVVRRLTWPVPARFAIKLALADPREITPGGTWTAREVLDL
                     GGDWPVDNYEGLAIEQLPDGRILAWLISDENGAVTQRVLLLRLEIDPARL"
     gene            2880..3410
                     /locus_tag="VFV30_10600"
     CDS             2880..3410
                     /locus_tag="VFV30_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848582.1"
                     /translation="MRTLPCLAAAFAALTATPLLAKDKDAPPPQPVVFQAVLDCRTVA
                     DPTERLACFDRTAAALAQAAGANEVLVVDKDTARKTKRSLFGLSLPRVKIFGDRDDEE
                     IEQIESTIASTYTARDGQTVFVLPDGARWKQTDGRDTFPKAGQPIVIKKGALGSFFAR
                     VNGQTGVRVVRLPQTQ"
     gene            complement(3427..3738)
                     /locus_tag="VFV30_10605"
     CDS             complement(3427..3738)
                     /locus_tag="VFV30_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848583.1"
                     /translation="MIEALLLILAAESGAADAAVTEIQVAEPTPSTMTPKEIRAFNAK
                     LPRGHRYYIRCVARNETGSLVKQVRTCRTNEQWARAEETGNDNARETAEAMRGKASNS
                     N"
     gene            complement(3780..4085)
                     /locus_tag="VFV30_10610"
     CDS             complement(3780..4085)
                     /locus_tag="VFV30_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848584.1"
                     /translation="MSALLLVLAADAPVTATEALPLVEPNPKTMSQREIREFNTKLPR
                     DHPFYIKCVSSPEIGSLVKKSYSCRTNRQWHAADMQGNQNARDTYDEMTSKHWNTSN"
     gene            complement(4176..5369)
                     /locus_tag="VFV30_10615"
     CDS             complement(4176..5369)
                     /locus_tag="VFV30_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX4848585.1"
                     /translation="MTQPSPADPAPDLAVAQAANAEGLAAIRAGDAAAAVAAFTRATA
                     ADPLALPLWRNLAHAHRLAGNDPAEEAALERALTLDRLDFGTQLRMAQLLQRTGKETA
                     GLIAWNGVFQLAERQAGALPPAVEQELAAGRAYCEALQARLGAHADTLLAAGHDLWDE
                     TEQRRIKAFVDRTLGRRTVYVNECAGLHYPFLPADEFFDRRHFPWLEELEAHTAEIRE
                     ELLGILDDPGEAIRPYVRMEEGAPENKWSALDHSLDWGACFLWEHGEPNLPVHARCPR
                     TAAVLERLPLLRIPGRGPAAFFSILKPHSHIPPHTGVTNTRAIIHLPLIVPPGCGFRV
                     GGETREWIVGQAFAFDDTIEHEAWNTSDERRAVLIIDAWNPHLSEREREAITEYIRGS
                     DAALA"
     gene            5478..>5800
                     /locus_tag="VFV30_10620"
     CDS             5478..>5800
                     /locus_tag="VFV30_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848586.1"
                     /translation="MQPSPATLLADAEWLAHRYVESDDTFRFVRVPRADHSAIPFLTD
                     DLLGARTAGGDLPADACLALPGDAPLGLVFHSAFCGSTMLTRALDRPGMAMGLSEPVV
                     LNDVVG"
BASE COUNT          859 a         1989 c         2030 g          922 t
ORIGIN      
        1 ggatcccgag aacctgcaat gcaacgggcg gatcggccgg ttcggcttcg tgctccacgg
       61 gctctggccg gaaagcgggc agggcaagta cccgcagtgg tgcagcctga ccccgcgccc
      121 gaccgaggcc gagctcaagg ccaacctgtg catgactccg gtgccctggc tgctggagca
      181 cgaatgggcc aagcatggca gctgcatggc gaccactccg gcggcctatt tcaaggtctc
      241 gggaatcctg tggaactcgc tgaccctgcc cgatgccgac aaactgtcgc gccagccggg
      301 cctgaccgcc ggcgatctgc gccgcgcgtt cctggcgagc aatccgggct tccggctgga
      361 cgcgattggc ctgcgcctca gcaacggtgg ctggctgcgc gaaatccagc tctgctatga
      421 ccgcaagttc ctgccgaccg cctgcgagcc gcgccagttc ggcccgaaga acgacgtgcc
      481 gctgaagatc tggcgcgggc tttgaattcg gcttccctgg cagggaacca tcaccggccc
      541 actcccccgg cccaaccacc ccaagggtac catgtcacgg gtggttgggc cgggggagcg
      601 ggccggtgat gcatttcagc tctgctgaaa cagaagctca acgcttggct gcgaagaagg
      661 ccctcagcaa gccggccgcc tcgtcctcgc ccatgccgga atagacctcg ggccggtgca
      721 ggctctgttc gtgctcgaac acccgcgcgc cgtgctccac cgcgccgccc ttgggatcag
      781 gcgcggcgta atagagacgg gcaatccgcg cgtgggcgat cgcgccggcg cacatcgcgc
      841 aaggctccag cgtgacccac agctcgcacc cgtcgagccg ctcgttgccg agcgccgcgg
      901 ccgcggcgcg aatcgccagg acttcggcgt gggcggtggg atcgtgatcc ctgcgcggcg
      961 cgttgtgggc ggcagcaatc accgcgccgt ccttgaccac gaccgccccg atcggcactt
     1021 cccccgcgga ttcgccccgc cgcgcctgtt ccagcgccag attcatcggt tcgggcaggg
     1081 gccagcggct catgacactt gggctagagt ctttgttggg gtgcggcaag tttcttggct
     1141 gagtgcgcca tcggcatccg ccgatggctt gcctggccgg cccgctcccg tgttccagat
     1201 tcacgacaaa cgtccggggg acgttttcgc tggggcgccc cagccacccc aagggtacca
     1261 tgtcacgggt ggctgggccg ggggagcggg ccggccaggc aaagggaagc ccggaggggc
     1321 tgaccgcacg caaacgacag actcaccttg accccgcaag ggtgaatcgg tatgcgcgcg
     1381 ccttctcccg tcatgggcgt tcagtttttt agcgagttta gccatgtccc gtatttgcga
     1441 actgaccggc aagggtcgcc aggtcggcca caacgtcagc cacgccaaca acaagaccaa
     1501 gcgcgtcttc ctgcccaacc tgcagaacgt cacgctgatg tcggaaggcc tggaccgcag
     1561 cttcaagttc cgcgtttcga cgcagggcct gcgctcggtc gagcatgtcg gcggtctgga
     1621 caactggctg ctaaagacct cggacgaaaa gctttcggcc accgcgctga aggtgaagcg
     1681 cgagctcaag aagaaggctg ccgtggccgc ctaaggcccg cacttctgcc agaattcaaa
     1741 aggggcgtcc ggcgatccgg gcgccctttt tgcatgccct tccgcgctca gagccgcgcc
     1801 ggatcgattt ccagccgcag cagcagcacc cgctgggtga cggcgccgtt ctcgtcgctg
     1861 atcagccagg ccaggatccg cccgtcgggc agctgctcga tcgccaggcc ttcgtagttg
     1921 tcgaccggcc agtccccgcc cagatcgagc acctcgcgcg cggtccaggt gccacccggg
     1981 gtgatctcgc gcggatcggc cagcgccagc ttgatcgcga atcgggccgg caccggccag
     2041 gtcagccgcc gcaccaccac cagcacccgg ccatcgggca gctgcgccat gtcggtcggc
     2101 cggtaatcgg ccggcccggc gaaagtgaag gcctggcccg ggcggtcccc ggtcggatcg
     2161 ccggcataga ccagcgcggg atggcggccg gcatcgtcga accgttcggg cgtgcattcg
     2221 cacagcacca cgaaccgccc gtcggccagg cgcaccatcg cctcgggccc gctgttgcgc
     2281 gaccagaccg ccatttccgg cggctcgcga aagctctcac gggccagatc gaaccggtgg
     2341 cgggcgatcc cgttgcgccc ttcctgcgcg atccagatcc gcttgccggc cagatcgatc
     2401 gcggccgatt caatgtcacg gttggccttg aaccgcgcgg catcggccat caccggtccg
     2461 aacagcggaa cccccgtgcc caggcccggt tcggcaaatt cgaggtaata gccgcggtcg
     2521 ctgagcgcca gcagccggcc cggccggtcg gccagcagcg ccgaatagcc gccaaagctc
     2581 ccgttcgggc tggtcagctc ccaggccccg gtcagggtga aggccccgcc ggcgcagcac
     2641 cccgccggga gcggcaaggg attgagcgtc agcacggccg cggtactggg cggcggcaca
     2701 tcgctgcgca gccaggttcc gggcgcgagg cccagggcca caagcaggat caggatcagg
     2761 cgccgcatcg ccgctggctg tggcctgttg ccccaagcct tgcaagcgcc gcgattgacg
     2821 cggggcccgg cggcggggca ttgtgccgcc agcccagccc cgcccgagag gaccgatccg
     2881 tgcgcaccct gccctgcctt gccgctgcct ttgccgccct caccgccacg ccgctgctgg
     2941 ccaaggacaa ggacgcgccg ccgccccagc cggtggtgtt ccaggccgtg ctcgattgcc
     3001 gcaccgtggc cgatccgacc gagcggctgg cctgttttga ccgcaccgcc gcagccctgg
     3061 cccaggcggc cggggccaac gaggtgctgg tggtggacaa ggacaccgcc cgcaagacca
     3121 agcgcagcct gttcggcctg tcgctgccgc gggtgaagat cttcggcgac cgggatgacg
     3181 aggagatcga gcagatcgaa tcgacgatcg cttcgaccta taccgcgcgc gacgggcaga
     3241 cggtcttcgt cctgcccgat ggggcgcgct ggaagcagac cgacgggcgc gacacctttc
     3301 ccaaggccgg gcagccgatc gtgatcaaga agggcgcgct cggcagcttc tttgcccggg
     3361 tcaacgggca gaccggggtg cgcgtggtgc ggctgccgca gacgcaatag cgccggcagc
     3421 cccggctcag ttggagttgc tggctttccc gcgcatcgct tcggcggttt cgcgcgcgtt
     3481 gtcgttcccg gtttcctccg cgcgggccca ctgctcgttg gtccggcagg tgcggacctg
     3541 cttcaccagc gatccggttt cgttgcgggc cacgcagcgg atgtaatagc ggtggccgcg
     3601 cggcagcttg gcgttgaacg cccggatttc cttgggcgtc atggtcgacg gggtcggctc
     3661 ggccacctgg atttcggtca cggcggcatc ggcagcgccg gattcggcgg cgaggataag
     3721 gagcagggct tcgatcatgg ggatatcctg gcaaggccga gctgtccccg gcctgaacgt
     3781 cagttcgagg tgttccagtg cttgctggtc atctcgtcat aggtgtcgcg ggcgttctgg
     3841 ttcccctgca tgtcggcggc gtgccattgg cggttggtcc ggcaggagta ggacttcttc
     3901 accagcgagc cgatctcggg cgaggacacg cacttgatgt agaacggatg atcgcgcggc
     3961 agcttggtgt tgaactcgcg aatttctctc tgcgacatgg tcttgggatt gggttcgacc
     4021 agcggcaggg cttcggtggc ggtcacgggg gcatcggcgg ccaggaccag cagcagggcg
     4081 ctcaacatcg gttcacctcg tcggcggaga ccgccagcgg ggcggtcggg atcggcggag
     4141 actaaccgag cttgcccggg gcggcaagcg cgccgtcagg ccagcgccgc gtcggagccg
     4201 cggatgtatt cggtgatcgc ctcgcgctcg cgctcgctca ggtgcgggtt ccaggcatcg
     4261 atgataagca cggcgcggcg ttcgtcgctg gtgttccagg cctcgtgctc gatcgtgtcg
     4321 tcaaaggcga aggcctggcc cacgatccat tcgcgggttt ccccgccaac gcgaaaaccg
     4381 cagcccggcg gcacgatcag cggcaggtgg atgatcgcgc gggtgttggt cactccggtg
     4441 tgcgggggaa tgtggctgtg cggcttcagg atcgagaaga acgccgccgg cccgcgcccc
     4501 ggaatgcgca gcaggggcag ccgctccagc acggccgcgg ttcgggggca gcgggcgtgg
     4561 accggcaggt tgggctcgcc gtgctcccac aggaagcagg ccccccagtc gagcgagtgg
     4621 tccagcgcgc tccacttgtt ctccggcgcc ccctcttcca tccgcacata ggggcggatc
     4681 gcctcgccgg gatcgtccag aatgccgagc agctcctcgc ggatctccgc ggtgtgggct
     4741 tccagttcct ccagccaggg gaagtgccgg cggtcgaaga actcgtcggc ggggaggaac
     4801 ggatagtgca gcccggcgca ctcgttgaca tagaccgtgc ggcggccgag cgtgcggtcg
     4861 acaaaggcct tgatccggcg ctgctcggtt tcgtcccaca ggtcgtgccc ggcggccagc
     4921 aaggtgtcgg cgtgggcccc gagccgggcc tgcagggcct cgcaataggc gcgcccggcg
     4981 gcaagctcct gctccaccgc gggcggcaac gcgccggcct gccgttcggc cagctggaac
     5041 accccgttcc aggcgatcag cccggcggtt tccttgcccg tgcgctggag cagctgggcc
     5101 atgcgcagct gggtgccgaa atcgagccgg tccagcgtca gcgcgcgttc cagcgcggcc
     5161 tcttcggccg gatcgttccc ggccaggcgg tgggcgtggg ccaggttgcg ccacagcggc
     5221 agggccagag ggtcggcggc cgttgcgcgg gtgaaggcgg caacggcggc agcggcatcc
     5281 ccggcgcgga tcgcggccag cccttcggcg ttggcggcct gggcaaccgc gagatcgggg
     5341 gccggatcgg cggggctggg ctgggtcatg ggcttcctgc gctgcttggg cctgcgatag
     5401 gctgcgcccc ggctggccgc aagagggcgc acgccggggg tgttgcctcg gcctcggccg
     5461 ccgcctaagc agggaccatg cagcccagcc ccgccaccct gcttgccgac gcggaatggc
     5521 tggcgcaccg ctatgtcgaa agcgatgaca ccttccggtt cgtgcgggtt ccgcgggccg
     5581 atcattctgc catcccgttt ctgaccgacg acctgctggg cgcgcggacg gccggcggcg
     5641 atctgccggc cgatgcctgc ctggcgctgc ccggcgatgc cccgctaggg ctggtgttcc
     5701 attcggcctt ctgcggatcg accatgctga cccgggcgct cgaccggccg gggatggcga
     5761 tgggcctgtc cgagccggtg gtgctcaacg acgtggtcgg
//
LOCUS       DASUDV010000489         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2137240,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000489 DASUDV010000000
VERSION     DASUDV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137240"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFV30_10625"
     CDS             complement(<1..251)
                     /locus_tag="VFV30_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEX4848587.1"
                     /translation="MTRPATHILRLLRWGRTLARHGALRGIEKDPNTPPPVRRLTRIA
                     RAGTFQPKVPDYAGAFQAIGPAAIKLGQTLATRPDIVGE"
     gene            complement(253..984)
                     /locus_tag="VFV30_10630"
     CDS             complement(253..984)
                     /locus_tag="VFV30_10630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848588.1"
                     /translation="MSDKVSFGYEEVSPEEKTEKVGAVFSSVAKKYDVMNDAMSAGMH
                     RLWKDKFVRRVKPQPGEQILDMAGGTGDIAFRMAAHGATITVSDINQDMLDVGIERAM
                     ERGLDDLVWSRQNAETLTFPDRFFDAYTIAFGIRNVTRIDHALAEAHRVLKHGGRFFC
                     LEFSTTEWPGFKEVYDAYSHHLVPKIGEAIAGDADSYRYLVESIRRFPTMPEFEGMIR
                     QAGFVNTRVEPILGGLVAIHSGWKI"
     gene            complement(1048..1572)
                     /locus_tag="VFV30_10635"
     CDS             complement(1048..1572)
                     /locus_tag="VFV30_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX4848589.1"
                     /translation="MSASLYHTHVPGFIAMLRNMQAWLDKGAAHSGEAALMEARLAED
                     MLPLPRQIQIASDAAKGAAARLTGTEPPAMPDTETSLAELKERCAKTIAYLESVDPAA
                     FDAGAAREVVITFPNGAGIKLDGQTFLTGFATPNFYFHATTTYAILRVLGVEIGKQDY
                     LAHLAPFMFGPPQG"
     gene            1681..2496
                     /gene="mutM"
                     /locus_tag="VFV30_10640"
     CDS             1681..2496
                     /gene="mutM"
                     /locus_tag="VFV30_10640"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX4848590.1"
                     /translation="MPELPEVETTVRGLARYLDGARLARVEPRRADLRRPFPADLVQV
                     LTGATVTGLSRRAKYGLIHTDRDQTMVFHLGMSGRWRIEPEELGKHDHLVLETGGGHV
                     LALFDPRRFGSVDLVPTPQLGAWPAFAALGPEPLGEELTPAHLKTALAGRSAAIKLML
                     LDQRIVAGLGNIYVCEALFRAGIDPRKAAGKVTGPALTRLVPQIKTVIAEAIEAGGST
                     LRDYAQPDGELGYFSKQFDVYDRHGQDCTRCGAPVQRLAQGGRSTWYCGKCQK"
BASE COUNT          393 a          840 c          905 g          442 t
ORIGIN      
        1 tcctcgccga caatgtcggg gcgggtggcc agggtctggc ccagcttgat cgcggccggg
       61 ccgatcgcct gaaaggcccc ggcatagtcc ggcaccttgg gctggaaggt gccagcccgc
      121 gcaatccggg tcagccggcg gaccgggggc ggggtgttgg gatccttctc aatcccgcgc
      181 agcgcgccgt gccgggcgag cgtgcgcccc cagcgcagca ggcgcaggat atgcgtcgcc
      241 gggcgggtca cttcagatct tccaccccga atggatcgcg accagtccgc ccaggatcgg
      301 ttcgacccgg gtgttcacga agccggcctg gcggatcatg ccttcgaatt cgggcatggt
      361 cggaaaacgg cggatcgatt cgacgagata gcggtagctg tcggcatcgc cggcaatggc
      421 ctcgccgatc ttcggcacca ggtggtgcga ataggcatcg tagacttcct tgaagcccgg
      481 ccattcggtg gtcgagaatt ccaggcagaa gaaccgcccg ccgtgtttca gcacgcggtg
      541 cgcttcggcc agggcgtgat cgatgcgggt gacgttgcgg atcccgaagg cgatcgtgta
      601 ggcatcgaag aagcggtccg ggaacgtcag cgtctcggcg ttctggcgcg accagaccag
      661 gtcatccagc ccgcgctcca tggcccgttc gatgccgacg tccagcatgt cctggttgat
      721 gtcggacacg gtgatcgtgg cgccgtgcgc ggccatgcgg aaggcgatgt cgccggtgcc
      781 gccggccatg tcgaggatct gctcgcccgg ctggggtttc acccggcgga cgaacttgtc
      841 cttccacagc cggtgcatgc ccgccgacat cgcgtcgttc atgacatcgt acttcttggc
      901 gacggaggaa aacaccgcgc cgaccttctc ggtcttctcc tcgggagaga cctcttcgta
      961 accgaaggaa accttgtcgc tcatggcccg cgccttagcg gcaaggcggg cgcggtgcca
     1021 gtgaaagcgt gtgcagttcc cgtctgttca cccttgcggc gggccgaaca tgaacggcgc
     1081 gaggtgggcc aggtaatcct gcttgccgat ttccacgccc agcacccgca ggatcgcgta
     1141 agtggtggtg gcgtggaagt agaaattggg ggtggcgaag ccggtcagga aggtctgccc
     1201 gtccagcttg atccccgccc cgttggggaa ggtgatcacc acctcgcgcg ccgcaccggc
     1261 atcgaacgcc gccggatcga ccgattccag ataggcgatg gtcttggcgc agcgttcctt
     1321 cagttcggcc aggctggtct cggtgtccgg catggccggc ggctcggtcc cggtcagccg
     1381 cgcggcggcg cccttggccg cgtccgaggc gatctggatc tggcggggca ggggcagcat
     1441 gtcttcggcc agccgcgctt ccatcagcgc cgcctcgccg ctgtgggccg cgcccttgtc
     1501 cagccaagcc tgcatgttgc gcagcatggc gatgaagccg gggacgtggg tgtgatagag
     1561 cgaagcggac atggcggatt ctcccggtag gtttgattgg ctgctggtta ggggcaaagg
     1621 gcgccccgtt tcaatacgaa accgattgtc gcagggcctt gcagtgccta accagcggcc
     1681 atgccagagc tacccgaagt cgaaaccacc gttcgcggcc ttgcccgcta tctcgatggc
     1741 gcgcgcctgg cgcgggtcga gccgcgccgc gccgatctgc gccggccctt tccggccgat
     1801 ctggtccagg tcctgaccgg ggccacggtg accggcctgt cgcgccgggc caaatacggc
     1861 ctgatccaca ccgatcggga tcagaccatg gtgttccacc tcggcatgtc cggacgctgg
     1921 cggatcgagc ctgaggagct gggcaagcac gaccacctgg tgctggaaac gggcggcggg
     1981 cacgttctgg cgctgttcga tccgcgccgg ttcggctcgg tcgatctggt gccgacgccg
     2041 cagctgggcg cctggccggc ctttgccgcg ctggggccgg agccgctggg cgaggagctc
     2101 acccccgcgc acctcaagac cgcgctggcc gggcgcagcg ccgcgatcaa gctgatgctg
     2161 ctcgatcagc ggatcgtggc cgggctgggc aacatctatg tctgcgaagc gctgttccgc
     2221 gccgggatcg atccgcgcaa ggcggccggc aaggtaaccg gcccggcgct gacccggctg
     2281 gtcccccaga tcaagacggt gatcgccgag gcaatcgagg cgggcggatc gaccctgcgc
     2341 gactatgccc agccggatgg cgagcttggc tacttctcca agcagttcga cgtctatgat
     2401 cgccacgggc aggactgcac ccgctgcggc gcgccggttc agcgcctggc ccagggcggg
     2461 cgcagtacgt ggtattgcgg gaagtgccag aagtgagggg tgggagtctg ttgtccgcct
     2521 gacggcggtg cggacagacg tccgtctgtc ctggcatcac cggcccgctc ccccggccca
//
LOCUS       DASUDV010000490         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_833647,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000490 DASUDV010000000
VERSION     DASUDV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833647"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFV30_10645"
     CDS             <1..780
                     /locus_tag="VFV30_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEX4848591.1"
                     /translation="HALQKSRIEKIVILGRRGPHQIAMTPKELGELAHLARACPRVDP
                     ADLPDEAEDLFLEPGQRKSVGHLRGFAAIPEEFRCDKPIEIEFDFFASPRAVLGDGKV
                     EGVEVERTVLDDQGRATSTGETYRIDCGLVITCIGYKSSPIPGVPFEESAGRFANDEG
                     RILPGLYCVGWARRGPSGTIGTNRPDGFAVVERIAEDLEEGAGKRGRAGFDELAAARK
                     LDIVTFRDWKKIEAEEEARARSGAPREKFVDVDAMIKARGD"
     gene            843..1817
                     /locus_tag="VFV30_10650"
     CDS             843..1817
                     /locus_tag="VFV30_10650"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4848592.1"
                     /translation="MESSLPVRRFDMLDAYRGIAALAVLFYHAGAQAPLLAPRGYLGA
                     DLFFILSGFVLAHAYGARLAGGLGLSGFIRLRLIRIYPMFLMGAVLGALLFHGNPLML
                     LMLPTPSADGLLFHANAPLWSVVFELLVSLGYCLLAPRLGLRGLLAIAGVTGLVLARG
                     ILTHDGADLGAFAATAGLGLVRTAFCFTIGVLLHRAYLARHRVPATGPLGLALPVVLV
                     LVLMIDPAGRAWWDIATIGAIFPALIWLGACTALPRPALGERLGGMSYPLYCIHAPIV
                     AMCHGSARLMGLACLALVALAQVLDRRFDRPLQRLARREPSPGRIASA"
BASE COUNT          281 a          660 c          684 g          386 t
ORIGIN      
        1 cacgcgctcc agaaatcgcg gatcgagaag atcgtgatcc tgggccggcg cgggccgcac
       61 cagatcgcga tgacccccaa ggagctgggc gaactggcgc atctggcacg ggcctgcccg
      121 cgggtcgatc cggccgatct gcccgacgaa gccgaagacc tgttccttga acccggccag
      181 cgcaagtcgg tcggccatct gcgcgggttt gccgcaatcc ccgaggaatt ccgctgcgac
      241 aagccgatcg agatcgagtt cgatttcttc gccagccccc gcgcggtgct tggcgatggc
      301 aaggtcgaag gggtcgaggt tgagcgtacc gtgctcgatg atcagggccg cgccacgtcc
      361 accggcgaaa cctaccggat cgattgcggg ctggtgatca cctgcatcgg ctacaagagc
      421 tcgccgatcc cgggggttcc gtttgaggaa agcgccgggc gcttcgccaa cgacgaaggg
      481 cggatcctgc ccgggctcta ctgcgtcggc tgggcgcggc gcgggccatc gggcacgatc
      541 ggcaccaacc gccccgatgg ctttgcggtg gtcgaacgga tcgccgagga cctggaggaa
      601 ggcgcgggca agcgtggccg cgccgggttc gacgagctgg ccgccgcgcg caagctcgac
      661 atcgtcacct tccgcgactg gaaaaagatc gaagccgaag aggaagcccg cgcccgctcc
      721 ggcgccccgc gtgaaaagtt cgtcgatgtc gatgcgatga tcaaggcgcg cggcgactga
      781 acccgtcagc aagccccgac aatcggggtt tgttaatcga aatgggctag gaccctggcc
      841 ccatggaaag cagccttccc gtgcggcggt tcgatatgct ggatgcctat cggggcatcg
      901 cggcgctcgc ggtgctgttc tatcatgccg gagcccaggc tccgttgctg gcgccgcgcg
      961 gctatctcgg cgcggacctg ttcttcatcc tttcgggatt cgtgctggcc catgcctatg
     1021 gcgcgcggtt ggcgggcgga ctggggctgt ccgggttcat ccggctgcgg ctgatccgga
     1081 tctatccgat gttcctcatg ggcgcggtgc tgggggccct gctgttccac ggcaacccgc
     1141 tgatgctgct gatgctgccc acgccctcag ccgatggcct gctgttccat gccaatgctc
     1201 cgctgtggtc ggtcgtgttc gaactgctgg tcagcctggg ctactgcctg ctcgcaccca
     1261 gattggggct gcgcgggctg ctggcaatcg ccggcgtgac cgggctggtt ctggcccgcg
     1321 gaattctcac ccacgatggc gccgatctcg gagcctttgc ggcaaccgcc gggcttggcc
     1381 tggtccgcac cgcgttttgt ttcaccatcg gggttttgct gcatcgcgct tacctggccc
     1441 ggcatcgcgt gcctgccacc ggcccgctgg gcttggcctt gccggttgtg ctggttcttg
     1501 tgctgatgat cgatccggca gggcgggcgt ggtgggacat tgccaccatt ggcgcgatct
     1561 ttcccgccct gatctggctt ggcgcctgca ccgcgctgcc ccggcccgcg ctgggcgaaa
     1621 ggctcggcgg gatgtcctat ccgctctatt gcatccatgc cccgatcgtg gcgatgtgcc
     1681 acggctcggc ccggttgatg ggccttgcct gtctggccct ggtagccctg gcccaggtgc
     1741 tcgatcgccg gttcgaccgg ccgttgcaac ggttggcccg gcgtgaacct tcgcctggcc
     1801 ggattgcctc ggcatagggc ttgccccttc tcccaagctg tgtcttaatc gcccggttaa
     1861 gcgcgccctg accagagtcg gcgcgatccg ggagagtgag ccatgcccag ctacgatctg
     1921 atcattcgag gcggcaccat cgtcgatggc accggcgccg cgcggtttac cggcgatgtt
     1981 gcggtgaagg acgggctgat cgccgcggtc g
//
LOCUS       DASUDV010000491         8085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1651445,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000491 DASUDV010000000
VERSION     DASUDV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651445"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1408
                     /locus_tag="VFV30_10655"
     CDS             170..1408
                     /locus_tag="VFV30_10655"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX4848593.1"
                     /translation="MKETAQTAAEQLTVDDVRGAAARIKGAVVRTAMDYSRTLSQITG
                     ADIWLKFENLQFTAAYKERGALNALLHLTEEQRSRGVIAASAGNHAQGLSYHGSRLGI
                     PVTIVMPRSTPTVKVMQTEAVGGTVMLEGETFDEAYAHARSMERQLGLTFVHPFDDPL
                     IAAGQGTVALEMLEDVPDLDMLVIPVGGGGLASGMGVAARAVKPDIALIGVEAELFPS
                     MYNRLRHNELPCGGDTLAEGIAVKEPGVFTARTLRGLLDDIVLVDEGSLERAVSLLLQ
                     IEKTVAEGAGAAGLAAVLAHPARFKGRKIGIPITGGNIDTRLLANVLLRDLARSGRLA
                     RLRLTLQDRPGALFKVMREFDRHNVNIIEIYHQRIFTHLPAKGLITDIECEARDKEQL
                     DALVETLREKGYAVTAVELN"
     gene            1575..2429
                     /locus_tag="VFV30_10660"
     CDS             1575..2429
                     /locus_tag="VFV30_10660"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX4848594.1"
                     /translation="MTAPVRFPRFFVTSPAPCPYLPGRTERKVFTELKGPHAAELNDA
                     LGRIGFRRSQTVAYRPSCADCQACVSVRVVAGEFRASASQKKNLRHNGDLVATVCRPW
                     STAEQFDLLQRYLGQRHPGGGMTTMDEVDFADMVEHTPVDSWVVEYREPTVDGSPGRL
                     VGACLTDRQGDGLSMIYSFYDPDHPSRTGLGNYIILDHIQRARAEGLPFVYLGYWVDG
                     SARMQYKVRYRPIEQLGRQGWVRMSDSEQDALIAKAALLRRETAQPLDGSTKDGAHGG
                     QVQYRLDV"
     gene            complement(2426..3076)
                     /locus_tag="VFV30_10665"
     CDS             complement(2426..3076)
                     /locus_tag="VFV30_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX4848595.1"
                     /translation="MKQRLSAEALGSFFLFATVVGSGIMAERLAQGNDAVALLGNTVA
                     TAAMLFVLIAMLGPISGAHFNPAVSLVMRLRGDCCWKGLAATVAVQVLAGILGVWAVH
                     AMFDLPVLQVSQKLRDGPGQWLGEFIATFGLVITILLTVRHRPAAVPASVALYITAAY
                     WFTSSTSFANPAITIARSLSDSFAGIAPASVLPFLAAQLAGALAAHGFEKSVLKPD"
     gene            complement(3073..3576)
                     /locus_tag="VFV30_10670"
     CDS             complement(3073..3576)
                     /locus_tag="VFV30_10670"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX4848596.1"
                     /translation="MVQVRSVLVLCTGNSARSILGEALFATLGAGRLTAYSAGSKPKG
                     VPHPGALRQLAAQGIDTAPFRSKSWLEFTGPDAPEIDLAITVCGNAAGEACPVFPGTP
                     LRAHWGLPDPADVTGGEAAEDAAFAETWRLLEMRIQAMLALPLDTMDRAEAEAALAAI
                     GQMEGAA"
     gene            complement(3580..4056)
                     /locus_tag="VFV30_10675"
     CDS             complement(3580..4056)
                     /locus_tag="VFV30_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX4848597.1"
                     /translation="MKRFHVHVGVTDLDQSIAFYTGLFGQAPSVTKADYAKWMLEDPR
                     VNFAISQKCGAVKGVEHLGLQVEDQAELAEVYGALAAAGRPVLEEGATTCCYAQSEKS
                     WIADPDGVVWEAFLTSGESTVYGSGPELEQLASANAAAGACCAPQAAPAATTGGCC"
     gene            complement(4053..4379)
                     /locus_tag="VFV30_10680"
     CDS             complement(4053..4379)
                     /locus_tag="VFV30_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4848598.1"
                     /translation="MQPDSVIRSLGALAQEHRLAAFRLLVQAGPDGMAAGALAEALGI
                     PASSLSFHLAQLSNAGLVSQRRVSRSIIYAADYAAMNGLLSYLTENCCAGGSCAPAQT
                     AERMAS"
     gene            complement(4448..5902)
                     /locus_tag="VFV30_10685"
     CDS             complement(4448..5902)
                     /locus_tag="VFV30_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4848599.1"
                     /translation="MINRRHFLSAAALSGAALAAPAVVRAKRTDSGDIRLLGEILRTL
                     HPGLHRYLTPYRFEAALDRLDSRWQGGATLETRFAELTRFLALIRCGHSYPSFYNQKR
                     AVADRLFTRKDRLPFAFRWIGGQMVVTQNQTDGAALPVGSVIKAIGGVPTEAILARLT
                     PYVRADGGNDGKRRALLSASGGDTIETFDVLYGLVYGPPRGGVFNVAYRAPGAASDAQ
                     GDFAPIDLAARQRFIRAIDPRSNQPLWQWDMGSDGIARLTMPGWAVYNSKWDWQGWLN
                     DRLDSLKGARGLIVDLRENEGGNDCGDLILARLAGQDIVRPQVDRLVRYRRVPSALNP
                     YLDTWDDSFRDWGDAAKPFNARFLRLDRADEGVIAAKGPRLAVPMAVLTSPQNSSATF
                     QFASLVRVTGLGTLIGETTGGNRRGINGGAFFFARLPESGIEFDVPLIGYFPQGPQPN
                     AGLDPDIRVAETAEDIAAGRDRAMEAARAHLLRG"
     gene            complement(5961..7067)
                     /locus_tag="VFV30_10690"
     CDS             complement(5961..7067)
                     /locus_tag="VFV30_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4848600.1"
                     /translation="MLDGLSFGWRTAVLTVAVVQLLILAAALTRTIANRAANRTLAAL
                     LVVLTGQVMPWLIGFAGFYDKWMWLTFAPFQITLAVAPLGWLYAVALTEGGWPARGWR
                     HLIPAAVQFAYLAGCFVLLPLPAKLAWGEASGAATSLLTGAALLAQMAWYLPATARQL
                     RRYRTALGEHVADEQRYAGRWLAAALAALGTLFVVWTVYLIWDLVAPLGYTGLMGLYL
                     AMAACALFLGIEGWRHAALPFPRLGDLQLAPAPEPRDWAALGREWAERVRTEGWARDP
                     DLTLARLARRLGTNTGYLSRAINQGEGTNFASFVARLRAEAVAQRLRAGDPADLLTIA
                     LEEGFGSKASFNRAFTAALGEVPSAYRRRVSKGE"
     gene            complement(7179..>8085)
                     /locus_tag="VFV30_10695"
     CDS             complement(7179..>8085)
                     /locus_tag="VFV30_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4848601.1"
                     /translation="ECPTNAAIFAEILDAAGVPPGVFYLVQGDGPTTGNAISSHPDVE
                     MVSFTGSTRAGIQVAIAAAPTVKRVHQELGGKSPNLVLPDADLATHLPAVAMGPLVNT
                     GQSCISPTKVLVAKDREAEVVGFYKAMYAATPVGDPMAEGNHLGPVVNKAQFEKVRSL
                     IQSAIDEGAKLEAGGPDLPPNVNRGYYIQPTVFSGVTPDMRIAQEEIFGPVATIMTYD
                     SLEQGIELANDTEYGLSAAITGDPAKAAEVAGQLRAGMVAINNWGPTPGAPFGGYKQS
                     GNGREGGLAGLKDFMEMKAISGLPA"
BASE COUNT         1311 a         2752 c         2797 g         1225 t
ORIGIN      
        1 atgtgccgcg cgatcgcgcc gcccatcacg cccaggccca ggaaggagac attgagggga
       61 gtggtcatgg cagcggtgtg tagcgccttt tccggcaaag caaagctgcc gcgcgcaaaa
      121 gcaaatcgag gtttccccgc cacccgcttt gcgttacggc ccccgcatta tgaaagaaac
      181 cgcccaaacc gctgctgaac agctgactgt agacgacgtc cgtggcgctg ccgcccggat
      241 caagggtgcc gttgtgcgca ccgcgatgga ttacagccgg accctgtcgc agattaccgg
      301 ggcggacatc tggctgaagt ttgagaacct gcagttcacc gcggcctaca aggagcgcgg
      361 ggcgctgaac gcgctgctgc acctgaccga ggaacagcgc agccgcggcg tgatcgcggc
      421 ctcggccggc aaccacgccc agggcctgtc ctatcacggc agccggctgg gcattccggt
      481 caccatcgtc atgccgcgct ccaccccgac ggtgaaggtg atgcagaccg aagcggtcgg
      541 cggcaccgtg atgctggagg gcgagacctt tgacgaggcc tatgcccacg cccgcagcat
      601 ggagcgccaa ctggggctga ccttcgtcca cccgttcgac gatccgctga tcgccgccgg
      661 gcagggcacg gtcgcgctgg aaatgctgga agacgtcccg gatctcgaca tgctggtgat
      721 cccggttggc gggggcgggc tggcctcggg catgggcgtc gccgcgcgcg cggtgaagcc
      781 ggacatcgcc ctgatcgggg tggaggcgga gctgttcccc tcgatgtaca accgcctgcg
      841 ccacaacgaa ctgccctgcg gcggggacac cctggcagaa gggatcgcgg tgaaggagcc
      901 gggcgtgttc accgcgcgga ccctgcgcgg cctgctcgac gatatcgtgc tggtggacga
      961 aggttcgctc gaacgggcgg tttcgctgct gctgcagatc gaaaagaccg tggccgaagg
     1021 cgcgggcgcg gcgggcctcg ctgcggtgct ggcccatccg gcccggttca agggccgcaa
     1081 gatcggcatc ccgatcaccg gcggcaacat cgacactcgc ctgctcgcca acgtgctgct
     1141 gcgcgatctg gcccgctcgg gccggctggc acggctgcgg ctgaccctgc aggaccgccc
     1201 cggtgcgctg ttcaaggtga tgcgcgaatt tgaccggcac aacgtcaaca tcatcgagat
     1261 ctaccaccag cggatcttca cccatctgcc ggccaagggc ctgatcaccg acatcgaatg
     1321 cgaagcccgc gacaaggagc agctcgacgc cctggtcgag acgctgcgcg aaaagggcta
     1381 cgccgtcacc gcggtggaac ttaactagcg cgtccggacc tgtcccgttt tgcgcgggac
     1441 ggtccgcgcc gctgcgccgg gctgtgcata ataacgatcg cttaaccgtg tttcgtggtt
     1501 aaagttcttt caacgccgcc ccggcccatg catagtgacg gccttcacca agccatcagg
     1561 atggtcctcg cagcgtgacg gcccccgttc gatttccccg gtttttcgtc acgagccccg
     1621 cgccgtgtcc ctatttgccg gggcgcacgg aacgcaaggt gttcaccgag ctgaagggcc
     1681 cgcacgccgc ggaactgaac gacgcgctgg gccggatcgg cttccgccgc agccagaccg
     1741 tggcctatcg ccccagctgc gccgattgcc aggcctgcgt ttcggtgcgg gtggtcgcgg
     1801 gcgaattccg cgcttcggcc agccagaaga agaacctgcg ccacaacggc gatctggtcg
     1861 ccaccgtctg ccgcccttgg tcgacggctg aacagttcga cctgctgcag cgctatcttg
     1921 gccagcgcca tcccggcggc ggcatgacca ccatggacga ggtcgatttc gccgacatgg
     1981 tggaacacac gccggtggac agctgggtgg tggaataccg cgaaccgacc gtggacggat
     2041 cgcccgggcg gctggtcggc gcctgcctga ccgaccggca gggcgatggg ctgtcgatga
     2101 tctattcgtt ctacgatccc gatcatccca gccgcacggg cctgggcaac tacatcatcc
     2161 tcgatcacat ccagcgcgcc cgggccgaag ggctgccgtt cgtctacctc ggctattggg
     2221 tcgatggctc ggcgcggatg cagtacaagg tccgctaccg cccgatcgag cagctcggcc
     2281 ggcagggctg ggtgcggatg agcgattccg aacaggacgc gctgatcgcc aaggccgcgc
     2341 tgctgcgccg cgaaaccgcc cagccgctcg acggcagcac caaggacggc gcccacggcg
     2401 gccaggtgca gtaccggctc gacgtttagt ccggtttcag caccgacttc tcaaacccgt
     2461 gcgcggccag cgcgcccgcc agctgcgcgg ccaggaacgg cagcacgctg gcaggagcga
     2521 tcccggcaaa gctgtcgctg aggctgcggg cgatggtgat cgccgggttg gcaaagctgg
     2581 tcgatgacgt gaaccagtaa gccgcggtga tgtagagcgc gacgctggcc ggaacggcgg
     2641 cggggcggtg acgcacggtc agcaggatcg tgatgaccag cccgaaagtg gcgatgaatt
     2701 cgcccagcca ttggcccggc ccgtcgcgca atttttggga gacctgcagc accggcagat
     2761 cgaacatcgc gtggacagcc cagaccccca ggatcccggc cagcacctgc accgccacgg
     2821 tcgcggccag gcctttccag cagcaatcgc cgcgcagccg catcaccagg ctcaccgccg
     2881 ggttgaaatg cgcgcccgag attgggccga gcatggcgat cagcacgaac agcatggcgg
     2941 ccgttgcgac cgtgttgccc agcagcgcca cggcgtcgtt gccctgcgcc agccgctcgg
     3001 ccatgatccc ggagccgacc acggtggcga agaggaagaa actgccgagc gcctcggccg
     3061 acagccgttg cttcatgccg cgccttccat ctgcccgatc gcggccagcg ccgcttccgc
     3121 ttcggcgcgg tccattgtgt ccagcggcag ggccagcatg gcctgaattc gcatttccag
     3181 cagccgccag gtttcggcaa aggcggcgtc ctcggccgcc tcgccgccgg tcacgtcggc
     3241 cggatcgggc aggccccagt gggcgcggag gggcgtgccg ggaaagaccg ggcaggcttc
     3301 gcccgccgcg ttaccgcaga cggtgatcgc gaggtcaatt tctggcgcgt ccgggccggt
     3361 gaattccagc cagctcttgg agcggaacgg ggcggtatcg atcccctgtg cggccagctg
     3421 ccgcagcgcg ccggggtggg gcacgccctt gggcttgctg ccggcgctgt aagcggtcag
     3481 ccgcccggca ccaagcgtgg cgaagagcgc ttcacccagg atcgagcggg cggaattgcc
     3541 ggtgcacagc accagcaccg aacggacttg gaccatggct cagcagcagc cgccagtcgt
     3601 cgcggccggc gcggcctgcg gggcgcagca ggcaccggcg gcggcattgg ccgaggcgag
     3661 ctgctccagt tccggcccgc tgccatagac agtcgattcc ccgctggtca ggaaggcttc
     3721 ccagacgacg ccgtccggat cggcgatcca gctcttctcg gactgggcat agcagcaggt
     3781 ggtcgcgcct tcttccagca ccgggcggcc cgccgcggcc agcgcgccat agacttcggc
     3841 cagttcggcc tggtcttcca cctgcaggcc cagatgctcc acgcccttca ccgcgccgca
     3901 cttttgcgag atcgcgaagt tgacccgcgg gtcctccagc atccacttgg catagtcggc
     3961 cttggtgacg ctgggcgcct gcccgaacag gccggtgtaa aaggcgatcg actgatcgag
     4021 gtccgtcacc ccgacatgga catggaaacg cttcatgacg ccatcctttc agcagtctgc
     4081 gcgggcgcac aggagccccc ggcgcagcag ttttcggtga ggtaagagag caggccgttc
     4141 atcgcggcat agtcggccgc gtagatgatc gaacggctga cccggcgctg gctgaccagc
     4201 ccggcgttgc tcagctgggc gaggtggaac gagagcgacg aggctggaat gcccagcgcc
     4261 tcggccaggg cgcccgccgc catgccgtcc ggcccggcct ggaccagcag gcggaacgcg
     4321 gccagccggt gctcctgcgc cagggcgcca agcgagcgga tgacggaatc gggctgcatc
     4381 gctttccttc catgattcga tgattatcga aatatggaag gaaaggcgtg gtgtcaagcg
     4441 gtggcggtca gccgcggagc aggtgggcgc gggccgcctc catcgcccgg tcgcggcccg
     4501 cggcgatgtc ctccgcggtt tcggcgacgc ggatgtcggg atcgagcccg gcattcggct
     4561 gcggcccttg gggaaagtag ccgatcagcg gcacatcgaa ttcgatcccg ctttccggca
     4621 ggcgcgcaaa gaaaaaggcc ccgccgttga tcccccggcg gttgccgccg gtcgtctcgc
     4681 caatcagcgt gcccagcccg gtgacccgga ccaggctagc gaactggaac gtcgcgctgg
     4741 aattctgcgg actggtcagg accgccatcg gcacggccag gcgcgggccc ttggcggcga
     4801 tcacgccttc gtccgcgcgg tcgagccgca ggaagcgggc gttgaagggc ttggccgcat
     4861 cgccccaatc gcgaaagctg tcgtcccagg tgtcgagata ggggttcagc gcggatggaa
     4921 cccgtcggta gcggaccagc cggtccacct ggggccggac aatgtcctgc ccggccaggc
     4981 gggccaggat caggtccccg caatcgttgc cgccctcgtt ctcgcgcagg tcgacgatca
     5041 ggccgcgtgc gcccttcagg ctgtcgagcc ggtcgttcag ccagccctgc cagtcccact
     5101 tgctgttgta gaccgcccag cctggcatgg tcagccgggc aatcccgtca ctgcccatgt
     5161 cccactgcca cagcggctga ttgctgcgcg ggtcgatcgc ccggatgaag cgctggcgcg
     5221 cggcaagatc gatcggcgcg aaatcgccct gggcatcgct ggccgcgccg ggtgcgcggt
     5281 aggccacgtt gaacaccccg ccgcgcggcg ggccatagac cagcccgtag agcacgtcga
     5341 aggtctcgat cgtgtcgccc ccgctggcgg aaagcagcgc ccggcgcttg ccgtcgttgc
     5401 cgccatcggc gcggacatag ggcgtcagcc gggccaggat cgcctcggtc ggcacgccgc
     5461 cgatcgcctt gatcacgctg cccaccggca gggcggcgcc gtcggtctgg ttctgggtga
     5521 ccaccatctg tccgccgatc cagcgaaagg cgaagggcag gcggtccttg cgggtgaaca
     5581 gccggtccgc caccgcgcgc ttctggttgt agaagctggg ataggaatgg ccgcagcgga
     5641 tcagggccag gaaccgggtc agctcggcaa agcgggtttc cagcgtggcg ccgccttgcc
     5701 agcggctgtc cagccggtcg agcgcggctt cgaaccggta gggggtgaga tagcggtgca
     5761 ggcccgggtg cagcgtgcgc aggatctcgc ccaagaggcg gatgtcgccg gaatcggtgc
     5821 gcttggcccg gaccacggca ggggcggcaa gggccgcgcc cgaaagcgcg gcggcggaga
     5881 ggaagtggcg gcggttgatc atgggccgtg ctgtctccga tccggcgcgg cctcgcgcct
     5941 caaattccgc aacgggcggc ttattcacct tttgagacgc ggcggcggta ggccgatggc
     6001 acttcgccca gcgcggcggt gaaggcgcgg ttgaagctcg ccttcgatcc aaagccttcc
     6061 tccagcgcga tggtgagcag atcggccgga tcgccggcgc gcagccgctg cgccaccgcc
     6121 tcggcccgca gccgggcgac gaagctggcg aaattggtgc cttcgccctg gttgatcgcg
     6181 cggctgagat agccggtgtt ggtgccgagc cgccgggcca gccgggccag ggtgagatcc
     6241 ggatcgcggg cccagccttc ggtccgcacc cgctccgccc attcacggcc cagtgcggcc
     6301 cagtcgcgcg gttcgggcgc gggggccagc tgcaggtcgc ccaggcgcgg aaagggcagg
     6361 gcggcgtggc gccagccctc gatccccagg aacagcgcgc aggccgccat cgctaagtag
     6421 aggcccatca gcccggtgta gcccagcggc gcgaccaggt cccagatcag gtagactgtc
     6481 cagaccacga acagcgtgcc cagcgcggcc agcgcggcgg ccagccagcg cccggcatag
     6541 cgctgctcgt ccgccacgtg ctcgcccagc gcggtgcggt aacggcgcaa ttgccgcgcc
     6601 gtggccggca gataccaggc catctgcgcc agcagcgccg cccccgtcag cagcgaggtg
     6661 gccgcgcccg aagcctcgcc ccaggccagc ttggccggca gcggcagcag cacgaaacag
     6721 cccgccagat aggcgaattg caccgccgcc gggatcaggt gccgccagcc ccgcgcgggc
     6781 cagcccccct cggtcagcgc cacggcatag agccagccca gcggtgccac cgccagggtg
     6841 atctggaacg gcgcgaaggt cagccacatc cacttgtcgt agaacccggc aaagccgatc
     6901 agccagggca tgacctgccc ggtcagcacc accagcagcg ccgccagcgt gcggttggcg
     6961 gcgcggttgg caatcgtgcg ggtgagcgcc gcggcgagga tcagcaattg caccaccgca
     7021 acggtcagga ccgcagtgcg ccatccgaaa ctcaggccat ccagcatggt cgaagcgttg
     7081 cagatcgggc cggacacggc aagcgtcccg accacaaaag caaaccccct ccccgcgggt
     7141 gcgggaaggg ggttggcgtt cgtatcgggc gaaggggatc aggccggaag gccggagatc
     7201 gccttcattt ccatgaagtc cttcaggccg gccagaccgc cttcgcggcc gttgccggac
     7261 tgcttgtacc cgccgaacgg cgcgcccggg gtggggcccc agttgttgat cgcaaccatg
     7321 ccggcgcgca gctggccggc cacttcggca gccttggccg gatcgccggt gatcgcggcg
     7381 gagaggccgt attcggtatc gttggccagc tcgatgccct gctccaggct gtcataggtc
     7441 atgatcgtgg cgaccggtcc gaagatttcc tcctgcgcga tccgcatgtc cggggtcacg
     7501 cccgagaaca cggtcggctg gatgtagtag ccgcggttga cgttcggcgg caggtccgga
     7561 ccgccggctt ccagcttggc gccttcgtcg atcgccgact ggatcaggct gcggaccttt
     7621 tcgaactggg ccttgttgac caccgggccg aggtgattgc cttcggccat cggatcaccc
     7681 accggggtgg ccgcatacat cgccttgtag aagccgacga cttcggcctc gcggtccttg
     7741 gcgaccagca ccttggtcgg gctgatgcac gactggccgg tgttgaccag cgggcccatc
     7801 gccacggccg gcaggtgggt ggcaaggtcc gcatcgggca ggaccaggtt ggggctcttg
     7861 ccgcccagtt cctggtggac gcgcttgacg gtcggcgcgg cggcgatcgc cacctggatc
     7921 ccggcgcggg tcgagccggt gaagctgacc atttcgacgt cggggtggct ggagatcgca
     7981 ttgccggtgg tcgggccatc gccctggacc aggtagaaca cgcccggcgg cacgccggcg
     8041 gcgtccagga tctcggcaaa gatcgccgcg tttgtcgggc attct
//
LOCUS       DASUDV010000492         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_966566,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000492 DASUDV010000000
VERSION     DASUDV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966566"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..372)
                     /locus_tag="VFV30_10700"
     CDS             complement(19..372)
                     /locus_tag="VFV30_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX4848602.1"
                     /translation="MTGRAEREAHGRRGEALAAWYLRFKGWRILAQRVKTPRGEIDLV
                     ARRGRTVAFVEVKWRARGADLDHAIDAYRLRRVAAAAEAVAARYAKADDVQRIDVLLL
                     APGRFPRHMANALLL"
     gene            complement(369..1196)
                     /gene="rsmI"
                     /locus_tag="VFV30_10705"
     CDS             complement(369..1196)
                     /gene="rsmI"
                     /locus_tag="VFV30_10705"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX4848603.1"
                     /translation="MDQPLAPGLYIVATPIGNLGDLSRRAATVLGAVDAVACEDTRVT
                     GKLMQHIGHKARLIRYDDHASEATRDHLLDLARSGPVALVSDAGTPLISDPGYKLVRA
                     AREGGIAVTSIPGPSAAVVALTLAGLPSDRFLFAGFLPNKAKARADTLAELAAIKATL
                     VFYETAPRLTDALAAIGEVLPGREVAVARELTKLYEECRSGSPDDLIAHYSAHPPRGE
                     IVLVIAPPGEVQTSADDADAMLREALATLKPSQAAAEVARATGLDRKALYARALELK"
     gene            1236..2387
                     /locus_tag="VFV30_10710"
     CDS             1236..2387
                     /locus_tag="VFV30_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX4848604.1"
                     /translation="MIEARMNRRGLVLLAGVALLGGCKVIPKGADTTPAPEPAPTDSL
                     PKDQARHRIALLVPLSGPNEALGNSIANATTMAILDTNAQTIRITTYDTASGPAAAAS
                     QAVKDGNKLILGPLLADDVGTVAAIARPARVPLITYSNEGSVASRDVFVMGHTVESSV
                     SRVVRFAASKGVTRFAVLAPKGDYGARATAAYTVAVRAVGGTLVASESYDRGNNSIVS
                     ATARLKAKGSAQAVLIADSPRYAALAAPRLKSAGAAGPRILGTEMWSGDPLASSAPAL
                     RGAWFAAVSDTRFRQFSTSYKARFGVAPYRLATLGYDSVLLTVRIARDWRPGTVFPIG
                     RLVDSGGFLGLDGPFRFAAGGNGDRALEVRETRAGGLTVISPAPEKFVD"
     gene            2446..4440
                     /gene="parE"
                     /locus_tag="VFV30_10715"
     CDS             2446..4440
                     /gene="parE"
                     /locus_tag="VFV30_10715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX4848605.1"
                     /translation="MSQSPDDLFDKLPAGSGESYDGSAIEVLEGLEPVRRRPGMYIGG
                     TDERAFHHLAAEVLDNAMDEAVAGHANRIEVTLEEGNRLTITDNGRGIPVDEHPKYPG
                     KSALEVILTTLHSGGKFSGKAYATSGGLHGVGVSVVNALSTLTRIEVARNKELYAQEF
                     SRGAPLGPLQHLGGTPNRRGTSVTFVPDAEIFGEDAKFKPARLFKLVRSKAYLFAGVE
                     IRWKCAASLASEDVPAEAVFQFPGGLADHLKEQIGARECVTTQFFSGSQDFPGEQQGR
                     VEWAIAWPLWSDGSYSYYCNTIPTPDGGTHEQGLRAALTKGIRAFGELVGQKKAKDIA
                     PEDIVTGSEVMLSVFIRDPQFQSQTKDRLTSPEAARMVENAVRDHFDHFLADNMERGK
                     ALLGAVMEKMDERLRRKAEREVKRKTATNARKLRLPGKLTDCTGEGDAETELFIVEGD
                     SAGGSAKQARDRKTQAILPIRGKILNVASATADKIRANSEIADLALALGCGMRKDCNT
                     DNLRYDRIIIMTDADVDGAHIATLLMTFFFQEMPDVVRRGHLYLAQPPLYRLTAGKES
                     AYARDDAHRAELEATVFKGKKVEVGRFKGLGEMNPQQLRETTMDPKSRGLLRITLPPE
                     YEGRAAVKDLVDRLMGKDPAQRFMFIQNRAAEIDPDLIDA"
     gene            4574..>5286
                     /locus_tag="VFV30_10720"
     CDS             4574..>5286
                     /locus_tag="VFV30_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4848606.1"
                     /translation="MRFEGTSNYVATDDLKVAVNAAVLLRRPLLVKGEPGTGKTVLAH
                     EIAKAVDAPLIEWNVKSTTKAQQGLYEYDAVARLRDGQLGDERVHDISNYIKKGKLWE
                     AFTSEKLPVLLIDEIDKADIEFPNDLLQELDRMSFDVYETKERIAAKERPIVVITSNN
                     EKELPDAFLRRCFFHYIKFPDRETMQAIIDVHFPGIQKMLVSRALDLFYDVRDVPGIK
                     KKPSTSELLDWLKLLLHED"
BASE COUNT          923 a         1840 c         1734 g          789 t
ORIGIN      
        1 atgcggggtc ggctccaatc acagcaacag ggcgttcgcc atgtggcgtg ggaagcgccc
       61 cggcgcaagg agcagcacgt cgatccgctg cacatcgtcc gcttttgcat agcgcgccgc
      121 caccgcttcg gccgccgcgg caacccggcg caggcggtag gcatcgatcg cgtggtccag
      181 atccgccccc cgggcccgcc atttgacctc gacgaaagcg accgtccggc cgcgccgcgc
      241 caccagatcg atttcgccgc gcggggtctt cacccgctgg gccagaatgc gccagccctt
      301 gaaccgcaga taccacgccg ccagcgcctc gccgcggcgc ccatgggcct cgcgctcggc
      361 ccggccggtc acttcagttc cagcgcccgg gcatagagcg ccttgcggtc cagcccggtg
      421 gcccgcgcca cctcggccgc ggcctggctg ggtttgagcg tggccagcgc ctcgcgcagc
      481 atggcgtctg cgtcgtccgc gctggtttgc acttcgcccg gcggggcgat caccagcacg
      541 atttcgcccc tggggggatg ggcgctgtaa tgcgcgatca ggtcgtccgg cgatccgctg
      601 cggcattcct cgtacagctt ggtcagctca cgcgcgacgg ccacttcccg cccgggcagg
      661 acctcgccga ttgccgccag ggcatcggtc agccgcggag cggtttcata gaacaccagg
      721 gtcgccttga tcgcggccag ttcggccagg gtatcggccc gggccttggc cttgttgggc
      781 agaaagccgg cgaagaggaa cctgtcgctc ggcaggccgg ccagggtcag cgcaaccacc
      841 gccgcgctgg gtccggggat cgaggtgacg gcaatgcccc cctcccgcgc ggcgcggacc
      901 agcttgtagc ccgggtcgga gatcagcggc gtgccggcat cgctgaccag cgccaccggc
      961 ccggaccggg ccagatcgag caggtggtcg cgggtcgcct cgctggcgtg gtcgtcataa
     1021 cggatcaggc gcgccttatg gccgatgtgc tgcatcagct tgccggtcac gcgggtatct
     1081 tcacaggcca ccgcatccac ggcaccgagt accgttgcgg cgcggcgcga caggtcgccc
     1141 agattgccaa tcggggtggc gacaatgtag aggccggggg caagcggttg atccatcgga
     1201 actaccatgg actgccggca cgggagcgac aaggtatgat cgaagcgcga atgaaccggc
     1261 gggggctggt cttgctggcg ggcgtggccc tgctgggcgg gtgcaaggtc atacccaagg
     1321 gagccgacac cacccctgcc cccgaaccgg caccgaccga cagtctgccc aaggaccagg
     1381 cccgccaccg tatcgcgctc ctggtgccgc tgagcggccc gaacgaggcc cttggcaatt
     1441 cgatcgccaa cgccaccacc atggcgatcc tcgataccaa cgcgcagacg atccggatca
     1501 cgacctatga tacggccagt ggccccgctg ccgccgccag ccaggccgtc aaggacggca
     1561 acaagctgat cctcggcccg ctgctggccg acgatgtcgg tacggtcgcg gccatcgcgc
     1621 gcccggcccg ggtgcccctg atcacttatt ccaacgaagg ctcggtcgcg tcgcgcgatg
     1681 tcttcgtgat gggccacacg gtggaaagct cggtcagccg ggtcgtccgc ttcgccgcgt
     1741 caaagggcgt gacccgcttt gccgtactgg cgccgaaggg cgattacggc gcccgtgcca
     1801 cggccgccta taccgtcgcc gtgcgcgcgg ttggcggaac gctggtcgcc agcgaaagct
     1861 atgaccgcgg caacaattcg atcgtcagcg ccaccgcccg cctcaaggcc aagggcagcg
     1921 cccaggcggt gctgatcgcc gattccccgc gctatgccgc gctggccgcg ccgcgcctga
     1981 aaagcgccgg ggccgccggt ccgcggatcc tggggaccga aatgtggagc ggcgatccgc
     2041 tggccagcag cgcgccggcg ctgcgcgggg cctggttcgc ggcggtgtcc gacacccggt
     2101 tccgccagtt cagcaccagc tacaaggcgc gcttcggcgt ggcgccctac cgcctggcga
     2161 cgctgggcta tgacagcgtt ctgctgaccg tgcggatcgc gcgggactgg cggcctggca
     2221 ccgtcttccc catcggccgc ctggtcgaca gcggcggctt cctggggctc gacgggccgt
     2281 tccggttcgc cgcgggcggc aacggcgacc gcgcgctgga agtgcgcgag acccgcgccg
     2341 ggggcctgac cgtgatcagc cccgcgcccg agaagttcgt ggactgacga taacggggga
     2401 ctggcgcttg cacgccggat tcccggcgtt ctataggcgt tccccatgtc ccaatctccc
     2461 gacgacctgt ttgacaagct gcccgccggt tcgggcgaat cctatgacgg cagcgcgatc
     2521 gaagtgctgg aagggctgga gccggtccgg cgccgccccg gcatgtacat cggcggcacg
     2581 gatgagcgcg cctttcacca cctcgccgcc gaagtgctcg acaacgcgat ggacgaggcc
     2641 gtggccggcc acgccaaccg gatcgaagtg acgctggagg aaggcaaccg cctgaccatc
     2701 acagacaacg gccgcggcat cccggtggac gagcatccca agtacccggg caagtcggcg
     2761 ctggaagtga ttctgaccac gctccactcc ggcggcaagt tctccgggaa agcctacgcc
     2821 acctcgggcg gtctccacgg cgtcggcgtc tcggtggtca acgcgctctc caccctcacc
     2881 cggatcgaag tggcccggaa caaagagctc tacgcccagg aattctcgcg gggggcaccg
     2941 ctcgggccgc tccagcacct gggcggcacg cccaaccggc gcggcacctc ggtgacgttc
     3001 gtccccgatg cggaaatctt tggcgaggat gcgaagttca agccggcccg gctgttcaag
     3061 ctggtccggt ccaaggccta cctgttcgct ggggtggaaa tccgctggaa atgcgccgcc
     3121 tcgctcgcca gcgaggacgt gccggccgag gccgtgttcc agttcccggg cggtctggcc
     3181 gatcacctca aggaacagat cggcgcgcgc gaatgcgtga ccacgcagtt cttttccggc
     3241 agccaggact ttcccggcga gcagcagggc cgggtcgaat gggcgatcgc ctggccgctg
     3301 tggtcggacg ggtcctactc ctactactgc aacaccatcc ccactcccga tggcggcacc
     3361 cacgaacagg gtctgcgcgc cgcgctgacc aagggcatcc gcgcctttgg cgagcttgtc
     3421 gggcagaaga aggccaagga catcgcgccg gaggacatcg tcaccggcag tgaagtgatg
     3481 ctttcggtct tcatccgcga tccccagttc caaagccaga ccaaggaccg gctgaccagc
     3541 ccggaagccg cccgcatggt cgagaacgcg gtgcgcgatc acttcgacca tttcctcgcc
     3601 gacaacatgg agcgcggcaa ggcgctgctg ggcgcggtga tggagaagat ggacgagcgg
     3661 cttcggcgga aagcggaacg cgaggtcaag cgcaagaccg ccaccaacgc ccgcaagctc
     3721 cgcctgccgg gcaagctgac cgattgcacc ggcgaaggcg atgccgagac cgagctgttc
     3781 atcgtcgaag gggattcggc cggcggctcg gccaagcagg cccgtgaccg caagacccag
     3841 gcgattctgc cgatccgcgg caagatcctc aacgtcgcca gcgccacggc tgacaagatc
     3901 cgcgccaaca gcgaaatcgc cgatctggcg ctcgcgctcg gctgcggcat gcgcaaggac
     3961 tgcaacaccg acaacctgcg ctatgaccgg atcatcatca tgaccgacgc ggacgtcgac
     4021 ggcgcccaca tcgccacctt gctgatgacc ttcttcttcc aggagatgcc ggacgtggtc
     4081 cggcgcgggc acctctacct tgcccagccg ccgctttacc gcctgaccgc cggcaaggag
     4141 agcgcctatg cccgcgacga cgcgcaccgc gcggagctgg aagccaccgt gttcaagggc
     4201 aagaaggtgg aagtcggccg gttcaagggc ctgggcgaaa tgaaccccca gcagctgcgc
     4261 gaaaccacca tggaccccaa gtcgcgcggg ctgctgcgga tcaccctgcc ccccgaatac
     4321 gaaggccgcg ccgcggtgaa ggacctggtc gaccggctga tgggcaagga ccccgcccaa
     4381 cgcttcatgt tcatccagaa ccgcgcggcg gaaatcgacc cggacctgat cgacgcctga
     4441 tcgggctagg tccgacaacg gtgtttgtcg gaccgctgac gcaggtcgaa cagaatccgc
     4501 tgacgggtct tgcacgccgc cgcgcgctcc cctatcccgc ttaaccgcac gattaaacgg
     4561 acagggagtt cccatgcgct tcgaaggcac cagcaactat gtcgcgaccg atgacctgaa
     4621 ggttgccgtc aacgccgcag tcctgctgcg ccggccgctg ctggtgaagg gcgagccggg
     4681 caccggcaag acggtgctgg cccacgagat cgccaaggct gtcgatgcgc cgctgatcga
     4741 atggaacgtc aagtcgacca ccaaggcgca gcagggcctc tatgagtacg acgccgtggc
     4801 ccgtctgcgt gacggccagc tgggcgacga gcgggtccac gacatttcga actacatcaa
     4861 gaagggcaaa ctgtgggagg ccttcacctc cgagaagctg cccgtcctgc tgatcgacga
     4921 gatcgacaag gccgacatcg agttcccgaa cgacctgctg caggagctcg accggatgag
     4981 cttcgacgtc tacgagacca aggagcggat cgcggccaag gagcgcccga tcgtcgtcat
     5041 cacctcgaac aacgagaagg aactgcccga cgccttcctg cgccggtgct tcttccacta
     5101 catcaagttc cccgaccgcg agacgatgca ggcgatcatc gacgtccact tccccgggat
     5161 ccagaagatg ctggtcagcc gggctctcga tctcttctac gacgtgcgcg acgttcctgg
     5221 aatcaagaag aagccttcga ctagcgagct gctggattgg ctcaagcttc tcctgcacga
     5281 ggacat
//
LOCUS       DASUDV010000493         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1386103,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000493 DASUDV010000000
VERSION     DASUDV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386103"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1179)
                     /locus_tag="VFV30_10725"
     CDS             complement(1..1179)
                     /locus_tag="VFV30_10725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4848607.1"
                     /translation="MKPLRVLSLSTLYPNDQTPNFGVFVERQMQAVARRGDVDLVMVN
                     PIGVPPFPLSLLPRYRQLTRLPDSEERGGVGVLRPRFRLGPGPLGRFNAAAEAGAVLS
                     LARRLHASAPFDLVDAQFFWPDGPAALKVARALGLPVSIKARGADVHYWGEARGIAAQ
                     VRGAAREADGLLAVSAGLADDIAAWDVPRDRITIHRTGLDRAVFKPLDKAQCRAALGL
                     PQDRPVLATVGALIARKGQRHAIEALAHLPEAVLVLAGSGPDLATLRAVAEAQGVEAR
                     VRFLGAVPHGDLPQVLSAADVFVLPSASEGLANAWVEALACGTPVVTTPIPGALELLT
                     DPAWGRTAPREAAAIAEAVRAVLSQATPRDVVQQGAAEFSWEANAAALVAHWRRLAGR
                     "
     gene            complement(1176..1748)
                     /locus_tag="VFV30_10730"
     CDS             complement(1176..1748)
                     /locus_tag="VFV30_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4848608.1"
                     /translation="MINRIRDIRRSKNLTLAEVAARCKPATTAQTIGRLETGTRQLSL
                     GWMNRIAAALGVEPELLVRGDEPGQPKYVARLGEDGAEALAAPRDAILPTALSGDGVL
                     VAMMIEASAGQYRSGDQLWLRQHGPEDYARLLNRDVLVPRAGGRFAFGRMIDRDEYRV
                     AVLPPDPGQRQIVIDNPAWIAAAEMLVRAL"
     gene            1847..2317
                     /locus_tag="VFV30_10735"
     CDS             1847..2317
                     /locus_tag="VFV30_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX4848609.1"
                     /translation="MRQQLVERELTIEGPAARAPRRGRRSVTVNLAESPLAWLHARGH
                     LSDVQLAAGEALRRDWEAAQLSPGVTMRWDPVRVQGGGERGLNASERQLAARARFDGA
                     VAAAGPGLSDVLWRVVCAGETLPDAEKALAWPVRSGKLVLKLALDRVAGFYRLG"
     gene            complement(2322..>2856)
                     /locus_tag="VFV30_10740"
     CDS             complement(2322..>2856)
                     /locus_tag="VFV30_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4848610.1"
                     /translation="ARKSNLLVLDEPTNDLDLETLDLLQEVIADYDGTVLIVSHDRDF
                     LDRTVTITLGLDGSGKVDVVAGGYADWEKQRATRRIAPVKPAKPAVEVAAPPPPAPKK
                     AKLSYKDQRDYDLLPGRIEELLAQIARDEAALADPALYTSNPARFAALTKAIETARAE
                     KDAAEERWLELAELVEG"
BASE COUNT          441 a          984 c          982 g          449 t
ORIGIN      
        1 ctagcgcccg gccagtcgcc gccagtgcgc gacgagggct gcggcgttgg cttcccagct
       61 gaattcggcg gcgccctgtt gcaccacgtc gcgcggggtt gcttgtgaca gtaccgcgcg
      121 cacggcctcg gcaatcgccg ctgcttcgcg cggggcggtg cggccccagg ccggatcggt
      181 cagcagttcc agtgcgccag ggatcggggt ggtgaccacc ggagtgccgc aggccagcgc
      241 ttcgacccag gcattagcca ggccctcgct ggcgctgggc agcacgaaga catcggcggc
      301 tgaaagcacc tgcggcagat cgccgtgcgg caccgcgccc agaaagcgca cccttgcttc
      361 cacgccctgg gcttcggcca cggcgcgcaa ggtcgccaga tcggggccgc ttccggccag
      421 caccagcacg gcttcgggta ggtgcgccag cgcctcgatc gcatggcgct ggcccttgcg
      481 ggcgatcaga gcgccaaccg tggccagcac cggccggtcc tgcggcaggc caagcgctgc
      541 gcggcactgc gccttgtcga gcggtttgaa caccgcacgg tcaagcccgg tgcggtggat
      601 tgtaattctg tcgcgcggca cgtcccaggc ggcgatatcg tcggccagac cggccgatac
      661 cgccagcagg ccatcggcct cacgcgccgc accgcgaacc tgcgcggcga tcccgcgcgc
      721 ctcgccccag tagtggacat cggcgccgcg cgccttgatc gaaaccggga ggcccagtgc
      781 ccgcgccact ttgagcgcgg ccgggccatc gggccagaag aactgtgcat cgaccagatc
      841 gaacggcgca gaggcatgaa gccgccttgc cagcgacagg actgcgccag cctctgccgc
      901 tgcattgaac cgccccagag gcccgggacc aagccggaaa cgggggcgca gtacccccac
      961 gccgccgcgc tcctcgctat cgggaagcct tgtcagctgg cgatagcgcg gcagcagcga
     1021 aagcgggaag ggtggcacgc cgatcgggtt gaccatcacc agatcgacat cgccgcgcct
     1081 ggccacggcc tgcatctgcc gctccacgaa gaccccgaaa ttcggggtct gatcgttggg
     1141 atagagcgtc gatagggaaa gtacccgcaa gggcttcaca gcgcgcgcac cagcatttcc
     1201 gccgcggcga tccaggccgg gttgtcgatc acgatctggc gctggccggg atcggggggc
     1261 agcaccgcca cccggtactc gtcgcggtcg atcatccgtc cgaaggcaaa ccgtccaccc
     1321 gcgcgcggca ccagcacatc gcggttgagc agccgggcat agtcctccgg gccatgctgg
     1381 cgcagccaca gctgatcgcc gctgcgatac tggcctgcgc ttgcctcgat catcatggcc
     1441 accagcacgc catcgccgct cagcgccgtg ggcaggatcg catcgcgcgg ggcggccagt
     1501 gcctcggccc cgtcctcgcc cagccgggcg acatatttgg gctgaccagg ctcgtcccca
     1561 cgcaccagca gttcgggttc gacccccagc gcggcggcga tccggttcat ccagccaagc
     1621 gaaagctgcc gggtgccggt ttcgagccga ccgatcgtct gcgcggtggt ggccggcttg
     1681 cagcgcgctg ccacctcggc aagcgtcagg ttcttcgaac ggcggatatc gcggatgcgg
     1741 ttgatcatgc tgggtctcga ttaaccagat tggtgttttc gctttcctac aaagccgccg
     1801 ctttggcaag ccttgccacg actcaactgc gcgaaaggga acagcgatgc ggcagcaact
     1861 ggtggaacgc gaactgacga tcgaaggccc ggcggcccgt gctccgcggc gtggcaggcg
     1921 ctcggtcacg gtcaacctcg ccgaaagccc gctggcctgg ctgcatgcgc gcggccacct
     1981 gtccgacgtg caactggccg ccggcgaagc gctgcgtcgc gattgggagg cggcgcagct
     2041 ttcccccggg gttaccatgc gctgggatcc ggtgcgggtg cagggcggcg gcgagcgcgg
     2101 cctgaacgcc agcgaacgcc agctggccgc gcgggcccgc ttcgatggag cggtggctgc
     2161 cgccggaccg ggcctcagcg atgtgttgtg gcgcgtggtc tgcgccggcg aaacgctgcc
     2221 cgatgctgaa aaggccctgg cctggccggt gcgcagcggc aagctggtgc tgaaactcgc
     2281 gctcgatcgg gtggcggggt tttatcggct ggggtgaggg cctacccctc aaccagctcc
     2341 gccagttcca gccagcgttc ttcggccgcg tccttttcgg cgcgggcggt ttcgatcgcc
     2401 ttggtcagcg cggcgaacct tgccggattg ctggtgtaga gcgcgggatc ggccaatgcg
     2461 gcctcgtcgc gggcgatctg cgccagaagt tcctcgatcc ggcccggcag cagatcgtaa
     2521 tcgcgctggt ccttgtagga tagcttggcc ttcttcggcg cgggcggcgg gggcgcggcc
     2581 acttcgaccg ctggcttggc cggcttgacc ggggcgatcc gccgcgtggc ccgctgcttt
     2641 tcccaatcgg catagccgcc cgcgaccaca tcgaccttgc ccgaaccatc aaggccaagc
     2701 gtgatcgtga ccgtgcgatc gagaaagtcg cggtcgtggc tgacgatcag caccgtgcca
     2761 tcgtaatcgg cgatcacttc ctggagcagg tcgagcgttt caagatcaag gtcgttggtc
     2821 ggctcatcga gcaccagcag gttcgacttg cgggca
//
LOCUS       DASUDV010000494         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1828384,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000494 DASUDV010000000
VERSION     DASUDV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828384"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..999
                     /locus_tag="VFV30_10745"
     CDS             40..999
                     /locus_tag="VFV30_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4848611.1"
                     /translation="MSSHLSILPAILVASLPLPAAARADAKHAVGFETIAMEDGGELA
                     IWYPAKGEAKSQRLGLYLQRVVTGADPVGRKLPLIVISHGSGGTYAAHHDTAVALAES
                     GFIVAAPLHPGDNWRDRSRATDLAARTARISAAIDTVFSRWSHGALVNPRKIGAMGYS
                     AGGFAVLAAAGARPDLARIDAHCQAVPAAFECGLAGARSVPQSPWLAIPDRRIRALVL
                     AAPALGYTFAGGLEEVRIPVQLWRAGKDALLPAPHYADAIRAALPRRPEYHDVPGAGH
                     FDFMAPCTAPGARAPICETQEFDRAEFHRSYNASITRFFQKHL"
     gene            1255..>2185
                     /locus_tag="VFV30_10750"
     CDS             1255..>2185
                     /locus_tag="VFV30_10750"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX4848612.1"
                     /translation="MVTRFAKGQKLNVGIVGATGVVGSTMLAILKERNFPIGQLRLFA
                     SARSAGKVIDGVTIEDAASADYAGLDVVLFSAGGSTSKALAPRVAAAGAVVIDNSSAW
                     RSDPEVPLVVAEVNPHAMANLPKGIIANPNCTTMAAMPAIKPLHDEAGLKRIVASTYQ
                     AVSGAGLEGIDVLARQLEHVGDKARLLAEGGHDDLLPPAEKWMVPMAHNVVPLNYVLG
                     EDDYTEEELKLRDESRKILEIPNLPVSGTCVRVPVFTGHSIALNLEFERPISAARALE
                     LLGAAPGVVLSQCPNPLEATGKDPVYVGRVRVDR"
BASE COUNT          364 a          718 c          716 g          387 t
ORIGIN      
        1 cgaacaagat gggggcatcc ttttgcgtgg agaccccact tgtccagcca tctttcgatc
       61 cttccggcga tccttgtcgc cagtctgccg cttcccgccg ctgcccgtgc cgacgccaag
      121 cacgcggtcg ggttcgaaac catcgccatg gaagatggcg gcgaactggc gatctggtat
      181 ccggccaagg gcgaggcaaa atcgcagcgt ttgggcctct atctgcagcg ggtggttacc
      241 ggcgccgatc cagtcggccg gaagctgccg ctgatcgtga tctcgcatgg cagcggtgga
      301 acctatgccg cgcatcacga taccgctgtc gccctggccg agtccgggtt catcgttgca
      361 gccccgcttc atcccggcga caactggcgc gaccgcagcc gcgcaaccga tcttgccgcc
      421 cggaccgcgc ggatttccgc cgcgatcgac acggtgtttt cgcgctggtc gcacggcgct
      481 ttggtcaatc cgcgcaagat cggggcgatg ggctattcgg caggaggatt tgcagtgctg
      541 gcggcagctg gcgcgcggcc agatctggcg cggatcgatg cccattgcca ggccgtgccc
      601 gcggccttcg aatgcggcct tgccggcgcg cgttcggtgc cgcaaagtcc ctggctggca
      661 atccccgatc gccggatcag ggcgctagtg ctggcagctc cggcgctggg ctataccttt
      721 gccggcggcc ttgaagaggt gaggatcccc gtgcagctgt ggcgcgccgg caaagacgca
      781 ctgctgcccg cgccgcatta tgccgatgcg atccgcgccg ccctgccgcg ccggcccgaa
      841 taccatgacg tgcccggcgc aggccatttc gatttcatgg caccctgcac cgcgcccggg
      901 gccagggcgc cgatctgcga gacccaggag ttcgatcgtg cggagttcca ccgcagctat
      961 aacgcgagca tcacccggtt cttccaaaag cacctatagc ccgcgcgcca caaagtttcg
     1021 cgggctgatc ggcggctgac tttacattgc gcgaaaccgc cgctagaggc gcctcccatg
     1081 atcacgccga tgccgattac cactattacc aaaccggccg ctttcgcggg cccggttgtg
     1141 gtggtgtttg gctaaggcac ccgatcaacc cgccccgcac gagcaggcgg ggcccatttt
     1201 ccagaccgga tcaaaccctc gcgctgctct cctgaataag aaggagttgc tttgatggtt
     1261 acgcgtttcg ccaagggaca aaagctcaac gtcggtattg tcggcgccac cggggtggtc
     1321 ggatcgacca tgctggcgat cctgaaggaa cggaacttcc cgatcgggca gctgcgcctg
     1381 ttcgcctcgg cgcgttcggc gggcaaggtg atcgacgggg tgacgatcga ggatgcggca
     1441 agcgccgact atgccgggct cgacgtggtg ctgttcagcg ccggcggttc gacctcgaag
     1501 gcgctggccc ccagggtggc cgctgccggc gcggtggtga tcgacaattc ttcggcctgg
     1561 cgttccgacc cggaagtgcc gctggtggtg gccgaagtga acccgcatgc gatggccaac
     1621 ctgcccaagg ggatcattgc caatcccaac tgcaccacca tggccgcgat gcccgcgatc
     1681 aagccgctgc atgacgaggc gggcctgaag cggatcgttg ccagcaccta tcaggcggtg
     1741 tcgggcgcgg ggttggaagg gatcgacgtg ctggcccgcc agctcgagca tgtgggcgac
     1801 aaggcccgcc tgctggccga aggcgggcac gacgatctgc ttcccccggc ggaaaagtgg
     1861 atggtgccga tggcccacaa tgtggtgccg ctcaactacg tgctgggcga ggacgactat
     1921 accgaggaag agctcaagct gcgcgatgaa agccgcaaga tccttgaaat tccgaacctg
     1981 ccggtttcgg gcacctgcgt gcgcgttccg gtgttcaccg ggcattcgat tgcgctcaac
     2041 ctcgaattcg agcgcccgat cagcgcggct cgtgcgctcg aactgctagg agctgcaccc
     2101 ggcgtggtgc tgagccagtg ccccaacccg cttgaggcga ccggcaagga tccggtctac
     2161 gtcggccgcg tccgcgtcga ccgca
//
LOCUS       DASUDV010000495         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2093327,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000495 DASUDV010000000
VERSION     DASUDV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093327"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFV30_10755"
     CDS             <1..528
                     /locus_tag="VFV30_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848613.1"
                     /translation="VEAFRAAQIARNRRITARAQAMLEEIRASSLPDGERCFTVQGTM
                     SDLRWLDPTQDPSDREPYTCYLGDPRLVNDGPVGLGRFTTARSWLSQWSYDLSNANGL
                     KNGAGVSVPALVLNNTADLACTPSHAARLFDCLASKDKTYRDVQGADHYYIARPDLLP
                     EAVGIVQGWMGERGF"
     gene            complement(525..1226)
                     /locus_tag="VFV30_10760"
     CDS             complement(525..1226)
                     /locus_tag="VFV30_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX4848614.1"
                     /translation="MCVAAIAWAAHPRWQLVVAGNRDEFHARPTAALARWDEGLLAGR
                     DLEAGGTWMGIAPGRFALVTNRRAEGYPRLGMASRGELVSGWLLGAEPAQTARMNPFN
                     LFLADQGQARMLSNFPEQSHDVPHGSLLSLSNSDLADRWFKAGRIESGLLTWLGGEED
                     PEALLAALADEVRDPADPDAIHSSVFSRNPTYGTRASSVIAIDQAGRGVFIERSFNNT
                     GAETGTVRLKFDWNV"
     gene            complement(1226..1918)
                     /gene="radC"
                     /locus_tag="VFV30_10765"
     CDS             complement(1226..1918)
                     /gene="radC"
                     /locus_tag="VFV30_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX4848615.1"
                     /translation="MADADDNSHHGTGHRARLRSRLLAGGEAALADHEVIEYLLMTAI
                     PRRDVKPLAKSLLARFGGLAGVFNADPAALAKHPGMGETSAAALKIVALATRRLARTG
                     VQESPVLSSWQALIEYLTIDMAHLKHERVRVLYLDTRNRLVLDDLLTDGSLDEAAIHP
                     REVVKKALDVGAAALILVHNHPSGSPEPSRADVQITNRIAEAGRLLGITVHDHVIIGK
                     EGHVSLKAKGLV"
     gene            1992..>2436
                     /locus_tag="VFV30_10770"
     CDS             1992..>2436
                     /locus_tag="VFV30_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX4848616.1"
                     /translation="MSEVADEIPDLAEETASPRATSRRRRWLAYVGAALVLGLGGVWL
                     VRERLANRIIADQLESYGLPGKYEIEKIGVGRQVLRKVVIGDPARPDFTADEVTIETD
                     LALGLPTFGRITLVRPRLYGSYTKGKLSFGALDKVFFAKSERPPGL"
BASE COUNT          396 a          808 c          806 g          426 t
ORIGIN      
        1 gtcgaagcat tccgcgccgc gcagattgcc cgcaaccggc ggatcaccgc gcgggctcag
       61 gcgatgctgg aggaaatccg tgcctcaagc ctgcccgacg gtgagcgctg ctttaccgtc
      121 cagggcacga tgagcgacct gcgctggctc gatccgaccc aggacccctc ggaccgcgaa
      181 ccctatacct gttacctggg cgatccccgg ctggtgaacg atggcccggt tggcctgggc
      241 cgcttcacca ccgcgcggtc atggctgtcg caatggtcct atgacctctc gaacgccaat
      301 ggcctcaaga acggtgctgg cgtcagcgtt ccggcgctgg tgctcaacaa caccgccgat
      361 ctcgcctgca cccccagcca cgcggcgcgg ctgttcgatt gcctggcgag caaggacaag
      421 acctaccgcg atgttcaggg cgccgaccac tattacatcg cccggcccga cctgctgccc
      481 gaggcggtgg ggatcgtcca gggatggatg ggcgaacgcg ggttttagac gttccagtcg
      541 aatttaagcc gcaccgtgcc cgtttccgcg ccggtattgt tgaagctgcg ctcgatgaac
      601 accccgcggc ccgcctgatc gatcgcgatc acgcttgagg cgcgggtgcc gtaggtgggg
      661 ttgcggctaa agaccgagga atggatcgca tcgggatcgg cgggatcgcg cacctcgtcg
      721 gccagcgcgg cgagcagggc ttcgggatcc tcctcgccgc ccagccaggt cagcagcccg
      781 ctttcgatcc ggccagcctt gaaccacctg tcggcaaggt cgctgtttga caagctgagc
      841 aaggaaccgt gcggaacatc atgcgattgt tcgggaaaat tgctgagcat ccgtgcctgc
      901 ccctggtctg ccaggaacag gttgaacggg ttcatccttg cggtttgcgc cggttccgca
      961 cccagcaacc agccgctgac cagttccccg cgcgaggcca ttccaaggcg cggatagcct
     1021 tcggcgcggc gattggtgac cagtgcgaag cggcccggcg cgatccccat ccaggtgccg
     1081 cctgcctcaa gatcacggcc tgccagcaga ccctcgtccc agcgtgccag cgcggcggtg
     1141 ggacgggcat ggaattcatc gcggttgccg gcaacaacca gttgccagcg cggatgcgcg
     1201 gcccacgcga ttgctgcgac gcacattaca cgagcccctt cgccttcagg ctgacatggc
     1261 cttccttgcc gatgatcacg tgatcgtgaa ccgtgatccc cagcaatcgc cccgcttcgg
     1321 cgatgcggtt cgtgatctgc acgtcggcgc ggctcggttc gggcgagccc gagggatggt
     1381 tgtggaccag gatcagcgcc gccgccccca catcaagcgc cttcttcacc acttcgcgcg
     1441 ggtggatcgc ggcttcatcg aggctgccgt cggtaagcag atcgtcgagc accaagcggt
     1501 tgcgggtatc gagatagagc acccgcaccc gttcgtgctt gaggtgcgcc atgtcgatgg
     1561 tgaggtattc gatcagcgcc tgccagctcg acagcaccgg gctttcctgc actccggtgc
     1621 gagccagtcg gcgggttgcc agggccacga tcttgagcgc ggcggcgctg gtttcgccca
     1681 tgccgggatg cttggccagc gcggccggat cggcgttgaa caccccggcc agcccgccaa
     1741 agcgcgccag caggcttttc gccagcggct tcacgtcgcg ccggggaatt gcggtcatca
     1801 gcaggtattc gatcacctcg tgatcggcga gcgcggcttc gccgccagcc agcagccgcg
     1861 agcgcagccg agcgcggtgc ccggtcccat ggtgcgagtt gtcgtctgcg tcggccaagc
     1921 ggtgatcctg cggcgtgaac ggtgcggcgc tttcttgcct ttcacggccc gaacgcgcaa
     1981 gggtaggcac gatgtccgag gttgccgacg aaattcctga cctggctgaa gaaacggcat
     2041 cgccccgcgc cacctcccgg cggcggcgct ggctggccta tgtcggggcg gcgttggtgc
     2101 tgggccttgg cggcgtctgg ctggtgcgcg aacggctggc caaccggata atcgccgatc
     2161 agcttgaaag ctatgggctg cccggcaagt acgagatcga aaagatcggc gtgggccggc
     2221 aggtgctgcg caaggtagtg atcggcgatc ccgcccggcc cgatttcacc gccgatgaag
     2281 tgacgatcga aaccgatctg gcgctggggc tgcccacctt cgggcggatc acgctggtcc
     2341 ggccgcggct ctatggcagt tacaccaagg gcaagctcag cttcggtgcg ctcgacaagg
     2401 tgttcttcgc caagagcgag cggcccccgg ggctgc
//
LOCUS       DASUDV010000496         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2093334,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000496 DASUDV010000000
VERSION     DASUDV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093334"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..946)
                     /locus_tag="VFV30_10775"
     CDS             complement(146..946)
                     /locus_tag="VFV30_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX4848617.1"
                     /translation="MSLDLEYDESGEDPPATPAATLVIFRRDAAGGAPQILMVERSAT
                     MKFAGGAAVFPGGRVDDADRELAQALGAADEEAIHELAARIAAVRETLEETGLVVGIR
                     QRPSLDEARRAREILLAEGTLAPTLAAMGWELDLDALVPFARWRPKHRHLRVYDTRFY
                     LADLGTGAVDLLVDETENTRLFWTGAAEALEHADAGRISVIFPTRRNLERLAQFGSFD
                     EAVAHVEVYPSRMITPFLTEEDGQKWLRIPHDAGYPVTGEVLESAARG"
     gene            1339..>2250
                     /locus_tag="VFV30_10780"
     CDS             1339..>2250
                     /locus_tag="VFV30_10780"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX4848618.1"
                     /translation="MSQANADYESKPRSWLFGPMAGLAAGALGLLVLLSVFLIQRFDR
                     AATEREEHMVQNGMAVVAAELNRVVATQVEWDGAVAHLDNRFDANWADFNIGNYLYVF
                     HGFSHAYVLGSDDRVVYASTNGERAPLTDYERFQTAAESLLPEVRAQERARPKPGKRP
                     GKNNIVIPSIQANGITLIDGLPFIISATLVQPDFGESLPKGPRAPIAIAVKPIDQAML
                     KSFADRYLLDDLAVRSPQAVAASDGHLLLANRAGEPIAALTWTPQRPGTMLLVQLAAP
                     LVLLLALLAALAWRVIQRGSAIATDLIA"
BASE COUNT          354 a          751 c          718 g          427 t
ORIGIN      
        1 gcgtgatctg catcacgcct gtgttccggc gaatcccata gaaacggcgt gcgacccacc
       61 ggcgcgagcc tttccgccgg caactttgcc cccgtgccac cctcggcgcg ggggcttttt
      121 attgcggatc aggggtgcgg cgcgcttagc cgcgggcggc gctttccagc acttcgccgg
      181 tcaccggata gcccgcatcg tgcgggatcc gcagccactt ctgcccgtct tcctcggtca
      241 ggaagggggt gatcatccgg ctggggtaga cttcgacatg ggccacggcc tcatcgaagc
      301 tgccgaactg ggccagccgc tcaaggttgc ggcgggtcgg gaagatcacg ctgatccggc
      361 cggcgtcggc gtgctccagc gcctcggccg cgccggtcca gaacaggcgg gtgttctcgg
      421 tctcatcgac cagcagatcg accgcgccgg tgcccaggtc cgcgaggtag aagcgggtgt
      481 catagacccg caggtggcgg tgcttgggcc gccagcgggc gaagggcacc agggcatcga
      541 gatcgagctc ccagcccatc gcggcgaggg tgggggccag ggtcccttca gccagcagga
      601 tttcccgcgc gcgccgcgcc tcgtcaagcg agggacgctg gcggatgccg acgaccaggc
      661 cggtttcctc cagcgtttcg cgcacggcgg cgatccgggc ggcgagttcg tggatcgctt
      721 cctcgtccgc cgcgcccagc gcctgggcca gttcgcggtc ggcatcgtca acccggccgc
      781 cggggaagac cgcggcaccg ccggcgaact tcatcgttgc cgagcgttcg accatcagga
      841 tctgcggggc cccgccggcg gcatcgcggc ggaagattac cagagtggca gcgggggtgg
      901 cgggcgggtc ttccccgctc tcgtcatatt caaggtccaa gctcatcgtt gccaaaggtg
      961 cctgcgccgc accgctttgc caagccgctt ttgtatcagg cgccggttgt cggcaaactt
     1021 tacagggcgg gatgccgtta acttttcgct gaccatcgtc ttgcggggaa ttctgccctt
     1081 gcggcaaact ggcacgccgg ctgcatagta tctggtaccc ccaaagcagt ctcgaatgag
     1141 gcggggccgc actggtctcc gcggtcccgc ctccccgaga aaaccccaga catcgctgac
     1201 ttccgccgca aggtaccgcg cctctgcttg gtgaacaggt ccctaacctt gtcgtaaaac
     1261 ttgtttgacc ttttgcgggt accccttgcc gctggcgggc aatccgatct cgggttggtg
     1321 cgcttctgga gccggtactt gtcccaagcg aatgccgatt acgaatcgaa gccaagatcc
     1381 tggctgttcg gtccgatggc cgggctggca gctggcgcgc taggcttgct cgtgctcctc
     1441 tcggtattcc tgatccagcg tttcgatcgc gccgcgaccg agcgcgagga gcacatggtc
     1501 cagaacggca tggccgttgt cgcggccgag ctaaatcggg tggtggccac ccaggtcgaa
     1561 tgggacgggg cggtggccca tctcgacaat cgcttcgatg ccaactgggc cgacttcaac
     1621 atcggcaatt acctctatgt ctttcacggc ttcagccacg cctatgtgct cggatcagac
     1681 gatcgcgtag tctatgcctc gaccaacggg gagcgcgcgc cgttgacgga ctacgagcgg
     1741 ttccagactg cggccgaaag tctgctgccc gaggtgcggg ctcaggaacg ggcccgcccc
     1801 aagccgggca agcgcccggg caagaacaac atcgtcatcc cctcgatcca ggccaacggc
     1861 attacgctga tcgatggcct acccttcatc atttcggcaa cgctggttca gcccgacttt
     1921 ggcgagagct tgcccaaggg accgcgtgct ccgattgcga ttgccgtcaa gccgatcgac
     1981 caggcgatgc tcaaatcatt tgccgatcgc tacctgctgg atgatctggc agttcgttcg
     2041 ccccaggccg ttgccgcctc cgatggtcat ctgttgctgg caaaccgcgc cggcgagcca
     2101 atcgccgcac ttacctggac cccccaacgt cccggcacca tgctcctcgt tcagctggca
     2161 gccccgctgg ttctgttgct ggccctgttg gcggcactcg cctggcgcgt gattcagcgc
     2221 ggttcggcga ttgctacaga tctgatcgcc
//
LOCUS       DASUDV010000497         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1341968,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000497 DASUDV010000000
VERSION     DASUDV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341968"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1575)
                     /locus_tag="VFV30_10785"
     CDS             complement(1..1575)
                     /locus_tag="VFV30_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848619.1"
                     /translation="MSVELSRFLIFAGILLALVVFAATSKSFRPYIAKGPLVAFLLGI
                     SSGFPLTLLIATMTFWLAKVGIDKATIGFLVALGIPYTLKFLWAPLVDKLHIPVLSKA
                     LGQRRAWLFVIQALLLVSVWQLGASNPQPGAMANFALWAFITAFLSATQDIVIDAYRI
                     EILDDSNMAHGTASNQFGYRTGNLIAGAGTIYLASAEGLGLGWATAYGLTAFAIIPAI
                     LGALWIGPGTFVDRFANVSGMKPGQWLTEAVVNPFREFLSRDGAFLILGFVLVYKVGD
                     AMGQTMLSPMIVDLKFADLDYVSANKLWGFAALIIGSAMGAPFILWLGMGRALLISGL
                     MMMFSNLMFMVLAAVGNNYWMLVAAIATENLTSGIGLTVFATYLSGLSNIAYTATQFA
                     LLSSFAGVGRTFMAGPAGILAENLGWFGFWGVTVVAAAPGMFLLWLLWSKGYVVASVR
                     QTKEVDEAALRAPLPLMRIFGSVVAVVGLVGVLLSQSLGWAVSTAWTFLAVLIAGALI
                     AWFAPGKAAASPETPG"
     gene            1741..2901
                     /locus_tag="VFV30_10790"
     CDS             1741..2901
                     /locus_tag="VFV30_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4848620.1"
                     /translation="MSHTETLPGSAPTPGQAELARQIAAGCSRPDRGVTTVPAAVYTD
                     PQHWAREEAALFARLPQVLCPSALLPDPNMAVPHDATGRPLLITRDGDGKAHVFLNVC
                     RHRGTRLVEGQEVHCAKRMTCPYHAWTYATDGRLLALPRPDTFPGLDKNDYGLVELES
                     CEAGGLIWFAPQGGADFTDVRTLGADFDAFAMRDLHLFRRQTHTVASNWKLIMDAFLE
                     SYHVARLHAATIGPFFKDGVTSGDQIGPHQRSFVARAAELDGIDLADMAALRRIGTFA
                     YQLFPATVLVISPDYINVMALWPQGHDATLVEDFMLIPEEPQSDKASDHWERSWKLLD
                     GGVFAGEDFRAAALGQQGLATGAVPHLTLGTLEGGIRRLHEIIAEQMALAEG"
     gene            2933..>3424
                     /locus_tag="VFV30_10795"
     CDS             2933..>3424
                     /locus_tag="VFV30_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4848621.1"
                     /translation="MTSSDSRRDGAEPSPREGERIAKLLARAGISSRREVERMIADGR
                     VKLNGQVLTTPATVLKTLKGVTVDDKPVKAAEPTRIFIFHKPAGLLTAERDPAGRPTI
                     YTALRNALPEEAGRVMPVGRLDFNTEGLLILTNDGELKRAMELPATGLPRTYRARTYG
                     EITQ"
BASE COUNT          665 a         1180 c         1104 g          475 t
ORIGIN      
        1 tcaaccggga gtttccggac tagccgccgc cttccccggg gcgaaccacg cgatgagcgc
       61 gccggcaatc aacaccgcca ggaatgtcca ggccgtgctg acagcccagc caagcgactg
      121 ggacagcagc acaccaacca ggccgacaac agcgacgacc gagccgaaga tgcgcatcag
      181 cggcagcggc gcgcgcaagg cggcttcatc gacctccttg gtctgccgca ccgaagcgac
      241 gacatagccc ttgctccata gcagccagag caggaacatc cccggcgccg cggccacaac
      301 cgtcacgccc cagaagccga accagccaag attctcggcc aggatgcccg ccggccccgc
      361 catgaaggtc cgcccgaccc cggcaaagga ggacagcagc gcgaactgcg tggccgtgta
      421 ggcaatgttg gacaggcccg agagataggt ggcgaagacc gtcaggccga tgccgctggt
      481 caggttctcc gtcgcgatcg cggccaccag catccagtaa ttgttgccga ccgcggcaag
      541 gaccatgaac atcagattgg agaacatcat catcagcccg gaaatgagca gcgcccgccc
      601 catcccgagc cacaggatga aaggcgcgcc cattgccgaa ccgatgatca gcgcggcaaa
      661 gccccacagc ttgttggccg agacatagtc cagatcggcg aatttcagat cgacgatcat
      721 cgggctgagc atggtctggc ccatggcgtc gccaaccttg tagaccagca cgaagcccag
      781 gatcaggaag gccccgtcgc gcgagaggaa ttcgcggaat ggattgacta cggcctcggt
      841 cagccactgc ccgggcttca tcccgctgac attggcaaag cggtccacaa aggtgcccgg
      901 cccgatccac agggcgccca gaatggcggg gatgatggca aaggcggtca gcccataggc
      961 cgtcgcccaa cccagcccca gcccttcggc cgaagccaga tagattgtgc cggcacccgc
     1021 gatcaggttg ccggtgcgat agccaaactg gttgctggcc gtgccgtggg ccatgttgct
     1081 gtcatcaagg atctcgatcc ggtaggcgtc gatgacaatg tcctgggtgg ccgaaaggaa
     1141 cgcggtgatg aaggcccaca gcgcgaaatt ggccatcgcc cccggctgcg ggttgctggc
     1201 cccaagctgc cagaccgata ccagcagcag cgcctggatc acgaacagcc acgcccggcg
     1261 ctggcccagc gccttgctga gcaccggaat gtgcagcttg tcgaccagcg gtgcccacag
     1321 gaacttgagc gtgtagggaa tgcccagggc aaccaggaag ccgatcgtcg ccttgtcgat
     1381 cccgaccttg gcgagccaga aggtcatcgt cgcgatcagc agggtcagcg gaaagccgct
     1441 tgaaatgcca agcaggaagg ccaccagcgg ccccttggcg atgtagggcc tgaacgattt
     1501 gctggttgcg gcgaaaacca ccaaggccag aagaatcccg gcgaaaatca gaaaccggct
     1561 cagttcaaca gacattggcg ccctcttgtc ccagcaaatt ggctgtcagc ttagcagcca
     1621 gcatcgccga cggaagggcc tgcggccgcc agcgcacaaa aaagccggga cacggccgcg
     1681 caggcagggg tgtttccgat tttcgtgacg aacgcgaatt ttggcgtatc ctgcaccgcg
     1741 atgagtcaca ccgaaaccct gcccggcagt gcccccaccc ccggccaggc cgaactggcc
     1801 cggcagatcg cggccggctg ctcccgcccg gaccggggcg tcaccaccgt gcccgcggcg
     1861 gtctataccg atccgcagca ctgggcgcgc gaggaggcgg cgctgttcgc ccggttgccg
     1921 caggtgctct gcccgtccgc gctgctgccc gatcccaaca tggccgtgcc gcacgatgcc
     1981 accggacggc cgctgctgat cacccgcgac ggggatggca aggcccacgt gttcctcaac
     2041 gtctgccggc accggggcac gcggctggtc gaagggcagg aggtgcactg cgccaagcgg
     2101 atgacctgcc cctaccacgc ctggacctat gccaccgacg gacgcctcct cgccctgccc
     2161 cggccggata cgttccccgg gctggacaag aacgactacg gcctggtcga gctggagagc
     2221 tgcgaggccg gcgggctgat ctggttcgcg ccgcagggcg gtgccgactt taccgacgtg
     2281 cggaccctgg gcgccgattt cgatgccttc gcgatgcgcg acctgcacct gttccggcgc
     2341 cagacccaca ccgtggcatc gaactggaag ctgatcatgg acgcgttcct ggaaagctac
     2401 cacgtcgcgc ggctgcacgc cgccacgatc ggcccgttct tcaaggacgg ggtaaccagc
     2461 ggtgaccaga tcggcccgca ccagcgcagc tttgtcgccc gcgcggcaga gctggacggg
     2521 atcgacctgg ccgacatggc cgcgctgcgc cggatcggga cctttgccta tcagctgttc
     2581 ccggcaaccg tgctggtgat cagccccgac tatatcaacg tcatggcgct gtggccgcaa
     2641 gggcacgacg ccacgctggt cgaggatttc atgctgatcc ccgaggagcc gcaaagcgac
     2701 aaggccagcg atcactggga acgcagctgg aagctgctcg acggcggggt ctttgccggt
     2761 gaggatttcc gtgccgccgc gctcggccag cagggcctgg cgaccggggc cgttccgcac
     2821 ctgacgctcg gcacgctgga aggcgggatc cgccgcctgc acgagatcat cgcagagcag
     2881 atggcgctgg ccgaggggtg acggcgcggg cggaaccgcc tatgggcggc gcatgaccag
     2941 ttctgacagc cgccgtgatg gcgccgaacc cagcccccgc gagggcgaac gcatagccaa
     3001 attgctggcc cgggccggca tttccagccg ccgcgaggtg gagcgcatga tcgccgacgg
     3061 gcgggtcaag ctgaacggcc aggtgctgac caccccggcc accgtgctca agaccctgaa
     3121 gggcgtgacg gtggacgaca agccggtcaa ggcggccgag ccgacccgga tcttcatctt
     3181 ccacaagccc gccggcctgc tgaccgccga gcgcgatccg gccgggcgtc ccaccatcta
     3241 caccgcgctg cgcaacgccc tgcccgagga ggccgggcgg gtaatgccgg tcggccggct
     3301 ggacttcaac accgagggcc tgctgatcct gaccaacgac ggcgagctga agcgggcgat
     3361 ggaactgccc gccaccggcc tgccccgcac ctaccgcgcc cgcacctatg gcgagatcac
     3421 ccag
//
LOCUS       DASUDV010000498         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1982970,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000498 DASUDV010000000
VERSION     DASUDV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982970"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFV30_10800"
     CDS             complement(<1..635)
                     /locus_tag="VFV30_10800"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4848622.1"
                     /translation="MNLGIGPTGSPEQVAALQQQLAANPGDRIARHNLAVELRKCGRL
                     DEALAQIERVWNEGLKLAETATMRGHLLADTGHFDEALEAYREAIRVKPELIEPHSTM
                     ASLLPQLGRGGEALDSLRQALLRAPHVGALWVEALSIARGHQAWDQLLDIAAQAEQRF
                     GTDTMISSFVANALTALGRDDEALARIDAALEQEPTYPPAHTIRAHILLRKG"
     gene            complement(632..2785)
                     /locus_tag="VFV30_10805"
     CDS             complement(632..2785)
                     /locus_tag="VFV30_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX4848623.1"
                     /translation="MPAAQPLTIERVFASPSLNGAVPRGVKLSPDGRYLTLLRNRADD
                     RERYDLWGFDHTTGQWTMLVDSLKLGSGKELSEAEKMQRERLRIGDLKGIVTYEWAAD
                     SKAILVPLDGDLYLAKLDGTVQRLSDTQEDELNPALSPKGGYVSFVRGRRLWTGKVGE
                     AAQPVTPEEPSETVYWGEAEFVAQEEMDRHTGYWWAPDDSRVAVQRFDEAQVAVVTRA
                     AIGAEGTKTFAQRYPLAGTPNAVVSLYVQKPDGSERVEVDLGPNKDIYLARVDWAPDG
                     KTLYVQRQDRGQTQLDMLAVDPATGKSRVLFSEAAAKGHWTDLSHGYRFLKDGSLVWW
                     SQRDGFGHLWHFKQGQWRQLTKGDWVVTALAGVDQASGKVFFTATKDDPLAHQVYAID
                     LKSPGKVTRLTELGFHNSASMEKSGKSLLISRSALNQPPQVYLADQAGKRIAWVEENK
                     LDAKHPYAPYLASHRPTEFGTIKAADGSVLHWNMITPPLEPGKRYPVFFSHYGGPSTQ
                     EVLKAWMNPLAQAIVAKGYIWFEIDNRGSDNRGVEFAKQIYRAMGSVEVEDQLAGAQY
                     LKSLPFVDGSKIATYGWSYGGYMTLKMLQANPGVYAAGISGAPVTKWELYDTHYTERY
                     MGDPKPDAAAYAKANAMADAGKIKDPLLLIHGMADDNVVFEHSSALIAKMQGQAVPFE
                     MMLYPGYTHRVGGPKVGVHLWQSIFAFLERHGVKP"
     gene            complement(2964..>3767)
                     /locus_tag="VFV30_10810"
     CDS             complement(2964..>3767)
                     /locus_tag="VFV30_10810"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX4848624.1"
                     /translation="TELQQTHAVAVEKQAKGEKLTKAEGEAVKEWQEKVDRINGKEPV
                     VEKLTKDEVKARSGSFMDYAGFLISTYILIVGKGSLLGGVAEAFSVMLLGIAAWKLGF
                     IQGQRSTRDYWIALIVAYGFGLTARYVGGLERMTFQPIPKTIWMTSELARIAVSVGHV
                     ALINLLMRGVIGRKIMWPFLAAGQMAFTLYLMQQLIGVHILYSPAGLHWPMAPGWAWL
                     AGLASAVIVFQLILANLWMRWFASGPLEWLWRSLAYCKKQPFRRLPAAE"
BASE COUNT          653 a         1282 c         1161 g          671 t
ORIGIN      
        1 cccttgcgca gcaggatgtg ggcccggatc gtatgagccg gcggataggt cggttcctgc
       61 tccagcgcgg cgtcgatcct ggcgagcgcc tcatcatctc ggcccagcgc agtcagcgcg
      121 ttggcgacaa acgagctgat catcgtatcc gtgccaaagc gctgctcggc ctgggcagcg
      181 atatccagca gctggtccca ggcctggtga ccgcgcgcga tcgacagggc ctcaacccac
      241 aacgcgccaa catggggcgc acgcagcagc gcctggcgca ggctgtcgag cgcctctccg
      301 ccccggccca attgcggcaa gaggctggcc atggtgctgt gcggctcgat cagttcgggc
      361 ttgacccgga tggcttcgcg ataggcctcc agcgcctcgt caaagtgacc ggtatcggcc
      421 agcaggtgtc cccgcatcgt cgcggtttcg gccagcttca acccttcgtt ccagacccgc
      481 tcgatctggg ccagtgcctc atccagtcgg ccgcatttgc gcagctcgac cgccaggttg
      541 tgccgcgcga tccggtcacc cggattggcc gcaagctgct gctgcagcgc tgcgacctgt
      601 tccggcgaac ccgtggggcc gataccaagg ttcatggctt caccccgtga cgttcgagga
      661 aggcgaagat cgattgccac aggtggacgc ccaccttcgg cccgccgacg cggtgggtat
      721 agccggggta gagcatcatt tcgaacggca ccgcctgccc ctgcattttc gcgatcaggg
      781 cagaggagtg ttcgaacacc acgttgtcat cggccatgcc gtggatcagc agcagcggat
      841 ccttgatctt gcccgcatcg gccatggcat tggccttggc ataggcggcg gcatccggtt
      901 taggatcgcc catgtagcgt tcggtgtaat gggtgtcgta aagctcccac ttggtcaccg
      961 gcgcgccgga aatcccggcg gcatagaccc ccggattggc ctggagcatc ttcagcgtca
     1021 tgtaaccgcc ataggaccag ccataggtcg cgatcttgct gccatccacg aagggcagcg
     1081 atttcaggta ctgcgccccg gccagctggt cctccacctc gaccgagccc atcgcgcggt
     1141 agatctgctt ggcgaattcg accccgcggt tgtccgagcc gcgattgtcg atctcaaacc
     1201 agatatagcc cttggcgaca atcgcctggg ccagcgggtt catccaggcc ttgagcactt
     1261 cctgcgtgct gggcccgcca tagtgcgaga agaacaccgg atagcgcttc cccggctcaa
     1321 gcggcggggt gatcatgttc cagtgcagca cgctgccgtc agctgccttg atcgtgccga
     1381 actcggtggg gcggtggctg gcgaggtacg gcgcgtaggg gtgcttggcg tcgagcttgt
     1441 tctcttccac ccaggcgatg cgcttgccgg cctggtccgc cagatagacc tgcggtggct
     1501 ggttcagcgc cgagcgcgag atcagcagcg acttgcctga cttctccatg ctggcactgt
     1561 tgtggaagcc cagttcggtc agccgcgtca ccttgcccgg gctcttcagg tcgattgcat
     1621 agacctgatg ggcgagcgga tcgtctttgg tcgcggtgaa gaacaccttg ccgctggcct
     1681 gatcgacccc ggcgagcgcg gtgacgaccc aatcaccctt ggtcagctgg cgccactggc
     1741 cctgcttgaa gtgccacagg tgcccaaaac catcgcgctg cgaccaccag accaggctgc
     1801 catccttgag gaagcgatag ccatgcgaca gatcggtcca gtggcccttg gccgcagcct
     1861 cgctgaacag cacgcggctc ttccctgtag cgggatcgac ggcgagcata tcgagctggg
     1921 tctggccgcg atcctgccgc tggacgtaca gcgtcttgcc atcgggtgcc caatcgaccc
     1981 gcgcgaggta gatgtccttg ttcggcccaa ggtccacctc aacccgctcg ctgccgtcgg
     2041 gcttctgcac atagagcgag accaccgcgt tgggcgtccc ggccagcgga tagcgctggg
     2101 caaaggtctt ggtgccctct gccccgatcg cggcgcgggt gacgacagcg acctgggcct
     2161 cgtcaaaccg ctgcacggcc acgcggctgt catcgggcgc ccaccaatag ccagtgtggc
     2221 ggtccatctc ttcctgagcg acgaattccg cctcgcccca gtagacggtt tccgaaggct
     2281 cttccggggt gaccggttgg gcagcctcgc ccaccttgcc ggtccacagc cgccgcccgc
     2341 gcacgaacga gacatagcca cccttcgggc tcagcgccgg gttgagctcg tcctcctgcg
     2401 tatcggacag gcgctggaca gtgccgtcca gcttggcgag gtaaaggtcg ccatccaacg
     2461 gcaccaggat cgccttgctg tcagccgccc attcataagt gacgatgccc ttcaagtcgc
     2521 cgatccgcag ccgctcgcgc tgcatcttct ctgcctcgga cagttccttg cccgaaccca
     2581 gcttcagcga atcgaccagc atggtccact gcccggtcgt gtggtcgaac ccccacaggt
     2641 cataccgctc gcggtcatcc gcccggttcc gcagcagggt cagatagcgg ccatcgggcg
     2701 aaagcttcac cccgcgcggc accgcgccat tcaggctggg gctggcgaag acgcgttcga
     2761 tggtcagcgg ctgggcggca ggcacggggc tctccgggga agtctgggca agaagcgggg
     2821 cggcaggagc aagcgcggca agcgcgcagg cggcaagcaa tcgggcaatc ggcgacacgg
     2881 caaactcacg cagcgaaaag ggcgccccgg caatccggga cgcccctctc taaccagctt
     2941 tgcggacagc gccagtggct ggatcactcc gccgccggca accgccggaa cggctgcttc
     3001 ttgcaatagg ccaggctgcg ccacagccat tccagcgggc cgctagcaaa ccagcgcatc
     3061 cacaggttgg cgaggatcag ctggaagacg atcaccgcgc tggcaaggcc ggccagccaa
     3121 gcccagcccg gcgccatcgg ccagtgcagc ccggccggcg aatagaggat atgcacgcca
     3181 atcagctgct gcatcagata gagcgtgaag gccatctgcc ccgctgccag gaacggccac
     3241 atgatcttgc gcccgatcac cccgcgcatc agcaggttga tcagcgcgac atggccgacc
     3301 gacaccgcga tccgcgccag ttcgctggtc atccagatcg tcttggggat cggctggaag
     3361 gtcatccttt ccagcccgcc gacgtagcgc gcggtcaggc caaagccgta ggcaacaatc
     3421 aacgcaatcc agtaatcgcg ggtcgagcgc tgcccctgga tgaagcccag tttccaggcg
     3481 gcgatcccca gcaacatcac gctgaacgcc tcggcaacgc cgcccagcag cgatcccttg
     3541 ccgacgatca ggatataggt cgagatcagg aacccggcat agtccatgaa cgagccggag
     3601 cgggccttga cttcatcctt ggtgagcttc tcgaccaccg gctccttgcc gttgatccta
     3661 tcgacctttt cctgccattc cttgaccgct tcgccttccg ccttggtcag cttctcgccc
     3721 ttggcctgct tctccaccgc aaccgcatga gtttgctgca attcggt
//
LOCUS       DASUDV010000499         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_856392,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000499 DASUDV010000000
VERSION     DASUDV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856392"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..439
                     /locus_tag="VFV30_10815"
     CDS             77..439
                     /locus_tag="VFV30_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4848625.1"
                     /translation="MKALFPNLVLLLVLATTACGPGEAPTKPLTPQQSAALKPADARL
                     AGLYEGSCKACHTVADSQAPLAGDRTMWDPRWTKGEDALLATVIQGKGAMPAGGQCFT
                     CTPEDLQALVRFMAGRDK"
     gene            436..1839
                     /locus_tag="VFV30_10820"
     CDS             436..1839
                     /locus_tag="VFV30_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX4848626.1"
                     /translation="MSTRRKVLLGGTGLVALGAAGLAGRRLWLDREPAPPPALNPQGR
                     TLWSNWSGLEHSYPAARAAPADEAELARLLATAQAPIRPVGAGHSFTALVPTEGTLLS
                     LDRISGLVNHDPAGLTATIKAGTRLAAIGPALAAIGQEMPNLPDINKQTLGGALATAT
                     HGTGKGIGALHAYVTRLRLVTPTGKVIDCSRHERPEIFQAARVGMGAFGVLSEVTLVN
                     RPLKRVRKTVTLVDTEELLGQWPELAGRHRNAEFLVLPFTGKSALIVHDETDEPVRPR
                     GPDQDAETLMQLKSLRDVFEFTPALRKQIAGRLLADIPAEVAVDQGWKLLSNERPVRF
                     KEMEYHLPIEAQVETLREVIRRIEGEVTDVFFPIEARIIASDDAWLSPFHGRESGSIA
                     VHAYYKEQHQWMFDQIEPVFREAGGRPHWGKLHSLGAKELTALYPMFAEAQAVRRSLD
                     PAGRMLNPFLTRLFENG"
     gene            1832..3094
                     /locus_tag="VFV30_10825"
     CDS             1832..3094
                     /locus_tag="VFV30_10825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX4848627.1"
                     /translation="MARLTRRMALFGTGALALAGIGVLAARPEELGAMHDPYFNTLAQ
                     ALKRAGLMRPTLVIDRTRLDANIAALRRSTDAAKLPLRIVAKSLPAPGLLERVRTGMA
                     SDRLMVFSAQMLLQLLDQNSRADWLMGKPLPAAEFARVASLAGPDAAGRVQWLIDTPQ
                     RLAEYAEVAKAGGFALKANLEIDVGLHRGGLIGGDAVTEVAKQAATLGVTVSGLMGYD
                     PHVPKMPSPDKAFAASQSAYREAIEALRAAGVADPSRLTLNTAGSPTVRRHCRDTVAN
                     EVSVGSAFVKPGDFDLDDLADLQPAAWIATPVIKASADQALPGVEALSGPMHWWDRNT
                     QRGFFIHGGHWLAKPASPPGLQYSPLFGRSSNQELLTGSNRIALKPDDIVFLRPDQSE
                     ALFLQFGDLAVFDGEAIAEFWPTLPISA"
     gene            complement(3182..>3433)
                     /locus_tag="VFV30_10830"
     CDS             complement(3182..>3433)
                     /locus_tag="VFV30_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4848628.1"
                     /translation="DTELDSLSSARAGLGAGQNRLESVVNNLTDNVTNLSDARSRIED
                     ADYSAETTAMAKAQILSQASTAMIAQANQSQQNVLSLLR"
BASE COUNT          594 a         1126 c         1123 g          590 t
ORIGIN      
        1 ctgctgggtt ggcttgcgga ttgcggtcac agtcactcct tgacagaaat atagttatag
       61 ctagattttt ggagtgatga aagccctttt ccccaacctc gtcctgctat tggtccttgc
      121 gaccaccgct tgcgggcccg gcgaagcgcc gaccaagccc ttgactccac agcaaagcgc
      181 cgcgctgaag cccgccgatg cgcggctggc cgggctttat gaaggctcgt gcaaggcctg
      241 ccacacggtg gccgattcgc aggccccgct cgccggagat cgcaccatgt gggatccgcg
      301 ctggaccaag ggcgaagatg ccctgctggc aacggtgatc cagggcaagg gcgcgatgcc
      361 cgcaggcggc cagtgtttca cctgcacacc cgaagatctg caagcgctgg tgcggttcat
      421 ggccgggagg gacaagtgag cacccggcgc aaggttctcc tgggcggcac cggccttgtc
      481 gcgctgggcg cggccgggct tgccggccgc cgcctgtggc ttgatcgcga gcccgccccg
      541 cccccggcgc tgaacccgca gggccgcaca ctgtggagca actggtcggg cctggaacac
      601 tcctatccag ccgcgcgggc cgcgccggcc gacgaggcgg aactggcccg cctgctcgcc
      661 accgcgcagg ctccgatccg cccggtcggt gccgggcaca gctttaccgc gctggttccg
      721 accgaaggca cccttctctc gctcgatcgg atcagcgggc ttgtgaacca cgatcctgcc
      781 ggacttaccg caacgatcaa ggccggaacc aggttggccg cgattggccc ggcgcttgcg
      841 gcgattggtc aggaaatgcc caatctgccc gatatcaaca agcagacgct tggcggcgca
      901 ctggccaccg caacgcacgg caccggcaag gggatcggcg cgcttcatgc ctatgtcacc
      961 cggctgcggc tggtcactcc caccggcaag gtgatcgact gcagccgcca tgaacggccc
     1021 gagatctttc aggcagcgcg ggtcgggatg ggcgcgttcg gcgtgctgag cgaagtcacg
     1081 ctcgtcaacc ggccgctcaa gcgggtcagg aagacggtca ccctggtcga taccgaagaa
     1141 ttgcttggcc agtggcccga gcttgccggg cggcaccgca acgccgaatt cctggtcctc
     1201 cccttcaccg gcaaatcagc gctgatcgtc cacgacgaaa ccgacgaacc ggtccgcccg
     1261 cgcggccccg atcaggatgc cgaaacgctg atgcagctca agtcgctgcg cgatgtgttc
     1321 gagttcacac cggcactgcg caagcagatc gccgggcggc tgctggccga tattccagcc
     1381 gaagtggcgg tcgatcaggg ctggaagctg ctgtcgaacg aacgcccggt gcgcttcaag
     1441 gaaatggaat accacctgcc gatcgaagcg caggtcgaaa cgctgcgcga ggtgatccgc
     1501 cggatcgaag gcgaagtgac cgatgtgttc tttccgatcg aggcgcggat catcgccagc
     1561 gacgatgcct ggctttcccc gttccacggc cgcgaaagcg gttcgatcgc ggtccatgcc
     1621 tattacaagg aacaacacca gtggatgttt gatcagatcg aaccggtgtt ccgtgaggcc
     1681 ggtgggcggc cccactgggg caagctgcat tcgcttggcg cgaaggagct cacggcgctc
     1741 tacccgatgt tcgccgaagc ccaggccgtg cgccgcagcc ttgacccggc gggccgcatg
     1801 ctcaatccgt tcctgaccag gctgttcgaa aatggctagg ctgacccgcc ggatggccct
     1861 gtttggcacc ggcgcactgg cgctggccgg gatcggtgtt ctggcggcaa gaccggagga
     1921 gctgggggct atgcacgatc cctatttcaa cacactggcc caagcgctca agcgtgccgg
     1981 gctgatgcgc cccaccctgg tgatcgatcg aacacggctt gatgccaata tcgcggcgct
     2041 gcgccgcagc accgatgcgg cgaaacttcc gctgcggatc gtcgccaaat cactccccgc
     2101 ccctggtctg ctcgagcggg tccgcaccgg aatggccagc gaccggctga tggtgttcag
     2161 cgcgcagatg ctgctgcaat tgctggacca gaactctcgt gccgactggc tgatgggcaa
     2221 gcccctgccc gcagccgaat tcgcgcgggt cgccagcctc gccgggcctg acgcagccgg
     2281 acgggtgcaa tggctgatcg atacaccgca gcgcttggcc gaatatgccg aagtggcaaa
     2341 ggccgggggt tttgccctca aggcaaacct ggaaatcgat gtcggcctgc accgcggcgg
     2401 gctgattgga ggcgacgccg tgaccgaagt ggcgaagcag gccgccacgc tgggcgtgac
     2461 cgtttccggc ctgatgggtt atgatccgca tgtccccaag atgcccagcc ccgacaaggc
     2521 cttcgccgca tcgcagagcg cctatcgcga ggcgatcgag gcgctgcgcg cggctggagt
     2581 ggccgaccct tcgcgtttga cgttgaacac cgcaggcagc ccaacagtgc gtcgccattg
     2641 ccgagatacg gtggcaaacg aggtttcggt gggttcggcc ttcgtgaagc cgggcgactt
     2701 cgatctggat gatctggccg atctccagcc cgccgcctgg atcgcgacgc cggtgatcaa
     2761 ggccagtgcc gatcaggccc tgcctggggt cgaagcgctt tcggggccga tgcactggtg
     2821 ggatcgcaac acccagcgcg gcttcttcat ccacggcggc cactggctgg ccaagcccgc
     2881 atcgccgccg ggcctgcagt attcgccgct gttcggccga tcgtccaacc aggaactgct
     2941 caccggatcg aaccgtatcg ccttgaagcc ggacgacatc gtcttcctgc gtcccgatca
     3001 aagcgaggcg ctgttcctgc aattcgggga tctggcggtg tttgatggcg aagcgatcgc
     3061 cgaattctgg ccgacgctgc cgatcagcgc ctgatcgatc ccgcacgcaa gaggcccggc
     3121 ggttcgggaa caaaccgccg ggccgactgc cttgaaggga caggcagatc aggtgtgccg
     3181 cttaacgcag cagcgagagg acgttctgct ggctctggtt ggcctgggcg atcatcgcgg
     3241 tcgaagcctg cgagaggatc tgggctttgg ccatcgccgt ggtttccgcc gaatagtccg
     3301 catcctcgat ccggctgcgc gcatcggaca ggttggtgac gttgtcggtc aggttgttga
     3361 ccaccgattc aagccggttc tgcccggcac cgagcccggc ccgcgcgctc gagagcgaat
     3421 cgagttcggt atc
//
LOCUS       DASUDV010000500         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1430799,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000500 DASUDV010000000
VERSION     DASUDV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430799"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFV30_10835"
     CDS             <1..759
                     /locus_tag="VFV30_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4848629.1"
                     /translation="TSAEREAFNDHPLTKAVREVARDADWPVTVRFFKEIADQAQSEA
                     DHVLVADLARELGRRDLGVILGQAAHADGHGVFRKTSFPLIPVPQGTNWTMVHAITRQ
                     ESQFAQNAMSHAGARGLMQLMPGTAREQAGKMALSFEPQALVGDAGYNLMLGDGYFRR
                     MLDYYGGSHPLAVAAYNAGPGNVNAWLRANGDPRNGGPDWVEWVEKIPISETRNYVQR
                     VLENAVVYDAMNPARAGYRGANPLSHYLGKRYPG"
     gene            756..1235
                     /locus_tag="VFV30_10840"
     CDS             756..1235
                     /locus_tag="VFV30_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX4848630.1"
                     /translation="MKPEGPPITPAGMAALRARYDHLLGTERPAIVEIVSWAAGNGDR
                     SENGDYLYGRKRMREIDRELAHLARRMKVARVIDPARQEDRARVWFGAEVTIADEADN
                     HRTVTLVGDDEQDASAGRIGWSAPLARALRGAAVGDLRRVVLPGGEKEWEVLQIVYP"
     gene            1299..2651
                     /locus_tag="VFV30_10845"
     CDS             1299..2651
                     /locus_tag="VFV30_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX4848631.1"
                     /translation="MAAATQEQQATIWGHPKGLVFLASTEMWERFSFYGMQALLTLYM
                     VKQLLLPGFAEQVWGLAALRSLLGGGEPISDTDLAGLIAGFYSGLVYFTPIVGGWIAD
                     RLLGAKRTVLIGVLLMSAGHLAMTFDQLFLVALVLLILGSGALKGNISAQVGTLYPPA
                     ARSMRDRGFSIFSTGINIGAVSGPLVTGAVAAIWGWHAGFAVAAALMLVALVTYLAGQ
                     RHFPEAPPRRADPEAKPLTKSEKRKVWALIVVIALTMPAEITYPMIWTIGPLWIDKYV
                     ALGNVPAPWFGSADSFGSILAAAPLVALWAAQARKGQEPTSVGKIAIGTGLTGIGAMI
                     LAAGSATQTGPDTINILWPILGYFTMGLAWMYYWPTTLSLVSRVAPPAVAATMVGGAF
                     FSPFVAHTVAGMIGTRFDNMDPAAFWAMDGAIGLAGAVLLFALKNPINRVIEARAEAD
                     "
     gene            2651..>4057
                     /locus_tag="VFV30_10850"
     CDS             2651..>4057
                     /locus_tag="VFV30_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4848632.1"
                     /translation="MRRLIASVLLAALPLASAMAEVAPVSTAPTAAPIQAADFARIPY
                     VSQPELSPDGLYLGGLMGIEGRQVIAIVSLFDQAKPVVLALPEDNQAAWVRWVNNDHL
                     IVGIRALMPVVGDRWIVSRMISVHRPTGKVTKVLWDQNGQNSADVLWTARDGSPQILV
                     AAQNSIFVDEEFWPKVWRVNVETGKRVVAQKGRDGVMDWYADAAGRVRAGLWYEDHGR
                     KFRLLYRAADEGTYRTVDKADSKKEEDLLSPFLFLPGTNNALVVHDDESGRSAIYEVD
                     MTTRQDLRTVFSAPNGAEVSGVEVAADGTSLLGVYYTGKSDGMTWLDPKLAELQTLID
                     KALGTSGRRAQVVSLSADRTRLLVRVDRADSPGALYYFNTADGAMQRIAFYNEALGTK
                     PLNPVRTIQYKARDGLEIEAVLTLPKDRPAKALPIVVMPHGGPWAHDTADYDYWAQFV
                     ASRGYAVIQPNFRGSTGYG"
BASE COUNT          666 a         1310 c         1384 g          697 t
ORIGIN      
        1 accagcgccg agcgcgaggc tttcaacgat catccgttga ccaaggcggt gcgcgaagtg
       61 gcgcgcgatg ccgactggcc ggtgaccgtg cgcttcttca aggaaatcgc cgaccaggcg
      121 cagagcgagg ccgatcacgt gctggtcgcc gatctggcgc gcgaactggg caggcgcgat
      181 ctgggggtga tcctgggtca ggcggcccat gccgatggcc acggcgtatt ccgcaagacc
      241 agtttcccgc tgatcccggt gccccagggc accaactgga ccatggttca cgccattacc
      301 cgccaggaaa gccagtttgc ccagaacgcg atgagccacg ccggtgcgcg cggcctgatg
      361 cagctgatgc ccggcaccgc gcgcgagcag gccggcaaga tggcgctgag ctttgaaccg
      421 caggcgctgg taggcgatgc cggctacaac ctgatgctgg gcgatggcta tttccgccgg
      481 atgcttgatt actacggcgg cagccacccg ctggcggttg ccgcctacaa cgcggggccc
      541 ggcaatgtga acgcctggct gcgcgccaac ggcgatccgc gcaatggcgg gcccgactgg
      601 gtcgaatggg tcgagaagat cccgatttcg gaaacccgca actatgtcca gcgggtgctg
      661 gaaaacgcgg tagtctatga tgcgatgaac ccggccaggg ccggctatcg cggcgccaat
      721 ccgctcagcc actatctggg caagcgttac cccggatgaa acccgaaggt ccgccgatca
      781 ccccggccgg catggccgcg ctcagggcac gctatgatca cctgctgggc accgaacgcc
      841 ccgcgatcgt cgagatcgtg tcgtgggcgg caggcaatgg cgatcgcagc gaaaacggcg
      901 actatcttta tggccgcaag cgcatgcgcg agatcgaccg cgaactggcg cacctggcgc
      961 ggcggatgaa ggtggcccgc gtgatcgatc cggcccggca ggaagatcgg gcgcgggtct
     1021 ggttcggcgc cgaagtgacc atcgccgacg aggccgacaa ccaccgcacg gtcaccctgg
     1081 tgggcgacga cgaacaggac gcatcagccg ggcggatcgg ctggagcgcg ccgctggccc
     1141 gcgcgctgcg cggcgcggcg gtgggcgatc tgcgccgcgt ggtcctgccc ggcggcgaga
     1201 aggaatggga agtattgcag atcgtctatc cctgagccag cttgcaagtg cttggtttgg
     1261 ctctatcgtt gcagccagaa cgcattgggg agagcgggat ggcggcggca actcaggaac
     1321 agcaggcaac catctggggg caccccaagg gtctggtgtt ccttgcatcg accgaaatgt
     1381 gggaacgctt ctcgttctac gggatgcagg ccctgctcac gctctacatg gtcaagcaac
     1441 tgctgctgcc cggattcgcc gaacaggtct gggggctggc cgcgctgcgc agcctgctgg
     1501 gcggcggcga gccgatttcg gataccgatc ttgccggcct gatcgcgggc ttctattcgg
     1561 ggctggtcta tttcaccccg atcgtcggcg gctggattgc cgaccggctg ctgggcgcca
     1621 agcgcaccgt gctgatcggc gtgctgctga tgagcgcggg gcacctggcc atgaccttcg
     1681 accagctgtt cctggttgca ctggtgctgc tgatcctggg ttcgggcgcg ctcaagggta
     1741 acatttcggc ccaggtcggt acgctctatc cgcccgcggc gcgctcgatg cgcgatcgcg
     1801 gtttctcgat cttctcgacc gggatcaata tcggcgcggt ttccggcccg ctggtgaccg
     1861 gggcggtggc ggcgatctgg ggctggcacg ccggctttgc cgtggccgcc gccttgatgc
     1921 tggtggcttt ggtcacctac cttgccggtc agcgccattt ccccgaagcc ccgccccgcc
     1981 gcgccgatcc cgaagccaaa ccgctgacca agtctgaaaa gcgcaaggtc tgggcgctga
     2041 tcgtggtgat cgcgctgacc atgccggccg aaatcaccta tccgatgatc tggacgatcg
     2101 gcccgctgtg gatcgataag tacgtggcgc tgggcaatgt gccagcgccc tggttcggct
     2161 cggcggattc gttcggctcg atcctggccg cggcgccgct ggtggcgctg tgggccgccc
     2221 aggcgcgcaa gggccaggag ccgaccagcg tcggcaagat cgcgatcggc accggtctta
     2281 ccgggatagg cgcgatgatc cttgcggcag gcagcgctac ccagaccggg cccgatacga
     2341 tcaacattct ctggccgatc ctgggctatt tcaccatggg gctggcctgg atgtattact
     2401 ggccgaccac gctttcgctg gtcagccggg tggccccgcc ggcggtggcg gctaccatgg
     2461 tgggtggcgc ctttttctcg cccttcgtcg cccataccgt ggccggcatg atcggcaccc
     2521 ggttcgacaa tatggatccg gccgcgttct gggcgatgga cggcgcgatc ggcctggctg
     2581 gcgcggtgct gctgttcgcg ctgaagaacc cgatcaaccg ggtgatcgaa gccagggctg
     2641 aggccgactg atgaggcggc tgatcgcgtc ggtcctgctc gcggcactgc cgctcgcctc
     2701 ggcaatggct gaggtcgcgc ccgtttccac cgcacccacc gcggcgccga tccaggcggc
     2761 cgattttgcc cgcattccct atgtctccca gcccgaactt tcccctgatg gcctctatct
     2821 gggcgggctg atggggatcg aaggccgcca ggtgatcgcg atcgtcagcc tgttcgacca
     2881 ggccaagccg gtggtcctgg ccctgcccga agacaaccag gcagcctggg tgcgctgggt
     2941 caacaacgat catctgatcg tcgggatccg ggcgctgatg ccggttgtgg gcgatcgctg
     3001 gatcgtctcg cggatgattt cggtccaccg gccgaccggc aaggtcacca aggtgctgtg
     3061 ggaccagaac ggccagaaca gcgccgacgt gctgtggacc gcccgggacg gttccccgca
     3121 gattctcgtc gccgcgcaga attcgatctt tgtcgacgag gaattctggc ccaaggtctg
     3181 gcgcgtcaat gtcgagaccg gcaagcgagt ggtggcccag aagggccgcg acggagtgat
     3241 ggactggtac gccgacgccg ccggccgggt ccgtgccggg ctgtggtacg aggatcatgg
     3301 gcgaaagttc cgcctgctct atcgcgcggc ggacgaaggc acctaccgca cggtcgacaa
     3361 ggcggactcc aagaaggagg aagatctcct ttctccgttc ctgttcctgc cgggcaccaa
     3421 caacgccctg gtggtgcatg acgatgaaag cggccgctcg gcgatctacg aagtcgacat
     3481 gaccacccgc caggacctgc gcacggtgtt ctccgcgccc aatggtgcgg aggtcagcgg
     3541 agtcgaggtg gcggccgatg gcaccagcct gcttggcgtc tactacaccg gcaagagcga
     3601 cgggatgacc tggctcgatc ccaaactggc cgaattgcag accctgatcg acaaggcgct
     3661 gggcacctcg ggccggcggg cgcaggtggt gtcgctgtcg gccgatcgca cccggctgct
     3721 ggtccgggtc gaccgggcgg attcgcccgg cgcgctctat tacttcaaca ccgcagacgg
     3781 ggcgatgcag cggatcgcct tctacaacga agcgctgggc accaaaccgc tcaatccggt
     3841 gcggacgatc cagtacaagg cccgtgacgg actggagatc gaggccgtgc tgaccctgcc
     3901 aaaggatcgc cccgccaagg ccctgccgat cgtggtcatg ccgcacggcg ggccctgggc
     3961 gcatgatacc gccgattacg attactgggc acagttcgtc gccagccgcg gctatgcggt
     4021 gatccagccc aacttccgcg gctccaccgg ctacggc
//
LOCUS       DASUDV010000501         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_105329,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000501 DASUDV010000000
VERSION     DASUDV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105329"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFV30_10855"
     CDS             <1..286
                     /locus_tag="VFV30_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4848633.1"
                     /translation="VVSHTPIEIEALWRLRDAHGWVDLGRKHIPAPERNWTWWSYRTF
                     WQEKDRGRRLDHMWASPQLAAQSASHRFVEETRRWDQPSDHVPLITEFAL"
     gene            283..1335
                     /gene="ribA"
                     /locus_tag="VFV30_10860"
     CDS             283..1335
                     /gene="ribA"
                     /locus_tag="VFV30_10860"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX4848634.1"
                     /translation="MTSPARRIAQALDALRHGWPVALDGAFTLLPAETGLGPAGARTM
                     LISAARAVTLKLANQREAAAPEAPVLIRGAEPFDLAAARALADPALDLAYPLKGPFLA
                     EPLVHGDAARAAMELARLAGILPAFLVDPDPAGEAVALSASDLTAWKDTAQLAIAARA
                     RLPVAAAEEAEIVAFRSADDLREHVALVIGKQTSDRAPLVRLHSECLTGDILGSLKCD
                     CGPQLDAALREMAREAEAGGWGVLLYLRQEGRGIGLINKLRAYELQDQGFDTVDANNR
                     LGLPTEARDFPVAARMLDLLGVPAIRLMTNNPAKVKALSAAGVTVTERVPHQLPPNPH
                     NARYLDTKRDRTGHIL"
     gene            1332..1838
                     /locus_tag="VFV30_10865"
     CDS             1332..1838
                     /locus_tag="VFV30_10865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX4848635.1"
                     /translation="MTGWTIRPEQPGDEAAIHDLVARAFAPMPFSEGDEQDLVDALRA
                     AGDLILSLVAVDGAGPIIGHVSFSPVTVEDAACGWFQMAPVSVSPEVQRSGIGSALIE
                     AGIARLREAGARGIAVVGNPVYYERFGFAVVPGLAPLSAHDAPYFRAMALQGPAPQGT
                     LRYAPAFG"
     gene            complement(1835..>2080)
                     /locus_tag="VFV30_10870"
     CDS             complement(1835..>2080)
                     /locus_tag="VFV30_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX4848636.1"
                     /translation="TGRIAGNWFVQGQSKENYSAFITVAPEGRVNASAGLAIEGNDAR
                     FAPGVSRSSVFVASWTDEPLAIGANTLGPGLKRTERR"
BASE COUNT          289 a          736 c          741 g          314 t
ORIGIN      
        1 ggtggtcagc cacaccccca tcgagatcga agcgctgtgg cggctgcgcg atgcccatgg
       61 ctgggtcgat ctgggccgca agcatattcc cgcgccggaa cgcaactgga cctggtggtc
      121 ctatcgcacc ttctggcagg agaaagaccg cgggcggcgg ctcgatcaca tgtgggcctc
      181 gccccagctg gcggcgcaat cggccagcca ccgctttgtc gaggaaaccc gccgctggga
      241 ccagcccagc gatcacgtgc cgctgatcac ggagtttgcc ctgtgaccag cccggcgcgc
      301 cggatcgcgc aagcgctcga cgcgctgcgc cacggctggc cggttgccct ggacggggcc
      361 ttcacgctac tgccggcgga aaccgggctg ggcccggcgg gcgcgcggac catgctgatc
      421 tcggccgcgc gggcggtgac cctgaagctg gccaaccagc gcgaggccgc cgcgcccgag
      481 gccccggtgc tgatccgcgg cgccgaacct ttcgatctgg ccgccgcgcg ggcgctggct
      541 gacccagcgc tcgatctggc ctatccgctc aaggggccgt tcctggccga gccgctggtc
      601 cacggcgatg ccgcccgggc ggcgatggaa ctggcgcgcc tggccgggat cctgccggcg
      661 ttcctggtcg atcccgatcc ggcgggcgag gcggtggcgc tttccgccag cgacctcacc
      721 gcgtggaagg acaccgcgca gcttgccatc gcggcccgcg cccggcttcc ggtcgccgcg
      781 gccgaagagg cggagatcgt ggccttccgt tcggccgacg acctgcgcga gcatgtcgcg
      841 ctggtgatcg gcaagcagac gagcgaccgc gccccgctgg tccggctcca ttccgaatgc
      901 ctgaccggcg atatcctggg cagcctcaaa tgcgattgcg ggccgcaact ggacgccgcg
      961 ctgcgcgaaa tggcgcgcga ggccgaagcc ggcggctggg gcgtgctgct ctacctgcgc
     1021 caggaagggc gcgggattgg cctgatcaac aagctgcgcg cctatgagct gcaagaccag
     1081 gggttcgaca cggtcgatgc caacaaccgc ctgggcctgc cgaccgaggc gcgcgatttt
     1141 ccggtggcgg cgcggatgct cgatctgctg ggcgtgccgg cgatccggct gatgaccaac
     1201 aacccggcca aggtcaaggc gctgtccgcc gccggggtga ccgtgaccga acgggtgccg
     1261 caccagctgc cgcccaaccc gcacaacgcc cgctacctcg ataccaagcg cgaccgcacc
     1321 gggcacatcc tgtgaccggc tggacgatcc gtcccgaaca gcccggcgac gaggccgcga
     1381 tccacgatct ggtggcccgc gcctttgcgc cgatgccgtt cagcgagggc gacgagcagg
     1441 atctggtcga tgccttgcgc gcggctggcg atctgatcct ctcgctggtc gcggtggacg
     1501 gcgcgggccc gatcatcggg cacgtatcgt tcagcccggt cacggtggag gatgcggcct
     1561 gcggctggtt ccagatggcc ccggtttcgg tctcgcccga ggtgcagcgc agcgggatcg
     1621 gctcggcact gatcgaggcc gggattgcgc ggctgcgcga agccggcgcg cgcggcatcg
     1681 cggtggttgg caatccggtc tattacgagc gcttcggctt tgccgtcgtg cccggtctgg
     1741 ccccgctctc ggcccacgac gcgccctatt tccgggccat ggcgctgcaa gggccagccc
     1801 cgcagggcac gctgcgttac gctccggcct tcggttagcg gcgttcggtc cgcttgaggc
     1861 ccgggcccag cgtgttggcg ccgatcgcca gcggctcgtc ggtccagctc gccacgaaca
     1921 cgctggagcg cgagacaccc ggagcgaagc gcgcgtcgtt gccttcgatc gccagcccgg
     1981 ccgaggcatt gacgcggcct tcgggggcga cggtgatgaa ggcggaatag ttctctttcg
     2041 actggccctg gacgaaccag ttgccggcga tccgcccggt
//
LOCUS       DASUDV010000502         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2159821,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000502 DASUDV010000000
VERSION     DASUDV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159821"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..529
                     /locus_tag="VFV30_10875"
     CDS             65..529
                     /locus_tag="VFV30_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848637.1"
                     /translation="MGEYTIREDDLTGAAIAALLRLHLDEMHQWSPPESVHAMPIERL
                     RAADVTFFSAWAGERLAACGAIKQLDPGHGELKSMRADPAFRGRGAGKAVLLHLMAVA
                     RERGYARLSLETGRPEQFLPARRLYESHGFAECPPFGDYTADPFSICMTRTL"
     gene            526..1056
                     /locus_tag="VFV30_10880"
     CDS             526..1056
                     /locus_tag="VFV30_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848638.1"
                     /translation="MILRDATPADIPALSRLAVDSFVAKFGPLYSAQDLATFLEESLS
                     EAAVAADMANPDRLYQLAEGEGGALMGFCKIGLICGFPDHARGSRVMELKQLYTAPEA
                     TGQGIGGALMDWAMDQFAARGADEVQISVYSENFGAHRFYARYGFEKVADITFKVGEQ
                     LDPEFLFAQMIGPNPA"
     gene            1091..2377
                     /locus_tag="VFV30_10885"
     CDS             1091..2377
                     /locus_tag="VFV30_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4848639.1"
                     /translation="MLQPSSPAATPDLRPLFPSAVSDPATSPIDRILDSVRSHLGMEI
                     AFASRIEGGLRRFTHIRADCPVPVAPGDAEVLEETLCYLVLQGELPQLMHDARTYPAA
                     LPIGITLGLPVASHLNVPLRLSTGELYGTFCCVNRTPDHSLTERDMATLRAFADLASQ
                     LIEADLVTQERDSAVAARIQSALSDDTIRIVHQPIHKLVGGQPVGVECLSRFAGPEPR
                     SPDLWFKDAAAVGLGVEFELAAVRAALRTLGYVPEPHYLSINVSPATVLSGQLADLIS
                     GHSPARLVIEITEHDAVDDFAGLGRALDSLRPFARIAIDDVGAGYAGLRHIVDLRPDI
                     LKLDMSLTRDIEADPARRALAHALVRFAAEMQCTIVAEGVETEAERDTLVKLQVPLAQ
                     GYLYNKPMPVVAAQQVLLGVARAETPAPDLAQRRRA"
     gene            2436..>3040
                     /locus_tag="VFV30_10890"
     CDS             2436..>3040
                     /locus_tag="VFV30_10890"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX4848640.1"
                     /translation="MSSIRPWRDIARRTSRQIMVGSVPVGGDAPITVQTMTNTLTSDA
                     VATIDQIRRCEEAGADIIRVSCPDVESTAAFGKIVRAARIPIVADIHFHYKRALEAAD
                     AGAACLRINPGNIGSSERVAEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPCPEAL
                     VESALDHIKLLQDHDFHEFKVAVKASDVFLAVAAYMGLAEAV"
BASE COUNT          502 a         1030 c          998 g          510 t
ORIGIN      
        1 aatccggcgg caatccggcg cagcgtgctt gcaatcatgg ggcagggcat agcagaaggc
       61 cgccatgggg gaatacacga tccgcgaaga cgacctgacc ggggcagcga tcgccgcgct
      121 gctgcggctg catctggacg aaatgcacca gtggtcgccg cccgaaagcg tccacgccat
      181 gccgatcgag cggctgcgcg cggccgacgt caccttcttt tccgcctggg cgggggagcg
      241 gctggccgcc tgcggggcga tcaagcagct cgatcccggc cacggcgagc tgaagtcgat
      301 gcgggccgat ccggcctttc gcgggcgcgg tgcgggcaag gcggtcctgc tccacctgat
      361 ggccgtggcg cgggagcggg gttacgcccg gctcagcctg gaaaccggcc ggcccgaaca
      421 gttcctgccg gcccggcggc tttacgaaag ccacggtttt gccgaatgcc cgccgttcgg
      481 tgattacacc gccgatccct tttcgatctg catgacgagg accctgtgat cctgcgcgac
      541 gccacgcccg ccgacattcc ggccctctcc cgcctcgccg tagacagctt cgtcgccaag
      601 ttcggcccgc tctacagcgc ccaggatctc gccaccttcc tggaggagag cctgtccgaa
      661 gccgccgtgg cagccgacat ggccaatccc gaccggctct atcagctggc cgaaggcgaa
      721 ggcggcgcgc tcatggggtt ctgcaagatc ggcctcatct gcgggtttcc ggaccacgcc
      781 cggggcagcc gggtgatgga gttgaagcag ctctacaccg cgcccgaggc caccgggcag
      841 gggatcggcg gcgcgctgat ggactgggcg atggaccagt tcgcggcgcg cggggcggac
      901 gaggtgcaaa tctcagtcta ttccgaaaac ttcggcgcgc accgtttcta cgcgcggtat
      961 ggatttgaga aggtcgccga catcactttc aaggttggtg agcagctgga cccggaattc
     1021 ctgttcgcgc agatgattgg acctaatccg gcttaaccat cccttagagt ccgtgcggca
     1081 ggatgcggtc atgctccagc cgtcctcccc tgccgccacg cccgatctgc gtcccttatt
     1141 cccaagcgca gtcagcgatc cggcaacgtc cccgattgac cgtatccttg attcggtgcg
     1201 cagccacctc ggcatggaaa tcgctttcgc ctcgcggatc gaaggcggac tgcggcggtt
     1261 tacccatatt cgggccgatt gccccgtgcc ggtcgctccc ggtgatgccg aagtgctgga
     1321 ggagacgctg tgctatctgg tgctgcaagg cgaattgccg cagctgatgc acgatgcgcg
     1381 cacctacccg gccgcgctgc caattggtat cacgctgggg ctgccggtcg cctcgcacct
     1441 caacgtgccg ctgcggctca gcaccgggga gctttacggc acgttttgct gcgtcaaccg
     1501 cacccccgat cacagcctga ccgagcgcga catggcgacc ctgcgggctt ttgccgatct
     1561 ggccagccaa ctgatcgaag ccgatctggt gacgcaggaa cgcgattcgg cggtggctgc
     1621 gcggatccaa tcggccctca gcgatgacac catccggatc gtgcaccagc cgatccacaa
     1681 gctggtcggc ggccagccgg tcggcgtcga atgcctgtcc cggttcgccg gaccggagcc
     1741 gcgcagcccg gatctgtggt tcaaggacgc agccgcggtt ggcctgggcg tggaattcga
     1801 actggcggcg gtgcgcgcgg cgctgcgcac tctaggctac gtgcccgaac cgcattatct
     1861 ctcgatcaat gtctctccgg ccacggtcct gtccgggcag ctggccgatc tgatcagcgg
     1921 ccattcgccc gcccggctgg tgatcgaaat caccgagcac gatgcggtcg acgattttgc
     1981 cgggctgggc agggcgctgg acagcctgcg gcccttcgcc cggatcgcga tcgacgatgt
     2041 cggtgccggc tatgccgggc tgcgtcacat tgtcgatctg cgccccgata tcctcaagct
     2101 ggacatgagc ctgacgcgcg atatcgaggc ggacccggcc cgacgtgccc tggcccatgc
     2161 cctggtccgc tttgcggcgg aaatgcaatg cacgatcgtc gcggaagggg tggagaccga
     2221 ggccgagcgc gacacgctgg tgaagctgca ggtaccgctg gcccaaggct atctctacaa
     2281 caagccgatg ccggtggttg ccgcgcagca ggtcctgttg ggggtggccc gtgctgaaac
     2341 gccagcaccg gaccttgccc agcgccgccg ggcctgaagc accctgcacg tttagaccat
     2401 ttgcggcgcg ggccgcgctt tcctatatgc gccccatgtc ctcgatcaga ccctggcgcg
     2461 atatcgcgcg gcgcacgtcc cggcagatca tggtgggctc tgtcccggtg ggcggcgacg
     2521 cgccgatcac ggtccagacc atgaccaaca ccctgacctc tgacgcggta gcgacgatcg
     2581 accagatccg ccgctgcgaa gaggccgggg ccgacatcat ccgcgtctcc tgcccggacg
     2641 tggagagcac cgcggccttc ggcaagatcg tccgcgccgc gcgcatcccg atcgtcgccg
     2701 acatccattt ccattacaaa cgcgcgctcg aggcggccga cgccggcgcg gcgtgcctgc
     2761 ggatcaatcc gggcaacatc ggcagcagcg agcgcgtcgc cgaagtggtc cgcgcggcca
     2821 aggcgaacgg ctgcgcgatc cggatcgggg tcaacgccgg cagcctcgag aaggacctgc
     2881 tcgaaaagta cggcgagccg tgccccgagg cgctagtcga aagcgcgctc gaccacatca
     2941 aactgctgca ggaccacgac ttccacgagt tcaaggtcgc ggtaaaggcg agcgacgtgt
     3001 tcctggccgt tgccgcttac atgggtctgg ccgaagcggt
//
LOCUS       DASUDV010000503         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1430938,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000503 DASUDV010000000
VERSION     DASUDV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430938"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1890
                     /locus_tag="VFV30_10895"
     CDS             <1..1890
                     /locus_tag="VFV30_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX4848641.1"
                     /translation="FDFSDSESELVQLRDEASRAKADFAALVGLIEAAPMPMWFRGPD
                     GALQLVNQAYVDAVAGQDARQVAEDGIELIESVDGLTARQVALQAAAKQLPIERMVSV
                     TIDGQRRALRVSDLPLGQEGVAGYAVDVEEMEELSRALRAFRDAQRTMLDQLSAGVAQ
                     FDAKRQLTFANPPFQRIFALKSTVLLDPPPFDRLLDMARDAGRVPEARDFPAWRRERA
                     GWFTAGSAQEEAWMLADGTHLRIVAQPLPDGGLILIAEDRTEQLQLSAARDTLLRTRT
                     ATFDSLFESVAVFAPDGRLQLWNRRFAADWGLEDAFLDQHPRIESLLEQIAPRLARPI
                     KVKAVGDVVRAATLDRKQTGSRAVLADGRTLEFAGVPLPDGNGLLTVLDITDSQKAEE
                     ALKQRAAALEEADALKTRFLANMSYEFRTPLTSIGGFAELLQAGLGGELSESGKDYVA
                     AILQSVERLGEQIESVLDLSQSEAGMLPLAHEPIELLPFVTRLVEDRAERIRTAGLTL
                     DLRGDKSVGRIEGDKRRLGRAIGHLIDNAVSATPSGGRVLVELGAHKGKARIVISDNG
                     AGMDPATLARALEGIKVSADGKGVERRQGLGLPLARQLIEAHGGTLELLSEPGQGTAA
                     IVELP"
     gene            1887..2330
                     /gene="tsaE"
                     /locus_tag="VFV30_10900"
     CDS             1887..2330
                     /gene="tsaE"
                     /locus_tag="VFV30_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX4848642.1"
                     /translation="MTVDLPDLAAMDAFGARIAAALRPGDVVALSGGLGAGKTTLARA
                     IIAALGHAGEVPSPSFAIIETYDPPSVRLPLVHADFYRLKHPEEAEELGLDDYRQGAA
                     LLAEWPEMAGGFAHEPGCLSITLETAETGRIAIVKPGDDWLGRLG"
     gene            2327..>3292
                     /locus_tag="VFV30_10905"
     CDS             2327..>3292
                     /locus_tag="VFV30_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4848643.1"
                     /translation="MSDDLPANLAPFLAAAGWGEAAIEPLAGDASFRRYFRLRRASGK
                     TAMLMDAPPPHEDPQPFLRAAKWLDANGLRAPRILAEDAERGLVLLEDFGEVRMREYL
                     DSWPADERDVYRAAIDTLVELHRLPAGPFLDYSMSEYIREAKLLTDWFCPAQNLYIDG
                     PGYVAAWEKVLGDMLPRQRPGVAVLRDYHAENIMLLGSLAEQGLLDFQDALNGHPAYD
                     LVSLLQDARRDVAQELEAEMFDYYVEKTGAGADFLADYARLGAQRNAKIVGIFVRLWK
                     RDGKPRYLDFIPRVWAYLERDLSHPALAPVAAWFDANIPAELRDAG"
BASE COUNT          491 a         1120 c         1174 g          507 t
ORIGIN      
        1 ttcgactttt ccgacagcga gagcgagctg gtgcagctgc gcgacgaagc ctcgcgggcc
       61 aaggccgatt tcgccgcgct ggtcggcctg atcgaagccg cgccgatgcc gatgtggttc
      121 cgcgggccgg acggggcgct gcaactggtc aaccaggctt atgtcgatgc cgtggccggc
      181 caggacgcgc gccaggtggc cgaagacggg atcgagctga tcgaaagcgt cgacggactg
      241 accgcccgcc aggtcgcgct gcaggctgcg gccaagcagc tgccgattga gcggatggtc
      301 tcggtcacga tcgacggcca gcgccgcgcg ctgcgggttt cggacctgcc gctggggcag
      361 gaaggcgtgg ccggctatgc cgtggacgtg gaagagatgg aggagctgtc gcgcgcgctc
      421 cgcgccttcc gcgatgccca gcggacgatg ctcgatcagt tgtcggccgg ggtcgcccag
      481 ttcgatgcca agcgccagct gaccttcgcc aacccgccgt tccagcggat cttcgcgctg
      541 aaaagcacgg tcctgctcga tccgccgccg tttgaccgcc tgctcgacat ggcgcgcgac
      601 gcgggccgcg tgcccgaggc gcgcgatttc ccggcctggc ggcgcgagcg ggccggctgg
      661 ttcaccgccg gatcggccca ggaagaggcc tggatgctgg ccgacggcac ccatctgcgc
      721 attgtcgccc agccgctgcc cgatggcggc ctgatcctga ttgccgagga ccggaccgag
      781 cagctgcagc tgtccgccgc gcgcgacact ctgctgcgca cccgcaccgc cacctttgac
      841 agcctgttcg aatccgttgc ggtctttgcc cccgatggcc ggctgcagct gtggaaccgc
      901 cggttcgcgg ccgattgggg gctggaggac gccttcctcg atcagcaccc ccggatcgaa
      961 agcctgctgg agcagatcgc cccgcgcctg gcccggccga tcaaggtcaa ggcggtcggc
     1021 gatgtcgtgc gcgccgccac gctggaccgc aagcagaccg gcagccgcgc ggtgctggcc
     1081 gatggccgga cgctggaatt cgccggagtg ccgctgcccg atggcaacgg cctcctgacc
     1141 gtgctcgaca tcaccgacag ccagaaggcg gaggaagcgc tgaagcagcg cgccgccgcg
     1201 ctggaagagg ccgatgccct caagacccgc ttcctcgcca acatgagcta tgagttccgt
     1261 acgccgctca cctcgatcgg cggctttgcc gaactgcttc aggccgggct gggcggcgag
     1321 ctgagcgaga gcggcaagga ctatgtcgcg gcgatcctgc aatcggtcga gcggctgggc
     1381 gagcagatcg aaagcgtgct cgacctctcg caaagcgagg ccgggatgct gccgctggcg
     1441 cacgagccga tcgagctgct gcccttcgtg acccgcctgg tcgaggaccg ggccgagcgg
     1501 atccggaccg ccgggctgac cctggacctg cgcggcgaca agagcgtcgg gcggatcgag
     1561 ggcgacaagc gccggctggg ccgcgcgatc ggccacctga tcgacaacgc ggtttccgcc
     1621 accccttcgg gcgggcgggt gctggtggaa ctgggcgccc acaagggcaa ggcccggatc
     1681 gtcatttccg ataacggcgc ggggatggac ccggccaccc tggcgcgcgc gctggaaggc
     1741 atcaaggtca gcgccgatgg caagggcgtg gagcggcggc aagggctggg tcttccgctg
     1801 gcacgccagc tgatcgaggc ccacggcggc acgctggagc tgctgtccga accgggccag
     1861 ggcaccgcgg cgatcgtcga gctgccgtga cggtcgacct tcccgatctc gccgcgatgg
     1921 acgccttcgg cgcgcggatt gccgcggcgc tgcgccccgg cgatgtggtg gcgctgtcgg
     1981 gcggtctggg cgcgggcaag accacgctgg cccgcgcgat catcgccgcc ttgggccatg
     2041 ccggcgaagt gccgtcgccc agctttgcga tcatcgaaac ctacgatccg cccagcgtcc
     2101 ggctgccgct ggtccatgcc gatttctacc gcctgaagca tccggaagag gccgaggagc
     2161 tggggctgga cgattaccgc cagggcgcgg cgctgcttgc cgaatggccg gaaatggccg
     2221 gcggtttcgc gcacgagccg ggctgtcttt cgatcacgct ggaaactgcg gaaacggggc
     2281 ggattgcgat tgtaaagccg ggggatgatt ggctagggcg gctgggatga gcgacgatct
     2341 tcccgccaac ctcgccccgt tccttgccgc cgccgggtgg ggggaagccg cgatcgaacc
     2401 gctggccggg gacgcgtcgt tccggcgcta tttccgcctg cgccgggcca gcggcaagac
     2461 cgcgatgctg atggacgcgc ccccgccgca cgaggatccg cagccgttcc tgcgcgccgc
     2521 caagtggctc gatgccaacg gcctgcgcgc gccccggatc ctggccgagg atgcggagcg
     2581 cgggctggtc ctgctggagg atttcggcga ggtccggatg cgcgagtatc tcgacagctg
     2641 gccggccgac gagcgcgacg tctaccgcgc ggcgattgac accctggttg agctgcaccg
     2701 cctgccggcg ggcccgttcc tcgactattc gatgagcgag tacatccgcg aggccaagct
     2761 gctgaccgac tggttctgcc cggcgcagaa cctttacatc gacggtccgg gctatgtcgc
     2821 cgcctgggaa aaggtgctgg gcgacatgct gccgcgccag cgccccgggg tggcggtgct
     2881 gcgcgattac catgccgaga acatcatgct gctgggctcg ctggccgagc agggcctgct
     2941 cgatttccag gacgcgctga acggccatcc ggcctacgat ctggtctctc tgctgcagga
     3001 cgcccgccgc gacgtggccc aggagcttga ggccgagatg ttcgactatt acgtcgaaaa
     3061 gaccggcgcc ggcgccgatt tcctggccga ctacgcccgg cttggcgcgc agcgcaacgc
     3121 caagatcgtc ggcatcttcg tccgcctgtg gaagcgcgac ggcaagcccc gctacctcga
     3181 tttcattccc cgggtctggg cctatctgga acgcgatttg tcgcacccgg cgctggcccc
     3241 ggttgcggcc tggttcgatg ccaacatccc ggccgaactg cgcgacgcgg gc
//
LOCUS       DASUDV010000504         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_856602,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000504 DASUDV010000000
VERSION     DASUDV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856602"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1095
                     /locus_tag="VFV30_10910"
     CDS             181..1095
                     /locus_tag="VFV30_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4848644.1"
                     /translation="MTISFKDRVAIVTGAGGGLGRSYALELAKRGAKVVVNDLGGSRD
                     GTGHSDAALKVVEEIKAMGGEAMSNGGSVTDLAQMEEMVAKAKEAWGGVHILINNAGI
                     LRDKSFAKMTMEDWKLVIDVHLNGSANCTKAVWDLMREQAYGRILMTASSTGLFGNFG
                     QANYGAAKLGLAGFTKTLALEGAKYNVRINTLAPVAGTRMTEDLGMPEALFNALKPEN
                     VAPMALYLVSEDAPTNMICGAGAGAYHAAYVTLTPGVALPADERTPEGIAAAWDQIID
                     RTGEIVPQSGGEQSQVVMAALAKIGGLG"
     gene            1167..2207
                     /locus_tag="VFV30_10915"
     CDS             1167..2207
                     /locus_tag="VFV30_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848645.1"
                     /translation="MYSEAELQSAVAAGAISREAAQALRDHATGMRNAPVADEEHFRL
                     ITGFNDIFVTIAAVLLLVACAGIGGAMSAGAGGLLVAGAAWLMAEFFTRKRRMALPSI
                     VLLLAFVGGVIAIPIEIIDSTRPHFSDQVSALIGAGIALLAGGAALVHWRRFMVPITV
                     AAGTAALAATAVALIMAAIQPLVGRDGMENVLLWLLFGAGIVVFGFAMRWDMSDRARE
                     TRRSDVAFWLHLLAAPMIAHPLFHALGVTEGQNISALAALGVVVIYVLLGLVALAVDR
                     RALLVSALAYVLTAMTLLFKNFGMVELNVALTGLVIGSALLSLSAFWGPIRRAVVSPL
                     PDQWRGRLPATA"
     gene            complement(2290..2637)
                     /locus_tag="VFV30_10920"
     CDS             complement(2290..2637)
                     /locus_tag="VFV30_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX4848646.1"
                     /translation="MSAVVTPFPLAAARPAQVGFEREELQRILDLYGRMVAAGLWRDY
                     AMDFGKDAASFCGFRRAAERPEARIEKRPALRAKQGMWTLYGEAGQVLKRGHELSGVL
                     SVLERRLLKVVED"
     gene            complement(2634..3344)
                     /gene="epsC"
                     /locus_tag="VFV30_10925"
     CDS             complement(2634..3344)
                     /gene="epsC"
                     /locus_tag="VFV30_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX4848647.1"
                     /translation="MPGRFITYLDSIRARDPAVRSRWEILLYPGVWALGFHRIAHRLF
                     RARLYLLARLVNHLSRFMTAIDIHPGAKIGRNFFIDHGFVVIGETAEIGDNVTMYQGS
                     TLGGTNPTNGVGGKRHPTIGDGVIISLGAAILGPIHVGKDSRIGANAVVTKDVPEGAT
                     MLGIPAKPTPVEVKPETQAFVPYGTPCSERFDPATQKLEILQCEIETLQKRVAQLLEE
                     RDAQAAKQSSGRKRGDAA"
BASE COUNT          579 a         1109 c         1074 g          620 t
ORIGIN      
        1 agttttgttt cgattcgcag acgcatctag gacttctaga tgacactaat ttgacacctg
       61 cgcgtcgctt gccgctacgg caatgccttc gctcttctac actgttgaaa gccgcggcaa
      121 gccgtgccag tgcagccggc gcttccccag cgggatcaac ctattcttgg agtcaagccg
      181 atgaccattt ctttcaagga ccgtgttgcc attgttaccg gtgccggtgg cggcctgggc
      241 cgttcctacg cgctcgaact cgccaagcgt ggtgccaagg tcgtggtcaa tgacctcggc
      301 ggcagccgcg atggcacggg ccactccgac gccgcgctga aggtcgtcga ggaaatcaag
      361 gcgatgggcg gcgaagccat gtcgaatggc ggctcggtta ccgatcttgc ccagatggaa
      421 gaaatggtcg ccaaggccaa ggaagcctgg ggcggcgtgc acatcctgat caacaacgcc
      481 ggcattctgc gcgacaagag cttcgccaag atgaccatgg aagactggaa gctggtgatc
      541 gacgttcacc tgaacggcag cgccaactgc accaaggcgg tgtgggacct gatgcgtgaa
      601 caggcctatg gccgcatcct gatgaccgcc agcagcaccg gcctgttcgg caacttcggc
      661 caggccaact atggcgcggc caagctgggt ctggcgggct ttaccaagac cctcgccctt
      721 gagggcgcga agtacaacgt tcgcatcaac accctggccc cggtcgccgg cacccgcatg
      781 accgaagacc tcggcatgcc cgaagcgctg ttcaacgcgc tgaagccgga aaacgtcgcg
      841 ccgatggcgc tctacctcgt cagcgaagac gccccgacca acatgatctg cggcgccggt
      901 gccggcgcct atcacgccgc ctatgtcacc ctgacccctg gcgtcgccct gccggccgat
      961 gagcgcacgc cggaaggcat cgccgcggcc tgggatcaga tcatcgaccg caccggcgaa
     1021 atcgttccgc agtcgggcgg cgaacagagc caggtggtga tggctgccct cgccaagatc
     1081 ggcggcctgg gctgatccag tccatactgt attgacacag taacacacat ggcgcatcct
     1141 ccaccgacaa caggtggagg atgcccatgt acagcgaagc cgaactgcaa tccgcagtcg
     1201 cagcaggagc gataagccga gaggcggcgc aagcgctgcg cgatcatgcc acggggatgc
     1261 gcaatgctcc cgtcgccgat gaagaacatt tccggttgat caccgggttc aacgatatct
     1321 tcgtgaccat tgccgcggtg ctgctgctgg tcgcctgtgc cgggatcggc ggggccatgt
     1381 cggcaggagc aggcggcttg ctggtggccg gtgccgcctg gctgatggcc gaattcttca
     1441 cccgcaagcg ccgcatggcc ctgccctcga tcgtcctgct gctggccttt gttggcgggg
     1501 tcatcgcgat cccgatcgag atcatcgatt ccacccgccc gcatttcagt gatcaggtct
     1561 ctgccctgat cggcgcaggc attgccttgc tcgccggtgg ggcggctttg gtccactggc
     1621 ggcggttcat ggtgccgatc acagtcgctg ccggcacggc cgcactcgcc gccacggcgg
     1681 tcgccctgat catggccgca atccagccgc tggttggccg cgacgggatg gaaaatgtcc
     1741 tgctgtggct gctgttcggt gccgggatcg tggtcttcgg ctttgcgatg cgctgggaca
     1801 tgtcggaccg ggcgcgcgaa acgcggcgca gcgatgtggc cttctggctg cacctgctgg
     1861 ctgcgccgat gattgcccac ccgctgttcc atgccctggg cgtgaccgaa ggccagaaca
     1921 tcagcgcgct cgctgcgctg ggtgtggtgg tgatctacgt gctgcttggc ctcgtcgccc
     1981 tggcggtcga tcgccgcgcg ctgctggttt cggcgctggc ctatgtgctg accgccatga
     2041 ccctgctgtt caagaacttc ggcatggtcg agctgaacgt cgcactgacc gggctggtga
     2101 ttggctcggc cctgctcagc ctatcggcct tctggggccc gatccggcgg gcggtggtgt
     2161 cacccctgcc cgatcagtgg cgcggacgac tgccggcaac ggcctgagcg aagcaaggcc
     2221 ctaaacaccg cggggcggcg agctaggctc gccgccccgt ctgcttttgc ttgcttggaa
     2281 ctgccgcgcc tagtcctcga ccaccttcag cagtcgccgt tcgagcacgg agagcacgcc
     2341 agacaattcg tgcccgcgct tcagcacctg cccggcttcg ccataaagcg tccacatgcc
     2401 ctgcttggcg cgcagcgcgg gacgcttttc gatccgcgcc tcaggccgct ccgcagcgcg
     2461 gcgaaagccg cagaagctgg cggcgtcctt gccgaaatcc atcgcatagt cacgccacag
     2521 cccggcggcg accatccggc catagagatc caggatacgc tgcagttcct cccgctcaaa
     2581 cccgacctgg gccggccgcg cggccgcgag cggaaagggc gtgacgactg cgctcaagca
     2641 gcgtcaccgc gcttgcggcc agagctttgc ttggccgcct gtgcatcgcg ttcttccagc
     2701 agttgcgcga cccgcttttg cagggtctca atctcgcact gcaggatttc cagcttctgc
     2761 gtggccggat cgaagcgctc gctgcatggc gtgccatagg gcacgaaggc ctgcgtttcg
     2821 ggctttacct caaccggggt cggcttcgcc ggaatgccca gcatggtcgc gccttcaggc
     2881 acgtccttgg tcaccacggc attggcgccg atgcgcgaat ccttgccgac atggatcggg
     2941 ccaaggatcg ccgcgcccag gctgatgatc acgccatcgc cgatggtcgg gtggcgcttg
     3001 ccgcctacgc cattggtggg attggtcccg cccagggtcg aaccctggta catcgtgacg
     3061 ttatcgccaa tctcggccgt ttccccgatc actacgaacc cgtgatctat gaagaaattg
     3121 cgcccgatct tcgcgcccgg gtggatatcg atcgcggtca tgaagcggct gagatggttg
     3181 accaggcgcg ccagcaaata aagccgcgcg cggaacagcc ggtgggcaat gcggtggaag
     3241 cccagtgccc agacgccggg gtacagcaga atttcccagc gcgagcggac ggccggatcg
     3301 cgggcccgga tcgaatccag ataggtaatg aaacgtcccg gcataagttc ttcccatccg
     3361 ccgcgaatgt cccacagggg gg
//
LOCUS       DASUDV010000505         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_436738,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000505 DASUDV010000000
VERSION     DASUDV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436738"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFV30_10930"
     CDS             <1..555
                     /locus_tag="VFV30_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX4848648.1"
                     /translation="LVLATGGDPAQVFDAVARLQPVRGRLERAAITRSGAPVYVDYAH
                     TPDALEAAIAALRPHCSGRLIVVFGAGGDRDAGKREPMGRIVADQADLGIVTDDNPRG
                     EDPAAIRAMVLRGGAALREVGDRRAAIAAAIAEAGAADIVLVAGKGHEQGQIVGAGDT
                     CRVLPFDDVAVARECAAPSGRDET"
     gene            552..2021
                     /gene="murF"
                     /locus_tag="VFV30_10935"
     CDS             552..2021
                     /gene="murF"
                     /locus_tag="VFV30_10935"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX4848649.1"
                     /translation="MNALARILAWPADETDQLGLALWDAVEIARATGGVAVGEFQVSG
                     VEIDSRDVVPGDLFFALKGESMDGHRFVEMAFAKGAAAAVVDRPVNGPHILVDDTAAA
                     LGRLAAAARARAEAQIIGVTGSVGKTGVKEAIFAALDRSSRGNAHRSVKSYNNHVGVP
                     LSLARMAPRSKFGVFEMGMNHAGEIAALTAQVRPHVAVITTIAPAHIENLGSIEAIAD
                     AKAEIFEGLEPGGTAVIPADVPQFAQLKAAAQRLGHAVVAFGRSAEADVRLLDAVPAA
                     GGGSLVTADLGDRRVCYTVAEPGDHWIVNSLAVMAAVRAAGGDLGAAGLALAEMQGLK
                     GRGQRHWLTAAGGDGSGRALLIDESYNANPASMKATLAQLGVTPARRRIAVLGAMKEL
                     GEHGPAYHAALIEPLTASGAVHAVLVGPEMAALADELGKAAAAALGSTPGFAHCETAA
                     EALEALRSYGVEGGDAILVKGSNSVGLGTVVAALAQNKD"
     gene            2031..>2728
                     /gene="mraY"
                     /locus_tag="VFV30_10940"
     CDS             2031..>2728
                     /gene="mraY"
                     /locus_tag="VFV30_10940"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX4848650.1"
                     /translation="MLYLIAQYFQFEGLANLIRYQTFRAGAALMTALVIGLIIGPRFI
                     AMLRVRQGKGQPIREDGPQSHLAKRGTPTMGGLMILVALVVSMLLWMDLTSPLVWACL
                     AVTIGFGAIGFLDDYDKVKKRTTAGVPARVRLGAEFFVAGIASWIMVSQIDTNLYIPF
                     LSDRYIPLGWFYYVFAAFVIVGAGNAVNLTDGLDGLATMPVIIAAGTFAIIAYLAGRA
                     DFSAYLGIPHVPRAG"
BASE COUNT          394 a          908 c          996 g          430 t
ORIGIN      
        1 ctggtcctgg cgaccggcgg cgatccggcc caggtgttcg acgccgtggc ccggctgcag
       61 ccggttcgcg gccggctgga acgcgccgcg atcacccgct cgggcgcgcc ggtctatgtc
      121 gattacgccc acacgcccga cgcgctggaa gccgccatcg cggcgctgcg cccgcattgc
      181 agcggccgcc tgatcgtggt cttcggcgcc gggggcgatc gcgacgccgg caagcgcgag
      241 ccgatgggcc ggatcgtggc cgatcaggcc gaccttggca tcgtgaccga tgacaacccg
      301 cgcggcgagg atcccgccgc gatccgcgcc atggtgctgc ggggcggggc ggccttacgc
      361 gaagtgggcg accgccgcgc cgcgattgcc gccgcgattg ccgaggccgg ggcggccgat
      421 atcgtgctgg tggccgggaa gggccacgaa cagggacaga tcgtgggcgc cggcgacacg
      481 tgccgcgtcc tgccgttcga tgacgtcgcc gtggccaggg agtgcgcagc tccttccggg
      541 agggatgaga catgaatgcg cttgccagaa tccttgcgtg gcctgccgac gaaaccgacc
      601 agctgggcct ggcgctgtgg gacgcggtgg agatcgcccg cgcgaccggc ggggtggccg
      661 ttggcgaatt ccaggtttcc ggcgtggaaa tcgacagccg cgacgtggtt cccggggacc
      721 tgttctttgc cctgaagggc gagagcatgg acgggcaccg ctttgtcgag atggcctttg
      781 ccaagggcgc ggccgcggcg gtggtcgacc ggccggtgaa cggcccgcac atcctggtgg
      841 acgataccgc cgccgcgctg gggcgtctgg ccgcggcggc ccgtgcgcgc gccgaagcac
      901 agatcatcgg ggtgaccgga tcggtcggca agaccggcgt caaggaggcg atcttcgccg
      961 cgctggaccg ctccagccga ggcaacgccc accgctcggt caagagctac aacaaccatg
     1021 tcggggtccc gctcagcctg gcccgcatgg ccccgcgcag caagttcggg gtgttcgaaa
     1081 tgggcatgaa ccacgccggc gagatcgccg cgctgaccgc ccaggtccgc ccccacgtgg
     1141 cggtgatcac caccattgcc cccgcccata tcgagaacct gggcagcatc gaggcgattg
     1201 ccgatgccaa ggcggagatc tttgaagggc tggagccggg cggaacggcg gtcattcccg
     1261 ccgatgtccc gcagttcgcc cagctgaagg ccgcggcgca gcggctgggc cacgcggtcg
     1321 tcgcctttgg ccgttcggcc gaggccgatg tccgcctgct cgatgccgtc cccgctgctg
     1381 gcggcggatc gctggtgacg gccgatctgg gcgaccggcg ggtctgctac accgtggccg
     1441 aaccgggcga ccactggatc gtcaattcgc tggccgtcat ggccgcggtg cgcgccgccg
     1501 ggggcgatct gggtgcggct ggccttgcgc tggcggaaat gcagggtctc aagggccggg
     1561 gccagcgcca ctggctgacg gccgcgggcg gcgacggatc gggccgggcg ttgctgatcg
     1621 acgaaagtta caacgccaac ccggcctcga tgaaggccac cctggcccag ctgggggtca
     1681 ctccggcccg gcgccggatc gcggtgctgg gcgcgatgaa ggaactgggc gaacacggac
     1741 cggcctatca cgccgcgttg atcgagccgc tgaccgcctc gggcgcggtc catgccgttc
     1801 tggtcggccc ggagatggcg gcactggccg acgaactggg gaaagcggcg gctgccgcgc
     1861 ttggcagcac gcccggcttc gcccattgcg aaacggcggc cgaagcgttg gaggcgctcc
     1921 ggtcgtatgg cgtggaaggc ggcgacgcga tcctcgtcaa ggggtcgaac tcggtcgggt
     1981 tggggacagt cgtcgccgcg ctcgcgcaaa acaaggacta aggcagggca atgctctacc
     2041 tgatcgcgca atatttccag ttcgaagggc tggcgaacct tatccggtac cagaccttcc
     2101 gcgccggggc ggcgctgatg acggcgctgg tgatcggcct gatcatcggc ccccggttca
     2161 ttgccatgct gcgggtccgc cagggcaagg gccagccgat ccgcgaggac gggccgcaaa
     2221 gccacctcgc caagcgcggc acgccgacca tgggcgggct gatgatcctg gtggcgctgg
     2281 tcgtctcgat gctgctgtgg atggacctga ccagcccgct ggtctgggct tgccttgccg
     2341 tcacgatcgg ttttggcgcg atcgggttcc tggacgatta cgacaaggtc aagaagcgga
     2401 ccaccgccgg ggtcccggcg cgggtgcggc tgggcgcgga attcttcgtc gccgggatcg
     2461 cctcgtggat catggtcagc cagatcgaca ccaacctcta catcccgttc ctgtcggatc
     2521 gctacatccc gctgggctgg ttctattacg tctttgccgc cttcgtgatc gtcggcgcgg
     2581 gcaacgcggt gaacctgacc gacgggcttg atggcctggc caccatgccg gtgatcatcg
     2641 cggcggggac cttcgcgatc attgcctatc tggccggccg ggcagacttt tccgcctatc
     2701 tcggcattcc gcatgtgccg cgcgccgg
//
LOCUS       DASUDV010000506         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1077802,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000506 DASUDV010000000
VERSION     DASUDV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077802"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /gene="pepN"
                     /locus_tag="VFV30_10945"
     CDS             complement(<1..2336)
                     /gene="pepN"
                     /locus_tag="VFV30_10945"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX4848651.1"
                     /translation="MDISSAPPAPTIIRREDYQPPAWLVPEVTLAFTLGIEETRVVAT
                     LKVTRNGTDQTLRLHGDGIAAESVLVDGQPSNAWRMDGADLLVDLSGAAHEVAIATAI
                     NPAANTQLMGLYASNGMLCTQCESEGFRRITFFPDRPDVLSVYTVHMEGPKDLFPVLL
                     SNGNCTAAGDGPLLGTHHATWHDPWPKPSYLFALVAGDLVANHDTFTTCSGRKVDLNI
                     WVREGDQDRTHHAMQALKDSMKWDEEVFGREYDLDLFNIVAVSDFNMGAMENKGLNVF
                     NTRYILADPDTATDMDYDGIEGVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVLRDQL
                     FSQDMGSEAVKRIEDVRVLRGAQFPEDSGPLAHPIRPDSYQEISNFYTATVYNKGAEV
                     IRMMRTLVGPQRFRAGTDLYFDRHDGEAATCEDFVKAIEDGAGIDLAQFRLWYSQAGT
                     PKVTARLVHEGDVATLTLSQTVPPTPGQPDKQPMVIPLRLALFDPATGQHQGERLVVL
                     DRAEMEFAFPGFPARPVLSLNRGYSAPVSLETDTTRADLQFLAAHDDDPFARYEALQQ
                     LAVQHLLAVIGGDADRDGAAALVAALRAVVADPALDDLMRGELMMLPGEAYLAEQFQR
                     ADPGAIRAAREALKAELGQALTDDLVGLHARVSAVPYGLDAAARGARKVKTQALVLLA
                     AGVPDLGAELAATQFDAADNMTDRQGALMVLCGLDTPLREERLAEFHARYAGNALVID
                     KWFSLQAGSLHPDVLAHIRALADHPDFTLTNPNRVRAL"
BASE COUNT          381 a          793 c          847 g          406 t
ORIGIN      
        1 tagagcgcgc ggacccggtt ggggttggtc agggtgaaat ccggatgatc ggccagggcg
       61 cggatatggg ccagcacatc ggggtgaagc gatccggcct ggagcgagaa ccacttgtcg
      121 atcaccagcg cgttgccggc atagcgggcg tggaactcgg ccagccgctc ttcgcgcagc
      181 ggggtgtcga gcccgcacag caccatcagc gcgccctgcc ggtcggtcat gttgtcggcc
      241 gcgtcgaact gggtggcggc cagctcggcc cccaggtccg gcaccccggc cgccagaagg
      301 accagcgcct gggtcttaac cttgcgggcc ccgcgcgcgg ctgcatcgag accataggga
      361 accgcgctga cccgggcatg cagcccgacc agatcgtcgg tcagcgcctg gcccagttcg
      421 gctttcagcg cttcgcgcgc cgcgcggatc gcgccgggat cggcccgctg gaactgttcg
      481 gccagatagg cctcgccggg cagcatcatc agctcgccgc gcatcagatc gtcaagcgcc
      541 ggatcggcaa ccaccgcccg cagcgcggcg accagggccg cggcgccgtc gcggtccgca
      601 tcgccgccga tcacggccag caggtgctgg accgccagtt gctgcagcgc ctcgtaccgg
      661 gcgaagggat cgtcgtcgtg cgcggccagg aactgcaggt cggcgcgggt ggtgtcggtc
      721 tccagcgaaa ccggggcaga atagccgcga ttgagcgaga gcaccggacg ggccggaaag
      781 ccggggaagg cgaattccat ttcggcccgg tccagcacga ccagccgctc gccctggtgc
      841 tggccagtgg ccggatcgaa cagcgcgagg cgcagcggga tcaccatcgg ctgcttgtcc
      901 ggctggccgg gcgtgggcgg aaccgtctgg ctgagcgtca gggtggcgac atcgccctca
      961 tggaccagcc gcgcggtgac tttcggggtc ccggcctggc tgtaccacag gcggaactgg
     1021 gccaggtcga tcccggcgcc gtcctcgatc gccttcacga aatcctcgca ggtcgcggcc
     1081 tcgccgtcgt ggcgatcgaa atagaggtcg gtgccggcgc ggaaccgctg cggccccacc
     1141 agggtgcgca tcatgcggat cacctcggcc cccttgttat agaccgtggc ggtgtagaag
     1201 ttgctgattt cctgatagga atcgggccgg atcgggtgcg ccagcgggcc cgaatcctcg
     1261 gggaactggg ccccgcgcag cacccgcacg tcctcgatcc gcttgaccgc ttcggacccc
     1321 atgtcctggc tgaacagctg gtcgcgcagc acggtgaagc cttccttcag gctcagctgg
     1381 aaccagtcgc ggcaggtcac gcggttgccc gaccagttgt ggaagtattc gtgggcaacc
     1441 acgccttcga tcccgtcata gtccatgtcg gtcgcggtat cgggatcggc caggatgtag
     1501 cgggtgttga agacgttgag gcccttgttc tccatcgccc ccatgttgaa gtccgacacg
     1561 gcgacgatgt tgaacaggtc gagatcgtat tcgcggccga acacctcctc gtcccacttc
     1621 atcgaatcct tcagcgcctg catggcgtga tgggtccggt cctgatcgcc ctcgcggacc
     1681 cagatgttga gatcgacctt gcggcccgag caggtggtga aggtgtcatg gttggcgacc
     1741 agatcgcccg cgaccagtgc gaagaggtag ctcggcttgg gccagggatc gtgccaggtg
     1801 gcgtggtggg tgccgagcag cggaccatcg cccgcagccg tgcagttgcc gttggacagc
     1861 aggaccggga acaggtcctt cggcccttcc atgtggacgg tatagaccga cagcacgtcg
     1921 ggccggtcgg ggaagaaggt gatccgccgg aacccttcgg attcgcactg ggtgcagagc
     1981 atcccgttcg aggcatagag gcccatcagc tgggtgttgg cggccgggtt gatcgcggtc
     2041 gcgatcgcaa cctcgtgcgc cgcgccggac aggtcgacca gcaggtcggc cccgtccatc
     2101 cgccaggcgt tgctgggctg gccatcaacc agcacgctct ccgccgcaat cccgtcaccg
     2161 tgcagccgca gggtctggtc ggtcccgttg cgggtgacct tcagcgtcgc gaccacccgg
     2221 gtctcctcga tcccgagggt gaaggccagg gtgacttcgg ggaccagcca ggccggcggc
     2281 tgatagtctt cgcggcggat gatcgtgggg gcggggggcg ctgatgaaat gtccatggcc
     2341 cctttctagc catgccgctt ccgattgcca gcgccgccgc ctatgaagcg gggcaatgcg
     2401 acgcattttc atctttggcc tgggcta
//
LOCUS       DASUDV010000507         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_790470,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000507 DASUDV010000000
VERSION     DASUDV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790470"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VFV30_10950"
     CDS             <1..1012
                     /locus_tag="VFV30_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX4848652.1"
                     /translation="DGEGQPIDGGAPIHTRSAWPAGGVRTGALDRSPVRLPFDTGVRA
                     LNALTTFGRGQRIGIMAGSGVGKSVLLDMVARGAEAEVVVVGLIGERAREVSDFVERH
                     MGEEKRARTAVVAVPADHAPNLRLRGAMLATSLAEHFRSQGRQVLLIMDSLTRVAHAA
                     REIALLLGEPGAARGYPPSALATITKLVERAGNSAASGGSITGIYTVLADGDNQDDPV
                     VDTARSILDGHVVLSRELAQRGQYPAIDVAASLSRVMADIASSEHATLARAFRALIAS
                     YEANRDLVLMGAYRAGADPVLDRAIDMHAALGAFLSQDTGTTVSLAQSIAELTGLLRD
                     AR"
     gene            1002..1427
                     /locus_tag="VFV30_10955"
     CDS             1002..1427
                     /locus_tag="VFV30_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848653.1"
                     /translation="MRADKAQIRRLQRIERLRALARQDAARAAAQAEGTLAQLVRLSA
                     RTGAMRDEYGARTSIGDGLALLQHHAFVSGLSAIHHLTSADATQARSTADRLQQELSL
                     AERRRAAVEQRIDQASQALAQRGAGEPTGSRRATGTLFE"
     gene            1462..>2497
                     /locus_tag="VFV30_10960"
     CDS             1462..>2497
                     /locus_tag="VFV30_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848654.1"
                     /translation="MIPPLPAQTSSTLLPATEMVFNPHPGDPEGLDFGALLDLGAQSA
                     PTQPAPALPGAESLAAPADLPGALPVGGKILPVELPPTASPQAGADPAPSMAIEHQPV
                     FLPRIVARTAADPESPVREEPAAKAESGAEQTTRNDPPIQQPLPPTPQPVIVALAAPV
                     PAVPTSGQLRDRDGPDIASRPTVSSAHTTSPFPARTAEPRVQMEQPRPPALSVTASKP
                     DPVVPSPASPQALAPPASADKLAPVAQRQETQFSLALPAARQSTARTDPAQVPVAGTE
                     AAQPQLALPQYQPPATPAASGARPSLQTDAAPRPHDFAGLIDRLVAARDAVQPQAVSI
                     AVQHADFGEIS"
BASE COUNT          425 a          909 c          803 g          360 t
ORIGIN      
        1 cgatggtgaa ggccagccga tcgatggcgg tgcgccgatc catacgcgca gtgcctggcc
       61 ggctggcggc gtgcgcaccg gcgcgctcga tcgcagtccg gtgcggctac ccttcgatac
      121 cggcgtcagg gcgctcaacg cactgaccac attcgggcgc ggccagcgga tcgggatcat
      181 ggccggatcg ggcgtgggca aatcggtgct gctggacatg gtcgcccgcg gcgccgaggc
      241 cgaagtggtg gtggtcgggc tgatcggcga acgcgcccgc gaggtgtcgg actttgtcga
      301 acggcacatg ggcgaagaga agcgcgcccg caccgccgtg gtggcggttc cggccgatca
      361 tgcccccaac ctcagactgc gcggggcaat gctggcgacc agccttgccg agcacttccg
      421 cagccagggc cgccaggtgc tgctgatcat ggacagcctg acccgggtcg cccatgccgc
      481 gcgcgaaatc gcgctgctgc tgggcgaacc cggcgcagcg cgaggctacc ccccttcggc
      541 gctggcgacg atcaccaagc tggtcgagcg cgccggcaat tcggcagcca gcggcggctc
      601 gatcaccgga atctacaccg tgcttgccga tggcgacaat caggacgacc cggtggtcga
      661 taccgcacgc tcgatccttg atgggcacgt cgtcctgagc cgcgaactgg ctcagcgcgg
      721 gcagtatccg gcaatcgatg ttgcagcctc gctcagccgg gtcatggccg atatcgccag
      781 ttccgaacac gcgacgctgg cccgcgcgtt tcgcgcgctg atcgccagtt acgaagcgaa
      841 ccgcgatctg gtgctgatgg gggcctaccg cgccggagcc gatccggtgc ttgaccgtgc
      901 gatcgatatg cacgccgcgc ttggcgcctt cctcagccag gacaccggca caaccgtttc
      961 gctggcgcaa agcatcgccg aactgaccgg actgcttcgc gatgcgcgct gacaaggcgc
     1021 agatacgccg actgcaacgg atcgaacgcc tgcgcgcgct tgccaggcag gatgcggcgc
     1081 gggccgcggc gcaggccgaa gggaccctgg cccaactggt caggctttcg gcccgaaccg
     1141 gggcgatgcg cgacgaatac ggtgcccgca ccagtattgg cgacggcttg gcattgctcc
     1201 agcaccacgc cttcgtcagc ggactatcgg cgatccatca ccttaccagc gccgatgcca
     1261 cccaggcccg ctccaccgcc gaccggttgc aacaggaact gtcgctggcc gaacgccgcc
     1321 gcgccgcagt tgaacagcgc atcgaccagg ccagccaggc gctggcgcag cgcggcgcgg
     1381 gcgagccaac cgggagccgc cgggcaactg gcacgctctt tgaatagctc ttggcgaacc
     1441 cagtcgaagg accaagcgaa catgattccg ccgcttcctg cccaaacctc gagcacgctt
     1501 ctgccagcga cggaaatggt gttcaatccg catccggggg atccagaagg gctggatttc
     1561 ggcgctttgc tcgatcttgg cgcacagtcc gcaccgacac agccggcccc cgcgctaccc
     1621 ggagccgaaa gcctcgcagc accagcagac ctgcccggcg ccttgccggt tggcggcaag
     1681 atcttgccgg tcgagttgcc gccgaccgct tcgccgcaag caggtgcgga cccggcgccg
     1741 agcatggcga ttgagcacca gccagtgttc ctaccccgga tcgtggcgcg cacagccgca
     1801 gacccggaaa gcccggtacg cgaggagcct gctgccaaag cagaatccgg agcggagcag
     1861 accaccagga atgatccgcc aatccagcaa cccctaccgc ccacacccca accggtaatc
     1921 gtcgcgcttg cagcgccggt tccggccgtg ccgacaagcg gccagctgcg cgaccgcgac
     1981 gggccagaca ttgcctcgcg tccgaccgtg tcctcggccc acacgacatc gcccttcccg
     2041 gccagaaccg ccgagccccg tgtgcagatg gagcagcctc gtcctccagc cctctccgtc
     2101 accgcttcca agcctgatcc ggtggtgccg tctccagcat cgcctcaggc gcttgcgccg
     2161 cccgccagcg ccgacaagct ggcccccgtt gcccagcgcc aggaaaccca gttcagcctt
     2221 gcccttcccg cagcccggca gagcactgcc cggaccgatc cggcgcaggt tccggtagcc
     2281 ggaacagagg ccgcccagcc gcagttggca ctgccgcaat atcagccccc ggcaaccccg
     2341 gcggcgtctg gtgcgcgacc cagccttcaa accgacgccg caccgcgtcc gcatgatttt
     2401 gccggcctga tcgaccggct ggtggctgcg cgcgatgcgg tgcagccgca ggcggtctcg
     2461 atcgcggttc agcatgccga tttcggcgaa atcagcc
//
LOCUS       DASUDV010000508         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1784593,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000508 DASUDV010000000
VERSION     DASUDV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784593"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..631
                     /locus_tag="VFV30_10965"
     CDS             119..631
                     /locus_tag="VFV30_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX4848655.1"
                     /translation="MKLARLIAAVLALLAAPLTAPATAEAPGSFNQRILSAHNGERVR
                     LGLAPLQWNPMLAAQAGVWARDLAARGAFEHAKDRLGAGENLWGGTAGAFAPEAMIGA
                     FLAEKQYFRPGTFPDVSTTRRWQDVGHYTQIVWRGTREVGCAVATGKGRDVLVCRYFP
                     AGNVVGQPVF"
     gene            complement(628..1257)
                     /gene="rdgB"
                     /locus_tag="VFV30_10970"
     CDS             complement(628..1257)
                     /gene="rdgB"
                     /locus_tag="VFV30_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX4848656.1"
                     /translation="MTRRLGSETLVIATHNAGKLKEISALLAPYGLNCISAGSLGLPE
                     PAETGKTFVENALIKARAASESSGLPALADDSGLCVDALGGRPGVYTADWAERQWFEG
                     PAGRDWYMAMGKVEGLLCEKGPDVDRSAHFACVLAIAWPDGESAVYEGKVPGSLTWPP
                     RGTLGFGYDPVFVPRGQDQTFAEIDPAQKHASSHRAAAFSLLVAEQFGG"
     gene            complement(1254..1970)
                     /gene="rph"
                     /locus_tag="VFV30_10975"
     CDS             complement(1254..1970)
                     /gene="rph"
                     /locus_tag="VFV30_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX4848657.1"
                     /translation="MRPSGRAPDEMRAISFEPHYTKHAEGSVLVSFGHTKVICTASVE
                     ERLPPWLRGKGEGWVTAEYSMLPRATHTRGSREAAKGKQSGRTQEIQRLIGRSLRAVT
                     DLKKLGERQITLDCDVIQADGGTRTAAISGAWVALRIAVAKLMAAGAITEDPLTRKVA
                     AVSCGIFNGTPVLDLDYDEDSAADADANFVLIEGGQIAEVQATAEGATYDEEALLRLL
                     RLARIGCERIFTAQGEAAKG"
     gene            2041..>2282
                     /locus_tag="VFV30_10980"
     CDS             2041..>2282
                     /locus_tag="VFV30_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848658.1"
                     /translation="MRIWKWLAAIALAASPAVAVPAVQAPDKLAERMSEAPEVALSRA
                     MNGLQLKTKAHDETWRQGKANWSVDLDRGVIEFDND"
BASE COUNT          367 a          765 c          771 g          379 t
ORIGIN      
        1 catggctgtt gaagtcgcaa tagggacact ttgcgaggca gaacggccaa tggatgtaga
       61 gcgcgcgggc catgctggtc ttcaggctag cttaacgccg caatgtcaag gaagacccat
      121 gaagcttgcc cgcctgattg ccgccgttct ggccttgctg gccgcgcctt tgaccgcgcc
      181 tgcaaccgcg gaagccccgg gatcgttcaa ccagcggatc ctctccgccc acaacggcga
      241 acgggtacgc ctgggccttg cgccgctgca atggaacccg atgctggccg cccaggccgg
      301 ggtctgggcg cgcgatctcg ccgcgcgcgg agcctttgag cacgcgaaag accggctcgg
      361 cgcgggcgag aacctgtggg gcggaaccgc cggagccttc gcgcccgaag cgatgatcgg
      421 ggccttcctg gccgagaagc agtatttccg ccccggcacc tttccggacg tgtcgaccac
      481 ccggcgctgg caggatgtgg gccactacac ccagatcgtc tggcgcggca cccgcgaggt
      541 tggctgcgcg gtggcgacgg ggaaggggcg tgacgtgctg gtctgccgct attttccggc
      601 cgggaacgtg gtcggccagc cggtgttcta acccccgaac tgctctgcca ccagcagtga
      661 aaaggccgcg gcacggtggc tgctggcgtg tttctgcgcc ggatcgatct cggcaaaggt
      721 ctggtcctgg ccccgcggca cgaaaaccgg gtcatagcca aaacccagcg tgccgcgcgg
      781 cggccaggtg agcgagcccg gcaccttgcc ttcgtagacc gcgctttcgc catcgggcca
      841 ggcaatcgcc aggacgcagg cgaagtgggc gctgcggtcc acgtccggcc ccttttcgca
      901 cagcagccct tcgaccttgc ccatcgccat gtaccaatcg cggcccgccg ggccttcgaa
      961 ccattgccgc tcggcccagt cggcggtgta gaccccgggc cgcccgccca gcgcatcgac
     1021 gcagaggcca gaatcgtcgg ccagcgcggg caggcccgag ctttccgagg cggcgcgggc
     1081 cttgatcagg gcgttctcga caaaggtctt cccggtctcg gccggctccg gcaggcccag
     1141 cgatccggcc gaaatgcagt tcagcccata aggcgcgagc agagccgaaa tctctttcag
     1201 cttgcccgcg ttgtgggtcg cgatcaccag cgtttcggag ccgagccggc gggtcatccc
     1261 tttgccgctt cgccttgcgc ggtgaagatc cgctcgcacc cgatccgggc gaggcgcagc
     1321 agccgcagca gggcttcctc gtcataggtc gcgccttcgg cggtggcctg aacctcggcg
     1381 atctggccgc cttcgatcag gacgaagttg gcgtcggcat cggccgcgct gtcttcgtca
     1441 tagtcgagat cgagcaccgg ggtgccgttg aaaatcccgc agctgacggc cgcaaccttg
     1501 cgggtcagcg ggtcttcggt gatcgcaccg gccgccatca gcttggccac cgcgatccgc
     1561 agcgccaccc aggcgcccga gatcgcggcg gtgcgggtgc cgccatcggc ctggatcacg
     1621 tcgcaatcga gcgtgatctg ccgctcgccc agcttcttca gatcggtcac ggcgcgcaag
     1681 gagcggccga tcaggcgctg gatttcctgg gtgcgcccgc tctgcttgcc cttggccgct
     1741 tcgcggctgc cgcgggtgtg ggtggcgcgg ggcagcatcg agtattccgc cgtaacccag
     1801 ccttcgccct tgccgcgcag ccacggcggc agccgctctt ccaccgaggc ggtgcagatc
     1861 accttggtgt ggccaaagct gaccagcacc gatccttcgg catgcttggt atagtgcggt
     1921 tcaaagctga tagcgcgcat ctcgtcgggc gcgcggccgg aaggtcgcat ggggtactcc
     1981 tgttgctttg ccaaccgcat aggctaatcc tgagtccgat caaggcggaa aggggccggt
     2041 atgcgcatct ggaaatggct tgcggctatc gccctggcgg cgagcccggc agtggccgtg
     2101 ccggcggtgc aagcgccgga caaactcgct gagcgcatga gcgaagcgcc ggaagtggca
     2161 ctcagccgcg cgatgaacgg tctgcagttg aagaccaagg cgcatgacga aacctggcgg
     2221 caagggaaag ccaactggag tgtcgacctc gaccgcggcg tgatcgagtt cgacaacgac
     2281 ag
//
LOCUS       DASUDV010000509         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_614053,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000509 DASUDV010000000
VERSION     DASUDV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614053"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..374
                     /locus_tag="VFV30_10985"
     CDS             135..374
                     /locus_tag="VFV30_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX4848659.1"
                     /translation="MTLDQLPLGTRARIVAVDWLRLVPEEALRLQALGIDAGARIAVD
                     HRGIFGTRDPIALAIGRMTVALRRSHARAIEVELL"
     gene            371..2209
                     /locus_tag="VFV30_10990"
     CDS             371..2209
                     /locus_tag="VFV30_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX4848660.1"
                     /translation="MTLRTAALVGNPNAGKSALFNALTGARQKIANYPGVTVERKAGR
                     LTLPSGEAIELTDLPGSYGLNATSPDEEVTRRVILGELPGEPAPQVLVVVLDASNLEQ
                     HLVFAQEVIALGRPTVVALNMVDLAERDGLVIDPAALEEALGVPVIPTVAVRRRGLSE
                     LAAAIAVAEERLPEPRHPHTTLTERRVSAQAIAEAAILSETAQHRLHARLDQFLLHPW
                     FGPLVLFALLFVVFQAVFTWATPFADALEGGIAAVADQVRASVPQGLVRDFITEGVLS
                     GVGSVVVFLPQIVILFFFILAMEASGYMARAAFLMDRLMARVGLSGRSFIPLLSSFAC
                     AIPGIMATRSIADPKDRLTTILIAPLMTCSARLPVYAVIIAAFIPNRAVLPGVGLQGL
                     VLFGLYVVGIVGAMIVALVLRRSVTKGAASGFIMELPKYQLPRAKDLALGLWQRAWIF
                     LRRAGTIIFTVTVVLWLLLNFPRAGEGQNQVDASIAGKLANGLAVVVEPIGFNRDIAL
                     SLIPAMAAREVAVASLATSYAVAAEGEEQAALALGEQLRTKWSLPTALAFLAWFVFAP
                     QCMSTIAVARRETNGWKWPLFMLLYLFSLAYIFAGITYWSAVALGL"
     gene            2224..2751
                     /gene="ssb"
                     /locus_tag="VFV30_10995"
     CDS             2224..2751
                     /gene="ssb"
                     /locus_tag="VFV30_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX4848661.1"
                     /translation="MAGSVNKVILIGNLGQDPEVKSFQNGGRIANLRIATSENWKDRA
                     TGERKERTEWHTVVLQSDGLVGVAEKYLRKGSKVYIEGQLRTRKWQDASGNDRYTTEI
                     SVGMNGVLTMLDGAPGGGQGGGMGGGQRSGGGGGDWGGGSGGGQRSGGSDFGGGSGGG
                     SRGGFADDLDDDIPV"
     gene            complement(2753..>3261)
                     /locus_tag="VFV30_11000"
     CDS             complement(2753..>3261)
                     /locus_tag="VFV30_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX4848662.1"
                     /translation="SEFSRTYDPRLLPGSAVELAATEAECAALAKRFGLVRIDRLSAT
                     VDLEREANGASAKGRLTAAIVQSCAVSGEDLPVSIDEPVSLHFVPAGAPSSPDEEIEL
                     EEAELDEIEFTGSLFDLGEALAQTLALAIDPYAIGPNAEEARRKAGLLDKDTSGPFAA
                     LAALKKPD"
BASE COUNT          464 a         1084 c         1114 g          599 t
ORIGIN      
        1 acttgggatc actcgcctgt gccccaccac cacctgcggt ggtccccctc ccccaagggg
       61 gaggaatttg acctcgatca attattgcga atgacttgca acaagcgccg cgctgtggca
      121 gcgggcgttc tcgcatgaca ctcgatcagc tcccgcttgg cacccgtgcc cgcatcgtcg
      181 cggtcgattg gctgcgtctg gtccctgagg aggcgctgcg cctgcaggcc ctggggatcg
      241 acgcaggcgc gcgaatcgcg gtcgaccatc gcggcatttt cggcacgcgc gatccgattg
      301 ctctggccat cgggcggatg accgtggcgc tgcgccgctc ccatgcccgc gcgatcgagg
      361 tggaactgct gtgaccctgc gcaccgcggc cctggtgggc aaccccaacg ccggcaagag
      421 cgcgctgttc aacgcgctga ccggcgcgcg ccagaagatc gccaactatc ccggcgtcac
      481 cgtggagcgg aaggccgggc ggctgaccct gccctcgggc gaagcgatcg agctgaccga
      541 cctgccgggc tcctacggcc tgaacgccac cagcccggac gaagaggtca cccgccgggt
      601 gatcctgggc gaactgccgg gtgagcctgc gccgcaggtt ctggtcgtgg tgctcgatgc
      661 ctcgaacctc gaacagcacc tggtctttgc ccaggaagtg atcgcgctgg ggcggccgac
      721 cgtggtcgcg ctcaacatgg tcgacctggc cgagcgcgac gggctggtga tcgatcccgc
      781 cgcgctggaa gaggcgctgg gcgtgccggt cattccgacc gtcgccgtgc gccgccgcgg
      841 ccttagcgaa ctggccgccg ccatcgccgt ggccgaagaa cgcctgcccg aaccgcgcca
      901 cccgcacacc acgctgaccg agcgccgggt ttcggcccag gcgattgccg aggccgcgat
      961 cctgtccgaa accgcgcagc atcgcctgca tgcacggctg gatcagttcc tgctgcaccc
     1021 gtggtttggc ccgctggtgc tgtttgccct gctgttcgtg gtgttccagg cggtctttac
     1081 ctgggcgacg ccctttgccg atgcgctgga aggcgggatc gctgccgttg ccgaccaggt
     1141 ccgcgcttcg gtgccgcaag ggctggttcg tgatttcatt acggaggggg ttctttccgg
     1201 cgtcggctcg gtcgtggtct tcctgccgca gatcgtcatc ctgttcttct tcatcctggc
     1261 gatggaggct tcgggctata tggctcgcgc ggccttcctg atggaccggc tgatggcccg
     1321 cgtcggcctt tcgggccgca gcttcatccc gctgctctcc agctttgcct gcgccattcc
     1381 cgggatcatg gcgacgcgca gcatcgccga tcccaaggac cgcctgacca cgatcctgat
     1441 cgccccgctg atgacctgtt cggcgcgcct gccggtctat gccgtaatca tcgccgcctt
     1501 cattcccaac cgcgcggtcc tgcccggtgt gggcctgcag ggcctggtgc tgttcgggct
     1561 ctatgtggtg ggcattgtcg gcgcgatgat cgtcgcgctg gtgctgcgcc gttcggtcac
     1621 caagggcgcg gcctcgggct tcatcatgga gctgcccaag taccagctcc cgcgcgccaa
     1681 ggacctggca ttgggcctgt ggcagcgtgc ctggatcttc ctgcgccgcg ccggcacgat
     1741 catcttcacg gtcacggtgg tgctgtggtt gctgctcaac ttcccgcgcg ccggggaagg
     1801 gcagaatcag gtcgatgcct cgatcgccgg caagctggcc aatggcctcg ccgtggtggt
     1861 cgagccgatc ggcttcaacc gcgacatcgc gctctcgctg atcccggcga tggccgcgcg
     1921 cgaagtcgct gttgcctcgc tcgccacatc ctatgccgtg gcggccgagg gggaggaaca
     1981 ggccgcgctg gcgctgggtg agcagctgcg gacgaagtgg agcctgccga ccgcgctggc
     2041 cttcctcgcg tggttcgtgt tcgccccgca gtgcatgagc acgatcgctg tggcacggcg
     2101 cgagacgaat gggtggaaat ggcccctgtt catgttgctc tacctgttct ccctcgccta
     2161 catcttcgcg ggaatcacct attggagcgc cgtcgcgctc gggttgtagc ggagcgtttt
     2221 tcgatggcag gtagcgtcaa caaggtaatc ctgatcggca atctgggcca ggatcctgag
     2281 gtcaagagct tccagaatgg gggccggatc gccaacctgc gaatcgccac cagcgaaaac
     2341 tggaaggacc gcgccaccgg tgagcgcaag gaacggaccg agtggcacac ggttgtgctc
     2401 cagtccgatg gcctggtcgg cgttgcggaa aagtacctgc gcaagggcag caaggtttac
     2461 atcgaaggcc agctccgcac acgcaagtgg caggatgctt cgggcaacga tcgctacacc
     2521 accgagattt cggtcggcat gaacggcgtc ctcaccatgc tggatggcgc gccgggtggc
     2581 ggccagggtg gcggcatggg cggcggccag cgctctggcg gcggtggtgg tgactggggc
     2641 ggcggctccg gcggcggcca gcgttcgggc ggaagcgact tcggcggcgg ttctggcggc
     2701 ggctcgcgcg gcggcttcgc cgacgatctg gacgacgata tcccggtctg agctagtccg
     2761 gcttcttcag cgccgccagc gcggcaaacg gcccgctggt gtccttgtca agcagcccgg
     2821 ccttgcgccg ggcttcttcc gcattggggc cgatcgcata gggatcgatc gccagggcaa
     2881 gcgtctgggc aagggcttcg ccgaggtcga acaagctgcc agtaaactcg atctcgtcga
     2941 gttccgcttc ttccagttcg atttcctcgt ccggggatga cggcgccccg gcgggcacga
     3001 agtgaaggct gacgggctcg tcgatgctga ccggcaggtc ctcgccgctc acggcgcagc
     3061 tctggacaat cgcggcggtc agccggcctt tggcgctggc gccgttcgct tcccgctcga
     3121 ggtcgaccgt ggcggagagc cggtcgatgc ggaccagccc gaaccgcttg gcgagcgcgg
     3181 cgcactcggc ctcggtggct gcgagttcca ccgcgctgcc gggaagcagg cgcggatcat
     3241 aggtgcggct gaattcgctc a
//
LOCUS       DASUDV010000510         8520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_216199,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000510 DASUDV010000000
VERSION     DASUDV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216199"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..382
                     /locus_tag="VFV30_11005"
     CDS             191..382
                     /locus_tag="VFV30_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848663.1"
                     /translation="MRRSLVVTDETARHPFLASLPPHIRRGEEPVRIKRPKWLRAEDV
                     RQFLTAYCACFLAVSLFIA"
     gene            complement(383..1705)
                     /locus_tag="VFV30_11010"
     CDS             complement(383..1705)
                     /locus_tag="VFV30_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848664.1"
                     /translation="MTTQTHLIRSRRFLPLFVTQLLNAFNDNLYRNAMVFFVVYTLYN
                     SEEAEGLFSAVASALFIAPFFLLSALAGQLADMRDKARIIRIVKASEIGIMLVGGAGL
                     LLAWQGVLSQTFTVGLMLLALLAMGIHSTFFGPIKYAILPQHLHENEVLAGTGLVEAG
                     TYIAILSGTILAGWIPVEWAAAGVVLTAVIGYGTAQFVPPAPPQTADQPLDFHVWRSS
                     VTLIKHTMKDQRVFLAIMAISFFWTIGAVLFIQFPPLAKNVLSASKEVASLFLVIFSI
                     GVAIGSVSVNALLKGTVSARYSPVSVLVMGIFVVAFWWVCDSWYAHPQDGLLDSAGFL
                     SEPLAIPLLLSLLGIAVAGGMFVVPLYAFLTTFVDKSETARTIAANNIVNSGAMVLGS
                     VLALGMSAAGVPMVDQLLLGAAMCLISAWLGRKLVAAERAAVSPSGAR"
     gene            1816..2409
                     /gene="pgsA"
                     /locus_tag="VFV30_11015"
     CDS             1816..2409
                     /gene="pgsA"
                     /locus_tag="VFV30_11015"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX4848665.1"
                     /translation="MMSLPNILTLSRIVTLPLLAFLLWWPEWQAGYLMAFALYCLMGI
                     TDYFDGYLARSSGAVSRLGQFLDPIADKIMVATVILVLAAQGVLRGPYVGDMHVIAGL
                     VILAREIAVSGLREFLGGLQVSVPVSRLAKWKTTFQMVCLGALILGKALPWWMISIGG
                     LDMNVPHTVGLTTLWGAAVLTVITGWDYLRVGLKHMD"
     gene            complement(2438..2782)
                     /locus_tag="VFV30_11020"
     CDS             complement(2438..2782)
                     /locus_tag="VFV30_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848666.1"
                     /translation="MKKLALSLALAAGIAATPTLAEELAPVPSSLMNRNGVVCVKVTT
                     AGKVSEVFVVRSTGDGAADADLVDWVRTLSWPKAPASDTSRNTWQPLPVAMGKAPVPD
                     VPETCSPPIARR"
     gene            complement(2823..3719)
                     /locus_tag="VFV30_11025"
     CDS             complement(2823..3719)
                     /locus_tag="VFV30_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX4848667.1"
                     /translation="MTTYLPTLKQLQYLVALHEHGHFGRAADACFVSQSTLSAGLRDL
                     ETLLGVVLVERTKRAVRFTPLGNAVVAKAHRLLREAEELSDMVQSAGQPLSGEMRMSV
                     IPTIAPFLLPRMLPRLRRERPQLKLFLREETSQAAMESLHHGRADCVLLALPFPTGEV
                     EKETIELDALWVAFPKDDPRDPPEEVSPDVIDENRLLLLEDGHCLKEHALAACNRPEL
                     RASATMIGTSLHTLVQMVDNGLGLTMLPEMALDAGILNGTQVVARPLISASANREIAL
                     VWRKNSPRADEFRLLAEELRAG"
     gene            complement(3752..4114)
                     /locus_tag="VFV30_11030"
     CDS             complement(3752..4114)
                     /locus_tag="VFV30_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEX4848668.1"
                     /translation="MSRYMLMPALFLLSAAPALAYGAIAVDKNDSSIEPAYGYSINQP
                     DRATAEQVAVEYCRQYANSRANCQPIVWFETCGAYANSPKYFGYGYGATKAAAIEGAR
                     RMCGRDSCIIRVAVCEKR"
     gene            complement(4162..5349)
                     /gene="rnd"
                     /locus_tag="VFV30_11035"
     CDS             complement(4162..5349)
                     /gene="rnd"
                     /locus_tag="VFV30_11035"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX4848669.1"
                     /translation="MQIHPLITTSEALADLCARLSKAEFVCVDTEFMRENTYWPELCL
                     IQIADSHEAAAIDPLARDIDLSPLLDLLVDNEEVLKVFHAGGQDVEIIHNLTQRTPHP
                     IFDTQIAMMAVSQSEQIGYSNLVESWLGLTIDKGARFTDWSRRPLTERQIEYAIGDVT
                     HLAKIFPKLLNRLIKTGRGEWLDVEMEKLADPANYRNDPDLAWQRIKAPGRNPAMLGR
                     LKALAHWRELEAQDKNIPRGRIARDETVADIASHPPKTQADLAKVRGLSAAWKDNEIG
                     RRMMAALEAAKPLTDAELPPRTPRGAPLGKEGALVADLLKLLLKIRSREIDVASRLLA
                     RSEDLELLAAGVRELPMLSGWRYGVFGKDALDLVEGKTAFAVVNGKLKMSSVVVAEEA
                     EAS"
     gene            5528..6397
                     /locus_tag="VFV30_11040"
     CDS             5528..6397
                     /locus_tag="VFV30_11040"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX4848670.1"
                     /translation="MAYSGPIESWSDQAIFPADQRERLAAAFPLAPVRLRHGLAGHPL
                     LSLDMLALAAERMEPAHVECRQSRNANGAPFAFADQPGPGAAETIRTIASADRWVMLR
                     FAEQLPEYADLLDRLLAEIAPVTARFGPALRPRAFIFISSPGTLTPFHFDPEFNILFQ
                     IAGEKRFATYPPKAPWLTAEQQAAFHRSGDNLLDWQPAFAAEGTVHRLEPGDALYVPY
                     KAPHWVEAGGEASVSLSLTWSCPATLELEAAWQGHALLRARGLPSLTPPDFPGRAPLR
                     ALMRGAAGRLGLG"
     gene            6403..7578
                     /locus_tag="VFV30_11045"
     CDS             6403..7578
                     /locus_tag="VFV30_11045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848671.1"
                     /translation="MVEFVLFEQFEAAMAAAGQVMDFGRRTGAGLRLIRPDRLESADL
                     AAWNDLAAAAQTPSAYAQPWFLLPALRHCDPAQGARLAVVEQADGSWAGVLPIARSAT
                     HGRSPLPNWHSWRHPNQFVSAPLVRTGCAVQFWGSLLEGLDERGGGELALCLADLPLD
                     DPASDALFTLCAEENRTVVVDRRFERAALRGSGSAEPAPKLRRRLASLLRKLERERGA
                     PQFTLIRDRAGIAAAVEQFLALEHSGWKGSCGSALACAPGTMALFADIMREGGQAGRV
                     ELASLRAGGEVLAFSTILVGEGRKYGFKMAYDERAAAYAPGLLLLNWITRSHQDSAAG
                     LDFDSCSAPDQQPVSRLWPDRCAFADCRIALGGKVRQGALRAVMAGEEAYSWLKRLA"
     gene            7703..>8520
                     /locus_tag="VFV30_11050"
     CDS             7703..>8520
                     /locus_tag="VFV30_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX4848672.1"
                     /translation="MHLYRTHTCGALRASDVGQTIRLSGWVHRKRDHGGVLFVDLRDH
                     YGLTQIVADVDSPALAVLEACRVESVVTIEGTVKARSESTVNANLPTGAIEVYARAAT
                     VLSRAEELPLPVAGEQEYPEDVRLKYRFLDLRRETLHANIVKRTQIIRETRRRMEDIG
                     FTEYSTPILTASSPEGARDFLVPSRIHAGKFFALPQAPQQYKQLLMVAGFDRYFQIAP
                     CFRDEDPRADRLPGEFYQLDLEMSFVTQEEVWDTMEPVMAGVFEAFADGKSVTKA"
BASE COUNT         1308 a         2842 c         2920 g         1450 t
ORIGIN      
        1 gacgagcagc cgcccgccgt taactgtcgt gtgtgaggtc atgcgcgggc tttagcgagc
       61 ggcgcgcggt ggggcaacgc cgccgggtcc gataacccac cgcgtggtta ctgtcccaaa
      121 ccgggaagcg ggccgttaac ctcgattttc cggctggtgt gatggtcaac aggttcttaa
      181 cgctgcggct atgagaaggt cgcttgtagt caccgatgag accgcccggc atccgttcct
      241 ggcgtcgctc ccgccccaca tccgccgcgg cgaggagccg gtgcggatca agcgcccgaa
      301 gtggctgcgg gcggaggatg tccgccagtt cctgaccgcc tattgcgcgt gcttcctggc
      361 cgtgagcctg tttatcgcct gatcagcgcg cgcccgatgg gctgacggcg gcacgctcgg
      421 ccgcgaccag cttgcggccc agccaggccg aaatcaggca catggccgcg cccaacagca
      481 gctggtccac catcggcacg ccggcggcgc tcatccccag ggcgaggacc gagccgagca
      541 ccatcgcccc cgagttcacg atgttgttcg ccgcgatcgt ccgcgcggtc tccgacttgt
      601 cgacgaaggt cgtcaggaag gcatagagcg gaaccacgaa catcccgcca gccacggcaa
      661 tccccagcag gctgagcagc agcgggatcg ccagaggttc gctcaggaag ccggcggaat
      721 cgagcaggcc atcctgcggg tgcgcgtacc aggagtcgca aacccaccag aaggcgacca
      781 cgaaaatccc catgacgaga acggaaaccg gcgaatagcg cgccgagacg gtgcccttta
      841 gtaaggcgtt gaccgagacc gagccgatcg cgacgccgat cgagaagatc accaggaaca
      901 ggctggcgac ctccttgctg gccgagagca cgttcttggc cagcggcggg aactggatga
      961 acaggaccgc gccgatcgtc cagaagaagc tgatcgccat gatcgccagg aacacgcgct
     1021 gatccttcat ggtgtgcttg atcagcgtga cggacgagcg ccagacatga aaatcgagcg
     1081 gctggtcagc ggtctgcggc ggggccgggg ggacgaactg ggcggtgccg taaccgatga
     1141 cagcggtcag cacgaccccg gccgcggccc attccaccgg gatccagccg gccaggatcg
     1201 tgccggacag gatcgcgata taggtgccgg cctcgaccag gccggtgccg gccagcacct
     1261 cgttctcgtg caggtgctgc ggcaagatgg cgtacttgat cgggccgaag aaggtggaat
     1321 ggatgcccat ggccagcagg gccagcagca tcaggccaac ggtgaacgtc tggctgagca
     1381 cgccctgcca ggccagtagc aggccggcgc cgcccaccag catgatcccg atttccgatg
     1441 ccttgacgat ccggatgatc cgggccttgt cgcgcatgtc ggccagctgc ccggccaggg
     1501 ccgaaagcag gaagaacggc gcgataaaca gggccgaggc caccgcgctg aacagccctt
     1561 cggcttcctc cgaattgtac agtgtataga caacgaagaa gaccatcgca ttgcgataga
     1621 ggttgtcgtt gaatgcattg agcaattggg tgacgaacag gggcaggaag cgccggctgc
     1681 ggatcaggtg ggtttgggtt gtcatgaact cgcttcagct atcaccggtt ggcgcggacc
     1741 ctagcggctg atcccttgcg gacaagctca atctggccct tttgcccgaa ccgcatcgcc
     1801 gctattcgtt gcaccatgat gtcactgccc aacatcctga cgctgtcgcg gatcgtgacg
     1861 ctgcccttgc tggcgttcct gctgtggtgg ccagagtggc aggccggtta cctgatggcc
     1921 ttcgcgcttt actgcctgat gggcatcact gactatttcg acggctacct cgcccgatcg
     1981 agcggcgcgg tcagccggct tggccagttt ctcgatccga ttgccgacaa gatcatggtg
     2041 gcaaccgtga tcctggtcct ggccgcccag ggcgtgctgc gcgggcccta tgtgggcgac
     2101 atgcacgtga tcgccggcct ggtgatcctg gcgcgcgaga tcgcggtatc ggggctgcgc
     2161 gagtttctgg gcgggctgca ggtttcggtc ccggtcagcc gcctggccaa atggaagacc
     2221 acgttccaga tggtctgcct gggcgcgctg atcctgggca aggcgctgcc gtggtggatg
     2281 atctcgatcg gcgggctgga catgaacgtg ccgcacacgg tcggcctgac cacgctgtgg
     2341 ggcgcggcgg tgctgaccgt gattaccggc tgggattacc tgcgggtcgg cctcaagcac
     2401 atggattgag gccccaaggg cctgttgccg gcgcggccta gcgccgggcg atcggcggag
     2461 agcaggtttc cggaacgtcc ggcaccgggg ccttgcccat cgccaccggc agcggctgcc
     2521 aggtgttgcg gctggtgtcg ctggccggcg ccttgggcca gctgagcgtg cggacccagt
     2581 cgaccagatc ggcgtcggcc gcgccatcgc cggtcgagcg gaccacgaag acttcgctga
     2641 cctttccggc cgtggtcacc ttcacgcaga ccacgccgtt gcggttcatc aggctggacg
     2701 gaacgggggc gagctcctcc gccagggtcg gagtcgcggc gatgccggcg gccagcgcca
     2761 gcgaaagggc aagcttcttc atgggcctga ttgtgccacg cctgcaccgc tgctgcaatg
     2821 gcctagcccg cccgcagctc ctcggcgagc aggcggaact cgtccgcgcg ggggctgttc
     2881 ttgcgccaga ccagcgcgat ttcgcggttg gcgctggcgc tgatcagcgg ccgggcgacc
     2941 acctgggtgc cgttgaggat cccggcgtcg agcgccattt ccggcagcat cgtcaggccc
     3001 aggccgttgt ccaccatctg caccagggtg tgcaggctgg tcccgatcat cgttgccgag
     3061 gcgcgcagtt ccgggcggtt gcaggcggcc agcgcatgct ctttcaggca gtgcccgtct
     3121 tccagcagca gcaggcggtt ctcgtcgatc acgtcgggcg agacttcctc gggcggatcg
     3181 cgcggatcgt ccttgggaaa ggcgacccac agcgcgtcga gctcgatcgt ttccttctcc
     3241 acctcgccgg tcggaaaggg cagggccagc aggacgcagt cggcgcggcc gtggtgcagc
     3301 gattccatcg ccgcctggct ggtctcctcg cgcaggaaca gcttcagctg cgggcgctcg
     3361 cgccgcaacc gcggcagcat ccggggcagc aggaacggcg cgatcgtggg gatcacgctc
     3421 atccgcattt cgcccgacag cggctggccg gcggactgga ccatgtccga cagttcctcc
     3481 gcctcgcgca gcaggcggtg cgccttggca accaccgcgt tgcccagcgg ggtgaaccgc
     3541 accgcgcgct tggtccgttc gaccagcacc acccccagca gcgtttccag atcgcgcagg
     3601 ccggcagaga gcgtcgactg cgagacgaaa caggcgtcgg ccgcacggcc gaaatggccg
     3661 tgctcgtgca gcgcgacaag gtattgcagc tgcttgaggg tgggtaggta ggtggtcatc
     3721 gtggcccttc agccggcgca gggtgcgccg cctatcgctt ctcgcaaacg gcgacgcgga
     3781 tgatgcagga gtcgcgcccg cacatccggc gggccccctc gatcgcggct gccttcgtcg
     3841 cgccatagcc gtagccaaag tatttggggc tgttggcata ggcgccgcag gtttcgaacc
     3901 agacgatcgg ctggcagttg gcacgcgaat tggcgtactg gcggcaatat tccacggcca
     3961 cctgctcggc cgttgcgcgg tccggctggt tgattgagta gccataggcc ggttcgatcg
     4021 agctgtcgtt cttgtccacg gcgattgcgc cataggccaa ggcgggcgcc gcgctgagaa
     4081 ggaacagggc cggcatcagc atgtaacggg acataggctt tccccctggt atcgcacccg
     4141 gtcggcccgg gcgaagcgag gctagctggc ctccgcctct tcagcaacca cgacgctgga
     4201 catcttgagc ttgccgttca ccacggcaaa ggccgtcttg ccttcaacca ggtccagcgc
     4261 gtccttgccg aacacgccgt agcgccagcc gctcagcatc ggcagttcgc gcacgccggc
     4321 ggccagcagt tccaggtcct cgctgcgggc cagcaggcgc gaggcgacgt cgatctcgcg
     4381 gctgcggatc ttgagcagca gcttgagcag atcggccacc agcgcgcctt ccttgcccag
     4441 cggggcgccg cgcggggtgc gcgggggcag ttccgcatcg gtgagcggct tggccgcttc
     4501 cagcgcggcc atcatccgcc ggccgatctc gttgtccttc cacgccgccg aaaggccgcg
     4561 gaccttggcg aggtcggcct gggtcttggg cgggtggctc gcaatgtcgg ccacggtctc
     4621 gtcacgggcg atccggccgc gcgggatgtt cttgtcctgc gcttccagtt cgcgccagtg
     4681 ggccagcgcc ttgagccgcc ccagcatcgc cgggttgcgc ccgggggcct tgatccgctg
     4741 ccaggccagg tccggatcgt tgcggtaatt ggccgggtcg gccagctttt ccatctcgac
     4801 gtcgagccat tcgccgcgcc cggtcttgat cagacggttg agcagcttgg ggaagatctt
     4861 ggcgagatgg gtgacgtcgc cgatcgcgta ttcgatctgc cgttcggtca gcgggcggcg
     4921 cgaccagtcg gtaaaccgcg cgcccttgtc gatcgtcagg cccagccagc tttcgaccag
     4981 gttggaatag ccgatctgct cgctctggct cacggccatc atcgcgatct gggtgtcaaa
     5041 gatcgggtgc ggggtccgct gcgtcaggtt gtggatgatt tccacgtcct gcccgccggc
     5101 gtggaacacc ttcagcacct cttcgttgtc gaccagcagg tcgagcagcg gggacagatc
     5161 gatgtcgcgc gccaggggat cgatcgccgc ggcctcgtgg ctgtcggcaa tctggatcag
     5221 gcacagttcg ggccagtagg tgttttcgcg catgaattcg gtgtcgacgc acacgaattc
     5281 ggccttcgag agccgggcgc aaaggtccgc aagtgcttcg gacgtggtga tcaggggatg
     5341 tatctgcatc gttcttttct ggtcttggcc ccgtgcgagg ccgcggcttg gcaaatgcgc
     5401 ggccgtcgcc agcgggccgc ggacccgccc tgcacgattc ggcgccgaat gaaaaggggg
     5461 cgcggcccgc tggaatggcg gcgctgctag ccagttctta agggcttggc gctatcaggc
     5521 ctgcgcgatg gcatattccg gcccgattga atcctggagc gatcaggcga tctttcccgc
     5581 tgaccagcga gagcggctgg ccgcggcctt tccgcttgcg ccggtgcggc tgcgccacgg
     5641 cctggccggg catccgctgc tgtcgctgga catgctggcg ctggcggccg agcggatgga
     5701 gcctgcccac gtcgaatgcc gccagtcgcg caacgccaac ggcgcgccgt ttgcctttgc
     5761 ggaccagccg ggacccggcg cggccgaaac gatccgcacc atcgccagtg ccgaccgctg
     5821 ggtgatgctg cgctttgccg aacaattgcc cgaatacgcc gacctgctgg accgtctgct
     5881 ggccgagatc gcgccggtca ccgcgcggtt cggcccggcg ctgcggccgc gggccttcat
     5941 cttcatttcc tcgcccggca cgctgacgcc gtttcacttc gatccggaat tcaacatcct
     6001 gttccagatc gccggggaaa agcgcttcgc cacctatcct ccgaaggccc cgtggctgac
     6061 cgcggagcag caagccgcgt tccaccggtc gggcgacaac ctgctggact ggcagccggc
     6121 cttcgcggcc gagggcacag tccaccggct ggagccgggc gatgcgctct atgtgcccta
     6181 caaggcgccg cactgggttg aggcgggcgg cgaagcctcg gtctcgctgt cgctgacctg
     6241 gtcgtgcccg gccacgctgg agctggaagc ggcctggcaa ggccacgccc tgctgcgcgc
     6301 gcgcgggctg ccctcgctga cgccgccgga ttttccgggc cgtgcgccct tgcgcgcgct
     6361 gatgcgcggt gccgctggtc ggctggggct ggggtagccg cgatggtcga attcgtcctg
     6421 tttgagcagt tcgaagccgc catggccgcg gccgggcagg tcatggactt tggccggcgg
     6481 accggggccg gcctgcgtct gatccggccg gaccggctgg agagcgccga tctcgccgcg
     6541 tggaacgatc tggccgctgc ggcccagacg cccagcgcct atgcccagcc gtggttcctg
     6601 cttccggccc tgcgccactg cgatcccgcg caaggcgcgc ggctggcggt ggtggaacag
     6661 gccgacggca gctgggccgg ggtcctgccc attgcccgca gcgccacgca cggccgcagc
     6721 ccgctgccca attggcactc gtggcgccac cccaaccagt tcgtctctgc gccgctggtc
     6781 cggaccggct gcgcggtgca gttctggggc tcgctgctgg aagggctgga cgagcgcggc
     6841 ggcggggagc tggccctgtg cctggccgac ttgccgctcg acgatccggc cagcgatgcc
     6901 ctgttcacgc tctgcgccga ggaaaaccgc acggtcgttg tcgaccggcg ctttgaacgc
     6961 gcggccctgc gcgggtccgg ttccgcggag ccggcgccca agctgcgccg ccgcctggcc
     7021 tcgctgctgc gcaagctgga gcgcgagcgc ggcgcgccgc agttcacgct gatccgcgac
     7081 cgggccggaa tcgccgccgc ggtcgagcag ttcctcgcgc tggagcattc cggctggaag
     7141 ggctcctgcg ggtcggccct ggcctgcgcg ccgggcacca tggccctgtt tgccgacatc
     7201 atgcgcgaag gcggccaggc cgggcgggtc gagctggcct cgctccgcgc cgggggcgag
     7261 gtgctggcct tttcgaccat actggtgggc gagggccgca aatacggctt caagatggcc
     7321 tatgacgagc gcgccgcggc ctatgcgccc ggtctcctgc tgctcaactg gatcacccgg
     7381 tcgcaccagg atagcgccgc cggcctcgat ttcgacagct gctccgcgcc ggatcagcag
     7441 ccggtctcgc gcctttggcc cgatcgctgc gcctttgccg attgccggat cgcgctgggc
     7501 ggcaaggttc gccagggcgc gctgcgcgcg gtgatggccg gggaagaggc ctattcctgg
     7561 ctcaagcgac tggcctgagc ggccgggagc gccagcccaa tggtgacagg caagtcttga
     7621 caaagcgaca cgtcacggtt gttagggcgc cattcggcgt cccctgctgg cgcatcccct
     7681 gttttcggaa gctgacggtt tcatgcatct ttatcgcacc catacctgcg gcgcgctgcg
     7741 cgccagcgat gttggccaga cgatccgcct ctcgggctgg gtccaccgca agcgcgacca
     7801 cggcggcgtc ctgttcgtcg acctgcgcga ccattacggc ctgacccaga tcgtcgccga
     7861 cgtggacagc ccggcgctgg cggtccttga agcatgccgt gtggaatcgg tcgtcacgat
     7921 cgagggcacg gtcaaggccc gcagcgaaag tacggtcaac gccaacctgc cgaccggtgc
     7981 gatcgaggtc tatgcccgcg ccgccacggt gctgagccgc gcggaagaac tgccgctgcc
     8041 ggtggccggg gaacaggaat accccgagga cgtgcgcctg aagtaccgct tcctcgacct
     8101 gcgccgcgag acgctgcacg ccaacatcgt caagcgcacc cagatcatcc gcgaaacccg
     8161 ccgccggatg gaggatatcg gctttaccga atattcgacc ccgatcctga cggcgtcctc
     8221 gcccgaaggc gcgcgcgact ttctggtgcc gagccggatc cacgccggca agttctttgc
     8281 cctgccgcag gccccgcagc agtacaagca gctgctgatg gtggcgggct ttgaccgcta
     8341 tttccagatc gccccctgct tccgcgacga agacccgcgc gccgaccggc tgccgggcga
     8401 gttctaccag ctcgacctgg aaatgagctt cgtcacccag gaagaggtct gggacacgat
     8461 ggagccggtc atggccgggg tgttcgaggc ctttgccgac ggcaagagcg tgaccaaggc
//
LOCUS       DASUDV010000511         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_702210,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000511 DASUDV010000000
VERSION     DASUDV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702210"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFV30_11055"
     CDS             <1..292
                     /locus_tag="VFV30_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX4848673.1"
                     /translation="GFYEDALEAAKGAGAHEALFLRDDGLVTEGSFTNLFVERDGKLL
                     TPPAALGLLPGVLRRSLIEEGRAVEAELTLDDLANGFLIGNALRGLVRARLA"
     gene            289..1455
                     /locus_tag="VFV30_11060"
     CDS             289..1455
                     /locus_tag="VFV30_11060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4848674.1"
                     /translation="MKLSQSLSRIAPSRTAAMADRAFELRTAGKDVISLSVGEPDFAA
                     PPHVIAAAQAALDAGDTRYTAVAGTPRLREAAALHFARDLGIEVPPAQVMISAGGKQA
                     VFHALCATLDPGDEVLIPSPWWVSYPEIVRFTGASVIDLPTTPATGYRIDAAQLAAAI
                     TPNTRWLLLNSPGNPTGSVYSAQDLRALGAVLEKHPQIMVLSDDIYAPLRYLPGRHAT
                     IAAECPELAERVLTVSGVSKSHAMMGFRIGLAAGPEWLIKAMTRLQSHSSGNPASISQ
                     AAAVAALEGPQDFLIDWCERFRVRRDLVVAAINAIPGLSCPVPDGAFYCLVDASPHMA
                     RFGDDEALCLHLLDHGVAVVAASAFGGSNGFRISFAADEAKLEEALRRIAAALA"
     gene            1452..2120
                     /locus_tag="VFV30_11065"
     CDS             1452..2120
                     /locus_tag="VFV30_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX4848675.1"
                     /translation="MSEIPILFEDGEALVIDKPAGLPIERPRAGGRSLEDHLDALKLG
                     FQRAPLPVHRIDTDTSGCLLLARNPKALKRFNAAFEARSVDKVYLGVVAGPVAGEEGT
                     IELALAKVSSAEKGWRMIPAKKGKPAVTHWRKLAERDGLTLVEFRPETGRTHQIRVHA
                     LAGLGHALLGDPVYGSGTGAARTMLHARALDVTREGKPPISALAPIPADFAALGFGDV
                     DQRG"
     gene            2101..>2327
                     /locus_tag="VFV30_11070"
     CDS             2101..>2327
                     /locus_tag="VFV30_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX4848676.1"
                     /translation="MSTSADEATARALALASESFIAASGPGGQNVNKVASAVQLRLNV
                     YALALPVPVFARFKQLAGSRMTARGELVLTA"
BASE COUNT          346 a          792 c          814 g          375 t
ORIGIN      
        1 cggcttttac gaggacgcgc tcgaggccgc gaagggtgcc ggcgcgcacg aggcgctgtt
       61 tctgcgcgac gatggcctcg tcaccgaagg cagcttcacc aacctgttcg tcgagcgcga
      121 cggcaagctc ttgaccccgc ctgccgcgct ggggctgctg ccgggcgtgc tgcgccgttc
      181 gctgatcgag gagggccgcg cggtggaggc cgagctgacg cttgacgatc tggccaatgg
      241 cttcctgatc ggcaatgccc tgcgcgggct ggttcgggcg cggctggcat gaagctcagc
      301 cagtcgcttt cgcggatcgc gccttcccgc accgcggcca tggccgaccg tgccttcgaa
      361 ctgcgcaccg ccggcaagga cgtgatctcg ctgtcggtgg gcgaacccga tttcgccgca
      421 ccgccgcacg tgatcgccgc agcccaggcc gcgctcgatg cgggcgatac ccgctatacc
      481 gcagtggccg gcaccccgcg cctgcgcgaa gccgccgcgc tgcatttcgc gcgcgatctg
      541 gggatcgagg tgccgcccgc gcaggtgatg atcagcgccg ggggcaagca ggcggtgttc
      601 catgcactgt gcgccacgct cgacccgggc gacgaggtgc tgatcccttc gccgtggtgg
      661 gtttcctatc ccgaaatcgt gcgcttcacc ggggccagcg tgatcgacct gccgaccacg
      721 cccgccaccg gctaccggat cgatgccgcg cagcttgccg cggcgatcac gcccaacacc
      781 cgctggctgc tgctcaacag tccgggcaac ccgaccggtt cggtctattc ggcccaggat
      841 ttgcgcgcgc tgggcgcggt gctcgaaaag catccccaga tcatggtgct gagcgacgat
      901 atctatgcgc cgctgcgcta cctgcccggt cgccacgcga cgatcgcggc cgaatgcccg
      961 gagctcgccg aacgcgtgct gaccgtttcg ggcgtatcga aaagccacgc gatgatgggc
     1021 ttccggatcg gcctggcggc gggtcccgaa tggctgatca aggcgatgac ccggctgcaa
     1081 tcgcactcct cgggcaatcc cgcctcgatc agccaggccg cggcggtggc tgcgctcgaa
     1141 gggccgcagg acttcctgat cgactggtgc gaaaggttcc gcgtgcggcg cgatctggtg
     1201 gtggcggcga tcaacgccat tcccgggctg tcgtgccccg tgcccgatgg cgccttctat
     1261 tgcctggtcg atgcgagccc gcacatggcg cggttcggcg atgacgaggc gctgtgcctg
     1321 cacctgctcg atcacggggt ggcggtggtg gcggcatcgg cgttcggcgg cagcaacggc
     1381 ttcaggatca gcttcgccgc cgacgaggcg aagctggaag aggcgctgcg ccggatcgcg
     1441 gcggccctgg catgagcgaa attccaatcc tgttcgaaga cggcgaggcg ctggtgatcg
     1501 acaagccggc gggcctgccg atcgagcgcc cgcgcgccgg ggggcgcagc cttgaagatc
     1561 atctcgatgc gctgaaactc ggcttccagc gcgcgccgtt gccggttcac cggatcgata
     1621 ccgatacttc gggctgcctg ctgctggcgc gcaatcccaa ggccctgaag cgcttcaacg
     1681 ccgcgttcga ggcgcgcagc gtcgacaagg tctatctcgg cgtggtggcc ggcccggttg
     1741 cgggcgagga aggcacgatc gaactggcgc tggccaaggt ttccagcgcc gaaaagggct
     1801 ggcggatgat cccagccaaa aagggcaagc ccgcggtgac ccactggcgc aagctggccg
     1861 aaagggacgg gctgaccctg gtcgagttcc gccccgaaac cggccgcacg caccagatcc
     1921 gggttcatgc gctcgccggg cttggccatg ccctgctggg cgatccggtc tatggcagcg
     1981 gaaccggcgc ggcgcggacc atgctccatg cccgcgcgct ggatgttacc cgcgagggca
     2041 agccgccgat cagcgccctt gccccgatcc cggccgattt cgcggcgctt ggctttggcg
     2101 atgtcgacca gcgcggatga agccacggcc cgcgcgctgg cgctggcaag cgaaagcttc
     2161 atcgctgcct cgggtccggg cggtcagaac gtcaacaagg tggcaagcgc ggtgcagctg
     2221 cggctcaacg tctatgcgct ggccctgccg gtgccggttt ttgcccggtt caagcagctg
     2281 gccggcagcc ggatgaccgc gaggggcgag ctggtcctca ccgcgca
//
LOCUS       DASUDV010000512         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1210284,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000512 DASUDV010000000
VERSION     DASUDV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210284"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFV30_11075"
     CDS             <1..185
                     /locus_tag="VFV30_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848677.1"
                     /translation="KYPYSGGYDTIAYHAIDRIAMEYPGAADARALLDYLRTEKGVDV
                     DARWAEYRRNRARSKD"
     gene            complement(195..851)
                     /locus_tag="VFV30_11080"
     CDS             complement(195..851)
                     /locus_tag="VFV30_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848678.1"
                     /translation="MRGLILAGALAALLAAPAQAEDPQRVCSGHAAGANGEAVNVMIY
                     LTAKGDRVGSFASWDPPRIGPPAPTAEAVPDVYFSMMYHQAGPSGLGPAGDPAIGATA
                     FAPPGKRGRGNAERQFAGLSVELTVDSSAPQMLPLTVNALFAPLPMTAMREAKAPALP
                     ANAGTVTLRLVRSGKPPLSTARYDLSGTAERDRLFTAAWQNAETAARTPETCEETVAP
                     "
     gene            complement(851..1357)
                     /locus_tag="VFV30_11085"
     CDS             complement(851..1357)
                     /locus_tag="VFV30_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4848679.1"
                     /translation="MSFSTMRPWLAGGAMIVAVAGAAQAQEDPKAAFEARYVELRDAM
                     MGNNKAAAERLLAADYEIIDIRGETRGRAEVMERMGQMPPGMENVKPESKVLKVTVKG
                     DTANVESQMTMQMKRPDENGEEMLLDIAVISDDVWVQRGGAWVLQRSTQKELTVSRNG
                     EVVFRQAN"
     gene            complement(1571..2140)
                     /locus_tag="VFV30_11090"
     CDS             complement(1571..2140)
                     /locus_tag="VFV30_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX4848680.1"
                     /translation="MTPRFTGRPLAFALAALAGTASLAALPPAAIAGHHGSHMNAPAT
                     VVDIAVGSPDHKTLVAAVKAAGLVETLQGTGPFTVFAPTDAAFAKLPAGTVETLVQPQ
                     NKPVLTKVLTYHVVAGKVTSADLVALIRKGKGKAVVTTVAGETLTARLHGKTIVITDG
                     KGRKSVVTAANIKAGNGVVHVVDGVFLPR"
     gene            complement(2228..2956)
                     /locus_tag="VFV30_11095"
     CDS             complement(2228..2956)
                     /locus_tag="VFV30_11095"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX4848681.1"
                     /translation="MTERELFAAELALRLLSDEEHAAARAELERDPELAALVAQWEGR
                     LAPLANALEAVPPSPQTWQRIAASLEPARADSTADNDNDRHEADRLRARLRRWQWGSA
                     AVAAAALVLGIVGAPLLQRGPALSDPAAPVLAASLPVGSATGPRLALTFLPASGDLLV
                     EASSVPGDGVHDHELWLVVPDGKPVSMGLITSGTQRRIALPPDLARRVDAGLDVVLTR
                     EPLGGAPKGGAAGPVVATGKFSTI"
     gene            complement(2953..3510)
                     /locus_tag="VFV30_11100"
     CDS             complement(2953..3510)
                     /locus_tag="VFV30_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4848682.1"
                     /translation="MTAADSPDALELALARLAGGDPAGLEQVYRLSAPRVLAICIRIL
                     GDRSEAEDVLHDTYVRLAANAGRFRRGHGSAIGWLVTIARNLSIDRLRSRAVRGVQVP
                     VEAAEALPDKDPDAETRLLRAEARTRLDGCLSALDEAQQGAIRSAFFAGYSYAQLADQ
                     RGVPLGTMKSWVRRGLARLKACLEQ"
     gene            complement(3520..4557)
                     /locus_tag="VFV30_11105"
     CDS             complement(3520..4557)
                     /locus_tag="VFV30_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4848683.1"
                     /translation="MSGSHDHKHGHDHGHGHDHGHGHAHGHHHGHGHHHGHGHGHGHG
                     HGHGHHHHHSHGQGSHGWAFGIAVVLNLTFVVVEVIASFISNSAALLADAGHNLGDVL
                     SLLLAWGASILASRPAGPGYTYGLKSSSILAAIANAALLWVALGAVLLETLQRLWDPG
                     EVQGLQVMAVAGVGILVNLASALLFAKGARNDLNLRAAFQHLIADAVVSASVVFAGLV
                     IALSGQHWIDPVTSLIITVGLAWGSWDLLKDAVKMGLLAVPRHIDETAVRAFLAEQDG
                     VTAVHDLHIWGMSTTESALTAHLVLPAGHPGDDFLRALAEQLEARFGIHHATIQIEQS
                     AADCRLHGKPA"
BASE COUNT          724 a         1643 c         1687 g          738 t
ORIGIN      
        1 gcaagtaccc ctatagcggc ggctacgaca ccatcgccta ccacgcgatc gaccgcatcg
       61 cgatggagta ccccggcgca gcggacgcgc gggcgttgct ggactatctg cgcaccgaaa
      121 agggcgtcga tgtcgatgcc cgctgggcgg aataccgccg caatcgcgcg cgcagcaaag
      181 actaggcggc gcgcctaggg cgcaactgtc tcttcgcagg tctctggcgt gcgcgcggcc
      241 gtctcggcgt tttgccaggc ggcggtgaac aggcggtcgc gttcggcggt gccgctcagg
      301 tcatagcggg cggtcgaaag gggcggcttg ccgctgcgga ccagccgcag ggtgacggtt
      361 ccggcgttcg cgggcagggc tggggccttg gcctcacgca ttgcggtcat gggcaagggc
      421 gcgaacagcg cgttgacggt cagcggcagc atctgcgggg cgctgctgtc gaccgtcagt
      481 tcaacgctca gcccggcaaa ctgccgttcc gcattgccgc ggccgcgctt gcctgggggg
      541 gcaaaggcgg tggccccgat cgccggatcg ccggccggcc caaggccgga tggaccggcc
      601 tgatggtaca tcatcgaaaa atagacgtcc ggcaccgcct cggccgtggg ggcgggcggg
      661 ccgatccggg gcgggtccca gctggcgaag ctgcccaccc gatcaccctt ggcggtcagg
      721 tagatcatca cgttgaccgc ttcgccgttg gcgccggcgg catggccgct gcagacccgc
      781 tgcggatcct cagcctgcgc gggggccgcc agcaatgccg ccagagcccc cgccaggatc
      841 agtccgcgca tcagttggcc tgacggaaca ccacttcgcc gttgcgcgaa acggtcagct
      901 ctttctgggt gctgcgctgc aggacccagg ccccgccgcg ctggacccag acgtcgtccg
      961 agatcaccgc gatgtcgagc agcatttcct cgccgttttc gtccgggcgc ttcatctgca
     1021 tggtcatctg gctttcgacg ttggcggtgt cgcccttgac cgtcaccttc agcaccttgc
     1081 tttcgggctt cacgttttcc atgcccgggg gcatctggcc catccgctcc atcacttcgg
     1141 cgcggccgcg ggtttcgccg cggatgtcga tgatttcata gtcggccgcc agcagccgtt
     1201 cggccgctgc cttgttgttg cccatcatgg catcgcgcag ctcgacatag cgggcctcga
     1261 aggccgcctt gggatcttcc tgggcctggg ccgcaccggc caccgccaca atcatcgcgc
     1321 cgccggccag ccaggggcgc atcgtcgaaa agctcatcgg gtccactcct tggaaagttc
     1381 gcagcggttt agcggcttgc ttgccaaccg gagatgaacg gatcgaccaa gggctgctgc
     1441 catccgccga acggcagacc ggcgccggta tggtcccgtc cggggcggga gggggttggg
     1501 tcggaccccg gacgggaccg gtgccgccgg ccgaggcgtg gccggcggca cggatctgcg
     1561 ggcggcagaa tcagcgcggc aggaacacgc cgtcgaccac gtggacaacg ccgttgccgg
     1621 ccttgatgtt ggcggcggtg acgaccgact tgcggccctt gccatcggtg atgacgatgg
     1681 tcttgccgtg cagccgcgcg gtcagcgttt caccggcgac cgtggtgacc acggccttgc
     1741 ccttgccctt gcggatcagc gcgacgagat cggcggaggt gaccttgccg gccaccacgt
     1801 gataggtcag caccttggtc agcaccggct tgttctgcgg ctggaccagg gtttcaaccg
     1861 tgccggcggg cagcttggca aaggcggcat cggtcggcgc gaagacggtg aacgggccgg
     1921 tgccctgcag cgtttcgacc agaccggcgg ccttgaccgc ggcgaccagg gtcttgtgat
     1981 cgggcgagcc gaccgcgatg tcgaccacgg tggcgggggc gttcatgtgc gagccgtgat
     2041 ggccggcgat ggctgccggg ggcagcgcgg ccagcgaagc ggtgccggcc agcgcggcca
     2101 gggcgaaagc aagcgggcgg ccagtgaagc gaggggtcat ggggtaagct cctgttggaa
     2161 acgcgcaggc gtcacgggcc tgcatcatcc atacggagca agggggcgaa cggatgcacc
     2221 cgcccggtca gatcgttgaa aattttcctg tcgccaccac cgggccggcc gcgccgccct
     2281 tgggcgcgcc gcccagcggc tcgcgggtca gcaccacatc cagcccggca tcgactcgcc
     2341 gggccagatc gggcggcaag gcgatccggc gctgcgtgcc cgacgtgatc aggcccatcg
     2401 acaccggctt gccatccggc acgaccagcc acagttcgtg gtcgtgcacg ccgtcgcccg
     2461 gcacgctgct ggcctcgacc agcagatcgc cgctggcggg caggaaggtc agcgccaggc
     2521 gcgggccggt cgcgctgccg acgggcagac tggcggccag gaccggcgcg gcgggatcgg
     2581 acagcgccgg accgcgctgc agcagcggag cgcccacgat gcccagcacc agcgccgcgg
     2641 ccgccacggc ggcgctgccc cattgccagc gccgcaaccg ggcgcgcagc cggtcggcct
     2701 cgtgccggtc gttgtcgttg tcggcggtgc tgtccgcgcg cgccggttcc agtgaggccg
     2761 caatccgctg ccaggtctgg ggtgagggcg gcacggcttc cagcgcgttc gccagcgggg
     2821 ccaggcggcc ttcccattgc gccaccagcg cggccagttc cggatcgcgc tccagctcgg
     2881 cgcgcgcggc cgcgtgctct tcgtccgaga gcagccgcag cgccagttcg gcggcaaaga
     2941 gttcgcgctc ggtcattgtt cgaggcaggc cttcagccgc gccaggccgc ggcggaccca
     3001 gcttttcatc gtccccagcg gcacgccgcg ctggtcggcc agctgggcat agctgtagcc
     3061 ggcaaagaag gccgagcgga tcgcgccctg ctgggcctcg tccagcgccg aaaggcaccc
     3121 gtcaaggcgg gtgcgggctt cggcccgcag caggcgggtc tcggcgtccg gatccttgtc
     3181 gggcagcgcc tcggccgctt cgaccggcac ctgcaccccg cgcaccgcgc ggctccgcag
     3241 ccggtcgatc gaaaggttgc gggcgatggt gaccagccag ccgatcgccg aaccgtgccc
     3301 gcggcggaat cggccggcgt tggcggccag ccgcacatag gtgtcgtgga gcacgtcctc
     3361 cgcctcgctc cggtcgccca ggatgcggat gcagatcgcc aggacccgcg gcgcggacag
     3421 ccggtagacc tgctcaagcc cagcggggtc gccgccggcc agccgggcca gcgccagttc
     3481 cagcgcatcg ggagaatcgg cggcggtcat tgcgccggac tatgccggtt tgccgtgcag
     3541 gcggcaatcg gccgccgact gttcgatctg gatcgtggcg tggtggatgc cgaagcgcgc
     3601 ctcaagctgc tcggccagcg cgcgcaggaa atcgtcgccg gggtgtccgg cgggcagcac
     3661 cagatgggcg gtcagcgcgc tttcggtggt gctcatcccc cagatgtgca gatcgtgcac
     3721 cgcggtcacc ccgtcctgtt cggccaggaa ggcccgcacc gcggtctcgt cgatgtggcg
     3781 gggcactgcc agcaggccca tcttgaccgc gtccttgagg aggtcccagc tgccccaggc
     3841 caggcccacg gtgatgatca ggctggtgac cggatcgatc cagtgctggc ccgacagggc
     3901 gatcaccagc ccggcgaaga cgacgctggc cgaaaccacc gcatcggcga tcaggtgctg
     3961 aaaggccgcg cgcaggttga ggtcgtttct cgcgcctttg gcaaacagca gcgccgaagc
     4021 caggttaacc agaatgccga ccccggccac ggccatcacc tgcaacccct ggacctcgcc
     4081 gggatcccac agccgctgca gcgtttcgag cagcaccgcc cccagcgcca cccacagcag
     4141 cgcggcgttg gcgatcgcgg ccaggatcga gctggacttg aggccatagg tatagcccgg
     4201 cccggccgga cgcgaggcga ggatcgaggc gccccaggcc agcagcagcg acaggacatc
     4261 gcccagattg tgcccggcat cggccagcag cgcggcggaa ttgctgatga agctggcaat
     4321 gacctcaacc accacgaagg tcaggttgag caccaccgca atgccaaagg cccagccgtg
     4381 gctgccctgg ccgtggctgt gatggtggtg atggccatga ccgtgcccat ggccgtggcc
     4441 atgcccgtgg ccatggtggt gtccatgccc atggtgatgg ccatgcgcgt gcccgtgccc
     4501 atgatcgtgc ccgtgcccat gatcgtggcc gtgtttatgg tcgtgcgaac cgctcatcgg
     4561 ccctcctttc cgcaggcgtt gcacaaatgc cgcagtgcag caagaatcgt cacaccaagg
     4621 gcggtggatt aaccagcccc gccaaccgta tatggcggag ccgcgaacga tccttgcgtg
     4681 cttttgcgga tgcttgaaac gtcattgcgc aaggccgaag cggtccggaa ggccccgcgg
     4741 attctgccac aggcttgtca cattgggctg tttagcgtgg cttcgcgctc ga
//
LOCUS       DASUDV010000513         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1674248,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000513 DASUDV010000000
VERSION     DASUDV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674248"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFV30_11110"
     CDS             complement(<1..294)
                     /locus_tag="VFV30_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEX4848684.1"
                     /translation="MPGRFITYLDSIRARDPAVRSRWEILLYPGVWALGFHRIAHRLF
                     RARLYLLARLVNHFSRFMTAIDIHPGAKIGRNFFIDHGFVVIGETAEIGDNVTM"
     gene            complement(336..950)
                     /locus_tag="VFV30_11115"
     CDS             complement(336..950)
                     /locus_tag="VFV30_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX4848685.1"
                     /translation="MSQIALPLHAGGAGPGRIVVGNANATALEACAVAETWPFRTAVL
                     TGPPRSGKSLIARWFVETGAGEAIDDADRIDETELFHRWNRAQESGTPLLLVRSATPG
                     GWQIALPDLRSRLGAVLQLEIGAPDDAMLAELLAVHAEQRLLALGPDAVTYLVPRIER
                     THMGAEQVIAAIDRLSLERKAPPGPAIWRAALEAVLGPDEPRLL"
     gene            complement(950..2224)
                     /locus_tag="VFV30_11120"
     CDS             complement(950..2224)
                     /locus_tag="VFV30_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX4848686.1"
                     /translation="MAVASLPSLPLRWRPGPAATLLSGLGAALVLALAVIGPQRLIAQ
                     IEGDRGITPVASSSDIAITGVQVETTGDSPEDARLNGWREAQKLAWAKAGGPAMDLGV
                     IESMVSAVVVEREQIGPRRYIATLGVVFDRARAGQYLGGGGAAGAHSAPLLVIPVLRS
                     GGVSQVFEVRGAWQAAWAAFNAGTSPIDYVRPSGAGGDSLLLTAGQPQRRSRAWWRNV
                     LDQFGAADVIIPEARLERQWPGGPVRGTFTARYGPDNRFLESFTLTANDEAGVPAMLA
                     TAVQRIDGIYANALAAGTLKPDATLNHGGQMDAGLAALVEAARRAEQASASMTDATAT
                     PTPSASAAAPATLSTFTVQFVSPDASAVDAALAAVRGAPGVQGASTTSLAMGGTSVMR
                     VTYAGELSGLADALRARGWRVTMGPNALSIRR"
BASE COUNT          390 a          792 c          752 g          322 t
ORIGIN      
        1 catggtgaca ttgtcgccaa tctcggccgt ttcaccgatc accacgaagc cgtgatcgat
       61 aaagaaatta cgcccgatct tcgcgccggg gtggatatcg atcgccgtca tgaaccggct
      121 gaaatggttg accagtcgcg ccagcagata aagccgcgcc cggaacagcc ggtgggcgat
      181 gcggtgaaag ccgagcgccc agaccccggg atacaacagg atttcccagc gcgagcggac
      241 ggccggatcg cgggcccgga ttgaatccag ataggtaatg aaacgtcccg gcataagttc
      301 ttcccatccg ccgcgaatgt cccacagggg ggccttcaaa gcaagcgcgg ctcgtccggt
      361 cccaggaccg cctccagcgc ggcgcgccag atcgcgggac cgggcggcgc cttgcgttca
      421 agactgagcc ggtcgatcgc ggcgatgacc tgctctgctc ccatgtgggt acgttcgatg
      481 cgaggcacaa gataggttac agcatcgggg cccagtgcca gcaaacgctg ctcggcatgg
      541 accgccagca gctcggccag catggcatcg tccggcgcgc cgatttcaag ctggagcacg
      601 gcccccaacc gcgatcgcag gtcaggcagt gcgatctgcc agccgccggg agtggcgctg
      661 cgcaccagca gcagcggcgt gccgctttcc tgcgcccggt tccagcggtg gaacagttcg
      721 gtctcatcga tccggtccgc atcatcgatt gcctcgcccg cgccagtctc gacaaaccag
      781 cgcgcgatca gcgatttgcc agagcgcggc gggccggtga gcacggccgt gcggaacggc
      841 caggtttcag cgacggcgca ggcttccagc gcagtagcat tggcattgcc gacgacaatg
      901 cggcccggcc ccgcccctcc ggcgtgcagc ggcagcgcga tctggctcat cagcggcgga
      961 tcgacagcgc gttggggccc atggtcacgc gccagccgcg cgcgcgcagg gcatcggcca
     1021 ggcccgaaag ctcgcccgca taggtgaccc gcatgaccga ggtgccgccc atcgccaggc
     1081 tggtggtcga agccccttgc acccccggcg ccccgcgcac tgccgcaagg gccgcatcaa
     1141 ccgccgaggc atcgggcgag acgaactgga cggtgaaggt ggacagcgtg gccggcgcgg
     1201 cggccgatgc cgacggcgtt ggggtggccg tagcgtcagt catgcttgcc gacgcctgtt
     1261 ccgcccggcg tgcggcttcg accagcgcag ccaggccggc atccatctgg ccgccatggt
     1321 tgagtgtggc atcgggcttc agcgtgccag ccgccagcgc attggcatag atcccgtcga
     1381 tccgctgcac ggcggtggcc agcatggccg gcaccccggc ttcgtcgttg gcggtcaggg
     1441 taaagctttc gaggaagcgg ttgtccgggc cgtagcgcgc ggtgaaggtt ccgcgcaccg
     1501 ggccaccggg ccattgtcgc tccaaacgcg cttcgggaat gatcacatca gccgcgccga
     1561 actgatcgag cacgttgcgc caccaggcgc ggctgcggcg ctgcggttgc ccggcggtga
     1621 gcagcagcga atcgccgcca gcgccgctcg gccggacgta gtcgatcggg ctggtcccgg
     1681 cattgaacgc cgcccaggcc gcttgccatg ccccacgcac ttcgaaaacc tggctgaccc
     1741 cgcccgaacg cagcaccggg atcaccagca gcggcgccga atgggcgccg gccgcaccgc
     1801 cgccgcccag atattgtccg gcacgggcac ggtcgaacac aacgcccagc gtggcaatgt
     1861 aacgccgcgg gccgatctgc tcacgctcca ccaccacggc cgagaccatg ctttcgatca
     1921 cgcccaggtc catcgccggg ccaccggcct ttgcccaggc cagtttctgc gcctcgcgcc
     1981 agccgttaag ccgcgcgtcc tcgggcgaat cgcccgtggt ctcgacctga acaccggtga
     2041 tcgcaatatc ggacgaggac gcgaccggtg tgatgccgcg atcgccctcg atctgcgcaa
     2101 tcagccgttg cgggccgatc acagccaacg cgagcaccag cgccgccccc agcccgctca
     2161 gcagggtggc ggcagggccg ggacgccagc ggagcggcag ggaaggaagg gatgcgacag
     2221 ccatcgggga aaaccgtgcg ccttttgccc aatcgc
//
LOCUS       DASUDV010000514         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1916995,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000514 DASUDV010000000
VERSION     DASUDV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916995"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFV30_11125"
     CDS             <1..471
                     /locus_tag="VFV30_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX4848687.1"
                     /translation="VVLLGVIGTDVGAYFTGRTLGGPKIAPAISPSKTWSGLIGGMLG
                     AALGIYLAMRIAFTAPFIDAMNAAMSGEPDAVTLVPGGEGPSLVGVLVAGAVLAVVAQ
                     AGDFFESWMKRRAGVKDSGRLIPGHGGLFDRTDGFLAVLFVLGLLSLHSFVFPA"
     gene            468..1625
                     /gene="dxr"
                     /locus_tag="VFV30_11130"
     CDS             468..1625
                     /gene="dxr"
                     /locus_tag="VFV30_11130"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX4848688.1"
                     /translation="MTTRTITILGATGSIGASTLDLVRRDRDAWRVVALTANGNAAEL
                     ARLAREFGAELAVVADEAALPALREALAGSGIEAAGGAAALVEAAARPADMTMAAIVG
                     CAGLAPTMAAIARGQSVALANKEALVSAGDVLMAAVAQSGATLLPTDSEHNAIFQCLA
                     GNDLSQVRSITLTASGGPFRDWAAADLAAATPEQAVKHPNWSMGAKISVDSATMFNKG
                     LELIEAHHLFPVGLDRLKIIVHPQSVVHSMVEYRDGSTLAQLGPSDMRVPIASCLAWP
                     ERMDTPLPPLDLAAIGELTFRAPDEDRFPATRLCRQAAEQGGAAPAVLNAGNEVAVAA
                     FLSGHVAFTRIAAMVEDLMGRYAPAAPVSLGDVIAIDAEARDHARRLLELA"
     gene            1648..2736
                     /gene="rseP"
                     /locus_tag="VFV30_11135"
     CDS             1648..2736
                     /gene="rseP"
                     /locus_tag="VFV30_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX4848689.1"
                     /translation="MLTVGAFLLLLGPLIVLHELGHYWAGRAFGVRAEVFSVGIGKEL
                     WHRIDKRGTRWRFAAIPLGGYVLFAGDADASSRPDPALDGASAERLKGTFPGAPLWQR
                     TLIVLAGPAANLLVAVAIIAAFVAMIGVPSNPAIISEFSDKSPAREAGLLQGDRIVAI
                     DGKTINTYAEVGDAVRIYPGRTVVVTAERDGRQFEKPITLAERVITDQFGNETRLGIL
                     GIKNSTIEMRPVGAVEAVGYGFSESFAIMKMMAVGLQQIITGERSVKEMGGPVKIAKY
                     SGEMMTLGWLAFVEFVALVSINLAFINLLPIPTLDGGHLAMYAAEAVRRKPLGARSQE
                     WAFRGGMALVLAVAVFVTFNDLVSLFAK"
     gene            2902..>3399
                     /locus_tag="VFV30_11140"
     CDS             2902..>3399
                     /locus_tag="VFV30_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX4848690.1"
                     /translation="MNKPSLDRQPTLRTGLSMAAMLLGSTVLAGLPQAALAQTAPVQP
                     GPAAAPAPAGDTIQSITILGSQRLEPDTIRSYIKLRIGQPYTQAGADQAIKDLFATEL
                     FSEVQVRNNGGNVTIEVKENPVINRIILEGNKRIKSDKILPEIKLAPRQIFTRSKVRA
                     DVARII"
BASE COUNT          534 a         1132 c         1174 g          559 t
ORIGIN      
        1 gtggtcctgc tgggcgtgat cggcaccgat gtcggcgcct atttcaccgg ccgcacgctg
       61 ggcgggccca agattgcccc cgcgatcagc ccgtcgaaga cctggtccgg cctgatcggc
      121 gggatgctgg gcgcggcgct ggggatttac ctcgcgatgc ggatcgcttt caccgcgccg
      181 ttcatcgacg cgatgaacgc ggccatgtct ggcgagcccg acgccgtgac cctcgtgccc
      241 gggggcgaag ggccgtcgct ggtcggcgta ctggtggccg gcgcggtgct ggcggtggtc
      301 gcccaggccg gagacttctt tgaaagctgg atgaagcgcc gcgcgggcgt caaggattcc
      361 ggccggctga tcccgggcca cggcggcctg tttgaccgca ccgacggctt tctggccgtg
      421 ctgttcgtcc tcggcctgct ttcgctgcat tcgttcgtgt tcccggcatg accacccgca
      481 ccataaccat tctgggcgct accggctcga tcggggcctc gaccctcgat ctggtccggc
      541 gtgaccgcga tgcctggcgg gtggtggcgc tgactgccaa cggcaacgcc gccgagttgg
      601 cccggctggc gcgggaattc ggcgcggagc tggccgtggt ggccgacgag gccgcgcttc
      661 cggcgctgcg cgaggcgctg gcgggcagcg ggatcgaagc cgcgggcggg gcggcggcgc
      721 tggtcgaagc ggcggcccgg ccggccgaca tgaccatggc cgcgatcgtc ggctgtgccg
      781 gcctggcgcc gaccatggcg gcgattgccc gcggacagag cgtggccctg gccaacaagg
      841 aagcgctggt ttccgccggc gacgtgctga tggccgcggt ggcgcagagc ggggcgaccc
      901 tgctgccgac cgacagcgaa cataacgcga ttttccagtg cctggccgga aacgatctgt
      961 cccaggtccg ctcgatcacg ctgaccgcca gcggcgggcc gttccgcgat tgggcggcgg
     1021 ccgatctggc cgcggcgacg ccggaacagg cggtcaagca ccccaactgg tcgatgggcg
     1081 cgaagatcag cgtcgacagc gccacgatgt tcaacaaggg cctggaactg atcgaggcgc
     1141 accacctgtt cccggtcggg ctcgaccggc tgaagatcat cgtccacccg caatcggtgg
     1201 tccattcgat ggtcgaatac cgcgacggat cgaccctggc gcagctgggc ccgtcggaca
     1261 tgcgggtgcc gatcgcgtcc tgcctggcct ggcccgagcg gatggacacc ccgctgcccc
     1321 cgctcgatct ggcggcgatt ggcgagctga ccttccgcgc ccccgacgaa gaccggttcc
     1381 ccgcgacccg gctgtgccgc caggcggcgg agcagggcgg ggcggccccg gccgtgctca
     1441 acgccggtaa cgaagtggcc gtcgccgcat tcctttcggg acacgttgca ttcacccgga
     1501 ttgcggcaat ggtggaagac ctgatggggc gctatgctcc tgccgcaccg gtctcgctgg
     1561 gcgatgtcat cgccatcgat gccgaagcgc gggaccacgc gcgccgcttg ctggagcttg
     1621 cctgacttga tcgaatcgcc atcgctgttg ctgactgtcg gagccttcct gctgctgctc
     1681 gggccgctga tcgtgctgca cgaactgggc cactactggg ccgggcgcgc ctttggcgtg
     1741 cgggccgagg tgttttcggt cgggatcggc aaggagctgt ggcaccggat tgacaagcgc
     1801 ggcacccgct ggcgcttcgc cgcgattccg ctgggcgggt acgtcctgtt cgcgggcgat
     1861 gccgatgcca gcagccgtcc cgatccggcg ctggacgggg cctctgcgga gcggttgaag
     1921 gggaccttcc ccggcgcccc gctgtggcag cggaccctga tcgtgctggc cgggccggcc
     1981 gccaacctgc tggtcgcggt cgcgatcatc gcggccttcg tggcgatgat cggcgtgccc
     2041 tccaacccgg ccatcatctc ggaattcagc gataaatcgc cggcccgcga ggccgggctg
     2101 ctgcagggtg accggatcgt cgcgatcgac ggcaagacca tcaacaccta tgccgaagtg
     2161 ggcgatgcgg tgcggatcta tccgggccgc acagtggtgg tgacggccga gcgcgacggg
     2221 cgccagttcg aaaagccgat caccctggcc gaacgcgtca tcaccgacca gttcggcaac
     2281 gagacccggc tggggatcct ggggatcaag aacagcacca tcgaaatgcg cccggtcggc
     2341 gcggtcgagg cggtcggcta tggctttagc gagagcttcg cgatcatgaa gatgatggcg
     2401 gttggcctgc agcagatcat caccggcgaa cgctcggtca aggagatggg cgggccggtc
     2461 aagatcgcca agtactcggg cgaaatgatg acgctcggct ggctggcctt cgtcgaattc
     2521 gtcgcgctgg tctcaattaa tttggcattc atcaacctgc tgccaatccc gaccttagac
     2581 ggtgggcacc tggcaatgta tgccgcggaa gcagtccgcc gcaaaccgct gggtgcgcgc
     2641 agtcaggagt gggcatttcg cggcggcatg gcactggtgc tggccgttgc ggtgttcgtt
     2701 accttcaacg acctggtttc gctcttcgcc aaatgaggcg ggacgtgccg gaaagcggga
     2761 aaactgcgga aggcgtcggc aacttagctt gatccaaccg ggcgcatggg gcagagggcg
     2821 gcgacgtgtc tgcatgctgc gcacgggtgc cggtcagccg cgcctttccg tagccacaat
     2881 tgaacgggat atgccgtgct gatgaataaa ccgagcctgg accgccaacc gaccctgcgc
     2941 accggcctat cgatggctgc gatgctgctg ggcagcacgg tcctggcagg cctgccccag
     3001 gccgcccttg cgcagaccgc tcccgtccag cccggcccgg ccgccgcacc ggccccggcc
     3061 ggggacacga tccagtcgat caccatcctc ggctcgcagc ggctcgaacc cgatacgatc
     3121 cgctcctaca tcaagctgcg gatcggccag ccctataccc aggccggcgc cgaccaggcg
     3181 atcaaggacc tgttcgccac cgaactgttc tccgaagtcc aggtccgcaa caacggcggc
     3241 aacgtgacga tcgaggtcaa ggaaaacccg gtcatcaacc ggatcatcct ggaaggcaac
     3301 aagcggatca agtcggacaa gatcctgcct gaaatcaagc tggccccgcg ccagatcttc
     3361 acccggtcca aggtccgcgc cgacgtcgcc cggatcatc
//
LOCUS       DASUDV010000515         7179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_17881,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000515 DASUDV010000000
VERSION     DASUDV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17881"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="mnmA"
                     /locus_tag="VFV30_11145"
     CDS             <1..962
                     /gene="mnmA"
                     /locus_tag="VFV30_11145"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX4848691.1"
                     /translation="ASGRKGACCAGDDIRDARSVADRLGIAHYVIDHASEFREEVVER
                     FADDYLAGRTPIPCIRCNMGPKFTDLFAMARELGADCLATGHYVRRVIGPAGPELHRA
                     LDPARDQSYFLYGTTEAQLAYLRFPLGGLPKPEVRAIAEAEGLRVAAKPDSQDICFVP
                     DGDYAKVVRAVRPEGGEPGAIVHAESGEVLGEHRGIIHYTVGQRRGLEIGGQAEPLYV
                     TGLDAPGRRVLVGPKRLLAVSAARIVETNRIGPLPAGPLTAKVRSLAKPVPVTLEGPF
                     GEGTECTIRFANPEYGVAPGQAAVIYAGDRVVGGGWIESTEPA"
     gene            complement(991..1785)
                     /locus_tag="VFV30_11150"
     CDS             complement(991..1785)
                     /locus_tag="VFV30_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX4848692.1"
                     /translation="MSALLPTLMVERPWGRRSLPAPFVAPEGETIGEVWFDPPPALDA
                     LLVKYLFTSEALSVQVHPSDAQAPAGQRGKEECWLVLSAEPGARLGIGFDRELTADAM
                     RAAACDGSIEHLLTWHPVQAGDFFYIPAGTVHAIGAGIALIEIQQNSAITYRLYDYGR
                     PRELHLDAGMAVARGEPYPDRLRRQVPENGAVTLVEGPHFRLDRLDGPPDPATAARYA
                     GPLLVIPLDGSARIAGEKVESGACGLAAGIDDVTFAGRCLIAQPCA"
     gene            complement(1782..2825)
                     /locus_tag="VFV30_11155"
     CDS             complement(1782..2825)
                     /locus_tag="VFV30_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX4848693.1"
                     /translation="MTRPAIHPVVLCGGSGTRLWPRSRATKPKPFLPLVGTGTLFEAT
                     LGRCPPAAGFAAPIVVTGLAHLDHVEAQWPAGVGGEVIVEPSARNTAAAIALAALRLP
                     DDAVMLVCPSDHHIADTAAFQAAAAAAAELAGEGWLVSFGIEAKAPETGFGYLHRGEA
                     IGTLGFRVARFVEKPDLERAKQFLADGGYAWNGGIFAFRVGTFLAELERHRPAIAAQA
                     RLAVAGGRAEGTRFHPDPVAFAAIDSESVDYAVMENTDRAAMVPADMGWSDIGNWDAL
                     LTARPRDAEGNSVTGPAELVGCRNVLVETDGPTVSAIGLEDVVIVVDGNDVLVTTRAG
                     AQLVGKLSGAVNQ"
     gene            complement(2865..3212)
                     /locus_tag="VFV30_11160"
     CDS             complement(2865..3212)
                     /locus_tag="VFV30_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848694.1"
                     /translation="MATAKQNPTTRSPWRRRAVLLGLVLIGVLLATFWTPLMGYAEAG
                     SAYGARVACSCRYVGGRSLGDCKKDFEPGMELITLSENAAEKSVTATFPLITSHTATY
                     REGPGCQLETWPG"
     gene            3245..4369
                     /locus_tag="VFV30_11165"
     CDS             3245..4369
                     /locus_tag="VFV30_11165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4848695.1"
                     /translation="MPARRLSLILPLLALPALIGCGGETAPPPPAAPSAEALKAVVDK
                     PGVSREKLAGAVDALFSAEDTAETRAVLVLKDGRIVAERYGPGYHENTRFVSWSMAKT
                     VTAVMIGQLVADGRLRLDEPVPIPAWQRPGDPRGEITLRQLLQMRSGLRHTEAGDPPY
                     ESDEVRMLFLDGRDNMAAYAEAQPLEAVPGAKFEYSSATTVILADLAARALTTSTDPA
                     ERIRVVREYLRTRLFEPIGMKSMVPEFDATGTLIGGSLIHGTARDWGRFGEFLRNRGA
                     VKGAQLVPLAWIDFMIAPSPRNRGYGAQTWLNRPQPDGEVQWAGAPETVFSMNGHLGQ
                     YVVINPANGVVVVRLGKTQDGVHAPVRAAITRILTLFPKG"
     gene            4374..5438
                     /gene="gmd"
                     /locus_tag="VFV30_11170"
     CDS             4374..5438
                     /gene="gmd"
                     /locus_tag="VFV30_11170"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX4848696.1"
                     /translation="MREKTALITGITGQDGAYLARLLLEKGYRVHGIKRRSSSFNTSR
                     IDEIYEDPHVPDPKLTLHYGDLTDSTNLIRVVQETRPDEIYNLAAQSHVAVSFETPEY
                     TANSDALGTLRLLEAIRILGLEKTTRFYQASTSELYGLVQEVPQRETTPFYPRSPYAA
                     AKLYAYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAAAAIALGRQDVLY
                     LGNLDAQRDWGHAREYAEGMWLMLQQDEGDDYVLATGVTTTVRQFVEWAFADAGIELD
                     WRGSGVDEQGFCRKTGALRVAIDPRYFRPTEVDLLIGDPSKAHQKLGWRHKTGVRELA
                     REMVEADLIAMRTERIGRGG"
     gene            5438..6376
                     /locus_tag="VFV30_11175"
     CDS             5438..6376
                     /locus_tag="VFV30_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX4848697.1"
                     /translation="MTALAGKRVWVAGHTGMVGGALVRRLAGEGCEVLTAGRDELDLT
                     DQRAVLGWMHNHRPQLVFLAAARVGGIHANATRPVEFLRDNLLIETAVFTGAESAGVE
                     KLLFLGSSCIYPKHAEQPIREEALLTGALEPTNEWYAVAKIAGIKLAQAYRQQYGCDY
                     ISAMPTNLYGPGDNYDPEGSHVLPALIRKVHEAKAAGSPVTVWGSGSPLREFLYVDDC
                     ADACIHLMKTYSDGLHINVGSGEEISILDLTRLVMEVLGHDGEIVMDPSKPDGTPRKL
                     MDNSRLRAMGWAPQVSLRDGIARAYAAFLAGDGRNI"
     gene            complement(6373..>7179)
                     /locus_tag="VFV30_11180"
     CDS             complement(6373..>7179)
                     /locus_tag="VFV30_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4848698.1"
                     /translation="AMLGSLADTALDLVASLATLIGVWVAAQPADEKHRFGHGKAEAL
                     AAMIQVVLISISAIGIAFRAVDQWIAGSRPEAAEGGIAVSAIAMIATLALLAWQRHVI
                     RQTKSLAISTDHVHYQSDLLLNLAVIAALALDQYAGIAGADPVFGLAIALWLGWGAWG
                     ASQEAVNQLMDHEWPEERRAAFLEAVARHPEIKGLHDLRTRTSGNRDFVQFHLAVDPE
                     MTIRQAHVVMDEVEAILHQEFPGLEILIHPDPVGHVDPKDLSSKELLNRG"
BASE COUNT         1177 a         2500 c         2442 g         1060 t
ORIGIN      
        1 ccgccagcgg gcgcaagggc gcctgctgcg ccggcgacga catccgcgat gcccgcagcg
       61 tggccgaccg gctgggcatc gcccactatg tcatcgacca cgccagcgag ttccgcgaag
      121 aggtggttga acgctttgcc gacgattacc tggccgggcg caccccgatt ccctgcatcc
      181 gctgcaacat ggggcccaag ttcaccgacc tgttcgccat ggcccgcgaa ctgggcgccg
      241 attgcctggc gaccgggcat tatgtccgcc gcgtgatcgg gccggccgga ccggaactgc
      301 accgcgcgct cgatcccgcg cgcgaccagt cctactttct ttacggcacc accgaggcgc
      361 agctcgctta cctgcgcttt ccgctgggcg gcctgcccaa gcccgaagtg cgcgcgattg
      421 ccgaggccga gggcctgcgc gtcgcggcca agcccgacag ccaggacatc tgcttcgtcc
      481 ccgatggcga ctatgccaag gtggtccgtg cggtccggcc cgaagggggc gaaccgggcg
      541 cgatcgtcca cgccgaaagc ggcgaagtgc tgggcgagca ccgcggcatc atccattaca
      601 ccgtcggcca gcgccgcggc ctggagatcg gcggccaggc cgaaccgctc tacgtgaccg
      661 ggctcgacgc gccggggcgg cgggtgctgg tcgggcccaa gcgcctgctg gccgtctccg
      721 cggcgcgaat cgtcgaaacc aaccggatcg gccccctgcc cgccgggccg ctgaccgcca
      781 aggtccgctc gctggccaag cctgtgccgg tcacgctgga aggccccttt ggcgaaggca
      841 cggagtgcac gatccgcttc gccaatccgg aatacggcgt ggcaccgggt caggcggcag
      901 tgatctatgc gggagaccgg gtggtcggcg gcggctggat cgaatcgacc gagccggcct
      961 gacccgcaag gccctaggcg caggaaggcc ctaggcgcag ggctgcgcga tcaggcagcg
     1021 ccccgcaaat gtgacatcgt cgataccggc cgcgagaccg caggccccgc tttccacctt
     1081 ttcgcccgcg atccgggccg aaccgtcgag cggaatcacc agcagcgggc cggcatagcg
     1141 cgctgccgtg gccggatcag gcgggccatc gagccggtcg agccggaaat gcggcccctc
     1201 gaccagcgtc accgcgccat tctcgggcac ctggcggcgc agcctgtccg gatagggctc
     1261 gccccgggcc acggccatgc cggcatcgag gtgcagctcg cgcgggcggc cgtagtcata
     1321 gagccggtag gtgatcgcgc tgttctgctg gatctcgatc agggcaatgc ccgccccgat
     1381 cgcgtgaacc gttccggcgg ggatgtaaaa gaagtcccca gcctgcacgg ggtgccaggt
     1441 cagcaggtgt tcgatgctgc cgtcgcacgc ggcggcgcgc atcgcatcgg cggtcagttc
     1501 gcggtcgaag ccgatgccca gccgcgcgcc gggctcggcc gaaagcacca gccagcattc
     1561 ctccttgccg cgctgcccgg ccggggcctg ggcgtcggac gggtggacct gaaccgacag
     1621 cgcctcgctg gtgaacaggt atttgaccag cagcgcatcg agcgcgggcg gcggatcgaa
     1681 ccagacctcg ccgatcgttt ccccctcggg cgccacgaag ggcgcgggca ggctgcggcg
     1741 gccccacggc cgctcgacca tgagggtggg cagcagggcg ctcactggtt gaccgcgccg
     1801 ctcagcttgc cgaccagctg cgcaccggcc cgggtggtga ccagcacgtc gttcccgtcg
     1861 accacgatca ccacgtcttc cagcccgatc gccgaaaccg tgggcccgtc ggtttcaacg
     1921 aggacgttgc ggcaaccgac cagttcggcc ggcccggtca cgctgttgcc ttccgcatcg
     1981 cgcgggcggg cggtcagcag ggcatcccag ttgccgatat cggaccagcc catgtcggcc
     2041 ggaaccatcg ccgcgcggtc ggtgttctcc atcaccgcat agtcgaccga ttcgctgtcg
     2101 atcgcggcga aggcgacggg atcggggtgg aaccgcgtgc cctccgcgcg ccctccggcc
     2161 acggccaggc gggcctgggc ggcgatcgcc gggcggtgcc gttccagctc ggccaggaaa
     2221 gtgccgaccc ggaaggcaaa gatcccgccg ttccaggcat agccgccgtc ggccagaaac
     2281 tgcttcgccc gctcaagatc cggcttctcg acaaagcgcg cgacccggaa gccgagcgtg
     2341 ccgatcgcct cgccgcggtg aagatagccg aacccggttt cgggggcctt ggcctcgatc
     2401 ccgaacgaga ccagccagcc ctccccggcc agctccgccg cggcggcggc ggccgcttgg
     2461 aacgcggcgg tatcggcaat gtggtgatcg ctcgggcaaa ccagcatgac cgcatcgtcc
     2521 ggcaggcgca gcgcggccag cgcgatcgcc gcggcggtgt tgcgggccga tggctcgacg
     2581 atcacttccc cgccgacccc ggccggccac tgcgcctcga catggtcgag atgcgccagg
     2641 cccgtgacaa cgatcggcgc ggcgaacccg gcggcgggcg ggcagcggcc cagcgttgcc
     2701 tcgaacaggg tgccggtacc gaccagcggc aggaacggct tgggcttggt ggcgcggctg
     2761 cgcggccaca ggcgggtgcc gctgccgccg cacagcacga caggatggat tgcgggtcga
     2821 gtcattccgg agtgcctagc acaagttgat cgccaagggg ttactcagcc cggccaggtt
     2881 tcaagctggc agccggggcc ttcgcggtag gttgcggtgt ggctggtgat cagagggaac
     2941 gtggccgtga cgcttttctc ggccgcgttc tcggacaggg tgatcagttc catgccgggt
     3001 tcgaaatcct tcttgcaatc ccccaggctg cgcccgccga catagcggca ggaacaggcc
     3061 acccgcgcgc cataggccga tccggcctcg gcataaccca tcagcggggt ccagaaggtg
     3121 gccagcagca ccccgatcag aaccagcccc agcagcaccg cgcggcggcg ccacggggat
     3181 cgggtggtgg ggttctgctt agcggttgcc attgtcgagc ctttgggcaa gggatgcggg
     3241 caccatgcct gcgcgccgcc tgtcccttat ccttccgctg cttgctctgc cagccctgat
     3301 cggctgcggc ggcgagacgg cgccgccccc gccggccgcg cccagcgccg aggcgctgaa
     3361 ggcggtggtc gacaagcccg gggtcagccg cgaaaagctg gctggcgcgg ttgacgcact
     3421 gttcagcgcc gaggacaccg ccgaaacccg cgccgtgctg gtgctgaagg acggccggat
     3481 cgtggccgag cgctatggcc ccggctatca cgagaacacc cggttcgtgt cgtggtcgat
     3541 ggccaagacg gtgaccgcgg tgatgatcgg ccagctggtc gccgatggcc ggctgcggct
     3601 ggacgagccg gttccgatcc cggcctggca gcgccccggc gatccgcgcg gggaaatcac
     3661 cctgcgccag ctgctgcaga tgcgctcggg cctgcgccac accgaagccg gcgatccgcc
     3721 ctatgaaagc gacgaggtgc ggatgctgtt cctcgacggg cgtgacaaca tggccgccta
     3781 tgccgaggcc cagccgctgg aagcggtgcc cggcgccaag ttcgagtatt ccagcgccac
     3841 cacggtgatc ctggccgacc tggccgcccg cgcgctgacc accagcaccg atccggccga
     3901 acggatccgg gtggtgcgcg aatacctgcg cacccgcctg ttcgaaccga tcggcatgaa
     3961 aagcatggtg cccgaattcg acgccaccgg caccctgatc ggcggcagcc tgatccacgg
     4021 cacggcgcgc gactggggcc ggtttggcga gttcctgcgc aaccgcggcg ctgtgaaggg
     4081 cgcgcagctg gtgccgctgg cctggatcga tttcatgatc gcgcccagcc cccgcaaccg
     4141 cggctatggc gcacagacct ggctcaaccg cccgcagccc gatggcgagg tgcaatgggc
     4201 cggcgcgccg gaaacggtct tttcgatgaa cggccacctc ggccagtacg tggtgatcaa
     4261 tccggcgaac ggggtggtgg tggtccgcct gggcaagacc caggacggcg tccacgcgcc
     4321 ggttcgcgcc gcgatcaccc ggatcctcac tctcttcccc aaaggatagc cccttgcgcg
     4381 agaaaaccgc tctcatcacc gggatcacgg gccaggacgg cgcctatctg gcgcggctgc
     4441 tgctggaaaa gggctaccgc gtccacggca tcaagcgccg ctcgtcctcg ttcaacacca
     4501 gccggatcga cgagatctac gaggatccgc acgtgccgga tcccaagctg acgctgcact
     4561 atggcgacct gaccgattcg accaacctga tccgggtggt ccaggaaacc cgcccggacg
     4621 agatctacaa cctggccgcg caaagccacg ttgcggtcag ctttgaaacg ccggaataca
     4681 ccgccaattc cgacgcgctg ggaaccctgc gcctgctgga ggcgatccgc attctgggtc
     4741 tcgaaaagac cacccggttc tatcaggctt ccacttccga gctctacgga ctggtgcagg
     4801 aagtgccgca gcgcgagacc accccgttct acccccgcag cccctatgcc gcggccaagc
     4861 tctatgccta ctggatcacg gtcaactacc gcgaagcgta cggcatccac gcctcgaacg
     4921 ggatcctgtt caaccacgaa agcccgctgc ggggcgaaac ctttgtcacc cgcaagatca
     4981 cccgcgccgc ggcggcgatc gcgctgggcc ggcaggacgt gctctacctc ggcaatctcg
     5041 acgcacagcg cgactggggc cacgctcgcg aatacgccga gggcatgtgg ctgatgctgc
     5101 agcaggacga aggcgacgac tatgtcctgg cgaccggggt caccaccacg gtccgccagt
     5161 tcgtcgaatg ggccttcgcc gatgccggga tcgaactcga ctggcgcgga tcgggcgtcg
     5221 acgaacaggg cttttgccgc aagaccggcg cgctgcgggt cgcgatcgat ccacgctatt
     5281 tccgcccgac cgaggtggac ctgctgatcg gcgatcccag caaggcgcac cagaagctgg
     5341 gctggcggca caagaccggg gtgcgcgaac tggcccgcga aatggtcgaa gccgatctca
     5401 tcgcgatgcg gaccgagcgg atcgggcggg gcggctgatg accgcgctgg ccggaaaacg
     5461 ggtctgggtt gcaggccaca ccggaatggt cggcggggcg ctggttcgcc gcctggccgg
     5521 cgagggctgc gaagtgctga cggcggggcg cgacgagctc gacctgaccg accagcgcgc
     5581 ggtgctgggc tggatgcaca accaccgccc gcagttggtc tttctggccg cggcgcgggt
     5641 cggcgggatc catgccaatg ctacccgtcc ggtcgaattc ctgcgcgaca acttgctgat
     5701 cgagaccgcg gtgttcaccg gcgccgaatc ggccggggtg gaaaagctgc tgttcctggg
     5761 ctcctcgtgc atctacccca agcacgccga gcagccgatc cgcgaagagg cgctgctgac
     5821 cggggcgctg gagccgacca acgaatggta tgcggtcgcc aagatcgccg ggatcaagct
     5881 ggcccaggcc taccgccagc aatacggctg cgattacatc agcgcgatgc cgaccaacct
     5941 ttacggcccc ggcgacaatt acgatccgga aggcagccat gtcctgcccg cgctgatccg
     6001 caaggtccac gaagccaagg ccgccggcag cccggtgacg gtctggggat cgggcagccc
     6061 cttgcgcgaa ttcctttatg tcgacgactg cgccgacgcc tgcatccacc tgatgaagac
     6121 ctattcggac ggcctgcaca tcaatgtcgg ctcgggcgag gagatctcga tcctcgatct
     6181 gacccggctg gtcatggagg tgctggggca tgacggagag atcgtcatgg acccgtccaa
     6241 gcccgacggc accccgcgca agctgatgga caacagccgc ctgcgggcaa tgggctgggc
     6301 cccgcaggtc tctttgcgcg acgggatcgc aagggcctat gccgcgtttc tcgccggcga
     6361 cgggcggaat atctaacccc ggttcagcag ctctttcgag gacaggtcct tcggatcgac
     6421 gtgcccgacc ggatcggggt ggatcaggat ttccagcccc gggaattcct ggtgcaggat
     6481 cgcttccacc tcgtccatca ccacgtgggc ctggcggatg gtcatctccg gatcgaccgc
     6541 gaggtggaac tgcacgaaat cgcggttgcc cgacgtgcgg gtgcgcagat cgtgcaggcc
     6601 cttgatctcg ggatggcggg ccacggcctc cagaaaggcc gcgcggcgct cttcgggcca
     6661 ttcgtggtcc atcagctggt tgaccgcttc ctgcgaagcg ccccaggcgc cccaccccag
     6721 ccacagcgcg atggccaggc caaagaccgg atcggccccg gcgatcccgg cgtactggtc
     6781 cagcgccagc gcggcgatca ccgccaggtt gagcagcagg tccgactggt agtggacgtg
     6841 atcggtgctg atcgccaggc tcttggtctg gcggatcacg tggcgctgcc aggccagcag
     6901 ggccagggtg gcgatcatgg cgatggccga gaccgcaatc ccgccctcgg ccgcttcggg
     6961 ccgcgacccg gcgatccact gatcgaccgc gcgaaaggca atgccgatcg ccgagatcga
     7021 gatcagcacg acctggatca tcgcggccag cgcttcggcc ttgccgtgcc cgaagcggtg
     7081 cttctcgtcg gccggctgcg ccgcgaccca gacgccgatc agcgtggcca ggctggccac
     7141 cagatcgagc gcggtatcgg ccaggctgcc gagcatggc
//
LOCUS       DASUDV010000516         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_39939,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000516 DASUDV010000000
VERSION     DASUDV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39939"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFV30_11185"
     CDS             <1..436
                     /locus_tag="VFV30_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4848699.1"
                     /translation="GTYNPLLAKDDENRVRLVEDRVRYWIGVGAQPTDRVARLLDKAG
                     IKDRAATVNPKKGEPGQKAKDRAEEKAEKAAAAAEAAKEAEEAAAAAAAAPVVEEAPA
                     AEEAPVAEEAAAEEAAAEAPAAEEAAAEEAPAAEAGEEAAEG"
     gene            440..931
                     /gene="rimM"
                     /locus_tag="VFV30_11190"
     CDS             440..931
                     /gene="rimM"
                     /locus_tag="VFV30_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070962.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4848700.1"
                     /translation="MDADRPVTLAAIVGAHGVAGEVRLKLFGEGVEQLRRFRAFNASA
                     LSLEKLRDDGKGGAIARFAEVKDRTAAERLRGTALTVPRSALPPLDEGEYYHADLIGL
                     PALSDLGEALGICIAVENFGAGDVLEIERPDGKKFMVPMRAQAVPEWNAERLVINAAF
                     AVD"
     gene            complement(906..1769)
                     /locus_tag="VFV30_11195"
     CDS             complement(906..1769)
                     /locus_tag="VFV30_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848701.1"
                     /translation="MRWLIIASALAPLASPASAEPVGLCRFDVASLTFAGTPLDQARC
                     LLRKVRLFGETEEQPLPKVLTGLLATDSAPSSDQTGAAIAAFPEPYRSYAIEHRARPA
                     SQTAAGLPLLYFVIHDTSTPFYGKERFPRNLDRDPKINDFTPYMDGTFAAEPVAHIFL
                     SRFGQIWAGHDFSEGWRATKLEVQIAGVPARGRFTHIEVVQPRRFVPGSDSLQRTHGP
                     RPGFSNEQYRQLAALYVYASARAGRWLIPAFHATIDAGIPDGHDDPQNFELEKFGKAL
                     GRLINPPRRRR"
     gene            1845..2195
                     /locus_tag="VFV30_11200"
     CDS             1845..2195
                     /locus_tag="VFV30_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX4848702.1"
                     /translation="MRELRGNFDHSDATLPGNRLAQSAKPLKARVFKSGNSLAVRMPK
                     ELGLEAGVELQLEVLSDGAIVLRKPAPVRQKIDISGFAGKLPGFKPAPRQDFEERPST
                     IAAREAARKAALGK"
     gene            2192..2572
                     /locus_tag="VFV30_11205"
     CDS             2192..2572
                     /locus_tag="VFV30_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX4848703.1"
                     /translation="MNRYLIDSALAIYSMGGAVDSPLNQRLADCEPGMVSISAISFAE
                     VALGTWNGRPPRQDVLDAFIRVIPLVEFDEEAARAYAKLPFRRARFDRLLAAHALSIG
                     AIVITNNEADFADVPGLVVENWMS"
     gene            2569..3315
                     /gene="trmD"
                     /locus_tag="VFV30_11210"
     CDS             2569..3315
                     /gene="trmD"
                     /locus_tag="VFV30_11210"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4848704.1"
                     /translation="MTFAATVLTLYPEMFPGPLGISLAGRAREEGKWSLDTVQIRDFA
                     IDKHKTVDDTPAGGGAGMVLRVDVLAAAIDHARASHPDCPVIAMTPRGKPLSQARVRE
                     LAAGPGVTLLCGRFEGFDERIFEGRGVEEVSVGDIVLSGGEPAAIMLLDACIRLLPGV
                     MGAASSGTEESFEQGLLEYPHYTRPQEWEGRTIPEVLRSGDHAKIAAWRKSQSEIDTR
                     LRRPDLWELHEEARVLSASGARRKKKEMDQ"
     gene            3312..>3420
                     /locus_tag="VFV30_11215"
     CDS             3312..>3420
                     /locus_tag="VFV30_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX4848705.1"
                     /translation="MNLIQQIEAEEIAKAAKDIPEFRPGDTVRVGVKVIE"
BASE COUNT          647 a         1042 c         1174 g          557 t
ORIGIN      
        1 cggcacctac aacccgctgc tcgccaagga cgatgaaaat cgcgtccgtc tggtcgaaga
       61 ccgcgtgcgt tactggatcg gcgttggcgc ccagccgacc gatcgcgttg cccgcctgct
      121 cgacaaggcc gggatcaagg accgcgccgc gaccgtgaac ccgaagaagg gtgaacctgg
      181 ccagaaggcc aaggaccgcg ccgaggagaa ggctgaaaag gccgccgctg ctgctgaagc
      241 cgccaaggaa gccgaagaag ccgccgccgc tgccgccgct gccccggtgg tggaagaagc
      301 tccggccgcc gaggaagccc cggtcgctga agaagccgct gctgaagaag ctgccgccga
      361 agccccggcc gctgaagaag ccgccgccga agaagctccg gctgccgaag ccggcgaaga
      421 agcggccgaa ggctaagcct tggacgccga ccggcccgtt acccttgccg ccattgtcgg
      481 cgcgcacggg gtggcgggcg aggttcggct caagctgttt ggtgaaggcg tggaacagct
      541 gcggcggttc cgcgccttca acgcatcggc gctcagcctc gaaaagctgc gcgacgatgg
      601 caagggcggc gcgatcgccc gctttgccga agtgaaggac cggaccgcgg ccgaacggct
      661 gcgcggcacc gcgctcaccg tcccgcgctc ggccctgccg ccgctggacg aaggcgaata
      721 ctatcacgcc gacctgatcg gattgcctgc cctgtccgat cttggcgagg cgctgggcat
      781 ctgcattgcc gtcgaaaact tcggcgccgg cgacgtgctc gagatcgagc gtccggacgg
      841 caagaaattc atggtgccga tgcgcgccca ggccgtgcct gaatggaacg ccgagcgcct
      901 ggtgatcaac gccgccttcg cggtggattg atcagccgcc ccagcgcctt gccgaacttc
      961 tccagctcga aattctgcgg atcgtcgtgc ccgtcgggga tgccggcatc gatcgtggcg
     1021 tggaacgccg ggatcagcca gcgcccggcg cgggccgagg catagacata aagcgcggct
     1081 agctggcgat actgttcgtt ggaaaagccc ggccttgggc catgggtgcg ctgcaggctg
     1141 tcgctgcccg gaacgaagcg gcgtggctgc accacctcga tatgggtaaa acgcccgcgc
     1201 gccggcacgc ccgcgatctg cacctccagc ttggtggcgc gccagccttc ggaaaaatcg
     1261 tgcccagccc agatctgccc gaagcgcgag aggaagatgt gcgccaccgg ttctgccgca
     1321 aaggtgccgt ccatataggg cgtgaaatcg ttgatcttcg gatcgcgatc gagatttcgg
     1381 gggaagcgct ccttgccgta gaacggcgtg ctggtgtcgt ggatcacgaa atagagcagc
     1441 ggcaggcccg ctgcggtttg tgaggcgggt cgggcgcggt gttcgatcgc atagctgcgg
     1501 tagggctcgg ggaatgccgc aatggctgcc ccggtctggt cagaagaggg tgcgctgtcg
     1561 gtcgccagca ggccggtcag aaccttaggc agcggctgct cctcggtctc gccgaacagc
     1621 ctgaccttgc gcagcaggca gcgcgcctga tcgagcgggg ttcccgcgaa tgtcaggctg
     1681 gcgacatcga accggcacag cccgaccggc tcggcagatg cgggtgaggc tagtggagcc
     1741 aaagccgagg cgatgatcag ccagcgcaat tgacgcgcct agatccgatg tatatacaca
     1801 atgtatatac ggagcaatcc atgaccaagc ccgatcccaa tcttgtgcgc gaactgcgcg
     1861 ggaacttcga tcatagcgat gcaacattgc cgggcaatcg ccttgcccaa agcgcaaagc
     1921 cgttgaaggc aagggtgttc aaatcgggca attcgctggc ggtgcggatg ccaaaggaac
     1981 tcgggctgga ggcaggagtg gaactgcaac tggaagtgtt gtcggacggg gcgattgtgc
     2041 tgcgcaagcc cgcgccggtc cggcaaaaga tcgacatcag cggtttcgcg gggaaactgc
     2101 cgggcttcaa acccgcccca cgccaggatt ttgaagagcg tcccagcacg atcgcggccc
     2161 gtgaagcggc ccgaaaggcg gcgctgggca agtgaaccga tacctgatcg attccgctct
     2221 ggcgatctat tcgatggggg gagcggtgga ttcgccgctc aaccagcggc tggctgattg
     2281 cgaaccgggc atggtctcga tctcggcaat cagttttgcc gaagtcgcgc tgggcacctg
     2341 gaatggtagg ccgcccaggc aggatgtgct ggatgccttc attcgggtca ttccgcttgt
     2401 cgagttcgac gaggaagcgg cacgggccta tgccaagctg cccttcaggc gcgcacgttt
     2461 cgaccggctg ctcgcggccc acgcgctcag catcggggcg attgtgatta ccaacaatga
     2521 ggccgacttt gccgatgtgc cggggttggt agtagagaac tggatgtcat gaccttcgcc
     2581 gcgaccgtcc tcacccttta cccggaaatg ttcccggggc cgctgggcat cagcctggcc
     2641 ggccgcgcgc gcgaagaggg gaagtggtcg ctcgacaccg tccagatccg cgatttcgcg
     2701 atcgacaagc acaagacggt cgatgacacg ccggccggcg gcggggcggg aatggtgctc
     2761 agggtcgatg tgctggccgc cgcgatcgat catgcccgcg ccagccatcc cgactgtccg
     2821 gtgatcgcca tgaccccgcg cggaaaaccg ctcagccagg cccgggtgcg cgagcttgcg
     2881 gcaggccccg gcgtgaccct gctgtgcggg cggttcgagg gatttgacga gcggatcttc
     2941 gaagggcgcg gagtggaaga ggtatcggtt ggcgatatcg tcctgtcggg cggcgagccg
     3001 gcggcgatca tgctgcttga cgcttgcatt cggctgcttc ccggcgtaat gggcgccgct
     3061 tcgagcggta ccgaggagtc ctttgaacaa gggctgctcg aatatcctca ctatacccga
     3121 ccccaggaat gggaagggcg cacgatccct gaagtgctgc gatcggggga tcatgcgaag
     3181 attgcggcgt ggcgcaagtc acagtcggaa atcgacacac ggttacgcag gccggatctt
     3241 tgggagcttc atgaggaagc tcgggtcctg tctgcctctg gcgcgcggcg aaaaaagaag
     3301 gaaatggacc agtgaacctg atccagcaga tcgaggccga ggaaatcgcc aaggccgcca
     3361 aggatattcc ggagttccgt ccgggcgaca ccgtccgcgt cggcgtgaag gtcatcgaag
//
LOCUS       DASUDV010000517        20151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_591737,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000517 DASUDV010000000
VERSION     DASUDV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 20151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20151
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591737"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFV30_11220"
     CDS             <1..1291
                     /locus_tag="VFV30_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX4848706.1"
                     /translation="SGALDRPVQQIGLTTFRPPYTPQTFGALAGHAKDALFQPTRKTP
                     IDGWAEAHGAVYEPVALWRRARYFPKPGEDMLAAVNRECLAVRNAVGIFDASTLGKIE
                     VVGPDAAEFLNRMYTNPWKALEPGRCRYGLLLKEDGFITDDGVSARLAPDRFHLTTTT
                     GGAARVLNMMEDYLQTEWPDLNVWLTSVSEQWSVIALQGPKARDVIAPFVEGIDLSPE
                     AFPHMAVREGTICGVPTRLFRVSFTGELGFEINVPAAYGRAVWEQLWEAGQAHGITAY
                     GTETMHVLRAEKGFIIVGQDTDGTLTPYDAGLEWAVGKKKPDFVGKRSMARPDIVAPG
                     RKQLVGLLTDDPAEVLEEGAQIVADPNQPVPMDMLGHVTSSYWSATLGRSIALAMVAG
                     GQGRMGETLYIPMPGRTIAAKVTGMVFYDPEGARLNG"
     gene            1284..1793
                     /locus_tag="VFV30_11225"
     CDS             1284..1793
                     /locus_tag="VFV30_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX4848707.1"
                     /translation="MADTLARLEPVPTEPAAYPGVTVALAPPMRRWSLRARDPKLLEK
                     AIGRKVPRTIGETLDGIACLGPDEWLWRSAEGVPAGMAEGQPVSIVDVSERSVAFRIE
                     GPLARAALNAGCPRDLERFAVGQTRRTVYEGVEIILHRAGEEQWTAEVWRSFAEWFWL
                     SLNAAAAHP"
     gene            complement(1790..2659)
                     /locus_tag="VFV30_11230"
     CDS             complement(1790..2659)
                     /locus_tag="VFV30_11230"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX4848708.1"
                     /translation="MFATDLRVAASGFRLVTAASPRCARQMPGTGLLLHSLSQYSAGT
                     RAEVQALRAFPTAVAVADVVPGSPAAQADLRGGDGIAAINGEPLPASAPPGSGSTALR
                     DAAEDRLLALPPSAPIRLRVRRGEAWLETVLVPVPTCATRIEVVAGSALKARSDGRII
                     QIGQAFAESLDDEGLAAALAHELAHTVLDHRRQLAPLEQAAPGPALRRQRTALARQFE
                     DEADLASLDLLRLACWDPAIAPKFLRRTGVRFDPFLGGSGTHRSARERAARMERAIPA
                     LPALPDSCRVKPV"
     gene            2993..3217
                     /locus_tag="VFV30_11235"
     CDS             2993..3217
                     /locus_tag="VFV30_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848709.1"
                     /translation="MTKKLMAAAAAFAMTATPVLAAGETQAAPVRDAAPVAQSEQLFD
                     GDGGGWLLFVLLGAAAVVGLIVAMDDPASP"
     gene            complement(3373..4746)
                     /locus_tag="VFV30_11240"
     CDS             complement(3373..4746)
                     /locus_tag="VFV30_11240"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4848710.1"
                     /translation="MNLGITLRQAATLLYLLLILLLGGASAAGFGGNLVLQIAGAGLI
                     AWTLWSEEPGKGLRTGAGAFMIGLAVLALIQFLPLPPALWQMLPGREAVAQGFALTGS
                     ELPWLSYSLDPWSSLQSLVWWIPAGALFYAMRAESSPETRHAIWVILAVAYASVVLAA
                     MQVVSGSGYFYAITNRGNGVGLFSNSNHFGSFMLIATALAAGQWLHDRPTRRPIAPRI
                     SLNVELLLRLAPLVLGVLLSRSLAAGLLLLPLLGGIALVVYPQWRIRWPLIGIAALAF
                     AVGIVWLLASGAVSNDLMSKSGTAGISRGEFLANGSAIARDFAPLGAGIGTFRDLYPW
                     YESATNVSTTFVNHAHNDLLELVIEAGIPGLIVLALFLRWIGGKSWQLWNAPRTEHPV
                     ALAATLATALVLTHSLVDYPLRTAAVSCLIALCAIFACRPPALRGSAALAQSSPGAGK
                     RETMLQI"
     gene            complement(4743..5960)
                     /locus_tag="VFV30_11245"
     CDS             complement(4743..5960)
                     /locus_tag="VFV30_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848711.1"
                     /translation="MRSSIMPGGITFQSVRRKLLAGGVLAALTAAVLAANTARVAETP
                     AAGAGSQLSALGPGALGSLRFELSKLGTGKPELDKDLAQRALLSSPLSADPFIGFAFL
                     GLRDNPKGTIGNEDKMLLEALRRDPRSRTARVLRLRQLAASGELKPAFQELAILQRLN
                     PQLVDVVMGSIAQRIATPLNVDQALDAISGHDSLYLPLVASMGGKEKTPEVVVRLAER
                     LPARVLDDPDTRKNLIKHLVAVGAFSQARTIWSRGMAAGGGLVHSPDFSDARAPAPFN
                     WLLSVNTTGAAERSKDGGIYVSYYDRQPGALVSQLLTLAPGSYRATAEFEVISGGADN
                     VRLRVSCHGQTGYLAEIPLRQGSRSVALPFAVPAQGCNGQILALFGAASQKRGEMQVS
                     VRRIDVVPAGGQQ"
     gene            complement(5960..8152)
                     /locus_tag="VFV30_11250"
     CDS             complement(5960..8152)
                     /locus_tag="VFV30_11250"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX4848712.1"
                     /translation="MRQGHTIQGDDPSTGAGHPHAAEHGFDLSHLLSILLDRYKLILA
                     MGAAGLLLGIVAALMATPLYRASALIQYEPGAADLLEPGRYVAGQRSAKGNTQEGILT
                     QIGLLQSESLARQVAQDLNLVSNPAYGGAGGTIDQRTNNAAAAVQGSITAEPIKGSSL
                     IRISAVSTDPRTAAQIANAVVKGHISASLARRFESSSYARDFLSDQIARTKESLEVSE
                     RNLNNYAIKAGVFRNTTRAADGREVEGTSLSQSKLSSLNEALARAKVNRIAAEQRYKE
                     AELDGSLDQALSLSPLIQQKTELQAQYDEKLKIYKPDYPSMIELKARIDRIDAAINAE
                     RKRGRTNKRAELYGEYRAAVQTEAELQVAVNEAKGEVLADRNIAIEYNILQRETDTNR
                     SLYDALLQRYKEVGVAGGIGKSEISMVDEAKVPAGPFRPRPLVNAIVGLLAGLALGVG
                     LALGLQILFDSITDTRDVRTKLRLPVLGAIPVETDGRAPVEALADSKSQMSEAYHSVR
                     TALKFARPEGLPHSLLVTSTRPGEGKSTSSYAIALSVAKLGSKVLLIDADLRKPTFAS
                     GRKDGMGLGHLLVSDDPLGDYIEATKTPNLSLLPVGRISASAAELLSSNRLPLLVKEA
                     QGVFDMVVIDGPPVLGLADAPLLASVVDATALVVECRGARTAEVQEMVRRLADAGAHL
                     VGVILTKVTVKRSGYGYGYGYGYGHYSHSDDNDSDDTISARRIDVAKS"
     gene            complement(8201..8923)
                     /locus_tag="VFV30_11255"
     CDS             complement(8201..8923)
                     /locus_tag="VFV30_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX4848713.1"
                     /translation="MGSRSASQSGFQSAFRLIVLAVMALGLAACSARGGKIPYAPADF
                     GPPDKQAAEDIVYDLPLGPLDMVRVSVFRVPELSSDYQVDARGMVDMPLLGLINVRDV
                     TPTQFADELEKRYAAKYLNNPEITVRILSTNNNSVTVEGGVNAPGVYVLPGRTTLLGV
                     MAMARGINTNDGNPRRVAIFRKKDGKTLAAAFDLVSIRRGEMDDPDIYPGDRVVVDSS
                     QLRPAYRDVLMSIPILSLFLAL"
     gene            complement(9035..10183)
                     /locus_tag="VFV30_11260"
     CDS             complement(9035..10183)
                     /locus_tag="VFV30_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4848714.1"
                     /translation="MERRPDAPLVIVCANLAWNLVNFRSGLIKGLIEAGYRVLAAAPA
                     DPDMEARLRALGADFTAVPIDAMGLAPHRDIATFLAFRRLIAQHRPAAWLSWTIKPNV
                     YGSLAAGLAGVPAFPNVSGLGTAFIRRNVLTVIAQQLYRTGFRKAPTVFFQNRDDPAL
                     FLQNRLVRPEQIAILPGSGIDTAAWAPASSERPPPRTFLMLARVVADKGVREYVEAAR
                     RVRARWPDARFRLMGALGAANRTAIPEAEVRGWIAEGAIEYAEPVDDVRPEIAAADFV
                     VLPSYREGLSRVLLEAAAMARPIVTTDVTGCRDIVREGENGFLCAPRDAAALAAALEQ
                     AAATGDADWLRMGQAGRARVLAEFSQEMVTGQYLAALSRSGILPPVTA"
     gene            complement(10188..11249)
                     /locus_tag="VFV30_11265"
     CDS             complement(10188..11249)
                     /locus_tag="VFV30_11265"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HEX4848715.1"
                     /translation="MYWLLLAYPAVMALAYPARTEQTRQSAGQVLALAGFVLFYTLMA
                     GMRWDVGGDWGNYEEIYDDIALSSLSYAMGVTDPLYGLVNWVSAQFDLDIYFVNGVCA
                     FLLVLGVAQVVGRFRDPWLAVLMAVPYLLIVVGLGYIRQGAAIGLLLMAIAAFDRGKL
                     IRTGVFLLMAIGFHATAIMVIPLFGYSLGTRYRLFAVVVGLATIYAYFAIVVPALDRF
                     NAGYIDQELESTGALARLLMSFIPSLLLLLRWRHIGGSSRLRPIWISVAIANVLLLLL
                     LFVSPSSTAVDRAGLYFSIAQLLVFGEFRNLLPTPERMTAFVRVLLIGVASAVQVVWL
                     VYATNAEFWVPYKTLFEAI"
     gene            complement(11300..11899)
                     /locus_tag="VFV30_11270"
     CDS             complement(11300..11899)
                     /locus_tag="VFV30_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848716.1"
                     /translation="MRLVPRFFAGLRKAHWALRSAAFRTYLRLAYPGVRCHRSVIFGK
                     GVTVRAFDGARLEIGEGTVVLEHAWIQVEGGSLTIGRNCLIGRAAVVVCSEAIEIGDG
                     TLTAEHVTIRDQDHNYAGEGRLETQGSSTAAIRIGHDVWLAAKVTVTRGVEIAPHAVV
                     GANAVVTRSLPVRGVYAGAPARLVKPVEAAAVEPDAQRR"
     gene            complement(11896..12360)
                     /locus_tag="VFV30_11275"
     CDS             complement(11896..12360)
                     /locus_tag="VFV30_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX4848717.1"
                     /translation="MSFEMMPDTAAILRRHVADNGPLPVTVIERALSDRSGDRVTASV
                     EPGKHGQASIVAGDQDARRSVSVETVTLDEALADLGRIDLIKLDPEGAEFMALSGARE
                     VLLRTGCVVFESNDRDARIFALLHSAGFAVEQLDAFDFLARPLAGANPKAAQ"
     gene            complement(12345..12734)
                     /locus_tag="VFV30_11280"
     CDS             complement(12345..12734)
                     /locus_tag="VFV30_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848718.1"
                     /translation="MALNYPYRLRMWLNLLRELQPGDGRSRRVLWLSALAAPFTSLRA
                     LDSYRQPHLIGDIAVAVPGVGRFAVRAGTDDLLHVMTSREPHVRRVIEAELPPGGTFV
                     DGGASIDSSRWLPREGLAPRARSCPSK"
     gene            complement(12802..14196)
                     /locus_tag="VFV30_11285"
     CDS             complement(12802..14196)
                     /locus_tag="VFV30_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX4848719.1"
                     /translation="MSGPEETPVRRFLRSSGIMALFYAAAMVLAFVTGIVLTRSLGAY
                     GYGIYSLALTTATLIGLITEFGLPVLAMRETGTARASGKWGHLRGLLTWSDRAILLLS
                     LVLIAGTYGWIVLSGRAADSDYLAVMLWAVVLLPLVGLAKLRSTVLLALDRVAAGQFA
                     VMILRPLVFLAVCFAIPLFAGALTPAGAMIAQVLGAVASLVMVALLFRRWRPRELVEA
                     KPERDVRGWLAASLPMGLTEGLRLLQGQLALLLTGWLAGATAAGVYRGADAVLQLATV
                     IASVAATAATPMFARMVKEDDRAGIERIAALACLAMAGGVLGIALPIALVGDWLFPLL
                     FGQDFAASPPVFAILAAGLFATYGMGLAQALANMSGHHVLTTQSFLVTAAINLALGLA
                     LIPAQGAIGAAIATAVSATCGAGWCAWRLWRRTGYNTSLFNPALPGIVAGSVRSGLAL
                     AKGASRGQGNGIGR"
     gene            complement(14193..15266)
                     /locus_tag="VFV30_11290"
     CDS             complement(14193..15266)
                     /locus_tag="VFV30_11290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4848720.1"
                     /translation="MHAAFILPNMGAGGTERVTLDLMAGFLARGARVDLVLMERWGEF
                     LDQVPAEVRVIELGAPRLRQAITPLRRYFRSEKPDAALAAMWPLTTATVLAAAGLRPR
                     PRIVLADHAPLLDQYADNQGTLASLRATVRLSYRFADAIVCASEGLAGEMAALAGLPR
                     GRVSTVYNPIPEPPRSAGAPSPWPAGPGKRILSAGRLKLVKNFALLVEAFAPIAAEQD
                     ATLAILGEGEERGALEAQIARLGLQGRVLLPGYTATPGDWYREADLFALASDYEGLGN
                     VMVEAMHFGAAVVATDCRYGPREVLGGGRWGHLVPCGDAAALTGAMRAALAEPGDRAA
                     RIARARDFGLDPAVEAYWQAMSG"
     gene            15558..17525
                     /gene="asnB"
                     /locus_tag="VFV30_11295"
     CDS             15558..17525
                     /gene="asnB"
                     /locus_tag="VFV30_11295"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX4848721.1"
                     /translation="MCGIAGLLSAKAPVTPAVLTAMTDAMVWRGPDSSGAWVSEDGRI
                     GFGHRRLAIIDLSEAGHQPMHSACGRYTVTYNGEIYNYLDLRAELEAAGKAPQWRGHC
                     DVEVVLAGFAAWGIRKTLEKSMGMFALAVWDHRDRTLTLARDRFGEKPLYYGWTGAGL
                     AFASTLGPIKASPGFANPVADQAVACLMARAYVPAPLSIYRRVYKLPPGCLLTLSADA
                     AATPLDAPPAEGAGGPVRLERYFDYAEQVLAGAADPVSDREAELEELDAVLTRAVKRQ
                     LIADVPVGNFLSGGIDSSLITALAQKVVDRPIKTFTIGFSEAGFDEAVYAREVARALR
                     TDHTELYISPEDARAVIPSLPAMYDEPFADSSQIPTHLVSKLAREQVTVSLSGDAGDE
                     LFGGYTRHTQLPRKWKLAGWMPSALRRAAFETASAVPPGLWDAGSDLLRRRRSPWFGH
                     NARRGLRLLARADTFDALFDGLLNDWAYRASPLARSGPGHVPLQLDPRLAHLPLEVRM
                     MHADAVTYLPDDILAKVDRAGMAVSLESRIPFLDPEVTAFAARVSPSLKFARGGGKDI
                     LKQLLYRYVPRELVDRPKAGFAVPVGAWLRGPLREWAEELLSPQALGASGLFDAGVIR
                     TRWQAHLDSKEDATQPLWSVLMFQSWWDANR"
     gene            17522..18406
                     /locus_tag="VFV30_11300"
     CDS             17522..18406
                     /locus_tag="VFV30_11300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848722.1"
                     /translation="MSAAGTCFLCGSERLFRFPYLNMGSLGLVRGLAYKLPLSLGKGL
                     ARLSPRFARAWRPVETNRRYFDLTQCWCETCGTSWVEPWFEEARLSRYYEEYYWDNRD
                     EAEGRHLADDMRPNDLQRNWSADRIAWFSPHVPAFASAIDFGAGDCAAGYLLRDQAKV
                     IAVDPSTKAREIAARYGVEWRRTLAECPQVDLFYASHSLEHVHDLLAVFETMLGKVRP
                     GGHLFFEVPNVSSKEVLTKLCHTPHTFAFSKGSFEQLASRFGIRIVAMEDVGGPWQIP
                     GKTVSDPSKADLRVLFQR"
     gene            complement(18403..19128)
                     /locus_tag="VFV30_11305"
     CDS             complement(18403..19128)
                     /locus_tag="VFV30_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4848723.1"
                     /translation="MAETCALIAAAGRGTRAGLPYPKTLFEVQGKPILIRVAEALLPH
                     ADRLVVLASPDGAAPIRETLERHGIAADVVIQDEPRGMGHAVLTSAACPAVASAEHVL
                     LGWGDIPFFQPATVAAMMQAHRDHGNDLTLASALTDAAYTIVARDEAGQVVGLTETRE
                     AGLEPAPGERDIGLFVFRREPMFAVLRQDLPGKFGKGTGEHGFLYAIAPLAASGHRVE
                     AVPVATELDLVSLNSLGDLKGYI"
     gene            complement(19121..20023)
                     /locus_tag="VFV30_11310"
     CDS             complement(19121..20023)
                     /locus_tag="VFV30_11310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4848724.1"
                     /translation="MKILASVVTHNRRELLSRCIDRLQAQSRPADALLIVNNASSDGT
                     AEMLTARGVPFVTQDNLGSAGGWKRAIAHARENGFDAIWLMDDDGFPHGGALARLEAA
                     SGPQVSCAASVVLCEDGSGRFVFPFPHLNRNGHPAIVRFPRKLKSLDQLKPLAPAGTY
                     PFAHLFNGALIPLWAVERIGNVEDGYFMFGDEVDYFCRLRTAGQVVSVLDAVHFHPDV
                     SGRPYSPAKVYYYVKNSLILHRRYFDRAALRNLLALAAVLARVAARNGIGTALSYVAG
                     RQAGAFYGAIRRGLAGKVGKDFDG"
BASE COUNT         3348 a         6835 c         6891 g         3077 t
ORIGIN      
        1 ctcgggcgcg ctcgatcggc cggtgcagca gattggcctc accaccttcc gcccgcccta
       61 taccccgcag accttcgggg cgctggccgg gcacgccaag gacgcgctgt tccagccgac
      121 ccgcaagacc ccgatcgatg gttgggccga ggcgcacggc gcggtctatg agccggtcgc
      181 gctgtggcgc cgcgcgcgct acttccccaa gccgggcgag gacatgctgg ccgcggtcaa
      241 ccgcgaatgc ctggccgtgc gcaatgcggt ggggatcttc gacgcctcga cgctcggcaa
      301 gatcgaagtg gtcggcccgg atgccgccga gttcctgaac cggatgtaca ccaacccgtg
      361 gaaggcgctg gagcccggcc gctgccgtta tggcctgctg ctgaaggaag acggcttcat
      421 caccgacgat ggcgtttcgg cgcggctggc accggaccgg ttccacctga ccaccaccac
      481 cggcggggcc gcccgggtgc tgaacatgat ggaggactac ctccagaccg agtggccgga
      541 cctgaacgtc tggctgacca gcgtgtcgga gcagtggtcg gtgatcgccc tgcagggccc
      601 caaggcccgc gacgtgatcg cgcccttcgt cgaaggtatc gacctgtcgc ccgaggcttt
      661 tccgcacatg gccgtgcgcg aagggacgat ctgcggcgtg ccgacccggc tgttccgggt
      721 cagcttcacg ggcgaactcg gctttgaaat caacgttccg gcggcatacg gccgagccgt
      781 gtgggagcag ctgtgggagg ccgggcaagc gcacggcatc accgcctatg gcactgaaac
      841 gatgcacgtg ctgcgcgccg aaaagggctt catcatcgtc ggtcaggata ccgacggcac
      901 gctgaccccc tatgacgccg ggctggaatg ggccgtgggc aagaagaagc cggactttgt
      961 cggcaagcgc tcgatggcgc ggccggatat cgtcgcgccg ggccgcaagc agctggtcgg
     1021 cctgctgacc gacgatcccg ccgaagtgct ggaggaaggc gcgcagatcg ttgccgatcc
     1081 gaaccagccg gtgccgatgg acatgctcgg ccacgtcacc tcgtcctact ggagcgccac
     1141 tctcggccgt tcgatcgcgc tggcgatggt ggccggcggg cagggccgga tgggcgaaac
     1201 cctctacatc ccgatgccgg gccggacgat tgccgccaag gtgaccggca tggtgttcta
     1261 cgatccggaa ggagcgcggc tcaatggctg ataccctggc gcgcctggaa ccggtgccga
     1321 ccgagcccgc ggcctatccg ggtgtaacgg ttgcgctcgc tccgccgatg cggcgctggt
     1381 cgctgcgcgc gcgcgatccg aagctgctgg aaaaggcaat cggccgaaag gtgccgcgca
     1441 cgatcggcga gacgctggac ggcattgcct gccttggccc ggacgagtgg ctgtggcgct
     1501 cggccgaggg cgtgccggcc ggcatggccg agggccagcc ggtcagcatc gtcgacgtct
     1561 cggagcgctc ggttgccttt cggatcgaag gcccgctggc ccgggccgcg ctcaacgccg
     1621 gctgcccgcg cgacctggaa cggtttgccg tcggccagac ccggcgcacg gtttacgagg
     1681 gcgtcgagat catcctccac cgcgctggcg aggagcagtg gaccgccgag gtctggcgca
     1741 gctttgccga atggttctgg ctgtcgctca acgcggctgc cgctcacccc tagaccggtt
     1801 tcacccggca gctgtccggc aaggctggca gggccgggat cgcccgctcc atccgcgcgg
     1861 ccctctctcg cgcgctgcgg tgggttccgc tgccgcccag gaagggatcg aaccgcaccc
     1921 cggtgcggcg caggaacttc ggcgcaatgg ccggatccca gcaggccagc cgcagcaggt
     1981 ccaggctcgc cagatcggcc tcgtcctcga actgccgggc aagggcggtc cgctggcggc
     2041 gcagggcggg gcctggcgcg gcctgttcca gcggggccag ctggcggcgg tgatcgagca
     2101 ccgtgtgggc cagttcgtgg gccagcgcgg cggccagccc ttcgtcgtcg agcgattcgg
     2161 caaaggcttg gccgatctgg ataatccggc cgtccgagcg ggccttgagg gcgctgcccg
     2221 cgaccacttc gatccgggtg gcacaggtgg ggaccggcac cagcaccgtc tccagccagg
     2281 cctcgccgcg ccggacccgc agccggatcg gtgcggacgg gggcagggcg agcagccggt
     2341 cctcggccgc atcgcgcagc gcggttgagc cgctgccggg cggagcggag gctggcagag
     2401 gctcgccgtt gatcgccgcg atcccgtcgc cgccgcgcag gtcggcctgc gcggcggggc
     2461 tgcccggaac gacatcggcc accgcaaccg ccgtgggaaa ggcccgcagc gcctgcactt
     2521 cggcccgtgt tccggcggaa tactggctga gcgaatggag cagcagcccg gtgcccggca
     2581 tctgccgcgc gcagcggggg ctggccgcgg tgactaggcg gaatccgctg gccgcaaccc
     2641 gcaggtcggt ggcgaacaac cggtccagaa tgccgcctgc cgtaacggca gacgcaggtg
     2701 aaaaggccgc caatccggcc agaacggccc aaaacaggtg tctcaccata gcgggggagc
     2761 ctaatcccgt gcagccggac ggtccagccc aacggttcac cgcattttcg ccgccaaatt
     2821 caccctaaac gcccgggatt gcagggctag tttacggttt tttgccaatt tccctgcaca
     2881 tggcaaagtt gaatcaagca gattcagtta attgcattga aagcgattcg ctgttaatgg
     2941 ggctctcgac cttccggatg atcccccaag ttggagtctt aagccgatga cgatgaccaa
     3001 gaaactgatg gccgccgccg cggctttcgc catgaccgcg acgcccgtgc tcgccgccgg
     3061 cgaaacccag gctgccccgg ttcgcgacgc cgctccggtt gctcagtcgg agcagctgtt
     3121 tgacggtgac ggcggtggct ggctgctgtt cgtcctgctc ggcgcggctg ccgttgttgg
     3181 cctgatcgtc gccatggacg atcccgccag cccctgagct gacgctgcga tcggttgagc
     3241 ggccgcaagc cgttcttccg tgacagatga attcgaatga gccggagcgc cccaagcgct
     3301 ccggctcttc tcgtttgtgc cggtgtttga tgtctgccgg gtgcggatat cgccccggca
     3361 gaccggcgaa gtctagatct gcagcatggt ctcgcgcttg cctgccccgg gcgaggactg
     3421 ggcgagggcg gccgaaccgc gcagggccgg cgggcggcag gcaaagatgg cgcagagcgc
     3481 gatcaggcag ctgaccgcgg ccgtgcgcag cggatagtcg accaggctgt gggtcaggac
     3541 cagggccgtg gccagcgtgg ccgccagggc gaccggatgc tcggtgcgcg gagcgttcca
     3601 cagctgccag ctcttgccgc cgatccagcg caggaacagc gccagcacga tcaggccggg
     3661 gatgccggct tcgatcacca gttccagcag atcgttgtgg gcgtggttga cgaaggtggt
     3721 gctgacattg gtggcgcttt cgtaccacgg gtagagatcg cggaaggtgc cgatgccggc
     3781 acccagcggc gcgaaatcac gggcaatcgc gctgccgttg gcaagaaact cgccgcggct
     3841 gatcccggcg gtgccggact tgctcatcaa gtcgttggag acggcgccgg acgcaagcag
     3901 ccagacgatc cccaccgcaa atgccaaggc ggcaatgccg atcagcggcc agcgtatccg
     3961 ccactgcgga tagacgacca gcgcgatccc gccgagcagc ggcaggagca gcaggccggc
     4021 ggcgagcgag cgggacagca gcacgcccag caccagcggc gccagccgca gcagcagttc
     4081 aacattgagc gagatgcgcg gtgcaatcgg ccgacgggtc ggccggtcgt gcagccactg
     4141 cccggcggcc agagccgtcg cgatcagcat gaagctgccg aagtggttgc tgttggagaa
     4201 caggccaacc ccgttgccgc ggttggtgat ggcatagaaa tagcccgaac cggacaccac
     4261 ctgcatagcc gccagcacga ccgaggcata ggccacggcc agaatgaccc agatggcatg
     4321 gcgggtctcc gggctgcttt ccgcccgcat ggcatagaac agcgcccctg ccgggatcca
     4381 ccagaccagg ctttgcaggc tgctccaggg atcgagcgaa tagctgagcc agggcagttc
     4441 cgatccggtc agggcaaagc cctgggccac ggcctcgcgc cccggcagca tctgccacag
     4501 cgccggtggc agcggcagga actggatcag cgccagcacg gccaggccaa tcatgaaagc
     4561 gccggcgccg gtgcgcaggc ccttgccggg ctcctcgctc cacagggtcc aggcgatcag
     4621 gccggccccg gcgatctgca acaccaggtt gccgccaaag cccgccgcgc tggctccgcc
     4681 cagcagcagg atcaacagca ggtacagcag cgtggcggcc tgccgcagcg taatgccgag
     4741 gttcattgtt ggcctcctgc gggaacgaca tcgatccggc gaaccgagac ctgcatttcg
     4801 ccgcgcttct ggctggccgc gccgaacagc gcgaggatct gtccgttgca gccctgggcc
     4861 ggcacggcaa agggcagcgc aaccgagcgg ctgccctgcc gcagcgggat ttcggcgagg
     4921 tagccggtct gtccgtggca cgaaacgcgc agccgcacgt tgtcggcccc gccgctgatc
     4981 acttcgaatt cggccgtggc gcgatagctg cccggtgcca gggtcagcag ctgggaaacc
     5041 agcgcgccgg gctgccggtc gtaataggag acatagattc cgccgtcctt gctccgctcg
     5101 gccgcgcccg tggtgttcac ggacagcaac cagttgaacg gggccggtgc gcgcgcatcg
     5161 ctgaagtcag gggagtggac cagtccgcca ccggcggcca tgccccggct ccagatcgtc
     5221 cgggcctggc tgaacgcgcc gacggccacc agatgcttga tcaggttctt gcgggtatcg
     5281 ggatcgtcga gcacccgcgc gggcaggcgt tcggccagcc gcacgaccac ttcgggggtc
     5341 ttttccttgc cgcccatgct ggccaccagc ggcaggtaca ggctgtcatg cccgctgatg
     5401 gcatccagcg cctgatcgac attgagcggg gtggcgatcc gctgcgcgat cgagcccatc
     5461 accacatcga ccagctgcgg gttgagccgc tgcaggatgg ccagttcctg aaaggccggc
     5521 ttcagttctc cgctcgccgc cagctgccgc aggcgcagga cccgcgccgt gcgcgaccgc
     5581 ggatcgcggc gcagcgcttc gagcagcatc ttgtcctcgt tgccgatcgt gcccttggga
     5641 ttgtcacgca ggccaaggaa cgcgaagccg atgaacgggt cggccgagag cggcgagctg
     5701 agcagggccc gctgggccag atccttgtcc agctcaggct ttccggttcc gagcttggac
     5761 agctcaaacc gcagcgaacc cagcgccccc gggccaagcg cgctcagctg cgaaccggcg
     5821 ccggccgccg gcgtttcggc cacccgcgcg gtgttcgcgg ccagcacggc ggcggtcaga
     5881 gccgccaaca cgccgcctgc cagaagtttg cgccgcaccg attgaaacgt gatgccgccc
     5941 ggcatgatgg aactccgcat caactcttgg ccacatcaat ccggcgggcc gagatcgtat
     6001 cgtcgctgtc gttgtcgtcg ctgtggctgt agtggccgta gccgtaacca tagccgtagc
     6061 catagccgct gcgcttgacc gttaccttgg tcaggatcac gccaaccagg tgagcgccgg
     6121 catcggccag acggcggacc atttcctgaa cctcggccgt gcgcgcgccg cggcattcca
     6181 ccaccagcgc ggtggcatcg acaaccgaag cgagcagcgg ggcatcggcc aggcccagca
     6241 ccggcgggcc atcgatcacc accatgtcaa agaccccctg ggcctccttc accagcagcg
     6301 gcaggcggtt agagctgagc agctcggcag ccgatgcgct gatccggcca accggcagca
     6361 gcgaaaggtt cggggtcttg gtggcctcga tatagtcgcc cagcgggtcg tctgacacga
     6421 gcaggtggcc cagccccatg ccgtccttgc ggcccgaggc gaaggtcggc ttgcgcaggt
     6481 cggcgtcgat caggagcacc ttgctgccaa gcttggccac gctgagcgcg atggcatagg
     6541 acgaggtcga cttgccttcg cccggccggg tcgaggtgac cagcagggag tggggcaggc
     6601 cttcgggccg cgcaaacttg agcgccgttc gcaccgaatg gtaggcttcc gacatctgcg
     6661 atttgctgtc ggccagcgcc tcaaccgggg cgcgaccgtc ggtttcgacc gggatcgcgc
     6721 ccagaaccgg caggcgcagc ttggtccgca cgtcgcgggt gtcggtgatc gagtcgaaca
     6781 ggatctggag cccgagggcg agacccacgc caagcgcgag gccggccagc aggccgacga
     6841 tggcgttgac cagcggacgc gggcggaacg ggccggccgg aaccttggcc tcgtcgacca
     6901 tcgagatctc gctcttgccg atgccgccgg ccacgccgac ttccttgtag cgctgcagca
     6961 gcgcatcata gagcgagcgg ttggtatcgg tttcgcgctg caggatgttg tattcgatcg
     7021 cgatgttgcg atcggccagc acttcgccct tggcttcgtt gaccgcaacc tgcagctccg
     7081 cttcggtctg cacggcggcg cggtattcgc catagagttc ggcccgcttg ttggtccgcc
     7141 cccgcttgcg ttcggcgttg atcgccgcgt cgatccggtc aatccgggcc ttgagctcga
     7201 tcatcgacgg gtagtccggc ttgtagatct tcagcttctc gtcgtactgc gcctgcagtt
     7261 cggtcttctg ctggatcagc ggcgacaggc tgagggcctg atccaggctg ccgtccagtt
     7321 cggcttcctt gtagcgctgc tccgccgcga tccggttgac cttggcccgg gccagcgcct
     7381 cgttcagcga ggagagcttg gactgggaca gcgatgtccc ttccacttcg cgcccatctg
     7441 cggcgcgggt ggtgttgcgg aacacgccgg ccttgatcgc gtaattgttg aggttgcgct
     7501 ccgagacttc cagcgattct ttggtccgcg cgatctgatc agagaggaaa tcgcgggcat
     7561 aggacgagga ttcgaaccgc cgcgccaggc tggccgagat atgccccttg accaccgcgt
     7621 tggcgatctg ggccgcggtg cggggatcgg tcgatacggc gctgatccgg atcagcgagc
     7681 tgcccttgat cggttccgca gtaatcgaac cctggacggc cgcggccgcg ttgttggtgc
     7741 gctggtcgat cgtgccgccg gcgccgccat aggcgggatt gctgacgagg ttgaggtcct
     7801 gcgccacctg ccgggccagc gattcgctct gcagcaggcc gatctgggtc aggatgcctt
     7861 cctgggtgtt gcccttcgcg ctgcgctggc cggcgacata gcggcccggc tccagcaggt
     7921 ccgccgcgcc cggttcgtac tggatcagcg ccgaggcgcg gtacagcggg gtcgccatca
     7981 gggcggccac gatgccgagc agcagcccgg ccgcgcccat cgccaggatc agcttgtagc
     8041 ggtccagcag gatcgaaagc aggtgcgaga gatcgaaccc gtgttcggcc gcgtgcggat
     8101 gccccgcgcc ggtggagggg tcgtcaccct ggatggtgtg gccttgccgc atgtctgacc
     8161 tttggttttc ctgaatgttt tgccgaaagc ctggacaagg ctagagagcg aggaacagcg
     8221 agaggatcgg gatcgacatc aggacatcgc ggtaggcggg gcgcagctgc gagctgtcga
     8281 ccaccacccg gtcgcccgga tagatatcgg gatcgtccat ttcgccgcgc cggatcgaga
     8341 ccagatcgaa ggccgcggcc agcgtcttgc catccttctt gcggaagatc gcaacccgcc
     8401 gcggattgcc gtcgttggtg ttgatcccgc gcgccatcgc catcaccccg agcagggtgg
     8461 tgcggcccgg caggacatag accccggggg cgttgacgcc gccttccacg gtcacgctgt
     8521 tgttgttggt gctgaggatc cggacggtga tttccgggtt gttgaggtac ttcgcggcgt
     8581 agcgcttctc cagctcgtcc gcgaactggg tcggggtcac gtcacgcacg ttgatcagcc
     8641 cgagcagcgg catgtcgacc atgccgcggg cgtcgacctg gtagtcggag ctcagttcgg
     8701 ggacccggaa cacgctgacc cgcaccatgt cgagcgggcc gagcggcaga tcgtagacaa
     8761 tgtcctccgc ggcctgcttg tccggcgggc cgaagtcggc cggggcatag gggatcttgc
     8821 cgccgcgcgc cgaacaggcg gcgaggccca gggccatcac ggccagcacg atcagccgaa
     8881 acgccgattg aaagccgctt tgggaagccg atcgcgaacc caatgcttgt ctccttggat
     8941 cgggtgtatc ttgccctgcc ggctgggcgg cagcgctggg aaacccgggt tctaatgcat
     9001 agttgggcct atgcaaaggg ttctcgcgtc ccgatcatgc cgtaactggg ggtaagattc
     9061 cggatcggga cagggcggca agatattgtc ctgtcaccat ttcctgtgag aattcggcca
     9121 ggacccgggc gcgcccggcc tggcccatcc gcagccagtc cgcatccccg gtggcggccg
     9181 cctgttccag cgccgcagcc agcgcggcgg catcgcgcgg ggcgcagaga aagccgtttt
     9241 cgccctcgcg gacaatgtcg cggcaaccgg tgacgtcggt ggtgacgatc gggcgggcca
     9301 tggccgccgc ttccagcagg acgcgcgaca ggccttcgcg gtaggacggt aggacgacaa
     9361 agtccgccgc ggcgatttcg gggcggacat cgtcgaccgg ctcggcatat tcgatcgcgc
     9421 cctccgcgat ccagccgcgc acttccgctt cgggaatggc ggtgcggttg gccgcgccca
     9481 gcgcgcccat cagccggaac cgtgcatccg gccagcgggc ccgcacgcgg cgcgcggctt
     9541 caacgtattc gcgcacgccc ttgtcggcga ccacgcgggc cagcatcaga aaggtccgcg
     9601 gcggcggccg ctcgctgctg gccggggccc aggcggccgt gtcgatcccg gaaccgggca
     9661 ggatggcgat ctgctcgggc cggaccagcc ggttctgcag gaacagcgcg ggatcgtccc
     9721 ggttctggaa gaacacggtg ggcgccttgc gaaagccggt gcggtacagc tgctgcgcga
     9781 tcacggtcag cacgttgcgg cggataaagg cggtgcccag gcccgagacg ttgggaaagg
     9841 ccggcactcc ggccaggccg gcggcaagcg agccgtagac attgggcttg atcgtccagc
     9901 tcagccaggc cgccgggcgg tgctgggcaa tcagccggcg aaaggccagg aaagtggcga
     9961 tatcgcggtg cggggcaagc cccatcgcgt caatcggtac cgccgtgaaa tcggcgccta
    10021 gggcgcgcag ccgggcttcc atgtccggat cggccggggc cgcggcgagc acccggtaac
    10081 cggcttcgat caggcccttg atcagcccgg agcggaagtt gacgagattc caggccagat
    10141 tggcgcagac gatcaccaag ggggcatcgg gccggcgctc catcggctca gatcgcctcg
    10201 aacagcgtct tgtagggcac ccagaactcg gcgttggtgg catagaccag ccacaccacc
    10261 tggaccgccg aggcgacccc gatcagcagg acccggacga acgcggtcat ccgttccggc
    10321 gttggcagaa ggttccggaa ctcgccgaac accagaagct gggcgatcga gaagtacagc
    10381 ccggcgcggt ccactgcggt cgaggagggg ctgacgaaca ggagcagaag cagcagcaca
    10441 ttggcaatcg ccaccgagat ccagattggc cggagccggg atgaaccgcc aatatggcgc
    10501 cagcgtagca acagcagcag cgaggggatg aagctcatca gcagacgcgc cagcgcgccg
    10561 gtcgattcca gttcctgatc gatgtagccg gcattgaacc gatcaagcgc cggaacgaca
    10621 atggcgaagt aggcatagat cgtcgccagg ccgaccacga ccgcgaacag gcggtaccgc
    10681 gtgcccaggc tgtagccgaa cagcggaatg accatgatcg cggtcgcgtg aaagccgatc
    10741 gccatcagca gaaagacccc ggtccggatc aacttgcccc tgtcgaacgc tgcgatggcc
    10801 atcagcagca ggccaatcgc ggcgccctgg cgaatgtagc caagacccac cacgatcagc
    10861 agatagggca ccgccatcag cacggcgagc cagggatcgc ggaagcgtcc gacaacctgc
    10921 gccacgccca ggacaagcag gaacgcgcag accccgttga cgaagtagat gtccagatcg
    10981 aactgggcgg agacccagtt gaccaatccg tagagcggat cggtcacgcc catggcatag
    11041 ctcaggctgc tgagtgcgat gtcgtcgtag atttcctcgt aattgcccca gtcgccgccg
    11101 acgtcccagc gcatgccggc catcagcgtg tagaacagca caaagcccgc aagcgcgaga
    11161 acctgtcccg cgctttggcg agtctgctcc gtccgggccg gataggccag cgccatcacc
    11221 gcagggtagg caagaaggag ccagtacatc agcatttcag gggcggcctc ctcgcgcgcc
    11281 gtttcgcggc atgggccgct tatcgccttt gcgcatcggg ctcaaccgca gcggcctcaa
    11341 ccggcttgac cagccgcgcc ggtgcgccgg catagacccc gcgaaccggc agcgagcggg
    11401 ttaccaccgc attggcaccg accacggcat gcggggcgat ctccaccccc ctggtcacgg
    11461 tcaccttggc ggcgagccag acatcgtggc cgatccggat tgccgcagtg ctgctgccct
    11521 gcgtctcgag ccggccctct ccggcgtaat tgtggtcctg gtcgcggatc gtcacgtgtt
    11581 cggccgtcag cgtgccgtcg ccgatttcga tcgcttcact gcagaccacg accgcagctc
    11641 ggccgatcag gcagttccgg ccgatcgtca gactgccgcc ttccacctga atccaggcgt
    11701 gttcgagcac gaccgtgcct tccccgatct caagccgcgc cccgtcaaag gcgcgcacgg
    11761 tcacgccctt gccgaagatc accgagcggt ggcagcgaac cccggggtaa gccagccgca
    11821 aataggtgcg gaacgccgcg ctgcgcaggg cccagtgggc tttgcgcaac ccggcgaaaa
    11881 agcgtggaac cagcctcatt gcgctgcctt cgggttggcc ccggcgagcg gccgggcaag
    11941 gaaatcaaac gcgtcgagct gttcgacggc gaagcctgcg gagtgcagca gggcgaagat
    12001 ccgggcatcc cggtcattgc tttcgaaaac cacacagccg gtccgcaaaa ggacctcccg
    12061 cgcaccgctc agcgccatga attcagcccc ttccggatcg agcttgatca ggtcaatccg
    12121 gccgagatcg gcgagcgcct cgtcaagagt gaccgtctcg acgctgacac tgcgccgcgc
    12181 gtcctgatcg ccggcaacga tcgaggcctg gccgtgcttg cccggctcga cgctggcggt
    12241 gacccgatcg cctgaacggt cggacagcgc gcgctcgatc accgtgaccg gcagcggtcc
    12301 gttgtcggcg acatggcggc gcaggatcgc agcggtatcg ggcatcattt cgaaggacac
    12361 gaccttgccc tcggcgccaa cccttcgcgc ggcaaccagc gtgaagaatc gatactggcc
    12421 ccgccatcga cgaaagttcc gcccggcggc agttcagcct cgatcacgcg ccgcacgtgg
    12481 ggctcgcgcg aggtcatcac gtgcagcaga tcgtcggtcc cggcgcggac cgcgaagcgg
    12541 ccgacacccg gcaccgccac ggcgatgtct ccgatcagat gcggctggcg gtagctgtcc
    12601 agcgcccgca gcgaagtgaa gggcgcggcc agcgccgaca gccacaacac ccttcggctg
    12661 cgtccatcac cgggttggag ctcgcgcagc aagttgagcc acatccgcag gcggtagggg
    12721 taattgagcg ccatgcgcac ggccttgcgc aggattcccg gcgttctcgc cgcgatcctt
    12781 tgtcgcaacg gcaggttcgg tttatcgtcc gataccattg ccttgacccc tgcttgcgcc
    12841 cttggcgagc gccagtccgg accggaccga ccccgccaca atccccggca aggccgggtt
    12901 gaacagactg gtattgtatc cggtgcgccg ccacaatcgc catgcgcacc agcccgctcc
    12961 gcaggtggcc gagactgccg tcgcgatcgc cgcgccgatg gcgccctgcg ccgggatcag
    13021 ggcgagcccc agcgccaggt tgatcgcggc ggtgaccagg aaggactggg tggtgagcac
    13081 gtgatgcccg ctcatattgg cgagcgcctg ggccaggccc atgccgtaag tcgcgaacag
    13141 ccccgccgcg aggatcgcga agaccggcgg cgaggcggcg aagtcctggc cgaacagcag
    13201 cgggaacagc cagtcgccca ccagcgcgat cggcagcgcg atgcccagca ccccgccggc
    13261 catggccagg caggcgaggg cagcgatccg ttcgatcccg gcgcggtcgt cttccttgac
    13321 catccgcgcg aacatcggcg tcgcggcagt cgcggcgacc gaggcgatca cggtggcgag
    13381 ctggagcacg gcgtcggccc cgcggtagac gccggccgcg gtggccccgg ccagccagcc
    13441 ggtcagcagc agcgccagct ggccctgcag cagccgcagg ccttcggtca ggcccatcgg
    13501 caggcttgcc gccagccagc cgcgcacatc gcgttcgggc ttggcttcga ccagctcgcg
    13561 cgggcgccag cgccggaaca gcagcgccac catcaccagc gatgcgaccg ccccgaggac
    13621 ctgcgcgatc atcgcacccg caggagtgag cgcgcccgcg aacagcggga tggcaaagca
    13681 caccgccagg aaaacaagcg ggcgcaggat catcaccgcg aactgcccgg cggcgacccg
    13741 gtcaagcgcc agcagcacgg tcgagcgcag cttggcgagc ccgaccagcg ggagcagcac
    13801 cactgcccag agcatgactg caaggtaatc ggaatcagcg gcgcggccgg acagcacgat
    13861 ccagccataa gtcccggcga tcagcaccag cgacagcagc aggatcgccc gatcggacca
    13921 ggtcagcagg ccgcgcaggt gcccccactt gccgctggcc cgcgccgttc cggtttcgcg
    13981 catcgccagc accggcagcc cgaattcggt gatcaggccg atcaatgtcg cggtggtcag
    14041 cgcaagcgaa tagatgccgt agccataggc tccgagcgag cgggtcagga cgatgccggt
    14101 gacgaacgcc agcaccatcg ccgcggcata gaacagcgcc atgatccccg acgaacgcag
    14161 gaaccgccgg acgggggttt cctcggggcc gctcatccgg acatcgcctg ccaataggct
    14221 tcgaccgcag ggtccagccc gaagtcccgc gcacgggcga tccgggcggc gcggtcaccg
    14281 ggttcggcca gcgcggcgcg catcgctccg gtcagcgccg cggcatcgcc gcaaggcacc
    14341 aagtgccccc aacgcccgcc gccgagcact tcgcgcgggc catagcggca gtcggtcgcg
    14401 accaccgccg cgccgaaatg catcgcctcg accatcacat tgccgaggcc ctcgtagtcg
    14461 gaggcgagag cgaaaagatc ggcctcgcgg taccagtcac ccggggtcgc ggtgtagccg
    14521 ggcagcagca cacggccctg caggccaagc cgggcgatct gcgcttccag cgcgccgcgc
    14581 tcctcgcctt cgcccaggat cgccagcgtc gcatcctgct cggccgcgat cggagcaaag
    14641 gcctcgacca gcagggcgaa gttcttgacc agcttgagcc gaccggccga gaggatccgc
    14701 ttgccggggc cggcgggcca gggcgagggg gcaccggcgc tgcggggcgg ctcggggatc
    14761 gggttgtaga ccgtgctgac ccgcccgcgc ggcaatccgg ccagcgccgc catctcgcca
    14821 gccagtccct cgctggcgca gacgatcgca tcggcgaagc ggtagctcag ccggaccgtg
    14881 gcgcgcagcg aggcaagagt gccttggttg tcggcatatt gatcgagcag gggggcgtgg
    14941 tcggccagca cgatccgcgg acgcgggcgc agaccggccg cagccaggac cgtcgcggtg
    15001 gtcagcggcc acatcgcggc gagcgcggca tcgggcttct cgctgcggaa atagcgccgc
    15061 agcggcgtga ttgcctgccg caggcggggc gcgcccagtt cgatcacccg gacctccgcc
    15121 gggacctgat cgaggaactc gccccagcgc tccatcagca ccagatcgac ccgcgcgccg
    15181 cgcgccagga aaccggccat caggtccagc gtcacccgtt cggttccgcc cgcacccatg
    15241 ttcggcaaga tgaaggcggc gtgcatcacc gctcagaccg tttgcggctt tcgcgcgaag
    15301 gcaattcggt tcaacgtcac cccatcagcc tctagccccg gtcccgggca atgcaaaccc
    15361 ctgaccagcc gtaaacgcct gtcacgggca gcaaattcag gttgatcgcc cctgcgccat
    15421 cccataacga ccgggcatga tctccccgca acgcaccctt gggcagctgc gccctttttg
    15481 ccagcaaatt cagggagatt cgtttttcct cgccggcggc cgtcccgcgg cctccgccgg
    15541 gaaggggggc tgagccgatg tgcggtatcg ctggcctgct gagtgcgaag gcgcccgtca
    15601 ctcccgccgt attgacggcg atgaccgacg cgatggtctg gcgcggtccg gattccagcg
    15661 gtgcatgggt cagcgaggat gggcggatcg ggttcggcca ccgccggctg gcgatcatcg
    15721 atctcagcga ggccgggcac cagccgatgc attcggcctg cgggcgctat accgtcacct
    15781 acaacggaga gatctacaac tacctcgacc tgcgtgccga gctcgaggcg gccggcaagg
    15841 cgccccaatg gcgcgggcac tgcgatgttg aagtggtgct ggctgggttt gccgcctggg
    15901 gcatccgcaa gacccttgaa aagagcatgg ggatgttcgc gctggcggtg tgggatcacc
    15961 gggaccgcac tctgaccctg gcgcgcgacc ggtttggcga aaagccgctg tactatggct
    16021 ggaccggggc cggtctggcc tttgcctcca ccctggggcc aatcaaggcc agcccgggct
    16081 tcgccaaccc ggtggcggac caggcggtcg cctgcctgat ggcgcgggcc tatgttcccg
    16141 cgccgctttc gatctaccgg cgggtctaca agctgccgcc gggctgcctg ctgaccctct
    16201 cggccgacgc cgctgccacg ccgctcgatg cgccgccggc ggaaggcgcg ggcgggccgg
    16261 tgcgtctgga acgctatttc gactatgccg agcaggtgct ggccggggcg gccgatcccg
    16321 tttccgatcg cgaagcggag ctggaggaac tcgacgcggt cctgacccgc gcggtcaagc
    16381 gccagctgat cgccgacgtg ccggtgggca atttcctgtc cggcgggatc gattcgtcgc
    16441 tgatcacggc gctggcgcag aaggtggtcg atcgcccgat caagaccttc accatcggct
    16501 tttccgaggc cgggttcgac gaggcggtct atgcccgcga agtggcccgc gcgctgcgga
    16561 ccgatcacac cgagctctac atctcccccg aagacgcccg cgcggtgatc ccttcgctgc
    16621 cggcgatgta cgacgaaccc tttgccgaca gctcgcagat cccgacccac ctcgtctcca
    16681 agctggcgcg cgaacaggtc accgtttcgc tgtcgggcga tgccggggac gagctgttcg
    16741 gcggctacac ccggcacacc cagctgcccc gcaagtggaa gctggcgggc tggatgccat
    16801 cggccctgcg ccgcgccgct ttcgaaaccg ccagtgccgt gccgccgggc ctgtgggatg
    16861 ccggctcgga cctgctgcgc cgccgccgct cgccgtggtt cgggcacaac gcccggcgcg
    16921 gcctgcgctt gctggcgcgc gcggatacgt tcgatgccct gttcgatggc ctgctcaacg
    16981 actgggccta ccgcgcctcg ccgctggccc gcagcgggcc gggccatgtc ccgctgcagc
    17041 tcgatccgcg gcttgcgcac ctgccgctgg aagtgcggat gatgcacgcc gatgcggtca
    17101 cctatctgcc cgacgacatc ctcgccaagg tggaccgggc gggcatggcg gtgagcctgg
    17161 aatcgcggat tccgttcctc gatcccgaag tcaccgcttt cgccgcgcgc gtctccccca
    17221 gcctcaagtt cgcccggggc ggcggcaagg acatccttaa gcagctgctc taccgctatg
    17281 tcccgcgtga gctggttgac cggcccaagg cggggttcgc ggtcccggtt ggcgcgtggc
    17341 tgcgcggtcc gctgcgcgaa tgggcggaag agctgctcag cccgcaggcc cttggcgcca
    17401 gcggactgtt cgacgcgggc gtgatccgga cccgctggca agcgcatctg gatagcaagg
    17461 aagacgcgac ccagccgctc tggtcggtgc tgatgttcca gtcctggtgg gatgccaacc
    17521 ggtgagcgcg gccggcacgt gtttcctgtg cgggtcggaa cgcctgttcc ggttccccta
    17581 cctcaacatg gggtcgctcg gtctggtccg ggggttggcc tacaaactgc cgctgagcct
    17641 cggcaagggc cttgcccggc tgagcccgcg gttcgcccgc gcctggcggc cagtcgaaac
    17701 caaccggcgt tacttcgatc ttacccagtg ctggtgcgaa acctgcggca ccagctgggt
    17761 cgagccgtgg ttcgaggaag cgcgcctgtc gcgctactac gaggaatact actgggacaa
    17821 ccgcgacgag gccgaaggcc gccacctggc cgatgacatg cggcccaacg atctgcagcg
    17881 caattggtcg gccgatcgga tcgcgtggtt ttcgccccac gtcccggcct ttgccagcgc
    17941 gatcgacttt ggcgcgggcg actgtgcggc gggctatctg ctgcgcgacc aggcaaaggt
    18001 gatcgcggtc gatccctcaa ccaaggcgcg cgagattgcg gcgcggtacg gggttgaatg
    18061 gcgccggacc ctggccgaat gcccccaggt cgacctgttc tacgcctcgc actcgctcga
    18121 gcatgtccac gacctgctgg cggtgttcga gaccatgctg ggcaaggtcc gccccggcgg
    18181 acacctgttc ttcgaagtgc cgaacgtctc ctcgaaagag gtgctgacga agctttgcca
    18241 caccccgcac accttcgctt tcagcaaggg ctcgttcgag cagctcgcca gccggttcgg
    18301 catccggatc gtggcgatgg aggatgtcgg cggcccgtgg caaatcccgg gcaagaccgt
    18361 cagcgacccc agcaaggccg accttcgggt gctgttccag cgctaaatat agcccttcag
    18421 atcgcccagg ctgttgagcg agacgagatc gagctcggtc gccaccggca ccgcctcaac
    18481 ccggtggcca gacgccgcga gcggggcgat tgcatagaga aagccgtgct cacccgtacc
    18541 cttgccgaac ttgcccggca ggtcctggcg caggacggcg aacatcggtt cgcggcggaa
    18601 aacgaacagc ccgatatcgc gctcacccgg ggcaggctcc agcccggctt cgcgggtttc
    18661 ggtgaggcct accacctggc ctgcctcatc gcgcgcaacg atggtatagg ccgcgtcggt
    18721 cagggccgag gccagcgtca ggtcgttgcc gtgatcgcgg tgcgcctgca tcattgcggc
    18781 gaccgtggcc ggctggaaga acggaatatc gccccagccc agcagcacgt gttcggcact
    18841 cgccacggcg gggcaggccg cgctggtcag gacggcgtgg cccatgccgc gcggctcgtc
    18901 ctggatcacc acatcggccg cgatcccatg ccgctccagc gtctcgcgga tcggcgcggc
    18961 accgtcgggg ctggcgagca ccaccagccg gtcggcatgg ggcagcagcg cttcggccac
    19021 gcggatcagg atcggctttc cctgaacctc gaacagggtc ttgggatagg gcaggccggc
    19081 gcgggtgccg cggcccgcgg cggcgatcag ggcgcaggtt tcagccatcg aaatcctttc
    19141 cgaccttgcc tgccaggccg cgccggatcg cgccatagaa ggccccggcc tgccgcccgg
    19201 cgacatagct cagcgccgtg ccgatcccat tgcgcgcggc cacgcgggcc agcactgccg
    19261 ccagcgccag cagattgcgc agcgcggccc ggtcaaaata gcgccggtgc aggatcagcg
    19321 agttcttgac gtagtaatag accttggccg gggaataggg gcgcccggag acgtcgggat
    19381 ggaaatggac cgcatcgagc accgagacga cctggcccgc ggtgcgcagg cggcagaagt
    19441 agtccacctc gtccccgaac atgaaatagc cgtcttcgac attaccgatc cgctctaccg
    19501 cccacagcgg gatcagcgcg ccgttgaaca ggtgggcaaa gggataggtg cccgccggag
    19561 caagcggctt cagctggtcc aggcttttca gcttgcgcgg aaagcggacg atcgccggat
    19621 ggccgtttcg gttgagatgg gggaagggga acacgaaccg gcccgagccg tcctcgcaaa
    19681 gcaccaccga cgcggcgcag gacacctgcg ggccggatgc ggcctcgagc cgggccaggg
    19741 cgccgccatg gggaaaacca tcgtcgtcca tcagccagat cgcgtcgaag ccgttctcgc
    19801 gggcgtgggc gatggcgcgc ttccagccgc cggccgaacc gaggttgtcc tgggtcacga
    19861 acggcacgcc gcgcgcggtc agcatttcgg cggtgccatc gctgctggcg ttgttgacga
    19921 tcagcagggc gtcggccggg cgcgactggg cctgcagacg atcgatgcag cgcgagagca
    19981 gctcgcgccg gttgtgggtg acgacggagg ccaggatctt catggcgggc cggactcgcc
    20041 cgaggacggc gaccgccggg cgaaccgcga tcccgacagg gcatagcggc tggtgccaaa
    20101 cagcagtccg gccacggtat aggcgttgcg ggccaggcgg taggccgcgg t
//
LOCUS       DASUDV010000518         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2005758,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000518 DASUDV010000000
VERSION     DASUDV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005758"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..558
                     /locus_tag="VFV30_11315"
     CDS             88..558
                     /locus_tag="VFV30_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX4848725.1"
                     /translation="MGTPASINPRIVEGLYCEALVLSDEVRAAFALSGRLDAPAAGDH
                     GSDEARIALSCEALRTTTRMMHALAWLLNHRAYFAGEISEFQLRRHGKLAESPEAEPE
                     RLALLDPEIRSLILSTERFHARLKRLDSGWRDREPGAPSALAALRERLGLQVRN"
     gene            604..1527
                     /locus_tag="VFV30_11320"
     CDS             604..1527
                     /locus_tag="VFV30_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4848726.1"
                     /translation="MNIPPKPWPTGLSELCEPLVRRVLAPNASHYTFTGTQTYIVGAG
                     DEVAVIDPGPADSGDEADPSFFKGADTNGRGHVEAILAAVGAARIGAILCTHTHRDHS
                     PAAAVLKARTGAPIIGCAPLVLADDGPRADSAFDQFYSPDRVLADGERVSGPDWTIEA
                     VATPGHTSNHLCYALVESGALFTGDHVMAWSTSVVSPPDGDMAAYMASLQKLYDREDT
                     VLYPAHGPAIDKPRQLVRGMLGHRRSREAQILRLLDAGPQAIPDFVAAMYKGLDPRLI
                     GAAGRSVLAHLYDLRNRGIVAEEDETWRLST"
     gene            1509..2147
                     /locus_tag="VFV30_11325"
     CDS             1509..2147
                     /locus_tag="VFV30_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX4848727.1"
                     /translation="MAAQHLTAAQRPGRTALAIIAINLAIAAYLVWRAFAPADHGDPI
                     ATSLTAFEKANRLTVFSAQLAPVVSADDVRGMGLLKSRQIAVIPARVDYTLDLARLDA
                     GRFRWDAAGKTLTVTLPPLELSKPNLDEARAQYLREGIWITGPAQAKLTRDNTLEAER
                     QAAQQAANPQLLDLARGSARQAVVQNLAVPLRSAGYAGVTVTVRFDGEPEAP"
     gene            2230..3003
                     /locus_tag="VFV30_11330"
     CDS             2230..3003
                     /locus_tag="VFV30_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848728.1"
                     /translation="MATVTANGAAAQGMDLRFYVKLAWAMALVMVAGFSMQLAMGRSS
                     FSAPPRVHFHAVIFFGWIVLFLGQAHFAAAGNRARHAMLGRVAMSWIVLMLTAGLAVT
                     IALAREGRVPFFFYPQQFVVGDPLSLFTFVGLTAWAVSRRGDPEWHVRLQICALAAIT
                     GPGFGRLLPMPLIAPYGFEAAGLAGLVFPLAGLIRDRRLGLPAHPAWLWGMAAIVAMI
                     AGMDLIAYSPLGDAIYAAMTAGSPGAAVPGLALPPPPPM"
     gene            3088..>3228
                     /gene="nadA"
                     /locus_tag="VFV30_11335"
     CDS             3088..>3228
                     /gene="nadA"
                     /locus_tag="VFV30_11335"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX4848729.1"
                     /translation="MTVQTENLAGIDIRAEIERLRKERNAVILAHYYQKPEIQDLADY
                     IGD"
BASE COUNT          469 a         1172 c         1076 g          511 t
ORIGIN      
        1 cccattcggg acagtcccga tttggtgttg gaaaccgcgt ccctccggct tggcattgcc
       61 gccgcgccgt gcctaagccg aagcaggatg ggaacgcctg ccagcatcaa tccccgcatt
      121 gtcgagggac tctactgcga agccctggtc ctttcggacg aggtgcgtgc ggcctttgcc
      181 ctgtccggac ggctcgacgc cccggccgcc ggtgaccacg gcagcgacga ggcccggatc
      241 gccctgtcgt gcgaggcgct gcgcaccacc acccggatga tgcacgccct cgcctggctg
      301 ctgaaccacc gcgcctactt tgccggagag atctcggaat tccagctgcg ccgccacggc
      361 aagctggcgg aatcgccaga ggccgagccc gagcggctgg ccctgctcga tcccgagatc
      421 cgcagcctga tcctctcgac cgagcgcttt cacgcccggc tcaagcgact cgattccggc
      481 tggcgcgacc gcgaacccgg cgcccccagc gcactggcgg cgctgcgcga aaggctgggc
      541 ttgcaggtcc gcaactagct ttttctcgac agggggaccc gcgctcccca tatcccgctg
      601 gcgatgaaca ttcccccgaa gccctggccc accggcctta gcgaactgtg cgaaccgctg
      661 gtccgccggg tgctggcgcc caacgcctcg cactacacct tcaccggcac gcagacctac
      721 atcgtcggcg ccggggacga ggtggcggtg atcgatcccg gcccggcgga cagcggggac
      781 gaggccgacc cctcgttctt caagggggcg gacaccaacg ggcgcggtca tgtcgaggcg
      841 atcctggccg ccgtgggcgc ggcgcggatc ggcgcgatcc tgtgcaccca cacccaccgc
      901 gaccattccc ccgccgccgc cgtgctgaag gcccgcaccg gagcgccgat catcggctgc
      961 gcgccgctgg tcctggccga cgacgggccg cgcgccgaca gcgccttcga ccagttctat
     1021 tcccccgacc gggttctggc cgatggcgag cgggtcagcg ggccggactg gacgatcgag
     1081 gcggtggcca cccccgggca caccagcaac cacctgtgct atgcgctggt cgaaagcggc
     1141 gcgctgttca ccggggatca tgtcatggcc tggtcgacca gcgtcgtctc gccgccggac
     1201 ggcgatatgg cggcctatat ggccagcctg caaaagctct atgaccgcga ggacacggtg
     1261 ctctatcccg cccacggccc ggcgatcgac aagccgcggc agctggtgcg cggaatgctg
     1321 ggccaccgcc gcagccgcga ggcgcagatc ctgcggctgc tggacgcggg gccccaggcc
     1381 atcccggact tcgtcgcggc gatgtacaag ggcctcgacc cgcggctgat cggcgcggcc
     1441 ggccgttcag tcctggctca cctctacgat cttaggaacc gcggcatcgt ggccgaggaa
     1501 gacgagacat ggcggctcag cacctgactg cggcgcagcg ccccgggcgc accgccctgg
     1561 cgatcatcgc catcaacctg gcgattgccg cctacctggt ctggcgcgcc tttgctccgg
     1621 ccgatcacgg cgatccgatc gccaccagcc tgaccgcctt tgaaaaggcc aaccggctga
     1681 cggtgttttc cgcgcagctc gcccccgtgg tcagcgccga cgacgtgcgc ggcatgggcc
     1741 tgctgaaaag ccgccagatc gcggtgatcc cggcgcgggt cgattacacg cttgatctgg
     1801 cgcggctgga cgccgggcgg ttccggtggg acgcggccgg caagaccctg accgtcaccc
     1861 tgcccccgct cgagctgtcc aagcccaacc ttgacgaggc ccgcgcccag tacctgcgcg
     1921 aagggatctg gatcaccggt ccggcccagg ccaagctgac ccgcgacaac acgctggagg
     1981 ccgaacggca ggcagcgcag caggccgcca acccgcagct gctcgatctg gcccgcggct
     2041 cggcccggca ggccgtggtt cagaacctcg cggtgccgct gcgctcggcc ggatacgcgg
     2101 gcgtgaccgt gacggtccgc tttgacggcg aaccggaagc gccctgagca ctccgccatt
     2161 gccagcccgc gcgatcgggt gcatcttccg atcataaggg gtcgcggaac cgagggggac
     2221 taccgcgata tggcaactgt tactgcaaac ggcgccgcgg cgcagggcat ggacctgcgc
     2281 ttttacgtca agctcgcctg ggccatggcc ctcgtcatgg tcgcgggctt ttcgatgcag
     2341 ctggcgatgg gccgttcctc gttctccgcg ccgccgcggg ttcactttca cgcggtgatc
     2401 ttcttcggct ggatcgtgct gttcctgggc caggcgcact tcgccgccgc cggcaaccgc
     2461 gcccgccacg ccatgctggg gcgggtggcg atgagctgga tcgtgctgat gctgaccgcc
     2521 gggctggccg tgaccattgc ccttgcccgc gaaggccggg tgccgttctt cttctatccg
     2581 cagcagttcg tggtcggcga tccgctttcg ctgttcacct tcgtcgggct gaccgcctgg
     2641 gccgtatcgc ggcgcggcga tcccgaatgg cacgtccggc tgcagatctg cgcgctggcg
     2701 gcgatcaccg ggccgggctt tggccggctg ctgccgatgc cgctgatcgc gccttatggg
     2761 ttcgaggcgg cgggccttgc gggcctcgtc tttccgctgg ccgggctgat ccgcgaccgg
     2821 cggctgggcc ttcccgccca tccggcctgg ctgtggggca tggccgcgat cgtggcgatg
     2881 atcgccggga tggacctgat cgcctattcg ccgctgggcg atgcgatcta tgccgcgatg
     2941 accgccggat cgccgggcgc cgcggtgccg gggctggccc tgcccccgcc cccaccgatg
     3001 taagccggtg cgcctggccg gaaattcggc cttgatcgcg ccccccggaa ggaccatctg
     3061 aggccctctc ccacggagtt gctgaagatg accgtccaga ccgagaacct tgccggaatc
     3121 gacatccgcg ccgagatcga acgcctgcgc aaggagcgca acgcggtgat cctggcgcac
     3181 tattaccaga agccggaaat ccaggacctg gccgactaca tcggcgac
//
LOCUS       DASUDV010000519         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1829260,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000519 DASUDV010000000
VERSION     DASUDV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829260"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFV30_11340"
     CDS             <1..256
                     /locus_tag="VFV30_11340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX4848730.1"
                     /translation="TVEIDHGNGLMTRYAHMSGFRARVGQRVEAGQVIGLIGNTGRST
                     GPHLHFEVRINDRAVNPRPFLEAAANVQQEGGRSAATNGR"
     gene            261..638
                     /locus_tag="VFV30_11345"
     CDS             261..638
                     /locus_tag="VFV30_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4848731.1"
                     /translation="MRGSTFSVLGADMAIKGNVTASTDLHIDGKVEGDVACSSLVQGE
                     TGEIIGAVKAETARLSGIVRGSISARELVILKSARIHGDVHYDALTIEQGAQVDGRFA
                     PRGVGQPEDSPAESVEKLLTLAG"
     gene            complement(651..>2001)
                     /locus_tag="VFV30_11350"
     CDS             complement(651..>2001)
                     /locus_tag="VFV30_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4848732.1"
                     /translation="YTTAELAAQVADSGTRLLVTVDSSALLPTALAVKEQSSLEWLVV
                     GSLASQLPLITGLAMRLLARKSLSPVPPQALRWEDLLVDAAPAPVTLDPEEDLALLQY
                     TGGTTGTPKGAMLTHQNITANTRQVEAIDPRQDERDMIVGVLPMFHVFANICVLNRTV
                     AHGGCIAMLPRFDAGQALKTLARVKATAIPGVPTMYQALLDHPNCGKTDFSSLRVCIS
                     GGAPLPQPLKQRFEATTGARLIEGYGLTETAGVATANPYEGEERPGTIGQPIPGTRIR
                     LLDKEDATQDASAGEPGELAIQGPQVMRGYWRRPEAALAAFAERPDGRWLRTGDVAVL
                     EEGGYLRIVDRIKDMIAVGGFKVFPSEVEAVLLRHPAVKEALVIGVPDPYRGEAPRAY
                     VTLQEDAAETGEALAKWLNAQLGKHERVDQVVVRESLPKTMIGKLDRKALRAEVGMA"
BASE COUNT          340 a          666 c          662 g          333 t
ORIGIN      
        1 cacggttgag attgaccatg gcaacgggct gatgacgcgg tatgctcaca tgtcgggctt
       61 ccgggcgcga gtcggtcagc gggtggaagc cgggcaagtg atcggcctga tcggtaacac
      121 cgggcgctcg acgggtccgc acctgcattt cgaagtccgg atcaacgacc gggcagtcaa
      181 tccccggcca tttcttgagg cggcggcaaa tgttcaacaa gaaggcggcc gcagcgccgc
      241 gaccaacggg cggtaacacc atgcgcggat cgaccttctc ggtccttggg gccgatatgg
      301 cgatcaaggg caatgtcacg gcttcgactg acctgcacat cgacggcaag gttgagggcg
      361 atgtagcctg cagctcgctg gtccagggtg aaaccgggga gatcataggc gcggtcaagg
      421 ccgagaccgc ccggctctca ggcatcgtgc gtggttcgat ttcggcgcgc gaactggtga
      481 tcctcaagtc cgcccgcatc catggcgatg tgcattacga tgcgctgacg atcgaacagg
      541 gcgcgcaggt cgatggccgc tttgctccgc gcggcgtggg ccagcccgaa gacagcccgg
      601 ccgaaagcgt cgaaaagctg ctgacgctcg ccggctaaac cgggcgcacg tcaggccata
      661 cccacctcgg cgcgcagcgc cttgcgatcc agcttgccga tcatcgtctt gggcaggctt
      721 tcccgcacca ccacctgatc aactcgctcg tgcttgccga gttgggcgtt aagccacttg
      781 gccagcgctt cgccagtctc ggccgcgtct tcctgcaggg tgacataggc gcgcggggct
      841 tcaccgcggt aaggatcggg caccccgatc accagcgctt ccttgaccgc cggatggcgg
      901 agcagcacgg cttcgacttc gctcgggaag accttgaaac cgcccaccgc gatcatgtcc
      961 ttgatgcggt cgacaatccg aagatagccg ccctcttcca gcacggcgac atcgccggtg
     1021 cgcagccagc gcccgtcggg ccgctcggca aaggcggcaa gcgctgcttc gggccggcgc
     1081 caatagccgc gcatcacctg tggcccctgg atcgccagtt cgcccggctc tcccgcagac
     1141 gcatcctgcg tcgcatcttc cttgtcgagc aggcgaatgc gcgtgcccgg gatcggctgg
     1201 ccgatcgtgc ccgggcgttc ctcaccctca taggggttgg cggtcgccac cccagcggtc
     1261 tcggtcaggc catagccctc gatcagccgc gcgccggtgg tcgcctcgaa ccgctgcttg
     1321 agcggctgcg gcaacggcgc accgcccgag atgcagaccc gcagcgagct gaaatcggtc
     1381 ttcccgcaat tggggtgatc aagcagcgcc tggtacatgg tcggcacgcc gggaatcgcc
     1441 gtggccttga cccgggccag cgtcttgagc gcctgcccag catcgaaccg cggcagcatg
     1501 gcgatgcagc ccccatgcgc cacggtgcga ttgagcacgc agatattggc gaagacgtgg
     1561 aacatcggca gcacgccgac gatcatgtcg cgctcgtcct ggcgcggatc gatcgcctcg
     1621 acctgacggg tgttggcggt gatgttctgg tgggtcagca tcgccccctt gggggtgccg
     1681 gtggtgccgc cggtatattg cagcagcgcg aggtcttcct cgggatcgag cgtcaccggt
     1741 gcaggtgcgg cgtcgaccag cagatcctcc cagcgcaatg cctgcggcgg gaccgggctt
     1801 agcgacttgc gcgcgagcaa ccgcatggcg agacccgtga taaggggcaa ctggctggcc
     1861 agcgatccga ccacgagcca ttccagcgag gactgctcct tcaccgccag cgcggttggc
     1921 agcagggccg aagaatcgac cgtcaccagc aaccgcgtgc ccgaatccgc cacctgggcc
     1981 gccagttcgg cggtggtgta g
//
LOCUS       DASUDV010000520         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2116241,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000520 DASUDV010000000
VERSION     DASUDV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116241"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VFV30_11355"
     CDS             complement(<1..1719)
                     /locus_tag="VFV30_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX4848733.1"
                     /translation="MASNPAENGLTIPSQAAVTLDHLTDDEIFGEGKKPGFDIRYIVA
                     AVRANLLVIIAIIAAALAIALVATLLDTRRYTAAASVQINDQSQRVLGDAEDTTNQVN
                     NSWDTDRFLQTQVDVLKSRALAMRVMKRLHLEGNARFYEQMEVPAPGPKASPVAVREL
                     TIGLLRGNMQVRLPRTSRIATITFESTDPELSAQVANAFAEEFIQASLQQRYDSSSYA
                     RNFVAGQLQDAKAKLEDSERNLNAYARANGLIRTRDAAPATGSRDGDAGAGASSVTTA
                     SLMQLNAAASQARANRIAAESRLKALNSRPLLADREAMSNPAVQQLFTQRAEIEAKLQ
                     DELTRHLDGHPNVIQLRAQLKTIDAQLQIAAGNVRGSARAEYDAAVATERQLEAQVSA
                     LKSATLAEQDSAVQYNMLAREADTNRALYDGLLQRYKELNATAGISTSNIAIIDRAEA
                     PTGPSSPNLMKNLAVALLAGLGLAAITTFLRNQFDDTVRVPEDVEHKLELPLLGVIPR
                     ARSASPDEELADPKSPVSEGYNSLRGALLYSTSHGLPKSLLVTSSQPAEGKTTTSFAI
                     AQGFARM"
     gene            complement(1734..2432)
                     /locus_tag="VFV30_11360"
     CDS             complement(1734..2432)
                     /locus_tag="VFV30_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX4848734.1"
                     /translation="MSRFWSWLMLAMAGVVLAGCATSSRSDGLPSGQAAYEIAPPAET
                     AERLQAYRIGPGDVVSVQVFQEPELSNDKVQVDDVGRIQLPLAGDILAAGLTAPELAA
                     SIAERLGRNYIRHPRVVVSVVTTRDQTVAVEGEVKMPGVYKVTPQDTLLSAIARAQSP
                     TNVARLDEVIVFRTVNGRRMGARFDLREIRAGRSPDPQILGGDVIVVGFSSLKGVYRD
                     ILQAAPLLNVFTQF"
     gene            complement(2433..3827)
                     /locus_tag="VFV30_11365"
     CDS             complement(2433..3827)
                     /locus_tag="VFV30_11365"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4848735.1"
                     /translation="MGSSGFLRDGHDRAGLCAAGLLAVSLVLGGGGSPAPLPEIAVQL
                     TAALVAAIWLLSGGTASPWQRAPSTAWYLAGLIVLLPALQLIPLPPALWHSLPGRDDK
                     RAALELVGAAQDWRALSLSPARTLASLLAAAAAALCLVLAATLDAAGRRRLIGVAALG
                     GLATLLVGAAQLSGGPGNPLRFFDPEQIYLTGFQANHNSTADVILIAMLAMAAAGRHA
                     LDRGLVRLSPLQLVLTVAAVDAVMVLGLFLTGSRAGLALLPVVLVFQILILRRGDTAQ
                     RAPVRWGRAGLVVGGLLALAAGAAAILRDNRAIGLVLDRFTLAGEFRPQLWADSLFAL
                     GQYWPAGSGQGTFAPVLMAVERLEVVDPTLPNRAHNDYLELAIGGGLPGVILLGIIGL
                     LIGRLAWRALRQPPAGGRAAVLFAQGTLAVIALHSLVDYPLRSMALAALAATAVGLLI
                     PARPEHPAQARGDR"
     gene            complement(3934..4377)
                     /locus_tag="VFV30_11370"
     CDS             complement(3934..4377)
                     /locus_tag="VFV30_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848736.1"
                     /translation="MTSQLFAAAASVALLGAGFASLDGTRSMESLPAVQTITADGVGG
                     AAKKCRVDVVRAGTPGAADIVRDELTDGTCVCIITTGPANNNGAAESIVTNLLRDRTC
                     EGAPPPAGKPAAFVGTPFLIPAIVGTVGAAGAAGLAATAGNDSAG"
BASE COUNT          697 a         1486 c         1576 g          678 t
ORIGIN      
        1 catccgggca aagccctggg cgatggcaaa gctggtggtg gtcttgcctt cggccggctg
       61 cgagctggtg accagcagcg atttcggcag gccgtggctg gtcgaataga gcagcgcccc
      121 gcgcagcgag ttgtagcctt ccgaaaccgg cgacttggga tcggccagtt cctcgtccgg
      181 agaggcgctc cgcgcccgcg ggatcacgcc cagcagcggc agttccagct tgtgctcgac
      241 atcttccggc acgcggaccg tgtcgtcgaa ctggttgcgc aggaaggtgg tgatcgcggc
      301 caggcccagc ccggccagca gggccacggc caggttcttc atcaggttgg gcgaggaagg
      361 gccggtcggc gcttcggccc ggtcgatgat cgcgatgttg ctggtgctga tcccggcggt
      421 ggcgttcagc tccttgtagc gctgcagcag cccgtcatag agggcgcggt tggtgtcggc
      481 ctcgcgcgcc agcatgttgt actgcaccgc gctgtcctgt tcggccagag tggccgattt
      541 cagcgcgctg acctgggctt ccagctggcg ttcggtggcc accgcggcat cgtattcggc
      601 gcgggccgat ccgcgcacgt tgccggcggc gatctgcagc tgcgcgtcga tcgtcttcag
      661 ctgggcgcgc agctggatca cgttggggtg cccgtcgagg tggcgggtca gttcgtcctg
      721 cagcttggcc tcgatctcgg cccgctgggt gaacagctgc tggaccgccg ggttgctcat
      781 cgcctcgcgg tcggccagca gcgggcggga attgagcgcc ttgaggcggc tttcggcggc
      841 gatccggttg gcccgggcct gggatgcggc ggcgttgagc tgcatcaggc tggccgtggt
      901 gacgcttgag gcgccagccc cggcatcgcc atcgcggctg cccgtcgcgg gggcggcatc
      961 gcgggtccgg atcaggccgt tggcgcgggc ataggcgttg aggttgcgct ccgaatcctc
     1021 cagcttggcc ttcgcgtcct gcagctggcc cgccacgaag ttgcgggcat aggacgagct
     1081 gtcatagcgc tgctggagcg aggcctggat gaactcctcg gcaaaggcat tggccacctg
     1141 ggccgacagt tccggatcgg tgctttcaaa ggtgatcgtg gcgatccgcg acgtgcgcgg
     1201 caggcggacc tgcatgttgc cgcgcagcag cccgatggtc agctcgcgca ccgccactgg
     1261 cgaggccttg ggcccgggtg ccggcacttc catctgctca tagaagcgcg cgttgccttc
     1321 caggtgcagc cgcttcatca cccgcatcgc cagggcgcgg cttttcagca cgtcgacctg
     1381 ggtctgcagg aagcggtcgg tatcccagga gttgttgacc tggttggtgg tgtcttcggc
     1441 atcgcccagc acgcgctgcg actggtcgtt gatctggacc gaggcggcgg cggtatagcg
     1501 gcgggtatcg agcagggtcg ccaccagcgc aatcgccagc gccgccgcga tgatcgcgat
     1561 gatcaccagc aggttggccc gcaccgccgc gacgatgtag cggatgtcga agcccggctt
     1621 cttgccttcg ccgaaaatct cgtcgtcggt caggtggtcc agcgtcaccg ctgcctgcga
     1681 tggaatggtc aggccgttct cggcgggatt cgatgccatc ggtcggcgaa ccttcagaac
     1741 tgggtaaaga cgttgagcag cggcgctgcc tggaggatgt cgcggtagac gcccttgagc
     1801 gaggagaagc cgaccacgat cacgtcgccg cccaggatct gcggatcggg cgagcggccg
     1861 gcgcggatct cgcgcaggtc aaaccgcgcg cccatccgcc ggccgttgac cgtgcggaac
     1921 acgatcactt cgtccagccg ggcgacattg gtcgggcttt gcgcccgggc gatggcggac
     1981 agcagcgtgt cctgcggggt caccttgtag accccgggca tcttcacttc gccttccacc
     2041 gccacggtct gatcgcgggt cgtaaccacg ctgacgacga cgcggggatg gcggatatag
     2101 ttgcggccca gccgctcggc tatcgaggcg gcgagttccg gggcggtcag cccggcggcc
     2161 aggatgtccc cggccagcgg cagctggatg cggccgacat cgtccacctg gaccttgtcg
     2221 ttcgacagtt cgggttcctg gaagacctgc acgctgacca cgtcgccggg gccgatgcgg
     2281 taggcctgca gacgctccgc cgtttcggcc ggcggggcga tctcgtacgc ggcctggccg
     2341 gagggcaagc cgtccgaccg gctcgacgtg gcacagcccg ccaggaccac gcctgccatt
     2401 gccagcatca gccacgacca gaacctgctc acttacctgt ccccccgggc ttgcgctgga
     2461 tgctccggcc tggcgggaat cagcaggcca acggccgttg cggccagcgc cgccaatgcc
     2521 atggaacgca gcgggtaatc aaccagcgag tggagcgcga tcaccgccaa tgtgccctgc
     2581 gcgaagagca ccgccgcgcg gcccccggcc ggcggctggc gcagcgcccg ccaggccagc
     2641 cggccgatca gcaagccgat gattcccaac aggataacgc ccggcagacc gccgccgatc
     2701 gccagctcca gatagtcgtt gtgggcccgg ttcggcaggg tcggatcgac cacttcgagc
     2761 cgttcgaccg ccatcagcac cggggcaaag gtgccctggc ccgatccggc cggccagtat
     2821 tggcccaggg cgaagagcga atccgcccag agctgcgggc ggaattcccc ggcgagggtg
     2881 aaccggtcga gcacgaggcc gatcgcgcgg ttgtcgcgca ggatggccgc ggcgccggcg
     2941 gccagggcca gaaggccgcc caccaccagt ccggcccggc cccagcggac cggtgcgcgc
     3001 tgcgccgtat cgccgcgccg caggatcagg atctggaaga ccagaaccac cggcagcaag
     3061 gccaggccgg cccgcgatcc ggtcaggaac aggcccagta ccatgacggc gtccaccgcg
     3121 gcgacggtca ggaccagttg cagcgggctg agccgcacca gcccgcgatc aagcgcgtgc
     3181 cgcccggctg cggccatggc cagcatggcg atcaggatca cgtcggcagt gctgttgtgg
     3241 tttgcctgaa acccggtgag gtagatctgt tcgggatcga agaaccgcag cggattgccg
     3301 gggccgcccg aaagctgggc cgcgccgacc agcagcgttg ccaatccgcc cagcgcggca
     3361 acgccgatca gccggcggcg cccggccgca tcgagcgtgg cggccagcac caggcacagc
     3421 gcggcagcgg cggcggcgag caggctggcc agggtccgtg cgggcgagag cgacagcgcc
     3481 cgccaatcct gggccgcacc gaccagttca agcgcggcgc gcttatcgtc ccggccgggc
     3541 aggctgtgcc acagggcggg gggcaagggg atcagttgca gggcgggcag cagcacgatc
     3601 agcccggcca gataccaggc ggtggacggt gcgcgctgcc agggtgatgc cgtgccgccg
     3661 ctgagcagcc agatcgcggc gaccagcgcc gcggtcaact ggacggcgat ttccggcagc
     3721 ggcgcgggcg accctccgcc gcccagcacc agcgagacgg ccagcagtcc ggcggcgcac
     3781 agcccggcgc gatcgtgccc gtcccgcaag aagccgctgc tgcccaacgc cctgccccca
     3841 ccctgttcgg ccgcgccaag gcgcgcaaaa cctcaggccg aagcgaacca cttcgaccgc
     3901 taccgagtca attcggaaat ggaattcggg gcttcagccg gctgaatcgt tgcccgcagt
     3961 ggcggccagg ccagccgcgc cggccgcgcc caccgtgccg acgatagctg gaatcaggaa
     4021 aggcgtccct acaaaagcag ctggcttgcc agccggggga ggagcgcctt cgcaagtgcg
     4081 gtcgcgcagc aggttggtga cgatcgattc cgccgcgccg ttgttgttgg cagggccagt
     4141 ggtgatgatg cagacgcagg tgccgtcggt cagttcgtcg cgaacgatgt cggccgcgcc
     4201 cggggttccg gcgcgcacaa cgtcaacccg gcacttcttg gccgcaccgc ccacgccatc
     4261 ggcggtgatg gtctggacag cgggcaggct ttccatcgaa cgggtgccat caagcgacgc
     4321 gaaaccggcc cctagcaggg caaccgatgc ggcggcggcg aacagttgcg aagtcacgaa
     4381 tgcaggctcc gggtaaattt ctctagggga attaccatag caatcgttag caagtct
//
LOCUS       DASUDV010000521         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_702611,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000521 DASUDV010000000
VERSION     DASUDV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702611"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1235
                     /gene="recF"
                     /locus_tag="VFV30_11375"
     CDS             162..1235
                     /gene="recF"
                     /locus_tag="VFV30_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX4848737.1"
                     /translation="MTLERITLTSFRNHRATRLDGTARFNLLVGENGAGKTNVLEAIS
                     LLSPGRGLRRAALPELAMHQADAGFAVSAELAGSVQLGTGARPDRPNRRIAQVNGAEV
                     AAVQLGEWLALGWLTPAMDRLFAESAGARRRFLDRLVLALEPGHARNAARLEAALRER
                     NRLLSEQAEPDPTWLDAIEAQLVEAGTLVAAARARLIERLRASLDALPDTPFAKPLLA
                     YQPGGPLDPFELTAQLAAGRRRDRAAQRTLTGPHRDELVVTMAGKNQPAAECSTGEQK
                     AMLIAITLAHAALLDPARPGVLLLDEVAAHLDPLRREALFDRLRTGRAQVWLTGTELP
                     PFEAIVREAALWRVSEGSVQRLD"
     gene            complement(1204..1908)
                     /locus_tag="VFV30_11380"
     CDS             complement(1204..1908)
                     /locus_tag="VFV30_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX4848738.1"
                     /translation="MKRIWASAMGLVLLAGCQSEPASPSSEQPAAAAAPAPRGEEIPI
                     LALGDSLFAGYGLEPGQSYPSRLEAELQRRGINARIANAGVSGDTTAGGLQRLDFALS
                     GQARPPVLVIISLGGNDMLRGLSPAETRANLDAILTRLGERKIKALLLGMLAAPNLGA
                     DYRGKFDPIYPQLAKKHDAALVPFFLQPLIDKPQLIQADRIHPTLEGIDLLVADTVEE
                     VEGALPVSPGAEQNPR"
BASE COUNT          321 a          728 c          667 g          356 t
ORIGIN      
        1 ggagccgtgc caatttcgaa aatcggcaga aatccaagct ttccgaatcc tgaaccgatt
       61 ccctcgcaga ggtgaatgct cacagttagt attttttacc aacttttggt atccgagttt
      121 cgcgtgactt tgctgcccgc cccgctctat cgcgttcggc gatgacgctc gagcggatca
      181 ccctcacctc gtttcgcaac catcgcgcca cccggctcga tggcacggcc cgcttcaacc
      241 tgctggtggg cgaaaacggg gctggcaaga ccaacgtgct cgaagcgatt tcgctgcttt
      301 caccgggccg gggcctgcgc cgtgcggccc tgcccgaact ggcgatgcac caggccgacg
      361 ccggttttgc ggtgagtgcc gaactggccg gatcggtgca gctgggcacc ggcgcgcggc
      421 ccgatcggcc gaaccggcgg atcgcccagg tcaacggagc cgaagtggca gccgtgcagt
      481 tgggcgaatg gctggcgctg ggctggttga ccccggcgat ggaccggctg tttgccgaga
      541 gcgcgggcgc gcggcggcgc tttcttgatc ggctggtgct ggcgctcgaa ccgggccatg
      601 cccgcaatgc cgcgcggctc gaggccgcgc tgcgcgagcg caaccgcctg ctgagcgagc
      661 aggccgagcc cgatccgacc tggcttgatg cgatcgaggc ccagctggtt gaggccggta
      721 ccctggtcgc cgcggcccgg gcccggttga tcgagcgcct gcgcgcctcg ctcgacgccc
      781 tgcccgacac gccctttgcc aagccgttgc tggcctacca gcccggcggc ccgctcgacc
      841 cgttcgaact gactgcccag ctcgccgccg ggcgtcgccg tgatcgcgcc gcccagcgca
      901 ccctgaccgg cccgcaccgc gacgaactgg tcgtgaccat ggccggcaag aaccagcccg
      961 ccgccgaatg ttcgaccggc gaacagaagg ccatgctgat cgccatcacc ctggcccatg
     1021 ccgctctgct cgatccggcg cgcccgggcg tgctgttgct tgacgaggtg gcagcgcacc
     1081 tcgatccgct gcgccgcgaa gcgctgttcg accggctgcg caccggccgc gcacaggtgt
     1141 ggctgaccgg aaccgagctg ccccccttcg aggcgattgt ccgggaagcc gcgctgtggc
     1201 gggtcagcga gggttctgtt cagcgcctgg actgaccggc agcgcgcctt ccacttcttc
     1261 aaccgtgtct gccaccagca gatcgattcc ctccagcgtc gggtggatcc ggtcggcctg
     1321 gatcagctgc ggcttgtcga tcagcggctg caggaagaac gggaccagcg ccgcatcatg
     1381 cttcttcgca agctgcggat agatcggatc gaacttgccg cgatagtccg cgccaaggtt
     1441 gggcgcagcc agcatgccca gaagcagcgc cttgatcttg cgttcgccca gcctggtcag
     1501 gattgcatcg agattggccc gggtttcggc cggcgaaagg ccgcgcagca tatcgttgcc
     1561 gcccaggctg atgatcacca gcaccggagg ccttgcctgg cccgacagcg caaaatcgag
     1621 ccgctgcagg ccgccggcag tggtatctcc cgaaacccct gcgttggcga tccgggcgtt
     1681 gatcccgcgt cgctgcaatt cggcttcgag ccgcgaggga tagctttgcc ccggctcaag
     1741 cccgtatccg gcaaacaggc tgtcgcccag cgcaaggatc ggtatctcct cccccctggg
     1801 tgctggcgcg gcggcggcgg ccggctgttc ggacgagggc gatgccggtt cggactggca
     1861 tccggccagc agcaccaagc ccatcgccga agcccatata cgtttcatcg agcaacctcc
     1921 ttgcctttgt ccctagctat ggcaaaccgc gcgcgtgaca agtccccatc tggcaatcgc
     1981 cgcttcgaac cttaccctca cccttggcca gggcagcgcc gcagtagaga tcctgcgcgg
     2041 gatcgacctt gcggtgcccc tgggccagac cc
//
LOCUS       DASUDV010000522         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1188470,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000522 DASUDV010000000
VERSION     DASUDV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188470"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFV30_11385"
     CDS             complement(<1..265)
                     /locus_tag="VFV30_11385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX4848739.1"
                     /translation="MTSHPAPPEGLIAGRQHRFAVRAYYEDTDLSGVVYHANYLRWFE
                     RARSDFVRLIGIDQRAAVESGEGAFAVADMAIRYAAPARLDDAV"
     gene            complement(354..731)
                     /locus_tag="VFV30_11390"
     CDS             complement(354..731)
                     /locus_tag="VFV30_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX4848740.1"
                     /translation="MPIDATQPYDDQNIFARILRGEIPNRTVHEDEWTLAFHDINPQA
                     PIHVLVIPKGAYVSWDDFSARAGAEEIAGFVRAVGHVARELGLVEPGYRLLANIGGHG
                     HQEVPHLHVHLFGGRPLGPMLAR"
     gene            complement(731..1045)
                     /locus_tag="VFV30_11395"
     CDS             complement(731..1045)
                     /locus_tag="VFV30_11395"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX4848741.1"
                     /translation="MDTLTRLAATIAQRRGADPETSWVAKLNARGVPVIARKLGEEAV
                     ETVIAALAGSREELVGEAADVLFHLLVLLDAKGVALDEVLAELDRREGTSGIAEKASR
                     SQ"
     gene            complement(1353..1538)
                     /locus_tag="VFV30_11400"
     CDS             complement(1353..1538)
                     /locus_tag="VFV30_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848742.1"
                     /translation="MTRVARIALILTGLGLAAAFSTPGLAAAAAVIPEPTDMTLIAMA
                     FAGLIAGRYIARGRKSD"
     gene            complement(1624..2385)
                     /gene="hisF"
                     /locus_tag="VFV30_11405"
     CDS             complement(1624..2385)
                     /gene="hisF"
                     /locus_tag="VFV30_11405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX4848743.1"
                     /translation="MTVRIRVIPCLDVRDGRVVKGVNFVDLRDAGDPVEQARAYDAAG
                     ADELCFLDISASHEGRGTLLDVVRRTAEVCFMPLTVGGGVRRAEDARALLLAGADKVA
                     VNSAAVSRPEVVSEIADRFGSQCVVASVDARRRAAGGWEIFTHGGRKATGIDAVEHAL
                     RLAELGAGELLVTSMDGDGTQAGYDLDLTRTIADAVSVPVIASGGVGTLDHLVEGVTR
                     GHASAVLAASIFHFGTHTIAQAHAALRAAGLPARG"
     gene            complement(2382..>2775)
                     /locus_tag="VFV30_11410"
     CDS             complement(2382..>2775)
                     /locus_tag="VFV30_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX4848744.1"
                     /translation="VKDMARDWPGGIVVAVDAKDGMVATEGWAAVSDVPVVDMARRFE
                     DAGVASLLFTDIGRDGLLKGVNIDATLDLARRVDIPVIASGGVKGLDDIHVLALHAHE
                     GIEGVITGRALYDGRLDLAAAIAMANRA"
BASE COUNT          467 a          973 c          892 g          443 t
ORIGIN      
        1 gcacggcatc gtcaagccgc gccggggcgg cataacggat cgccatgtcg gccacggcaa
       61 aggcgccttc gccgctttcc accgcggcgc gctgatcgat cccgatcagc cgcacgaaat
      121 cgctgcgcgc gcgttcgaac cagcgcaggt aattggcgtg ataaaccacc cctgaaaggt
      181 cggtgtcctc gtaataggcg cgcaccgcaa agcggtgctg gcggcctgcg atcaggcctt
      241 cgggcggagc cgggtgcgac gtcattgcgc caagccctta gcaagtgcgc gactcgcagg
      301 gcaagcagct tgtcgaggac tgtgcttcag ctgcgccgaa gcgttggccc tgatcagcgg
      361 gccagcatcg gtcccagcgg cctgccgcca aacaggtgga cgtgcaggtg cggcacttcc
      421 tggtggccat ggccaccaat gttggcgagc agacggtagc ccggctcgac cagccccagc
      481 tcgcgcgcga catggccgac cgcgcgcacg aagccggcga tctcctctgc cccggcgcgg
      541 gccgaaaaat catcccagct gacataggcc cccttgggga tcaccagcac gtggatcggc
      601 gcctgcgggt tgatatcgtg gaaggccagc gtccattcgt cttcatggac ggtccggttc
      661 gggatttcgc cgcgcaggat cctggcgaaa atgttctgat cgtcataggg ctgagtggcg
      721 tcgatcggca ttactggctc cggctggctt tttcggcgat cccgctggtc ccttcgcggc
      781 ggtcaagctc ggccaggacc tcgtccagcg caacgccctt ggcgtcgagc aggaccagca
      841 ggtggaacag gacgtcggcc gcttcaccga ccagctcctc gcggctgccc gcaagcgcgg
      901 cgatcacggt ttcgaccgct tcctcgccca gcttcctggc gatcaccggc accccgcgcg
      961 cgttgagctt tgccacccag ctggtttcgg gatcggcacc gcggcgctgg gcgatggtgg
     1021 cggcaagacg ggtcagcgtg tccatcgcgc tggaatgggg cgtggccgcg cgccggtcaa
     1081 ggtcaatacc aagctccact gatcccgacc catgaggatt gcgctacccc gtttgctggc
     1141 gaggagcagc cgcagcggcg atgtggttca tcatcgcaag gttgcgacga agtccagcga
     1201 gcggggtagc gcaacccttc gggcggcctt ctggtggccc tcgggctgcg tcgcggcttc
     1261 ggcgtgatga accaacatga ccccttcaga cgctccttgc cgagaaccgc cagaagaccg
     1321 tcctcatggg tcgggatcag tggagcttgg tatcagtcgc tcttgcggcc cctggcgatg
     1381 tagcgtccgg cgatcagccc ggcgaaagcc atggcaatca gtgtcatgtc ggtcggttcg
     1441 ggaatgaccg ccgcagccgc ggcaaggccg ggcgtgctga atgcggcagc caggcccagc
     1501 ccggtaagaa tcaatgcgat gcgcgccacc ctggtcatgg tccggccttg ggttgcatcg
     1561 tgttgcaaag caaacgattt tccgggagcg tcccagcttg ggactgaccc cgcaatcagc
     1621 ctttcagccc cgcgcgggca gccccgccgc gcgcagtgcc gcatgggcct gggcgatggt
     1681 atgggtaccg aagtggaaga tcgaggcggc caggaccgcg ctggcatggc ccctggtcac
     1741 cccttcgacc agatggtcga gcgtgcccac cccgccgctc gcgatcaccg gcaccgaaac
     1801 cgcatcggca atggtgcggg tcaggtcgag atcataacca gcctgggtgc catcgccatc
     1861 catcgaggtg acgagcagtt cgcccgcgcc cagttcggcc agccgcagcg cgtgttcgac
     1921 cgcatcgatt ccggtggcct tgcgcccgcc gtgagtgaag atttcccagc cgccagccgc
     1981 gcgccgccgc gcatcgaccg aggcaaccac gcactggctg ccgaagcgat cggcaatttc
     2041 gctgaccacc tcggggcgcg aaaccgcagc ggaattgacc gcaaccttgt cggccccggc
     2101 cagcagcagc gcgcgcgcat cctcggcgcg gcgcaccccg ccgcccacgg tcagcggcat
     2161 gaagcagacc tcggcggtgc gccgcaccac atcgagcagg gtcccgcgcc cttcgtggct
     2221 ggcgctgata tcgagaaagc acagctcgtc cgccccggcc gcatcatagg cccgcgcctg
     2281 ttcgaccgga tcgccggcat cgcgcaggtc cacgaagttg acccccttga ccacgcgccc
     2341 atcgcgcaca tcgagacaag ggatgacgcg gatgcggacg gtcatgcccg atttgccatg
     2401 gcaattgcgg cggccagatc gagccgccca tcgtacagcg cacgccccgt aatcacgccc
     2461 tcgatccctt cgtgggcgtg aagcgccagc acgtggatat cgtcgagccc cttgaccccg
     2521 cccgatgcga tcactggaat gtccacccgc cgcgccagat cgagcgtggc atcgatattg
     2581 acgcctttca gcagcccgtc gcggccgata tcggtgaaca gcaggctggc caccccggca
     2641 tcctcgaagc ggcgggccat gtcgaccacc ggcacgtcgg aaaccgccgc ccagccctcg
     2701 gtcgcgacca tgccgtcctt cgcgtcgacc gccaccacga tcccgcccgg ccagtcgcgc
     2761 gccatgtcct tgacg
//
LOCUS       DASUDV010000523         6077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2072597,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000523 DASUDV010000000
VERSION     DASUDV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072597"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFV30_11415"
     CDS             complement(<1..416)
                     /locus_tag="VFV30_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4848745.1"
                     /translation="MPISKQQHLAGAFAALALFALPQAAQANAAAMDYFTKRIDRSAV
                     PTLLSAEDRTYYRNLFAAIDRGDWARVQALLAERPDGPLHQVARAEYYLAAGSPKIEL
                     EQLNAWLAGGVSLPQAPEIAALAQRRGATSVPALPAA"
     gene            complement(453..1220)
                     /locus_tag="VFV30_11420"
     CDS             complement(453..1220)
                     /locus_tag="VFV30_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848746.1"
                     /translation="MTFSAANSRSASPLAGDLAAALDWWREAGLEHDFRATAADWLAP
                     RAPAVTEAQPIAAQAPPPVIEAPRPEPIGGGSENWPQDLPGFIGWWMAEPSLDFGQVQ
                     ARVPPRGAQGAELLIVVDHPEAGDSDRLLSGEQGRLAEAILAALGVAPEKAYFAAALV
                     RHTPLPDWPALADAGLGAILAHHIRLAAPRRVISFGPHVSSLLGHDPAKSAEPLRQFY
                     HVGGQIPALAAPGLTTLAARPKGKAALWRALLEWQHG"
     gene            1303..2952
                     /locus_tag="VFV30_11425"
     CDS             1303..2952
                     /locus_tag="VFV30_11425"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX4848747.1"
                     /translation="MSERESMPYDVVIVGGGPAGLAASIRLKQVNPDLSVCILEKGSE
                     VGAHILSGAVVDPKALDELLPDWRTQGCPMAETPVTDNWHWVLSKGKKYSIPHWPMPP
                     FMSNDGNYTGSLGNLCRWLAGKAEELGVEIFPGFPASEVLYNEDGSVKGVATQDMGIA
                     KDGSQKGDYQPGMELHAKYTFFAEGARGHLTKQLKGKFDLEANCEPQVYGIGIKELWD
                     IDPEKHVPGRVIHTQGWPLSESDSWGGGFLYHQANNQVALGFVTALDYKNPYVYPFEE
                     FQRWKQHPEIRAILEGGRRVSYGARAINEGGWQSVPKLDFPGGALIGCSAGFVNVPRI
                     KGSHTAMKSGMLAAEAAAAAIAAGREHDSMAEYDAAVRDSWIATELKKVQNAEPMVAK
                     WGGDLGTILAGADMWLRTLFGFGLWKPMKHHRDCEAIQRADLYRPIDYPKPDGVISFD
                     RLSSVALSFTNHEEDQPCHLQLKDPAIPVTVNLAHYAGPEARYCPAGVYEFLGIEDGN
                     PRLQINAQNCVHCKTCDIKDMTQNINWVTPEGGGGPNYPNM"
     gene            3014..3448
                     /locus_tag="VFV30_11430"
     CDS             3014..3448
                     /locus_tag="VFV30_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX4848748.1"
                     /translation="MINGPREVVKRARKLRSEMSLPEGLLWRELRKRPGGFKFRRQHP
                     AGTYVLDFYCPAVRLAIEVDGFAHDSIRAARADATRSHFLRSQHIATTRIPATAILED
                     LSAVVVRLIEICDTRVRRSSEELPVPLHHPADGSPPRAGEEL"
     gene            3445..4251
                     /locus_tag="VFV30_11435"
     CDS             3445..4251
                     /locus_tag="VFV30_11435"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX4848749.1"
                     /translation="MIETAYAKINLALHVRRRREDGYHELETLFAFVDAGDVLSIAPA
                     ERDSLRTVGAFAGALTDPFDNIVMKALSALPRPDGWAVTLEKNLPVSAGLGGGSADAG
                     ALFRLYERIYALPRDWHAKATRLGADVPACVISETCVGRGTGTELETVENDLSGTPVL
                     LVNPRMAVSTADVFRRWHGVDEGPLPEGTAREIALAGRNDLLLAANGLCPVIQTILNV
                     LNDNWAWLARMSGSGATCFALFDSDAARDAASARIAADYPGWWQLAGRLR"
     gene            4248..4976
                     /locus_tag="VFV30_11440"
     CDS             4248..4976
                     /locus_tag="VFV30_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX4848750.1"
                     /translation="MRQHEAFCLIGTPRFGGILVVSDHASGRVPEDIDLGIDPALLSQ
                     HIAIDIGVAEVGAAMAERPGIAAFQGNISRLVCDLNRDPHAPAVIPIASDGHAIPGNA
                     VDHAAHLARLARFHDPYHAALAELLNTAPPALILSLHSFTPQLASSDEPRPWQVGVLY
                     NEDDRAARIAIPLLEADGLVVGDQQPYSGKLLNYTMDRHAEADGRPYLGIEIRQDQIA
                     DPAGQAEWAERLARIANRVALELG"
     gene            5051..5581
                     /locus_tag="VFV30_11445"
     CDS             5051..5581
                     /locus_tag="VFV30_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HEX4848751.1"
                     /translation="MSVPSLHPDEARRLIEQGKAVLIDIRSPQEHARERIPGAVCIPL
                     DQLQQAAGPVPEGPVVFHCRSGMRTDANRAVLAQASAGQPCYVLEGGIEGWKRHGHST
                     LFDRSQPIDIMRQVQIAAGTLVLIGVLGGVFVTPLLYGLAGFVGAGLTFAGLSGFCGM
                     ARLLALMPWNRAAQGA"
     gene            5683..>6077
                     /locus_tag="VFV30_11450"
     CDS             5683..>6077
                     /locus_tag="VFV30_11450"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4848752.1"
                     /translation="MPVYRSRTSTHGRNMAGARGLWRATGMKDGDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQMVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMA"
BASE COUNT         1029 a         1971 c         2084 g          993 t
ORIGIN      
        1 gcggcgggca gcgcgggcac cgaggtggcc ccgcggcgct gggccagggc cgcgatctcc
       61 ggcgcctggg gcaggctgac tccgcccgcc agccacgcgt tcagctgctc cagctcgatc
      121 ttcggcgatc ccgcggccag atagtattcg gcccgcgcca cctggtgcag cgggccatcc
      181 gggcgctcgg ccagcagggc ctggacccgc gcccagtccc cccggtcaat cgccgcgaag
      241 aggttgcggt aataggtccg gtcctcggcc gacagcaggg tcggcaccgc gctgcggtcg
      301 atccgcttgg tgaaatagtc catcgccgcg gcgttggcct gggcggcttg gggaagtgcg
      361 aacagagcca gcgcggcaaa agcgccggcc aggtgttgct gcttggaaat tggcacgtgc
      421 tctatggtcc ccgcgttgtc ccgccccggc aatcagccgt gctgccattc aagcaaggcg
      481 cgccacaggg ccgccttgcc cttgggccgc gccgccaggg tggtcagccc cggagcggcc
      541 agagccggga tttggccgcc tacatggtag aattggcgca aaggttcagc gcttttcgcc
      601 gggtcgtggc caagaagcga cgagacgtgc gggccgaagc tgatcacccg gcgcggagcc
      661 gccaggcgga tgtggtgggc caggatcgcg cccaggcccg catcggccag cgccggccaa
      721 tcgggcagcg gcgtatggcg gaccagcgcg gcggcgaaat aggccttttc cggcgcaacg
      781 cccagcgcgg ccaggatcgc ctcggccagc cggccctgtt cgccggacag caggcggtcc
      841 gaatcccccg cttccggatg gtcgaccacg atcagcaact cggccccttg cgcgccgcgc
      901 ggcggcaccc gggcctgaac ctgtccgaaa tcgagcgaag gttcggccat ccaccagccg
      961 atgaagccgg gcaggtcctg cggccagttt tcgctgccgc cgccgatcgg ttcggggcgg
     1021 ggggcttcga tcaccggcgg cggtgcctga gcggcgatcg gctgcgcttc cgtaacggca
     1081 ggcgccctgg gggccagcca atcggcggcg gtagcgcgga aatcgtgctc cagcccggcc
     1141 tcgcgccacc aatccagcgc ggccgcaaga tcaccggcaa gcggggacgc ggaacgggaa
     1201 ttggcggcgg agaaagtcac gcgtcgggtc ttgacctgcc cagcgccagc tttcaagggt
     1261 tggcgcgcag aaaagccaac aacagggtgt ggaaccaggt ccatgagcga acgcgaatcg
     1321 atgccgtatg acgtggtgat cgttggaggc gggcccgctg gcctcgccgc ttcgatccgg
     1381 ctgaaacagg tcaatcccga cctttcggtc tgcatcctgg aaaagggcag cgaagtgggc
     1441 gcccacatcc tgtcgggcgc ggtggtcgat cccaaggcgc tggatgaact gctgcccgac
     1501 tggcggacac agggctgccc gatggcggaa accccggtga ccgacaactg gcactgggtg
     1561 ctcagcaagg ggaagaagta ttcgatcccg cactggccga tgccgccgtt catgtcgaac
     1621 gacgggaact acaccggcag ccttggcaac ctgtgccgct ggctggccgg caaggccgaa
     1681 gagctgggcg tggaaatctt ccccggcttc ccggccagcg aagtgcttta taacgaggac
     1741 ggctcggtca agggcgtggc gacgcaggac atgggcattg ccaaggacgg cagccagaag
     1801 ggcgattacc agcccggcat ggagctccac gccaagtaca ccttctttgc cgaaggtgcg
     1861 cgcggccatc tgaccaagca gctcaagggc aagttcgatc tggaagcgaa ctgcgaaccg
     1921 caggtttacg gcatcggcat caaggagctg tgggacatcg atcccgaaaa gcacgtgccg
     1981 ggccgggtga tccacaccca gggctggccg ctttcggaaa gcgacagctg gggcggcggg
     2041 ttcctgtacc accaggccaa caaccaggtc gcgctcggct tcgttaccgc gctcgattac
     2101 aagaacccct acgtctatcc gttcgaggaa ttccagcgct ggaagcagca cccggaaatc
     2161 cgcgcgatcc tggaaggcgg ccgccgcgtg tcctatggcg cgcgggcgat caacgaaggc
     2221 ggctggcagt cggtgcccaa gctcgatttc ccgggcggcg cgctgatcgg ttgctcggcc
     2281 ggcttcgtca acgtgccccg gatcaagggc agccacaccg cgatgaagag cgggatgctg
     2341 gccgccgaag ccgccgctgc cgcgatcgcc gccgggcgtg agcacgattc gatggccgaa
     2401 tacgatgccg ccgtacgcga cagctggatc gcgaccgaac tgaagaaggt tcagaacgcc
     2461 gaaccgatgg tcgccaagtg gggcggggac ctcggcacga tcctggccgg tgcggacatg
     2521 tggttgcgca cgctgttcgg ctttggcctg tggaagccga tgaagcatca ccgcgattgc
     2581 gaagcgatcc agcgggccga tctctatcgg ccgatcgact atcccaagcc cgacggcgtg
     2641 atcagctttg atcggctgtc cagcgtggcg ctgagcttca ccaaccacga ggaagatcag
     2701 ccctgccacc tgcagctgaa ggatccggcg atcccggtga cggtcaacct cgcgcactat
     2761 gccgggcccg aggcccgcta ttgcccggcc ggggtctatg aattcctcgg catcgaggac
     2821 ggcaacccgc gcctgcagat caacgcccag aactgcgtcc actgcaagac ctgcgacatc
     2881 aaggacatga cgcagaacat caactgggtg acgccagaag gcggcggcgg gccgaactat
     2941 ccgaatatgt gaggcatgat cttcctcccc ttcaggggag ggggaccagc gaagctggtg
     3001 gaggggcacg cgcgtgatca acggacctcg cgaggtcgtg aaacgggccc gcaagcttcg
     3061 tagcgagatg tcgcttccgg aggggctgct ttggcgcgag ctgcgcaagc ggccgggtgg
     3121 tttcaagttc cggcgacagc atccggccgg aacatacgtg ctggactttt actgcccggc
     3181 ggtgaggttg gcgattgaag ttgatggttt tgcgcatgac tccatccgcg ccgccagagc
     3241 cgatgcgacg cgttcgcatt ttcttcgatc gcaacatatc gcgactacga gaattccggc
     3301 cacggccatt ttggaggatt tgagcgcggt ggtcgtgcgg ttgattgaaa tttgcgacac
     3361 gcgtgtacgg cgaagttcgg aagaactgcc cgtgcccctc caccatcctg cggatggttc
     3421 ccctccccgt gccggggagg aattatgatc gaaaccgcct acgccaagat caacctcgcg
     3481 ctgcacgttc ggcggcggcg cgaggatggc tatcacgagc tggaaacgtt gttcgccttc
     3541 gtcgatgccg gcgatgtgct gagcattgca ccggccgagc gggacagcct gcgcacggtc
     3601 ggcgcgtttg ccggggccct gaccgatccc ttcgacaaca tcgtgatgaa agcgctctct
     3661 gccctgccgc ggccggatgg ctgggccgtg acgctggaaa agaacctgcc ggtgtccgcc
     3721 gggcttggcg gcggatcggc cgatgccggg gcgctgttcc ggctctacga gcggatctat
     3781 gccctgccgc gggactggca cgcgaaggcg acccggctcg gtgccgacgt tccggcctgc
     3841 gtgatcagcg aaacctgtgt cgggcgcggc accgggaccg agctggaaac cgtcgagaac
     3901 gatctgagcg gcaccccggt gctgctggtc aatccgcgga tggcggtgtc gaccgccgat
     3961 gtctttcgcc gctggcacgg ggtggacgaa gggccgctgc cggaagggac cgcgcgcgaa
     4021 atcgcgctgg ccgggcggaa cgatctgctg ctggccgcga acgggctgtg cccggttatc
     4081 cagacgatcc tcaacgtgct gaacgacaac tgggcctggc tggcgcggat gtcgggttcc
     4141 ggggcgacct gctttgccct gttcgacagc gatgccgcgc gcgatgcggc ttcggcccgg
     4201 atcgcggcgg actatccggg ctggtggcaa ctggctggca ggctgcgttg agacagcacg
     4261 aggccttttg cctgatcggc acgccgcgct ttggcgggat cctggtcgtg tcggatcacg
     4321 cctcgggccg tgtgccggag gatatcgacc ttggcatcga tccggcgctg ctgagccagc
     4381 acatcgcgat cgatatcggc gtggccgaag ttggcgcggc catggccgag cgtcccggga
     4441 tcgcggcctt tcagggcaac atcagccgcc tggtctgcga tctcaaccgc gatccccatg
     4501 cgccggccgt cattccgatc gccagcgacg ggcacgcaat ccccggcaac gcggtggatc
     4561 acgccgcgca cctggcgcgc ctggcccggt ttcacgatcc ctaccatgcg gccctggccg
     4621 agctgctcaa caccgcgccg cccgcgctga tcctgtcgct gcacagcttc accccgcagc
     4681 ttgccagcag cgacgagccg cggccgtggc aggtcggcgt gctctacaac gaggatgacc
     4741 gcgccgcgcg gatcgccatt cccctgctgg aggcggatgg gttggtggtg ggggaccagc
     4801 agccttattc cggcaagctg ctcaactaca cgatggaccg ccacgccgag gcggacgggc
     4861 ggccctatct cggcatcgag atccggcagg accagatcgc cgatcccgcc gggcaggccg
     4921 aatgggccga gcgtctggcc cggatcgcca accgggttgc gctggaactg ggatagccac
     4981 ctggcgaggg acttgcattt acattaggga cgccttaaat gcggtatgtc taatttaaag
     5041 ggttccacca atgtctgttc cttcgctgca tcccgacgaa gcccggcgcc ttatcgagca
     5101 gggcaaggcg gtcctgatcg atatccgcag cccgcaagag cacgcgcgcg agcggatccc
     5161 gggcgcggtc tgcattccgc tggaccagct ccagcaggcg gcaggcccgg tcccggaagg
     5221 cccggtggtc tttcactgcc gcagcggcat gcgcaccgat gccaaccgcg cggtcctggc
     5281 ccaggccagc gccggccagc cgtgctatgt gctggagggc gggatcgagg ggtggaagcg
     5341 gcacggccac tccaccctgt ttgaccgctc gcagccgatc gacatcatgc gccaggtcca
     5401 gattgcggcc ggaacgctgg tcctgatcgg ggtgctgggc ggtgtcttcg tcaccccgct
     5461 gctgtacggt ctggccgggt tcgtcggcgc gggcctgacc tttgccggcc tcagcgggtt
     5521 ctgcggcatg gcgcggctgc tggccctgat gccgtggaac cgcgcggcgc aaggggcttg
     5581 acggcggcac tggtctcaac tgaaacgttc gacgctagcc cgcggcttcg ctaggggctt
     5641 atccgccgtc cgcgccgcgg cggttcagcg ttggagtttg caatgcccgt ctaccgttcc
     5701 cgtacgtcca cccatggccg caacatggcc ggcgcgcgcg gattgtggcg ggcaacgggc
     5761 atgaaggacg gcgatttcgg caagccgatc atcgcggtgg tgaactcctt cacccagttc
     5821 gtgccgggcc acgtccacct taaggacctg ggccagatgg tcgcgcgcga gattgaggca
     5881 gcgggcggtg ttgccaagga attcaacacc atcgcggtcg atgatggcat cgccatgggc
     5941 cacgacggga tgctctattc gctgcccagc cgcgatctga ttgccgacag cgttgaatac
     6001 atggtcaacg cccactgcgc cgatgcgatg gtctgcattt ccaactgcga caagatcacg
     6061 cccggcatgc tgatggc
//
LOCUS       DASUDV010000524         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1719220,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000524 DASUDV010000000
VERSION     DASUDV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719220"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFV30_11455"
     CDS             <1..397
                     /locus_tag="VFV30_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX4848753.1"
                     /translation="ACMDPFLAALSAAAFTLCGDAPRINCVVDGDTFWIAGEKVRLAD
                     INAPETHQAGCAAERELGVRATRRLIALLNAGPFTLEIEGRGTDRYGRALRVARRGGS
                     SIGQQLVREGLAEPWRGRRSDWCILLARD"
     gene            419..991
                     /locus_tag="VFV30_11460"
     CDS             419..991
                     /locus_tag="VFV30_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848754.1"
                     /translation="MAWPRRKTGYGRVLPTHRWQGQPRRARLWHTLKWWLGAALLVAA
                     MAWISGYWPGRIAPAEMPGGPAEVLSGPFVRCGKGRAAGCVPDGDTLIVGKRTIRIIG
                     IDAPELHPARCPAEAEKGEAAARALLTLLNQGPVTLAGPVPPVRDEYGRELRHLLRAR
                     PDGTVQSIADDLVAGGTVRPYLHGSRDPWC"
     gene            1019..1321
                     /locus_tag="VFV30_11465"
     CDS             1019..1321
                     /locus_tag="VFV30_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX4848755.1"
                     /translation="MLLIEGWIKLATGEFEKVREQGIAMVKATNEEAGCLHYAFAQDI
                     ADPDLIRISERWESQEALAAHSASAHMAEFNKAMAGVQREGADLWLYSAEAVRKLI"
     gene            complement(1318..2073)
                     /locus_tag="VFV30_11470"
     CDS             complement(1318..2073)
                     /locus_tag="VFV30_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4848756.1"
                     /translation="MKLGRRKLLGLGAAGAGGLLLGGCDRLNGSPDFRAGLASTEGLT
                     MRVQRLLMGDALAREFTEAEMSPSFRVNGNSRVADSAYRRLLDAGFAGWALRVDGLVQ
                     RPLDLTLDAIKGLPQRTQITRHDCVEGWSAIGKWQGPALGPILAAAGLLPNARYAVFH
                     CADRFGDKPYYESIDLIDAFHPQTILAWAMNGAPLSEGHGAPVRLRVERQLGYKHAKF
                     VMRIELRESLAGLYGGKGGYWEDNRDYAWYAGI"
     gene            complement(2070..2849)
                     /locus_tag="VFV30_11475"
     CDS             complement(2070..2849)
                     /locus_tag="VFV30_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX4848757.1"
                     /translation="MSQPPADDPPAAEPPAPQGGDLVKRHSLITRLWHWANAMTAAIM
                     LMSGLMIFNAHPRLYWGHYGANADPAWLDLSHINGGVPFPGWATIPTAYSLSDARLWH
                     LSFGWLLVLALLGYLLWALLSGRLIKALLPRLAELRPAHLWHDIAEHARLRFPGGAAA
                     LRYNVLQKLAYSAVLLVLLPGLVLTGLGMSPAMDAAWPWLLDLLGGRQSARSLHFLCA
                     AGLVGFFVVHIAMVVLAGPFNELRSMLSGWYRLPPERKDAP"
     gene            2988..3848
                     /locus_tag="VFV30_11480"
     CDS             2988..3848
                     /locus_tag="VFV30_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4848758.1"
                     /translation="MPFVAVAAGIASFSLMDGLMKGASLAIGAYSAMLWRSLAGIVIM
                     APVWKLGGGRWPDRAVMKLHAVRGLNSAAMATTFFWGLKYLPLAEGMAISFIAPLIAL
                     YLAAVTLGETIGRKAVIASILGLAGVLVIVAGRFGESGYDRDAAWGIAAILTSAVLYA
                     WNLILQRRQAQIADPREIAMFQAVFCGLFLAVLAPWLLQVPTPAVWIDLIGSAVLAAI
                     SLMLIAWGYAREETQVLLPLEYSAFIWAALLGWVMFAEALTWPTVAGAVLIVAGCLMA
                     TRKHIEQTAL"
BASE COUNT          632 a         1387 c         1428 g          604 t
ORIGIN      
        1 cgcgtgcatg gacccgtttc ttgctgccct ttccgctgcc gccttcaccc tgtgcggcga
       61 tgccccccgg atcaactgcg tggtcgatgg cgacaccttc tggatcgccg gagagaaggt
      121 gcggctggcc gacatcaacg cgcctgaaac ccaccaggcc ggctgcgcgg ccgaacgcga
      181 gctgggcgtg cgtgccaccc ggcggctgat cgccctgctc aacgccgggc ctttcacgct
      241 ggagatcgag ggccgcggga cggaccgcta tggccgcgcg ctgcgggtgg cgcggcgcgg
      301 cggcagttcg atcggccagc agctggtccg cgaagggctg gccgagccgt ggcgcgggcg
      361 gcgatcggac tggtgcatcc tgcttgcccg ggactgatcc cggccctacc cagcggcgat
      421 ggcctggccc cgccgcaaga caggatacgg acgcgttctg cccacccacc gctggcaagg
      481 ccaaccgcgg cgggcgcggc tgtggcacac gctcaaatgg tggctgggcg ccgcgctgct
      541 ggtggcggcg atggcgtgga tttcaggcta ttggccgggc cggatcgcgc cggcggaaat
      601 gcccggcggc ccggctgaag ttctcagcgg ccccttcgtg cgctgcggca agggccgcgc
      661 ggcgggctgc gtgcccgatg gcgatacgct gatcgtgggc aagcggacca tccggatcat
      721 cgggatcgac gcacccgaac tgcacccggc gcgctgcccg gccgaggcgg agaagggcga
      781 ggccgccgcc cgggcgctgc tcaccctgct taaccagggc ccggtcacgc tggccggccc
      841 cgtcccgccg gtacgcgacg aatacgggcg cgagctgcgc cacttgctcc gcgcccggcc
      901 tgacggcacc gtccagtcga tcgccgacga tctcgtcgcg ggcggaaccg tgcgtcccta
      961 cttgcacggc agccgcgatc cgtggtgtta aaggcccaag tgaacagagg agagtggaat
     1021 gctgctgatc gaaggctgga tcaaactggc gaccggggaa ttcgaaaagg tccgcgaaca
     1081 ggggatcgcc atggtcaagg cgaccaacga agaggccgga tgcctgcact atgccttcgc
     1141 ccaggacatt gccgatcccg accttatccg catttccgaa cgctgggaaa gccaggaagc
     1201 cctcgccgcc cattccgcca gcgcacacat ggccgaattc aacaaggcga tggccggcgt
     1261 gcagcgcgaa ggcgcggacc tgtggctcta cagcgcggaa gcggtccgca agctgatcta
     1321 gatcccggcg taccaggcgt agtcgcggtt gtcctcccag tagccgccct tgccgccgta
     1381 gagcccggcc aggctttcac gcagttcgat ccgcatcacg aacttggcgt gcttgtagcc
     1441 aagctggcgc tcgacccgca gccgcaccgg cgcgccgtgg ccttcggaga gcggcgcgcc
     1501 gttcatcgcc caggccagga tcgtctgggg gtggaatgcg tcgatcagat cgatcgattc
     1561 gtaatagggc ttgtccccga accggtcggc gcagtggaac acggcgtagc gggcattggg
     1621 cagcaggccg gccgcggcga ggatcgggcc gagcgcgggt ccctgccact tgccgatcgc
     1681 gctccacccc tcgacacagt catgccgggt gatctgggtg cgctggggca ggcccttgat
     1741 ggcgtcgagc gtcaggtcga gcggccgctg gaccagcccg tccacccgca gcgcccagcc
     1801 ggcaaagccc gcgtccagca ggcggcggta ggcgctgtcg gcaacccggg aattgccgtt
     1861 gacccggaag ctgggcgaca tctccgcctc ggtgaactcg cgcgccaggg catcgcccat
     1921 cagcagccgc tggacccgca tggtcagtcc ttcggtgctg gccaggccgg cgcggaaatc
     1981 ggggctgccg ttcagccggt cacacccgcc cagcagcagc cccccggccc cggccgcgcc
     2041 cagccccagc aacttgcggc ggcccagttt catggcgcgt cctttcgttc gggcggcagg
     2101 cggtaccagc cgctcagcat cgagcgcagt tcgttgaagg gtccggccag caccaccatc
     2161 gcgatgtgga ccacgaagaa gccgaccagc ccggccgcgc agaggaagtg gagcgagcgc
     2221 gccgattgcc gcccgcccaa gaggtcaagc agccacggcc aggccgcgtc catcgccggc
     2281 gacatgccga gcccggtcag caccagcccc ggcagcagca ccagcaggac cgcgctgtag
     2341 gccagcttct gcagcacgtt gtagcggagc gccgcggccc cgccgggaaa acgcagccgg
     2401 gcgtgctcgg caatgtcgtg ccacaggtgc gcggggcgca gttcggcgag gcgcgggagc
     2461 agcgccttga tgagccgccc cgaaaggagc gcccagagca gatagcccag caaggccagg
     2521 accagcagcc agccgaacga gaggtgccac agccgcgcat cggacaggct gtaggcggtg
     2581 gggatcgtgg cccagccggg gaacggcacc ccgccgttta tgtggctgag atcgagccag
     2641 gccggatcgg cgttggcgcc gtaatggccc cagtacagcc gcgggtgggc gttgaagatc
     2701 atcagcccgc tcatcagcat gatcgcggcg gtcatcgcgt tggcccagtg ccacagccgg
     2761 gtgatcaggg agtgacgctt taccagatcg ccgccttgcg gggcgggcgg ctctgcagcg
     2821 ggcggatcat cggcaggcgg ctgggacatg tcctctcctc gcccgcgcag cctagggcct
     2881 tgcagcgggc ggcgcaaccg cgcgcccttg cggcggcggt ccggctcccc taccgctggc
     2941 cccgggggag accggccttg ccaagcggcg acagacacca tcacctgatg ccgttcgtgg
     3001 cggttgccgc cgggattgcc agcttttcgc tgatggacgg cctgatgaag ggcgccagcc
     3061 tggcgatagg ggcctattcg gcgatgctgt ggcgctcgct cgcagggatc gtgatcatgg
     3121 ccccggtatg gaagctgggc ggcgggcgct ggcccgaccg ggcggtgatg aaactccacg
     3181 ccgtgcgcgg tctcaacagc gcggccatgg cgaccacgtt cttctggggg ctcaaatacc
     3241 tgcccttggc ggaaggcatg gcgatatcct tcattgcccc gctgatcgcg ctctatctgg
     3301 cggcggtgac gctgggcgaa acgatcggcc gcaaggcggt aatcgcctcg atcctggggc
     3361 tggccggggt gctggtgatc gtggccggac ggttcggcga aagcggttat gaccgcgacg
     3421 ccgcctgggg catcgccgcg atcctgacct cggccgtgct ctatgcctgg aacctgatcc
     3481 tccagcgccg ccaggcccag atcgccgacc cgcgcgagat cgcgatgttc caagcggtgt
     3541 tctgcggcct gttcctggcc gtgctggcgc cctggctgct gcaggtgccg accccggcgg
     3601 tgtggatcga cctgatcgga tcggccgtgc tggcggccat atcgctgatg ctgatcgcct
     3661 ggggctatgc ccgcgaggaa acgcaggtcc tgctgccgct ggaatacagc gccttcatct
     3721 gggccgcgct gctgggctgg gtgatgttcg ccgaggcgct gacctggccg accgtggcgg
     3781 gcgcggtgct gatcgtggcc ggatgcctga tggccacccg caagcacatc gaacagacgg
     3841 cgctttagtt gtccgcctga tggcggtgcg ggaaagccat ccggctttcc ctacctggcc
     3901 ggcccgctcc ccctgcccaa ccaccccaag ggtaccctcg aacgggtggt tgggcagggg
     3961 gagcgggccg gtgttgccaa gccaatcgcg gaggcgattg gcgcacccaa ccgaagactc
     4021 ccaccgacaa aggtctaata tcgcgccgaa c
//
LOCUS       DASUDV010000525         5538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1431952,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000525 DASUDV010000000
VERSION     DASUDV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431952"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFV30_11485"
     CDS             complement(<1..362)
                     /locus_tag="VFV30_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848759.1"
                     /translation="MTLRPFLLALIALPLLGAAPPVRTMTCAVPVKKGDTAASLKARY
                     GAQAKLMKIHAAEGEMVNGMALWPNDPARRIDVFFAEVPGRKVETIRLTGTRSAWRIG
                     GLGIGSRLDAVVKANGRPV"
     gene            523..1623
                     /locus_tag="VFV30_11490"
     CDS             523..1623
                     /locus_tag="VFV30_11490"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX4848760.1"
                     /translation="MATLAESRPAFTRLPHPGPVADSVRAEILANPGFGSRFTDHMVV
                     IEWTEEAGWHGAQVRPYGPSALDPAASVLHYAQEIFEGLKAYRHPDGSMALFRPEANA
                     ARFNASARRLAMPELPEALFIAAIEELVRADAGWFPSVEGGSLYLRPFMIATEAFLGV
                     RPAKAYKFIVIASPAGNYFKSGAPAVSIWVSDYTRAAPGGTGAAKCGGNYAASLVPTA
                     EAFAKGHDQVLFLDAAEHKWIEELGGMNLFFAFADGRLTTPPLTGTILPGITRDSLIQ
                     LAREEGLDVREERYSLDQWRADAASGALVEAFACGTAAVVTPVGKVADHGGDFTIGSG
                     GPGQLTGKLKARLTAIQRGEEPDSHGWVRRIG"
     gene            1842..3806
                     /locus_tag="VFV30_11495"
     CDS             1842..3806
                     /locus_tag="VFV30_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848761.1"
                     /translation="MVRVPPPPPLRLNLAVTGHRETHPAYAANSAAIRAVFERLFALI
                     DATVTAERASLGTVDPVRLHCLLADGTDQLAAELGIARGWDLACPLPFGRDLNCAVNA
                     HPGTRADAEAILAGNDPADPDCRRRAAAIRTWYGQARVFELADHDAVLSRQFLEIFDR
                     PDDRTAQTLYSAGSSAQVALAGRIMVEQSDLLLAVWDGVSANLAGGTGHTIAAALDRG
                     CAVVRIDPANPEAWHLLHAPESLAAMPARGDDETTLAALVRAALRPGEGGALRSGAET
                     LAGEHWHGQSPRLWTGYRRIEALFGGGGRPFRSLITTYESPEAIAAGSGAAVLEAART
                     LPAGDTAMPGQIAAKVLERFAWTDGISARLSDSYRGSMTTSFLLSALAIVCGIAYEPF
                     GGLERKWLFALGEFLLLGAILVIIWQGGKRRWHKRWFETRRVAEYFRHAPILLLLGVA
                     RPPGRWPKGSDTSWPEYYARHALRELGLPQVAVTKAYLRHALDHLLDAHVVSQRDYHR
                     FKSKRLNTVHHRLDRLSERLFQAAIISVSIYLTVAGGAALGLLDHGTLYVTSKAASFL
                     GVAFPTFGAALAGIRYFGDFERFAAISEVTAEKLDAIHDRIQLLQAAPDHGLTYAQVS
                     ELVHAADDAVVAEIESWQAVFGGKHITVPV"
     gene            3830..4798
                     /locus_tag="VFV30_11500"
     CDS             3830..4798
                     /locus_tag="VFV30_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX4848762.1"
                     /translation="MDEFDEIKADVIKSRTQFANMPDSFWLMMLVVVVILFAAGIRIG
                     GINVPLERIPWSFLVPCMALFCFVHSTIMLGLKRALTLMGLAVSISFCAEYIGESTGI
                     LFGPYCYTGVLGPKILDRVPVLIPFAWYMMFYPSYVVTNILAEARPVSGRHDTTWIVW
                     VSLLSALIMTAWDITMDPVMSYYPDGGHAKWCDAADLHEANVGHPAWVWIDGGVHFGV
                     PLRNFAGWMLTAFVVFLIYRLLERKLPLRPLQGYRSRMMAFLPVAAYGMMALIDAWLG
                     YPEIEDIHLISPFVMGIPFLFASFKLFAQRTDIPLWPWQHKVEDDA"
     gene            4791..>5538
                     /locus_tag="VFV30_11505"
     CDS             4791..>5538
                     /locus_tag="VFV30_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX4848763.1"
                     /translation="MPREKIAILGGGVGSLITAWELTATPELRAKYDVTLYQMGWRLG
                     GKGATGRNLDPGKGLRIEEHGLHVWLGFYDNAFQVIQDAYAEVKARGLAPDSPFQQWS
                     DAFKPQSFTPVGVGDGSTWVPVTWPTNPLEPGIHPISALKAIEAALDMLGQLIKMVFG
                     KNPLRALCIGWRLLRIRQAAGKLSSEAALDNEAHLRTIHHHLDAMAAQLRKGAKGNTD
                     PGVIAALVLEMIEMGLACLRGFLNPEYGLLK"
BASE COUNT          840 a         1949 c         1850 g          899 t
ORIGIN      
        1 accggccggc cgttggcctt gaccaccgca tcgagccgcg agccgatccc cagcccgccg
       61 atccgccagg ccgaacgggt cccggtcagg cggatcgttt ccaccttgcg ccccggcact
      121 tcggcaaaga acacgtcgat ccggcgggcc gggtcgttcg gccagagcgc catgccgttc
      181 accatctcgc cctcggcagc atggatcttc atcagtttcg cctgggcgcc gtagcgggcc
      241 ttgaggctgg ccgccgtatc gcccttcttg accggcacgg cgcaggtcat ggtccgcacg
      301 ggcggagccg cgcccagcag cggcagggca atcagggcca gcaggaacgg gcgcagcgtc
      361 atggcgggat ctcctttgcc ggcatcctgc ggcggcttgg ccttttgcgc aatcccggct
      421 atgcaggatg tagaaatagg gtcttttcga taattacctt tcgcttgcgc ggcgcgtaat
      481 tatcattcaa agccaccgtt cgcagttgaa ggatcgaagc ccatggcgac cctcgccgaa
      541 tcgcgccccg cgttcacccg cttgccccac cctggcccgg tggccgattc cgtgcgggcc
      601 gagatcctcg ccaaccccgg cttcggcagc cgctttaccg atcacatggt ggtgatcgaa
      661 tggaccgaag aggccggctg gcacggcgcc caggtccgcc cctatggccc gagcgcgctc
      721 gatccggcgg cttcggtgct gcactatgcc caggaaatct tcgaagggct gaaggcctac
      781 cgccaccccg atggttcgat ggcgctgttc cggcccgaag ccaacgccgc gcgcttcaac
      841 gcctcggccc ggcggctggc catgcccgaa ctgcccgaag cgctgttcat cgccgcgatc
      901 gaggaactgg tccgggccga cgccgggtgg ttccccagcg tcgagggcgg ctcgctctat
      961 ctccgcccct tcatgatcgc gaccgaggcg ttcctcggcg tgcgcccggc caaggcctac
     1021 aagttcatcg tcatcgccag cccggcgggg aactatttca agtctggcgc gccggcggtc
     1081 tcgatctggg tgtcggacta cacccgcgcc gccccgggcg gcaccggcgc ggccaagtgc
     1141 ggcggcaact atgccgccag cctggttccc accgccgaag cctttgccaa ggggcacgac
     1201 caggtgctgt tcctggacgc ggccgagcac aagtggatcg aggagctggg cggcatgaac
     1261 ctgttcttcg cctttgccga cggccgcctg accaccccgc cgctgaccgg cacgatcctg
     1321 cccgggatca cccgcgacag cctgatccag ctggcccgcg aggaagggct ggacgtgcgc
     1381 gaggagcggt acagcctgga ccagtggcgc gccgacgccg cgtctggcgc gctggtcgaa
     1441 gcctttgcct gcggcaccgc cgcggtggtg accccggttg gcaaggtggc cgatcacggc
     1501 ggggacttca ccatcggcag cggcggcccc ggccagctga ccggcaagct caaggcccgg
     1561 ctgaccgcga tccagcgcgg cgaagagccg gacagccacg gctgggtccg ccggatcggc
     1621 tgatccgccc cgcgcggcgc cgatcctgcg ccgctttgcc gattgcatct gatccgcgcg
     1681 ccgctaagct gcggtaacgg gcgcgaaatc gcggttcggc ctatcccggc cggccgcgcc
     1741 ggatcggtgt gggggacaat ggcagacgat cggcggacag cctggaatcc ggaagggttg
     1801 cgcttgcgcg cggccccctc ggccgaacgg gcgtgaccgc gatggtccgg gtgcccccgc
     1861 cgccgccgct ccggctgaac ctggccgtca ccggccaccg cgaaacccat cctgcctatg
     1921 ccgccaacag cgccgcgatc cgcgcggtgt ttgaacggct gttcgccctg atcgacgcca
     1981 ccgtcacggc cgaacgcgcc agcctgggca cggtcgaccc ggtccggctg cactgcctgc
     2041 tggccgacgg gaccgaccag ctggcggccg agctgggcat tgcccgcggc tgggacctgg
     2101 cctgcccgct gccgttcggg cgcgatctca actgcgcggt caacgcccac ccgggcaccc
     2161 gcgccgatgc cgaggcgatc ctggccggca acgatccggc cgatccggac tgccgccgcc
     2221 gcgccgccgc gatccgcacc tggtacggcc aggcccgggt gttcgaactg gccgatcacg
     2281 acgcggtgct gagccgccag ttcctggaaa tcttcgaccg gccggacgac cgcacggcgc
     2341 agacgcttta ctcggccgga tcctcggccc aggtcgcgct ggccgggcgg atcatggtcg
     2401 aacagtccga cctgctgctg gcggtgtggg acggggtgtc tgccaacctg gccggcggaa
     2461 ccggccacac catcgccgcc gcgctggacc gcggctgcgc ggtggtgcgg atcgatccgg
     2521 ccaatcccga ggcctggcac ctgctccacg cacccgaatc gctggccgcc atgccggcgc
     2581 ggggtgatga cgagacgacc ctggccgccc tggtccgcgc cgcgctgcgg cctggcgaag
     2641 gcggggccct gcgcagcggc gcggaaaccc tggcgggcga gcactggcac ggccagagcc
     2701 cgcggctgtg gaccggctac cgccggatcg aggcgctgtt cggcggcggc gggcggccgt
     2761 tccgttcgct tatcaccacc tacgaaagcc ccgaggcgat tgctgcgggc agcggcgccg
     2821 ccgtgctcga agccgcccgc accctccccg ccggcgacac cgccatgccc ggccagatcg
     2881 ccgccaaggt gctggaacgc tttgcctgga ccgacgggat ctcggcccgc ctttccgaca
     2941 gttaccgcgg cagcatgacc accagcttcc tcctgtcggc gctggcgatc gtctgcggca
     3001 tcgcttacga accgttcgga ggactggagc gcaagtggct gtttgccctc ggcgaattcc
     3061 tgctgctcgg cgcaatcctg gtgatcatct ggcaaggcgg caagcggcgc tggcacaagc
     3121 gctggttcga aacccgccgg gttgccgaat acttccgcca cgcaccgatc ctgctgctgc
     3181 tgggggtggc ccgcccgccc gggcgctggc ccaagggatc ggacaccagc tggccggaat
     3241 actatgcccg ccacgccctg cgcgaactgg gcctgcccca ggtggcggtg accaaggcct
     3301 atctgcgcca cgcgctcgat cacctgctcg atgcccacgt ggtcagccag cgcgattacc
     3361 accgcttcaa gtcaaagcgg ctcaacaccg tccaccaccg gctggaccgg ttgtcggaac
     3421 ggctgttcca ggccgcgatc atctcggtct cgatctatct gacggtcgcc ggcggcgcgg
     3481 cgctgggcct gctcgatcac ggcacgctct atgtcaccag caaggccgcc tcgttcctgg
     3541 gcgtggcctt ccccaccttt ggcgcggcgc tggccgggat ccgctatttc ggggacttcg
     3601 aacgctttgc cgcgatcagc gaagtgaccg ccgagaagct ggatgcgatc cacgaccgga
     3661 tccagctgct gcaggccgcg cccgatcacg ggctgaccta tgcccaggtg tccgaactgg
     3721 tccacgccgc cgatgatgcg gtggtggccg aaatcgaatc ctggcaggcc gtcttcggcg
     3781 gaaagcacat caccgttcca gtctagtcac accagcttcg ggggaagcca tggacgagtt
     3841 cgacgagatc aaggccgacg ttatcaagag ccggacccaa ttcgccaaca tgccggattc
     3901 gttctggctg atgatgctgg tggtggtggt gatcctgttc gccgccggga tccggatcgg
     3961 cgggatcaac gtgccgctgg aacggattcc gtggtcgttc ctggtgccgt gcatggcgct
     4021 gttctgcttt gtccattcca ccatcatgct gggcctgaag cgcgcgctta cgctgatggg
     4081 gctggcggtc tcgatctcgt tctgcgcgga atacatcggc gaaagcaccg ggatcctgtt
     4141 cggcccctat tgctacaccg gcgtgctggg cccgaagatc ctggaccggg tgccggtgct
     4201 gatccccttc gcctggtaca tgatgttcta ccccagctat gtcgtgacca acatcctggc
     4261 cgaggcgcgg ccggtgtccg ggcggcacga caccacctgg atcgtctggg tttcgctgct
     4321 cagcgcgctg atcatgaccg cctgggacat caccatggac ccggtgatga gctattaccc
     4381 cgatggcggc cacgccaagt ggtgcgatgc cgccgatctg cacgaggcca atgtcggcca
     4441 cccggcctgg gtgtggatcg acggcggcgt ccatttcggc gtgccgctgc gcaatttcgc
     4501 ggggtggatg ctgacggcct tcgtggtctt cctgatctac cgcctgctgg aacgcaaact
     4561 gccgctgcgc ccgctgcagg gctaccgctc gcggatgatg gccttcctgc cggtcgccgc
     4621 ttacgggatg atggcgctga tcgatgcctg gctgggctat ccggagatcg aggacatcca
     4681 cctgatctcg cccttcgtga tgggcattcc gttcctgttc gccagcttca agctgttcgc
     4741 ccagcgaacc gacatcccgc tgtggccgtg gcaacacaag gtggaagacg atgcctaggg
     4801 aaaagatcgc gatcctgggc ggcggggtcg gctcgctgat caccgcctgg gaactgaccg
     4861 ccacgccgga gctgcgggcc aagtacgatg tcacgctgta ccagatgggc tggcggctgg
     4921 gcggcaaggg cgcgaccggg cgcaacctcg atcccggcaa gggcctgcgg atcgaggagc
     4981 acggcctcca cgtctggctg ggcttctatg acaacgcgtt ccaggtgatc caggacgcct
     5041 atgccgaagt gaaggcccgg ggcctggcgc cggacagccc gttccagcaa tggagcgacg
     5101 cgttcaagcc gcagagcttc acgccggtgg gcgtgggcga cggcagcacc tgggtgccgg
     5161 tgacctggcc gaccaacccg ctggagccgg gcatccaccc gatcagcgcg ctgaaggcga
     5221 tcgaggccgc gctcgacatg ctgggccagc tgatcaagat ggtgttcggc aaaaacccgc
     5281 tgcgggcgct gtgcatcggc tggcggctgc tgcggatccg ccaggccgcc ggcaagctga
     5341 gcagcgaggc ggcgctggac aacgaagccc acctgcgcac gatccaccac catctggacg
     5401 ccatggccgc gcagctgcgc aagggcgcca agggcaacac cgatcccggc gtgatcgccg
     5461 cgctggtgct ggaaatgatc gagatggggc tggcctgcct gcgcggcttc ctcaaccccg
     5521 aatacggcct gctcaagg
//
LOCUS       DASUDV010000526         6315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_791307,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000526 DASUDV010000000
VERSION     DASUDV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791307"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFV30_11510"
     CDS             <1..361
                     /locus_tag="VFV30_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4848764.1"
                     /translation="ARLERVLVNLLDNACSFSPPEGRVDLMIGRQSDRLELSVTDEGP
                     GIPAEAREKIFDRFHSLRPEGEDFGSHSGLGLAIARTIAEAHDGTLTAHDRPDGQRGA
                     CLVLDLPLAEDDEEEDG"
     gene            364..783
                     /locus_tag="VFV30_11515"
     CDS             364..783
                     /locus_tag="VFV30_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4848765.1"
                     /translation="MLGGTSAVAIGGRALLIQGEPGSGKSGLALALIDRGAVLIGDDG
                     VTLEVREGRLLASPPPNTAGLLEVRNLGVLSWPTEAAVPVALVLRFDPEAPRFIEAAD
                     RLELGGLSVPLVRIWPDGPVPAIKAELALQRFGLAPN"
     gene            853..1767
                     /gene="rapZ"
                     /locus_tag="VFV30_11520"
     CDS             853..1767
                     /gene="rapZ"
                     /locus_tag="VFV30_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX4848766.1"
                     /translation="MTESSPPRQRVLLVTGLLGAGKTTVLKVLEDLGWEAIDNFPIRL
                     LGRLFDDGAGGPADLRAPLAIGFDSRTRGFDPAKVIDRVKKLVGRADLEVTTLFLDCA
                     GQELERRYNETRRRHPLAQDLPAASGIAAEREMMEPLRRWADLVISTTQFTSNDLQQA
                     IRERFAKDAPAEMTVSVSSFGFSRGTPPLADLVFDMRFLDNPHWDEALRPLTGRDKSV
                     ADHIRKDPAFADAFTRIRELIELLLPRYRAQGKAYVNIAFGCTGGRHRSVFVAEEITK
                     ALTKAGYSPSLVHRNLNARAADLVEGKH"
     gene            1850..2254
                     /locus_tag="VFV30_11525"
     CDS             1850..2254
                     /locus_tag="VFV30_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX4848767.1"
                     /translation="MIGLILVTHGKLAEEFVSAMQHVVGRQDAVKTVCIGPNDDMEAR
                     RAEIRTAVRDVDSGEGVIILTDLFGGTPSNLAISLMQKGKIEVIAGINLPMLIRLAKA
                     RGCMPLEKAVTAARDAGRNYITIASEFLGQDA"
     gene            2272..2550
                     /locus_tag="VFV30_11530"
     CDS             2272..2550
                     /locus_tag="VFV30_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX4848768.1"
                     /translation="MGEARETVEIVNNRGLHARASAKFVNAVTKLGDGVKVRVEKDGA
                     DAAGASILGLMMLGAAKGDSIDILVSGDGADAALTKLVGLVKDGFGED"
     gene            2554..3375
                     /locus_tag="VFV30_11535"
     CDS             2554..3375
                     /locus_tag="VFV30_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX4848769.1"
                     /translation="MAERRQITGFSNPTVKFLRSLREKKHRKASAKFLAEGLRLLTDA
                     RECGHLPEVLAMSTARDPHPLLDALEADVLAAGGEVLELTPDLLAKITGKDNPQAVAG
                     VFAEFDTGLAAIDRSSAKIWLVAQALRDPGNLGTMLRTGDAVGAGGLILIDDCADPFS
                     VEAVRASMGAVFTQKIAQARWEEFLPWLRSANGQLVAASLRDAVDYRTAPYAAPCFLM
                     VGNESRGLPEDYEAACDLRVTMPMKGRADSLNAAVAGAVLAYEALARLDSGIREI"
     gene            3419..3808
                     /locus_tag="VFV30_11540"
     CDS             3419..3808
                     /locus_tag="VFV30_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX4848770.1"
                     /translation="MIKRITLLALPLLAACATTVQAEPSLIGTKWVFVSIDGKAPVSD
                     RTELTIEQGRIGANVGCNGMGSDLRIEGDRLITNGVISTKMFCDAIAGQEMAVGALLD
                     ASPSYRIEGGRLILTSPGHRVELRRAN"
     gene            complement(3816..5180)
                     /gene="rmuC"
                     /locus_tag="VFV30_11545"
     CDS             complement(3816..5180)
                     /gene="rmuC"
                     /locus_tag="VFV30_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX4848771.1"
                     /translation="MDGQILGIVLLLLGLAVGALGGWFAGSRPAAELRDRLADAEAEG
                     RELDARFRKAVADLGNTSIELATLKANAAHFDEQKAALLTAQENLRKEFEAAGARILT
                     QAREQMMAEAQQRLSLSEEKSAERLKALLAPVDQRLKSYEEQVQALEKNRVDAFGQLA
                     GLIQSVREGQEAVKTEAARLGNSLTNAPKARGRWGERALQNVLEQCGLSEHTDFNLEH
                     SLDTDEGRLRPDAIVHVPGQKKLVIDAKVSLNAYQAAFECSDEAERKRHLDLHARSMR
                     GHIQTLGAKSYQSQFEEAPDYVVMFVPGEHFVAAALENDPELWDFAFHNKVLLATPTN
                     LVAIARTVAMVWRQDALANEAREIGRIGTEIYERIATASEHLRRVGGGLESAVNNYNK
                     FVGSFERNVLTSARRLRDKGIEIGKREAEDVPLVEAAPRHGRPDGEPALLAGEGGTLD
                     DTAD"
     gene            complement(5222..5788)
                     /gene="def"
                     /locus_tag="VFV30_11550"
     CDS             complement(5222..5788)
                     /gene="def"
                     /locus_tag="VFV30_11550"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX4848772.1"
                     /translation="MAIREIIEVPDPRLKVVSEPVTQFDDELKTLVEDMFETMYDAPG
                     IGLAAIQVGVPLRVLVIDLQPDDPDADPVPCDHDGHHHHHQPTKKEPRVFINPEILDP
                     SDDHSVYQEGCLSVPEIYAEVERPARIRARWQDLDGKVHEEEMDGLMATCLQHEMDHL
                     EGILFIDHLSRLKRGMALKKLEKLRKAA"
     gene            complement(5851..>6315)
                     /gene="recR"
                     /locus_tag="VFV30_11555"
     CDS             complement(5851..>6315)
                     /gene="recR"
                     /locus_tag="VFV30_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX4848773.1"
                     /translation="ALEAVRVGLTECQTCGNVDTTNPCGICTDARRDARALCVVEDVA
                     DLWALDRARLFTGRYHVLGGRLSALEGVRPEDLTIGKLLERVSQGGIDEVVLAMNATL
                     EGQTTAHYIAERLEGYPVRITQLAHGLPVGGELDYLDEGTLAQALRARRPVG"
BASE COUNT          994 a         2166 c         2119 g         1036 t
ORIGIN      
        1 ggcccggctg gaacgggtgc tggtcaacct gctcgacaac gcctgctcgt tctccccgcc
       61 ggaaggccgg gtcgatctga tgatcgggcg gcaaagcgac cggctggagc tgtcggtcac
      121 ggatgaaggc ccgggcattc cggccgaggc gcgcgagaag atctttgacc ggttccactc
      181 gctgcggccc gaaggcgagg atttcggcag tcactccggc cttggcctgg cgatcgcgcg
      241 gaccatcgcc gaagcgcacg acggcaccct gaccgcccac gaccggcccg atggccagcg
      301 cggcgcctgt ctggtgctcg acctgccgct ggccgaggac gacgaggaag aagacgggtg
      361 agcgtgctgg gcgggaccag cgcggttgcg atcggcgggc gcgctctgct gatccagggc
      421 gagcccggca gcgggaaatc gggcctcgcc ctcgccctga tcgaccgcgg cgcggtgctg
      481 atcggggacg acggggtgac gctggaggtg cgcgaaggcc gcctgcttgc cagcccgccg
      541 cccaacaccg cgggcctgct tgaagtgcgc aacctgggcg ttctctcctg gccgaccgaa
      601 gccgcggtgc cggtggcgct ggtgctgcgc ttcgatcccg aggcaccccg ctttatcgag
      661 gcggcggacc ggctcgaact gggcgggttg tccgtgccgc tggtgcggat ctggccggac
      721 ggcccggtgc ccgcgatcaa ggccgaactg gcgctgcagc gcttcgggct ggccccgaac
      781 tgacccagcg ggggacttcc cctttggtcc cccttcacat tcgccgcggg gatgcgcatc
      841 ttggcaccag cgatgaccga atcctcccct ccccgccagc gcgtgctgct ggtcaccggt
      901 ctgctcgggg ccggcaagac caccgtgctc aaggtgcttg aagacctggg ctgggaagcg
      961 atcgacaatt tcccgatccg gctgctgggc cgcctgttcg atgacggggc cggcggcccg
     1021 gccgatctgc gcgcgccgct ggcgatcggg ttcgattcgc gcacccgcgg gttcgatccg
     1081 gccaaggtga tagaccgggt gaagaagctg gtcgggcgcg cggatctgga agtgaccacc
     1141 ctgttcctcg actgcgccgg gcaggagctg gagcggcgct acaacgaaac ccgccgccgc
     1201 catccgctgg cgcaggatct gcccgcggcg agcgggatcg cggccgaacg cgaaatgatg
     1261 gagccgctgc ggcgctgggc cgatctggtg atcagcacca cccagttcac cagcaacgat
     1321 ctgcagcagg cgatccgcga gcggtttgcc aaggacgccc cggccgaaat gaccgtcagc
     1381 gtgtccagct tcggcttctc gcgcgggacc ccgccgctgg ccgacctggt gttcgacatg
     1441 cggttcctcg acaatccgca ctgggacgaa gccctgcggc cgctgaccgg gcgcgacaag
     1501 tccgtggccg accatatccg caaggacccg gcctttgccg atgccttcac ccggatccgc
     1561 gagctgatcg agctgctgct cccccgctac cgcgcgcagg gcaaggccta tgtcaacatc
     1621 gcctttggct gcaccggcgg gcgccaccgc tcggtcttcg tggccgagga gatcaccaag
     1681 gcgctgacca aggccggcta ttcgcccagc ctggtgcacc gcaatctcaa cgcccgcgcg
     1741 gccgacctgg tcgaaggaaa gcattgaccg tgcgcgccgc ccgcgccatg gcccggcgcc
     1801 acccttcggc gcgaatcgcc gcgacgtcag ttcggagcgc agaacagcga tgatcggcct
     1861 gatcctggtc acccacggca agcttgccga agagttcgtt tcggcgatgc agcacgtcgt
     1921 cggccggcaa gacgcggtga agaccgtgtg catcggcccc aacgacgaca tggaagcccg
     1981 ccgcgccgaa atccgcaccg cggtgcgcga tgtcgattcc ggcgaaggcg tgatcatcct
     2041 gaccgacctg ttcggcggga ccccttcgaa cctggccatt tcgctgatgc agaagggcaa
     2101 gatcgaggtg atcgcgggga tcaacctgcc gatgctgatc cgcctggcca aggcccgcgg
     2161 ctgcatgccg ctggaaaagg ccgtgacggc cgcccgcgat gccgggcgca actacatcac
     2221 gattgcatcg gagtttctag gtcaggacgc ctgaggggga gtcgcggaac aatgggcgaa
     2281 gcgcgcgaga cagtggaaat cgtcaacaac cgggggctcc acgcccgggc cagcgcgaaa
     2341 ttcgtcaacg cggtgaccaa gctgggcgac ggggtgaagg tgcgggtcga aaaggacggc
     2401 gccgatgccg ccggggcctc gatcctgggc ctgatgatgc tgggcgcggc caagggcgat
     2461 tcgatcgaca tcctggtcag cggcgacggt gccgatgccg cgctgaccaa gctggtcggc
     2521 ctggtcaagg acggcttcgg cgaggattga tgcgtggctg agcgccgcca gataaccggc
     2581 ttttccaacc cgacggtcaa gttcctgcgg tcgcttcgcg agaagaagca ccgcaaggct
     2641 tcggccaagt tcctggccga ggggctgcgg ctgctgaccg acgcgcgcga atgcgggcac
     2701 ctgcccgaag tgctggcgat gagcaccgcg cgcgatcccc atcccctgct cgatgcgctg
     2761 gaggccgatg tcctcgccgc cgggggcgaa gtgctggaac tcacccccga tctgctcgcc
     2821 aagatcaccg gcaaggacaa cccccaggcg gtcgccgggg tctttgccga attcgacacc
     2881 ggcctcgccg cgatcgaccg gtcttcggcg aagatctggt tggttgccca ggcgctgcgc
     2941 gatccgggca acctcggcac gatgctgcgc accggcgatg cggtgggcgc gggcgggctg
     3001 atcctgatcg acgactgcgc cgatccgttc agcgtcgagg cggtgcgggc cagcatgggc
     3061 gcggtcttca cccaaaagat cgcgcaggcc cgctgggagg aattcctgcc gtggctgcgc
     3121 tccgccaacg gccagctggt cgcggcgagc cttcgcgatg cggtcgatta ccgtaccgcc
     3181 ccctatgccg cgccctgctt cctgatggtc ggcaacgaaa gccgcggcct gcccgaggat
     3241 tacgaggcgg cctgcgacct gcgcgtgacc atgccgatga agggccgcgc cgacagcctg
     3301 aacgccgccg tggccggggc cgtgctggcc tatgaggcgc tggcgcggct cgattccgga
     3361 atccgcgaaa tttgatctgt tcggttgcgg gcaaggttcg cgcgcctagt ctgatcccat
     3421 gatcaagcga atcaccctgc tcgccctgcc gctgctggcg gcctgcgcaa ccacggttca
     3481 ggcggagccg agcctgatcg gaacgaagtg ggtcttcgtt tcgatcgacg gcaaggcgcc
     3541 ggtttcggac cgcaccgaac tgaccatcga acaaggccgg atcggcgcca atgttggctg
     3601 taacggcatg ggttccgacc tccggatcga aggcgaccgg ctgatcacga acggggtcat
     3661 ttcgaccaag atgttctgcg acgcgatcgc cgggcaggaa atggcggtcg gcgcgctgct
     3721 cgatgcctcg cccagctacc ggatcgaagg gggccgcctg atcctgacct cgcccgggca
     3781 ccgcgtggag ctgcgccggg cgaactgagc ccgggtcagt cggcggtatc gtccagcgtg
     3841 ccgccttctc cggccagcag tgccggctca ccgtcgggcc ggccgtggcg gggcgcggcc
     3901 tcgaccagcg ggacatcctc ggcctcgcgc ttgccaatct cgatcccctt gtcgcgcagc
     3961 cgccgcgcgc tggtcagcac gttgcgttca aagctgccga cgaacttgtt gtaattgttg
     4021 accgcgcttt cgaggccccc gccaacacgc cgcaaatgct cggacgcagt ggcgatccgt
     4081 tcgtagattt cggtgccgat ccgcccgatc tcgcgcgcct cgttcgccag ggcgtcctgc
     4141 cgccagacca tcgccacggt ccgggcaatc gcaaccaggt tggtcggtgt ggcgagcaga
     4201 accttgttgt gaaaggcaaa gtcccacagc tcgggatcgt tttccagcgc cgcggcgacg
     4261 aagtgctcgc ccggcacgaa catcaccaca tagtccggcg cttcctcgaa ctggctctga
     4321 tagcttttcg cccccagcgt ctggatatgg ccgcgcatcg accgagcgtg gagatcgaga
     4381 tggcgtttgc gctccgcctc gtcgctgcat tcgaacgcgg cctgataggc gttgagcgag
     4441 accttggcgt cgatcaccag cttcttctgc cccggcacgt ggacaatcgc atcgggccgc
     4501 agccggccct cgtcggtgtc cagcgaatgc tcaaggttga agtcggtgtg ttcggacagg
     4561 ccgcactgct ccagcacgtt ctgcagcgcc cgttcgcccc agcggccgcg ggccttgggc
     4621 gcgttggtca gcgaattgcc cagccgcgcc gcctcggtct tgaccgcttc ctgcccttcc
     4681 cggaccgact ggatcagccc cgcaagctgg ccaaaggcat cgacgcggtt cttctccagc
     4741 gcctggacct gctcttcata gctcttcagc cgctggtcga ccggcgcgag cagcgccttc
     4801 aggcgttcgg cgctcttctc ttcagacagg ctcaggcgct gctgcgcctc ggccatcatc
     4861 tgctcgcggg cctgggtcag tatccgggcc ccggcggcct cgaactcctt gcgcaggttc
     4921 tcctgcgcgg tcagcagcgc ggccttctgc tcgtcgaaat gggcggcgtt ggccttcagc
     4981 gtggcgagct cgatcgaagt gttgccaaga tcggccaccg ccttgcgaaa gcgcgcgtcc
     5041 agctcccgcc cctcggcctc ggcatcggcc agccggtcac gcagttcggc cgccgggcgc
     5101 gaaccggcaa accagccgcc cagcgccccc acagcgaggc ccagcaaaag caatacgatg
     5161 ccaagaatct gtccgtccat tccgccacgc taaccggaac ggagcaagaa cgccagtcct
     5221 atcaggccgc cttgcgcagc ttttccagct tcttgagcgc catgccgcgc ttcaggcgcg
     5281 acaggtggtc gatgaacagg atgccttcga ggtggtccat ctcgtgctgg aggcaggtgg
     5341 ccatcaggcc gtccatctct tcctcgtgca ccttgccgtc cagatcctgc cagcgggcgc
     5401 ggatccgggc cgggcgttcg acctcggcat agatctccgg caccgaaagg cagccttcct
     5461 ggtagaccga gtgatcgtcc gacggatcga ggatttccgg gttgatgaac acccgcggtt
     5521 ccttcttggt cggctggtgg tgatggtggt ggccatcgtg atcgcagggc accggatcgg
     5581 catcgggatc gtccggctgg agatcgatca ccagcacgcg cagcggcacg ccgacctgaa
     5641 tcgcggcgag gccgatgcca ggggcatcgt acatcgtctc gaacatgtcc tcgaccaggg
     5701 tcttcagctc gtcgtcgaac tgggtgacgg gttcggacac caccttgagg cgcgggtcgg
     5761 gaacttcgat gatttcgcgg atagccatgc cggccagata gtcacgatgc gcgccaatcg
     5821 caagcggacg cgcacgatca gagtaatcgg ctagcccacc ggccgccgcg cgcgcagggc
     5881 ctgggccagg gtgccctcgt cgagatagtc cagctcgccc cccaccggca gaccgtgggc
     5941 cagctgggtg atccgcaccg gatagccttc cagccgctcg gcgatgtagt gcgcggtggt
     6001 ctggccttcc agcgtggcgt tcatcgccag caccacctcg tcgatcccgc cctggctgac
     6061 gcgttcgagc agcttgccga tggtcaggtc ctccggccgc acgccttcca gcgcggagag
     6121 ccgcccgccc agcacgtgat agcgcccggt gaacagccgc gcccggtcga gcgcccacag
     6181 gtctgccacg tcctccacca cgcagagggc gcgggcatcg cggcgggcat cggtgcagat
     6241 gccgcagggg ttggtggtgt cgacgttgcc gcaggtctgg cactcggtca gccccacccg
     6301 caccgcttcc agcgc
//
LOCUS       DASUDV010000527         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_636741,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000527 DASUDV010000000
VERSION     DASUDV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636741"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFV30_11560"
     CDS             complement(<1..795)
                     /locus_tag="VFV30_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848774.1"
                     /translation="MNAELPGSPIAKPRRSKRGCGCLVAPLILALLAAMLLWSRANRE
                     NPGAHPIPGASDAKVTVAPDFTPSPLPGAPFEHPYLAAAASGTMHGDGYQSDTHPAAG
                     PFGTGIEVRSRRSGNGLPRQCSTFVYRSDGKPVMMCGGAAGFRMVLLDPGSLEALAWY
                     DLPIRPSGFQALVKRDMSIMMNDSSGGAYMFLDNRDRLVFANSRQVVQRLEAVQQGGR
                     WAWRVEKEWDLKPFVPNDCMNWDNWFPSGECDMVTTVMPDTKGRYWW"
     gene            complement(792..1421)
                     /locus_tag="VFV30_11565"
     CDS             complement(792..1421)
                     /locus_tag="VFV30_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4848775.1"
                     /translation="MTDSALLARIDRLESLDAIRQLPAKYALALDMRDMEAMVSLFPA
                     DVRVGKDASGRQALRAYMDRTLRSPFTGTSHHIGGHVIEFDDADHAHGIVYSKNEHET
                     GDEWVIMQMMYVDDYVRNDGRWYFARRLPLYWYATDLNKPPIGANKMRWPGTDWAEGN
                     FHKLFPSYAEFWARDGDPGGPVAEPAPLEQFLNTMRRGAAAPKVKVRAD"
     gene            complement(1418..>2424)
                     /locus_tag="VFV30_11570"
     CDS             complement(1418..>2424)
                     /locus_tag="VFV30_11570"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX4848776.1"
                     /translation="AMGSRYYTLAFMDFYSAPHHLGTRTNRGEARRYALLGPSGGNVP
                     DGYEAFSLPTDTAWMLGRIMAEGRADEAEAKRLAQSIRMIGKPGEPVTNAEPLNPVAS
                     LEYFIVLNQALKGLPRQAGEEALMAQFDRAGFGPSATFDPDKLEPAQALGLGCAVRIG
                     PQVLAKTAFKPSFTATGWLQTRDLSNPGSDYLLRAEVVRGGYVNAREESIYPAAVMDS
                     QRRPLNGASAYRIRFPKGQLPPVDAFWSITPYAAPGMQLTENALRRYSIGSRTKGLKY
                     AADGSLTLTLSASRPRSGPANWLPVPPGSFTLVARLYLPRAEALDGRYVLPPFERIEP
                     "
BASE COUNT          420 a          758 c          817 g          429 t
ORIGIN      
        1 ccaccagtag cggcccttgg tgtcgggcat aactgtcgtc accatgtcgc attcgcccga
       61 tgggaaccag ttgtcccagt tcatgcaatc gttgggaacg aagggcttga gatcccattc
      121 cttctcgacc cgccaggccc agcgtccgcc ttgctgcacc gcttccagcc gctgcaccac
      181 ctggcggctg ttggcgaaga ccagccggtc gcggttgtcg agaaacatat aggccccgcc
      241 cgaactgtcg ttcatcatga tcgacatgtc gcgcttgacc agcgcctgga atcctgaagg
      301 ccggatcggc aggtcgtacc aggccagcgc ctcaaggctg cccggatcga gcaggaccat
      361 gcggaagccc gctgccccgc cgcacatcat caccggcttg ccatcgctgc gatagacaaa
      421 ggtcgaacac tggcgcggca ggccattgcc cgatctgcgg ctgcgcacct caatcccggt
      481 gccgaacggt ccggctgcgg gatgcgtgtc ggactgatag ccatcgccgt gcatcgtgcc
      541 gctggcggcc gcggcgagat agggatgctc gaacggcgcg ccaggcagcg gcgagggggt
      601 gaaatctgga gcaactgtta ctttggcgtc agacgcgccg gggatcgggt gcgccccagg
      661 gttctcgcgg ttggcgcggc tccacagcag catcgccgca agcagcgcca ggatcagcgg
      721 cgcgaccagg cagccgcagc cgcgtttgga cctgcggggc ttggcgattg gcgaaccggg
      781 caactcggcg ttcaatccgc gcgcaccttg accttgggtg cggccgcacc gcgccgcatg
      841 gtattgagaa actgctcaag cggagcgggc tcggccaccg gaccgccggg atcgccatcg
      901 cgtgcccaga attcggcata gctgggaaac agcttgtgga agttgccttc ggcccaatcg
      961 gttccgggcc agcgcatctt gttcgcgccg atcggcggct tgttgaggtc ggtcgcgtac
     1021 cagtagagcg gcaggcggcg ggcaaagtac cagcgcccgt cattgcggac atagtcatcg
     1081 acatacatca tctgcatgat cacccattca tcgccggttt catgctcgtt cttggaatag
     1141 acgatcccgt gggcgtggtc ggcatcgtcg aattcgatta catggccacc gatgtggtgg
     1201 ctggtgccgg taaaggggct gcgcagcgtg cggtccatgt aggcgcgcag ggcctgccgg
     1261 ccgctggcat ccttgcccac ccggacatcg gcgggaaaca gcgaaaccat tgcctccatg
     1321 tcgcgcatgt cgagtgcgag ggcatacttg gcgggaagct ggcggatcgc gtcgagcgat
     1381 tcgagccggt cgatcctggc gagcaaggca ctgtcggtca tggttcaatc ctttcgaacg
     1441 gagggaggac atagcgtccg tcgagagctt cggcgcgcgg caggtagaga cgggcgacca
     1501 gggtgaagga cccgggcggc accggcaacc agtttgccgg gcctgagcgc gggcgactgg
     1561 cggacagcgt cagggtcaac gatccatcgg cggcatattt gagtcctttg gtccggctgc
     1621 cgatcgaata gcggcgcagc gcgttctcgg tcagctgcat cccgggcgcg gcatagggcg
     1681 tgatcgacca gaatgcatcg accggcggca gctggccctt ggggaaacgg atccggtagg
     1741 cagacgcgcc attgagtggg cggcgctggc tgtccatcac cgcggccggg tagatcgatt
     1801 cctcgcgcgc gttaacatag ccgccgcgca ccacttcagc ccgcagtagg taatcgcttc
     1861 cgggattgct gaggtcgcgg gtctggagcc agccggtggc ggtgaagctg ggcttgaatg
     1921 cagtcttggc caacacctgc gggccgatcc gcaccgcgca gcccagacca agcgcttgcg
     1981 caggttccag cttgtccgga tcgaaagtgg ctgaggggcc aaatccggcg cggtcgaact
     2041 gggccatcag cgcttcctca ccagcttggc gcggcagtcc cttcagcgcc tggttgagca
     2101 cgataaagta ctcgaggctg gccaccgggt tcagcggttc ggcattggta accggttcgc
     2161 ctggcttgcc gatcatccgg atcgattggg ccaggcgttt tgcctcggct tcatcggcgc
     2221 ggccttcggc catgatccgg cccagcatcc aggccgtatc ggttggcaag ctgaaggctt
     2281 cgtagccatc gggaacattg ccgcccgatg gccccagcag ggcatagcgc cgcgcctcac
     2341 cgcggttggt ccgcgtgccc aggtgatgcg gggcggaata gaagtccatg aaggccagcg
     2401 tgtaatagcg cgaacccatc gccg
//
LOCUS       DASUDV010000528         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_526213,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000528 DASUDV010000000
VERSION     DASUDV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526213"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFV30_11575"
     CDS             <1..864
                     /locus_tag="VFV30_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4848777.1"
                     /translation="AATPPPAPPAADKGKPRDDKGGKRGKGGKDTPAAAPVRTAAAVP
                     PPEPQVGRETSTPAPQLAQAAPAPKPTPRPTPPPPPPPPTPAPAPLLPSPTPPAPTPG
                     FTTVAQGPGFDLAKAPAATPVRSDPPKPAPAPKPAPSPSIDEAFADLAPPSREVEPQA
                     GAVDIRRVRPAAPPAQATTKGKDPAKDGKPAAPSHPSRIWVQLATGRDKSALGFDWRK
                     MAKDNPALFKGLRPFVTAWGQSNRLLAGPFDTAKEANAFLAAAKKAGISGGFVWTSPA
                     GQIVDALGGGK"
     gene            976..1479
                     /locus_tag="VFV30_11580"
     CDS             976..1479
                     /locus_tag="VFV30_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX4848778.1"
                     /translation="MQSDPHYERDEDAAPVDMLASLFEARGWPCEFVGEDEISGEIKG
                     SWATYTLRGVWRREDHVLQLLCLPDIRVPDDKRASMFELLALVNEQLWVGHFDMWSNG
                     SVLLYRHGLLLGDEGLLSLAQAQVAVEAAVEECDRFYPAFQFVLWGDKSPAEALASSM
                     VDAAGEA"
     gene            1546..2343
                     /gene="proC"
                     /locus_tag="VFV30_11585"
     CDS             1546..2343
                     /gene="proC"
                     /locus_tag="VFV30_11585"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX4848779.1"
                     /translation="MTDTSILICGCGNMGGAMLAGWLAAGLDPARFTVVDPVLPSAPA
                     GVRLLREVPAGPFGTVLLGIKPQALPDVAPGLAPATRGAVVLSILAGAQLDTLAAAFP
                     DAAAWVRVMPNLAAAIGKSPLGLYAQGLDPDGRAAIEALLAPLGTAEWLAGEDQLDAV
                     TALAGSGPAFVYRFIAALAEGGAELGLDPAQAERLARAMVEGAAALAAASDASPEELA
                     RRVTSPGGTTAAGLAVLDAEGLFARRIGETLRAARDRGAELAAAAKA"
     gene            2454..3197
                     /locus_tag="VFV30_11590"
     CDS             2454..3197
                     /locus_tag="VFV30_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX4848780.1"
                     /translation="MSDWNDPRTTQSPFGSGPAINGASLETVTMDQGLRRYMLSIYNY
                     MASGVLLSGIVALLFANSGFAAQVMGTPLRWVIALAPLAFVMVMSFGMHRLSTATLNL
                     LFWAFSVVMGLSLSAIFLIYTGPSIALTFFSTAGAFAGLSLYGYTTKRNLSGMGSFMM
                     MGLIGLILASLLNLWLQSGPLSLAISAIGVIIFAGLTAWDTQRLRETYYHVAGTDMMG
                     KVVIMGALSLYLDFINMFQFLLSFLGNRE"
     gene            3343..4065
                     /locus_tag="VFV30_11595"
     CDS             3343..4065
                     /locus_tag="VFV30_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848781.1"
                     /translation="MGSHLRKTAALITVLAGVAGLAACGPKSTPPPPPPPPPPPPPPV
                     VYIPPRPLPPLGAASGVVIPPAGPDGTRKTINTGITPAQTIWNLRSAYNVAALNCLKP
                     QHADILVGYKKFLVTHKTGLATANRTVDTEFKKRYGTGYIRPREAYMTQVYNYYAFPP
                     TLNNFCDAALIMARESMTVKPADLGAFAKRSLVAFDGVFEGFYRAYEQYRFDADAWDA
                     KYAPKPAAPAPTGAAGPAPSGS"
     gene            4160..4235
                     /locus_tag="VFV30_11600"
     tRNA            4160..4235
                     /locus_tag="VFV30_11600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4193..4195,aa:Ala,seq:cgc)
     gene            4499..>5236
                     /gene="obgE"
                     /locus_tag="VFV30_11605"
     CDS             4499..>5236
                     /gene="obgE"
                     /locus_tag="VFV30_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX4848782.1"
                     /translation="MHFLDQAKIYIRSGQGGPGAVSFRREKYVEYGGPDGGNGGKGGD
                     IIFEAVAGLNTLIDFRYAQHFKAPRGVHGSGKNRNGAAGKDLVIKVPVGTQLISDEDR
                     ETVLLDLTEAGQRVTFLHGGLGGRGNASYKTSTNRAPRQHQPGEMGEEMYVWLRLKLL
                     ADAGLVGLPNAGKSTFINQITNTKAKVGDYAFTTLTPQLGVVRHRNREFVLADIPGLI
                     EGAAEGAGIGDRFLGHIERCRVLIHLID"
BASE COUNT          810 a         1872 c         1720 g          834 t
ORIGIN      
        1 gccgcaacgc cgccgccggc acccccggcc gccgacaagg gcaagccccg cgacgacaag
       61 ggcggcaagc gcggcaaggg cggcaaggat accccggctg cggccccggt ccgcaccgcc
      121 gccgccgtgc cgccgcccga accgcaggtc ggccgggaaa ccagcacgcc cgcgccgcag
      181 ctggcccagg ccgcaccggc acccaagcca accccgcggc ccacgccgcc gccgccaccg
      241 ccaccgccga cgcccgctcc ggcaccgctg ttgccttcgc ccacgccgcc cgcgccgacc
      301 ccgggcttta ccaccgttgc gcagggcccc ggcttcgatc tggccaaggc cccggcggcc
      361 actccggtcc ggtccgatcc gcccaagccg gctccggcgc ccaagcctgc accgtcaccg
      421 agcatcgacg aagcctttgc cgatctcgcc ccgccgagcc gcgaggttga gccgcaggcc
      481 ggcgcggtcg atatccgccg ggtccgtccc gccgctccgc ccgcccaggc cacaaccaag
      541 ggcaaggatc cggccaagga tggcaagccc gccgcgccca gccatcccag ccggatctgg
      601 gttcagctgg cgaccgggcg cgacaagtcc gcgctgggct ttgactggcg caagatggcc
      661 aaggacaatc ccgccctgtt caagggtctg cggcccttcg tgaccgcctg ggggcaaagc
      721 aaccgcctgc tggccgggcc gttcgatacc gccaaggagg ccaacgcctt tctggccgcg
      781 gcgaagaagg ccgggatttc gggtggtttc gtctggacca gcccggccgg gcagatcgtc
      841 gacgcgctgg gcgggggcaa gtaggggcga tccacccctt ttccacacct tgtaaacagg
      901 gttgtcgagt tttgcgcagg cctgccgggc gcgcggattg tcgggcaccc tttgccgctg
      961 catccagcgc ccatgatgca aagcgatccg cattacgagc gcgacgagga cgctgccccc
     1021 gtcgatatgc tcgcctccct gttcgaggcg cgcggctggc cttgcgaatt cgtcggcgag
     1081 gacgagatct cgggcgagat caaggggtcc tgggccacct acaccctgcg cggggtctgg
     1141 cggcgcgagg atcacgtcct gcagctgctg tgcctgcccg atatccgcgt gccggacgac
     1201 aagcgcgcca gcatgttcga actgctggcc ctggtgaacg aacagctgtg ggttggccat
     1261 ttcgacatgt ggtccaacgg cagcgtcctg ctctaccgcc acggcctgct gctgggggac
     1321 gagggtctgc tcagcctggc ccaggcccag gtcgcggtcg aggccgcggt ggaagagtgc
     1381 gaccggttct atcccgcctt ccagttcgtg ctgtggggcg acaagagccc ggccgaggcg
     1441 ctggccagtt cgatggtcga cgccgccggc gaggcctgag caacaacccg ctggacattc
     1501 gccgcggcgg ggctagccac cgggtgatcc tttgcgggga ccacgatgac cgacacttcg
     1561 atcctgatct gcggctgcgg caacatgggc ggggccatgc tggccggatg gctggccgcc
     1621 gggctggacc cggcccgctt caccgtggtc gatccggttc tgcccagcgc gccggccggg
     1681 gtccggctgc tgcgcgaggt tccggccggg ccgttcggca cggtcctgct gggcatcaag
     1741 ccgcaagccc tgccggatgt ggcgcctggg ctggccccgg ccacgcgcgg ggcggtggtg
     1801 ctgtcgatcc tggccggggc gcagctggac acgctggcgg cggcctttcc ggatgcggcc
     1861 gcctgggttc gggtgatgcc caacctggcc gccgcgatcg gcaagtcgcc gctggggctc
     1921 tatgctcagg gcctcgatcc agacggccgc gcggcgatcg aggccctgct ggccccgctc
     1981 ggcacggcgg aatggctggc gggcgaagac cagcttgacg cggtgaccgc gctcgccggc
     2041 tccggtccgg ccttcgtcta ccggttcatc gccgccctgg ccgagggcgg ggcagagctg
     2101 ggcctcgatc cggcccaggc cgagcggctc gcccgggcga tggtagaagg ggccgcggcg
     2161 ctggccgctg cgtcggacgc cagcccggaa gagcttgccc ggcgcgtcac cagcccgggc
     2221 ggaacgaccg ccgcggggct ggccgtgctt gatgcagagg gcctgttcgc ccggcggatc
     2281 ggcgaaaccc tgcgcgccgc gcgcgaccgc ggggcagagc tggcggcggc ggcaaaggcc
     2341 tgacaccgtt cgtcgaaatt ggacgaaact tgtccggttt tgcttgaatc ctttaacgac
     2401 tcacacgata ttccgggcat cgctcctatc tcggagcggc aaaggagttt gtcatgtctg
     2461 attggaacga tcctcggacc acccaatccc cgttcggcag cgggccggcg atcaatggcg
     2521 catcgcttga aacggtcacc atggaccagg gtctgcggcg ctacatgctg tcgatctaca
     2581 actacatggc ctcgggcgtg ctgctttcgg gcatcgtcgc gctgctgttc gccaattcgg
     2641 gcttcgcggc ccaggtgatg ggcaccccgc tgcgctgggt gatcgcgctc gccccgctgg
     2701 ccttcgtcat ggtgatgagc ttcggcatgc accgcctgtc caccgcgacc ctgaacctgc
     2761 tgttctgggc cttcagcgtg gtgatgggcc tgtcgctgtc ggcgatcttc ctgatctaca
     2821 ccgggccgtc gatcgcgctg accttcttct ccaccgccgg cgctttcgcg ggtctcagcc
     2881 tctatggcta caccacgaag cgcaacctca gcggcatggg cagcttcatg atgatgggcc
     2941 tgatcggcct gatcctcgcc tcgctgctca acctgtggct gcagtcgggt ccgctcagcc
     3001 tggcgatctc ggcgatcggc gtgatcatct tcgccggcct gaccgcctgg gatacgcagc
     3061 gcctgcgcga aacctactac cacgttgccg ggaccgacat gatgggcaag gtcgtgatca
     3121 tgggcgcgct gtcgctctat ctcgacttca tcaacatgtt ccagttcctg ctgagcttcc
     3181 tcggcaaccg cgaatagccc tgcaaccgca attgcgggca tccatcgtgc ccggcgcgct
     3241 ttccggcgcg ccgggcattt gcattatcgg gccaagcggc taggaaccct ggccaaggat
     3301 gatccgatcg ccccggcggc ggatcgataa agggacggga atatgggttc gcacctccgc
     3361 aagactgccg ctctgatcac cgtgctggcc ggtgtggctg gcctcgccgc ctgcggcccg
     3421 aaatcgaccc cgccgccgcc tccgcccccg ccgcctccgc cgcccccgcc ggtggtctat
     3481 atcccgccgc ggccgctgcc gccgctgggc gcggcttcgg gggtggtcat cccgccggcc
     3541 ggtccggacg ggacccgcaa gacgatcaac accgggatca ccccggcgca gacgatctgg
     3601 aacctgcgct cggcctacaa cgtggccgcg ctcaactgcc tcaagccgca gcacgcggac
     3661 atccttgtcg gctacaagaa gttcctggtg acccacaaga ccggcctggc aacggccaac
     3721 cgcacggtcg atacggagtt caagaagcgc tacggcaccg gctatatccg cccgcgcgag
     3781 gcctacatga cgcaggtcta caactattac gccttcccgc cgacgctgaa caacttctgc
     3841 gatgcggcgc tgatcatggc ccgcgaatcg atgacggtga agccggccga tctgggcgct
     3901 ttcgccaagc gcagcctggt ggctttcgac ggcgtattcg aaggcttcta ccgcgcttac
     3961 gagcagtacc gcttcgacgc cgacgcctgg gacgccaagt atgcgcccaa gcccgctgca
     4021 ccggcgccga ccggcgcggc cggcccggcg ccgtcgggca gctgaccggc gaaattagcc
     4081 cttccaagcg gacaaggctt tcgctaaggg ggcgcctcgc gcgtgcagac cgtttccgca
     4141 cgcacgctcg cactggaacg gggccgtagc tcagatggga gagcgcgtcg ttcgcaatga
     4201 cgaggtcagg ggttcgatcc ccctcggctc caccagtgtg ctgataaccg ctccatcttg
     4261 gcctgcgaac ggcaccggac tgcgcttccg gtgctcacga cccacaaggt cgctgcgcgc
     4321 cggttctcgt ccggcaccat tctcggcaca agctgaagcg gttctcagca cactggtcca
     4381 aaccctcagc gccgttcgtg tcgagccctt cggcttgcgg cttagcagcc gctcaggata
     4441 aaccaaggcc gagacaccat gcgcgacttt tacctcctga cccgttagaa ccaatcccat
     4501 gcattttctc gaccaggcca agatctacat ccgctcgggc cagggcggcc ccggggccgt
     4561 gtcgttccgg cgggaaaagt acgtggaata tggggggccc gatggcggca acggcggcaa
     4621 gggcggcgac atcattttcg aagccgtggc cgggctcaac accctgatcg acttccgcta
     4681 tgcccagcac ttcaaggccc cgcgcggggt tcacggttcg ggcaagaacc gcaacggcgc
     4741 ggccggcaag gatctggtga tcaaggttcc ggtcggcacc cagctgattt cggacgagga
     4801 ccgcgaaacc gtgctgctcg atctgaccga ggccggccag cgcgtcacct tcctgcacgg
     4861 cgggctcggc gggcgcggca acgcctccta caagaccagc accaaccgcg ccccgcgcca
     4921 gcaccagccg ggcgagatgg gcgaggaaat gtacgtctgg ctgcggttga agctgctggc
     4981 cgacgcgggc ctggtcggcc tgcccaacgc tggcaagagc accttcatca accagatcac
     5041 caacaccaag gccaaggtcg gcgattacgc ctttaccacg ctgaccccgc agctgggcgt
     5101 ggtccgccac cgcaaccgcg aattcgtgct ggccgacatc cccggcctga tcgagggcgc
     5161 ggccgaaggg gccgggatcg gtgaccgctt cctgggccat atcgagcgtt gccgggtgct
     5221 gatccacctg atcgac
//
LOCUS       DASUDV010000529         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1630841,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000529 DASUDV010000000
VERSION     DASUDV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630841"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFV30_11610"
     CDS             <1..338
                     /locus_tag="VFV30_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848783.1"
                     /translation="KPANALDGRELECVAALLLFETFLAEQIKNKGPTTPEGLDYTRA
                     AKTNAAALGWYLGRVSSMPADRVGKSAFVEANAKVRGMEAKKALVFIQDCTQNYQENA
                     GEAFGAWLG"
     gene            445..927
                     /locus_tag="VFV30_11615"
     CDS             445..927
                     /locus_tag="VFV30_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848784.1"
                     /translation="MFRSSIAALSLASLALAAVPAAAQTAPASAAPMPKVAQPSDLGC
                     VLRMMDLSKMATGNANNAARTEEQRANSMTMAHDARRTLYYFVGKMGPAYSTKNRNAE
                     GEAEFNKMRATGDKELAAEMAMCMREAAGAERALLEAMRAPAAATPAAPANSGKPARN
                     "
     gene            965..1534
                     /gene="pth"
                     /locus_tag="VFV30_11620"
     CDS             965..1534
                     /gene="pth"
                     /locus_tag="VFV30_11620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX4848785.1"
                     /translation="MQIWVGLGNPGPQYALHRHNVGFIVCDTLAEVHGFGPVQKKFLG
                     WVQEGRIGGEKILLLKPATFMNESGRAVGEALRFYKLGTEALTVFHDELDLAPFKVKV
                     KRGGGTAGHNGLRSIDQHLGPDFRRVRIGIGHPGHKDRVTGYVLGNYAKAEMDPLADM
                     LGSIAAEAEWLAKDDDARFMSEFALRMAE"
     gene            1606..>2734
                     /gene="purB"
                     /locus_tag="VFV30_11625"
     CDS             1606..>2734
                     /gene="purB"
                     /locus_tag="VFV30_11625"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX4848786.1"
                     /translation="MVPRYARPAMVAIWEPEARYRIWFEIEAHATEKLGELGVVPPSG
                     AKALWDWWATNPAIDVAAIDAIEAVTKHDVIAFLDWVAQQVGPEARFMHQGMTSSDVL
                     DTTLAVQLARASDLLLEDLDALLAAIKRRALEHKYTPTIGRSHGIHAEPTTFGKKLAE
                     AYAEFSRCKTRLLAAREEIATCAISGAVGTFANIDPSVEEYVAEKLGLKPEPVSTQVI
                     PRDRHAMFFATLGVIASSIERLAVEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPV
                     LTENLTGLARMVRSAVTPALENVALWHERDISHSSVERFIGPDATITLDFALARLTSV
                     VDKLLVYPERMQKNLDKMGGLVHSQRVLLALTQAGVSREDSY"
BASE COUNT          498 a          934 c          869 g          433 t
ORIGIN      
        1 taaagcccgc aaacgcactg gacggccgcg agctggaatg cgtggccgcg ttgctcctgt
       61 tcgaaacgtt tcttgccgag cagataaaga acaagggtcc gaccacgccg gaaggtctgg
      121 attacacgcg tgccgccaag accaacgccg ccgcgcttgg ctggtatctc ggccgtgttt
      181 ccagcatgcc tgccgatcgt gtgggcaagt ccgcttttgt ggaagcaaac gccaaggtgc
      241 gcgggatgga agccaaaaag gccctcgtct tcatccagga ctgcacccag aactatcagg
      301 aaaatgccgg ggaggccttc ggcgcctggc ttggctaagc cgccgttggc acgccgccgg
      361 ttgaccatcc ggggcgttgt aagccaaggt tcagccatgc cctgccacag cgcgggaatg
      421 tttcttctta agaggggttt gcccatgttc cgttcgtcga ttgccgccct gtcgctggcc
      481 agcctggcgc ttgccgccgt tccggccgct gcccagaccg cgcccgcttc tgccgcgccg
      541 atgcccaagg tggcccagcc cagcgatctt ggctgcgtgc tgcgaatgat ggacctgtcc
      601 aagatggcga ccggcaatgc caacaatgcc gcccggaccg aagagcagcg cgccaattcg
      661 atgaccatgg cccacgatgc ccgccgcacc ctgtattact tcgtcggcaa gatgggcccg
      721 gcctattcca ccaagaaccg caacgcggaa ggcgaggcgg agttcaacaa gatgcgcgcc
      781 accggcgaca aggaactggc cgccgaaatg gcgatgtgca tgcgcgaggc cgccggggcg
      841 gagcgcgcgc tgctcgaagc catgcgcgct ccggcagccg ccacgcctgc agctcccgcc
      901 aactcgggca agcccgcccg caactgacgc gccccgaccg gcccggaaca aggaatccaa
      961 acccgtgcaa atctgggtcg gtctcggcaa ccccggtccg cagtacgcgc tgcaccggca
     1021 caatgtcggc ttcatcgtct gtgataccct ggccgaagtg cacggcttcg gcccggtcca
     1081 gaagaagttc ctcggctggg tccaagaagg gcggatcggc ggggaaaaga tcctgctgct
     1141 caagcctgcg accttcatga acgaaagcgg ccgcgcggtg ggcgaggcgc tccgcttcta
     1201 caagctgggc accgaggccc tgaccgtgtt ccacgacgag ctggacctgg cccccttcaa
     1261 ggtcaaggtg aagcgcggcg gcggcacggc cgggcacaat ggcctgcgct cgatcgacca
     1321 gcaccttggc cccgatttcc gccgggtgcg gatcgggatc ggccaccccg gccacaagga
     1381 ccgggtgacc ggctatgtcc tcggcaacta tgccaaggcg gaaatggacc cgctggccga
     1441 catgctcggc tcgatcgcgg ccgaagccga atggctcgcc aaggacgacg acgcccgctt
     1501 catgagcgaa ttcgcgctgc ggatggcgga gtagcgccgc cctccccctt gccttgacgg
     1561 cgctggcccc ctagcggctc cttcgaaccc caaaaggagt cgatcatggt cccccgctac
     1621 gcccgccccg caatggtcgc gatctgggag ccggaggcgc gctatcgcat ctggttcgag
     1681 atcgaggccc acgccaccga gaagctgggc gaactgggcg tggttccgcc gagcggcgcc
     1741 aaggcgctgt gggactggtg ggcgacgaac cccgccatcg atgtcgccgc gatcgacgcg
     1801 atcgaagccg tcaccaagca cgacgtgatc gccttcctcg attgggtggc ccagcaggtc
     1861 ggcccggaag cgcgcttcat gcaccagggc atgaccagct ccgacgtgct ggataccacg
     1921 ctggccgtcc agctggcccg cgccagcgac ctgctgctgg aagacctcga cgcgctgctc
     1981 gccgcgatca agcgccgtgc gctggagcac aagtacaccc ccacgatcgg ccgcagccac
     2041 ggcatccatg ccgagccgac caccttcggc aagaagctgg ccgaggctta tgccgaattc
     2101 agccgctgca agacccgcct gctcgccgcg cgcgaggaaa tcgccacttg cgccatttcc
     2161 ggcgcggtcg gcactttcgc caacatcgat ccgtcggtgg aggaatatgt cgccgagaag
     2221 ctggggctga agccggaacc ggtcagcacc caggtgatcc cgcgcgatcg ccatgcgatg
     2281 ttctttgcca cgctgggcgt gatcgccagt tcgatcgagc ggctcgcggt tgaaatccgc
     2341 cacctccagc gcaccgaagt gctcgaggcg gaagaatact tctcgcccgg ccaaaagggc
     2401 tcgtcggcca tgccgcacaa gcgcaacccg gtgctgaccg agaacctgac gggcctggcc
     2461 cgcatggtcc gctcggccgt caccccggcg ctcgagaatg tggcgctgtg gcacgagcgc
     2521 gatatctcac actcttcggt tgagcgtttc atcggtccgg acgccaccat cacgctcgat
     2581 ttcgcgctgg cccggctgac ctcggtggtc gacaagctgc tggtctatcc ggagcggatg
     2641 cagaagaatt tggacaagat gggcggcctc gtccattcgc agcgcgtgct gctggctctg
     2701 acacaggcgg gcgtgagccg cgaagacagc tatc
//
LOCUS       DASUDV010000530         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2138788,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000530 DASUDV010000000
VERSION     DASUDV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138788"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1155
                     /locus_tag="VFV30_11630"
     CDS             181..1155
                     /locus_tag="VFV30_11630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4848787.1"
                     /translation="MGNGLPARRFEMLDAYRGIAALAVLFYHAGAQAPLLAPRGYLGA
                     DLFFILSGFVLAHAYGARLRGGLGLAGFIRLRLIRIYPMFLMSAVLGALLFHGSPLML
                     LMLPTPSADGLLFHANAPLWSVTFELLISLGYCLLAPRLGLRGLLGIAAVTGLVLARG
                     ILTHDGADLGAFAATAGLGLIRTAYDFTIGVLLHRAYLARLRAPVTGTIGLALPVVLV
                     LVLLVDPAGRAWWDIATIGALFPALVWLGACTALPRPALGERLGGISYPLYCIHAPIV
                     AMCHASARLMGLASLALVVAAWALNRWFDRPLQRLARRDPSPGRIASA"
     gene            1241..>2168
                     /locus_tag="VFV30_11635"
     CDS             1241..>2168
                     /locus_tag="VFV30_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4848788.1"
                     /translation="MPSYDLIIRNGTIVDGTGAARFTGDVAVKDGLIAAVGTVHGDAT
                     REIDAAGKIVAPGWVDVHTHYDGQATWDQEMAPSSWHGVTTVVMGNCGVGFAPAAPNR
                     HEWLIGLMEGVEDIPGTALAEGMKWDWETFPEYLDALERLPRTVDVGTHVPHGAVRAY
                     VLGDRERPGAVPTAEEIAEMAKIVEDGVRAGALGFSTSRTVLHRSIDGEVVPGTTATA
                     EELVEIGRAMGRAGHGVFEMASDMMREWNEFGWMGQLSRETGLPVTFAALQSIAKELP
                     LEEQIEKMRAENDNGANIVAQIALRGNGIVMAW"
BASE COUNT          326 a          701 c          733 g          408 t
ORIGIN      
        1 accttccgcg actggaagaa gatcgaagcc gaagaggaag cccgcgcccg caccggcgcg
       61 ccgcgcgaaa agttcgtcga tgtcgatgcc atgatcaagg cgcgcggcga ctgaacctgc
      121 tgtcagcaag tctgcgccaa acggggtttg ttaatcgaaa tcggctaggt cgctttcctc
      181 atgggtaacg gccttccagc gcggcgcttc gaaatgctcg atgcctatcg cggcatcgcg
      241 gcgctggcgg tactgttcta ccatgccggc gcccaggctc cgctgctggc gccgcgcggc
      301 tatctgggcg cggatctgtt cttcatcctt tcgggattcg tgctggccca tgcctatggc
      361 gcacggctgc gcggcgggct gggactggcc gggttcatcc ggctgcggct gatccggatc
      421 tatccgatgt tcctcatgag tgcggtgctg ggcgccctgc tgttccacgg cagcccgctg
      481 atgctgctga tgctgcccac gccttcggcc gatggcctgc tgttccatgc caatgctccg
      541 ctgtggtcgg tcactttcga actgctgatc agcctcggct attgcctgct ggcgccaagg
      601 ctggggctgc gcgggctgct gggcatcgcc gccgtgaccg ggctggtcct ggcgcgcggg
      661 atcctgactc atgacggtgc ggaccttgga gcctttgccg ccacggccgg gctgggcctg
      721 atccgcaccg cctatgattt caccatcggc gtgctgttgc accgcgctta cctggccagg
      781 cttcgtgcgc ccgtgaccgg cacgatcggc ctggccttgc cggttgtcct ggtgcttgtc
      841 ctgctggtcg atccggccgg gcgggcgtgg tgggacattg ccaccatcgg cgcactcttt
      901 cccgcgctgg tctggcttgg cgcctgcacc gccctgcccc gacccgcgct gggcgagcgg
      961 ctgggtggga tttcctatcc gctctattgc atccacgcac cgatcgtggc gatgtgccat
     1021 gcctcggcca gattgatggg cctggcatcc ctcgccctgg tggtggcagc ctgggcgctg
     1081 aaccgctggt tcgaccgacc gctacaacgg ctggcccggc gtgatccttc gcctggccgg
     1141 attgcctctg catagggctt gccccatccc cgaagctgtg tcttaatcgc ccggttaagc
     1201 gcgccctgac cagagtcggc gcgatccggg agagtgagcc atgcccagtt acgacctgat
     1261 catccgcaat ggaaccatcg tcgatggcac cggcgcagcg cggtttaccg gcgatgttgc
     1321 ggtgaaggac gggctgattg cggcggttgg cacggtgcac ggcgatgcca cgcgcgaaat
     1381 cgatgccgcg ggcaagatcg tcgctcccgg ctgggtcgat gtccacaccc attacgatgg
     1441 ccaggccacc tgggatcagg aaatggcccc gtcgagctgg cacggggtga ccacggtggt
     1501 gatgggcaat tgcggcgtgg gctttgcccc tgccgcgccc aaccggcatg agtggctgat
     1561 cgggctgatg gaaggggttg aggatattcc cggcaccgcg ctcgccgaag ggatgaagtg
     1621 ggactgggaa accttccccg aatatctcga tgcgctcgaa cgcctgccgc gcacggtcga
     1681 tgtcggcacc catgtgcctc acggcgccgt gcgggcctat gtcctgggtg accgcgagcg
     1741 accgggcgca gtgcccaccg cagaggaaat cgccgagatg gcgaagatcg tcgaggacgg
     1801 tgtgcgggcc ggcgccctcg gcttttccac ttcgcgcacc gtgcttcacc gctcgatcga
     1861 cggcgaggtc gttccgggca ctacggccac tgccgaggag ctggtcgaga tcggccgcgc
     1921 aatgggccgc gcgggccacg gcgtattcga aatggccagc gatatgatgc gcgaatggaa
     1981 cgagttcggg tggatgggcc agctcagccg cgagaccggc ctgcccgtca cattcgccgc
     2041 gctccagtcg atcgcgaagg aactcccgct cgaagagcag atcgagaaga tgcgcgccga
     2101 aaatgacaac ggcgccaaca tagtcgccca gatcgcgctg cgcggaaacg gcatcgtgat
     2161 ggcatggc
//
LOCUS       DASUDV010000531         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1652930,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000531 DASUDV010000000
VERSION     DASUDV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652930"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VFV30_11640"
     CDS             complement(<1..1661)
                     /locus_tag="VFV30_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4848789.1"
                     /translation="MPALKSPLDWFLSGSIARKTNRLLGIPTAVSLLIGTFVIVIGFL
                     GVQTVNRSADMRLANHHASVIEDESANIVLRARIEGDRIAAGSDARLTEGVKRAEEAA
                     KALEGDLAALGFGQSDLAALQQRMSFLSSAAQQGGISAQDLTFKALELQEKARKVRGT
                     IDTQAAVMVDSNVSRIYRAVSIGALLMLLALITFFAARRATRTMLVEPIVATRDGLIS
                     LTGGSQDFDVAGIDRDDEIGDMARALEGLKDYSRQFVELHTKKEERARLREEQVQARE
                     AERQQRAELLRELALRFEGTVGGVVGGVAAASTQLQATASSMAAAAEQSSLQTSAVTG
                     SLGEASVGVTAAASACDEFAMSISEVSRQAASSAELARQATASAANADQAIATLTLSA
                     AQIGEIVELISAIAQRTNLLALNASIEAARGGEAGRGFAVVAAEVKDLAMQTARATEE
                     VAEQIRAMQDSTEVSTDALRDIVKQIQALEHTSISIAAAVDEQAVASQDLARSIDVAA
                     RNTAEVSTNIVQVRETALATGTAAQQVLSSSQELQSQADVLRTQV"
     gene            1797..3041
                     /locus_tag="VFV30_11645"
     CDS             1797..3041
                     /locus_tag="VFV30_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX4848790.1"
                     /translation="MDLRLSRRRFGLGAAAAAALPGAAFAAKAEPFQTGLDRLVAGEG
                     WLARGKSVALLTHAGAVDAKGRRAADAIAAMAEPDLVALLAPEHGLAGQLPAGADVPD
                     GRDPATGLPVYSLYGRTTLPTELLARIDTILIDLQDVGVRPFTYAWTMRTALRIAAEH
                     GKRALVLDRPNPLGGRKVDGPMVDPALASPVGATPVPFRHGLTMGEMAGLIQADENLM
                     VDLSVMPMKGWTRDRGIGVYGRGGLPFVAPSPNLRSPGAILAYAAAVLVEGTNLSEGR
                     GTPRPFETIGAPWCDGKALARTLTDLDLPGVRFKPVTFTPTTSKHSGQPCAGVRFEVR
                     DEAAYDPLLTGLSLIAAARARHPDRFAFLPGERPFFDLLTGQAWVRQALLDGDSPAAI
                     VRRWQTRLPAFRRLTRAFALYD"
     gene            complement(3048..>3305)
                     /locus_tag="VFV30_11650"
     CDS             complement(3048..>3305)
                     /locus_tag="VFV30_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848791.1"
                     /translation="PPVAEAGPDDLSRIKGLGPKLQALLPTLGITTFAQIAALSDADL
                     ADLDGKLGPFAGRPAKDNWVEQAKFLAAGDVAGFEAKFGKV"
BASE COUNT          459 a         1154 c         1158 g          534 t
ORIGIN      
        1 tccacctggg tgcgcagcac gtcggcctgg gattgcagct cttgcgagga cgacagcacc
       61 tgctgggccg ccgtgccggt cgccagcgcg gtttcgcgga cctggacgat gttggtgctg
      121 acctcggcgg tgttgcgggc ggccacgtcg atgctgcggg ccaagtcctg gctggcgacg
      181 gcctgttcgt cgaccgccgc ggcgatcgag atcgaggtgt gttccagcgc ctggatctgc
      241 ttgacgatgt cgcgcagcgc atcggtgctg acttcggttg aatcctgcat cgcccggatc
      301 tgctcggcga cctcttcggt ggcccgggcg gtctgcatcg ccagatcctt cacttcggcc
      361 gcaaccacgg caaagccgcg cccggcctcg cccccgcgcg ccgcctcgat cgaggcgttg
      421 agcgccaaga ggttggtgcg ctgggcaatg gcggaaatca gctcgacaat ttcgccgatc
      481 tgcgcggcgg agagcgtcag cgtcgcgatc gcctgatcgg cattggcggc gctggccgtg
      541 gcctggcgcg cgagttcggc ggacgaggcc gcctgacggc tgacctcgct gatcgacatc
      601 gcgaattcat cgcaggccga ggccgccgcc gtcaccccga ccgaggcttc gccaaggctg
      661 ccggtgaccg cgctggtctg gagcgaggac tgttcggccg cagccgccat cgacgaggcg
      721 gtggcctgca actgggtcga cgccgccgcc accccgccaa ccacgccgcc gacggtgcct
      781 tcgaaccgca gcgccagttc gcgcagcagt tcggcgcgct gctggcgctc ggcctcgcgg
      841 gcctggacct gctcttcgcg caggcgggcg cgttcttcct tcttggtgtg gagttcgacg
      901 aactggcggg aatagtcctt cagcccttcg agcgcgcggg ccatgtcacc gatctcgtca
      961 tcccggtcga tcccggcaac gtcgaagtcc tggctgccgc cggtcaggct gatcaggccg
     1021 tcgcgggtgg cgacgatcgg ttcgaccagc atggtgcggg tggcgcggcg ggcggcaaag
     1081 aaggtgatca gcgcaagcag catcagcagc gctccgatcg acaccgcacg gtagatccgg
     1141 ctgacgttgg aatcgaccat caccgcggcc tgggtatcga tcgtcccgcg aaccttgcgg
     1201 gccttttcct gcagctccag cgccttgaag gtcaggtcct gcgccgagat gccgccctgc
     1261 tgggcggcgc tgctcaggaa ggacatgcgc tgttgcagcg cggccagatc ggactgcccg
     1321 aagcccaggg ccgccagatc gccttccagc gccttcgcgg cctcttcggc gcgcttcacg
     1381 ccttcggtca gccgcgcatc ggagccggcc gcgatgcgat cgccctcgat ccgggcgcgc
     1441 agcacgatgt tggcgctttc gtcctcgatc accgaggcgt gatggttggc cagccgcatg
     1501 tcggcagagc ggttgacggt ctgcacgcca aggaagccga tcacgatcac gaaagtgccg
     1561 atcagcagcg aaacggcggt gggaatgccc agcaagcggt tggtcttgcg ggcaatggag
     1621 cccgacagga accaatccag cggtgatttc agggctggca cggggcacac ctctctcagc
     1681 agacaagtgc ctgcaaacta ggccttgcat ggttaatttt gtgtcaagcg ggcgggccgg
     1741 tgcgcgggcg gcaatcgcgg cttgatcggc cgcgccgcgg cgttagcttg cggccgatgg
     1801 accttcgcct ttcccgccgc cgcttcggcc ttggcgccgc agccgccgcg gctctccccg
     1861 gcgcggcctt tgccgcgaag gcggaaccgt tccagaccgg gctggaccgg ctggtcgccg
     1921 gggaaggctg gctggcccgg ggcaagtccg tggccctgct gacccatgcc ggggccgtgg
     1981 acgcgaaagg gcggcgcgcg gccgatgcga tcgcggccat ggcagagccc gatctggtgg
     2041 cgctgctggc ccccgaacac ggcctggccg ggcagctgcc ggccggggcc gatgtgccgg
     2101 acgggcggga tcccgccacc gggctgccgg tctacagcct ttacggccgc accaccctgc
     2161 cgaccgagct gctggcgcgg atcgatacga tcctgatcga cctgcaggac gtgggcgtgc
     2221 ggcccttcac ctatgcctgg accatgcgca ccgcgctgcg gatcgcggcc gaacacggca
     2281 agcgggcgct ggtgctggac cggcccaacc cgctgggcgg gcgcaaggtg gacgggccga
     2341 tggtcgatcc cgcgctggcc tcgccggtcg gcgccacccc ggtgcccttt cgccacggcc
     2401 tgaccatggg ggaaatggcc ggcctgatcc aggcggacga gaacctgatg gtcgatctgt
     2461 cggtgatgcc gatgaagggc tggacccgcg atagggggat cggggtctat ggccggggcg
     2521 ggctgccctt cgtcgcgccc tcacccaacc tgcgcagccc cggcgcgatc ctggcctatg
     2581 ccgcggcggt gctggtcgag gggaccaacc tgtccgaagg gcgcggcacc ccgcgcccgt
     2641 tcgagacgat tggcgcgccg tggtgcgatg gcaaagcgct ggcccggact ctcaccgacc
     2701 ttgaccttcc cggagtgcgg ttcaagccgg tgaccttcac cccgaccacg tccaagcaca
     2761 gcggccagcc ctgcgccggg gtgcggttcg aagtgcgcga cgaggcggcc tatgatccgc
     2821 tgctgaccgg cctgtcgctg atcgccgcgg cgcgggcccg gcatccggac cgctttgcct
     2881 tcctgcccgg cgaacggccg ttctttgacc tgctgaccgg ccaggcctgg gtgcgccagg
     2941 ccctgctgga cggagacagc ccggcggcca tcgtccggcg ctggcaaacc cgcctacccg
     3001 ccttccgccg gttgacccgg gccttcgccc tctacgacta ggctggccta gaccttcccg
     3061 aatttcgcct cgaacccggc cacgtcgcct gcggcgagga acttggcctg ttcgacccag
     3121 ttgtccttgg ccggacgccc ggcaaagggc cccagcttgc catccagatc ggccagatcg
     3181 gcatcgctca gcgcggcgat ctgggcgaag gtggtgatcc ccagcgtcgg cagcagggcc
     3241 tgcagcttgg ggcccagacc cttgatccgc gacaggtcgt ccggcccggc ttcggcaacc
     3301 ggcgg
//
LOCUS       DASUDV010000532         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1608757,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000532 DASUDV010000000
VERSION     DASUDV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608757"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFV30_11655"
     CDS             complement(<1..678)
                     /locus_tag="VFV30_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4848792.1"
                     /translation="MADLFDNPLGLDGFEFVEFCAPEKGLLEPVFAGMGFTLIARHRS
                     KDVQLWRQGEINLIANYEPRSPAAYFAAEHGASACGMAFRVKDAAAAYKQAIERGAEP
                     VESKTGVMELRIPAIKGIGGAFIYLVDRYATLDNPDALSIYDIDFVYEPGVDRHPVGA
                     GFKKIDHLTHNVYGGRMAHWGAWYERIFNFREIRYFDIKGEYTGLTSRAMTAPDGKIR
                     IPLNEEGK"
     gene            complement(756..1898)
                     /locus_tag="VFV30_11660"
     CDS             complement(756..1898)
                     /locus_tag="VFV30_11660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4848793.1"
                     /translation="MRIVDVCAFYTPHGGGVKTYVEQKLRLGPELGHEIVILAPGDRY
                     EVIDRGPQARIVTIPSPRFPLDRNYWYFDDEDALHDALDSLRPDFVEASSPWRSPSMV
                     ARWKGSAPRSLFMHADPLSAYAYRVLEPIFSRETIDRRFEPFWQHLRRLGTEFDTVVC
                     ASGELRERLEEGGVANTALIPMGVEEGVFAPDRRDPALRRQLLELCGLPEEAGLLIGV
                     GRLSAEKRWPMVIDAVMAASTHAPVGLVLLGGGGQERKILKSIGANPHIRLLRPERNR
                     ARFAAILASADALVHGCEAETFCLSAAEARASGVPVIVPDRGGAADHAADGAGIAYTA
                     ADPEALAEAILTMAATGWPRTASAVQTMRGHFTELFAHYEALRRAG"
     gene            complement(1914..2456)
                     /locus_tag="VFV30_11665"
     CDS             complement(1914..2456)
                     /locus_tag="VFV30_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX4848794.1"
                     /translation="MKRVFLPMLLTVAVPLLLAAGPALRSSPDLGKAEGRCRPGEPGP
                     ALLVEAVGMKDRVGNMKLEVYPSNDTDFLADDNVLVAAGKVFRRVEVPTPASGPVVLC
                     VRIPGPGTYSVSVLHDRDSNRKFGWKIDGIGFSGNPKLGWSKPKAAKARVVVASGGVS
                     RIAVTMNYKNGLGVSPIKKK"
     gene            complement(2453..>3160)
                     /locus_tag="VFV30_11670"
     CDS             complement(2453..>3160)
                     /locus_tag="VFV30_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848795.1"
                     /translation="YLAGPVSEWFIYRRLWAMPIAGLGALIRKLVSNEILLGYLGEAQ
                     FYAWARARLNFVAAPFGAIKDVTILSALVGNFATLAMLAWAWPLIASGMLGLEGRTVF
                     ISLGVVLVTSFAILLFRQKLFSLAQRELWFISGVHLLRIAAVVILSAVMWHLVLPQVE
                     VALWIVLATLRMLVSRLPLLPNKDVVFAGIAVFLLGQDVEIAGLMTLMAVLLLITHLV
                     VGGLFALLDLFDAEKVK"
BASE COUNT          552 a         1080 c         1045 g          483 t
ORIGIN      
        1 cttgccttcc tcgttcagcg gaatgcggat cttgccgtcg ggcgcggtca tcgcccggct
       61 ggtcaggccg gtgtattcgc ccttgatgtc gaaatagcgg atctcgcgga agttgaagat
      121 ccgctcgtac cacgcgcccc aatgggccat gcgcccgccg tagacgttgt gggtcaggtg
      181 atcgatcttc ttgaagcccg cgccgaccgg atggcgatcg acgcccggtt cgtagacgaa
      241 gtcgatgtca tagatcgaaa gggcatcggg attgtcgagc gtggcatagc gatcgaccag
      301 atagatgaac gccccgccga tccccttgat cgccgggatg cgcagctcca tcaccccggt
      361 cttgctttcc accggctcgg ccccgcgttc gatcgcctgc ttgtaggcgg cggcggcatc
      421 cttcacgcgg aacgccatgc cgcaggccga agcgccgtgt tccgccgcga aataggccgc
      481 cgggctgcgc ggttcgtaat tggcgatcag gttgatttcg ccctggcgcc acagctgcac
      541 gtccttggac cggtggcggg cgatcagggt aaagcccatc cccgcgaaga ccggttccag
      601 caggcccttc tcgggggcgc agaactcgac gaattcgaag ccgtcgaggc ccaggggatt
      661 gtcgaacaga tcggccatga gaggaatgct ccggtgcggg attggttgca attgcaacta
      721 taatcgcggc gctgcggagt caagcgccgc ggtgcttagc cggcccggcg cagggcttcg
      781 taatgggcga acagctcggt gaaatggccg cgcatggtct gcaccgcgct ggcggtgcgc
      841 ggccagcctg tcgccgccat ggtcaggatc gcttcggcca gcgcctcggg atcggcggcg
      901 gtataggcaa tgccggcgcc gtccgccgcg tgatcggcag cgccgccgcg gtccggcacg
      961 atcaccggca cgccgctggc ccgcgcctcg gcggcggaga ggcagaaggt ctcggcctcg
     1021 cagccgtgga ccagcgcgtc ggcgctggcc aggatcgcgg caaagcgagc gcggttgcgc
     1081 tcgggccgca gcaggcggat gtggggattg gcgccgatgc ttttcaggat cttgcgctcc
     1141 tgcccgccgc cgcccagcag gaccagccca accggcgcgt gggtgctggc ggccatcacc
     1201 gcgtcgatca ccatcggcca gcgcttttcg gcggagagcc ggccaacccc gatcagcagc
     1261 ccggcctctt ccggcaggcc gcacagctcc agcagctggc ggcgcagcgc cggatcgcgg
     1321 cggtcgggcg cgaagacgcc ttcttccacc cccatcggga tcagcgcggt gttggcgacg
     1381 ccgccttcct ccagccgctc gcgcagttcg ccgctggcgc agaccacggt gtcgaactcg
     1441 gtccccagcc gccgcaggtg ctgccagaag ggttcgaacc ggcggtcgat cgtctcgcgg
     1501 ctgaagatcg gctccagcac gcggtaagca taggccgaga gcggatcggc gtgcatgaac
     1561 agcgagcgcg gcgcggaccc tttccagcgc gccaccatcg acgggctgcg ccagggcgag
     1621 ctggcctcga cgaagtcggg gcgcagcgaa tccagcgcgt cgtgcagcgc gtcctcgtcg
     1681 tcgaagtacc agtagttgcg gtccagcgga aagcgggggc tggggatcgt cacgatccgc
     1741 gcctgcggcc cccggtcgat cacttcatag cggtcgcccg gggccaggat cacgatctcg
     1801 tggcccagtt ccggccccag ccgcagcttc tgctcgacat aggtcttcac cccgccgccg
     1861 tggggggtgt agaacgcgca gacatcgaca atgcgcatgg cgcgcggccg gtcctacttc
     1921 ttcttgatcg gagagacgcc caggccgttc ttgtagttca tggtcaccgc gatccgcgaa
     1981 accccgccgc tggccacgac cacgcgggcc ttggcggcct tgggcttgga ccagcccagc
     2041 ttggggttgc cggaaaagcc gatcccgtcg atcttccagc cgaacttgcg gttgctgtca
     2101 cggtcatgca gcaccgagac cgaataggtc cccggccccg ggatccgcac gcagaggaca
     2161 accgggcccg aagccggcgt cggcacctcg acccggcgga agaccttgcc ggcggcgacc
     2221 agcacgttgt cgtcggccag gaaatcggtg tcgttggagg gatagacctc cagcttcatg
     2281 ttgccgaccc ggtccttcat cccgacggct tcgaccagca gtgccggacc cggctcgccc
     2341 gggcggcagc gcccttcggc cttgcccaga tcggggctgg agcgcagcgc cggtccggct
     2401 gccagcagca gcggcacggc cacggtcagg agcatgggca ggaaaacgcg cttcatttca
     2461 ctttctccgc atcgaacagg tccagcaggg caaacagccc gccgaccacc aggtgggtta
     2521 tcagcagcag aactgccatt aaggtcatca ggccggcaat ttcaacatcc tggcccagca
     2581 ggaacaccgc gatcccggca aagaccacgt ccttgttggg cagcagcggc aggcgcgaaa
     2641 ccagcatgcg cagcgttgcc aggacaatcc acagcgccac ctccacttgc ggcagaacca
     2701 ggtgccacat caccgcagac aggatcacca ccgcggcaat ccgcagcaga tgaacccccg
     2761 aaatgaacca caattcgcgt tgcgccaggc tgaacagctt ctgccggaac agcaggatcg
     2821 cgaacgaggt gaccagcacc acgcccagcg agatgaacac cgtgcggcct tccagcccca
     2881 gcatgcccga ggcgatcagc ggccaggccc aggccagcat ggccagcgtg gcgaagttgc
     2941 cgaccagtgc ggacaggatc gtcacgtcct tgatcgcgcc gaacggggcg gccacgaaat
     3001 tgagccgcgc gcgggcccag gcatagaact gcgcctcgcc cagatagccc agcaggatct
     3061 cgttggagac aagcttgcgg atcagcgcgc ccagcccggc gatcggcatg gcccagagcc
     3121 gccggtagat gaaccattcg ctgaccggcc cggccaggta
//
LOCUS       DASUDV010000533         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1034662,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000533 DASUDV010000000
VERSION     DASUDV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034662"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1220
                     /gene="hemW"
                     /locus_tag="VFV30_11675"
     CDS             69..1220
                     /gene="hemW"
                     /locus_tag="VFV30_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX4848796.1"
                     /translation="MARALYIHWPFCLAKCPYCDFNSHVRESVDHDAWQAALLAEMRQ
                     EHAHAGGERLESIFFGGGTPSLMPPALVEALLREAEALWGFAEGIEITLEGNPSSVEA
                     ANYAALAGAGINRVSLGLQSLEDGALRFLGRLHNADEGLRALDVAQRHFARVSFDLIY
                     ALPGQTPEQWQAELERALGFGTGHLSLYQLTIEPGTRFETMVRRHEFAPLDDDAAADL
                     FDLTRVLTGAAGLPAYEISNHARPGEESRHNLAYWRYQDYAGIGPGAHGRRGGVATVR
                     HKKPENWLAAVAAKGDGLAEERALSPAEQASEALMMGLRLAEGVNLAGLRARFGYPVS
                     AMVSGEKVDFYQLKGMVGQDGDRLWITDAGMPLLDGLLGELICAELVAE"
     gene            1217..2110
                     /locus_tag="VFV30_11680"
     CDS             1217..2110
                     /locus_tag="VFV30_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX4848797.1"
                     /translation="MTRADILEAWHDQLSQGRRRSPHTVRAYVATAARLLDAVEVDSW
                     GALARLDAAALRQQLARRRAEGLTNASAARELSALKGFIAFAREQAGLADPAPPRMRG
                     PRIKKGIPRPVTPDEAVNLAAMVDEDAAEEWIGSRDRAVLLLLYGAGLRIAEALSLTG
                     ASLPLGETLVVTGKGNKQRVVPLLPLVRDAVADYATKCPWPLPKDGPLFRGAKGGRLS
                     QGMVQKAMARARIALGLPASATPHALRHSFATHLLGAGADLRSLQELLGHASLGSTQI
                     YTRVDAATLLDVYRSAHPRGD"
     gene            complement(2107..2712)
                     /locus_tag="VFV30_11685"
     CDS             complement(2107..2712)
                     /locus_tag="VFV30_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX4848798.1"
                     /translation="MNEFILRLIEQGGYWGIFALMVIENVFPPIPSEVIMGLGGVAVA
                     RGTMDFWTLLLVGTAGATLGNYVWYMVGDKLGYRRLQPFVDRWGRWLTLEWDDVEKAT
                     RFFRRHGAWIVFALRFSPLLRTIISLPAGLTHMRRWLFLLFTFAGTLVWNAALILGGQ
                     WLASLIESSSLLMDLIVFGLIGAALVGYIYRVITWKPRNPS"
     gene            complement(2722..3681)
                     /gene="gshB"
                     /locus_tag="VFV30_11690"
     CDS             complement(2722..3681)
                     /gene="gshB"
                     /locus_tag="VFV30_11690"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX4848799.1"
                     /translation="MTLRVAVQMDPLESINIAGDSSFALIEAAQARGHTVYEYGVQTL
                     AYESGRLTAWAAPVTVQRVVGSHFTRDEHRRIDLAKDVDVVLMRQDPPFDLSYITATH
                     LLERLEGQTLVVNDPASVRNAPEKVYVLDFAHFMPPTLIARRIEDVRDFHARHPGDLV
                     MKPLHGNGGKAVVRIPADGSNLGALIELFDNAWVEPHMFQPFLPEISEGDKRIVLIDG
                     EIAGAINRRPGEGEFRSNLAAGGYAEPTTLNAREEEICAALGPALKERGLIFVGIDVI
                     GGKWLTEINVTSPTGIMAIDRFNGTNTPARIWEAIEARLHTHG"
BASE COUNT          599 a         1275 c         1305 g          593 t
ORIGIN      
        1 gcaatcaggc gggcaagctt catgggtctt ccttgacatt gcggcgttaa gctagcctga
       61 agaccagcat ggcccgcgcg ctctacatcc attggccgtt ctgcctcgca aagtgtccct
      121 attgcgactt caacagccat gtccgcgaat ccgtcgacca tgacgcctgg caggccgcgc
      181 tccttgccga aatgcgacag gagcatgccc atgccggcgg cgaacggctg gaatcgatct
      241 tttttggcgg cggcaccccc agcctgatgc cgcccgcgct ggtcgaggcg ctgctgcgcg
      301 aggccgaggc gctgtggggc tttgccgagg ggatcgagat cacgctggag ggcaatccct
      361 cctcggtcga ggcggcgaac tatgccgcgc tggcgggcgc cgggatcaac cgggtgtcgc
      421 tgggcctgca gtcgctggag gatggcgcgt tgcggttcct gggccggctg cacaacgccg
      481 acgaggggct gcgcgcgctg gacgtggcgc agcgccattt cgcccgggtc agcttcgatc
      541 tgatctatgc cctgccgggc cagacgcccg aacaatggca agccgaactg gagcgcgcgc
      601 tgggctttgg caccgggcac ctctcgctct accagctcac gatcgagccg ggcacgcggt
      661 ttgagacgat ggtccgccgt cacgaattcg ccccgctgga cgacgatgcc gcggccgacc
      721 tgttcgatct gacccgcgtc ctgaccggcg ccgccggcct gcccgcctat gagatcagca
      781 accacgcccg ccccggcgag gaaagccgcc acaacctggc ctattggcgg taccaggact
      841 atgccgggat cggcccgggc gcacacggcc ggcgcggcgg ggtggcgaca gtgcggcaca
      901 agaagcccga gaactggctg gcggcggtgg cagcaaaagg cgacggcctg gccgaggagc
      961 gcgccctctc ccccgccgag caggccagcg aagcgctgat gatgggcttg cggctggcgg
     1021 agggcgtcaa ccttgccggc ctgcgcgcgc ggttcggcta tcccgtctcc gcaatggtga
     1081 gcggcgagaa ggtcgatttc taccagctca agggcatggt cggccaagac ggcgaccggc
     1141 tgtggatcac cgacgcgggc atgccgctgc ttgacggcct gctgggcgaa ctgatctgcg
     1201 cggagctggt ggcggaatga ctcgcgccga tatcctggag gcctggcacg accagctgag
     1261 ccagggccgg cggcgttccc cccatacggt ccgcgcctat gtcgccactg ccgcgcggct
     1321 gctcgatgcg gtggaggtgg acagctgggg cgccctcgcc cggctcgatg cggccgccct
     1381 gcgccagcag ctggcccgcc gccgggccga aggcctgacc aacgccagcg ccgcgcgcga
     1441 gctgtccgcg ctgaagggct tcatcgcctt tgcccgcgag caggcgggtc tggccgatcc
     1501 ggctccgccc cggatgcggg ggccgcggat caagaagggc attccccgcc cggtcacgcc
     1561 ggacgaggct gtcaatttgg ccgccatggt ggatgaggac gcggccgagg aatggatcgg
     1621 ttcgcgcgac cgggcggtgc tgctcctgct ctatggcgcg gggctgcgga ttgccgaagc
     1681 gctgtcgctg accggggcca gcctgccgct gggcgagacg ctggtggtga ccggcaaggg
     1741 caacaagcag cgggtggttc cgctgctgcc gctggtgcgc gatgccgtgg ccgactatgc
     1801 caccaagtgc ccctggccgc tgcccaagga cggtccgctg tttcgcggcg cgaagggcgg
     1861 acggctcagc cagggcatgg tgcagaaggc catggcccgc gcccggatcg cactgggcct
     1921 gccggccagc gccacgcccc acgcgctgcg ccactccttc gcgacccatc tgctgggcgc
     1981 gggggcggac ttgcgctcgt tgcaggaact gctgggccac gccagcctgg gatcgaccca
     2041 gatctacacg cgggtcgatg cggccaccct gctcgatgtc taccgcagcg cccatccgcg
     2101 cggggactag gacgggttgc gcggcttcca ggtgatgacg cggtagatgt acccgacaag
     2161 ggccgcgccg atcaggccga agacgatcag gtccatcagc aggctggagc tttcgatcag
     2221 gctggccagc cactgtccgc ccaggatcag cgccgcgttc cacaccaggg tgccggcgaa
     2281 ggtgaacagc aggaacagcc agcggcgcat gtgggtcaaa cccgcgggca gcgagatgat
     2341 cgtgcgcagc agcggcgaaa agcgcagcgc gaagacgatc caggcgccgt gccggcggaa
     2401 gaaccgggtg gccttctcga catcgtccca ttccagcgtc agccagcgcc cccaccggtc
     2461 gacgaagggt tgcagccggc gatagcccag cttgtcgcca accatgtacc agacatagtt
     2521 gcccagcgtt gccccggccg tgccgaccag cagcaaggtc cagaaatcca tcgtcccgcg
     2581 ggccacggcg accccgccca gccccatgat cacttcgctg ggaatcggcg gaaacacgtt
     2641 ctcgatcacc atcagggcga agatgcccca atagccgccc tgctcgatca ggcggaggat
     2701 gaactcgttc atggggtttg ctcagccgtg ggtatgaagc cgcgcttcga tcgcttccca
     2761 gatgcgggcc ggggtgttgg tgccgttgaa ccggtcgatc gccatgatcc cggtcggcga
     2821 ggtcacgttg atttcggtca gccacttgcc gccgatcacg tcgatgccga cgaagatcag
     2881 gccgcgttcc ttcagcgccg ggccaagggc cgcgcagatc tcttcctcgc gggcgttcag
     2941 cgtggtcggt tcggcatagc cgcccgcggc caggttcgaa cggaactccc cttcccccgg
     3001 ccggcggttg atcgccccgg caatctcgcc gtcgatcagc acgatccgct tgtcgccctc
     3061 gctgatctcg ggaaggaagg gctggaacat gtgcggctcc acccaggcgt tgtcgaacag
     3121 ttcgatcagc gcgcccaggt tcgatccgtc tgccgggatc cgcaccacgg ccttgccgcc
     3181 gttgccgtgc agcggcttca tcaccagatc gccagggtgg cgggcgtgga aatcgcggac
     3241 gtcctcgatc cggcgggcaa tcagcgtcgg cggcatgaag tgcgcgaaat cgagcacata
     3301 gaccttttcc ggcgcgttgc ggaccgaggc cggatcgttg accaccaggg tctgcccctc
     3361 gagccgctcc agcaggtggg tggcggtgat gtaggacagg tcgaacggtg ggtcctggcg
     3421 catcagcacc acgtcgacgt ccttcgccag gtcgatccgg cggtgctcgt cgcgggtgaa
     3481 gtggctgccc actacgcgct gcaccgtcac cggcgcggcc caggcggtca gccgcccgct
     3541 ttcataggcc agggtctgga ccccgtattc gtaaaccgtg tgcccgcgcg cctgcgccgc
     3601 ctcgatcagg gcaaagctgg aatcgccggc gatgtttatg ctttcgagcg ggtccatctg
     3661 gacagcgacg cgaagggtca tgcggggtcg gctccaatca cagcaacagg gcgttcgcca
     3721 tgtggcgtgg gaagcgcccc ggcgcaagga gcagcacgtc gatccgctgc ac
//
LOCUS       DASUDV010000534         4611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_747488,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000534 DASUDV010000000
VERSION     DASUDV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747488"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFV30_11695"
     CDS             complement(<1..472)
                     /locus_tag="VFV30_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4848800.1"
                     /translation="MSKAELVSYEPATGAELWRGKHGNVDDIVGRARRAWPAWAAQPL
                     ANRMELVRRFANEVRKESEKLATLIARETGKPLWEARTEVESVINKVEISIRAYAERT
                     AQRKLDNALQGTAAVRHKPHGVMAVLGPYNFPAHLPNGHIIPALIAGNAIVFKPS"
     gene            663..1553
                     /locus_tag="VFV30_11700"
     CDS             663..1553
                     /locus_tag="VFV30_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4848801.1"
                     /translation="MNAVPSPTAPAGQAYADRYWTSSDGLRLHFRDYPGRADRPPVLC
                     LPGLTRNGRDFSHVAERLSGEWRVICPDLRGRGDSAYAKDSATYIPLQYAEDLTLLLE
                     QEGIGRFVSVGTSLGGLITMIFAMTMPDRLAGALLNDVGPEIAPEGLERIRGYVGQGR
                     SFPTWMHAARALQEMQGEVFPDYAIADWLAMAKRGMVVGGNGRIVFDYDMKIAEPLSQ
                     PGGEAGIDLWPAFGALTGRPVALLRGELSDILAEATLERMAGMLSDAEAVTVPRVGHA
                     PMLDEPAAMAAIDRLLARVR"
     gene            1570..2697
                     /locus_tag="VFV30_11705"
     CDS             1570..2697
                     /locus_tag="VFV30_11705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4848802.1"
                     /translation="MRLLHLHSSFNPGGKELRAAKLIGAFGAQVRHTIVSGVPGAMGA
                     AGAIDPAMKVDYPQDFPPLSGPVSLARLRAVAEAMRGHDLVLTYNWGAMDAVMAHRLY
                     ARRLGLPPLVHHEDGFNQDEAEGLKPARNLFRRLALPTAAALVVPSQTLERIALDIWK
                     QPRGRVRRVPNGIALADFARPPRLDAVPGLVKQPGDLWIGTLAGLRAVKNLPALVEAF
                     RAVPEPWKLVIVGEGPERAAIAGRAAELGLSDRVFLPGHAPDPAAVMPLFDLFALSSD
                     SEQFPISVVEAMAAGIAVASTDVGDVAAMLSDANRPLVDGVRDAGALGQVLERLAADP
                     ALRAAIGQANKARAEAEYGEAAMVAAYRAIYGGALGRQSFP"
     gene            2819..3607
                     /locus_tag="VFV30_11710"
     CDS             2819..3607
                     /locus_tag="VFV30_11710"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4848803.1"
                     /translation="MALRPPSEQEPPKPGSRDAVAQDGFLREVDEALREEQIVNSFKR
                     YGKPVGLAIAVGLLGLAGYLWWDNSTKAEGATRSERTILALDRLAAGELDAASKDFQA
                     LAKEPNDGTRAVALMQLAAIAVQQGKLEDAAKQFAAIAADPKLPQTYRDLATVREVAL
                     RFDSMKPEEVIARLKPLAVPGNAFFGSAGELVGMAYLEQGKPELAGALFAQIGKDPKV
                     PQSLRVRMRQMAGGLGFDAGDELPPEQPEGQGAAPPPAPAPAKQ"
     gene            3638..>4611
                     /locus_tag="VFV30_11715"
     CDS             3638..>4611
                     /locus_tag="VFV30_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4848804.1"
                     /translation="MNSNTITRRLSAPLALALALGLAGCGAFGGKDKPATPTVGNRVP
                     ILSKIDTSAKVDPALANVAVVMPPATQNDEWPQAGGTPSKAYGNLALSATPAYAWSAR
                     IAGSTDKQRLAAAPVIGGGMLFAMDTEGVLHAFDAKTGAPRWTQGFQIKGDGSTSVYG
                     GGAAYSADRVYVTTGVGEVAALNAADGKVLWKVKPAGPLRGSPTIAFNAVYIMTQSNQ
                     LIALSAENGVQQWSRAASVGQTGVFGVAAPSVGQGTVIAGFSTGELIAFRYENGREVW
                     SDALARTSLSTSVGVLTDIDADPIIDRGRVFALGQGGRMAAYELVAGQR"
BASE COUNT          684 a         1560 c         1636 g          731 t
ORIGIN      
        1 ccgaaggttt gaagacgatc gcgttgccgg cgatcagcgc cgggatgatg tgcccgttgg
       61 gcaggtgcgc ggggaagttg taggggccca gcaccgccat cacgccgtgc ggcttgtggc
      121 ggacggcggc cgtgccctgc aacgcgttgt ccagcttgcg ctgggcggtc cgctcggcat
      181 aggcgcggat cgaaatctcg accttgttga tgacgctctc aacctcggtc cgggcttccc
      241 acagcggctt gccggtctcg cgggcgatca gcgtggcgag cttttcgctc tccttgcgga
      301 cttcgttggc gaagcggcgg accagctcca tccggttggc aagcggctgc gctgcccagg
      361 ctggccatgc ccgccgggcc cgacccacga tgtcgtcaac attgccgtgc ttgccgcgcc
      421 acagttcagc gccggttgcc ggctcatacg aaacgagttc cgccttgctc aatggtctat
      481 ccgtcccttc cccgtgagtt gcgacccagg gatgtgcgtg cgccttagcc gcgttgcaga
      541 tatatggaaa gccttgcgtt tcagataagg acaacggcgg tcgtttcctt gatccggcgc
      601 aaggcgcgcg gcgccttggc gcttgcaccg cgcggcgcgg cgggcgataa cgcggcccga
      661 tcatgaacgc tgtcccttca cccaccgccc ctgccggcca ggcctatgcc gaccgctact
      721 ggaccagcag cgacgggctg cgcctgcatt tccgggatta cccgggccgg gctgaccgac
      781 cgccggtgct gtgcctgccg gggctgaccc gcaacgggcg cgatttcagc cacgtggccg
      841 agcggctgag cggcgaatgg cgggtgattt gcccggatct gcgcgggcgg ggtgacagcg
      901 cctatgccaa ggattcggcc acctatatcc cgctgcaata cgccgaagac ctgaccctgc
      961 tgctggagca agaagggatc gggcggttcg tgtcggtcgg cacctcgctg ggcgggctga
     1021 tcacgatgat tttcgccatg accatgcccg atcggctggc cggggcgctg ctcaacgatg
     1081 tcggcccgga aatcgcgccc gaggggctag agcggatccg cggctatgtt ggccagggcc
     1141 gcagcttccc gacctggatg cacgccgcgc gcgcgctgca ggaaatgcag ggcgaggtgt
     1201 tccccgacta tgcgattgcc gattggctgg ccatggccaa gcgcggcatg gtggtgggcg
     1261 gcaacgggcg gatcgtgttc gattacgaca tgaagatcgc cgagccgctg tcccagccgg
     1321 gcggagaggc cgggatcgac ctgtggcccg cctttggcgc cctgaccggc cgcccggtag
     1381 ccctgctgcg cggcgaactg tccgacattc tggccgaagc gacgctggag cggatggcgg
     1441 gcatgctgtc cgatgccgaa gccgtcaccg tgccgcgggt cggccatgcg ccgatgctgg
     1501 acgaacccgc cgccatggcc gcgatcgacc ggctgctggc ccgcgtgcgc taggcggaac
     1561 ggaaccggtt tgcggcttct gcaccttcat tccagtttca atcccggcgg gaaggagctg
     1621 cgcgcggcca agctgattgg cgcgttcggg gcgcaggtcc gccacaccat cgtttcgggc
     1681 gtgcccggcg cgatgggcgc ggccggcgca atcgatccgg ccatgaaggt cgactatccg
     1741 caggatttcc ccccgctttc gggccccgtc tcgctggcgc ggctgcgcgc cgtggccgag
     1801 gcgatgcgcg ggcacgatct ggtgctgacc tacaactggg gggcgatgga cgcggtgatg
     1861 gcgcaccggc tctatgcccg ccggcttggc ctgccgccgc tggtccacca cgaggacggc
     1921 ttcaaccagg acgaggccga agggctgaag ccggcacgca acctgttccg ccggctggcc
     1981 ctgccgaccg ccgcggcgct ggtggtcccc tcgcaaacgc tggagcggat cgcgctggac
     2041 atctggaagc agccgcgcgg ccgggtgcgg cgcgtgccca acgggatcgc gctggccgat
     2101 ttcgcccgtc cgccgcgtct cgatgcggtg ccggggctgg tcaagcagcc gggcgacctg
     2161 tggatcggca ccctggccgg gctgcgcgcg gttaagaacc tgccggcgct ggtcgaggct
     2221 ttccgggccg tgccggagcc gtggaagctg gtgatcgtgg gagaggggcc cgagcgcgcg
     2281 gcgattgccg gccgcgcggc cgaactgggc ctgtccgacc gggtgttcct gcccggccat
     2341 gcccccgatc ccgccgcggt catgccgctg ttcgatcttt tcgcgctgtc ttcggacagc
     2401 gagcagttcc cgatctcggt ggtcgaggcg atggccgccg gaatcgccgt ggcctcgacc
     2461 gacgtgggcg atgtcgcggc gatgctgtcc gatgccaacc gcccgctggt ggacggggtg
     2521 cgggacgcgg gcgcgctggg ccaggtgctg gaacggctgg cggccgatcc ggcgctgcgc
     2581 gcggcgatcg gccaggccaa caaggcccgg gcagaggccg aatacggcga agcggcgatg
     2641 gttgcggcct accgcgcgat ctatggcggg gcgcttgggc ggcaaagctt tccgtgaccg
     2701 gggccgtgct tgcggccgtt cagggcagct tcacggggga attcgcatgg gaagggcgtt
     2761 gaaaaggccg gtccatccgc taaacagccg ccgacacaca ctttgaaggc attcgatctt
     2821 ggccctgcgt cctccttccg aacaagaacc cccgaagccc ggcagccgcg atgcggtggc
     2881 ccaggacggc ttcctgcgcg aagtcgatga agcgctgcgc gaagagcaga tcgtcaactc
     2941 cttcaagcgc tatggcaagc cggtgggcct ggccatcgcc gtgggcctgc tgggcctggc
     3001 cggctatctg tggtgggaca attcgaccaa ggccgaaggc gccacccgct ccgaacggac
     3061 gatcctggcg ctggaccggc tggctgccgg tgagctggat gccgcgtcca aggactttca
     3121 ggccctggcc aaggaaccca acgacggcac ccgcgccgtg gcgctgatgc agctggcggc
     3181 catcgccgtg cagcagggca agctggaaga cgcggccaag cagtttgccg cgatcgccgc
     3241 cgatcccaag ctgccgcaga cctaccgcga tctcgccacc gtgcgcgaag tggcgctgcg
     3301 gttcgattcg atgaagccgg aagaggtgat tgcccggctc aagccgctgg ccgttccggg
     3361 caacgccttt ttcggcagcg ctggcgaact ggtcggcatg gcctatctgg aacagggcaa
     3421 gccggaactg gccggggctc tcttcgccca gatcggcaag gaccccaagg tcccgcaaag
     3481 cctgcgcgtc cggatgcgcc agatggccgg cggccttggc ttcgatgccg gggacgagct
     3541 gcctcccgaa caacccgagg gccaaggcgc tgcgccgccg cccgctcccg cccccgcgaa
     3601 gcaataagcg ccggcaagcc gatctaagga accgccgatg aattcgaaca ccattacccg
     3661 ccgcctctcc gctccgctcg cgctggccct ggcgctgggc ctggccgggt gcggcgcttt
     3721 cggcggcaag gacaagcccg cgacgccgac tgtcggcaac cgcgtgccga tcctcagcaa
     3781 gatcgatacc agcgccaagg ttgatccggc gctggccaat gtcgccgtgg tcatgccgcc
     3841 ggcgacccag aacgacgaat ggccgcaggc cgggggcacc cccagcaagg cctatggcaa
     3901 cctcgccctg tccgccacgc cggcctatgc ctggagcgcg cggatcgccg ggtcgaccga
     3961 caagcagcgg ctcgccgccg cgccggtgat cggcgggggc atgctgttcg cgatggacac
     4021 cgaaggcgtg ctgcacgcgt tcgacgccaa gaccggcgcc ccgcgctgga cccaggggtt
     4081 ccagatcaag ggtgacggtt cgacctcggt ctatggcggc ggcgcggcct attcggccga
     4141 ccgcgtctat gtgacgaccg gcgtgggcga agtcgccgcg ctgaacgccg ccgacggcaa
     4201 ggtgctgtgg aaggtcaagc cggccggccc gctgcgcggt tcgccgacga tcgccttcaa
     4261 cgcggtctac atcatgaccc agtcgaacca gctgatcgcg ctgagcgccg aaaacggggt
     4321 ccagcaatgg agccgcgcgg cctcggttgg ccagaccggc gtgttcggcg tggccgcgcc
     4381 ctcggtcggc cagggcacgg tgatcgccgg cttctcgacc ggcgaactga tcgccttccg
     4441 ctatgaaaac ggccgcgaag tgtggtcgga cgcgctggcg cggacttcgc tgtcgacctc
     4501 ggtcggcgtg ctgaccgata tcgacgccga cccgatcatc gaccgtggcc gcgttttcgc
     4561 gctgggccag ggcgggcgca tggccgccta tgaactggtc gccggccagc g
//
LOCUS       DASUDV010000535         5603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_659389,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000535 DASUDV010000000
VERSION     DASUDV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659389"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1377)
                     /locus_tag="VFV30_11720"
     CDS             complement(175..1377)
                     /locus_tag="VFV30_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848805.1"
                     /translation="MTLNFSAGWNELSDFGSLTDWSAGVTWGVTEKLNLQASYISNQA
                     APSLGQLGNPQAVTFNVPVYDFTRGENVLVTVTSGGNPALLREQQRDIKLSANWTLPI
                     PSSNLIVEWFRNRSTNVTSSFPLLTPAIEAAFPGRVTRDGSGRLISIDQRPITFAAQN
                     TSRLRWGFNTSGGIGKQPDANGMMAPAAPRPGGAPGARPAGAGPTPPAAGAPRPAGAG
                     PRGPGGRGGSGFMGAMFGGGGPPGRWSLGVYHAVQFENRVTVSPTGPVLDQLNGEALS
                     TNGSPRHSIEFNGGVFYKGVGGFFQGTWISPSTLKASGLPGTSDLRFGSNTNVTLFLF
                     AEFSTMPGVTKAVPFLKGAQLSVRVENLFDSVQTVTDGTGTIPLSYQRSYLDPRGRVI
                     TVGFRKAF"
     gene            complement(1669..2766)
                     /locus_tag="VFV30_11725"
     CDS             complement(1669..2766)
                     /locus_tag="VFV30_11725"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX4848806.1"
                     /translation="MRTFVTLASLLAASPVLAQTSEPAPQPAPQPASDAPAAEAEQTP
                     EEKLEETAAPGEILVVASRLRGQIEAPQAPIASFDEDEIQALGATSVADLLTKVSPQT
                     SSGRGRGGAGGMPLVLVNGQRVTNFRELRNYPPEAIKRVEILPEEVALRYGSPPNTRV
                     VNLILKDNFSSRRIEGSYGIPTRGGFNTWQGEATLLKIQKLNRFSVTASTSDTSPLFE
                     SERDLVQTAGNRPTVSTDPDPAANRTLISDSRNFGLNAAWTKGFGKDGTKGSFTVSAE
                     ANRSDSRGFSGLNTVLLTAPGGATALRSLPGPLERVNQTTSASAGLGYNKMLGRWQFS
                     ATVDGTHTESKSRFDRRADVSGLVAQAAAGT"
     gene            3001..3249
                     /gene="infA"
                     /locus_tag="VFV30_11730"
     CDS             3001..3249
                     /gene="infA"
                     /locus_tag="VFV30_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX4848807.1"
                     /translation="MAKEELLEMRGQVVELLPNAMFRVRLENDHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFMPGRGGPGQFG"
     gene            3250..3828
                     /locus_tag="VFV30_11735"
     CDS             3250..3828
                     /locus_tag="VFV30_11735"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX4848808.1"
                     /translation="MSTPALVLASSSPRRRDLLARIGAAPARIESPDIDESPLTGELP
                     RAYALRLAQAKAAAVNRHEGEIVLAGDTTVAVGRRILPPAETEAIQRELLGLISGRRH
                     RCISAVCLIDTAGKARTRVVETVVTFKRLTPDEIDAYVASGEGLGKAGGYAIQGRAEA
                     LIRYVAGSHSAVIGLPLFETRALLKAAGYPLG"
     gene            3821..4780
                     /locus_tag="VFV30_11740"
     CDS             3821..4780
                     /locus_tag="VFV30_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX4848809.1"
                     /translation="MAEWLIEDGIGEERAVLVDGGEAAAARVRWPGGLEAGLVADAVL
                     VSRAAGSRRGTARFPSGEEALVDSLPREASEGAALRLKVTRAAMAERGRFKRAQARPT
                     DEAPAPAPTLAKALAGKTVPQFPAGLWEDVMADAASGEAAFPGGALTITPTPALTVID
                     IDGDLPPRALALAAVPALAAAVRRLDLGGSIGIDFPSLSDKADRKAVDEALAAALSGW
                     PHERTAMNGFGLVHLVARLERPSLLHRLALDPVGAAARMLLRRAERVRDPGVLLLTAA
                     PAVRAAVLPAWEAELARRTGRTIRWHEDPALAPGAAFAQALTA"
     gene            4777..4944
                     /gene="yacG"
                     /locus_tag="VFV30_11745"
     CDS             4777..4944
                     /gene="yacG"
                     /locus_tag="VFV30_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX4848810.1"
                     /translation="MTNPVRKCPICGKPRSDAHTPFCSTRCKDRDLVRWLVDGYAIPG
                     PPALDEEEGKA"
     gene            5042..5117
                     /locus_tag="VFV30_11750"
     tRNA            5042..5117
                     /locus_tag="VFV30_11750"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5075..5077,aa:Phe,seq:gaa)
     gene            5281..>5603
                     /locus_tag="VFV30_11755"
     CDS             5281..>5603
                     /locus_tag="VFV30_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC5 family protein"
                     /protein_id="HEX4848811.1"
                     /translation="MLVQVLVALSITALIGYFTWRKVKSQPASHDGSGKDVFLAGGGL
                     SWLYVAGSITLTNLSTEQLVGMNGNQMLLLAWWELAGFVGLLILAFVFVPIYYKHQCT
                     TVTELL"
BASE COUNT          851 a         1888 c         1959 g          905 t
ORIGIN      
        1 actcgctctg gcagtcgacc gtcaacaagg tgatgccgcg cttcctgctg ttcgccctgt
       61 ccagcctgtg cctgatggcg ctgacgctgc acctgatcct ggcggtgttc cactaagccg
      121 gatccgtgaa gagcccccgg ccggcagacc ggccggggcg ctttcagacc gcctttagaa
      181 agccttgcgg aaaccgaccg tgatgacccg cccgcgcgga tcaagatagc tgcgctgata
      241 cgacagcggg atcgtgccgg ttccgtcggt cacggtctgg accgaatcga acaggttctc
      301 gacccgcaca gagagctgcg cgcccttcag gaacggcacc gccttggtca cgcccggcat
      361 cgtcgagaat tcggcgaaca ggaacagggt gacgttggtg ttgctgccaa agcgcagatc
      421 gctcgtcccc ggcaagccgc tggccttgag cgtgctgggc gaaatccagg tgccctggaa
      481 gaagccgccg acgcccttgt agaacacccc gccattgaac tcgatcgagt ggcgcggcga
      541 cccgttggtg gacagcgcct cgccgttgag ctgatcgagc accggcccgg tcggcgaaac
      601 cgtgacccgg ttctcgaact ggaccgcgtg atagacgccc aggctccagc gccccggagg
      661 gccgccgcca ccgaacatcg cgcccatgaa cccgctgccg ccgcggccgc caggaccgcg
      721 cggtccggca ccggcgggac gcggcgcacc ggccgccggc ggggtcgggc ccgcaccggc
      781 cggacgcgcg ccaggcgcgc cgccgggacg cggtgcggcc ggggccatca tgccgttggc
      841 atcgggctgc ttgccgatcc cgcccgaggt gttgaagccc caacgcagcc gcgaggtgtt
      901 ctgggcggca aaggtgatcg gccgctggtc gatcgaaatc aggcggcccg aaccgtcgcg
      961 cgtcacccgg cccgggaagg ccgcctcgat cgccggggtc agcagcggga agctggaagt
     1021 gacgttggtc gaccggttcc ggaaccattc gacgatcagg ttcgaactgg ggatcggcag
     1081 ggtccagttg gcggacagct tgatgtcgcg ctgctgttcg cgcagcagcg cggggttgcc
     1141 gccgctggtg accgtcacca gcacgttttc cccgcgggtg aaatcataga ccggcacgtt
     1201 gaacgtgacg gcctggggat tgccgagctg acccagcgag ggtgccgcct ggttggagat
     1261 gtagctggcc tgcaggttga gcttttcggt cacgccccag gtcaccccgg cgctccagtc
     1321 ggtcagcgag ccgaaatcgg acagctcgtt ccagccggcg ctgaagttga gcgtcacgtc
     1381 gccgatgccc gcgccaaacc gctcgcgccg gctggtgatc ggaatgccga cattgatccc
     1441 caggctgaga tcgccgcggc tcaggctgac cgggccgagc aggctgcggg tgtcggtgct
     1501 gtcgatcccg gaccagccgt agcccgcctt gaacgtggtg ctgagatagc cgccgggcag
     1561 gcggatcggc cggccgctga gcgtgacgag cgagctgacg ctgtcgacat tgctggtggt
     1621 cgtatcaaag cccggatcgg tcagcgcggg cagcggcccg gtgatcggct aggtgccggc
     1681 agcggcctgc gccaccaggc cggacacgtc cgcccggcga tcgaaccgcg acttgctttc
     1741 ggtgtgggtg ccgtcaaccg tggcactgaa ctgccagcgg cccagcatct tgttgtaacc
     1801 cagaccggcc gaggccgagg tggtctggtt cacccgctcc agcggtccgg gcaggctgcg
     1861 cagcgcggtc gcgccgccgg gcgcggtcaa gagcacggtg ttgaggcccg aaaagccccg
     1921 gctgtccgac cggttggcct cggcgctgac cgtgaacgag cccttggtcc cgtccttgcc
     1981 gaaccccttg gtccaggcgg cgttgaggcc gaaattgcgc gaatccgaga tcagcgtgcg
     2041 gttcgcggcc ggatcgggat cggtcgaaac ggtcgggcgg ttgccggcgg tctggaccag
     2101 atcgcgctcg ctttcgaaca gcgggctggt gtcgctggtg ctggcggtca cgctgaagcg
     2161 gttgagcttc tggatcttga gcagggtcgc ttcgccctgc caggtgttaa agccgccccg
     2221 ggtcgggatg ccatagctgc cttcgatccg gcggctggag aaattgtcct tgaggatcag
     2281 gttgaccacc cgggtgttcg gcgggctgcc ataccgcagc gcgacttcct cgggcaggat
     2341 ctcgacccgc ttgatcgctt cgggcggata gttgcgcagt tcgcggaagt tggtgacgcg
     2401 ctggccgttg accagcacca gcggcatccc gcccgcgccg ccgcggccgc ggccggagct
     2461 ggtctgcggg ctgaccttgg tcagcagatc cgccaccgag gtggcaccca gcgcctggat
     2521 ctcgtcctcg tcaaagctgg caatcggcgc ctgcggcgcc tcgatctggc cgcgcaggcg
     2581 gcttgccacg accaggattt cgccaggcgc ggcggtctct tccagctttt cctccggcgt
     2641 ctgctcggcc tcggcagccg gggcatcgct agccggttgc ggagctggtt gcggagccgg
     2701 ttcgctggtc tgggcaagaa ccggcgaggc ggccagcagg ctggccaggg ttacgaaagt
     2761 gcgcatggtt gttccggaaa tccttatgga aaccaatccc tgggcggttg cagcaccctt
     2821 gttcccccag cgctgcaatg cagcgtagcg gaatccaagg caatgccacc ggtcgtatca
     2881 aattgtcgct tcgtagaacg gggcctgaac gcccatgcca gccaaaggct ttccttttgc
     2941 gccccgcggg gctatggggc gcggcaattc attcaacctt atcatggaaa ccaggcccgg
     3001 atggctaagg aagaactgct cgaaatgcgc ggccaggtgg tcgaactgct gcccaacgcg
     3061 atgttccgcg tgcggctgga aaacgatcac gagatcctgg gccacaccgc gggcaagatg
     3121 cgcaagaacc gcatccgcgt gctggttggc gacgaagtgc tggtcgagct gaccccttac
     3181 gacctgacca agggccggat tacctatcgc ttcatgccgg gccgcggcgg ccccggccag
     3241 ttcggctaat tgtccacccc cgcgctcgtc cttgccagct cgtccccgcg ccggcgcgat
     3301 ctgctcgcgc ggatcggtgc ggcgccggcc cggatcgaaa gccccgacat cgatgaaagc
     3361 ccgctgacgg gcgaactgcc gcgggcctat gccttgcggc tggcccaggc caaggcggcc
     3421 gcggtgaacc gccatgaggg cgagatcgtg ctggccgggg ataccacggt cgcggtcggc
     3481 cggcggatcc tgcccccggc cgaaaccgaa gcgatccagc gcgagctgct gggcctgatc
     3541 tctggacgcc ggcaccgctg catttccgcc gtctgcctga tcgacaccgc cggcaaggcc
     3601 cggacccggg tggtcgaaac cgtggtcacc ttcaagcgcc tgactccgga cgaaatcgac
     3661 gcctatgttg ccagcggcga aggcctgggc aaggccggcg gctatgctat ccagggccgc
     3721 gccgaagcgc tgatccgcta tgtcgcgggc agccattcgg ccgtgatcgg cctgccgctg
     3781 ttcgaaaccc gggcgctgct gaaagcggcg ggataccccc ttggctgagt ggctgatcga
     3841 agacgggatc ggcgaggagc gcgcggtcct ggtggacggc ggggaagcag cggcggcgcg
     3901 ggtgcgctgg ccgggcgggc tggaagccgg gctggtggcc gatgcggtgc tggtttcgcg
     3961 cgcagccgga tcgcggcgcg gcacggcccg ctttccctct ggcgaagagg ccctggttga
     4021 cagccttccg cgcgaggcca gcgaaggcgc ggcgctgcgc ctgaaagtga cccgcgcggc
     4081 catggccgaa cgcggccggt tcaagcgcgc ccaggcccgc ccgacagacg aagcgccggc
     4141 cccggccccc accctcgcca aggcgctggc cggcaagacc gtcccgcaat ttccggctgg
     4201 cctgtgggaa gacgtgatgg ccgatgcggc cagcggcgaa gcggcctttc ccggcggcgc
     4261 gctgaccatc acccccactc cagccctgac cgtgatcgac atcgacggcg acctgccccc
     4321 gcgcgccctg gcgctggcag ccgtgccggc cctggccgcc gcggtccgcc gtctggacct
     4381 gggcggttcg atcgggatcg actttcccag cctgtccgac aaggccgacc gcaaggcggt
     4441 ggacgaggcg ctggccgccg ccctgtctgg ctggccgcac gagcggaccg cgatgaacgg
     4501 gttcgggctg gtgcatctgg tggcccggct ggaacggccg tcgctgctgc accgcctggc
     4561 gctggacccg gtgggtgcgg cggcccggat gctgctgcgc cgggccgagc gggtgcgcga
     4621 tcccggcgtg ctgttgctga ccgccgcgcc cgcggtgcgc gccgcggttc tgccggcgtg
     4681 ggaggccgaa ctggcccggc ggaccgggcg aacaatccgg tggcacgaag acccggctct
     4741 tgcgcccggc gccgctttcg cccaggcgct gacggcatga cgaatccggt ccggaaatgc
     4801 ccgatctgcg gaaagccgcg cagcgatgcg cacaccccgt tctgctcgac ccggtgcaag
     4861 gaccgcgacc tggtgcgctg gctggtcgat ggctatgcca tacccggacc gcccgcgctg
     4921 gacgaagaag aaggaaaggc ctgagggctg ttcaaacccc cttgccaagc gcgcccggct
     4981 tcgccatagg cgcgcttccc atcgcgcggt gcccttttcg ggggcctgtc gccgaagcga
     5041 tgcccggata gctcagttgg tagagcagcg gattgaaaat ccgcgtgtcg gtggttcgaa
     5101 tccgcctccg ggcaccactt tcccctcagc gtggattgcc ttgcccgtcc ggtctgcgcc
     5161 tgcagcctga gcgctgcccc gttccattcg tatgccggtc ccttgcaacc gattgccgta
     5221 aggggcaaca acggcacagg cgcagaccgg gaggccggat cggcgccgga agggggagcg
     5281 atgttggttc aagtcctggt ggcgctgtcg atcacggcgc tgattggcta tttcacctgg
     5341 cgcaaggtca agagccaacc tgccagccat gacgggtccg gcaaggacgt gttcctggcc
     5401 ggcggcgggc tgagctggct ctatgtcgcc gggtcgatca cgctcaccaa cctgtccacc
     5461 gagcagctgg tcgggatgaa cggcaaccag atgctgctgc tggcctggtg ggaactcgcc
     5521 ggcttcgtcg gcctgctgat cctcgccttc gtcttcgtgc cgatctacta caagcaccag
     5581 tgcaccacgg tgaccgagct gct
//
LOCUS       DASUDV010000536         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1940784,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000536 DASUDV010000000
VERSION     DASUDV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940784"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1563
                     /locus_tag="VFV30_11760"
     CDS             43..1563
                     /locus_tag="VFV30_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX4848812.1"
                     /translation="MQTSFALIDWAVLGGYVLLLALAGWLTTRRGMRSEDYFLAGHHA
                     PTWLVAISVLSTVQSAATFLGVPDNSFRRDYTYLGGVLAALIAAWIVANVLIPRFYAL
                     KVTTVYELLESRFDATARRAAAAMYLVGRIFASGARLYLAAIAVSMILFLDVTPQHIA
                     IAAFALLVFGLVFTFMGGLNSVIWSDLVQVTLYVGAALLVAWHLWSGLDLTVAEAVTA
                     LRHTPDGTDKLALINPTLDLSAPWGLFAVLTGVMLLNLASSGLDQDITQRLLACEDAK
                     KGARSLYVSVWMSLPVILLFLVIGSLLYLHYQGSAAGTFRGEKVTVFMHYILTEIPPG
                     LRGLITVGVIAAAAINSGLISMSAVLVNDLYRPFAGVRTEAHFVRMGRIASVVLGLAL
                     FAMAILSYYWQRYADSALLDFVLGVMAFAYSGLIGVYGVALFSRRGSSASVIAAFVAG
                     FLTVLAFQPYVIDSLGLPAALKGVAFPWQLVLGSAVAALVCLSAPGKGVAQAAASR"
     gene            1575..1961
                     /gene="crcB"
                     /locus_tag="VFV30_11765"
     CDS             1575..1961
                     /gene="crcB"
                     /locus_tag="VFV30_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX4848813.1"
                     /translation="MNTGTFLSHSALVALGGGIGAWLRFAAGRLIGAGAFPWATLSVN
                     LIGSLAMGLLAGWLARHGQGGEQWRLLLGVGVLGGFTTFSAFSLELVNLIQRGQGGMA
                     ALYAAVSVIGGIAGLFAGLALARTFA"
     gene            1958..3145
                     /locus_tag="VFV30_11770"
     CDS             1958..3145
                     /locus_tag="VFV30_11770"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX4848814.1"
                     /translation="MTERGIIPADTVRQFTVAADDDGVRLDRWFKRHLPQVGFATVSR
                     WARTGQIRVDGKRADVADRLSAGQVVRVPPGGDSPDKQTRARKPLTEEQIALAEGMVI
                     SKDRAAIVLNKPPGLATQGGSGTFQHVDGLLDAYADDGPRPRLVHRLDKDTSGVLLIA
                     RTPGSAAYFSKRFSGRSARKIYWALVIGVPDIDDGVIELPLAKQPGTGGEKMMVDKSG
                     EGQSARSRYRVIDRAGNRAAWVELQPLTGRTHQLRVHMAAIGHPIVGDGKYGGPDAFL
                     SGSISRKMHLHARRLIIDHPDGDMIDVRADLPEHFAASLEQLGFDEADGDAMELVLAK
                     PAPAKEDKKAAAKAHAKQIRKERRGERRSRAGGASPVRKPPTKRGPTKGKGSPKPGAP
                     KKR"
     gene            3145..3747
                     /locus_tag="VFV30_11775"
     CDS             3145..3747
                     /locus_tag="VFV30_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX4848815.1"
                     /translation="MHPNAAFRHPDRDLLETLIDQIGFGMVFAQTPDGPRVAHVALHS
                     TGDGAVQFHISRGNALAKHLAGSTALVVVNGPDGYVSPRWYSDPAQVPTWNYVALELE
                     GRVRRMDAEATLALVTDLSDKHEARVAEGTPWTMDKLPPEKLRGLLAGIVGFELEVQA
                     WRETVKLSQNKDAAERERLAAGLESEGAVGIAQLMRTLAK"
     gene            3744..>4322
                     /locus_tag="VFV30_11780"
     CDS             3744..>4322
                     /locus_tag="VFV30_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4848816.1"
                     /translation="MTLRLALFDCDGTLVDGQAGVVNAMEAAFAAVGLAPPDRHQVRR
                     VVGLSLPQAIRLLAPAAPDGQRTAADAAYREAFRTARESGALVEPLYDGIAALVAQLD
                     EAGWLLGVATGKSERGLNHCLATHGLSARFCTLQTADHHPSKPHPSMVEAALAETGVE
                     RARCVMIGDTQFDMAMAVNAGVRALGVDWGYHT"
BASE COUNT          611 a         1475 c         1528 g          708 t
ORIGIN      
        1 gcgaccgtga gcacgcgctg agccggttgc agtagggccg caatgcagac atcgttcgct
       61 cttatcgatt gggccgtgct gggcggctat gtcctgctgc tggcgctggc cggatggctg
      121 accacccggc gcgggatgcg ctcggaagac tatttcctgg ccggccacca cgcgccgacc
      181 tggctggtgg ccatatcggt gctctccacg gtccagtcgg ccgcgacgtt cctcggggtg
      241 ccggacaatt cgttccggcg cgattacacc tatctgggcg gggtgctggc cgcactgatc
      301 gccgcgtgga ttgtcgccaa cgtgctgatc ccgcggttct atgccctgaa agtgaccacg
      361 gtttacgagc tgctggagag ccggttcgat gccactgccc ggcgcgcggc ggcggcgatg
      421 tatctggtcg gccggatctt cgccagcggg gcgcggctct atctggcggc gatcgcggtc
      481 tcgatgatcc tgttcctgga tgtgacgccg caacacatcg ccatagcggc ctttgccctg
      541 ctggtctttg ggctggtctt caccttcatg ggcgggctca attcggtgat ctggagcgat
      601 ctggtccagg tgacgctcta tgtcggggcg gcgctgctgg tcgcctggca cctgtggagc
      661 gggctcgacc tgaccgtggc cgaagccgtg accgcgctgc gccacacgcc cgacgggacc
      721 gacaagctgg cgctgatcaa ccccacgctg gacctttccg cgccgtgggg cctgtttgcg
      781 gtgctgaccg gggtcatgct gctcaacctc gccagttcgg ggctcgacca ggacatcacc
      841 cagcgcctgc tggcctgcga ggacgcgaag aagggcgcgc gctcgctcta tgtctcggtc
      901 tggatgagcc tgccggtgat cctgctgttc ctggtgatcg gatcgctgct gtatctgcac
      961 tatcagggca gcgcggcggg caccttccgg ggcgagaagg tgaccgtctt catgcattac
     1021 atcctgaccg agatcccgcc cgggctgcgc gggctgatca cggtgggggt gatcgcggca
     1081 gcggcgatca attcggggct gatctcgatg tcggccgtgc tggtcaacga tctctaccgg
     1141 ccctttgccg gcgtccgaac cgaggcgcat ttcgtgcgga tgggccggat cgccagcgtg
     1201 gtgctgggcc tggcgctctt cgccatggcg atcctgtcct attactggca gcgctatgcg
     1261 gattcggccc tgctcgattt cgtgctgggg gtgatggcct tcgcctattc cgggctgatc
     1321 ggggtttacg gcgtggcgct gttcagccgc cggggatcct cggccagtgt gatcgccgcc
     1381 ttcgtggccg ggttcctgac cgtcctggcc tttcagccct atgtgatcga cagcctgggc
     1441 ctgcccgccg cgctgaaggg cgtggccttt ccctggcagc tggtgctggg cagcgcggtc
     1501 gccgcactgg tctgcctcag cgcccccggc aagggagtgg cgcaagccgc cgccagtcgc
     1561 taaggcgctc tgcgatgaac accggcactt tcctgtccca ttccgccctt gtcgcccttg
     1621 gcggcgggat cggcgcctgg ctgcgctttg ccgccggccg cctgatcggc gcgggcgcct
     1681 tcccctgggc caccctgtcc gtcaacctga tcggcagcct ggcgatgggc ctgctggccg
     1741 gctggctggc ccgccacggt cagggcggcg agcaatggcg gctgctgctg ggcgtgggcg
     1801 tgctgggcgg cttcaccacc ttttccgcct tcagcctgga actggtgaac ctgatccagc
     1861 gcggccaggg cggcatggcc gcgctctatg ccgccgtttc ggtgatcggc gggatcgccg
     1921 gcctgtttgc gggcctggcc ctggcaagga ctttcgcatg accgagcgcg gcatcattcc
     1981 ggccgatacg gtccgccagt tcacggtcgc cgccgacgat gacggcgtgc ggctggaccg
     2041 ctggttcaag cgccacttgc cgcaagtcgg ctttgccacg gtcagccgct gggcccgcac
     2101 cgggcagatc cgggtcgacg gcaagcgcgc cgacgtggcc gaccggctga gcgcgggcca
     2161 ggtggtccgc gtgccgccgg gcggtgacag cccggacaag cagacccgcg cccgcaagcc
     2221 gctgaccgaa gagcagatcg ccctggccga gggcatggtc atttcgaagg accgcgccgc
     2281 gatcgtgctg aacaagccgc cgggcctggc gacgcagggc ggcagcggca cgttccagca
     2341 cgttgacggc ctgctcgatg cctatgccga cgatggcccg cgcccgcggc tggtccaccg
     2401 gctcgacaag gacacctccg gcgtcctcct gatcgcgcgc acgccgggca gcgcggccta
     2461 tttttccaag cgcttttccg ggcgctcggc ccggaagatc tactgggcgc tggtgatcgg
     2521 cgtgcccgat atcgacgacg gcgtgatcga gctgccgctg gccaagcagc ccggcacggg
     2581 cggcgagaag atgatggtcg acaagagcgg cgaagggcaa agtgcgcgca gccgctaccg
     2641 cgtgatcgac cgggccggca accgcgccgc ctgggtggaa ttgcagccgc tgaccgggcg
     2701 cacccaccag ctgcgcgtcc acatggcggc gatcggccat ccgatcgtgg gggacggcaa
     2761 gtacggcggg cccgacgcct tcctgtccgg cagcatcagc cgcaagatgc accttcacgc
     2821 ccgccggctg atcatcgacc atcccgatgg cgacatgatc gacgtgcgcg ccgacctgcc
     2881 cgagcatttc gccgccagcc ttgagcagct gggctttgac gaggccgatg gcgacgcgat
     2941 ggagctggtg ctggccaagc ctgccccggc caaagaagac aagaaggccg cggccaaggc
     3001 ccatgccaag cagatccgca aggagcgccg gggtgaacgc cgcagccgcg ccggcggggc
     3061 cagcccggtc cgcaagccgc cgaccaagcg cgggccgacc aagggcaagg gcagccccaa
     3121 gcccggcgca ccgaagaagc gctgatgcat cccaacgccg ccttccgcca tcccgaccgg
     3181 gacctgctgg agacgctgat cgaccagatc gggttcggca tggtctttgc ccagacgccc
     3241 gatggcccgc gcgtggccca tgtggcgctg cattcgaccg gcgacggcgc ggtccagttc
     3301 cacatctcgc gcgggaacgc cctggcgaag catctggcag gcagcaccgc gctggtcgtg
     3361 gtcaacggcc cggacggcta tgtcagcccg cgctggtatt ccgatccggc ccaggtgccg
     3421 acctggaact atgtcgcgct ggaactggaa ggccgggtcc ggcgaatgga cgccgaggcg
     3481 accctggcgc tggtcaccga cctgtcggac aagcacgagg cgcgggtcgc cgaaggcacg
     3541 ccctggacca tggacaagct gccgccggaa aagctgcgcg gcctgctggc cgggatcgtc
     3601 ggctttgaac tggaagtgca ggcctggcgc gaaaccgtga agctgagcca gaacaaggat
     3661 gcggccgagc gcgagcggct ggccgcgggg ctggaaagcg aaggcgcggt cggcattgcc
     3721 cagttgatgc ggaccctggc gaagtgacct tgcggctggc cctgttcgat tgcgacggca
     3781 ccctggtcga tggccaggcc ggggtggtga acgcgatgga ggcggccttt gccgccgtcg
     3841 gcctggcccc gccggaccgg caccaggtgc gccgggtggt cggcctcagc ctgccgcagg
     3901 cgatccgcct gctggcccct gccgcgccgg acggccagcg caccgccgcc gatgccgctt
     3961 accgcgaggc gttccgcacc gcgcgcgaat ccggtgcact ggttgagccg ctctacgacg
     4021 ggatcgccgc gctggtggcc cagctggacg aggccggctg gctgctcggc gtggcgaccg
     4081 ggaagagcga gcgcgggctg aaccactgcc tggccaccca cggcctgtcg gcccggttct
     4141 gcacgctgca gaccgccgat caccacccgt ccaagccgca cccctcgatg gtcgaggccg
     4201 cgctggccga aaccggcgtg gagcgcgccc ggtgcgtgat gatcggggac acccagttcg
     4261 acatggccat ggccgtcaac gccggggtcc gcgcgctggg ggtcgactgg ggctatcaca
     4321 cc
//
LOCUS       DASUDV010000537         3478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_261295,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000537 DASUDV010000000
VERSION     DASUDV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261295"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFV30_11785"
     CDS             complement(<1..677)
                     /locus_tag="VFV30_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX4848817.1"
                     /translation="MLAAERGAAANTLAAYRRDLEGAQEAIGDLVEADGPALASLGAA
                     WAGLAPSSLARKCSALRQFFGFLVDEGWRGDDPSPALPRPRTRRPLPRLLSHAEVEAL
                     FLRAEDEAAGEKPEAVRMLALLELLSGSGLRATELVSLPLSAVPRDAPFLTVTGKGGQ
                     QRMVPVSTRARAALSRWLALRGDGGKLLFPSTGRQGHLSRVRLFQLLRALAARAGIDP
                     EKVSPHVL"
     gene            complement(837..2654)
                     /locus_tag="VFV30_11790"
     CDS             complement(837..2654)
                     /locus_tag="VFV30_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848818.1"
                     /translation="MKRLLLLGAGTALALSSAWVAAQDSPESLLPPGFEKPKARPAAP
                     TPGATPVVQPLPGEGGTAAKTPTAPRTLPNGQRIPTLKELEAMTPDQLDELLGLKPKF
                     DMPAAARRSLKRIGILAEDEGGFPAGSLGKQDPRLVTAILDGNKGRLVSRWGHIVLRR
                     ALVSRLDAPAGMNPADFVGSRAGLLVRMGEGHLARAVVQDVDAGNYTENLTKAAIDAY
                     AFTGDITGICPVTTVIGGTRKDADWRVLRAICASFQGDGAAGMAQLDRLADEKAWPAV
                     DILLAQKYAGAAGKIRRAVKIEWDGVNDMNPWRYSLALAVGVQPPAELTKDLPVRYAW
                     ATATAPMAGLEARAAAADRAGAAGILSSTAMVDLYSQIYAQQDIKGDWAANAESLRSA
                     YVGATPSDRMAAMRTLWDGAADPEARYARQVLTAYAAARMPVSGDFAADAPDLIASML
                     AAGLDANAMRWAPQVDTGSLGWGMLVLAAPDRSQPVDSGAIDSFYGNDPSDEYRKTQF
                     LVAGLAGLGRIDPATAKDFGGKLDFDLDRRTRWSALIEQAADLNNRELVALLAGMGMQ
                     GSGWDKMTPLHLYHIVSALNRVGLSAEARMIAAEAVARG"
     gene            2860..3435
                     /locus_tag="VFV30_11795"
     CDS             2860..3435
                     /locus_tag="VFV30_11795"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX4848819.1"
                     /translation="MDHDAAMLSQPEIEALARRIDRPVVLVGLMGVGKSSIGRRLSAA
                     LHIPFVDADEEIERAAQMSISEIFAQFGEPYFRDGERRVIARLMEGPGNRRIIATGGG
                     AFVNAETRALILDKAIAVWLDSDVDVLVERVGRKDTRPLLRGGNPRDILARLKAEREP
                     AYAQAPIHVKSTAGPQGRTVAKVLKGIAEWL"
BASE COUNT          520 a         1275 c         1191 g          492 t
ORIGIN      
        1 agcacgtggg ggctgacctt ttccggatcg atcccagccc gcgccgccag cgcgcgcagc
       61 agctggaaca gccgcacgcg cgaaaggtgc ccttgccgcc cggtggaggg aaacagcagc
      121 ttgcccccgt cgcccctcag cgccagccag cgcgacagcg cggcccgcgc gcgggtggag
      181 accggcacca tccgctgctg cccgcccttg ccggtcacgg tcaggaacgg cgcgtcgcgc
      241 ggcaccgcgc tcaatggcag cgagaccagc tcggtcgcgc gcaggcccga tccggagagc
      301 agttcaagca gggccagcat ccgcaccgcc tcgggctttt ccccggcggc ttcgtcttcg
      361 gcgcgcagga acagcgcctc aacctcggcg tggctgagca ggcgcggcag cgggcggcgg
      421 gtgcgcgggc gcggcaatgc gggcgaggga tcgtcccccc gccagccctc atccaccaga
      481 aagccgaaga actgccgcaa ggccgagcat ttgcgggcca ggctggacgg ggcgaggccc
      541 gcccaggccg cgccgagcga ggccagagcc ggtccatcgg cctccaccag atcgccgatc
      601 gcttcctgcg cgccttccag atcgcggcga taggccgcca gggtgttggc cgccgcgccg
      661 cgttccgccg ccagcatggc aagaaatgaa tctatggcgt tcacgccatt ccctcttttc
      721 ccctcccgct tgcgggaggg gcaaggggag ggcctgtgtt ccgcttggcc gcaacatgcc
      781 ctcccccaac ccctcccgca agcgggcggg gagagacaga gagcttcgcg cgcctgttac
      841 ccccgcgcca cggcctcggc cgcgatcatc cgcgcctcgg ccgaaagacc gacgcggttc
      901 agcgccgata cgatgtgata gaggtgcagc ggggtcatct tgtcccagcc gctgccctgc
      961 atgcccatgc ccgccagcag cgccaccagt tcgcggttgt tgagatcggc ggcctgctcg
     1021 atcagcgccg accagcgggt ccgccggtcc agatcgaagt ccagcttgcc gccgaagtcc
     1081 ttggccgtgg ccggatcgat ccggcccagc ccggccaggc ccgccaccag gaactgggtc
     1141 ttgcggtatt cgtcgctggg atcgttgccg tagaagctgt cgatcgcgcc gctgtccacc
     1201 ggctggctgc ggtccggcgc ggccaggacc agcatccccc agccgagcga gccggtatcg
     1261 acctgcgggg cccagcgcat ggcgttcgcg tccagcccgg cggccagcat cgaggcgatc
     1321 agatcgggcg catcggcggc gaaatcgccc gaaaccggca tccgcgcggc ggcataggcg
     1381 gtcagcacct ggcgggcata gcgggcctcg ggatcggccg cgccgtccca cagggtccgc
     1441 atcgcagcca tccggtcaga cggagtggcg ccgacatagg cgctgcgcag cgattccgcg
     1501 ttggcggccc aatcgccctt gatgtcctgc tgggcataga tctggctgta gagatcgacc
     1561 atcgccgtgc tggagaggat ccccgcggcc ccggcccggt cggccgcggc ggcgcgcgct
     1621 tccaggcccg ccatcggcgc ggtggcggtg gcccaggcat agcggaccgg caggtccttg
     1681 gtcagttcgg ccggcggctg caccccgacc gccagggcca ggctgtagcg ccaggggttc
     1741 atgtcgttga ccccgtccca ttcgatcttc accgcgcggc ggatcttgcc cgccgctccg
     1801 gcgtatttct gggccagcag gatatcgacc gcgggccagg ccttttcatc ggccagccgg
     1861 tccagctgcg ccatcccggc cgcgccatcg ccctggaacg aggcgcagat cgcccgcagc
     1921 acccgccagt cggcatcctt gcgggtcccg ccgatcaccg tggtaaccgg gcagatcccg
     1981 gtgatgtcgc cggtaaaggc ataggcgtcg atcgccgcct tggtcaggtt ttcggtgtag
     2041 ttgccggcat cgacatcctg caccaccgcg cgggccaggt gcccctcgcc catccgcacc
     2101 agcagaccgg cgcggctgcc gacgaagtcc gccgggttca tcccggccgg ggcatccagc
     2161 cgcgaaacca gcgcgcggcg cagcacgatg tgcccccagc gcgagaccag ccggcccttg
     2221 ttgccatcga gaatcgcggt gaccagccgg ggatcctgct tgcccagcga tccggccgga
     2281 aagccgccct cgtcctcggc caggatcccg atccgcttga gcgagcggcg cgcggcggcc
     2341 ggcatgtcga acttgggctt caggcccagc agctcgtcca gctggtcggg cgtcatcgcc
     2401 tccagctcct tcagcgtcgg gatgcgctgg ccgttgggca gggtccgcgg cgcggtcggc
     2461 gtcttggcgg cagtgccccc ttcgccgggc aagggctgga ccacgggggt cgcccccggc
     2521 gtcggcgcgg cgggccgggc cttgggcttt tcgaaaccgg gcggcagcag cgattcgggc
     2581 gaatcctgcg cggcgaccca ggccgaggac agcgccagcg cggtgccggc gcccagcagc
     2641 agcaagcgct tcattgcccg ccccccggca gcatggccgg cgcggactgc gggccgaccg
     2701 ggcgctctcc gccgtcggac caggcccagc ccagcaccgc caccagcgcg gcccctgcca
     2761 acaggatcat tccagacttg cgcctatcca acgccacgcg cgacctctcc tgcccgaaaa
     2821 cttgcaacgc gggttagcct atctataggc ggggccgcaa tggaccatga tgcagccatg
     2881 ctttcccagc ccgaaatcga ggcgctggcc cggcggatcg accgcccggt cgtgctggtc
     2941 ggcctgatgg gcgtgggcaa atcgagcatc ggccgccgcc tctccgccgc gctgcacatc
     3001 ccctttgtcg acgccgacga agagattgag cgcgcagcgc agatgtccat ttccgaaatc
     3061 ttcgcccagt tcggggaacc ctatttccgc gatggcgagc ggcgggtgat cgcccggctg
     3121 atggaagggc ccggcaaccg ccggatcatc gccaccggcg gcggggcctt cgtcaacgcc
     3181 gaaacccggg cgctgatcct ggacaaggcg atcgccgtct ggctcgacag cgatgtcgac
     3241 gttctggtcg aacgggtcgg gcgcaaggac acgcgcccgc tgctgcgggg tggcaacccg
     3301 cgcgacatcc tggcccggct gaaggcggaa cgcgaaccgg cctatgccca ggcgccgatc
     3361 catgtgaaaa gcacggccgg gccgcagggc cgcaccgtgg ccaaggtgct gaaaggaatt
     3421 gccgaatggc tgtgatcccg gtcgacatcg cgggcagccc ctacgaggtg cggatcgg
//
LOCUS       DASUDV010000538         7760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1410634,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000538 DASUDV010000000
VERSION     DASUDV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410634"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFV30_11800"
     CDS             <1..212
                     /locus_tag="VFV30_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848820.1"
                     /translation="LDDLQRQGQQTAWFWGGQIGGIVVLMALVAAAGRDSDLARGGLY
                     VVLGQAVGFAVAWLVWRWRSSGPAE"
     gene            209..406
                     /locus_tag="VFV30_11805"
     CDS             209..406
                     /locus_tag="VFV30_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4848821.1"
                     /translation="MKNRLRELRRERGLNQADLAEQLQVSRQTIIAIEADKYDPSLPM
                     AYRLAAFFDIPVEDLFFNPYR"
     gene            complement(416..919)
                     /locus_tag="VFV30_11810"
     CDS             complement(416..919)
                     /locus_tag="VFV30_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX4848822.1"
                     /translation="MSEFSRPYDAARLPADEQVLDASPAECAALAQRFALVAVNTLTA
                     KIALKADGPTVRAAGRVQADIVQSCAVSGEDLAVRIDEPLTLRFVPSGESPAGDEEIE
                     LAAEELDDIEMENGRFDLGEAVAQTLALAIDPYLEGPNAEEFRRKAGILSEGEGGAFA
                     ALKNLLK"
     gene            complement(916..1446)
                     /locus_tag="VFV30_11815"
     CDS             complement(916..1446)
                     /locus_tag="VFV30_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX4848823.1"
                     /translation="MSLLQRLLGRPDPGQEQVRPLWHRVVELSRDPDWYRPAPDGGGI
                     ADTVPGRFDAITLVLSLVMLRMEGADALRAPSARLTELFVKDMDGQLRQSGVGDLVVG
                     KKVGKLMGALGGRIDSFREALAQPDDAALIAAIERNVTLSEDGNPAAIATRMRALAGR
                     LAAIDSADLLAGTIPA"
     gene            1599..2048
                     /gene="bamE"
                     /locus_tag="VFV30_11820"
     CDS             1599..2048
                     /gene="bamE"
                     /locus_tag="VFV30_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX4848824.1"
                     /translation="MPIKGRILAVALAAMTLSACATIRDQRGYLVDGALLDSIQPGVD
                     NQLSIERMLGRPSFVSQFGRQDWYYISTQTKQAAFTRPKPSQQLLVKISFDDKGNVAS
                     VERSGMEKVANINPDGDKTPTLGRERTFLEDLFGNIGQVGAAAPAGN"
     gene            2098..2655
                     /gene="hslV"
                     /locus_tag="VFV30_11825"
     CDS             2098..2655
                     /gene="hslV"
                     /locus_tag="VFV30_11825"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX4848825.1"
                     /translation="MSTSRDSHGLVQWHGTTIIGVKKGGKTVIAGDGQVSMGNTVMKP
                     NARKVRRLGDGSVIAGFAGATADAFTLFERLEKKLEATRGQLMRAAVELAKDWRTDKY
                     LRNLEALMIVADKDVLLILTGNGDVLEPEEGIAAIGSGGNYALSAAKALTDYEDDPEK
                     IARRAMAVAADVCVFTNDRVTVEVI"
     gene            2661..3962
                     /gene="hslU"
                     /locus_tag="VFV30_11830"
     CDS             2661..3962
                     /gene="hslU"
                     /locus_tag="VFV30_11830"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX4848826.1"
                     /translation="MSENLTPKAIVRALDEHIVGQADAKKAVAVALRNRWRRQRLSPE
                     LRDEVTPKNILMIGPTGCGKTEISRRLAKLAEAPFVKVEATKFTEVGYVGRDVEQIAR
                     DLVEEAIRLEKERRREAVRDAAEKGAMDRLLKALVGDGASEATRESFRQRIAEKAMDD
                     IEVEIEVEDSPAGGMEIPGMPGGIGMINLGDMMSKAMGKQNLKRRKLKVPDAWAKLVD
                     EEAEKRMDQDDVARVALANAETNGIVFIDEIDKIAVSDVRGGSVSREGVQRDLLPLIE
                     GTTVATKYGPMKTDHVLFIASGAFHVAKPSDMLPELQGRLPIRVELKALSEEDFVRIL
                     SETRANLVEQYKALLATEKVTLDFAPGAVREVAKIAAQVNEAVENIGARRLQTVMEKL
                     LEELSFEAEDREGETVTVDEAYVRERLSGLAKDTDLSKYIL"
     gene            3972..4145
                     /locus_tag="VFV30_11835"
                     /pseudo
     CDS             3972..4145
                     /locus_tag="VFV30_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            4188..4391
                     /locus_tag="VFV30_11840"
     CDS             4188..4391
                     /locus_tag="VFV30_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HEX4848827.1"
                     /translation="MKCQTPDDKPIYRLLTGKDDRAFCERVSEALEQGWRLYGSPSLA
                     WDEGHLCMKVAQAVVWHEADVVK"
     gene            complement(4396..4950)
                     /locus_tag="VFV30_11845"
     CDS             complement(4396..4950)
                     /locus_tag="VFV30_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848828.1"
                     /translation="MNQLGLDLTKASYWTEFDEQGERLVMPIKRSWKTVGWAVAAFGF
                     ALVKMHAVFEHEPAAFEYMVFIAIIAAALYLVFSIVTSLLAREVIQIRHGDLVHGWRL
                     LGLKREKAYRLGDITHLRVGRLDSEAGLDKLVSPLKDFGKAGVVAFEHSTGTVWLGAA
                     LDQAHGQQVVDWIARRAPRGILAA"
     gene            complement(4998..6830)
                     /locus_tag="VFV30_11850"
     CDS             complement(4998..6830)
                     /locus_tag="VFV30_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX4848829.1"
                     /translation="MGPMHAPATPAPDTATRHDSWATLRRFLPYLWPKDRPALKRKIV
                     LAVLLVLASKAVTLALPFAYKHAIDAMKVPGTAAFQIAFAMVLAYALGRFSGVVFDNL
                     RNIVFERVGQEATRQLAVDTFNRLHQLSLRFHLARRTGEVTKTIDRGTKSIDVMLYFM
                     LFNIAPTVIELTAVAIIFYLSFGWGLVMATAVAVVAYGAVTRWITDWRNALREKMNRL
                     DGQALARSVDSLLNYETVKYFNAEAREAKRYEQAAQDFANAAVKSENSLGLLNIAQAL
                     ITNLLMAGAMAYTVWGWLQGRHTVGDLVFVQTYLMQLFRPLDLLGMVYRTIRQGLIDM
                     AQMFELIDTPVEVADAPGAPALVVNRPTLTFDNVVFGYEPDRTILNGLSFEVPAGHQV
                     ALVGPSGAGKSTIARLLFRFYDPQAGRILIDGQDIRAVTQSSLRAAIGIVPQDSVLFN
                     DTIGYNIAYGRDGAGPAEVEAAARGAALMDLIGRLPQGFDTEVGERGLKLSGGEKQRV
                     AIARTLVKNPPILLLDEATSALDSRTEQDILATLHAVSAGRTSLTIAHRLSTIADADT
                     ILVLSEGRLAEQGTHQQLLRKRGLYAEMWARQAEAVEEFAEPAR"
     gene            complement(6886..>7760)
                     /locus_tag="VFV30_11855"
     CDS             complement(6886..>7760)
                     /locus_tag="VFV30_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX4848830.1"
                     /translation="LMLSLPVLAGQEGSGKAGEVDVVEIGSDRFDRMTVPVRIGAHGP
                     FDFMIDTGAQNTVISTSLATRLALPTAKRVRLTGIAGTKVVDSVEIEQIDLGTRSYYG
                     VLAPLLEKKDIGADGIIGLDSLQGQRVQIDFKKGTMAVADAKTLGGNRGYDIVVTARR
                     RSGQLIMAEAKIDGIRVDVVIDTGAESSIGNRALQKALARRHRGEQTELLSVTGQTIT
                     ADINYASRLTIGEMSFTNVMIAYADSPPFEILGLSKRPALFLGMRDLRQLDRLAIDFA
                     ARKVFFDLPPSAFH"
BASE COUNT         1338 a         2503 c         2646 g         1273 t
ORIGIN      
        1 ggctggacga tctgcaacgc cagggccagc agaccgcgtg gttctggggc ggccagattg
       61 gcgggatcgt ggtcctgatg gccctggtgg ccgcggccgg ccgcgacagc gatctggcgc
      121 gcggcggcct ttatgtcgtg cttggccaag cggtgggttt cgcggttgcc tggctggtct
      181 ggcgttggcg cagcagcggg ccggctgaat gaagaaccga cttcgcgaat tgcgccgcga
      241 acgcggtctc aaccaggccg atctggccga gcagttgcaa gtttcccgcc agacgatcat
      301 cgcgatagag gcggacaagt acgacccttc gctgccgatg gcttaccgat tggccgcctt
      361 tttcgacatt ccggtcgaag acctgttctt caacccctac cgctaagcgc gttggctatt
      421 tcagcaggtt cttcagcgcg gcaaaggcgc cgccttcgcc ctcgctgagg atgccggcct
      481 tgcggcggaa ttcctcggcg ttggggcctt ccagataggg gtcgatggcc agggccaggg
      541 tctgggccac ggcctcgccc agatcgaacc ggccgttctc catctcgatg tcgtccagct
      601 cttcggcggc gagttcgatt tcctcgtcgc cggccgggct ctcgcccgag gggacgaagc
      661 gcagggtcag cggctcgtcg atccgcaccg ccagatcctc gcccgatacg gcgcagctct
      721 ggacgatgtc ggcctgcacc cggcccgcgg cgcggacggt cggcccgtcg gccttgagcg
      781 cgatcttcgc cgtcagcgtg ttgaccgcca ccagggcaaa ccgctgtgcc agcgccgcgc
      841 attcggccgg cgatgcgtcc agcacctgtt cgtccgcggg caggcgggcg gcgtcatagg
      901 ggcgggaaaa ctcgctcatg ccgggatcgt tccggccagc aggtcggcgc tgtcgatcgc
      961 ggccagccgg ccggccagtg cgcgcatccg ggtggcgatc gccgccgggt tgccgtcctc
     1021 gctcaaggtg acattgcgtt cgatcgcggc gatcagcgcg gcgtcgtccg gctgggccag
     1081 cgcctcacgg aatgaatcga tccggccgcc cagcgcgccc atcagcttgc cgaccttctt
     1141 gcccaccacc agatcgccca cgccgctttg ccgcagctgg ccgtccatgt ccttgacgaa
     1201 cagttcggtc agccgggccg agggcgcgcg cagcgcatcg gcgccttcca tccgcagcat
     1261 caccaggctg agcaccaggg taatcgcatc gaaccggccg gggaccgtat cggcgatccc
     1321 gccgccgtcc ggggcagggc ggtaccagtc cggatcgcgc gacagttcga ccacgcggtg
     1381 ccacagcggg cggacctgct cctggccggg atcgggcctg ccgagcaggc gttgcagcag
     1441 cgacattgcg gggtcgtcct tccattcagc gggcattaaa ccgggcccgc ccacccaccg
     1501 ccattgcgcg gcgggccaag gcggcctaag gctggccgcg atagatcata ggggcggcgc
     1561 gggcaatccc ggggtgccgc cggacggagc aagtactgat gccgatcaag ggacgaattc
     1621 tggctgtggc gctggcggca atgacgcttt cggcctgcgc aaccatccgg gaccagcgcg
     1681 gttatctggt cgacggtgcg ctgctcgatt cgattcagcc cggggtcgat aaccagctgt
     1741 cgatcgaacg catgctgggc cggcccagct tcgtcagcca gttcggccgc caggattggt
     1801 actatatctc tacccagacc aagcaggccg ccttcacccg cccgaagccg agccagcagc
     1861 tgctcgtcaa gatcagcttt gacgacaagg gcaatgtcgc ctcggttgag cgttcgggca
     1921 tggaaaaggt cgccaacatc aaccccgatg gcgacaagac gccgaccctg gggcgcgagc
     1981 ggaccttcct ggaagatctg ttcggcaaca tcggccaggt cggcgcggcc gctcccgccg
     2041 gcaactaggc tgcccggcca aggttgaagc gcgcgggata gtccatatat cccgtgcatg
     2101 agcacttcac gcgacagcca cggactggtc cagtggcacg gcaccaccat catcggcgtc
     2161 aagaagggcg gcaagaccgt cattgccggc gatggccagg tttccatggg caacacggtg
     2221 atgaagccga acgcgcgcaa ggtgcggcgg ctgggcgatg gctcggtcat cgccgggttc
     2281 gccggggcca ctgccgatgc cttcaccctg ttcgaacggc tggaaaagaa gcttgaggct
     2341 acccgcggcc agctgatgcg cgcggcggtg gaactggcca aggactggcg caccgacaag
     2401 tacctgcgca acctggaagc cctgatgatc gtggccgaca aggacgtgct gctgatcctg
     2461 accggcaacg gcgacgtgct ggaacccgaa gaggggatcg cggcgatcgg atcgggcggc
     2521 aactatgccc tgtccgctgc caaggcgctg accgattacg aggacgatcc cgaaaagatc
     2581 gcccgccgcg ccatggcggt tgccgccgat gtctgcgttt tcaccaatga ccgcgtgacg
     2641 gttgaggtca tctgaacccc atgagcgaaa atctgacccc caaggcgatc gtccgcgcgc
     2701 tggacgagca catcgttggc caggccgatg ccaagaaggc tgtggccgtg gccctgcgga
     2761 atcgttggcg gcgccagcgc ctttcgcccg agctgcgcga cgaggttacg cccaagaaca
     2821 tcctgatgat cggcccgacg ggctgcggca agaccgagat cagccgccgc ctggccaagc
     2881 tggccgaggc tccgttcgtg aaggtcgagg cgacgaagtt caccgaggtc ggctatgtcg
     2941 gccgcgatgt cgagcagatc gcgcgcgacc tggtcgagga agcgatccgg ctggagaagg
     3001 agcgccggcg cgaggcggtg cgcgatgcgg ccgagaaggg cgcgatggac cggctgctga
     3061 aggcgctggt cggcgatggc gcttcggaag cgacgcgcga atcgttccgc cagcggatcg
     3121 ccgaaaaggc gatggacgac atcgaggtcg agatcgaagt ggaagacagc ccggccggcg
     3181 gcatggagat ccccggcatg cccggcggga tcggcatgat caatctgggc gacatgatgt
     3241 cgaaggcgat gggcaagcag aacctgaagc gccgcaagct gaaggtgccc gatgcctggg
     3301 ccaagctggt cgacgaagag gcggaaaagc gcatggacca ggacgatgtc gcccgggtcg
     3361 cgctggccaa tgccgaaacc aacggcatcg tcttcatcga cgagatcgac aagatcgccg
     3421 tgtccgacgt gcgcggcgga tcggtgtccc gcgaaggggt gcagcgcgat ctgctgccgc
     3481 tgatcgaagg caccacggtg gccaccaagt acggcccgat gaagaccgac cacgtgctgt
     3541 tcatcgcctc tggcgcgttc cacgtggcca agcccagcga catgctgccc gaactgcagg
     3601 ggcgcctgcc gattcgggta gagctgaagg ctttgtcgga agaggacttt gtccggatcc
     3661 tgtccgaaac ccgcgcgaac ctggtcgaac agtacaaggc gctgctggcg accgagaagg
     3721 tcacgctcga cttcgccccc ggcgcggtcc gcgaagtggc gaagatcgcg gcgcaagtga
     3781 acgaggcggt cgagaacatc ggcgcgcggc ggctgcagac cgtgatggaa aagctgctgg
     3841 aagagctgag cttcgaagcc gaggaccgcg agggcgagac cgtgaccgtg gacgaagcct
     3901 atgtccgcga gcggctgagc gggctggcca aggacacgga tctgtcgaag tatattctct
     3961 aaggagggtt tgtgtctgac ctgctccgga tcaagcagtt gcgcgcaaat gcgggcctga
     4021 cacaggctga ccttgccgct gccatgggcg ttgagcagcc tacgatccat cgatgggaaa
     4081 atggaaagca ggaaccttcg atcaagagtt tgatcgactt ggccaaagcg cttggcgttt
     4141 cggttggggg cctcttctct gagaacgtcg ctggttcaag tagcagtctg aagtgtcaaa
     4201 cccccgacga caagccgatc taccgcctgc tcaccggcaa ggacgaccgc gccttttgcg
     4261 agcgcgtgtc cgaagcgctg gagcagggct ggcggcttta cgggtcgccc agcctggcct
     4321 gggacgaagg acacctctgt atgaaggtcg cccaggccgt ggtctggcac gaagccgacg
     4381 tggtgaagta gcccctcagg ctgccaggat gccgcgcggg gcgcggcggg cgatccagtc
     4441 caccacctgc tggccgtgcg cctggtccag cgccgcaccc agccagaccg tgccggtgct
     4501 gtgttcgaag gcgacgacgc ccgccttgcc gaaatccttg agcggcgaaa ccagcttgtc
     4561 gagcccggct tcgctgtcca gccggccaac gcgcaggtgc gtgatgtcgc ccagccggta
     4621 ggccttctcg cgcttcagcc ccagcagtcg ccagccgtgc accagatcgc cgtggcggat
     4681 ctggatgacc tcgcgggcca gcaggctggt gacgatgctg aacaccagat agagcgccgc
     4741 ggcgatgatc gcgatgaaga ccatgtattc gaacgcggcg ggctcgtgct cgaacacggc
     4801 atgcatcttg accagggcaa agccgaaagc ggccacggcc cagccaaccg tcttccagct
     4861 gcgcttgatc ggcatgacca gccgttcgcc ctgttcgtcg aactcggtcc agtaggacgc
     4921 cttggtcaga tcgaggccga gttggttcat ggcgccaagc ctatgccccg gttccggccc
     4981 ggtcaatcgg gcgcaagtta ccgcgccggc tcggcaaatt cctcgaccgc ctcggcctgg
     5041 cgggcccaca tttcggcata gagcccgcgc ttgcgcagaa gctgctggtg cgtgccctgt
     5101 tcggccagcc ggccttcgct cagcaccagg atcgtgtcgg catcggcaat ggtcgaaagg
     5161 cggtgggcga tggtcagcga cgtgcggccg gccgaaacgg cgtgcagggt ggccaggata
     5221 tcctgctcgg tccggctgtc gagcgcgctg gtcgcctcgt ccagcagcag gatcggcggg
     5281 ttcttgacca gggttcgggc gatggcgacg cgctgctttt ccccgcccga cagcttgagc
     5341 ccgcgctcgc ccacttcggt gtcaaagccc tgcggcaggc gcccgatcag gtccatcagc
     5401 gccgccccgc gcgccgcagc ctcaacctcg gccgggccgg cgccgtcgcg gccataggcg
     5461 atgttgtagc cgatggtgtc gttgaacagc accgaatcct gcggcacgat cccgatggcg
     5521 gcgcgcaagc ttgactgggt gacggcgcgg atgtcctggc cgtcgatcag gatccggccg
     5581 gcctggggat cgtagaaacg gaacagcagc cgggcgatcg tgctcttgcc cgcgccggag
     5641 gggccgacca gggcgacctg gtgcccggcg ggcacttcga acgacaggcc gttgaggatc
     5701 gtgcggtctg gctcgtaccc gaacaccacg ttgtcaaagg tcagcgtcgg acggttcacc
     5761 accagcgccg gagcgcctgg cgcgtcggcg acttcgaccg gcgtgtcgat cagttcgaac
     5821 atctgggcca tgtcgatcag cccctggcgg atcgtgcgat agaccatgcc gagcagatcg
     5881 agcgggcgga acagctgcat cagataggtc tggacgaaga ccagatcgcc caccgtgtgc
     5941 cggccctgca gccagcccca cacggtatag gccatggccc cggccatcag caggttggtg
     6001 atcagcgcct gggcgatgtt gagcaggccc aggctgttct cgctcttcac cgcggcgttg
     6061 gcgaaatcct gggcggcctg ttcatagcgc tttgcctcgc gcgcttcggc gttgaagtac
     6121 ttcaccgtct cgtaattgag cagcgaatcg accgaccggg ccagcgcctg gccatcgagc
     6181 cggttcatct tctcgcgcag ggcgttgcgc cagtcggtga tccaacgggt caccgcgcca
     6241 taggcgacca ccgccacggc cgtggccatg acaagccccc agccgaagct gaggtagaag
     6301 atgatcgcca ccgcggtcag ctcgatcacg gtcggggcga tgttgaacag catgaaatag
     6361 agcatcacgt cgatgctctt ggtcccgcgg tcgatcgtct tggtcacttc gccggtgcgg
     6421 cgggccaggt gaaagcgcag cgagagctgg tgaaggcggt tgaacgtgtc gaccgccagc
     6481 tggcgggtgg cttcctgccc gacccgttcg aacacgatgt tgcgcagatt gtcgaacacc
     6541 acgccggaaa agcggcccag cgcataggcc aggaccatgg cgaaggcgat ctggaaggcg
     6601 gccgttcccg gcaccttcat cgcgtcgatc gcgtgcttgt aggcaaaggg cagggccagg
     6661 gtcacggcct tgctggccag gaccagcagc accgccagca cgatcttgcg cttcagcgcg
     6721 ggccggtcct tgggccacag atagggcagg aaccggcgca gcgtggccca gctgtcgtgg
     6781 cgggttgcgg tgtcaggtgc gggcgtggcc ggtgcgtgca tcggccccat gtggggcggg
     6841 gccgctcaaa agggaagggt caagcccgcg ccggtgcggg cagccctagt gaaaggcgct
     6901 gggcggcagg tcgaagaaca ccttgcgggc ggcgaaatcg atcgccagcc ggtccagctg
     6961 gcgcagatcg cgcattccca ggaacagcgc cgggcgcttg ctcagcccca ggatctcgaa
     7021 cggaggcgaa tcggcatagg cgatcatcac gttggtgaag ctcatttcgc cgatggtcag
     7081 cctgctggca taattgatgt cggcggtgat ggtctggccg gttacggaca gcagttcggt
     7141 ctgctcgccg cggtggcggc gggccagggc cttttgcagc gcgcggttgc cgatcgagga
     7201 ttcggccccg gtgtcgatca ccacgtcgac ccggatcccg tcgatctttg cctcggccat
     7261 gatcagctgg cccgagcgcc gccgcgcggt gacgacgatg tcgtagccgc ggttgccgcc
     7321 cagcgtcttg gcatcggcca ccgccatggt gcccttcttg aaatcgatct ggacgcgctg
     7381 gccctgcagg ctgtccagcc cgatgatccc gtcggcgccg atgtccttct tttccagcag
     7441 cggcgcgagg acgccgtagt aagagcgggt gcccagatcg atctgctcga tctccaccga
     7501 atcgaccacc ttggtgcctg cgatccccgt cagccgcacc cgcttggcgg tcggcagcgc
     7561 caggcgcgtg gccagcgagg tggaaatgac ggtgttctgc gccccggtat cgatcatgaa
     7621 atcgaacggc ccgtgcgcgc cgatccgcac cggcacggtc atccggtcga accggtcgct
     7681 gccgatttcg acaacatcga cttcaccggc tttgccgctg ccctcctgtc cggccagcac
     7741 cggcagcgac agcatcagcg
//
LOCUS       DASUDV010000539         6407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_858105,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000539 DASUDV010000000
VERSION     DASUDV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858105"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFV30_11860"
     CDS             complement(<1..585)
                     /locus_tag="VFV30_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX4848831.1"
                     /translation="MRFAAPLLALSLLAVPAIVVAGQAQSFDPEAVKAHVTFLADDTL
                     DGRGSGTKGYTIAANYVASQFRMIGAVPMGDPAKGSYFQQVPLREAKLVGQPVVTVTG
                     KGQTLRFDTREQVLIGPSAQDAQQSVSAPLVFAGFGIDRPELGLRDYAKLDVKGKIVV
                     VLSGYPKGLNSELGAHLSSDKARMAMNRGAVGVIT"
     gene            complement(758..1888)
                     /locus_tag="VFV30_11865"
     CDS             complement(758..1888)
                     /locus_tag="VFV30_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4848832.1"
                     /translation="MLNKKMLGLALAALPMVALASPAQAQAAGDKIANSYICVFNKSV
                     SRGQVESEANRSVKAHGGQVKHVYRVALQGFAADLPAAAVANMQRSNPNIAYCEQDQV
                     MAAPPIRIEARPGGGGSTQPPQETPWGIARVNGGGANYTGGRTAWIIDSGIDLTHPDL
                     NVDTARSASFLGGTTTPQDQNGHGTHVAGTIAACNNTIGVIGVTPCARVVAVRVLDRR
                     GSGSTSGVIAGVEYVAANGAPGDVANMSLGGGVSTTLDTAVVNASAGGVRFVLAAGNE
                     SDNANNHSPARANGPNVYTVSAFANGGGFASFSNYGNPPVDWAEPGVSIKSTWLSGGY
                     NTISGTSMAAPHLAGILLSGNVTANGTVSGDPDGNPDPIGVR"
     gene            complement(2103..3647)
                     /locus_tag="VFV30_11870"
     CDS             complement(2103..3647)
                     /locus_tag="VFV30_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX4848833.1"
                     /translation="MAAAVAVAAPVLLLAPQAPLRAQGAPIPAVLEELKPVTSDDGQA
                     AYMLRFSPKEPRIDPLNTNPTKPAIIIRNALRAPQVTPVFSGKGLVRSARFETAGQDL
                     VLRFDTSSPARVTVEPAGPNVLQIKVRKVSEEEATGSRPVGSEGAAVAPASTLSSQMT
                     VRPGESFELIPLKYADVSEVIGLLVEGSTIEPNNVFIRREPGFGSLGSNNQPQYLQMQ
                     TQQQAPAKQLGQSFGNGLGVDRRLNAIWVSGTPERIAQVKAQIALIDVPVDSVILETQ
                     FVELTETGAKNLGIDLNNSNGQIGIGKIETGTFLPFSIDPNDVLPSGVLQAALYAQIE
                     KGEGRILSRPRISAQSGSTAKIITGDALPILTSITLSGVNGVSQQVQYVNVGVTLQIA
                     PRVSPDGYVTAQIYGVVSSVTGYSQGYPTISQREAETSVTVRDGETFVIGGLTQENVL
                     KRKSKVPLLGDIPIAGALFRTERSTSSKTELYIVITPHIVRHRRFEAPPPPVDENGES
                     PSAPQQ"
     gene            3920..>6407
                     /locus_tag="VFV30_11875"
     CDS             3920..>6407
                     /locus_tag="VFV30_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX4848834.1"
                     /translation="MRRLAAKPLLASLLALSLTACSTGPAAELASTPAPASAAPAAKP
                     APQAAPLGELVKAVDIPYEKFTLANGLTVLVHTDRKAPIVGVTLYYRVGSKHEPKGRT
                     GFAHLFEHLMFGGSENVPNFDVPLEAAGSTPTNGSTWFDRTNYVETVPKGAVNLALFM
                     ESDRMGHLLGAVTQDKLEKQRGVVKNEKRQGDNEPYGLAEYAIGEGLFPVGHPYRHST
                     IGSMADLDAASMEDVRNWFRGNYAPNNVVLVLSGDIDAATARPMVEKWFGHIPRGPAV
                     AKVEAGPVTLAAPVKREMTDQVPLTRVYRVWSGPGLNDPDAPALDLGLKVLGGLSSSR
                     LDNALVRGKGVAVSVSAYVLQFEQTSYLQVQMDIKPGVDRAVAEAALDAEIEKLINEG
                     PTDDELRRAATTAVSGQIFALEQVGGFSGKGAQLAEGELYSGDPAQFKKDLERIAALT
                     PLDVKGALQRWMKRPVYALAITPGKRTEKGETMGGWGDEATSPPPAPDAGKPAPKLAT
                     GPKREFPPVEAVGDLDFPKVERATLSNGIPVVLARRSGVPTVLVNLTFDAGFAADARD
                     KPGTQTLLLSVLDEGTESRDATRIAEDQERLGARISTGTGIDSSTVTLSALSANLAPS
                     LDLMADVVRNPAFREADVARARDQQLAGIAQANASPSALANRSLGPILFGPAHPYGQP
                     SDGLGDARSVGSLTPAALRTAQAEWLRPDNLTITVVGDVSMEALLPMLEASFGTWQAP
                     ATPRGTKSLDAAIPAPRPRIVLIDRPGSPQSVIVAGRVLPLTGRDQGLEPLELANEVL
                     GNNFLSRLNLDLREEKGWSYGVRSVVRSPRG"
BASE COUNT         1011 a         2189 c         2182 g         1025 t
ORIGIN      
        1 cgtgatcacg cccaccgcgc cgcggttcat cgccatgcgc gccttgtcgg agctgaggtg
       61 cgcgcccagc tcggaattca ggcccttggg atagccgctg aggacgacga cgatcttccc
      121 cttcacgtcc agcttggcgt agtcgcgcag gcccagctcc ggccggtcga tgccaaaacc
      181 ggcgaagacc agcggcgcgg aaacgctctg ctgcgcatcc tgcgcactgg gcccgatcag
      241 cacctgctca cgcgtgtcga agcgcagggt ctggcccttg ccggtcacgg tcaccaccgg
      301 ctggccaacc agcttggcct cgcgcagcgg gacctgctgg aaatagctgc ccttggccgg
      361 atcgcccatc gggaccgcgc cgatcatgcg gaactgggac gcgacgtagt tggcggcgat
      421 cgtatagccc ttggtccccg aaccgcgccc gtccagggta tcgtcggcca ggaaggtgac
      481 gtgggccttg accgcttcgg gatcgaagga ctgggcctgt ccggccacga cgatggccgg
      541 aacggcaagg agtgacaggg ccagcaaggg cgcggcgaaa cgcatggact tctcccaggg
      601 gtttgtttgc ggcactggct agccgggccg gcgcggggac acaaggctga ccgaaagggt
      661 atgcgcctgc cgccgccagc caccccctgc cagcaatgaa aaggggcggc ccgccagccg
      721 gcggaccgcc cccttcgtta ccctgacggg tgtgcgatca gcgcacgccg atcggatccg
      781 ggttgccgtc cggatcgccc gagacggtgc cgttggcggt cacgttgccc gagagcagga
      841 tcccggccag gtgcggcgcg gccatcgagg tgcccgagat ggtgttgtag ccaccgctca
      901 gccaggtcga cttgatcgac acgcccggtt cggcccaatc gaccggcggg ttgccgtagt
      961 tcgagaagct ggcaaagccg ccgccgttgg caaaggccga aacggtgtag acgttcgggc
     1021 cgttggcgcg ggccggcgag tggttgttgg cgttgtcgct ttcgttgccc gcggcgagca
     1081 cgaagcgaac cccgccggcc gaagcgttga ccacggcggt gtcgagcgtg gtcgaaaccc
     1141 cgccgcccag gctcatgttg gcgacatcgc ccggcgcgcc gttggcggcg acatactcaa
     1201 ccccggcgat cacgcccgag gtgctgcccg agccgcggcg gtccagcacg cgcacggcca
     1261 cgacgcgggc gcagggggtg accccgatca caccgatcgt gttgttgcag gcagcgatcg
     1321 tgccggcgac gtgggtgccg tggccgttct ggtcctgcgg ggtggtggtg ccgcccagga
     1381 agctggcgct gcgcgcggtg tcgacgttga gatccgggtg agtcagatcg atcccgctgt
     1441 cgatgatcca ggcggtgcgg ccgccggtgt agttggcgcc gccgccgttg acgcgggcga
     1501 tgccccacgg ggtttcctgc ggcggctggg tgctgccacc gccgcccgga cgggcttcga
     1561 tgcggatcgg cggagcggcc atgacctggt cctgctcgca ataggcgatg ttcgggttcg
     1621 accgctgcat gttggccacc gcggcggcgg gcagatcggc cgcgaagcct tgcagcgcga
     1681 cgcggtagac gtgcttcact tggccgccgt gggccttcac cgagcggttg gcttcggatt
     1741 caacctgccc gcgcgaaacg ctcttgttga acacgcagat gtaggagttg gcgatcttgt
     1801 caccggcggc ttgcgcctgc gccggagaag ccagggcgac catcggcagc gcagcgagcg
     1861 caaggccaag catttttttg ttgagcatct tccctccgta gtggttgatc ggcgggaatt
     1921 gcgacgcgac ccgccaatgg aagcggagac taacgcggcc gcgaatcgtg tcgagagcaa
     1981 aaaatgctga attggggata ttgaccaatt tttgcagctt catcacgcag cggcggcgca
     2041 tgcagacgtt ttacttcgag catcgcccga aataggaaac aatatttcgt aaccggtgct
     2101 ttttactgct gcggcgccga cggcgattca ccattttcgt ccaccggcgg cggcggtgcc
     2161 tcgaaccggc ggtgccgcac gatatgcggc gtgatcacga tgtaaagctc ggtcttcgaa
     2221 ctggtcgacc gctcggtccg gaacagcgcc ccggcaatcg gaatgtcgcc cagcagcggc
     2281 accttggact tgcgcttgag gacgttttcc tgggtcagcc cgccgatcac gaaggtctcc
     2341 ccgtcgcgca cggtgaccga ggtttccgcc tcgcgctggc tgatcgtggg atagccctgg
     2401 ctgtagccgg tgaccgagga gaccacgcca tagatctgcg ctgtgacata gccatcgggc
     2461 gacacccgcg gagcgatctg cagggtcacg ccgacgttga cgtactgcac ctgctgcgac
     2521 accccgttga cgcccgagag cgtaatcgag gtgaggatcg gcagggcatc gccggtgatg
     2581 atcttggcgg tggagccgga ctgcgccgaa atccgcgggc gcgacaggat ccgcccttcg
     2641 cccttttcga tctgggcata gagcgcggcc tgcagcacgc ccgaaggcag gacatcgttg
     2701 ggatcgatgc tgaagggcag gaaagtgccg gtttcgatct tgccgatccc gatctgcccg
     2761 ttcgaattgt tgagatcgat ccccaggttc ttggccccgg tctcggtcag ctcgacgaac
     2821 tgggtttcca ggatcacgct gtccaccggc acgtcgatca gcgcgatctg ggccttgacc
     2881 tgggcgatcc gttccggcgt gcccgacacc cagatcgcgt tgagccggcg gtccaccccc
     2941 agcccgttgc cgaacgactg gcccagctgc ttggccgggg cctgctgctg ggtctgcatc
     3001 tgcagatact gcggctggtt gttcgatccg agcgagccaa agcccggttc gcggcggatg
     3061 aagacgttgt tcggctcgat cgtcgagcct tcgaccagga ggccgatcac ttcgctgaca
     3121 tcggcgtatt tcagcgggat cagctcaaag ctttcccccg ggcggaccgt catctgcgac
     3181 gaaagcgtgc tggccggggc cacggcggcg ccttcggagc cgaccgggcg gctgccggtg
     3241 gcctcttcct cgctgacctt gcggaccttg atctggagca cgttgggacc ggccggctcg
     3301 accgtgaccc gcgccgggct gctggtgtcg aaccgcagca ccagatcctg cccggccgtt
     3361 tcgaaccgcg cgctgcggac caggcccttg ccgctgaaca ccggcgtcac ctgcggcgca
     3421 cgcagcgcgt tgcggatgat gatcgcgggc ttcgtcggat tggtgttgag cggatcgatc
     3481 cgcggctcct tgggcgagaa gcgcagcatg taggcggcct gcccgtcgtc cgaggtgacc
     3541 ggcttcagtt cctccagaac ggccgggatc ggcgcgccct gcgcccgcag cggcgcttgc
     3601 ggcgccagca gcaggaccgg cgccgccacg gcaacggccg cggccatcag gcatttgcgg
     3661 aaactcagca agaacgaccc tcccggcctg cgctcgcgcg ggcctcaagc cggcgaacct
     3721 aacggcgcaa aagggattcg caaggggggc ggcagcgctg ccctgtcccg ccgccgcaca
     3781 ttgattgcgg ctgttgtgcc aagggcacag tgcgccccga tccccttaac catgccgttc
     3841 gtcgaacgcc ctctcgcttc cgccgtccgg gccgcctaag ctgctgcccg gactgtcaaa
     3901 ccgggggacc cgatcaacca tgcgccgcct tgccgccaaa ccgcttcttg cctcgctgct
     3961 tgccctgtcg ctcaccgcct gttcgactgg cccggcggcc gagctggcct cgaccccggc
     4021 tccggcatcc gccgcccccg cggccaagcc cgccccccag gctgcgccgc tgggcgaact
     4081 ggtcaaggcg gtggacattc cgtacgagaa gttcaccctg gcgaacgggc tgaccgtgct
     4141 ggtccacacc gatcgcaaag ccccgatcgt cggcgtcacg ctgtactacc gggtcggatc
     4201 gaagcacgag cccaagggcc gcaccggctt tgcccacctg ttcgaacacc tgatgttcgg
     4261 cggatcggaa aacgtcccca acttcgacgt gccgctggaa gcggccggct ccaccccgac
     4321 caacggatcg acctggtttg accgcaccaa ctatgtcgaa accgtgccca agggcgcggt
     4381 caacctggcg ctgttcatgg aaagcgaccg gatgggccat ctgctcggcg cggtgaccca
     4441 ggacaagctg gaaaagcagc gcggcgtggt gaagaacgaa aagcgccagg gggacaacga
     4501 gccctatggc ctggccgaat acgccattgg cgagggcctg ttcccggtcg gccaccccta
     4561 tcgccattcg accatcggct cgatggccga tctcgacgcc gccagcatgg aagacgtccg
     4621 caactggttc cgcggcaact atgcccccaa caacgtggtg ctggtgctgt cgggcgatat
     4681 cgacgccgcc acggcccggc cgatggtgga aaagtggttc ggccacatcc cgcgcgggcc
     4741 cgccgtggcg aaggtggagg ccggcccggt gaccctggcc gccccggtca agcgcgagat
     4801 gaccgaccag gtgccgctga cccgggtcta ccgggtgtgg agcggccccg ggctgaacga
     4861 tcccgatgcc ccggcgctgg acctgggcct caaggtgctg ggcgggctct cctcgtcgcg
     4921 gctcgacaac gcgctggtcc gcggcaaggg cgtggccgtt tcggtttcgg cctatgtcct
     4981 tcagtttgag cagaccagct atctgcaggt ccagatggac atcaagccgg gcgtggaccg
     5041 cgccgtggcc gaggccgcgc tggacgccga aatcgaaaag ctgatcaacg aaggcccgac
     5101 cgacgacgag ctgcgccgcg ccgcgaccac cgcggtgtcc ggccagatct ttgcgctgga
     5161 acaggtcggc ggcttttccg gcaagggcgc gcagctggcc gagggcgagc tctattcggg
     5221 cgatcccgcg cagttcaaga aggacctgga acggatcgcc gcgctgaccc cgctggacgt
     5281 caagggcgcg ctgcagcgct ggatgaagcg cccggtctat gccctggcga tcaccccggg
     5341 caagcgcacc gaaaagggcg agacgatggg cggctggggc gacgaggcga ccagcccgcc
     5401 gcccgccccc gacgccggca agcccgcgcc caagctggcc accggcccca agcgcgagtt
     5461 cccgccggtt gaggcggttg gcgatctcga tttcccgaag gtggaacgcg ccaccctgtc
     5521 gaacggcatc ccggtggtgc tggcccggcg cagcggcgtg ccgaccgtgc tggtcaacct
     5581 gaccttcgac gccggctttg ccgccgacgc gcgcgacaag cccggcacgc agaccctgct
     5641 gctctcggtg ctcgacgaag gaaccgagag ccgcgacgcg acccgcattg ccgaggacca
     5701 ggaacggctc ggcgcgcgga tcagcacggg gacggggatc gattcgtcca ccgtcaccct
     5761 ctcggcgctg tcggccaacc tggccccctc gctcgacctg atggccgacg tggtccgcaa
     5821 cccggccttc cgcgaggccg atgtggcccg cgcccgggat cagcagctgg ccgggattgc
     5881 ccaggccaat gccagcccct cggcgctggc caaccgctcg ctggggccga tcctgttcgg
     5941 cccggcccac ccttacggcc agcccagcga cgggctgggc gatgcccgct cggtcggcag
     6001 tctgactccg gcggcgctgc gcacggccca ggccgaatgg ttgcggcccg acaacctgac
     6061 catcaccgtg gtcggcgacg tttcgatgga ggcgctgctg ccgatgctgg aggcctcgtt
     6121 cggcacctgg caggccccgg ccacgccgcg cggcaccaag tcgcttgacg ccgcgatccc
     6181 cgcgccgcgc ccgcggatcg tcctgatcga ccggcccggt tcgccccaat cggtgatcgt
     6241 cgccgggcgg gtcctgccgc tgaccgggcg cgaccagggg ctggagccgc tggaactggc
     6301 caacgaggtg ctgggcaaca acttcctcag ccgcctcaac cttgatctgc gcgaggagaa
     6361 gggctggtcc tatggcgtgc gcagcgtggt gcgcagcccc agggggc
//
LOCUS       DASUDV010000540         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1852527,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000540 DASUDV010000000
VERSION     DASUDV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852527"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /gene="mrdA"
                     /locus_tag="VFV30_11880"
     CDS             <1..1438
                     /gene="mrdA"
                     /locus_tag="VFV30_11880"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX4848835.1"
                     /translation="GTPGARRVEVTATGKVVRDLGTREDVPGLPMKLTINAPLQDYAA
                     RRIGLESGAVVVMDCDNGDILAMASMPSYDPNRFSDGIGRIEWKMLSDDDHVPLRNKV
                     LRGLYPPGSTVKPAVAMSFLEAGLDPSATVSCSGGLRVGNRVFHCWERRGHGTVDMAK
                     AIYQSCDVYFYHFAQKIGMDHIASMARRLGLGQKFDLPVAGQSFGTVPDPAWKLKKYD
                     KPWAVYDTVNATIGQGYFLVNPLQQAVMAARIASGKALNPRFLFDPRQPEAPSLGFSQ
                     EHLDYVHAAMSEVVNGRGTAGRARLPIPEVLMAGKTGTAQVVGLNISKGKGGLWKHRD
                     HGHFIAFAPFDKPKYACAVVIEHGGGSSAAYPIARDVMTFLYDPAKAMQVLLEMETGW
                     GGTPQQRMEARYRSVAAKYGVNAPKAADDAEVERQGQSTDAAAAPPRTAATEAATPPP
                     EPVATTPSPAGPAAPAPGASAPAQEPTP"
     gene            1435..2550
                     /gene="rodA"
                     /locus_tag="VFV30_11885"
     CDS             1435..2550
                     /gene="rodA"
                     /locus_tag="VFV30_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX4848836.1"
                     /translation="MSRSIVPEAIAREPWQVLIPLFLLNTFGSLVLHSAAGGHLSPWA
                     LLHTIHFIVFLAMAFVISRLPRDFFTLTAYPIYGAIVVLLVMVDLIGGVRGGSQRWLD
                     LGFISLQPSELMKPAIILALARFLSSVPPATTQSWRILTPLGMLVGIPALLVMLQPDL
                     GTGLAICAGAAVVIFLSGLPMWWFVAAGGAVAAIAPVAFFTLLHDYQQKRVLTFLNPE
                     DDLLGSGYHITQSKIAIGSGGFFGKGFGNGSQSHLDYLPEAHTDFAFATMAEEFGVLG
                     GLFVLVVFLWTFRWGLNVARAAPDRFASLLAAGMTFTIFFYMAINLMMVMGLAPVVGI
                     PLPWISHGGSSLMTIMGCIGTIMAVERWSRRGGLQGV"
BASE COUNT          415 a          888 c          870 g          494 t
ORIGIN      
        1 cggaacgccg ggcgcgcggc gggtcgaggt taccgctacc ggcaaggtgg tgcgcgatct
       61 tggcacccgc gaggacgtgc cgggcctgcc gatgaagctg accatcaacg ccccgcttca
      121 ggattacgcc gcgcggcgga tcggcctgga aagtggcgcg gtggtggtga tggactgcga
      181 caacggcgat atcctagcca tggcatcgat gcccagttac gatcccaacc gcttttccga
      241 cgggatcggg cggatcgaat ggaagatgct gtccgacgac gatcacgtcc ccctgcgcaa
      301 caaggtgctg cgcgggctct atccgccggg ttcgacggtg aaaccggcgg tggcgatgtc
      361 gtttctcgag gccgggcttg atccttcggc cacggtcagc tgttcgggcg ggctgcgcgt
      421 cggcaaccgg gtgttccact gctgggaacg gcgcggccac ggcacggtgg atatggccaa
      481 ggcgatctac cagagctgcg acgtctattt ctatcacttc gcccagaaga tcgggatgga
      541 tcacatcgcc tcgatggcgc gccgcctggg gcttggccag aagttcgatc tgccggtggc
      601 cggccagtcg ttcggcacgg tgcccgatcc ggcctggaag ctgaagaaat acgacaagcc
      661 ctgggctgtc tacgatacgg tcaacgccac gatcggtcag ggctacttcc tggtcaaccc
      721 gctgcagcag gcggtcatgg ccgcgcggat cgcctcgggc aaggcgctca acccgcgctt
      781 cctgttcgac ccgcgccagc ctgaagcacc ctcgctgggg ttctcgcagg aacatctcga
      841 ttatgttcat gccgcgatga gcgaggtcgt gaacggacgc ggcaccgcgg ggcgcgcccg
      901 gctgccgatc cccgaagtgc tgatggccgg caagaccggc accgcgcagg tcgtcggcct
      961 caacatctcc aagggcaagg gtggcctctg gaagcaccgc gatcacggcc acttcatcgc
     1021 ctttgccccg ttcgacaagc cgaaatacgc ctgcgcggtg gtgatcgagc acggcggcgg
     1081 ttcttcggcc gcctatccga tcgcgcgcga cgtgatgacc ttcctctacg atccggccaa
     1141 ggcgatgcag gtcctgctcg aaatggagac cggctggggc ggcaccccgc aacagcgcat
     1201 ggaagcacgc tatcgttcgg tcgccgcgaa gtacggcgtc aatgccccca aggccgccga
     1261 tgatgccgag gtggaacgcc agggccagag caccgatgcc gccgccgccc caccacgcac
     1321 ggccgcaacc gaagcagcaa ccccgccgcc cgaacccgtt gccaccacgc caagccctgc
     1381 cggcccggct gcacccgcgc ccggcgcgtc cgcacccgcg caggagccga cgccatgagc
     1441 cgctcgatcg tgcccgaggc gattgcccgc gaaccctggc aggtgctgat tccgctgttc
     1501 ctgctcaaca ccttcggttc gctggtgctg cattcggcgg cgggcgggca cctctcccca
     1561 tgggcgctgc tccacaccat ccacttcatc gttttcctgg cgatggcctt cgtgatttcg
     1621 cgcctgccgc gcgatttctt cacgttgacg gcctatccga tctacggtgc cattgtcgtc
     1681 ctgctggtca tggtcgatct gatcggcggg gtgcgcggcg gcagccagcg ctggctcgac
     1741 ctgggcttta tcagcctgca gccgtccgaa ctgatgaagc cagcgatcat cctggcgctt
     1801 gccaggttcc tgtcgagcgt gccgccggcc accacgcaaa gctggcggat cctgaccccg
     1861 ctgggcatgc tggttgggat tccagccttg ctggtgatgc tgcagcccga tctgggcacc
     1921 ggcctggcga tttgcgcggg tgcggcggtg gtgatcttcc tgtcgggcct gccgatgtgg
     1981 tggttcgtcg cggcgggcgg cgcggttgcg gcgattgccc cggtcgcctt cttcaccctg
     2041 ctgcacgatt accagcaaaa gcgtgtgctg accttcctca accccgagga tgacctgctg
     2101 ggttcgggct atcacattac ccagtcgaag atcgcgatcg gatcgggcgg gttcttcggc
     2161 aagggcttcg gcaacggcag ccagagccac ctcgattacc tgcccgaggc ccacaccgac
     2221 ttcgcctttg ccaccatggc cgaggaattc ggcgtgctgg gcgggctgtt tgtgctggtg
     2281 gtgttcctgt ggaccttccg ttgggggctc aacgtggccc gcgctgcgcc tgatcgcttc
     2341 gccagcctgc ttgccgcggg catgaccttc acgatcttct tctacatggc gatcaacctg
     2401 atgatggtga tgggcctggc cccggtggtg ggcatccccc tgccctggat cagccacggc
     2461 ggctcatcgc tgatgacgat catgggctgc atcggcacga tcatggcggt cgaacgctgg
     2521 agccgaaggg gcggattgca gggcgtttga acgctcctgc aactttcccc ttttctccgg
     2581 cgaaactctc gctataggga gcgctccccg ccgaatcggc ggcccggcaa gccggtgtgt
     2641 ggacgcatag ctcagttggt agagcag
//
LOCUS       DASUDV010000541         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1653614,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000541 DASUDV010000000
VERSION     DASUDV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653614"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFV30_11890"
     CDS             complement(<1..578)
                     /locus_tag="VFV30_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4848837.1"
                     /translation="MATQALSPEQGFTYRRSPTATEAFEGWLKEHGQELPKHTEKWDV
                     TRSDIYVEDRWQPIFAEMRREAPVNKVHESPYGSYWNVTTIKTVQHVESLPELFSSSW
                     DRGGITIGDPPADIDPVKLAERRLPMFIAMDRPEHTGQRRTVAPAFTPGEIEKMAAEV
                     RMRTAATLDSLPFGEQFDWVDKVSIELTTGMLA"
     gene            complement(694..1929)
                     /locus_tag="VFV30_11895"
     CDS             complement(694..1929)
                     /locus_tag="VFV30_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4848838.1"
                     /translation="MSNGSPSGRLSVLAAALVVARRDFVAVLFSRSFFFFLLGPLFPV
                     IVGGLAGGIGASVRDQSDRAVVGVAMAEADAKLMVEAQQRLSRRLSNLPELVVVATPA
                     RGEPFNAMNALIDNRANAGAILSGTPEAPVLTGPKERTQQWRGPVSLIAAEALGKGAQ
                     TYPDVGTAAVATSRADERHGRILTAQAGQTLLFLLTMLLAGMVMSNLVEEKANKIIEV
                     LAAAIPMESLFLGKLFAMLGVSLVGIATWGLVGAGWALLAGNGVPLLATPAVGWLPFV
                     GLGVVYFSMAYLILGSMFLTIGGMATTVRDVQTLSMPVTMLQLLVFFFASYTMAQQGQ
                     PLELAAIVIPFSSPFAMLARAAQDERLWIHGVALAWQLFCVALFVRAGAYLFRSRVMK
                     SGPARADRKGWLRRKVKPA"
     gene            complement(1926..>2045)
                     /locus_tag="VFV30_11900"
     CDS             complement(1926..>2045)
                     /locus_tag="VFV30_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4848839.1"
                     /translation="EAGILSLSIERAGLHDAFVAIAGEAAARALEEDGEETVR"
BASE COUNT          366 a          688 c          657 g          334 t
ORIGIN      
        1 gccagcatgc cggtggtcag ttcgatcgaa accttgtcga cccagtcgaa ctgttcgccg
       61 aatggcagcg aatcgagcgt ggcggcggtg cgcatccgca cttcggcggc catcttctcg
      121 atctcgccgg gggtaaaggc cggggccacg gtgcggcgct ggccggtgtg ctcgggccgg
      181 tccatggcga tgaacatcgg caggcgccgc tcggccagct tgaccggatc gatgtctgcc
      241 ggcggatcgc cgatggtgat gccgccgcga tcccacgagg aggagaacag ttcgggcagc
      301 gattcgacat gttgcacggt cttgatcgtg gtgacattcc agtacgagcc ataggggctt
      361 tcgtgcacct tgttgaccgg cgcttcgcgg cgcatttcgg caaagatcgg ctgccagcgg
      421 tcctcgacat agatgtccga gcgggtaaca tcccactttt ccgtatgctt gggcaattcc
      481 tgcccgtgct ccttgagcca gccctcgaat gcttcggtgg cggtgggcga acgacgataa
      541 gtgaacccct gctcggggct gagtgcctgg gtggccatgg cttggctcct ctcgattgcc
      601 gcgttttccg gcaatgcgtt ttctgtcgag acttatcctg acggaactta ccttgttgtc
      661 aatagcggca ccgcgcttga ccgtatggat cgctcaggcc ggcttgacct tgcgccgcag
      721 ccagcccttg cgatcggcgc gtgccgggcc actcttcatc acccggctgc ggaacaggta
      781 ggcacccgcg cgcacgaaca gcgccacgca gaagagctgc caggccagcg ccaccccatg
      841 gatccacagc cgttcgtcct gcgcggcgcg ggccagcatg gcgaagggcg aggagaaagg
      901 tatgacgatc gccgccagtt cgagcggctg gccctgctgg gccatggtgt agctggcaaa
      961 gaagaacacc aggagctgca gcatggtcac cggcatcgac agggtctgca catcacgcac
     1021 ggtggtggcc atgccgccga tggtcagaaa catcgatccc aggatcaggt aggccatcga
     1081 gaagtagacc acgcccagcc cgacgaaggg cagccagccg accgccgggg tggccagcag
     1141 cggcacgcca ttccccgcca gcagcgccca gccggcgccg accaagcccc aggtggcgat
     1201 cccgaccagc gaaaccccca gcatcgcgaa cagcttgccc aggaacagcg attccatcgg
     1261 aatggccgcg gccagcacct cgatgatctt gttggccttc tcttcgacca ggttcgacat
     1321 gaccatgccc gccagcagca tggtcaaaag gaacagcagg gtctggccgg cctgtgcggt
     1381 cagaatcctg ccgtggcgct cgtccgcccg gctggtggcg accgccgcag tgccgacatc
     1441 gggataggtc tgggcaccct tgcccaacgc ctcggcggca atcagcgaaa ccggtccgcg
     1501 ccattgctgg gtgcgctcct tgggcccggt cagcaccggc gcttcggggg tgccagaaag
     1561 gatggctccg gcattggcgc ggttatcgat cagcgcgttc atcgcgttga acggctcacc
     1621 cctggccggg gtcgccacca ccaccagttc gggcaggttc gatagtcttc gcgagaggcg
     1681 ctgctgggcc tcgaccatca atttggcatc ggcttcggcc atggccacgc ccaccaccgc
     1741 gcggtcgctc tggtcgcgca cggatgcgcc gatcccgccg gcaaggccgc cgacgatcac
     1801 cggaaacagc ggaccgagca ggaagaagaa gaagctgcgc gaaaacagca cggccacgaa
     1861 atcgcgccgc gccaccacca gcgccgcggc cagcaccgaa agccggcccg aaggcgaacc
     1921 attgctcatc gcaccgtctc ctcgccgtct tcctcaagcg cgcgggctgc tgcctcgccg
     1981 gcaatcgcca cgaacgcgtc gtgcagcccg gcgcgctcga tcgagagcga gaggatcccc
     2041 gcttc
//
LOCUS       DASUDV010000542         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1454786,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000542 DASUDV010000000
VERSION     DASUDV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454786"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /gene="fliI"
                     /locus_tag="VFV30_11905"
     CDS             complement(<1..459)
                     /gene="fliI"
                     /locus_tag="VFV30_11905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012317.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEX4848840.1"
                     /translation="MLKLFDPLLDELGGLGIDLSPGRYGLVTACDGGLIEVSGLAAPV
                     GALCRLAHGHDKSLTAEVIGFRNGRTLMMLLGDTVMLRPGARVRPEGRPGMLSVGEAF
                     LGRAVDGEGQPIDGGAPIHTRSAWPAGGVRTGALDRSPVRLPFDTGVRALN"
     gene            complement(453..1019)
                     /locus_tag="VFV30_11910"
     CDS             complement(453..1019)
                     /locus_tag="VFV30_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX4848841.1"
                     /translation="MSSLPFGALAASSAFVCDPRFSDGAPPAVPIVTDPASDAWDQGY
                     AAGRSEAQAEAALAAQQGEADLARIELSLAHLDADLQEGLRHKLHATIVALCEVTLAP
                     LALDREALARRVAKASEMLARADDDKVLRLHPEDLALIADRLPPGLATEPDPGLERGA
                     LRIETAAGGVEDGPAHWRRAIGEALSQC"
     gene            complement(1012..>2016)
                     /gene="fliG"
                     /locus_tag="VFV30_11915"
     CDS             complement(1012..>2016)
                     /gene="fliG"
                     /locus_tag="VFV30_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX4848842.1"
                     /translation="AGLGDAERAAVMIMLLEEEQAAAILGELGPEELQLLGEKMCALG
                     DIGPEQITQAISGFVQNSGHSGISARGRTGHVRSLMNRAVGEVRAESLMQRILPMGEE
                     TGPAIELARWLTPQALIPLIKDEHPQAIAVLLVQLEPEVAAEVLHSLPAENQSAVVHR
                     VATLGPVSAEAIAMLEALLDQKIAQCHGKAALTMGGPREAANIINNAGKAAEKRIMPE
                     INKIDKALAREIEAEMFKFEHLFALSPQDMGALLREVESEVLIDALKGTVEEEREAFF
                     RAMSSRAADGVKDEIAARGRLKMAEVLEAQKAIVTIARRLAGEGTIQFGVGGGDDDYV
                     "
BASE COUNT          330 a          706 c          639 g          341 t
ORIGIN      
        1 gttgagcgcc ctgacgccgg tatcgaaggg tagccgcacc ggactgcgat cgagcgcgcc
       61 ggtgcgcacg ccgccagccg gccaggcact gcgcgtatgg atcggcgcac cgccatcgat
      121 cggctggcct tcaccatcga cagcgcggcc aaggaatgcc tcacccacgc tcagcatgcc
      181 gggccgtccc tcgggcctaa cccgcgcgcc cgggcgcagc attaccgtat cgcccagaag
      241 catcatcaag gtgcggccgt tgcgaaagcc gatcacctcg gcggtcagcg acttatcgtg
      301 cccgtgcgcc agccggcaca gcgccccgac cggcgcggcg aggccggaaa cctcgatcag
      361 accgccgtcg caagcagtga caaggccata gcggcccggc gaaagatcga tgccaagtcc
      421 gcccagttcg tccagaaggg gatcgaacag cttcagcatt gggacagcgc ctcgccaatt
      481 gcacgacgcc agtgcgcggg cccatcttcg accccgccgg ctgcggtttc gatccgcagc
      541 gccccgcgtt caaggccggg atcgggttcg gttgcaaggc cgggcgggag gcgatcggcg
      601 atgagggcga ggtcttcggg atggagccgc agcaccttgt catcatcggc ccgggccagc
      661 atctcgctgg ccttggcaac gcgccgcgca agggcttcgc gatcgagcgc aagcggggcc
      721 agggtcacct cgcacagcgc cactatcgtc gcgtggagct tgtggcgcag gccttcctga
      781 agatcggcat cgagatgcgc gagcgaaagc tcgatccggg ccagatccgc ttcgccttgc
      841 tgcgctgcca aagcggcctc ggcctgcgct tcggaacgcc ccgcggcata gccctggtcc
      901 caggcatcgg aagcgggatc ggtgacaatc ggcaccgctg gcggcgcgcc atccgaaaag
      961 cgcggatcgc agacgaaggc gctcgatgcg gccagcgccc cgaacggcag gctagacata
     1021 atcgtcgtcc ccgccgccaa ccccgaactg gatcgtgcct tcgccggcca gacgccgggc
     1081 aatcgtgacg atcgccttct gcgcttcgag cacttcggcc atcttgagcc gcccgcgcgc
     1141 ggcgatctcg tccttgacgc catcggcggc gcggctcgac atggcgcgga agaaggcttc
     1201 gcgctcttcc tcgaccgtgc ccttcagcgc atcgatcagc acctcgcttt ccacctcgcg
     1261 cagcagtgcg cccatatcct gcgggctgag cgcgaagaga tgctcgaact tgaacatttc
     1321 tgcctcgatc tcgcgcgcga gtgccttatc gatcttgttg atctcgggca tgatccgctt
     1381 ttcggcggcc ttgccggcat tgttgatgat gttcgcggcc tcgcgcgggc cgcccatggt
     1441 tagggcggcc ttgccgtgac actgggcgat cttctggtcg agcagcgcct cgagcatggc
     1501 aatcgcctcg gcggaaaccg ggccaagcgt tgccacccgg tggaccaccg cgctctggtt
     1561 ttcggctggc aacgaatgca gcacttcggc ggcgacttcg ggctcaagct ggaccagcag
     1621 caccgcgatc gcctggggat gttcatcctt gatcagcggg ataagtgcct gcggggtcag
     1681 ccatcgtgcc agttcgatcg ccgggccggt ttcctcgccc atgggcagga tccgctgcat
     1741 caggctttct gctcttacct cgcccaccgc gcggttcatc agcgagcgga catggcccgt
     1801 acgaccgcgc gcgctgatgc cgctgtgtcc ggagttctgc acgaaaccgg aaatcgcctg
     1861 ggtgatctgt tcagggccaa tatcacccag cgcgcacatc ttttcgccca gcagctggag
     1921 ctcttcgggc cccagttcgc caaggattgc cgctgcctgc tcttcctcca gcaacatgat
     1981 catcaccgcc gcgcgctcgg catcgccaag gccggc
//
LOCUS       DASUDV010000543         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1874613,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000543 DASUDV010000000
VERSION     DASUDV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874613"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFV30_11920"
     CDS             complement(<1..480)
                     /locus_tag="VFV30_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4848843.1"
                     /translation="MKGIVLAAGRGSRIGDATRTLPKPLLEVGSRRCIDFAIDALLAR
                     VDEVIVVTGYMAEAVEAYLDTRGDADRVRSVRNSALEAGNLTSLRAARALIGDEPFVV
                     TNADHLFPSDMYTRHFIPAEGLTIACERGRPILDDEMKVVEREGRLAAMAKTLTEFDG
                     "
     gene            complement(560..1657)
                     /locus_tag="VFV30_11925"
     CDS             complement(560..1657)
                     /locus_tag="VFV30_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4848844.1"
                     /translation="MTLPTIVIPATPFLRQTMGGLSGIARHVIVAELAGARRIVIVAD
                     SPIEPDWEKSFALRERALPEIEVIADPGSAAAGPKLLMPGDRLLSAAGMQRLLAGVPI
                     EELQEGAESFSAGEPREVTWLLLLATLKAGEGWVGRNLNRPISFRMAALAMRIGITPN
                     QITWLTFLIAVVMAGLLGLGGAVGLAIGGALYQVVSVVDCVDGDIARVSYLSSRWGAA
                     LDTALDMIANLGFMGGLGVGLVRTYGSEQLLAAGLLVAVAATAMAFMTILLRIGPKRG
                     SFDVLRAALEVRLAATPRLRAVVMTAEKMFKRDFYALFFATLCLLGLAKLLLWLALGG
                     AIIWVLAIAWCAHLIAGDKQGALLPGHLRGG"
     gene            1863..2732
                     /locus_tag="VFV30_11930"
     CDS             1863..2732
                     /locus_tag="VFV30_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX4848845.1"
                     /translation="MRPLRPRELQDPLNWYFYHPLAWRIALVLRHTPITPNMVSVLGG
                     TMVVLAAIAYSTLSWPVSAALGMALHLSWHVIDGADGDLARITGRTSPVGEMVDGLCD
                     YLSHVVLYLVLGWVLAHSTGPVNGWQAWVLIVLAGIGHAVQANHVEVQRRQYQWWVYG
                     TAWIRNSHGSSDSATSRSWASMLVTAYLDVASGMTPHALRIDAAVEAAKGNRQRLDQI
                     AAAVRKEAPPLLLVCKVLGPNPRAVVLGLSMFAGSPLWYMLYQAVVLNLLLVVSVRMH
                     NAAARRIAAAIGV"
     gene            2737..3954
                     /locus_tag="VFV30_11935"
     CDS             2737..3954
                     /locus_tag="VFV30_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4848846.1"
                     /translation="MLNPDLLDEARAISDEIVALRRAIHAEPELGLHTPLTRDKVRAA
                     LANLPLEWVEGPSTTGLVATLKGGAGEGRCVLLRGDMDALAMPEETGLDSASRIEGTM
                     HACGHDAHTAMLAGAVRLLCARRDELKGEVRFMFQPGEEGFHGARHMLEDGLLGGEGT
                     GRTLPEAAFALHVMPNAPHGLVTGRVGALLASADQFDVSVDGRGAHAAMPHHGLDPVP
                     IACEIVMALQALVARQFDAHDPVIATVARIDAGTAHNIIADFAHLNGTLRTLSPRHRA
                     KLHAGLEQLATGIAAAHGASARVTITPGFPVTNCDARAVALGETVARELFGEAAFRRL
                     DQPIMGAEDFSYVLEQVPGAMFFLGVAKEGADWHACPAIHSPRMTVDEAALPKGAALL
                     AGCATRFLERGWD"
BASE COUNT          718 a         1392 c         1409 g          693 t
ORIGIN      
        1 accgtcgaat tcggtcagcg tcttggccat ggcggcaagg cgcccttcgc gttcgaccac
       61 cttcatctcg tcatccagga ttggacggcc acgctcgcag gcaatggtca acccttcggc
      121 ggggatgaaa tggcgggtat acatgtcgga aggaaaaagg tgatcggcat tggtcacaac
      181 gaagggctcg tcgccgatca gggcccgcgc ggcgcgcagc gaggtcaggt tgccggcttc
      241 cagtgcgctg ttgcggaccg accgcacacg gtctgcgtcg ccgcgtgtat cgagataggc
      301 ctcgaccgcc tcagccatgt agccggtgac aacgatcacc tcatcaaccc gggccagcag
      361 cgcgtcgatc gcgaaatcga tgcagcggcg cgaaccgact tcgagcagcg gcttgggcaa
      421 ggtcctggtg gcatcgccaa tccggctgcc acgccctgct gcgagtacaa tgcccttcat
      481 cgctatccgg gaaagtttga accaagggga atgacgcgcc cttgcagccc gccttgcccc
      541 tgtcaaggcg cggggtcggt cagccgcccc tgaggtggcc gggaagcagc gcgccttgtt
      601 tgtcgcccgc gatcaggtga gcgcaccagg cgatcgccag aacccagatg atcgcgccgc
      661 ccagggccag ccacagcagc agcttggcca ggccaagcag gcacagcgtg gcaaagaaca
      721 aggcgtagaa atcgcgcttg aacatttttt ccgcggtcat caccacggct cgaaggcgcg
      781 gcgttgccgc caggcgcact tcgagcgcgg cgcgcagcac atcgaaactc ccgcgtttcg
      841 gcccgatccg caacaggatc gtcatgaacg ccatggccgt ggcggccacg gcaaccagca
      901 gaccggcagc cagcagctgc tcgctgccat aggtccgcac cagacccaca cccagcccgc
      961 ccatgaagcc aaggttggcg atcatgtcga gcgcggtgtc gagcgccgca ccccagcggc
     1021 tggaaaggta gctgacccgg gcaatatcgc catcgacgca atcgaccacc gaaacgacct
     1081 ggtagagcgc gccgccaatc gccagtccga ccgcgccgcc cagccccagc agcccggcca
     1141 taaccaccgc gatcaggaaa gtgagccaag tgatctgatt tggggttatc ccgatccgca
     1201 tcgccaacgc cgccattctg aacgagatcg gccggttgag attgcgcccg acccagcctt
     1261 cgccggcctt gagcgttgcc agcagcagca gccaggtcac ttcgcgcggt tcaccggcgg
     1321 aaaaggactc ggccccttcc tgaagctctt cgatcggtac gccggcaagc agccgctgca
     1381 ttcctgctgc gctcagcagc ctatccccgg gcataagcag tttcggcccg gcagctgcgc
     1441 tgcccggatc ggcgataact tcaatctcgg gtagtgcccg ctcgcgcagg gcaaagcttt
     1501 tttcccagtc cggctcgatc gggctgtcgg caacgatcac gatgcgccgg gcgccggcca
     1561 gctcggccac gatcacgtgc cgggcgatcc cgctcagccc gcccatggtc tggcgcagga
     1621 acggcgtggc aggaatgacg atcgtgggca atgtcacggg ttcagggaca catcaggcag
     1681 aggaaggatg gcttggaaag ggcgaaattt cgggccgctg cttaggtctg gggtgaaatg
     1741 ttgtccaatc ctttgttgcc catcggagcc gcctcggata ggccttggga cttcgggaaa
     1801 cacaatacga caggtcaggg ccgggacgag cggtggtggc gatcaacacg aatgaacggc
     1861 cggtgcgccc cctgcgcccg cgcgagctgc aggatccgct caactggtat ttctaccatc
     1921 cgctggcctg gcggatcgcg ttggtattgc ggcacacgcc gatcaccccg aacatggttt
     1981 cagtgctggg cggaaccatg gtggtgctcg ccgcgatcgc ctactcgacg ctgagctggc
     2041 cggtttcggc ggcactgggg atggcgctcc atctgtcctg gcatgtgatc gacggtgccg
     2101 atggcgatct ggcccggatc accgggcgga ccagtccggt gggcgaaatg gtcgatgggc
     2161 tgtgcgatta tctcagccac gtggtgctct atctcgtgct cggctgggtg ctggcgcact
     2221 cgaccgggcc ggtgaacggc tggcaggcct gggtgctgat cgtgcttgcc gggatcggcc
     2281 acgcggttca ggccaaccat gtcgaagtgc agcgccgcca gtaccagtgg tgggtctatg
     2341 gcacggcctg gatccgcaat tcccatggga gcagcgattc ggccacctcg cgcagctggg
     2401 cgagcatgct ggtcaccgcc tacctcgatg tcgccagcgg gatgaccccg cacgcgctca
     2461 ggatcgacgc cgcggtcgag gctgccaagg gtaaccgcca gcggctcgac cagatcgccg
     2521 cagcggtgcg caaggaagcc ccgccgctgc tgctggtctg caaggtgctg ggccccaatc
     2581 cgcgcgcggt ggtgctgggc ctgtcgatgt tcgcgggcag cccgctgtgg tacatgctct
     2641 accaggcggt ggtgctgaac ctgctgctgg tggtctcggt caggatgcac aacgccgcgg
     2701 cccggcggat cgccgccgca atcggagtgt gacgcgatgt tgaacccgga cctgctggac
     2761 gaagcccgcg cgatctcgga cgaaatcgtg gccttgcgcc gcgcgatcca tgccgaaccg
     2821 gaactggggc tgcatactcc gctaacccgc gacaaggtac gcgccgcgct ggccaacctg
     2881 ccgctcgaat gggttgaagg gccttcgact accgggctgg tcgcaacgct gaagggtggg
     2941 gcgggcgaag ggcgctgcgt gctgctgcgc ggcgatatgg atgccctcgc catgcccgag
     3001 gaaaccgggc tcgactctgc ctccaggatc gaaggcacga tgcacgcctg cggccacgat
     3061 gcgcacaccg cgatgctggc cggggcggtg cggctgctgt gcgcgcggcg cgacgaactc
     3121 aagggcgaag tgcgcttcat gttccagccg ggcgaggaag gcttccacgg cgcccgccat
     3181 atgctcgagg atggcctgct ggggggcgag ggcaccggcc gcaccctgcc cgaagcggcc
     3241 tttgcgctgc atgtcatgcc caacgcgccg cacggcctgg tgaccggacg ggtcggcgcg
     3301 ctgctcgcct cggccgatca gttcgatgtg tccgtcgacg ggcgcggcgc ccatgcggcg
     3361 atgccgcacc atgggctcga tccggtgccc atcgcctgcg agatcgtcat ggcgctgcag
     3421 gcgctggtcg cgcgccaatt cgatgcccac gatccggtga tcgccaccgt ggcgcggatc
     3481 gacgccggca ccgcgcacaa catcatcgcc gatttcgcgc acctcaacgg caccttgcgc
     3541 acgctttcgc cgcgccatcg cgccaagctg cacgccgggc tcgaacaact cgccaccggg
     3601 atcgcagccg cgcacggcgc cagcgcgcgg gtgacgatca cgccgggctt tcccgtaacc
     3661 aattgcgatg cccgcgcggt tgccctgggc gagacggtag cgcgcgagct gttcggcgag
     3721 gcggcattcc gccggctcga ccagccaatc atgggcgccg aggatttctc ctatgtcctc
     3781 gagcaggtgc cgggagcgat gttcttcctc ggcgtcgcca aggagggagc cgattggcat
     3841 gcctgtccgg caatccactc gccgcgcatg accgtcgacg aggcggccct gcccaaaggc
     3901 gcggcgctcc tcgccggctg cgcaacacgg tttctggaac ggggctggga ttagctggcg
     3961 gcggcgatgc agcttaccgc tgcaatccgg ggattgtccg ccgggcggct ggcgatgggg
     4021 cctgcgaaca gctggcaggg aggatcgccg caatctgatc gaggatttcg gcagcgtttg
     4081 cgctattgat gctgtgccca ttggtggtca ggccgcgcgc cggaagcccg gttgacgcca
     4141 gttgcggcaa gaactcgccc gccgggccca cttcgatgtc gttttcgcca atgaacaaaa
     4201 gggttttggc ca
//
LOCUS       DASUDV010000544         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_261631,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000544 DASUDV010000000
VERSION     DASUDV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261631"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..861)
                     /locus_tag="VFV30_11940"
     CDS             complement(88..861)
                     /locus_tag="VFV30_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX4848847.1"
                     /translation="MATQLSTHSRNLDCSEAEWQVRQQLAACYRIFDHLGWSESIYNH
                     ISVKVPDEEGAFLINPFGLLYSEVCASNLVKIDIDGNTLDGSTYPVNKAGFVQHSYFH
                     RHVPWAHAICHTHTTATMAVCSLEGGLQPVNFYACNFAGRLAYHDFEGVTVRSEEGER
                     LLANLGDKRILMLKNHGPVILGRSLPEIFIMHWSLQRACEVQLATMSMGKPLTVPDEV
                     VAVHQRDLHESQVPGTQPGAADFEAWVRRIDKVDRSWRD"
     gene            complement(904..2274)
                     /locus_tag="VFV30_11945"
     CDS             complement(904..2274)
                     /locus_tag="VFV30_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848848.1"
                     /translation="MAASVRARFESRSATLRGVVPLASDIELQARALLFDDRRTLRFS
                     GADSSASGQDASLRLVGKGAWQFDVLGYVQARDFTNVVISSTSFRKTLDQRATPSTGL
                     GTKIELRPPLGDEHPLRLGADWRRASGTMFETAFSAASGAITARRSAGGRNDDLGFFA
                     EGDRSFGPLRLTASVRADRWSINEGRFTEINGAGLVTANRIYPDHAGWAGSLRGGAAL
                     DLGGGLALRGAGYSGLRQPTLNELYRPFVVFPVTTRANAALANERLRGFEAGIDLRPG
                     KGIELALTAFDNRIKGAIANVTIGTNLRERQNLDAVHARGLEAQVRASHGPFEWDGSL
                     SLVDAKVEASGAALPLDGKRPAQVPEFSAATTLRWRPAQGWLLAASLRHVSSQFEDDL
                     GIDSLPAATTLDAWASVPLGQRLSLILRGENLTDARVITRNQAGSIDLGAPRTFWAGL
                     RISMGR"
     gene            complement(2317..>2721)
                     /locus_tag="VFV30_11950"
     CDS             complement(2317..>2721)
                     /locus_tag="VFV30_11950"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX4848849.1"
                     /translation="QLSGIAGFQQFRRSDSRSSNPSAQGVTLRGLGGNASSRTLVLLD
                     AVPVTDPFFGFVPFSAMPPERLGRITLIPGASSNAFGAGGVAGTLDLSSASPGELPLV
                     EGAVLGNDRGESELSATFAPRLGQGYLVASGR"
BASE COUNT          483 a          910 c          879 g          449 t
ORIGIN      
        1 cgcagagccc acaacagcgg cgtttcggga tcgagccgca gttcgaccgc gcggccgttg
       61 acggtaagct tgctcacgcg ccaacgctca atcgcgccat gaacggtcaa ccttgtcaat
      121 ccgccgcacc caggcctcaa aatcggcggc gcccggctgg gtgccgggaa cctgcgattc
      181 gtgcagatcg cgctggtgga ccgcgacgac ctcgtccgga acggtcaggg gcttgcccat
      241 cgacatggtc gccagctgca cctcgcaggc gcgctgcaac gaccagtgca tgatgaagat
      301 ttcgggcagc gaccgaccca ggatgaccgg cccgtgattc ttcagcatca ggatccgctt
      361 gtcacccagg ttggcgagca gccgttcgcc ttcttcggag cggacggtca ccccttcgaa
      421 atcgtggtag gccagccgtc cggcaaagtt gcaggcatag aaattgaccg gctgcagccc
      481 gccttccagg ctgcagaccg ccatggtcgc ggtggtgtgg gtgtggcaga tggcatgagc
      541 ccagggcacg tggcgatgga agtacgaatg ctggacgaag ccggccttgt tgaccggata
      601 ggtgctgcca tcgagcgtgt tgccgtcgat gtcgatcttg accagatttg aggcgcagac
      661 ctcggaataa agcaggccga acgggttgat caggaaggcg ccttcctcat cgggaacctt
      721 gaccgagatg tggttgtaga tcgattccga ccagccaaga tggtcgaaga tccggtagca
      781 ggcggcgagc tgctggcgca cctgccattc ggcctcgctg caatcgagat tgcggctgtg
      841 ggtgctgagt tgggttgcca tcggtctctc cttttcgcct cttcgcgcca aaccctaagc
      901 gatctaacgc cccatggaaa tgcgcaatcc ggcccagaat gtgcgcggcg cgccaagatc
      961 gatcgatccg gcctggttgc gcgtgatcac ccgcgcatcg gtgaggtttt cgccgcgcaa
     1021 gatcaggctg agccgctggc ccaacggcac gctggcccag gcatcgagcg tcgttgcggc
     1081 tggcaggctg tcgatcccca gatcatcctc gaactggctt gagacatggc gcaggctcgc
     1141 cgcaagcagc cagccttgcg ccgggcgcca gcgcagcgtg gtggcagcgg aaaattcggg
     1201 gacctgcgcc gggcgcttgc catcgagcgg caaggccgcc ccgctcgcct cgaccttggc
     1261 atcgaccagg ctgagcgaac cgtcccattc gaacggtcca tggcttgccc gcacctgcgc
     1321 ctcgagaccc cgggcatgga ccgcatcgag attttgccgt tcgcgcaggt tggtgccgat
     1381 cgtgacattg gcgatcgcgc ccttgatgcg gttgtcgaag gcggtcagcg ccagctcgat
     1441 ccccttgccg gggcgcagat cgatgccagc ctcgaacccg cgcagccgct cgttggcaag
     1501 cgcggcattg gcccgggtgg taaccggaaa gaccacgaag ggccggtaaa gctcgttgag
     1561 ggtcggctgg cgcagaccgc tatagcccgc cccgcgcagc gccagccccc cgcccagatc
     1621 cagcgccgcc ccgccgcgca ggctgcctgc ccagccagca tgatcggggt aaatgcggtt
     1681 cgccgtaacc agccctgccc cgttgatttc ggtgaaccgg ccctcgttga tcgaccagcg
     1741 atcggcccga accgaagctg tcagccgcag cgggccaaag cttcggtcac cctcggcgaa
     1801 gaagcccaga tcgtcgtttc ttccgcctgc gctgcgccgc gcggtgatgg ccccgctcgc
     1861 cgcgctgaag gcggtttcga acatggtgcc gctggcccgg cgccagtcgg cacccagtcg
     1921 caagggatgt tcatcgccca gcggcgggcg cagttcgatc ttcgtaccaa gcccggtcga
     1981 aggggtggcg cgttgatcca gggtcttcct gaagctggtc gagctgatca cgacattggt
     2041 gaaatcgcgc gcctggacat agcccagcac gtcgaattgc caggcaccct tgccaaccag
     2101 acgcagcgat gcgtcctgcc cgctggcgct gctgtcggcg ccggaaaaac gcaaggtccg
     2161 gcgatcgtcg aacagcaggg cgcgggcctg cagttcgata tccgaagcca gcggcaccac
     2221 accgcgcagc gtggccgaac ggctttcaaa ccgcgccctg accgaagcgg ccacgcgctg
     2281 cgtcgcgggc gtggtccaaa aaccctgacc cttgtcctag cgtccgctag ccaccagata
     2341 gccctgacca agacgcggcg cgaaggttgc agaaagttcg ctttcgccgc gatcgtttcc
     2401 cagcacggcg ccttcgacca gtggcaattc acccgggctg gcgctggaca gatcgagcgt
     2461 gccggccacc ccgcccgcgc cgaatgcatt gctgctcgct ccggggatca gggtgatccg
     2521 gcccagtcgc tcgggcggca tggcgctgaa cggcacgaag ccaaagaagg gatcggtcac
     2581 cggcaccgca tccagcagca ccagggtgcg gctcgaggca ttgccgccaa gcccccgcag
     2641 cgtcacccct tgcgccgaag ggttcgacga gcggctatcg gaacggcgaa actgctggaa
     2701 gccggcgatc ccgctcagct g
//
LOCUS       DASUDV010000545         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_770180,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000545 DASUDV010000000
VERSION     DASUDV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770180"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFV30_11955"
     CDS             <1..600
                     /locus_tag="VFV30_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX4848850.1"
                     /translation="AATLTCAGLTAWRALFVDNAVKPGDTVLVQGTGGVSVFALQMAK
                     AAGATVIATSSSDAKLERMQALGADHLINYKAEPAWGMKAMMLTGGAGVDCVVEVGGA
                     GTLDQSMIATRVGGHVALIGVLAGFAGPVQTALLMAKNLRVQGLTVGSRQQQLDMIAG
                     IEANGIKPVISDHFPLERLADAFRHQAANAHFGKIAVDI"
     gene            complement(636..1712)
                     /locus_tag="VFV30_11960"
     CDS             complement(636..1712)
                     /locus_tag="VFV30_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HEX4848851.1"
                     /translation="MPQTVQFARGNLARHRIADSASAPLGEGAVRLRIESFSVTANNV
                     TYAVVGDAFGYWNFFPGEGEWGVVPMWGHAVVEASNHPDIAVGERVYGYLPMGTHLDV
                     EPVGVSAGGFTDGAAHRQPMSPIYNQYSRLAADPEHDPAKEAERMIFGPLFKTGFLIE
                     SFLRREDWFGAQAVVITSASSKTALSLASVARHRSPGVKRIGLTSSGNVDFVKASGFY
                     DEVLAYDRIADLPQVPSVSVDFAGNAGVLHAIHTALGDHLKYSCTVGATHVGEGFGRD
                     NGPMPGPTPTLFFAPDHAVAAIKELGPKAFGEAVAESWVRFLGEAGGAVAVSERGGLT
                     AAAAAFAAIHSGQADPAVGIVIRP"
     gene            complement(1823..2170)
                     /locus_tag="VFV30_11965"
     CDS             complement(1823..2170)
                     /locus_tag="VFV30_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848852.1"
                     /translation="MKAILPFALAALAFAAPAAAQTANDDLICVAWAANSGDQASSEE
                     EKDVMAMAMFYYIGRWEAATGRPLEAGLTPKFVADNYKAISTAGPTCQGRMLELAKRM
                     QVAGQALINSSKR"
BASE COUNT          367 a          745 c          803 g          348 t
ORIGIN      
        1 gccgccacgc tgacctgcgc gggcctgacc gcctggcgcg cgctgttcgt cgacaacgcg
       61 gtgaagcccg gcgatacggt gctggtccag ggcaccggcg gggtttcggt cttcgcgctg
      121 cagatggcca aggcggccgg ggccacggtg atcgccacct cctcgtccga tgccaagctg
      181 gaacggatgc aggcgctggg ggcggaccat ctgatcaatt acaaggccga gccggcctgg
      241 ggcatgaagg caatgatgct gaccggcggc gcgggcgtgg actgcgtggt cgaagtgggc
      301 ggcgcgggaa cgctggacca gtcgatgatc gccacccggg tgggcggcca tgtcgcgctg
      361 atcggggtgc tggcgggctt tgccgggccg gtccagaccg cgctgctgat ggccaagaac
      421 ctgcgcgtcc aggggttgac ggtgggcagc cgccagcagc agctggacat gatcgccggg
      481 atcgaggcga acgggatcaa gccggtgatt tccgatcact tcccgctgga gcggctggcc
      541 gacgccttcc gccaccaggc cgccaacgcg cacttcggca agatcgcggt ggacatctga
      601 ggggcgtggt cccgggcctg acccgggacg gcgttttacg gccggatcac gatccccacc
      661 gccggatcgg cctggccgga atggatcgcg gcgaaggccg cggccgctgc ggtcaggccg
      721 ccgcgctcgg acaccgcaac cgcgccgccg gcctcgccaa ggaagcgcac ccagctttcc
      781 gccacggcct cgccaaaggc cttggggccc agttccttga tcgccgccac agcgtgatcg
      841 ggggcgaaga acagcgtcgg cgtggggccc ggcatcggtc cgttgtcccg cccgaagcct
      901 tcgccgacat gggtggcgcc gaccgtgcag gaatacttga ggtgatcgcc cagcgcggtg
      961 tggatggcgt ggagcacgcc cgcgttgccg gcaaaatcga ccgagaccga gggcacctgc
     1021 ggcagatcgg caatgcggtc ataggccagg acctcgtcat agaacccgct ggccttcacg
     1081 aagtcgacat tgcccgaaga ggtcagcccg atccgcttca ccccgggcga gcggtggcgc
     1141 gcgacgctgg cgagcgacag cgcggtcttc gacgaggccg aggtgatcac cacggcctgc
     1201 gccccgaacc agtcttcgcg gcgcaggaag ctttcgatca ggaacccggt cttgaacaga
     1261 gggccgaaga tcatccgctc cgcttccttg gccggatcgt gttccggatc ggcggcgagg
     1321 cggctgtact ggttgtagat cgggctcatc ggctggcggt gcgccgcgcc gtcggtaaag
     1381 ccgccggcgc tgacgccgac cggctccacg tccaggtgcg tgcccatcgg cagatagccg
     1441 taaacccgtt cgcccactgc gatgtccggg tggttcgaag cctcgaccac ggcgtggccc
     1501 cacatcggca cgacgcccca ttcgccctca ccggggaaga agttccaata gccaaaagca
     1561 tcgccaacca ccgcgtaggt gacgttgttg gcggtgaccg agaagctctc gatccgcagc
     1621 cgcacggcgc cttcgcccag cggtgcagag gcgctgtcgg cgatccggtg gcgggcaaga
     1681 ttgccccggg cgaactgaac ggtttgcggc atggcttgtc ctctcacgca gcgggcccga
     1741 tgcccgccgc gctgtgcaat caagcaggag aagcccggcc ggtaaagcgg caatccgccc
     1801 cccggccgcg ggattcaggc gcttagcgct tggacgagtt gatcagggcc tggccggcca
     1861 cctgcatccg cttggccagc tcgagcatgc gcccctggca ggtcggaccg gcggtcgaga
     1921 tcgccttgta gttgtcggcc acgaacttcg gagtcagccc ggcttcgagc ggacggccgg
     1981 tcgcggcttc ccagcggccg atgtagtaga acatggccat cgccatgacg tccttttcct
     2041 cttcgctgga ggcctgatcg cccgaattgg cggcccaggc gacgcagatc aggtcgtcgt
     2101 tggcggtctg cgcggcggca ggagcggcaa aggccagcgc ggccagagcg aagggaagaa
     2161 tggctttcat ggatcagacc ctcatcggca tgagcacgta cagtgcagga cttttctcat
     2221 cctggcggat cagggtcggc gcgccggcgt cggcgaggtg gag
//
LOCUS       DASUDV010000546        10312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_505426,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000546 DASUDV010000000
VERSION     DASUDV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505426"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFV30_11970"
     CDS             <1..720
                     /locus_tag="VFV30_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX4848853.1"
                     /translation="KALVAAERLDFGIAFDGDGDRIGAIDGQGRVIWGDQLLMIYAED
                     LLKMEPGAAIIADVKASRALFERVSQLGGRPTMWKTGHSLIKSKMKEIGAPLAGEMSG
                     HVFFAHQYYGFDDALYAAIRLIAASARLGKSVTELRGAMPELVNTPELRFQVDESRKF
                     AVIAEVKERLSAAGADVDATDGVRVSTDDGWWLLRASNTQDVLVARAESESEAGLERL
                     MAQIDGQLAASGLERGPQAGH"
     gene            complement(750..2015)
                     /locus_tag="VFV30_11975"
     CDS             complement(750..2015)
                     /locus_tag="VFV30_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEX4848854.1"
                     /translation="MHPIDYSEPTAIGLALALGLLIGVQRGWASRTGAPGSRFAGIRT
                     HALFGLAGGIAGALQERASGLATVLLAAAAALVVLGYWRSTKGRASISGTASLTGLIT
                     LACGFLAGSGDFTLASALAGTTVLVLSQRSRWHHWLTHIDEHEMLAIARFALIALVIL
                     PLLPDTPMGPYGAWRPRQLWLVVVLVSGFSFAGYAAVRLLGPARGVLATAAAGSMVSS
                     TAVTASLAGKLRDGAGDAMVLNAGVALASATMFLRVMLLTAALAWFALPGFATLAAPG
                     MVVSLAACWLILRRRGEGTGQLEDGVKLRNPFDLGPAVLLMLLTMALTLAARWVLERY
                     GDAGLATVLAISGTVDVDSAIITMGGLPPGTLTARVAALVLALPVTLNTLFKTATLLS
                     IAGWRKAWPGASAMLASLAALGGGVLVVW"
     gene            2093..4546
                     /locus_tag="VFV30_11980"
     CDS             2093..4546
                     /locus_tag="VFV30_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX4848855.1"
                     /translation="MPDLLPPELSGWFAAKGWRLRRHQAEMLEVAAAGRHALLVADTG
                     AGKTLGGFLPTLADFCPSRLAGHAPPEGLHTLYVSPLKALAHDVQRNLLTPVADLDLA
                     MRVEVRSGDTPSERKARQRSNPPHVLLTTPESLSLLLSYPEAEHLFAGLQRVVIDEIH
                     AFAPTKRGDLLALALTRLQALAPGLQRVGLSATLADPADFAGWLAPGGAAETVSLVTG
                     EQGAAPELAILLPDEARVPWGGHAAAWAVPQLYEAIRANRTTLVFCNTRFLAEFIFQQ
                     LWNANADVLPIGIHHGSLSKEARRKVEGAMARGELRALVATSSLDLGVDWGDIDLVVQ
                     MGAPKGSSRLLQRIGRANHRLDQASRALLVPGNRFEFLEAFAAEQAVEEGQRDGEAFR
                     PGGLDVLAQHVMARACAGPFDGEALHREVCSARPYAWVDAGIWERVLHFVATGGYALK
                     AYDRFQRIVRGRDGLWRLTHPDHAARFRLNAGIIIDSEMIEVRFRNGRSLGRVEESFA
                     AALRPGDTIRFAGLDLEIEAMREAELVVRAAKKAGQIPSYMGQRLPLTTHLAERVQTL
                     LADRAGWARFPDDVREWLEVQDWRSELPGPGRLLVESFPHESKHYTVFYCFEGWPAHQ
                     SLGMLITRRMEERGLAPMGFVANDYALAVWSLRPVDDPATLLSPDILADEFVAWVEDS
                     YLLRRAFREVAVIAGLVERQQPGQRKTGRQVTFSTDLIYDVLRKHEPDHVLIEAAWAD
                     ARARMTDIGRLAALLDRAGAQVRHVRLDRVSPLAVPLLVMIGRESVPQGQADDELLFE
                     AESLAGAAMRLDPAQEDAD"
     gene            complement(4543..4989)
                     /locus_tag="VFV30_11985"
     CDS             complement(4543..4989)
                     /locus_tag="VFV30_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4848856.1"
                     /translation="MSASTARRDPPPFGELNTTPLIDVLLVLLILFILSIPVGQHQVS
                     FDLPSPQPVDSTMELRRENMLSLTAAGTIRWNGTAISEAQLVTLLSAAKLQNPEPLIR
                     FEPEGGAPYGASLRVLNLVKASDPAAFAFSGNKRYAGFAKAEPAPR"
     gene            complement(5136..5588)
                     /locus_tag="VFV30_11990"
     CDS             complement(5136..5588)
                     /locus_tag="VFV30_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4848857.1"
                     /translation="MAMSGGNDDGEPMMEINTTPLIDVMLVLLIMFIITIPVATHAIN
                     IDLPNPSAPPPDVVVKRDKNKVVVMPNNDILWNGSSVNVNQLTGLLKSTLSIKPEPEL
                     QFQPDAQAPYETTAMVLNIIKGSGVTAFGFVGNEQYASFGKAANAKSE"
     gene            complement(5626..6159)
                     /locus_tag="VFV30_11995"
     CDS             complement(5626..6159)
                     /locus_tag="VFV30_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX4848858.1"
                     /translation="MAISMGGGGEEKPMSDINTTPLVDVMLVLLIIFLIAVPVAIQTI
                     QKLKIPVIVSAESKDKVENLLLTVSTTDAAGRSAGDPGFEGASLGGECRIYFNNITPV
                     DSTELQKKAYDRLDAIVKRAGGPEALKNNPELVPQVHIRGDVSAPWRCVAGAVFNIQM
                     AGYPTVGFISTPVDPNG"
     gene            complement(6256..7020)
                     /locus_tag="VFV30_12000"
     CDS             complement(6256..7020)
                     /locus_tag="VFV30_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX4848859.1"
                     /translation="MYFELLQAAAQAAAPQNKFGFKEALEQGGFIAYATVVILGIMSF
                     GSFFILITKWIEQNKILSQHKTLRTTFWRAASLKEGAAKLEKNSAWRQIVDDGIAAEE
                     AHGKMTDALEAHDWLHGSLARSENSINSRLASGLPFLATVGATSPFIGLFGTVVGIYR
                     ALIAIGLAGSASIDKVAGPVGEALIMTALGLLVAVPAVLAYNYLQARNKKIAELLSGF
                     STDVLANINSKGAVKPAVPAAPAAAAKPAAAPAAKQ"
     gene            complement(7092..7757)
                     /locus_tag="VFV30_12005"
     CDS             complement(7092..7757)
                     /locus_tag="VFV30_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4848860.1"
                     /translation="MAYADQEVSGNRIAAFVVVALLHLALGYALVTGLAYEGFKQVMK
                     KVTTVDIKKDEPKKEPPPPPKKAAAAPPIVAPPPKVSLTNVSPPVEITQNIPPPAPPP
                     IVVAPPAPVAAPPPPPAPKFQPKPATPKGNPGNWATTNDYPSRALREEREGVTGFRVS
                     VGPDGRVTGCSVTASSGSSDLDDATCANVTRRARFNPAMDGEGQPTTGSYSGRVRWVI
                     PKD"
     gene            8099..8953
                     /locus_tag="VFV30_12010"
     CDS             8099..8953
                     /locus_tag="VFV30_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848861.1"
                     /translation="MDISQFPIWRAAAAAALALAPAAALADSPPPPPLPSAVLTYADL
                     ADLSDSARLVVRAKPRKVIQVEPERARGLRPGWGRFYVEAKTEAVIAGSEPLGPDLRY
                     LVDLPLDAKGKPVAFKKKSVVLFARTVPGRPGELQLVVPDGQFLWDPLLDSRLRGVLN
                     ALYAPAAPQKVTGVREAIHVSGDLAGEGETQLFLGTANGEPAAVTVARKPGQAPRWSV
                     SFSEVVAGDAAPPARDTLAWYRLACFLPAQLPAAANISPAAADRTAAVRDYRFVMEQL
                     GPCPRSRR"
     gene            9017..>10312
                     /locus_tag="VFV30_12015"
     CDS             9017..>10312
                     /locus_tag="VFV30_12015"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX4848862.1"
                     /translation="MAEPLKIALAGLGTVGIGVIRLVEANAQLIARRAGRPIQITVVS
                     ARDRNKARGVDLSPYAWEDDMVILGERADVDVVVEMVGGSDGPALALARTTIEAGKAL
                     VTANKAMIAHHGLELAAKAEEQKVALKFEAAVAGGIPVIKGLREGAAANEIERVYGIL
                     NGTCNFILTVMEDTGRDFADVLAEAQAKGFAEADPAFDVEGVDAAHKLSILASIAFGS
                     RIDFAAVDASGITRLRAADIAQAEALGYVIRLIGMAETDGEGGAKTLFQRVRAHLVPV
                     DHPLAHVDGATNAVVAEGNFSGRLLFQGAGAGDGPTASAVVADLIDIARGEVGPAFSM
                     PVGELEALGPAETGHRPGRAYVRFMVADRPGVLAEITAALRDAGVSIESLIQKGRAGE
                     AGEVMVALVTHEGPESAVSEALRLLEGSESLSEAPLVLHI"
BASE COUNT         1655 a         3351 c         3650 g         1656 t
ORIGIN      
        1 aaggcgctgg tcgcggccga aaggctcgat ttcggaatag ccttcgacgg cgatggcgac
       61 cggatcggcg cgatcgacgg ccagggccgg gtgatctggg gcgaccagct gctcatgatc
      121 tacgccgaag acctcctgaa gatggagccc ggagccgcga ttatcgcgga tgtgaaggcc
      181 agccgggctc tattcgagcg ggtctctcaa ctcggtggac ggcccactat gtggaaaacc
      241 ggacacagcc tgattaaatc caaaatgaag gagattggtg cgccgctcgc cggtgagatg
      301 agcgggcacg tgttcttcgc ccaccagtat tacgggttcg acgatgcgct ctatgccgcg
      361 atccggctga tcgccgcctc ggctcggctg ggcaagtcgg tgaccgaact gcgcggggcc
      421 atgcccgagc tggtcaacac gccggagctg cgctttcagg tcgacgaaag ccgcaagttc
      481 gcggtgatcg ccgaagtgaa ggagcgcctc tccgcggccg gggccgatgt cgatgccacc
      541 gacggggtgc gagtttcgac cgacgacggc tggtggctgc tgcgcgcctc caacacccag
      601 gacgtgctgg ttgcccgcgc cgagagcgag agcgaagccg ggctggaacg gctgatggcc
      661 cagatcgacg gccagctggc cgccagcgga ctggagcgcg ggccccaagc cgggcactga
      721 caccggagca cggacgccgc gttcgccatc taccagacca ccagcacgcc gccgcccagc
      781 gcggccaggc tggccagcat cgcgctggcg ccgggccagg ccttgcgcca accggcgatg
      841 ctcagcagcg tcgcggtctt gaacagggtg ttgagcgtga ccggcagggc caggaccagc
      901 gccgcgacgc gcgcagtcag ggtgccgggg ggcaggccgc ccatggtgat gatcgccgaa
      961 tcgacgtcca ccgtccccga tatggccagc accgtcgcca gcccggcatc gccatagcgt
     1021 tccagcaccc agcgtgccgc cagggtcagc gccatggtca gcagcattag cagcaccgcc
     1081 gggccaagat cgaacgggtt gcgcagcttc accccgtctt ccagctggcc ggtgccttcg
     1141 ccgcggcggc gcaggatcag ccagcacgcg gccaggctca ccaccatgcc cggcgcggcc
     1201 agcgtggcaa agccgggcag ggcaaaccag gccagcgccg cggtcagcag catgacccgc
     1261 aggaacatgg tggcgctggc cagcgcgacg ccggcgttga ggaccatcgc gtcgcccgcc
     1321 ccgtcgcgca gctttccggc cagcgaggcg gtgaccgcgg tggaggagac catcgaccct
     1381 gccgccgccg tggccagcac gccgcgggcc gggccgagca gccggactgc ggcatagccg
     1441 gcaaaggaaa agcccgaaac cagcaccacc accagccaca gctggcgcgg ccgccaggcg
     1501 ccataggggc ccatcggcgt atcgggcagc agcggcagga tcaccagcgc gatcagcgca
     1561 aagcgggcga tcgccagcat ctcgtgctcg tcgatgtggg tcagccagtg gtgccagcgg
     1621 ctgcgctggg aaaggaccag cacggtcgtc ccggcgagcg cgctggccag ggtgaagtcc
     1681 ccgctgccgg ccaggaaccc gcaggccagg gtaatcagcc cggtcaggct ggcagtgccg
     1741 ctgatcgagg cccggccctt ggtgctgcgc caatagccca gcaccaccag cgcggcggcc
     1801 gcggccagca ggaccgtggc caggccgctg gcgcgttcct gcagcgcgcc ggcaatcccg
     1861 ccggccaggc cgaacagggc gtgggtgcgg atcccggcaa agcggctgcc gggggcgccc
     1921 gtgcggctcg cccagccgcg ctgcacgccg atcagcagcc ccagggccag ggccaggccg
     1981 atcgcggtcg gctcggaata gtcgatcggg tgcatggcct gcttgtggca ctgtccgggc
     2041 gcggaaggaa ggacttggcg ttcgcgcgtt ccgcgcacat cttgggccgg ccatgccaga
     2101 cctgctcccc cccgaactgt ccggctggtt cgccgccaaa ggctggcgcc tgcgccgtca
     2161 ccaggcggaa atgctggagg tggccgcagc cggccgccac gcgctgcttg tggccgatac
     2221 cggcgcgggc aagacgctgg gcgggtttct gccgaccctg gccgatttct gcccgtcgcg
     2281 cctggcgggc catgcgccgc ccgaggggct ccacaccctc tacgtctcgc cgctgaaggc
     2341 gctggcccac gatgtgcagc gcaacctgct cactccggtg gccgatctgg acctggccat
     2401 gcgggttgag gtgcgcagcg gcgatacccc gtccgagcgc aaggcgcgcc agcgcagcaa
     2461 cccgccgcac gtgctgctga ccacgcccga atcgctctcg ctgctgctgt cctatcccga
     2521 ggcggagcac ctgttcgccg ggttgcagcg ggtggtgatc gacgagatcc acgctttcgc
     2581 gccgaccaag cgcggcgatc tgctggccct ggcgctgacg cggctccagg cgctggcgcc
     2641 cggcctgcag cgggtcgggc tgtcggccac tctggccgac ccggccgact ttgcgggctg
     2701 gctcgcgccg gggggcgcgg cggagacggt ttcgctggtc accggcgagc agggcgcggc
     2761 gcccgaactg gcgatcctgc tgcccgacga ggcgcgggtt ccgtggggcg ggcacgccgc
     2821 ggcctgggct gtgccgcagc tgtatgaggc gatccgcgcg aaccgcacga cgctggtgtt
     2881 ctgcaacacg cggtttctgg ctgaattcat ttttcagcag ctgtggaacg ccaacgccga
     2941 tgtcctgccg atcgggatcc accacggctc gctgtccaag gaggcgcggc gcaaggtcga
     3001 aggggcgatg gcccgcggcg agctgcgcgc actggtggca acatccagcc tcgatctggg
     3061 ggtggactgg ggggacatcg acctggtggt gcagatgggg gcgcccaagg gctcctcgcg
     3121 gctgctgcag cggatcgggc gcgccaacca ccggctcgat caggccagcc gcgcgctgct
     3181 ggttcccggc aaccgctttg aattcctcga ggcttttgcg gccgagcagg cggtggagga
     3241 aggccagcgc gacggcgaag cctttcgccc cggcgggctc gatgtgctgg cccagcatgt
     3301 tatggcccgg gcctgcgccg gaccgttcga cggcgaggcc ctgcaccgcg aggtctgctc
     3361 ggcgcggcct tatgcctggg tcgatgccgg gatctgggag cgggtgctgc acttcgttgc
     3421 caccgggggc tatgcgctga aagcctatga ccgttttcag cggatcgtcc gcggccgcga
     3481 cgggctgtgg cggttgaccc atcccgatca cgccgcgcgc ttccggctca acgccgggat
     3541 catcatcgac agcgaaatga tcgaggtgcg gttccgcaac ggccggtcgc tgggccgggt
     3601 ggaggagagc tttgccgcgg cgctgcgccc gggcgatacg atccgctttg ccggactgga
     3661 cctcgaaatc gaggccatgc gcgaggccga actggtggtt cgcgcggcga agaaggcggg
     3721 gcagatcccc agctacatgg gccagcggct gccgctgacc acccatctgg ccgagcgggt
     3781 ccagaccctg ctggccgacc gggccggctg ggcccggttc ccggacgacg tgcgcgaatg
     3841 gctggaagtg caggactggc ggtccgagct gcccggaccc gggcggctgc tggttgagag
     3901 cttcccgcac gagagcaagc actacaccgt gttctactgt ttcgaaggct ggccggccca
     3961 ccagtcgctg ggcatgctga tcacccgccg gatggaggaa cggggcctgg ccccgatggg
     4021 ctttgtcgcc aacgactatg ccctggcggt ctggagcctg cggccggtcg acgatccggc
     4081 gacgctgctt tcacccgata tcctggcgga cgagttcgtt gcctgggtgg aggacagcta
     4141 tctgctgcgc cgggcctttc gcgaagtggc agtgattgcc gggctggtgg agcggcagca
     4201 gccgggccag cgcaagaccg gccggcaagt gaccttctcg accgacctga tctacgacgt
     4261 cctgcgcaag cacgagccgg accatgtgct gatcgaggcg gcctgggccg atgcccgggc
     4321 ccggatgacc gacatcggcc ggctggccgc gctgctggac cgggccgggg cgcaggtgcg
     4381 gcatgttcgg ctggaccggg tcagcccgct ggccgtgccg cttctggtca tgatcgggcg
     4441 tgaaagcgtg ccgcaggggc aggccgacga cgagctgctg ttcgaagcgg aaagcctggc
     4501 cggggcggcg atgcggcttg acccggcgca ggaggacgcg gactagcgcg gggccggttc
     4561 ggccttggca aacccggcat agcgcttgtt gcccgaaaag gcgaaggcgg caggatcgct
     4621 ggccttgacc aggttcagca cccgcagcga ggcgccatag ggggcgccgc cctcgggctc
     4681 gaagcggatc agcggttcgg ggttctgcag cttggccgcg ctcagcaggg tgacgagttg
     4741 ggcctcgctg atggcggtgc cgttccagcg gatcgtgccc gcggcggtca acgagagcat
     4801 gttctcgcgc cgcagctcca tcgtcgaatc gaccggttgc ggggaaggca gatcgaacga
     4861 gacttggtgc tggccgaccg ggatcgacag gatgaacagg atcagcagga ccagcaggac
     4921 atcgatcagc ggggtggtgt tgagctcgcc gaatggcggc ggatcgcggc gggcggttga
     4981 tgcagacaca ggcacctcct tcgcggatga gaaggagata gtataacatc aagtggatct
     5041 gtcaaggtgg aggtacctgg caagcgggca acgaaaaagg ggcggatcgc tccgcccctt
     5101 tgtcttgttc cgtctggcgg cccgaaggcc tgcccttatt cggacttggc gttggccgcc
     5161 ttgccgaagc tggcatactg ttcgttgccg acgaaaccga aggccgtgac ccccgaaccc
     5221 ttgatgatgt tgagcaccat cgcagtggtt tcatagggtg cctgggcatc cggctggaac
     5281 tgcagttccg gttccggctt gatcgacagg gtcgacttca gcaggccggt cagctggttc
     5341 acgttcaccg acgaaccgtt ccagaggatg tcgttgttcg gcatcaccac gaccttgttc
     5401 ttgtcgcgct tgaccacgac atccggcggc ggggccgacg ggttgggaag gtcgatgttg
     5461 atggcgtgag tggccacggg gatggtgatg atgaacatga tgaggagaac gagcatgacg
     5521 tcgataagcg gcgtcgtgtt gatctccatc atcggctcgc catcgtcatt gccgcctgac
     5581 attgccatgg tgggttactc ctgaaaggtg tctgtccgtc cggcctcagc cgttcggatc
     5641 gaccggggtc gagatgaagc cgacggtcgg ataaccggcc atctggatgt tgaagaccgc
     5701 gccggcaaca caccgccagg gtgcgctcac gtcgccgcga atgtggacct gcgggaccag
     5761 ctcaggattg ttcttgagcg cttcgggccc accggcacgc ttcacgattg catcgagacg
     5821 gtcgtaagcc ttcttctgca gttcggtcga gtccaccgga gtgatgttgt tgaagtagat
     5881 ccggcattca ccacccagcg aagcaccctc gaagcccggg tcacccgcgc tgcgtccggc
     5941 agcatcggtc gtgctgacgg tgagcaggag gttttcgacc ttgtccttcg attccgccga
     6001 aacaatgacg ggaatcttga gcttctggat cgtctggatg gcaaccggaa ccgcgatgag
     6061 gaagatgatc aggagcacca gcatcacgtc gacaagcggc gtggtgttga tgtccgacat
     6121 cggcttttcc tcgccgccgc ctcccatcga aatcgccatg ggctaattcc tattcctagc
     6181 tttacctcgg accgggcaga gcccgggccg gaaaggggcc ggcgcgtccc gcgggaagcg
     6241 ccggcctggc gatcattact gcttggcggc cggagcggca gccggcttgg cagcggcagc
     6301 cggagcagcc ggaaccgcag gcttcaccgc gcccttcgag ttgatgttgg ccagaacgtc
     6361 ggtcgagaag cccgaaagca gctcggcgat cttcttgttg cgcgcctgca ggtagttgta
     6421 ggcgagcacg gccggaacgg cgacgagcag accgagcgcg gtcatgatca gcgcttcacc
     6481 gaccgggccg gcgaccttgt cgatcgaagc cgaaccggcg aggccgatgg cgatcagcgc
     6541 gcggtagatc ccgacgacgg tcccgaacag accgatgaac ggcgaggtcg caccgacggt
     6601 ggcgaggaac ggcaggcccg aagcgagacg cgagttgatc gagttttccg agcgagccag
     6661 cgagccgtgc agccagtcat gggcttccag agcgtcggtc atcttgccgt gggcttcttc
     6721 ggcggcgatg ccgtcgtcca cgatctggcg ccaggcgctg ttcttctcga gcttggcagc
     6781 gccttccttg aggctggccg cgcgccagaa ggtggtgcgc agggtcttgt gctggctcag
     6841 gatcttgttc tgctcgatcc acttggtgat caggatgaag aacgagccga acgacatgat
     6901 gccgaggatg acgacggtgg cgtaagcgat gaagccaccc tgttcgagag cttccttgaa
     6961 gccgaacttg ttctgcggag cggctgcctg cgcagcggcc tggagaagtt caaagtacat
     7021 gcgatagtcc tctcaagagc aataagttga aaccagatgg cccggtgggc cgaaaccttc
     7081 gattaccgtg attaatcctt cgggatcacc cagcgcacgc ggcccgaata ggaaccggtc
     7141 gtcggctgtc cttcaccgtc catcgccgga ttgaaccggg cccggcgcgt cacgttggcg
     7201 caggtggcgt catccaggtc ggagctgccg ctcgatgcag tgaccgagca gccagtgaca
     7261 cgtccgtcgg gaccgacgct gacgcggaaa ccggtcacgc cttcgcgctc ttcgcgcagg
     7321 gcccgcgagg ggtaatcgtt ggtcgtagcc cagttgccgg gattgccctt gggcgtggcc
     7381 ggcttcggct ggaacttcgg agccggcggc ggcggcggcg cagcgaccgg ggccggcgga
     7441 gcgaccacga tcggcggcgg agccggcggc gggatgttct gcgtgatttc caccggcggc
     7501 gaaacgttgg tcaggctgac cttcggcggc ggagccacga tcgggggcgc agcggcagcc
     7561 ttcttaggcg gcggcggcgg ctccttcttg ggttcatcct tcttgatgtc gacggtcgtt
     7621 accttcttca tcacctgttt gaacccttcg taggccaggc cggtaaccag cgcatagcca
     7681 agggccaggt gcaaaagggc aacgacgacg aaggcggcaa ttcgattacc gcttacttct
     7741 tggtcagcgt aagccattct gacacatcac tcctaaaaca gtccccccgg gacaaggccc
     7801 ggacccgacg ccaaacctgt tgcgagagaa tcgcatcagg ccagacgccc agattgttag
     7861 tttccaccct gcgggtcaat ccgggaaacc ggacggacgc ccgaaccgcc agattgcggg
     7921 ccgttgttat gctttggccc gattttcggg agctttaacc gcccccgccg ccacacgcaa
     7981 cgctttatcc attaacccgg gattcactta tgttagggtt tttccctggt ggtgcgggcg
     8041 ctgaatcccg cgctttcggc agcttgccgc caggcacaac agcgggcaat gaggcgttat
     8101 ggacatctcc caattcccga tttggcgcgc ggccgcagcg gccgccctgg ccctggcacc
     8161 ggctgcggcc ctggctgatt cgccgccgcc gccgcctctg cccagcgccg tgctgaccta
     8221 tgccgacctt gccgacctgt ccgattcggc ccgtctggtg gtgcgggcca agccgcgcaa
     8281 ggtgatccag gtcgagcccg aacgggcccg cggcctgcgc ccgggctggg ggcgtttcta
     8341 tgtcgaggcc aagaccgaag cggtgatcgc cgggtccgag ccgctgggcc ctgacctgcg
     8401 ctatctggtc gacctgccgc tcgacgcgaa gggcaagccg gtcgcgttca agaagaagag
     8461 cgtggtgctg ttcgcccgca ccgtccccgg ccgtccgggc gaactgcagc tggtggtgcc
     8521 cgatggccag ttcctgtggg acccgctgct cgacagccgg ctgcggggcg tgctgaacgc
     8581 gctttatgcc ccggcggctc cgcagaaggt caccggcgtg cgcgaggcga tccacgtcag
     8641 cggcgatctg gccggcgaag gggaaaccca gctgttcctg ggcaccgcca acggcgaacc
     8701 ggcggcagtc accgtcgccc gcaagccggg tcaggcgccg cgctggtcgg tctcgttttc
     8761 ggaagtggtg gccggcgatg ccgcgccccc ggcgcgcgac acgctggcct ggtaccgcct
     8821 ggcctgcttt ctcccggccc agctgccggc cgcggccaac atttccccgg cggcggccga
     8881 tcgcactgcc gcggtgcgcg actaccgctt cgtgatggag cagctcggcc cctgcccgcg
     8941 cagccggcgc tagccccttt cgggcttgcc gcctgcgcct ggggtgccta aggcccggcc
     9001 ccggaaaggg gcgaaaatgg ccgaaccgct gaagattgcg cttgccggat tggggaccgt
     9061 ggggatcggg gtgatccgcc tggtggaggc caacgcccag ctgattgccc gccgcgcggg
     9121 ccggccgatc cagatcacgg tggtttccgc gcgcgatcgc aacaaggcgc gcggggtcga
     9181 tctgtcgccc tatgcctggg aagacgacat ggtgatcctg ggcgagcggg cggatgtcga
     9241 cgtggtggtg gagatggtcg gcggctcgga cgggccggcc ctggcgctcg cccgtaccac
     9301 gatcgaggcg ggcaaggcgc tcgtcaccgc caacaaggcg atgatcgccc accacgggct
     9361 ggaactggcc gccaaggccg aagagcagaa ggtggcgctg aagttcgaag ccgcggtggc
     9421 cggcgggatc ccggtgatca agggcctgcg cgaaggcgcg gcggcgaacg agatcgagcg
     9481 ggtctacggc attctcaacg gcacctgcaa tttcatcctg acggtgatgg aagacaccgg
     9541 ccgcgatttc gccgacgtgc tggccgaggc ccaggccaag ggctttgccg aagccgatcc
     9601 ggccttcgac gtcgaagggg tcgatgcggc gcacaagctc tcgatcctgg cctcgatcgc
     9661 attcggctcg cggatcgatt tcgccgcggt cgatgccagc gggatcaccc gcctgcgcgc
     9721 tgccgacatc gctcaggccg aagcgctggg ctatgtcatc cgcctgatcg gcatggccga
     9781 gactgatggc gagggcgggg ccaagaccct gttccagcgc gtccgcgcgc acctggtccc
     9841 ggtcgatcac ccgctggccc acgttgacgg ggcgaccaac gcggtggtgg ccgagggtaa
     9901 tttctcgggc cggctgctgt tccagggcgc cggggccggc gacgggccga ccgccagcgc
     9961 cgtggtggcc gacctgatcg acattgcccg cggcgaagtg ggcccggcct tctcgatgcc
    10021 ggtgggcgag ctggaagccc tgggcccggc cgagaccggc caccgcccgg gccgcgccta
    10081 tgtccgcttc atggttgcgg accggcccgg cgtgctggcg gaaatcaccg ccgcgctgcg
    10141 cgatgccggg gtttcgatcg aaagcctgat ccagaagggc cgtgcgggcg aagctggcga
    10201 ggtaatggtc gcgctggtga cccacgaagg tccggaaagc gcggtcagcg aggcgctgcg
    10261 gcttctggaa ggatccgaaa gcctgagcga ggccccgctg gtgctgcata tc
//
LOCUS       DASUDV010000547         6777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_416948,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000547 DASUDV010000000
VERSION     DASUDV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416948"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFV30_12020"
     CDS             complement(<1..675)
                     /locus_tag="VFV30_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEX4848863.1"
                     /translation="MTKQTKIPVPEQEKPKADSATLIERVVRNYDLVKLAPAPIPENL
                     IPPVSKRRKYRRADDAIEDAVTSPVEPEAPAAKPEPVKPAPAAPVAEAPVEAVRPEPR
                     PEVVEPEVIAPVRNVPAPVAVPVRFKGERHPVNRQHLREQGLIVPEGSVTALLEEFRI
                     VKRQLLYNASQLAQHGLGPAAQRILICSPHPGEGKSFTSVNLALAIAAEKESEVLLVD
                     ADFAKPS"
     gene            complement(686..2209)
                     /locus_tag="VFV30_12025"
     CDS             complement(686..2209)
                     /locus_tag="VFV30_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEX4848864.1"
                     /translation="MNNVYEEIRSAVYSVWHRRWLALGVAWAVCLLGWLVVSMIPNSY
                     ESKARIYVKLEDVLSDVTGVPTSNKRDIERVQQTLTSTINLEKVIRGTPIGAEITSPK
                     EMENAVLSLGKKIKVESTKDSTFEITASYGNGGNSDAANAKIAQAIVQKLIDLFREEN
                     LAGNRGEVTTSLDFMDQQLASRQKELEAAEQKRLAFEAANPSFAKDGPSPVQRLETLR
                     AELRGIDADLAAAQSALAAINGQLAGTPRTLPGQGAAGGARGALAEAQGNLASLRARG
                     LTENHPDVIAARNQVNMLRQAAASEGNSGPGGQPNPAYSTLETMKVERQANVQSLTAR
                     RASVQAEINQITAQQFSNPEAAAEQQRINRDYDVLKQQYDKLLQDREELRLRGQVETE
                     HSGVKFQVVDPPSTPRSPVSPNRPILLLGVLFVGLGAGAGVAFAVGQLRSTFATTAKL
                     ERTLGLPVLGAISQTLNDSAKALRKKRLQYFYAASAGLGAVFALLLTLEFVQRGMVA"
     gene            complement(2235..2879)
                     /locus_tag="VFV30_12030"
     CDS             complement(2235..2879)
                     /locus_tag="VFV30_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEX4848865.1"
                     /translation="MPINRFSRLLAGSAMLSLMLGGCAGGASGPQLPPASFVAMQEGP
                     GEDYIIGPLDELTIFVWRNPELGAKVQVRPDGRITTPLITDMPAVGKTPKMLADDITL
                     QLSQYIQDPLVSVIVNKFAGTYSQQVRIVGATDKPASVPYRANMTLLDAMIAVGGLSE
                     FAAGNRAKLIRFDKTTGRQKEYSIRLGDLLKKGDSRANVMLMPGDVIIIPESTF"
     gene            complement(3057..4283)
                     /locus_tag="VFV30_12035"
     CDS             complement(3057..4283)
                     /locus_tag="VFV30_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HEX4848866.1"
                     /translation="MKPLGAIPAGFEALSGELAIAGRTASELVEEAGGTPLFVYSRAH
                     LQSRVAALRAAMPARLAIHYAMKANPYGPLLQEMAGLVDGFDVASAGELTLAQAAGLD
                     ARRISFAGPGKRTEELEAAISAGVTINLESEGEAARALAAGERLGITPRLAVRVNPDF
                     DLKGSGMKMGGGAKQFGVDAERVPALVRQVIAAGAEWRGFHIFAGSQALDAAALIETQ
                     SQTLALAARLADESGAALPHCNLGGGFGIPYFPGDEPVDIAAIGQALGERFAQLPASL
                     AETRFCIELGRYLVGEAGVYLTRIVDRKVSHGEVFLIVDGGLHHQLAASGNFGTVVRR
                     NYPVAIASRFGAPTEEEASVVGCLCTPLDRLADKAGFPAADEGDLVAVFCAGAYGASA
                     SPAQFLGHGPAKEMLV"
     gene            complement(4280..5779)
                     /locus_tag="VFV30_12040"
     CDS             complement(4280..5779)
                     /locus_tag="VFV30_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HEX4848867.1"
                     /translation="MRPLDHLALRGADAAPALVLREGPLSYKDLRDRVARMAGWLRTQ
                     VPEAGARVATWAAKGELTCLMPLAAARAGLVHVPINPLLKRAQVAHILADSGSRLLLA
                     TPSRLAGLEEGDIPAGCLAVTEADGIEQARSAPALDPAEADPEALAAILYTSGSTGKP
                     KGVMLSHANMWLGAESVASYLGLAADDVTLAVLPLSFDYGQNQLLSTWHAGGCVVPLD
                     YLTPRDVVKAVARHGVTTLAAVPPLWVQVAELDWPPEAAANLRRLTNSGGALTVDLVR
                     RLRGLFPDARLFAMYGLTEAFRSTFLDPALIDSHPTSMGKAIPHAEILVIGDDGAVTA
                     DGEEGELVHCGPLVAHGYWQDPVRTAERFKPAPAASRYGGMAVWSGDRVRRDADGLLY
                     FVGRRDAMIKSAGNRISPQEIEEAALATGLVAEAVALGVPDERLGHAVHLIVRAAPGT
                     SHAEEELPRMLLKELPNFMQPKVIHWRVAMPISPNGKIDRVGLYQELAA"
     gene            5840..>6777
                     /locus_tag="VFV30_12045"
     CDS             5840..>6777
                     /locus_tag="VFV30_12045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4848868.1"
                     /translation="MVSVVYHDDLKEVQADAPLAALVSAAQARAPFDRIEWWQGLVAQ
                     CGILPLIAVARDGKHRAVLPLMRQARRIHALGNWYTFRTAPLFTPGADADCLLTALAE
                     DLAGESPHLVLFPLPDENGETTRFARALRQAGWAVFVSPCDVNHVLPVGGRSFAEYLA
                     TRPGPLRTTLKRKAGKVEVELATVFDPALWAEYEAVYAASWKPEEGSPAFLRAFAEAE
                     GQAGRLRMAVARAEGQAVAAQFWTVEGGTAFIHKLAHTEASKPLSPGTTLSAALFEHV
                     IDRDKVDLVDFGTGNDSYKRDWMEQVRPRYRIEALRP"
BASE COUNT         1054 a         2308 c         2278 g         1137 t
ORIGIN      
        1 cgaaggcttg gcaaagtcgg catcgaccag cagcacttcg ctttccttct cggcagcgat
       61 ggccagggcc aggttgaccg aggtgaagct cttgccctcg cccggatgcg gcgagcagat
      121 caggatccgc tgcgccgccg gacccagccc gtgctgggcc agctggctgg cgttgtagag
      181 caactggcgc ttcacgatcc ggaactcttc cagcaacgcg gtgacgctgc cttcgggcac
      241 gatcaggccc tgttcgcgca ggtgctggcg gttgaccgga tggcgctcgc ccttgaagcg
      301 gaccggcacc gcgaccggag ccggcacgtt gcggaccggc gcgatcactt cgggctcgac
      361 cacttcgggc cgcggttcgg ggcgaaccgc ctcgaccggc gcttcggcaa ccggagcggc
      421 cggggccggc ttgaccggtt ccggcttggc agcgggtgct tcgggttcga ccgggctggt
      481 caccgcatcc tcgatcgcat cgtcggcgcg gcggtacttg cggcgcttgg acacgggcgg
      541 gatcaggttt tccgggatcg gcgccggggc cagcttgacc aggtcgtaat tgcgaacgac
      601 ccgctcgatc aacgtcgcac tgtcggcctt gggcttttcc tgctcgggca cggggatctt
      661 ggtttgcttg gtcatttcga tcccctcagg cgaccatgcc gcgctggacg aattcgagcg
      721 tgagcaggag cgcgaagacg gctcccagcc cggccgaggc ggcatagaag tactgcaacc
      781 gcttcttgcg cagcgccttg gcactgtcgt tcagcgtctg gctgatcgcg ccgaggaccg
      841 gcaggcccag ggtccgctcc agcttggcgg tggtggcaaa ggtcgagcgc agctggccga
      901 cggcgaaggc aacgccggca cccgcgccca ggcccacaaa cagcacgccc agcagcagaa
      961 tcggacggtt cggcgaaacc gggctgcgcg gggtgctcgg cggatcgacc acctggaact
     1021 tcacgccgct gtgctcggtt tccacctggc cgcgcaggcg cagctcttcg cggtcctgca
     1081 gcagcttgtc gtactgctgc ttgagcacgt cgtaatcgcg gttgatgcgc tgctgttcgg
     1141 ccgccgcttc ggggttgctg aactgctgcg cggtgatctg gttgatctcg gcctggaccg
     1201 aggcgcgccg cgcggtcagc gactggacat tggcctgacg ctcaaccttc atcgtttcca
     1261 gcgtcgaata ggccgggttg ggctggccgc ccgggccgct gttgccttcg gacgccgccg
     1321 cctggcgcag catgttgacc tggttgcgcg ccgcaatcac gtcgggatgg ttttcggtca
     1381 gtccccgggc ccgcaacgag gcgagattgc cctgggcttc ggccagggcg ccgcgggcgc
     1441 cgccggccgc gccctgtccg ggcagggtgc ggggcgttcc ggccagctgg ccgttgatcg
     1501 ccgccagcgc gctttgcgcc gcggccagat cggcatcgat cccgcgcagt tcggcgcgca
     1561 gcgtttcgag ccgctggacc ggcgacggac cgtctttcgc gaacgagggg ttggccgctt
     1621 caaaggccag ccgcttctgc tcggcggctt ccagttcctt ctgccgcgag gcgagctgct
     1681 ggtccatgaa gtcgagcgag gtggtcacct cgccgcggtt tccggccagg ttctcttcgc
     1741 ggaacaggtc gatcagcttc tggacgatcg cctgggcgat cttggcgttg gccgcatcgg
     1801 aattgccacc gttgccatag ctggcggtga tttcgaaggt gctgtccttg gtgctttcga
     1861 ccttgatctt cttgcccagg ctcaggaccg cgttctccat ctccttgggg ctggtgatct
     1921 cggccccgat cggagtgccg cggatcacct tttccaggtt gatggtgctg gtcagggtct
     1981 gctggacccg ctcgatatcg cgcttgttgc tggtcggcac gccggtcaca tcggacagca
     2041 cgtcttccag cttcacatag atgcgcgcct tcgattcata gctgttgggg atcatcgaga
     2101 ccaccagcca gccaagcagg cagaccgccc aggccacgcc aagcgcaagc cagcggcggt
     2161 gccagaccga atagaccgcc gagcggattt cttcgtaaac gttgttcatc agctgccccc
     2221 gggccgtcct ttccttagaa cgtgctttca gggatgatga tgacgtcgcc cggcatcagc
     2281 atgacattgg cgcggctgtc gcccttcttg agcaggtcgc ccaggcggat cgaatattcc
     2341 ttctggcgcc cggtcgtctt gtcgaagcgg atcagcttgg cccggttgcc ggccgcgaac
     2401 tcgctcagcc cgccgacggc gatcatcgcg tcgagcaggg tcatgttggc gcggtagggc
     2461 accgaggccg gcttgtcggt cgcgccgacg atccggacct gctggctgta agtgccggca
     2521 aacttgttga cgatcaccga gaccagcgga tcctggatgt actgcgaaag ctgcagggtg
     2581 atgtcgtcgg ccagcatctt gggcgtcttg cccacggccg gcatgtcggt gatcagcgga
     2641 gtggtgatgc gcccgtcggg ccgcacctgg accttcgcgc ccagttccgg attgcgccag
     2701 acgaagatcg tcagctcgtc cagcggaccg atgatgtaat cctcgcccgg gccttcctgc
     2761 atcgccacga acgacgcggg cggcagttgc gggccgctgg ctccaccggc gcagccaccc
     2821 agcatcaggc tgagcatcgc gctaccggcc agcaggcggg aaaaacggtt gatcggcatc
     2881 gggcattccc ttgtcaggtg ccgcgccgcg ggatcaggca tgccctaccc gaccagcgac
     2941 ccggctgtgt gagcctccgc tatcgggaaa aagggtgaat attgcgttag ccctatgtgc
     3001 agattcggtg tgcacgaagg gcgggtccca tttcgggacg ggccgtatcg gggttgttaa
     3061 accagcattt ccttcgccgg accgtgcccc aggaactgcg ccgggctggc gctggcgcca
     3121 taggccccgg cgcagaacac cgcgaccaga tcgccctcgt ccgcagcggg gaaaccggcc
     3181 ttgtcggcca gccgatccag cggcgtgcac aggcagccaa ccacgctggc ctcttcctcg
     3241 gtcggcgcgc cgaaccggct ggcaatcgcc accggatagt tgcggcgcac cacggtgccg
     3301 aaattgcccg atgcggccag ctggtgatgc aggccgccgt caacgatcag gaacacctcg
     3361 ccgtggctga ccttgcggtc gacgatccgg gtcagataga cccccgcctc gcccaccaga
     3421 tagcgtccca gttcgatgca gaagcgagtc tcggccaggc tggccggaag ctgcgcgaag
     3481 cgctcgccca aggcctgacc aattgctgca atatcaacgg gttcgtcgcc tgggaaatag
     3541 ggaattccaa agccgccgcc gaggttgcaa tgcggcaggg cggcaccgga ttcgtcggcc
     3601 agccgcgcgg ccagggccag ggtttgcgac tgggtttcga tcagcgccgc ggcgtccagc
     3661 gcctggctgc cggcaaagat gtgaaagccg cgccattcgg ctccggcggc gatcacctgg
     3721 cggaccagcg ccggcacccg ctcggcatcg acgccgaact gcttggcccc gccgcccatc
     3781 ttcatgcccg atcccttgag atcgaaatcg ggattgaccc gcaccgcgag gcgcggggtg
     3841 atccccagcc gctcccccgc tgcaagtgcg cgagcggctt cgccttcgga ttccaggttg
     3901 atggtcaccc cggccgaaat cgccgcttcc agctcctcgg tccgcttgcc cggtccggca
     3961 aagctgatcc gtcgggcatc gagaccggcc gcctgggcca acgtcagctc cccggccgag
     4021 gcgacatcga agccgtcgac cagcccggcc atttcctgca gcagcgggcc ataggggttc
     4081 gccttcatgg cgtaatggat cgccagccgc gccggcatgg ccgcgcgcag agccgccacg
     4141 cggctttgca ggtgcgcacg cgaatagacg aacagcggcg ttccgccagc ttcctcgacc
     4201 agctcggacg cggtgcgccc ggcgatcgcc agctcgcccg aaagcgcttc gaacccggcc
     4261 gggatcgcac ccagcggctt catgccgcga gctcctggta cagcccgacc cggtcgatct
     4321 tgccgttggg cgagatcggc atggccacgc gccagtggat caccttgggc tgcatgaaat
     4381 tgggcagttc cttgagcagc atacgcggca attcttcctc cgcatgtgaa gttccgggag
     4441 ccgcccggac aatcaggtgg accgcatggc caagccgttc gtccggaacg cccagcgcaa
     4501 ccgcttcggc aaccaggccg gtcgccaggg ccgcttcctc gatttcctgc gggctgattc
     4561 ggttgccggc gcttttgatc atcgcgtcgc gccggccgac gaaataaagc aggccgtccg
     4621 catcgcgccg cacccggtcg cccgaccaga cggccatgcc gccatagcgc gaagccgccg
     4681 gtgcgggctt gaaccgctcg gccgtgcgca cgggatcctg ccaatagccg tgggccacca
     4741 gcggtccgca gtggaccagt tcgccctcct cgccgtctgc ggtcacggcg ccgtcgtcac
     4801 cgatcaccag gatctcggca tggggaatgg ccttgcccat tgaggtcggg tggctgtcga
     4861 tcagcgcggg atcgaggaag gtggagcgga acgcctcggt caggccgtac atcgcgaaga
     4921 gccgcgcatc ggggaacagg ccgcgcaggc ggcgaaccag atcgactgtc agcgcgccgc
     4981 cggaattggt cagccggcgc agattggcgg cggcttcggg cggccagtcc agctcggcca
     5041 cctggaccca cagcggcggg acagcggcca gcgtggtcac gccgtgccgg gccaccgcct
     5101 tcaccacgtc gcgcggggtc agatagtcca gcggcaccac acagccccca gcatgccagg
     5161 tcgaaagcag ctggttctgg ccgtagtcga acgacagcgg cagcacggcc agcgtcacat
     5221 cgtccgcggc aaggcccaga taggatgcca cgctttccgc acccagccac atgttggcgt
     5281 ggctcagcat cacccccttg ggcttgccgg tggatccgct ggtatagagg atcgccgcca
     5341 gcgcttcggg atcggcctcg gccggatcca gcgccggggc gctgcgggcc tgctcgatcc
     5401 catcggcctc ggtaacagca aggcagccgg cggggatgtc cccctcctcc agcccggcaa
     5461 ggcgcgacgg tgtggcgagc agcaggcgcg agccgctgtc ggccaggata tgcgccacct
     5521 gggcccgctt cagcagcggg ttgatcggta cgtggaccag cccggcccgg gccgcggcca
     5581 gcggcatcag gcaggtcagc tcgcccttgg ccgcccaggt cgccacccgc gcaccggctt
     5641 cgggcacctg ggtgcggagc catccggcca tgcgggcgac acgatccctt aagtccttgt
     5701 agctaagagg gccttcgcgc agcaccaaag ctggcgcagc atccgcgcca cgcaaggcga
     5761 gatggtctag cggccgaatc gtcttgggat ggtgggaaga cacgggtttc aggggctcca
     5821 aggggcaagg atcaagcggt tggtttcggt cgtttaccac gatgatctta aagaagtgca
     5881 agccgatgcg ccgctggccg cattggtcag cgccgcgcag gcccgcgcgc cctttgaccg
     5941 gatcgaatgg tggcaggggc tggtcgcgca gtgcggcatc ctgcccctga tcgccgtcgc
     6001 ccgcgatgga aagcaccgcg cggtcctgcc gctgatgcgc caggcccggc ggattcacgc
     6061 gctgggcaac tggtacacct tccgcaccgc gccgctgttt actcccggcg ccgatgccga
     6121 ctgcctgctg accgcgctgg ccgaagacct tgccggggaa agcccgcacc tggtgctgtt
     6181 tccactgccg gacgagaacg gcgagaccac ccgcttcgcc agggccctgc gccaggccgg
     6241 ctgggcggtg ttcgtcagcc cgtgcgacgt aaaccatgtg ctgccggtcg gcgggcgcag
     6301 ctttgccgaa tacctggcga cccgcccggg gccgctgcgc accacgctga agcgcaaggc
     6361 cggcaaggtg gaggtcgaac tggccaccgt cttcgatccc gcgctgtggg ccgaatacga
     6421 ggcggtctat gccgctagct ggaagcccga ggaaggatcg cccgcgttcc tgcgcgcctt
     6481 tgccgaggcc gagggccagg ccggacggct gcgaatggcc gtggcccgcg ccgagggcca
     6541 ggccgtcgcc gcccagttct ggacggtcga gggcgggacc gccttcattc acaagctggc
     6601 ccataccgag gccagcaagc cgctgtcgcc cggcaccacc ctgtccgccg cgctgttcga
     6661 acacgtgata gaccgcgaca aggtcgatct cgtcgatttc ggcaccggca acgacagcta
     6721 caagcgcgac tggatggaac aggtccggcc gcgctaccgg atcgaagccc tgcgtcc
//
LOCUS       DASUDV010000548         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1808936,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000548 DASUDV010000000
VERSION     DASUDV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808936"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VFV30_12050"
     CDS             <1..1298
                     /locus_tag="VFV30_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848869.1"
                     /translation="EQKATIDANQASTLGQRWYVLIIMMLVYAISIADRYVLSTLLGP
                     ISKEFSLSDTETAWLGIPLALFYVVMGIPLSWLCDRTNRTRLLAASVAVWSAMTAITG
                     YTKGYFDLLLARVGVGIGEAGGTPACNAIIADYFPADRRSMAMTVFALGAPIGAWLGS
                     DIAGYVSIEHGWRAAFLVLGVPGILLALIILVTIKEPQRGRLDAVTEEKAPTVVETLK
                     FLWSQKAAVHALWASGLSAFWGWGLMWFTPLFLQRTYGMNESEAGGLLGWIYMFGGIG
                     ASLITAWIVARPAYTDPRKIARLLAVVTALATIPSFLAYYTYDLGVAKLMWWLFIPAI
                     YFYIGPAFALCQNLSSPRMRAMTVAISLLIANVLNLIVAPWIVGSLSDYFAGPGGADA
                     ASLRLAQLILAPAGLWAAFHYWRAEKYIVEDQKRAIGYV"
     gene            1291..>2169
                     /locus_tag="VFV30_12055"
     CDS             1291..>2169
                     /locus_tag="VFV30_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4848870.1"
                     /translation="MSEPLRSYIAGDWILPDPANGEHAAINPATEEVCARVALCGPAE
                     ADAAVRAARAAFDGWSTTPLEQRIALVEALIAVFQRRYDEMVVAITTEMGAPHDLSRN
                     SQAECGPGHLAETVRAAREFVWERPIGSGALLVHEAVGVCVLITPWNWPINQLAAKIG
                     PALIAGCTMVVKPSEVAPLSAQLFAEFVAEAGFPAGVFNLVHGTGAGVGPTLTAHPEV
                     DMVSFTGSTAAGILVSKAAADTVKRVSLELGGKSPNIVFDDAGLDAAVTRGVRHCFNN
                     TGQSCNAPTRMLVEQSA"
BASE COUNT          324 a          712 c          729 g          404 t
ORIGIN      
        1 tggagcaaaa ggcgacaatc gatgccaatc aggcatcgac gctgggtcag cgctggtacg
       61 tgctgatcat catgatgctg gtttacgcga tcagcattgc cgatcgctac gtgctgtcca
      121 ccctgcttgg gccgatcagc aaggaattca gcctgagcga caccgagacc gcctggctcg
      181 gcattccgct ggcgctgttc tatgtcgtga tgggcatccc gctgtcgtgg ctgtgtgacc
      241 gaaccaaccg gacccggctg ctggccgcct cggttgcggt ctggtcggcg atgaccgcga
      301 tcaccggcta caccaagggc tacttcgacc tgctgctggc ccgcgtcggt gtcggcatcg
      361 gcgaagcggg cggcaccccg gcctgcaacg ccatcatcgc tgattacttc cccgccgacc
      421 gccgctcgat ggcgatgacc gtttttgccc tcggcgcacc gatcggcgcc tggctgggtt
      481 ccgatattgc gggttatgtt tccatcgaac acggctggcg cgcggcgttc ctcgtgctgg
      541 gcgtgccggg cattctgctg gccctgatca ttctggtgac catcaaggag ccgcagcgcg
      601 gccgtcttga cgcggtgacc gaggaaaagg cgccgaccgt tgtcgaaacg ctgaagttcc
      661 tgtggtcgca gaaggcggcg gtgcacgcgc tctgggccag tggcctctcc gccttctggg
      721 gctggggcct gatgtggttc accccgctgt tcctgcagcg gacctatggc atgaacgaaa
      781 gcgaagcggg cggtctgctc ggctggatct acatgttcgg cgggatcggt gcttcgctga
      841 tcaccgcctg gatcgtggcc cgcccggcct ataccgatcc ccgcaagatc gcccgtctgc
      901 tggccgtcgt gaccgcgctg gcgacgatcc cctcgttcct ggcctattac acctacgatc
      961 tgggcgtggc caagctgatg tggtggctgt tcatcccggc gatctatttc tacatcggcc
     1021 ccgcctttgc gctgtgccag aacctgtcct cgccgcggat gcgggcgatg accgtggcga
     1081 tctcgctgct gatcgccaac gtcctcaacc tgatcgtcgc tccgtggatc gtcggctcgc
     1141 tgagcgatta cttcgccggt cccggcggag cggacgcggc atcgctgcgg ctggcccagc
     1201 tgatcctggc cccggcgggc ctgtgggcgg cgttccacta ttggcgcgca gaaaagtaca
     1261 tcgtcgagga ccagaagcgg gcgatcggtt atgtctgagc cgctgaggtc ctacattgcc
     1321 ggggactgga tcctgccgga tccggccaac ggtgagcacg cggcgatcaa tcccgccacc
     1381 gaagaggtct gcgcccgggt cgccctgtgc ggcccggccg aggccgatgc ggcggtgcgg
     1441 gctgcgcgcg ccgcgtttga cgggtggagc accaccccgc ttgagcagcg gattgcgctg
     1501 gtcgaggcgc tgatcgcggt gttccagcgc cgctatgacg aaatggtcgt ggcgatcacc
     1561 accgaaatgg gggcgccgca cgatctgtcg cgcaattcgc aggcggaatg cggccccggc
     1621 cacctggccg aaacagtccg cgccgcgcgc gaattcgtgt gggaacggcc gatcggatcg
     1681 ggcgccctgc tggtgcatga agcggtgggc gtctgcgtgc tgatcacgcc gtggaactgg
     1741 ccgatcaacc agctggcagc caagattggc ccggccctga tcgccggctg caccatggtg
     1801 gtaaagccca gcgaagtggc cccgctttcg gcccagcttt tcgcggaatt cgtggccgag
     1861 gccgggttcc ccgccggagt gttcaacctg gttcacggca ccggcgccgg tgtcggcccc
     1921 accctgaccg cccatcccga ggtcgacatg gtgtccttca ccggatcgac cgcggccggg
     1981 atcctggtca gcaaggcggc ggccgatacg gtcaagcgcg tctcgctgga actgggcggg
     2041 aaatcgccca acatcgtgtt cgacgatgcc gggctggacg cggcggtgac gcggggcgtg
     2101 cgccactgct tcaacaacac cggccagtcc tgcaacgcgc cgacccggat gctggtggag
     2161 caatcggcc
//
LOCUS       DASUDV010000549         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_284229,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000549 DASUDV010000000
VERSION     DASUDV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284229"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="VFV30_12060"
     CDS             complement(<1..84)
                     /locus_tag="VFV30_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein bL36"
                     /protein_id="HEX4848871.1"
                     /translation="MKIRNSLKSLKDRHRDNRVIRRRGRTYV"
     gene            complement(154..1161)
                     /gene="galE"
                     /locus_tag="VFV30_12065"
     CDS             complement(154..1161)
                     /gene="galE"
                     /locus_tag="VFV30_12065"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX4848872.1"
                     /translation="MTQKLPVLVTGGAGYIGSHAVLALQDAGWPVAVIDNLVTGFRWA
                     VPDGVPFYEGDIEDGALLARIFAEQGIGAVMHFAGSVVVPESVSDPLKYYHNNTAKTR
                     SLIASAVAGGVRHFIFSSTAATYGIPDVSPVREDTPQRPINPYGMSKLMTEFMLRDAA
                     AAHPLNFCALRYFNVAGADPQGRTGQSTAGATHLIKVAVEAALGKRERVDVFGTDFAT
                     PDGTGVRDYIHVSDLADAHVLALQALLDQPEHSLTMNCGYGRGYSVLEVLDAVDRVTN
                     QTIRRVLGPRRAGDPDSLISDNARIKATLPWVPKFADLDMIVAHALQWERKLSDLRPE
                     A"
     gene            1268..1861
                     /locus_tag="VFV30_12070"
     CDS             1268..1861
                     /locus_tag="VFV30_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX4848873.1"
                     /translation="MLRARLIPGLAAALLLAGCVTPVGPVQVTRFHAADVSPLGKGTI
                     AIEPAPGADGASLEWQTYRSAVMRQLEQIGYTEAGPGTAAQVAQVKLVRSTFQPGRGS
                     GPVSVGVGGSTGSYGSGVGVGVGLNLTPKPKPQVTTELAVAIRDRASGAVLWEGRASF
                     TVSTSSPLADTALAAPKVSAALFTNFPGTSGETIEVK"
     gene            1858..2994
                     /locus_tag="VFV30_12075"
     CDS             1858..2994
                     /locus_tag="VFV30_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEX4848874.1"
                     /translation="MTDIAIDAAFDSGNIEVLSIAGTTARLAIRKDQDSDFYQWFHFR
                     VSGAAGRALTLKITGLGGSAYPDGWPGYRAVVSEDRELWGRAETAYDKGEDGGTLTIR
                     YTPAANLAWFAYFAPYSMERHHDLVAGAALSDGVTHRVLGHSIDGQPIDCLDLGEGAK
                     QVWLYARQHPGESMAEWWMEGAIDRLTDPADPVARRLRQLCTFHVVPNANPDGSRRGH
                     LRTNAVGINLNREWDNPTPGKSPEVLAIRNAMDQTGVHFAIDVHGDEAIPAVFLAGFE
                     GIPSWSAELQGGFDRYKAILARRTPDFQTAKGYPVAGSGKANLTMSTNQLAERFGAVS
                     MTLEMPFKDNDDLPCAAQGWSPERSAQLGRDCLGALLEWLEDAG"
     gene            complement(2998..3495)
                     /locus_tag="VFV30_12080"
     CDS             complement(2998..3495)
                     /locus_tag="VFV30_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848875.1"
                     /translation="MKPNTRRALSVLLLGLAGWWTIDHQAEPATPDERAVLDLVVGRM
                     ADQCLAPATDDRPYSSSGFGTYGDPDGLALSLNYHLYASTGRDAVLLAKKDDEQSLLR
                     FVPTPLVPAGCTNATVLGTPTFMGKYAFVSYDRKREGGVFAFERGPAGWELVDSAHHR
                     AGPVI"
BASE COUNT          627 a         1223 c         1210 g          581 t
ORIGIN      
        1 cacataggtc cggccacggc ggcggatcac gcggttgtcc cggtggcggt ccttgagcga
       61 cttgaggctg ttgcgaatct tcatgggttt cctcggggcg gccgatgccg ctgaaatttg
      121 aaggtgcgcc gttaggggcg agtcagggcg aagtcaagct tcagggcgca ggtcggacag
      181 tttccgttcc cattgcagcg catgggccac gatcatgtcg agatccgcga atttcggcac
      241 ccacggcagg gtcgccttga tccgggcgtt gtccgaaatc agcgaatcgg gatcgccggc
      301 ccggcgcggg cccagcacgc ggcgaatcgt ctggttggtc acacggtcga ccgcgtcgag
      361 cacttcgagc accgaatagc cccggccata gccgcagttc atggtcagtg aatgctcggg
      421 ctggtccagc agcgcctgca gcgccagcac atgggcatcg gccagatcgc tcacatggat
      481 ataatcgcgc accccggtgc cgtcgggcgt ggcgaaatcg gtgccgaaca cgtcgaccct
      541 ttcgcgtttg cccagcgcgg cttcgaccgc gaccttgatc aggtgggtcg ccccggcggt
      601 cgattgcccg gtgcggccct gcggatcggc cccggccacg ttgaaatagc gcagcgcgca
      661 gaagttgagc ggatgggccg cggcggcatc gcgcagcatg aattcggtca tcagcttcga
      721 catgccatag ggattgatcg gccgctgcgg ggtgtcctcg cgcaccggtg agacatcggg
      781 aatgccatag gtcgccgcgg tgctgctaaa gatgaagtgc cgcaccccgc cggccaccgc
      841 gctggcgatc aggctgcggg tcttggcagt gttgttgtga tagtacttca gcgggtccga
      901 aaccgattcc ggcaccacca ccgatccggc gaaatgcatc accgcgccga tgccctgctc
      961 ggcaaagatc cgcgccagca gcgcgccgtc ctcgatatcg ccttcgtaga acggcacgcc
     1021 atcgggcacg gcccagcgga acccggtgac cagattgtcg atcaccgcca ccggccagcc
     1081 ggcatcctga agggcaagca cggcatggct gccgatgtat ccggcgccgc cggtaacgag
     1141 aacgggaagc ttttgagtca tggcccattt gccgctagca gatgccggca agcaagtggt
     1201 taacgaaacc gcaatccttg cgccgccatc ggcaaggggg caaactggtg ccagaggaga
     1261 ctgccgcatg cttcgcgccc gcctgatccc cggccttgcc gccgcgctgc tgctggccgg
     1321 ctgcgtcacg ccggtcggtc cggtgcaggt tacgcggttc cacgccgccg atgtctcgcc
     1381 gctgggcaag ggcaccatcg cgatcgaacc cgcgccgggc gccgatggcg cgtcgctgga
     1441 atggcagacc taccgttcgg cggtgatgcg ccagctggaa cagatcggct ataccgaagc
     1501 cgggccgggc accgccgcgc aggtggccca ggtcaagctg gtgcgcagca ccttccagcc
     1561 gggtcgtggc tctggcccgg tcagcgtcgg agtgggcgga tcgaccggca gctatggctc
     1621 cggggtcggc gtcggggttg gcctcaatct cacgcccaag cccaagccgc aggtcaccac
     1681 cgaacttgcc gtcgcgatcc gcgaccgggc gagcggcgcg gtgctgtggg aaggccgggc
     1741 cagcttcacc gtttccacgt cttcgccgct ggccgacacg gcgcttgccg cgcccaaggt
     1801 cagcgccgcc ctgttcacca atttcccggg cacttccggg gaaaccatcg aggttaaatg
     1861 accgacattg ccatcgacgc cgcctttgac agcggcaata tcgaagtcct gtccatcgcc
     1921 ggcaccaccg cgcggctggc gatccgcaag gaccaggatt cggatttcta ccagtggttc
     1981 cacttccgcg tctcgggcgc ggcggggcgc gcgctgacgc tcaagatcac cggcctgggc
     2041 ggctcggcct atcccgatgg ctggcccggc taccgcgccg tggtcagcga agaccgcgag
     2101 ctgtgggggc gggccgaaac cgcctatgac aagggcgagg acggcggcac cctgacgatc
     2161 cgctataccc ctgcggccaa tctcgcctgg ttcgcctatt tcgcgcccta ttcgatggaa
     2221 cggcaccacg atctggtggc cggcgcggcc ctgtcggacg gggtgaccca ccgggtgctg
     2281 ggccattcca tcgatggcca gccgatcgac tgcctcgacc tgggtgaggg cgccaagcag
     2341 gtctggctct atgcccgcca gcatcccggc gaatcgatgg ccgaatggtg gatggaaggg
     2401 gcgatcgacc ggctgaccga tccggccgat ccggttgcgc ggcggctgcg ccagctctgc
     2461 accttccacg tcgtgcccaa tgccaatccg gacggctcgc gccgcgggca ccttcggacc
     2521 aacgcggtcg gcatcaacct caaccgcgaa tgggacaacc cgaccccggg aaagtcgccc
     2581 gaagtgctgg cgatccggaa tgccatggac cagaccgggg tgcatttcgc gatcgacgtc
     2641 cacggcgacg aggcgatccc ggcggtgttc ctggccggct tcgaaggcat cccgtcgtgg
     2701 agcgccgagc tgcaaggcgg gttcgatcgc tacaaggcga tcctggcgcg ccgcaccccc
     2761 gatttccaga ccgccaaggg ctatccggtc gccgggtccg gcaaggccaa cctgacgatg
     2821 agcaccaacc agctggccga acggttcggc gcggtttcga tgacgctcga aatgcccttc
     2881 aaggacaacg acgacctgcc ctgcgcggcc cagggctgga gcccggaacg cagcgcgcag
     2941 ctcggccgcg attgcctggg ggcgctgctg gaatggctgg aggacgcggg ctgacggtca
     3001 gatgaccggg ccggcccggt ggtgcgccga atcgaccagc tcccacccgg cagggccgcg
     3061 ttcaaacgcg aagacgccgc cctcgcgttt ccggtcatag ctgacgaagg cgtatttccc
     3121 catgaaggtc ggcgtgccca gcacggtggc gttggtgcaa ccggcgggca ccaagggggt
     3181 cgggacaaag cgcagcagcg actgttcatc gtcctttttg gcgagaagga cggcatcgcg
     3241 gcccgttgag gcgtagagat ggtagttcag ggatagagca agaccgtccg ggtcgccgta
     3301 agtcccgaac cccgagctgc tgtaaggccg atcgtctgtc gcaggcgcga ggcactggtc
     3361 cgccatccgc cccacgacca gatccagcac ggcccgttcg tccggagtgg ccggttcggc
     3421 ctgatggtcg atggtccacc aaccggcgag gccaagcaac agcaccgaca gggctctgcg
     3481 tgtgttcggc ttcatccaaa aaagcccctt cacgttatgt aacaacataa cacgtcacag
     3541 cggagccgca accccttcag ccgtcaggcg accaccgcct ccagcacgcg caaggtccgc
     3601 gcatcggcat ccagctccac catcgcgccc gacggcaggg a
//
LOCUS       DASUDV010000550         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_947149,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000550 DASUDV010000000
VERSION     DASUDV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947149"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="rplA"
                     /locus_tag="VFV30_12085"
     CDS             <1..636
                     /gene="rplA"
                     /locus_tag="VFV30_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX4848876.1"
                     /translation="GVEAAIQLLKDLKTTKFDETLEVSLNLGVDPRHADQMVRGMVVL
                     PSGTGKDVKVAVFARGDNAQKALDAGADKVGAEDLLEDMQAGNLDYGRVIATPDMMGI
                     VGRLGKVLGPKGLMPNPKLGTVTPNVAEAVKAAKGGQIEFRVEKAGIIHGGIGKLSFS
                     NEALKANFDAFVDAVVKAKPAGAKGKYVRKVGLSSSMGPGLKIDLTEVQGA"
     gene            complement(717..1478)
                     /locus_tag="VFV30_12090"
     CDS             complement(717..1478)
                     /locus_tag="VFV30_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848877.1"
                     /translation="MAISFQRDTNFIERRRQGRAVLGIPVSVRERGRSALTARLADLT
                     SFGVRIDDMVLVQPGAQLWVKLPGLESLSVQLVWSSGASAGFVFDTPLHPAVAARFMP
                     AKGSHADAALTLPVHGHDPLLSRREQIMAGIAGSDHSPLQRKKKPSGMGLYGRINRTV
                     SRQADTRAERRYGDAVPEGTALSIGGTPAEVLNVSPSGLKVRCDMGERDIGTELGIAF
                     DGFEPMTGQLVWINGPEAGIALPPQSIELFDRAAG"
     gene            1815..2438
                     /locus_tag="VFV30_12095"
     CDS             1815..2438
                     /locus_tag="VFV30_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848878.1"
                     /translation="MLRFALPLVALALGACSGGKPDAPATDVALVPSETAPAEAATPV
                     PDAAAAVMARTSQYTSLKDCKVIDDGGGEDWSVSRCAGLGGITLMINYGDARDDLELH
                     LPGKQPTELGLPYLAGGGFNTLGDTVEWRGTGQGKAFRPSALIVRNHAVQDPEHPERP
                     TALLAVIDLTKGCVVAQIKPQAGQNEAARALADGPPRPCLDPGRAVG"
     gene            2435..2812
                     /locus_tag="VFV30_12100"
     CDS             2435..2812
                     /locus_tag="VFV30_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4848879.1"
                     /translation="MIRTLMLLAATTAAVAPGASAPETHADFDVARGKPQRVIVQSRD
                     FASGAESGWHVHPGVEVAYISQGELELHVKGKVTVLKAGDSFTMPRGVPHNGVNRSAG
                     TARAVITLVVDKGAQPRQPVPAP"
     gene            complement(2809..>3034)
                     /locus_tag="VFV30_12105"
     CDS             complement(2809..>3034)
                     /locus_tag="VFV30_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848880.1"
                     /translation="AEMNRQLAETQVPFGRVAASLNFSREPLASARRGDPACPAGAIS
                     THRALLRLPDGRPLAFVVECYTEANLAPAT"
BASE COUNT          496 a         1023 c         1067 g          448 t
ORIGIN      
        1 ggcgtcgaag ccgcgatcca gctgctcaag gacctcaaga ccaccaagtt cgacgaaacc
       61 ctggaagttt cgctgaacct gggcgtcgat ccgcgccacg ccgaccagat ggtccgcggc
      121 atggtcgtgc tgccctcggg caccggcaag gacgtcaagg ttgcggtctt cgcccgcggt
      181 gacaacgccc agaaggcgct ggacgccggt gccgacaagg tgggcgccga agacctgctg
      241 gaagacatgc aggccggcaa cctcgattac ggccgcgtga tcgccacgcc cgacatgatg
      301 ggcatcgtcg gccgcctcgg caaggtgctg ggtcccaagg gcctgatgcc gaacccgaag
      361 ctgggcaccg tcaccccgaa cgtcgccgaa gcggtcaagg ctgccaaggg cggccagatc
      421 gaattccgcg tcgaaaaggc cgggatcatc cacggcggga tcggcaagct gtcgttctcg
      481 aacgaagcgc tgaaggccaa cttcgacgct ttcgtcgatg cggtggtcaa ggccaagccg
      541 gccggcgcca agggcaagta cgtccgcaag gtcggcctgt cctcgtcgat gggcccgggc
      601 ctgaagatcg acctgaccga agtgcagggc gcctaagccc tgccttcgct ggcatgaaaa
      661 agggccggga agctaagcgc ttcccggccc ttttcgtttg tgccgcgccg tgcgggttaa
      721 cccgccgcgc ggtcgaacag ttcgatcgat tgcgggggca gggcgatccc ggcctcgggc
      781 ccgttgatcc agaccagctg gccggtcatc ggctcgaacc cgtcaaaggc gatgcccagt
      841 tccgtgccga tatcgcgctc gcccatgtcg cagcgcacct tgaggccgct gggcgagacg
      901 ttgagcactt cggccggggt ccccccgatc gacagcgcgg tgccttcggg cacggcatca
      961 ccataacggc gctcggcccg ggtatcggcc tggcggctga ccgtgcggtt gatccgcccg
     1021 taaaggccca tcccggacgg cttcttcttg cgctgcagcg gggaatggtc cgatccggcg
     1081 atcccggcca tgatctgttc gcgccgcgac agcagcggat cgtgtccgtg gaccggcagg
     1141 gtcagcgcgg catcggcatg gctgcccttg gccggcatga accgcgccgc gaccgccggg
     1201 tgcagcgggg tgtcgaacac gaagccggcc gaggcgccgc tcgaccagac gagctggacg
     1261 gagaggcttt ccagccccgg cagcttcacc cacagctgcg cgccgggctg gaccagcacc
     1321 atgtcgtcga tccgcacgcc gaacgaggtc aggtccgcca gccgcgcggt cagggccgag
     1381 cgtccgcgct cgcgcaccga gacgggaatg cccagcacgg cacggccttg ccgccgccgt
     1441 tcaatgaaat tggtatcgcg ctgaaaactg atagccacgg ggtcacactc cgacatagga
     1501 cttgcgttac gttaggtgcg aacccggctt tgcgtcttgc cttgcccttg cgatggcacg
     1561 ttgcgggggc cgcgcaaccg ggtccccggc ggcgtcccgg gctgggatta acgcgcatgg
     1621 ttaagttaaa cttaccgccc ggccgcaggc gcggagaaaa cgccgtgatg ccagagactt
     1681 gcctgccggc cagttgaaaa ccgtggcgat tctgccggat tcagcgccct ggtgaggcgg
     1741 agaagggggc ttggggaccc cgcgcgaagg gcccgcctat cgccgcgaat ccggctgtgg
     1801 caggatcgcc ggcgatgctg cgattcgccc tgcccctggt ggccctcgcc ctcggcgcct
     1861 gttccggcgg caagccggac gcgcccgcaa ccgatgtggc cctggtgccg tcggaaacgg
     1921 cacccgcgga agcggccacc cccgttcccg atgcagcggc cgccgtgatg gcgcgcacct
     1981 cgcagtacac ctcgctcaag gactgcaagg tgatcgacga tggcggggga gaggactggt
     2041 cggtttcgcg ctgcgctggc ctgggtggga tcacgctgat gatcaattac ggcgatgccc
     2101 gcgatgatct ggagctgcac ctgccaggca agcagccgac cgagctgggg ctgccctatc
     2161 tggccggcgg cgggttcaac accctgggcg atacggtgga atggcgcggc acggggcagg
     2221 gcaaggcctt tcgcccctcg gcgctgatcg tgcgcaacca tgcggtgcag gatcccgaac
     2281 accccgaacg ccccaccgcg ctgctggcgg tgatcgatct gaccaagggc tgcgtcgtgg
     2341 cccagatcaa accgcaagcc gggcagaacg aggccgcccg ggcccttgcc gacgggccgc
     2401 cgcgcccctg cctcgatccc ggaagggccg tgggatgatc cgcaccctga tgctgctcgc
     2461 tgccaccacg gcggccgtcg cgcccggtgc ctcggcgccg gagacccatg ccgacttcga
     2521 cgtggcgcgg ggcaagccgc aacgggtcat cgtccagagc cgcgattttg cttccggtgc
     2581 ggaaagcggc tggcacgtcc atcccggggt cgaagtggcc tatatcagcc agggcgagct
     2641 ggagctgcac gtcaagggca aggtcaccgt gctgaaggcc ggggacagct tcaccatgcc
     2701 gcgcggggtg ccgcacaacg gggtcaaccg cagtgcgggc acggcccggg cggtgattac
     2761 cctggtggtg gacaagggcg cgcagccgcg ccagccggtg cccgcgcctt aggtggcggg
     2821 ggccaggttg gcttcggtgt agcattccac cacgaaggcc agcggccgcc catcgggcag
     2881 gcgcagcagc gcgcggtggg tggaaatggc gccggccggg caggccggat cgccgcgccg
     2941 ggccgatgcc agcggttccc gcgagaagtt gagcgaggcg gcaacccggc cgaacggcac
     3001 ctgggtttcg gccagttggc ggttcatctc cgcg
//
LOCUS       DASUDV010000551         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_85743,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000551 DASUDV010000000
VERSION     DASUDV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85743"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFV30_12110"
     CDS             complement(<1..501)
                     /locus_tag="VFV30_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX4848881.1"
                     /translation="MRVSIFPLPGAILFPGLQLPLHIFEPRYRALVKDALARDRRIGM
                     IQPQRAEEGAPLFRIGCLGKIGEVEALDDGRFNLVLEGEARFRIVRELDAATPFRQVE
                     AELLDEEDYPALSPVERASFEREARRFADAQGYSVDWEAVTRLDDQSLIDGTSQIVPF
                     DIASKQA"
     gene            complement(505..1398)
                     /locus_tag="VFV30_12115"
     CDS             complement(505..1398)
                     /locus_tag="VFV30_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4848882.1"
                     /translation="MGLNIEEQKAVDRFRKNVVEPSMDSLVILDFWAEWCGPCKALTP
                     VLEKVAADYADKGVVLAKVNVDEEGFIASQFQVRSIPTVYAIFQGQPVADLTQARSES
                     QLKGILDQLLAKLPVKAGGAPAQDVAPLLAMGEEVLAGGEADRAAAIFSQIIEIDPGS
                     AAAYGGLIRAVTAMGEAAQADEMLAALPPELAGDAEIARARAALELAKNRPEDSELAA
                     LRSAATADNPAGQLAYAEAAFAAGDRDSAADTLLGMIAADREWNEGAAKAKLLQIFEA
                     VGLEDAWVAAQRRRLSLILFG"
     gene            1578..1652
                     /locus_tag="VFV30_12120"
     tRNA            1578..1652
                     /locus_tag="VFV30_12120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1610..1612,aa:Gly,seq:gcc)
     gene            1759..2316
                     /locus_tag="VFV30_12125"
     CDS             1759..2316
                     /locus_tag="VFV30_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848883.1"
                     /translation="MTGFLLALIATVLVGFGARDQVLVAGFSARQGQRPALLLVGLLS
                     AAVAAGGALWLAGAFAAGLAAPVRTMLAVLALGLAGIEMLLTRPGPRPAEPTESLGAF
                     ALVLLAGQLTDAARFLLMALSLASVLPGAAGLGGALGGMAVVAIGWAGGQALLDLPLV
                     RIRRWLGGALLIAAAVLVAVFRQAG"
     gene            2396..3016
                     /locus_tag="VFV30_12130"
     CDS             2396..3016
                     /locus_tag="VFV30_12130"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX4848884.1"
                     /translation="MTSATLIADADDGAIALWLHSRLDAALAAAPGPIAITIPGGSTP
                     FPILAELARLGLDWSRIAVWPGDDRIVPEDHPASNAGRIRAALEPLGAQVVALEEDAV
                     PPHFALAWLGMGTDGHIASLFPNTDPRTDDPQPVRRLVPDPLPPEAPFARLSLTLPAL
                     LDSDALVFVIRGAEKRALFEAALRGEHDLPVARLLAAAHQPVTCFT"
BASE COUNT          404 a         1096 c         1114 g          510 t
ORIGIN      
        1 ggcctgcttc gaggcgatgt cgaacggcac gatctggctg gtgccatcga tcagcgactg
       61 atcgtccagc cgggtcacgg cttcccaatc gaccgaatag ccttgcgcat cggcaaacct
      121 gcgggcctcg cgctcgaacg aagcgcgctc gaccggagac agggcgggat agtcttcctc
      181 gtccagcagt tcggcctcga cctgcctgaa cggcgtggcg gcatcgagct cgcggacgat
      241 gcggaaccga gcttcgccct ccagcaccag gttgaaacgg ccgtcgtcga gcgcctcgac
      301 ctcgccgatc ttgccaaggc agccgatccg gaacagcggc gcgccttctt cggcgcgctg
      361 cggctggatc atgccgatcc ggcggtcgcg tgccagcgca tccttcacca gcgcccggta
      421 gcgcggctcg aagatgtgca gcggcaactg cagccccggg aacaggatcg ctccgggcag
      481 gggaaagatc gaaacgcgca tgacctagcc gaacaggatc aaggaaaggc ggcggcgctg
      541 ggcggcaacc caggcatcct ccagcccgac cgcttcgaag atctgcagca gcttggcctt
      601 ggccgcgccc tcgttccatt cgcggtcggc ggcgatcatg cccagcaggg tatcggccgc
      661 gctgtcgcgg tcgccggcgg caaaggcggc ttcggcatag gccagctgcc cggccgggtt
      721 gtcggccgtg gcggcgctgc gcagcgcggc cagttcgctg tcctcgggcc ggttcttggc
      781 cagttccagc gcggcgcggg cgcgggcaat ttcggcatcg ccagcgagtt cgggcggcag
      841 tgcggccagc atctcgtcgg cctgggcggc ttcgcccatt gcggtcacgg cgcggatcag
      901 cccgccatag gccgcggcgc tgcccggatc gatttcgatg atctgcgaaa agattgctgc
      961 cgcccggtcg gcctcgccgc cggccagcac ctcttcgccc atcgccagca gcggggcgac
     1021 atcctgagcc ggggccccgc ccgccttgac cggcagcttg gcgagcagct ggtcgagaat
     1081 gcccttgagc tgcgattcgc tgcgggcctg ggtcagatcg gccaccggct ggccctgaaa
     1141 aatggcatag accgtgggga tcgagcggac ctggaactgg ctggcgatga agccttcctc
     1201 gtcgacattg accttggcga ggaccacgcc cttgtcggca tagtcggccg cgaccttttc
     1261 cagcaccggg gtcagcgcct tgcacggacc gcaccattcg gcccagaaat ccaggatcac
     1321 gagcgaatcc atcgagggct cgaccacgtt cttgcggaac cggtccaccg ccttctgttc
     1381 ctcgatgttc agccccatgc tcgccacgtc aaactcctgc aaaacaaatc gctcggcgcc
     1441 aatgtgggcc tttgcgccca tttgtgaagg gatatttgcc gcattcgcgt ttgcggcaat
     1501 tttccccttg gcaagggcaa ggggcggtgc taaccgcgcg cctcacccgg acggcctgcc
     1561 cgtcccatgg cgccagagcg ggcgtagctc aggggtagag cacaaccttg ccaaggttgg
     1621 ggtcgagggt tcgaatccct tcgcccgctc cagtcttctc agacatttac ggttccggat
     1681 ggcgcagccc ccgcgctggg ccgccgcttt gcgcgcgcgg cgcttgaccg cgccgcctgg
     1741 cccggtgcag cagggggcat gaccgggttt ctccttgccc tgatcgccac cgtgctggtc
     1801 ggcttcggcg cgcgcgacca ggtgctggtg gcgggctttt cggcgcgcca gggccagcgt
     1861 ccggcgctgc tgctggtcgg cttgctcagc gccgcggttg ccgcgggcgg ggcgctgtgg
     1921 ctggccgggg cctttgctgc ggggctggcc gcgccggtgc ggaccatgct ggcggtgctg
     1981 gccctggggc tggccgggat cgagatgctg ctgacccgcc ccgggccgcg gcccgccgaa
     2041 ccgaccgaat cgctgggcgc cttcgcgctg gtcttgctgg ccgggcagct gaccgacgcc
     2101 gcgcggttcc tgctgatggc gctgagcctc gcctcggtcc tgccgggcgc ggcggggctg
     2161 ggcggggcgc tgggcgggat ggcggtggtg gcgatcggct gggccggggg gcaggcgctg
     2221 ctggacctgc cgctggtgcg gatccggcgc tggctgggcg gggccctgct gatcgcggcg
     2281 gcggtgctgg tcgcggtgtt ccgccaggcg ggctgaacgg atgccccggt ccggccccgc
     2341 gcatagcatt gcagccccgt tccgccaccg ggccgcgcct gctatcgcag acggcatgac
     2401 ctctgccacc cttatcgccg acgccgacga cggcgccatt gccctgtggc tgcacagccg
     2461 gctggacgcc gcgctggccg ctgcgcccgg cccaatcgcg atcaccatcc cgggcggatc
     2521 gaccccattc ccgattctgg ccgagctggc ccggctgggg ctggactgga gccggatcgc
     2581 ggtctggccc ggggatgacc ggatcgtgcc cgaggatcac ccggccagca acgccgggcg
     2641 gattcgcgcc gcgctggagc cgctcggcgc gcaggtcgtg gcgctggaag aggacgccgt
     2701 gccgccgcat ttcgcactgg cctggttggg catgggcacc gacggccata tcgccagcct
     2761 gtttccgaac accgatcccc ggaccgacga tccgcagccg gtgcggcggc tggtgcctga
     2821 tcccctgccg cccgaagcgc cctttgcccg gctcagcctg accctaccgg ccttgctgga
     2881 cagcgacgcg ctggtgttcg tgatccgcgg ggcggagaag cgggcgctgt tcgaagcggc
     2941 gctgcgcggc gagcacgacc tgccggtcgc gcggctgctg gcggcggcgc atcagccggt
     3001 cacatgcttc acctgatccc ggccccgctg caccgggccg ggctgcggct ggcccatgcc
     3061 gtgcggcggc gctggtggcg gctggccggc atcaagctgc gcggctgccg ggtcctggcc
     3121 ttcg
//
LOCUS       DASUDV010000552         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_41774,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000552 DASUDV010000000
VERSION     DASUDV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41774"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1245)
                     /locus_tag="VFV30_12135"
     CDS             complement(148..1245)
                     /locus_tag="VFV30_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEX4848885.1"
                     /translation="MAESYEFIDINARGDGIVAAAFDNQVAYCTASGADRTARICAAV
                     RDLLARGEPGLLLDRLRAWPGAPLADALPLRLAGGLHSLHLSGAEPALGAIYAGEAGI
                     DDAAIVGAAIRQHEAELLPWLDGPPQTNEAGRSSNFLAAMLWLADKGLPPRFECLEIG
                     SSAGINLMLDRYHYDLGGVQVGPEPGAMRFAPEWQGDAPPAHPIEIVGAKGCDVAPVD
                     LTDPAQALRLKAYIWPEHTVRFERMEAAIAEAAKSPPALVHQNAADFIEAELARPQQA
                     GTTRMLMHSIVWQYVPDDQKARVTAAMEAAGAKATAERPLAWVALEANRVMHVHEMVV
                     RYWPGGDTGEIVTRAHPHGAWIAWGGSERTI"
     gene            1370..2029
                     /gene="hisG"
                     /locus_tag="VFV30_12140"
     CDS             1370..2029
                     /gene="hisG"
                     /locus_tag="VFV30_12140"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX4848886.1"
                     /translation="MANALTFAVPKGRILDEALPMMARAGVVPEAGFHDKGNRALSFA
                     CEGSDMRIIRVRAFDVATFVAHGAAQLGIVGSDVVEEFAYPDLYAPVDLDIGHCRLSV
                     AEPEGQAAAPGGHVSHLRVASKYPSLTRRHFEQLGVQAEVVKLNGAMELAPGLGLASR
                     IVDLVSTGRTLKDNGLVETSRILDVSARLIVNRAALKTDERVAALVEQFRALVAAAKA
                     A"
     gene            2029..3321
                     /gene="hisD"
                     /locus_tag="VFV30_12145"
     CDS             2029..3321
                     /gene="hisD"
                     /locus_tag="VFV30_12145"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX4848887.1"
                     /translation="MLRLNTRDPGFDKAFRRLVADRRESGDDVSRDVQVILADVRNRG
                     DAALADLTARFDNHRLVEDADWRVSAEACHEAYEALKPDLRAALELAAQRIRAFHEKQ
                     RPKNRDETDEVGVRMGAVWRPVDAAGLYVPGGRAAYPSSLLMNAIPAKVAGVERLVVV
                     TPSPKGEMNPLVLAAAHLAGVDEVWRIGGAQAVAALAYGTQRIAPVDVITGPGNAWVA
                     EAKRQLYGVVGIDMVAGPSEILLIADGQNDPEWTAADLLSQAEHDPAAQSILITDDPL
                     FADVVADRIGVELAMLPTARVANESWNTHGVIIEVASLDEAPALANALAAEHVQIATD
                     QPEKLFKAIRHAGSVFLGRMTPEAVGDYVAGPNHVLPTGRRARFSSGLSVLDFMKRTS
                     FIQLDEAALKAVGPAAIELADAEGLTAHARSIEVRLGI"
     gene            3318..3764
                     /gene="nusB"
                     /locus_tag="VFV30_12150"
     CDS             3318..3764
                     /gene="nusB"
                     /locus_tag="VFV30_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX4848888.1"
                     /translation="MKAGSKARSAARLAAVQALYQLEMEATPMASLLDEFHRHRLGAE
                     IEGDQYAEAEVPFFDAIVQGVSARRDEIDDVLAGRLAEGWKLERLDRTMHQILRAGAW
                     ELMARADVPTATVISEYLDVAHAFFEAREAKFVNGVLDGVAKGVRS"
BASE COUNT          594 a         1353 c         1271 g          610 t
ORIGIN      
        1 agcttcacca ccctgcaggt cggcgggatg gccatcgcga tcttcggcac cgtcgccggg
       61 tgggaatggg tgtcctacca cggcggctgg aaggaacgga tcgacgatct ggccgcctgg
      121 ttccgcctcg cccgcagcta ctgaccgtca gatcgtccgc tcgctgccgc cccaggcgat
      181 ccacgcgccg tgcggatggg cgcgcgtaac gatctcgccc gtgtccccgc cgggccagta
      241 ccgcaccacc atttcgtgga cgtgcatcac ccggttcgct tccagcgcaa cccaggccag
      301 cggccgctcc gccgtggcct ttgctccggc cgcctccatc gcggcagtaa cccgcgcctt
      361 ctgatcgtcc ggcacatatt gccagacgat cgaatgcatc agcatccggg tggttccggc
      421 ttgctgcggc cgcgccagtt cggcctcaat gaaatcggcg gcgttctggt ggaccagagc
      481 aggcggagac ttcgccgcct cggcaatcgc cgcttccatc cgctcgaacc gcacggtgtg
      541 ttcgggccag atgtaggcct tcaggcgcag cgcctgggcc ggatcggtca gatcgaccgg
      601 cgcgacgtcg cagcccttcg cgccgacgat ttcgatcgga tgcgcgggcg gagcgtcgcc
      661 ctgccattcg ggggcaaagc gcattgcgcc cggttccggc cccacctgta ccccgcccag
      721 atcgtagtga tagcgatcga gcatcaggtt gatcccggcg ctcgatccga tttccaggca
      781 ttcgaaccgc ggcggcaggc ccttgtccgc cagccacagc atcgcggcga ggaaattgct
      841 ggaacggccc gcctcgttgg tctgcggcgg gccatcgagc cagggcaaca actcggcctc
      901 gtgctggcgg atcgcggccc cgacgatcgc ggcgtcgtcg atccccgctt cgccggcata
      961 gatcgcgcca agcgccggtt ccgcgccgct caaatgcaga gagtggagcc ccccggccag
     1021 ccgcagcggc agggcatcgg ccagcggcgc gcccggccag gcgcgcagtc ggtccagcag
     1081 cagccccggc tcgccccgag ccagcaggtc ccgcacggcg gcacagatcc gcgccgtccg
     1141 gtccgcgccc gaggccgtgc agtaggccac ctggttgtcg aatgcggccg cgacgatccc
     1201 gtcgccgcgc gcgttgatgt cgatgaattc gtaggattcc gccatcgccg ttctcccctg
     1261 cgcacttttg tgcatctgcg aacggattgc gcctttccgc tgcatcgcac aaggaaaatc
     1321 cgccccgctc cttgcccttt cgcgcctcgc accgtaaggg ccgcgggcta tggcaaacgc
     1381 gctgaccttc gccgttccca agggccgcat cctcgatgaa gcgctgccga tgatggcgcg
     1441 tgccggggtc gttccggaag ccggttttca cgacaagggc aaccgcgccc tctcgttcgc
     1501 ctgtgaaggc tcggacatgc ggatcatccg cgtgcgggct ttcgatgtcg ccacctttgt
     1561 cgcccacggc gcggcgcagc tggggatcgt ggggtccgac gttgtggagg aattcgccta
     1621 tccggatctc tatgccccgg tcgatctcga catcggccat tgccggctgt cggtggcaga
     1681 gcccgaaggc caggccgccg cgccgggcgg gcacgtcagc cacctgcgcg tcgccagcaa
     1741 gtatcccagc ctgacccgcc gccatttcga acagctgggg gtccaggccg aagtcgtgaa
     1801 actgaacggc gcgatggaac tggcccccgg gctgggcctg gccagccgga tcgtcgatct
     1861 tgtctccacg ggccgcacgc tcaaagacaa cggcctggtt gagaccagcc ggatcctcga
     1921 tgtctcggcc cgcctgatcg tcaaccgcgc cgcgctcaag accgacgagc gggttgccgc
     1981 tctggtcgaa cagttccgcg cccttgtggc cgcagcgaaa gccgcctgat gctccgtctc
     2041 aacacccgcg atcccggctt tgacaaggcg ttccggcggc tggttgccga ccggcgcgaa
     2101 tccggcgacg atgtctcgcg cgatgtccag gtgatcctgg ccgatgtgcg caaccgcggc
     2161 gacgccgcgc tggccgatct gaccgcccgg ttcgacaacc accggctggt cgaggacgcc
     2221 gattggcgcg tttcggccga ggcctgccac gaggcttacg aagcgctgaa gcccgatctg
     2281 cgcgccgcgc tggaactggc cgcgcagcgg atccgcgcct tccacgaaaa gcagcgcccc
     2341 aagaaccgcg acgagaccga cgaggtgggc gtgcggatgg gcgcggtgtg gcggccggtc
     2401 gatgcggccg ggctttacgt gccgggcggg cgcgcggcct atcccagctc gctgctgatg
     2461 aacgcgattc ccgccaaggt ggccggggtg gagcggctgg ttgtcgtcac ccccagcccc
     2521 aagggcgaga tgaacccgct ggtgctggcc gccgcgcatc tggccggcgt ggacgaggtc
     2581 tggcggatcg gcggggcgca ggccgtggcc gcgctggcct atggcacgca gcggatcgcc
     2641 ccggtcgatg tcatcaccgg ccccggcaac gcctgggtgg ccgaggccaa gcgccagctt
     2701 tacggcgtgg tcgggatcga catggtcgcc ggccccagtg aaatcctgct gatcgccgat
     2761 ggccagaacg atcccgaatg gaccgcggcc gatctgctga gccaggccga gcacgatccc
     2821 gccgcgcaat cgatcctgat caccgacgat ccgctgttcg ccgatgtggt ggctgaccgg
     2881 atcggggtgg aactggccat gctgccgacc gcgcgggtcg ccaacgaaag ctggaacacc
     2941 cacggcgtga tcatcgaggt cgccagcctg gacgaagccc cggcgctggc caacgcgctg
     3001 gccgcggaac atgtccagat cgccaccgac cagccggaaa agctgttcaa ggcaatccgc
     3061 catgccggct cggtcttcct gggccggatg accccggaag cggtgggtga ctatgtcgcc
     3121 ggcccgaacc acgttttgcc caccgggcgg cgcgcgcggt tctcttcggg cctctcggtg
     3181 ctcgatttca tgaagcggac cagcttcatc cagcttgacg aagcggcgct gaaggccgtc
     3241 ggtccggccg cgatcgaact ggccgatgcc gaggggctga ccgctcacgc ccgctcgatc
     3301 gaagtaaggc taggaatatg aaggccggat caaaggctcg ctccgccgca cggcttgccg
     3361 ccgtgcaggc gctctaccag ctcgaaatgg aagcgacgcc gatggcttcg ctgctcgacg
     3421 agtttcaccg ccatcggctt ggcgccgaaa tcgaaggcga tcaatacgcc gaagccgagg
     3481 tgccgttctt cgatgcgatc gtgcagggcg tcagtgcccg ccgcgacgag atcgacgacg
     3541 tcctggccgg ccgcctcgcc gaaggctgga agctggaacg gctcgaccgc accatgcacc
     3601 agatcctgcg cgccggcgcc tgggaactga tggcccgggc cgacgtcccg accgccacgg
     3661 tcatcagcga atatctcgac gtcgcccacg cgttcttcga agcgcgcgaa gccaagttcg
     3721 tgaacggcgt gctcgacggc gttgccaagg gcgtccgtag ctgagcggcg aagcctcatt
     3781 catcgccgcg ctgcgccgcc tggcgcagca tcccgccgcg cgcggcct
//
LOCUS       DASUDV010000553         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1102097,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000553 DASUDV010000000
VERSION     DASUDV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102097"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /gene="astD"
                     /locus_tag="VFV30_12155"
     CDS             <1..1388
                     /gene="astD"
                     /locus_tag="VFV30_12155"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEX4848889.1"
                     /translation="PATGAELWRGKHGNVDDIVGRARRAWPAWAAQPLANRMELVRRF
                     ANEVRKESEKLATLIARETGKPLWEARTEVESVINKVEISIRAYAERTAQRKLDNALQ
                     GTAAVRHKPHGVMAVLGPYNFPAHLPNGHIIPALIAGNAIVFKPSEKTPATAELLVQC
                     LHKAGISAAVVQLLIGGPEEGQALVGHDGIDGVLFTGSAHTGIAINRRLATRPDKIVA
                     LEMGGNNPLVVWDTPKLTDAAALVIQSAFTSSGQRCTAARRLIVKSTMYDALIAEVKR
                     LADRIIVGGPFDEPSPFMGPVIDNQAADGLTESFLYLLSHGGKAIKHLVRAREDLPFL
                     SPAIIDVTGVKDRPDVELFGPLLQVFRVDDFDEAIAEANNTRFGLSASLVGGTPAEYN
                     RFWANVRAGVINWNRPTNGASSAAPFGGVGLSGNHRPSAYYAADYCAYPVASNEMEQP
                     RAMIGVGLREE"
     gene            complement(1396..2106)
                     /locus_tag="VFV30_12160"
     CDS             complement(1396..2106)
                     /locus_tag="VFV30_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848890.1"
                     /translation="MRRSILLLPLMLTLAACGGDAPADGRENRDPAVSAALDDPLMAD
                     PDLAAQNRGNSALSGGGPATAEVPPDQRSPEAAARGREAAAQLLGGRIDPAPGPSATL
                     PESPLKGALTLEAIAAALKLGGADCPAKVGYGFAWAARLPTALPVYPRGHTRVAAGSD
                     EGACRLRIVKFVTAVPAGEVIDFYFAAARKAGLNPEVRREGEDLVVIGGKGAARFAVY
                     ARAAGDGLSEVDLATAGL"
     gene            2271..3191
                     /gene="rarD"
                     /locus_tag="VFV30_12165"
     CDS             2271..3191
                     /gene="rarD"
                     /locus_tag="VFV30_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX4848891.1"
                     /translation="MAPPPHSENSKGLAAALATYLLWGIMPLYLILVKAVPPIEFVGW
                     RIVFTVPVCLLLVAALRQGAALRAALDWPVLRMLMLSAFLVGINWLVYTVAVQTGHVI
                     ATSIGYYMNPLANVLFGTLFLKERLSTRQWLAVGLAAVGVSLLAWDALDTMGISLALA
                     ISFSAYGVVRKLAPVDSLPGLTVETGVLLIPAALLVGWFAASPAGSAFGADPALSGLI
                     AISGVVTAVPLLMFAYAAKRMDYSALGMVQFFSPTIVFFLGLFVFGEPLKPSQLACFL
                     VIWTAIAVFMWDLYDQRRKRLAQLAQEAPA"
     gene            complement(3168..>3456)
                     /locus_tag="VFV30_12170"
     CDS             complement(3168..>3456)
                     /locus_tag="VFV30_12170"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980056.1"
                     /note="catalyzes the formation of N-carbamoyl-L-aspartate
                     from (S)-dihydroorotate in pyrimidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4848892.1"
                     /translation="PHAVGAKESSCGCAGIFNAPFALESYIAVFEEEGALDRFEGFAS
                     LHGPAFYGLPVNAETVTLEKAPVTVPDLIDANGTPIVPFHAGETLGWRFLG"
BASE COUNT          524 a         1236 c         1168 g          528 t
ORIGIN      
        1 aaccggctac cggcgccgaa ctgtggcgcg gcaagcacgg caatgttgac gacatcgttg
       61 gacgggcccg ccgggcctgg ccggcctggg cggcgcagcc gcttgccaac cggatggagc
      121 tggtccgccg cttcgccaac gaagtgcgca aggaaagcga aaagctcgcc acgctgatcg
      181 cccgcgaaac cggcaagccg ctgtgggaag cgcggaccga agtcgaaagc gtcatcaaca
      241 aggtcgaaat ctcgatccgc gcctatgccg agcggaccgc ccagcgcaag ctggacaacg
      301 cgctgcaggg caccgccgcg gtgcgccaca agccgcacgg cgtgatggcg gtgctgggcc
      361 cctacaattt ccccgcgcac ctgcccaacg gccacatcat tccggcgctg atcgcgggca
      421 acgcgattgt cttcaagcct tcggaaaaga ccccggcaac ggccgagctg ctggtccagt
      481 gcctgcacaa ggccggcatt tccgccgccg tggtccagct gctgatcggc ggcccggaag
      541 agggccaggc gctggtcggg catgacggga tcgacggggt gctgttcacc ggatcggccc
      601 acaccgggat cgcgatcaac cgccgcctcg ccacccggcc ggacaagatc gtcgcgctgg
      661 aaatgggcgg caacaacccg ctggtggtgt gggacacgcc caagctgacc gatgcggccg
      721 cgctggtgat ccagtcggcc tttacctcgt ccgggcagcg ctgcaccgcg gcgcggcggc
      781 tgatcgtgaa aagcacgatg tacgacgcgc tgatcgcgga agtgaagcgg ctggccgacc
      841 ggatcatcgt gggcggcccg tttgacgagc ccagcccctt catgggcccg gtgatcgaca
      901 accaggctgc cgacggcctg accgaaagct tcctctacct cctcagccac ggcggcaagg
      961 cgatcaagca cctggtccgc gcgcgcgagg atctgccgtt cctctcgccc gcgatcatcg
     1021 acgtgaccgg ggtgaaggac cgccccgacg tggagctgtt cggcccgctg ctgcaggtct
     1081 tccgggtcga cgatttcgac gaggcgattg ccgaggcgaa caacacccgc ttcggcctct
     1141 cggcctcgct ggtgggcggc acgccggccg aatacaaccg cttctgggcc aatgtccgcg
     1201 cgggggtgat caactggaac cgtccgacca acggggccag ctcggccgcg ccgttcggcg
     1261 gggtgggcct gtcgggcaac caccgcccca gcgcctatta cgcggcggac tattgcgcct
     1321 atccggtggc ctccaacgaa atggaacagc cccgggcgat gatcggcgtc ggcctgcgcg
     1381 aggaatagcg cggggtcaca gcccggcggt tgccaggtcc acttcgctga gcccatcgcc
     1441 ggccgcgcgg gcatagaccg caaagcgcgc cgcgcccttg ccgccgatca cgaccagatc
     1501 ctcgccctcg cgccggacct cgggattgag cccggccttg cgcgcggcgg caaagtagaa
     1561 atcgatcact tcgccggccg gcaccgcggt gacgaacttg acgatccgca gccggcaggc
     1621 gccttcgtcg ctgcccgcgg caacgcgcgt gtggccgcgc ggatagaccg gcagcgccgt
     1681 cggcagccgc gccgcccagg caaagccgta gcccaccttg gccgggcagt ccgccccgcc
     1741 cagcttcagc gcagcagcaa tcgcctccag cgtgagcgcg cctttcagcg gcgattcggg
     1801 cagcgtcgcg gacggccccg gggccggatc gatccgcccg ccgagcagct gcgctgccgc
     1861 ctcgcgcccg cgcgccgccg cttcggggct gcgctggtcc ggcggcactt cggccgtcgc
     1921 cggcccgccg cccgacaggg cggaattgcc gcggttctgt gccgccagat cgggatcggc
     1981 catcagcgga tcgtccagcg cggccgagac cgccggatcg cggttctcgc gcccgtcggc
     2041 cggggcatcg ccgccgcagg cggccagggt cagcatcagg ggcagcagca ggatcgaacg
     2101 gcgcacaggg ctgggtctcc gccaggggaa gccccgtcat gccgcatcgc gcggcgcaat
     2161 gccagatgga cccgcgccaa aagaaccgcc gcgaatgctt cccaacaatt gcgcaaaggg
     2221 ccccgccccg cgcttgccac tgccgcgccc ccccgctagc gcccatcgcc atggccccgc
     2281 ccccgcactc cgagaattcc aaaggcctgg cggcggcgct tgccacctac ctgctgtggg
     2341 gcatcatgcc cctctacctg atcctggtga aggcggtgcc gccgatcgaa ttcgtcggct
     2401 ggcggatcgt cttcaccgtg ccggtctgcc tgctgctcgt cgcggcgctg cgccagggcg
     2461 cggccctgcg cgcggcactg gattggccgg tgctgcggat gctgatgctc agcgcgttcc
     2521 tggtcggcat caactggctg gtctataccg tcgcggtcca gaccgggcac gtgatcgcca
     2581 cctcgatcgg ctattacatg aacccgctcg ccaatgtcct gttcggcacg ctgttcctga
     2641 aggagcggct cagcacccgg cagtggctgg cggtgggcct ggccgcggtg ggcgtctcgc
     2701 tgctggcctg ggacgcactg gatacgatgg ggatcagcct ggccctggcc atttcgttca
     2761 gcgcctatgg cgtggtgcgc aagctggccc cggtcgattc cctgcccggg ctgacggtgg
     2821 aaaccggggt cctgctgatc ccggccgcgc tgctggtcgg ctggttcgcg gcaagccccg
     2881 ccggcagcgc ctttggcgcg gatccggcgc tcagcgggct gatcgccata tcgggcgtag
     2941 tcaccgcggt gccgctgctg atgttcgcct atgccgccaa gcggatggac tattccgcgc
     3001 tcggcatggt ccagttcttc tcgccgacga tcgtcttctt cctggggctg ttcgtgttcg
     3061 gcgagccgct gaagcccagc cagctggcct gcttcctggt gatctggacg gccatcgcgg
     3121 tcttcatgtg ggacctctat gaccagcgcc gcaagcgcct ggcccagcta gcccaggaag
     3181 cgccagccta gcgtttcgcc ggcgtggaac ggcacgatcg gcgtgccgtt ggcgtcgatc
     3241 aggtccggca cggtgaccgg cgccttttcc agcgtcaccg tctctgcgtt gaccggcagg
     3301 ccatagaagg ccggaccgtg gagcgaggca aagccctcga accggtccag cgcgccctct
     3361 tcctcgaaga cggcgatgta gctttccagc gcaaagggcg cgttgaagat gcccgcgcag
     3421 ccgcaggacg attccttcgc gcccacggca tggggc
//
LOCUS       DASUDV010000554         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_704318,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000554 DASUDV010000000
VERSION     DASUDV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704318"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFV30_12175"
     CDS             complement(<1..586)
                     /locus_tag="VFV30_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848893.1"
                     /translation="MHPIRQHARVLTASLTGTAVEFYDFYIYATAAALVFGPLFFPAE
                     SQAAQTLFAFMSFGLAFFARPIGAIVFGHYGDRIGRKSTLVASLLLMGVSTLLIAFLP
                     TYAMVGWVAPLLLCILRFGQGFGLGGEWGGASLLAVENAPKGWEARFGSAPQFGAPVG
                     FLAANGLFLILGLTLSDADFKAWGWRIPFLASAVL"
     gene            766..1638
                     /locus_tag="VFV30_12180"
     CDS             766..1638
                     /locus_tag="VFV30_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848894.1"
                     /translation="MKAILRRLADFQSFTLAILAVVAAPLAAHAQGSKLYEFEGVQFD
                     AAERERIGAADAQLQRGVSALKRSDWATARRECSAAYETFVRVRLPDRGLGAQAYKWA
                     LACIADAQSGSGDWYMACRTYSGNGYFSLRMENARQMCVHKGAAQEAAPAATPGASQA
                     GHSEYAGTFAAFAGRLTRLNSLPGGSARTALVQELNGDCARLRGFGQRIPPAPAAAGY
                     CAGIVAFEQGRSGEACRTMWNSAGSIRTALRGQMLQEQRNHGVELQGMLDTFRPICSD
                     MGYTWPGFDQAWPH"
     gene            1690..>3230
                     /locus_tag="VFV30_12185"
     CDS             1690..>3230
                     /locus_tag="VFV30_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX4848895.1"
                     /translation="MQLPNESPADPLLDGLNPPQREAVLTTEGPVLMLAGAGTGKTAA
                     LTARLAHIVRSRLAWPSEILCVTFTNKAAREMKERVGHLVGPAVEGMPWLGTFHAIAA
                     KMLRRHAELAGLQSNYTIIDTDDQLRVLKQLIQAEGLDEKRWPARQLAGCIDRWKNKG
                     LLPQDLSAAESEAYANGQGQKFYRLYQDRLKALNACDFGDLLLHMLNILRTNREILEQ
                     YQQRFKYIMVDEYQDTNQVQYLWLRLLAQARKNICVVGDDDQSIYSWRGAEVANILKF
                     EQDFPGAKVIKLEQNYRSTPDILAAASGLIDANSQRLGKTLWTERNGGGKVRVIGIWD
                     GPEEARRIGEEAERLEREGASLATVAILVRAQFQTREFEDRFIAIGLPYKIVGGFRFY
                     ERAEVRDALAYLRLISQPSDDLAFERIYNAPKRGLGDKTLEKMHRHARARAIPLSLAA
                     LEICDTDELPARARNTLLAFIRDVARWRDLAKQTTPAELARTVLDESGYTAALQAEKS
                     AESAGR"
BASE COUNT          592 a         1098 c         1078 g          462 t
ORIGIN      
        1 ccagcaccgc gctggccagg aacggaatgc gccagcccca ggccttgaaa tcggcgtccg
       61 aaagggtgag gccgaggatc aggaacagcc cgttggcggc cagaaagccg accggcgcac
      121 cgaactgcgg cgcggacccg aaccgcgcct cccacccctt cggcgcgttc tccaccgcca
      181 gcagcgatgc cccgccccat tccccgccga ggccaaagcc ctggccaaag cgcaggatgc
      241 acagcagcag cggcgcgacc cagcccacca tggcataggt cggcaggaag gcgatcagca
      301 gggtggatac gcccatcagc agcagcgaag caaccagcgt ggacttgcgc ccgatccggt
      361 cgccgtaatg gccgaagacg atcgcgccga taggccgggc gaagaaggcc aggccaaagc
      421 tcatgaaagc gaacagggtc tgcgcggcct ggctttccgc cgggaagaac agcgggccaa
      481 agaccagcgc cgccgccgtg gcgtagatgt agaaatcgta gaattccacc gccgtgccgg
      541 taagcgaagc ggtgaggacg cgggcgtgct ggcggatcgg gtgcatggac agtgtgcttg
      601 cggatgccgg caggcgcgtc aagccgcctt gccgcctttg cggccggtga tcggggatca
      661 cacgcaggaa tggtttgacc ggaaacggct tttcgggcca aggcggcggc acggcgcgcg
      721 cgtgcaggat cgtttgttcg gtcccgcacc ggttggtgat gttcgatgaa agcgatcctg
      781 cgccgtttgg cagatttcca gtccttcact ctcgcaatcc tggccgtggt ggccgcgcct
      841 ctggccgccc atgcccaagg ctccaagctg tacgagttcg aaggcgtcca gttcgacgct
      901 gcggaaaggg agcggatcgg ggccgccgat gcccagttgc aacgcggtgt cagcgctctg
      961 aagcggagcg actgggccac ggccagacgg gagtgctccg ccgcctatga aaccttcgtc
     1021 cgggtccgct tgccggaccg cggcctgggc gcccaggcct acaaatgggc gctcgcctgc
     1081 atcgccgatg cccagtccgg cagcggcgac tggtacatgg cctgccgcac ctacagcggc
     1141 aacggctact tctcgctgcg gatggagaat gcccggcaga tgtgcgtcca caagggtgcg
     1201 gcacaggagg cagccccggc ggcaaccccg ggggcaagcc aggccggtca ttcggagtac
     1261 gcgggcactt ttgccgcctt cgccggacgc ctcacccggc tcaacagcct gcccggcggc
     1321 agcgcacgta ccgcgctggt gcaggaactg aacggcgatt gcgcccggct gcgcggcttt
     1381 ggccagcgga tccccccggc ccctgccgcg gcgggatact gcgccgggat tgtcgctttc
     1441 gaacagggcc gcagcggcga ggcttgccgc acgatgtgga acagcgccgg ctcgatccgc
     1501 accgcgctgc ggggccagat gctgcaggaa cagcgcaacc acggagtgga gctgcagggg
     1561 atgctggaca ccttccggcc aatctgcagc gatatgggct atacctggcc cggctttgat
     1621 caagcctggc cgcactgacc cccttcccca cccttgcgct gaccatcggc gttccctaga
     1681 tgttcgcagg tgcaactccc gaacgaatcc cccgctgacc cgctgcttga cgggctgaac
     1741 ccgccgcagc gcgaggccgt gctgaccacc gagggtcccg tgttgatgct ggccggggcc
     1801 ggcaccggca agaccgcggc gctgaccgcg cggctcgccc atatcgtccg cagcaggctc
     1861 gcctggccca gcgagatcct ctgcgtcacc ttcaccaaca aggccgcgcg ggaaatgaag
     1921 gagcgggtcg ggcacctggt cggcccggcg gtggaaggga tgccgtggct gggcaccttc
     1981 cacgccatcg ccgccaagat gctgcgccgc catgccgagc tcgccgggct ccagtccaac
     2041 tacacgatca tcgacaccga cgatcagctg cgcgtgctga agcagctgat ccaggccgag
     2101 gggctggacg aaaagcggtg gcccgcgcgc cagctggccg gttgcatcga tcgctggaag
     2161 aacaagggcc tcttgccgca ggacctctcg gccgccgaga gcgaagccta tgcgaacggc
     2221 caggggcaga agttctaccg gctttaccag gaccggctga aagcgctgaa cgcctgcgat
     2281 ttcggcgatc tgctgctcca catgctcaac atcctgcgca ccaaccgcga gattctggag
     2341 cagtaccagc agcgcttcaa atacatcatg gtggacgaat accaggacac caaccaggtc
     2401 cagtacctgt ggctgcgcct gctggcccag gcccgcaaga acatctgcgt ggtgggggat
     2461 gatgaccagt cgatctattc ctggcgcggc gcggaagtgg ccaacatcct caagttcgag
     2521 caggactttc ccggcgccaa ggtgatcaag ctggagcaga attaccgctc caccccggat
     2581 atcctggccg ccgcatcggg cctgatcgat gccaacagcc agcggcttgg caagacgctc
     2641 tggaccgagc ggaacggcgg cggcaaggtc cgcgtgatcg ggatctggga cgggcccgag
     2701 gaagcccggc ggatcggcga ggaggccgaa cggctcgagc gcgagggcgc cagccttgcc
     2761 actgtcgcga tcctggtccg cgcccagttc cagacccgcg agttcgaaga ccgcttcatc
     2821 gcgatcggcc tgccctacaa gatcgtcggg ggcttccggt tttacgagcg cgccgaagtg
     2881 cgcgatgccc ttgcctatct gcggctgatc agccagccca gcgacgacct ggcgttcgag
     2941 cggatctaca acgcccccaa gcgcggactg ggcgacaaga cgctggagaa gatgcaccgc
     3001 cacgcccggg cgcgggcgat cccgctgagc ctggccgcgc ttgagatctg cgataccgac
     3061 gagctgccgg cccgggcacg caacaccctc ctcgccttca tacgcgatgt ggcgcgctgg
     3121 cgcgatctgg ccaagcagac caccccagcc gaactggccc gcacggtgct ggacgaaagc
     3181 ggctacaccg ccgcgctgca ggcggaaaag agcgccgaaa gcgccgggcg
//
LOCUS       DASUDV010000555         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2096071,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000555 DASUDV010000000
VERSION     DASUDV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096071"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFV30_12190"
     CDS             <1..388
                     /locus_tag="VFV30_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4848896.1"
                     /translation="GEIELTDMAALRRRATFAYQLFPATVLVISPDYINVMTLWPQAH
                     DRTLVEDFMLIPEEPRTEKARDHWERSWTLLDGGVFGSEDFRAAALGQQGLASGAISQ
                     LTLGTLETGIHRLHEICQEQIAAAKG"
     gene            420..1169
                     /locus_tag="VFV30_12195"
                     /pseudo
     CDS             420..1169
                     /locus_tag="VFV30_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1436..1987
                     /gene="rsmD"
                     /locus_tag="VFV30_12200"
     CDS             1436..1987
                     /gene="rsmD"
                     /locus_tag="VFV30_12200"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX4848897.1"
                     /translation="MRIIAGQWRARKLQAPAGEVTRPTADRTRETLFSMLVSRLGSLE
                     GLNVADLFAGSGALGLEALSRGAASCVFVEQDPAAIRALRANIAALRAQGQCDVRAGS
                     VLALGPAKAPLDLILLDPPYNTGAGSVALDKLARLGWIGDATWISLETAADEEPRIKG
                     LEAIAERRVGKARLTLLRAPGTS"
     gene            complement(1956..>3073)
                     /locus_tag="VFV30_12205"
     CDS             complement(1956..>3073)
                     /locus_tag="VFV30_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848898.1"
                     /translation="AFMSFGLAFFARPVGAIVFGHFGDRIGRKSTLVASLMLMGVSTL
                     LIAFLPTYAMLGWVAPLLLCVLRFGQGFGLGGEWGGASLLAVEYAPRGWEARFGSAPQ
                     FGAPVGFLFANGLFLVLGLTLSDAQFTAWGWRIPFLASAFLVGLGLWVRLRIAETPAF
                     REALAREKPPAVPLAQLLGHHFGAVMAGTAGAVACFAIFYLSTAYALAQGTGSLGYPR
                     ETFLGVQLGANCFFALGILFAVIRADRVGSGTVLLQGAVATVAMGLVFGAGLQSGSLL
                     VVFATLSASLFIMGLVYGPLGSWLPSLLPVTVRYSGISLAFNLGGIIGGALAPIAAQQ
                     LAAQGHANGAGWFMSAAGAMTLAGVVWARKYQARAAG"
BASE COUNT          584 a         1088 c         1008 g          393 t
ORIGIN      
        1 gggagaaatc gagctgaccg acatggccgc gctgcgccgc cgcgcgacct ttgcctatca
       61 gctgttcccg gcaaccgtgc tggtgatcag ccccgattac atcaatgtca tgacgctctg
      121 gccgcaagcg cacgatcgca cgctggtcga agacttcatg ctgatccccg aggagccgcg
      181 gaccgaaaag gcgcgcgatc actgggaacg ctcgtggacg ctgctcgatg gcggggtgtt
      241 cggatcggag gatttccgcg ccgcggcgct gggtcagcag gggcttgcca gcggcgcgat
      301 cagccagctg acgctcggca cgcttgaaac cgggatccac cgcctgcatg aaatctgcca
      361 ggaacagatt gccgcggcca aggggtgacg cggcggcgcg gctggcgtaa ggggcggcaa
      421 tgaccacccc ttcacccgac cgcaccggcg aacggatcgc caagctcctg gcccgcgccg
      481 gcattgccag ccgccgcgag gttgaacgga tgatcgccga tggccggatc aagctgaacg
      541 gcgcggtggt ggaaacgccg gccacggtgc tgcccaacct caagggcgtc acggtcgacg
      601 accagccggt caaggcggct gaaccggccc ggctgttcgt attccacaag cccgccggtc
      661 tgatcaccgc agagcgcgat cccgccgggc ggccgacgat ctacaccgcg ctacgcaacg
      721 cgcttccgct gggcgccggg cgggtgatgc cgatcgggcg gctcgacttc aacaccgagg
      781 gactgctgct gctgaccaac gatggcgggc tcaagcgggc gatggaactg cccgccaccg
      841 gcctgccgcg cacctaccgc gcccgcacct tcggcgacat cacccaggac cggctcgagg
      901 aattgatcga ggggatcgag atcgagggcg tgcgctatgg cccgatcaac gccaacatgg
      961 aacgcagcac cggtcgcaac cagtggatcg aggtgacgat caccgagggc aagaaccgcg
     1021 aagtgcgccg cgtgctcgag catctggggc ttgaggtaag caggctgatc cgcaccaaat
     1081 acggcccctt tgaactgctc gacctgccgc gcggcgcggc ggtggaaatc cgccaggtcg
     1141 aagttgagcg gttcgtcagg aacctcaagg tgccgggcgg gacgactggc atggccgcac
     1201 cccgccatgc cgacagcatg gcccgcgaac gcgtggttcg ggcccggagc aaggccgccc
     1261 aaccggcctc ttcaaaccgc cctgctcctg ccgagcgcgc cgcgcccaag gctgccaagc
     1321 gccccttgcc gcgcaagccg cgtgacgatg gcaagctgat cgatcggccc aggcgcccct
     1381 cggccggcag gccgagcaag cgcggcgccc cgccccgcaa accgggcccg cgcaaatgag
     1441 gatcattgcc gggcaatggc gcgcccgcaa gcttcaggca ccggcaggcg aggtcacccg
     1501 ccctaccgcc gaccgcaccc gcgaaacgct gttttcgatg ctggtaagcc ggctcggcag
     1561 ccttgaaggg ctgaacgtgg ccgacctgtt cgccggctct ggcgcactgg ggctcgaggc
     1621 cctgtcgcgc ggtgcggcaa gctgcgtgtt cgtcgagcag gacccggccg cgatccgcgc
     1681 gctgcgcgcc aatatcgccg cgctgcgcgc gcagggccaa tgcgacgtgc gcgccggatc
     1741 ggtgctggcc ctgggcccgg cgaaggcgcc gctcgacctg atcctgctcg atccgcccta
     1801 caacaccggg gcgggatcgg tcgcgctcga caagctggcg cggcttggct ggattggcga
     1861 tgccacctgg atcagcctgg aaaccgccgc cgacgaggag ccgcggatca agggtcttga
     1921 agcgatagcc gaacgccggg ttggcaaggc gcggcttacc ctgctgcgcg cgcctggtac
     1981 ttcctagccc agacgactcc ggccagcgtc atcgctccgg ccgccgacat gaaccagccc
     2041 gcgccgttgg catggccttg cgcggccagt tgctgcgccg cgatcggcgc cagtgccccg
     2101 ccgatgatcc cgccaagatt gaaggccagc gaaatgccgc tgtagcgcac ggtaaccggc
     2161 agcaggctgg gaagccacga gccgagcggt ccatagacca gccccatgat gaacagcgat
     2221 gccgaaagcg tcgcgaaaac gaccagcagc gatcccgatt gcagccccgc gccgaacacc
     2281 agacccatgg ccaccgtcgc gaccgcaccc tgcaacagca ccgttcctga tcccacccgg
     2341 tcggcccgga tcactgcgaa gagaatcccc agcgcgaaga agcaattggc gcccagttgc
     2401 acacccagaa atgtctcgcg cggataaccg agcgagccgg ttccctgggc cagggcatag
     2461 gcagtcgaca gatagaagat cgcgaaacag gcaactgccc cggcggttcc ggccatgacc
     2521 gcgccgaaat ggtggcccag gagctgcgcc agcggcacgg ccggcggctt ttcgcgggcc
     2581 agggcttcgc ggaacgccgg ggtttcggcg attcgcagtc gcacccacag gccgagcccc
     2641 accaggaacg cgctggccag gaacggaatg cgccagcccc aggcggtgaa ctgcgcatcg
     2701 ctgagcgtca ggccgagcac gagaaacagc ccattggcaa acaggaaccc caccggcgcc
     2761 ccgaattgcg gggccgaacc gaaccgcgcc tcccagcccc tgggcgcata ttccaccgcc
     2821 agcagcgacg ccccgcccca ttcgccgccc aggccaaaac cctggccgaa ccgcagtaca
     2881 cacagcagca gcggggctac ccagcccagc atcgcatagg ttggcaggaa ggcgatcagc
     2941 aacgtcgaga cccccatcag catcagcgag gccacaagcg ttgacttgcg gccgatccga
     3001 tcgccgaaat ggccgaacac gatggccccc accggccgtg cgaagaatgc aagtccgaag
     3061 ctcatgaagg caa
//
LOCUS       DASUDV010000556         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2162355,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000556 DASUDV010000000
VERSION     DASUDV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162355"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..938
                     /gene="ygiD"
                     /locus_tag="VFV30_12210"
     CDS             138..938
                     /gene="ygiD"
                     /locus_tag="VFV30_12210"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX4848899.1"
                     /translation="MKHRLPALFLGHGSPMTVITDVPERRAWQALGQALPRPRAILVI
                     SAHWETNGASHVTIGSQPRTIHDFGGFPQELFDIQYPAPGADWLVERVAALLGEEQVK
                     RDASWGFDHGSWGVIRPMYPEADVPMVQLSLDRSLGAEQHLERAAALAPLRDEGVLIV
                     GSGNVVHNLRLWRQFAGTQPDWAVDFQARINRAVMAGDHAALTRFAADDQAASAAINS
                     AEHYLPLLYVVGARLPGDEAGVFSDNIDGALSMTSYLIGDTGLLGALH"
     gene            complement(935..2212)
                     /locus_tag="VFV30_12215"
     CDS             complement(935..2212)
                     /locus_tag="VFV30_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4848900.1"
                     /translation="MAAQGSSSLDRSTRVMLWTLLVVYVFNFLDRQIVNILAEPIRRD
                     LGLSDTQIGLMTGLAFALFYTVLGLPIARYADKPSTDRGRLIALALAVWSAMTALCGL
                     AQNFVQLLLARIGVGVGEAGCTPAAHSLIADRVAPEKRPGAMAFYAMGIPLGSLLGMM
                     IGGYLADTIGWRHAFMLVGTPGIILALLVTVLIKDKERVVREPAKGEVDEGLWAAVAG
                     LLRSPAYVILLGAASASSFLSYGKATWTTIFFQRSHGMSPGEVGFWFGLWGGVAGVFG
                     TWLGGWLANRFGSVDRRHIVTAPAIGMALAIPIAISAYFATDWRIALLLLMVPTVLNS
                     LYYGPVYSSAQGLVPLRHRATASALLLFGQNLIGLGLGPLFFGMLSDWLRPEFGEQSV
                     RYVLYGAACLGLIPAVLFWMLRPRLHAELDRHG"
BASE COUNT          433 a          754 c          700 g          339 t
ORIGIN      
        1 aaccggaagg cgaacacttt cgctggtggg acgaagaagc gcgcttcgtg cggctcgaag
       61 aatagcgcaa gcggcatttg cagcaggcac agctttgctt tgttggatcc ggcaagagga
      121 atcgccacct tgttcgcatg aagcaccgcc ttcccgccct gttccttggt cacggttcgc
      181 cgatgaccgt gatcaccgat gtccccgaac gccgcgcgtg gcaggcgctt ggccaggcgc
      241 tgccccggcc ccgcgcgatc ctggtgatct ctgcccactg ggaaaccaat ggggcaagcc
      301 atgtcaccat cggcagccaa ccgcgcacga tccacgattt cggcggcttc ccgcaggaac
      361 tgttcgatat ccagtatccc gctccgggtg cggactggct ggttgaacgg gttgccgcgc
      421 tgcttggcga agaacaggtg aagcgcgacg ccagctgggg gttcgatcac ggttcgtggg
      481 gcgtgatccg gccaatgtat cccgaggccg atgtgccgat ggtccagctc agcctcgatc
      541 gttcgctcgg cgccgaacag cacctcgagc gggcggcggc gctggcaccg ctacgcgatg
      601 aaggcgtgct gatcgtgggc agcggcaatg tcgtccacaa cctgcgactg tggcgccagt
      661 ttgccggaac ccagcctgac tgggcggttg atttccaggc gcggatcaat cgtgcggtga
      721 tggctggcga tcacgccgcc ctcacccgtt tcgcagctga cgatcaggcc gccagtgccg
      781 cgatcaacag cgccgaacac tacctgccgc tgctctacgt ggtcggggca aggctcccgg
      841 gcgatgaagc cggggtgttc agcgataaca tcgacggcgc cctttcgatg accagctatc
      901 tgatcggcga taccggcttg ctcggcgcgc tgcactagcc gtggcgatcc agctcggcat
      961 gcaggcgcgg gcgcagcatc cagaacagca ccgccgggat cagcccgagg caggccgcgc
     1021 cataaagcac atagcgcacc gattgctcgc cgaattcggg ccgcagccag tcggaaagca
     1081 tgccgaagaa cagcggcccc aggcccaggc cgatcaggtt ctgcccgaac agcaggagcg
     1141 cactggcggt ggcgcggtgg cgcagcggaa ccagcccttg ggccgatgaa tagaccggcc
     1201 cgtaataaag cgaattgagc accgtcggca ccatcagcag cagcagcgcg atgcgccaat
     1261 cggtggcgaa atatgccgaa atcgcgatcg ggatggccag cgccatgcca atcgccggag
     1321 cggtgacgat gtggcggcga tcaacactgc cgaaccggtt ggccagccag ccgcccagcc
     1381 aggtgccgaa caccccggcc accccgcccc acaagccgaa ccagaacccg acttcgcccg
     1441 ggctcatccc gtggctgcgc tggaagaaga tcgtggtcca ggtggccttg ccataggaca
     1501 ggaacgaact ggccgatgcc gcgccgagca ggatgacata ggccggcgaa cgcagcaggc
     1561 ctgccacggc ggcccacagc ccctcgtcga cctcgccctt ggcgggctcg cggaccacgc
     1621 gctccttgtc cttgatcagc acggtcacca gcagcgccag aatgatgccg ggtgtcccca
     1681 ccagcatgaa agcatggcgc cagccgatcg tatcggccag atagccgccg atcatcatgc
     1741 ccagcagcga ccccagcggg attcccatgg cataaaacgc catcgcccct ggacgctttt
     1801 cgggcgccac ccggtcagca atcagcgaat gggccgcggg cgtgcatccg gcttcgccca
     1861 cccccacccc gatccgcgca agcagtagct gcacgaagtt ctgagcgagg ccgcacagcg
     1921 cggtcatcgc cgaccagacc gccagcgcca gcgcaatcag tcgcccccgg tcggtgctgg
     1981 gcttgtcggc ataacgcgcg atcggcagcc ccagcacggt gtagaacagc gcgaaggcca
     2041 gcccggtcat cagcccgatc tgggtatcgc tgagccccag atcgcggcga atcggctcgg
     2101 ccaggatatt gacgatctgc cgatccagaa aattgaacac gtagaccacc agcaaggtcc
     2161 acagcatcac ccgggtcgat cgatcgagac tgctggatcc ctgcgcggcc atgttgcgat
     2221 tcctaa
//
LOCUS       DASUDV010000557         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1587905,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000557 DASUDV010000000
VERSION     DASUDV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587905"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VFV30_12220"
     CDS             <1..984
                     /locus_tag="VFV30_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848901.1"
                     /translation="NPAVNPLLALRPLQFLPPFLNIPNAVEDGKTRDSKFTWTARLAY
                     DMTDSVNLYASYATGFKASSFNLSRDSRPLARDFPALRTAGLVLPNLTTGSRFAGPEN
                     ARVYEFGIKAKWDVASANLTFFRQSITGFQSNVFTGTGFALANAGKQTSQGFEFDGMV
                     KPYADLMLTASVVYIDAKYNSFINSALGDASGKALGGVPALATTFGAQWSRELGNGDR
                     LTLRGDWHYESPVQVQEGLPGFIQRDALGQVISYDPAFAAARPFRREVSEISASATYA
                     MANGLELSVWGRNLTDNRYLVSLFDSVAQSGSISGYTNQPRTYGVTARFKW"
     gene            complement(989..>2420)
                     /locus_tag="VFV30_12225"
     CDS             complement(989..>2420)
                     /locus_tag="VFV30_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4848902.1"
                     /translation="VFAAPPLVAARHFPAMALMRARVAPLAARWRDLALPVGLGLAAI
                     VALALLTAAQPMVSLTFLGGAAVMLVLLALLGRAIRTGAARLPRPGHPILRMGLANLH
                     RPAAQTGALVTALGFGLSAFVVLAAIETSLDANIRARVPQKAPDYFVLDVPRASADAF
                     RDAVKAHAEAAQVRLVPTLRGAILAYGPRDAMVRVASLKSIPDEAWPLRGERGLTYSQ
                     AVPEGNVVTAGQWWPDNYAGEPLVSIDEQLAEALDLKLGDYLTIGVLGVERSARITSL
                     RRIDWDSLGFNYVLVFSPNALADAPHNLAATVDLPRGQDRGVLLRALVKAFPSSSVIE
                     VGGILTQARDLLRQVSLAILAAASVAVLAGIAVLLGAIAAARASRTYDNVILRVLGAS
                     GRQLLLLQLAEYALLALVLAVVALALGSGIAWLVVVQLFEFDWLPAWPQVLAVLAGGL
                     VLVLAFALAASLPLLRAKPAQALREL"
BASE COUNT          450 a          831 c          774 g          365 t
ORIGIN      
        1 aacccggcag tgaacccgtt gctggcgctg cggccgctgc aattcctgcc gcccttcctc
       61 aacatcccca atgcggtgga agacggcaag acccgcgatt ccaagttcac ctggaccgcg
      121 cgcctggcct acgacatgac cgacagcgtc aatctctatg cctcttacgc aaccgggttc
      181 aaggcgagct cgttcaacct ttcacgcgat agccgccccc tggcccgcga ctttccggcg
      241 ctgcgcacgg caggccttgt gctgcccaat ctgaccaccg gttcgcgctt tgccggcccg
      301 gaaaatgcca gggtctatga attcgggatc aaggcgaagt gggacgtggc aagcgccaac
      361 ctcaccttct tccgccagtc gatcaccggc ttccagtcga acgtgttcac cggcacgggt
      421 tttgcgctgg ccaatgccgg caagcagacc agccagggtt tcgagttcga tgggatggtc
      481 aagccctatg ccgatctgat gctgaccgcc tcggtggtct acatcgatgc caagtataac
      541 agcttcatca attcggcgct gggcgatgct tcgggcaagg cgctgggcgg ggtacccgca
      601 ctggccacca cgttcggcgc acagtggagc cgggagctgg gcaatggcga tcgccttacc
      661 ctgcgtgggg actggcacta tgaaagcccg gtccaggtcc aggaaggcct gcccggcttc
      721 atccagcgtg atgcgctggg ccaggtgatc tcctatgatc cggcctttgc cgccgcgcgc
      781 ccgttccgcc gggaagtgag cgagatctcg gcctcggcca cctatgccat ggccaatggc
      841 ctcgaactgt cggtctgggg ccgcaacctg accgacaacc gctatctcgt ctcgctgttc
      901 gattcggtag cgcagtcggg atcgatctcg ggctacacca accagccgcg cacctacggg
      961 gttaccgccc gcttcaagtg gtgagggact aaagttcgcg caatgcctgg gccggcttcg
     1021 ctctcagcaa ggggagcgaa gccgccagtg cgaaggccag gaccagcacg agcccgccgg
     1081 cgagcaccgc caatacctgg ggccaggccg gcagccagtc gaactcgaac agctggacca
     1141 ccaccagcca ggcaatcccg ctgcccagcg ccagtgccac cacggccagc accagtgcca
     1201 gcagggcata ttcggccagc tgcaacaaca gcagctgccg gccgcttgcc cccagcacgc
     1261 gcaggatcac attgtcatag gtccggctgg cccgcgccgc ggcaatcgcg cccagcagca
     1321 ccgcgatgcc agccagcacg gctaccgaag cggcggcaag gatcgcgagg ctgacctggc
     1381 gcaacagatc gcgggcttgc gtcaggatcc cgccaacctc gatcaccgag cttgacggga
     1441 aggccttcac cagcgcgcgc agcaggacgc cgcgatcctg cccgcgcggc agatcgacgg
     1501 ttgcggccag attgtgcggc gcatcggcga gggcattggg gctgaacacc agcacatagt
     1561 tgaagcccag cgaatcccaa tcgatccgtc gcagcgaggt gatcctggcg ctgcgttcca
     1621 cacccagaac gccgatcgtc aggtaatcgc ccagcttgag atcgagcgct tcggcgagct
     1681 gctcgtcgat cgaaaccagc ggttctcccg catagttgtc aggccaccac tgcccggcgg
     1741 tgacgacatt gccttcggga acggcttgcg aataggtcag cccgcgctcg ccgcgtagcg
     1801 gccaggcctc gtcggggatt gatttcaggc tggccacccg gaccatcgcg tccctcgggc
     1861 cataggccag gatcgctccg cgcagcgtcg ggacaagccg gacttgcgca gcttcggcat
     1921 gagccttcac ggcatcgcga aaggcatcgg cggacgcgcg tggtacgtcg agaacaaagt
     1981 aatccggggc cttctggggc acccgcgcgc ggatattggc atcaagactg gtttcgatcg
     2041 cggccagcac cacgaaggcg gaaagaccga agcccagcgc agtcaccagc gccccggtct
     2101 gcgcagcggg acggtgcagg ttggccagtc ccatccgcag gatcggatgg ccgggacgcg
     2161 gcaggcgcgc cgcgccggtt cggatcgccc ggcccagcag ggccagcagg accagcatta
     2221 cagcggctcc gcccaggaag gtcaggctga ccatcggctg cgcggcggtc agcagcgcca
     2281 gggcaacgat cgcggcaagc ccgagaccaa ccggcagcgc caggtctcgc cagcgagcgg
     2341 ccagcggcgc gacgcgtgcc cgcatcagtg ccatggcggg gaaatggcgc gccgcgacca
     2401 gcggcggggc ggcaaagacc
//
LOCUS       DASUDV010000558         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1853299,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000558 DASUDV010000000
VERSION     DASUDV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853299"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFV30_12230"
     CDS             <1..554
                     /locus_tag="VFV30_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848903.1"
                     /translation="GKAGVMNAGRRLAIALCALAAATGCAVSGTAAAPAAPQVLFDCR
                     DASWHVELLGTDDKRLVLVTTPLTGERRMGNAQSSWDEVRLGHVVGQGGGHQRHIRLF
                     DGVRHVILLEGVDGSLADKPGRTYAGVVTQTPANPKQDFEIACPSGRVNAKLVDNVWQ
                     WAQRVGAPLPKDEDEGGPFDGWF"
     gene            558..1028
                     /gene="ruvC"
                     /locus_tag="VFV30_12235"
     CDS             558..1028
                     /gene="ruvC"
                     /locus_tag="VFV30_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX4848904.1"
                     /translation="MLIIGLDPSLSCTGWGIVAKNGNRLSHVANGQIKTDPKLPLAAR
                     LVTLDQGLAEVITQHRPESGAVEEVFVNKNAQSTLKLGQARGVCLLALARAGLDVAEY
                     APTLVKKAVVGTGGAEKTQVQAMLGVLLPGVKLAGADAADALAVAIAHAHLLRS"
     gene            1221..1559
                     /locus_tag="VFV30_12240"
     CDS             1221..1559
                     /locus_tag="VFV30_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848905.1"
                     /translation="MHLAKTLSLAALAGLAFSGCTASRYQISDALMRYGLNENQAGCA
                     AEFLRGHLSTGQVSRLSKAARYYDKDGPLTIGDLIRVAASIDDPETTLQVGAAALACK
                     VGADIPIGRF"
     gene            complement(1567..1989)
                     /locus_tag="VFV30_12245"
     CDS             complement(1567..1989)
                     /locus_tag="VFV30_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848906.1"
                     /translation="MAVWDIDLTTRMGAREAARTASTAAFIYAGVNTLGLLILGGLVS
                     TVGNYGQTMLNLAPVALVVLAIFAGFRLRDGKGLVLGGLLAVVVALGLLIQIASLAIG
                     GGTAIGLVVLVLLVQGLRGAWALHRGTGFEDDDTAAFE"
     gene            2075..>2165
                     /locus_tag="VFV30_12250"
     CDS             2075..>2165
                     /locus_tag="VFV30_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX4848907.1"
                     /translation="MIAKLTGTLDDFGPDWAVIDVGGVGYLVQC"
BASE COUNT          359 a          735 c          741 g          330 t
ORIGIN      
        1 atggaaaagc tggggtgatg aacgcagggc gccgccttgc cattgcgctg tgcgctctgg
       61 cggcggcgac aggctgtgcg gtgagcggca cggccgccgc gccggctgcg ccgcaggtcc
      121 tgttcgactg ccgcgacgcc agctggcatg tcgaactgct tggcaccgac gacaagcgcc
      181 tggtccttgt caccaccccg ctgaccggcg aacgccgcat gggcaacgcc caatcgagct
      241 gggacgaggt ccgccttggc cacgttgtcg gccagggcgg cgggcaccag cgccacatcc
      301 ggctgttcga cggcgtgcgg cacgtgatcc tgctggaggg cgtagacggt tcgctggccg
      361 acaagccggg ccggacctat gccggagtgg tcacccagac cccggccaac cccaagcagg
      421 acttcgagat cgcctgcccc tcgggccgcg tcaacgcgaa gctggtcgac aatgtctggc
      481 aatgggcaca gcgcgtcggc gcacctcttc cgaaagacga agacgagggc gggccgtttg
      541 acggctggtt ctgacccgtg ctgatcatcg gccttgaccc ctcgctcagc tgcaccggct
      601 gggggatcgt ggcaaagaat ggcaaccggc tgagccacgt tgccaacggc cagatcaaga
      661 ccgatccgaa gctgccgctg gccgcgcggc tggtcacgct cgatcagggg ctggccgaag
      721 tgatcacgca gcaccggccc gaaagcggcg cggtggaaga ggtcttcgtc aacaagaacg
      781 cgcagtcgac gctcaagctc ggccaggccc gcggggtctg cctgctggcg ctggcccgcg
      841 ccgggctgga cgtggccgag tacgcgccga cgctggtcaa gaaggcggtg gtcggcaccg
      901 gcggggcgga gaagacccag gtccaggcga tgctgggggt gttgctgccg ggcgtgaagc
      961 tggccggggc cgatgccgcc gatgcgctgg ccgtcgcgat cgcccacgcg cacctgctgc
     1021 gcagctaggc cgcgcctcga tctccgcttg tcctgagctt gtcgaaggac tgtcctgctt
     1081 tccacgccag tttgaaaagc agtgcttcga caagctcagc acggacgggg aattgagctt
     1141 cattggccga tccggcggca agccggggtt catcaggggc gggctaagcg ggcgctttcg
     1201 ggggccaacc ggagtcgctc atgcatcttg ccaagaccct ttcgctggcc gcgctggcgg
     1261 gccttgcctt ctcgggctgc actgcctcgc gctatcagat ctccgatgcg ctgatgcgtt
     1321 atggcctcaa cgagaaccag gccggctgcg cggcggaatt cctccgcggg cacctttcca
     1381 ccgggcaggt cagccgcttg tcgaaagccg cgcgctatta cgacaaggac gggccgctga
     1441 ccattggcga cctgatccgc gtggccgcct cgatcgacga tccggaaacc acgctgcagg
     1501 tcggcgcggc ggcgctggcg tgcaaggtcg gcgcggacat tccgatcggg cggttctaga
     1561 ccggcgctat tcgaaagcgg cggtatcgtc gtcttcgaag ccggtgccgc ggtgcagggc
     1621 ccaggccccg cgcagcccct ggaccagcag gaccagcacc accagcccga tcgcggtgcc
     1681 gccgccgatc gccaggctgg cgatctggat cagcaggccc aacgcgacca cgacggccag
     1741 cagcccgccc agcaccagcc ccttgccgtc gcgcaggcga aagccggcaa agatcgccag
     1801 caccaccagc gccaccgggg ccaggttgag catggtctgg ccgtaattgc ccaccgtgct
     1861 gaccagcccg cccaggatca gcagacccag cgtgttgacc ccggcataga tgaacgccgc
     1921 ggtcgacgcg gtgcgggcgg cctcgcgcgc gcccatgcgt gtggtcagat cgatatccca
     1981 gacggccatg atgttccccc ctcaggttct tgtcccgtaa ggcgaagtgc tgccactgtt
     2041 gcaggcccgg cgcaagcctg ccatagaaca aaccatgatc gccaagctga ccggcacact
     2101 cgacgatttc ggcccggact gggcggtgat cgacgtgggc ggggtcggct acctcgtcca
     2161 gtgca
//
LOCUS       DASUDV010000559         4021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1058021,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000559 DASUDV010000000
VERSION     DASUDV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058021"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFV30_12255"
     CDS             <1..523
                     /locus_tag="VFV30_12255"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX4848908.1"
                     /translation="EAPKAAGNEGAAGKKAVAKEVKAPEPKIPIAKTPAPKASSTGSA
                     VTSGATTMGNGPGGSTKGEGVGVDGLGTGPGGGKPTKAVHTAGQINRASDFPIPPGGR
                     EARIGKSVILALTVAPSGRATGCRVYKSSGFPETDARACVLAMERLRFKPATNAAGEP
                     VTSTFYWQQKFFF"
     gene            complement(520..1065)
                     /locus_tag="VFV30_12260"
     CDS             complement(520..1065)
                     /locus_tag="VFV30_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX4848909.1"
                     /translation="MNRKILFPALALAALALSGCAAVFSKHPVGNTAVPQPAKSVELS
                     RYLGRWYELARYEQGFQKDCEGVTADYTLRDDGQIAVLNRCRKPDGRISDAKGRAKIV
                     DTATGAKLKVSFFGPFYGDYWVLDRAEDYSWAIVGEPSGRYLWILSRDPNPGKAKLAE
                     LIATAAALGYDTSMIRYTKQP"
     gene            1148..1576
                     /locus_tag="VFV30_12265"
     CDS             1148..1576
                     /locus_tag="VFV30_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX4848910.1"
                     /translation="MIAQTIQLALAPVFVLVAIGNILNLLSSRLGRVVDRSRHLQERH
                     MTTKGSDHDEVVAEIRMIDQRIRLITRAIRLMVVSGLFIGTTVAVLFLEELVALNLQR
                     VAAATFFVAVALLMWALLLFLRETQISAQQLRIPRMYLEL"
     gene            1601..2041
                     /locus_tag="VFV30_12270"
     CDS             1601..2041
                     /locus_tag="VFV30_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848911.1"
                     /translation="MTAAFSIDRETGHIRIGDTVVLCAGEHRATVEPQIIPWLEGSRD
                     HGNGFAWLDLRGLTFGSQPAHLSLCFHGGLLDQASWSVRLADAEMQSGWPTRAAIDAE
                     IAFVHRTLASEMGMAAGELAWGEVWSQFDPKGFLASNGLRYRRP"
     gene            complement(2054..2335)
                     /locus_tag="VFV30_12275"
     CDS             complement(2054..2335)
                     /locus_tag="VFV30_12275"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX4848912.1"
                     /translation="MPHTLGRDEVRRRMHARSDELAGLFPGGAQVDVSWPQEDRMALA
                     VKFMGQSVAGSIDVTETELVFDFAVPLSLAFLEPMVRGVVKDKAQKLLA"
     gene            complement(2347..3093)
                     /locus_tag="VFV30_12280"
     CDS             complement(2347..3093)
                     /locus_tag="VFV30_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848913.1"
                     /translation="MAGEAHQSDMIIGAARRSLVDQRAGGRRRSIGMGAARIRSGHYW
                     RKVRNIAVAGAVIWLGLGVIGAVIDGIGFTGLMVGVAASAAALFLFGKYPAFKMPTRE
                     DLNPADTDVRALVGRTELWLETQRRALPPPAVQLVDQIGLQLDGLAAQLAGVDQAHPK
                     AAEVRKLVGEHLPNVIDGYRKIPESLRYEDRAGSNPNQQFMGGLKTISDEIDSITRQL
                     AMGAIDNLAITTRYLDYKYGDGIDQAEAKP"
     gene            complement(3095..>4021)
                     /locus_tag="VFV30_12285"
     CDS             complement(3095..>4021)
                     /locus_tag="VFV30_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX4848914.1"
                     /translation="IRAMDKDSGVGANPAELRRTVETLDPGKQGKLSKGRKFLGIIPF
                     GNSMKRYFQSYESAQGHIQAILGHLSSGKDELLMDNAAIDVERQKLWEAMGNLEQMIH
                     ISRTLDGKLEEKAADLDATDPAKAKAIRESALFYVRQRTQDLLTQMAVTVQGYLALDL
                     VKKNNVELVKGVDRASTTTVAALRTAVTVSQAMANQRLVLEQVTALNSTTAGLIDSTG
                     QLLRDQTGKIHEMAASSTIPLETLQRAFQNIYDTMDNIDTFKVKALESMKQTVTTLTA
                     EVEKSKGYIARAEGANQAQVAGDAPTLLSLEG"
BASE COUNT          624 a         1321 c         1363 g          713 t
ORIGIN      
        1 cgaggcgccc aaggcggccg gcaacgaagg cgcggcgggc aagaaggcgg tggccaagga
       61 ggtcaaggcg cccgagccga agatcccgat cgccaagacc ccggcgccca aggccagcag
      121 caccggcagc gcggttacct cgggcgcgac aacgatgggc aacggaccgg gcggttcgac
      181 caagggcgag ggcgtcggcg tggacgggct cggcaccggg ccgggcggcg gcaagccgac
      241 caaggcggtc cacaccgccg ggcagatcaa ccgcgcgagc gactttccga tcccgcccgg
      301 cggacgcgag gcgcggatcg gcaagtcggt gatcctggcg ctgaccgtcg cccccagcgg
      361 ccgggccacc ggctgccggg tctacaagtc cagcggcttc cccgaaaccg acgcccgcgc
      421 ctgcgtgctg gcgatggagc gcctgcgctt caaacccgcc accaacgccg ccggcgaacc
      481 ggtgacctcg accttctact ggcagcagaa gttctttttc taaggctgct tggtgtaccg
      541 gatcatgctg gtgtcgtacc ccagcgccgc cgcggtggcg atcagttcgg cgagcttggc
      601 cttgccgggg ttcgggtcgc ggctgaggat ccacaggtag cggcccgaag gctcgcccac
      661 gattgcccag gagtaatcct cggcccggtc gagcacccag taatcgccat agaacgggcc
      721 gaagaagctg accttcagct tggccccggt ggcggtgtcg acgatcttgg cgcggccctt
      781 ggcgtcgctg atccgcccgt cgggcttgcg gcagcggttg aggaccgcaa tctgcccgtc
      841 atcgcgcagg gtgtagtcgg cggttacgcc ttcgcagtcc ttctggaacc cctgctcata
      901 gcgggccagt tcgtaccagc ggcccagata gcggctcagc tccacgctct tggccggctg
      961 cggcacggcg gtgttgccga ccgggtgctt ggaaaagacc gcggcgcagc ccgacagggc
     1021 aagcgcggcg agggcgaggg cgggaaacag gatcttgcgg ttcatgggac ggcctttggc
     1081 ggatgggaac tgaacatagg attgcacgcg gctttgcaca ggcgctctgc accattagac
     1141 agggggcatg atcgcccaga ccatccagct cgcgcttgct ccggttttcg tcctggtggc
     1201 gatcggcaac atcctcaatc tcctgtcgag ccggctgggc cgggtggttg accgctcgcg
     1261 gcacctgcaa gaacggcaca tgaccacaaa aggttccgac cacgacgaag tggtggccga
     1321 aatccgcatg atcgaccagc ggatccgcct gatcacccgc gcgatccggc tgatggtggt
     1381 cagcggcctg ttcatcggca cgaccgtggc ggtgctgttt ctggaagagc tggtcgcgct
     1441 caatctgcag cgcgtggcgg cggcgacgtt ctttgtggcc gtggcgctgc tgatgtgggc
     1501 gctgctgctg ttcctgcgcg aaacccagat ttccgcccag cagctgcgca tcccgcggat
     1561 gtatctggag ctttagttgc gcgagatgga gcttggccgc atgaccgccg cgttctcaat
     1621 cgaccgcgag accggccata tccggatcgg cgacacggtc gtgttgtgcg ccggagaaca
     1681 ccgggccacg gtagagccgc agatcattcc gtggctggaa ggctcccgcg atcatggcaa
     1741 cggctttgcc tggctggatc tgcgcggact gacgttcggc agccaacccg cgcacctgtc
     1801 gctgtgtttt catggcggtt tgctggatca ggcatcgtgg agcgtgcggc tggccgatgc
     1861 cgaaatgcaa tccggttggc caacgcgcgc ggccatcgac gcggaaatcg cgttcgttca
     1921 ccggacgctg gcaagcgaaa tgggaatggc ggccggtgag ctcgcttggg gcgaggtatg
     1981 gagccaattt gaccccaaag gctttctggc cagcaacggg cttcgctatc gccgcccgtg
     2041 acgttgcagg ctgtcaggcc agcagctttt gcgccttgtc cttcaccacg ccgcggacca
     2101 tcggttccag aaaagcgagc gagaggggca ccgcgaaatc gaacaccagt tcggtttcgg
     2161 tcacgtcgat cgaaccggcc accgactggc ccatgaactt caccgccagc gccatgcggt
     2221 cttcctgcgg ccagctgaca tcgacctgcg ccccgccggg gaacaggccg gccagctcgt
     2281 cgctgcgggc gtgcatccgc cggcggacct cgtcgcggcc gagggtgtgc ggcacggcca
     2341 ggcgcatcag ggcttggcct cggcctggtc gatcccgtcg ccgtacttgt aatcgagata
     2401 gcgggtggtg atcgccagat tgtcgatcgc gcccatcgcc agctggcggg tgatcgaatc
     2461 gatctcgtcg ctgatcgtct tcagcccgcc catgaactgc tggttgggat tgcttccggc
     2521 gcggtcttca tagcgcaggc tctcggggat cttgcggtac ccgtcgatca cgttgggcag
     2581 gtgctcgccc accagcttgc gcacctcggc ggccttgggg tgggcctgat cgaccccggc
     2641 cagctgcgcg gccaggccat caagctgcag gccgatctgg tcgaccagct ggaccgccgg
     2701 cgggggcagg gcgcggcgct gggtttccag ccacagctcg gtccgcccaa ccagcgcgcg
     2761 gacatcggtg tcggccgggt tgagatcctc gcgcgtcggc atcttgaagg ccgggtactt
     2821 gccgaacagg aacagcgccg cggcgctggc ggccacgccg accatcaggc cggtaaagcc
     2881 gatcccgtcg atcaccgcgc cgatcacgcc caggcccagc cagatcaccg cgccggcgac
     2941 cgcgatgttg cggaccttgc gccaatagtg gcccgagcgg atccgcgccg cgcccatccc
     3001 gatcgagcgg cggcgcccgc cggcgcgctg gtcaaccagg ctgcgccgcg cggcgccgat
     3061 gatcatgtcc gattggtgcg cttcgccggc caagtcagcc ctccaggctc agcagggtgg
     3121 gggcatcgcc ggcgacctgg gcctgattgg ccccttcggc ccgggcgata tagcccttgg
     3181 acttctccac ctcggcggtc agcgtggtca cggtctgctt catgctttcc agcgccttca
     3241 ccttgaaggt gtcgatgttg tccatcgtgt catagatgtt ctggaaggcg cgctgcagcg
     3301 tttccagcgg aatggtggag gaggccgcca tttcgtggat cttgccggtc tggtcgcgca
     3361 gcagctggcc ggtcgaatcg atcaggccgg ccgtggtgct gttgagcgcg gtgacctgtt
     3421 ccagcaccag ccgctggttg gccatcgcct ggctgaccgt caccgcggtg cgcagcgcgg
     3481 cgaccgtggt ggtgctggcg cggtccacgc ccttgaccag ttcgacgttg ttcttcttga
     3541 ccagatcgag cgcgagatag ccctgcaccg tcaccgccat ctgggtcagc aggtcctggg
     3601 tgcgctggcg gacatagaac agcgcgcttt cgcggatcgc cttggccttg gccgggtcgg
     3661 tcgcgtcgag atcggcggcc ttttcttcca gcttgccatc cagggtgcgg gaaatgtgga
     3721 tcatctgctc cagattgccc atcgcctccc acagcttctg ccgctccaca tcgatcgcgg
     3781 cgttgtccat caagagctcg tccttgccgc tggaaaggtg gccgaggatc gcctggatgt
     3841 ggccctgcgc gctttcgtag gactggaaat agcgcttcat cgaattgccg aagggaatga
     3901 tccccaggaa cttgcggccc ttggacagct tgccctgctt gcccggatcg agcgtttcga
     3961 ccgtgcggcg cagctcggcc gggttggcgc cgacgcccga atccttgtcc atcgcccgga
     4021 t
//
LOCUS       DASUDV010000560         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1499547,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000560 DASUDV010000000
VERSION     DASUDV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499547"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VFV30_12290"
     CDS             complement(<1..110)
                     /locus_tag="VFV30_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX4848915.1"
                     /translation="MKKDQVFEFFRRLAEDNPSPETELEYGNAFQLVVAVA"
     gene            complement(107..1291)
                     /locus_tag="VFV30_12295"
     CDS             complement(107..1291)
                     /locus_tag="VFV30_12295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4848916.1"
                     /translation="MAENLTPAAETARMIDSLGPEALAKAQAYTFGNHVVEVAGLGVA
                     ILVAWILIRSRVLDKVAARLSKRAWVLGTLGVSAVYLVLSDLLSLPWTVYTEWQRQHA
                     YDLSKQPLGDFLAQLLVGEAVSLVLVSLFLLGVYTLIRKTGARWWLWGGGLTAAAMLL
                     FMLAVPSVIMPLFNKYEPVPPGEVRTALEQIADDVKIPHDRIFMYDGSRQSERFTANV
                     AGIGPTARIAIADVALKEASLAEVRAVTGHEAGHYKLGHVWRSIVLMPLLAMLFFFLL
                     NRLFAPAARLLGSDATLADPRGLPVMAALAATLGLAATPVTNTLSRMGETEADIYSLQ
                     TVNEPDALASALVKTAEYRYPRPTALQEMLFYTHPSVEKRVLRAMEWKAAHPKPEAPK
                     GP"
     gene            complement(1352..2080)
                     /gene="dapB"
                     /locus_tag="VFV30_12300"
     CDS             complement(1352..2080)
                     /gene="dapB"
                     /locus_tag="VFV30_12300"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX4848917.1"
                     /translation="MARIGIIGNAGRMGQALAEAIAAAGEQHAGGIDKDGDPAALVAG
                     ADVLVDFSAPAALEANLDAAAAAGVPIVVGTTGLEERHHFLCDHAAERIAVLQTGNTS
                     LGVTLLAHLVREAASRLGEDWDIEIAETHHRMKVDAPSGTALLLGQAAAEGRGVDLAA
                     KAVRGRDGITGARAPGAIGFAALRGGSVAGDHTVHFLADDERIALTHLAENRRIFAKG
                     AVRAAQWLIGKPAGRYTMDQVLGL"
     gene            complement(2098..>2307)
                     /locus_tag="VFV30_12305"
     CDS             complement(2098..>2307)
                     /locus_tag="VFV30_12305"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEX4848918.1"
                     /translation="ASLADYRAQSAWFTAMLPAWQAHGITVLDPARVLVAGERTQIAV
                     GGVPLYFDSHHLSLAGARALIAAAP"
BASE COUNT          363 a          815 c          771 g          358 t
ORIGIN      
        1 gcgaccgcca ccaccagctg aaaggcgttg ccgtattcca gctcggtttc aggggaggga
       61 ttgtcttcgg ccaggcggcg gaagaattcg aagacctgat ccttcttcac ggacctttcg
      121 gcgcctccgg cttcgggtgc gccgccttcc attccattgc ccgtagcacc cgcttttcca
      181 ccgaggggtg ggtgtagaac agcatttcct gcagcgcggt gggccgcgga tagcggtact
      241 cggcggtctt gacgagcgcg ctggccaggg catccggctc gttcacggtc tgcagcgaat
      301 agatgtcggc ttcggtttcg cccatccggc tcagcgtgtt ggtgaccggc gttgcggcaa
      361 ggcccagcgt ggccgccagc gccgccatca ccggcaggcc gcgcggatcg gccagcgtcg
      421 catcgctgcc cagcagccgc gcggccggcg cgaacaggcg gttgagcagg aagaagaaca
      481 gcatcgccag cagcggcatg aggacgatcg agcgccagac atggccaagc ttgtaatgcc
      541 ccgcctcgtg cccggtcacc gcgcgcactt cggccagcga ggcttccttc agcgccacgt
      601 cggcaatcgc gatccgggcg gtcggcccga tcccggcgac attggcggtg aaccgttcgg
      661 actggcgtga gccatcgtac atgaagatcc ggtcgtgcgg gatcttcacg tcatcggcga
      721 tctgttccag cgcggtgcgc acttcgcccg gcgggaccgg ctcgtacttg ttgaacagcg
      781 gcatgatcac gctgggcacg gccagcatga acagcagcat cgctgcggcg gtcagccccc
      841 cgccccacag ccaccagcgc gcgccggtct tgcggatcag ggtgtagacg cccagcagga
      901 acaggctgac cagcacgagc gagaccgcct cgccgaccag cagctgcgcg aggaaatcgc
      961 ccagcggctg cttggagagg tcataggcgt gctggcgctg ccattcggtg tagaccgtcc
     1021 agggcaggct caagaggtcg ctcagcacca gatagaccgc gctaacgccc agggtgccga
     1081 gaacccaggc ccgcttcgag agccgcgccg ccaccttgtc gagcacgcgc gagcggatca
     1141 ggatccacgc gaccaggatc gcgacgccca gcccggccac ttcgaccacg tggttgccaa
     1201 aggtataggc ctgggccttg gccagggctt cgggccccag gctgtcgatc atccgcgcgg
     1261 tttcggcggc tggtgtcagg ttttcggcca tggctctccc cctcaggact gtatcgtcag
     1321 actaatacag cacgcactcc ggctggcaag gtcaaagccc cagcacctgg tccatcgtat
     1381 agcgtccggc cggcttgccg atcagccact gggccgcgcg cactgcgccc ttggcgaaga
     1441 tccggcggtt ctccgccagg tgggtcagcg cgatccgttc gtcgtcggcc aggaaatgga
     1501 ccgtgtgatc ccccgccacg ctgccgccgc gcagcgccgc aaagccgatc gcgccggggg
     1561 cccgcgcccc ggtgatcccg tcgcgcccgc gcaccgcctt cgcggcaaga tcgacccccc
     1621 gcccctcggc cgcggcctgg ccgagcagca gcgccgtgcc cgacggcgca tcgaccttca
     1681 tccggtggtg ggtttcggca atctcgatat cccagtcctc gcccagccgg ctggccgctt
     1741 cgcgcaccag gtgggcgagc agggttacac ccagcgaggt gttgccggtc tgcagcacgg
     1801 caatccgctc ggccgcgtga tcgcacagga aatggtggcg ctcttccagg cccgtggtgc
     1861 cgaccacgat cggcacgccg gccgccgcgg cggcatcgag gttggcctcc agcgcggcgg
     1921 gggcggagaa atcgaccagc acgtcggcgc ctgcgaccag ggccgccgga tcgccatcct
     1981 tgtcaatccc gccggcgtgc tgctcaccgg ccgcggcaat cgcctcggcc agggcctgcc
     2041 ccatgcgccc cgcgttgccg atgatgccaa tccgggccat gccttttccc cctgccgtca
     2101 gggcgcggcg gcgatcagcg cgcgggctcc tgcaaggctt aaatggtgcg aatcgaaata
     2161 cagcggcacg ccgccgaccg cgatctgcgt gcgttctcct gcaaccagca cgcgggccgg
     2221 gtcaagcacg gtgatgccgt gggcctgcca ggcgggcagc atggcggtga accaggccga
     2281 ctgggcgcgg taatcggcca ggctggc
//
LOCUS       DASUDV010000561         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_792957,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000561 DASUDV010000000
VERSION     DASUDV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792957"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /gene="pyrF"
                     /locus_tag="VFV30_12310"
     CDS             <1..476
                     /gene="pyrF"
                     /locus_tag="VFV30_12310"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX4848919.1"
                     /translation="TVAAAMQAIHVLEPAIVTIHASGGRAMMEDAKAAAGERTKVVAV
                     TMLTSLDESDLAATGVGGSDHDQVLRLADLAHSAGLDGIVCSGQEVGAVHRQWKDGFF
                     VVPGLRPAGGALGDQKRAVTPREARDAGASVLVIGRPISRAEDPVAAARAIEATL"
     gene            542..1291
                     /locus_tag="VFV30_12315"
     CDS             542..1291
                     /locus_tag="VFV30_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX4848920.1"
                     /translation="MNSLRFALAPIALALSLPGTIAMAQTAPVAAIAPTSTLLSVNAE
                     ARSFRKPDLAVFSAGVTSQGKTAAEALAANSADMNRVLAALKKAGIADRDIQTSNLSL
                     NPLYAPQVVGPDGRVDNPEPRIIGYQVNNQVSVKQRKLADFGKVLDTLVSVGANQVNG
                     PSFELDDSEAAMDEARTAAMAKARQRAELYAKAAGLKVKRIVSISEGGGYSPPMPVMY
                     AKAMADAAPTPIAAGEVGLTATVAVQFELAQ"
     gene            1316..1954
                     /locus_tag="VFV30_12320"
     CDS             1316..1954
                     /locus_tag="VFV30_12320"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX4848921.1"
                     /translation="MPAPRIKLCGLTDSTALDAAIAARADYAGFNFYPPSPRFLTPAK
                     AAELGARAAGRIGRVGVFVDAADAEISEAVAAGRLDAIQLHGSETPQRTAEVRSRFSL
                     PVWRVIGVAKAEDIAKATAYAGAADFILFDAKTPKGTLPGGMGLAFDWNLLAGLKIAQ
                     PWGLAGGLTAANIGDAVRITGAPLVDAASGIESAPGVKDPALIAAFCAAARA"
     gene            complement(2012..2473)
                     /locus_tag="VFV30_12325"
     CDS             complement(2012..2473)
                     /locus_tag="VFV30_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848922.1"
                     /translation="MRKILIALAATAAFASPALANEARVEARGGVIWSDGDSQDFWGM
                     AAGYDFDLGEKAFAGLEVSGDKIGTSGTKVAFGVTGRLGAKVSEKGKLFVDGGYTTEP
                     CDLCEDSTHAGVGFEQGFGNNLYGKIGYRHFFTGNGVSDSDAVTVGLGVKF"
     gene            2708..>3020
                     /locus_tag="VFV30_12330"
     CDS             2708..>3020
                     /locus_tag="VFV30_12330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978640.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX4848923.1"
                     /translation="MNTANSYRNQPDERGHFGQFGGRYVAETLMPLVLDLEREYRAAK
                     ADPAFHAEFDDLLEHYVGRPSPLYFAPRLTEELRKDAPAGKGAQIWFKRDELNHTGAH
                     KI"
BASE COUNT          526 a         1025 c          978 g          491 t
ORIGIN      
        1 acaccgtcgc agccgcaatg caggcgatcc acgtgctgga gccggcgatt gtcacgatcc
       61 acgcgtcggg cggccgcgca atgatggaag atgccaaggc ggcggcgggc gaacggacca
      121 aggtggtcgc ggtcaccatg ctcaccagcc tcgatgaaag cgatctggcc gccaccggcg
      181 tgggcggatc ggaccatgac caggtgctgc gcctggcgga tctggcgcat tcggccgggc
      241 tcgacgggat tgtctgttcc ggccaggaag ttggcgcggt tcaccgccag tggaaggacg
      301 ggttcttcgt cgtccccggg ctgcgtcccg ccggcggcgc gctaggcgat cagaagcgcg
      361 ccgttacccc gcgcgaggca cgcgatgccg gggcttcggt gctggtgatc ggccgcccga
      421 tcagccgggc tgaggatccc gtggccgccg cgcgggcgat cgaggcaacg ctttaggccc
      481 ttctccggtt cagccgggcg aaagctggcc gggcgcatgg ctgcgagcca aggagaagac
      541 catgaattcg cttcgtttcg cgcttgcccc catcgccctt gccctgtcct tgccgggaac
      601 tattgccatg gcccagactg cgccagttgc cgccattgct cccacctcca ccctgctctc
      661 ggtcaatgcc gaggcgcgtt ccttccgcaa gccggacctg gccgtgttca gcgccggggt
      721 gaccagccag ggcaagaccg ccgccgaggc gctggccgcc aattcggccg acatgaaccg
      781 cgtcctcgcc gcgctcaaga aggccgggat cgccgaccgc gatatccaga ccagcaatct
      841 cagcctcaac ccgctctatg ccccgcaggt cgtcggcccg gatggccggg tcgacaaccc
      901 cgagccgcgg atcatcggct atcaggtcaa caaccaggtc tcggtcaagc agcgcaagct
      961 ggccgatttc ggcaaggtgc tcgatacgct ggtcagcgtt ggcgccaacc aggtcaacgg
     1021 ccccagcttt gaactcgacg atagcgaggc cgcaatggac gaagcccgca ccgccgccat
     1081 ggccaaggcc cgccagcgcg ccgagctcta tgccaaggcc gctggcctca aggtcaagcg
     1141 gatcgtttcg atcagcgagg gcggcggcta cagcccgccg atgccggtga tgtacgccaa
     1201 ggccatggcc gatgccgcgc ccaccccgat cgcggcgggc gaagtcggcc tgaccgccac
     1261 agtcgcggtg cagttcgaac tggcacagta aaggtccacg cgttaggggg cggcaatgcc
     1321 cgccccccgg atcaagctct gcggattgac cgacagcacc gcgctggacg cggcgattgc
     1381 cgcgcgcgcc gactatgccg gcttcaactt ctacccgcct tccccgcgct tcctgacgcc
     1441 tgccaaggcg gcagagctgg gcgcgcgggc tgcgggcagg attggccggg tcggggtctt
     1501 cgtcgatgcc gctgacgccg aaatttccga ggcggtagcc gccggtcggc tcgatgccat
     1561 ccagctccac ggcagcgaaa cgccgcagcg cacggccgag gtccgcagcc gattcagcct
     1621 gccggtctgg cgcgtaatcg gggtggccaa ggcagaagat attgccaagg ctaccgccta
     1681 tgccggggcc gccgatttca tcctgttcga cgccaagacg cccaagggca ccctgcccgg
     1741 cgggatgggc ctggcctttg actggaacct gctggcgggg ctgaagatcg cccagccctg
     1801 gggcctcgcg ggtggcctta ccgccgccaa tattggcgat gcggtgcgga tcaccggcgc
     1861 gccgctggtc gatgcggcat cggggatcga gagcgccccc ggcgtgaaag acccggcgct
     1921 gatcgcggca ttctgcgccg ctgctcgcgc ctgacgcaaa aaggggcggc ccgtcgccgg
     1981 accgcccctc gctcggtgga ctgaaaagcc cttagaactt gacgcccagg ccaaccgtca
     2041 cggcatcgct gtcgctcacg ccgttgccgg tgaagaagtg gcgatagccg atcttgccat
     2101 agaggttgtt gccgaagccc tgctcgaagc cgacgccagc gtgcgtcgaa tcttcgcaca
     2161 ggtcgcacgg ctcggtggtg tagccgccgt caacgaacag cttgcccttt tccgaaacct
     2221 tggcgccgag gcggccggtc acgccgaagg caaccttggt gcccgaggta ccgatcttgt
     2281 cacccgagac ttcgaggccg gcaaaggcct tctcgcccag gtcgaaatcg tagccggcgg
     2341 ccatgcccca gaagtcctgg ctgtcaccgt cgctccagat tacgccgccg cgggcttcga
     2401 cgcgggcttc gttggcgagc gcaggcgaag caaatgccgc agtagcggcc agggcgatga
     2461 gaatcttgcg cattgaatgt actccttgat tttggtcccc gggttgcagg gaacgggccg
     2521 gagctaggca tcgccacctt gcattcaagt gaacaggccg ttcagaaaat acaacaactt
     2581 gttcacctgt ggcacttgtg caacattttg tttcgttcat ctggcagaaa gtttcggttg
     2641 gcgcaggcgg aaatccgggc cttgcggacc gctggacagc gcctgcaatc cctgccaagc
     2701 gcccggcatg aacacggcca attcctatcg caaccagcct gacgagcgcg gccatttcgg
     2761 gcagttcggc ggccgctatg tcgccgaaac gctgatgccg ctggtcctgg accttgagcg
     2821 ggaatatcgc gccgccaagg ccgatccagc cttccatgcc gagttcgacg atctgctgga
     2881 gcattacgtc ggccgcccca gcccgctcta tttcgctccg cgcctgaccg aagagctgcg
     2941 caaagacgcg cccgccggaa aaggcgcaca aatctggttc aagcgcgatg aactgaacca
     3001 taccggcgcg cacaagatca
//
LOCUS       DASUDV010000562         6000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1234884,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000562 DASUDV010000000
VERSION     DASUDV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234884"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFV30_12335"
     CDS             complement(<1..546)
                     /locus_tag="VFV30_12335"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX4848924.1"
                     /translation="MWGARGLVLPGIQERDWRRARLFGPIVALTLGVLGLLVLLSFFL
                     VERFDKVASDREVTMVRYGFSREVGELNQVVATQVEWDDAIASLDRKRDFEWADFNVG
                     SYLYTFHGISHAFVIDPEGQAFYAALNGERAAPESYAPFAAPASRLIPAIRSAEARRP
                     PLRERAGKNNIDIPSIQANTVA"
     gene            911..1711
                     /locus_tag="VFV30_12340"
     CDS             911..1711
                     /locus_tag="VFV30_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX4848925.1"
                     /translation="MDLKDMFDPDGEIPPATPASTLVIFRNCPQGGAPQLLMVQRSKE
                     MRFAGGAAVFPGGRVDDADRDLAAALGAGDEETRDELAARIASVRETLEETGLVVGIA
                     QRPSLDEARAARAHLLEIGTLAPVLDAMGWSLDPDALVPFSRWRPKHREIRVFDTRFY
                     LTNLGTGAVDLLVDATENTRLFWASASEALRLADEGEIKVIFPTRRNLERLAQFDHYD
                     AALAHVAEYPSKMITPFMEVRDGVPWLLIPDDAGYPVAGEVLDTAARG"
     gene            complement(1733..1809)
                     /locus_tag="VFV30_12345"
     tRNA            complement(1733..1809)
                     /locus_tag="VFV30_12345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1773..1775),aa:Arg,seq:tct)
     gene            1872..2660
                     /locus_tag="VFV30_12350"
     CDS             1872..2660
                     /locus_tag="VFV30_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX4848926.1"
                     /translation="MTGTIQQIDGVSGARRHFRYFDYVMAAFVAILLLSNLIGAAKLA
                     VADLSWLTGWTGWLPWHWLGIGPDGLMIFGAGILFFPLSYVIGDVLTEVYGYANARRC
                     VWMGFGALIFMAFMSYVVVAMPPAAGWDGQAAYQSVFGSTWRIVLASILAFWAGEFVN
                     SFVLAKMKIWTKGKHLWSRTIGSTVFGQGVDSLIFYPVAFLGIWTTDQVLTVMVTNWF
                     LKVLWEALLTPVTYAVVGWFKAREGVDVFDSGTDFSPFAKAEAV"
     gene            complement(2712..2927)
                     /locus_tag="VFV30_12355"
     CDS             complement(2712..2927)
                     /locus_tag="VFV30_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX4848927.1"
                     /translation="MTPINTVENDMTTNRSFRLDKTNGKFMGVCSGIANYFGWDANLV
                     RIGFALGTIFGFGSLILVYLAIGLIAD"
     gene            complement(2924..3064)
                     /locus_tag="VFV30_12360"
     CDS             complement(2924..3064)
                     /locus_tag="VFV30_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848928.1"
                     /translation="MTQQSTFTSKLAAAASAFVLSLVLITGTVSMPQTAQAQTVYVGD
                     LA"
     gene            3284..4357
                     /gene="recF"
                     /locus_tag="VFV30_12365"
     CDS             3284..4357
                     /gene="recF"
                     /locus_tag="VFV30_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX4848929.1"
                     /translation="MGLDRITLSQFRNHRATRLEGTARFNLLVGDNGAGKTNVLEAIS
                     LLAPGRGLRRAALPDIPAKDGDGSFAVSADLAGAVTLGTGVRADRPSRRVVQVNGAEV
                     PAVRLGEWLAIGWLTPAMDRLFAEGAGARRRFLDRMVLALEPGHARTAAKLEGALRER
                     NRLLAEEREPDPQWLDAIETQLAEAGAAVAQARARLVARLGEALDGLPEAPFARPALA
                     YHPGGPTEAAALAAALAENRRRDRAAQRTLTGPHRDELAVTMAGKDQPAADCSTGEQK
                     AMLIAITLAHAGLLDPDRPGVLLLDEVAAHLDPVRREALFDRLRAGAAQVWLTGTELA
                     PFDAIAAEAATWRVSEGLAERLA"
     gene            complement(4314..4937)
                     /locus_tag="VFV30_12370"
     CDS             complement(4314..4937)
                     /locus_tag="VFV30_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX4848930.1"
                     /translation="MPDLPVVGEEVTIVALGDSLFAGYGVEPGQSYPARLEAALRARG
                     INARIVNAGVSGDTTSGGLQRVDFVLSGLKAPPALVIISLGGNDMLRGLPPAETRANL
                     DAILGKLAARKIPVVVMGMLSAPNWGADHAKAFNAIYPELAKKHGAALVPFFLQAVID
                     KPELIQADRIHPTAQGLEEMVVATIDPVAKALPVRPAAPPVPRSPAK"
     gene            5099..5791
                     /locus_tag="VFV30_12375"
     CDS             5099..5791
                     /locus_tag="VFV30_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4848931.1"
                     /translation="MTTPELAIDARNLTLTLGQGDSAVDVLRGIDLAVPLGQTLALLG
                     PSGSGKSSLMAVLSGLERASGGALSVAGENFAALDEDALATARRGRIGVVLQAFHLLP
                     TMTAIENVMTPLELAGLPDARARAEAELTAVGLGHRLNHYPAQLSGGEQQRVAIARAL
                     AARPALVFADEPTGNLDAATGATIMDLLFARRAEAGATLVIITHDASLAERCERVVTL
                     ADGRIASDSAAA"
     gene            5788..>6000
                     /locus_tag="VFV30_12380"
     CDS             5788..>6000
                     /locus_tag="VFV30_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848932.1"
                     /translation="MIGWRAAWTIARRDLSARFRGLRLLLVCLFLGVGALAAIGTLTG
                     AIGNELSSRGRTILGGDVEIAVWQRSA"
BASE COUNT          917 a         2000 c         2082 g         1001 t
ORIGIN      
        1 ggcgaccgtg ttggcctgga tcgaggggat gtcgatgttg ttcttgccgg cccgttcgcg
       61 cagcggcggg cggcgggcct cggcgctgcg gatcgccggg atcagccgcg aggccggggc
      121 cgcgaagggg gcatagctct caggcgcggc gcgctcgccg ttcagcgcgg catagaacgc
      181 ctgcccctcg ggatcgatca cgaaggcgtg gctgatgccg tggaaggtgt agagatagct
      241 gccgacgttg aaatcggccc attcgaagtc gcgcttgcga tccaggctag caatcgcgtc
      301 gtcccattcg acctgggttg cgaccacctg gttcaactcg ccgacctcgc gcgagaagcc
      361 atagcggacc atggtcactt cccggtcgga cgcgaccttg tcgaaccgtt ccacgaggaa
      421 gaacgagagc aggaccagca ggccgagcac gccaagggtc agggcaacga tcggcccgaa
      481 cagccgtgcc ctgcgccaat cccgttcctg aatgcccggc agaaccaatc cgcgagcccc
      541 ccacacgttt ggcggccgcg gaaccgcatt tccgtggcca atccgcgagc catatgcggc
      601 ggacgtgaag aaagcgttgc cacccgatcc gggccgcggc gcggttttgc agcgattttc
      661 gggggagttc tcggggaggc ggggccgcgg agaccagggt gcggccccgc ctcattcgag
      721 actgcttggg ggtatcccag gtaatgcaca ggccgtgcca aaatcgcctt ttcgtgtttt
      781 ttccgtaatt tacggattcg caaggattcg cgccacagcc cgggtgtaag aatatccgac
      841 aatttcggcc cgctccggcc ccgcgctttt gcgcttggca aagccgcaac aaggtttcac
      901 cttgccaccc atggacctca aagacatgtt cgatccggac ggcgaaattc cgccggccac
      961 tccggcttcc accctggtga tctttcgcaa ctgcccgcag ggcggcgcgc cgcagctgct
     1021 gatggtgcag cgatccaagg aaatgcgctt tgccggcggc gcggcggtgt tccctggcgg
     1081 gcgggtcgac gatgccgacc gcgacctggc cgcggcgctg ggcgcaggcg acgaggagac
     1141 gcgtgacgaa ctggcggcgc ggatcgcctc ggtccgcgaa acgctggagg aaaccgggct
     1201 ggtggtgggc attgcccagc gccccagcct ggacgaagcc cgcgccgccc gcgcgcacct
     1261 gcttgagatt ggcaccctgg ccccggtgct ggatgcgatg ggctggtcgc tcgatcccga
     1321 tgcgctggtc ccgttctcgc gctggcggcc aaagcaccgc gaaatccggg tgttcgacac
     1381 ccgtttctac ctgaccaacc tgggcaccgg cgcggtcgac ctgctggtgg acgcgaccga
     1441 gaacacccgc ctgttctggg ccagcgccag cgaagcgctg cggctggccg acgagggcga
     1501 gatcaaggtg atcttcccga cccgccgcaa cctggaacgg ctggcccagt tcgatcacta
     1561 cgacgccgcg ctggcccatg tggcggaata cccgtccaag atgatcaccc ccttcatgga
     1621 agtgcgcgac ggggtgccgt ggctgctgat cccggacgac gccggctatc cggtggcggg
     1681 cgaagtgctc gacaccgcgg cgcggggata acagacacca agtctgggga tttggcgcgc
     1741 ccgggtggag tcgaaccacc ggcctcaaga ttagaagtct cgtgctctat ccagctgagc
     1801 tacgggcgcg cggcgggtgt cctcataggc aggctttccg gccagcgcaa atggcgctag
     1861 cctgctttcc catgacgggg accatccagc agatcgacgg cgtttcgggc gcccggcgcc
     1921 atttccgcta tttcgactat gtgatggcgg ccttcgtcgc gatcctgctg ctgtcgaacc
     1981 tgatcggggc ggccaagctg gccgtggccg atctgtcgtg gctgaccggg tggaccggat
     2041 ggctgccgtg gcactggctg gggatcggcc cggacggcct gatgatcttt ggcgcgggga
     2101 tcctgttctt cccgctgtcc tatgtgatcg gcgacgtgct gaccgaggtt tacggctatg
     2161 ccaacgcccg gcgctgcgtg tggatgggct ttggcgcgct gatcttcatg gccttcatga
     2221 gctatgtcgt ggtggcgatg ccgccggccg ccgggtggga cgggcaggcc gcctatcaaa
     2281 gcgtgttcgg cagcacctgg cggatcgtgc tggcctcgat cctggccttt tgggcggggg
     2341 aattcgtcaa cagcttcgtg ctggccaaga tgaagatctg gaccaagggc aagcacctgt
     2401 ggagccggac gatcggctcg actgtcttcg gccagggggt ggacagcctg atcttctatc
     2461 cggtcgcctt cctcgggatc tggacgaccg accaggtgct gaccgtgatg gtcaccaact
     2521 ggttcctgaa agtgctgtgg gaagcgctgc tgacgccggt gacctatgcc gtggtcggct
     2581 ggttcaaggc ccgcgagggc gtggacgtgt ttgacagcgg gaccgacttt tcgccctttg
     2641 ccaaggccga agcggtctga agggtccggt gtcaggcccc gcccgcaggc ggggcctcac
     2701 cggcgatccc gtcaatcggc gatgaggccg atcgcgaggt agaccaggat cagcgatccg
     2761 aagccgaaga tcgtgccgag ggcgaagccg atccggacca ggttggcatc ccagccgaag
     2821 tagttggcga tgccgctgca cacgcccatg aacttgccgt tggtcttgtc gaggcggaaa
     2881 gagcggttgg tggtcatgtc gttctccacg gtgttgatcg gggtcatgcg aggtctccca
     2941 cgtaaacggt ctgggcctgg gcggtctgcg gcatcgagac ggtgccggtg atcaggacca
     3001 gcgaaaggac aaaagccgaa gccgccgcgg caagcttgct ggtgaaagtg ctctgttggg
     3061 tcatttccat ttccttcttc aatctgttca gcggagcgat ccgccgatgc cagactgatt
     3121 gcaggggccg tgccaatttc ggaaaagcgc ggaaaatcaa gctttccaaa tcctgaacgg
     3181 ccagttggct ttcatgcagg aaacaacagt tggtgaaatt tcccaacttt caggatcacg
     3241 ctttggggtg acagcgcccc ctcgctccct ctatcgccgc accatggggc tcgaccgcat
     3301 caccctcagc cagtttcgca accatcgcgc cacccggctc gagggcacgg cgcggttcaa
     3361 cctgctggtg ggcgacaacg gcgcgggcaa gaccaacgtg ctggaggcga tctcgctgct
     3421 ggccccgggc cgcggcctgc gccgggccgc cctgcccgac attccggcca aggacggcga
     3481 cggcagcttt gccgtcagcg ccgatctggc cggggcggtg accctgggca ccggggtccg
     3541 cgctgatcgg cccagccgcc gggtggtgca ggtgaacggg gccgaggttc cggccgtgcg
     3601 gctgggcgaa tggctggcga tcggctggct gaccccggcg atggaccggc tcttcgccga
     3661 aggggccggc gcgcggcggc ggttcctgga ccggatggtc ctggcgctgg agccggggca
     3721 cgcccggacg gcggccaagc tggaaggggc gctgcgcgaa cgcaaccggc tgctggccga
     3781 agagcgcgag cccgatccgc aatggctgga cgcgatcgag acgcagctgg ccgaggccgg
     3841 cgccgcggtg gcccaggccc gtgcccggct ggtcgcccgg ctgggggagg cgctggacgg
     3901 cttgcccgaa gcgccctttg cccgcccggc cctggcctat caccccggcg gaccgaccga
     3961 agcggcagcg ctggccgccg cactggccga aaaccgccgc cgcgaccgcg ccgcccagcg
     4021 caccctgacc gggccgcacc gcgacgagct ggccgtgacc atggccggca aggatcagcc
     4081 cgcggccgat tgctccaccg gcgagcagaa ggcgatgctg atcgcgatca cgctggccca
     4141 cgccgggctg ctcgatccgg accggccggg tgtgctgctc ctcgacgaag tggcggccca
     4201 cctcgatccg gtccggcgcg aagccctgtt cgaccggctc cgcgccgggg cagcgcaggt
     4261 ctggctgacc ggaaccgagc ttgcgccgtt tgacgcgatc gccgccgaag cggctacttg
     4321 gcgggtgagc gagggactgg cggagcggct ggcctgaccg gcagggcttt cgccaccggg
     4381 tcgatcgtcg ccacgaccat ttcctccagc ccctgcgcgg tcggatggat ccggtcggcc
     4441 tggatcagct cgggcttgtc gatcaccgcc tggaggaaga acggcaccag cgcggcgccg
     4501 tgcttcttcg ccagttcggg atagattgcg ttgaaggcct tggcatggtc cgcgccccag
     4561 ttcggcgcgg agagcatacc catcaccacc accgggatct tgcgcgccgc cagcttgccc
     4621 aggatcgcat ccaggttggc gcgggtttcc gccggcggca ggccgcgcag catgtcattc
     4681 ccgcccaggc tgatgatcac cagcgccggc ggggccttca gcccgctcag cacgaaatcg
     4741 acccgctgca gcccgcccga ggtcgtatcc cccgaaaccc cggcgttgac gatccgggcg
     4801 ttgatgccgc gcgcgcgcag cgccgcttcc agccgcgcgg gatagctttg ccccggttcc
     4861 accccgtatc cggcgaacag gctgtccccc agcgccacga tcgtcacctc ttcgcccacc
     4921 accggcaggt cgggcattgc tgccgcgctt tcggccgcga cgggcgtcgc ggccttctcc
     4981 gagccgcatg ccccgagaga cagggttgca actgcgacca tcaatttcca tttcatagag
     5041 gaactctcgc tctgttcttc actgggcatt ccccctctag ctatggcatc ccccgcgcgt
     5101 gacaacccct gaactggcga tcgacgcccg aaaccttacc cttacccttg gccagggcga
     5161 cagcgcggtg gacgtgctgc gcgggatcga tctggccgtg ccgctgggcc agacgctggc
     5221 gctgctgggc ccgtcgggtt cgggcaagag cagcctgatg gcggttttgt ccggtctgga
     5281 acgggccagc ggcggcgcgc tgagcgtggc gggcgagaat ttcgccgcgc tggacgagga
     5341 cgccctggcc acggcccggc gcgggcggat cggcgtggtg ctgcaggcct ttcacctgct
     5401 gccgaccatg accgcgatcg aaaatgtgat gaccccgctg gaactggccg gcctgcccga
     5461 tgcccgcgcc cgggccgagg cggagctgac cgcggttggc ctcgggcacc ggttgaacca
     5521 ctatccggcc cagctttcgg gcggcgagca gcagcgcgtg gcgattgccc gcgcgctggc
     5581 cgcccggccc gcgctggtct ttgcggacga gccgaccggc aacctggatg ccgccaccgg
     5641 ggccacgatc atggacctgc tgttcgcccg ccgggccgaa gccggggcga cgctggtgat
     5701 catcacccac gatgccagcc tggccgagcg ctgcgagcgc gtggtcaccc tggctgacgg
     5761 ccgcatcgcc agcgacagcg cggcggcatg atcggctggc gcgccgcctg gacgatcgcc
     5821 cggcgcgatc tgtcagcccg gttccgcggg ctgcggctgc tgctggtctg cctgttcctg
     5881 ggcgtcggcg cgctggccgc gatcggcacc ctgacgggcg cgatcggtaa cgaattgtcg
     5941 agccggggca ggaccatcct gggcggcgac gtcgagattg cggtctggca gcgttcggcc
//
LOCUS       DASUDV010000563         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1013759,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000563 DASUDV010000000
VERSION     DASUDV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013759"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1425
                     /locus_tag="VFV30_12385"
     CDS             277..1425
                     /locus_tag="VFV30_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4848933.1"
                     /translation="MGTRLSLDQFDRTVADIYEAALDPAHWDVAMGGIVNRNAPTGWE
                     VAFLIWETIAPPSGRFVGAFGVNEFARAGYLTAFAGRQPWSIHAHGLPVGTLVHSDEL
                     LPREQFKACDLYQRFLSSWGLEAALIGIVDRAGPQRLGLVLPGGADDPGPLLYEAVRR
                     YLPHIQRATRISRKLGEANLRAENAEAVLQRTPNVTLVLGADLGVQFANHRAESFLVQ
                     GHADAPGGRLRLRNPRAQAALQALADGSDPAPSAAFVLEADGLPPYRMLAMRIAAPIA
                     ETLCGPVEGGAVLLVGHAHAGGVPIETLDNYASWFNLTPTEARLAAMLAQGHSLEDYA
                     RNRAVTVNAGRFLLKSIFAKTGATRQAELVALLRDAPDGWIEQREAAD"
     gene            1529..>2261
                     /locus_tag="VFV30_12390"
     CDS             1529..>2261
                     /locus_tag="VFV30_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848934.1"
                     /translation="MRFQTALMTLSALLAASAAAVSAQDVDQVVHMDGPGVTAVPAQR
                     VAQPVSNPLTAAQQKAISQGKALRPGVYRLRSVHSGGCLATNFAVPRFLFQLSSCGTE
                     LIGNMYYGDDGLNLAILPHPAGGYTVRSAHPAYLGWVQNGLRAPVNGEYGNCLTVARG
                     VILGAPRIELLPCDSAGDWTRTGADDQRFFFLPSGPGTYQIALALTGDGTDCWALRNG
                     SRDSQTDVIRWQCNRTADQMWEPRWI"
BASE COUNT          340 a          796 c          791 g          334 t
ORIGIN      
        1 gatcaccggc ccggtatagg ccgcgggtgc cgcaggcggc ggcacggcgg gcgcgggggc
       61 ggcctgatcc ggctgctccg cggcgggagg cgggctctcc gatccgcaag ccgcgagcgc
      121 ggccgatgca gccagaacaa ggacgaagcg ggacatggac acctccgggc aggactggaa
      181 cgatctgcag cctgcccgag ggcgcgcggc gccgcgctat tcatttgggt agtccggctt
      241 gcccgccccg attcggcagg atattcagtg tcagcaatgg gtacgcgcct ctctctcgac
      301 cagttcgacc ggacggtggc cgacatttac gaggccgcgc tcgatccggc gcactgggac
      361 gtggcgatgg gcgggatcgt caaccgcaac gcgccgaccg gctgggaagt ggccttcctg
      421 atctgggaaa cgatcgcccc gcccagcggc cggttcgtcg gcgccttcgg agtcaacgaa
      481 ttcgcccggg ccggatacct gaccgccttt gccggacgcc agccgtggag catccatgcc
      541 cacggcctgc cggtcggcac gctggtccac agcgacgagc tgctcccgcg cgaacagttc
      601 aaggcctgcg acctctacca gcggttcctg tcgagctggg ggctggaagc cgccctgatc
      661 gggatcgtcg accgcgccgg gccgcagcgg ctggggctgg tcctgcccgg cggggcggac
      721 gatcccgggc cgctgctcta tgaagcggtg cgccgctatc tgccgcacat ccagcgcgcc
      781 acccggatca gccgcaagct gggcgaggcg aacctgcggg ccgaaaacgc cgaagccgtg
      841 ctgcagcgca cgcccaacgt caccctggtg ctgggggccg atctgggcgt ccagttcgcc
      901 aaccaccggg ccgaatcctt tctggtccag ggccatgccg acgcgccggg cgggcggctg
      961 cggctgcgca acccgcgggc ccaggccgcg ctgcaggcgc tggccgacgg cagcgatccc
     1021 gcgccgagcg ccgccttcgt gctggaggcc gatggcctgc cgccctaccg gatgctggcg
     1081 atgcggatcg ccgcgccgat tgccgaaacg ctgtgcgggc cggtggaagg cggcgcggtg
     1141 ctgctggtcg gccacgccca tgccggcggg gtgccgatcg aaacgctgga caactatgcc
     1201 agctggttca acctgacccc caccgaagcc cggctggcgg cgatgctggc ccagggccat
     1261 tcgctggaag actatgcccg caaccgcgcg gtcacggtca acgccgggcg gttcctgctg
     1321 aaaagcatct ttgccaagac cggggcgacc cgccaggccg aactggttgc cctgctgcgc
     1381 gacgcgcccg atggctggat cgaacagcgc gaagccgctg actgaccgtg aatttccgct
     1441 tctgctgatt tcgccctact gatttgggta gcgccagacg gcgcggcccg gcgcaggctg
     1501 cctcccgaac caatccggga gaaccgccgt gcgtttccag actgccctga tgaccttgtc
     1561 cgcgctgctg gccgccagcg ccgctgccgt atcggcgcag gatgtcgacc aggtggtcca
     1621 catggacggg ccgggcgtga ccgccgttcc ggcgcagcgg gtggcccagc cggtcagcaa
     1681 cccgctcacc gcggcgcagc agaaggcgat ctcccagggc aaggcgctgc gccccggtgt
     1741 ctaccggctg cgttcggtcc attcgggggg ctgcctggcg acgaactttg ccgttccccg
     1801 gttcctgttc cagctgtcat cctgcggaac cgaactgatc ggcaacatgt actacggcga
     1861 cgacggcctc aatcttgcaa tcctgccgca cccggcgggt ggctacacgg tgcgcagcgc
     1921 ccatccggca tacctcggct gggtccagaa cggcctgcgc gcgccggtga acggggaata
     1981 cggcaactgc ctgaccgtgg cgcgcggcgt gatcctgggg gcaccgcgga tcgaactgct
     2041 gccgtgcgac agcgccggcg actggacgcg gaccggggcg gacgaccagc gtttcttctt
     2101 cctgccctcc ggacccggca cctatcagat cgcgctcgcg ctgacgggcg atggcaccga
     2161 ttgctgggcg ctgcggaacg gatcgcgcga ttcgcagacc gacgtgatcc gctggcaatg
     2221 caaccgcacc gccgaccaga tgtgggaacc gcgctggatc g
//
LOCUS       DASUDV010000564         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_262495,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000564 DASUDV010000000
VERSION     DASUDV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262495"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VFV30_12395"
     CDS             complement(<1..96)
                     /locus_tag="VFV30_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEX4848935.1"
                     /translation="MSAPAVLFVCLGNICRSPLAEAAFRAEAAAAG"
     gene            complement(93..368)
                     /locus_tag="VFV30_12400"
     CDS             complement(93..368)
                     /locus_tag="VFV30_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848936.1"
                     /translation="MDLDALILHFFEVEDPASLSEAEFERGLERLRIGFGVEREPGRR
                     FALWTLMEALGVAPLPAEAFAKEPALKAAAEDYLKAAFRLERYGEGK"
     gene            complement(373..1923)
                     /gene="ubiB"
                     /locus_tag="VFV30_12405"
     CDS             complement(373..1923)
                     /gene="ubiB"
                     /locus_tag="VFV30_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX4848937.1"
                     /translation="MTRASTHILRLLRWGRTLARHGALRGIEKDPNTPPQVRRLCRVA
                     RIGTFQPALPDYAGAFQAIGPAAIKLGQALATRPDLVGEEVARNLLSLQDSLPPVPFA
                     AIRREIESSFGKPLEALFVSVEEEPVGAASIAQVHRGVTSEGKTVAIKVLRPGIREKL
                     ASDIETYEWAAAHLEALGGEAARLRPALVIANFKRWTARELDLRREAASASELAELMK
                     GFEGFRVPGVDWDRTNGRVMTIDWIDGTKISDTAALAAQGHELPHLANRLVIGFLTQA
                     IAHGFFHADMHQGNLFVQADGTIAAIDFGIMGRIDRRARMWLAEILYGLTTGNYRRVA
                     EIHFEAQYVPSYHSVDEFATALRAVGEPTRGKPVSELSVGQMLDGLFAITRDFDMQTQ
                     PHLLLLQKTMVMVEGLATQLNPAINMWDVASPYVGSWIRDELGPEAALADRMREDART
                     LLRLPDLVRRIEEKFPARGGAPEPPPLPQVPLIWERRRPSRPWLGHLLAGALGAGALW
                     GAQLLGWL"
     gene            complement(1925..2656)
                     /locus_tag="VFV30_12410"
     CDS             complement(1925..2656)
                     /locus_tag="VFV30_12410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4848938.1"
                     /translation="MNKQVSFGYEEVSPEEKTERVGAVFSSVAKKYDIMNDAMSAGMH
                     RLWKDRFVRRVKPQPGEQILDMAGGTGDIAFRMAARGATITVSDINQDMLDVGIERAM
                     DRGFDSLVWSRQNAEELSFPSRFFDAYTIAFGIRNVTHIDKALAEAYRVLKHGGRFFC
                     LEFSTTEWPGFKEAYDLYSHKLVPRIGEAIAGDADSYRYLIESIRRFPTMPEFESMIR
                     AAGFTRTRVEPILGGLVAIHSGWKV"
     gene            complement(2708..3229)
                     /locus_tag="VFV30_12415"
     CDS             complement(2708..3229)
                     /locus_tag="VFV30_12415"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX4848939.1"
                     /translation="MSASLHAATVPAFIAMLSNIKQWLDQAASEGNEADLIKASLTED
                     MLPLARQIQIASDAAKGAAARLTGTDAPAMADEETTFAELKARCDKTIAYLQTIDPAA
                     YDAGAGREVVITFPNGGGVKFDGHTFLTGFALPNFYFHATTTYAILRARGVKLGKQNF
                     LAHLAPYMFAPPA"
     gene            3339..4154
                     /gene="mutM"
                     /locus_tag="VFV30_12420"
     CDS             3339..4154
                     /gene="mutM"
                     /locus_tag="VFV30_12420"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX4848940.1"
                     /translation="MPELPEVETTVRGLARYLDGERLTRVTPRRADLRRPFPADLVQV
                     LTGARVTGLGRRAKYGLIHTDRGQTMVFHLGMSGRWRIEPDELGKHDHLVLETGGGHT
                     LALCDPRRFGSVDLVASDALDAWPAFAALGPEPLGDALEPTYLKAALAGRAASIKLML
                     LDQRIIAGLGNIYVCEALFRAGIDPRKAAGKVSGPALARLVPEIKAVIAEAIEAGGST
                     LRDYAQPDGELGYFSKRFDVYDRTGQPCTRCGAPVLRLTQGGRSTWHCAKCQK"
     gene            4322..>4391
                     /locus_tag="VFV30_12425"
     CDS             4322..>4391
                     /locus_tag="VFV30_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX4848941.1"
                     /translation="MANTPQAKKRIRRNERRAEINGA"
BASE COUNT          725 a         1449 c         1459 g          758 t
ORIGIN      
        1 cccggcagcg gctgcctcgg cgcggaacgc ggcctcggcc agcggcgaac ggcagatgtt
       61 gccaaggcac acgaacagca ccgccggcgc gctcatttgc cctctccata gcgttccagc
      121 cggaaggcgg ccttcagata gtcttcggcc gcggccttca gcgccggttc cttggcaaag
      181 gcctcggccg gaagcggggc cacccccagc gcttccatca gcgtccacaa tgcaaatctg
      241 cggcccggct cgcgctcgac cccaaagccg atcctcagcc gctccagccc gcgctcgaac
      301 tcggcctcgc tcaggctggc cggatcctcg acctcgaaga agtgcaggat cagcgcatcg
      361 aggtccatcg tctcagagcc agcccagcag ctgcgcgccc cacagcgcgc ccgcgcccag
      421 cgccccggcc agcaagtggc ccagccacgg gcgcgatgga cggcgacgct cccagatcag
      481 cggaacttgc ggcagcggcg ggggttcggg cgcaccgccg cgcgcgggga acttttcctc
      541 gatccgccgg accaggtcgg gcagccgcag cagggtgcgg gcatcctcgc gcatccggtc
      601 ggccagcgcc gcctcggggc ccagttcgtc gcggatccag ctgccgacat agggcgaggc
      661 gacatcccac atgttgatcg cgggattgag ctgggtcgcc agtccttcga ccatcaccat
      721 ggtcttttgc agcagcagca ggtggggctg ggtctgcatg tcgaaatcgc gggtgatcgc
      781 gaacaggccg tcgagcatct gcccgaccga aagctcgctt accggcttgc cgcgggtcgg
      841 ctcgcccacg gcgcgcagcg cggtcgcgaa ttcgtcgacc gaatggtagc tcggcacgta
      901 ctgggcttcg aagtggatct ccgccacgcg gcggtaattg ccggtggtca ggccatagag
      961 aatctcggcc agccacatcc gcgcgcgccg gtcgatccgg cccatgatcc cgaagtcgat
     1021 cgccgcgatc gtgccgtcgg cctgcacgaa caggttgccc tggtgcatgt cggcatggaa
     1081 gaagccgtgc gcgatcgcct gggtcaggaa gccgatcacc agccggttgg ccaggtgcgg
     1141 cagttcatgg ccttgcgcgg ccagcgcggc ggtatcgctg atcttggtgc catcgatcca
     1201 gtcgatggtc atgacccggc cgttggtccg gtcccagtct accccgggca cgcgaaatcc
     1261 ctcgaagccc ttcatcagct cggccagttc cgaggcagat gccgcctcgc ggcgcagatc
     1321 aagctcgcgc gcggtccagc gcttgaaatt ggcgatcacc aatgccgggc gcaggcgcgc
     1381 cgcctcgccc cccaatgcct cgagatgggc ggcggcccat tcgtaggttt cgatgtcgct
     1441 ggcgagcttc tcgcgaatgc cggggcgcag caccttgatc gccacggtct tgccttcgct
     1501 ggtcacgccg cggtggacct gtgcgatcga ggcggcgccg accggctctt cttcaaccga
     1561 gacgaacagc gcctcaagcg gtttgccgaa gctgctttcg atctcgcggc ggatcgcggc
     1621 gaagggaacc ggtggcaggc tgtcctgcag gctcagcaga ttgcgggcaa cttcctcgcc
     1681 caccaggtcg ggccgcgtgg ccagcgcctg gcccagcttg atcgccgccg ggccgatcgc
     1741 ctggaatgcg ccggcatagt cgggcaatgc gggctggaag gtgccgatcc gggccacgcg
     1801 gcacagccgt cttacctgtg ggggcgtgtt gggatccttc tcgatcccgc gcagcgcgcc
     1861 gtgccgggca agtgtgcgtc cccagcgcag caggcgcagg atatgcgtgc tcgcgcgggt
     1921 cacttcaaac tttccatccc gaatggatcg ccaccagacc gcccaggatc ggttcgaccc
     1981 gggtgcgggt gaagccggct gcgcggatca tgctttcgaa ctcgggcatg gtcggaaagc
     2041 gccggatcga ttcgatcaga tagcgataac tgtcggcatc gccggcgatc gcctcgccga
     2101 tcctggggac cagcttgtgc gaatagagat cgtaggcttc cttgaagccg ggccattcag
     2161 tggtcgaaaa ctccaggcag aagaaccgcc cgccatgctt gagcacgcgg tatgcctcgg
     2221 ccagggcctt gtcgatgtgc gtgacgttgc ggatcccgaa ggcgatcgta taggcatcga
     2281 agaagcggct cgggaagctc agctcctcgg cgttctggcg tgaccagacc aggctgtcga
     2341 agccgcggtc catcgccctc tcgatcccga catcgagcat gtcctggttg atgtccgaaa
     2401 cggtgatggt cgcgccgcgc gcggccatgc gaaaggcaat gtcaccggtg ccgccggcca
     2461 tatccaggat ctgttcgccc ggctgcggct tgacccgacg cacgaagcga tccttccaca
     2521 accggtgcat gccggccgac atggcatcgt tcatgatgtc gtacttcttc gccaccgagg
     2581 aaaagaccgc accgacgcgc tcggtctttt cctcgggcga gacttcttcg tagccgaagg
     2641 aaacctgctt gttcatggcc cgcgccttag ccgcaagctg agcgcggtgc cagcaagaag
     2701 aatttgatca ggcaggcggc gcgaacatgt agggcgcgag gtgggcgagg aagttctgct
     2761 tgcccagctt cacgccccgg gcgcgcagga tcgcgtaggt ggtggtggca tggaagtaga
     2821 agttgggcag ggcaaatccg gtcaggaagg tgtgcccatc gaacttgacc ccgccgccat
     2881 tggggaaggt gatcaccacc tcgcgtccgg cgccggcatc gtaggcggcc ggatcgatcg
     2941 tctggaggta ggcgatggtc ttgtcgcagc gggctttcag ttcggcgaag gtggtttcct
     3001 cgtctgccat ggcgggcgcg tcggtcccgg tgagccgcgc ggccgcgccc ttggcagcgt
     3061 cggaggcgat ctggatctgc cgggccagcg gcagcatgtc ttcggtcagg ctggccttga
     3121 taaggtccgc ctcgttgcct tcgctcgcgg cctgatcgag ccattgcttg atattgctga
     3181 gcatggcgat gaacgccggg acggttgcgg cgtgaagcga agcggacatg tggttctccc
     3241 gatgaattgg tgcctggcgg ctctcctatc ctcttggcta ttcagttgca atacgaaacc
     3301 aattgcgact gcggcatggc catggctagg cagtggcgat gcccgagctt cccgaagtcg
     3361 aaaccaccgt gcgcggcctt gcccgttatc tcgatggcga gcgcctgacc cgggtcacgc
     3421 cgcgccgcgc cgatctgcgc cgtccgtttc cggccgatct ggttcaggtc ctgaccgggg
     3481 ccagggtgac ggggctgggg cggcgcgcca aatacggcct gatccacacc gatcgcggcc
     3541 agaccatggt gtttcacctg ggcatgtcgg ggcgctggcg gatcgaaccc gatgagctgg
     3601 gcaagcacga tcacctggtg ctcgaaaccg gcggcggcca caccctggcg ctttgcgatc
     3661 cgcgccgttt cggttcggtc gatctggtcg ccagcgatgc gctcgatgcc tggccggcct
     3721 ttgccgcgct ggggcccgaa ccgctgggcg atgcgctcga accgacctat ctcaaggccg
     3781 cgctggccgg gcgggctgct tcgatcaagc tcatgctgct cgatcagcgg atcattgccg
     3841 ggcttggcaa tatctatgtc tgcgaggcct tgttccgcgc cgggatcgat ccgcgcaagg
     3901 ccgccggcaa ggtttccggc ccggcgctgg cccggctggt gcccgaaatc aaggcagtga
     3961 tcgccgaggc gatcgaggcc gggggatcaa cgctgcgcga ctatgcccag cccgatggcg
     4021 aactgggcta tttctccaag cgcttcgacg tttatgatcg gaccggtcag ccctgcaccc
     4081 gctgcggcgc acccgtactg cgcctgaccc agggcggacg cagcacctgg cactgcgcga
     4141 agtgccaaaa gtagagcctc tacccaaaca ggacaggctc tgggcgcaat tgcttgacga
     4201 ttcgcacctg cagcgttaag ggccgcgctt cttgcgggac cgactccgga cgggccccgc
     4261 gcttttgcac gagattcaac cgctagaggg gctgcacggg cggcccgaac gaaggacgaa
     4321 aatggccaat acgccgcaag ccaagaagcg catccgccgc aacgaacgtc gcgccgaaat
     4381 caacggtgcc c
//
LOCUS       DASUDV010000565         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_660796,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000565 DASUDV010000000
VERSION     DASUDV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660796"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFV30_12430"
     CDS             <1..212
                     /locus_tag="VFV30_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX4848942.1"
                     /translation="EVVKKALDLGAAALILVHNHPSGSPEPSRADIQITGRIAEAARL
                     LGITLHDHVIVGREGHVSLRAKGLV"
     gene            complement(222..485)
                     /locus_tag="VFV30_12435"
     CDS             complement(222..485)
                     /locus_tag="VFV30_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848943.1"
                     /translation="MAKRTEFAFSLAVIGALALAAPAAAKDGQGQPKATTAACSPMEH
                     LGAAIASIWSSNRCRSGGAKKAGDLGQGNSGKAAVKPGGRGAA"
     gene            complement(512..814)
                     /locus_tag="VFV30_12440"
     CDS             complement(512..814)
                     /locus_tag="VFV30_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848944.1"
                     /translation="MVSIYAAYLSVNLIWFYRSEECYRLLAPTWSPTFAFAIQLALVL
                     AYIPREVRELVGDGTARWRRIGVNALYLTLVAILVLTPIAVLQNQERASLEGCTLL"
     gene            complement(824..1168)
                     /locus_tag="VFV30_12445"
     CDS             complement(824..1168)
                     /locus_tag="VFV30_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848945.1"
                     /translation="MDVDGFVTALEKYLREFITHLMRFFRPSVDDEVYDDQEGYNKSV
                     LFALFATAFGAYLWGRYIEGKTNKDIDIFGVMVDNLLEWISLGIVLFLVIRLFRMHAP
                     IVKTAISVLKVF"
     gene            1308..2012
                     /locus_tag="VFV30_12450"
     CDS             1308..2012
                     /locus_tag="VFV30_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX4848946.1"
                     /translation="MCVAAIAWLADPRWRLVAIGNRDEYHARPTDPLEAWPDGRIIAG
                     RDRQAGGTWLGVSEGRFGLVTNLRVPGYPQAELASRGALVTDWLRGQAPRETAAMNPF
                     NLWLAAGERLAFHTNHPTPQQQELPPGIHGLSNGPRGDRWFKTARLEDALARWLADPA
                     PAEALFAALADPTPESTDPEDAFSPVFIRNPVYGTRCSTVVLVDAEGQGRITERRFDP
                     DGAPTGETTRDFAWPV"
     gene            complement(2009..3355)
                     /locus_tag="VFV30_12455"
     CDS             complement(2009..3355)
                     /locus_tag="VFV30_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX4848947.1"
                     /translation="MTTNRPAPAERRFVSKAVEREIERVSALIKDPELRWMFGNCFPN
                     TLDTTVYMGQVEGKADAFVITGDIPALWLRDSAAQLRPYLHLAAGDADLRQLYRGLIR
                     RHARCVLLDPYANAFTRDPASRDGLHWSKNDRTEMKPGVAERKWEVDSLCYVLRLAHD
                     YWRATGDTEPFDATWARAARLIVATFKEQQRFDGPGPYRFQRHTTQPTETTLAGVGVP
                     TKKTGLIHQAFRPSDDACTFPCNIPGNLFAVATLRELAALARGVLRDEALAGEALALA
                     AEVEKAVFAHGVMQLPDGRQVFAYETDGYGNHLFMDDANVPSLLGLAYLRCLPADYPL
                     WRTTADAVWSEANPWFFKGRAAEGIGGPHIGEGQVWPMSIIVRALSSEDEAVIAWSLK
                     TLRNTHAGTGFMHEAFDKDDPAKFTRAWFAWVNGLFGELVVKLAKERPHLLAADYA"
     gene            3572..>4079
                     /locus_tag="VFV30_12460"
     CDS             3572..>4079
                     /locus_tag="VFV30_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4848948.1"
                     /translation="MRAQQPNGIHHVAIMSANMKEQLTFFTQVMGFPLVGLFEMHGVP
                     GGKHAFLKMDEDSFFSVVELAGIGEIPPTLGITHAGTGAGKCAAGTMQHLAFRVSDEA
                     GLIAMRDRIRSHGVPAIGPIGHGFCKSIYFAGPEGLTLEVSYAVSEVDPARWVDPKML
                     AECGIAGAE"
BASE COUNT          676 a         1393 c         1369 g          641 t
ORIGIN      
        1 gcgaagtggt gaagaaggcg ctggacctgg gcgcggcggc gctgatcctg gtccacaacc
       61 acccctccgg atcgcccgaa cccagccgcg ccgacatcca gattacgggc cggatcgccg
      121 aagcggcccg gctgctgggc atcacgctcc acgatcacgt gattgtcggc cgcgaaggcc
      181 acgtcagcct gcgggccaag ggcctggtct gaaccgatcg gctaggcggc gccccgcccg
      241 ccgggtttga cggcggcctt gccgctgttg ccctggccca ggtcaccggc ctttttggcc
      301 ccgcccgaac ggcagcggtt gctggaccag atcgacgcga tcgccgcgcc gagatgctcc
      361 atcggcgaac aggccgccgt ggtggccttg ggctggccct gaccgtcctt ggccgcagcc
      421 ggcgccgcaa gcgcaagcgc gccgatcacc gcaaggctga atgcgaattc ggttcgtttg
      481 gccatgtccc acactcccct ggtgatgtgg atcaaagcag cgtgcagcct tcaaggctgg
      541 cccgctcctg gttctgcaaa acggcgatcg gagttagcac cagaatcgcc accagagtca
      601 aatagagcgc attgacgcca atccgccgcc agcgcgccgt gccatcgccg accagctcgc
      661 gcacctcgcg cgggatatag gccagcacca gcgccagctg gatcgcgaaa gcaaaggtcg
      721 ggctccaggt cggagccagc agccggtagc attcctccga gcggtagaac cagatcaggt
      781 tcaccgagag ataggcggca tagatgctga ccacgtgggc gacctagaag accttgagca
      841 cgctgatcgc cgtcttcacg atcggcgcgt gcatccggaa cagccggatc accaggaaca
      901 gcacgatccc cagcgagatc cattcgagga gattgtcgac catcaccccg aagatatcga
      961 tgtccttgtt cgtcttgccc tcgatatagc ggccccacag ataggcgccg aaagccgtgg
     1021 caaacagggc gaagaggacg ctcttgttgt agccttcctg gtcatcatag acctcgtcgt
     1081 ccaccgaagg acggaagaag cgcatcaggt gggtgatgaa ctcgcgcagg tacttttcca
     1141 gcgcggtgac gaaaccatca acgtccatgc cagcccccca gcctgcaccc ccggccgctt
     1201 cggaccccgc cgcgactcgg ccgggtcctg gccccggatg gcaaggttgc cgcttgccat
     1261 gcggaccgca aggctttcat agacccgggc agagaggagc ttcgatcatg tgcgttgccg
     1321 cgatagcctg gctggccgat ccgcgctggc ggctggtcgc gatcggcaac cgtgatgagt
     1381 accacgcccg gccgaccgac ccgctggagg cctggcccga cggccggatc atcgccgggc
     1441 gggatcggca ggccgggggc acctggctgg gcgtctccga ggggcggttc ggccttgtca
     1501 ccaacctgcg ggtgccgggc tatccccagg ccgaactcgc ctcgcgcggg gcgctggtga
     1561 ccgactggtt gcgcggccag gcgccgcggg aaaccgccgc gatgaacccg ttcaacctgt
     1621 ggctggctgc cggtgaacgg ctggcgtttc acaccaatca ccccacgccg cagcagcagg
     1681 agctgccccc cgggatccac ggcctgtcga acggcccgcg cggcgaccgc tggttcaaga
     1741 ccgcgcggct ggaggatgcc ctggcgcgct ggctggccga tccggccccg gccgaggcgc
     1801 tgtttgccgc gctggccgat ccgacgcctg aatccaccga tccggaggac gccttttcgc
     1861 cggtcttcat ccgcaatccg gtttacggca cgcgctgctc caccgtggtg ctggtcgatg
     1921 ccgagggcca gggccggatc accgagcggc ggttcgatcc ggacggggcg ccgaccggcg
     1981 aaaccacgcg ggactttgcc tggccggtct aggcgtagtc cgccgccagc aggtgcgggc
     2041 gttccttcgc cagcttcacc accagttccc cgaacaggcc gttgacccag gcgaaccacg
     2101 cgcgggtgaa cttggccgga tcgtccttgt cgaaggcttc gtgcatgaag ccggtcccgg
     2161 cgtgggtgtt gcgcagggtc ttcaggctcc aggcgatcac cgcctcgtcc tcgctggaga
     2221 gcgcgcggac gatgatcgac atcggccaga cctgcccctc gccgatgtgc ggcccgccaa
     2281 ttccctcggc cgcgcggccc ttgaagaacc aggggttggc ctcgctccac accgcgtcgg
     2341 cggtggtccg ccacagcgga tagtcggccg gcagacagcg cagataggcc aggcccagca
     2401 ggctggggac attggcatcg tccatgaaca ggtggttgcc gtatccgtcg gtttcgtagg
     2461 cgaagacctg ccgtccatcg ggcagctgca tcaccccgtg ggcaaagacg gccttctcca
     2521 cctcggcggc caaggccaag gcctctccgg ccagggcctc gtcgcgcagc acgccgcggg
     2581 ccagggccgc cagctcgcgc agggtggcca cggcgaagag gttgccgggg atgttgcagg
     2641 ggaaggtgca ggcatcgtcg gacgggcgga aggcctggtg gatcaggccg gtctttttgg
     2701 tcggcacgcc caccccggcc agggtcgttt cggtcggctg ggtggtgtgg cgctgaaagc
     2761 ggtagggacc gggcccatca aagcgctgct gctccttgaa ggtcgccacg atcagccggg
     2821 ccgccctggc ccaggtcgcg tcgaacggct cggtatcgcc ggtcgcgcgc cagtaatcgt
     2881 gggccagccg caggacatag cacagcgaat cgacctccca cttccgctcc gccacgccgg
     2941 gcttcatctc ggtccggtcg ttcttcgacc agtgcagccc gtcccttgaa gccggatcgc
     3001 gggtgaaggc gttggcatag ggatcaagca gcacgcagcg cgcgtggcgg cggatcagcc
     3061 cgcgatagag ctggcgcagg tccgcatccc cggccgcgag gtgcaggtag ggccgcagct
     3121 gcgccgccga atcgcgcagc cacagcgcgg gaatgtcgcc ggtgatgaca aaggcatcgg
     3181 ccttgccctc cacctgcccc atgtagaccg tggtatcgag cgtgttggga aagcagttgc
     3241 cgaacatcca gcgcagttcg gggtccttga tcagcgccga aacccgctcg atctcgcgct
     3301 ccaccgcctt ggagacaaaa cgccgctcgg ccggggcggg acggttggtt gtcatcggct
     3361 tcctctcctg cgcgcgcaaa gcccccggca cggccagcag cgccgcgctg gcggcgagaa
     3421 aactgcggcg gctgatagcg ggcgcgatca ccgggttagt ggtggcctgt cagcggcttg
     3481 ggttcaagcc ccccagcagc ctagttgacg cacttgccaa gcaggccggg tcggctaggc
     3541 tttgccgaaa cgcaccacaa ggacccttcc catgcgcgcc cagcagccca acggcatcca
     3601 ccacgttgcg atcatgtcgg ccaacatgaa ggagcagctg accttcttca cccaggtcat
     3661 gggctttccg ctggtcggcc tgttcgaaat gcacggcgtg cccggcggca agcacgcctt
     3721 cctgaagatg gatgaggaca gcttcttctc ggtggtggag ctggccggga ttggcgagat
     3781 cccccccacg ctggggatca cccacgccgg caccggcgcg ggcaagtgcg cggccgggac
     3841 catgcagcac ctggccttcc gcgtttcgga cgaggccggc ctgatcgcga tgcgcgaccg
     3901 gatccgcagc cacggggttc ccgcaatcgg cccgatcggc cacggcttct gcaagtcgat
     3961 ctatttcgcc gggcccgagg ggctgacgct ggaagtgtcc tatgccgtca gcgaggtcga
     4021 tccggcccgc tgggtcgatc cgaagatgct ggccgaatgc gggatcgcgg gcgcggaag
//
LOCUS       DASUDV010000566        21181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1698947,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000566 DASUDV010000000
VERSION     DASUDV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 21181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21181
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698947"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFV30_12465"
     CDS             <1..321
                     /locus_tag="VFV30_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848949.1"
                     /translation="RYATPKRFAEEMAKGAARSCPGATTSPVQTLQVYGRDAARMRAD
                     CPNNPDTGQPEVFWIVLIAGERDIHARQVAFRRIPNAKDAAWAEGIMAATRLCTQGLA
                     APGC"
     gene            complement(318..668)
                     /locus_tag="VFV30_12470"
     CDS             complement(318..668)
                     /locus_tag="VFV30_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848950.1"
                     /translation="MPAFPSRMFRWAAIYGVIVLTPLYFTPLPPVLGETLLGFIGLAL
                     VFQGVFWLIGGDPVKYRALMPLAVAEKLVFGIPAVALFAQGYPVAPPVAVFAGIDLLL
                     GLGFFVAWRITPRG"
     gene            complement(739..2310)
                     /locus_tag="VFV30_12475"
     CDS             complement(739..2310)
                     /locus_tag="VFV30_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX4848951.1"
                     /translation="MRQAISLLAVAAMLVQGPAVATSSQDPAGSPSAQNSRGMCAPLA
                     PLPRLAAEAGQQGQNYRPRSAPTGGKLRTAREEAPMVDAVPPPPPPPMAPPPPPPPPA
                     PPPPPGSVADASTGEMVVTGSAAPAPVAAKADMGRVEAGTSMPYPGPPPRRPPRPQSG
                     LLTAGEHDDLLNPELYGHYVRTSNLGQEVRGLPYLDTGRVLTVSVKDGAGRPVPFARV
                     EVACADGNRMLLSTLADGTAAFFPDLDRLGSRVKVRAGGQDWREVAIAPGLGGQRVDF
                     TVGRAAQSVRKLDLMLVIDTTGSMGDEIRYLQSELAAILTSLRQRHPGLDLRVGFVFY
                     RDIGDDYVTATFPLTNDLASAQAELRQRFAGGGGDYPEAMDQALVRAAGQPWRPDAVK
                     TMLLVADAPPHDDKFGTTWLAARHLRANRVQIVPVAASGVADKAEYAMRAMAALTQSR
                     YTFLTDDSGIGNPHAPPAIDCYLVTRLDALLRRVIDSQLSGRRIEPEEQEIIREVGQY
                     DNGRCIIPPGALQQR"
     gene            complement(2390..3484)
                     /gene="dprA"
                     /locus_tag="VFV30_12480"
     CDS             complement(2390..3484)
                     /gene="dprA"
                     /locus_tag="VFV30_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX4848952.1"
                     /translation="MAALSQEEAFARIRLLRSPNIGPVSYRQLLRRFGTAAAALEALP
                     DLAARGGAPYRIAPEARIAQEVAAVRASGARYLFHDGADYPALLAELDSAPPILTCRG
                     DGALAARPCVAIVGARNASAAAVKLARQFASDLAEAGLVVVSGLARGIDGAAHRAALE
                     GGTIGVIASGIDIAYPPEHLELQEEVARRGLLLAEQPPGTEPLARHFPSRNRIIAGLA
                     AGTVVIEAAPKSGSLITARLAAEAGREVMAVPGSPLEPRAQGCNQLIRDGAVLVQSAA
                     DVIELVSSFAGTPRSIFRETAPAFEPLPDDLGGDPADIAGLLTTAPVAVDELIRQSGQ
                     SAAAVQLALLELELAGRLVRHAAGRVSLAA"
     gene            complement(3484..4065)
                     /gene="plsY"
                     /locus_tag="VFV30_12485"
     CDS             complement(3484..4065)
                     /gene="plsY"
                     /locus_tag="VFV30_12485"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX4848953.1"
                     /translation="MEWLYALGLGYLLGSVPFGLILTNLFGEGNLRQIGSGNIGATNV
                     LRTGNKGLAAATLLLDGAKGFLAVWLAWQWFPPVAPLAALGAVVGHCFPLWLKFKGGK
                     GVATTLGVTLGLAWPVGLAYAGAWLVMLAVTRISSLSGMTAAVAAPVAAWLLGQPEFV
                     PVLAGIAVLVLWLHRENIARLRAGTEPKVGQKG"
     gene            4147..4944
                     /gene="murI"
                     /locus_tag="VFV30_12490"
     CDS             4147..4944
                     /gene="murI"
                     /locus_tag="VFV30_12490"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX4848954.1"
                     /translation="MDASAPILLFDSGVGGLSVLAELRRALPQAPVIYAADNGGLPYG
                     TKTEAEVAARVCGLLGRMSERYRPRLICIACNTASTIALAMVRDVLNVPIVGTVPAIK
                     PAAEQTRSGVIGLLGTAATIRQPYVDRLAAEFAADKTLLRFAAGELVGAAEAKLRGEA
                     VDPAVYARAAAGLRDQPGGAAIDTVVLACTHFPLVEDELARAFGPGVGFVHGAAGIAR
                     RIVHLTREQDFVRTRPDFALFTKPDADLAALAPALARFDLSAMEVLA"
     gene            4941..5861
                     /locus_tag="VFV30_12495"
     CDS             4941..5861
                     /locus_tag="VFV30_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4848955.1"
                     /translation="MTRRLNRILAALIVLIGLPYYWFLLDNRPGDAQAKPVSIAQLRS
                     LAAEVPGPPPTAAEMELVAFRRLPGTLFAAGTGIKRRLIGVMVWRLPVPGKGPIVIDS
                     GLNAQAAEEMGMEQFDPAAWKRVEANLAAASQIIITHEHADHLGGVVSLGQRALLDKV
                     LFNPQQLPGNRWTDLLTWPAGGVPAPAVTGTAPRAVAPGVVVIPARSHTEGSQMIFVR
                     LADGREFLFAGDIASLAQNWQDLRARSRLVGDVLAPEARGEVFAWLMTIRALKAQAPG
                     LVILPGHDFEWVINPENKTGVAHRFIAPPL"
     gene            5951..7165
                     /gene="hemA"
                     /locus_tag="VFV30_12500"
     CDS             5951..7165
                     /gene="hemA"
                     /locus_tag="VFV30_12500"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX4848956.1"
                     /translation="MNYDQIFDQAIERLHSEGRYRVFIDILRNKGAYPNARCFAGHNG
                     PKDITVWCSNDYLAMGQHPKVIAAMEEALHDVGAGSGGTRNIGGNTHYHVDLEAELAD
                     LHGKEGALLFTSGYVSNDATLSTIAKILPGCVIFSDELNHASMIAGIRNSGAEKRVFR
                     HNDLEHLEELLAAEAPETPKLIAFESVYSMEGDVAPIHAICDLAEKYNALTYIDEVHA
                     VGMYGPRGGGITDRDEAAHRIDIIEGTLGKAFGVMGGYIAADSRIIDVIRSYAPGFIF
                     TTSLSPVLVAGVLAAVRHLKSSSVEREGQQAAAAMLKQLMRDAGLPVMDSTTHIVPLM
                     VGDPVKAKKISDILLAEYGVYVQPINYPTVPRGTERLRFTPGPAHTEAMMRDLTKALV
                     EIWNRLELQLAA"
     gene            7282..8847
                     /locus_tag="VFV30_12505"
     CDS             7282..8847
                     /locus_tag="VFV30_12505"
                     /inference="COORDINATES: protein motif:HMM:NF022468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HEX4848957.1"
                     /translation="MATDWAADVKKYAAKADDAVIAAIVRYCGIALRNRDSALVSFSD
                     PKELDRVRNNYLKKKLGRIEADDVLNAAIAKVGDKMKADRTKNRVTVYYLLADHFKAL
                     GVFAPKAKAAPAKTAAKAPAKAPAKAETKPATKAKAEAKPAAKPAAKTAAKAAGTAAA
                     VAGAAAAAKKPAAKKAAPAKAEAPAKKAAAPAKKAAAPAKKAAAPVKAAAATAAAGGA
                     LGLASAVPAAAKDGAAKVADTAKAAASTATDTAKAAVSAVGDVAKSTADAAGDVAKAA
                     VGAAGDAAKATADAGAAVAGAAGAAAAGAVAAASGAAGKAASAVTGSVQGLTGTSGKD
                     EDSGLGWLWWLLALAAILFLAWWLFLRSPSEEAATTPATTTEAAPAGSDSAAAGATSA
                     LAAAPAEGTVTIPTGAGITTETRDGKPVVKVYFDSGKIDVVPDFGTTAGGLKAWLEAN
                     PGSSLAISGYADPSGNAALNAELAKKRAQAVQAGLVQAGIPEASARLVKPENVTDASV
                     AKDAARRVEVVVQ"
     gene            9117..10037
                     /locus_tag="VFV30_12510"
     CDS             9117..10037
                     /locus_tag="VFV30_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848958.1"
                     /translation="MNNAIKLLVSTAMVATSFAPIFAAPAFAVEANATTDSAMETACT
                     ALAPTNLLHDGTPAGSAEVGNVVATEAAPVVTETEQPGTRAGVGAPTYSNLSIGAGPF
                     RTGGSVNMFGDQIATTRTFAAGSTFLFDRETSVTTSYTWDCTVTVKTETYTPPQPAQG
                     FYINNGTNPSGGGGSCAGLNPNNPNWGQDIGNCTWNETAPAVEGGWTAGPDVVTVVPQ
                     GPTSQTDVTNEYGLTGTDLTPYVQNAGPGDYWYVGKVVICISPKRLPGIWTQQNGYTG
                     DKCTTAWFNVAPWGGGSQDSNGTYISVPAS"
     gene            10127..12733
                     /locus_tag="VFV30_12515"
     CDS             10127..12733
                     /locus_tag="VFV30_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848959.1"
                     /translation="MGTSDLTATRTRLLLTGSVVGLLLAEPQAAAAQTSAPPPPEQTP
                     PPAADGPAAADGTGQDRPSDRNVIVVIGGRTIIAALKDVTVEATYDADAVSAYGVGSV
                     GEVLDEIRNENGDEQPSFLVNGRPVTSPEDIAALPAEAISRIEVLPRGSATKIGGTPG
                     QRAYNVVLRPSLTSTTLTAARTDATEGGWSNTRGEVLFTWIDGQDRLNLSLRGGRSTA
                     LFESERDFVPRTLTTPWSPLGNIIPASGSQIDPALSVLYGQQVTVLALPGGLATPTLA
                     SLLPGANRTNPSGEAAYRTLRGPSQPIDINLSGNKALNEWLSLSLNARLGWTETEGFS
                     GLPTGRFLIPATNPFTPFSAAVSLALNDPSRPLVNRADGNSQSLSATINAQRGPWHGS
                     LTARWDRRENTYLSQATGPLGALGTVAPSINPFAGTLAATIPVTERESTSRNTNSQLV
                     LEVQGPIFALWAGPLSSRATIGITRVDYQASDISGPRALKRTELSSRAGITVPLTSRQ
                     FGFLPALGESEFEFDYGTVDLGSYGTLTRRSLTLNWQPVSWINLVASDNREERPVAPE
                     LLAAPQVITPNVPYFDPVTGQTSDVTLISGGSANLRDEEYRVRSVAVTISPLKNYSLQ
                     FNAEYAVNEVRNQVGALPQPSSAVVAAFPDRFVRDSLGYLTVVDSRSINFAEQRSEVL
                     RVGVRFVIPLTEAGPPVKNAKGQRQRTPPLRLTFNLTHTYVIESTAVIRTGLPEIDLL
                     AGGANGIAGGQLRHSTRAGVALNRGGTGLRIDYDRRGESRLTTGTLAAPDLLRFEPIT
                     TFDLRAFADLGEVFPKAGIPRGTRVTLVVDNLANQRQTVIDRAGTTPQAYQPVRRDPI
                     GRTVMVELRLAF"
     gene            complement(12801..14006)
                     /locus_tag="VFV30_12520"
     CDS             complement(12801..14006)
                     /locus_tag="VFV30_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX4848960.1"
                     /translation="MHSYPDASSVVRALAPDEPVILNRPHAAARAARFFAEKFPGKSL
                     YAVKANPSPDLLKVLWAAGVTHYDVASIAEVRLVRETLPEAVLCYMHPVKPAAAIAEA
                     YRVHGVRTFSLDSHDELAKIVAATAAEDGTPAADLRLCVRLRVSSDYSELSLASKFGI
                     DLADAAPLLQATRQVADWLGVCFHVGSQAMTPFAFVQAMERVRAAIVDASVTVDIVDV
                     GGGFPSIYPGMEPPPLEDYFGAIHRAFESLPVSYSSELWCEPGRALCAEYNSLIVRVE
                     KGRGDELYINDGAYGALYDAAHVGWRFPVKCLTAGRAEVLTPFSFYGPTCDDADYMEG
                     PFLLPADIQAGDYIEIGMLGAYGAAMKTRFNGFGAGQVVDVTDEPMASQYRGDRKDPR
                     AKDNVVSLR"
     gene            complement(14135..15379)
                     /locus_tag="VFV30_12525"
     CDS             complement(14135..15379)
                     /locus_tag="VFV30_12525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4848961.1"
                     /translation="MPDKILDAHTSDPTVMGWMEGLPPPADKRLAWERADHMRFPTHR
                     YAYSHMREFLPTARVARGHGPVWDLPVALRDDIDAVRFTTMDDGRELTWEQSLSVNFT
                     DAVLVLHKGAIVYERYFGVTRRDTPHIAFSVTKSFVGTLAEMLIHEGRLDPAAPMESL
                     IPELKGSGFADATLRQVLDMTTALDFSEEYTDPNSGIGAMSNALGLTPRAPGYAGPGD
                     VYSYLPTIPKAGTHGERFTYRTCNTEVLGWLVARTEGERLDHVLARRIWQPLGMEQDA
                     DFLLDSTAMPFAGGGLNPVLRDLARFGEAMRCEGHGNGAQAIASPVVSAIRSGGRREA
                     FAPAGYTWIPGGAYASQWWLMGGNHGDFSARGIHGQAIWVDPVAEVTIARFGSHPVAA
                     NSAFDPSTLPAFRAVADHLMRG"
     gene            complement(15590..16789)
                     /locus_tag="VFV30_12530"
     CDS             complement(15590..16789)
                     /locus_tag="VFV30_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEX4848962.1"
                     /translation="METRAGDPGAFAHFDLSRVDSPAFVVDAARLRQNLSVLADVRDR
                     AGIKVLAALKAFSMWSVAPIVGEYLDGVCTSGLWEARLASEFYDGEIATYCAAYKPED
                     LDEILRLSDHVIFNSPQQILRCSAIIEAARARGETFDVGLRINPLHPEGEVPRYDPCA
                     PHSRLGFPIDQLTEEHMALVDGIHMHTLCEQDLEPLKRTWDVAFDYLEPYFGQIKWIN
                     LGGGHHITRADYQRDELVEFLKDIQEDTEAEVYLEPGEAVALDAGILVGTVLDLGENG
                     MPVAIVDISATCHMPDVIEAPYRPAMLGELPQLDAEIDEGAGGAVRLGGPSCLAGDVI
                     GDYRFASGPQVGQRFAFLDQAHYSMVKTTTFNGVPLPSIWLWDSETDSLECIKRFGYE
                     DFRDRLS"
     gene            complement(16789..17208)
                     /locus_tag="VFV30_12535"
     CDS             complement(16789..17208)
                     /locus_tag="VFV30_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848963.1"
                     /translation="METSACDPGVLVHSKLTRVLACLTIAAAVLGASPAAQQVKIPIE
                     YRGEWTNEPRYCGVERDNLDSVLTVTAGSVGYFENEWRVKSVQRTGKGIAIRYHPRAD
                     FDMYAPNYLRLSADRQRIYTSDDRRDTGYKRCPRKTR"
     gene            complement(17211..17741)
                     /locus_tag="VFV30_12540"
     CDS             complement(17211..17741)
                     /locus_tag="VFV30_12540"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX4848964.1"
                     /translation="MQVGTTRISIHRGNITQLSVDAIVNAANTSLLGGGGVDGAIHRA
                     AGPDLVHECRLLGGCKPGQAKLTKGYRLPARHIIHTVGPVWRGGTNGEPNVLADCYRN
                     SLSVAIGASLKSIAFSAISTGIYGYPAHQAAKVAIITITEFLTANSGLIDDILLVCFE
                     DKIALSYRDQFPDLED"
     gene            complement(17741..18952)
                     /locus_tag="VFV30_12545"
     CDS             complement(17741..18952)
                     /locus_tag="VFV30_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX4848965.1"
                     /translation="MSKVLVIGAGGVSSVCVHKMAMNAGIFSEIHLASRTKSKCDAIA
                     ASVKERTGVTVHTYEIDAEEVPALTRLIQQIQPKLVVNLALPYQDLPIMDACLAAGVN
                     YLDTANYEPKDQAKFEYSWQWAYQDKFKDAGLMALLGSGFDPGVTSVFAMWLKKHKLK
                     TIRQLDILDCNGGDHGQAFATNFNPEINIREVTAPARHWENGQFVETPAMSKKVAFEF
                     EGVGPKNMYMMYHEELESLAKFIPELERARFWMTFGDAYITHLTVLQNVGMTRIDPVM
                     YQGKEIIPLQFLAAVLPKPETLGPTTKGKTNIGDIATGEALDGSGEKTFYIYNICDHE
                     DAYAETGNQAISYTTGVPAMIGAAMMLTGAWTGEGVFNMEQMDPDPFMDMLNTQGLPW
                     QVKELPGPVEF"
     gene            19000..19977
                     /locus_tag="VFV30_12550"
     CDS             19000..19977
                     /locus_tag="VFV30_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX4848966.1"
                     /translation="MTAQQLRTPTPEGVVRAAGKIADLLPKTPLLEAEIAGQPVWLKA
                     ECLQPIGAFKIRGAWHRLSDLTDEEKARGVVGVSSGNHAQGVAWAARRLGIAATIVMP
                     VDAPRTKLEATRAMGANVVLYDRPGGEDRDEVAQRLIDQSGATLVHAFADPWVIEGQG
                     SAGTEIAAQMVQRDLGGPTRIVCPCGGGGLTAGLALACPDAEIVPVEPEGWDDVRMSL
                     EAGAIVGVPDPSFPTACDALQTPATRPINFAVLKGRVPRGLVVTPAEVRAAQRWAFAN
                     LHLVLEPGGAAALAAALAGKVALDGRTAIVLSGGNTDPEAFAKVLQGND"
     gene            20057..20497
                     /locus_tag="VFV30_12555"
     CDS             20057..20497
                     /locus_tag="VFV30_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4848967.1"
                     /translation="MQAQMLAPAAALVLWSIVMLMWTAATRLPAMKKLGTDLKTAAPG
                     GRGQNLEGVLPDKVQWKSHNYAHLMEQPTIFYPTVIILALLGTNEGTIAIAWAYVALR
                     IAHSLWQATVNTIPVRFTLFILSTVCLLLLAIRAFELTAMGATA"
     gene            20497..20931
                     /locus_tag="VFV30_12560"
     CDS             20497..20931
                     /locus_tag="VFV30_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX4848968.1"
                     /translation="MEQDILKPLAVLAGWTMIMWIWMYATRIPAINKLPKPTEPGADQ
                     GWTGAMLEGLIPREIQWKAHNYNHLHEAPTVFYAVALALAFIGQGNGLNAQIAWAYVA
                     LRIVHSLWQSTVNKVMPRFLLFALSSLCLMALTLHLILAVFH"
     gene            complement(20991..>21181)
                     /locus_tag="VFV30_12565"
     CDS             complement(20991..>21181)
                     /locus_tag="VFV30_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4848969.1"
                     /translation="TMPGVTKSVPFLKGAQLSLRVENLFDSVQTVTDGTGTIPLSYQR
                     SYLDPRGRVITVGFRKAF"
BASE COUNT         3604 a         7322 c         6961 g         3294 t
ORIGIN      
        1 cggtatgcaa cgcccaagcg gtttgccgag gaaatggcca agggtgccgc ccggtcctgc
       61 ccgggagcga cgaccagccc ggtccagacg ctgcaggtgt acggacgcga tgcagcccgg
      121 atgcgggccg attgcccgaa caacccggac acgggccagc cggaagtgtt ctggatcgtg
      181 ctaatcgccg gtgagcgcga cattcatgcc cggcaggttg ccttccgccg gatccccaac
      241 gccaaggacg ccgcctgggc ggaagggatc atggccgcaa cccggctgtg cacccaggga
      301 ctggccgcgc ccggctgtta accgcggggt gttatccgcc aggctacgaa gaagcccaag
      361 cccagcagca gatcgattcc cgcgaagacc gccaccggcg gggcgaccgg atagccctgc
      421 gcgaacagtg ccacggcggg gatcccgaaa accagctttt ccgccaccgc cagcggcatc
      481 agcgcgcggt acttcaccgg atcgccgccg atcagccaga acacgccctg gaacaccagc
      541 gccaggccga tgaaacccag cagggtctcc ccaagcaccg gcggcagcgg cgtgaaatag
      601 agcggggtca gcacgatcac gccatagatc gcggcccagc ggaacatgcg cgatgggaat
      661 gccggcatcg gtccctcctt caacccgaac cggccacccc gcgtgggcgg gatggccggt
      721 ctggagttga aacagggcct agcgctgctg caaggccccc ggcgggatta tgcaccggcc
      781 gttgtcgtac tggccgacct cgcggatgat ctcctgctct tcgggctcga tccggcggcc
      841 cgaaagctgg ctgtcgatca cccgccgcag cagggcatcc agccgggtga cgaggtagca
      901 atcgatcgcc ggcggggcgt gcggattgcc gatcccgctg tcatcggtga ggaaggtgta
      961 ccggctctgc gtcagcgcgg ccatggcccg catcgcgtat tcggccttgt cggccacgcc
     1021 cgaggccgcg accggcacga tctgcacccg gtttgcgcgc aggtggcgag cggccagcca
     1081 ggtggtgccg aacttgtcat cgtgcggcgg agcatcggcg accagcagca tggtcttcac
     1141 cgcatcgggc cgccacggct gcccggcggc gcgaaccagc gcctggtcca tcgcttcggg
     1201 atagtccccc ccgcccccgg cgaagcgctg gcgcagctcg gcctgggccg aagccaggtc
     1261 gttcgtcagc gggaaggtgg cggtgacata gtcgtccccg atgtcgcggt agaacacgaa
     1321 gccgacccgc agatcgaggc cggggtgccg ctggcgcagc gaggtgagga tcgccgccag
     1381 ttccgactgg agatagcgga tctcatcccc catcgaaccg gtggtgtcga tcaccagcat
     1441 caggtccagc ttgcggacag actgcgcagc gcggccgacc gtgaaatcga cccgctggcc
     1501 gccgaggccc ggtgcaatcg ccacctcgcg ccagtcctgc ccgccggccc gcaccttcac
     1561 ccggctgccc agccggtcca gatcggggaa gaacgccgcg gtgccatcgg ccagcgtgct
     1621 gagcagcatc cggttgccat cggcgcaggc cacctcgacc cgggcgaagg gcaccggacg
     1681 gcccgcgccg tccttcaccg acacggtcag cacccgcccg gtatcgagat agggcaggcc
     1741 gcgcacttct tgcccgaggt tggaggtgcg gacgtagtgg ccgtacagtt cggggttgag
     1801 caggtcgtcg tgctcacccg cggtcagcag gccggattgc ggacgcggcg gacggcgcgg
     1861 cggagggccg ggatagggca tggaagtgcc ggcttcgact cggcccatgt cggccttggc
     1921 ggccaccggt gccggtgcgg cgctgccggt aacgaccatc tcgcccgtgc tggcatcagc
     1981 aacgctgccg ggcggcggag gcggtgccgg gggcggcgga ggcggcggag gggccatggg
     2041 cggtggcgga ggcggcggaa ccgcgtccac catgggggct tcctcgcgcg cggtgcgcag
     2101 cttgcccccg gtcggcgccg agcgcggccg gtagttctgg ccctgctggc ccgcctcagc
     2161 cgccaaccgc ggcagcggtg ccagcggcgc gcacatcccg cgcgaattct gggccgaggg
     2221 cgatcccgcc gggtcctgcg atgaggttgc aaccgccggt ccctggacca gcatggccgc
     2281 gacggcgagt agtgagatag cctggcgcat cctgtccctc ctgcgctgtt tctacgccaa
     2341 ggggtgctcc tgcagcgatg aaccgtgcat gaatgtaata aagtatcatt caggccgcaa
     2401 ggctgacccg tccggcggca tggcggacca gccgtccggc cagttccagc tccagcaggg
     2461 ccagttgcac ggcggccgcg ctctgcccgc tttgccggat cagctcgtcc acggcgaccg
     2521 gggcggtggt cagcaggccg gcgatgtcgg ccgggtcgcc gccaaggtca tcgggcaagg
     2581 gttcaaacgc cggagcggtt tcgcggaaga tggatcgggg tgtgccggca aagctgctga
     2641 ccagctcgat cacgtcggcc gccgactgga ccagcaccgc cccgtcgcgg atcagctggt
     2701 tgcagccctg ggcccgcggc tccagcgggg agccgggcac ggccatcacc tcgcgcccgg
     2761 cctctgccgc cagccgggcg gtgatcagcg atccgctttt cggcgcggcc tcgatcacca
     2821 cggtgccggc ggccagcccg gcgatgatcc ggttgcgcga ggggaaatgg cgcgccagcg
     2881 gctccgtccc cggcggctgc tcggccagca gcagcccgcg ccgggctacc tcttcctgca
     2941 gctccaggtg ttcgggcgga taggcgatgt cgatcccgct ggcgatcacc ccgatcgtcc
     3001 cgccctccag cgccgcgcgg tgcgccgccc cgtcgatgcc gcgcgccagg cccgagacga
     3061 ccaccagccc cgcctcggcc agatcggagg cgaactggcg ggccagcttg accgccgcgg
     3121 ccgaggcgtt gcgggcaccc acgatcgcca cacagggccg cgccgccagc gcaccatcgc
     3181 cccggcaggt caggatcgga ggggcgctgt ccagctcggc cagcagggcc ggatagtccg
     3241 cgccgtcgtg aaacagatag cgcgcgccgc tggcccgcac ggccgccacc tcctgcgcga
     3301 tccgggcctc tggcgcgatg cgatagggcg ccccgccccg cgcggcgaga tcgggcagcg
     3361 cctccagcgc ggcggcggcc gtgccgaagc ggcgcagcag ctggcggtag gataccgggc
     3421 cgatattggg cgagcgcagc aggcggatcc gcgcaaaggc ttcctcctgc gagagcgcgg
     3481 ccatcagccc ttctgcccga ccttcggttc ggtcccggcg cgcagccggg cgatgttctc
     3541 acggtgcagc cacagcacca gcacggcgat cccggcaagg accgggacga attccggctg
     3601 gcccagcagc caggccgcga ccggcgcggc gaccgcggcg gtcatgcccg aaagcgagga
     3661 tatccgggtg acggccaaca tgaccagcca ggccccggca taggccagcc cgaccggcca
     3721 ggcgaggcca agcgtgacgc caagcgtggt cgccacgccc ttcccgccct tgaacttcag
     3781 ccacagcgga aagcagtgcc ccaccaccgc gcccagcgcg gccagcgggg caaccggcgg
     3841 gaaccactgc caggccagcc acaccgccaa gaaacccttg gccccatcca gcagcagcgt
     3901 ggccgcggcc aggcccttgt tcccggtgcg cagcacgttg gtggcgccga tgttgcccga
     3961 accgatctgg cgcaggttgc cttcgccaaa caggttggtg aggatcagcc cgaacgggac
     4021 cgagcccagc aggtacccca accccaacgc ataaagccat tccatcgccg caccttccct
     4081 ttcggcccca accgtagcta caaggcggca gcaatcacaa gcctgctttg ggggaatgcc
     4141 acggaaatgg acgcaagcgc gccgatcctg ctgttcgatt cgggcgtcgg cggcctttcg
     4201 gtgctggccg agctgcgccg cgccctgccc caggcgccgg tgatctatgc cgccgacaac
     4261 ggcggcctgc cctatggcac caagaccgag gccgaagtcg ccgcccgcgt ctgcggcctg
     4321 ctcggccgga tgagcgagcg ctaccgcccg cgcctgatct gcatcgcctg caacaccgcc
     4381 agcaccattg ccctcgccat ggttcgcgat gtgctcaacg tgccaattgt cggcacggtc
     4441 ccggcgatca agccggcggc cgagcagacc cgcagcggcg tgatcggcct gcttggcacc
     4501 gcggcgacga tccgccagcc ctatgtcgat aggctcgccg ccgagttcgc cgcggacaag
     4561 accctgctgc gctttgccgc cggcgaactg gttggcgcgg ccgaggccaa gctgcgcgga
     4621 gaggcggtcg atccggcagt ctatgcccgc gccgcggcgg ggctgcgcga ccagccgggc
     4681 ggcgcggcga tcgatacggt ggtgctggcc tgcacccatt tcccgctggt cgaggacgaa
     4741 ctggcccggg cgtttggccc cggcgtcggc ttcgtccacg gcgcggccgg gattgcccgg
     4801 cggatcgtcc acctgacccg cgaacaggac tttgtccgga cccggcccga ctttgccctg
     4861 ttcaccaagc cggacgccga tctggccgcg ctggccccgg cgctggcgcg ctttgacctt
     4921 tccgccatgg aggtgctggc atgacccgca ggctgaatcg gatccttgct gccctgatcg
     4981 tcctgatcgg gctgccctat tactggttcc tgctcgacaa ccggcccggc gatgcccagg
     5041 ccaagccggt ctcgatcgcg caactgcgca gcctggcggc tgaggtaccc ggcccccctc
     5101 ccaccgcggc cgagatggaa ctggtcgcct ttcgccgcct gcccggtacc ctgttcgcgg
     5161 ccggcaccgg gatcaagcgg cggctgatcg gcgtgatggt ctggcgcctg ccggttcccg
     5221 gcaagggccc gatcgtgatc gacagcggcc tcaatgccca ggctgccgaa gaaatgggga
     5281 tggaacagtt cgatccggcc gcgtggaagc gggtcgaagc caacctggcg gcggcatcgc
     5341 agatcatcat cacccacgaa cacgccgatc acctgggcgg agtggtcagc ctgggccagc
     5401 gcgcgctgct ggacaaggtg ctgttcaacc cgcagcagct gcccggcaac cgctggaccg
     5461 acctgctgac ctggcccgcc ggcggcgtgc cggccccggc cgtcaccgga accgcgccgc
     5521 gcgccgttgc gccgggcgtg gtcgtgattc ccgcccgcag ccacaccgag ggatcgcaga
     5581 tgatcttcgt gcggctggcc gacgggcgcg aattcctgtt tgccggggac atcgccagcc
     5641 tggcgcagaa ctggcaggac ctgcgcgccc gctcgcgcct ggtcggcgat gtgctggccc
     5701 ccgaagcccg cggcgaggtc tttgcctggc tgatgacgat ccgcgcgctg aaagcccagg
     5761 ccccggggtt ggtgatcctg cccggccacg atttcgaatg ggtcataaac cccgagaaca
     5821 agaccggcgt ggcccaccgt ttcatcgctc ctccgctttg atccagcgca acgcgttatc
     5881 gctggcattc gggcaaggct tggcgtagag cgcgcgaaaa cgcggcgccc agccggggga
     5941 aacgagtcac gtgaactacg atcagatctt cgaccaggcg atcgagcggc tgcattccga
     6001 aggccgctac cgcgtgttca tcgatatcct gcgcaacaag ggcgcctatc ccaacgcgcg
     6061 ctgctttgcc gggcacaacg ggccgaagga catcacggtg tggtgttcga acgactatct
     6121 ggcgatgggc cagcacccca aggtcatcgc cgccatggaa gaggcgctgc acgatgtcgg
     6181 cgccggttcc ggcggcaccc gcaacatcgg cggcaacacc cattaccacg tcgatctgga
     6241 agccgaactg gccgatctgc atggcaagga aggcgcgctg ctgttcacct cgggctatgt
     6301 ctcgaacgac gcgacgctct ccaccattgc caagattctg cccggctgcg tgatcttctc
     6361 ggacgagctg aaccacgcca gcatgatcgc cgggatccgc aattcgggcg ccgaaaagcg
     6421 cgtgttccgc cacaacgatc tggagcacct cgaagagctg ctcgccgccg aagcgcccga
     6481 aacgcccaag ctgatcgcgt tcgaatcggt ctattcgatg gaaggcgatg tcgccccgat
     6541 ccacgcgatc tgcgacctgg ccgaaaagta caacgccctc acctatatcg acgaagtcca
     6601 cgcggtcggc atgtacggcc cgcgcggcgg cgggatcacc gatcgcgacg aggccgctca
     6661 ccggatcgac atcatcgagg gcacgctggg caaggccttt ggcgtgatgg gcggctatat
     6721 cgcggccgac agccggatca tcgacgtgat ccggtcctat gccccgggct tcatcttcac
     6781 cacctcgctc tcgccggtgc tggtcgcggg cgtgcttgcc gcggtgcgcc acctcaagag
     6841 ctccagcgtg gagcgcgaag gccagcaggc cgccgccgcc atgctcaagc agctgatgcg
     6901 cgatgccggg ctgccggtga tggattcgac cacccatatc gtcccgctga tggtgggcga
     6961 tccggtcaag gccaagaaga tctcggacat cctgctggcc gaatacgggg tctatgttca
     7021 gccgatcaac taccccaccg tcccgcgcgg gacggagcgg ctgcgcttca cccccggccc
     7081 ggcccatacc gaggcgatga tgcgcgatct gaccaaggcc ctggtcgaga tctggaaccg
     7141 gctcgagctg cagctggcgg cctgagccgc cccgcatttc accctagacc acaaggcgga
     7201 atccgttacg actccgggcc gtgccggatt cgccggcatg cctggcgatg atgccgggca
     7261 tggggtcgca gggagtggat tatggcaacc gattgggctg cagacgtcaa gaaatacgcc
     7321 gccaaagccg atgacgcggt gatcgccgcg atcgtccgct attgcggaat cgcgctgcgc
     7381 aaccgcgatt ccgcgctggt ctcgttctcc gatccgaagg agcttgaccg ggtccgcaac
     7441 aactacctca agaagaagct gggccggatc gaagccgacg acgtgctgaa cgccgcgatc
     7501 gccaaggttg gtgacaagat gaaggccgac cggaccaaga accgcgtcac cgtctattac
     7561 ctgctggccg atcacttcaa ggcgctgggc gtcttcgccc ccaaggccaa ggccgccccg
     7621 gccaagaccg ctgcgaaggc tccagccaag gccccggcca aggctgaaac caagcccgca
     7681 accaaggcga aggctgaagc caagccggcc gcaaagccgg cggccaagac cgcagccaag
     7741 gctgctggca cggccgctgc ggttgcgggt gctgctgccg ccgcgaagaa gccggccgca
     7801 aagaaggccg ccccggctaa ggccgaggcc ccagccaaga aggccgccgc tccggcgaag
     7861 aaggccgccg cacccgccaa gaaggcggcg gctccggtga aggccgcggc ggcaaccgcc
     7921 gctgcgggcg gtgccctggg tctcgccagc gcggttcccg ccgctgccaa ggacggcgcg
     7981 gccaaggttg ccgatacggc caaggccgcg gccagcaccg ccaccgatac ggcgaaggct
     8041 gcggtcagcg cggttggcga tgttgccaag agcacggccg acgccgcggg tgacgtggcc
     8101 aaggccgcgg tcggcgcggc cggcgatgcc gcgaaggcga cggccgatgc cggcgcagcc
     8161 gtggccggtg cagccggtgc tgcggccgcc ggtgccgtgg cggcagcttc gggcgcggcg
     8221 ggcaaggcgg cttcggctgt caccggcagc gtccagggcc tgaccggcac ctctggcaag
     8281 gacgaggatt cgggcctggg ctggctgtgg tggctgctgg cgctggctgc catcctgttc
     8341 ctggcctggt ggctgttcct gcgcagcccg agcgaggagg cggcgaccac ccccgccacg
     8401 acgaccgagg ccgccccggc cggcagcgac agcgccgccg ccggtgccac gagcgcactt
     8461 gccgcggctc cggccgaagg cacggtgacg atcccgaccg gggctggcat caccaccgaa
     8521 acccgcgatg gcaagccggt ggtcaaggtc tacttcgaca gcggcaagat cgacgtggtg
     8581 ccggacttcg gcaccaccgc cggcgggctg aaggcctggc tcgaagccaa ccctggctcc
     8641 agcctggcga tttccggcta tgccgatccc agcggcaacg ccgcgctcaa cgcggaactg
     8701 gccaagaagc gcgcccaggc cgtccaggcc gggctggtcc aggccggcat tcccgaggct
     8761 tcggcccggc tggtcaagcc cgagaacgtg accgacgcca gcgtcgccaa ggacgccgca
     8821 cgccgggtcg aagtggtcgt ccagtaaccg ctcgctcccg caccgcggca cgaaggcccc
     8881 gcccggtttc cgggcggggt ttttcgttgc ggcccggccc ctgccgttaa gtcctggcgg
     8941 gcggcctgtc ccccggcggg acgggggaac tttttggtaa atcacgcgtt tacccctacg
     9001 acgaatcgag ttatctgcac gacaagtcga gcaccgactc ggccaaatcc ggtcgcgcgg
     9061 aacagttttc acccatggtc cttgcaacgg gccttggtca ccggagtacc ccaagcatga
     9121 acaacgcaat caagctgctc gtctcgaccg cgatggtcgc caccagcttc gccccgatct
     9181 tcgccgcgcc cgcctttgcg gttgaagcca acgcgacgac cgactcggcc atggaaaccg
     9241 cctgcaccgc gctggccccc accaaccttc tgcacgatgg cactccggcc ggttcggccg
     9301 aagttggcaa cgtcgtcgcc accgaagcgg ccccggtcgt gaccgaaacc gaacagcccg
     9361 gcacccgcgc cggcgttggc gcgccgacct attcgaacct cagcatcggt gccggcccgt
     9421 tccgcaccgg cggcagcgtc aacatgttcg gcgatcagat cgccaccacc cgcacctttg
     9481 cggcgggcag cacctttctg tttgaccgcg aaacctcggt caccaccagc tacacctggg
     9541 actgcacggt gacggtcaag accgaaacct acaccccgcc gcagccggcg cagggcttct
     9601 acatcaacaa cggcaccaac ccttcgggtg gcggcggcag ctgcgccggt ctcaacccga
     9661 acaaccccaa ctggggccag gacatcggca actgcacctg gaacgaaacc gcccccgccg
     9721 tcgaaggcgg ctggacggct ggtcccgacg tggtgaccgt ggttccgcag ggtccgacca
     9781 gccagaccga tgttaccaac gaatacggcc tgaccggcac cgacctcacc ccctatgtgc
     9841 agaacgccgg ccctggcgac tactggtacg tcggcaaggt cgtgatctgc atttcgccca
     9901 agcgcctgcc gggcatctgg acccagcaga acggctacac cggtgacaag tgcaccaccg
     9961 cctggttcaa cgtcgccccc tggggtggcg gttcgcagga cagcaatggc acctacatct
    10021 cggtcccggc cagctgatcc gctgcacccg gtttcgcaat tccccgctgg agcaatccgg
    10081 cggggttttg catatggggc gttgaacagg gaaggatggg gcaaaggtgg gcacttcgga
    10141 tctgacggca acccggacgc ggttgctgct gacgggatcg gtcgtcggcc tgttgctggc
    10201 ggagccacag gccgccgcgg cgcaaacctc tgctcctccc ccgcccgagc agacaccccc
    10261 gcccgccgcc gacgggcccg ccgccgctga cggcacgggc caggaccgcc ccagcgatcg
    10321 caacgtcatc gtagtgattg gcgggcgcac gatcattgcc gcgctcaagg acgtgacggt
    10381 cgaggcgact tacgatgccg atgccgtctc ggcctatggc gtcggctcgg tcggcgaagt
    10441 gctggacgag atccgcaacg agaacggcga cgagcagccc tcgttcctgg tcaacggccg
    10501 tccggtcacc agcccggaag acatcgccgc gttgccggcc gaggcgattt cccggatcga
    10561 agtgctgccc cggggcagcg ccaccaagat cggcggcaca cccggccagc gcgcctacaa
    10621 cgtggtcctg cgcccgtccc tgaccagcac gacgctgact gcggcccgca ccgatgcgac
    10681 cgaaggcggc tggagcaaca cccgcggcga ggtgctgttc acctggatcg acgggcagga
    10741 ccggctcaac ctctcgctcc gcggcggccg ctcgaccgcg ctgttcgaat ccgaacgcga
    10801 tttcgtgccc cgcaccctga ccacgccgtg gtcgccgctt ggcaacatca ttcccgccag
    10861 cggcagccag atcgacccgg cgctgagcgt gctctacggc cagcaggtca ccgtcctggc
    10921 ccttcccggc ggattggcca cgccgaccct ggccagcctg ctccccggcg ccaaccgcac
    10981 caacccatcc ggcgaagcgg cctaccgcac cctgcgcggc ccttcgcagc cgatcgacat
    11041 caacctgtcc ggcaacaagg cgctcaacga gtggctgtcg ctctcgctga acgcccggct
    11101 gggctggacc gagaccgaag ggttcagcgg cctgcccacg ggccgcttcc tgatccccgc
    11161 gaccaacccg ttcacgccgt tctccgccgc ggtcagcctg gccctgaacg atccgtcccg
    11221 tccgctggtc aaccgggctg acggcaacag ccagtcgctc agcgccacga tcaacgccca
    11281 gcgcggccct tggcacggct cgctgaccgc ccggtgggac cggcgcgaga acacctacct
    11341 gagccaggcg accgggccgc ttggcgcgct gggcactgtc gcgccttcga tcaacccctt
    11401 tgccggaacc ctggccgcca ccatcccggt caccgaacgc gaaagcacca gtcggaacac
    11461 caacagccag ctggttcttg aggttcaggg gccgattttc gcgctgtggg ccggcccgct
    11521 cagctcgcgc gcgaccatcg ggatcacccg ggtcgactat caggccagcg acatttccgg
    11581 cccgcgcgcg ctcaagcgga ccgagctgtc ctcgcgcgcg gggatcaccg tgccgctgac
    11641 cagccgccag ttcggctttc tgcccgcgct gggggaaagc gagttcgaat tcgattacgg
    11701 cacggtcgat ctgggcagct atggcacgct gacccgccgc tcgctgaccc tgaactggca
    11761 gccggtgtcc tggatcaacc tggtggccag cgacaaccgc gaggaacgcc cggttgcgcc
    11821 cgaactgctg gccgcgccgc aagtgatcac gccgaacgtg ccctatttcg atccggtgac
    11881 cgggcagacc agcgatgtca cgctgatcag cggcggttcc gcgaacctgc gggacgagga
    11941 gtaccgggtc cgctcggtcg ccgtcacgat cagcccgctc aagaattaca gcctgcagtt
    12001 caacgccgaa tacgcggtga acgaagtgcg caaccaggtg ggcgccctgc cccagcccag
    12061 ctcggccgtg gtcgccgcct ttccggaccg gttcgtgcgc gacagcctgg gctatctgac
    12121 cgtggtcgac agccgctcga tcaactttgc cgaacagcgc agcgaggttc tgcgcgtggg
    12181 cgtgcggttc gtgattccgc tgactgaggc cggcccgccg gtcaagaacg ccaagggcca
    12241 gcgccagcgc acgccgccgc tgcggctgac cttcaacctg acccacacct atgtgatcga
    12301 aagcaccgcg gtgatccgca ccggcctgcc ggagatcgat ctgctggccg gcggggccaa
    12361 cgggattgcc ggcgggcagc tgcgccattc gacccgggcc ggcgtggcgc tgaaccgcgg
    12421 cggcaccggg ctgcggatcg attacgaccg ccgcggggaa agccgcctga ccaccggcac
    12481 gctggccgcg ccggacctgc ttcggttcga acccatcacg accttcgatc tgcgcgcctt
    12541 tgccgatctg ggcgaggtgt tccccaaagc cggcatcccg cggggcaccc gcgtgaccct
    12601 ggtggtcgac aacctggcca accagcgcca gaccgtgatc gaccgcgcgg gcacgacgcc
    12661 ccaggcctat cagccggtgc ggcgcgatcc gattgggcgg accgtgatgg tcgagctgcg
    12721 tctggccttc tgagccgccg cccaaacgaa aaagggcggc accgtagcgc cgccctttgt
    12781 tgggttccgg tggagtccga tcagcgcagg ctgacgacgt tgtccttggc gcggggatcc
    12841 ttgcggtcac cgcggtactg gctggccatc ggctcgtcgg tcacgtcgac cacctggccc
    12901 gcgccaaagc cgttgaagcg ggtcttcatc gccgcgccat aggcaccgag catgccgatc
    12961 tcgatatagt caccggcctg gatatcggcc ggcagcagga aggggccttc catgtagtcg
    13021 gcatcgtcgc aggtcgggcc atagaacgag aacggggtca gcacttcggc ccggcccgcg
    13081 gtcaggcact tgaccgggaa ccgccagccg acatgggccg catcgtacag cgcgccatag
    13141 gcgccgtcgt tgatgtagag ctcgtcgccc cgaccctttt cgacccgcac gatcagcgag
    13201 ttgtattccg cgcagagcgc gcggccgggt tcgcaccaca gttccgacga ataggacacc
    13261 ggcagcgatt cgaacgcgcg gtggatcgcg ccgaagtagt cctccagcgg cggcggttcc
    13321 atgcccgggt agatcgaggg gaaacccccg ccgacatcga cgatatcgac cgtgaccgag
    13381 gcatcgacaa tcgcggcgcg gacccgctcc atcgcctgga cgaaggcaaa cggggtcatc
    13441 gcctggctgc cgacgtggaa gcatacgccc agccagtccg cgacctggcg ggtcgcctgc
    13501 agcagcggcg cggcatcggc caggtcgatc ccgaacttgg aagccaggct cagctccgaa
    13561 tagtcggacg agacgcgcag ccgcacgcac aggcgcaggt cggccgccgg ggttccgtct
    13621 tcggcggccg tggcggcaac gattttggcc agctcgtcat ggctgtcgag gctgaaggtg
    13681 cgcacgccgt ggacgcggta ggcctcggca atcgcggccg cgggcttcac cgggtgcata
    13741 tagcacagca cggcttcggg cagggtttcc cgcaccagcc gcacttcggc gatcgaggcg
    13801 acgtcgtaat gcgtcacccc ggcggcccag aggaccttca gcaggtcggg cgacgggttc
    13861 gctttgaccg catagagcga cttgcccgga aacttctcgg caaagaagcg ggcggcgcgt
    13921 gcagcggcat gcgggcggtt gaggattacg ggttcgtccg gcgcgagggc gcgaactact
    13981 gacgacgcat cgggatagct gtgcaactca agggaccccc aataggcctt ccggccaggt
    14041 aacacgaaga cgaggctgcc ttgcggttat tggaagtccc cttggggcag cggggggtcg
    14101 ctataaagat tcgcgcctta atcgcaagtg aaaatcaccc ccgcatcagg tggtccgcga
    14161 cggcccggaa ggccggcagg gtcgaggggt cgaaggccga atttgccgcc accggatgcg
    14221 agccgaaccg ggcgatcgtg acctcggcca cgggatcgac ccagatcgcc tgaccgtgaa
    14281 tcccgcgggc cgaaaaatcg ccgtgatttc cgcccattag ccaccactgg ctggcatagg
    14341 caccgcccgg aatccaagtg tatccggccg gggcaaaggc ctcgcgccgg ccgcccgaac
    14401 ggatcgcgga gaccaccgga ctggcgatcg cctgggcgcc gttgccgtgc ccttcgcagc
    14461 gcatcgcttc gccgaatcgg gccaggtcgc gcagcaccgg attgagcccg cccccggcaa
    14521 agggcatcgc ggtcgaatcg agcaggaaat cggcgtcctg ttccatcccc agcggctgcc
    14581 agattcgccg cgccaggaca tggtcgagtc gctcgccctc ggtccgcgcg acgagccagc
    14641 cgagcacttc ggtgttgcag gtgcggtaag tgaaacgctc tccgtgcgtg cccgccttgg
    14701 ggatcgtcgg caggtaggaa tagacatccc ccggcccggc atagcccggc gcgcgcggag
    14761 tcaggcccag ggcgttgctc atcgcgccga tgcccgaatt ggggtcggtg tactcctcgc
    14821 tgaaatccag cgcggtggtc atgtccagca cctggcgcag agtggcgtcg gcaaagccgc
    14881 tgcccttcag ttcggggatc aggctttcca tcggcgcggc gggatcgagc cggccttcgt
    14941 ggatcagcat ttccgccagg gtgccaacaa agctcttggt caccgaaaag gcgatgtgcg
    15001 gcgtatcccg gcgggtgacg ccgaagtagc gctcatagac gatcgcgccc ttgtgcagca
    15061 ccagcacggc atcggtgaag ttgacgctga gcgactgctc ccaggtcagt tcgcgcccgt
    15121 cgtccatggt ggtgaagcgc accgcgtcga tatcgtcccg cagcgccacc ggcaggtccc
    15181 agaccggccc gtgcccgcgc gcgacccgcg cggtcggcag gaattcgcgc atgtgcgaat
    15241 aggcatagcg gtgggtcgga aagcgcatgt gatccgcccg ctcccacgcc aggcgcttgt
    15301 cggccggcgg cggcagcccc tccatccagc ccatgacagt gggatcggag gtgtgggcat
    15361 caaggatctt gtcgggcatg gctctctcct gtttggtgga gagactaacc atatggagcc
    15421 gttgcgccag atgttgctca cgcaaagacg caaaggcgct aagatcttgc gccgagccgc
    15481 aggctcagtc gctgtccaaa gccctgcaac cgaaagaacg aatgcggcag cggcggctta
    15541 gccgctggcg gcacatcttc gcgcctttgc gtctttgcgt gagcttcaat cagctcagcc
    15601 ggtcgcggaa gtcctcgtag ccgaagcgct tgatgcactc caaactgtcg gtctcgctgt
    15661 cccacagcca gatcgacggc agcggtacac cgttgaaggt ggtggtcttg accatcgagt
    15721 aatgcgcctg gtcgaggaag gcgaaacgct ggccgacctg cggcccgctg gcaaagcggt
    15781 aatcgccgat cacgtcgccg gccaggcagc tcggcccgcc gaggcgcacc gccccgcccg
    15841 cgccttcgtc gatctcggcg tcgagctgcg gcagctcgcc cagcatcgcc gggcggtagg
    15901 gcgcttcgat cacatcgggc atgtggcacg tggccgagat atcgacgatc gccactggca
    15961 tcccgttctc gcccagatcg agcacggtac cgaccaggat cccggcatcg agcgccaccg
    16021 cctcgcccgg ctcgagatag acctcagcct cggtgtcctc ctggatgtcc ttcaggaatt
    16081 cgaccagctc gtcgcgctgg taatcggcgc gggtgatgtg gtgcccgccg cccaggttga
    16141 tccacttgat ctggccgaag tacggctcaa gatagtcaaa ggcgacgtcc caggtgcgct
    16201 tcagcggctc cagatcctgt tcgcacaggg tgtgcatgtg gatcccgtcg accagcgcca
    16261 tgtgctcttc ggtcagctgg tcgatcggaa agccgaggcg cgaatgcggg gcgcagggat
    16321 cataccgcgg cacttcacct tcggggtgca agggattgat ccgcaacccg acatcgaagg
    16381 tttcgccgcg cgcccgggcg gcttcgatga tcgcggagca gcgcaggatc tgctgcggcg
    16441 aattgaagat cacgtggtcc gacaggcgca ggatctcgtc cagatcctcg ggcttgtagg
    16501 ccgcgcaata ggttgcgatc tcgccgtcat agaactcgct cgcaagccgc gcttcccaca
    16561 ggccggaggt gcagaccccg tcgaggtatt cgccgacaat cggcgccacc gaccacatcg
    16621 aaaaggcctt cagcgcggca aggaccttga tccccgcccg gtcgcgcaca tcggccagca
    16681 ccgacaggtt ctgccgcagc cgcgcggcat cgaccacgaa ggcggggcta tcgacccggc
    16741 tgaggtcgaa gtgggcaaaa gcccccggat cgccggctct ggtttccatt atcgcgtttt
    16801 ccttgggcag cgcttgtatc cggtgtcgcg ccggtcatcg ctggtgtaga tgcgctgccg
    16861 gtcggccgac aggcgcaggt aattgggcgc gtacatatcg aaatcggcgc gcgggtgata
    16921 gcggatcgca atccccttgc cggtccgctg caccgacttc acccgccatt cgttctcgaa
    16981 atagccaacc gagccggccg tcaccgtcag aacggaatcg agattgtccc tctccacccc
    17041 gcaatagcgc ggctcgttgg tccattcgcc gcgatactcg attggaattt tgacttgctg
    17101 cgcagccggg ctggcaccca gaacggccgc cgcgatggtg agacaggcca agacgcgtgt
    17161 gagtttagaa tggacgagaa cacccggatc gcacgcgctg gtttccacac tcagtcctcc
    17221 aaatcgggaa attgatcgcg ataagacagt gcgatcttgt cttcgaaaca aaccagcaga
    17281 atatcgtcga tcagtcctga attggccgtc aaaaactcag tgatcgtaat gatcgcaacc
    17341 ttagctgcct gatgtgctgg atagccatag ataccagtcg aaattgcgga gaatgcaatt
    17401 gacttcaggc tggctccgat ggctaccgac aagctgttgc ggtagcaatc tgctagcaca
    17461 ttaggctcac catttgtgcc gccgcgccaa acggggccga ccgtatggat tatgtgtcgt
    17521 gcaggcagcc gataaccctt cgtgagcttt gcctgacccg gcttgcatcc ccccagcaga
    17581 cggcattcgt gcaccagatc aggcccagcg gcacgatgga tcgctccatc gacgcccccg
    17641 ccgcccaaca gcgaggtatt ggcagcgttg actatcgcat cgacggaaag ctgagtgata
    17701 ttgccgcgat ggattgagat gcgggtcgtg ccgacctgca tcaaaactcc accggccccg
    17761 gcagttcctt cacctgccac ggcaggccct gggtattcag catgtccatg aacggatcgg
    17821 gatccatctg ctccatgttg aacacgccct cgcccgtcca ggcgccggtc aacatcatcg
    17881 ccgcgccgat catcgccgga acgccggtgg tgtagctgat cgcctggttg ccggtttcgg
    17941 cataggcgtc ttcgtgatcg cagatgttgt agatgtagaa ggtcttctcg ccgctgccat
    18001 cgagcgcctc gccggtcgcg atgtcgccga tgttggtctt gcccttggtc gtcgggccca
    18061 gcgtttcggg cttgggcagc acggcggcga ggaactgcag cgggatgatt tccttgccct
    18121 ggtacatcac cgggtcgatc cgggtcatgc cgacgttctg cagcacggtg aggtgcgtga
    18181 tataggcatc gccgaaggtc atccagaagc gggcgcgctc cagctcgggg atgaacttgg
    18241 ccaggctttc cagctcctcg tggtacatca tgtacatgtt cttggggccg acgccctcaa
    18301 actcgaaggc caccttcttg ctcatcgccg gggtttcgac gaactggccg ttctcccagt
    18361 ggcgggccgg tgcggtcact tcacggatgt tgatttccgg attgaagttg gtggcaaagg
    18421 cctggccgtg atcgccgccg ttgcagtcca gaatgtcgag ctggcggatg gtcttgagct
    18481 tgtgcttctt cagccacatc gcaaagacgc tggtcacgcc cggatcgaag cccgagccga
    18541 gaagcgccat caggccggca tccttgaatt tgtcctgata ggcccactgc cacgagtatt
    18601 cgaacttggc ctgatccttc ggctcgtagt tcgcggtgtc gagataattg accccggcgg
    18661 ccagacaggc gtccatgatc ggcaggtcct ggtacggcag cgccagattg acgaccagct
    18721 tcggctggat ctgctggatc agccgggtca gcgcgggcac ttcctcggcg tcgatctcat
    18781 aggtgtggac ggtaacgccg gtgcgctcct tgaccgaggc ggcgatcgca tcgcactttg
    18841 acttggtccg gctggcgagg tggatctcgc tgaaaatgcc cgcgttcatc gccattttgt
    18901 ggacgcagac cgagctgacg ccgcccgcac cgatcaccag aaccttgctc actgctcgaa
    18961 tctccttgcc gcagacggac gggcatatag atttgtttca tgaccgcaca acaattgcga
    19021 actccgactc ccgaaggggt agtccgcgcg gccggcaaga tagccgatct gctgccgaaa
    19081 acacccctgc ttgaagcaga gatcgcaggc cagccggtct ggctgaaggc cgagtgcctg
    19141 cagccgatag gcgccttcaa gatccggggg gcctggcacc ggctgagcga cctgacggac
    19201 gaagagaagg cgcgcggcgt ggtcggcgtg tccagcggca accatgcgca aggcgtggcc
    19261 tgggcggcgc ggcggctggg gatcgcggca accatcgtca tgccggtcga tgcccctcgc
    19321 accaagctgg aagcaacccg ggcaatgggc gccaatgtcg tgctctatga ccgccctggc
    19381 ggcgaggacc gtgacgaagt ggcccagcgc ctgatcgacc agagcggcgc gaccctggtt
    19441 cacgcctttg ccgatccctg ggtgatcgag ggccagggca gcgccgggac cgagattgcc
    19501 gcgcagatgg tccagcgcga cctcggcggc cccacccgga tcgtctgccc ctgcggcggc
    19561 ggcgggctga ccgcgggcct tgccctcgcc tgccccgatg cggaaatcgt gccggtggag
    19621 cccgaaggct gggacgacgt gcggatgagc ctggaggccg gggcaatcgt cggcgtgccc
    19681 gatccgtcct tccccaccgc ctgcgatgcg ctgcagaccc cggcaacccg cccgatcaac
    19741 tttgccgtgc tcaagggccg ggttccgcgc gggctggtgg tgacgccggc cgaagtccgc
    19801 gcggcgcagc gctgggcctt tgccaacctg cacctggtgc tggagcccgg cggcgcggcg
    19861 gcgctggcgg cggccctggc cggcaaggtg gcgctggacg ggcgcaccgc gattgtcctg
    19921 tcgggcggca acaccgatcc cgaagccttc gccaaggtcc tgcaaggcaa cgattagttg
    19981 catttgacaa tcggtttcaa caggcacacc ctgccgccct cggttcagac gagccaaggg
    20041 ggagaaacca atgaccgtgc aagcgcaaat gctcgcgccg gcggccgcgc tggtgctgtg
    20101 gtccatcgtc atgctgatgt ggaccgcggc aacccgcctg cctgcgatga agaagctggg
    20161 tactgatctg aaaaccgccg cgcccggcgg ccgcggccag aacctggaag gcgtgctgcc
    20221 cgacaaggtg cagtggaaat cgcacaacta cgcccacctg atggaacagc cgacgatctt
    20281 ctatccgacc gtcatcatcc tggcgctgct ggggacgaac gagggaacga tcgccatcgc
    20341 ctgggcctat gtcgcgctgc ggattgccca ttcgctgtgg caggccaccg tgaacaccat
    20401 tccggtgcgc ttcacgctgt tcatcctttc gaccgtgtgc ctgctgctgc tggccatccg
    20461 tgcctttgaa ctcactgcca tgggagcgac tgcataatgg aacaggacat cctcaaaccg
    20521 ctggccgtgc tggccggctg gaccatgatc atgtggatct ggatgtatgc cacccggatc
    20581 cccgccatca acaagctgcc caagccgacc gagcccggcg ccgaccaggg ctggaccggg
    20641 gccatgctgg aagggctgat cccgcgcgaa atccagtgga aggcacacaa ctacaaccac
    20701 ctgcacgaag cgccgacggt gttctatgcc gtggcgctgg cgctggcctt catcggccag
    20761 ggcaacggcc tcaacgcgca gattgcctgg gcctatgtcg ccttgcggat cgtccactcg
    20821 ctctggcagt cgaccgtcaa caaggtgatg ccgcgcttcc tgctgttcgc cctgtccagc
    20881 ctgtgcctga tggcgctgac gctgcacctg atcctggcgg tgttccacta agccggatcc
    20941 ctgaaaagcc cccggccggc ggaccggccg gggcgctttt gcaagtgcct ttagaaggcc
    21001 ttgcggaaac cgaccgtaat cacccgtccg cgcggatcga gatagctgcg ctggtacgac
    21061 agtgggatcg tgccggtccc gtcggtgacg gtctggaccg aatcgaacag gttctcgacc
    21121 cgcagcgaaa gctgcgcgcc cttcaggaac ggcaccgact tggtcacgcc cggcatcgtc
    21181 g
//
LOCUS       DASUDV010000567         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_439493,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000567 DASUDV010000000
VERSION     DASUDV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439493"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..721)
                     /locus_tag="VFV30_12570"
     CDS             complement(143..721)
                     /locus_tag="VFV30_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848970.1"
                     /translation="MIEAGPVELATLVSDEAFAAAAAKAREECAEEGGGRLQRALTGL
                     AGPKIAAAIGEKFRAIDLLPLFIRGWGQSPELAAEADKSAKSSEPHRVRLGKLEQDLD
                     LYPILGLSAWGLTASPIQFTLTLKAEFEAIEVTMEQGYVVQIGGGICRLAAVLKLGQF
                     TFPSGFDPVEWKVGKGRAFERPGMPIFPQKAA"
     gene            complement(718..1509)
                     /locus_tag="VFV30_12575"
     CDS             complement(718..1509)
                     /locus_tag="VFV30_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848971.1"
                     /translation="MAGEKARAGLLTAAAAGLVWCAPALAQTGTRPTASSAPATDPAS
                     QQQAQPAIKVTPQQVIRAVTTLIQKKPPAPAPTTSPAPAQTAAPAPAGTPTAAPTLVP
                     TAAPAAATGTAPRPAATRPAASLRPAPAPAPRDDAVPAEPEPAPPPSAPPPPAPVDPV
                     PEPVEPSPPVAEVIPEPPPAPAPFPWAWLAVLAAAAAGGGYGLRRWFWPKLGIDCHID
                     VGPTVRIAGSSPLVEAPELRIEVRIEPGQPSPPRGVTILAAGGQP"
     gene            1584..3131
                     /locus_tag="VFV30_12580"
     CDS             1584..3131
                     /locus_tag="VFV30_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX4848972.1"
                     /translation="MNKPNFKHALSVTRTEDFAQWYQEVISEAEMAEESGVRGCMVIK
                     PWGYGIWERIQKLMDARIKAAGVDNCYFPLFIPLSYFAKEAEHVDGFAKEMAVVTHHR
                     LIQDGKGGLVPDPEAKLEEPLIVRPTSETVIGQAMARWVQSWRDLPLLTNQWANVVRW
                     EMRTRMFLRTSEFLWQEGHTAHADRDDAMAETLRALEMYREHAEDDLAMPVIAGEKPE
                     NERFPGAVATYSIEAMMQDGKALQAGTSHYLGTGFAEAAGIKYQDREGTQQLCHTTSW
                     GVSTRLIGGVIMTHGDDDGLRVPPRIAPHQIVILPMLREDDGDAALLAYCEDLRRALA
                     AQTALGEPVRVLLDKRAGKATQKRWGWVKKGVPLILEIGGRDAAGGQVSAVRRDRLYR
                     EDGKVNFVGQAKDDFLAAAAGELEDIQHSLHAEATARRDAQIHRGITDLAGIEAFFAE
                     GQKYPGWVEISWSRPTGAVLEAVVAKLKALKLTIRNTPMDGNPVTGTCPFTGEPAVER
                     IYVARAY"
     gene            3215..3664
                     /locus_tag="VFV30_12585"
     CDS             3215..3664
                     /locus_tag="VFV30_12585"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEX4848973.1"
                     /translation="MRNALALAFAFAASATAAQADEWRLVSENEGRPGIAMYIDQASV
                     VRTGHTVTGWVMTVREYDPKAADWTKSLIFRQIDCDRNIAQMMQSKFYGPKGNLLEDT
                     KVPANWQNIVDGSMLEGVALVMCGKDGYASNVVPDPMADARARFAKH"
BASE COUNT          623 a         1275 c         1303 g          598 t
ORIGIN      
        1 ccgccgtccc gaagggattt ggccagaatg gcccagggct tcgtcaggaa gtcttgaaat
       61 atcgacatat tcctgcgcct tccttcctgc ccctgagcca ttctgactca aaccgcgctg
      121 gtgcggttaa ggggtcctga ccctaggccg ccttctgcgg aaagatcggc atgcccgggc
      181 gctcgaatgc gcgtcccttg ccgaccttcc attcaaccgg atcgaacccg ctggggaagg
      241 tgaattgccc cagcttcagc acggccgcca ggcggcagat gccgccgccg atctgcacca
      301 catagccctg ttccatcgtc acttcgatcg cttcgaactc ggccttcagg gtcagggtga
      361 actggatcgg gctggcggtc agcccccagg ctgacaggcc gaggatcggg tagagatcaa
      421 ggtcctgctc cagcttgccc aggcgcacgc ggtgcggctc ggaactctta gcgctcttgt
      481 ccgcctcggc ggccagttcc ggtgactggc cccacccgcg aatgaacagc ggcagcaggt
      541 ctattgcccg gaacttctcg ccgatggcgg ccgcaatctt cgggccggcc aggccggtca
      601 gcgcacgttg caaccgcccg ccgccctctt cggcgcactc ttcgcgcgcc ttcgccgcgg
      661 cggcggcgaa ggcttcgtcc gataccagcg tggcaagttc gaccgggccg gcttcaatca
      721 tggctgtcct cctgcggcca gaatggtcac cccgcgtggg ggactgggct ggccgggttc
      781 gatccggact tcgatccgca attcgggcgc ctcgacaagc gggcttgatc cggcgatgcg
      841 gacggttggg cccacgtcaa tgtggcaatc gatgcccagc ttgggccaga accaccgccg
      901 cagcccataa ccgccgccgg ccgccgccgc agccagcacg gccagccagg cccaggggaa
      961 cggggcgggc gccgggggcg gttccggaat gacctctgca accgggggac tgggttcaac
     1021 cggttccggg acagggtcga ccggcgcagg aggcggtgga gcggacggag gcggggccgg
     1081 ttcgggctcc gccggcacgg catcgtcgcg cggcgccggg gccggggcgg ggcggagcga
     1141 ggcagccggg cgggtcgccg ccgggcgcgg cgcggttccg gttgccgcgg caggcgccgc
     1201 cgtcgggacc agagtgggcg cggcggtggg cgtgccggcc ggggcggggg cagcggtctg
     1261 ggccggcgcg gggctggttg tcggcgcggg cgcgggaggc ttcttctgaa tcagcgtggt
     1321 gaccgcgcgg atcacctgct gcggcgttac cttgatcgcg ggctgggcct gctgctgcga
     1381 tgctggatcg gttgcggggg cggagctggc ggttggccgg gttcccgttt gcgccaaagc
     1441 cggcgcgcac cagaccagtc ccgccgcagc agcggtcagc aatcccgccc tggctttttc
     1501 ccccgccatg gccaaggcat aacacggctt ggcccgggcg caaaacttgc cccgtccgtg
     1561 ccaagccgct atcgcgccgc gccatgaaca agcccaattt caagcatgcc ctgtccgtga
     1621 cccgcacaga ggatttcgcc cagtggtatc aagaggtcat ctccgaagcc gagatggccg
     1681 aggaaagcgg cgtgcgcggc tgcatggtga tcaagccgtg gggctatggc atctgggaac
     1741 gcatccagaa gctgatggac gcgcggatca aggcggccgg ggtcgacaac tgctacttcc
     1801 cgctgttcat cccgctgtcc tacttcgcca aggaggccga gcacgttgac ggctttgcca
     1861 aggaaatggc ggtcgtcacc caccaccgcc tgatccagga cggcaagggc gggctggtcc
     1921 ccgatcccga ggccaagctg gaagagccgc tgatcgtgcg ccccacgtcg gaaacggtca
     1981 tcgggcaggc gatggcgcgc tgggtgcagt cgtggcgcga tctgccgctg ctgaccaacc
     2041 agtgggccaa cgtggtccgc tgggaaatgc gcacccggat gttcctgcgc accagcgagt
     2101 tcctgtggca ggaagggcat accgcccatg ccgaccgcga cgacgccatg gccgaaaccc
     2161 tgcgcgcgct ggaaatgtac cgcgagcacg ccgaggacga tctggccatg ccggtgatcg
     2221 ccggcgaaaa gcctgagaac gagcgcttcc cgggtgccgt ggcgacctat tcgatcgagg
     2281 ccatgatgca ggacggcaag gcgctgcagg ccggcacctc gcactatctc ggcaccggtt
     2341 tcgccgaagc cgccgggatc aagtaccagg accgcgaagg cacccagcag ctgtgccaca
     2401 ccaccagctg gggggtttcg acccggctga tcggcggcgt gatcatgacc cacggcgacg
     2461 atgacgggct gcgcgtgccg ccccggatcg ccccgcacca gatcgtgatc ctgccgatgc
     2521 tgcgcgagga cgatggcgat gcggcgctgc tggcctattg cgaggatctg cgccgcgcgc
     2581 tggccgctca gaccgcgctg ggcgagccgg tccgcgtgct gctcgacaag cgggccggca
     2641 aggccacgca aaagcgctgg ggctgggtca agaagggcgt gcccctgatc ctcgagatcg
     2701 gcgggcgcga tgctgccggc gggcaggttt cggccgtgcg ccgcgaccgg ctctaccgcg
     2761 aggacggcaa ggtcaacttc gtcggccagg ccaaggacga tttccttgcc gcggcggcgg
     2821 gtgagctgga ggacatccag cactcgctcc atgccgaggc gaccgcgcgt cgcgacgcgc
     2881 agatccaccg cgggatcacc gatctggccg ggatcgaggc cttctttgcc gaagggcaga
     2941 agtatccggg ttgggttgaa atcagctggt cgcggccgac cggtgccgtg ctggaggcgg
     3001 tcgtcgccaa gctcaaggcg ctgaagctga cgatccgcaa cactccgatg gacggcaacc
     3061 cggtcaccgg aacttgcccc ttcaccggcg aaccggcggt cgagcggatc tacgtggccc
     3121 gcgcatactg acgcatttcg ccaaggtgac ttggcgcgcg atccggcata gcctgcccca
     3181 tcgcgcgatt cgtcgcctag aggggaaaat gtctatgcgt aacgcacttg ctcttgcttt
     3241 cgcctttgcc gccagcgcca cggcggccca ggccgacgaa tggcgtctgg tgtccgagaa
     3301 cgaaggccgg cccggcatcg ccatgtatat cgaccaggcc agcgtggtcc gcaccggcca
     3361 caccgtcacc ggctgggtga tgaccgtgcg cgagtatgat cccaaggccg ctgactggac
     3421 caagtcgctg atcttccggc agatcgattg cgatcgcaac atcgcccaga tgatgcagtc
     3481 caagttctac gggccgaagg gcaacctcct tgaagacacc aaggtcccgg ccaattggca
     3541 gaacatcgtt gacggttcga tgctcgaagg cgtcgccctg gtgatgtgcg gcaaggacgg
     3601 ctacgccagc aacgtcgtgc ccgatccgat ggccgacgcc cgggcccgtt tcgccaagca
     3661 ctgatcataa ggggcttttg cggcttgcct tgtccgcgcc ccgcgtggac aaggcgccat
     3721 gccccgtacc cccaagccgc agcccggcct gccgagccgc cagcagatcc tcgatttcat
     3781 caccagcagc gccgaaccc
//
LOCUS       DASUDV010000568         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1522413,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000568 DASUDV010000000
VERSION     DASUDV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522413"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFV30_12590"
     CDS             <1..240
                     /locus_tag="VFV30_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4848974.1"
                     /translation="ERDYLAVYTNEGRAKGQNRPYPKGTPLNHIGLVVDDLDAAEAAV
                     IAAGLEPFNHGDYEPGRRFYFFDWDGIEFELVSYN"
     gene            complement(237..866)
                     /locus_tag="VFV30_12595"
     CDS             complement(237..866)
                     /locus_tag="VFV30_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX4848975.1"
                     /translation="MCNLYRMTKGTQEVAKLFEVEGDLAANYAHEVYPGFPGLVVAEG
                     RARAMHWGFPLVLTGKQGQKLKPKPVTNAREDKLLTGFWRDSFVHRRCLVPVSQWAEA
                     EGAKGRMTRTWYGLPGEEVFAVAGLWRPSAEWGDVYTLVMVDGHPQMAEVHDRMPLIL
                     SHAAQRYWLEGTADEALALCQTWEDPITVEQTDQRWAGGERPPAQQSLL"
     gene            complement(868..1374)
                     /locus_tag="VFV30_12600"
     CDS             complement(868..1374)
                     /locus_tag="VFV30_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848976.1"
                     /translation="MLAGLVLLAGCDGGAEKAARSEEDKRDAAALVEAINAAPSGPVP
                     TADAASASGKSLSPAAAPTKQPQSSPKRFQALGTEPFWSLEVLDGKLLYTSPEQLTAV
                     AIEAHMTAIGKHGDGGLMWEGRMDGKPVTLTIREGQCSDGMSDTVYPYKSTFTWGDRT
                     EQGCARLK"
     gene            complement(1379..2281)
                     /gene="ubiA"
                     /locus_tag="VFV30_12605"
     CDS             complement(1379..2281)
                     /gene="ubiA"
                     /locus_tag="VFV30_12605"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX4848977.1"
                     /translation="MTAEIVPDTEHRGLVAALPQPWRDYAQLARFDRPIGWWLLFWPC
                     AWGLLLAGGEARWDLLLWFLLGSIAMRGAGCVYNDIVDADIDRQVARTAARPVASGRV
                     SKRAAWVWLVVLCLIGLVVLLQLRWEAQLVALGSLALVAAYPFMKRITPFPQAWLGLV
                     FTWGVPVGWIAVSDTNLPAMAALYAGAVLWCVGYDTIYACQDREDDAMVGIGSSALTL
                     GTRVRAGVAAFYAGAVMLWGSAVWLLRPDWIALLALLPAAAHLGWQVLTLEDNDADNA
                     LARFRSNRTTGLLVALACWVVGNA"
BASE COUNT          393 a          847 c          725 g          340 t
ORIGIN      
        1 gagcgggatt acctggccgt ctacaccaac gagggccgcg ccaaaggaca aaaccgtccc
       61 tatccgaagg gcaccccgct caaccacatc gggctggtcg tcgacgatct ggatgccgcc
      121 gaagccgcgg tgatcgccgc cgggctggag ccgttcaacc acggcgacta cgaacccggc
      181 cggcggttct acttcttcga ctgggacggg atcgagttcg agctggtcag ctacaactag
      241 agcaggctct gctgggctgg cgggcgctcg cccccggccc agcgctggtc ggtctgctcc
      301 accgtgatcg gatcctccca ggtctggcac agcgccaacg cctcgtccgc ggtgccttcc
      361 agccaatagc gctgcgccgc atggctcagg atcagcggca tccggtcgtg cacttcggcc
      421 atctgcgggt gcccgtcgac catcaccagg gtgtagacat cgccccattc ggcgctcggc
      481 cgccatagac ccgcgacggc aaacacctcc tcacccggca ggccgtacca agtgcgcgtc
      541 atccgcccct tcgcgccctc ggcctcggcc cattggctga ccgggacgag gcagcggcgg
      601 tggacgaaac tgtcgcgcca gaagccggtc agcagcttgt cctcgcgggc gttggtgacc
      661 ggcttgggct tgagcttctg gccctgcttg ccggtcagca ccaggggaaa accccaatgc
      721 atcgcccgcg cccgcccctc ggccacgacc agcccaggga aaccgggata gacctcgtgc
      781 gcgtagttgg cggcaagatc gccctccact tcgaacagct tggcgacttc ctgggtgccc
      841 ttggtcatgc ggtagaggtt gcacatgcta cttcaaccgc gcgcagcctt gctcggtccg
      901 atcgccccaa gtgaacgtgg acttgtaggg atagaccgtg tcgctcatcc cgtcgctgca
      961 ctggccttcg cggatcgtca gggtgacggg cttcccgtcc atccggcctt cccacatcag
     1021 gccgccgtca ccgtgcttgc cgatggcggt catatgtgcc tcgatcgcga cggcggttag
     1081 ctgttccgga ctggtgtaga gcagcttgcc atccagcact tcgagcgacc aaaacggctc
     1141 tgtgcccaag gcctgaaaac gctttggcga cgactgtggc tgcttggtcg gcgcggcggc
     1201 cggactgagc gacttgccgg aagcggacgc agcatccgcg gttggcaccg gtccggatgg
     1261 cgcggcattg atcgcctcga caagggctgc cgcatcgcgc ttgtcctctt cggatcgcgc
     1321 cgccttttcc gcgccgccat cacaccccgc cagcagcacc agcccggcca gcaccgcgct
     1381 acgcattgcc caccacccag caggcgagcg cgacgagcag gccggtcgtg cggttggagc
     1441 ggaaccgggc aagggcattg tcggcatcat tgtcctccag cgtcagaacc tgccagccaa
     1501 ggtgcgctgc cgccgggaga agcgcaagca gcgcgatcca gtccggccgc agcagccaca
     1561 cggccgagcc ccacagcatg acggccccgg catagaacgc cgccaccccg gcgcggaccc
     1621 gggtgcccag cgtcagcgcg gaggatccga tcccgaccat cgcgtcatcc tcgcggtcct
     1681 ggcaggcgta gatggtgtca tagcccacgc accacagcac cgccccggca tagagcgcgg
     1741 ccatcgccgg caggttggtg tcgctcactg cgatccaccc caccggcacg ccccaggtga
     1801 agaccagccc cagccaggcc tgcgggaacg gcgtgatccg cttcatgaag ggataggccg
     1861 caaccagcgc caggctgccc agcgcgacca gctgcgcttc ccagcgcagt tgcagcagga
     1921 ccaccaggcc gatcaggcac agcaccacca gccagaccca ggcggcacgc ttgctcaccc
     1981 gcccgctcgc cacggggcgc gccgcggtgc gggcgacctg ccggtcgata tcggcatcga
     2041 cgatgtcgtt gtagacgcag cccgccccgc gcatcgcgat cgagccgagc aggaaccaca
     2101 gcaacaagtc ccaccgcgcc tcgccgcccg ccagcagcag gccccaggcg cagggccaga
     2161 acagcagcca ccagccgatc ggccggtcga accgggccag ttgcgcatag tcgcgccacg
     2221 gctgcggcag ggcggcaacc agcccgcgat gctcggtatc gggaacgatt tcggcggtca
     2281 tgcggcagtt cccgcgccaa ttgtc
//
LOCUS       DASUDV010000569         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1831827,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000569 DASUDV010000000
VERSION     DASUDV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831827"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..2107
                     /locus_tag="VFV30_12610"
     CDS             8..2107
                     /locus_tag="VFV30_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX4848978.1"
                     /translation="MCLAGPALASPYQAKITRDDWGIAHVWGQRDADAVFGMIYAQAE
                     DDFNRIEMNYLTSLGRRAEAEGEGAIWQDLRQRLWIDPEALKADYAKSPQWLKVLMQA
                     WAAGLNQYLADHPEVKPKVITRFEPWMALSFSEGSIGGDIESVSLSQLEAFYSGQPKA
                     LTADERGLVRKEPLGSNGFVIAPAKSASGHAMLLINPHTSFFFRSEQQVASGEGLNAY
                     GAATWGQFFIYQGFNANAGWMHTSSGVDNIDEFAVAVDRSSGRITYRHGDKVREVREK
                     PVTLAYRRADGTMASRTFTTFATHHGPVVREAGGKWIATSLMHRPVAALQQSFLRTKA
                     HDLAGFRKVAALQANSSNNTLFADSKGNVAYLHPQFVPLRDQRFDYRKPVDGNDPRAD
                     WQGLHSLDSLPTVVNPRNGWAMNVNDSPWWAAGPDSPKQGMYPRYVDVVGKEPRTPHA
                     IRVLSARDRFSLDQLLEAAYDPWLPTFSQQLPKLFAAYERAPDADLAEPIALLRGWDC
                     KWGLTSRETSLAIFWAEALWAKGAERASAQGMIVQDWMLTQASDAERLDALREAKARL
                     VADFGSWRVPWGEINRFQRNDGAIRQTFDDAKPSTPVPFASGEWGSLASFGAQRYPGT
                     RRYYGTYGNSFVAVVEFGPRVKARAISAGGESGDPASPHFNDQAARYAAGDLRTVYFY
                     PEDLVVKVRSRKVVQGE"
BASE COUNT          418 a          729 c          745 g          381 t
ORIGIN      
        1 cgtttcgctg tgtctggcgg gtcctgccct ggcatcgccc tatcaggcaa agatcacgcg
       61 tgacgattgg ggcatcgccc atgtctgggg ccagcgcgat gccgatgcgg tgttcgggat
      121 gatctatgcc caggccgagg acgacttcaa ccggatcgag atgaactacc tcaccagcct
      181 gggtcgccgc gccgaggctg aaggcgaagg cgcgatctgg caggacctgc gccagcggct
      241 gtggatcgat cccgaagcgc tcaaggccga ctacgctaaa agcccgcaat ggctgaaggt
      301 gctgatgcag gcctgggcgg cggggctcaa ccagtatctg gccgatcacc ccgaagtgaa
      361 gcccaaggtg atcacccggt tcgaaccatg gatggcgctg tcgttttccg aaggctcgat
      421 cggcggcgat atcgaaagcg tttcgctcag ccagctcgag gcgttctatt cgggccagcc
      481 caaggcgctg accgctgatg agcgcggcct ggtgcgcaag gaaccgcttg gctcgaacgg
      541 cttcgtgatt gcgccggcga aaagcgcatc gggccatgcc atgctgctga tcaacccgca
      601 caccagcttc ttcttccgtt ccgaacagca ggtggcaagc ggcgaggggc tcaatgccta
      661 tggcgcggcc acctggggcc agttcttcat ctaccagggt ttcaatgcga atgccgggtg
      721 gatgcatacc tcgagcgggg tcgacaatat cgacgagttt gccgtggcgg tcgatcgcag
      781 cagcgggcgg atcacctatc gccacggcga caaggtgcgc gaggttcgcg aaaagccggt
      841 cacgctggcc tatcgccgcg ccgatggaac catggccagc cgcaccttca ccacctttgc
      901 cacccaccac ggcccggtgg tgcgcgaggc gggtggcaag tggatcgcaa cgtcgctgat
      961 gcaccgcccg gtggccgcgc tccaacaaag cttcctgcgc accaaggcgc acgatctggc
     1021 cggcttccgc aaggtcgccg cgctgcaggc caacagttcg aacaacaccc tgtttgccga
     1081 cagcaagggc aatgtcgcct atctccaccc gcaattcgtg ccgctgcgcg accagcgctt
     1141 cgactatcgc aagccggtcg acggcaacga tcccagggcc gattggcagg ggctgcacag
     1201 cctcgacagc ttgcccacgg tggtcaaccc ccgcaacggc tgggcaatga acgtcaacga
     1261 ttcgccgtgg tgggcggccg gcccggatag tccgaagcag ggcatgtatc cgcgctatgt
     1321 cgacgtggtc ggcaaggaac cgcgcacccc gcacgcgatc agggtgctgt cggcgcgcga
     1381 tcgcttcagc ctcgatcagc tgctcgaagc ggcctatgat ccctggctgc cgactttttc
     1441 gcagcagctg cccaagctgt tcgccgccta tgagcgggcg cccgatgcgg accttgccga
     1501 accgattgcg ctgctgcgcg gctgggattg caaatggggg ctgacctcgc gcgagaccag
     1561 cctggcgatc ttctgggccg aggcgttgtg ggccaagggc gccgaacgcg ccagcgccca
     1621 ggggatgatc gtccaggact ggatgctgac ccaggccagc gatgccgagc gtctcgacgc
     1681 gctgcgcgaa gccaaggcgc gactggtggc cgatttcggt tcgtggcggg tgccctgggg
     1741 cgagatcaac cgctttcagc gcaacgatgg cgcgatccgc cagaccttcg acgatgccaa
     1801 gccgagcacc ccggtgccct tcgccagcgg cgaatggggc tcactcgcca gtttcggggc
     1861 gcagcgttat ccggggacca ggcgctacta cggcacctat ggcaacagct ttgttgcggt
     1921 ggtcgaattc gggccgcggg tgaaggcgcg ggcgataagc gcgggcggcg aaagcggcga
     1981 tcccgcatcc ccccatttca acgatcaggc cgcgcgctat gccgcgggcg atctgcgcac
     2041 cgtctatttc tatcccgaag acctcgtcgt gaaagtgcgc tcacgtaagg ttgttcaggg
     2101 agaatgacgc gatgaacctg caaaagacat ggctgctgtc cgccatcgcg atgctgtccg
     2161 caacgccgca agaggcgatc gcgagggcaa ccgttcggca agaagccccc gccagcaatc
     2221 tcgaggccaa caaggcgctg gtacgcgctt ttctgaaggc gctggagaaa ggc
//
LOCUS       DASUDV010000570         6292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_108262,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000570 DASUDV010000000
VERSION     DASUDV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108262"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..501)
                     /locus_tag="VFV30_12615"
     CDS             complement(79..501)
                     /locus_tag="VFV30_12615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4848979.1"
                     /translation="MTNRFTKTFTAEPQHIDELGHVNNAVWVDWIQQIATAHWAAVAA
                     PEHQAQYIWVVTRHEIDYRGNVGEGAEVTAETFIPTGPTGARFDRRVDFRGPDGKVIV
                     SSNTTWAMIDRASARLARVRDEVTAPFRPFADEEMRHD"
     gene            complement(498..755)
                     /locus_tag="VFV30_12620"
     CDS             complement(498..755)
                     /locus_tag="VFV30_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848980.1"
                     /translation="MTVKELGWPAIGAMLCALATAAILVSGQFALILGMFPVQLLGLT
                     LLAVAVWQTRRWWLLVFALPMILPLGMWLTLFYQCARGICL"
     gene            complement(752..2806)
                     /locus_tag="VFV30_12625"
     CDS             complement(752..2806)
                     /locus_tag="VFV30_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4848981.1"
                     /translation="MNPATPPRAAKQPHSVTHHGITVTDDYAWLRDPGYPEVKDPAIK
                     DHLKAENAWFEHRMAGQQGRIDALFKEMRARIKEADKSVPQKDGDYLYWIEFEEGAEY
                     KKWWRRPVAGGRDELILDEVALAAGHDYFRLGAIAVSMDGTLLAWSVDDNGSERFTAR
                     FKNLITGEVLPDEIPGTLSSLVWVAGDKGIVYSLANEQWRTDNARLHWLGQPLDSDVE
                     LYHEDDEGFRVGSSLSANEQWLIIGSSDHETSEVRLVPAADPLAAPILVKARQTGVEY
                     DVDERDGTLYIHANDTHENFRLATAPLSDPGTWTTLIEGSDDFYLTGFDLFRDFYAIE
                     GRVRGLDRIEVRYYDDPARIEPIVFPEDSFSAGLGDNPEWAVSALRLSYESMVSPASV
                     LDYDVATRQLTTLKVQEIPSGYDASLYATERLEIAARDGTAIPVSIVYRKDRTGPGPL
                     HLYGYGAYGIAIEPGFSTTRLSLVDRGFAYAIAHIRGGDDLGRAWYKAGKLERRTNTF
                     TDFVDVAKGLIDRGYTAAGRISISGGSAGGELMGAVINSDPGLWGAVVAHVPFVDVLA
                     TMLDPTLPLTPGEWPEWGNPIEDKAAFELIRSYSPYDQVTAQAYPPLLVTAGLNDPRV
                     TYWEPAKWVAKLRELKTDSNELLLKTNMGAGHGGKSGRFESLKETAEEFAFILWQLGV
                     EG"
     gene            2905..3096
                     /locus_tag="VFV30_12630"
     CDS             2905..3096
                     /locus_tag="VFV30_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848982.1"
                     /translation="MRVSLYRSDVNGLFRFEEEHWVWEDIPGYGRNEYWSVRHGSGLY
                     ETFEEAEQDALRTIPWLRA"
     gene            3150..4991
                     /locus_tag="VFV30_12635"
     CDS             3150..4991
                     /locus_tag="VFV30_12635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX4848983.1"
                     /translation="MTDTLAPERPAATAHEQRLAALRAELKARGLDGFVIPISDEHMS
                     EYVGGYAQRLGWLTGFGGSAGTAAVLADKAAIFIDGRYTLQVREQVDGRLYEYQSVPA
                     TSVAAWLAENAPAGARIGFDPWLHGKPWVDATAKALREKGAGLEPVDGNPIDAIWADR
                     PAPSPAPALVHGNQHTGRSSEAKRSEVAEWLTARGLDAAVISALDGVAWLLNIRGTDV
                     ERTPVALSFVLAHADGTADLFIAEEKVTPDLRAHLGNAVTIRPREAFVPQLQALSGKK
                     VAVDPERAVQAVFAALAGAGASVIELTDPTVLPKAQKNPVEQAGQRAAQARDGAAIAR
                     FLHWLSIEAPKGGETELSAAAQLRAFRDASPLLRDLSFDTISAAGPHAASPHYRVDEE
                     SNIPIAPNSIYLVDSGGQYLDGTTDITRTVWVGPGEPTAEHKDRFTRVLKGHIALAQA
                     VFPRGTVGGQLDVLARYALWQAGVDYAHGTGHGVGSFLSVHEGPQRIAKPSGGQAGTG
                     QELLPGMILSNEPGYYKAGEYGIRIENLVLVEPRTIPGQEGEYFGFENLTWAPIDKTL
                     VDTALLTRDELKWWDDYHARVEAILAPQLDGAALDWLKAACAPLTRG"
     gene            5035..5871
                     /locus_tag="VFV30_12640"
     CDS             5035..5871
                     /locus_tag="VFV30_12640"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX4848984.1"
                     /translation="MVDAAFPQWAELALTRVEGFGTDHQLFRLGRDMVVRIPARSEAA
                     SQIHQHAEWLDRFAVLPLAVPRILGLGHPGPDCPYPWCVASWIDGRDAADSGVTEWDH
                     AASALGRFLAELRALDTAGGQASGPANAWRGAPLRKVDSWMRQAIAALDGLADPARLT
                     RLWDEALAVPEWTGAPVWVHGDLHGANILVRGGRVAGVIDFGLVSIGDPACDLAPAWT
                     FLPAAQRETFLQATGLDSAARQRGKGWALYAGAIALAHHRSGNPVLSAMGSRALAAVL
                     DS"
     gene            5918..6286
                     /locus_tag="VFV30_12645"
     CDS             5918..6286
                     /locus_tag="VFV30_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4848985.1"
                     /translation="MIGYVTVGTNDLARAAKFYDAIAAEMGTGRMMEFDTFIAWGTPG
                     GGCGIAATKPYDGAAATVGNGVMVAIEARDNAQVDRLYAIALANGGTDEGAPGPRGDG
                     FYAGYFRDPDGNKLNAFVMG"
BASE COUNT          998 a         2140 c         2122 g         1032 t
ORIGIN      
        1 gcacgctcgg catcccagcc atcgtcttcg gcggcggtgc ggccgccgcg cacccagccg
       61 accgggcgca aggtcacgtc agtcatggcg catctcctca tcggcaaagg ggcggaacgg
      121 cgcggtcacc tcgtcgcgga cccgggcgag gcgcgcgctg gcccggtcga tcattgccca
      181 ggtggtgttg gagctgacga tgaccttgcc atccggcccg cggaagtcga cccgccggtc
      241 aaaccgcgcc ccggtcggcc cggtggggat gaaggtctcg gcggttacct ccgcgccttc
      301 gccgacattg ccgcggtagt cgatctcgtg ccgggtcacg acccagatgt actgggcctg
      361 gtgttccggc gcggcaacgg cggcccagtg cgcggtcgcg atctgctgga tccagtcgac
      421 ccagacggcg ttgttgacgt ggcccagctc gtcgatgtgt tgcggctcgg cggtaaaggt
      481 cttggtgaag cggttggtca caggcaaatt ccccgcgcgc actggtagaa caaggtcaac
      541 cacatcccta atggcaggat catcggcagc gcaaacacaa gcagccacca gcgccgggtc
      601 tgccacaccg cgaccgccaa cagggtcagg ccaagcaact gaacggggaa catcccaagg
      661 atcagcgcga actgccctga aaccagaata gccgcggtgg ccaaggcaca cagcattgcg
      721 ccgattgcag gccagccgag ctcctttacg gtcacccctc aaccccaagc tgccacagga
      781 tgaaggcgaa ctcctccgcc gtctccttca ggctttcgaa ccgcccgctc ttgccgccgt
      841 ggccggcgcc catgttggtc ttgagcagca gctcgttgct gtcggtcttc agctcgcgca
      901 gcttggcgac ccatttggcc ggctcccaat aggtcacccg cggatcgttg agcccggcgg
      961 tgaccagcag cggcggatag gcctgggccg tgacctggtc ataggggctg tagctgcgga
     1021 tcagctcgaa cgcggccttg tcctcgatcg ggttgcccca ttcgggccat tcgcccggcg
     1081 tcagcggcag ggtggggtcg agcatcgtcg ccagcacgtc gacgaacggc acgtgggcca
     1141 ccaccgcgcc ccacaggccg ggatcggaat tgatcaccgc ccccatcagc tcgcccccgg
     1201 ccgaaccgcc ggagatcgaa atccgccccg ccgcggtata gccgcggtcg atcaggccct
     1261 tcgccacgtc gacaaagtcg gtgaaggtgt tggtccgccg ctccagcttg ccggccttgt
     1321 accaggcccg ccccaaatcg tcgccgccgc ggatgtgggc gatggcatag gcaaagcccc
     1381 ggtccaccag gctgagccgg gtggtggaaa agcccggctc gatcgcgatc ccgtaggccc
     1441 catatccata gagatgcagc gggcccgggc ccgtgcggtc cttgcggtag acgatcgaaa
     1501 ccggaatggc cgtgccgtcg cgcgccgcga tctccaaccg ctcggtggca tagagcgagg
     1561 catcgtagcc cgaggggatt tcctggacct tgagcgtggt cagctggcgc gtcgccacat
     1621 cgtaatccag caccgaggcc gggctgacca tgctttcata gctcagccgc agggccgaaa
     1681 ccgcccattc gggattatcc cccagcccgg cggaaaagct gtcttcggga aagacgatcg
     1741 gctcgatccg ggccggatcg tcgtaatagc gcacctcgat ccggtcgagc ccgcggaccc
     1801 gcccttcgat cgcatagaaa tcgcggaaca gatcgaaccc ggtcaggtag aaatcatcgc
     1861 tgccttcgat cagcgtggtc caggtgcccg gatcggacag cggcgcggtg gcgaggcgga
     1921 aattctcgtg cgtgtcgttg gcgtggatgt agagcgtccc gtcccgctca tcgacgtcgt
     1981 attccacccc ggtctggcgg gccttgacca ggatcggcgc ggccagcgga tcggccgccg
     2041 ggaccagccg gacctcgctc gtctcgtgat cggagctgcc gatgatcagc cactgttcgt
     2101 tggccgagag cgaggagccg acgcggaagc cctcgtcgtc ctcgtggtag agctcgacat
     2161 cgctgtccag cggctgaccc agccagtgca gccgggcgtt gtcggtgcgc cactgctcgt
     2221 tggcgagcga atagacgatc cccttgtccc ccgcgaccca gaccaggctg gagagcgtgc
     2281 cggggatttc gtcgggcagc acctcgccgg tgatcaggtt cttgaaccgc gcggtgaagc
     2341 gttccgagcc gttgtcgtcg accgaccagg ccagcagggt gccgtccatc gagaccgcaa
     2401 tcgcgccaag ccggaaatag tcatgcccgg cggccagcgc gacctcgtcg aggatcagtt
     2461 catcccggcc gccagccacg gggcggcgcc accacttctt gtattcggcc ccttcctcga
     2521 actcgatcca gtagaggtag tcgccgtcct tctgcgggac cgacttgtcg gcctccttga
     2581 tccgcgcgcg catttccttg aacagcgcgt cgatccgccc ctgctgcccg gccatgcggt
     2641 gttcgaacca ggcgttctcc gccttcagat ggtccttgat cgcggggtcc ttgacctcgg
     2701 gatagccggg atcgcgcagc caggcgtaat cgtcggtcac ggtgatgccg tggtgggtaa
     2761 cgctgtgggg ctgcttggcg gcacgggggg gagtggcagg gttcatccgg cgggtctatg
     2821 gtgggggctt gaacgccgca agtgctacct cggagattga agcctttgga accgctgaaa
     2881 gtgatcagtt ctgcgtgcgg ttcgatgcgg gtctccctct accgcagcga tgtcaatggg
     2941 ctgttccgct tcgaggagga gcattgggtc tgggaggata tcccgggtta tggccggaac
     3001 gaatactggt ccgtccgcca tggctccggt ctttacgaaa ccttcgagga ggccgagcag
     3061 gacgcgctgc gaacgatccc ctggctgcgg gcctgaggcc tgatcgccgc gccctcgcca
     3121 aagccgcgcg gcgatcctat aacggcgcca tgaccgacac tctcgcgccc gaacgccccg
     3181 ccgccaccgc ccacgaacag cgccttgccg ccttgcgcgc cgagctcaag gcccgcgggc
     3241 ttgatggctt cgtgatcccg atctcggacg agcacatgag cgagtacgtc ggcggctatg
     3301 cccagcggct cggctggctg accggctttg gcggatcggc cggaaccgcg gcggtgctgg
     3361 ccgacaaggc cgcgatcttc attgacgggc gctataccct gcaagtgcgc gaacaggtcg
     3421 atgggcggct gtacgaatac cagtcggtcc cggccacctc ggtcgcggcc tggttggccg
     3481 agaatgcccc ggcgggcgcc cggatcgggt tcgatccgtg gcttcacggc aagccctggg
     3541 tcgacgcgac ggccaaggcc ctgcgcgaga agggggccgg gcttgagccg gtggacggca
     3601 acccgatcga cgcgatctgg gccgaccggc ccgcccccag cccggcgccg gcgctggtcc
     3661 acggcaacca gcacaccggc cggtcgagcg aggccaagcg cagcgaagtg gccgaatggc
     3721 tcaccgcgcg cgggctcgac gcggcggtga tctccgcgct cgacggggtg gcctggctgc
     3781 tcaacatccg cggcaccgat gtggagcgca ccccggtcgc gctgtccttc gtcctggccc
     3841 atgccgatgg cacggccgac ctgttcattg ccgaggaaaa ggtcaccccg gacctgcgcg
     3901 cccacctggg caacgcggtg acgatccgcc cgcgcgaggc ctttgtcccg cagcttcagg
     3961 ccctttccgg caagaaagtg gcggtcgatc ccgaacgcgc ggtccaggcg gtgttcgctg
     4021 ccctggccgg tgccggtgcc agcgtgatcg agctgaccga tccgaccgtc ctgcccaagg
     4081 cgcagaagaa cccggtcgag caggccggcc agcgcgcagc gcaggcccgc gacggcgcgg
     4141 cgatcgcccg gttcctgcac tggctgagca tcgaggcccc caagggcggc gagaccgaac
     4201 tgtccgccgc ggcccagctg cgcgccttcc gcgatgccag cccgctgctg cgcgacctgt
     4261 cgttcgacac gatctccgcc gccgggcccc acgcggccag cccgcattac cgggtggatg
     4321 aggaatcgaa cattccgatc gcccccaatt cgatctacct ggtcgattcg ggcgggcagt
     4381 acctggatgg caccaccgac atcacccgca cggtctgggt cggcccgggc gagcccaccg
     4441 ccgaacacaa ggaccgcttc acccgcgtgc tcaagggcca cattgccctc gcccaggcag
     4501 tgttcccgcg cggtacggtg ggcggccagc tcgacgtgct ggcgcgctat gcgctgtggc
     4561 aggccggcgt cgactatgcc catggcaccg gccacggggt cggcagtttc ctctcggtcc
     4621 acgaagggcc gcagcgcatt gccaagcctt cgggcgggca ggccggtacc gggcaggagc
     4681 tgctccccgg catgatcctc tccaacgagc cgggctatta caaggccggc gaatacggga
     4741 tccggatcga gaacctggtg ctggtcgagc cgcgcacgat cccggggcag gaaggcgaat
     4801 acttcgggtt cgagaacctg acctgggcgc cgatcgacaa gaccctggtc gataccgcgc
     4861 tgctgacccg cgacgagctc aagtggtggg acgattacca cgcccgcgtc gaggcgatcc
     4921 tcgcgcccca gctggacggc gcggcgctgg actggctgaa ggccgcctgc gcgccgctga
     4981 cacggggctg agctgaccag ccgcctggaa ctcccgatca cggccgtccg ccggatggtc
     5041 gacgcagcct tcccgcaatg ggcggagctg gcgctgaccc gggtggaagg ctttggcacc
     5101 gatcatcagc tgttccggct tggccgtgac atggtggtcc gtattcccgc ccggagcgag
     5161 gcagccagcc agatccacca gcacgccgaa tggctggacc gttttgccgt cctgccgctt
     5221 gccgtcccgc gcattctggg cctgggacac cccggaccgg attgccccta tccttggtgc
     5281 gttgcatcgt ggatcgatgg tcgggatgca gcggattctg gcgtgaccga atgggatcat
     5341 gccgcctctg cgctgggccg tttccttgcc gaactgcggg ccctggatac cgccggtgga
     5401 caggcgagcg gccccgccaa tgcctggcgc ggcgctccac tgcgcaaggt cgattcgtgg
     5461 atgcgtcagg ccattgccgc gctggacggc cttgccgacc cggcacggct gacccggctc
     5521 tgggacgagg cgctggccgt tccggagtgg accggcgcgc cggtatgggt ccatggcgat
     5581 ctgcacggcg ccaacattct ggtgcgcggc ggccgggttg cgggcgtgat cgatttcgga
     5641 ctggtttcga ttggcgatcc ggcctgcgat cttgctcccg cctggacgtt tctgcccgcc
     5701 gctcagcgcg agaccttcct gcaggccacc gggttggact cggcggcacg gcagcgcggg
     5761 aagggctggg ccctctatgc cggggccatc gccctggccc atcaccggag cggaaatccg
     5821 gtgctcagcg caatgggcag ccgggcgcta gcagcagtgc ttgactcatg agcaatgttc
     5881 ctatcttgtt ccggctcagc aacaaggagt cgagtcgatg atcggatatg tcaccgtggg
     5941 caccaacgac ctggcccgcg ccgcgaagtt ctatgatgcg atcgcggccg aaatgggcac
     6001 cgggcggatg atggagttcg atactttcat cgcctggggc acgcccggcg gcggctgcgg
     6061 cattgccgcg accaagccct atgacggtgc ggccgcgacg gtcggcaacg gagtgatggt
     6121 ggcgatcgag gcgcgcgata acgcccaggt tgaccggctt tacgcgatcg cgctggccaa
     6181 cggcggcacc gacgaaggcg cgcccggccc gcgcggcgac gggttctatg ccggctactt
     6241 ccgcgatccg gacggcaaca agctcaacgc cttcgtgatg ggttgatgcc gg
//
LOCUS       DASUDV010000571         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_682650,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000571 DASUDV010000000
VERSION     DASUDV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682650"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VFV30_12650"
     CDS             complement(<1..1107)
                     /locus_tag="VFV30_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4848986.1"
                     /translation="MIRKALLGGAAVLAMVAAIPAPVRADASDAQVAAVIDEGLNRSE
                     AMITASQLMDGIGPRLTNSHNYRRAADWATARMTALGLQNVRREGFDFGLGWNVESYR
                     ATMITPRRIDLTAIPVAWSPPTEGVLRAGVVVAPMSKVEHFAAWKGKLAGKVVMISMP
                     GTTSESTKPVFQRFDDKQISEYDRYDLPNFDPAAADRRAKRTNFPLELSRFLKAEGAV
                     AMVRISYRDGMLVHGEGYNYRPGETLALPVFEVAAEDYRRLVRLAAVGPAPEVELQLN
                     AKFDDSTLQAENLFGEIAGSDPKAGYVMAGAHFDSWIAGDGAADNGAGSVVVLEAARI
                     LQRLGVKPRRTIRFALWAGEEQGLLGSRAYIERYL"
     gene            complement(1180..2202)
                     /gene="galE"
                     /locus_tag="VFV30_12655"
     CDS             complement(1180..2202)
                     /gene="galE"
                     /locus_tag="VFV30_12655"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX4848987.1"
                     /translation="MTSLSKPPVLVTGGAGYIGSHAVLALKDAGWPVAVIDNLSTGFR
                     FAIPDGVPLYEGDIADAELLARIFAEQGMGDGQGAIMHFAGSIIVPESVENPLKYYHN
                     NTAKSRALIAAAVAGGVPHFIFSSTAATYGVPRTSPVSEDAEKAPINPYGMSKLMTEI
                     MLGDVSRAHPLNFCALRYFNVAGADPQARTGQSTAGATHLIKVAVEAALGKRAKVDVF
                     GTDFATPDGTGVRDYIHVSDLANAHLLALEALIAEPQRSLTMNCGYGQGYSVLEVLDA
                     VDRVTNQKINRVLGPRRAGDPDSLISDNARIKATLPWQPKYANLDTIVQHALAWERRL
                     GEIRGG"
     gene            2322..>2555
                     /locus_tag="VFV30_12660"
     CDS             2322..>2555
                     /locus_tag="VFV30_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848988.1"
                     /translation="MRPARLFASMTVTAAALALSACATTPYTGPVEVTRFVAQNPDGL
                     GQDPIVVYFPDEVANEVARTAFKTAIEDELRKLG"
BASE COUNT          449 a          845 c          819 g          442 t
ORIGIN      
        1 cagatagcgt tcgatatatg cgcggctgcc gagcaggccc tgctcctcgc ccgcccacag
       61 ggcaaagcgg atcgtccggc gtggtttgac gcccagccgc tgaagaatgc gcgcggcttc
      121 cagcaccacc acgctgccag cgccgttgtc cgccgcgcca tcgcccgcga tccagctgtc
      181 gaagtgcgcg ccggccatga catagccagc cttgggatcg ctgcccgcga tctcgccgaa
      241 caggttttcg gcctgaagcg tgctgtcatc gaacttcgcg ttgagctgca gttcgacctc
      301 cggggcaggg cccacggcgg caagccggac caggcggcga tagtcctcgg ccgccacctc
      361 gaaaaccggc agggccagcg tctcgccggg ccggtaattg tacccctcac catgcaccag
      421 catgccgtcg cggtagctga tccgcaccat cgccaccgcg ccctcggcct tgaggaaccg
      481 gctcagctcc agcgggaaat tggtgcgctt ggcccggcga tcggcggcgg caggatcgaa
      541 attgggcagg tcatagcgat catattcgct gatctgcttg tcatcgaagc gctggaacac
      601 cggcttggtc gattcgctgg tggtgccagg catgctgatc atcacgacct tgccggcgag
      661 cttgcccttc caggcggcaa agtgttcaac cttgctcatc ggcgcgacca ccacgccggc
      721 gcgcagcacg ccttcggtcg gtggtgacca ggcgacgggg atggcggtga ggtcgatccg
      781 gcgtggggtg atcatcgtcg cgcgatagct ttcgacgttc cagcccaggc cgaagtcgaa
      841 gccctcgcgg cgcacgtttt gcaggcccag ggccgtcatc cgcgccgtcg cccagtcagc
      901 cgcgcggcgg tagttgtgcg aattggtcag gcgcggaccg atcccgtcca tcagctgcga
      961 ggcggtgatc attgcctcgc tgcgattgag gccttcgtcg atcacggcgg caacctgcgc
     1021 atcgctggca tcggcccgca ccggtgccgg gattgcagcc accatggcaa gcactgccgc
     1081 accgccgagc aaggctttcc ggatcatcaa tcatctcccc catctggtca gaccggcttg
     1141 ctggcacagc caaccggtca ggccaagggg gccgaaggac tatccgccgc ggatttcgcc
     1201 caggcgccgc tcccaggcca gggcgtgctg cacgatggtg tccaggttgg cgtatttcgg
     1261 ctgccagggc agcgtcgcct tgatccgcgc attgtccgag atgagcgaat ccggatcgcc
     1321 ggcgcggcgc gggccgagca cgcggttgat cttctggttg gtcacgcggt cgaccgcgtc
     1381 gagcacttcg agcaccgaat agccctggcc atagccgcag ttcatcgtca gcgagcgctg
     1441 cggctctgcg atcagcgctt caagcgcaag gaggtgggca ttggccaggt ccgagacatg
     1501 gatgtaatcg cgcaccccgg tgccgtcggg cgtggcaaaa tcggtcccga acacgtcaac
     1561 cttcgcgcgc ttgcccagcg ccgcttcgac cgcgaccttg atcagatggg tggcgccagc
     1621 ggtcgattgg ccggtgcggg cctgcggatc ggcgccggca acgttgaaat agcgcagggc
     1681 acagaaattg agcggatgag cccgcgagac gtcgcccagc atgatctcgg tcatcagctt
     1741 ggacatgcca tagggattga tcggcgcctt ctcggcatct tcgctgaccg gcgaggtcct
     1801 cggcacgcca taggtcgcgg cggtcgagct gaagatgaag tgcggcaccc cgcccgccac
     1861 cgcggcggca atcagcgccc ggctcttggc ggtgttgttg tgatagtact tgagcgggtt
     1921 ctcgaccgat tcgggcacga tgatcgaacc ggcgaaatgc atgatcgcac cttggccgtc
     1981 gcccatgccc tgttcggcaa agatccgcgc cagcagctcg gcatcggcaa tatcgccttc
     2041 gtagagcggg acgccgtcag ggatagcgaa acggaagccg gtcgacaggt tgtcgatcac
     2101 ggcgacaggc cagccagcat ccttgagagc cagcacggca tggctgccga tatagccggc
     2161 accgccagtc acaagcacgg gaggtttgct gagcgaagtc atcgaacggc ccttagcaca
     2221 ccgattgctt agaaaacgtc aaccttgtgc aaggcaaagc ggagtctgtg cacagccgcc
     2281 ctcccgccat ataagcggtt ggccacgaaa ggaacactgc catgcgtccc gcccgcctgt
     2341 ttgcctcgat gactgtaact gccgctgcat tggcgctatc cgcctgtgcc accacgccct
     2401 acaccggccc ggtcgaagtg acccggttcg tcgcgcagaa ccccgatggg ctcgggcaag
     2461 acccgatcgt ggtctatttc ccggatgagg ttgccaacga ggtcgcacgc accgcgttca
     2521 agaccgccat cgaggacgag ctgcgcaagc tcggc
//
LOCUS       DASUDV010000572         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_771427,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000572 DASUDV010000000
VERSION     DASUDV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771427"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFV30_12665"
     CDS             <1..294
                     /locus_tag="VFV30_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX4848989.1"
                     /translation="GNRLAVSHGTLTGGLVGDIVDSSVKEQVLRNCVAGADGAHSVAT
                     GDGANDIPMLVAAHYGVAYHAKPKARAAANGAIDRGDLTDVLRLLGIPESDWA"
     gene            complement(291..872)
                     /locus_tag="VFV30_12670"
     CDS             complement(291..872)
                     /locus_tag="VFV30_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848990.1"
                     /translation="MTMMRRWPMLLGLVAGLGAVWLGLTTGSDAADQWSRATRWTARI
                     GLPIFLLTYMASSLGRLWPGDLTRRLWQGRRWWGLGFAACHTVHLYALIRALDASGEV
                     RTLASLVPGGTAYLLIFLMALTSSDAAMRALGRNWKRLHTFGIHYVWLIFTLAYAGRL
                     MEAETRPEAVYGVTLCLAALAVRIAAWRKGRRA"
     gene            985..1821
                     /locus_tag="VFV30_12675"
     CDS             985..1821
                     /locus_tag="VFV30_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEX4848991.1"
                     /translation="MNMMTAVAAGPQAHVDFASPDLPMMLPGRPKMRYDLPRAWRHFK
                     ELVKDKEDTSQVTEIFQALPWRGVYDAALAFLKTDRAQEIRRKEPSLVKILDDHETLR
                     KLPAGSLAHAYCDFMEREGLSAQGLVDELDKNRPADMYWDDQVSWYFNRMRDTHDLLH
                     VLTGFGRDALGEQCVLAFTYSQQPSPAHLFLGYAGGLEIRKHPVKVPVFRAVREAQKM
                     GKACPRLVETSITELLAMPLEQARATLNIAAPRYYQQAHAIWRSKGVDPYDLMAPVQQ
                     AA"
     gene            complement(1858..3252)
                     /locus_tag="VFV30_12680"
     CDS             complement(1858..3252)
                     /locus_tag="VFV30_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848992.1"
                     /translation="MTADAAIDPVPPSALPRFTPVSTPAPAMPAAWSATVLLHPFSPP
                     PSQYPHIDLPFFQLCIAQLDCLAGEYFSIRVAGTEYGQWWYMMDKDGTRVSTNGGIFW
                     KDVDMGWSLPTDWYGAQAATARCPGAAPLNWMSDRMAEWWTIAVPIATKEPQRLGLHA
                     EAATWLWFDAETKAPMRMMFGDGPPMYHYGDPTQLAFLQMFSFSYFTNYSAFTAEAAP
                     PKPDRWIVPSIAGFSVGNPGGYKPFVWNGNFGMTAFMTPVNGYFNPLPTRVLYKWTPD
                     AAYSQYSDRAQDTLMFYNYNPNQPGGLRPLTQQTALLTGPAPSGMPAPPHSASGFLYS
                     EYTTGGPDCQSGASFPFGQEPPNWVSVPAVEGTVWATIVDNPELCPGQTVLIYSVLFP
                     PSAPRYPEATYLWTWYAPQAGGDGTNSRPVTFMQSQSTLSEGTSLALADYYYYQPFST
                     PIDPANFAIPPCCL"
     gene            complement(3312..>3737)
                     /locus_tag="VFV30_12685"
     CDS             complement(3312..>3737)
                     /locus_tag="VFV30_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4848993.1"
                     /translation="AALFTALGGKAEEADAALAQTSGQTPGPSIWQKLLGLVEHELGP
                     DVLAWLALDLVRTDWAAIMAAGQVPLDQGYDAFMTAIQADFQKARDIVVHNPNGIQFN
                     AAPPVLHMIITGMYACAPQVTVRTIATVMNYTEMESQPA"
BASE COUNT          642 a         1232 c         1265 g          598 t
ORIGIN      
        1 ggtaaccggc tggcagtcag ccacggcacg ctgaccgggg gactggttgg cgatatcgtc
       61 gacagttcgg taaaagagca ggtcctgcgc aactgcgtgg ccggggccga cggagcgcac
      121 agcgtggcaa cgggcgacgg ggccaacgac atcccgatgc tggtggcggc gcattacggc
      181 gtggcctatc atgccaagcc caaggcccgc gccgccgcca acggcgcgat cgaccggggc
      241 gacctgaccg acgtcctgcg gctgctgggc attcccgaaa gcgactgggc ctaggcccgt
      301 cgccccttgc gccaggccgc gatccgcact gccagcgcgg ccaggcacag cgtcacgcca
      361 tagacggcct cgggccgcgt ttcggcctcc atcagccgcc cggcataggc cagggtgaag
      421 atcagccaga catagtggat gccgaaggtg tgcagccgct tccagttgcg ccccagcgcc
      481 cgcatcgccg cgtcgctgga ggtcagcgcc atcaggaaga tcagcagata ggccgtgccg
      541 ccgggcacca gcgaagcgag ggtgcgcact tcaccgctgg catccagtgc gcggatcagc
      601 gcgtaaaggt gcaccgtgtg gcaggcggca aagcccaggc cccaccagcg gcggccctgc
      661 cacagccgcc gcgtgagatc ccccggccag agccgtccga gcgaggaggc catataggtc
      721 agcaggaaga tcggcagccc gatccgcgcg gtccagcgcg tggcgcggct ccactggtcg
      781 gcggcatcgc tgccggtggt caggcccagc cagaccgcgc caagcccggc cacaagcccc
      841 agcagcatcg gccagcgccg catcatcgtc atgtccccgt cctccccttg gcaacggggg
      901 cgcactctgc cgcgcggatg ccgattgtcc agtgcagttg gtctattgac atttctgtaa
      961 gtagttcgca ttaacaggct tgccatgaac atgatgaccg ctgttgccgc cgggcctcag
     1021 gcccatgtcg atttcgcttc gccggatctg cccatgatgc tgccgggccg gccgaagatg
     1081 cggtatgacc tgccgcgcgc ctggcgccac ttcaaggaac tggtgaagga caaggaagac
     1141 acctcgcagg tgaccgagat ctttcaggcg ctgccgtggc gcggggtcta tgatgccgcg
     1201 ctggccttcc tgaagaccga ccgcgcgcag gaaatccgcc gcaaggaacc ttcgctggtc
     1261 aagatcctcg acgatcacga aaccctgcgc aagctgcccg caggcagcct ggcccacgcc
     1321 tattgtgact tcatggagcg cgaagggctt tcggcccagg ggctggttga cgagctcgac
     1381 aagaaccgcc cggccgacat gtactgggac gatcaggtca gctggtactt caaccgcatg
     1441 cgcgacaccc acgatctgct ccacgtgctg accggtttcg gccgcgatgc gctgggcgaa
     1501 cagtgcgtgc tggcctttac ctacagccag cagccgagcc cggcgcacct gttcctgggc
     1561 tatgccggcg gcctggaaat ccgcaagcac ccggtcaagg tgccggtgtt ccgcgcggtc
     1621 cgcgaagccc agaagatggg caaggcctgc ccgcggctgg tcgaaacctc gatcaccgaa
     1681 ctgctggcca tgccgctgga acaggcgcgg gccacgctca acatcgccgc gccgcgctat
     1741 taccagcagg cccacgccat ctggcgctcc aaaggcgtcg atccctacga tctgatggcg
     1801 ccggtccagc aggcagcctg agccggcggg ggctttcccc caccggcccg gcaaagccta
     1861 gaggcagcag ggcggaatcg cgaaattcgc cggatcgatc ggggtcgaga acggctggta
     1921 gtaatagtag tccgccaggg ccaggctggt cccttcgctg agcgttgatt gcgactgcat
     1981 gaaggtgacc ggccggctgt tcgttccgtc cccgccggct tgcggcgcgt accaggtcca
     2041 cagataggtc gcttcgggat agcggggcgc agacggcgga aacagcaccg aatagatcag
     2101 caccgtctgg cccgggcaca gctccggatt gtcaacgatc gtggcccaga ccgttccctc
     2161 caccgccggg accgaaaccc agttgggcgg ctcctgcccg aacgggaagc tggcccccga
     2221 ttggcagtca ggcccgccgg tggtgtattc cgagtagaga aagcccgatg ccgaatgggg
     2281 cggtgccggc atgccgctgg gcgccgggcc ggtcagcagg gccgtctgtt gggtcagcgg
     2341 ccgcagcccg cccggctggt tcgggttgta gttgtagaac atcagcgtgt cctgcgcccg
     2401 gtcgctgtac tgcgaatagg cggcgtccgg cgtccacttg tacagcaccc gggtcggcag
     2461 cgggttgaag tagccgttca ccggggtcat gaacgcggtc atcccgaaat tcccgttcca
     2521 cacgaacggc ttgtacccac cgggattgcc cacgctgaag ccggctatcg aggggacaat
     2581 ccagcgatcg ggcttgggcg gcgcggcctc ggcggtgaaa gcgctgtagt tggtgaaata
     2641 gctgaacgag aacatctgca ggaacgccag ctgggtcgga tcgccatagt ggtacatcgg
     2701 cgggccatcg ccgaacatca tccgcatcgg ggccttggtt tcggcgtcga accatagcca
     2761 ggtcgccgct tcggcatgga ggcccaagcg ctgcggctcc ttggtcgcga tcgggaccgc
     2821 gatggtccac cattccgcca tccggtcgct catccagttg agcggcgcgg cgccggggca
     2881 gcgggcggtg gcggcctggg cgccgtacca gtcggtcggc agcgaccagc ccatgtcgac
     2941 atccttccag aagatcccgc cgttggtcga aacccgggtg ccgtccttgt ccatcatgta
     3001 ccaccactgg ccgtattcgg taccggccac gcggatggag aaatactcac cggccaggca
     3061 atccagctgg gcaatgcaca gctggaagaa cggcagatcg atgtggggat actggctggg
     3121 cggcgggctg aaggggtgga gcagcacggt cgcgctccac gccgccggca tggcgggcgc
     3181 gggcgtggaa accggggtga agcgcggcag cgccgaaggc ggcaccggat cgattgcagc
     3241 gtctgcagtc atggttgtct ctccactgtt gttctggttt tcgcgaagac ccggattgtc
     3301 cgggatgggg atcaggccgg ttgggactcc atctcggtgt agttcatcac cgtcgcgatc
     3361 gtccgcacgg tcacctgcgg agcgcaggcg tacatgccgg taatgatcat gtgcagcacc
     3421 ggcggcgcgg cgttgaactg gatcccgttc gggttgtgga ccacgatgtc gcgggccttc
     3481 tggaaatcgg cctggatcgc ggtcatgaag gcgtcatagc cctgatcgag cggcacttgc
     3541 cccgcggcca tgatcgccgc ccagtcggtc cgcaccaggt cgagcgccag ccaggccagc
     3601 acgtccggtc ccagctcgtg ctcgaccagg ccgagcagct tttgccagat gctggggccc
     3661 ggggtctgcc cgctggtctg ggcgagggcc gcatcggcct cctcggcctt gccgcccagc
     3721 gcggtgaaca gcgcggc
//
LOCUS       DASUDV010000573         9250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2141066,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000573 DASUDV010000000
VERSION     DASUDV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141066"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="mnmG"
                     /locus_tag="VFV30_12690"
     CDS             complement(<1..1352)
                     /gene="mnmG"
                     /locus_tag="VFV30_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX4848994.1"
                     /translation="MQQFDVLVVGGGHAGVEAAAVAARMGAHTALVSFDPEAIGAMSC
                     NPAIGGLGKGHLVREVDAFDGIIARAADAGAIHYRMLNRSKGSAVQGPRVQADRKRFK
                     AEVQRLLARQGDLTVIAGEAAALRMDGGRVSGLELADGTALAAPAVILCTGTFLGGIL
                     FRGEERMVGGRIGEASAQRLAAQLREARLPLARLKTGTPPRLDGRTIDWARLEEQPSE
                     VEPWTMSPLGAGRVLPQVFCAITRTNARSHDVIRANLHRSPLFSGAIGANGPRYCPSI
                     EDKIHRFGDRDGHQVFLEPEGLDTHLVYPNGISTSLPADVQLAMLRTMEGLEGVEMVV
                     PGYAVEYDHIDPRALRPSLELRDLPGLYCAGQINGTTGYEEAAAQGLVAGMHAAAAVL
                     GRDGPAIDRANSYMAVMIDDLTLHGVTEPYRMLTARAEYRLRLRANNAASRLTPLGLA
                     L"
     gene            complement(1448..2725)
                     /gene="mnmE"
                     /locus_tag="VFV30_12695"
     CDS             complement(1448..2725)
                     /gene="mnmE"
                     /locus_tag="VFV30_12695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX4848995.1"
                     /translation="MTDTIFALSSGAPPAAIGVVRVSGPRARDALLDLAGRVPAPRRA
                     ALARLRDRQGAVIDEALVLWLPGPGTETGEDTVELQCHGGRAVIAAVETALAAQPGLR
                     RAQPGEFTRRAFLNGKIDLAEAEGLADLLEAETELQRLSAMAMAGGAFSREVEGWRAE
                     LLGLSAAVEAVLDFADEDDVATLPADFALRIEALGKAIKAWLDRPRAELLKDGFRVVV
                     AGPPNAGKSTLFNVLVEAEAAIATPIAGTTRDVLTRPVAIAGVPFLFADTAGLHDAVD
                     DVVEAIGIERALEALDRADLVLWLGPEGMGPPGAWEIEAQTDRDGHSAKTNPRHRISA
                     RTGAGIESLRSDLVETASRALPKPGEGALNERQHELLGAARSALAQAAEHHDPLLVAE
                     ALRLARVAFDGLLGRTATEDMLDALFGRFCIGK"
     gene            complement(2763..3020)
                     /locus_tag="VFV30_12700"
     CDS             complement(2763..3020)
                     /locus_tag="VFV30_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HEX4848996.1"
                     /translation="MKVHVEIDCTPEEARAFLGLPDVSKANSVYVDVISKAMKGAGSV
                     EQVQDFAKNIAPMGQVGLKLFQQFMESSNAFAAGAGKKKDG"
     gene            3133..3831
                     /locus_tag="VFV30_12705"
     CDS             3133..3831
                     /locus_tag="VFV30_12705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX4848997.1"
                     /translation="MTETVHIPSIKDDGAIPAYVARPQGPAKAAIIVIPEIFGVNPGI
                     RQKADSWAAKGYLAVAPDIFWRFAPGVELNPDVEAELNEAFGYFQQYDPTDGVYDIES
                     TIKWIRAEAGAQKVGCSGYCLGGRLAYMAAARTDIDASVGYYGVMIDTMLDEAHRIAN
                     PLLLHIAGEDQFVSAEAQAAMHAGLDGHPKVTLYDYPGMPHGFAAEMGDRRQDAEAKL
                     ADSRTEAFLAEQLG"
     gene            complement(3843..4973)
                     /locus_tag="VFV30_12710"
     CDS             complement(3843..4973)
                     /locus_tag="VFV30_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX4848998.1"
                     /translation="MTSDPRAAIDQGLAALANRDAARAAALLREAAAAVPAAEMPWTA
                     LGNAELALGNRDAAEAALERQLEQTPRDIGALLLKAWLREQAGDARSASSFYRAALNQ
                     AAVDGRVPPALARLHQHAGTYVQEASGGFEAYLLERIGTDLSPTMTEALELLTGKREL
                     YLQEPSVFYYPGLPQKRFYDPADFPWLEPMLALVPAMQAELAAVLGAGAEGFAPYVHR
                     KRHRPAPNSPLLDSEAWTAFHFWRDGERDEDNAARCPATMEALGHAPMPQIAGRSPNA
                     HWSRLLPGAHIAAHTGMLNTRLICHIPIKTAPDCWFRVGSEVRGWVDGVPLVFDDSIN
                     HEAKNDGPEERVVLLFEIWRPEIDPADREAIGRIFQAIGEYD"
     gene            5054..5452
                     /locus_tag="VFV30_12715"
     CDS             5054..5452
                     /locus_tag="VFV30_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4848999.1"
                     /translation="MNARTGVAHWHAYMESGGDAALLDALTADDAVFHSPVVHSPQQG
                     KAKVMLYLLSAAKVLGNESFTYVREVIEGNDALLEFTSEIDGIHINGIDLITFDDDGK
                     IKDFKVMVRPVKAVNKLWEMMAAQLQAASG"
     gene            complement(5434..5892)
                     /locus_tag="VFV30_12720"
     CDS             complement(5434..5892)
                     /locus_tag="VFV30_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849000.1"
                     /translation="MDDELPEDAMVTVAALVSRVEALVVASMLEAGGILVHVGGAAHA
                     SAEVNTAALGGHRLWVPASQHMAASELLLEVLGEEEWAFSRGLQRAVLRFAGAWAGVS
                     TAAALVICSLGKAPMLWALMAPFSALTIPVNPQGRGDYYLHADARYPEAA"
     gene            complement(5905..6456)
                     /locus_tag="VFV30_12725"
     CDS             complement(5905..6456)
                     /locus_tag="VFV30_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4849001.1"
                     /translation="MGLLVPRHAVLTGAPGAGKTTLLDAAAAAGIQTSPEVARQLLKA
                     PGGMALRENDPLGFAEAMLEAHRREFERHGDHPEPVLFDRGFPDVVGFLDVSGLAVPP
                     AVDHACRTLRYAGPILRAPAWAAIYAQDAERIQDWEQAVASDEAVTAAWKRYGYEVTD
                     LPLEGVDKRLEFLRKMVDRSPSA"
     gene            complement(6458..7714)
                     /gene="rho"
                     /locus_tag="VFV30_12730"
     CDS             complement(6458..7714)
                     /gene="rho"
                     /locus_tag="VFV30_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX4849002.1"
                     /translation="MHLKELKKKTSAELVEMAEELGVEGASTLRRQDLMFAILKEVAE
                     DGEEIIGQGTIEVLADGFGFLRSPEANYLAGPDDIYVSPNQVRKWGLRTGDTVEGEIR
                     APKDGERYFAITKLVSVNYDDPDVVRHRVNFDNLTPLYPDSKLSLDTLDPTVKDKSAR
                     VIDIISPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVFLLVLLVDERPEEVTD
                     MQRSVKGEVISSTFDEPAQRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGT
                     VDAMEFLLDKMKDSKTNEDFFATMNQ"
     gene            complement(7855..8298)
                     /locus_tag="VFV30_12735"
     CDS             complement(7855..8298)
                     /locus_tag="VFV30_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX4849003.1"
                     /translation="MNWMLSTTYLWLKAGHIIFVIFWMAGLFMLPRFFVYHQEAEPDS
                     PENALWIEREARLLKIILWPSLVVAWVLGLALAYATGAFGQGWFHVKLAAVLALTSYH
                     IWLAGYHAALTRGERRLTGRKLRMINEVPGIAAALIVIMVVLRPF"
     gene            complement(8314..>9250)
                     /gene="hemE"
                     /locus_tag="VFV30_12740"
     CDS             complement(8314..>9250)
                     /gene="hemE"
                     /locus_tag="VFV30_12740"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX4849004.1"
                     /translation="EYRALRAEKGGFLALVYDSEAAAEITIQPLRRYGMDGAILFSDI
                     LIVPYAMGQDLEFLVGEGPHLSPRLADAALASLHAVPERLTPIYDTVKLVKAQLPPGK
                     TLLGFAGSPWTVATYMVAGEGSRDQHATREMAYRDPSAFQAIIDAIVAVTIEYLAGQI
                     AAGAEAVQLFDSWAGSLAPSEFERWVIAPNAAIVKALRARFPHVPIIGFPKGAGEKLP
                     AYARETRVDALGLDETIDPLWAAKALPAGLPVQGNLDPLLLLSGGAEMERQTLRILDA
                     FADRPHVFNLGHGIGQTTPVEHVGHLLALVRGWQG"
BASE COUNT         1516 a         3186 c         3048 g         1500 t
ORIGIN      
        1 agggccaggc ccagcggggt gagccgcgag gcggcgttgt tggcgcggag ccgcagccgg
       61 tactcggccc gggcggtcag catccggtag ggctcggtca cgccgtgcag ggtcagatcg
      121 tcgatcatca ccgccatgta gctgttcgcc cggtcgatcg ccggaccgtc acgccccagc
      181 acggccgcgg cggcgtgcat gcccgcgacc agaccctgcg ccgcggcctc ttcatagccg
      241 gtggtgccgt tgatctgccc ggcgcaatag agacccggca ggtcccgcag ctccagactg
      301 gggcgcagcg cgcgcggatc gatgtggtcg tattccacgg catagcccgg cacgaccatc
      361 tccacacctt ccagcccctc catcgtccgc agcatggcga gctggacgtc ggccggcagc
      421 gaagtgctga tcccgttggg atagaccagg tgggtgtcga gcccttccgg ctccaggaag
      481 acctgatggc cgtcgcggtc gccaaagcgg tggatcttgt cctcgatcga ggggcaatag
      541 cgcggcccgt tggccccgat cgcgccggag aacaacggcg agcggtgcag gttggcgcgg
      601 atcacatcat ggctgcgcgc gttggtgcgg gtgatcgcgc agaacacctg cggcaggacc
      661 cgccctgccc ccagcggcga catggtccat ggttcaacct cgcttggctg ctcctccagc
      721 cgcgcccagt cgatcgtgcg cccgtcgagc cgcggcgggg ttccggtctt gagccgggcc
      781 aagggcaggc gcgcttcgcg cagctgcgcg gccagccgct gggccgaagc ctcgccaatc
      841 cgcccgccga ccatccgctc ttcgccgcga aacaggatcc cgccaaggaa ggtgcccgtg
      901 cacaggatca ccgccggcgc ggccagcgcg gtcccgtccg ccagctccag cccgctgacc
      961 cggccgccat ccatgcgcag ggccgcagcc tcgccggcga tcacggtcag atcgccctgg
     1021 cgtgccagca agcgctggac ctccgccttg aaccgcttgc gatcagcctg gacgcgcggt
     1081 ccctgcacgg ccgagccctt ggagcggttg agcatgcggt aatggatcgc cccggcatcg
     1141 gcagcgcggg cgatgatgcc gtcaaaggca tcgacctcgc ggaccaggtg gcccttgccc
     1201 agtccaccga tcgcggggtt gcagctcatt gccccgatcg cctcgggatc gaagctgacc
     1261 agcgcggtgt gcgcgcccat ccgcgccgcc accgccgcgg cttccacccc ggcatggccc
     1321 ccaccgacca caaggacatc gaactgctgc atgattgcct gcgcctttag agcatcgcgg
     1381 cgcaaagtgg gaaccggttt tcgtctgtcc ggcggcgatt gcggccagag tgtttcacgt
     1441 ggaacagtta tttgccgatg cagaaccgtc cgaacagggc gtcgagcatg tcctcggtcg
     1501 cggtgcggcc gagcagaccg tcaaaggcga cgcgggccag ccgcagcgct tcggccacca
     1561 gcaacggatc gtggtgctcg gccgcctggg ccagcgcgct gcgcgccgcg ccgagcaatt
     1621 cgtgctgccg ctcgttgagc gccccctcgc ccggcttggg cagagcccgg ctggcggtct
     1681 ccaccaggtc gctgcgcagc gattcgatcc ccgcaccggt ccgggccgaa atgcggtggc
     1741 gcgggttggt cttggcggaa tgcccgtccc ggtcggtctg ggcttcgatc tcccaggcgc
     1801 ccggcggccc catcccttcc ggccccaacc acaggaccag atcggcccgg tccagcgctt
     1861 ccagcgcgcg ttcgatcccg atcgcctcga ccacgtcgtc caccgcatcg tgcagaccgg
     1921 cggtgtcggc aaacaggaac ggcaccccgg cgattgccac cgggcgagtc aggacatcgc
     1981 gggtggtccc ggcaatcggc gtggcaatgg ccgcctccgc ctcaaccaag acgttgaaaa
     2041 gagtgctttt cccggcgttc ggcgggcctg cgacgaccac ccggaagccg tccttgagca
     2101 attcggcccg cggccggtca agccaggcct tgatcgcctt gccgagggcc tcgatccgca
     2161 gcgcgaagtc ggccggaagg gtggccacgt cgtcctcgtc ggcaaaatcg agcacggcct
     2221 cgaccgcggc tgacagcccg agcagctcgg cccgccagcc ctccacctcg cgcgagaagg
     2281 ccccgcccgc catggccatg gccgagaggc gctgcagctc ggtttccgcc tcgagcaggt
     2341 cggccagccc ttcggcttcg gcgaggtcga tcttgccgtt gaggaaggcg cggcgggtaa
     2401 attcgcccgg ctgggcccgg cgcagcccgg gctgggcggc cagcgcggtt tccaccgcgg
     2461 cgatcaccgc ccggcccccg tggcattgca gctcaaccgt gtcttcgccg gtttcggtcc
     2521 ccggccccgg cagccacagc accagggcct cgtcgataac ggccccctgg cggtcgcgca
     2581 gccgggccag ggcggcgcgg cgcggcgcgg ggacccgccc ggccaggtcc agcagcgcat
     2641 cgcgcgcgcg cggcccgctg acccgcacga ctccgatcgc cgccgggggc gcgccgctgg
     2701 acaaggcaaa gatcgtatcc gtcataacag gggcttggcg caccctttcc gcagggaaag
     2761 gttcaaccgt ccttcttctt cccggcccct gcggcaaagg cgttgctgct ttccatgaac
     2821 tgctggaaca gcttcaagcc gacctggccc atcggggcga tgttcttggc gaagtcttgc
     2881 acctgctcaa ccgagccggc gcccttcatc gccttggaga tgacatcgac atagaccgaa
     2941 ttggccttgc tgacatcggg caagcccaga aaggcccgtg cctcttcggg ggtgcaatcg
     3001 atctcgacat ggactttcat tgcaagctcc ttggcgacgc ccagttctcc cgaaggggaa
     3061 gctggcatag ccccttcaca aagtccacgg ctggcttagg aagaccgcaa agctttaccg
     3121 gagaacccag ccatgaccga aaccgtgcac attccctcga tcaaggacga cggcgcgatt
     3181 cccgcctatg ttgcccgccc gcaagggccg gccaaggccg cgatcatcgt cattccggaa
     3241 atcttcggcg tgaaccccgg gatccgccag aaggccgaca gctgggccgc caagggctat
     3301 ctcgcggtcg cgcccgacat cttctggcgc tttgcccccg gggtggaact caaccccgat
     3361 gtcgaggcgg agctcaacga ggctttcggc tatttccagc agtacgatcc caccgacggg
     3421 gtctatgata tcgaatcgac catcaagtgg atccgcgccg aggccggcgc ccagaaggtc
     3481 ggctgctcgg gttactgcct cggcggccgg ctcgcctaca tggccgccgc gcgcaccgat
     3541 atcgatgcct cggtcgggta ttacggcgtg atgatcgaca ccatgctgga cgaggcgcac
     3601 cgcatcgcca acccgctgct gctgcacatt gccggcgagg atcagttcgt ctcggccgag
     3661 gcccaggcag cgatgcacgc cgggctggac ggccatccca aggtcaccct ctacgactat
     3721 cccggcatgc cgcacggctt tgccgcggaa atgggtgacc gccgccagga cgccgaagcc
     3781 aagctggccg acagccgcac cgaagcattc ctggccgagc aactcggcta ggccgggcgc
     3841 ggctaatcgt attcgccaat ggcctggaat atccggccga tcgcctcgcg gtcggccggg
     3901 tcgatctcgg gccgccagat ctcgaacaac aggacgaccc gttcctccgg accgtcgttc
     3961 ttggcctcgt ggttgatcga atcgtcgaac accagcggca cgccgtccac ccagccgcgc
     4021 acttcgctgc cgacccggaa ccagcaatca ggcgcggtct tgatcgggat gtggcagatc
     4081 agccgggtgt tgagcatgcc ggtgtgggcc gcgatatgcg cccccggcaa gagccgcgac
     4141 cagtgcgcgt tgggcgagcg gccggcgatc tgcggcatcg gtgcatggcc cagcgcctcc
     4201 atcgtcgccg ggcagcgcgc ggcattgtcc tcgtcccgtt cgccgtcgcg ccagaaatgg
     4261 aacgcggtcc aggcctcgct gtcgagcagc ggtgaattgg gcgccggacg gtggcgcttg
     4321 cggtgcacgt aaggcgcaaa gccttccgcc cctgccccca gcaccgcggc cagctcggcc
     4381 tgcatcgccg ggaccagggc cagcatcggc tccagccagg ggaaatccgc cggatcgtag
     4441 aagcgtttct gcggcagccc cggatagtag aacaccgaag gctcctgcag atagagctcg
     4501 cgcttgccgg tcagcagctc cagcgcctcg gtcatggtcg gggacaggtc ggtgccgatc
     4561 cgctcgagca gataggcctc aaacccgccg ctggcctcct gaacataggt tccggcgtgc
     4621 tggtgcagcc gggccagcgc cggcggcacc cggccgtcga ccgccgcctg gttgagcgcg
     4681 gcgcggtaga agctggaggc cgaacgggca tcgccggcct gttcgcgcag ccaggctttc
     4741 agcagcaagg cgccgatatc gcgcggggtc tgctccagct gccgttccag cgccgcttcg
     4801 gccgcgtcgc ggttgcccag ggccagctcg gcattgccca gcgcagtcca cggcatctcg
     4861 gcggcgggaa ccgcggcggc ggcttcgcgc agcaaggccg cggcgcgcgc cgcatcgcgg
     4921 ttcgccaatg cggcaaggcc ctggtcgatg gcggcgcggg gatcgcttgt catgtacggg
     4981 gcatagggca ggctggaact atcaggcaag ccggggcatg atcgggcgaa cccgaacaag
     5041 gagctcaagg gacatgaacg cacgaaccgg agtcgcccac tggcacgcct atatggaaag
     5101 cggcggcgat gccgcgctgc tggacgcgct gaccgccgac gacgcggtgt tccattcgcc
     5161 cgtggtccac agcccgcagc agggcaaggc caaggtcatg ctctacctgc tcagcgccgc
     5221 caaggtgctg gggaacgaga gcttcaccta tgtccgcgaa gttatcgagg gcaacgatgc
     5281 gctgctggag ttcaccagcg agatcgacgg catccacatc aacgggatcg acctgatcac
     5341 ctttgacgat gacggtaaga tcaaggattt caaggtgatg gtccgcccgg tcaaggccgt
     5401 gaacaagctg tgggagatga tggccgcgca gcttcaggcg gcctcgggat agcgtgcatc
     5461 ggcgtggagg tagtagtcgc cgcgcccttg cggattgacc ggaatggtca gggcgctgaa
     5521 cggtgccatc agcgcccaca gcatgggggc cttgccgagc gagcagatca ccagggccgc
     5581 ggcggtcgat acgccggccc aggccccggc aaagcgcagg accgcgcgct ggagtccacg
     5641 gctgaacgcc cattcctcct cgcccagcac ttccagcagc agttcgctcg ccgccatgtg
     5701 ctgcgatgcc ggcacccaca accggtgacc gcccagcgct gccgtattca cttcggccga
     5761 tgcgtgcgca gcgccgccga catggaccag aattccgccg gcctcgagca tggaggcaac
     5821 gaccagtgcc tcgacgcggg agacaagcgc cgccacggtg accatagcat cctcgggcag
     5881 ctcatcgtcc atgaccactc ccgttcaggc cgaagggcta cgatccacca tcttgcgcag
     5941 gaattccagc cgcttgtcca ctccctcgag gggcaggtcg gtcacttcgt agccatagcg
     6001 cttccatgcc gcggtgaccg cttcgtcgct ggccacggcc tgctcccagt cctggatccg
     6061 ttcggcatcc tgggcataga tcgccgccca ggccggggcg cgcaggattg ggccggcata
     6121 gcgaagggtc cggcaggcat gatcgaccgc ggggggcacg gccagaccgg acacgtcgag
     6181 aaagcccacc acgtcgggaa agccgcgatc gaacaggact ggttcggggt gatcgccgtg
     6241 gcgttcgaat tcgcggcggt gcgcttcgag catggcttcg gcaaagccga gcgggtcgtt
     6301 ctcccgcagc gccattccgc ccggcgcctt cagcaattgg cgcgccactt cgggcgaggt
     6361 ctggataccg gccgcagcgg ccgcatccag cagcgtggtc ttgcccgccc ccggcgcacc
     6421 ggtaagaacc gcgtgccggg ggacgagcag gcccatccta ttgattcatc gtcgcaaaga
     6481 agtcttcgtt ggtcttcgaa tccttcatct tgtcgagcag gaattccatc gcgtcgacgg
     6541 tgcccatctg catcaggatc cggcgcagga cccacatctt gctgagcttg tccttctcga
     6601 ccagcagctc ttccttgcgg gtgccgctct tgccgacgtc cagcgccggg aagatgcgct
     6661 tgtcggcgac cttgcggtcg agcacgattt cgctgttgcc ggtgcccttg aactcttcga
     6721 agatcacttc gtccatgcgg ctgccggtgt cgatcagggc cgtggcgatg atcgaaaggc
     6781 tgccgccttc ctcgatgttg cgggccgcac cgaagaagcg cttggggcgc tgcagggcgt
     6841 tggcatcgac accgccggtc aggaccttgc ccgagctcgg caccacggtg ttgtaggcgc
     6901 gccccaggcg ggtgatcgaa tcgagcagga tcacgacatc gcgcttgtgc tcgaccagcc
     6961 gcttggcctt ctcgatcacc atttcggcga cctggacgtg gcgctgggcg ggctcgtcga
     7021 aggtcgagga aatcacctcg cccttcacgc tgcgctgcat gtcggtgact tcttccggac
     7081 gctcgtcgac cagcagcacc agcaggaaca cctcggggtg gttgtcggtg atcgccttgg
     7141 cgatgttctg cagcagcacg gtcttgccgg tgcgcggcgg ggcgacgatc agggcgcgct
     7201 ggcccttgcc ctgggggctg atgatgtcga tcacgcgggc cgacttgtcc ttgacggtcg
     7261 gatccagcgt atcgaggctg agcttgctgt cgggatagag cggggtcagg ttgtcgaagt
     7321 tgacccggtg gcggaccacg tcgggatcgt cgtaattgac gctgaccagc ttggtgatcg
     7381 cgaaatagcg ttcgccgtcc ttcggcgcgc ggatctcgcc ttccacggtg tcgccggtgc
     7441 gcaggcccca tttgcggacc tggttgggcg agacatagat gtcgtcggga ccggccagat
     7501 agttcgcttc ggggctgcgc aggaagccga agccgtccgc cagcacttcg atcgtgccct
     7561 ggccgatgat ttcctcgccg tcctcggcca cttccttgag gatcgcgaac atcagatcct
     7621 gccggcgcag ggtcgacgca ccttcgacgc caagctcttc ggccatttcg accagctcgg
     7681 cggaggtttt cttcttgagt tctttcagat gcatttcggg tgtcgtttcc gttgaattca
     7741 cactgggaat ggccggacga tcgatgggct tggagaaagc ggatcgcgcc gcgcttgaac
     7801 gcgctggcct tgccggtcag cgctgcagat aggcttcgcg cgctcgaacg tcaatcagaa
     7861 cgggcgcagc accaccatga tcacgatcag cgccgcggca atgccgggga cttcgttgat
     7921 catccgcagc ttgcggccgg tcagccgccg ctccccccgg gtcagcgcgg cgtgatagcc
     7981 ggccagccag atatggtagg aggtcagcgc cagcaccgcg gccagcttga cgtggaacca
     8041 gccctgcccg aacgccccgg tggcataggc cagggccagc cccagaaccc aggccaccac
     8101 cagcgagggc cacaggatga tcttgagcag ccgcgcctcg cgctcaatcc acagcgcgtt
     8161 ttccggcgaa tccggctcgg cttcctggtg gtagacaaag aaccgcggca gcatgaacag
     8221 cccggccatc cagaagatca cgaagatgat gtgcccagcc ttgagccaga ggtaggtcgt
     8281 cgagagcatc cagttcattc ccgccgctat agcctatccc tgccagccgc gcaccagggc
     8341 aagcaagtgc ccgacatgtt cgaccggggt ggtctggccg atcccgtgac cgaggttgaa
     8401 cacatgcgga cggtcggcaa aggcatcgag gatccgcagc gtctggcgct ccatctccgc
     8461 gccgccggac agcagcagca gcggatcgag gttgccctgc accggcagcc cggccggcag
     8521 cgccttggcc gcccacagcg gatcgatcgt ctcgtccagc cccagggcat cgacccgggt
     8581 ttcgcgcgca taggccggca gcttctcgcc cgcgcccttg ggaaaaccga tgatcggaac
     8641 atggggaaag cgcgcccgca gcgccttcac gatggccgcg ttgggggcaa tcacccagcg
     8701 ttcgaactcg ctgggggcga ggctgccggc ccagctgtcg aacagctgca ccgcctcggc
     8761 cccggcggca atctggccgg ccaggtattc gatggtcacc gcgacgatgg catcgatgat
     8821 cgcctggaac gcggagggat cgcggtaggc catttcgcgg gtggcgtgct ggtcgcggct
     8881 cccctcgccc gccaccatat aggtcgcgac cgtccagggg cttccggcaa agcccagcag
     8941 ggtcttgccc ggcggcaact gcgccttcac cagcttgacc gtgtcgtaga tcggggtcag
     9001 ccgttcgggc accgcgtgca ggctggccaa tgcggcatcc gccagccgcg gcgacaggtg
     9061 gggcccttcg ccaaccagaa actccagatc ctggcccatc gcatagggta cgatcaggat
     9121 gtcggaaaac aggatcgccc cgtccatgcc atagcggcgc aagggctgga tcgtgatctc
     9181 ggcggcggcc tcgctgtcat agaccagggc caggaacccg cccttttcgg ccctgagggc
     9241 gcggtattca
//
LOCUS       DASUDV010000574         4063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1146915,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000574 DASUDV010000000
VERSION     DASUDV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146915"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFV30_12745"
     CDS             <1..1200
                     /locus_tag="VFV30_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4849005.1"
                     /translation="IITALLSQPVMRRLSAESLGEALGKQAVLVETIGSLETVKSANA
                     GEMLAERWDKAVMGHAGASLRQRVVSTLSINMAGTAQTMAYTGVVIFGVFMIADHSLT
                     MGGLIACSILAGRAVAPLGAIAHLLTRINATRTAYRQINQMMQQPPEGPAGTGLAIGS
                     VQGGIEFRDVDFRYPGAPELALTKVSFRIKPGEHVALIGPIGSGKSTIARLLIGLYPP
                     TSGLVLIDGTDIRQLTPKSLRARVGALFQDNVLLTGTIRENIMLGREDVNEEEMLRAS
                     KVSGAHDFISMFPAGYDLTLADRGDGLSGGQRQAIAMARALAGKPPILVFDEPTSSVD
                     GETEARLLERLRSEFEGRTLVLITHRPSMLALVDRVILMARGRVAMDGTPQQITGRVR
                     EIAAASR"
     gene            1204..2466
                     /locus_tag="VFV30_12750"
     CDS             1204..2466
                     /locus_tag="VFV30_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX4849006.1"
                     /translation="MSSLLDHLKGDEADLDEQEFAARRSASMILWIVGAFFLLGVIWA
                     AFTELDRTVMGMGRVVPSSKMQVVSNLEGGVIQEILVKPGQMVKKGDILVRLSPTLTG
                     SELGSNEASVDALRAKVARLSAEVSGGTPNYAGVPAQQIAIEQSLHAARKAELQGLLA
                     AGNARAAQADSMLAARRSNLAAAQRELEMIEPLAAREIVSRLDLVKAQNAVTVAQSEV
                     AAAQAAASEARSATAQARSDWLSRAGLELTSAQAELNARQRAMPALSDKVDRTTIRAP
                     MTGRVNRVLVTTVGGTVSAGMPVAEIVPSDESLYIEAAVRPQDIGNVRMGQKASVEIT
                     AYRSTVYGKLDGEVISISPDAVLNEKTGESFYTVEVRTSSVLVHEGQKLPIGPGMIAN
                     VSLLGDKRSVLSYIFSPLTRVSEHAFRE"
     gene            2522..3319
                     /locus_tag="VFV30_12755"
     CDS             2522..3319
                     /locus_tag="VFV30_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4849007.1"
                     /translation="MAQQVITIEQHGAIEVVSLNRPDRLNAMNEPLIAALLDYFTGLQ
                     QRPAIRVVVLRAEGRAFCAGLDLTGWTPDPDASAISQSWRTQRMIAAVMQAMRKCPQP
                     VIAAAQGAACGGGFSLLLASDVRYAAPDLKANAAYIKIGLGGCDMGASYFLPRLVGSS
                     VASEYLLTGKFMTAEKALACGLVSEIVPVEALLERALGLAGEMLQTSPMGLRLTKDAL
                     NLNIDAASMEHAFALEDRQQVMLGQAGDFAEAKAAFFEKRPPNWADR"
     gene            complement(3320..>4063)
                     /locus_tag="VFV30_12760"
     CDS             complement(3320..>4063)
                     /locus_tag="VFV30_12760"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4849008.1"
                     /translation="VVTLNRPEAMNALSRGLRAQLAQVMVELDAEERIRAVVLTGAGN
                     RAFTAGLDLKELGSGAAGLGAATDQEATSNPVKAIETCRKPVIGAINGVAITGGFELA
                     LACDILIASENARFADTHARVGIMPGWGLSQKLSRMIGISRAKELSLTGNFIDAQTAC
                     AWGLVNRVVPADQLVPAAKALAMDVASADPAMIQAYKALIDEGYAASFGDALALELQR
                     SANANSAISAEDVENRRRAVQERGRAQQG"
BASE COUNT          695 a         1306 c         1350 g          712 t
ORIGIN      
        1 atcatcaccg ccctgctctc gcagccggtc atgcggcggc tctcggccga atcgctgggt
       61 gaggcgctcg gcaagcaggc ggtgctggtc gaaacgatcg gcagcctcga gacagtgaag
      121 tccgcgaacg ccggcgaaat gctggccgaa cgctgggaca aggcggtgat gggccatgcc
      181 ggcgcatcgc tgcgccagcg cgttgtctcg accctgtcga tcaacatggc cggcacggcc
      241 cagaccatgg cctataccgg ggtggtgatc ttcggcgttt tcatgattgc cgatcacagc
      301 ctgaccatgg gcgggctgat cgcatgctcg atcctggccg gccgcgccgt ggcaccgctt
      361 ggcgccatcg cgcacctgct tacccggatc aacgccacgc gcactgccta tcgtcagatc
      421 aaccagatga tgcagcagcc gcccgaaggg ccggcgggca cggggctggc gatcggttct
      481 gtccagggcg ggatcgagtt ccgcgatgtc gatttccgct atcccggcgc acccgaactg
      541 gcgctgacca aggtcagttt ccggatcaag ccgggcgaac atgtcgcgct gattggtccg
      601 atcggttcgg gcaaatccac catcgcgcgg ctgctgatcg ggctttatcc gcccaccagc
      661 ggactggtgc tgatcgatgg taccgatatc cggcagctga ccccgaagtc gcttcgtgca
      721 agggtggggg cgctgttcca ggataatgta ttgctgaccg gtacgatccg cgaaaacatc
      781 atgcttggcc gcgaggatgt gaacgaggag gaaatgcttc gggcctcgaa ggtcagcggg
      841 gcgcatgatt tcatttcgat gttcccggcc ggctacgacc tgacccttgc cgaccgcggc
      901 gatggacttt cgggcgggca gcgccaggcc atcgcgatgg ctcgcgcgct ggccggcaag
      961 ccgccaattc tggtgttcga cgagccgact tcttcggtcg acggggagac cgaggcccgc
     1021 ctgcttgagc ggctgcgcag cgagttcgaa ggtcgcaccc ttgtcctgat cacgcatcga
     1081 ccctcgatgc tggcgctagt cgaccgggtc atccttatgg cgcgcggccg ggtggcgatg
     1141 gatggaaccc cgcaacagat caccggacgg gtccgcgaaa tcgcggcagc atcgaggtaa
     1201 gccatgtcca gcctgctaga ccacctcaag ggtgatgaag ccgatctcga cgaacaggaa
     1261 tttgcggccc gcagatcggc cagcatgatc ctgtggatcg ttggtgcctt cttcctgctc
     1321 ggcgttatct gggcggcctt caccgaactg gaccggacgg tgatgggcat gggccgggtc
     1381 gttcccagct cgaaaatgca ggtcgtctcg aatctcgagg gcggcgtgat ccaggagatt
     1441 ctggtgaagc cgggacagat ggtgaagaag ggcgatattc tggtgcgcct ttctcccacg
     1501 ctgaccgggt ccgaactggg cagcaacgaa gcctcggtcg atgcgctgcg ggccaaggtt
     1561 gcccggctga gcgccgaagt cagcggcggc acgcccaact atgccggtgt tccggcgcag
     1621 caaatcgcga tcgagcaatc gctccacgcg gctcgcaagg cggaacttca ggggctgctc
     1681 gcagcaggca acgcccgggc tgcccaggcg gattcgatgc tcgcggcgcg gcgctcgaac
     1741 ctggctgcgg cacaacgcga gctggaaatg atcgagccgc tggccgcgcg cgaaatcgtc
     1801 tcgcggctgg atctggtcaa ggcgcagaat gccgtcaccg tcgcccagag cgaagtggcg
     1861 gccgcacagg ccgcggcaag cgaggcgcgt tccgccaccg cacaggcgcg cagcgactgg
     1921 ctgagccgtg cggggcttga actgaccagc gcccaggctg aactcaatgc ccgccaacgc
     1981 gcgatgcctg ccctgagcga caaggtcgat cgcaccacga tccgcgcgcc gatgaccggg
     2041 cgggtcaacc gcgtgctggt gaccacggtg ggcggaacgg tttcggcggg catgcccgtt
     2101 gcggaaatcg tgccgtccga tgaatcgctc tatatcgaag ccgcggtacg cccacaggat
     2161 atcggcaacg tacggatggg gcagaaggcc agcgtggaaa tcaccgccta ccgttcgacg
     2221 gtctatggca agcttgatgg cgaggtgatc tcgatttcgc ctgacgcggt tctgaacgaa
     2281 aagaccggcg aaagtttcta cacggtcgaa gtgcggacat cttcggtgct ggtccacgag
     2341 gggcagaaac tgccgatcgg gccgggcatg atcgccaatg tcagcctgct gggcgacaag
     2401 cgctcggtcc tgtcctacat cttctcgccg ctcacccgcg tttcggagca cgccttccgc
     2461 gaatgacact tgccttgtcc ctcctggcgc gttcaactgg cgcatcagga gggcagggtt
     2521 catggcgcag caggttatca cgatagagca gcacggagcg atcgaggttg tcagccttaa
     2581 ccggcctgat cgcctgaatg cgatgaacga gccgctgatt gcggcactgc tcgattattt
     2641 taccgggctc cagcagcgcc cggcgatccg ggttgtggtg ctgcgcgccg agggccgggc
     2701 gttctgcgcc gggcttgatc tgaccggctg gacgcccgat cccgatgcca gcgcgatcag
     2761 ccagtcatgg cggacccagc ggatgattgc ggcggtcatg caagcgatgc gcaagtgccc
     2821 gcagccggtc atcgccgccg cccagggcgc ggcctgcggc ggtggcttct cgctgctgct
     2881 ggcgagcgac gtgcgctatg ccgcgcccga tctcaaggcc aatgccgcct atatcaagat
     2941 cggcctgggc ggctgcgaca tgggcgccag ctatttcctg ccccgtctgg ttggcagctc
     3001 ggtggcgagc gaatatctgc tgaccggcaa gttcatgacc gccgaaaagg cactggcctg
     3061 cgggctggtg agcgaaatcg tgccggtcga agccttgctc gagcgcgccc tgggcctggc
     3121 cggtgaaatg ctgcagacct cgccgatggg gcttcggctc accaaggacg cgctcaatct
     3181 caacatcgat gccgcatcga tggagcatgc cttcgcgctg gaagaccgcc agcaggttat
     3241 gctcggccag gccggcgatt ttgccgaggc caaggccgcg tttttcgaaa agcgcccgcc
     3301 caactgggcc gatcgctagc tagccctgct gcgcgcggcc gcgctcctgc accgcgcggc
     3361 ggcggttttc gacatcttcg gcgctgatcg cgctgttggc gttggccgaa cgctgcagct
     3421 cgagcgccag ggcatcgccg aagctggcgg catagccttc gtcgatcagc gccttgtagg
     3481 cctggatcat tgccggatcg gccgaagcca catccatggc cagcgccttg gccgccggca
     3541 ccagctgatc ggccggcacc acccggttga ccaggcccca ggcgcaggcg gtctgggcat
     3601 cgatgaaatt gccggtgagc gacagttcct tggcccttga aatgccgatc atgcggctga
     3661 gtttctgcga caggccccag cccggcatga tcccgacccg cgcatgggta tcggcaaagc
     3721 gggcattctc gctggcaatc aggatgtcgc aggccagcgc cagctcgaac ccgccagtaa
     3781 tcgcaacccc gttgatcgcg ccgatcaccg gcttgcggca ggtttcgatc gccttgaccg
     3841 gattgctcgt tgcttcctga tcggtcgccg cgcccaggcc ggccgcgccc gagcccagct
     3901 ccttgaggtc gagaccggcg gtaaaggcgc ggtttcccgc tccggtcagc accacggcgc
     3961 ggatccgctc ttcggcgtcc agttcgacca tgacctgggc gagctgcgcg cgcagcccac
     4021 gcgacagggc attcatcgcc tcggggcgat tgagcgtgac cac
//
LOCUS       DASUDV010000575         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1765665,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000575 DASUDV010000000
VERSION     DASUDV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765665"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1643)
                     /gene="dacB"
                     /locus_tag="VFV30_12765"
     CDS             complement(150..1643)
                     /gene="dacB"
                     /locus_tag="VFV30_12765"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX4849009.1"
                     /translation="MRNLLIPLALLSLSAPALAQAPAAPPVAPPAAAQTLQQQVETTL
                     ATAPQGTRFGLLVVDEKGQVVVAVNPDQRFIPASNTKMFVTAAAYARMQGMDKPDERG
                     GTAVTLEAVKGRKTPDVVLIGYGDARMSGAPDCEINCLAYLADAVAAKTTKVHDVIGD
                     DTIWPDQRWVPGMSWNNIGTDSGTAVSALNLDNNELLITVSPLAEGKPPFLEIGDYYE
                     VVNEAVVGPPGSKMTLAIERPVNGNRLRVYGSIPSDSKPWTDRIGIDDPAHYAAWVLR
                     KMLVARGVKVTGTVRVRHRPVQVYDAPSPPNPVLVPPPVMAIPIAALTPPPLAEDVVT
                     INKASQNLHSQVLFRRLGDLEGTGSEDWSVHALHAVLDKAGLPRGGYDFSDGSGMSTY
                     NRVSPRAMIALLKWIDSQPWGPAWHASLPIAGKDGTLRRRFIGTPLEGNLAAKTGTLN
                     ATNALSGTFRTASGKRFTFAFFANDVPNGASALPAMEAVLLLIAAAN"
     gene            complement(1667..>2412)
                     /locus_tag="VFV30_12770"
     CDS             complement(1667..>2412)
                     /locus_tag="VFV30_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX4849010.1"
                     /translation="ASLDPATMGDLGGSLASVSALLPRVDALHAALAARHGLHILAAS
                     APCEVAGGAYVNRARLFTPGGKVGVQDKLVMTRFEREEWHVSASAPLRLFQTELGMIG
                     VTICYDSEFPLLARAQVEAGMELLLVPSCTDSAHGYWRVRLGSQARALEGQCYAVQSP
                     TVGEAPWSPAVDVNRGAAAVYGPPDRGMPADGVVAIGAMDAAQWVFAEVDLARVAELR
                     ADGGVLNARHWAEQPGGEGLPRVEVVDLR"
BASE COUNT          402 a          842 c          793 g          375 t
ORIGIN      
        1 catggtaaat cccctgtcat attcgccaaa tcggtaagcc atcgcagact agccgagccg
       61 cccggcattg ccagacagtc cgcccccgaa ccgcgcgatt gcagcacttt tgtggccgcg
      121 ccgcaacagt cgcttgtcga ttgccgtggt taattggccg ccgcgatcag cagcagcacc
      181 gcttccatcg ccggcagcgc cgaggcgccg ttgggcacgt cgttggcaaa gaaggcaaag
      241 gtgaagcgct tgccgctggc ggtgcggaac gtgcccgaca gcgcgttggt ggcgttgagc
      301 gtcccggtct tggcggcaag gttgccttcc agcggagtgc cgatgaagcg gcggcgcagc
      361 gtgccgtcct tgccggcgat cggcagcgag gcgtgccagg ccggccccca gggctggctg
      421 tcgatccatt tgagcagcgc gatcatcgcc cgcggcgaca cccggttgta agtcgacatg
      481 cccgagccat cggaaaaatc atacccgccg cgcggcagcc cggccttgtc cagcaccgcg
      541 tggagcgcgt ggaccgacca gtcctccgat cccgtccctt ccagatcgcc cagccgccgg
      601 aacaggacct gcgaatggag gttctggctg gccttgttga tcgtcaccac gtcctccgcc
      661 agcggcggcg gggtcagcgc ggcgatcggg atagccataa ccgggggcgg cacaagcacc
      721 ggattcgggg gcgaaggagc atcgtacacc tgcaccggac ggtgccgaac ccgcacggtg
      781 ccagtaactt tcaccccccg cgcgaccagc atcttgcgca gcacccaggc tgcgtaatgc
      841 gccggatcgt cgatcccgat ccggtcggtc cacggtttgg aatcggaagg gatcgagcca
      901 tagacccgca gccggttgcc gttgacgggc cgctcgatcg ccaaggtcat cttgctgccc
      961 ggcggcccga cgaccgcttc gttgaccact tcatagtagt caccgatctc gagaaagggc
     1021 ggcttgcctt cggccagtgg gctgacggta atcaacagct cgttgttgtc gagattgagc
     1081 gccgagacgg ccgtaccgct atcggtgccg atgttgttcc agctcatccc aggaacccag
     1141 cgttgatcgg gccagatggt gtcatcgccg atcacatcgt ggaccttggt cgttttggcc
     1201 gcgaccgcat cggccaggta tgcaaggcaa ttgatctcgc aatccggcgc gcccgacatc
     1261 cgggcatcgc cgtacccgat caataccaca tcgggcgtct tgcggccctt gaccgcttcc
     1321 agcgtgaccg ctgtgccccc gcgctcgtcc ggcttgtcca tgccctgcat ccgggcataa
     1381 gccgccgcgg tgacgaacat cttggtgttg gacgcgggaa tgaagcgctg gtccgggttg
     1441 accgcgacca ccacctggcc cttctcgtct accaccagca ggccgaagcg ggtgccctgc
     1501 ggggcagtgg ccagcgtggt ttcgacctgc tgctgcagcg tctgcgccgc ggccggaggg
     1561 gcgaccggcg gagccgccgg ggcctgggcc agagcggggg cggaaagcga cagcagggcg
     1621 agcgggatca ggaggttgcg catcgcccgc aacactgccc tgcccctcag cgcaagtcca
     1681 ccacctccac gcgtggcagg ccctcgccgc ccggctgctc ggcccagtgg cgcgcgttga
     1741 gcacgccgcc gtcggcccgc agctctgcca cgcgggccag atcaacctcg gcaaagaccc
     1801 attgcgcggc gtccatcgcg ccgatcgcga ccaccccgtc ggccggcatc ccccggtccg
     1861 gcgggccata gacggcggcc gcgccgcggt tgacgtcgac cgcgggagac catggtgcct
     1921 cgcccacggt gggcgactgg acggcatagc actgcccctc cagcgcgcgg gcctggctgc
     1981 ccagccgcac ccgccagtag ccgtgggcgc tgtcggtgca ggaggggacc agcagcagct
     2041 ccatcccggc ttccacctgg gcccgggcca gcagtggaaa ctcgctgtca tagcagatgg
     2101 ttacgccgat cattccaagc tcggtctgaa acagccgcag cggtgcggag gcgctgacgt
     2161 gccactcctc gcgctcaaac cgggtcatca ccagcttgtc ctgaacgccc accttgccgc
     2221 caggcgtgaa cagccgggcg cggttgacat aggccccacc tgccacctca caaggcgcgc
     2281 tggcggccag gatatgcagg ccgtgacggg cagcaagcgc cgcgtgaagg gcatcgacgc
     2341 gcgggagaag ggcactgacc gaggccagag agccacccag atcgcccatc gttgcgggat
     2401 ccaggctggc ca
//
LOCUS       DASUDV010000576         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_506686,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000576 DASUDV010000000
VERSION     DASUDV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506686"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFV30_12775"
     CDS             <1..549
                     /locus_tag="VFV30_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849011.1"
                     /translation="SGPDTLAAETDNAFTYGADDPQGLACPFGAHIRRTNPRDSKEPG
                     DPSEQVITNRHRILRRGRTYRRADTGEKGMLFACLCADIERQFEFVQQFWTNAPAFHG
                     LEHEPDPIAGADPVDPLTGARQDRVFTIPTVAGPVRLNGLSNFVQTMGGGYFFLPSRS
                     ALGWLSDVALVRSATEGDRRHG"
     gene            542..1171
                     /locus_tag="VFV30_12780"
     CDS             542..1171
                     /locus_tag="VFV30_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849012.1"
                     /translation="MADQDDTGGLEQLLPFYVNRTLDPEMRARIDAALARSSELRAAL
                     AEQQEIAGMVKTGASAWQGGAEQAAVLTIAEPAAAPQPAKAGVLAFLSPANWRPAVAL
                     GLAVAVMAQGAGLVAQQVTIARLEEENYQLASGQDTPGRKGSILIELNDDARWSAVTA
                     LLAEEGLEIVASSDLGTLTLATDKTGAEREAVLARLRASPLVASAEPAA"
     gene            1168..2421
                     /locus_tag="VFV30_12785"
     CDS             1168..2421
                     /locus_tag="VFV30_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4849013.1"
                     /translation="MKRAALALGLAALAAVPVLAQVGGPVPQTQNPDAPRSGSSGFPG
                     VGLSIRLGGKKKAPEPALTGPPLEMRDRAIPDFVPGQVLFFITGDEAAAKRIARTGRV
                     RFVRIEPLPEMGVAMVVAALQAGAPVPAAVERLGRLPGVEWAQPDFEFQMLGNSRELG
                     AGLSGIKLGPKHKVSGTIVMIDSPVDLGNPALAGARITQQGFGGAMTPSPHGTAVAEL
                     LVGTGTFAGVAQGAQLVSLAAFAPVGEDWWRSQSSLVLVAADRALALRPNVLNLSFGT
                     RRDDPALARALARFEERGTCVVAAAGNGGGGPVLFPARHPAVLAVTAIDGGKRVYTYA
                     SRGPEIDIAAWGVGMNAAVPGGRRAVSGSSFATAIVSGAMLRLPACARDRKPAGMRTS
                     VAATAQDLGPKGRDDIFGAGLMRLR"
     gene            2547..>3188
                     /locus_tag="VFV30_12790"
     CDS             2547..>3188
                     /locus_tag="VFV30_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4849014.1"
                     /translation="MEASRPRLSLLRILEMNLGFLGLQFSFGLQQANMGPIWGYLGAE
                     EADFSWLGIAGPLTGLLVQPIVGVLSDRTGGRWGRRTPYFLAGAVMCSLGLLFMPLSN
                     AVIMAFSLMFLLDVGNNVTMEPYRAYVNDRLNPDQRSWGFLSQSAFTGLAQTLAYLAP
                     SLLVGLGLSQTAEVRGEIPQFVLISFWIGAFLSISTIVWSILRVPELPLSQEEK"
BASE COUNT          437 a         1099 c         1147 g          505 t
ORIGIN      
        1 tcggggccgg acacgctcgc ggccgaaacc gacaatgcct ttacctatgg cgcggacgat
       61 ccgcagggcc tggcctgtcc gttcggcgcg cacattcgcc gcaccaaccc gcgcgacagc
      121 aaggagccgg gcgatccctc cgaacaagtc atcaccaacc gccaccggat cctgcggcgc
      181 gggcggacct accgccgggc ggacacgggc gagaagggca tgctgttcgc ttgcctgtgc
      241 gccgacatcg agcggcagtt cgaattcgtc cagcagttct ggaccaacgc cccggccttt
      301 cacgggctgg agcacgagcc cgatccgatc gccggtgccg atccggtcga cccgctgacc
      361 ggcgcgcggc aggaccgggt ctttaccatc ccgaccgtcg ccggcccggt gcggctgaac
      421 ggcctttcga atttcgtgca gaccatgggc gggggatact tcttcctgcc gagccgctcc
      481 gcgcttggct ggctgagcga cgtcgcgctg gttcgcagtg ccaccgaagg agaccgccgc
      541 catggctgat caggacgata ccggcgggct ggaacagctg ctgccgttct atgtgaaccg
      601 cacgctcgat cccgaaatgc gggcgcggat cgacgcggcc ctcgcccgct cgtccgagct
      661 gcgcgccgcc ctggccgagc agcaggagat tgccggcatg gtcaagaccg gggcaagcgc
      721 ctggcagggc ggcgcggaac aggccgccgt gctgacgatc gcggagccgg cggccgctcc
      781 gcaaccggcc aaggctggcg tgctggcctt cctctcgccc gcgaactggc ggccggctgt
      841 ggcgctgggc ctggccgtgg cggtgatggc gcagggcgcc ggtctggttg cgcagcaagt
      901 caccatcgcc cggctggagg aggagaacta ccagctcgcc tcgggccagg acacgccggg
      961 ccgcaagggc tcgatcctga tcgagctgaa cgacgatgcc cgctggagcg cggtgaccgc
     1021 gctgctggcc gaggaagggc tggagatcgt cgccagcagc gatctgggca ccctgaccct
     1081 cgccaccgac aagaccgggg ccgagcgcga ggccgtgctg gcccggctgc gcgcgtcgcc
     1141 gctggtggcc agcgcggagc ccgcggcatg aagcgcgcgg cgctggctct gggcctggcg
     1201 gccctcgcgg cggtgccggt tctggcccag gttggcgggc cggtgccgca gacgcagaac
     1261 ccggatgccc cgcgttcggg cagcagcggc tttcccgggg tgggcctgtc gatccgtctc
     1321 ggcggcaaga agaaggcgcc cgaaccggcg ctgaccggcc cgccgctgga gatgcgcgac
     1381 cgcgctattc cggatttcgt gcccggccag gtgctgttct tcatcaccgg cgatgaagcc
     1441 gcggccaagc ggatcgcccg caccggccgg gtccgctttg tccggatcga gccgctgccc
     1501 gaaatgggcg tggcgatggt tgtcgccgcg ctgcaggccg gggccccggt tcccgccgcg
     1561 gtcgagcggc tggggcggct gcccggggtg gaatgggccc agcccgattt cgaattccag
     1621 atgctgggca acagccgcga actgggcgcg gggctgagcg ggatcaagct ggggcccaag
     1681 cacaaggtat cgggcacgat cgtgatgatc gacagcccgg tcgatctggg caacccggcg
     1741 ctggccgggg cgcggatcac ccagcagggg tttggcggag ccatgacccc ctcgccccac
     1801 ggcaccgcag tggccgagct gctggtcggc accgggacct tcgccggcgt ggcgcagggc
     1861 gcacagctgg tcagcctggc cgcctttgct cctgtggggg aggactggtg gcgttcccag
     1921 tccagcctgg tgctggttgc ggccgaccgg gcgctggcgc tgcggcccaa cgtgctcaac
     1981 ctctcgttcg ggacccggcg cgacgatccg gcgctggccc gggccctcgc ccggttcgag
     2041 gagcgcggca cctgcgtggt cgccgcggcc ggaaacggcg gcggcgggcc ggtgctgttc
     2101 ccggcgcggc accccgccgt cctggccgtg accgcgatcg acggcggcaa gcgggtttac
     2161 acctatgcct cgcgcgggcc ggaaatcgac atcgcggcct ggggtgtggg gatgaacgcc
     2221 gcggtgccgg gcgggcggcg ggccgtgtcg ggcagcagct ttgccacggc aatcgtctcg
     2281 ggcgcgatgc tgcgcctgcc cgcctgcgcg cgggatcgca agcccgcggg catgcgcacc
     2341 agcgtggccg ccacggccca ggaccttggc ccgaaaggcc gcgacgacat cttcggtgcc
     2401 ggcctgatgc ggctgcgcta agctcccttc cggagccctt tcggactcgc gcgcacagat
     2461 tattgcaatc gattgttgtc gtttgccgca agctgtgtca tttagccttc cgcggacagc
     2521 gattccgcgc aagaggggag agtgccatgg aggccagccg gccgcggctt tcgctgctgc
     2581 gaatcctgga aatgaacctg ggcttcctgg gcctgcagtt cagtttcggg ctgcagcagg
     2641 ccaacatggg cccgatctgg ggctatctcg gcgccgaaga ggccgacttt tcgtggctgg
     2701 gcattgccgg gccgctgacc ggcttgctgg tccagccgat cgtcggcgtg ctgagcgacc
     2761 ggaccggcgg gcgctggggc cgccgcaccc cctatttcct cgccggcgcg gtgatgtgct
     2821 cgctcggcct gctgttcatg ccgctgtcca acgcggtgat catggccttc tcgctgatgt
     2881 tcctgctcga tgtcggcaac aacgtcacca tggagcctta ccgcgcctat gtgaatgacc
     2941 ggctcaaccc cgatcagcgc agctggggct tccttagcca gagcgccttt accggcctgg
     3001 cccagacgct ggcctacctc gccccgtcgt tgctggtcgg cctgggcctg agccagaccg
     3061 ccgaagtgcg cggcgagatt ccgcagttcg tgctgatctc gttctggatc ggggccttcc
     3121 tgtcgatctc gacgattgtc tggtcgatcc tgcgggtgcc cgaactgcca ctctcgcagg
     3181 aggaaaag
//
LOCUS       DASUDV010000577         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1058942,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000577 DASUDV010000000
VERSION     DASUDV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058942"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VFV30_12795"
     CDS             <1..120
                     /locus_tag="VFV30_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX4849015.1"
                     /translation="SVPLALDTAVRDGRIKAGDLVVLEAMGGGFTWGASLLRL"
     gene            238..525
                     /locus_tag="VFV30_12800"
     CDS             238..525
                     /locus_tag="VFV30_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX4849016.1"
                     /translation="MSTLTRADLAEALNRKLGLSRSESLAMVESILDLMSGALDKGEN
                     VKISGFGTFLLRDKAQRIGRNPKTGVEVPITPRRVLTFRASQMLKERIAKG"
     gene            522..968
                     /locus_tag="VFV30_12805"
     CDS             522..968
                     /locus_tag="VFV30_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX4849017.1"
                     /translation="MSDGDLLAGTAQGQGFADGKDPEALRTIGEVAKALGIRQHVLRY
                     WEEQFPMLKPLKRSGGRRYYRSEDVRLVETIDRLVHREGYTLKGAKKALQSGGRTVAE
                     SAPAPAAAPAATPAATPPLSAMSANFALRARLISLRTTLVNALHEA"
     gene            1000..>2023
                     /locus_tag="VFV30_12810"
     CDS             1000..>2023
                     /locus_tag="VFV30_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4849018.1"
                     /translation="MTQTYDADLALFINGSWQIGEGRDLFPVVNPATGDAIAEVPLAT
                     PADLDAALAAAETGFALWKATPVEQRAAVLRKTAALLRERVDSIATLLTLEQGKVLAE
                     ARAEVMGSAQIFDWCAEEASRIYGRVLVRPTGQRSLVTRQPVGPVATFSPWNFPVYLM
                     AKKLAPALAVGCSVIAKPPEETPGCTGALMRCLIDAGLPAGVAQLVHGVPDMVSRHLI
                     ASPVIRKISFTGSTPVGKHLMRLAADSLKRVTMELGGHAPVLIFEDCDLDKTLDMVVP
                     QKFRNAGQVCVSPTRFYVQESIYQRFVEGFAARTQGVTPGSGLDADSKMGPLANVRRP
                     GAIEALV"
BASE COUNT          335 a          684 c          680 g          324 t
ORIGIN      
        1 tcggtgccgc tggcgctgga tacggcggtg cgcgacgggc ggatcaaggc cggcgatctg
       61 gtcgtgctgg aggcgatggg cggcggcttt acctggggcg ccagcctgct gcgtctgtga
      121 agaacggcgg ataaagtgcc gcagctacgc gattgcgttg caaatcccgt acgagtttgc
      181 aattctcccg actcgagagg tcgcgttcga atatcctgaa aggtgctggt gcgatccatg
      241 tctactctga cccgagccga tctggcagaa gctctcaacc gcaagctcgg gctgtcccgc
      301 tcggaatcgc ttgcgatggt ggaatcgatt ctcgacctga tgtccggcgc gctggacaag
      361 ggtgagaacg tcaagatttc cgggtttggc acgttcctgc tgcgcgacaa ggcccagcgt
      421 atcggccgca atcccaagac cggcgtcgaa gtgccgatca ccccgcgccg ggtgctgacc
      481 ttccgcgcca gccagatgct caaagagcgg atcgccaagg gatgagcgac ggggatctcc
      541 ttgccggcac ggcgcaaggc caaggctttg ccgacggcaa ggatcccgaa gccctgcgca
      601 cgattggcga ggtggcgaag gccctgggca ttcgccagca cgtcctgcgc tattgggaag
      661 agcagttccc gatgctgaag ccgctgaagc gcagcggcgg tcggcgctat taccggtccg
      721 aggatgtccg gctggtcgag acgatcgacc ggctggtcca ccgcgaaggc tataccctga
      781 aaggggccaa gaaggccctg caaagcggcg gccgcaccgt ggccgagagc gcgcctgccc
      841 ccgcggcggc accggcggcc acccccgccg cgaccccgcc gctttcggcg atgagcgcca
      901 acttcgccct gcgggcccgg ttgatttcgc tgcggaccac actggtcaac gcgctgcacg
      961 aggcttaaag cctccctcac gagagtgagg accccttcca tgacccagac ttacgacgcc
     1021 gacctggcgc tgttcatcaa cggcagctgg cagatcggtg agggccgcga cctgttcccc
     1081 gtggtcaatc ccgccaccgg cgatgccatt gccgaagtgc cgctggccac cccggccgat
     1141 ctcgacgccg cgctggccgc cgccgaaacc ggctttgccc tgtggaaggc caccccggtc
     1201 gaacagcgcg ccgcagtgct gcgcaagacc gccgcgctgc tgcgcgagcg ggtcgattcg
     1261 atcgcgacct tgctgacgct ggaacagggc aaggtcctgg ccgaggcgcg ggccgaagtg
     1321 atgggcagcg cccagatctt tgactggtgc gccgaagagg cgagccggat ctatggccgc
     1381 gtgctggtcc gccccaccgg ccagcgctcg ctggtcaccc gccagccggt tggcccggtc
     1441 gcaaccttca gcccgtggaa tttcccggtc tacctgatgg ccaagaagct ggccccggcg
     1501 ctggccgtcg gctgttcggt gatcgccaag ccgcccgagg aaacccccgg ctgcaccggc
     1561 gcgctgatgc gctgcctgat cgacgcgggc ctgcccgcgg gcgtggccca gctggtccac
     1621 ggcgtgcccg acatggtcag ccggcatctg atcgccagcc cggtgatccg caagatcagc
     1681 ttcaccggat cgactccggt gggcaagcac ctgatgcggc tggcggcgga cagcctgaaa
     1741 cgggtgacga tggaactggg cggccacgcc ccggtgctga tcttcgagga ttgcgatctc
     1801 gacaagacgc tcgacatggt cgtgccccag aagttccgca acgccggcca ggtctgcgtc
     1861 agcccgaccc ggttctacgt ccaggaatcg atctatcagc gctttgtcga aggctttgcg
     1921 gcgcggaccc agggcgtcac cccgggctcc ggtctggatg cggacagcaa gatgggcccg
     1981 ctcgccaacg tccgccgccc cggcgcgatc gaggcgctgg tcg
//
LOCUS       DASUDV010000578         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_152738,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000578 DASUDV010000000
VERSION     DASUDV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152738"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1349)
                     /locus_tag="VFV30_12815"
     CDS             complement(141..1349)
                     /locus_tag="VFV30_12815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4849019.1"
                     /translation="MSRVGAAIRGFVNPHIPDDIRDEFVLLSASQLQAQSRLLFFALL
                     LTTPMAALAAAPDASLWVRYGVPAAMAALCLAGFLNLSRDLRLDRSVRRARRFLREAT
                     VSSSLIALMCSTWCVTSWLGAPPEQRIYYPIIIALGAFSTAYCVSSARIGAILNIAIN
                     LVPMLALLFTSGNRMDLAVGVSLLVAALFQLHMIHKSQTNVVNLLSLQRHSRNLAITD
                     PLTGLLNRRALLENALELGAQAPLRLLLVDIDNFKAINDRHGHDLGDEVLREVAQALA
                     IRADLMASVARIGGEEFAILGRADELPEALALGLLADVRAAPMPHGKPVTVSIGLAEA
                     AVDDELGWRELYRRADSALYAAKQGGRNRVVHAEPESAAPQTSAAPATGSAAPATGSA
                     AAATGSAAAA"
     gene            1516..>3321
                     /locus_tag="VFV30_12820"
     CDS             1516..>3321
                     /locus_tag="VFV30_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HEX4849020.1"
                     /translation="MPQTAPSNPTLPQNDNLIRREERKLQLDLTRLSYEWGTLDTVPQ
                     VPLASGLPLNEVPTLTWWVMLADILVGLVVNHLEVQKAAAAQDPAADTAGIAANAALA
                     AAIATEVAAIKSRIAHNTQGGTIWTRLAEDAELFVEGLGEEALAARFEDHARALCAAL
                     ELSVAKEADFGTALPRSLETYRALFQTIPLPGIAWTFQDDAEFARLRVAGPNPVLIER
                     VEQMPAKFPLDPARYAAVVAGDTLAAALAEGRLYLLDYVALEGLVPGSTDGQAKYVYC
                     PMALFAVPPGGASLVPVAIQCGQDPSVHPIFTPSVRPDEEWGWSIAKLIVQVADGNWH
                     ELFAHLAHTHLVIEAVAVATHRHLADLHPLWALLVPHCEGTLFINYEAATSLITAGGP
                     IDRIFGGTIASSQQTAAEARLNFEFTKRMLPNDLKLRGVADPACLPDYPYRDDGLLVW
                     QAIHAWVKGYVGLYYANDGAVTGDTELAAWTQDIAGTGKIRGFGPITTVAELVDTLTM
                     ILFTASAQHAAVNFPQETVMEFAPAVTGAGWQPAPLETQGHDRSDWLNYMPPSSLALK
                     QLEVLHLLGSIHYRPLGTYLSRDYPYPAWFTDPAVT"
BASE COUNT          560 a         1112 c         1140 g          509 t
ORIGIN      
        1 tcaaacgggg ggatctggcc gggggcggcg gggagcatgc gtttgtcgat cgtcatcggg
       61 gtctcgcggg cggttgggag gggcaaccgg cgatcctgac gcagacaggg ggagtaggaa
      121 aatggtttgt ggcggcgggc tcaggccgcg gcggcggagc cggtggccgc ggcggcggag
      181 ccggtggccg gcgcggcgga gccggtggcc ggcgcggcgg aggtttgcgg cgcggcgctt
      241 tccggttcgg cgtggaccac gcggttgcgg ccgccctgct tggcggcata gagcgcgctg
      301 tcggcccggc ggtaaagctc gcgccagccc agctcgtcgt ccaccgcggc ttcggccagg
      361 ccgatcgaga cggtcaccgg cttgccgtgg ggcatcggcg cggcgcggac atcggccagc
      421 aggccgagcg ccagggcctc gggcagttca tcggcgcggc ccaggatcgc gaactcctcg
      481 ccgccgatgc gggcaacgct ggccatcagg tcggcgcgga tcgccagggc ctgggccact
      541 tcgcgcagca cctcgtcgcc aaggtcatgg ccgtggcggt cgttgatcgc cttgaagttg
      601 tcgatatcca ccagcagcag ccgcagcggg gcctgggcgc ccagctccag cgcgttttcc
      661 agcagggcgc ggcggttgag caggccggtc agcggatcgg tgatcgccag gttgcggctg
      721 tggcgctgca ggctcagcag gttgaccacg ttggtctggc tcttgtggat catgtgcagc
      781 tggaacagcg cggcgaccag caggctgacc cccaccgcca ggtccatccg gttgcccgag
      841 gtgaacagca gggccagcat cggcaccagg ttgatcgcga tgttgaggat cgccccgatc
      901 cgcgcgctcg acacgcaata ggccgtactg aaggccccca gcgcgatgat gatcgggtag
      961 tagatccgct gttccggcgg cgcgcccagc cacgaggtga cgcaccaggt ggagcacatc
     1021 agcgcgatca ggctggacga gacggttgcc tcgcgcagga agcggcgggc acggcggacg
     1081 ctgcggtcaa gccgcagatc gcgcgagagg ttgaggaagc cggccaggca cagcgccgcc
     1141 atcgccgccg gcacgccgta gcgcacccac aggctcgcat cgggcgcggc ggccagcgcg
     1201 gccatcggcg tggtcagcag cagcgcgaag aacagcagcc gcgactgggc ctgcagctgg
     1261 ctggcggaca ggagcacgaa ctcatcccgg atgtcgtccg gaatgtgcgg attgacgaac
     1321 ccgcggattg ctgcccccac cctgctcatc aagcgagatt aaggcgtcac acccgaaaat
     1381 tcggttaaaa cccgcaacga attcttggac tccaagtaat tcttcaagta aaacgcaatt
     1441 caagaacaaa acaattaacg ggttggtaac attgatttga agccgttttt ccttttcaaa
     1501 acaaaggttc caaccatgcc ccagaccgcc cccagcaatc cgacgcttcc ccagaacgac
     1561 aacctgatcc gccgcgagga acgcaagctg cagcttgatc tgacccggct gagttatgag
     1621 tggggcacgc tggatacggt gccgcaggtg cccctggcga gcgggctgcc gctgaacgaa
     1681 gtgccgacgc tgacctggtg ggtgatgctc gccgacatcc tggtcggcct ggtcgtgaac
     1741 catctggaag tgcagaaagc cgccgccgcg caggatccgg cggccgatac ggccggcatc
     1801 gccgccaacg ccgccctggc cgcagcgatc gctaccgaag tcgccgcgat caagtccagg
     1861 atcgcccaca acacgcaagg cggcacgatc tggacgcgcc tggccgagga tgccgagctg
     1921 ttcgtggaag gactgggcga agaggccctc gccgcccggt tcgaagacca tgcccgcgcg
     1981 ctctgcgccg cgctggaact gagcgtggcg aaggaggccg atttcggcac cgccctgccc
     2041 cgcagcctgg agacctaccg cgcgctgttc cagaccatcc cgctgcccgg gatcgcctgg
     2101 acctttcagg acgacgccga attcgcccgg ctgcgggtcg ccgggcccaa cccggtgctg
     2161 atcgagcggg tcgagcagat gccggccaag ttcccgctcg atccggcgcg ctatgccgca
     2221 gtggtcgccg gcgatacgct ggcagcggcg ctcgccgaag ggcggcttta cctgctcgac
     2281 tatgtggcgc tcgagggtct ggtccccggc tccaccgacg ggcaggccaa atacgtctat
     2341 tgcccgatgg cgctgttcgc cgtgccgccg ggcggcgctt cgctggtgcc ggtggcgatc
     2401 cagtgcgggc aggacccgtc ggtgcacccg atcttcaccc cctcggtccg cccggacgag
     2461 gaatggggct ggagcattgc caagctgatc gtgcaggtcg ctgatggcaa ctggcacgag
     2521 ctgttcgccc accttgccca cacgcacctg gtgatcgagg ccgtggcggt ggcaacccac
     2581 cgccaccttg ccgatctcca cccgctgtgg gcgctgctgg tgccgcattg cgaaggcacg
     2641 ctgttcatca attacgaggc ggcgaccagc ctgatcaccg ccggcggacc gatcgaccgg
     2701 atctttggcg ggacgatcgc ctccagccag cagaccgcgg ccgaggcccg gctgaacttc
     2761 gaattcacca agcggatgct gcccaacgat ctgaagctgc gcggcgtggc cgatcccgcc
     2821 tgtctgccgg attaccccta ccgcgacgat ggcctgctgg tgtggcaggc gatccacgcc
     2881 tgggtgaagg gctatgtcgg gctctattac gccaacgacg gcgccgtcac cggcgacacc
     2941 gaactggcgg cgtggaccca ggatatcgcc ggtacgggca agatccgcgg gttcggcccg
     3001 atcaccacgg tggccgagct ggtcgatacg ctgaccatga tcctgttcac cgccagcgcc
     3061 cagcacgccg cggtcaattt cccgcaggaa accgtgatgg aattcgcccc ggcggtgacg
     3121 ggggccggct ggcagccggc cccgctggaa acgcagggtc acgaccggag cgactggctg
     3181 aactacatgc cgccctccag cctggcgctg aagcagctgg aagtgctcca cctgctcggc
     3241 tcgatccact accgcccgct cggcacctat ctcagccgcg actatcccta cccggcgtgg
     3301 tttaccgatc cggccgtgac g
//
LOCUS       DASUDV010000579         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_263108,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000579 DASUDV010000000
VERSION     DASUDV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263108"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1122
                     /locus_tag="VFV30_12825"
     CDS             67..1122
                     /locus_tag="VFV30_12825"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX4849021.1"
                     /translation="MIPPGETIGILGGGQLGRMLAIAAAQLGYRTHVFAPEPDSIAAE
                     VCGAFTNARWDDGAAMAAFAADCAVVTYEFENVPVGPLAKLGETALLPNARALEVAQD
                     RLSEKQFVEQLGGRTAPYHVVDTREDLDEALVTIGSPGILKTRREGYDGKGQWRIMTD
                     HEAEGIAIGSQALLFEGFVHFSAEFSVILARGADGAIAFWDSAENVHKGGILDTSSLP
                     ASGEIQAQVPAARELAARVADALGYVGVLTLEFFATAQGPVFNEMAPRVHNSGHWSIE
                     GALTSQFENHIRAICGLPLGSTALTAPAVEMKNLIGDAAHDWPAILADPRNHLHLYGK
                     AAARPGRKMGHVTRLGL"
     gene            1119..1610
                     /locus_tag="VFV30_12830"
     CDS             1119..1610
                     /locus_tag="VFV30_12830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX4849022.1"
                     /translation="MNRSEVFIAVAVADNGVIGNAGALPWHIPADLRRFKALTMGMPM
                     VMGRKTFESLPGLLPGRRHIVLTRDTSWSEDGAEVAHSLEDALGLANSPHIAVVGGAE
                     IYAMFLHRADRIELTEIHASPAGDTVFPHFDPSEWRETFREAHGAEGKLPPFDFVTLV
                     RKS"
     gene            1632..>2656
                     /locus_tag="VFV30_12835"
     CDS             1632..>2656
                     /locus_tag="VFV30_12835"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX4849023.1"
                     /translation="MRKRIWIPLAVLGVAALGFFGVGPGYLESNMNKLDGKEQLAVSA
                     EAQALHQTLAIVDLHSDTLMWQRDLLERASRGHEDLPRLQQGNVALQVFSSVTKTPRG
                     QNYDSNPATTDNITPLVIAQRQPLRTWTSLLERSLFHAQKLDSAVAGSQGALAKVADP
                     QGLDALLAARAAGKGPVGALLSIEGLQNLEGKLENLDKLHAAGFRMAGLAHFFDNDVA
                     GSMHGEKKGGLTPLGRQVVARMESIGMIVDIAHCSRTCVAEVLAMARRPVVSSHGGVQ
                     AMCKVNRNLTDEEVRGVARTGGVVGIGYWDGAVCDNSPRSIARSIKYVRDLVGIQHVA
                     LGSDYDGA"
BASE COUNT          460 a          867 c          894 g          435 t
ORIGIN      
        1 cttccgcgcc gcgcagaccg atagcgtcgc ccagcgtccc tcctgaacgg aaacccgaac
       61 agcaccatga tccctcccgg cgaaaccatc ggcatccttg gcggcgggca gctgggccgg
      121 atgctggcga tcgcggcagc ccaattgggc taccgcaccc atgtcttcgc gcccgagccg
      181 gattcgatcg cggccgaggt ctgcggcgcc ttcaccaatg cccggtggga cgatggcgcg
      241 gcaatggccg ccttcgccgc cgactgcgcg gtggtgacct acgagttcga gaacgtaccg
      301 gtcggcccgc tggccaagct gggcgaaacc gcactgctgc ccaatgcccg tgcgcttgaa
      361 gtggcgcagg atcggctgag cgaaaagcag ttcgtcgagc agctgggcgg ccgcaccgcg
      421 ccctaccacg tggtcgatac ccgcgaggat ctcgacgagg cgctggtgac gatcggatcg
      481 cccggcatcc tcaagacccg gcgcgaaggc tatgacggca agggccagtg gcggatcatg
      541 accgaccacg aggccgaagg tatcgcgatt ggtagccagg cgctgctgtt cgagggcttc
      601 gtgcacttct cggccgagtt ctcggtgatc ctggcgcgcg gcgccgatgg cgcgatcgcc
      661 ttctgggaca gcgccgagaa cgtccacaag ggcgggatcc tcgatacttc gagccttccg
      721 gcatccggcg aaatccaggc ccaggtccct gccgcgcgcg agctggcggc acgtgttgcc
      781 gacgcgctgg gctatgtcgg ggtgctgacg cttgaattct tcgccaccgc gcaaggcccg
      841 gtgttcaacg aaatggcacc ccgcgtgcac aattcgggcc actggtccat cgaaggggcg
      901 ctgaccagcc agttcgaaaa ccacatccgc gcgatctgcg gcctgccgct gggctccacc
      961 gcgctgaccg cgccggcggt ggagatgaag aacctgatcg gcgatgccgc gcacgattgg
     1021 ccggcgatcc tggccgatcc gcgcaatcac ctgcacctct atggcaaggc cgccgcgcgg
     1081 cccggtcgca agatgggcca tgtaacgagg ctgggacttt gaaccgttcg gaggttttca
     1141 tcgccgtggc cgtggccgac aacggcgtga tcggcaatgc cggcgccctg ccctggcaca
     1201 tcccggcaga ccttcgccgg ttcaaggcgc tgaccatggg catgccgatg gtgatgggcc
     1261 gcaagacctt cgaatcgctg cccggcctgc tgccaggccg gcgccacatc gtgctgaccc
     1321 gcgataccag ctggagcgag gacggcgccg aggttgccca ttcgctcgag gatgcgctgg
     1381 gccttgccaa ttcaccgcac atcgcggtgg tcggcggcgc ggaaatctat gccatgttcc
     1441 tgcaccgggc cgaccggatc gaattgaccg agattcacgc ctcgccggca ggcgacaccg
     1501 tgtttccgca cttcgaccca agcgaatggc gcgagacctt ccgcgaggcc catggcgccg
     1561 aaggcaaact tccccccttt gacttcgtaa ctctggtgcg taagtcctag ccagattgag
     1621 ggaggggcac gatgcgcaag cggatctgga ttccattggc ggtgctgggc gtggcggcgc
     1681 ttggcttttt cggggtaggc ccgggctatc tcgaaagcaa catgaacaag cttgacggca
     1741 aggagcagct ggcggtgagc gccgaggcgc aggccctgca ccagacgctg gcgattgtcg
     1801 atctgcattc cgacaccctg atgtggcagc gcgacctgct tgaacgggcc agccgcggac
     1861 acgaagatct gccgcggctg caacagggca atgtcgcgct gcaggtattc tcgagcgtga
     1921 ccaagacccc gcgcggccag aactatgaca gcaatcccgc cacgaccgac aacatcaccc
     1981 cgctggtgat cgcccagcgc cagccgctgc ggacctggac ctcgctgctc gaacgttcgc
     2041 tgttccatgc ccagaagctg gatagcgcgg tggccggatc gcaaggcgcc ctggcaaagg
     2101 ttgccgatcc gcaagggctt gatgcgctgc tggccgcgcg cgccgcgggc aagggtccgg
     2161 tcggcgcgct gctttcgatc gaagggctgc agaaccttga gggcaagctc gaaaacctcg
     2221 acaagctcca cgcggcgggt ttccgcatgg cgggcctggc gcatttcttc gacaacgatg
     2281 tcgccggttc gatgcatggc gagaaaaagg gcgggctgac cccgctcggc cgccaggtgg
     2341 tggcgcggat ggaatcgatc gggatgatcg tcgacatcgc ccattgcagc cgcacctgcg
     2401 tcgccgaagt gctggccatg gcgcggcgtc cggtggtttc cagccacggc ggggttcagg
     2461 caatgtgcaa ggtcaatcgc aacctgaccg atgaagaggt gcgcggcgtt gcccgcaccg
     2521 gcggggtggt cgggatcggg tactgggacg gcgcggtctg cgacaattcg ccgcgctcga
     2581 tcgcccggtc gatcaaatat gtccgcgatc tggtgggaat ccagcacgtt gccctgggca
     2641 gcgactatga cggggc
//
LOCUS       DASUDV010000580         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2141357,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000580 DASUDV010000000
VERSION     DASUDV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141357"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFV30_12840"
     CDS             <1..313
                     /locus_tag="VFV30_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4849024.1"
                     /translation="NSTKGAVRLFTKGCAKELAAARIRVNSVHPGVIDTDMGRHLIDD
                     FAAAQGLGDNEVLANVSAMHPLGHLGEPQNVADAVVFLASDRSAFSTGSELVVDGGLT
                     A"
     gene            complement(321..662)
                     /locus_tag="VFV30_12845"
     CDS             complement(321..662)
                     /locus_tag="VFV30_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849025.1"
                     /translation="MTLFRLFLLACLIAIASYTSVTIASHGWNLLPVFFGDMAAMAWP
                     GQFNLDFMCFLALTAIWVSWRHQFSAAGLGLGVLAFFGGMLFLSVYLLVQSGRARGDA
                     RVLLLGEARAA"
     gene            727..1281
                     /locus_tag="VFV30_12850"
     CDS             727..1281
                     /locus_tag="VFV30_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4849026.1"
                     /translation="MNVRDEQRDKVTERLAAHLLETGLARTSLRQLAAAAGVSDRMLL
                     YYFADKAEVLTAVMARVAGELTVRLAAAIPEGEPLGPGELVRRAASFTTGTEMRRFMR
                     LWVEVVAAAAKGEQPFVAIASQIMAGFRAWVDSRLALPPGADREAMAAAIIAVVDGLA
                     LVDICSDDVVTQRMPAALAILLRD"
     gene            complement(1283..2038)
                     /locus_tag="VFV30_12855"
     CDS             complement(1283..2038)
                     /locus_tag="VFV30_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4849027.1"
                     /translation="MDCEITIYLHGVPGSPAELALARTAAPGWFVPDRNEPALGTSLD
                     DRLAELARRIGALHPDAKLHLVGFSLGAMIALRLAPLLGARVSRIDLISAAAPLEGGD
                     FLPDMAGRAVFGLARRAPGLFALLARAQSCLARIAPRWLTRQLFATAQGEDLALARSE
                     AFVDGIAAILRGGLGRSSLGYRAEIAGYVQPWADRLAQVSAPVTLWQGSADNWTPPEM
                     AEYLAQRLPNVVAVHRLEGRSHYSALGAALARL"
     gene            complement(2119..>2748)
                     /locus_tag="VFV30_12860"
     CDS             complement(2119..>2748)
                     /locus_tag="VFV30_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4849028.1"
                     /translation="EGKRLVFAADRAPQALDGVEQRLLSRLSMGLVADIQPADIELRR
                     AILEHRLQRFSPTHVPGDVVEFLARTINRNVRELVGGLNKLIAYAQLTGQAVSLQLAE
                     EQLTDILSANRRRITIDEIQRTVCQFYRVDRTEMASKRRARAVVRPRQVAMYLAKVLT
                     PRSYPEIGRKFGGRDHSTVIHAVRLIEDLRARDADMDGDVRSLLRQLES"
BASE COUNT          494 a          894 c          959 g          401 t
ORIGIN      
        1 caattcgacc aagggcgcgg tccgtctgtt caccaagggc tgcgccaagg agctggccgc
       61 ggcgcggatc cgcgtcaact cggtccaccc cggggtgatc gataccgaca tgggtcgcca
      121 cctgatcgac gatttcgcgg cagcgcaggg gctgggcgac aacgaggtgc tggccaacgt
      181 ttcggcgatg catcccctgg gccatctggg cgaaccgcag aacgtggcgg atgcggtggt
      241 gttcctggcg agcgaccgtt cggcgttcag caccggcagc gaactggtgg tcgatggcgg
      301 gctgaccgcc tgaggccggt tcaggccgcg cgcgcctcgc ccagcagcag caccctggca
      361 tcgccccgcg cccggccgct ctgcaccagc aggtagaccg acaggaacag catgccgccg
      421 aaaaaggcca gtacgccaag gccaaggccc gcagcgctga actggtggcg ccaggacacc
      481 cagattgccg taagcgcaag gaaacacatg aaatcgaggt tgaactgccc tggccaggcc
      541 attgcggcca tatcgccgaa gaacaccggc aggaggttcc aaccgtggct ggcgatagtg
      601 acgctggtgt agctggcgat cgcgatcagg caggcaagca ggaacaggcg aaacagggtc
      661 atcggtacga ctccttgtag caactgctac atggtatctt gatgtagtga ctgctacaag
      721 ggcagaatga acgtcaggga tgaacagcgc gacaaggtga ccgagcggct ggccgcacac
      781 cttctggaaa cgggcctggc gcgcaccagc ctgcgccagc tggccgcggc cgccggggtg
      841 agtgaccgca tgctgctcta ctactttgcc gacaaggccg aggttctgac tgcggtgatg
      901 gcgcgcgtgg cgggcgaact gaccgtgcgg ttggccgcgg cgatccccga gggcgaaccg
      961 ctcggccccg gcgaactggt gcgccgtgcc gcgtccttca ccaccgggac ggaaatgcgc
     1021 cgcttcatgc gcctgtgggt cgaggtggtg gctgccgccg ccaagggcga gcagcccttt
     1081 gttgccatcg ccagccagat catggccggc ttcagggcct gggtcgattc gcggctcgcg
     1141 ctgccccccg gtgccgaccg cgaagcaatg gcagcggcga tcatcgccgt ggtcgatggg
     1201 cttgccctgg tcgacatctg cagcgacgat gtggtcaccc aaaggatgcc agcggcgctg
     1261 gcgatactgc tcagggattg agctagaggc gcgccagtgc cgcgcccagc gccgaatagt
     1321 gcgagcggcc ttcaagccgg tgcaccgcca ccacattggg caagcgctgc gcgaggtact
     1381 cggccatttc gggcggggtc cagttgtccg cgctgccctg ccacaaggtg accggcgcgc
     1441 tgacctgggc cagccgatcg gcccatggct ggacatagcc ggcgatctcg gcccggtagc
     1501 ccaggcttga gcggcccagt ccaccgcgca ggatcgcggc gatcccgtcc acaaaggctt
     1561 cggaccgggc cagcgccaag tcctcgccct gtgccgtggc gaacagctgc cgcgtcagcc
     1621 agcgcggcgc gatgcgggca aggcaggatt gcgcgcgggc cagcagcgcg aacagcccgg
     1681 gcgcgcgccg cgcgaggccg aacaccgccc ggcccgccat gtccggcagg aagtccccgc
     1741 cttcgagcgg cgcggctgcg gaaatcagat cgatccgcga cacccgcgcg cccagcagcg
     1801 gcgccagccg cagagcaatc atcgcgccca gcgaaaaccc gaccagatgc agcttggcat
     1861 cggggtgaag cgccccaatg cggcgcgcca gttcggccag acgatcatca aggctggtac
     1921 caagggcggg ctcgttgcga tcgggaacga accagccggg cgccgcagtg cgcgccagcg
     1981 caagctcggc tggcgatcct ggcacgccat ggaggtagat ggtgatttca caatccatcg
     2041 cgcatcaatc cggagcggct ggggacaagc cggtgtaaaa ccgttgtaca gcgcggggac
     2101 gggcggtgga aggcggtgtc agctttcgag ctggcgcagc aggctgcgca cgtcgccatc
     2161 catatcggca tcgcgggcgc gcaggtcttc gatcaggcgc accgcgtgga tcacggtgga
     2221 atgatcgcgc ccgccgaact tgcggccgat ttccggatag gaacgcgggg tcagcacctt
     2281 ggcgaggtac atggccacct ggcgcggacg caccaccgcg cgggcgcggc gcttcgatgc
     2341 catttcggtc cggtcgaccc ggtagaactg gcacacggtg cgctggatct cgtcgatcgt
     2401 gatccgacgg cggttggccg aaaggatatc ggtaagctgt tcctcggcca gttggagcga
     2461 aaccgcctgc ccggtgagct gggcataggc gatcagcttg ttgaggccgc caaccagctc
     2521 gcgcacattg cggttgatcg tgcgggcgag gaattcgacc acgtcacccg gcacatgagt
     2581 gggtgaaaag cgctgaaggc gatgctcgag gatcgcgcgg cgcagctcga tatcggcggg
     2641 ctggatatcg gccaccagac ccatcgacag gcggctgagc aggcgctgct ccaccccgtc
     2701 gagcgcctgg ggcgcacgat cggcggcgaa gaccaaccgc ttgccttc
//
LOCUS       DASUDV010000581         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_948439,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000581 DASUDV010000000
VERSION     DASUDV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948439"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFV30_12865"
     CDS             <1..331
                     /locus_tag="VFV30_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050053 - levansucrase activity [Evidence
                     IEA];
                     GO_process: GO:0009758 - carbohydrate utilization
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 68 protein"
                     /protein_id="HEX4849029.1"
                     /translation="QSGTFAPGLRQAPNGLYGMVADCLFGPYRPLNESGLVLCNPPQE
                     PLQTYSWFVSGDLKVSSFIDFWGLKGQPVPGEPAAARQYFGGVPAPLLQLQAIADRCT
                     LAQPALA"
     gene            328..1224
                     /locus_tag="VFV30_12870"
     CDS             328..1224
                     /locus_tag="VFV30_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX4849030.1"
                     /translation="MSNQDSALLGGIEAGGTKFVLAVGPSPDRITARHAIPTRSPAET
                     LAEAAAWFAAQQPIRALGIASFGPVELDTSCPNWGRITNTPKAGWAQCDIVGHFARKL
                     GVPVGFDTDVNGAALAEHVLGAGKGRSSLAYVTVGTGIGGGLVSEGRVIHGAAHPEIG
                     HVYPRRAPGDDHFAGLCPHHGDCLEGLASGLAIAARWGASLSQLPADHPAHGIVADYL
                     GQLCHTVFATTASEIVVLGGGVMQTPGLIERVRQRCAELGAGYLPASARHQIVLPGLD
                     QNSGICGALLLGANNLEQTAAP"
     gene            complement(1244..2044)
                     /locus_tag="VFV30_12875"
     CDS             complement(1244..2044)
                     /locus_tag="VFV30_12875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4849031.1"
                     /translation="MNPDPVIVGGREIPAEAIAAEAQYHPAGDAVQAWRAAAEALAVR
                     QLLLDEAARLELTAQDRLDAEGRPLTGDDALIDALLEREVQTPTADEQTCRRFYEANR
                     ARFASSPLVEAAHILIEADPDDSFAMGLATGDARTLIRQLQADPSCFADLARARSACP
                     SREQGGNLGQVGPGQMVRPFEEALFALPENCLCLQPVKTRFGVHVIRSGRRVEARQLP
                     FEAVHQAIADYLEEASYRRASAQYIAILAAQAGVSGVSLGAADGAMVQ"
     gene            complement(2041..>2439)
                     /gene="narI"
                     /locus_tag="VFV30_12880"
     CDS             complement(2041..>2439)
                     /gene="narI"
                     /locus_tag="VFV30_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX4849032.1"
                     /translation="GVIAIIGATLLIHRRFFDPRVRAASTFSDNMVILLLWVQLALGL
                     ATIPVSAQHLDGQEMVKFMQWAQGIFTFRAGASDLIRDVAPVFKLHLFLGLTILLLFP
                     FTRLVHMLSAPVRYVWRPGYQVVRSRNLAS"
BASE COUNT          399 a          821 c          825 g          394 t
ORIGIN      
        1 gcaatcgggc actttcgcac ccggactgcg ccaggctccg aacggacttt acggaatggt
       61 cgccgactgt ctgtttggcc cctatcgccc gcttaacgaa agcggcctgg tgctctgcaa
      121 tccgccgcaa gaaccgctgc agacctacag ctggttcgtc agcggggacc tcaaggtttc
      181 aagcttcatc gacttctggg gcctgaaggg acagccggtg ccaggcgaac cggccgcggc
      241 ccggcagtac ttcggcggcg tgcccgcgcc gctgttgcag ctgcaggcca ttgcggatcg
      301 ctgcactctg gcgcaacctg cgctggcatg agcaaccagg acagcgcctt gctgggggga
      361 atcgaagccg gcggcaccaa gttcgtgctt gccgtggggc cttcgcccga ccggatcacc
      421 gcgcgccatg cgatccccac ccgcagcccg gccgaaaccc ttgccgaagc cgcggcgtgg
      481 tttgctgcgc agcagccgat acgggcgctg ggcattgcca gtttcggccc ggtggagctt
      541 gataccagtt gcccgaattg gggccggatc accaacaccc ccaaagctgg ctgggcgcag
      601 tgcgatatcg tcggccactt tgcccggaaa cttggcgtgc cggtcggatt cgataccgat
      661 gtcaacgggg cggcgctggc cgaacatgtg ctgggcgccg gcaagggccg ctcctcgctg
      721 gcctatgtca ccgtgggcac cgggatcggc ggcgggctgg tttcggaagg ccgggtcatc
      781 cacggcgccg cccatcccga aatcggccat gtctatccgc gccgggcacc gggcgacgat
      841 cacttcgccg gcctttgccc gcaccacggc gattgcctgg aaggcctggc cagcggcctg
      901 gcgatcgcgg cgcgctgggg tgcttcgctt tcccaattgc cggcagatca tcctgcccat
      961 gggattgtgg ccgattatct tggccagctg tgccacacgg tctttgccac taccgccagc
     1021 gagattgtcg tgctgggcgg cggggtgatg cagacgcccg gcctgattga acgggtgcgc
     1081 cagcgctgcg ccgagcttgg cgcgggctat ctgcccgcca gtgcaaggca ccagatcgtg
     1141 cttcccgggc tcgatcagaa cagcgggatc tgcggcgccc tgctgctggg agcgaacaac
     1201 ctggagcaga cggccgcgcc ctgaccggca gcgaagccct ggcctattgc accatggccc
     1261 cgtcggccgc gcccaggctc acaccgctga ccccggcttg cgctgcgagg atcgcgatat
     1321 attgcgcgct tgcccggcga tagctcgcct cttcgagata gtccgcgatc gcctggtgga
     1381 ccgcctcgaa aggcagttgc cgcgcttcca cccgccggcc cgaacggatc acgtgaaccc
     1441 cgaagcgggt tttcaccggc tgcaggcaaa ggcagttttc gggcaaggca aacagggctt
     1501 cctcgaacgg cctgaccatc tggcccggcc cgacctggcc aagattgccg ccctgttcgc
     1561 gcgacgggca ggccgagcgg gcccgcgcca ggtcggcaaa gcacgatgga tcggcctgca
     1621 attggcggat cagcgttcgg gcatcgccgg tggcgaggcc catcgcgaag ctatcgtccg
     1681 gatcggcctc gatcaggata tgcgcggctt cgaccagcgg gcttgaggcg aagcgcgcgc
     1741 ggttggcctc gtagaagcgc cggcaggtct gttcgtcggc ggtgggggtc tgcacctcgc
     1801 gctcgagcag ggcgtcgatc agcgcgtcat cgccggtcag cggtcggccc tcggcatcga
     1861 gccggtcttg cgcggtgagc tcaagccggg ccgcctcgtc cagcagcagc tggcgcaccg
     1921 ccagggcttc ggccgcggcg cgccaggcct gcaccgcatc gccggcaggg tgatactgtg
     1981 cctcggccgc aatcgcctcg gccgggattt cgcgtccgcc aacgatcacc ggatcggggt
     2041 tcatgacgcc aggttccgcg accgcaccac ctgatatccg ggccgccaga catagcgtac
     2101 cggggcgctg agcatgtgga ccaggcgggt gaaggggaac agcaacagga tcgtcagccc
     2161 caggaacagg tggagcttga acaccggggc gacatcgcga atgagatcgg acgcgccggc
     2221 gcggaaggtg aagatccctt gcgcccactg catgaacttg accatttcct gcccgtcaag
     2281 atgctgcgcc gaaaccggaa tggtcgccag gcccagcgcc agctgcaccc acaagagcag
     2341 gatcaccatg ttgtcgctga aggtggaggc cgcgcgcacc ctggggtcga agaaccgacg
     2401 gtggatcagg agagtggcgc cgatgatcgc gatcacccc
//
LOCUS       DASUDV010000582         7104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1876412,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000582 DASUDV010000000
VERSION     DASUDV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876412"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFV30_12885"
     CDS             complement(<1..1098)
                     /locus_tag="VFV30_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4849033.1"
                     /translation="MTEPARHPDVQPSASDIRLVIGASSAGTVFEWYDFFIYGTLAAI
                     IGKAFVPSDNALVRELVTWAGFAVGFGFRPLGAILFGYLGDKLGRKYTFLVTVTLMGV
                     ATAGVGLVPSVESIGLWAPAIIIFLRVLQGLALGGEYGGAAIYVSEHSPPNKRGFYTG
                     FIQASVVGGFVLSLAVVLGCKAMFDDATWAEWGWRVPFLLSILLLAISLWMRVKLSES
                     PVYRAMKEEGQIAGNPFVESFTYPGNKKRIFVALFGVAVGLTVIWYTATFSSLDFLVK
                     HMRVQEGVAQLIVAGSALFGLVFFVGAGALSDRVGRKPPIVWGYVATLLFLFPVFWSF
                     EWALASAHAASPEGVVVPDTLATIKIVAALTA"
     gene            complement(1148..1570)
                     /locus_tag="VFV30_12890"
     CDS             complement(1148..1570)
                     /locus_tag="VFV30_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX4849034.1"
                     /translation="MSSLARLYTPDVLGLATALARYPLDDGLRFRGQSRSQTCGSSID
                     LGLDLDSEGRICRVGLRAHACAIGQAAAAIFCQAAIGRTRTELGQACGAIEAWLAGKS
                     DLPAWPGLAAIAAARDFPARHGAITLAWKAGIEALPTS"
     gene            complement(1573..2109)
                     /locus_tag="VFV30_12895"
     CDS             complement(1573..2109)
                     /locus_tag="VFV30_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX4849035.1"
                     /translation="MTGFDIAVLLIVGLGAITGFARGFVQEIMALAAWVLALVTIHYM
                     HTPLSAALVAYVGTESGAAVLAFALLLLVPYAIVKLLSNRLGEASRNSVLGPIDRVLG
                     FGFGGVKGVVIAVMTFSVFVLGYDTVWGVGGRPTWITQARSYPFINASSEQLVSMIAE
                     RRHAAAAAESERLKKKKD"
     gene            complement(2119..3486)
                     /gene="radA"
                     /locus_tag="VFV30_12900"
     CDS             complement(2119..3486)
                     /gene="radA"
                     /locus_tag="VFV30_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX4849036.1"
                     /translation="MAKPKRRYVCQACGSIAQRWQGQCADCGEWNTLAEEAPETVFSA
                     RHDLSSGGRAIAFESLDAPGEKLRRRSTGLAEFDRALGGGLVPGSAVLMGGDPGIGKS
                     TLLLQAAAKIAAGGGDAVYISGEEAAGQVRLRAERLGLAGAPIRLAAATSVRDILTTL
                     GQMKAPALLVIDSIQTMHSDQIEGAPGTVSQVRASAFELIRYAKESGVALVLVGHVTK
                     DGTIAGPRVLEHMVDVVMSFEGERSHQYRVLRCIKNRFGPVDEIGVFAMEGQGLAEVG
                     NPSMLFLSGRDQPVAGSSVFPAIEGTRPVLVEIQALIVRLASGATPRRAVVGWDSGRL
                     AMLLAVLESRCGLNFSSAEVYLNVAGGYRLTDPAADLAVAAALVSALADKPLSADTIW
                     FGEVSLAGEIRPVAHGPIRLREAAKLGFSQAFGPGDSGRHEKEIKVSTLSMLPNLVDR
                     IMGGA"
     gene            3558..5879
                     /locus_tag="VFV30_12905"
     CDS             3558..5879
                     /locus_tag="VFV30_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HEX4849037.1"
                     /translation="MRQKELRIALVCYGGVSLAVYMHGVTKELWKLARASRAFLEGER
                     PEHGTEAVYAQLLQRIEALFNLRLRVLPDIVAGASAGGINGIFLAQAIHSGQSLEPLT
                     ALWLERADVDVLLDPDARPWSRFAKFWAMPLVWFMLKRPGNMVSASVAPETRAEVRAK
                     ISRLIRSRWFEAPFGGLGFSRLLTEALDAMASGGSEKPLLPPGHPLDLFVTATDFRGH
                     EEIVRLHSPEKIAESEHRLSIGFRALTPSEPGNRLAPLPELVFAARGTASFPGAFPAL
                     QLAEIDTLMHERGEHWASRTAFVERIMPEHSHRGDAEKVALIDGSVLVNAPFVEAMSV
                     LSDRPAQREIDRRFVYIDPKPDEIGGQRHSDPRAPGFFSVIFGSLSSIPREQPIRDNL
                     EVIAQRSRRLSTLKGIIDSLRVEVEQTVEEVFGRTLFLNRPTVDRLEAWRSKAQQTAA
                     ERAGYAFEAYGQVKYRGILQDLAGTILAAAPELMLPSADPIADHLAAYLEQHGIDRIA
                     GRKGGASPEAIAFLRDHDISFRIRRLRLLARRLSEDWQDLDADLGAAREKARDAVYRA
                     LSLYFDRQARSALGSDFGAIARLVLDRPGHVLDTIAQRRQLPATDLVVDAMLAEAMAE
                     MPRALRRKVLLTYLGFPFYDTVTLQLLRGEGSSELEPAKVDRISPEDCNSIRDGGAET
                     TLRGTEFYNFGAFFSRAYRENDYLWGRLHGVERMIDLLVSALPPGFELDAQELAQFKR
                     RGFETVLDEEQDRLVTDPNLVPSIRAELHRLEN"
     gene            complement(5876..7012)
                     /gene="dnaJ"
                     /locus_tag="VFV30_12910"
     CDS             complement(5876..7012)
                     /gene="dnaJ"
                     /locus_tag="VFV30_12910"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX4849038.1"
                     /translation="MAVVADYYELLEVERGADDATIKSSFRKLAMRWHPDKNPGDAEA
                     EGRFKAINEAYDCLKDPQKRAAYDRFGHEAFKQGMSGGGGGGAGMGGGFSDLGDIFET
                     IFGSAFGGGGRQQARRGADMRYDLEIGLDDAFHGKQAEIEIEVASKCEPCDGSGAEPG
                     TGTRRCNLCGGHGQVRAQQGFFMVERTCPTCHGRGEVIEKPCRSCRGEGRVDQPKKLE
                     VTIPPGVDTGTRIRLAGKGEAGPFGSPPGDLYIFLHIKRHSVFDRDGTSLITRVPISF
                     TTAALGGEIEIPGLDGQRIGIEIPAGIQSGKQLRKRGCGMPVLQGRGRGDLVIEIMVE
                     TPTKLSARQKELLRELQATETGEETPQSKGFFDRLKEAWDDLTD"
BASE COUNT         1341 a         2379 c         2172 g         1212 t
ORIGIN      
        1 cgcggtcagc gccgcaacga tcttgatggt tgccagggtg tcgggcacca caaccccttc
       61 gggcgatgcg gcatgggcgg atgccagcgc ccattcgaac gaccagaaca ccgggaagag
      121 gaacagcagc gtcgccacat agccccagac gatcggcggt ttgcggccga cccgatcgga
      181 caaggcgccc gccccgacaa agaacaccaa cccgaacagc gcggaacctg caacgatcag
      241 ctgggccacg ccttcttgca cccgcatgtg cttgacgagg aaatcgaggc tcgaaaaggt
      301 cgcggtatac cagatcacgg tcagcccgac cgccaccccg aacagtgcca cgaaaatacg
      361 cttcttgttg ccgggatagg tgaagctttc gacgaagggg ttgccggcga tctggccttc
      421 ttccttcatc gcccggtaaa ccgggctttc cgacagcttg acccgcatcc acagcgagat
      481 cgccagcagc aggatcgaaa gcaggaacgg cacgcgccag ccccattcgg cccaggtcgc
      541 atcatcgaac atcgccttgc agccaagcac caccgccagg ctcagcacga aaccgccaac
      601 cacgctggcc tggatgaagc cggtatagaa gccgcgcttg ttgggcgggc tgtgctccga
      661 cacatagatc gctgccccgc cgtactcccc gcccaaggcc aggccttgca ggacgcgcag
      721 gaagataatg atggccggcg cccaaaggcc gattgattcg accgaaggaa ccaacccgac
      781 cccggcggtt gccacgccca tcagggtaac cgtgaccagg aaggtatatt tgcggcccag
      841 tttgtcgccc agatagccga acaggatcgc gccaagcggg cgaaagccga accctaccgc
      901 gaagcccgcc caggttacca gttcgcggac cagggcattg tcggaaggaa caaaggcctt
      961 gccgatgatc gcggccagcg tgccgtaaat gaagaaatcg taccattcga acacggtgcc
     1021 ggcggatgat gcgccgatca ccagcctgat gtccgatgca cttggctgaa cgtccggatg
     1081 acgggccggt tcagtcattt caaggctctc cccgttgatt ggctttgcgc ctgctctagc
     1141 gggggaatca gcttgtggga agcgcctcga tcccggcttt ccaggccagg gttatggcac
     1201 catgccgggc cggaaaatcg cgggccgcgg cgatcgctgc caggccgggc caagccggca
     1261 aatcgctctt accggccagc caggcttcga tcgccccgca ggcctggcca agctcggtcc
     1321 gggttcttcc gatggcagcc tggcaaaaga tcgcggccgc ggcctgcccg attgcacagg
     1381 cgtgcgcccg caacccgacc ctgcaaatcc gcccctcgct gtcgagatcg agccccaggt
     1441 caatgctcga cccgcaggtt tgggaacgcg actggccacg aaaccgcagg ccgtcatcga
     1501 gaggatagcg ggcaagcgcc gttgccagcc cgagaacgtc gggggtgtag agccttgcca
     1561 gggaggacaa agtcagtcct tctttttctt caggcgttcg gattcggccg cagcagcggc
     1621 gtgccgccgt tcggcgatca tgctgacaag ctgttcgctc gatgcattga tgaacggata
     1681 ggaacgggcc tgggtaatcc aggtgggccg accgccgaca ccccagaccg tatcataacc
     1741 aagcacgaac accgaaaaag tcatgactgc aatcacgaca cccttgaccc caccgaagcc
     1801 gaaacccagc acccggtcga tcgggccaag aaccgaattg cggctcgcct cgccaagccg
     1861 gttcgacagc agcttgacga tcgcataggg caccagcagc agcaaggcga aagccagtac
     1921 cgccgcaccg ctttccgtcc cgacataggc aaccagcgct gccgacagcg gtgtgtgcat
     1981 gtaatggatc gtcaccagcg ccagaaccca cgccgcaagg gccatgattt cctgaacaaa
     2041 accccgggca aacccggtga tggcgccaag gccgacgatc agcaggacag caatgtcaaa
     2101 tccggtcatg aagttaaatt aggctccgcc catgatccgg tcaacgagat ttggcagcat
     2161 ggaaagtgtt gaaactttta tctctttttc atgtcgtccg ctgtcaccag gcccgaaggc
     2221 ctgactgaaa ccaagcttgg ccgcttcgcg caggcggatc gggccatgtg ccaccgggcg
     2281 gatttcacca gccagcgaaa cctcgccgaa ccagatcgtg tcggcgctca gcggtttgtc
     2341 cgccagtgcg gagaccagcg ccgccgctac cgcaagatcg gcggccggat cggtcagccg
     2401 atagccgcct gcgacattca ggtagacttc ggccgaggag aaattcagcc cgcaccgcga
     2461 ctcaagcacg gcaagcagca tcgccaaccg cccgctgtcc cagcccacca ccgcacggcg
     2521 gggtgtggcg ccgctggcaa ggcgcacgat cagcgcctgg atttcgacca gcacgggccg
     2581 ggttccctcg atcgccggga acaccgaact gccggccacc ggctggtccc gccccgaaag
     2641 gaacagcatc gagggattgc ccacttcggc cagcccctgc ccttccatcg cgaaaacgcc
     2701 aatctcgtca accggtccga agcggttctt gatgcagcgt agcacccggt actggtggct
     2761 gcgctcgccc tcgaagctca tcaccacatc gaccatgtgt tcgagcaccc gcggcccggc
     2821 aatagtgcca tccttggtga catggccgac cagcaccagc gcaacgccgc tttccttggc
     2881 atagcggatc agttcgaagg ccgaggcgcg gacctgactg accgtgcctg gcgcgccttc
     2941 gatctggtct gaatgcatgg tctggatcga atcgataacc agcagggctg gcgccttcat
     3001 ctgccccagc gtggtcagga tatcccgcac cgaggttgcg gcggccagcc ggatcggcgc
     3061 accggcaaga ccaagccgtt ccgccctgag gcgcacttgc ccggccgcct cctcgccgct
     3121 gatgtagacc gcatcgccgc cgcctgcagc gatctttgcc gcggcctgaa gcagcagggt
     3181 ggacttgccg atccctggat caccacccat cagcacggcc gagccaggaa ccagccctcc
     3241 gcccaatgcc cgatcgaatt cggcaagccc ggtggaacgg cggcgcagtt tctcgccagg
     3301 tgcgtcgagc gattcgaagg caatcgcccg ccccccgctc gacaggtcgt ggcgcgcgga
     3361 aaaaaccgtc tccggcgcct cttcggcaag cgtgttccac tccccgcaat cggcgcattg
     3421 gccctgccaa cgctgggcga tcgacccgca ggcctggcaa acatatcggc gcttcggctt
     3481 ggccatgggc aaatccctaa ccggaacgta atgagaacgc aattgccttc gcgttacggt
     3541 cgtggaatga ggtgaccatg cgccaaaagg aacttcgcat cgcactggtc tgctacggcg
     3601 gcgtaagcct ggcagtatac atgcacggcg tgaccaagga attgtggaaa ctggcccggg
     3661 ctagccgcgc tttccttgaa ggagaacggc ccgagcacgg taccgaggcg gtctatgcgc
     3721 agctgctgca gcggatcgag gccttgttca acctgcgcct gcgcgtcctg cccgatatcg
     3781 tcgccggagc cagcgcgggc ggcatcaacg ggatcttcct ggcccaggca atccattccg
     3841 ggcagagcct cgagccgctg accgcgctgt ggctggaacg cgcggatgtc gacgtgctgc
     3901 tcgatcccga tgcccgtccc tggtcgcggt ttgcgaagtt ctgggcgatg ccgctggtgt
     3961 ggttcatgct caagcggccg ggcaacatgg tgtcggccag tgtcgcaccc gaaaccaggg
     4021 cagaggtgcg agccaagatt tcgcggctta tccgttcgcg ctggttcgag gcccctttcg
     4081 gcgggctggg cttttcccgg ctgctgaccg aggcgctcga cgccatggcg agcggcggat
     4141 cagaaaagcc gctgctgcct cccggccacc cgctcgacct gttcgtgacg gccaccgatt
     4201 ttcgcgggca cgaggaaatt gtcaggctgc acagccccga aaagatcgcc gagagcgagc
     4261 atcgcctgtc gatcggcttc agggccctga caccgagcga gcctggaaac aggctggcgc
     4321 ccctgcccga actggtcttc gcggcgcgcg gaacggccag ctttcccggg gcttttccgg
     4381 cactgcaact ggccgaaatc gataccctga tgcacgaacg cggcgaacac tgggcaagcc
     4441 gcaccgcgtt cgtcgagcgg atcatgcccg agcattcgca tcgcggcgat gccgaaaaag
     4501 tggcgctgat cgatggttcg gtgctggtca atgcgccatt tgtcgaggcg atgagcgtgc
     4561 tgagcgatcg gcccgcccag cgcgagatcg atcgccggtt cgtctacatc gacccgaagc
     4621 ccgacgagat tggcggacag cgccattccg atccgcgtgc tcccgggttc ttctcggtca
     4681 ttttcggttc gctgtcctcg atcccgcgcg aacagcctat ccgcgataac cttgaagtca
     4741 ttgcccagcg ctcgcggcgg ctcagtaccc tgaaggggat catcgattcg ctgcgtgtag
     4801 aggttgagca gacggttgaa gaggtattcg ggcggaccct gtttctcaac cgaccgacgg
     4861 ttgaccgcct ggaggcctgg cgctccaaag cacagcaaac tgcagccgaa cgcgccggct
     4921 atgccttcga ggcctatggc caggtcaagt atcggggcat cctgcaggat ctggccggca
     4981 cgatcctcgc cgccgcaccg gaactgatgc tgcctagcgc cgatccaatc gccgatcacc
     5041 ttgccgctta cctcgaacag cacgggatcg accggattgc cggccgaaag ggcggcgcca
     5101 gtcccgaggc gatcgcgttc ctgcgcgacc acgacatttc cttcaggatt cggcgcttgc
     5161 gcctgctggc gcggcgattg tcggaagatt ggcaggatct cgatgccgat cttggcgccg
     5221 cccgcgaaaa ggcgcgcgat gcggtctacc gtgcgctctc gctttatttc gaccggcagg
     5281 cgcgcagcgc gctggggagc gatttcggcg caatcgcaag gctggtcctg gaccggccgg
     5341 gtcacgtgct cgataccatc gcccagcgcc gccagctgcc ggcaaccgat ctggtggtcg
     5401 atgccatgct ggccgaagcc atggccgaaa tgccgcgcgc tttgcgccgc aaggtcttgc
     5461 tgacctatct gggtttcccg ttctacgaca cggtcacgct gcagttgctg cgcggcgaag
     5521 gttcaagcga actggaacca gccaaggtcg accgcatctc gcccgaagac tgcaattcga
     5581 tccgcgatgg cggggccgaa accacgctaa ggggcaccga gttctacaat ttcggagctt
     5641 tcttcagccg cgcctatcgc gagaacgact atctctgggg ccggctgcac ggcgtggaac
     5701 gcatgatcga cctcttggtt tcggcactgc cgcccggctt cgaactggat gcgcaagagc
     5761 tcgcccagtt caagcgccgc ggcttcgaaa ccgttcttga cgaagaacag gaccggctcg
     5821 tgacagatcc gaacctggtg ccctcgatca gggcggaact gcaccgcctc gaaaactagt
     5881 cggtcaagtc gtcccaggct tccttgagcc ggtcgaaaaa gcccttggac tgcggggttt
     5941 cctcgccggt ttcggtggcc tgcagttcgc gcagcagttc cttttgccgg gccgaaagct
     6001 tggtcggagt ttcgaccatg atctcgatta ccagatcacc ccggccccgg ccctgcagca
     6061 ccggcatgcc gcagccgcgc ttgcgcagct gcttgcccga ctggattccg gccgggatct
     6121 cgatcccgat ccgctgcccg tcgaggcccg gaatctcgat ctccccgcca agcgcagcgg
     6181 tggtaaagct gatcggcacg cgggtgatca agctggtgcc atcgcgatcg aacaccgagt
     6241 gccgcttgat gtgcagaaag atgtagagat cgcccggcgg tgatccgaag ggcccggcct
     6301 cacccttgcc ggcaaggcgg atccgggtgc cggtatcgac gcccggcggg atggtaactt
     6361 caagcttctt gggctgatcg acccggcctt cgccgcggca gctacggcac ggcttttcga
     6421 tcacctcgcc gcggccatgg caggtcgggc aggtccgctc gaccatgaag aagccctgct
     6481 gggcgcgcac ctggccgtgc ccgccgcaca ggttgcagcg ccgcgtcccg gtgcccggct
     6541 cggccccgct gccatcgcac ggctcgcatt tgctggccac ttcgatctcg atctcggcct
     6601 gcttgccatg aaaggcatca tcgagcccga tttcgaggtc atagcgcatg tcggcgccgc
     6661 gccgcgcctg ctggcgcccg ccgccgccaa aggcggaacc gaagatcgtc tcgaaaatgt
     6721 cgcccagatc ggaaaagccg ccgcccatgc ctgcgccgcc gccaccgccg cccgacatgc
     6781 cctgcttgaa ggcttcgtgg ccgaaccggt cgtaggcggc gcgcttttgc ggatccttca
     6841 ggcaatcata ggcttcgttg atcgccttga agcggccctc ggcctcggca tcgccggggt
     6901 tcttgtcggg atgccagcgc attgccagct tgcggaacga ggacttgatc gtcgcatcgt
     6961 ccgcgccgcg ttcaacttca agcagttcgt aatagtcggc tactacagcc attgccccac
     7021 cccgatgcag gacccgccgc cgcgctgcaa ggcacggcgg cgggcatcat gcatggcctg
     7081 atcagccctt gttgctgtcg ttga
//
LOCUS       DASUDV010000583         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1169395,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000583 DASUDV010000000
VERSION     DASUDV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169395"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1072)
                     /locus_tag="VFV30_12915"
     CDS             complement(5..1072)
                     /locus_tag="VFV30_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX4849039.1"
                     /translation="MSDSLFTPVALGDIQLSNRIVMAPMTRDRAGPGDVPTDTMVEYY
                     VQRASAGLIVTEGVQPSPAGKGYWRTPGIHSADQIEGWRRVAAAVHARGGRIVMQLMH
                     CGRVVVPANRGFEADVLAPSAIPCPVQVPGPDGAPMDCATPRAIAAADIPALLAEYAA
                     AARNAVAAGLDGVELHCASGYLINQVLNPASNHRTDEFGGSPENRARFPLMVLKALAE
                     AIGPGRVGFRISPGNPYNGMAPADPAATFGPFVQGADALGLAYLHVVDMGLPDLDTLG
                     FLRTNFSGPIVTNNNLKADSARAVLDAGKAEAVSFGRAFIANPDLVERIAAGAPLAKP
                     DYALLYTGEERGYTDYPALSA"
     gene            1158..1910
                     /locus_tag="VFV30_12920"
     CDS             1158..1910
                     /locus_tag="VFV30_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4849040.1"
                     /translation="MNYGFDGKVALVTGASGGIGRATALGFAASGAAVVVSDVNDAGG
                     EETVALIEAAGGQAVYQRCNVADAGEVKALVAKAVSAFGRLDFAHNNAGINNMGSDEY
                     DDAVWDRAIAINLSGVMYCMREEAEVMLKQGKGAIVNTSSINGLVGNPAQPGYTATKH
                     GVIGLTRHGALRWAKAGIRVNAVCPGVIETPMTAPLAANPDMRKILDGLTPMGRMGQA
                     EEIAAAVLWLCSDQASFVTGHPLVVDGGATAT"
     gene            complement(1995..>2293)
                     /locus_tag="VFV30_12925"
     CDS             complement(1995..>2293)
                     /locus_tag="VFV30_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4849041.1"
                     /translation="YGLLVRVDNAEPTADDIALARAYEEAGVDAFAQDFEIWDNKRPC
                     LNPMMVRGDGPFDKVRIWYRQFYNPRAQAETFQKRVNGRYTTKGTKTAPWESVA"
BASE COUNT          374 a          751 c          805 g          363 t
ORIGIN      
        1 ggcgtcaggc ggacagggcc gggtaatcgg tgtagccgcg ctcctcaccg gtatagagca
       61 gggcatagtc gggcttggcc agaggcgccc cggccgcgat ccgttcgacg aggtccggat
      121 tggcaatgaa ggcccggcca aagctgaccg cttccgcctt gcccgcatcc agcactgccc
      181 gggcgctgtc ggccttgaga ttgttgttgg tcacgatcgg gccgctgaaa ttcgtgcgca
      241 ggaatccaag cgtgtcgagg tccggcaggc ccatgtccac cacgtggaga taggccagac
      301 ccagcgcatc ggcgccctgc acaaagggcc cgaacgtcgc cgccggatcg gccggagcca
      361 tgccgttgta ggggtttccg ggagagatcc ggaagccgac ccggcccggc ccgatcgcct
      421 cggccagcgc cttcagcacc atcaacggaa agcgcgcgcg gttctctggc gagccgccga
      481 attcgtcggt gcggtggttg ctggcggggt tgaggacctg gttaatcagg taaccgctgg
      541 cgcagtgcag ctccacgccg tccagcccgg ccgccaccgc gttgcgggca gcggccgcgt
      601 attctgccag cagcgcggga atatccgccg cggcgattgc gcgcggcgtg gcgcaatcca
      661 tcggcgcgcc atcggggccc ggcacctgga ccgggcaggg aatggccgac ggcgcaagca
      721 catcggcctc gaacccgcgg ttggcgggaa ccaccacccg cccacaatgc atcagctgca
      781 tcacgatccg cccgccgcgc gcgtggacgg ccgcggccac ccggcgccag ccttcgatct
      841 gatcggcgct gtggatgccc ggggtgcgcc aatagccctt gccggccggg ctgggctgga
      901 caccctcggt cacgatcagt ccggcgcttg cccgctggac atagtactct accatcgtgt
      961 cggtcggcac gtcgccgggt cccgcccggt cgcgagtcat cggggccatg acgatacggt
     1021 tggagagctg gatatcgcca agcgccacgg gcgtgaaaag gctgtcagac attcgtgttc
     1081 aactcccgca atcggcgatt gaccgctatt tgcgttatcc tgtaagccat tttttacgca
     1141 tatcaaggag aggcaggatg aattacgggt ttgacggcaa ggtcgcgctg gtgaccgggg
     1201 ccagcggcgg gatcggccgc gccaccgcgc tgggctttgc cgcctcgggc gccgcggtgg
     1261 tcgtgtcgga cgtcaacgat gccggcgggg aagagaccgt ggccctgatc gaagcggcgg
     1321 gcggccaggc cgtctaccag cgctgcaacg tggccgacgc gggcgaggtc aaggcgctgg
     1381 tcgccaaagc cgtcagcgcg ttcgggcggc tcgactttgc ccacaacaac gccggcatca
     1441 acaacatggg atcggacgag tacgacgatg ccgtgtggga ccgggcgatc gcgatcaacc
     1501 tgtcgggcgt gatgtattgc atgcgcgaag aggccgaggt gatgctgaag cagggcaagg
     1561 gcgcgatcgt gaacacctcg tcgatcaacg ggctggtcgg caatccggcc cagccgggct
     1621 acaccgccac caagcacggc gtgatcggcc tgacccgcca cggcgcgctg cgctgggcca
     1681 aggccgggat ccgcgtcaac gcggtttgcc ccggggtgat cgaaaccccg atgaccgcgc
     1741 cgctggccgc caatccggac atgcgcaaga tcctggacgg gctgacccca atggggcgga
     1801 tgggccaggc cgaggaaatc gccgccgccg tgctgtggct gtgttccgat caggccagtt
     1861 tcgtgaccgg ccacccgctg gtggtggacg gcggcgccac ggcgacctga gcgcccccta
     1921 tcgccagaca aaaagagggc ccgctccggt caaccggggc gggcccttgc agtagagagg
     1981 cttgtgtcgg ctggtcaggc cacgctttcc cacggggccg tcttggtccc cttggtggtg
     2041 tagcggccgt tgacgcgctt ctggaacgtt tcggcctgcg cgcgcgggtt gtagaactgg
     2101 cggtaccaga tccggacctt gtcgaacggc ccatcgccgc ggaccatcat cgggttgaga
     2161 caggggcgct tgttgtccca gatctcgaag tcctgggcga aggcatcgac cccggcttcc
     2221 tcataggcgc gggccagggc gatatcgtcg gcggtcggct cggcgttgtc gacccggacc
     2281 agcaggccgt acc
//
LOCUS       DASUDV010000584         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1699768,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000584 DASUDV010000000
VERSION     DASUDV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699768"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..459)
                     /locus_tag="VFV30_12930"
     CDS             complement(115..459)
                     /locus_tag="VFV30_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEX4849042.1"
                     /translation="MKATIWHNPACGTSRKTLAILEEAPGVELTVVEYLANPPSADKL
                     AQLYRDAGISPQQGLRLRGTDAEERGLPGASDATVLAAMAAEPRLIERPLVETEKGVR
                     LCRPQDKVREIL"
     gene            594..2687
                     /locus_tag="VFV30_12935"
     CDS             594..2687
                     /locus_tag="VFV30_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX4849043.1"
                     /translation="MSTSLRLSLAFSAALAPLAWASPALGATEAAIEGEAPAIIVYGR
                     PQGYDIEKTPTATKTQTPLVDVPQAVSVISREQLDDQASHSLGDALRFVPGVVLGQGE
                     GHRDQITLRGQNSTADFFVDGLRDDAQYYRPLYNAERVEVLKGANALIFGRGGGGGVV
                     NRVSKAPEFDRTRIGSDASVDSFGAWSLATDIGAPLSDQVGTRLNTIYEEFDNHRQHY
                     AGHFLGITPTIGAHLGEATDLSLTYEFVDDHRTTDRGVPSRAGSPLRGYDETFFGSPT
                     VNRSDVTAHLAHARLDHEFSDSLSANLTAQFGHYDKYYGNVYPRAAVTTANTVALEGY
                     RSNTVRENWIAQGNLVWKGETGDLGHTLLLGFEAMDQDTRATRAEAVLSAATVALAER
                     ITAPAVSFGAKSRSSHSDVRALSFYLQDQVDIGDHIKLVAGLRYDDLTIEATNLLAAP
                     SPTTARRTDRKWSPRLGLILKPSENISVYGSYAISFLPQSGDQFTTLDATQATLAPEK
                     FENLEAGVKWDLTSALAFTLAAYQLERSNSRFNDPTTGTALLSGKTRAQGIEATLVGQ
                     LARNVQVSLGYALQEGEVRSATTAAPAGRKLAQLPRHQASAWVRYNLTSKLGLGLGVL
                     HQSSQFTTISNQVRLPAFTRIDAAVFYDLSDRVSLQVNVENLTDTDYFPNAHTDNNIS
                     VGEPFNARATIKVKF"
     gene            complement(2688..3224)
                     /locus_tag="VFV30_12940"
     CDS             complement(2688..3224)
                     /locus_tag="VFV30_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4849044.1"
                     /translation="MPRLTVNGRPVEFSVDAQTPLLWALRDAANLTGTKYGCGVGDCG
                     ACMVLVDGEALKSCLISLAEAEGRVVTTIEGLSPDRSHPVQQALVAEQAIQCGFCTPG
                     IAIAAAALLARNADPTPEDIFAAVPNLCRCGVYPRLVRAIQRAGRVMRRLETISAAPA
                     PGIDPADAARAVPAMKGV"
BASE COUNT          601 a         1151 c         1005 g          568 t
ORIGIN      
        1 ctatggccac agctcgtggc tgggcttcgc cgcgatcgaa ctgctttgcg ccggggtgat
       61 cgtgctgggc ctcaagggca tggccagcga cgacgaattg ctgcggccca aatcctagag
      121 aatttcccgt accttgtcct gcggccggca cagccgcacg cccttttcgg tttccaccag
      181 cgggcgttca atcaggcggg gttcggcggc catagccgcc aggaccgtgg catcgctggc
      241 accaggcagg ccgcgctctt cggcgtcggt cccgcgcagc cgcaggccct gctgcgggct
      301 gatcccggca tcgcgataga gctgcgccag cttgtcggcg ctgggcgggt tggcgagata
      361 ttcgaccacg gtgagctcca ccccgggggc ttcctcgagg atcgccaggg tcttgcgcga
      421 tgtgccgcag gcgggattgt gccagatcgt ggctttcatt ggctttgctc cgtcagatcg
      481 tgaaccacgc cctaggcaaa aattcgcggc acgacaaatt tgatccttgt caatgcgagc
      541 gattcgcatt agcggcaatc tcgttattgc aaatcattct cattaaggtg ctcatgtcca
      601 cttcgcttcg cctctccctc gccttctccg ccgcacttgc cccgcttgcc tgggcgagtc
      661 cggcgcttgg ggcaaccgaa gcggcaatcg aaggcgaagc cccggcgatc atcgtttatg
      721 gccggccgca gggctacgat atcgaaaaga ccccgaccgc caccaagacc cagaccccgc
      781 tggtcgatgt gccccaggcg gtcagcgtga tcagccgcga acagcttgac gatcaggcca
      841 gccattcgct tggcgacgcg ctgcgcttcg tgccaggagt ggtgctgggc cagggcgaag
      901 gccaccgcga tcagatcacc ctgcgcggcc aaaattcgac cgccgatttc tttgtcgacg
      961 gactgcgcga cgatgcgcag tactatcgcc cgctctacaa tgcagaacgc gtcgaagtgc
     1021 tcaagggcgc caatgcgctg atctttggcc ggggcggcgg cggcggggtg gtcaaccgcg
     1081 tatcgaaggc tcccgaattc gatcgcaccc gaatcggcag cgatgccagt gtcgacagct
     1141 ttggcgcctg gtcgcttgct accgatatcg gcgcacccct gtccgaccag gtcggaacgc
     1201 ggctcaacac gatttacgaa gagttcgaca atcatcgcca gcactatgcc ggccacttcc
     1261 tggggatcac acccaccatc ggcgcgcatc tgggcgaagc gactgatctc agccttacct
     1321 acgaatttgt cgatgatcac cgcaccactg atcgcggcgt gccttcgcgg gccggctcgc
     1381 cgctcagggg ctatgatgaa accttcttcg gctcgcccac cgtcaaccgc agcgatgtga
     1441 ccgcgcacct tgcccatgcc cggctcgacc acgaattttc cgacagcctc agcgccaacc
     1501 tgaccgcgca attcggccat tacgacaagt actatggcaa cgtatatccg cgcgccgcgg
     1561 tgaccaccgc gaacaccgtt gcgctggaag gctatcgcag caacaccgtg cgcgaaaact
     1621 ggatcgcgca gggcaacctg gtctggaagg gcgagaccgg tgaccttggc cacaccctgc
     1681 tgcttggctt cgaagccatg gaccaggata cccgggcgac ccgcgccgag gctgtactgt
     1741 cggccgccac ggttgcgctg gccgaacgga tcaccgcacc cgccgtgagc ttcggcgcca
     1801 aaagccgctc gagccattcc gatgttcgtg cgctgtcgtt ctacctgcag gatcaggtcg
     1861 atatcggcga tcacatcaag ctggtcgcgg gtctgcgcta tgacgacctc acgatcgagg
     1921 cgaccaacct gctcgcggcg ccgagcccga ccaccgcgcg gcgcaccgat cgcaagtggt
     1981 cgccgcggct tggcctgatc ctcaagccca gcgagaacat ctcggtctat ggcagctacg
     2041 cgatcagctt cctgccgcaa tcgggcgatc agttcaccac gctcgacgcg acgcaggcga
     2101 cgctcgctcc cgaaaagttc gaaaatctcg aagccggggt gaagtgggat ctgaccagcg
     2161 cactggcctt cacgctggcg gcctaccagc tggaacgcag caacagccgc ttcaacgatc
     2221 cgacaaccgg caccgcgctg ctttcgggca agacccgcgc ccaggggatc gaagcgacgc
     2281 tggtcggcca actggcccgc aacgtccagg tttcgcttgg ctatgccttg caggaaggtg
     2341 aagtgcgttc ggccaccacc gccgcgcccg ccggccgcaa gctggcccag ctgccgcgcc
     2401 accaggcatc cgcctgggtc cgctacaacc taaccagcaa gctgggcctg ggccttggcg
     2461 tgctgcacca gtcgagccag ttcacgacga tcagcaacca ggtgcggctg cccgccttca
     2521 cccggatcga tgcggccgtg ttctatgatt tgtcagacag ggtcagcctg caggtcaatg
     2581 tcgagaacct gaccgacacc gactatttcc ccaatgccca caccgacaac aacatctcgg
     2641 tgggcgagcc cttcaacgcc cgggcaacga tcaaggtcaa gttctgatca gacgcccttc
     2701 attgccggaa ccgcccgcgc cgcatcggcc gggtcgatcc ccggtgcggg cgctgcgctg
     2761 atcgtttcca gccgccgcat cacccggccg gcccgctgga tcgcccgaac cagccggggg
     2821 tagaccccgc agcggcacag gttgggtact gcggcaaaaa tatcctccgg cgtggggtcg
     2881 gcgttgcgag ccagcagggc cgctgcggca atcgcgatac caggagtgca gaacccgcac
     2941 tggatcgcct gctcggccac cagcgcctgc tgcaccgggt gcgaacgatc aggcgaaagg
     3001 ccttcgatcg tcgtcaccac ccgcccttcg gcttcggcca ggctgatcag gcatgatttc
     3061 aacgcctcgc catcgaccag caccatgcag gccccgcaat cgcccacccc gcaaccatat
     3121 ttggtaccgg tcaggttggc ggcatcgcgc agcgcccaca gcagcggggt ctgcgcatcg
     3181 accgagaatt cgaccggtcg gccgttgacg gtcaggcgag gcatcggcca gccgcgctca
     3241 atcgcgccag ctgcggtcga tccggtcgat ccggcgcagc caggcgtcga aatcggccgc
     3301 gcccggcagg gtgcccggca cctgc
//
LOCUS       DASUDV010000585         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1611110,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000585 DASUDV010000000
VERSION     DASUDV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611110"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1426
                     /locus_tag="VFV30_12945"
     CDS             17..1426
                     /locus_tag="VFV30_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX4849045.1"
                     /translation="MSDLTSHPWFFCGIGGSGMLPLALILRGAGADVAGSDRSRDQGR
                     TPEKFAWLESLGFTLHPQDGSGIVSAQQTLVASAAVEDTVPEMVRAKELGCPRMSRAE
                     LLATLFNAAAEGIAIGGTSGKSTVTGMAGWILDRAGRDPTIMNGAVMKNYATPDAPFA
                     SARVGHGGVFVSEVDESDGSIALYRPTVAVLGNVSLDHKSMEELRDLFGAFLARAEVA
                     AVNFDDAEAAALAPRAQCLVSYGIDSAEAMIGVAPGSVIEGPVSLTARIEDRRDGSAI
                     PLTLKVPGRHNLSNALAAIAAANAAGVPVAEAVHALGSFEGLARRFDIVGTSPGGVTV
                     IDDFGHNPDKVAATLKTLKAHPGRVIAFFQPHGYGPLRQMGHELAEVFARLLGPDDLT
                     ILCDPVYFGGTVDRSEGSERIVDLIRGHGGQAEHIPARDACAERIAEIARPGDRVVIM
                     GARDDTLTRFARDLLARLA"
     gene            1480..2127
                     /locus_tag="VFV30_12950"
     CDS             1480..2127
                     /locus_tag="VFV30_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4849046.1"
                     /translation="MATAASNDQTFFQAIGLWDRAVGAANYILDNWWVWPFLLFVVWL
                     GLKLRNQHKLLGELDERCAAAFADMDALLAERHSLIGNLVETVKGFAGQEHKVLKDVI
                     DARARATSTTGGARMDAEAAVGSSLANLWSITETYPDLASSGHFRELRSELTRMEDRI
                     TASRKFYNLSVEELNGVRRAFPYNLVDSLAKTSNHEKFSLGDARPALGEPVKVAF"
     gene            2145..3539
                     /locus_tag="VFV30_12955"
     CDS             2145..3539
                     /locus_tag="VFV30_12955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4849047.1"
                     /translation="MAATGYVTHAAENRKRLWQVLGLYVLAIQMIGGLVAMLVLMVFD
                     PAHMLLVDPAGYFLRYGIPMTLIGAGLFWWIYSGHAEAVKRKLTVTNPSRMDEPRFLR
                     IAEEQCIALGVRVPQFGIIEVPQPNALAVGEGPARGLIAVTRGLLDQLDDDELAAVIA
                     HQAAHIRNGNTEVLAANYALMRTAVLLQVNNGLRFEDWRQLIIPIVFPPLLLIMLLSG
                     MITKASLELARMARRAVKLSRFHICDGDAVRVTHRPDALHSALVKIGGRGAFAGSEAF
                     DDLLFDGRNDADGGTHPPVVERLQTIARLAGDLMQPGRMRQDTRPAALRAAAAPAAGG
                     FGRRFAPEQFRPVAPPQEKPRRKPRELDGAELLKLMLTDWKQYKAYVAACNEWYEWRE
                     SDNRDVLGLKPGMRIPLAAACAFMLVLYWPSDGNWQGFARKLSPTYFSEIMGMMDGTF
                     CSGPSYPDGTCRET"
BASE COUNT          572 a         1246 c         1252 g          591 t
ORIGIN      
        1 gcataccgga gagacaatga gcgacctcac ctctcacccc tggttcttct gcggcatcgg
       61 cggatcgggc atgctgccgc tggcgctgat cctgcgcggc gccggggccg acgtggccgg
      121 atcggaccgc agccgcgacc agggccgcac gccggaaaag ttcgcctggc tggaaagcct
      181 gggcttcacc cttcacccgc aggacggcag cgggatcgtc tcggcacagc agaccctggt
      241 cgcctcggcc gcggtggagg acactgtgcc ggaaatggtc cgggcgaagg aactgggctg
      301 cccgcggatg agccgggcgg agctgctggc gacgctgttc aacgccgcag cggaagggat
      361 cgccatcggc gggaccagcg gcaagagcac ggtgaccgga atggccggct ggatcctgga
      421 ccgcgccggc cgcgatccga cgatcatgaa cggcgcggtg atgaagaact atgccacgcc
      481 ggacgcgccc ttcgcttcgg cgcgggtggg ccacggcggg gtcttcgtgt ccgaagtcga
      541 tgaaagcgac ggctcgatcg cgctctaccg gccgaccgtg gcggtgctgg gcaatgtcag
      601 cctggaccac aagtcgatgg aggaactgcg cgacctgttc ggcgcgttcc tggcccgggc
      661 cgaagtggca gcggtcaatt tcgacgatgc cgaagcggcc gccctcgccc cgcgcgcgca
      721 gtgcctggtc agctatggca tcgacagcgc cgaggcgatg atcggggttg cccccggatc
      781 ggtaatcgaa ggcccggtca gcttgaccgc gcggatcgag gaccgccgcg acggcagcgc
      841 gatcccgctg accctgaagg tgcccggccg ccacaacctg tccaacgcgc tggccgcgat
      901 cgccgccgcc aacgccgcgg gcgtgccggt ggccgaagcg gtccacgcgc tcggctcgtt
      961 cgaagggctc gcccggcggt tcgacattgt cggcaccagc cccggcggcg tgaccgtgat
     1021 cgacgatttc ggccacaacc cggacaaggt cgccgccacg ctgaagacgc tgaaggccca
     1081 tcccggccgg gtgatcgcct tcttccagcc gcacggctac ggcccgctgc gccagatggg
     1141 ccacgaactg gccgaagtct tcgcccggct gctcgggccg gacgacctga cgatcctgtg
     1201 cgatccggtc tatttcggcg gcacggtgga ccgcagcgag ggcagcgagc ggatcgtcga
     1261 tctgatccgc ggccacggcg gccaggccga gcatattccg gcccgtgacg cctgcgcgga
     1321 gcgcattgcg gagatcgccc ggcccggcga ccgggtggtg atcatgggcg cgcgcgacga
     1381 tacgctgacc cgctttgccc gggatctgct ggcccggctg gcttaaccgc ttggcaaccc
     1441 tctcttgcta agtcccctgt atctgtgggg gattttgcga tggccactgc tgcttcgaac
     1501 gaccagacct tcttccaggc catcggcctg tgggaccgcg ccgtgggcgc cgccaactac
     1561 atcctcgaca actggtgggt ctggcccttc ctgctgttcg tggtgtggct gggcctgaag
     1621 ctgcgcaacc agcacaagct gctgggcgag ctggacgagc gctgcgccgc cgcttttgcc
     1681 gacatggacg cgctgttggc cgaacgccat tcgctgatcg gcaacctggt cgaaaccgtg
     1741 aagggctttg ccgggcagga gcacaaggtg ctgaaggacg tgatcgacgc ccgtgcccgc
     1801 gccacctcca ccaccggcgg cgcccgcatg gacgccgagg ccgcggtcgg ctcgtcgctc
     1861 gccaacctgt ggtcgatcac cgaaacctat cccgatctgg ccagctccgg ccacttccgc
     1921 gagctgcgca gcgagctgac ccggatggaa gaccggatca ccgccagccg caagttctac
     1981 aacctctcgg tcgaggaact gaacggcgtg cgccgcgcct tcccgtacaa cctggtcgat
     2041 tccctggcga agaccagcaa ccacgagaag ttcagcctgg gcgatgcccg cccggcgctt
     2101 ggcgagccgg tcaaagtcgc gttctaagcg gctgagactt cgccgtggcg gcgaccggat
     2161 acgtcaccca cgcggccgag aaccgcaagc gcctgtggca ggtgctgggg ctctatgtcc
     2221 tggccatcca gatgatcggc gggctggtgg ccatgctggt gctgatggtg ttcgatccgg
     2281 cgcacatgct gctggtcgat ccggccggtt atttcctgcg ctatggcatc ccgatgacgc
     2341 tgatcggcgc cggcctgttc tggtggattt acagcggcca cgccgaggcg gtgaagcgca
     2401 agctgaccgt caccaacccc tcgcgcatgg acgaaccccg gttcctgcgg attgccgagg
     2461 agcaatgcat tgccctgggc gtgcgggtgc cgcagttcgg gatcatcgaa gtgccccagc
     2521 ccaacgcgct ggccgtcggc gaaggccccg cgcgcgggct gatcgcggtg acccgcgggc
     2581 tgcttgatca gctggacgat gacgagctgg ccgcggtgat cgcccaccag gctgcccata
     2641 tccgcaacgg caacaccgag gtgctggccg ccaactatgc gctgatgcgc accgcggtgc
     2701 tgctgcaggt caacaacggg ctgcggtttg aggactggcg gcagctgatc atcccgatcg
     2761 tgtttccgcc gctgctgctg atcatgctgc tgtcggggat gatcaccaag gcctcgctgg
     2821 aactggcgcg gatggcgcgg cgcgcggtga agctttcgcg ctttcacatc tgcgatggcg
     2881 atgccgtgcg cgtcacgcac cgccccgatg cgctccattc cgcgctggtc aagatcggcg
     2941 ggcgcggcgc ctttgccggc agcgaggcct ttgacgacct gctgttcgac ggccgcaacg
     3001 atgccgatgg cggcacccac ccgccggtgg tggagcggct gcagaccatc gcccgcctgg
     3061 ccggagacct gatgcagccc ggccggatgc ggcaggacac ccgccccgcc gccctgcgcg
     3121 ccgccgcagc ccccgcggca ggcggttttg gccggcgctt tgcgcccgag cagtttcgcc
     3181 cggtcgcccc gccgcaggaa aagccccggc gcaagccgcg cgagctggac ggagccgagc
     3241 tcctcaagct gatgctgacc gactggaagc agtacaaggc ctatgtcgcc gcctgcaacg
     3301 agtggtacga atggcgcgag agcgacaacc gcgatgtcct gggcctgaag ccggggatgc
     3361 ggatcccgct ggccgccgcc tgcgctttca tgctggtgct ctactggccc agcgacggca
     3421 actggcaggg cttcgcgcgc aagctcagcc cgacctattt cagcgaaatc atggggatga
     3481 tggacggcac cttctgctcc ggccccagct accctgacgg cacttgccgg gagacgtaag
     3541 atgagcgtgg tgagcaatat cctcagcaat gtcggcagca gcctcgttcg ccggctggtc
     3601 gtggtctttg tgctgctgat cgtgatccgc gggatcggct ttctggtcgc gccgagcagc
     3661 c
//
LOCUS       DASUDV010000586         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_43231,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000586 DASUDV010000000
VERSION     DASUDV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43231"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1398)
                     /gene="ftsA"
                     /locus_tag="VFV30_12960"
     CDS             complement(118..1398)
                     /gene="ftsA"
                     /locus_tag="VFV30_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX4849048.1"
                     /translation="MPPPRIAKLFAAVNIGSFRVSAMIAGLTDAGEMVVLGSGHRAAQ
                     GIKRGYITDMAAATFAVRDAVERAEKMANTSVTSVWIGASGAGLASQVAQVEVDIGGR
                     RIEAEDIEHLLVAARDVIQPDGRMVLHAQPAHYTLDGADGVADPKGLHAERLGVDIHV
                     MLADGAPIRNITEAVQNAHLQVESVVASPIAAGHACLGVEERELGVALVEFGGEVTNV
                     SVYAGGMLLGLVTIPLGSGDVTDAIASAFGIRRFQAERLKCVHGSAIASPADHREMIP
                     VNAPGEEGSGPPARHADDKNRIPRAELVSVITTQLARWMDEVSKALKGLGFTGQRGQL
                     VVLTGGGAELAGIADYAQAALGRPVRIGKPQHVKGLAEAHATPGFSTLAGLVLYAAAD
                     PIDIRAVGPRHQTTIRAVGAGLAERLIRAVREYF"
     gene            complement(1398..2339)
                     /locus_tag="VFV30_12965"
     CDS             complement(1398..2339)
                     /locus_tag="VFV30_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX4849049.1"
                     /translation="MSQKIRRGGKGVRRAAAAQGKARQVRAAKVRTGSAMDAVMNFVP
                     LSEEQLHRVFLAMILGAGVALAWIVASLAGVPTMAQHQIEAIAHDAGFEVQKVNVRGT
                     NHLNELKVYQLALAENNRAMPFVDLDGMRQRLLELSWVEDARVSRQLPDTLVVDIVER
                     KPVAVLKKVDRLVLIDARGKELEAVSAAKAKGKLMISGPGAGRQVENLAELLAAAPAL
                     KPQVREAEWVGNRRWNLTFRTGQVLALPEGDQPSAKALMTFARLDGVNRLLGGKAVAF
                     DMRASDRIYFRVPGRADPVEQPPRPATSEAKPKEEAN"
     gene            complement(2336..>2847)
                     /locus_tag="VFV30_12970"
     CDS             complement(2336..>2847)
                     /locus_tag="VFV30_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX4849050.1"
                     /translation="VGVAIVTADGNYGNPISPDAKGPWQEFDRLLAEPYIRGRELTTA
                     VLGNRALMVTELKPKSGFYDFDAKYTEGMTEHVCPAQIPEEIEKACLDLALRCHQLLG
                     CKGCSRTDFRWDDERGLEGLFVLETNTQPGMTPLSLVPEQARHLGIEYGELVEMIIAE
                     ALNGKEGKA"
BASE COUNT          495 a          977 c          873 g          502 t
ORIGIN      
        1 ggcaaggcgg atacattaac agctagtttg ttacctattt gcacccttcg aatcggcgat
       61 caaagccaat ctggggcgtt gtccacaggc gccgccgcaa gtgtaccggc gaaacaccta
      121 gaaatactcg cgaaccgcgc ggatcagtcg ctcggcaagg ccagcgccga ccgcgcggat
      181 cgtggtctga tggcgcgggc caaccgcacg gatatcgatc ggatctgccg cggcataaag
      241 caccagtccg gccagggtag aaaagcctgg agttgcatgg gcttcggcca gacctttgac
      301 atgctgcggc ttgccgatcc gcaccggacg ccccagcgcc gcctgggcat agtcggcaat
      361 tcccgccaat tccgccccgc ccccggtcag caccaccaac tggccgcgct gcccggtgaa
      421 gcccaggcct ttcagcgcct ttgaaacttc atccatccac cgggcgagct gagtggtgat
      481 caccgaaacc agttcggcgc gcgggatgcg gttcttgtca tcggcgtggc gggctggcgg
      541 ccccgaccct tcttcgccag gcgcgttgac ggggatcatc tcgcgatgat cggccggact
      601 ggcgattgcc gaaccatgga cgcatttgag gcgctcagcc tggaagcggc gaatgccgaa
      661 ggcgctggcg atggcatcgg tgacgtcgcc cgatcccagc gggatcgtca ccagacccag
      721 cagcatgccc ccggcataga ccgaaacatt ggtaacctcg ccgccgaatt cgaccagcgc
      781 gacccccagt tcgcgttcct cgacgccaag gcaggcgtga ccggcggcaa tcggcgaggc
      841 gaccacggat tcgacctgga ggtgcgcgtt ctggaccgct tcggtaatgt tgcggatcgg
      901 cgcgccatcg gccagcatca cgtgaatatc gaccccaagc cgctcggcat gaagcccctt
      961 ggggtcggcc acgccatcgg cgccgtcgag cgtataatgc gccggctggg cgtgcagcac
     1021 catgcgcccg tcgggctgta tcacatcgcg tgcggcaacc agcaggtgct cgatatcctc
     1081 cgcctcgatc cgcctgccgc cgatatcgac ctcgacctgc gcgacctggc tggcaaggcc
     1141 tgcccccgaa gccccgatcc acaccgaagt gaccgaggta ttggccatct tctcggcgcg
     1201 ttcgactgca tcgcgcaccg caaaggtcgc ggccgccata tcggtgatat agccgcgctt
     1261 gatcccttgt gcagcgcggt gccccgatcc cagcacaacc atctcacccg catcggtcag
     1321 cccggcgatc atcgccgaga cccggaacga gccgatgttg actgctgcga acagcttggc
     1381 gatccgcggc ggcggcatca gttcgcctct tccttcggct ttgcctcgct tgtggcgggc
     1441 ctgggcggct gttcgaccgg atcggcacgg cctggaacgc ggaaatagat ccggtccgag
     1501 gcgcgcatgt cgaacgccac ggccttgccg ccaagcagcc ggttgacccc gtcaagccgg
     1561 gcgaaagtca tcagcgcctt ggccgaaggc tgatcgcctt cgggcagggc cagcacctgc
     1621 ccggtcctga aggtcaggtt ccagcggcga ttgccaaccc attcggcctc gcgcacctgc
     1681 ggcttgagcg cgggagcggc agccagcaac tcggccaggt tttcaacctg ccgccctgcc
     1741 ccggggcccg aaatcatcaa tttgcccttg gccttggccg cagacaccgc ttcgagttcc
     1801 ttgcccctcg catcaatcag caccagccga tcaaccttct tgagcacggc caccggcttg
     1861 cgctcgacga tgtcgaccac cagcgtatcg ggcagctggc gcgagacccg cgcatcttcg
     1921 acccacgaaa gctcaagcag ccgctgacgc attccatcga gatcgacgaa tggcatggcg
     1981 cggttgtttt cggccagcgc gagctgatag accttcagtt cgttgaggtg gttggtgccg
     2041 cgcacgttga ccttctgcac ctcgaacccg gcatcgtggg caattgcctc gatctggtgc
     2101 tgggccatgg tcggaacccc ggccaggctg gccacgatcc aggccagcgc aacccccgcg
     2161 cccaggatca tcgccaggaa cacgcggtgg agctgctctt cgctgagcgg gacaaagttc
     2221 atcaccgcgt ccatggccga tccggtgcga accttggcag cgcggacctg gcgtgccttg
     2281 ccctgggcag cggcagcccg gcgcacgccc ttgccgccgc gacggatctt ctggctcatg
     2341 ccttgccctc cttgccgttc agcgcttcgg caatgatcat ctcgaccagt tcgccatatt
     2401 cgatccccag gtggcgggcc tgttcgggaa ccaggctgag cggggtcatc cccggctggg
     2461 tgttggtttc aagcacgaac agtccttcaa ggccgcgctc gtcatcccag cggaagtcag
     2521 tgcgcgaaca gcccttgcag cccagcagct ggtggcagcg cagcgccagg tcgagacagg
     2581 ccttctcgat ctcctcggga atctgcgccg ggcagacatg ctcggtcatg ccctcggtgt
     2641 acttggcatc gaaatcgtag aagcccgact tgggcttcag ttcggtgacc atcagcgcgc
     2701 ggttgcccag cacggcggtg gtcagttcgc ggccgcggat atagggttcg gccagcaacc
     2761 ggtcgaattc ctgccacggc cccttggcat cagggctgat cggattgcca tagttgccat
     2821 cggcggtgac aatcgccaca cctaccg
//
LOCUS       DASUDV010000587         6548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1081449,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000587 DASUDV010000000
VERSION     DASUDV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081449"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFV30_12975"
     CDS             <1..1254
                     /locus_tag="VFV30_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849051.1"
                     /translation="TDHYMQILANQAPIGFEAEFVNQRNRTILYRGILLPFSSDDETI
                     DFIYGVINWKELADQATTDELLLEIDQALEAKAPAELRRDDPLTEWADGPADTGLDLP
                     ETESAEAGDDVFDLAGLAVPDASDEDSADDDWAEPAPAPVTRDHGRFALPAFSAEDDE
                     DEDAPAAPLDLRALARAQEGHAPLELDGFEAEPAFEAADAAPEAPLAIDLPPVDETDM
                     ALGDWLASARELAEAARGSEDRTRSALYAAIGRAYDFSLAAAEAPEEFAELVADSGLT
                     AQDRAPMTPVVKLVFGADYDKTRLAEYATALSHAQRIGLGRGSLGQYLAAAPGGLKGV
                     VSEERRIKRGETAPAAPARDTVRTGLAKKLRKLPHAPFEALDGEGAEFTLLVARRLPD
                     GEVVLLGEIADDIPLLEKAAKKLLG"
     gene            1447..2361
                     /locus_tag="VFV30_12980"
     CDS             1447..2361
                     /locus_tag="VFV30_12980"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4849052.1"
                     /translation="MGWIGIVLALLVLAGAALWHYAHATNAVGLLNWADRTLAGTDGT
                     AVAQSGVAFGPEPGQQLDVIVPNTPGPHPVLVFIHGGGWHSGMPGDYGFVGRQFARAG
                     YVVVLPGYRLGPAGRYPAMLEDSALALAWVQANIAAQGGDPQRVFVMGHSAGAYNAVM
                     ITLERQWLGRAGVGEGFIKGAVGLSGPYDFFPWTSDSARNAFGHVADPASTQPIRFAR
                     GDAPPMLLLTGDSDDTVKPRNSKALAAALSAAGRPTEPVILAGVDHAGTVVKLAAPFN
                     RDRRVLDPVLAFLAAHGGASGAVQPQPR"
     gene            2373..3737
                     /locus_tag="VFV30_12985"
     CDS             2373..3737
                     /locus_tag="VFV30_12985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4849053.1"
                     /translation="MRAIHRFLARCLAALLALVLSVQPALAQSILRDAETELLLRDMA
                     APLVTAAGLDPRNVDLVLVNHPSINAFVAGGQIIFLHSGLINEAGSALEVQGVIAHEL
                     GHITGGHIINDSGSRAATGISILSMILGGIAAAAGSGDAAMGVIMAGQQAALGKYLAF
                     NRGQEASADAAGAQYLSKAGLSGRGSIQFFKRLQNLEMRHGWTRTYDSQFYRTHPLTS
                     DRIATLEDTYRADPAWNAPSDPVLEARFQRAKAKLFGYLAEPKDTLRVYPETMTSIPA
                     RYARAYAWHKDARLDKALAEADALLALAPDDPFFLELKGQILLESGRPREALAPLRRA
                     TELSGFQPLIATTFGHALVATEDPAQLDEAERVLKAAVSRDRENPFAWYQLGVIYGMK
                     GDIPRAQLASAEQQIMQLNYLQALRSAETAQAGLPQGSADWIRAGDIAMQARAAIEQQ
                     KKRR"
     gene            3749..4501
                     /locus_tag="VFV30_12990"
     CDS             3749..4501
                     /locus_tag="VFV30_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX4849054.1"
                     /translation="MADSSSRSTLPLVLSGIALLLTGALAGWTYARTQGGTMDKTAVE
                     RTVREYILANPEILPEAMENLQKKQASQQLGAVGDKLEEPFPGAVLGNPNGTVTLVEF
                     TDYACGYCRKSVPDVDALIAENPDLRVVVRELPIIAPTSPDAARWALAAAEQGKFPAF
                     HKAMFAAGRTDAASIEAAARAAGLDLDRARKFVAQPQVQAELARNLEMARQLGIDGTP
                     SWVAGDQLLAGAVGKAELGKAVAAARRGTDNR"
     gene            4553..5713
                     /locus_tag="VFV30_12995"
     CDS             4553..5713
                     /locus_tag="VFV30_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4849055.1"
                     /translation="MAVLPFRPIPFFANKNRAFWRLQAAGWGGAMLLRTASGLANGQP
                     LSFLVLVLISTITGLSVTLILSVIYRQLMGRRPLVTWGVTSLVLPLAVSLYAFIDAWV
                     NGLYAGDTGVGFAQRFIGLFYLDATLLLAWSALYYMINFFLQIEEQNDQLLRLENQAT
                     SAQLAMLRYQLNPHFLFNTLNSISTLVLLKQTEPANAMLSRLSSFLRYTLVNEPTGRV
                     TVAQEVDTLKLYLEIERMRFEERLRTRFQIEPGTEAGLLPSLLLQPLVENAIKYAVSP
                     QESGAEITIAAQLVGPNLRITVSDTGPGLQSGGTNNRLTGVTFDGGEQVSTGVGLANI
                     RDRLAQAYGEEHRFETMEPPEGGFAVLIEIPFERREPTPPALPTDKRPALAG"
     gene            5724..6518
                     /locus_tag="VFV30_13000"
     CDS             5724..6518
                     /locus_tag="VFV30_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX4849056.1"
                     /translation="MTIRTIIVDDEKLAIQGLQLRLDKFPDVEVIDTCSNGREAIRKI
                     KTEKPDLVFLDIQMPGFDGFSVVKGIMEIEPPLIVFVTAYQEHAVRAFEANAVNYLMK
                     PVDEDKLADTMERVRLRIAEKQAAEEAEKLKTVLAEVAPDAMDAMPEEPEHAARYEKL
                     INIKDRGQIFRVDVDSIERIEAAGDYMCIYTGDNSLILRETMKDLERRLDPRVFQRVH
                     RSWIVNLNQVRQVKPHTNGECFLVLESGADVKVSRSYRDVVARFVH"
BASE COUNT         1081 a         2292 c         2165 g         1010 t
ORIGIN      
        1 accgatcact acatgcagat cctggccaac caggcgccga tcgggttcga ggccgaattc
       61 gtcaaccagc gcaaccggac gatcctgtac cgcgggatcc tgttgccgtt ctcgtccgac
      121 gacgagacga tcgacttcat ctatggggtg atcaactgga aggaactggc cgaccaggcg
      181 accaccgacg agctgctgct cgaaatcgac caggccctgg aagccaaggc tccggccgag
      241 ctgcgccgcg acgatccgct gaccgaatgg gctgatggcc cggccgacac cggcctggac
      301 ctgccggaaa ccgaaagtgc ggaagcgggc gacgacgtgt tcgatctggc cggcctggcc
      361 gttccggacg catccgacga ggactcggcc gacgacgatt gggccgaacc ggcccccgcc
      421 ccggtcaccc gcgatcacgg ccgctttgcc cttccggcct tctcggccga ggatgacgag
      481 gacgaggatg cgccggccgc gccgctggac ctccgcgccc tggcccgggc ccaggaaggg
      541 cacgctccgc tggaactcga tgggttcgag gccgaaccgg ccttcgaagc ggccgacgcc
      601 gcgcccgaag cgccgctggc gatcgatctg cccccggtgg acgaaaccga tatggcgctg
      661 ggcgattggc tcgcctcggc ccgggaactg gccgaagcgg cgcgcggatc cgaagaccgc
      721 acccgcagcg cgctctacgc cgcgattggc cgggcctatg acttctcgct cgccgcggcc
      781 gaagcgccgg aggaatttgc cgagctggtc gccgattcgg gcctgaccgc gcaggaccgc
      841 gcgcccatga ccccggtggt caagctggtg ttcggcgccg attacgacaa gacccgcctg
      901 gccgaatacg ccaccgcgct gagccatgcc cagcgcatcg ggctgggccg cggatcgctt
      961 ggccagtacc tggctgccgc tcccggcggg ctcaagggcg tggtcagcga agagcgccgg
     1021 atcaagcgcg gcgaaaccgc gccggccgct cccgcccgcg acacggttcg caccggcctc
     1081 gccaagaagc tgcgcaagct gccgcacgcc ccgttcgagg cgctggacgg cgaaggggcc
     1141 gaattcaccc tgctggtcgc ccgccgtctg cccgatggcg aagtggtcct gctcggcgaa
     1201 atcgcggacg atattccgct gctggaaaag gcggcgaaga aactgctcgg ctgattacca
     1261 agtcccagtc ctgggtcagc cagcacggcg cgccatgtcg gggggcatgg cgcgcctttg
     1321 cgtttggagc cgggccgggc tgatccggca cggccagtcc tgcccgttcg gacgcttccc
     1381 ccgccgccga aaaccggata ggcccccgcc atggcagcgg cggcaggcag gaaacggatt
     1441 tcgcggatgg gctggatcgg gattgtcctg gcgctgttgg tcttggccgg ggcggcgctg
     1501 tggcactatg cccatgccac caatgcggtc ggactgctca actgggcgga ccggaccctg
     1561 gccggcaccg atggcacggc cgtggcgcaa agcggggtcg ccttcgggcc cgaacccgga
     1621 cagcagctcg acgtgatcgt acccaacacg ccggggccgc acccggtgct ggtcttcatc
     1681 cacggcggcg gctggcattc cggcatgccg ggcgactacg gctttgtcgg ccggcaattc
     1741 gcccgggcgg gctatgtcgt ggttctgccc ggttaccggc tgggcccggc cggacgctat
     1801 cccgccatgc tggaagacag cgcgctggcg ctggcctggg tccaggccaa catcgcagcc
     1861 cagggcggcg atccgcagcg cgttttcgtg atgggccatt cggccggggc ctacaacgcg
     1921 gtgatgatca cgctggagcg ccagtggctg ggccgggccg gggtgggcga aggcttcatc
     1981 aagggcgcgg tcggcctgtc gggcccttac gatttctttc cctggacctc agattcggcg
     2041 cgcaacgcct ttggccacgt ggccgatccc gccagcaccc agccgatccg ctttgcgcgc
     2101 ggcgatgcgc cgccgatgct gctgctgacg ggtgacagcg acgacacggt aaagccccgc
     2161 aattccaagg ccctggccgc cgcgctcagc gcggccgggc gcccgaccga accggtcatc
     2221 ctggccgggg tcgaccatgc cgggacggtg gtgaaactgg ccgctccgtt caaccgcgac
     2281 cgccgcgtgc tcgacccggt cctggccttc ctggccgcgc acggcggggc ttcaggtgcg
     2341 gttcagccgc aaccccgcta ggagtgggtc caatgcgcgc cattcaccga tttctcgccc
     2401 gctgccttgc cgccttgctg gcgctggtgc tcagcgtcca gccggccctg gcgcagtcga
     2461 tcctgcgcga cgcggaaacc gaattgctgc tgcgcgacat ggccgcgccg ctggtcaccg
     2521 cggccgggct cgatccgcgc aacgtcgatc tggtgctggt caaccacccc tcgatcaacg
     2581 cctttgtcgc cggcgggcag atcatcttcc tccattccgg gctgatcaac gaggccggtt
     2641 cggcgctgga ggtccaggga gtgatcgccc acgaactggg ccacatcacc ggcggacaca
     2701 tcatcaacga ttccggctcg cgcgcggcta cgggcatctc gatcctgtcg atgatcctgg
     2761 gcggcattgc cgccgcagcc ggatcgggcg acgcggcgat gggcgtgatc atggccggcc
     2821 agcaggccgc gctgggcaag tacctggcct tcaaccgcgg gcaagaggcc agcgcggacg
     2881 ccgccggggc gcagtacctg tccaaggccg gcctttccgg ccgcggttcg atccagttct
     2941 tcaagcgcct ccagaacctg gaaatgcgcc acggctggac ccgcacctac gattcccagt
     3001 tctaccgcac ccacccgctg acctcggacc ggatcgccac gctggaagat acctaccggg
     3061 ccgatccggc ctggaacgcc ccgtccgatc cggtgctgga ggcgcggttc cagcgggcca
     3121 aggccaagct gttcggctat ctggccgaac cgaaggacac cctgcgggtc tatcccgaaa
     3181 cgatgaccag cattccggcg cgctatgccc gggcctatgc ctggcacaag gacgcgcggc
     3241 tcgacaaggc gctggccgag gccgatgccc tgctggcgct ggccccggac gatcccttct
     3301 tcctggagct gaagggccag atcctgctgg aatcgggccg cccgcgcgag gcgctggcgc
     3361 cgctgcgccg cgcgaccgag ctgagcgggt tccagccgct gatcgccacc acctttggcc
     3421 acgcgctggt cgcgaccgag gatccggccc agcttgacga ggccgagcgg gtgctgaaag
     3481 ccgccgtctc gcgcgaccgg gagaacccct ttgcctggta ccagctgggg gtgatctatg
     3541 gcatgaaggg cgatatcccg cgggcccagc tggccagcgc ggagcagcag atcatgcagc
     3601 tcaactacct tcaggcgctg cgcagcgcgg aaacggccca ggccggcctg ccgcaaggct
     3661 cggccgactg gatccgcgcc ggggatatcg cgatgcaggc gcgcgccgcg attgaacagc
     3721 agaagaaacg caggtagcca cctgccgcat ggccgatagc tcttcccgtt ccacccttcc
     3781 gctggtcctg tccggcattg ccctgctgct gaccggcgcc cttgccggct ggacctatgc
     3841 ccgcacccag ggcggcacga tggacaagac cgcggtggag cggaccgtgc gcgaatacat
     3901 cctcgccaat cccgaaatcc tgcccgaagc gatggagaac ctgcagaaga agcaggccag
     3961 ccagcagctg ggcgcggtgg gggacaagct ggaagagccg ttccccgggg ccgtgctggg
     4021 caatcccaac ggcacggtca ccctggtcga gtttaccgac tatgcctgcg gttattgccg
     4081 caagagcgtg cccgatgtcg acgcgctgat cgcggagaat ccggacctgc gggtggtggt
     4141 gcgcgaattg ccgatcatcg ccccgaccag cccggatgcc gcccgctggg ccctggccgc
     4201 ggccgagcag ggcaagttcc ccgccttcca caaggcgatg ttcgccgccg ggcggaccga
     4261 tgccgcctcg atcgaggccg cggcccgcgc cgccgggctc gatctggacc gggcccgcaa
     4321 gttcgtggcc cagccccagg tccaggccga actggcccgc aacctggaaa tggcccgcca
     4381 gctgggcatc gacggcaccc ccagctgggt ggcgggcgac cagttgctgg ccggcgcggt
     4441 cggcaaggcc gagctgggca aggccgtggc cgccgcgcgg cgcggcacgg acaaccgctg
     4501 atcctcacgg aatccctctt ggtgcccgcg aaaacctgct aaggactggc ccatggccgt
     4561 gctgcccttt cgcccgatcc cgttcttcgc caacaagaac cgcgccttct ggcggctgca
     4621 ggccgcgggc tggggcgggg ccatgctgct gcgcaccgcg tcgggcctgg ccaacggcca
     4681 gccgctgtcg ttcctggtcc tggtcctgat ctcgaccatc accggcctgt cggtgaccct
     4741 gatcctgtcg gtgatctacc gccagctgat gggccgccgc ccgctggtga cctggggggt
     4801 cacctcgctg gtcctgccgc tcgcggtctc gctctatgcc tttatcgatg cctgggtgaa
     4861 cgggctttac gccggcgaca ccggggtcgg ctttgcccag cgcttcatcg ggctgttcta
     4921 cctcgacgcg accctgctgc tggcctggtc ggcgctctat tacatgatca acttcttcct
     4981 tcagatcgaa gagcagaacg accagctgct gcggctggaa aaccaggcga ccagcgccca
     5041 gctggcgatg cttcgctacc agctcaaccc gcacttcctg ttcaacacct tgaattcgat
     5101 ctccaccctg gtgctgctga agcagaccga gccggccaac gccatgctca gccgcctgtc
     5161 cagtttcctg cgctacaccc tggtgaacga gccgaccggg cgggtgaccg tggcgcaaga
     5221 ggtcgacacc ctgaagctct acctcgagat cgaacggatg cgcttcgagg agcggctgcg
     5281 cacccggttc cagatcgaac cggggaccga ggccgggctg ctgccctcgc tgctgctcca
     5341 gccgctggtc gagaacgcga tcaagtatgc ggtcagtccg caggaatcgg gcgcggagat
     5401 caccatcgcg gcgcaactcg tcggcccaaa ccttcgcatc accgtctccg acaccggccc
     5461 ggggttgcaa tcggggggta cgaacaacag gttgaccggc gtgacattcg atggcggcga
     5521 acaggtttca accggggtcg gcctcgcgaa cattcgcgac cggctggccc aggcctatgg
     5581 cgaagagcat cgtttcgaaa cgatggagcc ccccgagggc ggctttgccg tgctgatcga
     5641 gatccccttc gaacggcgcg aaccgacgcc gcccgccctt cccaccgaca agcgaccggc
     5701 actggccggc taaggcccag cccatgacca tccgcaccat catcgtcgac gacgagaagc
     5761 tcgcgatcca gggcctgcaa ctgcgcctgg acaagtttcc cgacgtagaa gtgatcgaca
     5821 cctgctcgaa cgggcgcgag gcgatccgca agatcaagac cgagaagccc gacctggtct
     5881 tcctcgacat ccagatgccc ggctttgacg gcttctcggt ggtcaagggg atcatggaga
     5941 tcgaaccgcc gctgatcgtc ttcgtgaccg cctatcagga acacgccgtg cgcgcgttcg
     6001 aggccaacgc ggtcaattac ctgatgaagc cggtcgacga ggacaagctg gccgacacga
     6061 tggagcgggt acgcctgcgc atcgccgaga agcaggccgc agaagaggcc gaaaagctca
     6121 agaccgtgct ggccgaagtc gcccccgacg cgatggacgc catgcccgaa gagccggagc
     6181 acgcggcgcg ctatgaaaag ctcatcaaca tcaaggaccg cgggcagatc ttccgggtcg
     6241 atgtcgattc gatcgaacgg atcgaggcgg cgggcgacta catgtgcatc tacaccggcg
     6301 acaacagcct gatcctgcgc gaaacgatga aggacctcga acgccggctc gatccgcggg
     6361 tgttccagcg cgtccaccgc agctggatcg tcaacctcaa ccaggtccgc caggtcaagc
     6421 cgcacaccaa cggtgaatgc ttcctggtgc tggaaagcgg cgccgacgtg aaggtctcgc
     6481 gctcctaccg cgacgtcgtg gcgcggttcg tgcactgatt tgcggctgct gcgcacctgg
     6541 gctttcgt
//
LOCUS       DASUDV010000588         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1699915,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000588 DASUDV010000000
VERSION     DASUDV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699915"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFV30_13005"
     CDS             <1..400
                     /locus_tag="VFV30_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX4849057.1"
                     /translation="PGRLVLGSDSLVEVDGRRFDKPASREDAAAHLRFFSGKVMQLHS
                     AAALARDGGVIWRHGALARLRVRALSEQFITSYLGHEWPAVGACVGVFRIEARGVQLF
                     ESIEGDHFTVLGMPLLPVLGALRQHGNLLA"
     gene            397..1203
                     /gene="aroE"
                     /locus_tag="VFV30_13010"
     CDS             397..1203
                     /gene="aroE"
                     /locus_tag="VFV30_13010"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX4849058.1"
                     /translation="MTRPYAEVIGDPIAQSKSPAIHNFWLERLGIDAEYRACHVTTEG
                     LADYVAERRGDPQWRGCNVTMPHKQAVMALIDAIDPQAARVGAVNTVVSVAGQGLTGF
                     NTDVAGFLEPLGGRPFDCVSVIGAGGAARAILAALADRGTRWVSLQNRSIAKGEALLA
                     EFGLAGCVVEPGAEVPTAELLVNTSSLGMAGFPPLPAVEAFVMDGGTVYDIVTTPLET
                     DLIRRARARGLATIDGLAMLIGQAAAAFEKFFGQPAPRGHDAELRALLTR"
     gene            1200..1805
                     /gene="coaE"
                     /locus_tag="VFV30_13015"
     CDS             1200..1805
                     /gene="coaE"
                     /locus_tag="VFV30_13015"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415511.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX4849059.1"
                     /translation="MSTKRPFILGLTGSIGMGKSAVAAMFQGLGVPVFDADKAVHELQ
                     GPGGACLAPIERAFPGTTGPQGVDRQKLGAAVFGNPEALRLLESIVHPEVAEMRRAFL
                     ADNADQPLIVFDIPLLFEKTGNHGLDAVVVVSAPAAVQRERVLARPGMTAEKFEQILK
                     LQVPDAEKRARADHVIDTGTSLAETRHAVQRLVHQIGAKKN"
     gene            1864..2547
                     /gene="dnaQ"
                     /locus_tag="VFV30_13020"
     CDS             1864..2547
                     /gene="dnaQ"
                     /locus_tag="VFV30_13020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX4849060.1"
                     /translation="MREIIFDTETTGLDPKTGDRLVEIGCIEMINRVPTGNTFHAYFN
                     PDRDMPAAAEAVHGLSIAFLSDKPRFHERAAELIEFLGDSMLVAHNAGFDFGFVNAEL
                     EMCGMEPLSRERMIDTVAMARVRHPGAKNSLDALCTRYGIDRSHRTKHGALLDAELLA
                     QVYVELTGGRQIGLELAGSRSEELVVTVETVRRERVFRPARDHRPSEAELAAHREFLE
                     TVKDPLWNS"
     gene            2579..>3085
                     /gene="raiA"
                     /locus_tag="VFV30_13025"
     CDS             2579..>3085
                     /gene="raiA"
                     /locus_tag="VFV30_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX4849061.1"
                     /translation="MDIRVSGHQIETGEALQVHAQDRINAVIDKYFGQALSSTVTFNR
                     APAGAFRCDIVMHVMQGLLLKGAGIAQDAHVALDLAAAKVEKQLRRYKRRIKDRHEAA
                     SHAARAEEAAYTVFVEPDEEAEEIRADAPVVIAETRVDVPEATVSDAVMMLDLRNTNA
                     LLFKNAGTG"
BASE COUNT          532 a         1008 c         1017 g          528 t
ORIGIN      
        1 tcccggcagg ctggtgctgg gcagcgattc gctggtcgag gttgacgggc ggcggttcga
       61 caagccggcc agccgcgagg atgccgccgc gcatttgcgg ttcttctcgg gcaaggtcat
      121 gcagctccac agcgcggccg ccctggcgcg tgatggcggg gtgatctggc gtcatggtgc
      181 gctggcccgg ctcagggtgc gcgcgctttc ggagcagttc atcaccagct acctgggcca
      241 cgaatggccg gcggtggggg cctgcgtcgg ggtgttccgg atcgaggcgc gcggggtgca
      301 attgttcgag tcgatcgaag gtgatcattt cacggtgctt ggcatgccgc tgctgccggt
      361 actgggcgcg ctgcgccagc atggaaatct gctggcatga cgcgccctta tgccgaagtg
      421 atcggcgatc cgatcgcgca atccaaatcg ccggcgattc acaatttctg gctcgaaaga
      481 ctcgggatcg acgccgaata ccgcgcctgt catgtgacca cggaggggct ggcggactat
      541 gtcgccgagc gccgcggcga tccccaatgg cgcggctgca atgtgaccat gccgcacaag
      601 caggcagtga tggcgctgat cgacgcgatc gatccgcagg cggcgcgggt cggcgcggtc
      661 aacaccgtgg tgagcgtggc cgggcaaggg ttgacgggct tcaacaccga tgttgccggg
      721 tttctcgaac cgcttggcgg gcggccgttc gattgtgtca gcgtaatcgg cgccggcggc
      781 gcggcccgcg cgatccttgc cgcccttgcc gatcggggca cgcgctgggt ttcgctgcag
      841 aaccgcagca tcgccaaggg cgaggcactg ctggccgaat tcggccttgc gggctgcgtg
      901 gtcgaacccg gcgccgaagt tccgaccgcc gagcttctgg tcaacacttc gtcgctcggc
      961 atggcgggct ttccgccgct gcccgcggtc gaagccttcg tcatggatgg cggcacggtc
     1021 tatgacatcg tcactacccc gctcgaaacc gatctcatca ggcgcgcccg ggcccgcggc
     1081 cttgccacca tcgatggcct ggccatgctg atcggccagg cggcggcggc gttcgagaaa
     1141 ttcttcggcc agcccgcccc gcgcgggcac gatgccgaac tgcgcgcgct gctgacccga
     1201 tgagtacgaa gcgccccttc atccttggcc tcaccggctc gatcggaatg ggcaagagcg
     1261 cggttgcggc tatgtttcag ggcctgggcg tgccggtgtt cgatgccgac aaggcggtgc
     1321 acgaactgca gggtccgggc ggggcctgcc tggcaccgat cgaacgcgcc tttcccggca
     1381 ctaccgggcc gcaaggagtg gaccggcaga agctgggcgc ggcggtgttc ggcaaccccg
     1441 aggcgctgag gctgctcgaa agcatcgtcc accccgaagt tgccgaaatg cgccgcgcgt
     1501 tcctcgccga caacgccgat cagccgctga tcgtgttcga tattccgctg ctgttcgaga
     1561 aaaccggcaa ccatggcctt gatgcggtgg tggtggtttc ggccccggcc gcggtgcagc
     1621 gcgagcgcgt gctggcccgt ccgggcatga ccgccgaaaa gttcgagcag atcctgaaac
     1681 ttcaggttcc cgatgccgaa aagcgcgccc gcgccgacca tgtgatcgat accggcacct
     1741 cgctcgccga aacgcgccat gcggtgcagc gcctggtgca ccagatcggc gccaaaaaga
     1801 attagataaa gccgagtcgc ccccttgcca cccggccata acggtccgat atccttaagc
     1861 gagatgcgcg agattatctt cgataccgaa accaccggcc tcgatcccaa gactggtgac
     1921 cggctggtag aaatcggctg catcgaaatg atcaaccggg tccccaccgg caacaccttc
     1981 cacgcctatt tcaaccccga ccgcgacatg cccgccgcgg cagaggcggt gcacggcctg
     2041 tcgatcgctt tcctgtccga caagccgcgc tttcacgaac gcgctgccga actgatcgag
     2101 ttcctgggcg attcgatgct ggtggcgcac aatgccggtt tcgacttcgg gttcgtcaac
     2161 gccgagctcg agatgtgcgg aatggagccg ctctcgcgcg agcggatgat cgatacggtc
     2221 gccatggcca gggtgcgcca tcccggcgcc aagaactcgc tcgatgcact gtgcacccgc
     2281 tacgggatcg atcgcagtca ccgcaccaag cacggcgcgc tgctcgacgc cgaactgctt
     2341 gcccaggtct atgtcgagct taccggcggg cgccagatcg ggctcgaact ggctggatcg
     2401 cgcagcgagg aactggtcgt cacggtcgaa accgtcaggc gcgaacgcgt gttccgtccg
     2461 gcccgcgatc atcgtcccag cgaggccgaa cttgccgcgc accgggaatt tctcgaaacg
     2521 gtcaaggatc cgctctggaa cagctgaccg gttcgatttt tgaaggagaa ggccaaccat
     2581 ggatattcgc gtttcaggcc accagatcga aaccggcgaa gccctgcagg tgcacgccca
     2641 ggaccggatc aatgccgtga tcgacaagta tttcggccag gcgctttcct ctaccgtcac
     2701 cttcaaccgc gccccggccg gcgcctttcg ctgcgatatc gtgatgcacg tgatgcaggg
     2761 cttgctgctg aagggcgcgg gcatcgccca ggacgcccat gtcgcgctcg atctggctgc
     2821 ggccaaggtc gaaaagcagc tgcgccgcta caagcgccgg atcaaggatc gccacgaggc
     2881 ggcgagccac gccgcccgcg ccgaggaagc cgcctatacc gtgttcgtcg aaccggacga
     2941 ggaagccgag gaaatccgcg ccgatgcccc ggtggtgatc gccgaaaccc gggtcgacgt
     3001 gcccgaggcg accgtttccg acgcggtgat gatgctcgat ctgcgcaaca ccaacgcgct
     3061 gctgttcaag aatgccggca ccggc
//
LOCUS       DASUDV010000589         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_219703,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000589 DASUDV010000000
VERSION     DASUDV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219703"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFV30_13030"
     CDS             complement(<1..579)
                     /locus_tag="VFV30_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEX4849062.1"
                     /translation="MTFTGKQLTTQLDADGTLTVQLNDKTWDAPQGSQVLIKVEASPI
                     NPSDLGLLYASADMDNAVYSPGKVVAQMPPPATRAFAARHGMAMPAGNEAAGIVVAAG
                     EDPAAQALMGKRFACVPGSAYATYAYADANMGFAVDEAISADQAASSFVNPMTALGFT
                     ETMKMEGFTGLVHAAAASNLGQMLVKICQEDNI"
     gene            complement(622..1368)
                     /gene="truA"
                     /locus_tag="VFV30_13035"
     CDS             complement(622..1368)
                     /gene="truA"
                     /locus_tag="VFV30_13035"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX4849063.1"
                     /translation="MTRFALTLEFDGTPFMGLQRQAHGPSVQQAVEEAVLATTGETVT
                     LHAAGRTDAGVHALGMRVHVDVAKEIDAFRLMEALNYHLRPAPIAVLDCAVVADDWHA
                     RFSCIGREYVYRIANRRAPLTLDANRAWQVPQPLDAEAMHRAAQALVGRHDFTTFRSV
                     HCQAQSPEKTLDLLSVERVGEEVRIRAAARSFLHHQVRSMVGCLALVGMGRWRERQVA
                     EALAARDRQALGLNAPSEGLYFVRALYPGE"
     gene            complement(1365..2273)
                     /gene="fmt"
                     /locus_tag="VFV30_13040"
     CDS             complement(1365..2273)
                     /gene="fmt"
                     /locus_tag="VFV30_13040"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX4849064.1"
                     /translation="MRVIFMGTPDFAVPALQALVQVGHEVVCAYTQPPRPGGRRGKEL
                     TPTPVHKAAEALGIEVRHPVSLKGADEQAHFAALGADVAVVAAYGLILPQAVLDAPRH
                     GCLNIHASLLPRWRGAAPIQRAILAGDAETGVTIMQMERGLDTGPMLAVVKTPVDAKT
                     TGELTGELAELGASLMVEVLADLPGHAAVVQPEAGVTYAHKIDKAEARIDWTRSAEEI
                     ERQVRAFAPAPGAWFEYQGERCKVLTAELAEGTGQAGLVLDDKLTVACGKGAIRPTLV
                     QRAGRPAIATAEMLRGWSIEAGTKFA"
     gene            2317..>2759
                     /locus_tag="VFV30_13045"
     CDS             2317..>2759
                     /locus_tag="VFV30_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX4849065.1"
                     /translation="MASQEIETLTAALSRLPGLGPRSARRAVLWLIKRRETSLVQLLA
                     ALDGVAERLVECETCGNIDTTNPCGLCVDPRRDARQLCVVEDVADLWALDRARLFTGK
                     YHVLGGRLSALEGVRPEDLTIARLIERVEAGGIDEVVLAMNATLEG"
BASE COUNT          435 a          929 c          915 g          480 t
ORIGIN      
        1 gatgttgtct tcctggcaga tcttgaccag catctggccc aggttcgaag cggccgcggc
       61 atggaccagg ccggtgaagc cttccatctt catggtttcg gtaaaaccaa gcgcggtcat
      121 ggggttgacg aacgaacttg cagcctggtc tgcactgatc gcctcgtcca ccgcaaagcc
      181 catattggca tcggcatagg cataagtggc ataggcgcta cccggcacgc aggcaaagcg
      241 cttgcccatc agcgcctggg ccgccggatc ctcgccagcg gccacaacga tgcccgccgc
      301 ctcgttgcca gcgggcatcg ccatgccgtg gcgcgcggca aaggcacggg tggcgggcgg
      361 cggcatctgc gccaccacct tgcccggcga atagacggca ttgtccatat cggccgaggc
      421 atagagcagg ccaaggtccg acgggttgat cgggcttgcc tcaaccttga tcagcacctg
      481 gctgccttgg ggcgcgtccc aggtcttgtc gttgagctgg acggtcagcg tgccgtcggc
      541 atcaagctgg gtggtgagtt gcttgccggt gaaggtcatt ggtcgtctcc cgaatcctgt
      601 ttcggaatgc aacctagtga ctcattcgcc ggggtaaagc gcccgcacga aatagagccc
      661 ttcgctgggg gcattgaggc ccagcgcttg ccggtcgcgc gcggccagcg cctcggccac
      721 ctggcgctcg cgccaccgcc ccatgccgac cagcgccaga cagccgacca tcgagcggac
      781 ctggtggtgg aggaagctgc gcgcggcagc gcggatccgc acttcttcgc ccacgcgctc
      841 gacgctgagc aggtcgagcg tcttttccgg gctctgcgcc tggcagtgga ccgaacggaa
      901 cgtggtgaaa tcgtgccgcc ctaccagtgc ctgcgccgcg cggtgcatgg cctcggcatc
      961 gagcggctgg ggcacctgcc aggcgcggtt tgcatccagc gtcagcggcg cgcggcggtt
     1021 ggcgatgcgg tagacatatt cgcggccaat gcacgaaaag cgcgcgtgcc agtcatccgc
     1081 caccactgcg cagtccagca ccgcgatcgg tgcagggcgc aggtgatagt tgagcgcttc
     1141 catcagccgg aaggcatcga tctctttggc cacatcgaca tggacccgca tccccagtgc
     1201 gtgcaccccg gcatcggtgc gtccggcggc gtggagcgtg acggtttcgc cggtcgttgc
     1261 cagcaccgct tcctccaccg cctgttgcac cgaagggcca tgtgcctggc gctgcagccc
     1321 catgaacgga gtgccatcga attcgagggt gagggcgaaa cgggtcaagc gaacttcgtc
     1381 ccggcttcga ttgaccaccc gcgcagcatt tccgctgtcg ctattgctgg tcgtcccgcg
     1441 cgctggacga gagttgggcg gatcgcaccc ttgccgcagg cgactgtcag cttgtcatca
     1501 agcacgaggc cagcttgacc tgttccctca gccagctctg ccgtcagcac cttgcagcgt
     1561 tcgccctggt actcgaacca ggcgcccggc gcgggggcaa aggcgcgcac ctggcgttcg
     1621 atttcctcgg cactgcgcgt ccagtcgatg cgggcttcgg ccttgtcgat cttgtgggca
     1681 taggtcaccc ccgcctcggg ctggaccact gccgcatggc ctggcaaatc agccagcacc
     1741 tcgaccatca ggctggcgcc cagttcggcc agttccccgg tcagttcgcc ggtggtcttg
     1801 gcatcgaccg gggtcttgac cacggcgagc atcggcccgg tatcgaggcc tctttccatc
     1861 tgcatgatgg tgaccccggt ttcggcatcg cctgccagga tcgcgcgctg gatcggcgcg
     1921 gcgccgcgcc agcgcggcaa cagcgaggcg tggatgttga ggcagccatg ccgcggcgca
     1981 tcgagcaccg cctgcggcag gatcaggcca taggcggcaa ccaccgcgac atcggccccc
     2041 agcgctgcga agtgcgcctg ctcgtccgcg cccttcagcg aaaccggatg gcgcacctcg
     2101 atccccagtg cctcggccgc cttgtgcacc ggggtagggg tcaactcctt gccgcgccgc
     2161 ccaccggggc gggggggttg ggtataggcg caaaccactt catgtcccac ctgcaccagc
     2221 gcctgcagcg ccggcacggc aaaatccggg gttcccatga agatgacacg catcgtggct
     2281 ttgcctttcc tgaaaccgcg ccctatctgt caggccatgg catcgcaaga gatcgaaacg
     2341 cttaccgcag ccctttcgcg cctgcccggg cttggcccgc gctcggcgcg gcgggcggtg
     2401 ctgtggctga tcaagcggcg cgaaacctcg ctggtgcaac tgctcgccgc gctggacggc
     2461 gtggccgaac ggctggtgga atgcgaaacc tgcggcaaca tcgataccac caatccctgc
     2521 gggctctgcg tcgatccgcg ccgcgatgcc cgccagttgt gcgtggttga ggatgtcgcg
     2581 gacctctggg cgctcgaccg ggcgcggctg ttcaccggca agtaccacgt gctgggcggg
     2641 cggctttccg cgctggaagg cgtgcggccc gaagatctca ccatcgcccg gctgatcgag
     2701 cgggtcgagg ccggcgggat cgacgaggtg gtgctggcga tgaacgccac gctggaggg
//
LOCUS       DASUDV010000590         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_904582,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000590 DASUDV010000000
VERSION     DASUDV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904582"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFV30_13050"
     CDS             <1..588
                     /locus_tag="VFV30_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4849066.1"
                     /translation="REESDARLLAAAAGVIAREGYLAATLERVGTEAGFSRGLASRKY
                     GSKDGLIEAVIRHTSTHVHAELDKAVAGVVSPLDQLVALFERFVALVGSDDLVRAYFV
                     LFSAMIANRLETRLVFDEVQQRFGQRLQDLIERAQRTGAIAPALPARHGAYLVGCLLA
                     GIAVETTMDSPPPVAVDAARLQADLSAMLRRALGG"
     gene            641..1912
                     /locus_tag="VFV30_13055"
     CDS             641..1912
                     /locus_tag="VFV30_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4849067.1"
                     /translation="MLRPVLAALLLSVSAPALAETVYVRAGHLVDPEAGKVWDDRLIR
                     IEDGRVAAVTPFAPPPAGAQLIDWSGKWVLPGLIDMHVHLADIGQSNNVAEPLLHSQG
                     EIAYVGARNARTTLNAGFTTVHDVGSFRGLANIELRNAIDRGDVPGPRISATGAYITI
                     PGGGGEVTGLAADTVLPPDMRLGVAATPSEVEQRVNYLFQHGADSIKLIATGAVLAEG
                     TEPGQQELSEEMMSAAVRVAAQRKAFVTAHAHGAEGIKAAIRSGVHSIEHASLIDDEG
                     IQLAKARGVYLVMDIYNGDFISEVGRRDGWPAAMLRKNDETTDAQRQGFARAVKAGAR
                     ISFGTDAGVYPHGLNARQMRYMVRYGMSPMQAIQAATTVAAEQLGWSKDVGAISPGRF
                     ADMIAVSADPLKDITVLEKVDHVIKGGELVR"
     gene            1933..>2975
                     /locus_tag="VFV30_13060"
     CDS             1933..>2975
                     /locus_tag="VFV30_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4849068.1"
                     /translation="MKRLALALLLATALPAAPAALAQANPSPAQTWIHAGRVIAVPGQ
                     APRGASTIVVADGRIVAIHDGYLPAPAGSQAIDLKGRTVLPGLIDSHTHLSSDRAGLE
                     ARAAWVSDSMPLLAYETYWNGMKTLHAGFTTVRNLGDDGGKTLALKEAIKRGWVQGPR
                     IIDAGASISTTGGHMDGRVGVPDDIGHELVSTENLCDGADDCRRAVRRQIGRGADVIK
                     FATTGGVNSGTGLATRMIEEEAKALVETAHAYGRKVAVHAHGLDGIKLAVRAGADSIE
                     HGTTIDEETAKMMAKAGTYYVPTLSTVNGYLERLAANPNAYSPAVRAQIDWRIGITGK
                     SLQIAYPLGVKIA"
BASE COUNT          511 a          993 c         1007 g          464 t
ORIGIN      
        1 cgcgaggaat ccgacgcccg gctgctggcc gcggcggccg gggtgatcgc gcgcgagggc
       61 tatcttgccg ccacgctcga acgggtcggg accgaggcag gcttttcgcg cggactggcc
      121 agccgcaagt atggatcgaa ggacgggctg atcgaggcgg tgatccgcca cacctcgacc
      181 catgtccatg ccgaactcga caaggcggtg gccggcgtgg tcagtccgct cgatcagctg
      241 gtcgcgctgt tcgaaaggtt tgtcgcgctg gtcggctcgg acgatctggt gcgcgcctac
      301 ttcgtgctgt tcagcgcgat gatcgccaac cggctggaaa cccgcctggt gttcgacgag
      361 gtgcagcagc gctttggcca gcggcttcag gacctgatcg agcgtgccca gcgcacaggg
      421 gcgatcgccc ccgccctgcc cgcgcggcat ggcgcctatc tggttggctg cctgctggca
      481 gggatagcgg ttgaaaccac gatggacagc ccgccgccgg tggcggttga tgccgcccgg
      541 ctccaggccg atctctcggc catgctgcgc cgcgccctcg ggggctagcc attcgggcgg
      601 acaggcgcca ctctggccca aaccgccaag ggattcgccg atgctccgcc ccgttcttgc
      661 cgccctgctg ctttcggttt ccgcccccgc cctggctgaa acggtctatg tccgggccgg
      721 gcacctggtc gatcccgaag cgggcaaggt ctgggacgac cggctgatcc ggatcgagga
      781 cgggcgggtt gccgcggtca cgccctttgc tccgccgccc gccggggcgc agctgatcga
      841 ctggtcaggc aaatgggtcc tgccgggcct gatcgacatg cacgtgcacc tggccgacat
      901 cggccagtcg aacaacgtgg ccgaaccgct gctccattcg cagggtgaaa tcgcctacgt
      961 tggcgcgcgc aacgcccgca ccacgctcaa tgccgggttc accactgtcc atgatgttgg
     1021 cagtttcagg gggctggcca acatcgaact gcgcaacgcg atcgatcgcg gcgatgttcc
     1081 cgggccgcgg atcagcgcga ccggcgccta tatcaccatc cccggcggcg gcggcgaggt
     1141 gaccgggctt gctgctgata ccgtcctgcc gccggatatg cggctggggg tggcggccac
     1201 gccttcggag gttgagcaac gggtcaacta tctgttccag cacggcgcgg attcgatcaa
     1261 gctgatcgcg accggtgcgg tgctcgccga aggaaccgaa cccggccagc aggaattgtc
     1321 ggaagagatg atgagcgccg cagtgcgggt agccgcgcag cgcaaggcct tcgtcaccgc
     1381 ccacgcccac ggtgccgaag ggatcaaggc ggcaatccgc tcgggcgtgc attcgatcga
     1441 gcatgccagc ctgattgatg acgaggggat ccagttggcc aaggcacgcg gggtctatct
     1501 ggtgatggat atctacaacg gtgatttcat cagcgaagtc ggccggcgcg atggctggcc
     1561 cgcggctatg ctgcgcaaga acgatgaaac caccgatgcc cagcgccagg gttttgccag
     1621 ggcggtcaag gcgggggcgc gaatctcgtt cggaaccgat gccggggtct acccccacgg
     1681 gctcaatgcc aggcaaatgc gatacatggt gcgctatggg atgagcccga tgcaggcgat
     1741 ccaggcggcc accacggtcg ccgccgaaca gctggggtgg agcaaggacg tgggcgcgat
     1801 cagcccgggc cgctttgccg acatgatcgc ggtgagcgcc gatccgctga aagacatcac
     1861 cgtgctggaa aaggttgacc acgtgatcaa aggcggcgaa ctcgtccgct gaaactggag
     1921 agaatgttcc cgatgaagcg tcttgccctt gccctgctcc tggccactgc cctgcccgcc
     1981 gcccccgccg cgctggcgca agccaaccct tccccggcgc agacctggat ccacgccggg
     2041 cgggtgatcg ccgtgcccgg ccaggccccg cgcggggcat cgaccattgt cgttgccgac
     2101 ggcaggatcg tggcgatcca cgatggctat ctgcccgcgc ccgccggatc gcaagcgatc
     2161 gacctcaagg gccgcaccgt gctgcccggc ctgatcgaca gccacaccca cctttcctcg
     2221 gaccgggcgg ggctggaagc gcgcgccgcc tgggtttcgg attcgatgcc gctgctggcc
     2281 tatgaaacct actggaacgg gatgaagacg ctccacgccg gcttcaccac ggtgcgcaac
     2341 ctgggcgacg atggcggcaa gaccctggcg ctcaaggaag caatcaagcg cggctgggtg
     2401 cagggcccgc ggatcatcga tgccggcgcc tcgatctcca ccaccggcgg ccacatggac
     2461 gggcgcgtcg gcgtgcccga cgacatcggc catgagctgg tcagcaccga aaacctgtgc
     2521 gacggggccg acgattgccg ccgcgcggtg cgccgccaga tcggccgcgg cgccgatgtg
     2581 atcaagttcg ccaccaccgg cggggtcaat tcgggcacgg gcctggccac ccggatgatc
     2641 gaggaagaag ccaaggccct ggtcgaaacc gcccatgcct atggccgcaa ggtcgcggtc
     2701 catgcccacg ggctagatgg gatcaagctg gcagtgcgcg ccggggcgga ttcgatcgaa
     2761 cacggcacca cgatcgatga agagaccgcg aaaatgatgg ccaaggccgg cacctactat
     2821 gtccccacgc tcagcaccgt gaacggctat ctcgaacggc tggccgccaa tcccaacgcc
     2881 tattcgccgg cagtccgggc gcagatcgac tggcggatcg ggatcaccgg caagagcctg
     2941 cagatcgcct atccgctggg cgtgaagatc gccta
//
LOCUS       DASUDV010000591         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_639933,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000591 DASUDV010000000
VERSION     DASUDV010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639933"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..835
                     /locus_tag="VFV30_13065"
     CDS             8..835
                     /locus_tag="VFV30_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX4849069.1"
                     /translation="MAVINTNISAIKAANASNSANKMLGTAMERLSTGKRINSAKDDA
                     AGLAIATSMTAQVRGMNQAIRNSNDGIALAQTAEGALNEVTNMLQRVRELAVQSASGT
                     YQDATDRAYMQSEVDELTAQIDQVITNSKFNGVQLFDGTTATVTVQTGADGADTVDLT
                     MADLTSLAASGGAAGSYDVSSATAANGLLTTIDGELDSINSARSSLGAGQNRLESVVN
                     NLTSNSTNLSDARSRIEDADYSAETTAMAKAQILSQASTAMIAQANQSQQNVLSLLR"
     gene            1036..1704
                     /locus_tag="VFV30_13070"
     CDS             1036..1704
                     /locus_tag="VFV30_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849070.1"
                     /translation="MAGFLLALAPLPAWGQAAPAPAAPKAAPRAAPPAAAPLPGNTVR
                     CVYEYLSPEDREIALLLLGREMIDGGNFTDASKNIQVVNRLLAEAEAKCLDRFNWSIG
                     RSKSATNWALTQILSEALVQALDYAGSKPADVDAYYAENRPLLRGKRAIEGADKEALN
                     AYLIEKGWDAESKAQLGLAAIYLETYVMKDSDERWFQYSGGSTRQPSPRSPSRAKKGR
                     RGRP"
     gene            complement(1652..2962)
                     /locus_tag="VFV30_13075"
     CDS             complement(1652..2962)
                     /locus_tag="VFV30_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849071.1"
                     /translation="MIRPAVPILLAAALALGWTAPGHAEDGWPVLDKPTRPAKPADKK
                     DTLVPAKVFLSEDGQTVYIIGMIVDGTFHDFDGVLQKAPRARTVYLASGGGYTLEARL
                     IAAMVRKRGLNTYVEFYCASACTQVFAAGKERVLGPTARMGFHQAVRIDSRGRPIGVR
                     AKTDRKLTPTTVFGVNGNDTLRLAYELAGTDPAFIEKALNQSPDGMWLPTRNEVLAAR
                     MATRLATTSEVAAPPGGGGTREEVRARMLKEPLWRAVLARKPDLAEAVFEDTYFLVNS
                     GIEWTEAVATGRGRAVKLAMQRLARSPDPVLEQALTYYALSARYQRERGWPACAAMVG
                     KPSKPLVAEDYEMLAQEDALMVTILDTEEQVPAMDGEAATKVFSKDVAPTLVPAYKAS
                     LRAGTEGKCRLGLQTFEVVGALPAKKRIKAYRAVLSLPGMATAD"
     gene            complement(2994..3773)
                     /locus_tag="VFV30_13080"
     CDS             complement(2994..3773)
                     /locus_tag="VFV30_13080"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX4849072.1"
                     /translation="MDSPLSRSGIWHGSARRKVEAGARLCEACAMRIAFLLFPGLTQL
                     DLTGPAQFLSRLPDARVDLVWENRDPVPTDAGFSILPTATFADIADTDLLCVPGGMGI
                     AKVIDHAPALDWVRQVGGSATWVTSVCTGALVLGAAGLLRGYRATTHWAWHDQLALFG
                     AQPVQARTVVDHNRVTGGGVTAGIDFALTLMAEVAGPDTARLVQLALEYDPAPPFDSG
                     SPAKAGAELIAAYQARAARLAPGRLEELRAAAVRLGFIAAD"
     gene            3772..>4671
                     /locus_tag="VFV30_13085"
     CDS             3772..>4671
                     /locus_tag="VFV30_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX4849073.1"
                     /translation="MTRYTGAIDQGTTSTRFIVFDHAGAIISVAQKEHAQIMPQPGWV
                     EHDADEIWSNTREVIGAALAKAELTPADLAAVGITNQRETSVLWDKATGQPVHNALVW
                     MDTRTEALVAAFEAEGGQDRFRAKTGLPLATYFSGLKLRWLLDNVPGARARAEAGELL
                     FGNIDTWLAWNLTGLHITDVTNASRTQMMDLETLDWDPEILAAFGVPRACLPQIRSSS
                     EVYGTCTGVLDGVPLAGILGDQQAALFGQACLAPGEAKNTYGTGCFMLMNTGEKPFPS
                     QAGLLTTLGYKLGDAPAVYALEGS"
BASE COUNT          785 a         1634 c         1570 g          682 t
ORIGIN      
        1 taccaccatg gctgttatca acaccaacat cagcgccatc aaggcggcca acgcttcgaa
       61 ctcggccaac aagatgctcg gcaccgcgat ggagcgcctg tcgaccggca agcgcatcaa
      121 cagcgccaag gacgacgccg ctggcctggc gatcgccacc agcatgaccg cgcaggtgcg
      181 cggcatgaac caggcgatcc gcaactcgaa cgacggcatt gccctggccc agaccgccga
      241 aggtgcgctg aacgaagtca ccaacatgct gcagcgcgtg cgcgaactgg ccgtccagtc
      301 ggcctcgggc acctatcagg acgccaccga ccgcgcctac atgcagtcgg aagtggacga
      361 gctgaccgcg cagatcgatc aggtcatcac caacagcaag ttcaacggcg tgcagctgtt
      421 tgacggcacc accgccaccg tcacggtcca gaccggtgcc gacggggccg acaccgttga
      481 cctgaccatg gccgacctga cctcgctggc tgccagcggc ggcgcggcgg gttcctatga
      541 cgtgtcttcg gcgacggccg ccaacggcct gctgaccacg atcgacggcg aactcgacag
      601 catcaactcg gcccgctcct cgctgggtgc cggccagaac cggctggaat cggtggtcaa
      661 caacctgact agcaattcga ccaacctcag cgacgcgcgc agccggatcg aggacgccga
      721 ctattcggcc gaaacgaccg cgatggccaa ggcccagatc ctctcgcagg cttcgaccgc
      781 gatgatcgcc caggccaacc agagccagca gaacgtcctg tcgctgctgc gctaaggctt
      841 ctggttcccc tcctccccaa gcggagggga gccgccaagc ccttgcccgg ctgcctgtcc
      901 cttccaggca tggcccggcg gtcacccgac cgccgggcct ttggcatggg cgagcggcct
      961 ctcgacagat cgtccggcac acggcagatt gaacccgaac cgcggagaca aggcgtgcgc
     1021 gcaagacatt gggcaatggc gggatttctg ttggcgctgg cgccgctgcc ggcctggggc
     1081 caggccgccc cggcgccagc cgcgcccaag gccgcacccc gcgccgcgcc gcccgccgcc
     1141 gcgccgctgc cgggcaacac cgtgcgctgc gtttacgaat acctctcgcc cgaagaccgt
     1201 gagatcgccc tgctgctgct ggggcgcgag atgatcgacg gcggcaactt caccgatgcg
     1261 tcgaagaaca tccaggtcgt caaccggctg ctggccgagg ccgaggccaa gtgcctggac
     1321 cggttcaact ggtcgatcgg ccgttccaaa tccgccacca actgggccct gacccagatc
     1381 ctgtccgaag cgctggtcca ggcgctggac tatgccggca gcaagcccgc cgatgtcgat
     1441 gcctattacg ccgagaaccg cccgctgctg cgcggcaagc gcgcgatcga gggggccgac
     1501 aaggaggcgc tgaacgccta tctgatcgaa aagggctggg acgcggagag caaggcccag
     1561 cttggcctgg ccgcgatcta tctggaaacc tatgtcatga aggacagcga cgaacgctgg
     1621 ttccagtaca gcggcggatc cacccgccag cctagtccgc ggtcgccatc ccgggcaaag
     1681 aaaggacggc gcggtaggcc ttgatccgct ttttcgcggg cagggcgccg accacttcga
     1741 aggtttgcag gccaaggcgg cacttgcctt cggtgccggc gcgcaggctg gccttgtagg
     1801 ccgggaccag ggtgggggcg acgtccttgg aaaacacctt ggtcgccgct tcgccgtcca
     1861 tcgccgggac ctgctcctcg gtatcgagga tggtcaccat cagggcgtct tcctgggcca
     1921 gcatttcata gtcttcggca accagcggct tgctgggctt gccgaccatg gccgcgcagg
     1981 ccggccagcc gcgctcgcgc tggtaacggg ccgacagggc gtaataggtc agcgcctgct
     2041 ccagcaccgg atcgggcgag cgggccagcc gctgcatcgc cagcttgacc gcccggccgc
     2101 gcccggtggc gaccgcctcg gtccattcga tcccgctgtt gaccaggaaa taggtgtctt
     2161 cgaacacggc ttcggccagg tccggcttgc gggccaggac cgcgcgccac agcggctcct
     2221 tcagcatccg ggcgcgcacc tcttcgcggg ttccgccgcc gccgggcggg gccgccactt
     2281 cgctggtcgt cgccagccgg gtggccatcc gcgccgccag cacttcgttg cgcgtcggca
     2341 gccacatccc gtcgggcgac tggttgagcg ccttttcgat gaaggccgga tcggttccgg
     2401 ccagttcata ggccagccgc agcgtgtcgt tgccgttcac cccgaacacg gtggtcgggg
     2461 taagcttgcg gtcggtcttg gcgcgaaccc cgatcggccg cccgcgcgaa tcgatgcgca
     2521 cggcctggtg aaagcccatc cgcgcggttg ggccgagcac ccgttccttg cccgccgcga
     2581 agacctgggt gcaggccgag gcgcagtaga actcgacata ggtgttgagc ccgcgcttgc
     2641 ggaccatggc cgcgatcagc cgcgcttcca gcgtatagcc cccgcccgac gcgaggtaga
     2701 ccgtccgggc ccgcggcgcc ttttgcagca cgccgtcaaa atcgtgaaag gtgccgtcca
     2761 cgatcatgcc gatgatgtag accgtctggc cgtcttccga gaggaacacc ttggccggaa
     2821 ccagcgtgtc cttcttgtcg gccggtttgg ccggccgggt tggcttgtcg agcacgggcc
     2881 agccgtcttc ggcatggccg ggcgcggtcc agcccagggc cagtgcggca gccagcagga
     2941 tcggcacggc ggggcggatc atcgggggat catgccggat aaaggctgcg cgttcaatcc
     3001 gctgcgataa agcccagccg cacggccgcg gcgcgcagtt cctccagccg cccgggggcc
     3061 agccgcgcgg cccgcgcctg gtaggcggcg atcagttccg caccggcctt ggccgggctg
     3121 ccgctgtcga acggcggggc gggatcgtat tccagcgcca gctggaccag ccgggcggtg
     3181 tccggtccgg ccacctcggc catcagggtc agggcaaagt cgatcccggc ggtcacgccg
     3241 ccgccggtta cccggttgtg gtccaccacg gtgcgggcct ggaccggctg tgccccgaac
     3301 agggccagct gatcgtgcca ggcccagtgg gtggtcgcgc ggtagccgcg cagcagtccg
     3361 gccgcgccca gcaccagcgc gccggtgcag acgctggtca cccacgtcgc gctgccgccg
     3421 acctgccgca cccaatcgag cgccggggca tggtcgatca ccttggcgat tcccatgccg
     3481 ccgggcacgc acagcaggtc ggtatcggca atatcggcaa aggtggcggt cggcaggatc
     3541 gagaagccgg cgtcggtcgg caccggatcg cggttttccc agaccagatc gacccgggca
     3601 tcgggcaggc gcgagaggaa ctgggccggg ccggtcaggt ccagctgggt caggccggga
     3661 aacagcagaa aggcgatgcg catggcgcag gcttcgcaca gccgggcacc ggcttcaacc
     3721 ttgcggcggg ccgatccgtg ccatatcccg ctgcgcgaga gaggagaatc catgacccgc
     3781 tataccggtg cgatcgacca ggggacgacc agcacgcgtt tcatcgtgtt cgaccacgca
     3841 ggcgcgatca tctcggtcgc gcagaaggag cacgcccaga tcatgcccca gcccggctgg
     3901 gtggagcacg acgcggacga gatctggagc aacacccgcg aagtgatcgg cgcggcgctg
     3961 gccaaggccg agctgacccc ggcagacctg gccgcggtgg gcatcaccaa ccagcgcgaa
     4021 accagcgtgc tgtgggacaa ggcgaccggc cagccggtgc acaacgcgct ggtctggatg
     4081 gacacccgca ccgaagcgct ggtcgccgcg ttcgaggccg agggcgggca ggaccggttc
     4141 cgcgccaaga ccgggctgcc gctggcgacc tatttctccg gtctcaagct gcgctggctg
     4201 ctcgacaacg tgcccggcgc gcgcgcccgg gccgaagcgg gcgagctgct gttcggcaat
     4261 atcgacacct ggctggcctg gaacctgacg ggcctgcaca tcaccgatgt caccaacgcc
     4321 tcgcgcaccc agatgatgga tctggaaacg ctggactggg acccagagat tctggccgcg
     4381 ttcggcgtgc cgcgcgcctg cctgccgcag atccgcagct ccagcgaagt ctatggcacc
     4441 tgcaccggcg tgctggacgg tgtgccgctg gccgggatcc tgggcgatca gcaggccgcg
     4501 ctgttcggcc aggcctgcct ggccccgggc gaggccaaga acacctatgg caccggctgc
     4561 ttcatgctga tgaacaccgg ggaaaagccg ttcccctcgc aggccgggct gctcaccacg
     4621 ctgggctaca agctgggcga tgccccggcg gtctatgcgc tggaaggctc g
//
LOCUS       DASUDV010000592         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1611487,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000592 DASUDV010000000
VERSION     DASUDV010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611487"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFV30_13090"
     CDS             complement(<1..581)
                     /locus_tag="VFV30_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX4849074.1"
                     /translation="MNGLTFGFGALEAELWRLVFVMTRIGAALLAAPFFSAASVPVQL
                     RIGITGAVAVLVCAWLPVEAPAALLSTAGFLAIAGEVLIGLALGFVLQLAFTAPVMAA
                     EQIGGAMGMSIAAAADPVSGAHSPVLGQYFMVVLTLVFLGLGGHLVFLRLVVESYQAF
                     PPGAAWLGTDRLGLVASFATDMLVAALAIALPVT"
     gene            complement(578..850)
                     /locus_tag="VFV30_13095"
     CDS             complement(578..850)
                     /locus_tag="VFV30_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX4849075.1"
                     /translation="MDEIAPLLALADRMLWVTALVAAPVLLASLAVGLVVGVIQAATS
                     VNEQTLTFVPKLAVIALVMVVLGASMLGLVADFTQDIFAEIARLGQ"
     gene            complement(856..1599)
                     /gene="fliP"
                     /locus_tag="VFV30_13100"
     CDS             complement(856..1599)
                     /gene="fliP"
                     /locus_tag="VFV30_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012324.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX4849076.1"
                     /translation="MRRLLAFPALLLPVAAHAQSAIPGALDRAFSTMGGSQGPGMTLS
                     LQLLVIMGLLTMLPALVLMMTSFTRILVVLSILRQALGLQQSPPNQVLIGLSLFLSLF
                     VMAPTLERVNAQAIAPYAAGTINAEQAIASAGGEFHAFMIRQTREKDLAMFAEMAKAP
                     RFARSQDVPFSILLPAFVTSELKTAFQIGFMLFLPFLVIDLVVASVLMSLGMMMMSPT
                     IVSLPFKLLLFVLVDGWALLMGSLVASFG"
     gene            complement(1596..1865)
                     /locus_tag="VFV30_13105"
     CDS             complement(1596..1865)
                     /locus_tag="VFV30_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX4849077.1"
                     /translation="MIWYLIKLIVLLPLIGAAAWGTLKLAQRLQARIAGSQSAARSVR
                     IIETTMLSPTLKLAVIEFHGREILVSASRQGLTRLAEAPAREAGQ"
     gene            complement(1862..2137)
                     /gene="fliN"
                     /locus_tag="VFV30_13110"
     CDS             complement(1862..2137)
                     /gene="fliN"
                     /locus_tag="VFV30_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX4849078.1"
                     /translation="MTLHTRGFDLIKEVDVRLSVELGRAEMKLRDVLALGEESVIMLD
                     RLTDELLDVMVNGKLIARGEIVAQGGRFGLRIVELAGADTETQAGAA"
     gene            complement(2150..3034)
                     /locus_tag="VFV30_13115"
     CDS             complement(2150..3034)
                     /locus_tag="VFV30_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX4849079.1"
                     /translation="MKPERAFTAERIAAQHCPELLQRATGCEPADLLGDFTRMGDRLA
                     RLLAPELARLLGSEEPVVHVSAARELSEGELASELGPLAANSLIASGVPGIALLASLE
                     GSGLLRLVDRAFGGKGDAPAVMPKVFPLSASLMIDRIEALLISALGGAMGQDGLKALR
                     RDTSLAELAPFPAGARLALLSCEVAAGARAPWKFTIALPLVSLPRLFGNAAATPREPR
                     SADPAAEPFATMPLTLVATLVDMAVPISRLSALEPGTVLPVAVARAVPITLGTATLAR
                     GTVGAQDDRIAIKLTQIA"
     gene            complement(3031..3276)
                     /locus_tag="VFV30_13120"
     CDS             complement(3031..3276)
                     /locus_tag="VFV30_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX4849080.1"
                     /translation="MSLACSTRRDGRVLMWLKKHELAIRSEILVVLADTPEEDAFTPD
                     GKQRLQKRMTDAINKVLVKTEGFGGVDQVYFKSYLVQ"
     gene            complement(3234..3623)
                     /locus_tag="VFV30_13125"
     CDS             complement(3234..3623)
                     /locus_tag="VFV30_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849081.1"
                     /translation="MSDSNEAEAKPRKKKGKGFLIKGLIGLILIAAGGGGTFALVQAG
                     MLGDHAEEKKEADHPKLVKKGEEDPYAPKTEAKEGEGPPKSRARAAANTAPAITTSPR
                     TLPRTSGIRARSSRSAWPVRPGAMAGC"
     gene            complement(3653..>4092)
                     /locus_tag="VFV30_13130"
     CDS             complement(3653..>4092)
                     /locus_tag="VFV30_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849082.1"
                     /translation="PSLQTDAAPRPHDFAGLIDRLVAARDAVQPQAVSIAVQHADFGE
                     ISLRLRHDGEALSISLASADPDFARIASTATPPVLQALAAEAPGQGNARPEQNATGNA
                     GTGGSTAQSRGGSPERRDDQPRQQPRPEHRAPERGNPRGGIFA"
BASE COUNT          744 a         1348 c         1336 g          664 t
ORIGIN      
        1 gtgaccggca gggcgatcgc aagtgcggca accagcatgt cggtggcaaa gctcgccacc
       61 aggcccagcc ggtccgtgcc cagccacgcc gcgccgggcg ggaaggcctg atagctttcc
      121 accaccagcc gcaggaacac cagatgcccg ccaagaccca ggaagaccag ggtcagcacg
      181 accatgaagt attggccgag caccggcgaa tgtgcaccgc tgaccggatc ggcagcggcg
      241 gcaatgctca tccccatggc cccgccgatc tgttccgcag ccatcaccgg ggcagtgaag
      301 gccagttgca ggacaaagcc cagcgccagc ccgatcagca cttcgccggc gatggcgagg
      361 aacccggcgg tcgacagcag cgctgccggc gcttcgaccg gcagccaggc gcagaccagc
      421 accgcgaccg cgccggttat cccgatcctg agctgcacag gaacactggc agcgctgaag
      481 aacggagcgg cgagcagcgc ggcgccgatc cgggtcatca cgaagaccag ccgccacagt
      541 tccgcctcaa gcgcgccgaa gccgaaggtc aagccgttca ctgacccagc cttgcgatct
      601 cggcaaagat gtcctgggtg aaatcggcga ccagccctag catcgatgcc cccagcacga
      661 ccatgaccag ggcaatcacc gctaacttgg ggacgaaggt cagggtttgt tcgttgaccg
      721 aagtggcggc ctggatcacg ccgacgacca ggcccacggc caggctggcc agcagcaccg
      781 gcgcggcgac cagcgcggta acccacagca tccggtcggc cagcgcgaga agcggggcaa
      841 tttcgtccat cgcggctacc cgaagcttgc cacaaggctg cccatcagca gcgcccagcc
      901 atcgaccagc acgaacagca acagcttgaa cggcagcgat acgatcgtcg ggctcatcat
      961 catcatgcca aggctcatca gcaccgatgc caccaccaga tcgatcacca gaaacggcag
     1021 gaacagcatg aagccgatct ggaacgcggt cttgagctcg gaggtgacga aggcgggaag
     1081 caggatcgag aacgggacgt cctggctgcg cgcaaagcgg ggcgccttgg ccatttcggc
     1141 gaacatcgcg aggtctttct cgcgggtctg gcggatcatg aaggcgtgaa attcgccgcc
     1201 ggcactggcg atggcctgtt cggcattgat cgtgccggcg gcataggggg cgattgcctg
     1261 ggcgttcacc cgttcgagcg tgggagccat gacaaacagt gaaaggaaca gcgagaggcc
     1321 gatcaacacc tggttgggcg ggctctgctg caggcccagc gcctgacgca ggatcgacag
     1381 caccacgaga atccgggtaa agctggtcat catcaagacc agtgcgggca gcatggtcag
     1441 aagccccatg atgaccagca gctgcagcga aagcgtcatg ccggggccct gcgaaccacc
     1501 catcgtgcta aaggcgcggt cgagcgcgcc ggggatggcg ctctgggcat gggccgcaac
     1561 cggcagcagc agggcgggaa acgcaagcag ccttctcatt gcccggcctc ccgcgcgggt
     1621 gcttcggcca gccgggtcag cccctggcgc gaggcggaga caaggatctc acggccatga
     1681 aattcgatca ccgccagttt cagcgttggc gatagcatgg tggtctcgat gatccgtacc
     1741 gaccgcgcgg ccgattggct gccggcgatc ctggcctgca ggcgctgtgc aagtttcagc
     1801 gtgccccaag ctgccgcacc gatcagcggc agcaggacga tcagcttgat cagataccag
     1861 atcatgctgc acctgcctgc gtttcggtat cggcgccggc gagttcgaca atccgcagtc
     1921 cgaaccgccc cccttgggcc acgatctcgc cgcgggcgat cagcttgccg ttgaccatca
     1981 cgtcgagcag ctcatcggtc agccgatcga gcatgatcac gctttcctcg cccagcgcca
     2041 gaacgtcgcg cagcttcatc tcggcgcggc ccagttcgac cgaaaggcgg acatcgactt
     2101 ccttgatcag gtcgaagccg cgggtgtgca atgtcatcgt cgtgttcctt caggcaatct
     2161 gggtcagctt gatcgcgatg cgatcgtcct gggcgccaac ggtgccgcgc gcgagcgttg
     2221 cggtccccag cgtgattggc acggcgcgcg ccaccgccac gggcagtacg gttccgggtt
     2281 caagcgcgga cagccgcgaa atcggcaccg ccatgtcgac cagcgtggcg accagggtca
     2341 gcggcatggt tgcaaacggt tcggccgccg gatcggcaga acgtggctcg cgtggcgtgg
     2401 cggcggcatt gccgaacagg cggggcaatg aaaccagcgg caaagcgatg gtgaacttcc
     2461 acggtgcgcg tgcccctgcg gccacttcgc acgaaagcag cgccagccgg gctccggccg
     2521 ggaacggagc cagttcggca aggctggtat cgcggcgcaa ggccttgagc ccatcctggc
     2581 ccatcgctcc acccagtgct gaaatcagca gcgcctcgat ccggtcgatc atcaggctgg
     2641 ccgaaagtgg aaagaccttg ggcataacgg ccggggcatc gcccttgccg ccaaaggccc
     2701 ggtcgaccag ccgcagcagg cccgaacctt caagcgaggc aagcagcgcg atgcccggaa
     2761 cgccgctcgc gatcaggctg ttggctgcca gcgggccaag ctcgctggcg agttcgcctt
     2821 cggacagttc gcgcgcggcg ctgacgtgga caaccggctc ctcgctgccc agcagccgtg
     2881 ccagctcggg tgcgagcagc cgggccagcc gatcccccat ccgggtgaaa tcgcccagca
     2941 ggtcggccgg ttcgcagccg gtcgcgcgtt gcaacaactc ggggcaatgc tgcgcagcga
     3001 tccgctcagc ggtaaaggcg cgttcgggct tcactggacg aggtagctct tgaagtagac
     3061 ctgatcgacc ccgccaaagc cctcggtctt gaccagcacc ttgttgatcg catcggtcat
     3121 gcgcttttgc aggcgctgct tgccatcggg ggtaaaggca tcctcctcgg gcgtgtcggc
     3181 cagcaccacc aggatctcgc tgcggatcgc cagttcgtgc tttttcagcc acatcagcac
     3241 ccggccatcg cgccgggtcg aacaggccag gctgatctgg acgagcgcgc tcgaattcct
     3301 gaggttcgag gtaaagtcct cggtgaagtt gtaatagctg gtgcggtatt cgctgccgcc
     3361 ctcgccctcg acttcggggg accttcacct tccttggctt cggtcttggg ggcataggga
     3421 tcttcctcgc ccttcttgac cagcttgggg tgatcggcct ccttcttttc ctcggcatgg
     3481 tcgccaagca tcccggcctg gaccagtgcg aaggtgccgc caccgccggc tgcgatcagg
     3541 atcaggccga tcaggccctt gatcaggaag cccttgccct tcttcttcct gggcttggcc
     3601 tcggcttcgt tactgtcact cattgttcgg taccctcgga ttggtctgtt ggtcaggcaa
     3661 agatcccgcc gcgcgggtta ccgcgttcgg gtgcgcggtg ttcgggcctg ggctgctggc
     3721 gtggctgatc gtcgcggcgt tcaggcgagc cgccgcgcga ttgcgccgtg cttcccccgg
     3781 ttccggcatt gccggttgcg ttctgctcgg gccgggcatt gccctggcca ggtgcttcgg
     3841 cggcaagggc ctgcagaact ggcggcgttg cggtgctggc gatccgggca aagtcgggat
     3901 cggcgcttgc cagcgaaatg ctcagcgcct cgccatcgtg gcgcaggcgc aggctgattt
     3961 cgccgaaatc ggcatgctga accgcgatcg agaccgcctg cggctgcacc gcatcgcgcg
     4021 cagccaccag ccggtcgatc aggccggcaa aatcatgcgg acgcggtgcg gcgtcggttt
     4081 gaaggctggg tc
//
LOCUS       DASUDV010000593         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_87484,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000593 DASUDV010000000
VERSION     DASUDV010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87484"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /gene="secF"
                     /locus_tag="VFV30_13135"
     CDS             <1..619
                     /gene="secF"
                     /locus_tag="VFV30_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX4849083.1"
                     /translation="GHADARIDGVDSVSGKVSGELFRTGMLALSLAMVAISIYIWIRF
                     EWQFGVGALFSLVHDVSLTLGMFALTQMEFDLNIVAAILTLIGYSLNDTIVVYDRIRE
                     NLKKFRRMPVPELLDLSVNETLSRTVVTSLSVLITLLALLLLGPNVIFGFTAAITLGI
                     FVGTYSSIYMAAPILIWLKVNSSSFVPTETELDKQERMARELGKA"
     gene            complement(633..1811)
                     /locus_tag="VFV30_13140"
     CDS             complement(633..1811)
                     /locus_tag="VFV30_13140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4849084.1"
                     /translation="MKPLRVLSLSTLYPNDQTPNFGVFVERQMQAVAVRGDVDLVMVN
                     PVGVPPFPLSLLPRYRQLARLPSSEDRGGVQVLRPRFRLGPGPLGRFNAAAEAGAVLP
                     LARQLHAEAPFDLVDAQFFWPDGPAALKVARELGLPVSIKARGADVHYWGEAAGIAAQ
                     VRGAARDADGLLAVSAGLADDIAAWGVPRDKITIHRTGLDRTVFKPLDKAQCRAALGL
                     PQDRPVLATVGALIARKGQRHAIEALAQLSGAVLVLAGSGPDLATLRAVAEAQGVEDR
                     VHFLGAVPHGDLPQVLSAADVVVLPIASEGLANAWVEALACGTPVVTTPIPGALELLT
                     DPAWGRTAPREAGAIAAAVRRVLTDGTPREVVQQGAAQFSWEANAAALVAHWRRLAGR
                     "
     gene            complement(1808..2380)
                     /locus_tag="VFV30_13145"
     CDS             complement(1808..2380)
                     /locus_tag="VFV30_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4849085.1"
                     /translation="MINRIRDIRRSKNLTLAEVAARCKPPTTAQTIGRLETGTRQLSL
                     GWMNRIAAALGVDPELLVRGSEPGQPKYVARLGEDGAEALAAPRDAILPTALGGDGVL
                     VAMMIEASAGQYRSGDQLWLRQHGPEDYARLLNRDVLVPRAGGRFAFGRMIDRDEHRV
                     AVLPPDPGQRQIVIDNPAWIAAAEMLVRAL"
     gene            2479..2949
                     /locus_tag="VFV30_13150"
     CDS             2479..2949
                     /locus_tag="VFV30_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX4849086.1"
                     /translation="MRQQLVERELTIEGPAARAPRRGRRSVTVNLAESPLAWLHARGH
                     LSDVQLAAGEVLRRDWEAAQLSPGVTMRWDPVRVQGGGERGLNASERQLAARARFDGA
                     VAAAGPGLSDVLWRVVCAGETLPDAEKALAWPVRSGKLVLKLALDRVAGFYRLG"
     gene            complement(2954..>3599)
                     /locus_tag="VFV30_13155"
     CDS             complement(2954..>3599)
                     /locus_tag="VFV30_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4849087.1"
                     /translation="HIQGYLKDFLFDPGLVDARVGTLSGGEQSRLLLAREFARKSNLL
                     VLDEPTNDLDLETLDLLQEVIADYDGTVLIVSHDRDFLDRTVTITLGLDGSGKVDVVA
                     GGYADWEKQRATRRIAPVKPAKPAVEVAPPPPPAPKKAKLSYKDQRDYDLLPGRIEEL
                     LAQIARDEAALADPALYTRDPARFAALTKAIEAARAEKDAAEERWLELAELVEG"
BASE COUNT          573 a         1212 c         1220 g          594 t
ORIGIN      
        1 cggccatgcc gatgcccgga tcgacggggt ggattcggtt tccggcaagg tttcgggcga
       61 actgttccgc accgggatgc tggcgctcag cctggcgatg gtggcgatct cgatctacat
      121 ctggatccgc ttcgaatggc agttcggggt gggggcgctg ttcagcctgg tccacgacgt
      181 ttcgttgacc cttggcatgt tcgcgctgac ccagatggaa ttcgatctca acatcgtcgc
      241 cgcgatcctg acgctgatcg gctattcgct caacgatacg atcgtggtct atgaccggat
      301 ccgcgaaaac ctgaagaagt tccgtcgtat gccggtgccc gaactgctcg atctgtcggt
      361 caacgaaacg ctcagccgca cggtggtgac ctcgctgtcg gtgctgatca cgctgcttgc
      421 gctgctgctg ctgggtccca acgtgatctt cggtttcacc gcggcgatca cgcttggcat
      481 cttcgttggc acctacagct cgatctacat ggctgcgccg atcctgatct ggctcaaggt
      541 caactcgtcg agcttcgtgc cgaccgagac cgagctggac aaacaggaac gcatggcccg
      601 cgaactgggt aaggcctgaa cgccatctgg ctctagcgcc cggccaatcg tcgccagtgc
      661 gcgacgaggg ccgcggcgtt ggcctcccag ctgaattggg cggcgccctg ctgcaccact
      721 tcgcgcggcg ttccgtccgt cagtaccctg cgcacggccg cggcgatggc ccctgcctcg
      781 cgcggagcgg tgcggcccca ggccggatcg gtcagcagtt ccagcgcgcc ggggatcggt
      841 gtggtgacca ccggagtgcc gcaggccagc gcctctaccc aggcattggc caggccctcg
      901 ctggcgatgg gcagcacgac gacatcggcg gctgaaagca cctgcggcag atcgccgtgc
      961 ggaacagctc ccaggaagtg cacgcggtct tccacgccct gggcttcggc cacggcgcgc
     1021 aaggttgcca gatcggggcc gcttccggca agtaccagca cggcccccga aagttgggcc
     1081 agggcctcga tcgcatggcg ctggcccttg cgggcgatca gcgcgccgac cgtggccagc
     1141 accggccgat cctgcggcag accaagcgcg gcgcggcatt gcgccttgtc gagcggtttg
     1201 aagaccgtgc gatcaagccc ggtgcggtgg attgtgattt tgtcccgcgg cacgccccag
     1261 gcggcgatat cgtcggccag accggccgat accgccagca agccatcggc gtcgcgcgcc
     1321 gcgccgcgaa cctgcgccgc gatcccggct gcctcacccc aatagtgaac gtcggcgccg
     1381 cgcgccttga ttgaaaccgg caggcccaat tcccgcgcca ctttcagcgc ggcgggacca
     1441 tcgggccaga agaactgggc gtcgaccaga tcgaacggcg cctcggcatg tagctgcctt
     1501 gcaagcggca ggaccgcgcc agcctctgcc gctgcgttga accgacccag cggacccgga
     1561 ccaagccgga accgcgggcg caggacctgc actccgccgc gatcctcgct ggaaggaagc
     1621 cttgccagct ggcgatagcg cggcaacagc gagagcggga agggcggcac gccgaccggg
     1681 ttgaccatca ccagatcgac atcgccgcgc acggccacgg cctgcatctg ccgttcgacg
     1741 aagaccccga aattgggggt ctgatcgttg ggatagagcg tcgaaaggga gagaacccgc
     1801 aagggcttca cagcgcgcgc accagcattt ccgcagcagc gatccaggcc gggttgtcga
     1861 tcacgatctg gcgctgcccc gggtcgggcg gaagcaccgc cacccggtgc tcgtcgcggt
     1921 cgatcatccg gccgaaggca aaacgccctc ccgcacgcgg caccagcaca tcgcggttga
     1981 gcagccgggc atagtcttcc gggccatgct ggcgcagcca cagctggtcg ccgctgcgat
     2041 actggcccgc gcttgcctcg atcatcatcg ccaccagcac gccatcgccg cccagcgccg
     2101 tgggcaggat cgcatcgcgc ggggcggcca gcgcctcggc cccgtcctcg cccagccggg
     2161 cgacatactt tggttggccc ggctcgctcc cgcgcaccag caattcggga tcgaccccca
     2221 gcgcggcggc gatccggttc atccagccaa gcgaaagctg cctggtgcca gtttcgagcc
     2281 ggccgatcgt ctgcgcggtg gtgggcggct tgcagcgcgc cgccacctcg gcaagggtca
     2341 ggttcttcga acggcggata tcgcggatgc ggttgatcat gctgggtctc gattaaccag
     2401 attggtgttt tcgctttcct acaacaccgc cgctttggca agccttggcg cgactcgaat
     2461 gcgcgaaagg gaacggcgat gcggcagcaa ctggtggaac gcgaactgac gatcgaaggt
     2521 ccggccgcgc gcgcgccgcg gcgcggcagg cgatcggtca cggtcaacct tgccgaaagc
     2581 ccgctggcct ggctgcatgc gcgtggccac ctgtccgacg tgcaactggc cgcgggcgaa
     2641 gtgctgcgcc gcgattggga ggctgcgcag ctatcccccg gggtcaccat gcgctgggat
     2701 ccggtgcggg tgcagggcgg gggcgagcgc ggcctgaacg ccagcgaacg ccagctggcc
     2761 gcgcgggccc gcttcgatgg agcggtggcg gctgccggac cgggcctcag cgatgtgttg
     2821 tggcgcgtgg tctgcgccgg cgaaacgctg cccgatgcgg aaaaggccct ggcctggccg
     2881 gtgcgcagcg gcaagctggt gctgaaactc gcgctcgatc gggtggcggg gttctatcgg
     2941 ctggggtgag ggcctaacct tcaaccagct ccgccagttc cagccagcgt tcctcggccg
     3001 cgtccttttc cgcgcgggcg gcctcgatcg ccttggtcag cgcggcgaac cgggccggat
     3061 cgcgggtgta gagcgccgga tcggccagtg cggcctcgtc acgggcgatc tgtgccagaa
     3121 gctcctcgat ccggcctggc agcagatcgt aatcgcgctg gtccttgtag gatagcttgg
     3181 ccttcttcgg cgcgggcggc gggggcgggg ccacttcgac cgctggcttg gccggtttga
     3241 ccggggcgat ccgccgcgtg gcccgctgct tttcccaatc ggcatagccg cccgcgacca
     3301 catcgacctt gcccgaaccg tcgagcccca gcgtgatcgt gaccgtgcga tcgaggaaat
     3361 cgcggtcgtg gctgacgatc agcaccgtgc catcataatc ggcgatcact tcctggagca
     3421 ggtcgagcgt ttcgagatca aggtcgttgg tcggctcgtc gagcaccagc aggttcgact
     3481 tgcgggcaaa ttcgcgcgcg agcagcaggc gcgactgttc gccgcccgaa agcgtgccaa
     3541 cccgggcatc gaccagcccg ggatcgaaca ggaagtcctt gagatagccc tggatgtgc
//
LOCUS       DASUDV010000594         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1324281,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000594 DASUDV010000000
VERSION     DASUDV010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324281"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFV30_13160"
     CDS             complement(<1..875)
                     /locus_tag="VFV30_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HEX4849088.1"
                     /translation="MLIVNSLIGIVLILAIAWLLSGDRKAIRLRVVGAAFALQAGLAA
                     LVLYVPFGKHMLKGASDGVSALLGYAHAGVEFLFGPLAKPEIGGTSFAIAALPVIIFF
                     AALISILYYLGIMQLVIKWIGGGLEKITGVSKVESLGAASNIFVGQSESPLVIRPYLA
                     ALTPSQLFCLMTVGMAGVAGTILAAYASMGIRIDYLVAAAFMSAPGGILMAKIIMPDP
                     KGEKIVEPDEVKVAGLEDEAPANIIMAASQGAQTGVKLAVAVGAMVLAFVALVALANG
                     ILGGIGGWFGQPDLS"
     gene            993..1640
                     /locus_tag="VFV30_13165"
     CDS             993..1640
                     /locus_tag="VFV30_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX4849089.1"
                     /translation="MQTVTMPRSLFVFSLLYGGLCVLAGVMGVKLASLGTWPVLGELA
                     VESGIFAFLFLVIISSAVAELFGQERANQLVRFGFVPLIVAMIMLTLVIRVVPPAPFW
                     GDQEAFAKLLGQGVRMQFAGLISYGTSQTLNVYLFSRIAGGQGKALVLRAWIASLLSQ
                     IVDTILFITISFYGQDMPLGQIMEGQIISKLVLSTIMVPPLIVVFVKLGRWLDRG"
     gene            1704..2600
                     /gene="argB"
                     /locus_tag="VFV30_13170"
     CDS             1704..2600
                     /gene="argB"
                     /locus_tag="VFV30_13170"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX4849090.1"
                     /translation="MTQSHDPQMLTKAETLVEALPYLQRYAGRTFVVKYGGHAMGDPE
                     LARDFAEDVVLLKAVGINPVVVHGGGPQIGAMLKKLGVESKFVDGLRVTDKATAEVAE
                     MVLSGAINKELVGWIAGAGGKALGISGKDGGLVTATKVERTTKDPESNIEQAIDLGFV
                     GEPTAVDVTLIETASAAGMIPVIAPIAPGVDGETYNINADTMAGAIAAALGAARLFLL
                     TDVAGVLDKQGNLLTDLTPADITKLQDDGTISGGMIPKLETCVHAVEAGCEAAVVLDG
                     RVPHAMLIEIFTSRGAGTLIRR"
     gene            2630..2923
                     /locus_tag="VFV30_13175"
     CDS             2630..2923
                     /locus_tag="VFV30_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX4849091.1"
                     /translation="MINEVLIPILVLFANVITTAVIVQFILGLLISFNVVNYHNQLVS
                     ALWTALNAILDPILAPIKRRMPATGGIDFSPIVLLLLIQVAIIILSYVGRQVG"
     gene            2920..3816
                     /gene="folD"
                     /locus_tag="VFV30_13180"
     CDS             2920..3816
                     /gene="folD"
                     /locus_tag="VFV30_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX4849092.1"
                     /translation="MSQAEVIDGKAFAAGLRERVGAAAAAFEAKAGRKAGLAVVLVGD
                     DPASQVYVRNKGKQTVEAGMASFEHKLPAETTQADLIALVEELNADPAVDGILVQLPL
                     PKHLDEQAVVERISPNKDVDGLTPTSTGRLALGLPGLVPCTPLGCLMLLKDKLGSLSG
                     KDAVVIGRSILVGKPMAQLLLAESCTVTIAHSRTQNLPDVVRRADIVVAAVGRPEMIK
                     GDWIKPGATVIDVGINRVPAAEEGKTRLVGDVDYAGASSVAGAITPVPGGVGPMTIAV
                     LLRNALVAAHRNAGVALAEGAL"
BASE COUNT          680 a         1231 c         1258 g          656 t
ORIGIN      
        1 aacgaaaggt caggctggcc gaaccagccg ccaatgccgc ccaggatacc attggcaaga
       61 gcgaccagcg caacgaaggc cagaaccatg gcgcccactg ccacggccag cttcaccccg
      121 gtctgcgctc cttgcgaagc cgccatgatg atgttggcag gagcctcatc ttcaaggccg
      181 gcaaccttga cttcatccgg ctcgacaatc ttttcgcctt taggatcggg catgatgatc
      241 ttggccatca ggatgccccc gggagccgac atgaaggcgg cggcgaccag gtaatcgatc
      301 cggatgccca tgctggcata ggccgcgagg atcgtgcccg cgacccccgc catgccgacg
      361 gtcatcaggc agaacagctg gctgggggtc agtgcggcaa ggtagggccg gatcaccagc
      421 gggctttccg actggccgac aaagatgttg ctggcggcgc ccaggctctc gaccttgctg
      481 accccggtga tcttctccag cccgccgccg atccacttga tcaccagctg catgatgccg
      541 aggtagtaga gaatcgagat cagcgccgcg aagaagatga ttaccggcag cgcggcaatg
      601 gcaaaactgg tgccgccaat ctccggcttg gccagcggtc cgaacaggaa ctcaaccccg
      661 gcgtgggcat agcccagcag cgcgctgacc ccgtccgaag cgcccttgag catatgcttg
      721 ccgaagggga catagagcac cagtgcggcc agcccggcct ggagtgcaaa cgccgcgcca
      781 acgacgcgca gcctgattgc cttgcggtca cccgacagga gccaggcaat cgccaggatc
      841 aatacgatcc cgatcaggct gttgacgatc agcatggcaa tggcgctccc ccagggtaat
      901 tccgcggcga gtggtaacgg gcaacgcgcg ccccgcctag ccgtatttgc cgggccttcc
      961 acaagcgcgg gaaggtcgct aagcctctgt cgatgcagac tgtcaccatg ccccgctcgc
     1021 tcttcgtctt ttcgctgctt tacggggggc tttgcgtcct ggccggggtg atgggggtca
     1081 agctggcctc gctcggtacc tggccggtgc tgggcgaact ggcggtggaa agcgggatct
     1141 tcgccttcct gttcctggtg atcatctcca gcgccgtggc cgaactgttc gggcaggaac
     1201 gggccaacca gctggtccgg ttcggcttcg tcccgctgat cgtggccatg atcatgctga
     1261 ccctggtgat ccgggtggtg ccgcccgcgc cgttctgggg cgatcaggaa gcctttgcca
     1321 agctgctcgg tcagggcgtg cggatgcagt ttgcggggct gatttcctat ggcacctcgc
     1381 agacgctcaa cgtctacctg ttcagccgga tcgccggggg gcaaggcaag gccctggtgc
     1441 tgcgggcttg gattgccagc ctgctgtcgc agatcgtcga cacgatcctg ttcatcacga
     1501 tcagcttcta tggccaggac atgccgctgg ggcagatcat ggaaggccag atcatctcga
     1561 agctggtgct ttcgacgatc atggtcccgc cgctgatcgt ggtcttcgtc aagctggggc
     1621 gctggcttga ccgcggctag cccacgcccg gcaagcgcga caaaacgctt caattccgcg
     1681 ccaagcgggc taaaggcgcg gggatgaccc agtcccacga tccccagatg ctcaccaagg
     1741 ccgaaacgct ggtcgaggca ctgccctatc tgcagcgcta tgccgggcgg accttcgtgg
     1801 tgaagtacgg cggccacgcg atgggcgatc ccgaactggc gcgcgatttt gccgaggacg
     1861 tggtgctgct gaaggcggtc gggatcaacc cggtcgtggt ccacggcggc ggcccgcaga
     1921 tcggcgccat gctcaagaag ctgggggtcg aaagcaagtt cgtcgacggc ctgcgggtga
     1981 ccgacaaggc cacggccgaa gtcgccgaaa tggtcctctc cggcgcgatc aacaaggagc
     2041 tggtcggctg gatcgccggg gcgggcggca aggcgctggg catttcgggc aaggacggcg
     2101 gcctcgtcac cgccaccaag gtcgagcgga ccaccaagga tcccgaaagc aacatcgagc
     2161 aggcgatcga cctgggcttc gtgggcgagc cgaccgcggt cgacgtgacg ctgatcgaaa
     2221 cggccagcgc cgccggcatg atcccggtga tcgcgccgat cgcccccggc gtcgatggcg
     2281 agacctacaa tatcaacgcc gataccatgg ccggggcaat tgccgccgcg ctgggcgcgg
     2341 cgcggctgtt cctgctgacc gatgtggccg gcgtgctcga caagcagggc aacctgctga
     2401 ccgacctgac cccggccgac attaccaagc tgcaggatga tggcacgatc agcggcggga
     2461 tgatccccaa gctcgaaacc tgcgtccacg cggtcgaggc cgggtgcgaa gctgcggtcg
     2521 tgcttgatgg ccgcgtcccg catgccatgc tgatcgaaat ctttacctcg cgcggggcag
     2581 gcaccctgat ccgccgctaa cggccctttc ttttccaagg accaacccga tgatcaacga
     2641 agtgctcatc ccgatactgg tgctgttcgc caatgtgatc acaaccgcgg tgatcgtgca
     2701 gttcatcctg gggctgctga tcagcttcaa cgtggtgaac tatcacaacc agctcgtttc
     2761 ggcgctgtgg actgcgctca atgcgatcct cgatccgatc ctggccccga tcaagcgccg
     2821 catgcccgcc accggcggga tcgatttctc gccgatcgtg ttgctgctgc tgatccaggt
     2881 ggcgatcatc atcctttcct acgtcgggcg ccaggtcgga tgagccaggc cgaggtaatc
     2941 gacgggaagg cattcgccgc cggcctgcgc gagcgggtcg gtgctgctgc cgctgcattt
     3001 gaagccaagg ccgggcggaa ggctggcctc gccgtggtgc tggtgggtga tgatccggcc
     3061 agccaggtct acgtccgcaa caagggcaag cagacggtcg aggccggcat ggcgagcttc
     3121 gagcacaagc tgccggccga aaccacccag gctgacctga ttgcgctggt tgaggagctc
     3181 aatgccgatc ccgctgtcga cggcatcctg gtccagctgc cgctccccaa gcatcttgac
     3241 gagcaggcgg tggttgagcg gatcagcccg aacaaggatg tcgacggcct gaccccgacc
     3301 agcaccggcc gcctggcgct ggggctgccg ggcctcgtgc cctgtacccc gcttggttgc
     3361 ctgatgctgc tcaaggacaa gctgggcagc ctttcgggca aggatgccgt ggtaatcggg
     3421 cgttcgatcc tggtcggcaa gccgatggcg cagctgctgc tggccgaaag ctgcactgtc
     3481 accatcgccc acagccgcac gcagaacctg cccgatgtgg tccgacgcgc cgatatcgtc
     3541 gtcgccgcgg tcggtcgacc ggagatgatc aagggtgact ggatcaagcc cggcgctacg
     3601 gtgatcgacg tgggcatcaa ccgggtcccc gcagccgagg aaggcaagac gcgcctggtc
     3661 ggcgatgtcg actatgccgg ggccagcagc gttgccgggg cgatcacgcc ggttccgggc
     3721 ggggtcggcc cgatgaccat cgccgtgctg ctgcgcaacg cactggtagc cgcacaccgc
     3781 aatgccggcg tggcgctcgc cgaaggtgcg ctttagcggg cgcgg
//
LOCUS       DASUDV010000595         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1589554,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000595 DASUDV010000000
VERSION     DASUDV010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589554"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFV30_13185"
     CDS             <1..565
                     /locus_tag="VFV30_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4849093.1"
                     /translation="VLLRMCSEVLGPGWSPRLVCFSHAAPPAADLPIFNRLFRSPLQF
                     DSELTAIIIDRADLDRPNPKADEQLARHARQLLDTVMNSGDRTVVQDVDQLIRLLMPA
                     GRASIQNCAASMGMTVRTLQRMLDGAGTSFSSLLNEARMQLATQYLANPQMRITDIAG
                     MLGYSSIGAFSRWHRSAFGAPARAMRP"
     gene            587..715
                     /locus_tag="VFV30_13190"
     CDS             587..715
                     /locus_tag="VFV30_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849094.1"
                     /translation="MAAALQQADPAAPFGWRIAYAPGIGHDSGGMAPFAVPYLVKP"
     gene            895..1407
                     /locus_tag="VFV30_13195"
     CDS             895..1407
                     /locus_tag="VFV30_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849095.1"
                     /translation="MKKLAALTFALTSCGPSDRLDWSCFSGVRTYSYANDAGELTAYT
                     VNFREERASAFGRVRSLHDDSVRSRDMEFDLDDQYFAFGNTVVAANLFSERSWTRGIL
                     QCSRIEQGPPANDAVARVVCRERGAKGSLEYLFDKTRGITEFQLNYADGSVATFRLKG
                     QSGLGETCPS"
     gene            complement(1459..1545)
                     /locus_tag="VFV30_13200"
     tRNA            complement(1459..1545)
                     /locus_tag="VFV30_13200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1509..1511),aa:Leu,seq:gag)
     gene            complement(1665..2603)
                     /locus_tag="VFV30_13205"
     CDS             complement(1665..2603)
                     /locus_tag="VFV30_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEX4849096.1"
                     /translation="MLNATTMIIACPACNTRYVVPDSAIGVDGRTVRCAKCRHSWFQE
                     GPVLDLAQPAAPVAEPAPPPPPVAAPEPAPEPAPPPVPEPVAPPIAAPQPEPEPQPEP
                     APAPVSTDVGVPPPAWDEPAEEEPAPPPPLSFAEDGVAPPPAAGLRRPPPEPDDAGSQ
                     FDYEPPFRPRRNPARMWTIAAALFAVVALGAVAATAWFGLPSWMPFAQPLFAEEQPGL
                     KLDFPEKRQAQRQLPDQTWFFEVNGTVTNISQVSRSVPPILAVLRDARGTVVYNAEIR
                     PPKRVLAPGESVSINEALVPVPKAAVRAEYGWKPGS"
     gene            2729..>3396
                     /gene="ftsE"
                     /locus_tag="VFV30_13210"
     CDS             2729..>3396
                     /gene="ftsE"
                     /locus_tag="VFV30_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX4849097.1"
                     /translation="MNSTAEGEIVQFDNVGLRYGTDREVLSDVSFTLFPGSFYFLTGA
                     SGAGKSSLLRLLYLAQRPSRGMIRMFGTDAITMPREHLPGLRRRLGVVFQDFRLVSHL
                     SAFDNVALPLRVAGVPERELLKPVADMLEWVKLGERMHALPAQLSGGEQQRVAIARAV
                     IGRPEMLIADEPTGNVDPEMAVKLLRLFEALNRMGTTVVVATHDLHLLKKVPDSLIMR
                     LDKGR"
BASE COUNT          549 a         1094 c         1173 g          580 t
ORIGIN      
        1 cgtcttgctg cggatgtgca gcgaagtgct gggcccgggc tggtcgccgc ggctggtgtg
       61 cttttcccat gccgcgccgc cggccgccga cctgccgatc ttcaaccgcc tgttccgcag
      121 cccgctgcag ttcgacagcg agctgaccgc gatcatcatc gaccgggccg atctcgaccg
      181 ccccaacccg aaggccgacg aacagctggc ccgccatgcc cggcaattgc tcgatacggt
      241 catgaactcg ggcgatcgca ccgtggttca ggatgtcgac cagctgatcc gcctgctgat
      301 gcccgccggc cgggcctcga tccagaactg cgccgcctcg atggggatga ccgtccgcac
      361 cctgcagcgg atgctggacg gtgccggcac cagtttcagc agcttgctga acgaagcgcg
      421 gatgcagctg gcaacccagt acctggccaa cccccagatg cggatcaccg atattgccgg
      481 aatgctcggc tacagttcga tcggcgcgtt cagccgctgg caccggtcgg cctttggcgc
      541 accggcacgg gccatgcggc cgtgacgccg gaacgcatca gaggctatgg ccgcagcgct
      601 ccagcaggcc gacccggccg cgccgttcgg ctggcggatc gcttatgccc ccggcatcgg
      661 ccacgacagc ggcggcatgg ctccgttcgc ggtgccctat ctggtgaaac cctgatcgcg
      721 ttgaggaaag ggcccgaccg tatgtttgcc cagtgagggg cctttacatc cggacccgcc
      781 aatcgcccca gcccgatcaa ccagattttt ccacccgata tcagaggcgt ggcgaaacgt
      841 ttcagtctga tctaggatgc tttctttggc gcagatatcg cttgaggctt tgccatgaag
      901 aagctcgctg ccctgacctt cgctctgacc tcgtgcggcc caagcgacag actggattgg
      961 agctgtttca gcggcgttcg aacctacagc tacgcgaacg acgctggcga actgaccgcg
     1021 tacacggtta attttcgcga agagagggcc tcagcgttcg gtcgggtacg cagtctgcat
     1081 gacgactccg ttcgcagccg ggatatggaa tttgacctcg acgatcagta ctttgctttt
     1141 ggaaacacgg tggttgccgc gaacctgttt tccgagagat cctggacacg gggaatcctt
     1201 cagtgcagcc ggatcgaaca gggcccgccg gccaacgatg ccgtcgcccg ggttgtctgc
     1261 cgggagcggg gggccaaagg gtcgcttgaa tatctgttcg acaagacccg cggaatcacc
     1321 gaatttcagt tgaactatgc ggacggcagc gttgcgacgt tccgtctcaa agggcagtcg
     1381 ggcttgggag agacctgccc gtcgtagcgg cggcacagtt cgcccgaggg cgagaacagg
     1441 ttaccttgtc tgactgattg gtgcggtcga gaagactcga acttccacgg gctttcgccc
     1501 acaacgacct caacgttgcg cgtctaccaa ttccgccacg accgctaaat catcggagcg
     1561 cgcaatcact gggattcggg gcgccccggg ggggtaggag cgcgccctta gcaaaggggg
     1621 cagggggctg caagggcgga aaagccttgg cgccggggcg cggctcagct tccgggcttc
     1681 cagccgtatt cggcccgcac cgcggccttg ggcaccggca ccagcgcttc gttgatcgag
     1741 acactttcac ccggggccag cacgcgcttg ggcgggcgga tttcggcgtt gtagaccacc
     1801 gtgccgcggg catcgcgcag caccgccagg atcggcggga ccgagcgcga gacctgcgag
     1861 atgttggtga ccgtgccgtt gacctcgaaa aaccaggtct ggtccggcaa ctggcgctgg
     1921 gcctggcgct tttccgggaa gtccagcttc aggcccggct gctcttcggc gaacagcggc
     1981 tgggcgaagg gcatccagct gggcaggccg aaccaggccg tggccgcaac cgcgcccagc
     2041 gccaccactg cgaacagcgc ggcggcgatg gtccacatcc tggccgggtt gcggcgcggg
     2101 cggaacggcg gttcgtaatc gaactgcgag ccggcatcgt ccggctcggg cggggggcgc
     2161 cgcagaccgg cggctggcgg gggcgctacg ccgtcttccg caaagctcag cggcgggggc
     2221 ggggcgggct cctcttcggc gggttcgtcc caggcgggcg gcggcacgcc aacatcggtg
     2281 gaaaccggcg cgggggcggg ttcaggctgc ggttcaggct ccggctgcgg agcggcgatg
     2341 ggcggcgcta ccggctcggg cacaggcggc ggcgcgggtt ccggtgcagg ttccggcgcg
     2401 gccacgggcg gcgggggcgg ggccggttcg gccaccggcg cggctggctg ggccagatcc
     2461 agcaccgggc cttcctggaa ccaggaatgg cggcacttgg cgcagcggac ggtgcgcccg
     2521 tcgacgccga tcgcgctgtc gggaacgacg tagcgcgtgt tgcaggcggg acaggcgatg
     2581 atcatggtgg ttgcgttaag catgatgaac gcccatgcgg caagcactcg ccgtggaaag
     2641 gggggtggca atgccctttt ttccacatgg attggccgct atagcccggc gcgcacccat
     2701 tcaccgtcct ggacccaccc ctgatcccat gaacagcacc gccgaagggg aaattgtcca
     2761 gttcgacaat gtcgggcttc gctatggcac cgatcgcgag gtgctgagcg atgtgtcgtt
     2821 caccctgttc ccgggcagct tctatttcct gaccggcgcc agcggcgcgg gcaagtcgtc
     2881 gctgctgcgc ctgttgtacc tggcccagcg cccttcgcgc gggatgatcc ggatgttcgg
     2941 gaccgacgcg atcaccatgc cgcgcgagca tctgccgggc ctgcgccgcc ggctgggcgt
     3001 ggtgttccag gatttccggc tggtctcgca cctttcggcg ttcgacaacg tggccctgcc
     3061 gctgcgcgtc gccggcgtgc ccgagcgcga actgctcaag ccggtggccg acatgctgga
     3121 atgggtcaag ctgggtgagc ggatgcacgc cctgccggcc cagctttcgg gcggggaaca
     3181 gcagcgcgtg gcgattgccc gcgcggtgat cggccgtccg gaaatgctga tcgcggacga
     3241 gccgaccggc aacgtcgatc cggaaatggc ggtcaagctg ctgcgcctgt tcgaagcgct
     3301 gaaccggatg gggaccaccg tggtggtcgc cacccacgac ctgcacctgc tcaagaaggt
     3361 gccggattcg ctgatcatgc ggctcgacaa ggggcg
//
LOCUS       DASUDV010000596         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_926683,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000596 DASUDV010000000
VERSION     DASUDV010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926683"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFV30_13215"
     CDS             complement(<1..433)
                     /locus_tag="VFV30_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX4849098.1"
                     /translation="MDATHADMNEMLITLTSDIVAAHVSNNNVAVEEVAGLIGNVYTA
                     LAGLGHAPVAEEKLPEPAVSVRASVKPDYIVCLEDGKKLKMLKRHLMTHYNLTPDQYR
                     ARWNLPADYPMVAPNYAEKRRDLAKKIGLGRKPGQKRGRKPK"
     gene            complement(607..1074)
                     /locus_tag="VFV30_13220"
     CDS             complement(607..1074)
                     /locus_tag="VFV30_13220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4849099.1"
                     /translation="MIAPGDDIDRLMAVMRAAFDPAYGEAWTRRQVEDALLTGNCHYA
                     LIAPGGEPPRADEAAAGFFLSRHGYEDEELLLLAVDPAQRGKGLGGALIDWLARDAHA
                     RGAQRLFLEMRRGNPAEHLYRAKGFVPIGERLNYYRTPSGERLDAVTFCREME"
     gene            complement(1071..1688)
                     /gene="tsaB"
                     /locus_tag="VFV30_13225"
     CDS             complement(1071..1688)
                     /gene="tsaB"
                     /locus_tag="VFV30_13225"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX4849100.1"
                     /translation="MKTLVIDSATEACSVALFDGDALIAGECRVLGRGHAEQLVPMIE
                     ALPGKGRADRIAVALGPGSFTGVRVGLAAARALAFAWQADLVGYPTLALVAALAGRDH
                     PGAEVTVCMTGGHGEWFVQDFDSAGAPVAPLASLTPAAAAARGGAALVAGSQAEALVA
                     ARGSGTALTLWPDAAGFGLLDPARLSADTTPLYGRAPDARLPGAA"
     gene            complement(1707..2300)
                     /locus_tag="VFV30_13230"
     CDS             complement(1707..2300)
                     /locus_tag="VFV30_13230"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX4849101.1"
                     /translation="MSLPLPDASLDQLFRTARTYNGYLDKPVSTEQLHAIWDLMKMGP
                     TSANQLPARLVWVTTDEAKAKLAACTMPANGEKILKAPVSVIIAMDLQFHEQLPWLFP
                     HTDAKAWFTGNDALREKSAFLNTTLQGAYFIMAARALGLDTGPMTGFSNEAVDAAFFA
                     DQPEYKSVMLSTLGYGDPASIFDRSPRPDFDTFNRMA"
     gene            complement(2340..2915)
                     /locus_tag="VFV30_13235"
     CDS             complement(2340..2915)
                     /locus_tag="VFV30_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX4849102.1"
                     /translation="MFIETETTPNPATLKFLPGQEVMYAGTRDFRDEEQAAASPLATA
                     LFDLGDVTGVLFGRDFVAVTAAPGVDWSALKPQVVAILLDHFITQAPLFHAPSAGGIV
                     VPPEDEDFGDDPADAEIVEQIKELIETRVRPAVANDGGDIVYRGFRGGVVYLAMQGAC
                     SGCPSSTATLKNGIETLLKHYVPEVSEVRAA"
BASE COUNT          480 a          961 c         1041 g          505 t
ORIGIN      
        1 ccttcggctt gcggccgcgc ttctggccgg gcttgcggcc caggccgatc ttcttggcca
       61 ggtcgcggcg cttttcggcg tagttggggg caaccatcgg atagtcggcg ggcaggttcc
      121 agcgcgcgcg gtactgatcc ggggtcaggt tgtagtgcgt catcaggtga cgcttcagca
      181 tcttgagctt cttgccgtct tccaggcaga cgatgtagtc gggcttcacc gaagcgcgga
      241 cggaaacggc aggctcaggc agcttttctt cggcaacggg cgcgtggccc aggccggcaa
      301 gggcggtgta gacgttgccg atcaggccgg cgacttcttc cactgcgacg ttgttgttgc
      361 tgacgtgtgc ggcgacgatg tccgacgtca gcgtaatgag catttcgttc atgtcggcat
      421 gggttgcatc catggccttt tccttccgta cttggttgtc ccgagagttt tcctgttatc
      481 ggccgcacac acggtgtccg gcactttgcc ggttaggcct gtctccttaa caagacaaga
      541 tactgacagc ctttctcttc agtaaatttt gcgccgatcc ggagtaatcg cgccgaagct
      601 taaggcttat tccatttccc gacagaatgt tacggcatcc agtctttcgc cgcttggtgt
      661 gcggtaatag ttaaggcgtt ccccgatcgg aacgaagccc ttggcccggt aaagatgctc
      721 ggccgggttg ccgcggcgca tttccaggaa caaccgctgc gccccgcggg cgtgcgcatc
      781 gcgggccagc cagtcgatta gcgcgccgcc caggcccttg ccgcgctgcg cgggatcgac
      841 cgcaagtaaa agcagttcct cgtcttcgta accgtggcgc gaaaggaaga agccggccgc
      901 cgcttcgtcc gcgcgcgggg gttctccccc cggggcgatc agggcatagt ggcaattgcc
      961 ggtcagcagc gcgtcctcga cctggcggcg ggtccaggcc tcgccatagg ccggatcgaa
     1021 ggcggcgcgc atcaccgcca tcagccggtc gatatcgtcg ccgggcgcga tcatgccgcc
     1081 ccgggcaacc gcgcgtccgg cgcgcggccg taaagcgggg tggtgtcggc cgaaagccgg
     1141 gccggatcca gcaggccaaa gccggcggca tcgggccaca gcgtcagcgc cgtgccgctg
     1201 ccgcgcgccg caaccagcgc ttcggcttgg ctgccggcga ccagcgcggc cccgccgcgc
     1261 gccgctgccg ccgcaggggt gagcgaggcg aggggggcga ccggcgcacc ggcggaatcg
     1321 aaatcctgca cgaaccattc gccgtggccg ccggtcatgc agacggtcac ctccgcgccg
     1381 ggatggtccc gcccggccag cgccgcgacc agggccaggg tgggatagcc gaccagatcg
     1441 gcctgccagg caaaggccag cgcgcgggcc gcggccaggc ccacgcgcac gccggtaaag
     1501 ctgcccgggc ccagcgccac cgcgatccgg tccgcccggc ccttgccggg cagggcctcg
     1561 atcatcggca ccagctgttc ggcgtggccg cggcccagca cgcggcattc cccggcgatc
     1621 agcgcgtcgc cgtcaaacag ggcgaccgag caggcctcgg tcgcgctatc gatcaccagg
     1681 gttttcatag ggaagcgtct agcgcttcac gccatgcgat tgaaagtatc gaaatcgggc
     1741 cgcgggctgc ggtcgaaaat gctcgcggga tcgccgtagc ccagggtcga gagcatgacc
     1801 gacttgtatt ccggctgatc ggcaaagaag gcggcgtcga ccgcctcgtt ggaaaagccg
     1861 gtcatcggcc cggtatccag ccccagcgcg cgggcggcca tgatgaaata ggccccctgc
     1921 agcgtggtgt tgaggaaagc gctcttctcg cgcagggcat cgttgccggt aaaccaggcc
     1981 ttggcatcgg tgtgcgggaa cagccagggc agctgttcat ggaactgcag gtccatcgca
     2041 atgatcaccg agaccggggc cttcaggatc ttctcgccgt tggccggcat ggtgcaggcc
     2101 gccagcttgg ccttggcctc gtccgtggtc acccagacca gccgcgcggg cagctggttg
     2161 gcgctggtcg gacccatctt catcaggtcc cagatggcgt ggagctgctc ggtgctgacg
     2221 ggcttgtcga gatagccgtt gtaggtgcgg gcggtgcgga acagctgatc gagcgacgca
     2281 tcgggaagcg gcagggacat ggacactccg gcattgggaa agggaagaag cggggccgct
     2341 caggcggcgc gcacttcgct cacttcgggg acatagtgct tgagcagcgt ctcgatcccg
     2401 ttcttgagcg tggcggtgga actggggcag cccgagcagg cgccttgcat ggcaaggtag
     2461 accacgccgc cgcggaagcc gcggtagacg atgtcgccgc cgtcgttggc cacggccgga
     2521 cggacgcggg tttcgatcag ttccttgatc tgctccacga tctcggcatc ggccggatcg
     2581 tcgccgaaat cctcgtcctc gggcggaacc acgatcccgc cggcgctggg cgcgtggaac
     2641 agcggggcct gggtgatgaa gtggtcgagc aggatcgcca cgacctgcgg cttcagcgcc
     2701 gaccagtcga cccccggggc cgccgtcacc gccacgaaat cgcggccgaa cagcacgccg
     2761 gtcacgtcgc ccagatcgaa cagcgccgtg gccagcgggc tggctgcggc ctgctcctca
     2821 tcccggaaat cgcgggtgcc ggcgtacatc acctcctgcc cgggcaggaa cttcagcgtg
     2881 gcggggttgg gcgtcgtttc ggtttcgatg aacatgggcg ggatgtaggg accgggacgg
     2941 ggcggggcaa ggggccaaag gggcaggcga gccgggtgat tgcaacc
//
LOCUS       DASUDV010000597         5371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1523830,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000597 DASUDV010000000
VERSION     DASUDV010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523830"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VFV30_13240"
     CDS             complement(<1..1097)
                     /locus_tag="VFV30_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4849103.1"
                     /translation="MEELDPRPARVPTRTVLISIAGIWLCYLALITLRSLLLDRTYFA
                     EMLGLRSVVTLAGMAVTALAWMILRLFDNARIGLRMGLALVVMLPAALGLAMINRQVF
                     SGLDQRILGEQRNPTEVQIRHDTAGNVLVDVPDPPQLTPEQLADLQKKFAEQGLWRQL
                     TDIAIGRYFLLLAWAALYFAMVAAEQARAAERREGEYRRAAKAAELRSLRYQVNPHFL
                     FNTLNSLSALVMTGKGQAAEKMIQSLSNFYRRSLAEDPTGDIALDQEVALQRAYLDIE
                     AARFPDRLITRFDLPEALEQANVPGLILQPLVENAVKYAVAPTRRPVTVTIAAREEYG
                     RLVLEVSDDGPGAPKGGRAGFGIGLANVRDRL"
     gene            complement(1179..2048)
                     /locus_tag="VFV30_13245"
     CDS             complement(1179..2048)
                     /locus_tag="VFV30_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849104.1"
                     /translation="MNRRVHMAAAAGAVIATLAFLRDWLAAGTPVLIAMALVVAAGVA
                     YAVWNAFELVALFKASPSPAAPVRAAIPTPALAGARAASVRRIVDALAAEGVFAPDPP
                     DPRSLHGPIAERDETPNQEVVLTALLEADSYDQAFDLSRHFGNLAFHDSKAEQFEETI
                     AAQIADLARLTGPHLRIETVAIDLAIGGAAVAQSRCSIALSANGEPLTVQYRPAAKYL
                     STVLHVAVARRLRESGTDRRLAWLWNDQGVWLAALGEGAAQRLNTGPGKVRGGFGGWE
                     WVDETEPFAAGDA"
     gene            complement(2045..3559)
                     /locus_tag="VFV30_13250"
     CDS             complement(2045..3559)
                     /locus_tag="VFV30_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX4849105.1"
                     /translation="MVTIREEDLIESVADALQFISYFHPMDYIRALGVAYEAEQGPAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVNVFVEWGQDCRLESRRSLQDVVDEGVRRAY
                     LHPENKLRASVLADPAFTRRNTRDNTPCVLHVTMVPGNTVSIDVAAKGGGSENKSKFK
                     MMNPSDNIVDWVIEMLPQMGAGWCPPGMLGIGIGGTAEHCMLLAKQALMEPIDMAQLK
                     QRGAQTDIEAMRIEIFDKVNALGIGAQGLGGLSTILDVKILDAPCHAAGKPVAMIPNC
                     AATRHAHFTLDGSGPTYLEKPNLAEWPDVHWTPDKAAKRVNLDTLTPEEVRGWKQGDR
                     LLLNGKMLTGRDAAHKRIQDMLAKGEALPVDFKGRAIYYVGPVDPVMGEVVGPAGPTT
                     ATRMDKFTEMMCELGLLVMIGKAERGQGATEVIAKSKTAYLMAVGGAAYLVSRAIREA
                     KVVAFEDLGMEAIYEFTVEDMPVTVAVDSEGNNVHQLAPLVWKDRIAKEGLLTP"
     gene            3727..4335
                     /locus_tag="VFV30_13255"
     CDS             3727..4335
                     /locus_tag="VFV30_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX4849106.1"
                     /translation="MTLAAESIASQLEDLSAARRAMIDSQLRVSGINQDWLLAALARV
                     PREEFVPVAARGHAYIDRAIPLGEGRMLPAPLVQAMLLAEAAPEAGDKVLVVSPAGYL
                     AALVEPLVQSVDTVEAGAALALQAKGAFTLVLIDGAIEVLPPALVDALAEGGRVVCGR
                     VERGVTRITAGHKNGGKVELTARADVGMPVLAEFAAPKTWSF"
     gene            4340..>5371
                     /locus_tag="VFV30_13260"
     CDS             4340..>5371
                     /locus_tag="VFV30_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4849107.1"
                     /translation="MARRSEAGFIRTKIARYALMAGAAAMVTTPVLADELRDALVMAY
                     NTNPTLQAARATLRATDEGVPIARSQGLPSLSGTATYSEFVDGSSGNPLAPDRNLDAQ
                     LNFGVPIYAGGGIRNSIRSAETRVIAGRADLRAAETAVFSQVVAVYMDVILAESVVEL
                     NRANVKRLEVNLQATRDRFEIGDLTRTDVAQSESRLAFAQGQARTAEANLISARERYI
                     QVVGKAPENLKTPPPLVGLPGSADDAVAIALESNPDLLAARQRSRAAGYDSAAAGSGR
                     MPTVSVFANTGYNDSLGTNQIPGAVQSTTSTTVGVRATVPLFQGGLPSAQRRQAQARE
                     GALLEQEIGI"
BASE COUNT          854 a         1832 c         1819 g          866 t
ORIGIN      
        1 agccggtcgc ggacattggc aagcccgatc ccgaacccgg ccctgcctcc ctttggcgca
       61 ccggggccgt cgtcgctgac ctccagcacc agccggccgt actcctcgcg cgcggcgatc
      121 gtgacggtca ccgggcggcg ggtgggcgcg acggcgtatt tcaccgcgtt ctcgaccagt
      181 ggctgcagga tcaacccggg cacgttggcc tgttccagcg cttcgggcag atcgaaccgg
      241 gtgatcagcc ggtcgggaaa gcgcgccgcc tcgatgtcca gataggcgcg ctgcagcgcc
      301 acttcctggt cgagcgcgat atcgccggtc ggatcctcgg ccaggctgcg gcggtagaag
      361 ttggacagcg actggatcat tttctccgcc gcctggccct ttccagtcat gaccagcgcc
      421 gacagcgaat tgagcgtgtt gaacaggaag tgcgggttga cctgatagcg cagcgaacgc
      481 agctcggccg ccttggccgc gcggcggtat tcgccctcgc gccgctcggc ggcccgggcc
      541 tgttcggccg cgaccatcgc gaaatagagc gccgcccagg ccagcagcag gaaatagcgg
      601 ccgatcgcga tgtcggtcag ctggcgccac agcccctgct cggcaaactt cttctgcagg
      661 tcggccagct gttcgggggt cagctgcggc ggatcgggca catcgaccag gacgttgccg
      721 gccgtgtcgt ggcggatctg cacttcggtg gggttgcgct gctcgcccag gatccgctgg
      781 tcgagaccgg agaagacctg gcggttgatc atcgccaggc ccagcgcggc gggcagcatg
      841 accaccagcg ccaggcccat ccgcagcccg atccgggcgt tgtcgaacag ccgcaggatc
      901 atccaggcca gggcggtaac cgccatcccg gccagggtga cgaccgagcg caggcccagc
      961 atctcggcga agtaggtccg gtcgagcagg aggctgcgca gggtgatgag cgccagatag
     1021 cacagccaga tccccgcaat cgagatcagc acggtccgcg tcggcacgcg ggcggggcgg
     1081 gggtccagct cctccatcag accggcagcg atagcccgtc cggggcccgg agcgccagcg
     1141 gcttcgtcga cgcgacttgg gccttggtcg ataggtcgct aggcatcccc ggcggcgaaa
     1201 ggctcggtct catcgaccca ctcccagccg ccaaatccgc cgcgaacctt gccgggaccg
     1261 gtattgagcc gctgtgccgc gccttcgccg agcgcggcaa gccagacgcc ctgatcgttc
     1321 cacagccagg caagccggcg gtccgtgccg gattcccgca gcctgcgggc aacggcaaca
     1381 tgcagcacgg ttgaaaggta tttcgccgcg gggcggtatt gcactgtcag tggctcgcca
     1441 ttggcggaca acgcaatcga acaacgggac tgcgcaacgg cggctccacc gatggccaga
     1501 tcgatcgcga cggtctcgat ccgcaaatgc ggcccggtca ggcgcgccag atcggcgatt
     1561 tgcgcggcga tcgtttcctc gaactgctcg gccttgctgt catgaaaggc aaggttgccg
     1621 aaatgacggg acagatcgaa ggcctgatca tagctgtccg cttcgagcag cgcggtcagc
     1681 accacttcct ggttcggggt ctcgtcacgc tctgcgatcg ggccatggag cgagcggggg
     1741 tcgggcggat ccggtgcgaa cacgccctcg gcggcaaggg catcgacaat ccgcctgacg
     1801 ctggccgcgc gggcgccggc caaggcgggc gtcgggattg cggcgcgcac cggggcggcc
     1861 ggactgggcg aggctttgaa caaggccacc agttcgaacg cgttccagac cgcgtaggcc
     1921 acgccggcag cgacgaccag ggccatggca atcagaacgg gcgtgcctgc cgccagccag
     1981 tccctcagga aggccaaggt ggcgatcaca gcgcccgcag ccgcggccat gtgaacgcgg
     2041 cgattcacgg cgtaagcaga ccttctttgg cgatccggtc cttccagacc agcggggcga
     2101 gctggtggac attgttgccc tcgctgtcga ccgcgacggt caccggcatg tcctcgaccg
     2161 tgaactcgta gatcgcctcc atgccgaggt cttcgaacgc cacgaccttc gcctcgcgga
     2221 tcgcgcggct gaccagatag gccgcgccgc cgaccgccat cagataggcg gtcttgctct
     2281 tggcgatcac ttcggtcgcg ccctggccgc gttcggcctt gccgatcatc accagcaggc
     2341 ccagctcgca catcatctcg gtgaacttgt ccatccgggt ggcggtggtc gggccggccg
     2401 ggccgaccac ttcgcccatc accggatcga ccgggccgac gtaatagatc gcgcggccct
     2461 tgaaatcgac cggcagggcc tcgcccttgg ccagcatgtc ctggatccgc ttgtgcgcgg
     2521 catcgcgccc ggtcagcatc ttgccgttga gcagcagccg gtcgccctgc ttccagccgc
     2581 gcacctcttc gggggtcagc gtgtcgaggt tgacgcgctt ggcggccttg tccggagtcc
     2641 agtggacatc gggccattcg gccaggttgg gcttttccag ataggtcggg cccgatccgt
     2701 cgagcgtgaa atgggcgtgg cgggttgccg cgcagttggg gatcatcgcc accggcttgc
     2761 ccgcggcgtg gcacggcgcg tcgaggatct tgacgtcgag gatcgtcgaa agcccgccca
     2821 ggccctgggc gccgatgccc agcgcgttga ccttgtcgaa aatctcgatc cgcatcgcct
     2881 cgatgtcggt ctgggccccg cgctgcttca gctgggccat gtcgatcggc tccatcagcg
     2941 cctgcttggc cagcagcatg cagtgttccg ccgtgccgcc gatgccgatc cccagcatcc
     3001 ccggcgggca ccagccggcg cccatctggg gcagcatctc gatcacccag tcgacgatgt
     3061 tgtcgctggg gttcatcatc ttgaacttcg acttgttctc gctcccgccg cccttggccg
     3121 ccacgtcgat cgaaacggtg tttccgggca ccatggtgac gtgaagcacg caaggggtgt
     3181 tgtcgcgggt gttgcggcgg gtgaaggcgg gatcggccag caccgaggcg cgcagcttgt
     3241 tttccgggtg gagataggcg cggcgcaccc cttcatcgac cacgtcctgc agcgaccggc
     3301 gcgattccag ccggcaatcc tggccccatt cgacgaagac gttgacgatc ccggtgtcct
     3361 ggcagatcgg acggtgccct tcggcgcaca tccggctgtt ggtcaggatc tgggcgatgg
     3421 catccttggc cgcggggccc tgctcggcct cataggccac gccgagcgcg cggatgtagt
     3481 ccatcgggtg aaaatagctg ataaattgca gcgcatcggc cacgctttcg atcaggtctt
     3541 cctcgcggat cgtcaccagg gtcacgccat cacctctttc gccttggggc ttgtcgttgc
     3601 cgcccccata ggcccgcaaa ccgcccgccg tcaaagcgct tggcgcgaaa cggcggttcg
     3661 cttaagggga gagggatttg catcgctgtg ttattcatgc aatacagcac ctcagaggcc
     3721 cgatccatga cccttgccgc cgaatccatt gcttcccagc ttgaagacct gagcgccgcc
     3781 cgccgcgcga tgatcgacag ccagttgcgc gtgtcgggga tcaaccagga ctggctgctg
     3841 gccgcgctgg cccgcgtccc gcgcgaagag ttcgtgccgg tggccgcccg cggccatgcc
     3901 tatatcgacc gcgcgatccc gctgggcgaa gggcgcatgc tgccggctcc gctggtccag
     3961 gccatgctgc tggccgaagc cgcgcccgag gctggcgaca aggtgctggt ggtcagcccc
     4021 gccggctatc tggcggcgct ggtcgaaccg ctggtgcagt cggtcgatac ggtggaggca
     4081 ggcgccgccc ttgcccttca agcgaagggc gctttcacgc tggtgctgat cgacggcgcg
     4141 atcgaagtgc tgcccccggc cctggtcgat gcgctggcag agggcggccg ggtggtctgc
     4201 ggccgggttg aacgcggcgt gacccggatc acggcgggcc acaagaacgg cggtaaggtc
     4261 gaactgacgg cccgggccga tgtaggaatg ccggtgctgg cggaattcgc ggcaccgaag
     4321 acttggagtt tctgacggta tggcacgtcg cagcgaagca ggtttcatca ggacgaagat
     4381 cgcgcgttac gcgctgatgg ccggcgccgc ggcgatggtg accactccgg ttctggccga
     4441 cgaactgcgc gacgcgctgg tcatggccta caacaccaac cccaccctgc aggccgcccg
     4501 ggcaaccctg cgcgcgaccg acgagggcgt gccgatcgcc cgctcgcagg ggctgccctc
     4561 gctttcgggc acggcgacct acagcgaatt cgtcgacggc agcagcggca acccgctggc
     4621 gccggaccga aacctcgatg cccagctcaa tttcggcgtg ccgatctacg ccgggggcgg
     4681 gatccgcaat tcgatccgct cggccgaaac ccgggtgatc gccggccggg ccgatctgcg
     4741 cgcggccgag accgcagtct tcagccaggt cgtggcggtc tacatggacg tgatcctggc
     4801 cgaatcggtg gtcgaactga accgcgccaa cgtgaagcgg ctggaagtga accttcaggc
     4861 cacccgcgac cggttcgaga tcggcgacct gacccgcacc gacgtggccc agtccgaaag
     4921 ccggctggcc tttgcccagg gccaggcccg caccgccgaa gccaacctga tctcggcccg
     4981 cgaacgctat atccaggtgg tcggcaaggc gcccgagaac ctgaagaccc cgccgccgct
     5041 ggtgggcctg ccgggaagcg ccgacgatgc cgtggcgatc gcgctggaat cgaaccccga
     5101 tctgctcgcc gcgcgccagc gcagccgcgc tgccggctat gacagcgcgg cggccggttc
     5161 gggccggatg ccgacggtct cggtctttgc caacaccggc tacaacgatt cgctgggcac
     5221 caaccagatc cccggcgcgg tgcagagcac gaccagcacc acggtgggcg tccgcgccac
     5281 ggtgccgctg ttccagggcg gcctgccgtc ggcccagcgc cgccaggccc aggcccgcga
     5341 aggcgcgctg ctcgagcagg aaatcgggat c
//
LOCUS       DASUDV010000598         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_529360,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000598 DASUDV010000000
VERSION     DASUDV010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529360"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1489
                     /locus_tag="VFV30_13265"
     CDS             209..1489
                     /locus_tag="VFV30_13265"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX4849108.1"
                     /translation="MRIMVLGAGGFIGRAIVAELIGHGHQVVAVQRPGRSADALPPGA
                     EALALDLSRATSPADWREAVRGVDMVVNAAGILRGAQMEAVHVAMPVALHQAASEAGV
                     RRVVLISAISARPDVPTDYAQSKLRGEAALRQCGLGWTILRPSLVYGEGSYGGTSLLR
                     GLAALPWRVPLPGQGQFAFSPIHVRDLARAVREVCEDERHAGQVLDPCGPETLSLSDL
                     LGRYRRWLGFGEARFVAVPMPVMRLLGRIGDRLGDGPVSTNSLVQLVAGNAGDGEGFA
                     RTIGFAPRSLGAALRESPAQVQDRWHARLFFLAPAIRWTLAMLWLVSALLGLGWGAEQ
                     ARAVVAGLGLPQGMADPLRWGGSALDLVIAATVLADRRGRIATGLQLLAVVGYSLVIG
                     LAIPALWLDPLGPLLKNLPILVLILVHGAIAEQR"
     gene            1489..1962
                     /locus_tag="VFV30_13270"
     CDS             1489..1962
                     /locus_tag="VFV30_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEX4849109.1"
                     /translation="MEPFLLVKWLHILSSTVLFGFGAGTAWYMWNAHLGGDPRTIAGV
                     ARMVVRADWIFTGTSGIVQPASGLVLAHMAGWPLDTPWLVASYALYLLAFVCWVPVVG
                     LQIRARDLAAHAVASGKPLGEDYRRAMRWWFALGWPAFLGLLGVFWLMVAKPALW"
BASE COUNT          255 a          667 c          725 g          359 t
ORIGIN      
        1 accagcgcgc cgcccatgcc gaggaacgcg gtcatccacc agcccatcgc gaccgggccg
       61 ggatagaacc agcgggccag cgccccgacg aacaacccgc tgatcagcgc gccgatgatg
      121 ttgagcatgg ggcatttctc cactgcattg gaacaggtgt cgctgcgcct gattgccagc
      181 acagcccccg atctggcaag caggcatgat gcggatcatg gtgcttggcg cgggcggctt
      241 tatcgggcgg gcaatcgttg ccgaactgat cggccatggc caccaggtgg tggcggtgca
      301 gcgtcccggt cggtcggccg atgccctacc gccgggcgcc gaagcactgg cgctcgatct
      361 gtcgcgggcc acctcgcctg ctgattggcg cgaggccgtg cgcggggtcg atatggtggt
      421 caatgccgcc gggatcctgc gcggcgctca gatggaggcg gtgcacgtgg ccatgccggt
      481 ggcgctgcac caggccgcga gcgaggccgg ggtcaggcgc gtggtgctga tttccgcgat
      541 cagcgcccgg cccgatgtgc cgaccgacta tgcccaatca aagctgcgcg gcgaagcagc
      601 gctgcgccag tgtggccttg gctggacgat cctgcgcccc tcgctggtct atggcgaagg
      661 ctcctatggc ggaacctcgc tgttgcgcgg gctggctgcg ctgccctggc gcgtgccgct
      721 gcccggacag ggccaattcg ccttctcgcc gatccatgtc cgcgatctcg cccgcgcggt
      781 gcgcgaagtc tgcgaggacg agcgccatgc cggccaggtg ctcgacccct gcggccccga
      841 aacgttgagc ctcagcgatc tgctgggccg ctatcggcgc tggctgggct ttggcgaggc
      901 ccgcttcgtg gccgtcccga tgcccgtgat gcgcctgctg ggccggattg gcgaccggct
      961 gggcgatggc ccggtttcaa ccaacagcct ggtgcagctg gtggccggca acgcgggcga
     1021 cggcgagggc tttgcccgga ccatcggttt tgccccgcgc agcctgggtg cggcgctgcg
     1081 cgaaagcccg gcgcaggttc aggaccgctg gcacgcccgg ctgttcttcc tcgcccccgc
     1141 gatccgctgg accctggcca tgctgtggct ggtatcggcg ctactggggc tgggctgggg
     1201 cgcggagcag gcccgggcgg tggtggcggg cctcgggctg ccgcaaggca tggctgatcc
     1261 gctgcgctgg ggcggctcgg ctctcgatct ggtgatcgcg gcgacggtgc tggccgatcg
     1321 gcgcggccgg atcgcaacag ggctgcaatt gctggcagtc gtcggctaca gtctggtgat
     1381 cggccttgcg atcccggcgc tgtggctcga tccgcttggc cccttgctca agaacctgcc
     1441 gatcctggta ttgatcctgg ttcacggcgc aatcgcggag cagcgctgat ggagcccttc
     1501 ctgctggtca aatggctgca catcctgtcc agcacggtgc tgttcggctt cggtgccggg
     1561 accgcctggt acatgtggaa cgcgcacctg ggcggcgatc cgcgcacgat tgccggcgtc
     1621 gcgcgaatgg tggtgcgggc cgactggatt ttcaccggga ccagcgggat cgtccagccg
     1681 gcaagcggcc tggtgctggc gcatatggcc ggctggccgc tcgacacacc ctggctggtg
     1741 gcaagctacg cgctctacct gctggccttc gtctgctggg ttccagtggt cggcctgcag
     1801 atccgcgccc gcgacctggc agcacacgcg gttgcgagcg gtaagccgct gggcgaggac
     1861 tatcgccgcg ccatgcgctg gtggtttgcg ctgggctggc ccgcctttct gggcctgctc
     1921 ggggtgttct ggctgatggt tgccaaaccc gcgctgtggt gagcggcaat cgcccccgcg
     1981 ggcaatatca tttcccgccc cgattc
//
LOCUS       DASUDV010000599         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_2054246,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000599 DASUDV010000000
VERSION     DASUDV010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054246"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..763)
                     /locus_tag="VFV30_13275"
     CDS             complement(2..763)
                     /locus_tag="VFV30_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4849110.1"
                     /translation="MITEPFLLAVIGGALIGLLLTLFGGGGSVLATPWLIYVVGMADV
                     HAAIGTSAAAVAVNAATGLAAQARAGRVKWPCAVVFGISGLAGALLGAHLAKQVDGAA
                     LLTGFAFAMIAIAVSMLLPRKSAGDPDVHLTGPMVLKLAPVGLAAGLAAGFFGIGGGF
                     LIAPGLMAATGMTLANAGASSLVSVTLFGTATSASYAASGLIAWPLFGALVLGGAGGT
                     LAAMPLAKKLEQRVRLARSLFALMVIAVALFILLR"
     gene            complement(768..1301)
                     /locus_tag="VFV30_13280"
     CDS             complement(768..1301)
                     /locus_tag="VFV30_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HEX4849111.1"
                     /translation="MSAVLHPLAPADVRARLAARTAVLVDIREADEFARVHVAGAVSQ
                     PLSRWERGHLTIDPAADVVFTCRSGMRTQGACDRLAARVEGDAYVLDGGLNAWIKAGL
                     PVTEDSKAPLEIMRQVQIAAGTLVLIGVLLGTFVAAPWYGLAGFVGAGLAFAGVTGFC
                     GMARLLMLAPWNRPRTA"
     gene            complement(1298..1630)
                     /locus_tag="VFV30_13285"
     CDS             complement(1298..1630)
                     /locus_tag="VFV30_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4849112.1"
                     /translation="MFDLTRFDPALFSESAGRAAELLRVLGNEKRLMVLCQLADGELS
                     VGEIQQRLGLSQSALSQHLAVLREQGVVATRRQAQTIFYRIEDHAALRVVQVLAELFC
                     PPEVRNPA"
     gene            1696..>2029
                     /locus_tag="VFV30_13290"
     CDS             1696..>2029
                     /locus_tag="VFV30_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4849113.1"
                     /translation="MSYAPPLVPQVQAFFDPATFTVSYVVHDPATKAAAIIDPVLDFT
                     PRNARTATGSADLLLDYVAAQGLKLEWLLETHAHADHLSAAHYLREKTGAPVVIGAAI
                     TQVQEAFAP"
BASE COUNT          361 a          722 c          682 g          264 t
ORIGIN      
        1 ctcagcgcag caggatgaac agcgccaccg cgatgaccat cagcgcgaac aggctccgcg
       61 cgaggcggac ccgctgctcc agctttttgg cgagcggcat ggcggccagc gtgccgcccg
      121 cgccgcccag caccagcgcg ccgaacagcg gccaggcgat caggcccgaa gcggcgtagc
      181 tggcgcttgt cgccgtcccg aacagggtga ccgagaccag cgaagaggcc ccggcgttgg
      241 ccagcgtcat gccggtggcg gccatcagcc ccggcgcgat caggaacccg ccgccgatcc
      301 cgaagaagcc ggccgccagc cccgcggcaa ggccgacggg cgcaagcttc agcaccatcg
      361 gcccggtcag gtggacatcg ggatcgcccg cgctctttct gggcagcagc atcgaaaccg
      421 cgatggcgat cattgcaaag gcgaacccgg tgagcagcgc cgcgccatcg acctgcttgg
      481 caaggtgcgc gcccagcagg gccccggcca ggcccgaaat cccgaagacg accgcgcagg
      541 gccacttgac ccgcccggcg cgggcctggg cggcaaggcc ggtcgcggcg ttgacggcca
      601 cggccgcagc cgaggtcccg atcgcggcgt ggacatcggc catgccgacc acatagatca
      661 gccagggggt ggccagcacc gatccgcccc cgccgaacag ggtcagcagc aggccgatca
      721 gggcaccgcc gatcacggcc agcaggaacg gttcggtgat catcggctca ggccgtccgc
      781 ggccggttcc acggcgccag catcagcagc cgcgccatgc cgcagaaccc ggtcaccccg
      841 gcaaaggcca gcccggcgcc gacaaagccg gccaggccgt accacggcgc ggcgacgaag
      901 gtgcccagca gcaccccgat caggaccagc gtcccggccg cgatctggac ctggcgcatg
      961 atctccagcg gggccttact atcttcggtc acgggcagcc cggccttgat ccaggcgttg
     1021 agcccgccgt cgaggacata ggcatcgcct tcaacccgcg cggccagcct gtcgcacgcg
     1081 ccctgggtgc gcatgccgct gcggcaggtg aagaccacgt cggcggcggg atcgatggtc
     1141 aggtggccgc gttcccaccg cgaaagcggc tgcgagaccg cgccggccac gtggacgcgg
     1201 gcgaattcgt cggcttcgcg gatatcgacc agaaccgccg tacgggcggc caggcgggcg
     1261 cggacatcgg cgggggcaag cgggtgcagg acggcgctca tgcggggttc ctcacttcgg
     1321 ggggacagaa cagttcggcc agcacctgga ccacccgcag cgcggcgtga tcctcgatcc
     1381 ggtagaagat ggtctgcgcc tgccggcgcg tggcgaccac gccctgttcg cgcagcacgg
     1441 cgaggtgctg cgacagggcc gactgcgaca gaccaaggcg ctgctggatc tcaccgacgg
     1501 agagttcccc gtcggcaagc tggcacagca ccatcaggcg cttttcgttg cccagcacgc
     1561 gcagcagctc tgcggcgcgg ccggcgcttt cggaaaagag cgcgggatcg aaacgggtca
     1621 ggtcgaacat gattcgcttt atatgcattg attctaaatt agcaagtgct aatataaggt
     1681 caaaggagac gaatcatgtc ctatgccccg ccattggtcc cgcaggtcca ggccttcttc
     1741 gatccggcca cgttcaccgt cagctatgtc gtccacgatc ccgccaccaa ggccgcggcg
     1801 atcatcgatc cggtgctgga cttcacgccc cgcaacgcgc gcaccgcaac cggctcggcc
     1861 gatctgttgc tcgactatgt cgcggcgcag gggctgaagc tggaatggct gctcgaaacc
     1921 catgcccatg cggatcacct ctcggccgcg cactacctgc gcgagaagac cggcgcgccg
     1981 gtggtgatcg gcgccgcgat cacccaggtg caggaagcct ttgccccgc
//
LOCUS       DASUDV010000600         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_838704,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000600 DASUDV010000000
VERSION     DASUDV010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838704"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..630
                     /locus_tag="VFV30_13295"
     CDS             181..630
                     /locus_tag="VFV30_13295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4849114.1"
                     /translation="MSDPSPPFVFDPARAAKIMTRHGHGGWLGLDYHAHGQGWVELAL
                     PWREELVGNAETGVLASGPIISLMDNATSMSVWTRSTNFVPHATLDLRVDYMRAAVPG
                     KTVIGRGECYKITRKIAFIRGIAYDGDPDHPVAHVTGTFMATHGTYF"
     gene            630..1025
                     /locus_tag="VFV30_13300"
     CDS             630..1025
                     /locus_tag="VFV30_13300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4849115.1"
                     /translation="MGWHLPPFAEALGIALDHDEDGAPVLCVDFSDKVRGRPGFLHGG
                     AMSGLMEMAGIAALRAELDGRGEAPQLKPVNIQVEFMRGGTEQRTFAKGTVTRAGRRV
                     ALVNVEAWQDDPKKPIALAQMKLLLSASE"
     gene            complement(1009..1770)
                     /locus_tag="VFV30_13305"
     CDS             complement(1009..1770)
                     /locus_tag="VFV30_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4849116.1"
                     /translation="MHSLALFHRIAGQPVIVLGEGEAAAAKRRLVERAGGVIVGEDDP
                     AARLAFIAMDAPEAPAARLKERGLLVNVTDRPELCDFTVPSLLERGPVLVAVATGGVS
                     AGLAKALRLRLEALLPQSLGLLAEALGAARQQLRGRWPDAAGRRRALDQALAAGGVLD
                     PLDAGSAGRVGSWLEQGSEPAPARLEFVLVSPDPDDLTLRQARALGEADRVIHDAAVP
                     AAILNRARADAVRLVLVPGQAAPEASGNVVIIRSR"
     gene            complement(1770..>2272)
                     /locus_tag="VFV30_13310"
     CDS             complement(1770..>2272)
                     /locus_tag="VFV30_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX4849117.1"
                     /translation="AEYGEMVGRLTRDWDTTLMFEPGRVICGNAGVLLTRVIRVKRSS
                     NGAPFVVVDAAMNDLARPAMYGAWHDFDAVRPNGSRMIANVVGPICETGDTFAMGRDI
                     DALAAGDLAVFRTAGAYGATMASSYNSRGFVCEVMVDGDKFAVVADRILPEAITAAER
                     VPDWLD"
BASE COUNT          390 a          800 c          753 g          329 t
ORIGIN      
        1 ccccggtaag ggtggaaaga tcgggcagca gcgatagatc gatgacaggc aatatcatgg
       61 cggtttcccc tatgttcgcg cagggcccct tgccccgtgc ctgccctgct tcccgcgcag
      121 ggcgcggcga atgaaattaa aagattgtaa tcagccgcgc ggctcagcat ggggacgggg
      181 atgagcgatc caagcccgcc attcgtgttc gatcccgccc gcgccgcgaa gataatgacc
      241 cggcacggcc acggcggctg gctggggctg gattaccacg cccacggcca aggctgggtc
      301 gaactggcgc tgccctggcg cgaggaactg gtcggcaacg ccgaaaccgg cgtgctggcg
      361 tcggggccga tcatcagcct gatggacaat gccacatcga tgtcggtctg gacccgctcg
      421 accaatttcg tgccgcacgc gacgcttgac ctgagggtcg actacatgcg cgcggccgtg
      481 ccgggcaaga cggtgatcgg gcgcggcgaa tgctacaaga tcacccgcaa gatcgccttc
      541 atccgcggga tcgcctatga cggggacccg gatcatccgg tggcccacgt gaccggtacc
      601 ttcatggcca cccacgggac ctatttctga tgggctggca ccttcccccc tttgccgagg
      661 cgctgggcat cgcgctcgat cacgacgagg acggcgcgcc ggtgctgtgc gtcgactttt
      721 ccgacaaggt gcgcggccgg cccggattcc tccacggcgg ggcgatgagc gggctgatgg
      781 aaatggccgg gatcgccgcg ctgcgcgccg aactggacgg gcgcggcgag gcgccgcagc
      841 tcaagccggt caacatccag gtcgaattca tgcgcggcgg caccgaacag cgcaccttcg
      901 ccaagggcac ggtcacccgc gccgggcggc gggtggcgct ggtcaatgtc gaggcctggc
      961 aggatgatcc gaaaaagccg atcgcgctgg cgcagatgaa gctgctgctc agcgcgagcg
     1021 aatgatcacc acattgccgc tggcttcggg cgcggcctga ccgggcacca gtaccagccg
     1081 cacggcatcg gcgcgggcgc ggttgaggat cgccgccggc acggcggcat cgtggatcac
     1141 cctatcggcc tcgcccagtg cgcgcgcctg acgcagggtc aggtcatccg gatcgggcga
     1201 aaccagaacg aattcgagcc gcgccggtgc cggttcgctg ccctgctcca gccaagagcc
     1261 aacccgcccg gcactgcccg catcaagcgg atcgagcacg ccgcccgcgg ccagcgcctg
     1321 atcgagcgcg cggcgccgcc cggccgcatc gggccaacgc ccgcgcagct gttgccgcgc
     1381 cgcgcccagc gcttcggcca gcaagcccag cgattgtggc agcagcgcct caagccgcag
     1441 ccgcagcgcc ttggccagac ccgccgaaac cccgccggtc gccaccgcaa ccagcaccgg
     1501 tccgcgttcg agcaggctgg gaacggtaaa atcgcacagt tctggacggt cggtgacgtt
     1561 caccagcaat ccgcgctcct tcagccgggc cgccggcgcc tcgggcgcgt ccatggcgat
     1621 gaaggccagc cgcgcggcgg gatcgtcttc gccgacaatc accccgccgg cgcgttcgac
     1681 caggcgccgc ttggctgcag ccgcctcgcc ctcgcccagc acgatcaccg gctggccggc
     1741 aatgcggtgg aagagcgcga ggctgtgcat cagtcgagcc agtccggcac gcgttcggca
     1801 gcggtgatcg cctcgggcag gatgcggtcg gcgaccaccg cgaacttgtc gccatcgacc
     1861 atcacctcac acacaaagcc gcggctgttg taggagctgg ccatcgttgc gccataggcc
     1921 ccggcggtgc ggaacaccgc cagatcgccg gctgccaatg catcgatatc gcggcccatg
     1981 gcaaaggtat cgccggtttc gcagatcggc ccgaccacgt tggcgatcat ccggctgcca
     2041 ttgggccgga ccgcgtcgaa atcgtgccag gcgccgtaca tggccgggcg cgcaagatcg
     2101 ttcatcgccg catcgaccac cacgaacggc gccccgttcg agctgcgctt gacccggatc
     2161 acccgggtca gcagcacccc ggcattgccg cagatcaccc ggcctggttc gaacatcaag
     2221 gtggtgtccc aatcgcgggt aagacggccg accatttcgc catattcggc cg
//
LOCUS       DASUDV010000601         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1811215,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000601 DASUDV010000000
VERSION     DASUDV010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811215"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="VFV30_13315"
     CDS             complement(<1..1491)
                     /locus_tag="VFV30_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4849118.1"
                     /translation="MIAFTFDGRAMQAQPGDTLAAALVRNGVQLVGRSFKYHRPRGIL
                     SAGVEEPNALVTVGEGGRTEPNTRASDVFVYEGMVATSQNAWPSLKFDLGAVIGLFSK
                     ALPAGFYYKTFFGPAKLWLGYEWFIRRAAGLGNAPTLPDPDRFAQRGAFCDVLVIGGG
                     PAGLAEALKAAEAGEKVILVEQDAVLAPSLRRDPQELTPAWAEVTAARIRELGGTVLT
                     RTSAAGYWEHDLVTLTQLLVEPGQVPGQGPAQRLWHVRAGRVVLASGALERPLAFASN
                     DLPGVMLSQAVRTYARRFGVVPGKRVVVATNNDDAYATAHALAELGAEIVAVLDSRPA
                     SAVPDLAGNLPLFTDARPIAARGGSGGVRALTARIGETEQTFATDLVAMSGGFTPTLH
                     LFMQAGSTLGWDDTAQAFIPGTSRQNITAVGAAAQVAPIHDAEPLGDPKKSFIDFQND
                     VTLGDVDLAWAEGYRSVEHVKRYTTLGMATDQGKTGNMAALTRLARH"
     gene            complement(1488..1751)
                     /locus_tag="VFV30_13320"
     CDS             complement(1488..1751)
                     /locus_tag="VFV30_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX4849119.1"
                     /translation="MMQIPCPHCGNRAQAEFVYERAAESVIVPGRDDDTAMERLYTRT
                     NPRGVDDELWRHTYGCRAWMVVTRSRVTHEITQVRAIGPEALA"
     gene            complement(1766..>2832)
                     /locus_tag="VFV30_13325"
     CDS             complement(1766..>2832)
                     /locus_tag="VFV30_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4849120.1"
                     /translation="KGYVGGGNTGRNTTLVRSNYRQKPLHDFFEFGMGLWRHLSDELN
                     YNVMFSPRGSMFLGHSDGDMIKLAELGDAMRTSGIDAELLSRDQVARQEPLLDMSRDA
                     RFPIEGGLIQRSGGTARHDAVAWGYARGADHLGVDIIQQCEVTGLVRQGDAVIGVETT
                     RGRIGAGKVGICVAGHSGHVAAMAGLRLPVESQTLQALVTEGVKPMIRSVIMSMSLHC
                     YISQSDKGGIVMGGDPDNWPSYAQRGHPMITEHAIAQAIALVPALSRLRMLRTWSGVT
                     DMSFDGAPIICPTPVPNLYLNGGWCYGGFKATPASGWTFAHTLGTGKAHPLNAPFDLD
                     RFKRGDTIDEPAAGPMPNHR"
BASE COUNT          435 a          942 c          988 g          467 t
ORIGIN      
        1 gtgccgggca agccgtgtca acgccgccat gttgccggtc ttgccctgat cggtggccat
       61 gcccagcgtg gtatagcgct tgacgtgttc gaccgagcga tagccttcgg cccaggccag
      121 atcgacatcg cccagagtta catcgttctg gaaatcgatg aagcttttct tgggatcgcc
      181 cagcggctcg gcatcgtgga tcggggcgac ctgtgccgcc gcgcccacgg cggtaatgtt
      241 ctgccgcgat gtgccgggga tgaaggcctg cgctgtatcg tcccagccga gcgtggagcc
      301 ggcctgcatg aacaggtgca gcgtgggggt gaaaccgccc gacatggcca caagatcagt
      361 ggcgaaggtc tgctccgtct cgccgatccg ggcggtcagc gcgcgcacgc cgccgctgcc
      421 gcccctggct gcgatcggcc gggcatcggt gaacagcggc aggttgccgg ccagatcggg
      481 caccgccgac gccgggcggc tgtcgagcac cgcgacgatc tctgccccca gttcggcaag
      541 ggcatgggcg gtggcatagg catcgtcgtt gttggtcgcc accaccacgc gcttgcccgg
      601 caccacgccg aagcggcggg cataggtgcg caccgcctgg ctcagcatga cgccgggaag
      661 atcgttgctg gcaaaggcca gggggcgctc cagcgcgccg ctcgccagca ccacgcggcc
      721 tgcgcgcacg tgccacaggc gctgggcagg gccttgcccg ggaacctggc cgggttcgac
      781 cagcagctgg gtcagcgtca ccagatcgtg ttcccagtaa ccggcggcgc tggtgcgggt
      841 cagcaccgtg ccgccaagct cgcggatccg cgcggcggtg acctcggccc aggcgggcgt
      901 gagttcctgc ggatcgcggc gcagcgaggg cgcgagtacg gcgtcctgtt caaccaggat
      961 caccttttcg cccgcctctg cggccttgag cgcttcggca aggcccgccg gaccgccgcc
     1021 tatcaccagc acgtcgcaga aggcgccgcg ctgggcgaag cgatcgggat cgggcagggt
     1081 gggcgcgttg ccaaggccgg cggcgcggcg gatgaaccat tcgtagccga gccacagctt
     1141 ggccgggccg aagaaggtct tgtaatagaa gcccgcgggc agcgccttgg aaaacaggcc
     1201 gatcaccgcg cccagatcga acttcaggct gggccaggcg ttctggctgg tggcaaccat
     1261 cccttcgtag acgaagacat cgctggcgcg ggtgttgggt tcggtccgcc cgccttcgcc
     1321 gacggtgacc agcgcgttgg gttcttccac cccggcggag aggatcccgc gcgggcggtg
     1381 gtacttgaag ctgcggccaa ccagttgcac gccattgcgc accagcgcgg cggccagcgt
     1441 gtcaccgggc tgggcctgca tcgcccggcc atcgaaggtg aaggcaatca tgccagcgcc
     1501 tccggcccga tcgcgcgcac ctgggtgatt tcgtgcgtca cgcggctgcg cgtcaccacc
     1561 atccaggcgc ggcagccata ggtgtggcgc cacagttcat catccacccc gcgcgggttg
     1621 gtgcgggtgt agaggcgctc catcgccgta tcgtcatcgc gccccgggac gatgaccgat
     1681 tcggcggcgc gttcgtagac gaattcggcc tgggcgcggt tgccgcagtg cgggcagggg
     1741 atctgcatca tgggtctggg ttcccctagc ggtggttggg catcggcccg gcggccggtt
     1801 cgtcgatcgt gtcgccgcgc ttgaagcgat cgagatcgaa cggggcgttg agcgggtgcg
     1861 ccttgccggt gccaagcgtg tgggcaaagg tccagcccga agccggggtc gccttgaagc
     1921 cgccatagca ccagccgccg ttgagataga ggttcggcac cggagtgggg cagatgatcg
     1981 gcgcgccatc gaagctcatg tcggttacgc ctgaccaggt gcgcagcatc cgaagccgcg
     2041 acagcgcggg gaccagcgcg atcgcctggg cgatggcgtg ttcggtgatc atcgggtggc
     2101 cgcgctgggc gtagcttggc cagttgtcgg gatcgccgcc catcacgatc ccgcccttgt
     2161 cggactggct gatgtagcag tgcagcgaca tcgacatgat cacgctgcgg atcatcggct
     2221 tgaccccttc ggtcaccagc gcctgcagcg tctggctttc gaccgggagc ctgagcccgg
     2281 ccatcgcggc aacatggccg ctgtgcccgg caacgcagat gccgaccttg cccgcgccga
     2341 tccggccgcg ggtggtctcc accccgatca cggcatcgcc ctggcggacc agcccggtca
     2401 cttcgcactg ctggatgata tcaacgccca gatgatcggc gccgcgggca tagccccagg
     2461 ccaccgcatc gtggcgggcg gtgccgccgc tgcgctggat cagcccgcct tcgatcggga
     2521 agcgggcatc gcgcgacata tcgagcagcg gttcctgccg ggccacctga tcgcggctca
     2581 agagctcggc atcgatcccg ctggtgcgca tcgcatcgcc gagttcggca agcttgatca
     2641 tgtcgccatc gctgtggccc aggaacatcg agccgcgggg tgagaacatc acgttgtagt
     2701 tcagctcgtc cgacaggtgc cgccacagcc ccatgccgaa ttcgaagaaa tcgtgcagcg
     2761 gcttctggcg atagttcgac cgcaccaggg tggtgttgcg cccggtgttg ccgccgccga
     2821 catagccctt tt
//
LOCUS       DASUDV010000602         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1479520,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000602 DASUDV010000000
VERSION     DASUDV010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479520"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFV30_13330"
     CDS             <1..1115
                     /locus_tag="VFV30_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4849121.1"
                     /translation="QTGALVTALGFGLSAFVLLAVIQTSLEANIASRVPARAPDYFML
                     DLPRERAAEMEQIVRQAAPQAKVRQIPALRGAILAYGPEAAMTRVADLKEIPEDAWPL
                     RGERGLTYSATLPEGNSLTAGQWWPKDYTGEPLVSVDEELAKAINLKLGDKITIGVLG
                     VERSARIASFRRIDWDSMGFNYVLVFSPNTLADAPHNLAATIDLPAGADRTRLLPTLL
                     KAFPASSVIEVGGVLKQARELLGQVSTAILAAASVAVLAGIAVLLGAIAAARASRLYD
                     NVVLRVLGASRRHLLLLQLAEYGLLALLLAGVALVLGSAIGWLVIVQLFKFDWLPNWP
                     QVLAVLAAGLGMTVAFALAGSLPLLRAKPAQALREL"
     gene            1175..1753
                     /locus_tag="VFV30_13335"
     CDS             1175..1753
                     /locus_tag="VFV30_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX4849122.1"
                     /translation="MFIETETTPNPATLKFLPGQEVMPSGTRDFRDEEEAAASPLAEA
                     LFSLGDVTGVFFGFNFVSVTAGEGADWSALKPQVVAILLDHFVSQAPLFHPTGGNGIA
                     VPAEDDADYGDDPADADVVAQIKELLETRIRPAVANDGGDIAYRGFREGVVYLSMQGA
                     CSGCPSSTATLKHGIESLLKHYVPEVSEVRAA"
     gene            1879..>2038
                     /locus_tag="VFV30_13340"
     CDS             1879..>2038
                     /locus_tag="VFV30_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4849123.1"
                     /translation="MSQPLSDAALDQLFRTARTYNGYLDKPVTTEQLHAIWDLMKFGP
                     TSANMLPAR"
BASE COUNT          323 a          690 c          663 g          362 t
ORIGIN      
        1 cgcagaccgg ggcgctggta acggcgctgg gcttcggact ttcggccttc gtgctgctcg
       61 cggtcatcca gaccagcctt gaggccaata tcgccagccg cgtgcccgcc cgcgcgccgg
      121 attacttcat gctcgacctg ccgcgcgagc gcgccgcgga gatggagcag atcgtccgcc
      181 aggccgcgcc ccaggccaag gtccgccaga tcccggcgct gcgcggcgca atcctggcct
      241 atggccccga agcggcgatg acccgtgttg ccgatctcaa ggaaatccca gaggacgcct
      301 ggccgctgcg cggcgaacgc gggctgacct attcggccac cctgcccgaa ggcaattcgc
      361 tcaccgccgg gcagtggtgg ccgaaggatt ataccggcga gccattggtc tcggtcgacg
      421 aggaactggc caaggcgatc aacctcaagc tgggtgacaa gatcaccatc ggcgtgttgg
      481 gggtggagcg cagcgcacgg atcgcctcgt tccggcggat cgactgggat tcgatgggct
      541 tcaactatgt cctggtcttc tcgcccaaca ccctggccga tgccccgcac aacctggccg
      601 caacgatcga cctgcccgcc ggggccgacc gcacccggtt gctgccgacc ttgctgaagg
      661 cctttccggc cagctcggtg atcgaggttg gcggggtgct gaagcaggcg cgcgaactgc
      721 tgggccaggt ctcgaccgcg atcctcgccg cggcctcggt cgcggtgctg gccgggatcg
      781 ccgtgctgct gggtgccatc gccgccgcgc gggcgagcag gctctacgac aatgtcgtgc
      841 tgcgggtcct tggggcgagc cgccggcacc tcctgctgct gcaactcgcc gaatacggcc
      901 tgctggcgct gctgctggcg ggcgtggcgc tggtcttggg ttcggcaatc ggctggctgg
      961 tgatcgtgca gttgttcaag ttcgactggc tgcccaactg gccgcaggtc ttggccgtac
     1021 tggctgcggg gctgggcatg accgttgcct tcgcgctcgc cggttcgctg ccgctgctcc
     1081 gcgccaagcc ggcgcaggcc ctgagggaac tgtgaggcgc gcgaactgtg aatttggcgg
     1141 ttgaacccgc ccggtaaagg cctacatccg ccccatgttt atcgagaccg aaaccacccc
     1201 gaatccggcc acgctgaagt tcctgcccgg gcaggaagtg atgccatccg gcacccgtga
     1261 ctttcgcgat gaggaggagg ctgccgcctc tccgctggcc gaggcgctgt tcagcctggg
     1321 cgacgtgacc ggcgtgttct tcggcttcaa cttcgtctcg gtcaccgccg gtgaaggggc
     1381 cgactggtcg gcattgaagc cgcaggtcgt cgcgatcctg ctcgatcact tcgtttcgca
     1441 ggccccgctg ttccacccca ccggcggcaa cggtattgcc gtgccggccg aggacgacgc
     1501 cgattatggc gacgatccgg ccgatgccga tgtcgtcgcg cagatcaagg aactgctcga
     1561 aacgcggatt cgcccggccg tggcgaacga cgggggcgat atcgcctacc gcggcttccg
     1621 cgagggcgtg gtctatctct cgatgcaggg ggcctgctcg ggctgtccgt catcgaccgc
     1681 cacgctcaag cacggcatcg aaagcctgct caagcactac gtgcccgaag tgagcgaagt
     1741 ccgcgccgcc tgaacagggc tctcatcacc atttatcgtc attgcgagcg tagcgaagca
     1801 atccagagcg tcattgcaaa accctggatt gcttcgctac gctcgcaatg acgaacttta
     1861 ctgcagggga tcaatgccat gtctcagccg ctcagcgatg ctgcgctcga ccagctgttc
     1921 cgcaccgcgc ggacctataa cggctatctc gacaagccgg tcaccaccga gcagctccat
     1981 gcgatctggg acctgatgaa gttcggcccg accagcgcca acatgctgcc cgcgcgcc
//
LOCUS       DASUDV010000603         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1943835,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000603 DASUDV010000000
VERSION     DASUDV010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943835"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1579)
                     /locus_tag="VFV30_13345"
                     /pseudo
     CDS             complement(1..1579)
                     /locus_tag="VFV30_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415025.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0071513 - phosphopantothenoylcysteine
                     decarboxylase complex [Evidence IEA];
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
     gene            1677..2075
                     /locus_tag="VFV30_13350"
     CDS             1677..2075
                     /locus_tag="VFV30_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849124.1"
                     /translation="MRLILTALLFLFGLFNLLLGIGFLLDPASMQGNFPVTGNGPAGM
                     AVLRADLTAFFLVAGGCQLWGGWRRNGDLLLVPGLLMGIAFTGRLVSAAMDGTAPGFA
                     VPMVVEAVQVILAYAAWRVLPHHRISEIAN"
     gene            complement(2072..>2414)
                     /locus_tag="VFV30_13355"
     CDS             complement(2072..>2414)
                     /locus_tag="VFV30_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX4849125.1"
                     /translation="DWLGVVLAALAASAVLALAFRQARTGWLYAAVPLNLVSALMLGH
                     ALARIGPEKLAAKPQLFFMQSGGLALAFVIPALWLTQLRDGAPRRQTVRDSLAFLAAY
                     LAMGAVFWLRG"
BASE COUNT          381 a          798 c          815 g          420 t
ORIGIN      
        1 acctcgtcct tgggcagttc gggcaagtgg tcaacgccct tgctcgaaac gatgtggacg
       61 aggttcatgt cgccccccat cacgctgccc gaaacgtcat tggcgacgat ccagtcggca
      121 cccttgcgct tgcgcttgtc cttggcgtgt tccagcacct tctcggtctc agccgcaaag
      181 ccgacgacca gcttgggtcg cttgtcgctg accgagacca tcgcgaggat atccgggttc
      241 tcggtcagcc gcagcgccgg cggggccgag ccgcgcttct tcatcttttc gggcgcatag
      301 tcctcggtcc gccagtcggc gacagccgcg accatgaccg cggcatcggc tggcagcgaa
      361 cgtcgcaccg cctcggccat ctcgcgcgcg gtttcgacat cgacgcggtc cacgcccggc
      421 ggggtcggca gcgccacagg accagagacg agcgttaccc gcgcaccggc gcgggccgcg
      481 gcggcggcaa tggcatagcc ctgcttaccc gaggatcgat tggcgatgta gcggaccgga
      541 tcgatcggct catgcgtcgg cccggcggtg acgagcacgt gcttgccgta aagcgggcga
      601 tgggccggat cttcctcgaa atcaggctgg ccgacgagcg gatcggcttc tggcgcaact
      661 tcggccgggg cggctgccgg ggcatcgccc ttgagcggtt cgggcggggc ctggttcatc
      721 accaggtggt tgatggcctc ggcgtcggtc ggcggggcgg cgcggccttg cccttcttga
      781 ccaggatcgg accggcatcg cccgggacag gatcgggcag cggcacgaag ggtgccgggc
      841 cgtcaaagtt ggcatcgggg accacatcct cgacttcggc atcctcgggc agcagctcgt
      901 cttcgaacac gatccgcgag gtcgtgctgc gctggatcag gctgctggac aggccgccaa
      961 ggtctgctgc accggcaagc gggtcttccg gctcccaggt cagctcgggc acggcgctgg
     1021 gcagcagctc catcgagagc gtgtcgcaga ttgcgcgcat cacttcgatc ggttcgggca
     1081 ggcgtccggg gccgaactcg ccgcaagcca tcgggccttc gtcaggctcc atcacggcaa
     1141 tgccgcgttc gcgcagcgca gcgatattgg cttgggtcgc ggcgtgttcc cacatccgca
     1201 cgttcatcgc cggaacggcc attaccggct tgtcggtcgc cagcagcaga gtggttgcca
     1261 gatcgtcggc gatcccgtgg gtcatcttgg cgaggagatc ggccgttgcc gggcagacca
     1321 cgatcaggtc ggcctggcgc gagagctgga tgtgccccat ctcgacctcg ttcttgaggt
     1381 cccagagcga ggtgtggacc gggttctcgc tcagtgccgc cagcgtcatc ggcgtgacga
     1441 actgcgcgcc gccattggtg aggacgcaag ttacctcacc gccatgcttg cggatcagcc
     1501 gcacgagctc gcacgacttg taggcggcaa tcccgccgcc aataaccaga agaatccttg
     1561 ggccagtcat ggccaccagc ttgtgcaatg ccccatcccc ttgcgcaaca ggctttgctt
     1621 tcggatttcc gtggggcaca aaggttaaga tcgtcaaaac ggggaaagtt accgccatgc
     1681 gcctgatcct gaccgccctc ctgttcctgt ttggcctgtt caaccttttg ctggggatcg
     1741 gcttcctgct cgatccggct tcgatgcagg gtaattttcc ggtgacaggc aatggcccgg
     1801 ccgggatggc ggtgctgcgc gccgatctga ccgcgttctt cctggtcgcc ggggggtgcc
     1861 agctgtgggg cggctggcgg cgaaatggcg atctgctgct ggtccccggt ctgctgatgg
     1921 gcatcgcctt caccggccgg ctggtgagcg cggcaatgga tggcaccgca cccgggtttg
     1981 ccgtgccgat ggtggttgaa gccgtgcagg taatcctggc ctatgccgcg tggcgcgtgc
     2041 tgccgcacca ccgaatcagc gaaatcgcga actagccgcg cagccagaac accgcaccca
     2101 tggcgagata ggcggccagg aaggccaggc tgtcgcggac ggtctggcgg cgcggcgcgc
     2161 catcgcgcag ctgggtcagc cacaaggccg gaatcacgaa ggccagcgcc agcccgcccg
     2221 attgcatgaa gaacagctgc ggcttggcgg cgagcttttc cggcccgatc cgcgcgagcg
     2281 cgtggccgag catcagcgcc gagaccaggt tcaacggcac ggcggcataa agccatccgg
     2341 tccgcgcctg ccggaaagcc agggccagca cggcgctagc cgccagcgcc gccagaacca
     2401 cgcccagcca gtcc
//
LOCUS       DASUDV010000604         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_330999,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000604 DASUDV010000000
VERSION     DASUDV010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330999"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFV30_13360"
     CDS             complement(<1..907)
                     /locus_tag="VFV30_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849126.1"
                     /translation="MYDTLLLANLLLWLALAAHFFTRPNASVYHPAVYYLFFHGLIFV
                     FRPFVAYYRDYNLIYRAYEFIPTAEDKETVLIAAMLGLVCFYFAAVQSGTAPVRFPQD
                     RVTEAERSEMLRPYLIVAALLAPIGLVSVLANWVTRAGDSSTMAMDAATGRLINTTGN
                     GYFDDAQLMLAPLAVMCVWLFRFRWWSFLPLIAFVILRSGTGGRWPMVMACATVALLY
                     LYQSRRLWPDWRSLAIGAGVLTLFQTVGADRGTSIRRLFIEDRSLGERGADAVRELRF
                     LEGMDFANLEYFEYLVYVVPQRSGTW"
     gene            complement(900..2033)
                     /locus_tag="VFV30_13365"
     CDS             complement(900..2033)
                     /locus_tag="VFV30_13365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX4849127.1"
                     /translation="MSTGRFASGLRGLVSLKTTSDELLHLDLMRFIASAAIVLCHSGE
                     FFVPRASRPESFERLAGLSLFVDVFFIISGFVIAHIYADRMGTWRQFARFMQRRIGRL
                     FPLHLATLFAVGLLFYLIGVFNVPTNTDMRLVPECLVLGTLLLHSVIDCGGPVPNGVN
                     WSISAEMVMYALFPLLLLAARSLGLLRYGLWLVFLIGVSWTYAEGALWASKTEFLRAL
                     PAFFFGLVLRLDWERLDRFAVPGMVPVGFALALIAGSLLVWPGWLLLALAYAAAASAI
                     LADTRPAAAPLTRRLAPLGQLTYSMYMLHPIIIMVLVNAVGDKLLKLDPVPLGLVTLA
                     SYGLIMVVSLLSYKWFEQPARAFIDGLPLVPPERAKGGNEGHV"
     gene            complement(2030..>2316)
                     /locus_tag="VFV30_13370"
     CDS             complement(2030..>2316)
                     /locus_tag="VFV30_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849128.1"
                     /translation="CVRIVYCELCVAPEAVLRLALEGLGLGLEFDPAMLDFADKDHNF
                     GLEDPVVRGTRTITANSGAWRGLEPAQQARIRETFGDWAETSQPPARRTA"
BASE COUNT          470 a          768 c          779 g          299 t
ORIGIN      
        1 cccaggtgcc gctgcgctgg ggcaccacat agaccaggta ttcgaagtat tcgaggttgg
       61 cgaagtccat cccttccaga aagcgcagct cgcgcacggc atcggcgccg cgctcgccca
      121 ggctgcggtc ctcgatgaac agccggcgga tcgacgtgcc gcgatcggcg cccacggtct
      181 ggaacagggt cagcacgccc gccccgatcg ccaggctgcg ccagtccggc cacagccgcc
      241 gcgattggta cagatagagc agcgccaccg tggcgcaggc catcaccatc ggccagcgcc
      301 cacccgtccc gctgcgcagg atcacgaagg caatcagcgg caggaacgac caccagcgaa
      361 accggaacag ccagacgcac atcacggcca acggggcgag catcagctgg gcatcgtcga
      421 aatagccgtt gccggtggtg ttgatcaggc gcccggtggc ggcgtccatc gccatcgtgc
      481 tgctgtcccc ggcgcgggta acccagttgg caaggaccga aaccagcccg atcggtgcca
      541 gcagggcggc cacgatcagg taggggcgca gcatctcgct gcgttcggcc tcggtcacgc
      601 ggtcctgcgg gaaccgcacc ggcgctgtcc cgctttgcac ggcggcgaaa tagaagcaga
      661 ccaggccgag catcgcggcg atcagcacgg tctccttgtc ctccgccgtg gggatgaatt
      721 cataggcgcg gtagatcagg ttgtagtcgc ggtaataggc cacgaagggc cggaacacga
      781 agatcagccc gtggaagaac aggtaataga ccgccgggtg gtaaaccgaa gcgttcggcc
      841 gcgtgaagaa gtgggccgcc agggccagcc acagcagcag gttggccaga agcagcgtgt
      901 catacatgcc cttcgttccc ccccttcgcc cgctccggcg gaaccagcgg cagcccgtcg
      961 atgaacgccc gggccggttg ctcgaaccat ttgtaggaca ggagcgatac caccatgatc
     1021 agcccatagc tggccagcgt gaccaggccc agcgggacgg gatcgagctt cagcagcttg
     1081 tcacccaccg cgttgaccag aaccatgatg atgatcgggt gcagcatgta catcgaatag
     1141 gtcaactggc ccagcggagc cagccggcgg gtcagcggcg cggcggccgg gcgcgtatcg
     1201 gccaggatcg cgctggccgc cgccgcatag gccagcgcca gcaggagcca gcccggccag
     1261 accagcagcg aaccggcaat cagggccagg gcaaagccga ccggaaccat gccagggacg
     1321 gcaaagcggt ccagccgctc ccaatcgagc cgcagcacca ggccaaagaa gaacgccggc
     1381 agcgcgcgca ggaattcggt cttgctggcc cacagcgccc cttcggcata ggtccacgaa
     1441 acgccgatca ggaagaccag ccacaggcca tagcgcagca gccccaggct gcgggccgcc
     1501 agcagcagca gcgggaagag ggcgtacatc accatttccg cgctgattga ccagttgacg
     1561 ccgttgggca ccggcccgcc gcaatcgatc accgaatgga gcagcaaggt gcccagcacc
     1621 aggcattccg gcaccagccg catgtcggtg ttggtgggca cgttgaacac gccgatcagg
     1681 tagaacagca gtcccacggc gaacagcgtg gccaggtgca gcggaaagag ccggccgatc
     1741 cggcgctgca tgaaccgggc gaactggcgc caggttccca tccggtcggc atagatgtgg
     1801 gcgatgacga agccggagat gatgaagaac acatcgacga acagcgacag gccggccagc
     1861 cgctcaaagc tttcgggacg gctggcgcgc gggacgaaga actcgcccga atggcacagc
     1921 acgatggcgg ccgaggcgat gaaccgcatc aggtcgagat gcagcagctc gtcgctggtc
     1981 gtcttgagcg agaccagccc ccgcagtccc gaggcgaagc ggccggtgct catgcggtcc
     2041 ggcgcgccgg gggctggctg gtctcggccc agtcgccgaa ggtttcgcgg atccgggcct
     2101 gctgggcggg ctcaagcccg cgccaggcgc cgctgttggc ggtgatcgtg cgggtgccgc
     2161 ggaccaccgg gtcttcaagg ccgaaattgt ggtccttgtc ggcgaagtcg agcatcgcgg
     2221 gatcgaattc caggccaagg cccagccctt ccagcgccag acgcagcacg gcttcgggcg
     2281 cgacgcacag ctcgcaatag acgatccgga cgcagc
//
LOCUS       DASUDV010000605         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_883272,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000605 DASUDV010000000
VERSION     DASUDV010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883272"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFV30_13375"
     CDS             complement(<1..422)
                     /locus_tag="VFV30_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4849129.1"
                     /translation="MRRAGLALALLAASPLLAEDDPKTATEYVVKSGETLGGIANRVE
                     VPRVLIIEANGLKAPYTVRAGQKLVIPRRRSHTVQDGETGLSIALDYGVPWTAIAAAS
                     GIDPKKPIKPGQKLVIPTIAKGAGSSPPPTPAPAPGPSP"
     gene            complement(422..1183)
                     /gene="surE"
                     /locus_tag="VFV30_13380"
     CDS             complement(422..1183)
                     /gene="surE"
                     /locus_tag="VFV30_13380"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX4849130.1"
                     /translation="MRILLTNDDGIFAPGLTVLEAIARQFSDDIWIVAPAEEQSGAGH
                     SLTLSRPVRLRQHEERRFSVSGTPTDAVMMGLREVLPEAPDLILSGVNRGANLGDDVT
                     YSGTVSAAIEGALAGIRSIALSQVYASEGMGDAVPFGAAEAWGAKVIAPLLDLPFAPR
                     TLVNVNFPALPADKVQGIRVVRQGFHDYSRGTIVEGVDPRGYRYFWFGLDGIEHTPGH
                     ATDLEAIQDGFVAVTPLQLDLTHEASLAELAGRFA"
     gene            complement(1183..>2008)
                     /gene="serS"
                     /locus_tag="VFV30_13385"
     CDS             complement(1183..>2008)
                     /gene="serS"
                     /locus_tag="VFV30_13385"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX4849131.1"
                     /translation="FETGALISGARFTFLRGQMARLQRALAQFMLDWQTAENGYTECS
                     VPLLVNDASMYGTGQLPKFKEDLFGTTDGRWLIPTAEVSLTNSVREQILDGAALPLRL
                     TALTPCFRSEAGAAGKDTRGYIRQHQFEKVELVTICAPDQSEAEHERMTAAAESILQA
                     LELPYRKVLLCAGDMGFTANKTFDLEVWLPGQNAYREISSISNCGDFQARRMNARFRA
                     EEKAKPEFVHTLNGSGLAVGRTLVAVLENYQQEDGSVEIPTALLPYMGGIDRLVPA"
BASE COUNT          344 a          666 c          678 g          320 t
ORIGIN      
        1 ggggaggggc cgggcgccgg agccggtgtc ggcggcgggc tgctgcccgc acccttggcg
       61 attgtcggga tgaccagctt ctgccccggc ttgatcggct tcttggggtc gatcccgctg
      121 gccgcggcta tcgcggtcca tggcacgcca tagtccagcg cgatcgacag gccggtttcg
      181 ccgtcctgca cggtgtgact gcgccggcgc gggatgacca gcttctgccc ggcgcggacg
      241 gtataggggg ccttgaggcc attggcctcg atgatcaaca cccgcggaac ttcaacccgg
      301 ttggcgatcc cgcccagcgt ctcgccggat ttgacgacat attcggtcgc ggtcttggga
      361 tcgtcctcag ccagcagcgg acttgcggcc agcagggcca gtgcaagacc ggcccgccgc
      421 atcaggcgaa gcgccccgcc agctcggcca gcgaagcctc gtgcgtcagg tcaagctgca
      481 agggggttac cgccacaaag ccatcctgga ttgcctcaag atcggtcgca tgaccggggg
      541 tatgttctat cccatcaagg ccgaaccaga aatagcggta gccgcgcggg tccacacctt
      601 cgacgatggt gccgcgcgaa tagtcgtgga agccctggcg gaccacgcga atgccctgaa
      661 ccttgtcagc aggcagcgcg gggaagttga cattgaccag cgtgcgcggc gcaaacggca
      721 ggtcgagcag cggggcaatg accttggccc cccaggcctc ggcggcgccg aacggcacgg
      781 catcgcccat gccttcgctg gcatagacct ggctcagcgc gatcgagcga atcccggcca
      841 gcgcgccctc gatcgcggcc gagacggtgc ccgaataggt cacgtcatcg ccgaggttgg
      901 cgccgcgatt gacgcccgac aggatcaggt ccggcgcctc gggcaagacc tcgcgcaggc
      961 ccatcatcac cgcatcggtc ggcgtcccgg ataccgaaaa gcgccgctct tcatgctggc
     1021 gcagccgcac cgggcgcgac agggtgagcg agtggcccgc gcccgactgt tcctcggccg
     1081 gggcaacgat ccagatatcg tccgagaact gccgcgcgat cgcctccagc acggtcaggc
     1141 cgggggcgaa tatgccatcg tcgttggtga ggaggatgcg catcaggccg gcaccagccg
     1201 gtcgatcccg cccatatagg gcagcaaggc cgtcggaatt tcgaccgaac cgtcttcctg
     1261 ctggtaattc tccagcacgg ccaccagcgt ccggccaacc gccaggcccg agccattgag
     1321 cgtgtggacg aattccggct tggccttctc ctcggcgcgg aaccgcgcgt tcatccggcg
     1381 ggcctggaaa tcgccgcagt tggagatcga gctgatctcg cggtaggcgt tctggccggg
     1441 cagccaaacc tcaagatcaa aggtcttgtt cgcggtaaag cccatgtcgc cggcgcagag
     1501 caggaccttg cgatagggca gctccagcgc ctggaggatc gattccgccg cggcggtcat
     1561 ccgctcatgc tcggcctcgg actggtcggg cgcgcagatt gtcaccagct cgaccttctc
     1621 gaactggtgc tggcggatat agccgcgggt gtccttgccg gccgcgcccg cttcggagcg
     1681 gaagcagggg gtcagcgcag tcaggcggag cggcaaggcg gcgccatcga ggatctgctc
     1741 gcgcaccgag ttggtcagcg aaacctcggc agtcgggatc agccagcggc catccgtggt
     1801 gccgaacagg tcctccttga acttcggcaa ctgcccggtg ccgtacatgg aggcgtcgtt
     1861 caccagcagc ggcacggagc attcggtgta gccgttctcg gccgtctgcc agtcgagcat
     1921 gaattgggca agcgcacgct ggagccgcgc catctggccg cgcaggaaag tgaaacgcgc
     1981 acccgaaatc agcgcgccgg tctcgaag
//
LOCUS       DASUDV010000606         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1524229,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000606 DASUDV010000000
VERSION     DASUDV010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524229"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFV30_13390"
     CDS             complement(<1..398)
                     /locus_tag="VFV30_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849132.1"
                     /translation="MPDKRLGLVAAALLALTSPLGARPEAVEAALAAASNAAAQGQGI
                     KAEAELRKALSAGASRSDVAARMGEAYLLQGDLRNARDWLEPAQFSKADEALGWRMTG
                     RLLRLEGRLPEAGKAFDKALTLTPNDPLLWV"
     gene            complement(391..1755)
                     /gene="prsR"
                     /locus_tag="VFV30_13395"
     CDS             complement(391..1755)
                     /gene="prsR"
                     /locus_tag="VFV30_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX4849133.1"
                     /translation="MSDTKPKLLIVEDDAGLQAQLKWAYPDFSVIIAGDRASALAALR
                     AEEPAVVTLDLGLPPDPDGTSEGFAVLDEIMALKPDTKVIVASGHGARESALQAIARG
                     AYDFYQKPVDIETLGLIVQRAFRLHQIEAENRQLASKSDTGNQVLGRLITGAPEMVKV
                     ARTIERVANTNVSVMLLGASGTGKELLARGLHDASDRAGRAFVAINCAAIPENLLESE
                     LFGHEKGAFTGAVKTTEGKIEQAQGGTLFLDEVGDIPLPLQVKLLRFLQERVIERIGS
                     RKSIPVDTRIVCATHQDLEAMIAAGSFRDDLFYRLAEIVVKIPSLAERPGDAGLLAKA
                     FLARFAREMNPQVKGFAADALAAIDAWHWPGNVRELENRVKRAVIMSEGKLVGAADLD
                     LADKELEDDDVLNLKSARETTDRRVIRHALARCEGNISNTAKLLGISRPTLYDLLKQY
                     DLHA"
     gene            complement(1755..>2216)
                     /locus_tag="VFV30_13400"
     CDS             complement(1755..>2216)
                     /locus_tag="VFV30_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4849134.1"
                     /translation="PVAADAVAKRVAAQFKASHPVELVQAEPCLVAGREEVIEQVLVH
                     LIQNAIDASPPDSPVFIQVGIEQFYGVIEVVDSGSGMSAEFVRTGLFKPFVSTKAGGF
                     GIGAYEARELVKAMGGQLAVESREGLGSRFSIRLPLAEAAELLNNFAAKVA"
BASE COUNT          354 a          786 c          675 g          401 t
ORIGIN      
        1 acccacagca aggggtcgtt cggcgtcagc gtcagtgcct tgtcaaaggc cttgcccgcc
       61 tcgggcaacc tgccctccag ccgcagcaga cgccccgtca tgcgccaacc gagcgcctcg
      121 tcagccttgg agaactgagc cggttccagc cagtcgcggg cattgcgcag gtcgccttgc
      181 agcagatagg cttcgcccat ccgcgcggcc acgtcgctgc ggctggcgcc ggccgacaac
      241 gccttgcgca gttccgcctc ggccttgatc ccctgtccct gcgcggccgc attgcttgcc
      301 gccgccagcg ctgcctcgac cgcttcgggc cgcgcgccca gcgggctggt cagcgccagc
      361 agcgccgccg caaccaaccc gaggcgcttg tcaggcatgg agatcgtatt gcttgagcag
      421 gtcatacagc gtcgggcgac tgatcccgag cagcttggcc gtgttcgaga tattgccctc
      481 acatcgcgcc agcgcatggc ggatgacgcg gcggtcggtc gtctctcgcg ccgatttgag
      541 gttgagcacg tcatcgtcct caagctcctt gtcggccagg tccagatcgg ccgcgccgac
      601 cagcttgcct tcgctcatga tgacggcgcg cttgacccgg ttttccagct cgcggacgtt
      661 gcccggccag tgccaggcat cgatcgcggc cagggcatcg gcggcaaagc ccttgacctg
      721 cgggttcatc tcacgcgcga accgcgccag gaaggccttg gccagcaggc cggcatcgcc
      781 gggccgctct gccaggctgg ggattttcac gacgatttcg gccaggcgat agaacaggtc
      841 atcacggaaa ctgccggcag cgatcatcgc ctccagatcc tggtgggtgg cgcagacaat
      901 ccgcgtatcg accgggatcg acttgcgcga tccgatccgc tcgatcaccc gctcctgcag
      961 aaagcgcagc agcttgacct gcagcggcag cggaatgtcg cccacttcgt ctaggaacag
     1021 cgtgccgccc tgggcctgtt cgatcttgcc ctcggtggtc ttgaccgccc cggtgaaggc
     1081 gcccttctca tggccgaaca attcgctctc gagcaggttc tcgggaatcg ctgcgcagtt
     1141 gatcgccaca aaggccctcc ctgcgcggtc gcttgcatcg tgcaggccgc gggcgagcaa
     1201 ttccttgccg gtcccgcttg cacccagcag catgaccgaa acgttggtat tagccacccg
     1261 ttcgatcgtg cgggccacct tgaccatttc cggcgccccg gtgatcagcc ggcccagcac
     1321 ctgattgccg gtgtcgctct tgcttgccag ttggcggttc tccgcctcga tctggtggag
     1381 ccggaaagcg cgctggacga tcaggcccag cgtctcgata tcgaccggct tctggtagaa
     1441 atcataggct ccgcgcgcga tcgcctggag cgccgattcc cgcgcaccgt ggcccgaggc
     1501 aacgatcacc ttggtgtcgg gcttcagcgc catgatctcg tccagcacgg caaagccttc
     1561 gctggttccg tccggatcgg gcggcaggcc caggtcgagc gtgaccacgg ccggctcttc
     1621 cgcccgcagc gccgccagcg ccgaggcgcg atccccggcg atgatcaccg agaaatcggg
     1681 ataggcccat ttcagctggg cctgcaggcc ggcatcgtcc tcgacgatca gcagcttggg
     1741 tttggtgtca ctcatcaggc taccttcgca gcaaaattgt tcagcagttc ggccgcttcg
     1801 gccagcggca ggcggatcga gaagcgcgaa cccagccctt cgcggctttc caccgccagc
     1861 tgcccgccca tcgccttgac cagttcacgc gcctcatagg caccgattcc gaagccgccc
     1921 gccttggtcg aaacgaacgg cttgaacagc ccggtgcgga cgaattcggc gctcatcccg
     1981 gaaccggaat cgaccacttc gatcacgccg tagaactgtt cgatcccgac ctggataaag
     2041 accgggctgt ctggcgggct cgcatcgatt gcgttctgaa tcaggtggac cagcacctgt
     2101 tcgatcacct cttcacgccc ggcgaccagg cacggttcgg cctggaccag ttcaaccggg
     2161 tggctcgcct tgaactgcgc cgccacccgc tttgccaccg cgtccgccgc gaccgg
//
LOCUS       DASUDV010000607         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1767132,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000607 DASUDV010000000
VERSION     DASUDV010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767132"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1126
                     /gene="prfA"
                     /locus_tag="VFV30_13405"
     CDS             53..1126
                     /gene="prfA"
                     /locus_tag="VFV30_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX4849135.1"
                     /translation="MSVSDARLAQIAARFAEIEARLASGTLEGKDFIAASRDYAELEP
                     VAKAAGLVEAMRAELVALEAMAELDDPEMREMAEEETRLLKDQLAVAEHELAIAMLPR
                     DSADSRSAMLEVRAGTGGDEAALFAADLLRMYERFAAEQGWRFELVSANASDIGGFKE
                     AVAEINGNGVFAKLKFESGVHRVQRVPVTESGGRIHTSAATVAVLPEPDEVDVQIEDK
                     DLKIDVYRASGAGGQHVNTTDSAVRITHLPTGTVVTCQDGRSQHKNKEKAMQVLRARL
                     FEAQREAVQGAEAEARKAMVGSGDRSERIRTYNFPQGRVTDHRINLTLHRLTEVLEGP
                     GLGELVDALIAEDQAKRLAALGE"
     gene            1123..1974
                     /gene="prmC"
                     /locus_tag="VFV30_13410"
     CDS             1123..1974
                     /gene="prmC"
                     /locus_tag="VFV30_13410"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX4849136.1"
                     /translation="MSVADAIREAAARLAGGSDTARLDAEVLMAHALGVSRSALLLHR
                     MHDPVPADYDALVERRIGHEPVAYITGGIEFFGREFVVSPAVLIPRGDSEVLVEAALA
                     AAPAPGRVLDCGTGSGALLLSVLAERTGAEGVGIDRSLDALQLAATNAARLGLADRAR
                     MVGADWDAPGWCDGLGRFDLILANPPYVEDTAELAPSVRAHEPAGALFAGADGLDAYR
                     VLIPQLPALLVEGGMALVEIGHRQAEAVGEMAMAAGFAVELHHDLAGRPRVLALQIPL
                     GNTGASA"
BASE COUNT          368 a          697 c          786 g          349 t
ORIGIN      
        1 ggggcgacgg tggtgaaatc gccactgccg tcgaaaatct tgtgttgaac tgatgtccgt
       61 ctccgacgcc cgtcttgccc agatcgctgc ccgcttcgcc gagatcgagg cgcggctggc
      121 ttcgggcacg cttgagggca aggacttcat cgccgccagc cgtgactatg ccgagctgga
      181 accggtggcc aaggccgcag gtctggtcga ggcgatgcgg gccgagctgg tggcgctaga
      241 ggccatggcc gagctcgacg atcccgaaat gcgcgagatg gccgaggagg aaacccggct
      301 gctcaaggat cagctggcgg tggccgagca cgaactggcg atcgccatgc tgccgcgcga
      361 ttcggctgac agccgctcgg ccatgctcga agtgcgcgcc ggaaccgggg gcgatgaagc
      421 cgcgctgttc gccgccgatc tcttgcggat gtacgaacgc tttgccgccg aacagggctg
      481 gcgcttcgag ctggtgagcg cgaacgccag cgacatcggc gggttcaagg aagccgtggc
      541 cgaaatcaac ggcaacgggg tgttcgccaa gctcaagttc gaaagcggcg tccaccgcgt
      601 gcagcgcgtg ccggtgaccg aaagcggcgg gcgcattcac acctcggcgg caacggttgc
      661 ggttctgccc gaacccgacg aggtcgatgt ccagatcgag gacaaggatc tcaagatcga
      721 cgtctaccgc gcctctggcg cgggcgggca gcacgtcaac accaccgatt cggcggtgcg
      781 gatcactcac ctgcccaccg gcacggtggt gacctgccag gacggccgca gccagcacaa
      841 gaacaaggaa aaggcgatgc aggttctgcg cgcccgcctg ttcgaggcgc agcgcgaagc
      901 ggtccagggg gcagaggccg aggcgcgcaa ggccatggtc ggctctggtg accgctcgga
      961 acggatccgc acctacaact tcccgcaggg gcgtgtcacc gatcaccgta tcaacctgac
     1021 cctgcaccgg ctgaccgaag tgctcgaagg gcccgggctt ggcgaactgg tcgatgcgct
     1081 gatcgccgag gatcaggcca agcggctggc ggcgctgggt gagtgagcgt cgctgacgcc
     1141 attcgcgaag cggccgcgcg cctcgccggc ggttcggaca cggcccggct cgatgccgag
     1201 gtgctgatgg cacacgcatt gggggtaagc cggtcggcgt tgttgctgca ccggatgcac
     1261 gatccggtgc ccgccgacta cgatgcgctg gtggaacgac ggatcggcca tgaaccggtg
     1321 gcctatatca ccggagggat cgaattcttc ggccgggagt tcgtggtttc gcccgcagtg
     1381 ctgattccgc gcggcgacag cgaagtgctg gtcgaggcgg cgctggctgc ggccccggcg
     1441 ccggggcgcg tgctcgattg cgggaccggc tcgggcgcgc ttctcctttc ggtcctggcc
     1501 gagcgaaccg gtgccgaagg cgtggggatc gaccgatcgc tcgatgccct gcaactggcg
     1561 gcaaccaatg ccgcccggct cggcctggcc gatcgggcgc ggatggtcgg cgccgactgg
     1621 gatgcgcccg gctggtgtga tggccttggc cggttcgatc tgatcctggc caacccgccc
     1681 tatgtcgagg atacggccga gcttgcccct tcggtgcgcg cgcacgaacc ggccggcgcg
     1741 ctgtttgccg gggccgatgg gctagatgcc taccgggtgt tgatcccgca actgcccgcg
     1801 ctgctggttg aaggcggcat ggcgctggtc gagatcggcc atcgccaggc cgaagcggta
     1861 ggcgaaatgg ccatggctgc cggtttcgcg gtggagctgc accacgatct ggccggaagg
     1921 ccccgggtgc tggcgctgca aattccgctt ggcaacaccg gcgccagcgc gtaagcgcat
     1981 gggcaggcca gcgcaggaac gcatcgtttt gcgtcccgca ggccaaatcc agcatttgcg
     2041 acccgcgcgg caaacccgcc ccgctacctt gcagatgtcg gaccccggca cacaatggcg
     2101 tggccgggtc aggatgggtc atgcagccgt cgcggattag aaccccgggc ctggctgccg
     2161 gattagggaa ttgacaccct tgaataacaa tcgtgggaat
//
LOCUS       DASUDV010000608         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_441369,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000608 DASUDV010000000
VERSION     DASUDV010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441369"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFV30_13415"
     CDS             complement(<1..679)
                     /locus_tag="VFV30_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4849137.1"
                     /translation="MPGSPDATAPVAVAEAPIDEPTMPWSLDNAKALLKVIQGIEAEG
                     LIPADYQLQALQTAIAAGPGAELDAQASRSFAWLVEDMRDGRTRMDSRVQWFVVDTDI
                     ETSPTSALMAQALASGDIAGTIAGLAPTAPDYSVLKATLAATPKAQKTKRNLIRVNLD
                     RWRWLPRDLGALHLMTNVPEFQLRVVKEQQVVLTYRTVVGKPGKTATPQLAEKVTAVV
                     FNPTWTVP"
     gene            complement(1014..2039)
                     /locus_tag="VFV30_13420"
     CDS             complement(1014..2039)
                     /locus_tag="VFV30_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4849138.1"
                     /translation="MALPRCQCGSIGAEAGMYTLYGALGSPYSMKLRALLRYRRIPFI
                     WKQGPEVRAALGKVKAPVIPVLEYPDGHCANDSTPLIYDLEARDPHRGVVPPDPAQAF
                     IAHLIEDFADEWLTKAMFGYRWLEEVDQVQMSRWLAFDNLKGGGLAQSQGFAEQFRAR
                     QVGRMALVGCTAENFPLIEASTRAVLAALEAHVVERHCLFGTRPSLAEFGLFGQLSQL
                     GTDPTAQAMMRADYPYTYRWLAHVDDLSGIEGDWETGFAPVVAQLVRIAGEVYAPFLA
                     ANAAALDAGEDHFEMVAMGLPYSQGSFKYQARCLADLRARYGELGGAARNLVDPLLGP
                     AWTSLLQ"
     gene            complement(1999..3210)
                     /locus_tag="VFV30_13425"
     CDS             complement(1999..3210)
                     /locus_tag="VFV30_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX4849139.1"
                     /translation="MAERAISPVDLSDAALYTEDRWREPFAHLRGEAPVSWREESPYG
                     GYWSVVGHELIQAVELDPVTYSSQIGNITIADGVAGQEFPNFIAMDPPRHTEQRRVVA
                     AAFTPSQMALREKQVRARTRELFDALPVGETFDWVDRVSVPVTIAMLAIVLDFPWDER
                     MNLRKWSDMASNVSPEVMTEDYAAQFFAEMGLMLARFDELLEARRTQEPADDLLSRMI
                     HSEAMGNLTPLERIANIALLIVGGNDTTRNSISGFVEALHKYPGELERLRADPSLIPN
                     AAQEIIRWQSPVTHMRRTMTRDVELAGQQLKAGEKIVLWYLAANRDESVFADAERFDV
                     GRENARRHLGFGHGIHRCVGARLAEIQLCTVIEEIVERGWRIVPQGAPTRLASPFLHG
                     FTAMPVRIDRG"
     gene            3389..3790
                     /locus_tag="VFV30_13430"
     CDS             3389..3790
                     /locus_tag="VFV30_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849140.1"
                     /translation="MTGQIDATAWTGLLLGLTALFAGIGALRKPGAWRTMLDEVERSP
                     ALQFVCGMLELLIGALVYLANPWLPADLMTCVLKALGGAMMFEALAILGFVDIYSQFW
                     LRTLTHMHRGWALTTTLFGLVLTAISLARFN"
     gene            3873..4565
                     /locus_tag="VFV30_13435"
     CDS             3873..4565
                     /locus_tag="VFV30_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849141.1"
                     /translation="MNRTILGCALLALATAPAHARERTPAQGFANPSAVIAAELAFAK
                     LAQEKGQWTAFRETATKDAVMFVPQMVLAQEHLKDKADPANPVKWQPHQVWSSCDGSI
                     GVTRGAWQAGEATGWFTTIWQRQQKGQYKWIMDQGDALPMPLDPPDMIVAKVADCPAG
                     YRGSRQKRKDVKGKVVLGDPTQRSGQSDDGTLSWEVKVEPDGARRFAMALKLDGQMTP
                     VQVVEVAAPEKK"
     gene            4576..>4988
                     /locus_tag="VFV30_13440"
     CDS             4576..>4988
                     /locus_tag="VFV30_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX4849142.1"
                     /translation="MIDLFLSAFITLFVVIDPPGCAPIYAGLVKDASNRQAASMALRA
                     CIIATIILVIFALFGEDLLGALHIELNSFRLAGGIMLFVIALDMVFEKRTQRREERAE
                     KVRAHNAQTPEVEDVSVFPMAMPMIAGPGSIATMML"
BASE COUNT          866 a         1596 c         1661 g          865 t
ORIGIN      
        1 gcggcacggt ccaggtgggg ttgaacacca ctgccgtaac cttttcggcc agctgcgggg
       61 ttgcggtctt gcccggcttg cccaccacgg tgcgataggt cagcacaacc tgctgttcct
      121 tgaccacgcg cagctggaat tcgggcacgt tggtcatcag gtgcaaggcg cccagatcgc
      181 gcggcaacca gcgccagcga tcgaggttga cgcggatcag gttgcgcttg gtcttctgcg
      241 ccttgggcgt tgccgccagc gttgccttga gcacgctgta gtcgggtgcg gtcggggcca
      301 ggccggcgat cgttccggca atgtcgccgc tggcgagcgc ctgggccatc agcgccgagg
      361 tcgggctggt ttcgatatcg gtatcgacca cgaaccactg cacccgcgaa tccatccggg
      421 tgcggccatc gcgcatatcc tcgaccagcc aggcgaacga acggctggcc tgggcgtcga
      481 gttcggcacc gggaccggcg gcgatcgccg tctgcaatgc ctgaagctga taatcggccg
      541 gaatcagccc ttcggcctcg atcccctgga tcaccttgag cagggctttg gcattgtcga
      601 gcgaccaggg catggtcggc tcgtcgatcg gggcttcggc aacggcaacc ggcgcggtcg
      661 catccggaga tccgggcacc gcagtcgcgg cggggccggg cgaaggggct gacgttgggg
      721 ccggggtggc tgaaggcgcg ggtgccgggc tgggtgcggg ggcaggcggc aggatcgatt
      781 ggggctgtgc ggcagaaagc gccgcagccg aagtgagccc gaaccccaga ccgacaatcc
      841 ggcccaaagt catcatgccc cggtaacgca tcaaaatctc cagcctttcc tggcgcctgg
      901 cccggcaagg ttgcggaccc atggcacact aatgcccgtt tcgctgcgct gcatcaagca
      961 tggcagcttt cgcggcgctg aaccggttgc gattttcgcc gatcggggtg atcttattgc
     1021 agaaggctgg tccaggcggg gcccagcagc ggatcgacca ggttgcgcgc ggcaccgcca
     1081 agttcgccat agcgcgcgcg caggtccgcc aggcaccggg cctggtactt gaagctgccc
     1141 tggctgtagg gcaggcccat tgccaccatc tcgaaatggt cctcgccggc atccagcgcc
     1201 gcggcattgg cggccaggaa cggggcatag acctcgcccg cgatccgcac cagctgcgcc
     1261 accacgggcg caaagccggt ttcccagtcg ccctcgatcc ccgaaagatc atcgacatga
     1321 gccagccagc gataggtgta gggatagtcg gcgcgcatca tcgcctgggc cgtggggtcg
     1381 gtgccaagct gcgaaagctg cccgaacagg ccgaattcgg ccaggctcgg ccgggttccg
     1441 aacaggcagt ggcgctccac cacatgggct tcgagcgcgg ccagaaccgc gcgggtgctg
     1501 gcctcgatca gcggaaaatt ctcggcggtg cagccgacca gcgccatccg cccgacctgg
     1561 cgggcgcgga actgttcggc aaagccctgg ctttgcgcca ggccgccacc cttcaggttg
     1621 tcgaaggcca gccagcggct catctgcacc tggtcgacct cttccagcca gcggtagccg
     1681 aacatcgcct tggtcagcca ctcatcggca aaatcctcga tcagatgggc gatgaaggcc
     1741 tgcgcgggat cgggcggaac cacgccgcgg tgcggatcgc gcgcttccag atcgtagatc
     1801 agcggggtcg aatcgttggc gcagtgtcca tccggatatt ccagcaccgg gatcaccggt
     1861 gccttgacct tgcccagcgc cgcgcgcacc tctggcccct gcttccagat gaacgggatc
     1921 cggcgataac gcagcagcgc ccgcagcttc atcgaatagg gcgaacccaa ggcgccatag
     1981 agcgtgtaca tgcccgcctc agccccgatc gatccgcact ggcatcgcgg taaagccatg
     2041 caggaacggg ctggcaaggc gcgtgggggc gccctggggc acgatccgcc agccgcgctc
     2101 gacgatttcc tcgatcaccg tgcaaagctg gatctcggcg aggcgtgcgc ccacgcagcg
     2161 gtgaatgcca tggccgaagc ccaggtgccg gcgcgcattc tcgcggccca cgtcgaagcg
     2221 ttcggcatcg gcgaacacgc tttcatcgcg attggcggca agataccaca gcacgatctt
     2281 ctcgcccgcc ttgagctgct ggccggccag ttcgacatcg cgggtcatgg tccggcgcat
     2341 gtgcgttacc gggctttgcc agcggatgat ttcctgcgcg gcattgggga tcagcgaagg
     2401 atcggcgcgc agccgctcaa gctcgcctgg gtacttgtgc agcgcctcga caaagccgct
     2461 gatcgaattg cgggtggtgt cattgccccc gacgatcagc agggcaatat tggcgatccg
     2521 ctcaagcggg gtgaggttgc ccatggcttc cgaatggatc atccggctca gcaagtcgtc
     2581 cgccggttcc tgcgtgcgcc gggcttcgag cagctcgtcg aatctggcca gcatcaggcc
     2641 catctcggca aagaactgtg cggcatagtc ttcggtcatc acctcgggcg agacgttgct
     2701 ggccatgtcc gaccacttgc gcaggttcat ccgctcgtcc caggggaagt cgagcacgat
     2761 ggccagcatc gcgatggtca ctggaaccga aacccgatcg acccagtcaa aggtttcgcc
     2821 caccggcagg gcatcaaaca gctcgcgggt gcgggcgcga acctgctttt cgcgcagcgc
     2881 catctggctg ggggtgaagg cggcagccac cacccggcgc tgttcggtgt ggcgcggcgg
     2941 gtccatcgcg atgaaattgg ggaattcctg cccggccacg ccatcggcaa tggtgatatt
     3001 gccgatctgc gaggaatagg tgaccggatc aagctcgacc gcctggatca gttcgtggcc
     3061 aaccaccgac cagtagccgc cataagggct ttcctcgcgc cacgagaccg gtgcttcgcc
     3121 gcgcaggtgc gcaaacggct cgcgccagcg atcttcggtg tacagcgcag cgtcgctcag
     3181 atcgaccggg gagatcgcgc gttcggccat ggacgggttg ttcctttgct gaagggactg
     3241 ggtgaaccgc ggcggagtat gtttaagcat ccgattaatg tccagcacgg atcgggggct
     3301 ggacaagcgg cgcgccgcct tccatcctgc gtctcataaa ggggacaacc cgggtgggga
     3361 gaaacaccga aacgggttgg aggaagggat gactggacaa attgacgcaa ctgcctggac
     3421 cgggctgttg ctggggctga ccgcgctgtt tgccgggatc ggtgcgctgc gcaagccagg
     3481 cgcctggcgg accatgctgg acgaggtcga gcgttcaccc gcgctgcaat tcgtctgcgg
     3541 gatgcttgaa ctcctgatcg gggcgctggt ctatctggcc aacccctggc tgccggctga
     3601 tctcatgacc tgcgtgctca aggcgctggg cggggcaatg atgttcgagg cgctggcgat
     3661 ccttggcttc gtcgacatct acagccagtt ctggctgcgc acgcttacgc atatgcatcg
     3721 cggctgggcc ttgaccacga ccctgttcgg tttggtgctg acggcgatct cgctcgcgcg
     3781 cttcaactag gagccgcgac cgcgagcgcc cgaaattgcc ttttgcccgc gcgctgttgg
     3841 cgcgcaggca aagccgggct agtctggggg ccatgaacag aacgattctc ggttgcgcgc
     3901 ttctcgcact cgccacggcc cccgcgcatg cgcgcgaacg cacgccggcc cagggctttg
     3961 ccaatcccag tgcggtgatc gccgccgaac tggcctttgc caagctggcc caggaaaagg
     4021 gacagtggac tgcgtttcgc gaaaccgcca ccaaggatgc ggtcatgttc gtgccgcaaa
     4081 tggtgctggc gcaggaacac ctcaaggaca aggccgatcc cgccaacccg gtgaagtggc
     4141 agccgcacca ggtctggtcg agctgcgacg gttcgatcgg ggttacccgc ggcgcctggc
     4201 aggcgggtga ggctaccggc tggttcacca cgatctggca gcgccagcag aagggccagt
     4261 acaagtggat catggaccag ggcgatgccc tgccgatgcc gctcgacccg cccgacatga
     4321 tcgttgccaa ggtggccgac tgcccggcag gctatcgtgg ttccaggcaa aagcgcaagg
     4381 atgtgaaggg caaggttgtg ctgggcgatc cgacgcagcg cagcggccag tcggacgatg
     4441 gcacgctaag ctgggaagtg aaggtcgagc ccgatggcgc gcggcgcttt gcgatggcgc
     4501 tcaagctcga tggccagatg accccggttc aggtggtcga agtggccgcg cccgaaaaga
     4561 agtgatcgcc cgggcgtgat cgacctcttc ctctccgcct tcatcaccct gttcgtggtg
     4621 atagacccgc ccggctgcgc gccgatctat gccggtctgg tcaaggatgc cagcaatcgc
     4681 caggccgcct cgatggcact gcgcgcctgc atcatcgcca cgatcatcct ggtgatcttc
     4741 gcactgttcg gcgaggatct gttgggcgcg ctccatatcg agctcaactc gttccggctg
     4801 gccggcggga tcatgctgtt cgtgatcgcg ctcgacatgg tgttcgaaaa acgcacccag
     4861 cgccgcgagg aacgcgccga aaaggtccgc gcgcacaatg cccagacgcc cgaggtcgaa
     4921 gatgtctcgg tcttcccgat ggccatgccg atgatcgccg ggcccggttc gatcgccacc
     4981 atgatgct
//
LOCUS       DASUDV010000609         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1767240,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000609 DASUDV010000000
VERSION     DASUDV010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767240"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFV30_13445"
     CDS             complement(<1..1326)
                     /locus_tag="VFV30_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4849143.1"
                     /translation="MKSFARGCTVMVGMVAVTYHAPQAAQSIPTVDAPKPPPVVVMPA
                     PPPPIMIAPPAPPAPPSYGPAPKAAPALPIPADPKAAKDQAKAARKAREADGPACANR
                     DALKPWMIKTMIAACDRLLAMPTDPANPWTWRAEVLQYRALALVNAGDLKNAIDAIDE
                     SDAIGAIQPDPLFDLSIGIGNKMIRAYIMGRRGETKAARELLAQVRQQRPWSTTVVAG
                     ADAIELGMLSGDSEEITRRIGARYRIDPDVARMLIYLNLMRGDLKGAADMGSRVSMVD
                     PRMRGGWTLQGGGSEDENLRNRVRIDCTKAYVAAALGDKAGSAAQFAAIEQFIAGYIG
                     PDPRNVDPKLRPDKSQIKRFEERLATGTKLRAMVADWQDVTAKRDSFAEKDFKALSER
                     FKAYPSSADLLPALFDQGRVLNARQTGKEAAETKLFLDGFLRGLTGSITR"
     gene            1425..>2174
                     /locus_tag="VFV30_13450"
     CDS             1425..>2174
                     /locus_tag="VFV30_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4849144.1"
                     /translation="MLRLAAFFVMSCLALAGSAVAAPPRKPAKPVTIFVPPPAPVEKP
                     EVLAERGKQAMEAKNYAGAARDIELACAAGIAESCYYQGELLRTGAAGKRDIDAALSA
                     YRKACEAGDARSCNSFAVNQLPFARDDAARAQNTAFLARACAMDHGQACSNLAYRIEK
                     GTGIAQDKVEALRLYRKACDLKYAGGCRNAAISLEAGNGAEKDLAGALALYQKGCELD
                     ELDSCFWAAVAIEEGKGTTPDKITARGLYGKN"
BASE COUNT          376 a          701 c          709 g          388 t
ORIGIN      
        1 gcgcgtgatc gaaccggtca gtccgcgcag gaagccatcg aggaacagct tggtttcggc
       61 cgcttccttg ccggtctggc gcgcgttgag gacccgcccc tggtcgaaca gcgcgggcag
      121 cagatcggca ctgctgggat aggccttgaa gcgttccgaa agcgccttga agtccttttc
      181 ggcaaagctg tcgcgcttgg cggtaacgtc ctgccagtcg gcaaccattg cccgcagctt
      241 ggtaccggtg gccaggcgtt cttcaaagcg cttgatctgc gacttgtcgg ggcgaagctt
      301 gggatcgacg ttgcgtggat cgggcccgat gtatccggca atgaactgct cgatcgccgc
      361 gaactgcgcc gccgatccgg ccttgtcgcc cagggctgcc gcgacatagg ccttggtgca
      421 atcgatccgc acccggttgc gcagattttc atcttccgag ccgccgccct gcagcgtcca
      481 gccgccgcgc atccgcggat cgaccatcga aacccggcta cccatgtcgg ccgccccctt
      541 caggtcgccg cgcatcaggt tgaggtagat cagcatgcgg gcaacgtccg gatcgatccg
      601 gtagcgcgcc ccgatccggc gcgtaatctc ttcactgtcg ccactgagca tgcccagttc
      661 gatcgcgtcc gcaccggcaa ccaccgtggt cgaccagggg cgctgctggc gaacctgggc
      721 gagcagttcc ctggccgcct tcgtttcacc ccggcgcccc atgatatagg cgcggatcat
      781 cttgttgccg attccgattg agaggtcgaa caagggatcg ggctgaatgg cgcctatcgc
      841 gtcggattcg tcgattgcat cgatcgcgtt cttcaaatcg ccggcattga ccagcgccag
      901 cgcccggtac tgcagcactt cggcacgcca tgtccaggga ttggccggat cggtcggcat
      961 ggccagcaac cggtcacagg cggctatcat cgtcttgatc atccagggct tgagcgcatc
     1021 gcggttggcg caggccgggc catcggcctc gcgcgccttg cgggcggcct tggcctggtc
     1081 cttggctgcc ttgggatcgg cgggtatcgg cagcgccggc gcggccttgg gcgcggggcc
     1141 atagcttggc ggcgcgggtg gcgctggcgg ggcaatcatg atcggcgggg gcggcgcggg
     1201 catcacgact accggcggcg gctttggcgc atcgaccgtg ggaattgact gcgcggcctg
     1261 cggcgcatgg taggtgacgg ccaccatgcc gaccattact gtacaacccc gcgcgaacga
     1321 cttcatccca caagctccat caagtccttg tcgaggaatt gtttctattg accaagtcga
     1381 gcgcgcaagg aaaataatcg cctattcgca acaggagcca cccaatgctg cgcctggccg
     1441 ctttcttcgt catgtcgtgc cttgctcttg ccgggtcggc cgttgcagcc ccgccgcgca
     1501 agccggcaaa gccggtcaca atcttcgtgc cgcccccggc tccggtcgaa aaacccgagg
     1561 tgctggccga acgcggcaag caggcgatgg aggcgaagaa ctatgccggc gcggcaaggg
     1621 atatcgaact ggcctgcgcc gccgggatcg ccgaaagctg ctattatcag ggcgaactgc
     1681 tgcgcaccgg tgcggcggga aagcgggaca tcgacgctgc gctgagcgcc tatcgcaagg
     1741 cctgcgaagc gggcgatgcc cggtcttgca acagctttgc ggtcaaccag ttgccgtttg
     1801 cccgcgatga tgcggccagg gcccagaaca ccgcgttcct ggccagggcc tgcgcgatgg
     1861 accacggcca ggcctgctcg aacctggcct atcgcatcga aaagggaacc ggcatagccc
     1921 aggacaaggt cgaggcgctg cggctctatc gcaaggcatg tgacctcaaa tatgccggag
     1981 gctgccgcaa tgccgccatt tcgcttgaag cgggcaatgg cgccgaaaag gacctggccg
     2041 gagcgctggc gctttaccag aagggctgcg agctcgatga acttgacagt tgcttctggg
     2101 cggccgtcgc gatcgaggaa ggcaagggca ccactcccga caagatcacc gcgcgcggcc
     2161 tctatggcaa gaac
//
LOCUS       DASUDV010000610         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Novosphingobium sp. isolate SMAG_U1335 k141_1480063,
            whole genome shotgun sequence.
ACCESSION   DASUDV010000610 DASUDV010000000
VERSION     DASUDV010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961488
            Sequence Read Archive: SRR25158408
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Novosphingobium sp. (soil metagenome)
  ORGANISM  Novosphingobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
            Sphingomonadaceae; Novosphingobium.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:07:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,692
            CDSs (total)                      :: 2,662
            Genes (coding)                    :: 2,651
            CDSs (with protein)               :: 2,651
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Novosphingobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480063"
                     /isolate="SMAG_U1335"
                     /isolation_source="soil"
                     /db_xref="taxon:1874826"
                     /environmental_sample
                     /country="China"
                     /lat_lon="28.61222222 N 115.8344444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="ispH"
                     /locus_tag="VFV30_13455"
     CDS             complement(<1..936)
                     /gene="ispH"
                     /locus_tag="VFV30_13455"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX4849145.1"
                     /translation="MAASSITPADPHPEPVRRRVLLANPRGFCAGVERAIEAVEQALA
                     RHGAPVFVRRAIVHNREVVARLERQGAIFVQEIVEIPEGAVAILSAHGSARSVKLAAQ
                     GRSLRTVDAICPLVAKVHAEVEAWYRAGRHVLLIGHSGHPEIVGTLGQVPAGAVSVVS
                     QPGDLDALDLPPNTAVAYAVQTTFAMRDADGMIAAIKARFGDVAGPRASDICYATTNR
                     QQAIEGLAAKADLVLVVGDTMSSNARRLVEVALAAGAPAARLVSGPDDLPRPELQGAQ
                     TVALTAAASAPESAVRAVCAVLEQQGFDLAEADGSP"
     gene            1038..2510
                     /locus_tag="VFV30_13460"
     CDS             1038..2510
                     /locus_tag="VFV30_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEX4849146.1"
                     /translation="MANQNSALVIGSGFGGLALAIRLQSAGIATTIVEARDRPGGRAY
                     VWKQDGYTFDAGPTVITDPACLEELWGLSGSRMADDVTLLPVNPFYRLLWNDGTRFDY
                     SNDDPQLNAEIAKLNPADVAGYARFLEYSKGVYEEGYLKLGAVPFLDFASMLKAAPAL
                     ARYQAWRSVYSMVSSFVENDKLRQALSFHSLLVGGNPMNTSSIYALIHRLEKLGGVWF
                     AKGGTNALVSGMVALFERLGGTLLLGEKVARITHQGKRVTGVVTEGGRSLSADMVASN
                     GDLVHTYGLIDGSSYAAGKVRSLHRKRFSPSLFVVHFGLNGSFPDIAHHSILFSDRYG
                     PLLKDIYKHGKLADDPSLYLHHPSATDPDMAPAGKAAFYALAPVPHMGRAPVDWAVEG
                     PRYRDVVLGRIRDLLIPDLDDRLDTCFHYTPQDFHDDLGAHLGSAFSLEPVLWQSAWF
                     RGHNRDDHFPNLYFVGAGTHPGAGIPGVVGSAKATASLIV"
BASE COUNT          404 a          872 c          845 g          416 t
ORIGIN      
        1 ggggctgccg tcggcttcgg ccaggtcaaa gccctgctgt tccagcacgg cgcaaaccgc
       61 gcgcaccgcg ctttccgggg cggaggcggc ggcggtcagg gctacggtct gcgcgccttg
      121 cagttcgggc cggggcaggt catccggacc ggagaccagc cgcgcggctg gcgccccggc
      181 ggcaagcgcc acctcgacca gtcggcgcgc gttggacgac atcgtgtcgc ccaccaccag
      241 gaccaggtcg gccttggccg ccagcccctc gatcgcctgc tggcggttgg tggtggcata
      301 gcagatgtcg ctggcccgcg gcccggcgac atcgccgaac cgggccttga tcgcggcgat
      361 catcccgtcg gcatcgcgca tcgcgaacgt ggtctgcacg gcataggcca ccgccgtgtt
      421 gggcggcagg tcgagcgcgt cgagatcgcc cggctgggat acgaccgata cggccccggc
      481 gggcacctgg cccagcgtgc cgacaatctc gggatgcccg gaatggccga tcagcagcac
      541 gtggcggccg gcgcggtacc aggcttcgac ttcggcatga accttggcca ccagcgggca
      601 gatggcgtct acggtccgaa ggctgcgacc ctgggctgca agcttgaccg aacgggcgct
      661 gccatgggcg gaaaggatcg caaccgcgcc ttcggggatc tcgacgatct cctgaacgaa
      721 gattgcgccc tgccgttcga gccgtgccac cacctcgcgg ttgtggacga tcgcgcggcg
      781 cacgaagacc ggggccccgt gccgggcaag cgcctgttcg accgcttcga tcgcccgctc
      841 tactccggca cagaatccgc gcggattggc cagcagcaca cgccgccgga caggttccgg
      901 gtggggatcg gccggggtga tcgaactcgc agccatacgt ttccttcagg cttgcacctt
      961 gcctatgggg tcccgggctt ggtatccgcc ctttgggaca ggttgtccac aaccgggcaa
     1021 gggatcaatc ggccaccatg gcgaaccaga attccgcgct cgtgatcgga tccggctttg
     1081 gcggactggc gctggcgatc cgcctgcaaa gcgccggcat cgccaccacg attgtcgagg
     1141 cgcgcgaccg gcccggcggg cgcgcctatg tctggaaaca ggacggctac accttcgatg
     1201 ccgggccgac ggtgatcacc gatccggcct gcctggaaga gctgtggggc ctgtcgggca
     1261 gccggatggc cgacgatgtc acgctgctgc cggtgaaccc gttctatcgg ctcctgtgga
     1321 acgacggcac ccggttcgac tattcgaacg acgatcccca gctcaacgcc gaaatcgcca
     1381 agctcaatcc cgccgatgtc gccggctatg cccggttcct cgaatactcc aagggggtct
     1441 acgaggaagg ctatctcaag ctgggcgcgg tgccgtttct cgatttcgcc tcgatgctga
     1501 aggccgcccc ggcgctggcc cgctaccagg cctggcgctc ggtctattcg atggtgtcga
     1561 gcttcgtgga gaacgacaag ctgcgccagg cgctctcgtt ccacagcctg ctggttggcg
     1621 gaaatccgat gaacaccagc agcatctatg ccctgatcca ccggctggaa aagctgggcg
     1681 gggtgtggtt cgccaagggc gggaccaatg cgctggtcag cggcatggtc gcgctgttcg
     1741 aacggctggg cggcacgctg ctgctgggcg aaaaggttgc gcggatcacc caccagggca
     1801 agcgggtgac cggcgtggtg accgagggcg gacgcagcct gagcgccgac atggtcgcca
     1861 gcaacggcga tctggtccac acctacggcc tgatcgatgg gtcgtcatat gccgcgggca
     1921 aggtccgctc gctgcaccgc aagcggttct cacccagcct gttcgtggtc catttcgggc
     1981 tgaacggcag cttccccgac attgcccacc actcgatcct gttttcggac cgctacggcc
     2041 cgctgctcaa ggacatctac aagcacggca agctggcgga cgatccctcg ctttacctcc
     2101 accaccccag cgcgaccgat ccggacatgg ccccggccgg caaggcggcc ttctatgccc
     2161 tggccccggt gccacacatg ggccgcgctc cggtggactg ggcggtcgaa gggccgcgct
     2221 accgcgacgt ggtgctcggc cggatccgcg acctgctgat ccccgatctc gatgaccggc
     2281 tcgacacctg cttccactac accccgcagg acttccacga cgatctgggc gcgcacctcg
     2341 gcagcgcctt cagcctggag ccggtgctgt ggcaaagcgc gtggttccgc ggccacaacc
     2401 gcgacgatca tttcccgaac ctctatttcg tcggtgcggg gacccatccc ggtgccggga
     2461 ttccgggcgt ggtcggcagc gccaaggcca cggccagcct gatcgtctga cgctgcaaaa
     2521 ggtgctatag gccgccg
//
